LOCUS       DBNFEG010000001        38933 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.001, whole genome shotgun sequence.
ACCESSION   DBNFEG010000001 DBNFEG010000000
VERSION     DBNFEG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 38933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38933
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.001"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            164..2509
                     /gene="pflB"
                     /locus_tag="AC1PNG_000001"
     CDS             164..2509
                     /gene="pflB"
                     /locus_tag="AC1PNG_000001"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HIP6700547.1"
                     /translation="MSTKVKTKNVAEDIFAQAWEGFKGTDWQDKASVTRFVQANYTPY
                     DGDESFLAGPTERSLHIKKIIEETKAGYEDTRFPMDVDRATSIADIPAGFIDKENELI
                     FGIQNDELFKLNFMPRGGIRMAETTLIENGYTPDPLLHEIYTKHATTVNDGIFRAYTA
                     DIRRSRHSHHVSGLPDAYSRGRIIGMYARLALYGADYLMEEKVADWNAITEIDEESIR
                     LREEINMQYQALQQVVRLGDHYGVDVRKPAMNTKEAIQWTNIAFMAVCRVINGAATSL
                     GRVPIVLDIYAERDLARGTFTESEIQEFVDDFVLKLRTVKFARTKAFDEIYSGDPTFL
                     TTSMAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWSDQLPYSFRRYCMAMSH
                     KHSSIQYEGVTTMAKDGYGEMSCISCCVSPLDPESEDQRHNIQYFGARVNVLKALLSS
                     WNNGYDDVHKDYKVFDGVEPNTSEVFDYDQVVKNFEIALDWLTDTYVDAMNIIHYMTD
                     KYNYEAVQMAFLPTHLRANMGFGICGFANTVDSLSAIKYAQVKPIRDEDGFIYDYEVT
                     GDFPRYGEDDDRVDDIAKWLMEAFFTRLNKHKLYKNAEATVSILTITSNVAYSKQTGN
                     SPVHRGVFLNEDGSVNTTKVEFFPPGANPTSKARGGWLQNLNTLSKLNFKHANDGISL
                     TTQVSPKALGKTFDEQVNNLVTILDGYFEQGGQHVNLNVMDLNDVYEKIMAGEDVIVR
                     ISGYCVNTKYLTKEQKTELTQRVFHEVLSMDDHAAEVSGQA"
     gene            complement(2692..3483)
                     /locus_tag="AC1PNG_000002"
     CDS             complement(2692..3483)
                     /locus_tag="AC1PNG_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COG3942 and LysM peptidoglycan-binding
                     domain-containing protein"
                     /protein_id="HIP6700548.1"
                     /translation="MKKFSCKSVLALAGITVALSTISRVQATSHVVMAGDSMFSIAEQ
                     YGVDPYQLAEVNAMEIDGLITPGQRLDLPAGSNEGVSVAGQTDYVVQDGDSFYGIADA
                     FSLDINKLLEQNQLTLDANIHPGQILKLTETIWNQTVDTAADSYYLPGYEYEPGINYP
                     VGQCTWGVQKVAPWAGDWWGDAASWGANAARDGFRTGTTPEVGAIISWNDGALGHVAY
                     VTAVNEDGQIQVLEANYRGQQWVDNFRGWFNPTDTIGEITYIYNN"
     gene            complement(3565..4116)
                     /locus_tag="AC1PNG_000003"
     CDS             complement(3565..4116)
                     /locus_tag="AC1PNG_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HIP6700549.1"
                     /translation="MRYVLLLRGINVGGRNKIVMAELRQAVADLGYDKVETYINSGNL
                     FFDSTKNRGDIVAEFQTFFTERYPFVEQFALLDTQDYQAEIDQLPAWWQEELARKDVL
                     FFTEGLDKQAMVDYIQSLHLGDEVLHFSDYAIYWGKYTEGSYLQTAYHKQLAKSPFYK
                     QVTIRNGKTFSVISNYLENEIKR"
     gene            complement(4113..5054)
                     /locus_tag="AC1PNG_000004"
     CDS             complement(4113..5054)
                     /locus_tag="AC1PNG_000004"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIP6700550.1"
                     /translation="MRRVILDKVSEQIEQGVYPGASLAFFSEGQWQEYYLGTQDGHTP
                     VEAGLTYDLASVSKVVGVGTLATFLVNSGALELDRTLQSYYPAFADSEVTIRQLLTHT
                     SGIDPFIPNRDSLDADQLKEAILKIAVTDKKAFLYTDINFLLLGFLLEELGRDSLDQL
                     ISRDVLEPFGMSQTSFGPVSQAVPTVRGVKAGVVHDPKARVLGLHAGSAGLFSTVKDL
                     EIFLEHYLMADFAANLTQDYGFDVKRKRSLAWDKKGDWLSHTGYTGTFIMYNRPLQKA
                     AIFLSNRTFDKDERAQWKLDRNQVMALIRQVLEEEGQ"
     gene            complement(5051..5797)
                     /locus_tag="AC1PNG_000005"
     CDS             complement(5051..5797)
                     /locus_tag="AC1PNG_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HIP6700551.1"
                     /translation="MKCIVLRKNEAIVPVLRINNRAINQGFLEKNLGMKTKLEDGPFA
                     DFGDRTNPETKLVLTESPGNRTRAVEGLKKLNKIVIKVDNASEIEALLAQGSQYSKLY
                     QGKNGYGFEAVSPEGDTFLLHAEASVDDLKAILPPVPFKVQDDFSGLTAFTVESVWIN
                     TPQASINQDYYEAILPQQAFLRFIGAEGEDLLTPAEQVWDLEGLRFPVEQDFDWAHLE
                     SRLEGPFFKDKKASFIQTVDPSGIELWFEK"
     gene            5967..8234
                     /locus_tag="AC1PNG_000006"
     CDS             5967..8234
                     /locus_tag="AC1PNG_000006"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HIP6700552.1"
                     /translation="MRFNQFSFIKKETSVYLQELDALGFQLIPNVSSKTNLETFVRKC
                     HFLTANTDFALSNMIADWDTDLLTFFQSDRELTDLIFYQVAFQLLGFVPGVDYTDVMD
                     FVEKSNFPVIYGDMIDNLYQLLATRTKSGNTLIDQLVSDDLIPEDNRYHFFNGKSLAT
                     FSTKNLIREVVYVETPVDTAGTGQTDIVKLSILRPHFDGKIPAVITNSPYHQGVNDVA
                     SDKALHKMEGELAEKQVGTIQVKQASITKLDLDQRELPVSPSTEKLGHITSYSLNDYF
                     LARGFASLHVSGVGTLGSTGYMTSGDYQQVEGYKAVIDWLNGRTKAYTDHTRSIEVKA
                     DWANGKVATTGLSYLGTMSNALATTGVDGLEVVIAEAGISSWYDYYRENGLVTSPGGY
                     PGEDLDSLTALTYSKSLQAGDFLRSKEAYEKGLAAERAALDRTSGDYNQYWQDRNYLL
                     QADKVKCEVVFTHGSQDWNVKPIHVWNMFHALPSHVKKHLFFHNGAHVYMNNWQSIDF
                     RESMNALLSQKLLGYENNYQLPTVIWQDNSGEQTWTTLDTFGGENEAVLPLGTGSHTI
                     ANQYAQEDFDRYGKSYPAFHQDLYAGKGNQVSIDLPVTEDLLLNGQVTLKLRVASSVA
                     KGLLSAQLLDKGNKKRLAPIPAPKARLSLDNGRYHAQENLLELPYVDMPQRLVTKGFM
                     NLQNRTDLMTVEEVVPGQWMNLTWKLQPTIYQLKKGDVLELILYTTDFECTVRDNSQW
                     QIHLDLSQSQLILPH"
     gene            complement(8514..10922)
                     /locus_tag="AC1PNG_000007"
     CDS             complement(8514..10922)
                     /locus_tag="AC1PNG_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HIP6700553.1"
                     /translation="METANKKFRYSIRKFKVGVGSVLIATCLLGAGVSTPTAFATTET
                     STATQVEAAQPTSETEPTVQITGTVSTENTNSESKVAPEVKEEAPVAEKAETATNSAE
                     QNVVSEPEKSEEPTPAPAALTSEAKEEVSENTEVKSAPLPQFQDILKELERDLEELKV
                     FRDYTGSDYSFQKGLWTYLLEDGQKLMSYVQTLEKDSPKIKENYDKLLDFKNHIRMER
                     LTRQVVPYLEKYSGNEFIRSEYREHLEQSEENGSYASQEGKVFEAYVDSAEQAFDRIV
                     NRVNELESKNLQKKVPDTAPKVAEKPKLDFKVQTRTTYQFLDFKTKEQEDPNLPLGEK
                     VERQKGERGEESETYQDVIVGDKIVASTLISKKRKDPVDHLIAKGTLVEKHPDTAPTA
                     PEKPKLDFTVKERVEREILDYMVEEQDDASLPLGQTKVLREGQKGELVKKYQDVIVEG
                     KVIESKLLSEDRKTPVNRLIAKNILVEKHPDTAPTAPEKPKLDFTVKERVEREILDYM
                     VEEQDDASLPLGQTKVLREGQKGELVKKYQDVIVEGNVIASNLLSEDRKAPVNRLIAK
                     GTLVEKTPDAPAPKAEEEVPASTPMPETPMDKPKTDKVESDKQMPEAKQPEMEQPKAE
                     DMPKAEQPKAEDSAPKTAVPEVAPKTAEKPKLDFTTKERKVEEALPIKEEIRYDASLA
                     LGKSYLLQEGKAGKKVSVYQDVIVDGKIVATNLLSETVVEGQNRILVKGSLEMKKEEV
                     KSTPSVQSNLTLSHKGAPSANKATLPATGEQRNNLALVGLGLAGISLAVVATAINKKS
                     KDQI"
     gene            complement(11052..12221)
                     /locus_tag="AC1PNG_000008"
     CDS             complement(11052..12221)
                     /locus_tag="AC1PNG_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIP6700554.1"
                     /translation="MFRLAKEELEKLGAMITATEIYQQPGLWKEAHQLYFDQLEKIES
                     FLKSIKEKHDFVHVVFTGAGTSDFVGQSIANYLNQVNDLKHIRFSAIGTVELVSRPHD
                     YLQADLPTILVSFARSGNSPESVAAVEVAKQLVDKLYQVTITCAPEGKLALAAEGDVE
                     NLLLLQPAGSNDKGFAMTGSYSCMALTALLVFSPASQEEKAAWVEIIARLGQDVLDRE
                     DYVQDLINLDFERVIYLGSGGFYGLAHEAQLKILELTAGKIATMYESPLGFRHGPKSF
                     INEKTLIFLFASNDAYTRQYDVDLLNEVHGDGIAERIVALSADKLVGTEAANFVLAEG
                     GADLPDVFLTFPYILFAQTVSIMAAIKCNNLPDTPSPTGTVNRVVQGVIIHPLEK"
     gene            12500..12799
                     /locus_tag="AC1PNG_000009"
     CDS             12500..12799
                     /locus_tag="AC1PNG_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIP6700555.1"
                     /translation="MMNMQNMMRQAQKLQKQMEKSQAELAAAQFTGSSVQDLVTATFT
                     GDKKLVSIDFKADVVDADDIETLQEMTVQAVNAALTKVDEATQKKLGAFAGKLPF"
     gene            complement(12839..14599)
                     /locus_tag="AC1PNG_000010"
     CDS             complement(12839..14599)
                     /locus_tag="AC1PNG_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIP6700556.1"
                     /translation="MKKIQREFQKIYYNLDKILLLFFTLFTFMEFVWIPLNSWISEGL
                     LAMTGHAYLSPTNLLSVFAENLLVTGLFILLFFVNIAIAYLELALLFTGVWQLLDEKV
                     KHLPDYLRDVRDSMVAIIRHSSLPKVLFLLFYSVILLPFLRRVLNIYYFNKIVVPQFI
                     VDYLSNTVWLGILILIFLLLFFWLAARFMYALPNIYFEHRGVKESIAYSWEKTRGRKQ
                     IGSLFRLIWLITFPVLLFTGVGGLFYLVQLLAEHFVPSASYWLAVVCYALLKLAYYGV
                     VALFMMGFVSLLTGKQLPICRRKRLRHHLRLAILLVSGLVFGTQGALALYFPFDTLPV
                     TISHRGVDDGNAVQNSIEALEKTSQLKPDYIEMDVQETKDGQFIVMHDTDLLALTGNP
                     GGTHDYTLAELTGMTASENGMVAPVPSFDEYLEKADELGQKLLVEIKVTKADSPKLTQ
                     NFLKKYGQRLIAKGHQMQSLDYRTIIEIKQYSEKLVSFFILPFNSIYPTTVADGYTME
                     YTSLDQNFMTKSWIRGKSVYAWTPNEEESMTKMLLLQVDGIITDNMTDLQSLMEEMKE
                     NRRYPDLFFQQFQSLIYNFE"
     gene            complement(14790..15716)
                     /locus_tag="AC1PNG_000011"
     CDS             complement(14790..15716)
                     /locus_tag="AC1PNG_000011"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIP6700557.1"
                     /translation="MRQTKEMRLVYSDFLAQVGQEDTTIAAIEADLSSSMATNKLSSV
                     LGGRYVNVGIMEQEMVGVAAGLSLLGYKPYIHTFGPFASRRVYDQVFISLAYAQLNAT
                     VIGSDAGVSAEMNGGTHMPFEELGLMRLIPKARVYEVSDDVQFQAVLKETIKVDGLKY
                     IRTIRKATTAIYQGGEDFSKGYCVLRQGEDMTLLASGIMVEQALKAADQLEAQGVSVQ
                     VIDLFRIKPIHEDIPALLSGRPVLTVENHNRIGGLGSSICELMATDLTTPVHRIGIEE
                     SFGQVGQQAYLMDVYGLTAEYIVKQAQLMLQQ"
     gene            complement(15713..16561)
                     /locus_tag="AC1PNG_000012"
     CDS             complement(15713..16561)
                     /locus_tag="AC1PNG_000012"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIP6700558.1"
                     /translation="MTSGTADLKRFAKEIRYHTLATLNQLGFGHYGGSLSIVEVLAAL
                     YGEILDIRVQNFSSKERDHFILSKGHAGPALYSTLYLKGFFDRDFLLSLNQNGTHLPS
                     HPDRNLTPGVDMTTGSLGQGMSVATGVAYGKKIEQSPYYTYTLVGDGELNEGQCWEAA
                     QFAAHHELSKMILFVDDNKKQLDGRTHDICQTFDFVEKFQAFGWESVRVNGADIDAII
                     NAILTMKSSKSPQPKCIVLDTTKGQGVTFLEELESNHHLRLSGQLREDLEQVTLVLAR
                     ELEVME"
     gene            complement(16563..17909)
                     /locus_tag="AC1PNG_000013"
     CDS             complement(16563..17909)
                     /locus_tag="AC1PNG_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HIP6700559.1"
                     /translation="MVLDFLIDIAKTPAILVALIAILGLALQKKPAADLIKGGLKTFV
                     GFIVVGGGANIIVGSLEPFGQMFEQAFNLKGVVPNNEAIVAMALDKYGTATSLIMLLG
                     MVFNIAIARFTRFKYIFLTGHHTLYMACMIAVIMSVAGFTSVGLIVMGGLALGLIMTL
                     SPAFVQKYMIQLTGNDHVALGHFSALGYWLSGVVGGLVGDKSKSTEDINFPKGLAFLR
                     DSTVSIAISMSLIYMIVALFAGGDYIKENLSGGTNSMIYALTLGGTFAAGVFVILSGV
                     RLILAEIVPAFKGISEKLVPNSKPALDCPVVYPYAPNAVLIGFISSFAGGIVSMIILA
                     LTGGVVILPGVVPHFFCGATAGVMGNASGGVRGAVCGAFMQGVLISFLPVFLLPVLGD
                     LGFAGSTFSDADFGLSGIFLGVLAKNGGILTVSVGIFAVLALMLGLSLVKKEQKEG"
     gene            complement(17963..18247)
                     /locus_tag="AC1PNG_000014"
     CDS             complement(17963..18247)
                     /locus_tag="AC1PNG_000014"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIP6700560.1"
                     /translation="MLRIGTACGSGLGSSFMVQMNIESILRDLGVSDVHVEHYDLGGA
                     NPSEADVWIVGRDLADSATHLGDVRILNSIIDMNELRELVTAICQEKGLV"
     gene            complement(18249..20291)
                     /locus_tag="AC1PNG_000015"
     CDS             complement(18249..20291)
                     /locus_tag="AC1PNG_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIP6700561.1"
                     /translation="MVDNRTMAILMELFATRKESLYELSIQTGIEKEQLHSNLELVNQ
                     LLQEHSFPQIQYKDSEFVISTELYNQKESVFSLFRNRQIYLSQEERQLLIYLYTFIRK
                     EFVSNVHYQELLSVSRNTTLTDIKNVKELCLDFQVRLEYTRAKGYHLIGREEDKHRLA
                     LYALSNCLQSSIGVWALDYILKSWGEENPIEELKIVSQQACNYYKVSALEERLDEYLY
                     FLIFLFIRQARVGNRINWNFEGKGGFVKDFLQRLWKLLRLKSTSTLELNAATQEYLSH
                     LLQGCLEGESGEKDDLFYHLTVEIVEEMERLSLIAFEHRSEMIEGLQRHLIPAYYRLT
                     SRLVNVNSYTETIKEEHADLFYLVKKALRPLEEHLGFTIPDSEVSYFVIHFGGYIEAG
                     QQRSYRYRALVVCPNGVSSSLIVKENLRQLFPNIYFADTHSLQDWKMLDMTDYDMVFG
                     TIKLNIELPFFLVSQLMTSNHKKELFHLVNQHFPNAAYFPIEIEQLLSLVGKHATIHQ
                     EQALKYELVQFLNQRSHEQRGRSPMLEELITKETFQWSEEVLDWQKAVALVARPLVDK
                     GAIETRYIDAMIAKVEEFGPFIDLGKGIAIPHARPEDGVNEVGMSMLVLDKPVYLLDD
                     PSHEIRLFICIAAVDNQTHLRALSHLTKILRAEENIQQLVGAKEFADVQHLLKEEQ"
     gene            complement(20612..21106)
                     /locus_tag="AC1PNG_000016"
     CDS             complement(20612..21106)
                     /locus_tag="AC1PNG_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019783378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HIP6700562.1"
                     /translation="MGIEKTVSELAEILGVSRQAMNNRVKSLPEEFVDKNDKGVTVVN
                     RAGLIKLEEIYKTTIFEDEPISDEVKHRELMEILVDEKNAEIIRLYSQLKAKDKQLAE
                     KDEQLKVKDVQIAEKDKQLDQQQQLTLKAMADKEVLKLELDEVKAQAEEVQAKGFFAR
                     LFGK"
     gene            complement(21276..22676)
                     /locus_tag="AC1PNG_000017"
     CDS             complement(21276..22676)
                     /locus_tag="AC1PNG_000017"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIP6700563.1"
                     /translation="MVTEQREYRFPEGFLWGSSTSGPQSEGSVAGDGKGWNNWDYWYS
                     QEPELFHQQIGLEQTSTFYEHFQSDLDLLVETGHSIFRTSIQWSRLIPEGIGEVNPQA
                     VEFYRAVFAGIRERGIKLLVNLYHFDLPYVLQEKGGWENKEIVWAYEAYARQCFSLFG
                     DLVDHWITFNEPIVPVECGYLGSYHYPCKVDAKAAVVVAYHTQLASSLAVRACHQLHP
                     YHKISIVLNLTPAYPRSQSPEDLQAARIAELFQTRSFLDPSVLGQYPRELVDILEKHG
                     LRPETTEEELTIIKENRVDFLGVNYYQPLRVQAPSEDGWNGEATFLSQYFQPYDKPDK
                     KINPHRGWEIYEEGLYDIAKNIQENYGNIEWLVTENGMGVEGEDVFEENGMIQDDYQI
                     HFMEDHLIQLHRGIDEGANCKGYMVWTFIDCWSWLNTYKNRYGLVSLDLQTQKRTVKK
                     SGYWFKQVSAKNGFIR"
     gene            22914..24089
                     /locus_tag="AC1PNG_000018"
     CDS             22914..24089
                     /locus_tag="AC1PNG_000018"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIP6700564.1"
                     /translation="MNTVDTIIIGAGPAGMMAAISSSFYGKKTLLLEKNKRLGKKLSG
                     TGGGRCNVTNNGTLEDLLAGIPGNGRFLYSVFSQFDNHDIMNFFQENGVKLKVEDHGR
                     VFPTTDRSQTIIKCLEMKMLENGVNIRTGTEVVSVRKIDDLFHVKTSEETFTAPQLIV
                     TTGGKTYPSTGSTGFGHDIARHFKLEVTEIEAAESPVLTDFPHKKLQGISLDDVTLTY
                     GKHVITHDLLFTHFGLSGPAALRLSSFVKGGETAFLDALPTHSDQDLFEHLEANREKS
                     VKNALRELMPDRLADFFAENYDCKVKQVSQKDLTDLVSLLKALPIKITGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVPGLHFAGEVLDINAHTGGFNITYCLATGWVAGSSYSS"
     gene            24330..25022
                     /locus_tag="AC1PNG_000019"
     CDS             24330..25022
                     /locus_tag="AC1PNG_000019"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6700565.1"
                     /translation="MPINSFGQKIGMPLPHYSSGERPSLETIQGHTVRLEKLSLHHAD
                     DLYQFYGPSAKKTDWTYLPLEPFEDRATFQDYFQTLLNSTDPYYFAILDQATNQAVGT
                     FALMRIDTNHRTIEVGWVIFSPQLQRTRQATEAHYLLMTYVFEELGYRRYEWKCDSLN
                     QASRVAANRLGFTYEGNFRQALVYKERNRDTAWFSLLDHEWPDRKRALENWLKDSNFD
                     GQGQQKQPLSSF"
     gene            25103..25801
                     /locus_tag="AC1PNG_000020"
     CDS             25103..25801
                     /locus_tag="AC1PNG_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP6700566.1"
                     /translation="MQLKLSSVSKKFDHKIILEDTSFTFEQGKIYGLLGRNGAGKTTL
                     FNCIARNLTLDGGSIQFVKDEIAFDYDNTDIGFTQTYPQLPAFMTAYEFVRFYMDIHK
                     DKLKSPRSPEEWLNLVGIGEEDRHRLLKDFSHGMQNKVQLLLSLIVQPPVLLLDEPLT
                     SFDPVAAHEFKQLIREAKKDSVIIFSTHILQLAQDLCDEIVLLHHQELQAVPSDKLHD
                     PDFEQEVVTLLTAD"
     gene            25811..27436
                     /locus_tag="AC1PNG_000021"
     CDS             25811..27436
                     /locus_tag="AC1PNG_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700567.1"
                     /translation="MKTILRYFWYQEKYNLYRTVNGFFYYLRKLPLVGKSIPESIFKS
                     YSFKSGLFLILHVLSIPSRFLVKGIWLALNFYLASFWINLLSGGDLTFWNILPGTWLL
                     GFSLWMVFVGYTYRFSKGFEPFIVKSEREFMQNFGLSQSSFLQSQLFVEPIITSLFYL
                     PALLIFSGLSGNWLYLPLGLLTIPTGSFTGQALNRWFFNRRILSRRNSWQSWVILGTG
                     LAAIACLILFRNHLSPIFLLPVLVCQVLLIWFGYGYLKQQTNHLDYLYYCMDQSLQMD
                     KKIFEMTKGNEYTRQGLQMQAKLSMETGKDLTHLSGMTYLNALLFDRYRSVLTKKLHF
                     RLGCLLAIVLFIEGIRWFSKDDIQISNTNYIEGLPFAFMVMYAASMGKAVAQMVFVNC
                     DISMLHYPFYREAKTIIAGFHYRFLQTVKLNAAFSGSLLLALIIFTRAQLPIETYLLT
                     ALLLLSLTALFSFHDLFIYYILQPFTKDMEVVNPVYKFLSGALYWVAYLNIKLDLGSH
                     MYILLISIAMITYVSIGYWILLKKAPQTFRLKS"
     gene            complement(27736..28758)
                     /locus_tag="AC1PNG_000022"
     CDS             complement(27736..28758)
                     /locus_tag="AC1PNG_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HIP6700568.1"
                     /translation="MVKENAKGVGLCFLLALVGQWLGHLFPLVGGPVFALLIGMSLYP
                     YFSSKNAFQSGLTFTSKKILQYAVICLGFGLNLSAVLAVGRQSLPIIFSTISFALILA
                     FLMWKILPISSHLATLIGVGTSICGGSAIAATAPIIQADDEDVAQAISVIFLFNVLAA
                     LVFPTLATWLGFSTDSGQVFGMFAGTAVNDTSSVTAAASTWDSLYGLGSQTLDTAVTV
                     KLTRTLAIIPITTVLAIWQTRGKGVQADKKSLLAGFPVFILYFILASLITTIAGGFGI
                     GADWFAPLKSLSKVFICMAMVAIGLRTNVFALVKNGRAALLVGLVCWFGVTALTLVWQ
                     AILGIW"
     gene            complement(28768..29091)
                     /locus_tag="AC1PNG_000023"
     CDS             complement(28768..29091)
                     /locus_tag="AC1PNG_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIP6700569.1"
                     /translation="MIKTNILLIILMAALVTWVPRVLPFVLTQNKSLPPKLVKFLSFL
                     PITIIFALTLSSIMDEKVGSLPSFLPVESLAILPTFFVVLKTKNILLAVLVGVVTTAL
                     LRLIF"
     gene            complement(29078..29779)
                     /locus_tag="AC1PNG_000024"
     CDS             complement(29078..29779)
                     /locus_tag="AC1PNG_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIP6700570.1"
                     /translation="MKKYAFREGMRDAIPTVLGYASIGLACGVVSVNSGISTVEMGLM
                     SLLIYAGSAQFVMCAMIFAGAPLLSIAVTVFFVNLRNFLMCLHATTIFQGNRLGSNIL
                     IGSFVTDESYAVLLRKQIEHHTITPEWMYGNNFASYASWVTFTTLGNLIGGLIPNPEQ
                     FGLDFALVAMFVGIFAGQLEAMVRTIPLKKIGLILLAVGLTYIGLSMMVSSYVAVLLA
                     TLLGCFVGVMIDDKN"
     gene            complement(30054..32255)
                     /locus_tag="AC1PNG_000025"
     CDS             complement(30054..32255)
                     /locus_tag="AC1PNG_000025"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004557 - alpha-galactosidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HIP6700571.1"
                     /translation="MGIIFHEKTKEFHLYNEFISYVLTVLPNGHIGSLYYGKRVTETA
                     SYQYVREDEYRALTSFVEDDDRFFSLQYANPEFACYGTTDYFSPTFELVQKDGSSLSH
                     FVYQGHVIYAGRSALKGLPHLYLDEEGQADSLDIYLLDEESQTRLVLSYTIFRDYPAV
                     TRSARFEQLGEQSVCLNRALSMTLCLPDMDYDWLHLDGAWGRERHLQLSPLHQGCQSI
                     YSLKGASSAEHNPFMALKRPTADEQQGEVLGFSLVYSGNFLAQVDVSSFQKTRVSMGI
                     HPERFSWTLDKGEEFQTPEVVMVYSEHGLNGMSQTYHRLFQKHLVRGYWRDRERPVLL
                     NNWEAMSFDFDEETILSLAKEAAGLGVELFVMDDGWFGKRNHDRAGLGDWTVNREKLP
                     SGLTGIIDQVHAMGMKFGLWIEPEMVNKDSDLYRAHPDWIFHHPQHSQSHGRHQYTLD
                     LSREDVYQNIHDQLHRLLAEHDIDYIKWDMNRYMTEVFTCTREAERQGETFHRYILNL
                     YRLYDSLIAAFPTVLFESCSSGGARFDPGMLYYAPQTWVSDDTDAMERLKIQYGTSMV
                     YPLNSTGCHVSASPNQQLGRATPLSTRANVAFFGSFGYELDLFECSQEELEEMKEQIA
                     FYKAHRKLFQQGIFTRLKSPFDGEITAWQVQSLDGQQVIVGYYRRLTTANLSYQRLYL
                     QGLEEEAVYHLDGEVYTGSQLMHTGLSIRHGDHVGDNKDFTSYLMVLQKQQ"
     gene            complement(32265..33095)
                     /locus_tag="AC1PNG_000026"
     CDS             complement(32265..33095)
                     /locus_tag="AC1PNG_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIP6700572.1"
                     /translation="MKQSAFKSTIWMHAFLILVAMGMLIPFVWMVLTSFKTVTESTQM
                     NPFHFLPSNWMVSNYTEAIRTNNFPILYLNTILMMLWRIFSSVMFSAMAAYAFARLEF
                     PGRNFLFGLVLFQMMVPPQLFVIPQYLMIDQLGMRNTIFALVFPGIVSAFGTFLLRQF
                     FMGLPKELEESAKLDGCNIGQTFFKVMLPLAKSGLIALAIFTALFAFKDLLWPLIINS
                     EADKATLSSALSKIQGAYAVNYPQLMAASVLAIWPMLVLYVVFQKQFIQGIATSGGKL
                     "
     gene            complement(33106..33999)
                     /locus_tag="AC1PNG_000027"
     CDS             complement(33106..33999)
                     /locus_tag="AC1PNG_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIP6700573.1"
                     /translation="MFRKKGSLNEAIWGWGMVAPTIIGLIVLNIIPIFQTMKMSFHKS
                     GDFGKGDIFVGLANYQRMLGDAQVWQATWNTLKYTILVVPATVALAMLLAVLLNSKIK
                     GKHIYRTIFFLPMVAAPAAVTMVWKWLYNTDFGLINYILRRLGLGAVNWIEDPKIALY
                     SIALIGIWSTVGYSMILILAGLQEIPTDFYEAARIDGASPAKQFFSITLPLVSPTLFF
                     VVVTSVIQSMQVFDVIYMMEDVRSPAYDKTVSLVYLFYNNSFKYSDKGYGSTIVMLLL
                     LIILVITFVQMKVQKKWVHYR"
     gene            complement(34068..35342)
                     /locus_tag="AC1PNG_000028"
     CDS             complement(34068..35342)
                     /locus_tag="AC1PNG_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP6700574.1"
                     /translation="MKKIGRYVTLLAAAGLLAACSTSTESSKTTEGSSSGKTEISYAI
                     WDSGQEPGLRKIADEFEKKNPDIKINIQVSDWDSYWTMLEAGATGGSLPDTFWMHSNE
                     IYRYGSNEMLLPLDEYLAKSEDAKLANFPDGLNEIYNINGKQYAIPKDFDTIGLWYNK
                     KLFDEAGIPYPDDTWDWNKLKEVAKKLTKPDGSQYGFGAGLSNQEGYYNFIYQNGGKV
                     ITDDLKSGYADPKTIEALDYYFSFVKEKISPAITVDKERAEAFQNGQVAMSVFGSWNL
                     SGFTANDYIRENADVAVLPKGPDGTRATIFNGLGHAIAATTKHPDAAWKWVEYLSSKE
                     AQEMQATLGVAISAYKGAADTWVDSNKNFTIKNYVDMVDYAQIRPYSQTTIKWEDKAY
                     ELLKPAYLGEKATEEAAKETADMMNAELATEK"
     gene            35557..36393
                     /locus_tag="AC1PNG_000029"
     CDS             35557..36393
                     /locus_tag="AC1PNG_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIP6700575.1"
                     /translation="MKIQPEFYVFNNRSYVDFYPIQFGMEACKPLHSFGPTMKQHYLF
                     HYIISGSGTFYDTSDQREYHLTAGQGFLISPDAICSYEADEKDPWTYIWLEFDGLKTE
                     HFLRQAGLSKEQPIFSQREMPTSSPVYHEMKQILALHHQKSALVLGHLYLFISCLIDC
                     SLTKTSSKHDENKEFYIREAINFVERNYEKAISVDDLAQVCNLNRHYFSRLFKEQMNI
                     SPQQFIIQYRLSEACELLKNTTKTLQEIAEEIGYSNQFNFSTAFKRHYQISPNRWRKI
                     HK"
     gene            complement(36430..37437)
                     /gene="tsaD"
                     /locus_tag="AC1PNG_000030"
     CDS             complement(36430..37437)
                     /gene="tsaD"
                     /locus_tag="AC1PNG_000030"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIP6700576.1"
                     /translation="MKDRLILAIETSCDETSVAVLRNDAELLSNVIASQIASHQRFGG
                     VVPEVASRHHVEVITACIEEALLEAEVTAEDLTAVAVTYGPGLVGALLVGISAAKAFA
                     WANGLPLIPVNHMAGHLMAARAVKELEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLPYPAGRVIDELAHEGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAQQKGETLSNADLSASFQACVMDILMAKTKKALEQYPVKTLVVAGGVAANQ
                     GLRERLAAEITDVEVIIPPLRLCGDNAGMIALAAVSEYNKENLAGWDLNAKPSLAFEN
                     L"
     gene            complement(37427..37867)
                     /gene="rimI"
                     /locus_tag="AC1PNG_000031"
     CDS             complement(37427..37867)
                     /gene="rimI"
                     /locus_tag="AC1PNG_000031"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIP6700577.1"
                     /translation="MIEIKYYDGQENLSEAVLAVMESVYEQSPWTLEQIVTSLASQDE
                     DYYLAYEGQELVGFLAVQTVLDEMEILQIAVKADFQRLGIASQLMAAVMDWEGDIFLE
                     VRESNSAAQALYTRQHFTKIGKRKDYYRHPVEDAVLMKRERDER"
     gene            complement(37864..38547)
                     /gene="tsaB"
                     /locus_tag="AC1PNG_000032"
     CDS             complement(37864..38547)
                     /gene="tsaB"
                     /locus_tag="AC1PNG_000032"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIP6700578.1"
                     /translation="MKILALDSSNQALSVALVEDGRLQAETLLAVKKNHSISLMPVVD
                     FLVAQVGWTPKDLDRIVVAEGPGSYTGLRVAVATAKTLAYTLKIDLVGLSSLQSLVPP
                     SLTGLVVPLIDARRNCVYAGLYENGRAVEADKYWSFEDLLSSLSGKEDITFVGEVANF
                     IEQIEQALPTAQYQASLPSAYQLAVIGQDLPAVDVTSFEPNYLKRVEAEENWLKDNQA
                     GSESYIKRV"
BASE COUNT        11280 a         9364 c         7430 g        10859 t
ORIGIN      
        1 tgaaaatacc aactctatct ttcaattttt tcatattcct tgaaaggttt ttcatttatt
       61 ttcaagtatt tttgtcttgt agaaagtgtt ttcaaatggt ataataatga catgttggta
      121 tcgatagcaa gtcgataccc aaaagcataa aaggagaacc ctcatgtcaa caaaagttaa
      181 aacaaaaaat gttgctgaag acattttcgc ccaagcctgg gaaggcttta aaggtacaga
      241 ttggcaagat aaggcaagtg taacacgctt cgttcaagcc aactacactc catacgatgg
      301 agatgaaagc ttcctcgcag gcccaactga gcgttcactt catatcaaaa aaatcatcga
      361 agaaacaaaa gctggctacg aagacactcg cttcccaatg gacgtagatc gtgctacttc
      421 tatcgctgat attccagcag gtttcatcga taaagagaac gaactcatct tcggtatcca
      481 aaacgatgag ctcttcaaac tcaatttcat gccacgtggt ggtattcgta tggctgaaac
      541 aactcttatc gaaaacggct atactccaga cccactcctt catgaaatct atacaaaaca
      601 cgctacaact gtaaatgacg gtatcttccg tgcctacaca gctgacatcc gccgttctcg
      661 ccactcacac cacgtttctg gtcttccaga tgcatactca cgtggtcgta tcatcggtat
      721 gtacgctcgt ttggcacttt acggtgcaga ctacttgatg gaagaaaaag tagctgactg
      781 gaatgcgatt actgaaatcg acgaagaatc aatccgtctt cgcgaagaaa tcaacatgca
      841 gtaccaagca ttgcaacaag ttgtacgctt aggtgatcac tatggtgttg atgttcgcaa
      901 acctgcaatg aacactaaag aagctatcca atggacaaac attgctttca tggctgtatg
      961 tcgtgttatc aacggtgcag caacttctct tggtcgtgtg ccgatcgttc ttgacatcta
     1021 tgcagagcgt gacttagctc gtggtacatt tactgaatct gaaatccaag aatttgttga
     1081 tgacttcgta ttgaaacttc gtacagtgaa attcgctcgt acaaaagcct tcgacgaaat
     1141 ctactctggt gacccaacat tcttgacaac atctatggca ggtatgggta acgatggtcg
     1201 tcaccgtgtt actaagatgg actaccgttt cttgaacaca cttgacaaca tcggtaactc
     1261 tccagaacca aacttgacag ttctttggtc agaccaactt ccatattcat tccgtcgcta
     1321 ctgtatggca atgagccaca aacactcttc tatccaatac gaaggtgtga caacaatggc
     1381 gaaagatggt tatggcgaaa tgtcatgtat ctcttgctgt gtatctccac ttgatccaga
     1441 aagcgaagac caacgccaca acattcagta cttcggtgct cgcgtaaacg tccttaaagc
     1501 tcttctttca agctggaaca acggttacga tgacgttcac aaagattaca aagtattcga
     1561 cggtgttgaa ccaaacacat ctgaagtatt tgactacgac caagtagtta agaactttga
     1621 aatcgccctt gactggttga ctgatacata tgttgatgcc atgaatatca tccactacat
     1681 gacagataaa tacaactacg aagcagttca aatggccttc ttgccaactc accttcgtgc
     1741 taacatgggc ttcggtatct gtggtttcgc aaacactgtt gactcattgt cagcaatcaa
     1801 atatgcacaa gttaaaccaa tccgtgacga agatggcttc atctacgact acgaagtaac
     1861 tggtgacttc ccacgctacg gtgaagacga tgaccgtgta gatgatattg cgaaatggct
     1921 catggaagca ttcttcactc gtttgaacaa acataaattg tacaagaatg ctgaagctac
     1981 agtatctatc ttgacaatca cttctaacgt tgcttactct aaacaaacag gtaactctcc
     2041 agttcaccgt ggtgtattcc tcaatgaaga tggttctgtg aacactacta aggtagaatt
     2101 cttcccacca ggtgctaacc caacttctaa agctcgtggt ggttggttgc aaaacttgaa
     2161 taccctttct aaactcaact tcaaacatgc caacgacggt atctcattga ctactcaagt
     2221 ttctccaaaa gcgcttggca aaacattcga tgagcaagta aacaacttgg taacaatcct
     2281 tgatggttac tttgaacaag gtggtcaaca cgttaacttg aacgttatgg acttgaacga
     2341 cgtttatgaa aaaatcatgg ctggtgaaga tgttatcgtt cgtatctctg gatactgcgt
     2401 aaacactaaa tacctcacta aagagcaaaa aactgaattg actcaacgtg tcttccacga
     2461 agttctttca atggacgacc acgctgctga agtttcaggt caagcttaat caagcaaaca
     2521 caaaaggact tgcaatgcag gtccttttta ctatgtcgaa aaatctactg agtgttgcgg
     2581 aaggatagta aatcttcgga aggatgcacc gcagattttc ggaaggatac agaaccagtt
     2641 cataaaaaaa tccctttcga aattggattc gaaaagggat gattatttat cctagttatt
     2701 atagatatag gtaatttctc cgatggtatc cgtcggatta aaccagccgc ggaaattatc
     2761 cacccactgt tggccacgat agttggcttc gaggacttga atttgtccgt cctcgttaac
     2821 agctgtcaca taggcaacgt gtcccagagc accatcattc caagaaataa tggctccaac
     2881 ttcaggtgtt gttcctgtac ggaaaccatc acgcgccgca ttggctcccc aagaagccgc
     2941 atcgccccac cagtcacctg cccaaggggc aactttctgt actccccatg tacattgacc
     3001 aacagggtag ttaatgcctg gttcatactc atagcctggt aaatagtaac tgtcagcagc
     3061 cgtatcaacc gtctgattcc aaattgtttc tgttaacttg agaatttgac ctggatgaat
     3121 attggcatct aatgtcaact gattttgttc cagcaattta ttgatgtcca atgaaaaagc
     3181 atctgcaatc ccgtaaaacg aatccccatc ttgaacaaca tagtctgttt gacccgcaac
     3241 tgagactcct tcattagaac cagctggaag atccaaacgt tgacctggtg taatcagtcc
     3301 gtctatttcc atcgcattca cttcagccaa ttggtaggga tcaaccccat attgctccgc
     3361 aatagagaac atggaatccc ctgccattac tacatgacta gtagcttgaa cacgagatat
     3421 ggttgataac gcaaccgtaa tccctgcaag agctagaaca gatttgcaac taaacttttt
     3481 catatgacac ctcctcatca tgatttcatt ttaccaagat tttgacggga aaagatgtcg
     3541 aaagcattac aaataaatga cgatttatcg ttttatttca ttctctaaat agtttgatat
     3601 aactgagaaa gtcttcccat tccgaatggt cacttgtttg taaaaaggtg acttagccaa
     3661 ctgcttgtgg taggctgttt gtagatagct accttcagtg tatttgcccc aataaatagc
     3721 atagtcggag aaatgtagaa cttcatcacc tagatgcaag gactgaatat agtccaccat
     3781 agcctgctta tccaatcctt cggtgaaaaa gagaacatcc tttctcgcca attcttcttg
     3841 ccaccaagct ggaagctgat cgatttctgc ttgataatct tgggtgtcta gtagagcaaa
     3901 ttgctccaca aaggggtatc tttctgtgaa aaaagtttga aattcagcaa caatgtcccc
     3961 tctattcttg gttgaatcaa agaagagatt accgctatta atgtaggttt caaccttgtc
     4021 atagcccaaa tctgccaccg cttgacgcaa ttcagccatg acgattttgt tacgaccacc
     4081 gacattgatc cctctcagca gaagaacata tctcattgcc cctcttcctc caagacctga
     4141 cgaatcaagg ccataacctg gtttctatcc aacttccact gggcccgctc atccttgtca
     4201 aaagtacgat tcgagagaaa aatcgcagct ttctgcaagg gacggttata catgataaag
     4261 gtccccgtat agcctgtgtg cgacaaccag tcccccttct tatcccaagc cagagaccgc
     4321 ttacgcttaa catcaaaacc gtaatcctgc gttaaattcg ccgcaaagtc agccatcaga
     4381 tagtgttcca aaaagatttc caaatctttg acggtcgaaa agagacctgc acttcccgcg
     4441 tggaggccca agacacgcgc cttgggatca tgcaccacac ctgctttgac gccacgaacc
     4501 gtcggcaccg cctgactgac tggtccaaag gaggtttggg acataccaaa gggctctaga
     4561 acgtctcgac taatcagctg gtctagactg tctctaccca attcctccaa caaaaagccc
     4621 agtaagagaa aattaatatc cgtgtagagg aaagctttct tatccgttac agcaatcttc
     4681 aaaatcgctt ccttgagctg gtcggcatcc agactatccc tatttggaat aaagggatca
     4741 ataccactag tatgggtcaa taattgccga atggtgacct cactatccgc aaaagctgga
     4801 taataagact gcaaggtcct atctagctcc aaagctccgc tattaaccag aaaagtcgca
     4861 agagtcccca caccaaccac cttggaaaca gacgccaaat cataggtcaa acccgcctca
     4921 actggcgtat gcccatcctg agtccctaga tagtactcct gccactgacc ctcagaaaaa
     4981 aaagccagac tggcaccagg ataaactcct tgctcaatct gctctgaaac cttgtctaaa
     5041 attactcgtc tcatttttcg aaccacaatt caataccact tgggtcaact gtttggataa
     5101 agcttgcttt tttatcttta aagaatggtc cttccaaccg gctttccaaa tgtgcccaat
     5161 caaagtcctg ctcgacggga aaacgcaatc cttctaaatc ccaaacttgt tctgcaggag
     5221 ttaataaatc ctcaccttct gctccaataa aacgcaaaaa ggcttgttga ggtaggatag
     5281 cctcataata gtcttggtta atactagctt gtggggtatt aatccagaca gactctactg
     5341 taaaagcagt caatccagaa aagtcatcct gcaccttaaa tggaacaggt ggaaggattg
     5401 cttttaggtc atcaacagac gcttctgcgt gtaaaaggaa ggtgtctcct tctggcgaca
     5461 cagcttcaaa accatagcca ttttttcctt ggtagagctt actatactgt gacccttgcg
     5521 ccagtaaagc ttcaatttca gaggcattat ctacttttat cacaatttta ttcaattttt
     5581 taagcccttc gacagcacgg gtgcgattac caggagattc tgtcaaaacg agcttcgttt
     5641 ctggatttgt tctatctcca aaatcagcaa aaggaccatc ttctagcttg gttttcattc
     5701 ctagattttt ttctaaaaat ccttgattaa tagccctatt attaatacgt aaaacaggga
     5761 ctatagcttc attttttctc aacacgatac atttcactcc tcagtcattc tacaatattg
     5821 tataaaaaat ttcgtcattt tacaactaat tatatcgtcg ttttatacat taatatcttt
     5881 atatatactg ataattttta gacaaacatt gacttatccg atataatagc tattatagac
     5941 tattatccta aaaaagagga gaatttatgc gctttaatca attttctttc ataaaaaaag
     6001 agacctccgt ctacttgcaa gaacttgatg ctctaggatt tcaactgatt cctaatgtaa
     6061 gtagtaagac aaatttagaa acctttgtca gaaagtgcca tttcctgaca gcaaacaccg
     6121 actttgccct gtcaaacatg atcgcagatt gggacacaga cctgctgacc ttcttccagt
     6181 ctgaccgaga gctgacagac ctgattttct atcaagtggc ctttcagttg ctaggcttcg
     6241 tgcctggtgt agattataca gatgtcatgg atttcgtgga gaaaagcaat ttccctgtca
     6301 tctatggaga tatgattgac aacctctatc aactgctggc tactcgtacc aagtctggaa
     6361 acaccttgat tgaccagctg gtcagcgatg acctaatccc agaagacaat cgctaccact
     6421 tcttcaacgg caaatctttg gcgacctttt caaccaaaaa ccttattcgt gaggtggtct
     6481 atgtcgaaac gcctgtcgat acagctggga ctgggcagac agacattgtt aagctgtcca
     6541 ttctccgtcc gcactttgac ggaaaaattc ctgcggtgat tacaaacagt ccttatcatc
     6601 agggagttaa cgatgtagcc agcgacaagg ccctgcataa gatggaaggg gaactggctg
     6661 aaaaacaagt tggtaccatt caggtcaagc aggccagcat caccaagtta gacttggacc
     6721 agcgcgagct ccctgtcagc ccttccaccg aaaaactggg ccacatcact tcatactccc
     6781 tcaatgacta ttttcttgct cgtggtttcg ccagcctcca tgtgtctggt gtcggtacgc
     6841 tgggctcgac gggctacatg acatctggcg actaccaaca ggtggagggc tataaagcgg
     6901 tgattgactg gctgaacggt cgcaccaagg cctacacaga ccacacccgc tcgattgagg
     6961 tcaaggccga ttgggccaat ggtaaggtgg cgacgacggg actgtcttac ctcggcacca
     7021 tgtccaatgc cttggcaaca actggcgtgg acggtttgga agttgtcatc gcagaagcag
     7081 ggatttcctc ttggtatgac tactaccgtg aaaacgggct ggtgaccagc cctggcggct
     7141 atccgggcga ggacctggac agcttgactg ccctgaccta ctcgaaaagt ctgcaagcag
     7201 gggatttcct ccgcagcaaa gaagcctacg aaaaaggcct ggctgccgaa cgagctgccc
     7261 tagaccgcac cagcggtgac tacaaccaat actggcagga ccgcaactac ctgctccagg
     7321 ctgacaaggt caagtgcgag gtcgtattta cccatggctc acaggactgg aatgtcaagc
     7381 caatccacgt ctggaatatg ttccatgccc tgccaagtca cgtcaaaaag cacctcttct
     7441 tccacaacgg tgcccacgtt tatatgaaca actggcaatc catcgacttc cgcgagtcca
     7501 tgaatgcctt actcagccag aaattgctag gctacgagaa taactatcaa ctaccaactg
     7561 tcatctggca ggacaacagc ggtgagcaaa cttggacgac cttggacacc tttggcggtg
     7621 aaaacgaggc tgtcttgcca ctaggtactg gaagccatac cattgccaat cagtatgccc
     7681 aagaagattt tgaccgctac ggcaaatcct accctgcctt ccaccaagac ctctatgcag
     7741 gcaagggcaa ccaagtcagc attgacctgc ctgtaacaga ggacctcttg cttaacgggc
     7801 aggtcacgct gaaactccgt gttgcttcta gtgtagctaa aggtctctta tctgctcagc
     7861 tattggacaa gggaaataaa aaacgcctag ccccaatccc tgcgcctaaa gcaagattga
     7921 gtctagacaa cggtcgttac catgcccaag aaaatctgct ggagttgcct tatgtggata
     7981 tgccacaacg cttggtaacc aagggcttta tgaacctgca aaaccgcacc gacctgatga
     8041 ctgtcgagga agtggtgcct ggacagtgga tgaatctgac ctggaaactg cagccgacca
     8101 tttatcagct gaaaaaaggc gatgtcttgg agttgattct ctatacaacc gactttgagt
     8161 gtactgttcg ggataattct caatggcaaa tccaccttga tttaagtcaa tcacagttaa
     8221 ttttaccgca ctaatactca atgaaaatca aaatcagact agtttcaaag gtttgggaaa
     8281 cctttgaagg ttggagatag agtgaacgaa gttcgtttct ccaagagcag atatacccct
     8341 gctactttct tattttatgg tagcaggctg tcaagctcca ctggagcttg acactcatca
     8401 aatcaagtca acaacgtctg attttgagtt tcgaagagta taatattgat tttcagttct
     8461 aaaaagaggc tgggcgtttc cgccctgcct ctttattgat atttcataat ggtttagatt
     8521 tgatctttag attttttgtt tatagctgta gcgactactg ccaagctaat tccagctaaa
     8581 ccaaggccta ctaaggctag gttattgcgt tgttcacctg ttgcaggtag ggttgctttg
     8641 tttgcagaag gtgcgccctt atgactcagt gtcagatttg attgtactga aggagttgat
     8701 tttacttctt ctttcttcat ttccaagcta ccttttacaa gaatacgatt ttgaccttca
     8761 acaacagtct ctgacaatag gttggttgca acaattttac catcaactat gacatcttga
     8821 taaacagata cttttttacc tgcttttcct tcttgaagaa ggtatgattt gccaagcgct
     8881 aggcttgcat catatctgat ttcttctttg atagggagag cttcttctac cttgcgttct
     8941 tttgttgtga agtctagctt tggtttttcc gctgtttttg gtgcgacttc tggaactgct
     9001 gtcttaggtg cagagtcttc cgcttttggt tgctcagcct ttggcatgtc ttctgctttc
     9061 ggttgctcca tttctggttg cttagcctct ggcatctgtt tgtcagactc tactttatca
     9121 gttttcggtt tgtccattgg agtttctggc attggagttg aggctggaac ttcttcctct
     9181 gctttcggcg ctggagcatc tggcgttttt tccactaaag tgccttttgc aatcagacgg
     9241 ttgactgggg ctttacggtc ttctgaaaga agattacttg ctatgacatt cccttcaaca
     9301 atcacatctt ggtatttctt aaccaattct cctttctgac cttcacgaag tacctttgtt
     9361 tgaccgagtg gcaaactagc atcatcttgt tcttcaacca tataatctaa aatttctcgc
     9421 tctactcgtt ctttaacggt aaagtctaat ttaggttttt caggagctgt cggtgctgta
     9481 tcaggatgtt tctctactaa aatgtttttt gcaatcagac ggttaactgg ggtcttacgg
     9541 tcttctgaaa gaagtttact ttcaatgact ttaccttcaa caatcacatc ttggtatttc
     9601 ttaaccaatt cacctttttg accttcgcga agaacctttg tctgaccaag tggcaaacta
     9661 gcatcatctt gttcttcaac catataatcc aaaatttctc gctctactcg ttctttaaca
     9721 gtaaagtcca attttggttt ttcaggagct gtcggtgctg tatcgggatg tttctctacc
     9781 aaagtacctt tagcaatcag atgatcaact ggatctttac gttttttgga aatgagggta
     9841 cttgcaacaa ttttatcccc aacaatcaca tcttggtaag tctcagactc ttcgcctctc
     9901 tcaccttttt gtcgttccac tttctcacca agtggcaagt ttggatcttc ttgctctttc
     9961 gttttgaaat ctaaaaattg atatgttgtt cgtgtttgaa ctttaaaatc tagttttggt
    10021 ttttcggcta ctttaggcgc tgtatcagga actttctttt ggaggttctt gctctctagc
    10081 tcgttgactc tgtttacaat tctatcaaat gcttgttctg cactatcaac atacgcttca
    10141 aaaacttttc cttcctgact agcataacta ccattttctt ctgattgttc taagtgctct
    10201 ctgtattcac ttctaataaa ctcattacca gagtactttt caagatatgg aacaacttga
    10261 cgtgtcaaac gctccatcct gatatggttt ttaaagtcga gtaatttatc ataattttct
    10321 ttgattttag gactatcttt ctcaagggtt tggacataac tcattagttt ttgcccgtct
    10381 tctaaaaggt atgtccatag tcctttttga aatgaataat cagagccagt gtagtctcta
    10441 aataccttta attcttccaa atctcgttct aactctttta gaatatcctg aaattgtgga
    10501 aggggagcag acttaacttc ggtattctct gatacttctt ccttagcttc gcttgtgagg
    10561 gctgctggag caggagttgg ttcttctgat ttttcaggtt cagagactac attttgttct
    10621 gcagagttcg ttgctgtttc ggctttttca gcaactggcg cttcttcctt aacttctggc
    10681 gcaacttttg attcagaatt tgtattctct gtacttactg ttccagtaat ctggactgtt
    10741 ggctctgttt cactcgttgg ctgagctgct tcaacttgag tagctgttga agtctctgtc
    10801 gtcgcaaaag cggttggtgt cgagactccc gcaccaagta agcaagtagc aatcagcacc
    10861 gagcctacgc cgactttaaa tttacgaata ctatatctga attttttatt tgcagtttcc
    10921 atttctatca ttcctaatta tattttatta tatagaatca ttataccacg atattcgagt
    10981 gactagatgt gttttttatg tgtttaaatt ctactgaaag actctgtttc ttcatcgttg
    11041 aatagatgtg actatttttc caatggatgt ataatcacac cctgcaccac acggttgacg
    11101 gtgcctgttg gtgatggtgt atctggcaag ttgttgcact taatggctgc catgatggat
    11161 accgtttggg caaagaggat gtaagggaag gttaggaaga catctggcag atctgctccg
    11221 ccttctgcca agacaaagtt ggctgcttct gtaccgacta acttatcagc cgacaatgcc
    11281 actatccgct ctgctatgcc atcaccatga acttcattaa gcaagtccac atcatactga
    11341 cgagtgtagg catcgtttga ggcaaagagg aaaatcaagg tcttttcatt gatgaaggat
    11401 tttggtccat gacggaaacc gagtggtgac tcgtacatgg tcgcaatctt acctgctgtc
    11461 aattccagaa ttttcaactg agcttcgtgg gcaagaccat agaaaccacc tgaaccgagg
    11521 taaatcactc gctcaaaatc aagattaatc aagtcttgca cgtagtcttc acggtccaaa
    11581 acatcttgac ccagacgggc aatgatctca acccaagcag ctttttcttc ttgactggct
    11641 ggcgagaaga cgaggagggc tgtcagagcc atacagctgt aagaacctgt catggcaaag
    11701 cccttatcgt ttgaaccagc tggttgcaag agtaggagat tttcaacatc tccttcagcc
    11761 gctagtgcca gcttgccttc tggtgcacag gtaatggtca cttgatacaa tttatctacc
    11821 aattgcttgg cgacttcaac tgctgccaca ctttctggtg aattgcctga acgggcaaag
    11881 gacaccagga tggttggaag atcagcttgt agatagtcat gtggacgact aaccagctcc
    11941 actgtaccaa tggcagaaaa acgaatgtgt ttcaagtcat tgacttggtt gaggtagttg
    12001 gcaatacttt gcccaacaaa gtcagaagtc cctgcacctg taaaaacaac gtgaacaaag
    12061 tcatgttttt ccttgattga ttttagaaaa ctctcgattt tttccaattg gtcaaaatag
    12121 agttgatgcg cttccttcca taagcctggc tgttggtaaa tttcggtcgc cgtaatcata
    12181 gctcccagct tttcaagttc ttcttttgct aaacggaaca tagctcttcc tccgtgattt
    12241 ctggttataa ccagtttttt cttatattat agcgaattta cccctcaaag tcaatttaaa
    12301 atagattaat atgtcaaatt tatatccgat attgccgact agaacttatt ttgagaaggt
    12361 agcattatct acataaaaca tgttataata tttactacac ctctgccttt gaaaattttt
    12421 aacaataagg caaaataagg caaggaaaaa ttacaaacaa ggtctaaacc cttgatacga
    12481 aaggaatctt aaaaatctta tgatgaatat gcaaaacatg atgcgccaag ctcaaaaact
    12541 tcaaaaacaa atggagaaga gccaagctga gttggctgct gctcaattta ccggtagctc
    12601 tgtacaggac ttggttactg ctacttttac tggcgacaag aaattggttt ctattgactt
    12661 caaggctgac gttgtagatg cggatgatat tgaaactctg caggaaatga ccgtccaagc
    12721 cgtcaacgct gcacttacaa aagtcgacga ggcgactcag aaaaaactag gtgcctttgc
    12781 aggtaaattg cctttttaaa aagaaaacgc gattggatac tctcctctcg cgattttttt
    12841 attcaaaatt ataaatcaaa gactgaaatt gctggaaaaa gaggtctgga tagcgtcgat
    12901 tctctttcat ttcctccatc agactttgga ggtcggtcat gttgtcagtg atgataccgt
    12961 ccacttgtag cagcaacatc ttggtcatac tctcttcttc attcggtgtc caagcataaa
    13021 ccgacttccc tcgaatccaa gacttggtca taaaattttg atccagcgaa gtgtattcca
    13081 ttgtataacc atcagcaacc gtcgtcggat aaattgaatt gaacggaaga ataaagaagg
    13141 atactaattt ctcgctatac tgctttattt cgataatagt acggtaatct aatgattgca
    13201 tctggtgtcc tttggcaatc aaacgttgac cgtatttttt taagaaattc tgggtgagtt
    13261 ttggcgaatc tgccttggtc actttaattt ctaccaataa tttctgtccc agctcatccg
    13321 ctttttcaag atattcatca aaagaaggaa ctggagctac catgccgttt tctgatgcag
    13381 tcatccctgt caactctgca agagtatagt catgtgtacc tccgggattg cccgtcaaag
    13441 ccaataaatc cgtatcatgc atgacaataa actgaccatc cttcgtctcc tgaacatcca
    13501 tctcaatata atctggcttg agctgagagg ttttctctag ggcctcgata gagttctgga
    13561 cagcattgcc gtcatcgaca cctcggtgag aaatggtaac cggcagagtg tcaaagggaa
    13621 agtagagggc aagcgctcct tgggtaccga acaccagacc agatacgagc aaaatggcta
    13681 gtcgaaggtg atgacggagg cgtttacggc ggcagattgg tagttgtttt cctgttagga
    13741 ggctgacaaa tcccatcatg aagagtgcaa ctactccgta gtaggctaat tttagaagtg
    13801 cgtaacagac aactgccagc cagtaagaag cacttggtac aaaatgctct gctaacagtt
    13861 gcacaagata gaatagtcct cctactcctg taaataggag aactggaaaa gtaatcaacc
    13921 aaattagacg gaacaatgaa ccgatctgct ttctgcctct ggtcttctcc caagaataag
    13981 cgatagactc cttcacaccc ctgtgttcaa aataaatgtt tggcagagcg tacatgaagc
    14041 gtgccgcaag ccagaagaac aagaggagga aaatcaaaat caatattcct agccagactg
    14101 tgttggatag gtaatctacg ataaattggg gcactacgat tttattaaaa taatagatat
    14161 ttaatactcg gcgtaaaaat gggagaagaa taactgagta aaagagcaga aatagaacct
    14221 ttggtaggct gctgtgacgg ataatcgcca ccatactatc tcgtacatcc cgtaaatagt
    14281 cggggaggtg cttgactttt tcatccaaca gttgccaaac ccctgtgaac aatagagcta
    14341 attctagata ggcaattgca atgttgacaa aaaagaggag aataaacaag cctgtcacga
    14401 gtagattttc agcaaacacc gacaacaaat tcgtcggtga aaggtaggca tgccctgtca
    14461 tagctaagag tccctcagaa atccatgaat tgagcggtat ccaaacaaat tccataaagg
    14521 taaacagggt aaagaagagc aagagaatct tgtctaggtt gtaatatatt ttttgaaatt
    14581 cacgttgtat ctttttcatg attctagtgt cccatattct tgcaagaatt gcaagccttc
    14641 atcaaggatg gaagattgtg aaagcaatga atgaagtata gaaaaaatca agttcctaag
    14701 tggagcttga tttcttacat ttgatttgct ttcctattcc caagccaaag taaatcgaaa
    14761 ggctacataa aatacgaatc actatctcac tattgttgta acattaattg tgcttgttta
    14821 acaatatatt ctgctgtcaa accatataca tccattaagt aagcttgttg cccaacttga
    14881 ccaaaagatt cctcaatacc aattcgatgt actggggtcg ttaaatctgt agccattaat
    14941 tcacaaatgc tactacctag gccaccaatt cgattatgat tttctacagt taatactggc
    15001 cttcctgata atagagctgg tatgtcttca tgaattggtt tgatacgaaa aagatcaatg
    15061 acttgaacag atactccttg agcctctaac tgatccgccg cctttaatgc ctgctctacc
    15121 ataataccac tcgcaagcaa ggtcatatcc tccccttgcc taaggacaca gtagcctttg
    15181 ctaaaatctt ctccaccttg ataaatagcc gttgtcgctt tccgaatggt acgaatgtac
    15241 ttcaacccgt ctaccttgat ggtttcttta agaacagctt gaaactggac atcgtcactg
    15301 acctcataaa cacgcgcttt tggaatgagc cgcattaagc ccagctcctc aaacggcatg
    15361 tgagtcccac cattcatctc cgctgatacc cccgcatcgg aaccaatgac cgtggcatta
    15421 agttgggcat aagctagaga gataaacacc tgatcataga ctctacgact agcaaatggt
    15481 ccgaaggtat gaatgtatgg cttgtatcct agaagagata atccagctgc tacccctacc
    15541 atttcctgct ccatgatacc tacattgaca tagcgtccac ccagaacaga ggacaatttg
    15601 ttggtagcca ttgaactgga taaatctgct tcaatagctg ctatggttgt gtcttcctgc
    15661 cctacctgag ctagaaaatc actataaacc agtcgcatct cctttgtctg tctcattcca
    15721 tcacctccaa ctctctcgct aaaacaagag ttacttgctc taaatcttcc cgtaattgac
    15781 ccgacaaacg caaatggtga ttgctttcta attcttctaa gaaggtaact ccctgcccct
    15841 tagtagtatc aagaacgata cattttggtt gaggagactt acttgacttc attgttagaa
    15901 tagcatttat aatagcatct atatcggcac cattcacacg cactgattcc caaccaaaag
    15961 cctgaaattt ttcaacaaaa tcaaaggttt ggcaaatatc atgggtacga ccgtctagtt
    16021 gttttttatt gtcatccaca aacagaatca ttttcgatag ttcgtggtga gctgcaaact
    16081 gagctgcttc ccagcattga ccttcgttaa gctccccatc ccctaccaga gtataggtat
    16141 agtacggtga ctgttctatc ttcttaccat aagcaacacc tgtcgcgaca ctcatccctt
    16201 gccccaacga acccgttgtc atatccaccc ctggcgttaa gtttcggtca gggtgtgaag
    16261 gcaaatgtgt tccattttga tttagcgaca agagaaaatc tctatcaaag aagcctttca
    16321 aatacaacgt actataaagt gctggacctg catgtccctt agacagaata aagtgatccc
    16381 gctccttaga agagaaattc tggacacgaa tgtccagaat ttctccatat agagcagcca
    16441 acacttctac gattgataag ctaccaccat aatgaccgaa tcctaattga ttcaaagtcg
    16501 ccaaggtatg gtagcgaatt tctttggcaa atcgtttcaa atcagctgta ccactagtca
    16561 tattatcctt ctttctgttc ttttttcacc aatgacaatc caagcatcaa agccaagaca
    16621 gcaaagatac caaccgaaac tgtgaggata ccgccattct tagctagcac tcccaggaaa
    16681 ataccagaaa gaccaaagtc tgcatctgag aaagttgaac cagcaaaacc aaggtctccc
    16741 aaaacaggaa gtaagaatac aggtaagaaa ctaatgagaa ccccttgcat aaaagcacca
    16801 caaactgcgc cacgaacacc acctgacgca tttcccatga cccctgcagt tgctccacag
    16861 aagaagtgtg gtacgacacc tggaaggata acgacaccgc cagtcaaggc aagaataatc
    16921 atgctgacaa taccacctgc aaaactagag ataaagccaa tcaacacggc gtttggtgca
    16981 taaggataaa caactggaca gtctagggct ggtttggaat tcggaaccaa cttctctgaa
    17041 attcctttga aggctggaac aatttccgct aaaattaagc gtacaccaga taagataaca
    17101 aaaacacctg ctgcaaaagt tccacctagg gtcaaggcgt aaatcatgct gttagtacca
    17161 ccactaaggt tttccttaat ataatcaccg cctgcaaaca aagccacaat catgtaaatc
    17221 aaggacatag aaatggcaat gctgacagtt gaatcacgca agaatgccaa acctttaggg
    17281 aaattaatat cctcagtaga cttagactta tcaccgacca aaccgcctac tacaccactc
    17341 aaccagtaac caagtgcact gaagtgacct agggcgacat gatcatttcc tgtcaattga
    17401 atcatgtatt tttgaacgaa ggctggggag agtgtcataa tcagacctaa agccaaccca
    17461 cccatcacaa ttaggccgac agaagtgaat cctgcaacac tcatgataac tgcaatcata
    17521 caggccatat atagtgtgtg gtgtcctgtc aagaaaatat atttaaagcg tgtaaagcga
    17581 gcaatagcaa tattgaagac cattccaagc aacataatca aggatgtagc agttccatac
    17641 ttatccaaag ccatcgctac aatagcttca ttatttggaa caaccccttt caagttgaaa
    17701 gcttgttcaa acatttgacc aaatggctca agcgagccaa caataatatt cgctccgcca
    17761 ccaacaacaa taaaaccaac aaaagttttc aagcccccct taattaaatc tgctgcgggc
    17821 tttttctgta aggctaaacc tagaatagca atgagagcaa ccagaattgc cggagtttta
    17881 gcaatatcaa tgagaaaatc caataccatt ttctgtctcc tttataattt tatctttgcg
    17941 ggtcatcccc tgtgtatacg atttaaacca aacctttttc ttgacagata gccgtgacaa
    18001 gttccctcaa ctcattcata tcaataatac tattcaatat tcgaacatct cctaaatgag
    18061 ttgcagaatc agccaggtca cgtccgacaa tccacacatc tgcttcactc ggattggcac
    18121 ctcctagatc ataatgctcc acatgaacat ctgatacacc taaatctcgc aaaatggatt
    18181 caatattcat ttgaaccata aagctagagc ctaaacctga accacaggct gttccaattc
    18241 gtaacatatt actgttcctc ctttaataaa tgttgtacgt cggcaaattc tttggcgccg
    18301 acaagttgtt gaatattttc ttctgctcgc aaaatttttg tcaaatgcga gagtgctctg
    18361 agatgggttt gattatccac ggctgcaata caaatgaaca gccgaatttc atgactaggg
    18421 tcatccaata gataaactgg cttatccaat accaacattg acatccctac ctcattaacc
    18481 ccatcttctg gtcgtgcatg tggaatcgca attcctttac ctaggtcaat aaaaggacca
    18541 aactcttcta ccttcgctat catagcatca atatatcgag tttctatagc tcctttatct
    18601 accagaggtc gtgcaaccag ggcaactgct ttttgccagt ctaaaacttc ctcagaccat
    18661 tgaaaggtct cttttgtaat caattcttct aacataggac tccttcccct ttgctcatgg
    18721 gagcgttgat tgagaaactg caccaattca tattttaaag cctgctcctg atgaatggtt
    18781 gcatgtttcc caacaagtga taataactgc tcaatctcaa tcggaaaata agccgcattt
    18841 gggaaatgct gattaaccaa gtgaaataat tcttttttat gattagaggt catcagctgg
    18901 gataccaaga aaaatggtag ttctatattc agcttgatag ttccaaatac catgtcatag
    18961 tctgtcatgt ctagcatctt ccaatcctgt aaggaatggg tatcggcaaa atagatatta
    19021 ggaaatagtt gccgtagatt ttcttttaca atcagcgaag agctcactcc gtttgggcaa
    19081 acaaccaagg cccgataacg atatgaacgt tgttgacctg cttcgatgta gccaccaaag
    19141 tggatgacaa aataagatac ctcactatct ggtatagtaa aacctagatg ctcttccaaa
    19201 ggtcgcaaag ctttttttac aagatagaac aagtcggcat gctcttcttt gatggtttca
    19261 gtatatgaat tgacatttac caaacgagat gttaaacggt agtaagcagg aattaggtga
    19321 cgttgcaaac cttcaatcat ttccgagcga tgttcaaaag caattaagga caaacgttcc
    19381 atctcttcca ctatttctac agtcaagtgg taaaatagat catccttttc tccgctctca
    19441 ccttctaagc atccttgcag taaatgtgac agatactctt gtgtcgcagc atttaattct
    19501 aatgtgctag tactttttaa acgtaataat ttccaaaggc gttgcagaaa atcttttaca
    19561 aaccctccct ttccttcaaa attccagtta atacggttgc caacacgcgc ctgacggata
    19621 aataaaaata tcaaaaaata caaatattca tccaatctct cttctaaagc tgagacttta
    19681 taataattgc aagcttgttg actcactatt ttcaattctt caataggatt ttcctcaccc
    19741 caagatttaa gaatataatc caaagcccac acaccaatcg aagactgcaa acaattactg
    19801 agtgcataaa gcgccaagcg atgtttatct tcttctcgac caattagatg atagcctttc
    19861 gccctcgtat attccaatcg tacttgaaag tccaagcaaa gttcctttac attcttaata
    19921 tccgtcaatg ttgtgttacg gctcacactc aataattctt ggtagtgaac gtttgataca
    19981 aattcttttc ggataaaggt ataaagatag ataagaagct gtctttcttc ttgcgaaaga
    20041 tagatctggc gattgcgaaa gagcgaaaat acactttctt tttgattgta aagctccgta
    20101 gaaataacaa actcgctatc tttgtattgt atctgcggga aggaatgttc ttgcaacaat
    20161 tgattgacta gttccaaatt agaatgtagc tgctcctttt caattcctgt ttgaatacta
    20221 agttcgtaca aactttcttt ccgagtcgca aacaattcca tcagaatcgc catggtacga
    20281 ttatcaacca tattttcctc cttgtcttaa ttgtatccct tttcatttct gatttctaga
    20341 gtctttgagc ccaattaata taataaaaaa tacaaattca attgtgctca ctacttaaac
    20401 taaaaaagat tggttcccac caatcttttt atatcacatg ccatcttcca ttttttagtg
    20461 cgggctgaaa cagtctatcc ctagactgta cctcgctttc ttatttccaa gctcgggctg
    20521 aaacagtcta tccccagact gtttcactcc cccggacctt tattcgctct cttgctttca
    20581 agctcatgat aaaaaggtcc cccggacctt tttatttccc aaacaaacgt gcaaagaagc
    20641 ctttagcttg cacttcttct gcctgtgctt taacttcgtc caattccaac ttgagaactt
    20701 ccttatcggc catagctttt agtgtcaatt gctgttgctg atccaactgt ttatccttct
    20761 cagcaatctg cacatcttta accttgagtt gttcatcttt ttcggcaagt tgcttatctt
    20821 tagcttttaa ttgactatat aagcggatga tttcagcatt tttctcgtca actaaaattt
    20881 ccatcaattc acgatgcttg acttcatcac taatcggctc atcttcaaaa atggttgttt
    20941 tgtagatttc ctccaactta atcaagccag cacgatttac aacggtcaca cccttgtcat
    21001 ttttatctac aaattcttca ggaagtgatt tgacacgatt attcatagcc tgccggctca
    21061 ctcctaaaat ttcagctaac tcactgactg ttttttcgat tcccataata tcctcggtac
    21121 ttacatttct catagtttgt atcattaaaa tattaccaca gaaccctatc cctgtcaaat
    21181 ctaggggaat tttcttacca agcattagtg aataatcaca aacctcaaaa gcatcttatc
    21241 tcaatgcaat gttatgcaga aacgaaatat acattctatc taataaaacc atttttagca
    21301 ctcacttgct tgaaccaata tccagatttt ttgacagtac gcttctgagt ttgaaggtca
    21361 agcgagacca aaccgtatcg gttcttatag gtatttaacc aagaccagca gtcaataaaa
    21421 gtccagacca tataaccctt acagttcgct ccttcatcaa tacctcgatg gagctgaatc
    21481 aaatgatctt ccatgaagtg aatctgatag tcatcctgaa tcatgccatt ttcttcaaaa
    21541 acatcctccc cttcaactcc catgccgttc tcagttacca accattcgat attcccatag
    21601 ttttcctgga tatttttagc aatgtcgtac agcccttctt cataaatttc ccagcctcta
    21661 tggggattga ttttcttgtc aggcttatca taaggctgga aatattggga taggaaagta
    21721 gcttctccgt tccatccatc ttctgaagga gcctgcacgc gcaggggctg gtaataattc
    21781 acccccagga agtcaaccct attttctttg ataatggtca attcctcctc tgtggtttct
    21841 ggacgtaaac cgtgtttttc aagaatgtca accagttccc tcgggtattg tcccaaaaca
    21901 gatggatcca aaaagcttct cgtttgaaat agctcagcaa tccgcgctgc ctgtaaatcc
    21961 tctggactct gacttcttgg ataggcagga gtaagattga gaacaatgga aattttgtgg
    22021 tatgggtgta gttggtgaca ggcccgaaca gccaaagaac tagccagttg agtatgatac
    22081 gctacgacca cagctgcctt tgcatccacc ttgcaaggat aatgatagct tcctagatag
    22141 ccacattcta ctggaacaat gggttcatta aaggtaatcc aatgatccac cagatctcca
    22201 aagagactga aacactggcg agcataagcc tcataggccc agacaatctc cttattttcc
    22261 cagccccctt tttcctgtaa gacataaggc aaatcgaaat gataaagatt gaccaaaagc
    22321 tttatcccac gctctcgaat acctgcaaat acagccctat aaaactccac agcttgcgga
    22381 ttgacctcgc ctatcccttc cggaatcaga cgtgaccact gtatagaggt gcgaaaaata
    22441 ctatgacctg tttccaccaa caaatccaaa tctgactgga aatgttcata aaaagtcgat
    22501 gtttgttcca gtcctatttg ctggtgaaag agctcaggtt cctgactgta ccagtaatcc
    22561 cagttattcc acccctttcc atcaccagct acgcttcctt cactttgagg acctgaagtg
    22621 gaggagcccc acaaaaaacc ttctggaaaa cgatactctc tttgctcagt taccattctt
    22681 ctacctactt tctttcatgc tacagacgga gttgttagaa cctggattca caatgcagct
    22741 tccacaaaat tactttgctt gtacatacag gctcctgata aaaactaatt tcccttagtc
    22801 taagtatact cccctaaccc gtcaatggtc ttccgcaatc tcaaggaaaa actccacaaa
    22861 tcatagcaaa gactcaaact actttcttga taaaaatatg atacaataaa ggcatgaata
    22921 cagtagatac cattattata ggagcgggcc ctgctggcat gatggccgcc atttcttcca
    22981 gcttttacgg caagaaaacc ctgcttcttg aaaaaaacaa gcgtctgggc aagaaactgt
    23041 ctggcacagg cggcggacgt tgcaatgtga ccaacaacgg cacactagaa gacctgctgg
    23101 ctggcattcc tggcaacggc cgctttcttt acagcgtctt ttcccagttt gacaaccatg
    23161 acatcatgaa tttctttcag gaaaatgggg tcaagctcaa ggtggaggac cacggccgtg
    23221 tctttccgac caccgaccgc tcccagacca ttatcaagtg cttggagatg aaaatgttgg
    23281 aaaacggcgt caatatccgc acagggactg aggtggtttc cgtccgtaag attgatgacc
    23341 tcttccatgt caagaccagc gaggaaacct ttacggcacc tcagctcatt gtcaccaccg
    23401 gtggcaagac ctatccctcc accggctcga ctggctttgg tcacgacatt gcccgccact
    23461 tcaagctgga agtcacggag attgaagccg cagaaagccc cgttctgacc gacttccccc
    23521 acaagaagct ccaaggcatt tccctggacg acgtcaccct gacctacggc aagcacgtca
    23581 tcacccacga cctgctcttt acccactttg gcctgtcagg gcccgctgct cttcgcttgt
    23641 ccagttttgt caaaggcggc gaaactgcat ttctcgatgc tctgccgacc cattctgacc
    23701 aagatctgtt tgagcattta gaagccaaca gggaaaaatc cgtcaagaat gccctacgag
    23761 aactcatgcc agaccgccta gccgactttt tcgcggagaa ctacgactgc aaggtcaaac
    23821 aggtctccca aaaagacctg actgatctgg tctcccttct caaagcccta ccgatcaaga
    23881 ttaccggcaa gatgtccttg gccaagtcct ttgtgaccaa gggaggcgtt gacctcaagg
    23941 aaatcaatcc aaaaacatta gagagcaaga aggtcccagg cctccacttt gccggtgaag
    24001 tcttggacat caacgcccat accggcggct ttaacatcac ctactgtctt gcaactggtt
    24061 gggtggctgg aagttcctac tcttcgtaaa tcaaactcag acgttgttga cttgatttga
    24121 tgagtgtaaa gctccagtgg agctttacag cctgctaccc gaaaataaaa ggtagcagcg
    24181 ttctatctac atctaatggc acgaactttg ttcgccttat ttccaacctc caaaggttcc
    24241 ccgaacctgt ggaggtagtc tgagttcgat tttctttgag tattgcactt ttataaatcg
    24301 ctcttcaagc tcaacctaaa ggagaaacca tgcctatcaa ttcattcggc caaaagattg
    24361 gtatgccctt accgcattat agtagcggtg agcgaccaag tctggagact atacaaggac
    24421 acacagtccg cttagaaaaa ctgagcctgc accatgcaga tgatctctat cagttttatg
    24481 gaccaagtgc caagaaaact gactggacct acttgcccct tgaacccttt gaggacagag
    24541 cgacatttca ggactatttc cagactcttt tgaactcgac cgacccctat tattttgcca
    24601 tccttgacca agcaaccaat caggcagtcg gaacctttgc actcatgcga atagatacca
    24661 accatcgtac cattgaagtt ggctgggtca tcttttctcc gcaactgcaa cggacccgac
    24721 aggcgacaga agctcactat cttctcatga cctatgtatt tgaagaactt ggctatcgac
    24781 gctacgaatg gaaatgcgac tcgctcaacc aagccagtcg agtcgctgcc aaccgtctgg
    24841 gctttaccta cgaaggcaac ttccgtcaag ccctagtcta taaggaacgc aatcgggata
    24901 ctgcctggtt ttctctatta gaccacgaat ggcctgaccg taagcgtgcc cttgaaaact
    24961 ggctaaagga tagtaacttc gatggccagg gtcagcaaaa acaaccccta tcctctttct
    25021 aaattcaagc aaaatatgct tgaatttttt tgttttttgt tgtatgataa aaccataaca
    25081 atataacaaa aggagattgg ctatgcaact gaaattatct tctgtttcca agaaattcga
    25141 ccataaaatc attttggaag atacttcctt taccttcgaa caaggcaaga tttacggttt
    25201 gctagggcgg aatggggctg gtaagacgac cttgttcaac tgcattgccc gtaatctgac
    25261 cttggatggt ggctcgattc agtttgtgaa ggatgaaatt gcctttgact acgataatac
    25321 ggacatcggt tttacccaga cctatccgca actacctgcc tttatgacgg cttacgagtt
    25381 tgtccgtttc tatatggata tccacaagga taaactcaaa tctcctcgta gtccagaaga
    25441 atggctaaac ttggtcggta tcggagaaga agaccggcac cgtctactca aggacttttc
    25501 ccacgggatg cagaacaagg tccagttgct tctgtctttg attgtccagc cacctgtcct
    25561 tctcttagat gagccgctga cttcctttga ccctgtggct gcccacgagt tcaaacagct
    25621 gattcgggag gctaaaaagg attctgttat catcttttcc acccatattc tacaactggc
    25681 tcaggatctc tgtgacgaga ttgttttact acatcaccaa gaactacagg ctgttccgag
    25741 tgacaagctc cacgaccctg attttgaaca ggaagtggtt accctcttga cagcagacta
    25801 ggagggactt atgaaaacca ttctacgtta tttttggtac caggaaaagt ataacctcta
    25861 tcggaccgtc aacggatttt tctactatct gcgaaaactg ccacttgtgg gcaagagcat
    25921 tccagagagt attttcaagt cttacagttt taagtctggt ctctttttga tcctgcacgt
    25981 cctctccatc cccagtcgct ttctggtcaa gggaatttgg ttagccctga atttctactt
    26041 ggcatccttt tggatcaatc tcctctctgg cggggatttg actttttgga atattctacc
    26101 agggacttgg cttttgggct tttctctctg gatggtcttt gttggctaca cctatcggtt
    26161 tagcaagggt tttgagccct ttatcgtcaa gtccgagcgg gaattcatgc aaaattttgg
    26221 actttcccag tctagttttc tccagagtca actatttgta gaacctatta tcaccagtct
    26281 gttctacctg cctgccctgc tgattttttc tggcctatca ggaaattggc tctatctgcc
    26341 tctgggatta ctgaccattc cgacaggaag ttttaccgga caggctctca atcgttggtt
    26401 ttttaatcga cggattctat cccgccgtaa ttcttggcaa tcctgggtta tcttgggtac
    26461 tggtctagca gcaattgcct gccttatcct tttccgcaat catctgtcgc ccatcttttt
    26521 actgcctgtg cttgtctgtc aggtcttgct catttggttt ggctatggtt atttgaagca
    26581 acaaaccaac catctagact atctctatta ttgcatggac cagtccttgc agatggataa
    26641 gaaaattttt gagatgacca aagggaacga gtacacccga caaggtttac agatgcaggc
    26701 gaaactgagt atggaaacag gaaaggacct gactcatctg tcaggcatga cctacttaaa
    26761 cgctctgcta tttgaccgct atcgttctgt ccttacgaaa aaactgcact ttcgcttggg
    26821 ttgtcttttg gctattgtcc tttttattga aggaattcgt tggttttcaa aagatgacat
    26881 tcaaataagc aataccaact atatcgaggg actgcctttt gctttcatgg tcatgtatgc
    26941 cgcctccatg ggtaaggctg tcgcacagat ggtctttgtt aattgcgata tttccatgct
    27001 ccactatcca ttttaccgcg aagcaaaaac cattattgca ggctttcatt accgttttct
    27061 gcaaactgta aaattaaatg ctgccttctc tggatctcta ctattggccc tcatcatctt
    27121 tactagagcg cagctaccta ttgaaaccta tctcctgact gctctgctcc tgctgagtct
    27181 aacagcccta ttttccttcc acgacctctt tatctactat attctccaac cctttaccaa
    27241 ggacatggag gttgtcaacc cagtctataa atttctcagt ggggctctct actgggtggc
    27301 ctatctcaat atcaagctgg atttaggcag tcacatgtac atcctactca tttccatagc
    27361 tatgattacc tatgtcagca tcggctactg gattttgttg aaaaaagctc cgcagacttt
    27421 ccgcttgaaa tcataaaaaa tagcctctcg caagagaagg ctgttttctc gtcatttagt
    27481 ttctctttta ttacttcgac gaatgaggaa actttgtttc cttatttcca acttcaaaca
    27541 ctccactgga gtgtttgaac tcgccttgcc tgattgaaac agtctggggg actgtttcaa
    27601 gtcaatgcct agaaataaga aagcattgag ttatgtctgc tgcttttgat gtgaacatcg
    27661 ttcactttat ccccagtctc caactatctc ccagatagtt ggagcgagta ctaaaagtaa
    27721 actaaaagac tatatttacc agatccccaa aattgcttgc caaaccagtg tcaacgctgt
    27781 taccccaaac cagcaaacta gtccgactag gagagccgct ctgccatttt tcaccaaggc
    27841 aaacacattt gtccgcaaac caatcgccac catagccata caaatgaaaa ctttagagag
    27901 ggacttcagc ggagcaaacc aatctgcccc tataccaaat ccacccgcta tcgttgtgat
    27961 tagactagct aagataaaat acaagataaa gaccggaaaa cctgccaaga gggacttctt
    28021 gtctgcttga actcccttcc ctcgggtttg ccaaattgct aagaccgttg taatcgggat
    28081 aatagctaaa gttcgtgtta acttaaccgt cactgctgta tctaaggtct gactacctaa
    28141 accatacaag ctatcccagg tcgaagccgc tgctgtcaca gaggaggtat cattgaccgc
    28201 cgttccagcg aacatcccaa agacctgacc cgaatcagtc gaaaacccaa gccaagttgc
    28261 caaagtagga aagaccaagg ctgccaagac attaaagaga aagatgaccg agattgcttg
    28321 tgccacatcc tcatcatctg cctgaataat gggagcagtc gccgcaatgg cagagcctcc
    28381 acagatagag gtacctacgc caatcaaggt tgccagatga gatgaaatgg gcaagatttt
    28441 ccacatcagg aatgccaaaa tcaaagcaaa actaatggtc gaaaaaataa ttggaagaga
    28501 ttgacgccca actgccaaga ccgctgataa gtttaatcca aatcccaaac aaatcacagc
    28561 atactgtaag attttcttag aagtaaaggt taagcctgac tgaaatgcat ttttacttga
    28621 aaaataggga tacaggctca tcccaattag aagggcaaaa acaggcccac ctacaagtgg
    28681 aaataaatga cctaaccact gacccaccaa agccaaaagg aaacaaagtc cgacaccttt
    28741 ggcattttct tttaccataa aaacctccta aaatatcaaa cgtaacaacg cggttgtaac
    28801 aacgccaacc aagactgcta aaaggatatt ttttgttttc aaaaccacaa aaaatgtcgg
    28861 aagaatagct aagctttcca cagggagaaa acttggcaag gagccaactt tctcatccat
    28921 aatactggag agggtcaatg caaaaatgat agtgattggc aaaaagctga ggaatttcac
    28981 caactttgga gggagagatt tattttgcgt caggacaaaa ggcaggacgc gtggcaccca
    29041 ggtcaccagg gccgccatta gaataatcag taaaatgtta gtttttatca tcaatcatca
    29101 cccccacaaa gcaacctaat agtgtcgcca agagcaccgc cacataggac gacaccatca
    29161 tagacagacc gatataggtc agaccgactg ccagcagaat taagccaatt tttttcagcg
    29221 gaatggtccg aaccatagcc tctaactgac ctgcaaaaat accgacaaac atggcaacca
    29281 aggcaaagtc caatccaaac tgctcaggat ttggaatgag accaccaatc agattgccga
    29341 gagtcgtgaa tgtaacccaa gaggcgtagc ttgcaaaatt atttccgtac atccattctg
    29401 gagtaatggt atgatgctca atctgcttac gcaaaagaac tgcataggat tcatccgtca
    29461 caaaggatcc tatcaagata ttcgacccta aacgatttcc ttgaaaaata gttgtcgcat
    29521 gcaagcacat cagaaaattt cgcaggttga caaagaaaac ggttactgca atggataaca
    29581 aaggcgcacc tgcaaaaatc atagcacaca taacaaattg agcactcccg gcatagatta
    29641 ataggctcat cagccccatc tccacagtag aaatgcccga attaaccgat acgactccgc
    29701 aggctagacc aatactggca tagcccaata cagtgggaat ggcatcgcgc atcccttcac
    29761 gaaaagcata tttcttcatt cattatctcc cttcctctca ctaattgatt gaagaattat
    29821 tctctcattt tctcacattg caacccgttt ttcaagaatt cttatatcta atactcaatg
    29881 aaaatcaaaa gtagcctagg aaacgaagcc gaagatagaa ctggagttca tcaaggcaag
    29941 ttgacaacgg ataattttga ttttcgaaga gtataagtag aggtcttgcc ctcattgatg
    30001 tactttctgc ataaaataga cccagccgat agctgggcct agggataggg atgctactgt
    30061 tgtttctgaa ggaccatcaa ataagaagta aagtccttat tgtctccaac atggtctcca
    30121 tgtcgaatcg acaaacctgt atgcattaat tggctaccag tataaacttc tccatcaagg
    30181 tgatagaccg cttcctcttc caatccctgc aaatacaagc gttgatagga gagattagcc
    30241 gttgttaacc gacggtaata acccacgatg acttgctgac catccaagga ttggacctgc
    30301 caagccgtta tttctccatc gaatggactt ttcagccgag taaaaatacc ctgttggaaa
    30361 agcttacggt gagccttgta aaaggcaatc tgctccttca tctcttccaa ctcttcttgg
    30421 ctacactcaa acaaatccaa ctcatagcca aaacttccaa agaatgcaac attggcacgt
    30481 gtgcttagtg gagtagcacg ccccaactgc tgattcggac tagctgacac atgacaaccc
    30541 gtgctattta gaggatatac catgctcgtt ccatattgga ttttcaagcg ttccatggcg
    30601 tccgtatcat cactgaccca ggtttgcggt gcatagtaga gcattcccgg gtcaaatcta
    30661 gctccaccac ttgaacaaga ttcaaacaga accgtcggaa aagccgctat caggctatca
    30721 tataaacggt agagattgag aatatagcga tggaaggtct ccccttgccg ctccgcctcc
    30781 cttgtgcagg taaatacctc tgtcatatag cggttcatgt cccacttgat ataatcaata
    30841 tcatgctcag ccaacagccg atggagttgg tcatgaatat tttgataaac atcctccctt
    30901 gacaaatcca acgtatattg gtggcgtccg tgagattgac tatgctgagg atgatggaaa
    30961 atccagtctg gatgcgctcg gtataaatcg ctgtctttat taaccatttc aggttcaatc
    31021 caaagtccaa atttcattcc catagcatga acttggtcaa taatccctgt caagccactt
    31081 ggtaatttct ctcggttgac cgtccaatct cccaagcctg ctcggtcatg gtttcgcttg
    31141 ccaaaccaac catcatccat gacaaagagt tcaacaccaa gacctgcagc ctctttggcc
    31201 aaggaaagaa ttgtttcctc atcaaaatca aaactcatgg cttcccagtt attcaagaga
    31261 acaggtcgct ctctatcacg ccaatagcca cgtaccagat gtttttggaa gaggcggtga
    31321 taggtttggc tcatgccatt caaaccatgc tcgctataaa ccataacaac ctcaggcgtc
    31381 tggaactctt ctcccttatc taatgtccaa gaaaaacgct caggatggat tcccatactc
    31441 actcttgttt tttggaaaga actcacatcc acctgcgcca aaaagtttcc gctatagacg
    31501 agcgagaagc ctagtacctc tccctgttgc tcatccgcag ttgggcgttt caaagccata
    31561 aagggattgt gctccgcact actagcccct ttgagactgt aaatagactg acaaccttga
    31621 tgaagaggcg atagttgcag atgccgctca cgcccccaag ccccatccaa atgcaaccaa
    31681 tcatagtcca tatcaggcag acaaagtgtc atactcaagg cacggttcaa acagacagat
    31741 tgttctccga gttgttcaaa acgagcagag cgagtcaccg ctggataatc tcgaaaaatc
    31801 gtataagaca agaccaggcg ggtctgcgac tcttcatcca acaaataaat atccaaacta
    31861 tctgcctgtc cctcctcatc cagatagaga tgaggaagac ccttcaaagc cgatcgccca
    31921 gcataaatca catgaccttg atacacaaag tgcgacaggc tactaccgtc cttctgcacc
    31981 aactcaaaag tcggagaaaa atagtccgtt gtaccataac aagcaaattc aggattggca
    32041 tattggagcg aaaagaaacg gtcatcgtct tctacaaagg aagtcaaagc acgatactca
    32101 tcttcacgca catactgata ggaagctgtt tctgttacac gctttccata atacaaacta
    32161 ccaatatgac cattaggcaa gaccgtcaac acatagctaa taaactcatt gtaaagatgg
    32221 aattcctttg tcttttcatg aaagataatt cccattgtag cctcctataa ttttccacca
    32281 gatgtcgcaa tcccttggat gaactgcttc tggaatacca catacagtac caacatcggc
    32341 caaatagcca aaacacttgc agccatgagc tgaggatagt taacagcgta agctccctga
    32401 atcttagaaa gtgcgctaga cagggtagcc ttgtccgctt ctgagttgat aatcaatggc
    32461 cacagcaaat ccttgaaagc aaacaaagcc gtaaaaatcg ctaaggcaat caagccagac
    32521 ttggccaaag gcaacatgac cttaaagaag gtctgcccga tattacaacc atccaatttt
    32581 gcagattcct ccaattcttt tggaagaccc atgaaaaatt gacggagcaa gaatgtccca
    32641 aaggcactga caattcctgg gaataccaga gcaaaaatcg tattgcgcat accaagttgg
    32701 tcaatcatca aatactgagg aatgacgaat aactgaggtg gcaccatcat ttgaaagagg
    32761 accagaccaa acaagaaatt ccgcccagga aactccagtc gagcaaaggc ataagctgcc
    32821 atggctgaga acatgaccga gctaaaaata cgccataaca tcatcaaaat ggtgttcaga
    32881 taaagtatcg ggaaattatt agtccgaata gcctccgtat aattgctcac catccagtta
    32941 cttggtaaaa agtggaaagg attcatctga gtggattcgg ttaccgtttt aaaagacgtc
    33001 aacaccatcc aaacaaatgg gatcagcatg cccattgcca ctaaaatcaa gaaggcatgc
    33061 atccagattg ttgatttaaa agctgattgt ttcacatgaa cctccttaac ggtaatgcac
    33121 ccattttttc tgtaccttca tctgaacgaa agtaatgaca agaataatta ataacaacaa
    33181 catgacgatg gtggagccat agcccttatc cgaatattta aaggaattat tgtaaaacag
    33241 ataaaccaga gatactgtct tatcataggc aggactacga acatcctcca tcatatagat
    33301 aacatcaaag acttgcatgg attgaatgac actggttacc acaacaaaga acagggttgg
    33361 tgaaaccaag ggcagggtaa tggagaaaaa ttgcttggcc ggactagctc catcaatgcg
    33421 agctgcctca taaaaatccg ttggaatttc ttgcaaacca gctaaaatta aaatcatact
    33481 ataaccaacc gtactccaaa ttccaatcaa agcgattgag tatagagcaa tctttggatc
    33541 ttcaatccag ttcacagcac ccagtcccaa acgacgcaag atatagttaa tcaagccgaa
    33601 gtccgtattg tagagccatt tccaaaccat ggtcactgcg gctggtgctg ccaccatagg
    33661 caagaagaaa atggttcgat aaatatgctt acctttaatc ttagaattaa gcaaaactgc
    33721 aagcaacatg gccaaggcaa cggtagctgg aacaactaga atcgtgtact tcaaggtatt
    33781 ccatgtcgcc tgccacactt gcgcatcccc taacattcgt tggtaatttg ccaagccgac
    33841 aaaaatatcg cctttaccga aatctccact cttatgaaaa ctcatcttca tggtttggaa
    33901 aataggaata atattgagca cgattaatcc aataatggtc ggtgctacca ttccccatcc
    33961 ccaaattgcc tcatttaaac ttcctttttt tcggaacatc ttatccccat ccttcttcat
    34021 tttaataaga aggtccgggg aagaatcaac ttcccaagac ctcctaccta tttttcagtt
    34081 gctaattcag cattcatcat atcagctgtt tctttggcag cttcttcagt tgctttttct
    34141 ccaagataag caggtttcaa tagctcataa gccttatctt cccacttgat ggttgtttgt
    34201 gaatatggac gaatctgtgc ataatccacc atgtcaacat aattcttgat agtaaagttt
    34261 ttgtttgaat ctacccaagt atctgccgcg cctttataag ccgagattgc cacaccaagt
    34321 gttgcctgca tttcttgtgc ctcttttgag ctgaggtatt caacccattt ccaagcagca
    34381 tctggatgct tagtggtagc agcaatggca tgacccaatc cattaaagat ggttgcacga
    34441 gttccatctg gaccttttgg caagacagct acatcagcat tttcacgaat gtagtcatta
    34501 gcagtgaaac cagataaatt ccaagaaccg aagacgctca tagccacttg accgttttgg
    34561 aatgcttcgg cccgctcttt atctactgta atagccggtg aaattttttc tttgacaaag
    34621 ctaaagtagt aatccaaagc ctcaatggtc ttaggatcag catagcctga tttcaaatca
    34681 tcagtaataa ccttaccacc attttgatag ataaagttgt agtagccctc ttggttactc
    34741 aaaccagcac cgaaaccata ttgactacca tctggcttgg tcaatttctt cgctacttct
    34801 ttcaacttat tccaatccca agtatcatct ggatatggaa tacctgcttc gtcaaataat
    34861 tttttattgt accaaagacc gattgtatcg aaatcttttg gaatagcgta ctgcttacca
    34921 tttatgttgt agatttcatt caaaccatct gggaaattag ctagtttagc atcttcactc
    34981 ttggctaagt attcatccaa tggcaatagc atttcattgg aaccatagcg ataaatctca
    35041 tttgaatgca tccagaaggt atcaggcaat gaaccacctg tcgcaccagc ttctaacatg
    35101 gtccagtagg aatcccagtc cgatacttga atattgattt taatatctgg attctttttc
    35161 tcaaactcat ccgcaatctt tctcaaacca ggctcttgac cagaatccca aatagcatag
    35221 gatatttctg tctttcctga actacttcct tccgtagttt tgctagactc agtcgaagta
    35281 gaacacgctg ccagcaggcc tgcagccgca agcaaggtaa catagcgacc aattttcttc
    35341 atgataatac tccttttctt ttgtcgaata tgtcgacgaa ctttgttatt tctactataa
    35401 ctataacatg taagcgcttt ataatcaaat aggaatattt gacatattcc atatgatttt
    35461 gggatatttt ttaatttttt caaataaaat gtcacattta catataaata tgctatacta
    35521 tttgtaagcc tttacacttt tggtatggag aatacgatga aaatacaacc tgaattttac
    35581 gtctttaata acagatccta tgtagatttt taccccattc aatttggcat ggaagcctgt
    35641 aagccacttc actcattcgg cccaactatg aaacaacact acctgtttca ctacatcatc
    35701 tctggttcag gtacttttta cgatacttcc gaccaacgtg aataccacct aaccgcagga
    35761 cagggcttcc ttatctcccc agacgccatc tgtagctacg aagcagacga aaaggacccc
    35821 tggacctata tctggcttga atttgacggt ctgaaaactg agcattttct cagacaggca
    35881 gggctctcga aagaacaacc tatcttttca caacgagaaa tgcccacctc tagccctgtc
    35941 taccatgaaa tgaaacaaat tctcgcgctc catcatcaaa aaagcgctct agttctcgga
    36001 cacctttacc tctttatctc ctgcctcatt gactgttcgc taaccaaaac aagtagcaaa
    36061 cacgatgaaa acaaggaatt ttatatacga gaagctatca acttcgtaga acgaaactat
    36121 gagaaagcta tatccgtcga tgatttggca caagtatgca acctcaatcg ccactacttc
    36181 agtcggctat tcaaggagca aatgaacatc tcacctcaac aatttatcat ccaataccga
    36241 ctcagcgaag cctgcgaact cttaaaaaac accactaaga cactacagga aattgctgaa
    36301 gaaatcggct actccaacca attcaatttt tcaactgctt ttaaaagaca ttaccaaatt
    36361 tctccaaatc gctggcgaaa aatccacaaa taaacctctt acgtatcctt gacgtaagag
    36421 gtttttattc tataaattct caaaagccag actaggcttg gcattgaggt cccagccagc
    36481 aaggttttcc ttattgtact cactgaccgc tgctaaggca atcatacctg cattgtcacc
    36541 gcagaggcga agcgggggaa tgataacctc cacatcggta atttcagcag ccaaccgctc
    36601 tcgcaagccc tgattagctg ccacaccgcc cgctaccacc aaggttttga cagggtattg
    36661 ctccaaggcc ttcttggtct tagccatgag aatgtccata acacaggctt ggaaagaggc
    36721 tgacaagtcc gcattggaca aggtctcccc tttctgctgg gcattgtggt agaggttgat
    36781 aaaggcggat ttcaaaccag agaaagaaaa ctccagatta tcctctttaa tcatagcccg
    36841 agggaagtca taaatgtctt gcccctcatg tgccaattcg tcaatcaccc gaccagctgg
    36901 atagggcagg cccatgaccc gccccacctt atcataggct tcgccgaccg catcatctcg
    36961 cgtttcaccg acaatcttat agtcgcccgc ctctgacaca tagaccaatt ctgtgtgtcc
    37021 accgctgacc aagagggcca agagcggaaa ctccaactcc ttgaccgcac gcgccgccat
    37081 caaatgccct gccatatgat tgacaggaat gagtggcaga ccgttagccc aagcaaatgc
    37141 cttggcagcc gaaatcccaa ctagcaaggc accgaccaag ccaggcccat aggtcacagc
    37201 cacagccgtc aggtcctctg ccgtcacctc tgcctccaac aaggcctcct cgatacaagc
    37261 cgtaatcact tccacatggt gacggctggc cacttcaggc accaccccgc caaaccgctg
    37321 gtggctggca atctggctag caatgacatt ggacaaaagc tccgcatcat ttcgcaaaac
    37381 agccaccgag gtctcgtcac aggaggtctc gatcgccaaa atcaatctat ctttcatcac
    37441 gttctctctt cattagcacc gcgtcctcga caggatggcg gtagtagtct tttcgttttc
    37501 ctattttggt aaaatgctgg cgtgtataga gggcctgagc tgcactattg gactccctca
    37561 cttcgaggaa gatgtccccc tcccagtcca tcacagcagc catcagttga ctggcgattc
    37621 ccaacctctg aaaatcagcc ttgacagcaa tctgcaagat ttccatctca tccagcaccg
    37681 tctgaacagc caaaaatcca actaattcct gaccctcata ggccagataa taatcctcat
    37741 cctgactggc taggctagtc acaatctgtt ccaaggtcca aggcgattgc tcatagaccg
    37801 actccatgac tgccaacaca gcttccgata gattttcctg accgtcgtag tacttaatct
    37861 ctatcataca cgcttgatat aactttctga gccagcttgg ttgtccttga gccagttttc
    37921 ctctgcctct acccgcttga gatagttggg ctcaaagctc gtcacatcta ctgctggcaa
    37981 gtcttgaccg attactgcca attgataggc agacggtaga ctggcttgat actgagcagt
    38041 cggcagagct tgctcgattt gctcgataaa gtttgctact tctccgacaa aggtgatgtc
    38101 ttccttccca gacaggctag aaagtaagtc ttcaaaagac cagtacttgt ctgcctcaac
    38161 tgctctgcca ttttcataaa gacctgcata gacgcagtta cggcgggcat caatgagagg
    38221 caccaccaga cccgtcaggc tgggaggaac cagagactga agactggata gccccaccaa
    38281 gtcaatcttg agggtgtagg ccaaggtctt ggcggtcgcc accgccacac gaagacccgt
    38341 gtaggaacca ggtccctcag ccaccacgat cctatccaag tctttgggcg tccatcccac
    38401 ctgtgctacc aaaaaatcga ccacaggcat gaggctgata ctatgatttt tcttaacggc
    38461 cagcagggtt tccgcctgca aacgaccgtc ctccaccaag gcaactgata aagcctgatt
    38521 ggagctatct aaagctagta ttttcataaa tttctctttc tgtacaatgc taccctctat
    38581 tgtaagctat cttgaccttt ttttcaaata atgaaaagaa tcggttgtga tttctagtag
    38641 gttaatggat cgaaccgatt tgtcattcta gtaagtcaca tcactatctg gcagttgaca
    38701 ggcatttctc agcacaattg aacttagtct tagtaatccc aacacactcg gaatcctaac
    38761 tcatttcttt gatttatccc cttctgaaat accgaatttc tgaaaatcat gacacttttc
    38821 cattttccta ccaaaccctt ttaccatcag catattttat gatataatga agagtacatt
    38881 tagactgaat aaattgacaa ctcgagaaaa ttgaatagat attaccagac aga
//
LOCUS       DBNFEG010000002        37106 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.002, whole genome shotgun sequence.
ACCESSION   DBNFEG010000002 DBNFEG010000000
VERSION     DBNFEG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 37106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37106
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.002"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            121..462
                     /locus_tag="AC1PNG_000033"
     CDS             121..462
                     /locus_tag="AC1PNG_000033"
                     /inference="COORDINATES: protein motif:HMM:NF019021.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HIP6700579.1"
                     /translation="MAVLHHDQIQIGDDWTVKLFEFDPEDYKGKVHGWQREAPNEVNE
                     ILKAINAIAKPRHQAILIMSYILPEKIRSAKQAQRLGIAASTYYLAKNEALKEFAGQY
                     RDGSLLQYLDS"
     gene            880..2133
                     /gene="yfmF"
                     /locus_tag="AC1PNG_000034"
     CDS             880..2133
                     /gene="yfmF"
                     /locus_tag="AC1PNG_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P 5-aminopentanol modification-associated
                     protein YfmF"
                     /protein_id="HIP6700580.1"
                     /translation="MKLQEGVDLHFIDTDQFTTNRIRIRFAAEMSEATVAGRVLVANI
                     FEMGNQEFQTAQAVRRRLAELYGAQFSTSVSKRGRVHCVDVTISYVSPRHLPENEDIT
                     VEILDFLYTCIFRPLKKGRGFDSQIFEVEKTNLINFLQSEIEDNFYHADVEMSKLFYK
                     DPSLQIPRVGRLDLVEKETAESTFQIYRNMLRMDKIDIFVLGKVDREQVKRKLEDFGF
                     TYRNPKLELEYNQEYSNITQEKIERKQARQSILELAYHLQVVYNDVNYPALMVFNGLL
                     GAFSHSKLFMNVREKESLAYTIGSQVSIFSGMLKVYAGISHENRLRVMKLISKQLLDL
                     KCGKFTEEELELTKNMLIHSATLAQDRQNNLIEQVYNQVTLGNRNLSWLDWIEAIKSV
                     SIEDVIRVGQMINLQAVYFMEGTEE"
     gene            2130..3413
                     /gene="yfmH"
                     /locus_tag="AC1PNG_000035"
     CDS             2130..3413
                     /gene="yfmH"
                     /locus_tag="AC1PNG_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P 5-aminopentanol modification-associated
                     protein YfmH"
                     /protein_id="HIP6700581.1"
                     /translation="MKLVEKKYPYMKESVFYGKVDNGLTVILLPKVDFHETYGILTTN
                     FGGIHTRFTLANGKSVVYPAGIAHFLEHKLFETENEEDIMNEFAKLGASANAYTSFRQ
                     TSYLFSTTQKVLESLSLLQSFVREPYFTEDNVEREQGIIEQEIEMYQDDADYRLFTGI
                     LSSLYPESPLAYDIAGTVESIAAITADDLHENFEVFYHPSNMNLFVIGNFDLEAVWKQ
                     ISSYQVAQMDNPAQSFELAGVQKLPIQEHLSEQFEVSTPKLAVGLRGNDEVDKETIQK
                     YRLSLQFLFAMLFGWTSKRYQQLYEQGKIDSSFQFQLEVTPEYHYLIISGDTQEPITL
                     SSILMKALRKFEDDADVTEDHLQLLKNEMYGDFIRSLNSLEFTASHFVSQYSEYENVF
                     DIPQMIQSLQLDDIREAGRRFIDHCDMTDFTIFPK"
     gene            3495..4352
                     /gene="rodZ"
                     /locus_tag="AC1PNG_000036"
     CDS             3495..4352
                     /gene="rodZ"
                     /locus_tag="AC1PNG_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HIP6700582.1"
                     /translation="MRQKSIGEVLRTAREGRSWTFVDLQRITKIQAKYLQALEYNDFD
                     FIANSDDVQSILKAYAEALELDADVLLDAYETNSLVKYYEDGEEELRSSELKRSYKVR
                     KRKKNSYLPLIYLLLATGLIIIFVTYIVHSRLQNRASITPTTTSYSIVEQSVSEASSG
                     TETSSTEQAQSNSTSSSSSVTENVTISGSGSNIVATLKTVTYPVEVTVTAKNTTSWIS
                     LSDTLLEGGVTLTPDNPTVTTTVEEGVTSTTLILGVVKGVEVTVGGQKLNLSALTADT
                     GSITINFEQ"
     gene            4362..4901
                     /gene="pgsA"
                     /locus_tag="AC1PNG_000037"
     CDS             4362..4901
                     /gene="pgsA"
                     /locus_tag="AC1PNG_000037"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIP6700583.1"
                     /translation="MKKENIPNALTIGRILVIPIFILLLTVWDSTISHVLAALIFALA
                     SITDYLDGYLARKWQVVTNFGKFADPMADKILVMTAFIMLVELGFAPAWVVAIIICRE
                     LAVTGLRLLLVENGGTVLAAAMPGKIKTFSQMFAVIFLLLHWNAVGQITLYIALFFTL
                     YSGYDYFKGASFLFKDTFK"
     gene            4898..5734
                     /locus_tag="AC1PNG_000038"
     CDS             4898..5734
                     /locus_tag="AC1PNG_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIP6700584.1"
                     /translation="MMNIIEVKNLKYKYNQEDERYTLNDVSFHVKQGEWLSIIGHNGS
                     GKSTTVRLIDGLLEAESGDIYIDGDALTIDNVWDKRRLIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGIPLEEMRSRVDEALELVGMTDFKTREPARLSGGQKQRVAIAGVVALRPK
                     IIILDEATSMLDPEGRLDLIKIVREIKERHGMTVISITHDLDEVALSDRVIVMKNGQV
                     ESISTPNELFMREDLVDLDLDRPFTTELASSLRQTGLDLPLRYFTEEELEETLWELIS
                     KK"
     gene            5710..6552
                     /locus_tag="AC1PNG_000039"
     CDS             5710..6552
                     /locus_tag="AC1PNG_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002267082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HIP6700585.1"
                     /translation="MGIDLQEVSYTYQAGTPFEGRALFGVDLEIVDGSYTALIGHTGS
                     GKSTILQLLNGLNVPTEGRVVIDDVVITATSENKDIKQVRKKVGLVFQFPESQVFDET
                     VLKDVAFGPQNFGVSQEEAETIAREKLALVGIDESLFERSPFELSGGQMRRVAIAGIL
                     AMEPSILVLDEPTAGLDPAGRQELMDIFKKLHESGMTIVLVTHLMDDVANYADFVYIM
                     EKGKLVRSGKPVDIFQEVDFLESIQLGVPKITKFAANLERRGFVLERLPITIEEFTGM
                     VTHG"
     gene            6545..7339
                     /locus_tag="AC1PNG_000040"
     CDS             6545..7339
                     /locus_tag="AC1PNG_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIP6700586.1"
                     /translation="MDKLILGRYIPGDSIIHRLDPRSKLLAMFGFLLMIFWANNLVTN
                     ALLIAFVLGMVVLSRIRLSFFINGLKPMIGIILFTTFFQVFFTPGATILWEFWIFKLS
                     VEGLQQAAIIFVRFVLIIFFSTLLTLTTTPLSLADGIESGLAPLKKFKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQRARGVDFNEGNLIQKVKSVIPILIPLFASSFKRADAL
                     ATAMEARGYQGGDGRTKYRILEWKLADTLAILVMLALAGLLFILKK"
     gene            7471..8025
                     /locus_tag="AC1PNG_000041"
     CDS             7471..8025
                     /locus_tag="AC1PNG_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009911037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIP6700587.1"
                     /translation="MNITLKKNLKTTIAGLVAGISLLTAGVVSAETYTVKSGDTLSEI
                     AETYNTTVEKLAEQNKITNLDFIHVGQVIELGDVTVKAVESSIEQTQSTSTSTATVTT
                     STYSSNLSAEDAAAKEWIAMKESSGSYDARNGIYIGRYQLTNTYLNGDYSPENQERVA
                     DAYVASRYGSWSAAKAFWLANGWY"
     gene            complement(8482..9354)
                     /gene="sdaAA"
                     /locus_tag="AC1PNG_000042"
     CDS             complement(8482..9354)
                     /gene="sdaAA"
                     /locus_tag="AC1PNG_000042"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HIP6700588.1"
                     /translation="MFYTIEELVQQADNFSANVAELMIATEIELTGRSREEILTIMSR
                     NLTVMKASIVDGLTESKSVSGLTGGDAAKLDAYMKSGKTLSDSAVLSAARNAMAVNEL
                     NAKMGLVCATPTAGSAGCLPAVLGVAIEKLNLTEEQQLDFLFTAGAFGLVIANNASIS
                     GAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAICFVIKNMLGLICDPVAGLVEVP
                     CVKRNAMGASYAMVAADMALAGIESKIPVDEVINAMYQVGAALPTAFRETAEGGLAAT
                     PTGRRLTQEIFGED"
     gene            complement(9367..10038)
                     /gene="sdaAB"
                     /locus_tag="AC1PNG_000043"
     CDS             complement(9367..10038)
                     /gene="sdaAB"
                     /locus_tag="AC1PNG_000043"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HIP6700589.1"
                     /translation="MTNLKFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFGDEPT
                     EVEFQLYNSFAKTYRGHGTDVALVAGILGMDTDDPRIPDSLDIARERGIKVYWRVNKD
                     SNTPHPNTTRIIIKNDKKSISATGVSIGGGNIQVTELNGFSVNLNMNTPTIIIVHQDV
                     PGMIAKVTDVLSKYDINIAQMNVTREKAGEKAIMIIEVDARQCENSIAEIEKIPHLHN
                     VTFFN"
     gene            10253..11377
                     /gene="mnmA"
                     /locus_tag="AC1PNG_000044"
     CDS             10253..11377
                     /gene="mnmA"
                     /locus_tag="AC1PNG_000044"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIP6700590.1"
                     /translation="MSIDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDD
                     TDENGFCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDV
                     MCNKEIKFKAFLDYAMNLGADYVATGHYAQVSRDEDGTVHMLRGADNGKDQTYFLSQL
                     SQEQLQKTMFPLGHLQKPQVREIAERAGLATAKKKDSTGICFIGEKNFKEFLGQYLPA
                     QPGRMMTVDGRDMGEHTGLMYYTIGQRGGLGIGGQIGGDNEPWFVVGKDLSKNILYVG
                     QGFYHESLMSTSLQASQVHFTRDMPEEFKLECTAKFRYRQPDSQVTVKVKGDKAEVIF
                     AEPQRAITPGQAVVFYDGQECLGGGMIDMAYKDGEACQYI"
     gene            complement(11420..11809)
                     /locus_tag="AC1PNG_000045"
     CDS             complement(11420..11809)
                     /locus_tag="AC1PNG_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIP6700591.1"
                     /translation="MSTNLEIFNAYNNALIAGDFAALFETMADDIIWHQPGNHSTSGA
                     KIGKEVLGAHLATFAEKTNGTFKVVTNWVSDNDTLVAANVTFLGTRTDGEELNMNGID
                     LFRMEDGKIKEVWLFSADQTAEDAFWG"
     gene            11935..12261
                     /locus_tag="AC1PNG_000046"
     CDS             11935..12261
                     /locus_tag="AC1PNG_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIP6700592.1"
                     /translation="MKKVSEYGICPFATTQKVLTGKWGLVIIYQLSTGTKRFNELQRL
                     IPGITQTMLTRHLRQLEEDRIISRTVYAEVPPRVEYSLTEMGEKFRSVLDAVEAWGLE
                     YIETLT"
     gene            12403..12864
                     /locus_tag="AC1PNG_000047"
     CDS             12403..12864
                     /locus_tag="AC1PNG_000047"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIP6700593.1"
                     /translation="MDFRTKVDNQIFGVRATALLIKDGKIFLTKDSKGRYYTIGGAVE
                     VNEVAADAVVREVKEELGIDSHVNQLAFVVENKFTQEGIHFHNIEFHFIVEPIGEIPE
                     EMIEDNLKQACEWIDVDKLVNLDVVPVFLAEKLPNWSGQVTHIMNMKEKTT"
     gene            12861..14777
                     /gene="mnmG"
                     /locus_tag="AC1PNG_000048"
     CDS             12861..14777
                     /gene="mnmG"
                     /locus_tag="AC1PNG_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIP6700594.1"
                     /translation="MTHTFAENYDVIVIGAGHAGVEAGLAASRMGCKTLLATINLDMV
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGRNIDKTYIQMKMLNMGKGPAVRALRAQAD
                     KAEYAAEMKRTVERQENLTLRQTMIDEILVEDGKVIGVRTATNQKFSAKAVVVTTGTA
                     LRGEIIIGDLKYSSGPNNSLASITLADNLKELGLEIGRFKTGTPPRVNARTINYEETE
                     IQPDDEKPNHFSFLSKDEDYLQDQIPCWLTYTNATSHEIINSNLHRAPMFSGIVKGIG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTDEIYVQGLSTSLPEDVQQDLIHSIKGL
                     ENAQMMRTGYAIEYDMVMPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIVAGI
                     NAALKVQGKPELILKRSDGYIGVMIDDLVTKGTVEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGRQVGLVDDERWQVFQIHKNQFDNEMKRLESIKLKPIKETNEKVVAMGFKPLTD
                     ALTAKEFMRRPDVTYADAVAFIGPAAEDLDAKTIELIETEVKYEGYIAKALDQVEKMK
                     RMEEKRIPADIDWDDIDSIATEARQKFKLISPETIGQASRISGVNPADISILMVYLEG
                     RSRSISKNKSKDSH"
     gene            14884..16845
                     /locus_tag="AC1PNG_000049"
     CDS             14884..16845
                     /locus_tag="AC1PNG_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIP6700595.1"
                     /translation="MKRFRFSTIHFVMIGVILFGLVALVHRFFPIGASTVFALLISLL
                     VLIALFIYQKHSYEFSELEQIEYLNNQANSGLMMLLDKMPVGVIKVRPDSNQVEWFNP
                     FAELIFAQENGEFDQKKLREIIDVGLDEERIYANFSGKRYAVNVDFDQGLFYFFDAST
                     EYSATNSLIGSRPVIGIISVDNYDELEDAVTDSQISQVNSFLAQFVSEFCHDYGIYYR
                     RGTMDRFYFFTDYAVLEKLIENKFSVIDKFREEAKKLDLGLTLSMGLAYGNENHHQIG
                     QVALQNLNMAEVRGGDQVVVKENDESKQPLFFGGGSASSVKRTRTRTRAMMTAVSDKL
                     KSVDAVFVVGHRNLDMDALGSAVGMQYFAQNIMNNAYTVYNPDEMAPDIARAIKKLSD
                     ENCSNLLTVEEAMKMVTFQSLLIMVDHSKIGLTLDKDFYDQFTQVVVVDHHRRDTDFP
                     NNAVLTYIESGASSASELVTELIQFQNDKHHKLNRIQSSLLMAGIMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSLEIQQIAATDFEEYRQINELILSGQRIEANVVIACADDTKEY
                     DNIIPSKAANTILDMAGIEAVFVVTRNTKGYVSISARSHSKINVQRIMEEMGGGGHFN
                     LAAAQVYDQTIAEVCEKLTDKIREEINGN"
     gene            16856..17308
                     /gene="rplI"
                     /locus_tag="AC1PNG_000050"
     CDS             16856..17308
                     /gene="rplI"
                     /locus_tag="AC1PNG_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIP6700596.1"
                     /translation="MKVIFLADVKGQGKKGEIKEVPTGYAQNFLIKKNLAKEATNQAI
                     SALRGQEKSKEKAHAEMIAEAEAIKAKLAEEATLVKFVEKVGPDGRTFGSITSKKIAE
                     ELDKQFGIKIDKRHIQLDHPIRAIGLIDVPVKIYQDVIGIINLSIKEA"
     gene            17340..18695
                     /gene="dnaB"
                     /locus_tag="AC1PNG_000051"
     CDS             17340..18695
                     /gene="dnaB"
                     /locus_tag="AC1PNG_000051"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIP6700597.1"
                     /translation="MAELDELRVQPQDILAEQSVLGAIFLDEGKLVFVREYIEAEDFF
                     KHAHKLIFKAMIALSDRNEAIDATTMRNYLVNHGDLENIGGLAYLAEIISSVPTAANA
                     EFYAKIVAEKSNLRKLIARLTDAVNQAYEGADGSDDIIANAEKALIDVSEGASRSGFK
                     RIDDVLNINFDNLETRAKQTSDITGIATGYPALDAMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLGLTVAIFSLEMGAESLVDRMLASEGVINSRAIRTGHLTQEEWNKY
                     MVAQANLARASIYIDDTPGIKITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGEQEDGGIPNNTVEVIIEKNRSGARGTVELMFQKEYNKFASISKRED
                     G"
     gene            18699..18950
                     /locus_tag="AC1PNG_000052"
     CDS             18699..18950
                     /locus_tag="AC1PNG_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HIP6700598.1"
                     /translation="MSDAFTDVAKMKQIKQDIKDHEGQIVELTLENGRKREKNKQGRI
                     VEVYQSLFIVEFEDPNNTFVESYTYSDILTEKILIHYLD"
     gene            19161..19772
                     /gene="rpsD"
                     /locus_tag="AC1PNG_000053"
     CDS             19161..19772
                     /gene="rpsD"
                     /locus_tag="AC1PNG_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIP6700599.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKIKQGTLGFNFMLLLECRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVEVGQVISVREKSIKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            19839..20288
                     /locus_tag="AC1PNG_000054"
     CDS             19839..20288
                     /locus_tag="AC1PNG_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HIP6700600.1"
                     /translation="MWRKYFLLVLLAILVVGGAFFLSQSAATPNESDYVKAVKSYQAI
                     SIDEVEQKVQDEEEFILYIGRETCPYCRDFVPKLTEAVERSRATIYYLDSESDPNGKI
                     QQFRQSQGLATVPSLTYYKSGKLSGILRKGSQATLSEIENFLALQGQ"
     gene            20421..21938
                     /locus_tag="AC1PNG_000055"
     CDS             20421..21938
                     /locus_tag="AC1PNG_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HIP6700601.1"
                     /translation="MKKKAWLGLLMSLLAVLFLVACGGKSEKTATSSSSTTSSSSEEA
                     VSGASEKVYTDPSELKDSYDVIVVGSGGAGMSAAISAKDAGASVALLEKMPVIGGNTA
                     KSSAGMNASQTKFQEAEGIADTNDKFYEETLKGGKGTNDPELLRYLVDNSASAIDWLD
                     GMGITLSNLTTTGGMSEKRTHRPADGSAVGGYLVNGLYHNLVERKVPIFVNADVTKLQ
                     DKDGAVTGVEVKIAGETKQISAKAVVLATGGFGADLEMVAKLNPALKGYVTTNQEGST
                     GDGIALAESEGAATVDMEQIQIHPSVEQETSYLITEAVRGEGAILVNSKGERFVNELE
                     TRDKVSAAINSLDPNYAYVIFDDALKDRVKAIAQYEEKGLVKTGATLADLAKEIDLPA
                     DTLQTTLDTWNKAVADKNDAAFGRTSGMDHALNTGSYHAIKVAPGVHHTMGGVKINTN
                     TEVLKTDGSAISGLYAAGEVTGGVHGQNRIGGNAVADIIVFGRQAGEQAAGFAKK"
     gene            22024..23388
                     /locus_tag="AC1PNG_000056"
     CDS             22024..23388
                     /locus_tag="AC1PNG_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIP6700602.1"
                     /translation="MKKKIVLLLGAIAVIVLTWSAIAGQRQQSYAEAELRDQDKIWII
                     TDLHYLSQDLFDDGEAFSYIEKTAAGKDLRYGKERMEALVEQVGREHPSLLLVSGDLT
                     LNGEKQSMVELAQYFTEIEEQGTEVLVIPGNHDIASGWARAFKGDQQIVTDQVTAQQF
                     AELFAHHGYQQASSRDKASLSYLAKPFSNAWFLMIDSNIYSDGYGKGAPPTNGRINKE
                     TLEWIEIQLQAAKETGVSLIPVVHHNVLQQHAMLSKGYTLDNAADLKTLFNQYGIQFG
                     FSGHTHSQNIVKEDLGQVNYTEVVNGAFSIYPAIIGQLSLDDTSIHYQKTQLDMASWA
                     DKHQPSDPNLQDHVTYLQTVFDNTSDIMVHNMLNDEGWYDGETADAISQFIMPANRAY
                     FSGEKLDQTWLDEKVFPSQAYQTLQAASPRSFLADYLDVIIKRSQNRDVEKLVLKTEK
                     DFVQ"
     gene            23552..24565
                     /locus_tag="AC1PNG_000057"
     CDS             23552..24565
                     /locus_tag="AC1PNG_000057"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIP6700603.1"
                     /translation="MTLLSIAIPSYNSEAYLHYCVHSLVMGGDKVEILIINDGSSDRT
                     QEIAEELANQFSNVRAIYQENKGHGGAVNTGIREAKGKYFKVVDSDDWVDTRAYLKIL
                     ESLQALEDENTPVDAFISNFVYEKEGQSRKKSMSYQSTLPENRIFGWEDVGAFSKGQY
                     MMMHSLIYRTDLLREVGLVLPEHTFYVDNIFVFTPLQAVKTMFYLPVDFYRYFIGRND
                     QSVNESVMIKRIDQQLKVNRILVDNLDLEAIDNPDLRSYLLNHVEITTIISCALLNRA
                     GTAEHMMKKQELWHYIRDNNPALFKIVRKGLLGQLTNLYGYPGRKIFNAVYKIAKRIY
                     GFN"
     gene            24635..25546
                     /locus_tag="AC1PNG_000058"
     CDS             24635..25546
                     /locus_tag="AC1PNG_000058"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIP6700604.1"
                     /translation="MQRLGQTGLEVSRIGLGCMRMASLTEQETAKVLDTVVGQGINFF
                     DHADIYGGGESEIRFAQGAKLAGLKREDLLLQSKCGIRKGYFDFSKDYILESVDGILK
                     RLDTEYLDVLALHRPDALMEAEEVAEAFYLLKKAGKVHHFGVSNQNIYQMELLQSYFD
                     QPLVVNQLQLSPAHTPLIDAGLHVNMKDDAATMRDGGLIDYCQLKKITIQAWSPFLID
                     LQRGIFANHPNYALLNQTIGEIAERYNVSHETIVVAWILRHPAKIQTIVGSMNPERLK
                     KIAQASQIILTRPEWYDIYRSAGNILP"
     gene            25556..26008
                     /locus_tag="AC1PNG_000059"
     CDS             25556..26008
                     /locus_tag="AC1PNG_000059"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6700605.1"
                     /translation="MQWEIKAFDQLSLQELYTILTLRTDVFVVEQACPYPEIDGKDPS
                     CLHLLGTDNGELVAYLRILPAGLSYDEVSIGRVVIKPSHRGKGLGRPMMEQAIAYITT
                     EWKESQIKIGAQAYLEKFYQSLGFEPISEVYLEDDIPHLDMLYRKLVV"
     gene            26103..27854
                     /gene="aspS"
                     /locus_tag="AC1PNG_000060"
     CDS             26103..27854
                     /gene="aspS"
                     /locus_tag="AC1PNG_000060"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIP6700606.1"
                     /translation="MKRSMYAGRVRKEHVGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPESVEAEVMAKAESLRSEFVIEVTGTVVEREQANDNIPTGAVELQVTSLTV
                     LNTAKTTPFEIKDGIEASDDTRLRYRYLDLRRPEMLNNFKLRAAVTHSIRNYLDELEF
                     IDVETPMLTKSTPEGARDYLVPSRVSKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLNEVEIQDIVEGLIAKVLKDTKGIDVTLPFPRMAY
                     DHAMNFYGSDKPDTRFEMLLQDLTELVKEVDFKVFSEAPVVKAIVVKGAADCYSRKDI
                     DKLTDYAKQFGAKGLAWVKVDKGELAGPVAKFLTGITENLTASLQLEDKDLVLFVADE
                     LEVANNTLGALRNRLAKEQGLIDESKFNFLWIVDWPMFEWSEEEGRYMSAHHPFTLPT
                     EETAHHLDGDLAQVRAVAYDIVLNGYELGGGSLRINQKDMQERMFKALGFSAEDAHEQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEDNIREVIAFPKNNKASDPMTQAPSTV
                     ASAQLEELALNITLENE"
     gene            27841..28779
                     /locus_tag="AC1PNG_000061"
     CDS             27841..28779
                     /locus_tag="AC1PNG_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002265645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIP6700607.1"
                     /translation="MKMNKFFRKQRVRFLRKARKNKFWSVILGISREKYAERISGSII
                     YGLLSSIAVNFFFRPGNVYSSGVTGLAQIVSALAASYAGWNVPIAVVYYGLNIPLLIL
                     AWYKIGYKFTIFTFITVSFSSFFIQFMPPVTLTTDPLVNAIFGGLMLGTGIGFALRNN
                     VSSGGTDIVSIIIRKKTGRQVGSISLIVNIMIMLIAGMTFGWQYALYSMVALFISSRM
                     TDAIFVKQKRMQAMIITNHPERVIKKIHKKLNRGVTIIDGAEGAYNHEQKTILITIIT
                     RAEFNDFKHIMKKADPKAFVSVADNVNIIGRFVESD"
     gene            28856..29308
                     /gene="nrdI"
                     /locus_tag="AC1PNG_000062"
     CDS             28856..29308
                     /gene="nrdI"
                     /locus_tag="AC1PNG_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HIP6700608.1"
                     /translation="MKKKIYLVYISLSGNTESFVKRLKSFFQFQTDWEIELVHVKDLV
                     KQDIPFYQLDAPFVAFLPTYLEGGNGVDNGDVEILTNPLGDFIALGTNAELCLGVVGS
                     GNRNFNNQYCLTAKQYAERFNFPVIDTFELRGMQNDIERIGRKIMELM"
     gene            complement(29462..31513)
                     /gene="pulA"
                     /locus_tag="AC1PNG_000063"
     CDS             complement(29462..31513)
                     /gene="pulA"
                     /locus_tag="AC1PNG_000063"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HIP6700609.1"
                     /translation="MALRQFQASLDDVATIRLVMEKRFDSEHMSFSLESNDATSQLFI
                     HSCLEVDNLVIYYLTSLHALTLDKDYTIYDQDRNKTELGYGHIVRSTLFEQSYTYNGQ
                     DLGATYRPEATTFKLWAPISKQVFLVLEGNPHAMTKGAKGVWEITVEGDLEAHSYHYL
                     HKVNSEWISVHDPYALSSTANSGDSYVINPDKLHKIRRAKTQRPISQAIIYEMSVRDF
                     SWQKEAGFKHRSQFLGLTESPTMDGMKLGMDYIRELGVTHIQLLPVYDFGSVDENKPQ
                     AVYNWGYDPMQYNLPEGSYSSQPNDPYARILELQEAIQAYHDADISVIMDVVYNHVYH
                     AEKYAFELIVPGYFYRYDDHGMRTDGTFCGNDVASERSMVRNYIKQSLRQWLELYGFD
                     GFRFDLMGILDSETINQIQIELSAIHPNVYLYGEGWKMATGLEFEKLAHQYNAEQLPE
                     ISFFNDDYRDTFKKLLLNPNRLVDKQLHEKVQHLLTGSRLSHFLTPQQSLNYIECHDN
                     ATAFDYFHIENPNWTPQQQKRAASFGLQLILISQGMAFIHSGQEFFRTKDEIDNTYNV
                     PDRINRLDWTRAIRYKEHIEFIQELITFRKNNPILSQDNYESIQETCDFYWLTEYVLR
                     YQVTSEEKTIQFIINFADSDFTYEREKDKAVLYNYPPVEERDQQEITIAGQSICILED
                     K"
     gene            complement(31537..32520)
                     /locus_tag="AC1PNG_000064"
     CDS             complement(31537..32520)
                     /locus_tag="AC1PNG_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIP6700610.1"
                     /translation="MRATIKDVAKLAGVSPSTVTRVIQNSSAISQKTKDLVRKAMADL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDVFYQNPFFPTALRGISQVAADHNYAIQIST
                     GKNEEQRLEAISQMVYGKRVDGLIFLYSKPDDPLVQLAIQHKFPFLILGKADSPFISL
                     VDNDNIQAGFEATNYFINKGYKNIAFVAGNKELVVSQDRYTGYKNALKSHNIPLDENK
                     VKFVSGFLLEDSAYKISKKLLKQDLDAIVTTDTSVAEGVVKYLNEVGVKLPIISFDSV
                     KPKLDIEAYVDVHAIKLGRVAFNTLHQIINDNKEDKQVCYRRVIPHTITEL"
     gene            complement(32530..33240)
                     /locus_tag="AC1PNG_000065"
     CDS             complement(32530..33240)
                     /locus_tag="AC1PNG_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700611.1"
                     /translation="MNLWQRLFTTLFLVALLLIPSSLQTVNLETYPLENFVEGVYAPL
                     TDQVVDDLRQYAVIENNHLTYTGNTNWEQVTFGDTVSSSSDFTYQFANQSLTIRKGSD
                     ILSELTYEGLNTDDFASKETLTATISKKWYQANRAAVGLSITLISTFILTTKLLFILL
                     GASFFLFLTKKSRFFHFETFAECYNFALNCLGLPTLIACLIGLFGQPVTTLLTIQNIL
                     FVLCLLWVFFKTKFRDEI"
     gene            complement(33372..34214)
                     /locus_tag="AC1PNG_000066"
     CDS             complement(33372..34214)
                     /locus_tag="AC1PNG_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIP6700612.1"
                     /translation="MKVSVKTRRILNQTLTYLYLIVLSVIIIYPLLITIMSAFKSGNV
                     VAFKLDSNINFTLDNFSKLFSETLYGTWYFNTLIIALLTMVIQTSIVVLAGYAYSRYN
                     FIARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNQSWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPISLDESAKLDGAGHFRRFWQIVLPLVRPMIAVQALWAFMGPFGDYILSKF
                     LLREKEFYTVAVGLQTFISDVKNMKIAYFAAGAILIALPISILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(34216..35511)
                     /locus_tag="AC1PNG_000067"
     CDS             complement(34216..35511)
                     /locus_tag="AC1PNG_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIP6700613.1"
                     /translation="MTMKQNPGKALLLSLIPGLGQIYNKQKAKGYIFLGVTLAFLVYF
                     IAIAAGELGNLITLGSVRGQDNSLFMLIRGSFHLIITVVYLAFYALNLKDARDTAKRW
                     NSGMPVATTLKEMVKGIYENGFPYLLIIPSYIAMTFAIIFPVVVTLFIAFTNYDFKHL
                     PPSALLDWIGITNFTNIWKLSTFRTAFGSVLGWTIIWALCASTAQIVIGILTAIIANQ
                     PFIKGKRIFGVIFLLPWAVPAFVTILTFSNMFNDSVGAINTQVLPFLSKFLPFIDNFL
                     IPWKTDPFWTKVALIMMQAWLGFPYIYVLTLGILQSIPNDLYEAAYIDGASAIQKFRN
                     ITLPMILAVAAPTLISQYTFNFNNFSIIYLFNNGGPGSVGGGAGSTDILISWIYKLTT
                     GTSPQYSMAAAVTLIISMIVISISMIAFKKLNAFEMEDV"
     gene            complement(35724..36983)
                     /locus_tag="AC1PNG_000068"
     CDS             complement(35724..36983)
                     /locus_tag="AC1PNG_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIP6700614.1"
                     /translation="MKHNLLKSVALLAASTAVLAACSNSGSSTEASKSAEGSKELTVY
                     VDQGYESYINDVKAGFEKENGVSVTVKTGDALTGLDNLSLDNQSGSAPDVMMAPYDRV
                     GSLGSEGQLSELTLADDSKADDTTTALVTNGGKVYGSPAVIETLVLYYNKDLLTEAPK
                     TFAELETLAKDSKYAFAGEEGKTSAFLADWTNFYYTYGLLSGYGGYVFGENGTNPKDI
                     GLANEGAIKAIEYAKTWYEKWPQGLQDGTAANNLINTQFTDGKAAAIIEGPWKAASYK
                     EAGVNYGVATIPTLVNGKNYSAFGGGKAWVVPAGAKNQEMAQKFVDFLTATDQQKALY
                     DATNEVPANTEAREYAVSKKDELTTAVINQFASAQPMPNISEMGSVWTPAGNMLFEAA
                     SGSKDAKTAATDAVKAIADEIAQKHSN"
BASE COUNT        10827 a         6680 c         8614 g        10985 t
ORIGIN      
        1 gcacgcttgt actgaccgtg atgtaattaa ctctaataat attatatcac agcggagggc
       61 gctgacttgt gcaaacaact agcaaaaaga aaactaaagg agtttccccg ctggtgcagg
      121 gtggcagtcc tacatcatga ccaaatacag ataggtgatg attggactgt aaagctgttt
      181 gagtttgacc ctgaagacta caagggcaag gtacacggct ggcaacgtga ggcaccaaac
      241 gaggtcaacg agattttgaa agctatcaac gcgatagcta aaccaagaca tcaggctata
      301 cttatcatga gttatatatt gcccgaaaag atacgatcag caaagcaggc acaacgactt
      361 gggatagcag catctactta ctacttggct aaaaatgaag ctttgaaaga gttcgccggt
      421 cagtaccgag atggctcact attgcagtac ttggatagtt aagtttgaaa tcattcgtaa
      481 atacccccca actttttttg aacggggtgc tatatcgttc gaattccaca acgccgccct
      541 cttccgtgta caattttccc tttatgaaac ttttggatac agtaaaaaca tggtataata
      601 cacttatcag caatcaaaaa aagcgtaccg attcgatacg ctagacttgc ctgctgaact
      661 cattattttt agtctatcat gctataaatg ataggctttt tttgttcccc tttttgtaca
      721 ctttctaggg aaatgtaacg ctgtataaag tttacacttt ttttcaaaaa tccagtaaaa
      781 tcaactaaaa aggcgtgtaa agcaaccaaa tgctaaagcc aagcatacct tatcacaaaa
      841 tggtataatg gaaggtacaa tgtttgaaag aggaagacga tgaaattaca agagggagta
      901 gatcttcatt ttattgatac agatcagttt acgacaaatc gtatacgtat tcgctttgca
      961 gctgaaatga gtgaggctac agttgctggt cgtgtgttag ttgcaaatat ttttgaaatg
     1021 ggtaaccaag aatttcagac tgctcaggct gttcggagaa gattggcaga attgtatggt
     1081 gctcagttct cgacctcagt ttcgaaacgt ggtagggtgc actgtgtaga tgtgacaatt
     1141 tcatatgtca gtcctcgtca cttaccagaa aatgaggata ttacagtgga gattcttgat
     1201 tttttataca catgtatatt tagaccactg aaaaaggggc gaggatttga tagccagatt
     1261 ttcgaggttg aaaaaacgaa tttaatcaat tttcttcagt cagagataga agataatttt
     1321 tatcatgcag atgttgaaat gagtaagctt ttttataaag atccctctct tcaaattcca
     1381 cgcgtcggta ggcttgattt ggttgaaaaa gaaacagcag aatcaacttt tcagatttat
     1441 cggaatatgt tgcgtatgga taaaattgat atatttgtct tagggaaggt tgacagagaa
     1501 caagtcaaaa gaaaacttga agattttggt tttacttata gaaatccaaa attagagtta
     1561 gaatataatc aggaatactc aaacatcacg caagaaaaaa tcgagcgtaa acaggcaagg
     1621 cagtccattt tggaattggc atatcattta caagtagttt acaacgatgt aaactatccg
     1681 gctttgatgg tatttaatgg tctactgggt gctttctccc attcgaagtt atttatgaat
     1741 gttcgtgaga aagaaagttt ggcctataca attggcagtc aggtttctat tttttcagga
     1801 atgctgaagg tctatgctgg aattagccat gaaaacagac tcagggtaat gaagttaatt
     1861 agtaaacaac tacttgattt aaaatgtggt aagtttacag aagaagaatt agagttgaca
     1921 aaaaacatgt tgattcattc agcaaccttg gctcaagata ggcagaataa tttgatagaa
     1981 caagtatata atcaagttac cttaggaaat agaaatttaa gttggttaga ttggattgag
     2041 gctatcaaat cggtatcaat agaggatgtc attcgagtag gacagatgat taatttacag
     2101 gctgtttact ttatggaggg aacagaagaa tgaagttagt tgagaagaag tatccttaca
     2161 tgaaagaatc tgtcttttat gggaaagttg acaatgggtt gacagttatt ttgcttccta
     2221 aagtagattt ccatgagaca tatggaattc taacgacaaa ttttgggggt attcatactc
     2281 gatttacatt ggctaatggt aaaagtgttg tttatcctgc agggattgca cattttttag
     2341 aacataagct gtttgaaact gagaatgaag aggatattat gaatgagttt gctaaattgg
     2401 gagcaagcgc aaatgcttat accagtttta gacagactag ttacttattt tcaacgactc
     2461 agaaagtatt ggagtctcta tcacttttac aatcttttgt tcgagaaccg tattttacag
     2521 aagacaatgt agaaagagaa caggggatta ttgaacaaga aattgagatg tatcaagatg
     2581 atgcagatta tcgcttgttt acgggaattt tatcatcgct ttatccagaa agtcctttgg
     2641 cctatgatat agcagggaca gttgaatcaa ttgctgcaat tactgcggat gatttacatg
     2701 agaattttga agtgttttat catccaagta acatgaattt attcgttatt ggtaattttg
     2761 atttagaggc agtttggaaa caaatttcaa gttatcaagt ggctcagatg gataatccag
     2821 ctcagtcgtt tgaattggca ggtgtccaaa agttgccgat acaggaacat ctctcagaac
     2881 aatttgaagt atctacccca aaattggctg ttggtttacg gggaaatgac gaggttgata
     2941 aggagactat tcagaaatat cggcttagtt tacaattttt atttgccatg ttgtttggtt
     3001 ggacttcaaa acgatatcaa cagttatacg agcagggaaa aattgattct tctttccagt
     3061 tccaattaga ggttacacct gaataccact atttgattat ctcgggcgat actcaagaac
     3121 ccattacgct ttctagtata ctgatgaaag cacttaggaa attcgaggat gatgcagatg
     3181 tgacagagga tcatttacaa ttgttgaaaa acgagatgta tggagatttt attcgaagct
     3241 taaattctct tgagtttaca gctagtcact ttgtttctca gtattcagaa tatgaaaatg
     3301 tttttgatat acctcaaatg atacaaagtc ttcagttaga tgacatcaga gaggcaggtc
     3361 gtcgctttat tgatcactgt gatatgacag atttcacgat ttttccaaaa taagtgggcg
     3421 caacccgtcg aaatttcttt ggaattttgt tagaatagta tactgtacat gatgagcgat
     3481 agagagagga tattatgaga caaaagagta taggggaagt acttagaaca gctagagaag
     3541 gacggagttg gacatttgtt gacttgcaac ggataacaaa aatccaagct aaatatttgc
     3601 aggcacttga gtacaatgat tttgatttta ttgcaaactc agatgatgtt cagtctattt
     3661 taaaagcata tgctgaggct ttggaattag atgcagatgt tctactggat gcttacgaga
     3721 ccaatagttt agtgaaatat tatgaagatg gagaagaaga actacgctct tcagaattga
     3781 aaagaagcta taaggttcgt aaacgtaaaa agaactctta ccttccgttg atttacttat
     3841 tacttgcgac gggtttgatt attatttttg tgacttatat tgttcatagc aggctccaaa
     3901 atagagcatc tattacgcct actacaacgt catatagtat agttgagcaa tctgtatctg
     3961 aagcaagtag tggcacagag acgagttcta ctgagcaggc gcagtcaaat tcaacgagtt
     4021 caagtagttc cgttacagaa aatgtgacta tttcaggatc aggttctaac attgttgcga
     4081 cccttaaaac agtgacttat ccagtagaag taactgtaac agcaaaaaat acaacgagtt
     4141 ggatcagcct ttctgacacc ttgttagagg ggggcgttac actaacacct gataatccaa
     4201 cagtaactac tactgttgaa gaaggagtga cttctactac acttattctt ggtgttgtca
     4261 aaggagtaga ggtaactgta ggtggccaaa agctaaatct atcagctctg acagcagata
     4321 caggctcaat tacaataaat tttgaacaat aaaggtttta tatgaaaaaa gaaaatattc
     4381 ccaatgcatt gacgataggt aggattttgg taatcccaat ttttatcttg ttactgaccg
     4441 tttgggattc aacaattagt catgtcttag cagcacttat ttttgcatta gctagtatta
     4501 cggattatct agacggctac ttggctcgta aatggcaggt tgtaacaaac tttggaaaat
     4561 ttgcggatcc gatggcagat aagattttgg tgatgacagc atttatcatg cttgtagagc
     4621 ttggctttgc tccagcttgg gtagttgcaa ttattatttg tcgtgaattg gcagtgacag
     4681 gtcttcgtct actcttggtg gaaaatggag gaacagttct agctgcggct atgccaggaa
     4741 aaatcaaaac attttcacag atgtttgcag ttattttcct gctcttacac tggaacgccg
     4801 tggggcaaat aacactctat attgcacttt tctttaccct atattcaggt tatgattatt
     4861 tcaagggtgc gtccttcttg tttaaagata catttaaatg atgaatatta ttgaagtaaa
     4921 aaatttaaaa tataaataca accaagagga tgagcgctat actctaaatg atgtttcgtt
     4981 tcacgtgaaa cagggagagt ggctgtctat tattggtcat aatggttcgg gtaaatcaac
     5041 aacagttcgt ttgattgatg gtttgttgga agctgaatca ggtgatattt atattgacgg
     5101 tgatgcctta accattgata atgtatggga taagcgacgc ttgattggta tggtttttca
     5161 aaatccagat aaccaatttg taggcgctac agttgaagat gatgttgcat ttggtttaga
     5221 gaatcaggga atccctttgg aggagatgcg tagtcgagtt gacgaagctt tggaattagt
     5281 tggaatgact gatttcaaaa cacgtgagcc tgctcgttta tctggtggtc agaagcagag
     5341 ggtggcgatt gctggagtgg ttgccttgcg tccaaaaatt attattttgg atgaggctac
     5401 gtctatgttg gatccagaag gtcgtctcga cttgattaag attgtccgtg aaattaagga
     5461 gcgtcatggt atgacggtta tttctataac acatgatttg gatgaggttg cattgagtga
     5521 tcgagttatc gtgatgaaaa atggacaggt tgaatcaatt agcactccaa atgaactatt
     5581 tatgcgagaa gatttggtag atttagactt ggatagaccg tttacgacag aactggcttc
     5641 atctttgcgc cagactggac ttgatttacc gctccgctat tttacagagg aagaattaga
     5701 ggaaacttta tgggaattga tctccaagaa gtaagctata cctatcaagc tggcactcct
     5761 tttgaggggc gtgcgctttt tggtgtggat ttggagattg ttgatggctc ttacacggcc
     5821 cttattgggc atacgggttc gggcaaatca acgattttac aactcttgaa tggattaaat
     5881 gtaccaacag aaggaagagt agtcattgat gatgttgtga ttacagcgac gtctgaaaat
     5941 aaggatatta agcaagtcag gaagaaagtg gggctggtgt tccaatttcc tgaaagtcaa
     6001 gtgtttgatg aaacggtctt aaaagacgtt gcttttggtc cacaaaattt cggagtttct
     6061 caggaagagg ctgaaacgat tgcgcgtgaa aagttggcct tggttggaat tgatgaaagc
     6121 ttgtttgagc gtagtccttt tgaactatct ggtggtcaaa tgcgtcgggt agctattgct
     6181 gggattctgg caatggaacc aagtatctta gttttggatg aaccaacggc tggattggat
     6241 cctgcgggtc gtcaggaatt gatggatatt ttcaaaaagc ttcatgaatc aggcatgaca
     6301 atcgttttgg taactcattt gatggacgat gtggctaact atgctgattt tgtttacatt
     6361 atggaaaagg gaaagctggt ccgttctggt aaaccagtcg atatttttca agaggtagat
     6421 tttttagaga gtattcagtt gggagtgcct aagattacta agtttgcagc taatttggag
     6481 agaagaggtt ttgtacttga acgtcttcct attacaattg aagaatttac ggggatggtg
     6541 acacatggat aaattaattt taggtcgcta tattcctggg gattcaatta ttcatcgttt
     6601 ggatccaaga agtaagcttt tagcaatgtt tggatttcta ttgatgattt tttgggccaa
     6661 taatttggtc accaatgcct tgttgatagc atttgtttta ggaatggttg ttttatctcg
     6721 tattcgctta tcatttttta ttaatggttt gaaaccgatg ataggaatta ttctatttac
     6781 aactttcttc caggttttct ttacacctgg agcgaccatt ctatgggaat tttggatttt
     6841 taaattatct gtagaagggt tgcaacaggc ggcgattatt tttgttcgct ttgttttgat
     6901 tattttcttc tcaaccctat tgaccttgac gacgacaccg ctcagtttgg ctgatggtat
     6961 tgagtcgggg cttgcacctt tgaagaaatt taaagttcct gtgcatgaaa ttggtttgat
     7021 gttgtccatg agcttgcgtt ttgtaccgac cttgatggat gataccactc ggattatgaa
     7081 tgctcagaga gctcgtggag tggactttaa tgagggaaat cttattcaga aggtaaagtc
     7141 agttattcct attttgattc cgttattcgc atcaagtttt aagagggcag atgctcttgc
     7201 aacggcgatg gaggcgcgtg ggtatcaagg cggagatggg agaaccaagt accggatttt
     7261 ggaatggaag ttagcagata cgttagcaat tttagttatg ttagctttag ctggactctt
     7321 atttatattg aaaaaataag taaaaaacgc tagaaatagc gtttttattg tctaatctca
     7381 atttgtctgt aatatttgta acaatttcac aaagtctaag taatagttag agggtatgat
     7441 agacacatga cagaaaggaa tgtgactatt atgaacatta cattaaagaa aaacttaaaa
     7501 acaactattg ctggtttggt agcgggaatc tcccttttaa ctgcgggtgt agtcagtgca
     7561 gaaacctata ctgtaaagtc tggtgatacc ctgtctgaaa ttgctgaaac atacaatact
     7621 actgttgaaa aattagcaga gcagaataag attaccaact tagattttat ccatgtcggt
     7681 caagttatcg aattgggaga tgtgacagtg aaggcagtag agagttcaat tgaacaaact
     7741 caatcaacat caacttctac tgcaacagta acaactagca cctattcttc aaatttgagt
     7801 gctgaagatg cagcagcaaa agaatggatt gcaatgaagg aatcaagcgg tagctatgat
     7861 gcccgtaacg gtatctacat tggtcgttac cagttgacaa atacttactt gaatggtgac
     7921 tactcaccag aaaaccaaga gcgtgtagca gatgcttatg ttgctagtcg ctatggttca
     7981 tggtctgcag ccaaggcttt ctggttggca aatggttggt actaagataa attagaagag
     8041 gtctagcacg atgctagact ttttctgtct cacgaatgta tttcgcaact gctttttcgt
     8101 tcagtccaac ggtactgaat agtaacctct agcccaaaac ttttgatttc cacgctgtat
     8161 ggacataggt gatggaaaat atcaattaag tctagtctaa ttttttagta aatggatttt
     8221 ctgcggtact taggagtgac atacatttgg tatgtgataa actgtaacgg ttttaaccat
     8281 gctaagctag tccactggac tagtttactc ctcctttatt taacttgaca aattagatta
     8341 taacaggaaa aaaatagaag ctcaaagcct tgccagccac cagtataggt ggtatttttc
     8401 ttgtttttct ctatgagaaa actagcccga agccataata agaaaaagga ccgttagggt
     8461 cctttttggt ttacattatt gtcaatcttc cccaaaaatt tcttgagtaa ggcgacgacc
     8521 cgtcggtgta gctgcaaggc caccttcagc agtttcacgg aaggcagttg ggagggcggc
     8581 tccaacttgg tacatggcat tgataacttc atccacaggg atttttgatt caattcctgc
     8641 cagtgccata tcagcagcca ccatagcgta gctagctccc atggcgtttc gtttgacaca
     8701 tgggacttct accagaccag cgacgggatc gcaaatcaga cccagcatat tttttatgac
     8761 aaagcagatg gcttggctgg cttggtaggg agttccacct gctgccagtg tcagggctgc
     8821 ggcagacata gcagaggcag atccgacttc tgcctgacag cctccttctg cacctgagat
     8881 ggaggcgttg ttggcgataa ctagtccaaa ggctccagct gtaaatagga agtcaagttg
     8941 ctgttcttct gtcaaattga gcttttcaat agcaactcct agcacagctg gtagacagcc
     9001 tgcagatcct gctgtcggag tggcacagac taagcccatt ttggcattca attcattaac
     9061 ggccatggca tttcttgcgg cggataggac agcggaatct gacagggttt taccagattt
     9121 catataggca tctaacttgg cagcatcgcc tcctgttaaa ccagatacag atttactttc
     9181 agtaagtcca tctacgatgg aggctttcat aactgtcaag tttcttgaca taattgtcaa
     9241 tatttcttcg cggctacgac cggtcaactc aatttcagtc gcgatcatga gttcagctac
     9301 attagcagaa aagttgtctg cttgctgaac taattcttcg atagtataaa acatgtcttt
     9361 ctctttctag ttgaagaagg ttacattgtg caggtgtgga attttttcaa tttctgcaat
     9421 agaattttca cattggcgag catccacttc gataatcata atggcttttt cgccagcttt
     9481 ttcacgtgtc acgttcattt gggcgatatt gatgtcatac ttagaaagga catcggtcac
     9541 tttcgcaatc ataccaggga catcttggtg aacaataata atagttggcg tattcatatt
     9601 gagattgaca gagaagccgt tgagctctgt tacttgaatg tttcccccac cgatggaaac
     9661 gcctgtggct gagatggatt ttttatcgtt ttttatgatg attctggtgg tattgggatg
     9721 tggggtattg ctgtccttgt tgacacgcca gtaaacctta atgcctcgtt ctcttgcaat
     9781 atcaagagag tcaggaatgc ggggatcatc cgtatccatg cccagaatac ctgctaccaa
     9841 ggctacatcc gtaccgtgac cacgataggt cttggcaaag gaattgtaga gttgaaattc
     9901 cacctctgtc ggctcatcgc cgaagatgga agatacaatt ttcccaattc ggaccgcccc
     9961 agctgtgtga ctagaagatg gaccgatcat aacaggtcca ataatatcaa agaccgactg
    10021 aaattttaag tttgtcatag taatcctcac gtttttcttg gtactattat atcaaatttt
    10081 ctagttagag aatatgccta gatgaatgtg ttttcttatg ttaaaattct atcgcttacc
    10141 ctttttgtat ggtataatac aagttgttca aaatcgtcaa tcgtttttgg ataatcaaat
    10201 tttggaatac gaccttcaaa tcgttatttc tacgaaaaga tgggagaaga ttatgtcaat
    10261 tgacaattct aaaacccgtg ttgttgtcgg tatgagtggc ggtgtggatt catcggttac
    10321 ggctctcttg ctcaaagagc agggctacga tgtgatcggc atcttcatga aaaactggga
    10381 cgacacggat gaaaatggct tctgtacagc aacagaagat tacaaggatg tggctgctgt
    10441 tgcagaccag atcggcattc cttattactc tgtcaacttt gaaaaagagt attgggatcg
    10501 cgtatttgag tactttttag cagagtatcg ggcaggtcgc acacccaatc cagatgtcat
    10561 gtgtaacaag gaaatcaagt tcaaggcttt cttggattac gctatgaacc tgggtgcgga
    10621 ctatgtggcg acagggcact acgctcaggt gtcacgcgac gaggacggca ccgttcatat
    10681 gctgcgtggg gcagataatg gtaaggacca gacctacttc ctcagccaac tctcacagga
    10741 acagctgcag aaaaccatgt ttccgctcgg ccatttacaa aagcctcagg tgagggaaat
    10801 agcagagcga gcaggcttgg cgacagctaa aaagaaagac tcgacaggca tctgctttat
    10861 cggtgaaaag aacttcaaag aatttctagg gcagtatttg ccagcccagc ccggtcggat
    10921 gatgaccgtt gatggtcgtg atatgggtga acacacaggc cttatgtact ataccattgg
    10981 ccagcggggc gggcttggta tcggaggaca aatcggtggg gacaatgagc cttggtttgt
    11041 tgtcggaaaa gacctgtcaa aaaatatcct ctatgtcggt cagggcttct atcatgagtc
    11101 cttgatgtca acttctttac aggctagtca agtccacttt acacgtgata tgcctgaaga
    11161 attcaagctg gaatgcacag ctaagttccg ctaccgtcaa ccagacagtc aagtaactgt
    11221 caaggtgaaa ggtgacaagg cagaagtcat ttttgcagag ccacaacgag caatcacacc
    11281 aggacaagcc gttgttttct acgacggaca agagtgtctg ggcggtggta tgattgatat
    11341 ggcctataaa gatggagagg cttgtcagta tatttaatca aaaataaaag cttagcaccc
    11401 ttgtgctgag ctttttatct tatccccaga aagcatcttc ggcggtttgg tcggctgaga
    11461 agagccaaac ttccttgatt ttgccatctt ccatacggaa aagatcgatg ccattcatgt
    11521 ttagttcttc accgtctgtt cgagtaccga ggaatgttac gttagcggca acaagagtat
    11581 cattatcgga tacccagttt gtcactactt tgaatgttcc atttgttttt tcggcgaaag
    11641 ttgctagatg tgctccgagt acttccttac caattttagc accagatgtt gaatgatttc
    11701 cgggttgatg ccagataatg tcgtctgcca ttgtttcaaa aagagctgca aaatcaccag
    11761 cgatgagagc attgttatag gcattaaaaa tttccaagtt tgttgacatt tgatgtcctc
    11821 caattttttt gatatagtac aattatagtg gaagtaatca attatacaag taggtacttt
    11881 tgcatttgtt ggtatcaaaa gtgatactat tgtgataaat aaaggagaat ttttttgaaa
    11941 aaagttagtg aatatggtat ttgccctttt gcaacaacac aaaaggtctt gactggtaag
    12001 tggggattgg tgattatcta ccaactcagt acaggaacga aacgcttcaa tgaattacag
    12061 cgactgattc caggtatcac tcaaaccatg ttaacaaggc accttagaca attggaggag
    12121 gatcgcatta ttagtcgcac ggtctatgca gaagtccccc ctcgtgtaga gtatagcttg
    12181 actgaaatgg gcgaaaaatt tcgttcggtg ctggatgctg ttgaggcttg gggcttagaa
    12241 tatattgaaa cgttaacgta acaccagatt tgaaattcta caaatctttt gctaaaataa
    12301 gagtaacaat catcatgatg gtcaaagctc atgggaattg taggcttttt tgtcctgcaa
    12361 ttttcgagag ttttggctat tttttgctat tggggagaga agatggattt cagaaccaaa
    12421 gttgacaacc agattttcgg tgtccgtgcc actgcattgc ttataaaaga cgggaaaata
    12481 tttctcacta aagactctaa ggggcgttat tataccattg gcggtgcggt tgaggtcaat
    12541 gaggtagcag cagatgcggt tgttcgagaa gtcaaagaag aattagggat tgacagtcat
    12601 gtcaaccaac tggcttttgt tgtcgaaaat aagtttacac aggaaggcat acactttcac
    12661 aacatcgagt ttcattttat cgtggagcct attggagaaa tacctgagga gatgattgaa
    12721 gataacttga aacaagcttg tgaatggata gacgttgaca aacttgtcaa cctagatgtc
    12781 gtaccagttt ttctggcaga gaaattgcca aactggagtg gacaagttac acacattatg
    12841 aacatgaagg agaaaacaac atgacacaca catttgcaga aaattatgat gtcatcgtga
    12901 ttggtgcggg gcatgcgggt gtagaagcag gattggcagc cagtcggatg ggctgtaaga
    12961 ccttgcttgc aactattaac ttggatatgg tggcctttat gccttgtaat ccctccattg
    13021 gtggctctgc caaggggatt gtggtacgag aaattgatgc cttgggtggc gaaatgggtc
    13081 gcaacattga caagacctat atccagatga aaatgcttaa tatgggtaaa gggccagctg
    13141 ttcgtgcctt gcgggctcag gcagataagg cagagtacgc agcagagatg aagcggacgg
    13201 tggagcgtca ggaaaatctg accctgcgtc aaaccatgat tgatgagatt ttagtggaag
    13261 acggcaaggt tatcggtgtc cgcacggcta ccaaccagaa attttcagcc aaggcggttg
    13321 tggtgacgac gggtacagcc ttgcgtggtg agattatcat cggtgacctc aagtattcat
    13381 caggacctaa caatagtctg gcttctatca cgctggcgga taatctgaaa gaattgggac
    13441 ttgaaatcgg tcgtttcaag acaggaacac caccgcgtgt caatgctcgt accattaact
    13501 acgaagaaac cgagattcag ccagacgatg aaaagccaaa tcacttttca ttcttgtcca
    13561 aggacgagga ttatttgcag gatcagattc cttgctggtt gacctatact aacgcgacca
    13621 gccatgagat tatcaacagc aatcttcacc gagcacccat gttttcgggc attgtcaagg
    13681 ggattggtcc ccgttattgc ccttctattg aggacaagat tgtccgtttt gccgacaagg
    13741 aacgccacca gcttttccta gagccagaag gtcgcaatac agacgaaatc tatgtccaag
    13801 ggctgtcaac cagtctgcca gaagatgtgc agcaggactt gatccactcc attaaaggtt
    13861 tggaaaatgc ccaaatgatg cggacaggct atgccattga gtacgatatg gttatgcctc
    13921 atcagttgcg ggcaacgctt gaaaccaaga agatttcagg gctctttaca gcaggtcaga
    13981 ccaacggaac atcaggttat gaagaagcag caggtcaggg aattgtcgct ggtatcaatg
    14041 cagccctcaa ggtgcagggc aagcctgagc tgattttgaa acgaagcgac ggttatatcg
    14101 gcgttatgat tgatgacctg gtaaccaagg gaacggtgga gccttaccgc ctcttgacca
    14161 gtcgggcaga ataccgcttg attctgcgtc atgacaatgc tgatatgcga ctgacggaga
    14221 ttggtcgtca ggttggcttg gtagatgatg aacgctggca ggtcttccaa atccataaaa
    14281 accagtttga caatgaaatg aagcgcttgg agtctatcaa actcaaacca atcaaggaaa
    14341 ccaatgaaaa ggttgttgcc atgggcttca agcctttgac agacgctctg actgccaagg
    14401 aattcatgcg tcgtccagat gtgacttacg cagatgcggt agcctttatc ggtcctgcgg
    14461 cagaggattt ggacgccaag accattgaat tgattgaaac agaggtcaag tacgagggct
    14521 acattgccaa ggccttggat caggtggaga agatgaaacg tatggaagaa aaacgcattc
    14581 cagccgacat tgactgggat gatattgact ccattgcgac cgaggctcgc cagaaattca
    14641 agctgatttc accagaaacc attggtcagg ctagtcggat ttccggtgtc aatccagcgg
    14701 atatatccat tctcatggtt tacttggaag gacgtagtcg ttccatttct aaaaacaaat
    14761 caaaggacag tcactaaggc tgttcttttc gctgtatgtc agctatatgt caactacttt
    14821 aaaatcatgt gaaaagtagc ttttttactg aatttatggt agaatggcag gcagaggtta
    14881 gtgatgaaaa gatttcgatt ttcgacaatt cactttgtca tgataggtgt cattttattt
    14941 gggttggtgg ctcttgtcca ccgatttttt ccaattggtg ccagtacagt ttttgcgcta
    15001 ctcatcagcc tattggtgtt gattgcgctg tttatttatc aaaaacattc ttatgaattt
    15061 agtgagctag aacagattga atatctcaat aatcaggcaa attctggttt gatgatgctc
    15121 ttggataaaa tgccagtggg agttatcaaa gttcgaccag atagtaatca ggtagaatgg
    15181 tttaatccct ttgctgagct gatttttgct caagaaaatg gtgagtttga tcaaaaaaaa
    15241 ctacgtgaaa ttattgacgt tggtctggat gaggagcgaa tttatgcaaa cttttcaggc
    15301 aagagatacg ctgttaacgt tgattttgat caagggttat tttacttttt tgatgcgtcg
    15361 acagagtatt cagcgacaaa tagtttgatc ggaagccgtc ctgttatagg gattatttca
    15421 gttgataact atgatgagct tgaggatgct gtaactgatt ctcaaattag ccaagtcaat
    15481 tccttcttgg ctcaatttgt ttcggaattt tgccatgatt acggtatcta ctaccgacgc
    15541 ggcacaatgg atcgattcta tttctttaca gattatgctg tgttggaaaa attgatagag
    15601 aataagtttt cagttattga taagttccgc gaggaggcta aaaagttaga tttaggtttg
    15661 actttgagta tgggattggc gtatggtaat gaaaatcatc accaaatcgg acaggttgcg
    15721 ctccaaaatc tcaatatggc tgaggttcgt ggtggagacc aggttgtggt caaggaaaat
    15781 gatgagagta aacagccact tttctttggc ggtggctcgg cttcttccgt taaacgaaca
    15841 cgtacgcgga ctcgtgctat gatgactgct gtatcagata aactcaaatc ggttgatgct
    15901 gtttttgtgg tgggacatcg taatttagat atggacgcgc ttggatccgc ggtcggcatg
    15961 cagtactttg ctcaaaatat tatgaacaat gcctatacgg tgtataatcc agatgaaatg
    16021 gcaccagata ttgcacgtgc catcaagaag ttgagtgatg aaaattgttc taatttgcta
    16081 acggttgagg aagccatgaa gatggtcact ttccagtcgt tgttaatcat ggtagatcat
    16141 tcaaagattg gcttgacttt ggataaggat ttctatgacc aatttaccca ggttgtggtg
    16201 gttgatcatc atcgtcgcga tacagatttc ccaaataatg cagttttgac ttatatagag
    16261 agtggtgcta gttctgcgag tgagttagta acggaattaa ttcagttcca gaatgacaaa
    16321 caccataagc ttaatcgcat tcaatctagt ttattgatgg ctgggattat gcttgataca
    16381 aaaaatttca cctctcgtgt gactagtcgt acttttgatg tggctagtta cttgcggact
    16441 cgtggtagcg atagcttgga aatccagcag attgctgcga cagattttga ggaatatcgt
    16501 caaatcaatg aactgatttt aagcgggcag agaattgagg ccaatgttgt tattgcgtgt
    16561 gcagatgata ctaaagaata cgataatatt attccgagta aggctgccaa tacaatattg
    16621 gacatggcag gaattgaggc ggtattcgtt gtaacaagaa ataccaaagg gtatgtatct
    16681 atttctgcaa ggagtcatag taaaatcaat gttcaacgca tcatggaaga aatgggtgga
    16741 ggtggccact ttaacctagc tgccgctcag gtatatgacc agacaatcgc agaagtatgt
    16801 gaaaagttga cggataaaat ccgtgaagaa ataaatggaa attaggagaa cagatatgaa
    16861 agtaatcttt ttagcagatg ttaaaggtca aggtaaaaag ggtgagatta aggaagttcc
    16921 aacaggttac gcccaaaatt tcttgatcaa gaaaaatttg gccaaggaag caacaaacca
    16981 agcaatcagc gcccttcgtg gtcaggaaaa atctaaggaa aaggctcatg cagagatgat
    17041 tgcagaagca gaggctatca aggctaagtt ggctgaggaa gctactttag tgaaatttgt
    17101 cgaaaaagtt ggtccagatg gccgtacctt tggttctatt accagcaaga aaattgctga
    17161 agaattggac aagcaatttg gcatcaagat tgataaacgc catattcagc tggatcaccc
    17221 aattcgtgcg attggtctca ttgatgtacc agttaaaatc tatcaagatg taataggcat
    17281 cattaatttg agtattaagg aagcttaatt atttatctag ccagtgagga ggtgagatct
    17341 tggcagagtt ggatgaattg cgggtgcaac cgcaggatat tctagctgaa caatcggtat
    17401 taggagctat tttccttgat gaaggcaagc tggtcttcgt tcgtgaatac atcgaagctg
    17461 aagatttttt caaacatgct cataagctaa tttttaaagc tatgattgct ctttctgata
    17521 gaaatgaggc aattgatgcg actaccatgc gtaactattt agtgaaccat ggtgatttgg
    17581 aaaatatcgg tggtttggcc tatttggctg aaatcattag ttccgtgccg acggcagcca
    17641 atgcggaatt ctatgccaag attgttgctg aaaagtctaa tctccgtaaa ctgattgccc
    17701 gtttgacgga tgcagttaat caggcttatg agggtgcgga tggttcagat gacattattg
    17761 ccaatgctga aaaagctttg attgatgtca gtgagggagc cagtcgaagt ggtttcaagc
    17821 ggattgatga tgtcttgaat atcaactttg acaaccttga aactcgtgct aaacagacgt
    17881 cggatattac agggattgca actggctatc ctgctcttga tgctatgaca acgggtctgc
    17941 atgaggaaga gttgattatt cttgcggctc gtcctgcggt tggtaagacg gcttttgccc
    18001 tcaatattgc tcaaaatatc ggtacaaaac tgggtttgac agttgccatc ttttctttgg
    18061 agatgggtgc agaaagcttg gttgaccgta tgctcgcttc agagggagtt atcaattcga
    18121 gggctattcg tacggggcat ttgacgcagg aagaatggaa taagtacatg gtggctcaag
    18181 ctaacctggc tcgggctagt atctatattg atgatacacc aggtatcaag attacagaga
    18241 ttcgttcgcg ttcacggaag ttggctcagg aaacgggcaa tctgggtttg atcttgattg
    18301 actatcttca attaattacg ggaacaggtc gggaaaaccg acaacaagag gtttctgaga
    18361 tttcccgtca gttgaagatt ttggccaagg aattgaaagt tcctgttatc gccctcagtc
    18421 agttgtcgcg tggtgtggaa cagcgtcagg ataagcgacc agttttgtct gatattcgtg
    18481 aatctggatc tatcgagcag gatgcggata tcgttgcctt cctgtatcgt gatgattatt
    18541 atgagcgcgg tgagcaggaa gacggtggta ttcccaataa tacggttgaa gttatcatcg
    18601 agaaaaaccg ttccggtgcg cgtggtaccg tggaactgat gttccaaaaa gaatacaata
    18661 aatttgcaag tatttccaag agggaggatg gatagagaat gagtgatgca tttacagatg
    18721 ttgctaaaat gaagcaaatc aaacaagata ttaaagacca tgagggacag attgtcgagt
    18781 tgacccttga aaatggtcgt aagcgtgaga agaataagca ggggcgtatt gtagaggttt
    18841 atcagtctct ctttatcgtt gaatttgaag atccaaacaa tacctttgtg gaatcttata
    18901 cttactcaga cattttgaca gaaaagattt tgattcatta tttggattaa tcgctatata
    18961 aggctttaca gagccttttt tttgtgctat actgatattt gtgtgaaata acagcagggc
    19021 aaaatgaagc tcgtcaacag aaggtcagca agaagagtaa tcgttgctgt ttcgatgaag
    19081 gcctcctgca cgaatcaggt ttgcttaaaa cgttatttcc tacaaataat ttttatattt
    19141 tattttagga ggacataact atgtcacgtt atacaggacc atcttggaaa caagctcgtc
    19201 gtcttggctt gtcattgaca ggtacaggta aagaattagc acgtcgtaac tacgtaccag
    19261 gtcagcacgg accaaacaac cgttctaaat tgtcagaata cggtttgcaa ttggctgaga
    19321 aacaaaaatt gcgtttctct tacggtttgg gtgaaaaaca attccgtaac ttgttcgtac
    19381 aagctactaa aatcaaacaa ggtactcttg gtttcaactt catgcttctt ttggagtgtc
    19441 gtttggacaa cgttgtttac cgtcttggtt tggcaactac tcgtcgtcaa gcacgtcaat
    19501 tcgtaaacca cggtcatatc cttgttgacg gcaaacgcgt tgacatccct tcataccgcg
    19561 ttgaagttgg tcaagtgatc tcagttcgtg agaagtcaat caaagttcca gctatccttg
    19621 aagctgttga agcaactctt ggccgtccag ctttcgtatc attcgatgct gaaaagcttg
    19681 aaggttcatt gactcgcttg ccagaacgcg acgaaatcaa cccagaaatc aacgaagctc
    19741 ttgtcgtaga attctacaac aaaatgctct aagatttgta tcgataattt aaaagcccta
    19801 tttacaaggg ctttttgtct gtatttgagg aggggattat gtggcgtaag tattttttac
    19861 tagtgctatt agctattttg gtcgtaggtg gggccttctt tctatctcaa tcagcggcta
    19921 cgccaaatga atcagactat gtgaaggctg taaagagtta ccaggctatt tcgatagatg
    19981 aagtggagca aaaggtccaa gacgaggaag aatttatatt atacataggt cgtgagacat
    20041 gcccttattg tcgtgatttt gttccgaaat taacagaggc agtagagcga tcacgtgcga
    20101 ctatctacta tttggatagc gagtcggatc cgaatggaaa aatacaacaa tttcgacaga
    20161 gtcaaggtct tgctacggtt cctagcctaa cctattataa atcgggtaaa ttatctggaa
    20221 tacttcgtaa agggagtcaa gcaacgctat ctgaaataga gaatttttta gctcttcaag
    20281 ggcagtaaat agttttaaga atgtatgaag cgaatatcat acattttttt gttcatgttt
    20341 gtgaaaatat ttcaaaacag attgaaaaac atttcacaat cttgtataat actaatatca
    20401 gataaaaatt ggaggtttat atgaaaaaga aagcttggtt aggcttatta atgagtctac
    20461 ttgctgtttt gtttttagtg gcgtgtggag gtaagtctga aaagacagct acatcttcat
    20521 catctacgac ttcttcttca tcagaagaag ctgtttcagg tgcatctgag aaagtttata
    20581 cagatccatc tgagttgaaa gatagctacg atgttatcgt tgttggttca ggtggtgcag
    20641 gtatgtcagc agctatctca gctaaagacg ctggtgctag tgttgctctc ttggagaaaa
    20701 tgcctgttat tggtggtaat acggctaaat catcagctgg tatgaatgct tcacagacta
    20761 aattccaaga agcggaaggt attgctgata caaacgataa attctacgaa gaaacgctta
    20821 aaggtggtaa agggacaaac gatcctgaat tgcttcgtta tcttgtagat aactctgcta
    20881 gtgcaattga ctggttggat ggaatgggga tcactcttag caacctaact acaactggtg
    20941 gtatgagcga gaaacgtacc caccgtccag cagatggttc agctgttggt ggctacttgg
    21001 taaatggtct ttaccacaat cttgttgaac gtaaagtacc aatttttgtc aatgcagatg
    21061 ttacaaaact ccaagacaag gatggagcag tgacaggtgt tgaagtgaag attgctggtg
    21121 aaacgaagca aatctcagct aaagcagttg tattggcgac aggtggtttc ggtgctgatt
    21181 tggaaatggt tgctaaatta aacccagcat tgaaaggtta tgtaacaacc aaccaagaag
    21241 gttcaacagg tgacggtatt gcacttgctg aatcagaagg tgcagcaact gttgatatgg
    21301 aacaaatcca aattcaccca agtgttgagc aagaaacttc ttacttgatt acagaagcag
    21361 ttcgtggcga aggtgctatc cttgtcaact caaaaggtga gcgttttgtt aacgaattgg
    21421 aaactcgtga taaagtttcg gcggctatca atagtttgga tccaaactat gcttatgtta
    21481 tctttgatga tgccttgaaa gatcgtgtga aggctattgc tcaatacgaa gaaaaaggtc
    21541 ttgtaaaaac gggtgctaca cttgcagatt tggctaaaga aattgaccta cctgcggaca
    21601 cacttcaaac aactcttgat acttggaaca aggctgtggc agataagaat gatgcggcat
    21661 ttggtcgtac atcaggtatg gaccatgctt tgaacactgg ttcttaccat gccattaaag
    21721 ttgcacctgg tgtccaccat acaatgggtg gtgtgaaaat caataccaat actgaagtat
    21781 tgaaaacaga cggttcagca atttctggtc tttatgctgc tggtgaagta actggtggtg
    21841 ttcatggtca aaaccgtatt ggtggtaatg cagtagcaga catcatcgta tttggtcgtc
    21901 aggctggtga acaagcagct ggatttgcta agaaataaat tgtagacata agaggctcgg
    21961 cagaaagaag gtcgagcttc tttttcttgt ccctctatct ctaagatgat atacttttaa
    22021 ggtatgaaga agaaaatagt attattgtta ggagctattg cagttatagt tttgacttgg
    22081 tctgctattg ctggtcagcg tcagcaatct tatgccgagg ctgaattaag ggaccaggat
    22141 aaaatctgga ttatcactga tttgcattat ttgtcacagg atttatttga tgatggagag
    22201 gcattttctt atatcgaaaa gacagctgct ggcaaggatt tacgttatgg aaaagagcgg
    22261 atggaggctc tggttgagca ggttgggcgt gaacatcctt ccttgttact agtcagtgga
    22321 gatttgactc ttaatggaga aaagcagagc atggttgaat tagctcagta ttttacagag
    22381 attgaagaac aaggaactga ggttttggtc attcctggca accatgatat tgccagtgga
    22441 tgggctaggg cctttaaggg agaccagcag atagtgacgg atcaggtcac cgctcagcaa
    22501 tttgcagagc tatttgccca tcacggttat caacaagcta gcagtcgtga caaagcctca
    22561 ttgagctacc tggccaaacc attttcaaat gcttggtttc tgatgataga cagcaatatc
    22621 tactctgacg gctatggaaa aggagctcct ccaaccaatg gacgtatcaa taaagagact
    22681 ctagaatgga tagaaattca gcttcaagca gccaaggaaa ctggagtgag cctgatacct
    22741 gttgtccatc acaatgtctt gcaacagcat gccatgcttt caaaaggcta tactttggat
    22801 aatgctgctg atttgaagac cttgttcaat caatatggca ttcaatttgg tttttcaggg
    22861 cacactcatt ctcagaatat tgttaaggaa gacttgggac aagttaacta tactgaagta
    22921 gtcaatggag cattttctat ttatccagct attatcggcc aattgagcct tgatgataca
    22981 agcattcatt atcaaaaaac tcaacttgat atggcttcgt gggcagataa gcaccaacct
    23041 agtgatccaa atttgcagga tcacgttacc tacttacaaa ctgtctttga caataccagt
    23101 gacatcatgg tccacaatat gttaaacgat gaaggttggt atgatggtga aacagcagat
    23161 gctatttcac aatttatcat gccggccaac cgtgcttatt tttcaggaga aaaactagat
    23221 cagacttggc tagatgagaa ggtatttcca agccaggctt atcagacctt gcaagcagca
    23281 tcaccgagaa gtttcttggc tgattatctt gatgtgatta taaaacggag tcaaaatagg
    23341 gatgtagaaa aactagtgct taaaacagaa aaagattttg tccaatagtt ttccacagat
    23401 tgttgaaaaa aaccacaact tatccacaag ctgtgaataa gttatgccgt agtaactatc
    23461 cgagtaggtt tgtttttaca aacaaaaata ctcagcaact ttcctgaaaa tgtgataaaa
    23521 tagtaacaaa ttgtgtcaaa tggagagaaa gatgacctta ctaagcattg ctattccgag
    23581 ttataattcg gaggcttacc ttcattactg cgttcattcc cttgtaatgg gtggggacaa
    23641 ggttgaaatt ctgattatta acgatggttc gtcagaccga actcaggaaa tcgcagagga
    23701 gcttgccaat caattttcaa atgtacgggc tatttatcaa gaaaataaag gtcatggcgg
    23761 agctgtcaat acagggatac gtgaggccaa ggggaagtac tttaaagttg tagattcaga
    23821 tgactgggtc gataccaggg cctatttgaa gatcctagag agtttgcagg ctttggaaga
    23881 tgaaaatacg ccagttgatg cctttatctc caactttgtt tacgaaaaag agggacaatc
    23941 tcgcaaaaaa tccatgtctt atcaatccac tctacctgag aaccgtatct tcggttggga
    24001 agacgtgggg gcattttcca aggggcaata catgatgatg cattccttga tttacaggac
    24061 agatttattg cgtgaggttg gtctcgttct gccagagcat actttttatg tggataatat
    24121 tttcgtattt actcctttgc aggctgtgaa aacgatgttc tatctgccag tagattttta
    24181 tcgttacttt attgggcgaa acgaccagtc tgtcaatgag tccgtcatga ttaagcgcat
    24241 tgaccagcag ttaaaagtca atcgaatctt ggtagataat ttagacttgg aggcgattga
    24301 caatcctgat ttacgaagtt atttgctcaa tcatgtggag attacgacca ttatctcctg
    24361 tgcccttctc aatcgtgcag gaacagctga gcatatgatg aaaaagcagg aattatggca
    24421 ctatattcgg gataacaatc cagctctctt taaaattgtt cgtaaagggt tactcgggca
    24481 attaaccaac ctttacggtt acccaggtcg aaaaattttt aatgccgtct ataaaatcgc
    24541 gaaaagaatt tatggtttta actaaaactc agtttcggct gagtttttct gttataataa
    24601 ggaatatgaa aagcgtttac aaaggagaag ataaatgcaa agattaggac aaacaggtct
    24661 agaagtttca cgaattggtc taggttgtat gcgaatggct agtctgacag agcaggaaac
    24721 tgctaaagtg ttagatacag tcgttggaca gggaatcaac ttttttgatc atgcggatat
    24781 ttatggtggt ggtgaatcag aaatccgttt tgctcagggg gcaaaattgg cgggattgaa
    24841 acgtgaggac ctgcttctcc aatcaaaatg tggcattcgc aagggttatt ttgatttttc
    24901 taaagactac atcttggagt cagtggacgg tattctcaag cgcttggata cggaatattt
    24961 agacgttctc gccttacacc gtccggatgc tctaatggaa gcagaggaag tggcagaagc
    25021 cttttatctc ttgaaaaaag cagggaaggt tcatcatttt ggcgtcagca accaaaacat
    25081 ttaccaaatg gagttattac aatcctattt cgatcagccc ctagtagtta atcaattgca
    25141 gctctctcct gctcacacgc ctttgattga tgctggatta catgtcaata tgaaagatga
    25201 tgctgcgact atgcgtgatg gtggactgat tgattattgc cagcttaaaa aaatcactat
    25261 tcaggcttgg tctcctttct tgattgattt gcaacgaggt atttttgcca atcatccaaa
    25321 ctatgctctt ttaaatcaaa ctattggaga aatagcagag cgatacaatg tttcacatga
    25381 aacaattgtc gtggcttgga ttttgcgtca tccagcaaag attcaaacca ttgttgggtc
    25441 catgaatccg gagcgcttaa aaaagattgc acaagctagt cagattatct tgactagacc
    25501 agaatggtac gacatttata gaagtgcggg caatattttg ccgtaggagg caactatgca
    25561 gtgggaaata aaagcatttg accaacttag tttacaagaa ctttacacta tcttgacact
    25621 tcggaccgat gtgtttgtcg tggaacaagc ctgtccctat ccagaaatag atggaaaaga
    25681 tccaagttgt cttcatcttt taggaacaga caatggggag ttagtggctt atttgagaat
    25741 cttgccagct ggtctaagtt atgatgaagt ttccatcggt cgtgtggtca tcaagccaag
    25801 ccatcgtggt aaaggattgg gtcggccaat gatggagcaa gctatcgctt acattacaac
    25861 cgagtggaag gaaagtcaga ttaaaatcgg ggcccaagcc tatttggaaa aattctatca
    25921 atcgcttggc ttcgaaccta tttctgaagt atatttggag gatgatatcc cacatttgga
    25981 tatgttatac cgtaaactag tcgtttaagg ctggtttttc gatttttctc ggcaatttcc
    26041 cccaaaaatc agccatttta tggtagaatg aataggtata aaatcagtaa aagaggaaga
    26101 aaatgaaacg ttctatgtac gctggacgtg ttcgtaagga acatgtcgga caggaaatta
    26161 ctttgaaagg ttgggtcggc cgccgtcgtg acttgggtgg cctgattttc attgacttac
    26221 gtgaccgcga aggcattatg caattggtga ttaaccctga gtcagttgaa gctgaagtga
    26281 tggcaaaagc ggagagtctt cgttctgaat ttgttatcga agtaacagga acggttgtgg
    26341 agcgtgagca agctaacgac aatatcccga caggagctgt tgaactgcaa gtaaccagct
    26401 tgacagtgtt gaacacggct aagacaactc catttgaaat caaggatggc attgaagcca
    26461 gcgatgacac tcgtcttcgt taccgttatt tggatcttcg ccgtccagaa atgctcaaca
    26521 acttcaaatt acgtgcggct gtaacccaca gcattcgtaa ctacctagac gagcttgaat
    26581 ttatcgatgt ggaaacgcca atgttgacca agtcaacgcc agaaggtgcg cgtgactact
    26641 tggtaccatc tcgtgtgtcc aaaggtcatt tctatgcttt gccacaaagt ccacagatta
    26701 ctaagcagct tttaatgaac gctggttttg accgttacta ccaaatcgtt aagtgtttcc
    26761 gtgatgaaga tttgcgtggt gaccgccaac ctgagtttac acaggttgac ttggaaacct
    26821 cattcttgaa tgaggttgaa atccaagaca tcgtagaagg cttgattgct aaggtcttga
    26881 aagataccaa aggaatcgat gtgaccttgc cattccctcg tatggcctat gaccatgcta
    26941 tgaacttcta tggttcagac aagccggata ctcgttttga gatgcttttg caagacttga
    27001 cagaacttgt caaagaagtt gacttcaagg tcttctcaga agcaccagtt gtcaaagcca
    27061 ttgtggtcaa gggtgcggcg gattgctact cacgtaaaga tattgataaa ttaacagact
    27121 acgcaaaaca atttggtgct aagggacttg cttgggttaa agtagacaag ggagaattag
    27181 cgggtccagt tgctaagttc ttgacgggta ttacagaaaa cttgacagca agtttacaac
    27241 ttgaagacaa ggacttggtt ctctttgtag cagatgaatt ggaagtagcc aataataccc
    27301 tgggagcttt gcgtaaccgc ttggcaaaag agcaaggttt gattgacgaa agtaagttca
    27361 acttcctctg gattgtagac tggccaatgt ttgagtggtc tgaagaggaa ggtcgctata
    27421 tgagtgccca tcacccattc accttgccga cagaagaaac agctcatcat ttggatggtg
    27481 atttggcgca ggtgcgtgca gttgcctacg atatcgtttt gaatggttat gaattgggtg
    27541 gcggtagcct tcgtatcaac caaaaagaca tgcaagaacg gatgtttaaa gcgcttggct
    27601 tctctgctga ggatgcccat gagcagtttg gtttcttgct tgaagctatg gactacggct
    27661 tcccaccaca tggtggattg gctatcggtt tggaccgctt tgtcatgtta ttggctggtg
    27721 aggataacat tcgtgaagta attgcctttc cgaagaacaa caaggcatct gatccgatga
    27781 cacaggcacc gagcacagtt gcatcagcac aattagagga attggcttta aacattacac
    27841 ttgaaaatga ataaattttt cagaaaacag cgtgtacgct ttttaagaaa ggccagaaaa
    27901 aataagtttt ggtcggtcat tttgggaatt tctcgggaaa aatatgccga acggatatcg
    27961 gggtccatta tttatggact cctatctagt attgcagtca attttttctt tagaccaggg
    28021 aatgtttatt cgtctggagt tactggtctt gcacagattg tttctgctct ggcagcttct
    28081 tacgctggtt ggaatgtacc gattgcggtg gtctactacg gcctgaacat tcccttgctt
    28141 atcttagctt ggtataaaat tggttataaa tttactatct ttacctttat tacggtatcc
    28201 ttcagttctt tctttatcca atttatgccg ccagttaccc tgacgacgga tccgttagta
    28261 aatgctattt tcggtggtct tatgctggga actgggattg gctttgcact taggaacaat
    28321 gtatccagtg gtggtactga tattgtctct attattattc gcaagaagac gggacgtcaa
    28381 gttggctcca tttccttgat tgtcaacatt atgattatgt tgattgcagg gatgaccttt
    28441 ggctggcaat atgccctgta ttcaatggtt gcgctgttta tctccagccg catgacagat
    28501 gcgattttcg tgaaacaaaa acggatgcag gccatgatta ttaccaatca tcctgaacga
    28561 gtcattaaga aaatccataa gaagctcaat cgcggtgtga ccattatcga cggtgctgag
    28621 ggtgcttata accatgagca aaagacgatt ctcattacca tcatcactcg tgcagagttc
    28681 aatgatttca agcatattat gaaaaaggca gaccccaaag cctttgtatc ggtagctgac
    28741 aacgtcaata ttattggtcg gtttgtggaa agtgactgat tgacaaacac tacatgtagg
    28801 agtaaaatag tatttacagt ctacatgtag tgtttttatt ataaagagga aatgtatgaa
    28861 gaagaaaata tatttggttt acatcagttt gagtggcaat acagagagtt ttgtgaaacg
    28921 tctaaagtcc tttttccaat ttcaaactga ctgggagatt gagctggtcc atgttaaaga
    28981 tttggtcaag caggatattc ctttctatca gctagatgca ccctttgtcg cctttttacc
    29041 aacctactta gaaggtggaa atggagtgga caacggagat gtggaaattc tgaccaatcc
    29101 tctcggagat tttatcgctt tggggaccaa tgcagagctc tgtctgggtg tcgttggctc
    29161 aggcaaccgt aattttaaca accaatattg tctgactgct aagcaatatg ctgagcgatt
    29221 taattttcct gttatagaca cctttgaact cagagggatg cagaatgaca ttgaacgcat
    29281 tggtcggaag ataatggaat tgatgtaaag agcctcaact cagctttatg ctgggttgtt
    29341 tttttggtca cacaaaaaaa actcaccagc gtgaccgatg agttttaagg agattatgaa
    29401 aaatatttag gattgactat agtatacctc agactatatc gtgtttcatc ggtctaaagt
    29461 cctatttgtc ttctaaaatg caaatactct gtcctgcaat cgtgatttct tgctgatcac
    29521 gctcttcgac tggaggatag ttgtagagaa ctgccttatc tttttcacgt tcataggtaa
    29581 agtcgctatc cgcaaagttg atgataaatt gaatggtctt ttcttcactt gtcacttgat
    29641 agcgaagaac atattcagtt aaccagtaga aatcacaagt ttcttggata ctttcgtagt
    29701 tatcctggct gagtattgga ttattttttc ggaaggtaat taattcctga atgaactcga
    29761 tatgttcctt ataacgaata gctcgtgtcc agtccagacg gttgatacgg tcagggacat
    29821 tgtaagtatt atctatttca tcttttgtac ggaaaaattc ctgtccgctg tgaatgaagg
    29881 ccataccctg agagattaag atgagctgga gaccaaaact tgcagcacgt ttctgctgtt
    29941 gaggtgtcca gtttggattc tcaatatgga aatagtcaaa tgctgtagca ttatcatggc
    30001 attcgatgta gttgagggac tgttgaggcg ttaagaaatg gcttaatcgg ctaccagtca
    30061 ggagatgttg taccttttcg tgcaattgct tatctactag acggtttgga ttgagcaaga
    30121 gtttcttaaa ggtatcacgg tagtcgtcat tgaagaagct gatttcaggt aactgctccg
    30181 cattgtactg gtgagccagc ttttcaaatt cgaggcctgt tgccattttc catccttcac
    30241 catagaggta gacatttggg tgaatggcag ataattcaat ttgaatctgg ttgattgttt
    30301 cgctatctaa aatacccata aggtcgaaac ggaagccatc aaaaccatag agttctagcc
    30361 attggcgaag ggattgtttg atgtagttgc ggaccatact gcgctcgctg gcaacgtcgt
    30421 taccgcagaa tgtcccgtct gttcgcatgc cgtggtcatc atagcgatag aagtagcctg
    30481 gaacgatgag ctcaaatgcg tacttttcag catgatagac gtggttgtaa acaacgtcca
    30541 taataactga aatatctgca tcatggtagg cctggatagc ttcttgtagc tctagaatac
    30601 gtgcgtaagg atcgtttggt tggctagaat aggaaccttc tggtagattg tattgcatag
    30661 ggtcgtagcc ccagttgtaa acagcttggg gtttattttc atccacactg ccaaaatcat
    30721 aaacaggcaa taattgaata tgggtaacgc ctagttctct gatgtaatcc attcctagct
    30781 tcattccatc catggtagga gattctgtca atccgagaaa ttgactgcga tgcttgaacc
    30841 cagcttcttt ttgccatgag aaatcacgga cgctcatttc atagataatg gcctgagaaa
    30901 taggtcgttg ggtcttagca cgacgaattt tatgaagttt atctggattg ataacgtagc
    30961 tgtcacctga gttggctgtg gaagacaggg cgtagggatc gtgaacggaa atccattcac
    31021 tgtttacttt gtggagatag tgatagctgt gcgcttctag gtctccttca acagtaattt
    31081 cccaaactcc ctttgctccc ttcgtcattg catgtgggtt tccttctaga acaagaaaaa
    31141 cttgcttaga aataggtgcc cataacttaa aggttgttgc ctctggtcga taggttgctc
    31201 ccaagtcttg cccattgtat gtataacttt gttcaaaaag tgtagagcga acgatatgac
    31261 catagccgag ttctgtttta ttacggtctt ggtcgtagat ggtatagtcc ttgtccaatg
    31321 tcagtgcatg gaggctggtt agataataga tgacgagatt atctacttct agacaggaat
    31381 ggataaaaag ttgagatgta gcatcatttg attcaagtga gaagctcata tgttcggagt
    31441 cgaatcgttt ttccatgacc aagcgaatag ttgctacatc gtcaagagat gcttgaaatt
    31501 gtcgtaatgc catgagccaa aaggctcctt tctaagttag agttcggtaa tggtatgtgg
    31561 aatgacacgt cggtagcaga cttgcttgtc ttccttgttg tcattgatga tttggtgcag
    31621 agtgttgaat gcgacgcgtc ctagtttaat agcgtggacg tcaacgtagg cttcaatatc
    31681 tagttttggc ttgacggagt caaaagaaat gataggaagt ttgactccga cttcgttgag
    31741 atatttgaca acaccttcgg caacggaagt gtcagttgtg acgattgcat ccaggtcttg
    31801 tttgagtaat ttcttagaaa ttttgtaggc actgtcttcg agaaggaagc cagacacaaa
    31861 cttgaccttg ttttcatcca ggggaatatt gtgcgattta agggcgttct tgtaacctgt
    31921 ataacggtct tgagatacga ctaactcttt gttaccagcg acgaaggcaa tgtttttgta
    31981 acctttattg atgaagtagt tggtagcttc aaagccggcc tggatattat cattatcaac
    32041 tagtgatatg aatggagaat ccgccttacc aagaatgagg aaggggaatt tgtgttggat
    32101 agctaattgt accaatggat cgtcgggctt ggaatagagg aagatgaggc cgtccactcg
    32161 tttgccgtag accatctgag aaatcgcttc tagacgttgt tcttcattct ttcctgtgga
    32221 gatttggatg gcataattat gatctgccgc aacttgagaa attcctcgta aggcagtagg
    32281 gaagaagggg ttttgataga aaacatccga gtcatctggc aaaaccagtc cgataacctg
    32341 tgtatagcta gaaactaggc ttcgagcgtt gaggtttgga tggtagttga gatccgccat
    32401 ggctttccta accaagtctt tcgttttttg gctaatggca gagctatttt gaatgacacg
    32461 agtaactgta gatggcgaga caccagctaa tttggccaca tctttgatgg tagcacgcat
    32521 atagacctcc tagatttcat ctctgaattt tgttttgaaa aatacccata acaagcatag
    32581 cacaaatagg atattttgta tggttagaag ggtagtcaca ggttgaccaa atagtccaat
    32641 tagacaggct ataagagtag gcaggcccag acagttgaga gcaaaattat agcactctgc
    32701 aaacgtttca aaatggaaga aacgagactt tttagtcaaa aagaggaaga agctggctcc
    32761 tagtaggatg aagagtagtt ttgttgttaa aataaaggtc gaaataagag tgatggacag
    32821 tccgacagct gctcggttgg cttgatacca ttttttagag atggttgcag tcaaggtctc
    32881 cttactagca aaatcgtcag tattcaaacc ttcataagtc aattcgctta gaatatcact
    32941 tcctttacga atagtcagag actgattggc gaattgataa gtaaagtcag aactagagct
    33001 aacggtatca ccaaatgtca cttgctccca gttagtattt ccagtatagg ttaggtggtt
    33061 gttttcaatg actgcatatt gacggaggtc atctacgacc tgatctgtca gaggtgcata
    33121 gacaccttct acgaaatttt ctaaaggata ggtttcgaga ttgacagtct gtagggagct
    33181 cggaatgagc aagagcgcca ctaaaaaaag cgttgtaaac aaccgttgcc aaaggttcat
    33241 tagtctacga ttggcaaaca tatttcttgg agagaagata ctagaaaaat aggaaaaagg
    33301 atatgggagc attcgttgga cctttcataa atatcaatgt ttcccctaaa aagggaaaag
    33361 gtgggacagg gttatccctt gtcgccaccg cttgttaaac cagatacaaa gttcttttgt
    33421 aggaagaaga ataggatact gatagggagg gcaataagaa tggcacctgc cgcaaagtag
    33481 gcaatcttca tattcttcac atcactgatg aaggtttgaa gtccgacagc tacagtatag
    33541 aattcttttt cacgaagcaa gaatttggat aggatgtaat ctccaaaagg tcccatgaag
    33601 gcccagagcg cttgtacagc aatcattgga cgaacgagtg ggaggacgat ttgccagaaa
    33661 cgtctgaagt gaccggcacc gtctagtttt gcagattcat caagtgagat tggcaccgta
    33721 tcgaagtagc ctttcatgag ccaagcgttc attgggatac caccaccgac ataaaggaag
    33781 atgaggaacc agctttggtt gagcgcattg agcatgaggg ccataacgaa gaaggcggta
    33841 agagcagcca tggttggtac catttggata atcaagaaga agaccaaact ctgcttacga
    33901 gcgatgaagt tatagcgact ataagcgtaa cccgctagta ccactatact tgtttgaata
    33961 accattgtca agagagcaat gataagcgta ttgaagtacc aggttccgta tagagtctcg
    34021 ctaaagagct tgctaaaatt gtctagtgta aagttgatat tgctatccaa tttgaaggca
    34081 accacgttac cagacttgaa ggcggacatg attgtaatca agagaggata gatgatgata
    34141 acagacagaa caatcagata gagataggtt aaggtctgat tgagtatgcg tctagtcttt
    34201 acagatactt tcatcttata catcctccat ttcaaaagca tttagttttt tgaaggcaat
    34261 catggaaata ctgatgacaa tcattgagat aatcaatgta actgctgccg ccattgagta
    34321 ttgaggtgat gtacctgtgg ttaatttata aatccatgaa atcaagatgt cagttgaacc
    34381 cgctccacca ccgacgctac caggaccacc attgttgaag aggtagatga tagagaagtt
    34441 gttgaagttg aaggtgtatt ggctgattag tgttggagct gctactgcta aaatcatcgg
    34501 aagggtgatg ttacggaatt tttgaatggc acttgcacca tcaatgtaag cagcttcata
    34561 caagtcgttt ggaatggatt gcaaaatacc caaggtcaaa acatagatat aagggaaacc
    34621 aagccaagcc tgcatcataa tgagagcaac tttagtccag aatgggtcgg tcttccaagg
    34681 gataaggaaa ttgtcgatga atggtaggaa tttgctcagg aatggcaata cctgtgtgtt
    34741 aatagcgccg acactatcgt tgaacatgtt actaaaagtc aagatagtta cgaaggccgg
    34801 aacagcccat gggagcaaga agataacacc gaagatacgt tttcctttga tgaatggttg
    34861 atttgcgatg atggctgtca agataccgat aacaatttga gctgttgaag cgcagagtgc
    34921 ccagataatt gtccagccaa gtacagaacc aaatgctgta cggaatgtgc ttagtttcca
    34981 gatatttgta aagttggtaa taccaatcca atccaacaat gcgcttggtg gcaggtgttt
    35041 gaagtcgtag tttgtaaagg cgataaacaa tgtaacgaca actgggaaga tgatagcaaa
    35101 ggtcatcgca atatatgatg ggataatcaa gagatatggg aaaccattct catagatacc
    35161 ttttaccatt tcttttaatg tggtagccac tggcatccct gaattccatc gtttcgcggt
    35221 atcacgtgcg tcttttaggt tgagtgcata gaaagcgaga tagacaactg tgataattaa
    35281 gtggaaagaa ccacgaatca gcatgaatag cgaattatct tgtccacgaa cactaccgag
    35341 agtaatcaag ttgcctaatt caccagctgc aatagcaata aaataaacaa ggaaggcaag
    35401 cgtaacacct aaaaagatgt atcctttggc tttttgttta ttgtagattt gtccgagacc
    35461 agggatcagg gacaaaagga gtgccttacc tggattttgt ttcatagtca tagtctcctt
    35521 atatagcttc tgaatagacg gtggatgtta tcgtcattta gtttttcttt tattacttcg
    35581 ttaactcgcc ttgtctaact tcagttatgc ctgcggctca ttgcctcgta ctaaaagtaa
    35641 actaaaagac tatatctatt cagtttatcg ttaagtaata aagtagactg ggaagagaac
    35701 tcccagtcca actttaaact tgactagttg ctgtgttttt gagcaatttc gtctgcaata
    35761 gctttcacag catctgttgc agcagtctta gcgtcttttg aaccacttgc agcttcgaag
    35821 agcatgttac cagctggtgt ccaaactgaa cccatttctg agatgtttgg cattggttga
    35881 gctgaagcaa actggttgat aactgcagtt gtcaattcgt cttttttgct tacagcatat
    35941 tcacgagctt cagtgttagc tggaacttcg tttgtagcgt cgtaaagtgc tttttgttgg
    36001 tctgtagcag tcaagaagtc aacaaacttt tgagccattt cttggttttt agcgcctgct
    36061 ggaacaaccc atgctttacc accaccgaat gctgagtagt ttttgccgtt tacaagagtt
    36121 ggtattgttg ctacaccata gtttacacca gcttctttat atgaagctgc tttccaagga
    36181 ccttcgatga tagcagctgc tttaccgtct gtaaattgag tgttaatcaa gttgtttgca
    36241 gcagtaccgt cttgaagacc ttgaggccat ttttcgtacc aagttttagc gtattcaatt
    36301 gctttgatgg caccttcatt agcaagtccg atgtcttttg gatttgtacc attttcaccg
    36361 aatacatagc cgccgtaacc agaaagaagt ccgtatgtat agtagaaatt cgtccagtca
    36421 gctaggaatg ctgaagtttt accctcttca ccagcaaatg cgtatttgct gtctttagca
    36481 agtgtttcaa gttctgcaaa tgtcttagga gcttcagtca acaagtcttt gttgtagtaa
    36541 agaacaagtg tttcgataac cgcaggtgaa ccgtaaactt taccaccgtt tgttacaaga
    36601 gcagttgttg tgtcatctgc tttgctatca tctgcaagtg tcaattcaga tagttgacct
    36661 tcagaaccaa ggctacctac acggtcgtat ggtgccatca taacgtctgg ggctgaacct
    36721 gattggttat ccaatgataa gttatccaaa ccagttaaag catcaccagt tttaacggta
    36781 acagatacac cattttcttt ttcgaagcca gctttgacat cgttgatgta agattcataa
    36841 ccttggtcaa cgtagacagt caattctttg ctaccttcag cagacttaga tgcctctgtt
    36901 gatgaacctg agtttgagca tgcagctaaa acagcagttg atgcagcaag aagagcaacg
    36961 ctcttaagga gattgtgttt cattttcttt cctccgaaga taaatagttt tgtgtgacgc
    37021 ttgctttgcg caaacgtttt cctaagttga cttcattata cactagattg aatccgtttg
    37081 caagcgtttt tataaaaatt tttaaa
//
LOCUS       DBNFEG010000003        36540 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.003, whole genome shotgun sequence.
ACCESSION   DBNFEG010000003 DBNFEG010000000
VERSION     DBNFEG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 36540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36540
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.003"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..911)
                     /locus_tag="AC1PNG_000069"
     CDS             complement(<1..911)
                     /locus_tag="AC1PNG_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HIP6700615.1"
                     /translation="MNKRLLDYSASDFLSASPMELKQAILASEGRTVMAENVPSRSSF
                     LSGVSNAEMERAAGADLIMFNALDLFHPLIAEIPEDVQENPIVWIKKALGRAIGVNLE
                     PVDLEANMVETRAEISIGRIVSPKTLKKATELGFDFICLTGNPGTGVSNQSIFSAISM
                     AKEYFSGLIIAGKMHGAGVDEPVMNLEIAKQFIHSGIDILLVPAPYTVPNFVAEELKE
                     IVDYVRSYNKDQTIENKVLVLTANGTSQDSSDTDTIKKIALTSKACGADIQHIGDSFN
                     GIALPENIFALGQAIRGKRHQLTMLARS"
     gene            1068..1775
                     /locus_tag="AC1PNG_000070"
     CDS             1068..1775
                     /locus_tag="AC1PNG_000070"
                     /inference="COORDINATES: protein motif:HMM:NF012610.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIP6700616.1"
                     /translation="MRDNSTKSPLYYQLFDSLVEYIQKDLQPHDQLPTEKEIGTDYSV
                     SRTTVRLAMQELEKHGYIYRIQGKGSFVSSLKQENINSFFSLELKNHYDGLETADFES
                     HLVSFTKENGSLALKHLFGLSKQTNLVRIQLEHLLHSELVATETIVAKASYMPELTQE
                     SLEFEGLDSLFQKTGAITKAVDEHYSLARFMPTSSEIDALKITKSIFNQDNELMLTST
                     RIINIQKLPYRNFIWTN"
     gene            complement(1872..2189)
                     /locus_tag="AC1PNG_000071"
     CDS             complement(1872..2189)
                     /locus_tag="AC1PNG_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIP6700617.1"
                     /translation="MNIDEKDLLPIMQLIMYSGDAKGHGMRAIQVAKEGCFDEADDLL
                     RQAHDSLIQAHHVQTDMLTREAAGDTLSIGLLMIHSQDHLMNAITFLDLAREMIDLYK
                     KLG"
     gene            complement(2199..2516)
                     /locus_tag="AC1PNG_000072"
     CDS             complement(2199..2516)
                     /locus_tag="AC1PNG_000072"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIP6700618.1"
                     /translation="MKRIVLACSAGMSTSLLVTKMQQAAEAQGFEATIYAVSVSELVL
                     EVEKNPADIILLGPQVSYMEEEFKKDPHLANIPIAVINMLDYGTMNGAKVIEYAKSLL
                     DLS"
     gene            complement(2644..2877)
                     /locus_tag="AC1PNG_000073"
     CDS             complement(2644..2877)
                     /locus_tag="AC1PNG_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIP6700619.1"
                     /translation="MLTSIIAGAIIGLIAGALTSSDDRRGCLGNIILGLAGSWIGQLL
                     FGDWGPQFAGMALVPSVFGAVLLVAIFSRSRRD"
     gene            complement(2936..4177)
                     /locus_tag="AC1PNG_000074"
     CDS             complement(2936..4177)
                     /locus_tag="AC1PNG_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIP6700620.1"
                     /translation="MVLPNFKENLAKYAKLLVSTGINVQPGHTVQLTIGVEQAELARL
                     IVKEAYAHGAKEVLVNWLDDVIARERLVNVDVELLEQVHPQRITEMNYLLERKASRLV
                     VLSEDPGAYDGVDPEKLSRNTRALSQALNPMRQASQANKISWTLGAASGLEWAKKVFP
                     NAASDEEAVDLLWDQIFKTCRIYEEDPIKAWEEHEARLVAKAKVLNDEQFVKLHYTAP
                     GTDLVLGMPKNHLWEAAGSVNAQGEHFIANMPTEEVFTAPDYRVADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGEIVDVTAEKGDEVMKKLVFDNAGARGLGEVALVPDKSPISQSGV
                     TFFNTLFDENASNHLAIGQAYAFSIEGGTEMIQEELKEAGLNRSDVHVDFMIGSNKMN
                     IDGIREDGTRVPIFRDGEWAI"
     gene            complement(4674..5276)
                     /locus_tag="AC1PNG_000075"
     CDS             complement(4674..5276)
                     /locus_tag="AC1PNG_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIP6700621.1"
                     /translation="MSNFAKLQEIRRTIYALGKDLPVSNEEVVALVEKAMKESPSAFN
                     SQSSRAVVLFGAESEAFWNEIAYSELEKVTPAEAFEATKGRLAGFAAGAGTILFFEDQ
                     DVVKGLQESFPLYAENFPIWSEQAHGINLYAVWLAFAEKNIGMNVQHYNPLVDAQVAE
                     KYGIPANWKLRAQAPFGGIAAPAADKDYMADSDRVKVFGN"
     gene            complement(5511..6182)
                     /locus_tag="AC1PNG_000076"
     CDS             complement(5511..6182)
                     /locus_tag="AC1PNG_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIP6700622.1"
                     /translation="MKKRNILVELLKGIGLVLLSTILWTLILSFATPESSSGIYEPFP
                     GFTVIAGILTSFILHIIMKYNQLRQAEQAAKASKSHIVVIEERNQALMKKANRLVEKH
                     QNLEKETILDINKSVSKIRTVEEKTVMKSTKIESSQEFGQFLCQMPELMANKHIERLL
                     NEIFETENNLAQWKISYNQQVEYFNTLLHQFPISVIAKIIRLKDLEFYKEHSLTDFTD
                     EMLGL"
     gene            complement(6194..10585)
                     /locus_tag="AC1PNG_000077"
     CDS             complement(6194..10585)
                     /locus_tag="AC1PNG_000077"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002907973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HIP6700623.1"
                     /translation="MSDKFQLLLQQIGMPLDARQSGAFSTATIEKVVLHKVSKLWEFT
                     FRFETPLPLMDYQLFKARLATEFEKVGNKIQFSIVSDAEAFEAGLVEAYYPEAFTEDL
                     CQSAGFKALFQPLEVAYRDGVLWIKGPETIDTDHFRKNHLPNLVEQYKRFGFGNLAVD
                     IQVCQEMTQQQAEIFHAQNEEIYQQANEENLAALEQLAQMAPPPEAAQPFVPEYKKNR
                     PAKVNIEKAEITPMIEVDSEENRIVFEGLVFEVEQKTTKTGRVIINFKMTDYTSSFTL
                     QKWAKNEEEAQKFDMVKKGNWLRVRGNVETNNFTRDLTMNVQEVQEVKKEIRKDLMPE
                     GEKRVEFHAHTNMSTMDALPAVEDLVARAAAWGHKAVAITDHGNVQSFPHGYHAARKA
                     GIKPLFGMEANIVEDSVPIAYNEADVVLSDATYVVFDVETTGLSAVNNALIQIAASKM
                     HKGNIIAEFDEFIDPGHPLSQFTTDLTGITDEHVRGSKPLEQVLREFQDFCQDSVMVA
                     HNATFDVGFMNVNYERAGLPIISQPVIDTLEFARNLYPDFKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFLKDALEKHNLTNLNQLNTELIAEDSYKKARVKHATLYVIN
                     QVGLKNMFKLVSLSNTKYFEGVPRIPRTVLNAHREGLILGTACQEGEVFDDLLSKGID
                     EAVKTAAYYDFIEVMPPALYAPMIAKEQFKDKAEIEETIKQLIEVGRRAGLPVLATGN
                     VHYIDPEEEIYREIIVRALGQGAPINWTIGNGENAQPAPLPKAHFRTTSEMLDEFAFL
                     GESLAREIVITNPNAMLDRFEDVQVVKTDLYTPYIEKAEETVAELTYQKAFEIYGNPL
                     PDIIDLRIEKELTSILGNGFAVIYLASQMLVHRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPMPPHYVCPNCQHSEFITDGSYGSGFDLPDKDCEKCGTKYKKDGQDIPFETFLG
                     FDGDKVPDIDLNFSGDDQPSAHLDVRDIFGEENAFRAGTVGTVAAKTAYGFVRGYERD
                     YGKFYRDVEVERLAAGAAGVKRTTGQHPGGIIVFPDYMDVYDFTPVQYPADDVTASWQ
                     TTHFNFHDIDENVLKLDILGHDDPTMVRKLQDLSGIDPQTIPADDKGVMALFSGTEIL
                     GVIPEQIGIPTGMLGIPEFGTNFVRGMVEETKPTTFSELLQLSGLSHGTDVWLGNAQD
                     LIKAGIANLSTVIGCRDDIMVYLMHAGLPPKMAFNIMERVRKGLWLKISEEERNGYIQ
                     AMKDNKVPDWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPLYYYCAYFSIRAK
                     AFDLATMSGGLERVKAKMEEIALKKKNNEASNVEQDLYTTLELVNEMLERGFKFGKLD
                     LYKSHATDFLIEDNTLIPPFVAMDGLGENVAKQVVAARAEGEFLSKTELRKRGGLSGT
                     LVEKMDEMGILGKMPEDNQLSLFDDLF"
     gene            complement(10709..11698)
                     /gene="dhaQ"
                     /locus_tag="AC1PNG_000078"
     CDS             complement(10709..11698)
                     /gene="dhaQ"
                     /locus_tag="AC1PNG_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DhaKLM operon coactivator DhaQ"
                     /protein_id="HIP6700624.1"
                     /translation="MVFIRNRQATIIEDYLDGFCRTNPKVKKVKGLPIVLQKVQDDHL
                     VPIISGGGSGHEPAHIGFVGKGMLTAAVYGEIFTPPTAEEVLAAIRAVDKGKGVFLIV
                     KNFEADLVSFRKAIELARQEGIAVKYIVSHDDISVDTKNNFRMRHRGLAGTILLHKIL
                     GAAALTGYSLDELEQLGLSLATEIATIGFATKSAYLPNAALPLFDLEEGQISYGIGIH
                     GEEGYRTVRFESSEQLANEIVNKLKLKFRWKEGEDYILLVNNLGSMSEVEQGIFLNDI
                     CQFLELEGLQLSYIKTGKFMTSLDMGGISVTLCRVKDRKWLDFLEAPTEAAAW"
     gene            complement(11709..12254)
                     /gene="dhaS"
                     /locus_tag="AC1PNG_000079"
     CDS             complement(11709..12254)
                     /gene="dhaS"
                     /locus_tag="AC1PNG_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HIP6700625.1"
                     /translation="MPTSLITKKRIAKAFKKQLSQKSFDKISVVDIMQEAGIRRQTFY
                     NHFLDKYELLDWIFETELQEQVTDNLTYISGIKLLDELLYYIESNQSFYRQLFEIKGQ
                     NDFVSYLEGYFMVLMEKIINEYQLQEGKMISQQDKHFLILYHANAWIGLIKEGLTQSP
                     CQIHSYWKQMVALVRESFVIL"
     gene            12402..13391
                     /gene="dhaK"
                     /locus_tag="AC1PNG_000080"
     CDS             12402..13391
                     /gene="dhaK"
                     /locus_tag="AC1PNG_000080"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HIP6700626.1"
                     /translation="MKKIINQPEHVVDEMLKGLVFMHQDLVDRIDGFDVIIRKAEKTG
                     KVGIISGGGSGHEPSHAGFVGKGMLSAAVCGAVFTSPTPDQILEAIKAADEGAGVFMV
                     IKNYSGDIMNFEMAQELAEMEGIEVDSVVVDDDIAVENSLYTQGRRGVAGTILVHKIL
                     GHAAETGKSLAEIKELADRLVPQIKTIGLALSGATVPEVGKPGFVLEEDEFEYGVGIH
                     GEPGYKKEKLQPSAKLAEELVEKLAEGFDIQDGEHYGVLINGLGATPLMEQYVFANDV
                     AQLLAKKGVVVDYKKIGNYMTSIDMAGLSLTLIKLEDPSWIDALQAPVEVVAW"
     gene            13402..13977
                     /gene="dhaL"
                     /locus_tag="AC1PNG_000081"
     CDS             13402..13977
                     /gene="dhaL"
                     /locus_tag="AC1PNG_000081"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HIP6700627.1"
                     /translation="MDAKQALLWMETFNKKIQEDKDYLSELDSPIGDGDHGGNMARGM
                     AAVMQELSEKDYETADQVFKVVAMQLLSKVGGASGPLYGSAFMGITKASQAGADLADT
                     LQAGLEMIQKRGKAELGEKTMVDVWIPVIAALRESQLTTEIIHKAVQGTKDIAATKGR
                     ASYVGERSIGHIDPGSFSSGLLFEAMLEAGL"
     gene            13974..14348
                     /gene="dhaM"
                     /locus_tag="AC1PNG_000082"
     CDS             13974..14348
                     /gene="dhaM"
                     /locus_tag="AC1PNG_000082"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HIP6700628.1"
                     /translation="MTKVGILLVSHSKNLAQGIIDLVSEVAKDIPITYCGGLEDGSIG
                     TSFEIVQERVEANPADTLLAFFDLGSARMNMELAADFTDKQILIQTVPVVEGCYTAAA
                     LLQAGADLETILEQLKELTINK"
     gene            14358..15068
                     /locus_tag="AC1PNG_000083"
     CDS             14358..15068
                     /locus_tag="AC1PNG_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HIP6700629.1"
                     /translation="MNLIGELLGTFILVLLGNGVVASCILSKTKAENSGWLTIVLGWG
                     IAVTIAAYVSGVFGPAHLNPAVTIAMASIGSLPWAEVPGFLLAQFAGAMLASIVLYLH
                     FYPHWQETKDSGTILATFSTGPALRHTASNLLGEALGTAVLVMGILAIGPNNVSAGLG
                     PIIVGLIVFAIGFSLGSTTGYAVNPARDLGPRIMHAILPIPNKGDSDWSYSWIPVAGP
                     ILGGVAGAILYNLLLNML"
     gene            complement(15406..17268)
                     /locus_tag="AC1PNG_000084"
     CDS             complement(15406..17268)
                     /locus_tag="AC1PNG_000084"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIP6700630.1"
                     /translation="MKQSKMIIPTLREMPSDASVISHALMLRAGYVRQISAGIYSYLP
                     LANRVIEKAKNIMREEFDKIDAIEFLAPALLSADIWRESGRYETYGDDLYKLKNREGS
                     DFILGPTHEETVTLLARDAVQSYKQLPLNIYQIQSKYRDEKRPRNGLLRGREFIMKDG
                     YSFHASYESLDQTYDDYKAAYEAIFTRAGLEFKAIIGDGGAMGGKDSQEFMAITPDRT
                     DLDRWVVLDKSVASFEEIPEDVLEAIKEELLAWSVSGEDTIAYSSESGYAANLEMATS
                     EYKSSTAVVVEEELVKVATPDAKTIDEVAAFLNVAEEQTIKTMLFMADGEPVVALLVG
                     NDQVNDVKLKNHLGANFFDVAGPADAEKIFGAGFGSLGPVGLPENIKIIADRKVQDVK
                     NAVVGANEDGFHYTGANAGRDFQVTEYVDIREVKEGEPSPDGHGVLNFARGIEIGHIF
                     KLGTRYSDSMNANILDENGRSMPIIMGCYGIGVSRLLSAVLEQHARLFVNKTPKGEYR
                     YAWGINFPKELAPFDVHLIPVNVKDEAAMELTQSIEASLVGAGYEVLTDDRNERVGVK
                     FSDSDLIGLPIRVTVGKKAADGIVEVKIKGTGDTVEVHVDQLLETLQILTKENE"
     gene            complement(17552..18811)
                     /gene="rseP"
                     /locus_tag="AC1PNG_000085"
     CDS             complement(17552..18811)
                     /gene="rseP"
                     /locus_tag="AC1PNG_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIP6700631.1"
                     /translation="MKGILAFIFIFGVIVVVHEFGHFYFAKKAGILVREFAIGMGPKI
                     FAHTGKDGTLYTIRILPLGGYVRMAGWGEDKTEIKTGSPASLSLNEAGVVTRINLSGK
                     QLDSLSLPMNVTSFDFEEKLEITGLVLEESKTYKVDHDATIVEEDGTEVRIAPLDVQY
                     QNATVWGRLMTNFAGPMNNFILGILVFILLFFMQGGVANPSSNAVSITEGGALQAAGV
                     VTGDKILSVNGNTTDSYTEVATIISKAATDATTAPSFDLVVEHDGKNRHVSVTAEQVD
                     GAYRIGISPILKTGFVDKIIGGFQEAGATALRVVTALKNLIANFDVKQLGGPVAIYKV
                     SSQAAEFGLVSVLGLMAALSINLGIFNLIPIPALDGGKIVMNILEAIRRKPLKPETES
                     YITLAGVAVMVVLMIVVTWNDIIRVFF"
     gene            complement(18821..19627)
                     /locus_tag="AC1PNG_000086"
     CDS             complement(18821..19627)
                     /locus_tag="AC1PNG_000086"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIP6700632.1"
                     /translation="MTNDLQKRVIFGGVALAIFIPFLLAGGMAFQFFVGLLAIIATAE
                     LIKMHRLAPNSIEGVLAMLASLVLALPLQNYLTFLPTDGNYTAYAIVVFLLLGSTVFN
                     IGHYNYSDVVFPIASSFYVGIGFHSLILARMDGLNKVFFALCLVWATDIGAYLIGRQI
                     GRRKLLPKVSPNKTVEGFAGGILSAVVVAIIFLIVDKSLLAGYSFGMMLLLVVIFSIF
                     SQFGDLVESAIKRHFGVKDSGKIIPGHGGILDRFDGMIFVFPIMHFFGLF"
     gene            complement(19637..20392)
                     /locus_tag="AC1PNG_000087"
     CDS             complement(19637..20392)
                     /locus_tag="AC1PNG_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIP6700633.1"
                     /translation="MFKFNFKKKESIETAIEVPKHIAVIMDGNGRWAKKRMQPRIMGH
                     KAGMDALQKVTKTASDLGVKVLTVYAFSTENWSRPEKEVKFIMNLPVEFYDKYVPELH
                     ANNVKIQMIGDHSKLPVPTLNALYKAEQKTKSNTGLILNFALNYGGRDEVTRAVKAIA
                     QDVLDAKFNPGDIDEKLIADYLYTGTLSPTLRDPDLVIRTSGELRLSNFLPWQTAYSE
                     LYFTDIAWPDFDGEALKLAIKEYNRRHRRFGGV"
     gene            complement(20506..20829)
                     /gene="yajC"
                     /locus_tag="AC1PNG_000088"
     CDS             complement(20506..20829)
                     /gene="yajC"
                     /locus_tag="AC1PNG_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIP6700634.1"
                     /translation="MEGLFLPLVMVAMIGFMFYSQRKQQKQRQEALNQIKKGDEIVTI
                     GGLFGIVDEIDDKKVVLDVDGVYLTFERGAIRNRVASSGTTSAVVEEVAVETTEATPE
                     AAIEE"
     gene            complement(20939..22231)
                     /locus_tag="AC1PNG_000089"
     CDS             complement(20939..22231)
                     /locus_tag="AC1PNG_000089"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIP6700635.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGTKYKLHLIPSGIFFPEKISVIGNGVVVNPKSLVKEINYLHDSGVTTDNLRISDRA
                     HVILPYHIKLDQLQEESKGENKIGTTNKGIGPAYMDKAARVGIRIADLLDKEIFAERL
                     RTNLAEKNRLFEKMYESTPIEFDEIFEEYYAYGQEIKKYVTDTSVILNDALDQGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELHDEIGDRIREIGKEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLETLKICVAYDLDGERIDHYPASLEQLKRCKPIYEEMPGWSEDITGVRSLDELPEA
                     ARNYVRRISELVGVRISTFSVGPGREQTNILESVWSAK"
     gene            22596..24827
                     /gene="gshAB"
                     /locus_tag="AC1PNG_000090"
     CDS             22596..24827
                     /gene="gshAB"
                     /locus_tag="AC1PNG_000090"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HIP6700636.1"
                     /translation="MLQKLSPNSPILQATFGIERESLRINSNHRVAQTPHPHKLGSRS
                     FHPYIQTDYSEPQLELITPIAQSTKEARRLLGAITDVAARSMDKQEYLWPLSMPPVIS
                     EEEIQIAQLDSDYEYQYRVGLGERYGKLVQSMSGIHYNFELGKDLTQQLFELSKETDF
                     IAFKNTLYLKLAQNFLNYRWLLTYLYGASSLAEKGFLTTEVGCVRSIRNSKYGYVNSD
                     DVHISFSSLEQYVADIEQAVQSGQLSAEKEFYSSVRLRGAKTSRDYLSKGISYLEFRS
                     FDLNPYDPLAISQETLDTVHLFILSLLWLDQLTDVDNTLAKADKLNNLIALSHPHTPL
                     PNDADATPILTAMKAIVLHFGLDDYYGQLIAHVEAALQDPRLTLSGKIAEQIEDGSLE
                     KFGQQQGRLFHDYAWTAPYALKGYENMELSTQMILFDAIQLGLNVEILDEEDQFLKLW
                     HGDHVEYIKNGNMTSKDNYVIPLAMANKVVTKKILDQAGFPVPAGAEFANKTDALRYY
                     GQVASSAIVVKPKSTNFGLGISIFQEPASLADYEKALDIAFSEDSHVLVEEFVAGTEY
                     RFFVLDGKCEAVLLRVAANVVGDGSSTIRELVDQKNQDPLRGRDHRSPLEIINLGDIE
                     LLMLEQQGYTPDTVLPEGVQAFLRGNSNISTGGDSIDMTDQMDESYKQLAADMATAMG
                     AWACGVDLIIPDRTKPASKDKPNCTCIELNFNPAMYLHTYTYAGPGQSITPKILKKLF
                     PEL"
     gene            25384..28503
                     /locus_tag="AC1PNG_000091"
     CDS             25384..28503
                     /locus_tag="AC1PNG_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6359 domain-containing protein"
                     /protein_id="HIP6700637.1"
                     /translation="MKIRNRSLFYTVGSVAVTAGLLFSLATSPIPSVHATEVAIENYP
                     SLAITKSEVTIQGYLIAPLNSSGTAFDATNKTNLALGASMDTAAADTIPIQLKEPLRS
                     QFNLVDHPELVGKLVRITGTSDTYMKRAGIKPATAIEIVDSSSTNVQPPTSENTATKP
                     SDLVSTPIATVRSGAQGTEYTVSGKIISLVNGWGGNGFYLQGSDGAGIYIYPGAALGY
                     QPGDTVQLTGTLGEYKGELQLTTVSNHKAISENFNTPITETNIAQLATQAQATLVSLK
                     NLTVGEIQSDSYQNSTFTVTDSEGQTVDVRLDSRTGIKTADLLNRINKGDKINLTAIL
                     STYNGKIQLKPFDLSHFEVIEKATTEAGLGKTEAVTVGRIQGASHQSPLVNQSVMLKN
                     VVVTYVTSANNFYVQDVTPDGDIKTSDGINIFTDKLKTNVKVGDLVTIAGRVEEYQGR
                     GYAERDKTDLTITQIRATEVTVNGTAPVPSPIVLGLDRTIPADIIDNDGLAQFDPEQD
                     ALDFWESVEGMVVAVDDAKILGPLKNKEIYVTPATSQLPLNNVGGVNLRPEGNNTNII
                     PLLLKNGKQIVKSGDYFTGRIAGPVTYSYTNYKVYVDDSTLPTLHEGATKPETTTIIP
                     NDDKLTIASYNIENFSADSKSTSDAKVQRIAKSFVSDLHSPDVIGLIEVQDNNGATND
                     GTTDASKSAERLIAAIQAAGGPTYTYVDIAPENNKDGGQEGGNIRVGFLYNSKRVSLS
                     DKPIGTATQAVAWENGELNLSLGRIDPTNPAWAAVRKTLAAEFVFKGEKVVVLANHLN
                     SKRGDNGLYGKIQPVSFKSEEKRHSLAQIITDFTKAGLTQNPNANIVMLGDFNDYEFT
                     KTIEILETGGMANLVSRHDASDRFSYFYNGNNQSLDNMLVSTNLLDRYAFDMVHVNSA
                     FMEEHGRASDHDPLLVQLDVTKAQEPTQPEPSDKQTDDSGTVNNSDDNGTTNNNKPTN
                     LSTSNQTAVNADDRSAATDKGQITVTPTANNSQKKILPKTGGETSFVLITIGLVFLSA
                     CLVKKQKES"
     gene            28583..29449
                     /gene="hslO"
                     /locus_tag="AC1PNG_000092"
     CDS             28583..29449
                     /gene="hslO"
                     /locus_tag="AC1PNG_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIP6700638.1"
                     /translation="MDKIIKTLSKSGHFRAFVLDSTETVKTAQEKHDTMASSTVALGR
                     TLIANQILAANEKGDTKITLKILASGAVGAIISVANTKGQVKGYIQNPDLDYKRTATG
                     EVIVGPLVGNGQFLVITDYGTGHPYNSMTPLISGEIGEDFAYFLTDSQQTPSAVGLNV
                     LLDEEDKVKVAGGFLLQVLPGATEAEIARFEKRIQEMPAISSLLASENHIEALLSAIY
                     GDDDFKRLSEEEIGFVCDCSKDRFLDALASLPKTDLQEMKEEDKGVDITCQFCQTHYH
                     FDENDLEELING"
     gene            29442..30446
                     /gene="dusB"
                     /locus_tag="AC1PNG_000093"
     CDS             29442..30446
                     /gene="dusB"
                     /locus_tag="AC1PNG_000093"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIP6700639.1"
                     /translation="MANLNTPFMIGDVEIPNRCVLAPMAGVTNSAFRTIAKEMGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDDNEYPMSIQLFGGDAEGLKRAADFIQTNTKANIV
                     DINMGCPVNKVVKNEAGAKWLKDPDKIYHIIKEVTSVLDIPLTVKMRTGWNNTDLAVE
                     NALAAESAGVSALAMHGRTREQMYTGTVDLETLTKVAGSLTKIPFIANGDIRNVEDAR
                     QRIEEVGADAVMVGRTAMGNPYIFNQINHYLETGEVLPDLSFEDKLEVAFDHLTRLTN
                     LKGESIAVREFRGLAPHYLRGSAGAAKIRGAVARAETIEQVQELFNQAREAYQERIAK
                     "
     gene            complement(30547..31038)
                     /locus_tag="AC1PNG_000094"
     CDS             complement(30547..31038)
                     /locus_tag="AC1PNG_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HIP6700640.1"
                     /translation="MNQTIAVVFGTFAPMHKGHLDLIERAKLACGQVCVVVSGYDRDR
                     GDRIGLDLTKRFQFAQEQFKEDDFVEVRALDETDLPPYPDGWDLWLDRLLGLLDLSEH
                     QLPVFYVSEEEYAQELHSRGYQAHFSPRKFGISATLIREQPQQYQDYIAPAFLAFFGK
                     ETM"
     gene            complement(31156..33609)
                     /locus_tag="AC1PNG_000095"
     CDS             complement(31156..33609)
                     /locus_tag="AC1PNG_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIP6700641.1"
                     /translation="MKISTGLQRVFEDAQLVAQRYACDYLETWHVLLSFVINHDTVAG
                     AVLAEYPISISDYEHATFVVTDKVYREELDSFRILPSSKRLDETASFAKKIAEVVKAK
                     ELGTEHLFMAMLLDKRSTASQILDKVGFCFEDSDDKFRFLDLRKNLEARAGFTKEDLK
                     AIRSVMKGGKAKPTNMGQMMGMPPAPQSGGLEDYTRDLTALAREGKIEPVIGRDAEIA
                     RMIQILSRKTKNNPVLVGDAGVGKTALALGLAQRVAAGDVPVSLAKMRVLELDLMNVI
                     AGTRFRGDFEERMNNIINDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALA
                     RGTLRTVGATTQTEYQKHIEKDAALVRRFAKVTIEEPTVEDSIAILTGLKGTFEKYHR
                     VRIGQAAVETAVTYAKRYLTSKNLPDSAIDLLDEASATVQNRVKGQGEETGLTSIDKA
                     LMDKKYKTVSKLLIETKEDAEASQNYDLEVTEEDVLETLSRLSGIPVAKLSQSDTKKY
                     LNLEAELHKRVIGQEEAISAVSRAIRRNQSGIRTGRRPIGSFMFLGPTGVGKTELAKA
                     LAEVLFDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLL
                     FDEVEKAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGF
                     GALDLSKDHKEVEKRIFEELKKAYRPEFINRIDEKVVFHSLTETHMQDVVKVMIKPLL
                     AVTAEKGITLKLQPSALKLLAKQGYDPEMGARPLRRLLQTKLEDPLAEMLLRGDLTTS
                     STLKVGVKGEELKFDVVKG"
     gene            complement(33613..34065)
                     /locus_tag="AC1PNG_000096"
     CDS             complement(33613..34065)
                     /locus_tag="AC1PNG_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HIP6700642.1"
                     /translation="MKNTSDYIEEHIKALLEQVSVAELKRSELASRFEVVPSQINYVI
                     KTRFTASRGYIVESKRGGGGYIRIGRITFSDKHELVHDLLNNMGEQLSATVYADILQL
                     LFDEGLMTEREGNLFLFTGSDEILGKDASTIRARMMRQILRRLDRKEE"
     gene            34233..34592
                     /locus_tag="AC1PNG_000097"
     CDS             34233..34592
                     /locus_tag="AC1PNG_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HIP6700643.1"
                     /translation="MFDTNHFFEDIKDFKAVSPKEASELLAAKDGAILFLGRDTCPYC
                     RRFAPKLAAVAAAQNWTVHFLHTQDPAYSAQEIAALREQYHVPTVSGLLVAKASGVDV
                     RCDSSLTEDEILAFVMA"
     gene            complement(34664..>36540)
                     /locus_tag="AC1PNG_000098"
     CDS             complement(34664..>36540)
                     /locus_tag="AC1PNG_000098"
                     /inference="COORDINATES: protein motif:HMM:NF024296.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIP6700644.1"
                     /translation="FVMTKMKKILAFILLPVMILFSGYLGASSHVYGDETTNVITKMY
                     FTDKDGNELTPPNVEQWQQFRLNADFDIKNNAVKEGDVIEIELPSELIFNQSSDFQIT
                     DKEGNVVANATITPEPEKKIKLTYTKYVEEHSDIKGSFYFYVRVDHRVIQQKKEIPLD
                     ITVQGKIIHAGKIQFQGIGTPDKRDIQKSGWSDSKNPQDAWFIIAINRSGQSMKDVKV
                     VDVLKSKQLEYIPDTLRVEKGNWSFKAGDWVLDNIETITDQVTLTFAPDGKSFSIELP
                     DLAPTDGIQIIYKTKAPYVPVDGEEFSNEATLITTNGIDQTVTSGYTYLAAGGKAEGY
                     VFTIKVKKTDEAGAPLADVEFDVIRNRTGQSVGKITTDDQGEGSISNLLRDEYTLKEI
                     KTPDGYLPIEDMVINPAEFDGVTKSVLKEIVNKKKPAGPVKVVLKATKTLEGRALADQ
                     EFEFTLLKKDDGSVVSTAKNDGTGVVTFDELTFDAPGTYKYTVKEVLGTDAGIDYDEV
                     EYDVIVTVTDNGGPTLVATVDKSEQEVNFINKVITTTTTTTTTTTTSEETTTTPETPA
                     STTTVETTVPEVPASTTTTGKILPKTGEKSGLWTSVLGVIFLVVGAGAVIFYRQSKEA
                     "
BASE COUNT        10508 a         8781 c         7144 g        10107 t
ORIGIN      
        1 gaacgagcca acattgttaa ctggtgacgc ttccctctaa tcgcttgacc taaagcaaaa
       61 atattttcag gaagtgctat tccattaaaa gaatccccaa tatgttgaat atctgcacca
      121 caagctttag aggtcaatgc aattttctta attgtatcgg tatccgacga gtcctgacta
      181 gtaccattag ctgtcaaaac taaaacttta ttctctatag tctggtcctt attatatgac
      241 ctaacatagt ctactatttc ctttaactcc tccgcaacaa aattcggtac tgtatagggc
      301 gctggtacta gcagaatatc aattcccgaa tgtataaact gcttagcaat ctctaaattc
      361 atcacaggct catcaactcc agctccatgc attttccctg cgataatcaa accagaaaaa
      421 tattcttttg ccatagatat tgcagaaaat atggactggt tactgacccc tgtacctggg
      481 tttcctgtca agcaaataaa gtcgaagcca agttctgtag ctttttttaa tgttttcggc
      541 gaaacaattc tacctattga aatttcagca cgtgtttcaa ccatatttgc ttccaaatca
      601 acaggttcaa gattgacacc tatcgcccgc ccgagagctt tttttatcca aacaattgga
      661 ttttcttgaa catcctcagg gatttccgct ataagcggat gaaacaagtc caatgcattg
      721 aacattatca aatctgctcc tgctgctctt tccatttctg cattagaaac accactcaga
      781 aatgacgatc tgctcggaac attttcagcc atcactgttc gtccctctga cgccagtatt
      841 gcttgtttca gttccatagg ggaggccgac aaaaaatcag atgctgaata atcaagtaac
      901 cttttattca taatttatcc tttcaacttg ttataacaac gtatcgattc atcaacatta
      961 tatatccatt tgtaagcgtt gtcaactgtt tttgttgact tttttcaaaa aaaatctata
     1021 atataaccaa agatgttata ataatatcaa agaaaggatt tccgacgatg agagacaatt
     1081 caacaaaatc accactatat tatcagctat tcgatagttt ggtggaatat attcaaaaag
     1141 atttacaacc acatgatcaa ttacctacag aaaaagaaat tggaactgat tattccgtca
     1201 gcagaacaac cgtacgattg gccatgcaag agttagagaa acatggatac atttatcgaa
     1261 ttcaaggaaa ggggtcgttt gtatctagtc ttaaacaaga aaatatcaac tcattttttt
     1321 cacttgaatt aaaaaatcac tatgatgggt tagaaaccgc agactttgaa tcccacttag
     1381 tcagtttcac aaaagaaaac ggttctctag cattaaaaca tctctttggt ctgtccaaac
     1441 aaacaaatct cgtccgcata caattagaac acctgcttca ttcagaactt gttgctactg
     1501 aaacaattgt agcaaaagct agctatatgc ctgaactaac gcaggaaagt ttggaatttg
     1561 aaggattgga ctcgttgttc caaaaaactg gtgctatcac taaagctgtt gatgaacact
     1621 attctcttgc tagatttatg ccaactagtt cagaaattga tgcattaaaa attacaaaat
     1681 ctatttttaa ccaagataac gaattgatgt taacttccac tagaattatc aatatccaaa
     1741 aactaccgta ccgaaatttt atttggacaa actgaaatta atagatagaa aaaccgcaga
     1801 ttgcttgaat ctacggcttt ttatttaata ttctcttttt taaaccgaca actaattgaa
     1861 acaattctca ttcaccccaa ttttttatat aaatcaatca tctctcgtgc caaatctaaa
     1921 aaagtaattg cattcattaa atggtcttga ctatgaatca ttagtaaacc aatggaaagg
     1981 gtgtcacccg ctgcttctct tgtcaacata tccgtctgga catgatgtgc ttgaattaac
     2041 gaatcgtgag cttgtctcaa taaatcatct gcttcatcaa agcaaccttc cttagctact
     2101 tgaatagctc tcatcccatg acccttagca tctccactat acatgatgag ttgcataata
     2161 ggcaataagt ctttctcgtc tatattcatc actatctcct atgataaatc tagtagagat
     2221 ttggcatatt caattacctt agccccgttc atcgtaccat aatcaagcat attgataact
     2281 gcgataggaa tattagctaa atgtggatct ttcttaaact cctcctccat ataactaact
     2341 tgtggtccca acaaaataat atctgctgga ttcttttcaa cttctaatac cagctcactc
     2401 accgaaacag catagattgt agcttcaaac ccttgagcct cagcagcttg ttgcatctta
     2461 gtcaccaata agctagtact cataccagca gagcacgcaa gtacaattcg tttcattttt
     2521 gattctccta taaataatta tctattgata tggcgatagg atcgtacgtt gttataacaa
     2581 ctataacatt tttaaaaact ttttgcaagc gctctcttag acaatttttc aatcagtgca
     2641 gttctaatcc ctccgactgc ggctaaaaat agccaccagc aatactgctc caaagactga
     2701 cggtaccagg gccataccag caaactgtgg gccccaatca ccaaagagca actgtccaat
     2761 ccatgaacct gcaagaccaa ggataatatt tcctagacag ccacgacggt catctgatga
     2821 ggtcaaggct ccggcaatca agccaataat agctccagcg ataatgcttg ttaacattct
     2881 gtattcctcc ttatttacaa aataagactg gttgcccagt cttataagat gactattaga
     2941 tagcccactc accgtcgcgg aagataggga cgcgagttcc atcttcacga atgccatcaa
     3001 tgttcatttt gtttgaacca atcatgaagt caacgtggac atcagaacgg ttgagacctg
     3061 cttctttcaa ctcttcttgg atcatttctg taccaccttc aattgagaag gcataggctt
     3121 gcccgattgc caagtggttt gaggcatttt catcaaagag agtgttgaag aaagttacac
     3181 ctgattgaga gattgggctc ttatctggta caagggcaac ttcacctaga ccgcgtgcac
     3241 cagcattgtc aaagaccagt ttcttcatga cttcgtcacc tttttcagca gtcacatcca
     3301 cgatttcacc atctttgaag gttactttga tgccttcgat gatgttacca ttgtaactga
     3361 gtggttttgt tgacgttaca tagccgtctg ccacacggta gtcaggagct gtgaagacct
     3421 cttctgttgg catgttggca ataaagtgtt caccttgggc attaaccgaa ccagccgctt
     3481 cccacaagtg attcttcggc atacctagaa cgaggtcagt tcctggtgct gtgtagtgca
     3541 atttgacaaa ctgctcgtcg ttaagcacct tagccttagc caccaagcgt gcttcgtgct
     3601 cttcccaagc cttgattgga tcttcctcgt agatacggca agtcttgaag atttggtccc
     3661 aaaggaggtc cactgcctct tcgtcagacg ctgcatttgg gaagactttt ttagcccatt
     3721 ccaaaccaga ggctgcaccg agtgtccagc tgattttgtt agcttgggat gcttgccgca
     3781 ttgggttcaa agcctggctg agtgcacgag tgttgcgaga caatttctct ggatcaacgc
     3841 catcgtaagc acctggatct tctgacaaaa caaccaaacg gctagcctta cgctccaaga
     3901 ggtagttcat ttcagtgatg cgttgtggat gaacttgttc caagagttcc acatcaacat
     3961 tgaccaaacg ttcacgcgca atcacatcgt ccaaccagtt gaccaagact tccttagcac
     4021 cgtgagcgta agcttctttg acaatcaagc gtgccaactc agcctgctcc acaccaattg
     4081 tcaattgaac cgtgtgacca ggttggacat tgatacctgt tgaaaccaag agtttagcat
     4141 atttagcgag attttcttta aaatttggta ataccatctt tttctccttt gtactttgaa
     4201 ataccctatt attgtatcat attttaagct agacagataa aaaaacttcc tgctgataca
     4261 ggaagttgaa aaggttagct ctgggagatc gttgaagctt ggagatgaaa cgaatggagt
     4321 tcgtgtcagc aagccgcagg tataactcaa caccctctta tttctaggca ttgacttgaa
     4381 atagtccccc agattgctta cacagttaat ggactgtgca aggtgggaga taaaacttgc
     4441 ggagcaagtc gcttttgcag aatcaggcaa ggcgagttca aacaatccag tggattgttt
     4501 gaagttggaa ataaggaaac gaagtttcct caactgccga cgtaataaag caaactgaaa
     4561 taaattatat atactcaatg aaaattggaa tcagactagg cgacgtagat gtagatagaa
     4621 cttaagttca tcacatcaag tcaacaacgt ctgacttcaa ttttcgcaga gtattagtta
     4681 ccaaaaacct ttacacggtc actatccgcc atatagtctt tatccgctgc tggagctgca
     4741 atgccaccga atggtgcttg ggcacggagt ttccagttag ctgggatacc gtatttttca
     4801 gcaacttgtg catcaaccaa tgggttgtag tgttgtacgt tcataccgat atttttctca
     4861 gcaaaggcca accatactgc gtacaagttg ataccatgag cttgttcaga ccagattggg
     4921 aagttttcag cgtaaagtgg gaagctttct tgtaagcctt tcaccacgtc ttggtcttcg
     4981 aagaacaaga tagtaccggc accagccgca aaaccagcta agcgaccttt tgtagcttca
     5041 aaagcttctg caggcgttac tttttccaat tcgctgtagg caatttcgtt ccagaaagct
     5101 tctgactccg caccaaaaag taccactgca cggctagatt gtgagttgaa agctgatgga
     5161 gattctttca tggctttctc aacaagtgct actacttctt catttgatac tggcaagtct
     5221 ttaccaagtg cgtagattgt acggcgaatt tcttgaagtt ttgcaaagtt tgacatatat
     5281 tttcctcttt tcttattgtt actacatagt agtttttatc actataggga gattgtatca
     5341 ttattactaa gtagtgtcaa attcctagtc taactttttt atactccgtg aaaattaagc
     5401 tagttacagc agatagtctg attttcataa agtagtgaca gagaatttac aaaaagatgg
     5461 caaaaaagcc tatcaaatac ttaagataca ttgatagacc cttctttatc ttatagcccc
     5521 aacatctcgt ctgtaaaatc agtcagcgaa tgttccttat aaaattccaa gtccttcagg
     5581 cgaatgattt tggcaatgac gctaatcgga aactgatgca acagagtatt gaaatactca
     5641 acctgctgat tgtatgaaat cttccattgt gctaaattat tttctgtttc aaaaatttca
     5701 ttcagtaacc tttcaatatg tttattggcc atcaattccg gcatttgaca caaaaattga
     5761 ccaaattctt gtgaactttc aatcttggtg gatttcataa cagttttttc ttcaaccgtt
     5821 cgaattttgg aaacggattt attgatgtcc aaaatggttt ctttttcaag attctggtgt
     5881 ttttcaacca agcggtttgc tttcttcatc aaggcctgat tgcgctcttc aataacaacg
     5941 atatgacttt tactagcttt cgcggcttgt tcagcctgac gaagctggtt atacttcata
     6001 atgatatgga gaatgaaact cgttagtata ccagcaatca cagtaaaacc tggaaaaggt
     6061 tcgtaaatac ctgatgatga ttccggtgtg gcaaaagata agatcaatgt ccacaagata
     6121 gtagaaagca gtactagacc aattcctttt aataactcaa ctaaaatatt tctttttttc
     6181 atcatttcct tttttaaaac aaatcatcaa agagactcaa ctggttatcc tctggcattt
     6241 taccgaggat gcccatttca tccatttttt caacaagcgt acccgacaat ccaccacgtt
     6301 tacggagttc tgtcttggag agaaattctc cttcggcacg cgccgccaca acctgcttgg
     6361 caacgttctc cccaaggcca tccatggcaa caaatggtgg gatgagggta ttatcttcaa
     6421 tcaagaaatc ggttgcatgg gacttgtaaa ggtccaattt cccgaacttg aagccacgtt
     6481 ccagcatttc atttaccaat tcaagtgtgg tatagagatc ctgctcaacg ttcgaagctt
     6541 cattgttttt cttcttgagg gcgatttctt ccatcttagc cttgactcgt tccaagccac
     6601 cggacatggt tgccaaatca aatgccttgg cccgaataga gaaataagcg caatagtagt
     6661 aaagtgggtg atgaaccttg aaataggcaa cccgcaaggc catcataacg taggcagccg
     6721 cgtgggcttt agggaacata tacttgattt ttccacagga ttcgatgtac cagtctggca
     6781 ccttgttatc cttcatggct tgaatgtagc catttcgctc ttcctcagaa atcttgagcc
     6841 acaagccctt ccgcacccgc tccatgatat taaaggccat cttaggtggc aaacctgcgt
     6901 gcatgaggta aaccatgatg tcatcccgac aaccgataac agtagataga ttagcaattc
     6961 ctgccttaat caagtcctgg gcattgccca accaaacgtc tgtaccgtgc gatagaccag
     7021 ataactgcaa gagttccgaa aaagtggtcg gtttggtttc ctccaccatc ccccgtacaa
     7081 agttggttcc aaattcagga atgcccagca tacctgtcgg tatcccaatc tgctctggga
     7141 taacacccag aatttccgta ccagaaaaaa gagccataac ccccttatca tcagcaggaa
     7201 tagtttgcgg atcgatgcct gacaagtcct gcaacttccg aaccatggtt ggatcatcgt
     7261 gccccagaat atcgagtttc aagacgtttt catcaatatc gtggaagtta aagtgggtgg
     7321 tctgccagct ggctgtcaca tcatcggcag gatactgaac aggggtaaag tcatagacat
     7381 ccatgtagtc agggaatacg ataattcctc ctgggtgctg accagtcgtc cgtttgacac
     7441 cagcagcacc agcagccagg cgttctactt ccacatctct atagaatttg ccataatccc
     7501 gctcatagcc tcggacaaaa ccataggccg tcttggccgc caccgtacca accgttcctg
     7561 cacggaaggc attttcttca ccgaaaatat cccgaacatc caaatgggca gacggttggt
     7621 catcccctga gaagttcaag tcaatatcgg gaaccttgtc tccatcgaaa ccaaggaagg
     7681 tctcgaaagg aatgtcctgt ccatcttttt tatacttggt cccacacttt tcacaatcct
     7741 tgtctggcag gtcaaaacct gaaccatagg aaccatctgt gataaattca ctgtgttgac
     7801 aatttggaca gacatagtga ggaggcatag ggtttacttc ggtaatccca atcatggtcg
     7861 caacgaaact ggagccgaca gacccccgtg aacccaccag gtagccccgc tcgttggaac
     7921 ggtgcaccaa catctgcgat gccaggtaaa tcacggcgaa tccattaccc agaatggacg
     7981 tcaattcctt ctcaatccgc aggtcgataa tatctggaag aggattgcca taaatttcaa
     8041 aggctttctg ataggtcaat tccgcaaccg tttcctcggc cttctcaata taaggcgtat
     8101 aaaggtcggt cttaaccacc tgaacatctt caaaccggtc cagcatggca ttgggattgg
     8161 taatgacaat ctcacgagcc agactttctc ccaagaatgc aaactcgtcc aacatctcgc
     8221 tggtcgttct aaagtgggct tttggcagtg gtgctggctg agcgttctca ccatttccga
     8281 tggtccagtt aatcggtgcc ccttgaccta gagcacgaac aatgatttcc cgatagattt
     8341 cctcttcggg gtcaatgtag tgaacatttc ctgtcgccag aacaggcaga cctgccctac
     8401 gtcctacctc aatcaactgc ttaatagtct cttcaatctc tgccttgtcc ttaaactgct
     8461 ccttggcaat catgggagca tagagggccg gtggcatgac ttcgataaaa tcataatagg
     8521 ctgccgtttt aaccgcttca tctatccctt tggagagcaa gtcatcgaag acctcaccct
     8581 cttgacaggc tgttcccaat attaaacctt cacgatgggc attgagaacg gtccgtggaa
     8641 tacgtggaac tccttcaaaa tacttggtat tggaaagaga gaccagtttg aacatatttt
     8701 tcaaaccaac ctgattaatc acatagagcg tggcatgctt gacacgagcc ttcttatagg
     8761 aatcctccgc aatcagctcc gtatttagct ggttgagatt ggtcaaattg tgcttctcca
     8821 aggcatcttt taggaagatg aagagcaggc gccctgtcgc ctccgcatcg tagttagcca
     8881 tgtggtggtg ttcaagagca acaccaaacc gcttggtgag aggacctaaa ccatgacgct
     8941 taaagtcggg ataaaggtta cgagcaaatt ccaaggtatc aatgacaggc tggctaataa
     9001 taggtaagcc tgctcgttca tagttgacat tcataaagcc aacgtcaaag gtcgcgttgt
     9061 gggcaaccat gacggaatct tgacagaaat cttgaaactc acgcagaact tgttctagag
     9121 gtttagaacc acgcacatgc tcatctgtga ttcctgtcag atctgttgta aattgactga
     9181 gcggatgacc cggatcgata aactcatcaa attctgcaat gatattgccc ttgtgcatct
     9241 tagacgccgc aatctgaatc agggcattgt taactgcaga gagacctgtt gtttccacgt
     9301 caaagaccac ataggtggca tcagacagga ctacatctgc ttcgttgtaa gcaataggca
     9361 cagagtcctc gacaatatta gcctccatcc cgaagagcgg cttaatccca gctttacggg
     9421 cagcatgata gccatggggg aaggattgaa cattgccgtg gtcggtaatc gccaccgcct
     9481 tgtgtcccca ggctgctgca cgcgccacca agtcctcaac ggcaggcagg gcatccatag
     9541 tagacatgtt ggtatgggca tgaaactcaa cccgcttctc accctcaggc ataaggtcct
     9601 tacgaatttc cttcttgacc tcctggactt cctgtacgtt catagtcaaa tcacgagtga
     9661 aattattggt ctccacattg cctcgcaccc gcaaccagtt gccctttttg accatatcaa
     9721 acttctgggc ctcttcctca ttcttagccc acttctgcaa ggtaaaggac gaagtatagt
     9781 cggtcatctt aaagttgata atgactcgcc ccgtcttggt cgtcttctgc tcgacctcaa
     9841 agaccagtcc ctcaaagaca atccgattct cctcgctatc cacctcaatc ataggcgtaa
     9901 tctcagcctt ttcgatattg accttggcag gacgattttt cttatattct gggacaaatg
     9961 gctgagctgc ctctggtggt ggcgccattt gagccagttg ttcaagggcc gccaagtttt
    10021 cttcattagc ctgctggtaa atttcttcat tttgcgcatg gaaaatctcc gcttgttgct
    10081 gcgtcatttc ctgacagact tggatgtcca ccgcaagatt accaaaacca aatcgcttgt
    10141 attgctccac aagatttggc agatgattct tccgaaaatg atctgtgtca atggtttcag
    10201 gaccttttat ccacaaaaca ccatctctat aagccacctc taatggctga aaaagagcct
    10261 tgaagcctgc actctggcac aaatcttccg taaaagcttc tggataatat gcttctacca
    10321 aaccagcttc aaaagcctcc gcatcgctga caatagaaaa ttgaatctta ttgcccacct
    10381 tttcaaactc cgtcgccaaa cgagccttga agagttgata gtccatgagc ggcaaggggg
    10441 tttcaaaacg gaaggtaaat tcccaaagtt tactgacctt gtgcaagact actttctcaa
    10501 tcgttgcagt cgaaaaagcc cctgattgtc gtgcatcaag aggcattccg atttgttgta
    10561 gcaagagttg aaacttatct gacatactta tttcctttgt agttcgttag aaacatttta
    10621 ccataaatga ggtatttttt cctgagtcag gaagaaaaaa ctctcaggtc atttcctgag
    10681 agatctgatt tctatagaaa ttagtagcct accaagccgc agcttctgta ggcgcctcta
    10741 aaaaatccag ccattttcga tccttaacac gacagagggt gacagaaatt cctcccatat
    10801 caagactggt cataaattta cctgttttta tataggaaag ctgtaaacct tctagctcta
    10861 gaaattggca aatgtcattc aaaaagattc cttgctcaac ctcagacata gatccaagat
    10921 tattgaccag aagaatatag tcttccccct ccttccagcg gaattttaat tttaatttgt
    10981 tcacaatttc gttggctaac tgctcagagg attcaaaacg aacggtccga tagccttctt
    11041 ctccatggat accgatgccg taagatattt gtccctcctc caaatcaaac aaaggcaggg
    11101 cagcattggg caaataggcc gacttggttg caaaaccgat ggtcgcaatc tctgttgcca
    11161 gagacagtcc cagctgttcc aattcatcta ggctatagcc agtaagggca gcagctccta
    11221 atatcttatg aagtagaatg gtccctgcaa gccccctatg ccgcatacgg aaattattct
    11281 tagtatcgac cgaaatatcg tcatgggata cgatgtactt gactgcaatt ccctcctgcc
    11341 gagccaattc gatggctttt ctaaaagaga caaggtctgc ctcaaagttt tttacaatta
    11401 gaaaaactcc cttaccctta tctactgcac gaatggctgc caatacttct tcagcggttg
    11461 ggggcgtaaa tatttcacca tatactgctg cagtcagcat accctttccg acaaatccaa
    11521 tatgtgccgg ttcatgcccc gatccaccac ctgaaataat tggaactaaa tggtcatcct
    11581 gcactttctg aagcacaatc ggaagtccct tcactttctt cactttagga ttggttcgac
    11641 agaaaccatc caaataatct tcaataattg ttgcctgtct atttctaata aacaccatat
    11701 attaactcct acaaaatcac aaacgactcg cgaacaagag ccaccatttg cttccaataa
    11761 ctgtgaatct gacaaggtga ctgtgtcagt ccttccttta tcagtccaat ccaagcatta
    11821 gcatgataga gaatcaaaaa atgcttatcc tgttgggaaa tcattttacc ctcctggagc
    11881 tggtactcgt tgataatttt ctccatcaaa accataaagt agccttctaa atagctgaca
    11941 aaatcatttt gccccttaat ctcaaagagt tgcctataga aagattggtt actctcgatg
    12001 tagtacaaaa gctcatccag aagcttgatt ccactaatat aggtcagatt gtcagttacc
    12061 tgctcctgca attctgtctc aaatatccag tccagcaact catacttgtc taaaaaatgg
    12121 ttgtaaaagg tttgacgacg aatccctgct tcctgcatga tgtcgacaac tgagatttta
    12181 tcaaaggatt tttgggatag ctgtttttta aaagcctttg caatcctttt ctttgtaatc
    12241 aacgaggttg gcatatgtca cttcctttcg cttcctcttt attataccct atcatgcttt
    12301 tctttggaac aaaagggaca cattggacaa tctgtccctt ccttttttct tttggacaat
    12361 gtagaatgaa agcgcaaaca aaaaaacaaa ggagttttct catgaaaaaa attatcaatc
    12421 aacctgaaca tgtcgttgat gaaatgttaa aaggacttgt ctttatgcac caagatcttg
    12481 tcgatcgtat cgatggattt gatgtgatta tccgcaaggc tgaaaaaact ggtaaagtcg
    12541 gcatcatctc aggaggtggc tctggtcacg aaccttctca tgcaggcttt gtcggcaagg
    12601 gcatgttgtc cgccgctgta tgtggcgcag tatttacctc accaacacca gaccaaatct
    12661 tggaagccat caaggctgct gacgagggag ctggcgtctt tatggtcatc aaaaactact
    12721 ctggcgacat catgaacttt gagatggcgc aagaattggc ggaaatggag ggcattgaag
    12781 tggacagtgt cgttgtggac gatgacatcg ctgtggaaaa cagcctctat acccaaggac
    12841 gtcgtggtgt agcaggtacc atccttgtcc acaagattct cggtcacgct gcagaaacgg
    12901 gcaaatcgct ggctgagatt aaggaactgg ctgaccgtct cgtcccacaa atcaagacta
    12961 tcggcttagc cctatcaggt gctacggtac cagaagtggg caaaccaggc ttcgtccttg
    13021 aagaggatga atttgaatac ggtgtcggta tccatggtga acctggctat aagaaagaaa
    13081 aactccaacc atctgctaaa ttggctgaag aattggttga gaaattggca gaaggctttg
    13141 atattcaaga tggcgagcac tatggtgtcc ttatcaacgg tctcggcgca acccctctca
    13201 tggaacaata tgtctttgcc aacgacgttg ctcaattgct ggctaaaaaa ggtgtggtcg
    13261 ttgactacaa gaaaatcggt aactacatga catcgattga tatggctgga ctgtcattga
    13321 ccttaatcaa actagaagac ccaagctgga ttgacgcttt gcaggcccct gttgaagtgg
    13381 tagcttggta ggagaaagac tatggatgca aaacaagcac tgctctggat ggagacattc
    13441 aacaaaaaaa ttcaagaaga caaagattat ttgagtgagc tagattctcc aattggagac
    13501 ggagatcacg ggggcaacat ggcgcgagga atggctgccg tcatgcagga gctatctgag
    13561 aaagactatg agactgctga ccaagtcttt aaggtggttg ccatgcaact tctcagcaag
    13621 gttggcggtg cttccggtcc tctctacggc tctgccttta tgggtattac taaagccagt
    13681 caagctggcg ctgatttggc agatacgctt caagcaggtt tggagatgat ccaaaaacgc
    13741 ggcaaggctg aattgggaga gaaaaccatg gtagacgtgt ggattcccgt cattgctgcc
    13801 ctaagagaaa gccagctgac aacggaaatt atccataaag ccgttcaagg tacaaaggat
    13861 attgctgcga ccaagggacg cgcgtcctat gttggggaaa ggtctattgg acacattgat
    13921 ccaggctctt tctcatctgg acttctcttt gaagccatgt tggaggcagg actatgacaa
    13981 aggttggtat cttactcgta tcccattcaa aaaatttggc ccagggaatc atcgacctag
    14041 tatctgaagt cgcaaaagat attcccatca cctactgtgg cggactagaa gatggtagca
    14101 tcggtaccag ctttgaaatc gtacaagagc gagttgaagc aaaccctgct gataccctcc
    14161 ttgccttttt cgacctaggt agcgcccgaa tgaacatgga acttgctgcc gattttacag
    14221 acaaacaaat tcttatccaa accgtaccag tcgtcgaagg ttgctatact gccgcagctc
    14281 tcttacaggc tggagctgac ttggagacta ttcttgaaca attaaaagaa ctgacaatca
    14341 ataaatagga ggtcattatg aatctgattg gagaattact tggaactttc atactagtat
    14401 tacttggaaa cggtgtggtt gcatcctgca tcctctctaa aaccaaggct gaaaatagcg
    14461 gctggctgac catcgtacta ggatggggca tcgccgtaac aatcgctgcc tacgtatcag
    14521 gagtttttgg acctgcccac ctgaatccag ctgtcactat cgcaatggca tctatcggct
    14581 ccctaccttg ggctgaagtc ccaggcttcc tactcgctca atttgcagga gcaatgttgg
    14641 catccatcgt cctctatctc catttctacc cacattggca ggaaacaaaa gatagcggaa
    14701 caatccttgc taccttctct actggaccag ctcttcgtca cacagcttca aacttgctcg
    14761 gcgaagctct tggtacagct gtcttagtca tgggaatcct tgcaattggt cctaacaatg
    14821 tcagcgctgg cctcggccca atcatcgtgg gacttatcgt atttgcaatc ggcttctcac
    14881 taggatcaac gacaggctac gcagtcaatc cagctcgtga cttaggccct cgtatcatgc
    14941 acgctatcct tccaattcct aacaagggag attctgactg gtcttatagc tggatcccag
    15001 ttgctggccc aattcttgga ggtgtcgcag gagcaatcct ctataacctc ctactaaata
    15061 tgttataaaa aaataggctt ttggaaaaag accagcgcaa cttatcagag ttcgtatcaa
    15121 catctcagcg cagtggttga ttggcagatt tattcgtgtt tcacactcca aatctgacct
    15181 atacgactga agcgaacaga gttcgcttca tctccaacct caaactatct cccagacagt
    15241 ttgagctgtg cgggggtggg agtgaaacag tctggggata gactgtttca gctcaacaac
    15301 ttaaaacgaa aaattgttga cgaactctgt ttgacctagt cgagttcttt cccactccca
    15361 ttttatcaac aaagacttgg ctatcatcaa ccaagtcttt ttctgttatt cattttcttt
    15421 tgtaaggatt tgcaaggttt caaggagttg gtccacatgg acttctacag tatcacctgt
    15481 gcccttgatt ttcacttcga cgatgccatc agctgctttc ttaccaacag tcacacggat
    15541 tggcaagcca atcaagtcac tgtcagaaaa cttaacgccg acacgctcat tacggtcgtc
    15601 agttaatact tcatagcctg caccaaccaa acttgcttcg atagactgag tcaattccat
    15661 cgccgcttca tctttgacat taactggaat caaatgcaca tcaaacggtg ccaattcttt
    15721 agggaagttg atgccccaag cgtaacggta ttcgcctttt ggtgtcttgt taacaaagag
    15781 gcgagcgtgt tgttcaagaa cggctgacaa gagacggcta acaccgattc cgtagcatcc
    15841 catgataatc ggcatagaac gaccattctc atccaagatg ttggcattca tactgtctga
    15901 atagcgtgta ccgagtttga agatgtgacc aatctcgata ccacgggcaa agttgagaac
    15961 cccgtgtcca tctggagaag gctcgccttc cttgacttcg cgaatatcca catactcagt
    16021 cacttggaaa tcacgccctg cattggcacc tgtgtagtgg aaaccgtctt cgttggcacc
    16081 gacaacagca tttttcacat cttgaacctt gcggtctgca ataattttga tgttttctgg
    16141 caagccgact ggtccgaggg aaccaaaacc tgccccgaag attttttccg catccgctgg
    16201 gcctgctaca tcaaagaaat ttgctccaag gtggtttttc aacttgacat cgttaacctg
    16261 atcattgcca acaaggaggg caacaactgg ctcaccgtct gccataaaga gcatagtttt
    16321 gatagtttgc tcttcagcga cattcaagaa ggccgcaact tcgtcgatgg ttttggcatc
    16381 tggcgtcgct actttaacca attcttcttc gacgacaact gctgtacttg atttgtactc
    16441 gctagttgcc atttctaggt tggcagcata accagattcg ctagagtagg caatggtgtc
    16501 ttcaccagac acagaccatg ccaagagttc ttccttgatt gcttcaagaa catcctctgg
    16561 gatttcttca aaagaagcaa cagacttgtc caagacaacc caacggtcca ggtcagtacg
    16621 gtctggtgta atagccataa actcctggct atccttacca cccatggcac caccgtcacc
    16681 gataatggcc ttaaactcca agcctgcacg agtgaagatt gcctcgtaag ccgccttgta
    16741 gtcatcgtag gtctggtcca gactctcata gctagcgtgg aagctgtagc catctttcat
    16801 gatgaactcg cgaccacgga gaagaccgtt acgagggcgt ttttcatcac gatacttaga
    16861 ttggatttgg tagatgttaa gcggcaactg cttgtaagac tggaccgcat cacgcgccaa
    16921 aagagtcacc gtttcttcat gagttggtcc caagataaag tcagaaccct cgcgattttt
    16981 gagcttataa aggtcatcac cgtaggtttc ataacgacct gactcacgcc aaatatccgc
    17041 ggacaagagg gctggtgcca aaaattcaat agcatcaatc ttgtcaaact cctcacgcat
    17101 gatatttttt gctttttcaa tcacacggtt agccaaaggc aagtagctgt aaataccagc
    17161 agaaatctga cggacatagc ccgcacgtaa catcaaagcg tggctaatca ccgacgcatc
    17221 cgaaggcatc tcccgcaaag tagggataat cattttagac tgtttcatag ttaagatata
    17281 agtgcgttaa gaaaacgaaa cgaaaatagg aaacctaacg cagtgctttt ggcactaggc
    17341 aggtttatct ttttcgtaaa gtttttagca cgcattcaat tcctttctta tattcatcat
    17401 caggaaatcc agaggcgtag ctctcaatct attttccgag tttttagacc gagttcaatt
    17461 acgaacctcc ttaattcctt tcatcttttg tttatcagga atgcacaggt ctcagcccgt
    17521 gttcaattcc tttcataaat ttcaaattat cttagaagaa gactcttata atatcattcc
    17581 aagtgactac tatcataaga accaccataa cggctacacc tgctagagtg atataagatt
    17641 ccgtctctgg tttgagtggt tttctgcgaa tggcttccag aatattcata acaatcttac
    17701 caccatccaa ggctggaatt ggaatcaaat tgaagattcc aaggttaatg gagagcgcag
    17761 ccattagccc caatacagaa accaaaccaa attcagctgc ttgagaactt actttataaa
    17821 tagcaaccgg tccccccaac tgcttaacat caaagtttgc aattagattc ttcaaagctg
    17881 ttacaaccct aagagctgtc gctcccgctt cttggaaacc accgataatt ttatcaacga
    17941 aaccagtttt caaaatcggt gaaataccaa ttcgataggc cccatctact tgttcagcag
    18001 taacggagac atgacggttt ttcccatcat gctcaacaac caaatcaaag cttggggctg
    18061 ttgttgcatc tgtcgctgct ttgctaataa tcgttgcaac ttctgtgtag ctgtctgtcg
    18121 tgttcccatt gaccgaaaga atcttgtccc ctgtgacaac acctgcagcc tgcaaagcac
    18181 caccttcagt aatacttact gcattgcttg atgggttggc aaccccgcct tgcatgaaga
    18241 acaaaagaat aaatactaga atccctaaaa taaagttatt catgggaccc gcaaagttgg
    18301 tcatcaaccg tccccagact gtcgcatttt gatactgaac atcaagtgga gcaatccgaa
    18361 cttctgttcc atcttcctct acaatcgtgg catcgtgatc aaccttatag gtcttgcttt
    18421 cttccaagac caagcctgtg atttccaatt tttcctcgaa gtcaaaactg gttacattca
    18481 ttggcaaact gagactatcc agttgcttac cagacaaatt aatccgcgtc acaacaccag
    18541 cttcattcag gctgaggctg gcaggactac ctgtcttaat ctctgtctta tcttcacccc
    18601 atccagccat tcgaacatag ccacctaggg gaaggatgcg gatggtgtaa agtgtcccat
    18661 ccttacctgt atgagcgaaa attttgggac ccatcccaat cgcaaattcc ctcacaagga
    18721 taccagcttt cttggcaaag tagaaatgac caaattcatg gacaaccaca atcacaccaa
    18781 atataaatat aaatgctaaa attcccttca taatacctcc ttaaaataat ccaaagaagt
    18841 gcatgattgg aaagacaaaa atcataccgt caaaacgatc caggataccg ccgtgtcctg
    18901 gaataatttt tccagaatcc ttcacaccaa aatggcgttt aatagcactt tctactaaat
    18961 ctccgaattg tgagaatatc gagaaaatca caactaacaa caacatcatg ccaaatgaat
    19021 agcccgccaa caaggactta tcgacaatca agaaaatgat agcaactacc accgcagaaa
    19081 gaataccgcc tgcaaagcct tcaaccgttt tattcggtga aaccttcggc aataattttc
    19141 tccgaccaat ctgacgaccg attaagtagg caccaatgtc tgttgcccag actaaacaga
    19201 gggcaaagaa gactttattg agcccatcca tacgagccag tatcaaacta tgaaagccga
    19261 taccaacata gaaacttgat gcaattggaa agactacatc tgaatagttg taatgaccga
    19321 tattaaaaac cgtcgaacca agcagtaaaa atactacaat agcgtaggct gtgtaatttc
    19381 catccgtcgg taaaaatgtc agataattct ggagcggaag agccaagact aaactggcca
    19441 acatagctaa gacaccctcg atagaatttg gtgctaggcg atgcatttta atcaattcag
    19501 ccgtagcaat gatggctaac aggcctacaa aaaattgaaa tgccatccca ccagccaaca
    19561 aaaagggaat gaaaatagct agagcaacgc caccaaatat cactcgtttc tgtaaatcat
    19621 ttgtcatacc ttctccttat acaccgccaa aacgtctgtg gcgacggttg tattctttaa
    19681 tcgctaattt aagtgcttct ccatcaaaat caggccaagc aatatctgta aagtacagct
    19741 cgctgtaggc tgtttgccac ggcaggaagt tgctaagacg aagttcacca cttgtacgga
    19801 taactagatc gggatcgcgt aacgttggtg ataaggttcc tgtatagagg taatccgcta
    19861 ttaatttctc atcaatatcc ccaggattga acttggcatc caagacatct tgcgcaatag
    19921 ccttgactgc gcgagtcacc tcatcacgac cgccatagtt aagagcgaaa ttgagaatca
    19981 aaccagtgtt actctttgtc ttttgttcgg ctttataaag cgcattgaga gtcgggacag
    20041 gaagttttga atggtcacca atcatctgaa ttttgacatt attggcatgg agttcaggaa
    20101 catacttatc ataaaactcc acaggtagat tcatgataaa cttcacttct ttttccggtc
    20161 gagaccagtt ttctgtagaa aaggcataga cagttaacac ctttactccc aaatcagaag
    20221 ccgtcttggt aaccttttga agtgcatcca taccagcctt atgccccata atacgtggct
    20281 gcatccgctt tttcgcccaa cggccattgc catccataat cacagcgata tgcttaggga
    20341 cttcaattgc agtttcaatg ctttctttct ttttaaaatt aaatttaaac atggtatttt
    20401 ccttattgta taatcgtttc attataccat atttttacct aggatagcaa aaatcaggca
    20461 gggctaaaac aagagaaaac caaggtttcc ctcggttttt catccttatt cttcaattgc
    20521 agcttctgga gttgcttctg tcgtttcaac tgccacttct tcaacaactg cagatgtagt
    20581 acctgaactt gctacacgat tacgaatagc accacgttca aatgtcaagt aaacaccgtc
    20641 aacatcgaga acaacctttt tatcatcgat ttcatcgaca ataccaaata gcccaccaat
    20701 ggtcacaatt tcatcacctt ttttgatttg atttagtgct tcttgacgtt gtttttgttg
    20761 tttacgttgc gaatagaaca taaaaccaat catggcaacc atgacaagcg gcaaaaataa
    20821 accttccatt tgtttacctc ttttattgtt tgataagcat gattatatca aattctggac
    20881 aagaaagcaa agttcgacca aacagaaaaa gaccgaactt tcgttcagtc ttcgcacact
    20941 attttgcgct ccatacactc tcaaggatgt tggtctgttc acgaccaggt cctactgaga
    21001 aggttgagat acgaacgcct accaattcgc tgatacgacg aacatagttg cgagccgctt
    21061 ctggcaattc atccaggcta cgtacacctg tgatgtcttc agaccaaccc ggcatttctt
    21121 cgtagattgg tttgcaacgt ttgagttgct ccaaacttgc tgggtagtgg tcaatacgct
    21181 caccatccaa gtcataagcc acgcagattt tcaaggtctc aagacctgac aagacgtcaa
    21241 tcgagttgag ggacaagttg gtaatacctg acacacggcg actatggcgc atcaccactg
    21301 agtcaaacca accgacacgg cgtggacgcc cagttgtcgt accgtattcc ttaccgattt
    21361 cgcggatacg gtctccgatt tcatcgtgca attcagtcgg gaatggtccg tcaccaacac
    21421 ggctagtgta ggccttacat acaccaacaa ccttatcaat cttgcttggg ccgacaccgc
    21481 taccgatcgt cacaccacca gcaactgggt ttgaagaagt aacgaatgga taggtacctt
    21541 ggtcaatatc caacataacc ccttgcgcac cttcaaataa gacacgcttg ccttgatcga
    21601 gggcatcgtt caagataaca gaggtatctg ttacatactt tttgatttct tgaccataag
    21661 cgtagtattc ttcaaagatt tcgtcaaact caatcggagt tgactcatac attttctcaa
    21721 acaaacggtt tttctcagcc aagtttgttc tcaaacgctc cgcaaaaatc tccttgtcca
    21781 agaggtctgc aatacggata ccgacacgcg cagccttgtc catgtaggct ggaccgatac
    21841 ccttgttggt tgtaccaatc ttattctcac ctttagactc ttcttgcaac tggtccaatt
    21901 tgatgtgata aggcaagatg acatgtgcgc ggtctgaaat ccgcaaatta tctgttgtca
    21961 cacctgaatc atggagatag ttgatttcct tcaccaaaga ttttggattg acaaccacac
    22021 cattaccgat tacagaaatc ttttctggga agaagattcc tgacggaatc aagtgcaatt
    22081 tatacttcgt gccatcgata acgatagtgt gtccagcgtt atcaccacct tgataacgtg
    22141 cgattacttc agcattagca gacaagaagt ctgtaatttt acccttacct tcgtcgcccc
    22201 actgggttcc tacaacaact actgatgtca tattcactct ttctctgaac tggctaacca
    22261 gcccagcctt tcttctactt ctggcaggaa tctcacctgc aagtttcgtt ttacacacta
    22321 ttctatcaaa aaacgaacat tttttcaaga aattccaata atttcatata tatcgaatgt
    22381 ttttattttt ttaaaactat attttaaaaa aatccgatta aacacacgag gtagacatgt
    22441 gtaaatatgc ttttggcaga gaaatccgaa gtctttgttt actttcagtg attttcagta
    22501 catttttcac cagaaacgta gcagaaattt gtatttgctc tccaatctag ataaaattgg
    22561 aataagactt tgacagaaat ataaaggact tagatatgtt acaaaaatta tcaccaaaca
    22621 gccccattct tcaggctact tttggaattg agcgcgaatc acttcgcatc aattcaaacc
    22681 acagagttgc acaaactccc catcctcata aactgggttc tcgtagcttc cacccctata
    22741 tccagacaga ctacagcgag ccacagttag agttgattac accaatcgct cagtcgacga
    22801 aggaagcgcg tcgtctactg ggagccatta cagatgttgc cgcacgttct atggataagc
    22861 aagaatacct ctggccccta tccatgccac ctgtgatttc agaagaagaa atccaaattg
    22921 ctcaactaga tagcgactat gagtaccaat atcgtgtcgg tttgggggaa cgctatggca
    22981 agctcgtcca gtctatgtcc ggcatccatt ataattttga attaggaaaa gacttaaccc
    23041 aacaactttt tgaattaagc aaggaaacag actttatcgc attcaaaaat accctctacc
    23101 tcaaattagc acaaaacttc ctcaactatc gttggctctt gacctaccta tatggagcaa
    23161 gtagcctagc cgaaaaagga ttcctaacca cggaagtcgg ttgcgtccgt tccattcgta
    23221 attccaagta cggctatgtc aactccgatg atgttcatat ttccttttct tctctcgaac
    23281 agtacgtggc cgacatcgaa caagctgtcc aatctggtca actatccgct gagaaggaat
    23341 tttactcttc cgtccgacta cgtggagcaa agactagccg tgactacctc agcaagggga
    23401 tttcttattt agaattccgt tccttcgacc ttaaccccta tgacccgctt gctattagtc
    23461 aagaaaccct tgatacagtc cacctcttta tcttatctct tctttggcta gaccaactga
    23521 cagatgttga taacacccta gcaaaagcag ataaacttaa caatctcatc gccctcagtc
    23581 acccccacac acctctacct aacgatgccg atgccacgcc aatcttgacg gccatgaaag
    23641 ccatagttct acactttgga ctggatgact actatggcca gctcattgca catgtagagg
    23701 cagcacttca agaccctcgt ttgacccttt ctggaaaaat agcagagcag attgaagatg
    23761 ggtctctaga aaagttcggc cagcagcaag gccgactctt ccatgattat gcttggacag
    23821 ccccttacgc ccttaagggc tatgaaaaca tggaactctc cacccagatg attctctttg
    23881 acgccattca gctgggcttg aatgtggaga ttttagacga ggaagaccag tttctcaaac
    23941 tctggcatgg tgaccatgtc gagtacatca aaaatggtaa catgacctcc aaggacaact
    24001 acgtcattcc tctcgccatg gccaacaagg tcgtgaccaa gaaaatcttg gaccaggctg
    24061 gttttccagt acctgcggga gctgaatttg caaacaagac agacgccctg cggtattacg
    24121 gacaggtagc cagctctgct attgttgtca aaccaaagtc cactaacttc ggtctcggca
    24181 tctctatctt ccaagaacca gccagccttg cggactatga aaaagccctt gacatcgcct
    24241 tttcagaaga cagccatgta ctagtggagg aattcgtggc aggaaccgag taccgtttct
    24301 tcgtcctgga cggcaagtgc gaggctgtcc tgctccgcgt cgcagccaat gtcgtggggg
    24361 acggtagctc aaccatccgt gaattggtgg accaaaaaaa ccaagatcca ctgcgtgggc
    24421 gtgaccaccg ttcaccgctg gaaatcatta acttgggcga catcgaactg cttatgttgg
    24481 agcaacaagg ctacacaccc gatacagttt tgccagaggg cgttcaagcc tttctgcgtg
    24541 gcaattccaa tatctcaaca ggtggtgact ccatcgatat gaccgaccag atggacgagt
    24601 cctacaagca actggccgct gatatggcga ctgctatggg agcttgggct tgcggtgtgg
    24661 acttgattat ccccgaccgc accaagccag ccagcaaaga caagcccaac tgcacctgta
    24721 ttgagctcaa cttcaaccct gccatgtacc tgcacaccta tacttacgca ggacctggac
    24781 agagcattac gccgaagatt ttgaaaaaat tgtttccaga gctatagttt tccattttcc
    24841 ttactttgtt agtacttact tcaaagattc gtggagcttt tgaaggctgg agatagggca
    24901 aagcgagttc gcctcaatag cggcaggttg ctagaacatt ctaggttgga aataagactt
    24961 gcctctctat aaagtacggc aaagcgagtt caaacaaacc agtggattgt ttgaagtggg
    25021 aaagccagaa acggggttcc tcttgcgtct aagtaaaaaa gtcaactaaa ggactatata
    25081 tctgctttgc ccctttgttc cttcacgaaa actcccaact aatgttaaac aaaacgaata
    25141 agacaccgag ataatcattt atatctcggt gcttttttgt gaactaattc ccatcactca
    25201 accgttttgt tcgcctttta ttttgttttc caatttattt ttattatttc atttgatttt
    25261 ttgaaaatat ccgtattata tctaaaaaaa gtattccaaa cgactcattt tctgttaaac
    25321 tatatgtata taacaactgc agacgttttt atgacgtcca tatataacaa aaaggagttt
    25381 tatatgaaga ttagaaaccg ttctctcttc tataccgtag gttctgtagc ggtaacagcc
    25441 ggactcttgt ttagtcttgc tacttcgccc ataccatctg tgcacgctac tgaagtggcg
    25501 atagagaatt atccgtcact tgcaataacc aagtcggaag tcacgataca aggctatctt
    25561 attgcaccac ttaatagtag cggaactgct tttgacgcta ctaacaaaac aaatctagcc
    25621 cttggtgcaa gcatggatac tgcggccgcc gacactatcc ctattcaact aaaagaacct
    25681 cttcgtagcc aattcaacct cgttgatcat cccgaactag tcggaaaatt agttcgtatc
    25741 acaggaacta gcgatacata catgaagcga gctgggatca agccagctac agcgattgaa
    25801 attgtagatt catcttctac taatgttcag ccaccaacaa gtgaaaatac agctaccaaa
    25861 ccaagtgacc ttgtttctac accgattgct actgttcgtt ccggagcaca aggaacagag
    25921 tacacagttt ctggtaaaat tattagccta gtaaacggtt ggggaggaaa tggtttttac
    25981 ctccagggtt ctgacggtgc tggtatctat atctacccag gggctgcctt gggctatcag
    26041 cctggtgata cagtccaatt aacaggaaca ttgggtgagt ataaaggcga actccaacta
    26101 accacagtta gcaaccacaa ggctatctct gagaatttca acactcctat tacagaaaca
    26161 aacatcgctc agttagcaac gcaagcacag gcaacactag tttctctaaa aaacttgact
    26221 gtcggagaga ttcaaagcga tagctatcaa aattctacct ttaccgtaac tgattccgag
    26281 ggacaaactg tggatgtgcg tttagatagt cgaacaggca tcaaaacggc ggatctttta
    26341 aaccggatca acaagggaga taagattaat ctaaccgcta ttttatccac ctataatgga
    26401 aaaatacaat taaaaccatt tgacctctct cattttgaag tcattgaaaa ggcaacgacg
    26461 gaagcaggac taggaaagac cgaagctgta acagtcggtc gcattcaagg tgcaagtcac
    26521 cagtcacctc tggtgaatca atctgttatg cttaaaaatg tcgtagtaac ctacgttacc
    26581 tctgccaata acttctacgt gcaagatgtc acaccagatg gagacatcaa aacatctgac
    26641 ggcattaaca tctttactga caaattaaaa acaaatgtca aggtaggaga tcttgtcact
    26701 atcgcaggac gagtagaaga ataccagggc agagggtatg ctgaacgtga caaaacagac
    26761 ttaaccatca cacaaattcg agctactgaa gtaactgtga atgggactgc acctgtacca
    26821 tctcctatag tccttggtct tgatcggacc atcccagcag atattattga caacgacggt
    26881 ttggctcaat ttgatccaga acaagatgcg ctcgatttct gggaatccgt tgaaggcatg
    26941 gtggttgccg tggatgatgc aaaaatcctt ggcccactaa aaaacaagga aatctatgtc
    27001 actcctgcca ctagtcaact tcctttaaat aacgttggag gcgtcaacct tcgccctgaa
    27061 gggaataata ccaatatcat tccactcctt ttgaaaaatg gaaaacagat cgtcaaatca
    27121 ggagactatt tcaccggtcg tatcgctgga ccagttacct actcttacac caattataaa
    27181 gtctatgtag atgatagcac ccttccaacc ttgcacgaag gagctacaaa accagaaaca
    27241 acaaccatta tcccaaatga tgacaagctg actatcgctt cttataacat tgaaaacttt
    27301 tctgctgaca gtaagtcaac atcagacgct aaagtccaac gcattgccaa atcctttgtt
    27361 tcagacctac attctccaga tgttatcgga ttgattgaag tgcaggacaa taacggtgct
    27421 acaaatgacg gcacaactga tgctagtaag agtgctgagc gcctaattgc agccattcaa
    27481 gcagctggcg gaccaaccta tacctatgtg gatattgctc ctgaaaataa caaagacggc
    27541 ggacaggaag gtggcaatat tcgcgtcggc ttcctctaca actcaaaacg tgtcagccta
    27601 tctgacaaac caatcggtac cgcaacacag gcagttgcat gggaaaacgg cgaactcaat
    27661 ctcagcctgg gccgaatcga cccaaccaac cctgcttggg cagctgtccg caaaacactt
    27721 gcagctgaat tcgtcttcaa aggcgagaaa gtcgttgtcc ttgccaacca tctcaactca
    27781 aaacgcggtg acaatggtct ctacggaaaa atccaacctg tcagcttcaa gtccgaagaa
    27841 aaacgccact cactagcaca aattatcact gactttacaa aagcaggttt aactcaaaat
    27901 ccaaatgcta atatcgttat gctaggcgac ttcaatgact atgaatttac taaaaccata
    27961 gaaattttag aaacgggagg aatggctaac ctcgttagtc gtcacgatgc atctgatcgc
    28021 ttctcttact tctataacgg aaacaatcag tctcttgaca atatgctagt atcaactaac
    28081 ttgttggacc gctatgcctt tgatatggtc catgtcaatt cagcctttat ggaagaacat
    28141 ggcagagctt ctgaccacga tccgctattg gtacaactgg atgtaacaaa ggcacaagaa
    28201 ccaactcaac cagaaccaag cgataagcag acagatgact caggaacagt caataatagt
    28261 gatgacaatg gtacgaccaa caataacaag ccaacaaatc tttcaacttc taatcaaaca
    28321 gctgtaaatg ctgatgaccg ttctgccgct acagacaagg gacaaataac cgtgacccct
    28381 actgccaaca acagtcaaaa gaaaatcctt ccaaagacag gaggagaaac aagctttgta
    28441 cttatcacaa tcggtctcgt attcttgagc gcctgccttg tcaaaaaaca aaaagaatcc
    28501 taatcatttt ttgagcttga aagcatgact ttcaggctca tttttctact gctccacctc
    28561 cacttgtgct ataattaaag ttatggataa aattattaaa acactatcaa agagcgggca
    28621 tttccgtgcc tttgtgctag atagcacaga aaccgtgaaa acagcccaag aaaaacacga
    28681 caccatggcg tcgtccaccg ttgctctggg gcgcaccctc attgccaatc agattttggc
    28741 tgccaatgaa aaaggcgaca ccaagatcac cctgaaaatc ttggccagcg gtgctgttgg
    28801 ggctatcatc tccgttgcca ataccaaagg tcaggtcaag ggctacattc aaaatccaga
    28861 cttggattac aaacggacag cgactggcga agtcatcgtt gggcctcttg tagggaacgg
    28921 acaattcctg gttatcacag actacggaac aggtcatcct tacaactcca tgacaccctt
    28981 gatttctggt gaaatcggtg aagactttgc ctacttccta acagacagcc agcagacacc
    29041 gtctgcggtg gggctgaacg tcttgttgga cgaagaagac aaggtcaagg tagctggtgg
    29101 cttcttgttg caagttctgc caggtgcaac cgaggctgaa attgcccgtt tcgagaaacg
    29161 catccaagaa atgcctgcca tttcgagttt gctggcttct gaaaaccata tagaagctct
    29221 gctatccgct atttatggcg acgacgactt caaacgactg tcagaagaag aaatcggttt
    29281 tgtctgcgac tgctctaaag accgcttcct tgatgctcta gctagtctgc caaaaaccga
    29341 cttgcaagag atgaaggaag aagacaaggg cgtggacatc acctgtcaat tctgccagac
    29401 ccattaccac tttgacgaaa acgatttgga ggaactcatc aatggctaat ctcaacaccc
    29461 ctttcatgat tggtgatgtg gaaatcccca atcgctgcgt gctggcccca atggccggcg
    29521 tgaccaactc ggccttccgc accattgcca aggaaatggg cgcaggcctg gtcgttatgg
    29581 aaatgatttc tgaaaaaggc cttctctaca acaacgagaa gaccctccat atgctccata
    29641 ttgacgacaa cgaataccct atgtccatcc agctttttgg cggcgatgcc gaaggactga
    29701 aacgggcggc cgacttcatc caaaccaaca ccaaggctaa tatcgtagat attaacatgg
    29761 gctgccctgt caacaaggtc gttaaaaacg aagctggtgc caagtggctc aaggaccccg
    29821 acaagattta ccacatcatc aaggaagtga cctcagtcct cgatattccc ttgactgtta
    29881 aaatgcggac gggttggaac aataccgacc tagcggtcga aaatgccctg gccgcagaaa
    29941 gtgccggtgt gtctgccctg gccatgcacg gacggacccg cgagcagatg tacacaggaa
    30001 ccgttgacct ggaaaccctc accaaggtgg ctggcagcct gaccaagatt cctttcatcg
    30061 caaacggcga catccgcaat gtcgaagatg ctcgtcaacg tatcgaggaa gtcggagctg
    30121 atgccgtcat ggtcggacgg actgccatgg gcaatcctta tatcttcaac caaatcaacc
    30181 actatcttga aactggagaa gtcctacccg acctctcctt tgaagataaa ctcgaagtcg
    30241 ccttcgacca cctgacccga ttgaccaatc taaaaggcga atccatcgcc gtacgtgaat
    30301 tccgtggtct tgccccacac tacctccgtg gttcagcagg tgctgccaag atccgtggcg
    30361 ccgttgcacg agcagagacg attgaacagg tccaagaact ctttaaccaa gctagagaag
    30421 cttatcaaga acggattgct aaataagaaa ctctgagaag tttgatactt gccaaccatc
    30481 aaacatatat aaaaacgcat aaaaccaagg ttataacctg attttatgcg ttttttctat
    30541 actgttttac atagtttcct ttccaaaaaa ggcaagaaag gctggagcaa tgtaatcctg
    30601 atattgttga ggttgttcac gaatgagggt cgctgaaata ccaaattttc taggactgaa
    30661 atgcgcttga tagcctcgac tgtgcagttc ctgagcgtat tcttcctcag acacatagaa
    30721 aactggtagt tgatgttctg acaagtccaa taaaccaagc aggcggtcta gccaaaggtc
    30781 ccaaccatct ggatagggag gcaagtccgt ttcatccagg gcacgaactt cgacaaaatc
    30841 atcttcctta aactgctctt gagcaaattg aaatcgtttg gtcaggtcta aacctatcct
    30901 atccccacga tccctatcat agcctgaaac caccacacaa acctgaccac aggccaactt
    30961 tgctcgctcg atcaaatcca aatgtccctt gtgcatgggg gcaaaagttc cgaaaacaac
    31021 agctatggtt tgattcatag atttcctttc tacttgctaa agaggcgagc aagctcacct
    31081 ctccaactat ttcatccttt tttcaaaacg gatgaacaat tctgcatctg gaaaggatag
    31141 caacacaagc actccttatc cctttaccac atcaaacttg agctcttcac ccttgacccc
    31201 aaccttcaag gtcgaactag tagtcaagtc accacggagt aacatttctg ccaacggatc
    31261 ttccaatttg gtttgtaaga gcctacgtag cggacgagcc cccatttctg ggtcataacc
    31321 ttgtttagcc aataacttaa gggctgacgg ttgcagttta agagtaattc ctttctcagc
    31381 tgttacagcc aacaaaggtt taatcatgac cttgacaaca tcctgcatat gagtttccgt
    31441 taggctatgg aagacaactt tctcgtcaat acggttgata aattctggac gataggcttt
    31501 tttcaattcc tcaaaaatgc gtttctccac ttccttgtgg tccttagata aatcaagggc
    31561 accaaaacca actgtcttat catcacgaag agctgttgct ccaaggtttg acgtcatgat
    31621 aataatagta tttgagaaat caaccttacg tcccttactg tccgtcagaa caccatcatc
    31681 caagacctgc aagagaacat tgaaaatatc tggatgagcc ttttcaacct catcaaaaag
    31741 aagaacagaa taaggtttgt tacgaacttt ttctgtcaat tcgccacctt cttcatagcc
    31801 cacgtaacct ggaggcgctc cattgagacg gctggccgca aatttctcca tatactcgga
    31861 catatcaaaa cggataagag cagattcatc atcaaagaga acctctgcca aggctttagc
    31921 aagctctgtc ttaccgacac cagttggtcc caagaacata aaggaaccaa tcggtctgcg
    31981 acctgtacga atccctgact gattacggcg aatagctctg ctaactgcag aaatagcttc
    32041 ttcctgccca atcacacgct tgtgcaattc tgcttccaga ttcagatact tcttagtatc
    32101 tgattggcta agcttggcta ctgggatacc tgacaagcgg ctaagtgttt ctaaaacatc
    32161 ttcttctgtt acttcaaggt catagttttg actagcctcg gcatcttctt tcgtttcaat
    32221 caaaagcttg ctgacagtct tatatttttt atccatcaag gccttgtcta tgctcgtcag
    32281 tcctgtttcc tcaccctgcc ctttcactcg attttgtacc gttgcactgg cttcatccag
    32341 caagtcaatg gcagaatctg gcaggttttt actggtcaaa taccgcttag cgtaggtcac
    32401 agcagtctct acagctgctt gtccaatacg gaccctgtgg tatttttcaa aagtaccttt
    32461 taaaccagtc aaaatggcaa tgctatcttc cactgtcggc tcttcaatgg tcactttggc
    32521 aaaacgacga accagggctg cgtctttctc aatatgcttt tggtactctg tctgagtcgt
    32581 cgctccgacc gtacggaggg tgccccgagc aagagctggt ttgaggatat tggctgcatc
    32641 caaggtcgaa tcaatccctg agccagaacc cataatggta tgcaactcat cgatgaaaag
    32701 gatgacctga ccatcttcct cgatgtcgtt gatgatatta ttcatccgct cttcgaaatc
    32761 accacggaaa cgagttccag cgataacgtt catcaagtca agttccaaaa cccgcatctt
    32821 agccaagctg acagggacat cacctgcagc aacacgttga gcaagaccca aagcaagcgc
    32881 agtttttccg acaccagcat caccaactag aactgggtta ttcttggttt tacgtgatag
    32941 aatttggatc atccgtgcaa tctcagcgtc tcgaccgatg acaggttcaa tcttcccttc
    33001 acgcgccaaa gctgtcaaat cacgagtgta gtcttctaat ccaccacttt gaggtgctgg
    33061 gggcattccc atcatctgtc ccatattagt aggtttggct ttgccaccct tcatcacact
    33121 gcggatagct ttaaggtcct ctttggtaaa tcccgcacgt gcctccaagt ttttacgcaa
    33181 atccaagaaa cgaaacttat catctgagtc ttcaaaacaa aaacctacct tgtccaaaat
    33241 ctgactagct gttgaccgct tgtccaacaa catagccata aacaaatgtt ccgttcccag
    33301 ctccttagcc ttgacgactt ctgcaatttt cttggcaaaa ctagccgttt catccaaacg
    33361 tttagaagat ggtaaaatac gaaaactatc caactcttca cgatagacct tgtcggtcac
    33421 aacaaaagtt gcatgttcat agtcagaaat tgaaatagga tattctgcca aaacagcgcc
    33481 agcgactgta tcatggttga taacgaaaga caagagaaca tgccaggttt ccaagtaatc
    33541 acaggcataa cgttgagcca ctaactgggc atcttcaaaa acccgctgta aaccagttga
    33601 aattttcatg aattattcct ctttcctatc taaacgtcgt agtatttgac gcatcatgcg
    33661 tgctcgaata gtacttgcat cttttcctaa tatttcatca gatcctgtga acaaaaagag
    33721 attgccttct cgttctgtca tcaatccttc atcaaaaagg agctgtaaaa tatccgcata
    33781 aaccgtagca gacaactgct cacccatatt gtttaacaaa tcatggacca gttcgtgctt
    33841 atccgaaaag gtaatccgcc caattcggat ataaccacca ccaccacgct tactctcaac
    33901 aatatagcca cggctggctg taaaacgggt tttaatgacg tagttaatct gactcggtac
    33961 cacttcaaat cggctcgcaa gttcgctccg ttttagctcc gctacactca cctgttccaa
    34021 gagagccttg atatgctcct cgatataatc cgacgtgttt ttcatacact tccctttcta
    34081 actagactga ctatttttga ctattgtctt attataccga aaaatcaccc atttggaaag
    34141 caaagacacc gtttctacaa cgtatcaatt ttttgctttc tcccatcaaa tagcttatac
    34201 taaacctatt acaatgaaat ggagaatacc ccatgtttga taccaatcat ttttttgaag
    34261 atatcaaaga tttcaaagct gtctcaccaa aggaagcaag cgagcttctc gctgctaaag
    34321 acggtgccat cctattccta ggtcgcgaca cttgcccata ctgccgtcgc ttcgcaccaa
    34381 aattagctgc cgtagctgct gctcaaaact ggaccgttca cttcttgcat acacaagatc
    34441 ccgcctattc tgcacaagaa atcgctgcac tacgtgaaca ataccatgtt ccaactgtat
    34501 caggccttct cgttgcgaaa gcaagcggcg ttgacgttcg ctgtgatagc agcctaacag
    34561 aagacgaaat tctcgccttt gtaatggcct aatcgtttaa aatagtacca aaaaagagct
    34621 gagtaatgac tcggctcttt tcatgttgga gatgatagga ctattacgct tctttcgatt
    34681 gacgatagaa gataacagct cctgcaccga ctactaggaa tataacacca aggacagatg
    34741 tccaaagtcc tgacttctca ccagtcttag gaagaatttt tcctgttgta gtggttgaag
    34801 ctggtacttc tggaactgtt gtttcaactg tagtagttga agccggtgtt tcaggagtag
    34861 tcgttgtttc ttctgaagtt gtagttgtag tcgtcgttgt tgtagttgtt gtgatcactt
    34921 tattgataaa gttcacttct tgttctgatt tatcaactgt cgcaacaagt gttggaccac
    34981 cgttatctgt aacagttaca ataacgtcat actctacttc atcatagtcg atacctgcat
    35041 ctgtaccaag tacttctttt acagtgtact tgtatgtacc tggtgcatca aatgtcaatt
    35101 catcgaatgt aaccacacca gtgccatcgt ttttagcagt tgaaacaact gaaccgtcgt
    35161 ctttcttcaa gagagtgaat tcaaactctt gatctgcaag tgcacggcct tccaatgttt
    35221 tcgttgcttt caaaacaact ttaactggac cagctggttt tttcttgttg acaatttctt
    35281 taagaacact cttcgtaaca ccatcaaatt ccgctggatt gatgaccata tcttcgattg
    35341 gaaggtaacc atctggcgtc ttgatttctt taagagtgta ttcatctctt agaagattgc
    35401 taatgctacc ttcaccttga tcgtctgtag taattttacc aacactttgg cctgtacggt
    35461 tacggataac atcgaactca acatctgcca atggagcacc tgcttcatcg gtcttcttaa
    35521 ctttaatagt aaatacataa ccttcggctt taccacctgc tgcaaggtaa gtatatcccg
    35581 atgtaacggt ttgatcaata ccattggtag taatcaatgt tgcttcattg ctaaactctt
    35641 ctccatcaac agggacatac ggtgcttttg ttttataaat aatttgaatc ccgtcagtcg
    35701 gagccaaatc tggcaactca atactaaagg atttgccgtc tggtgcaaat gttagtgtca
    35761 cttgatcagt aatcgtttct atattatcca gtacccaatc gccggcttta aaactccagt
    35821 ttcccttctc aactctcaat gtatctggaa tatactctaa ttgcttgctt tttaaaacat
    35881 caaccacctt gacatctttc atactttggc cagaacggtt gattgcaata atgaaccatg
    35941 catcttgtgg gttctttgaa tcactccaac cacttttttg aatgtctcgt ttatctggtg
    36001 ttccaattcc ttggaattga attttaccag catggataat ctttccttgt actgtaatat
    36061 caagtggaat ttcttttttc tgctgaataa ctctatgatc tacacgaaca taaaaataga
    36121 atgacccttt tatatccgaa tgttcttcaa catactttgt ataagtcaat ttgattttct
    36181 tttctggctc tggagtgatt gtcgcattcg caacaacgtt accctctttg tccgtaattt
    36241 ggaaatcaga actttggtta aatatcagtt cagaaggcaa ttcaatttct atcacatctc
    36301 cttctttaac cgcattgttt ttgatatcaa aatcagcatt cagacgaaat tgctgccact
    36361 gttcgacatt tggtggcgtt aattcattcc catccttatc tgtaaagtac atttttgtaa
    36421 taacatttgt ggtctcgtcc ccatacacat gtgaactcgc ccccaaataa ccactaaaca
    36481 gaatcatgac tggaagtagg atgaaagcaa gtattttctt catctttgtc ataacaaact
//
LOCUS       DBNFEG010000004        35041 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.004, whole genome shotgun sequence.
ACCESSION   DBNFEG010000004 DBNFEG010000000
VERSION     DBNFEG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 35041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35041
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.004"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(93..284)
                     /locus_tag="AC1PNG_000099"
                     /pseudo
     CDS             complement(93..284)
                     /locus_tag="AC1PNG_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
     gene            complement(542..1477)
                     /gene="dapA"
                     /locus_tag="AC1PNG_000100"
     CDS             complement(542..1477)
                     /gene="dapA"
                     /locus_tag="AC1PNG_000100"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIP6700645.1"
                     /translation="MSIQDLRDVKIITAMITPFKEDGSINFEVLPELIEHLLSHHTEG
                     ILLAGTTAESPTLTHEEELELFGAVQKIVNGRVPLIAGIGTNDTRDSIEFAKEVAAFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVEMTPETMLRLAE
                     HPNIIGVKECTSLANMAYLIEHKPEDFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMYEMFTAIEQQDIRTAAAIQRKFIPKVNALFSYPSPAPVKAVLNYLGFEVGPLRLPL
                     VPCPEEDAKRIIKVVVDGDYEATKATVTGVVRPDY"
     gene            complement(1550..2626)
                     /locus_tag="AC1PNG_000101"
     CDS             complement(1550..2626)
                     /locus_tag="AC1PNG_000101"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIP6700646.1"
                     /translation="MAYVVAVVGATGAVGAQMIKMLEESTLPIEKVRFLASARSAGKT
                     LQFKGQDIVIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGAVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLERIIVSTYQAVSGA
                     GMGAILETQAQLRSVLNDGVEPKAVEANILPSGGDKKHYPIGFNAIPQIDLFTENDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKGIAPIDEVKAAIASF
                     PGAVLEDDVANQIYPQAINAVGSRDTFVGRIRKDLDKENGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(2795..3889)
                     /locus_tag="AC1PNG_000102"
     CDS             complement(2795..3889)
                     /locus_tag="AC1PNG_000102"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HIP6700647.1"
                     /translation="MKVFASPSRYIQGKHVLFQGAEAIGKLGTKPLILCDDLVYGIVG
                     EKFLSYLVEEGMQVYRVAFNGEASDKEIQRVVEIGKEQASDVVIGLGGGKTIDSAKAI
                     ADLLGVPVVIAPTIASTDAPTSALSVIYSEEGAFERYIFYKKNPDLVLVDTAIICQAP
                     PRLLASGIADGLATWVEARAILQSNGTTMAGGGQTLAGIAIAQTCEQTLFEYGLQAMA
                     SCEAKVVTAALENIVEANTLLSGLGFESAGLAAAHAIHNGFTALEGDIHHLTHGEKVA
                     YGTLTQLFLENRPKEELEKYIRFYQALNLPTTLEELHLADAGYEDLLKVGQQATIEGE
                     TIHGMPFAISAEDVAEALMAVDYYVRSLDK"
     gene            complement(4010..4966)
                     /locus_tag="AC1PNG_000103"
     CDS             complement(4010..4966)
                     /locus_tag="AC1PNG_000103"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIP6700648.1"
                     /translation="MKQFSGLSRTIQGNHLLDTCAIDILKIGHRPLVLCAKQEYNTTV
                     ESFVKVLTCHGLIVKIVYMEEWDQNSVEECGEVGFRHRADHIIGIGNSSVAACTKAVA
                     DLLDLPAVLIPTSPFTTLPWVSVENKKRERPLFNESIQLIIADREQLLTQPTSELING
                     VVNTLLYLAAIKEVKRNHLFSLVDDILIRSFESVLLRGSLQAIEDFEARILSKEVQDV
                     LEVLSTCVTLDLPSDAWDVIQQIESKLSERREDLVDEPLKRNVVLYLLLSYLTQESEE
                     FQARLKWCQQLLLSANSSNLLSLEAVLKEIALEMGEDGSTRC"
     gene            complement(4998..5666)
                     /locus_tag="AC1PNG_000104"
     CDS             complement(4998..5666)
                     /locus_tag="AC1PNG_000104"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HIP6700649.1"
                     /translation="MEFMLDTLNIEEIRKWAEVLPLAGVTSNPTIAKKEGDIDFFERL
                     HLIRDIIGPNASLHVQVVAKDYEGILADAKKIRELAPENVYIKVPVTPAGLAAMKTLK
                     AQGYQITATAIYTVFQGLLAIEAGADYLAPYYNRMVNLNIDSNAVIAQLSEAIDRECS
                     ESKILAASFKNVEQVNQAFANGAQAITAGADIFEAAFSMPSIEKAVNDFADDWSAIHG
                     RYTI"
     gene            complement(5681..8125)
                     /locus_tag="AC1PNG_000105"
     CDS             complement(5681..8125)
                     /locus_tag="AC1PNG_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HIP6700650.1"
                     /translation="MKTVTEVAGTSIKTEYFGSLTDRMNKYREDVLDKKPYIDAERAV
                     LATKAYQEHREKPNVLKRAYMLKEILENMTLYIEEESLIAGNQASSNKDAPIFPEYTL
                     EFVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWEKNNLRSRAGVLLPEEVQVYMET
                     GFFGMEGKMNSGDAHLAVNYQKVLEQGLRGFEERVRAAKANLDLTDPASIDKYHFYDS
                     IFIIIDAVKAYANRFVALATELAEKAPTAQRRQELLEIARICAKVPYEPAETFAEAVQ
                     SVWFIQCILQIESNGHSLSYGRFDQYMYPYVNADLEAGRETEDSIVERLTNLWIKTIT
                     INKVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTV
                     RYHAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIEKGVLEEDAYDYSAIGCVET
                     AVPGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPAFGHFKDMKSFEELETAW
                     EKTLRHLTRMSVIVENAIDIALEREVPDILCSALTDDCIGRGKHLKEGGAIYDYISGL
                     QVGIANLSDSLAAIKKLVFEEGRLTPEELWHALETDYAGERGKEIQEMLIEDAPKYGN
                     DDDYADQLVTAAYDIYIDEIAKYPNTRFGRGPIGGIRYSGTSSISANVGQGRGTLATP
                     DGRNAGTPLAEGCSPSHNMDKNGPTSVLKSVAKLPTHEIVGGVLLNQKVNPQTLAKEE
                     DKQKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQLHPEKHRDLIVRVAGYSAFFNVL
                     SRATQDDIIGRTEHTL"
     gene            complement(8277..9578)
                     /locus_tag="AC1PNG_000106"
     CDS             complement(8277..9578)
                     /locus_tag="AC1PNG_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIP6700651.1"
                     /translation="MSKIDTQKIIVPIMKFVNMKGIIALKDGMLAILPLTVVGSIFLI
                     LGQLPFEGLNAAIASIFGAEWTEPFMQVYSGTFAIMGLISCFAIGYSYAKNSGVEPLP
                     AGVLSLSSFFILLKSSYIPVDGEPIGDAIAKVWFGGQGIIGAIIIGLVVGAIYTTFIQ
                     RHIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYIVSKMATNGGTFIEMIYDVIQV
                     PLQGLTGSLPGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKALLAADKLS
                     LENGAHIVTQQFLDSFLIMSGSGITFGIVFAMLFAAKSKQYKALGKVAAFPALFNVNE
                     PVVFGFPIVMNPVMFLPFVLVPILAAFIVYGAISIGFMQPFSGVTLPWSTPAIISGFM
                     VGGWQGAVVQIIILAVSTIVYFPFFKIQDKIAYESETKEPV"
     gene            complement(9591..9896)
                     /locus_tag="AC1PNG_000107"
     CDS             complement(9591..9896)
                     /locus_tag="AC1PNG_000107"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIP6700652.1"
                     /translation="MVKIALFCAAGFSTGMLVNNMKIAAADAGVEVEIDAHSQGKIAD
                     YLDEIDVALLGPQVAYTLDKSKALCDEKQVPIAVIPMADYGMLDGKKVLQLALSMVS"
     gene            complement(9929..10252)
                     /locus_tag="AC1PNG_000108"
     CDS             complement(9929..10252)
                     /locus_tag="AC1PNG_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIP6700653.1"
                     /translation="MEMIVADQLIMGLIIYAGDAKSHAYQALSLVKAGEEERAEEELE
                     LADAALLEAHNMQTKFLAQEAGGTKSDITALFVHSQDHLMTSITEINLIKEIIDLRKE
                     LKKVG"
     gene            complement(10440..11414)
                     /locus_tag="AC1PNG_000109"
     CDS             complement(10440..11414)
                     /locus_tag="AC1PNG_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HIP6700654.1"
                     /translation="MKQEKRRQLAKIAYLYYEEGKSQAEIAAETGIYRTTVSRMLTKA
                     KSEGIVKIEIQDFDRRLYRLEKYVQEKYGLKGLELVENSTDEDQLDLENRLAQAAADM
                     LTNLIDDGKKVGFSWGRSLSLIVDKIGNHRMKGVKFVPIAGGPSHIHARYHVNTLIYD
                     MANKFRGECSFINATIIQENQELAQGILSSKYFEELRADWKDLDVAAIGIGGRVDEKN
                     RQWLDMLTTADFQALSDEGAVGETCCRGLNQYGQAIVEDLQKRTIAISLDDLKEVPDT
                     IALAYGEEKAQAILAVLRAGYIHHLVTDERTIVKVLELDNDVHVHTNL"
     gene            complement(11427..12170)
                     /locus_tag="AC1PNG_000110"
     CDS             complement(11427..12170)
                     /locus_tag="AC1PNG_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIP6700655.1"
                     /translation="MERLDKIIQLVSQHEKIDVNSLAEQLDVSKVTIRKDLDKLESKG
                     LLRREHGYAVLNSGDDLNIRMSFRYDVKKRIAKEAANLIADNETIMIESGSTCALLAE
                     EICKTKRNVTIITNSYFIANYVRQYDSCQIILLGGEFQKDSQVTVGPLLHKMIQFFHV
                     DKAFVGTDGYDSEHGFTGKNLMRSEVVQYMSDVAENMIVLTDSSKFTKRGTVRRFGLS
                     QVAQVITDTSLSEDLVKELENARVTVRLV"
     gene            12302..13078
                     /locus_tag="AC1PNG_000111"
     CDS             12302..13078
                     /locus_tag="AC1PNG_000111"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HIP6700656.1"
                     /translation="MNTRKGIIFNIQHFSLHDGPGIRTTVFLKGCPLRCPWCANPESQ
                     KRKPEPMLDAVSKKMTIMGEEKSVDDIISEVMKDIDFYEESGGGLTLSGGEIFAQYEF
                     AKAILMEAKRHGLHTAIETTAFVEHHKFVDLIQYVDFIYTDLKHYNTISHRKVTGVNN
                     NLIIQNIQYAFSIGKEIVLRIPVIPDFNNSLEDAEQFACLFNQLKIDKVQLLPFHQFG
                     ENKYKLLNRTYAMTDVKALHPEDLKDYQAIFIKHNINCYF"
     gene            complement(13375..13662)
                     /locus_tag="AC1PNG_000112"
     CDS             complement(13375..13662)
                     /locus_tag="AC1PNG_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HIP6700657.1"
                     /translation="MAKVYEFEAVIHPVPDKGGAYIIFPYDIREEFGKGRVKVHARFD
                     GHPYDGSIVNMGVKDEAGNICYIIGVQKATRLAIGKQAGDRVQVTIQERME"
     gene            complement(13673..14326)
                     /locus_tag="AC1PNG_000113"
     CDS             complement(13673..14326)
                     /locus_tag="AC1PNG_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIP6700658.1"
                     /translation="MKINELEKRLLAVADASQAQPMKAYMKNHFDFLGVRTPDRRKVA
                     RQFFKESKAQGIDWEFVEACWSKPYREFQYIAIDYLVTKKKDLVLADLPRLKKLAQSK
                     SWWDSIDGLDKLVGKIVLDNPEAKQTILEWSLDDDFWLRRIAIDHQLLQKEKMDTELL
                     EKILVNNLNQIEFFINKAIGWSLRDYSKTNPDWVRAFLKKYSSQMAGLSIREASKYI"
     gene            complement(14316..14825)
                     /locus_tag="AC1PNG_000114"
     CDS             complement(14316..14825)
                     /locus_tag="AC1PNG_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIP6700659.1"
                     /translation="MIDIRSARIEDAADLVAIYAPYVEKTAITFETEVPTVEAFASRI
                     EKTLEKFPYLVSVEEGKVVGYAYASTYYARAAYDWTVELSVYIKQEARGKGIGSLLYD
                     AIEEDLKARGFKNFLACIALPNPASLSLHKKRGYEQVAHFKNVGYKFDTWHDIVWLQK
                     SLVGKQNED"
     gene            complement(14826..15443)
                     /locus_tag="AC1PNG_000115"
     CDS             complement(14826..15443)
                     /locus_tag="AC1PNG_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5052 family protein"
                     /protein_id="HIP6700660.1"
                     /translation="MKLQKKIMLSVVLGLSLVSLSACQSIANWWKNTKEEWIGLEMTV
                     RTYDENSQLIDEMSGRSLSISRNQEFDSVDAEGYSNADSSVLKVTLGNYEIDHVGSSL
                     IAAEEGLEDLYAKYQTTVDIANYDRAIPLVNRMVSSLKNDFTGKAKVVLIRSQNGTPL
                     ATYVGDKVSLYASDAPKTSELLIDGKRLIIYRCDYTIYDRELLEN"
     gene            15712..16452
                     /locus_tag="AC1PNG_000116"
     CDS             15712..16452
                     /locus_tag="AC1PNG_000116"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HIP6700661.1"
                     /translation="MDGKYPLFFLTISLVALYVFPMILIIRYIAKRFDISKKVIHLSW
                     ILGITASFYFSGLGQTLLGAFWLFIVKPPQTFIQNWGAAVTAPFHEEFGKGLVVLLVL
                     LLLKKLTLKNAVVSGMVVGLSFQIIEDGLYVFQQIFKSKADGFATLIERMLHAGGTHW
                     AFSLAFAVGLVALVSKNSGMSKKQGLFWMLMAVLAHFFLNTPFNEGLTSNSGEITVLM
                     LCFSFCVALAAFFKVDQIETNQHTSNTY"
     gene            complement(16699..18010)
                     /locus_tag="AC1PNG_000117"
                     /pseudo
     CDS             complement(16699..18010)
                     /locus_tag="AC1PNG_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631733.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
     gene            18227..19036
                     /locus_tag="AC1PNG_000118"
     CDS             18227..19036
                     /locus_tag="AC1PNG_000118"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HIP6700662.1"
                     /translation="MKEKFQMCKTYLPVVLATIGFVNGCKIIFGELGKPNGMEAKYPL
                     FLLTISLVAIYILPLLVLTYSLAKRYNISKQVIGLSWLLGLTSSVSFSELGHTAIGYF
                     LLEIVKASNDFLNDWGAAISGPLAEEIGKGLTVLLVLLICRKMTLKNALVSGVIVGLG
                     FQIMEDITFVFRDMFMNKLDGFETILERVGQAGWAHWVFTLLFAIGLVALLTKNTGMS
                     KAQGVFWIGASFGIHFLFNSPFNTGIFQTVFPILSISLGLLAYQTVEKLSE"
     gene            complement(19080..20750)
                     /locus_tag="AC1PNG_000119"
     CDS             complement(19080..20750)
                     /locus_tag="AC1PNG_000119"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIP6700663.1"
                     /translation="MKTDIEIAQSVTLKPITEIVEKVGISFDDIELYGKYKAKLSFDK
                     INAVKDNAPGKLILVTAINPTPAGEGKSTVTIGLADALSKIGKKTMIALREPSLGPVM
                     GIKGGAAGGGFAQVLPMEDINLHFTGDMHAITTANNALSALIDNHIHQGNAVGIDQRR
                     IIWKRVVDLNDRALRKVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDINDLKER
                     LANIVIGYRFDRSPVYVRDLAVEGALTLILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALRLADYTVTEAGFGADLGAEKFLDIKVPNLPKSPDAVVIVATHRAL
                     KMHGGVAKTELSAENVEAVKAGFSNLKRHVENIQKYGIPAVVAINEFVSDTADEIAAL
                     KELCAEIGVPVELASVWANGADGGVELAETVVATIENQAANYQRLYKAEDSLEEKVTK
                     IVTQIYGGSGVVFEKKARNQLTEFAKNGWDKLPVCMAKTQYSFSDDQFALGAPTDFDI
                     TVREFVPKLGAGFIVALTGDVMTMPGLPKAPAALNMDVAADGTAIGLF"
     gene            21254..21949
                     /locus_tag="AC1PNG_000120"
     CDS             21254..21949
                     /locus_tag="AC1PNG_000120"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HIP6700664.1"
                     /translation="MKLLITSGGTSEAIDQVRAITNHASGNLGKIIAEQALKRGHEVT
                     LVTTRQAVKPDSQKNLTIIEITNVDSLKETLEPLVKSHQALIHSMAVSDYTPVYMTGL
                     DELRATEDSSSLLKKQNTESKISSKDDYQVLFLKKTPKVISYVKKWNPAITLFGFKLL
                     VNVPKEELFAVARQSIERNGADYILANDLKDIGENQHIAYLVDKTREIQAQTKDEIAQ
                     LILNTLEKGEQNG"
     gene            21942..22490
                     /gene="coaC"
                     /locus_tag="AC1PNG_000121"
     CDS             21942..22490
                     /gene="coaC"
                     /locus_tag="AC1PNG_000121"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HIP6700665.1"
                     /translation="MANITLAVTGSISAYKAADLTSQLTKLGHQVTVLMSRSAMDFIT
                     PLTLQSLSKNLVHTDVMIEENPTLIKHIDIAKETDVFLVAPASANTIAKLANGMADNI
                     ITATALALPIGTKKLLAPAMNTNMYLNPATQKNIKTLIEYGFEEIKPREALLACGDFG
                     TGALAEVTDILEKVSNTLDEKK"
     gene            22477..23046
                     /locus_tag="AC1PNG_000122"
     CDS             22477..23046
                     /locus_tag="AC1PNG_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIP6700666.1"
                     /translation="MKKNKSTQIATIAIFFAVMIVINILSSIIFNILPVPIKPTIVHI
                     PVIIASIIYGPRVGACLGALMGLMSMTHNTIILLPTSYLFSPFVENGNVYSIIIAVVP
                     RILVGITPYFVYKWIKNRKGLFIAGAIGSMTNTIFVLGGIFFLFSSVFDGNIQAMLAT
                     VLGTNSIAEMIISSVLTAALVPTLQKIQK"
     gene            23137..24855
                     /locus_tag="AC1PNG_000123"
     CDS             23137..24855
                     /locus_tag="AC1PNG_000123"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIP6700667.1"
                     /translation="MTYQETYQTWLDFADLPDYLREELVAMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINVFVVRQATEGLAKLVESKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAAHGIKSYVFESLRPTPELSFAVRHYNAIAGIMVTASHNPKEFNGYKVYGEDG
                     GQMPPADADALTNFIRAIDNPFAVELADLEASKENGLITVLGEETDVKYLEELNDLNI
                     NPELIAEYGKDMKIVYTPLHGTGEMLARRALAQAGFESVQVVEAQATADPDFSTVASP
                     NPESQAAFALAEELGREVGADVLLATDPDADRVGVEVRQADGSYWNLSGNQIGAIIAK
                     YILEAHKQAGTLPANAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLMVAEIAAYYRSRGMTLADGIDEIFKE
                     YGYFAEKTISVTLSGKDGAEQIKAIMAKFRDNSPAQFNATDIAVFEDFALQTKTDKDG
                     NIEKLTTPPSDVLKYTLADDSWFAVRPSGTEPKIKFYIATVGETLAEAEEKISNIEKE
                     INEFVG"
     gene            complement(25018..25549)
                     /gene="tnpA"
                     /locus_tag="AC1PNG_000124"
                     /pseudo
     CDS             complement(25018..25549)
                     /gene="tnpA"
                     /locus_tag="AC1PNG_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(25953..29036)
                     /gene="ide-Ssuis"
                     /locus_tag="AC1PNG_000125"
     CDS             complement(25953..29036)
                     /gene="ide-Ssuis"
                     /locus_tag="AC1PNG_000125"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-specific IgM cysteine endopeptidase
                     Ide-Ssuis"
                     /protein_id="HIP6700668.1"
                     /translation="MNIQERFSLRKSAVGLVSVSLLCAIYTSTVAADTVVTGVNEIIE
                     ESQVKDEVSIESEKNESLDGSNIEIVEEIADNIPSPVIAEGEVAVEMKVDRGTENVVS
                     RNDTEVTTSEQNQIEVTETKEILNQTSYQTESGEQRQIIWAHGITPPAMEQSGGFVKE
                     KYGDYLNYTAPFKAGKGYYDTNKSLNASFIDLNLCFAAVSSNMVHWWLEQNSSYVERY
                     LKEKKGTVNVGENYAITDLRRYIDSFQDQQNSRVFDMFKTYYGYRTNGFVSDALVDLF
                     INGYKPKVQGGVNLEDSQLVPDSRGGFFYDVFKEKKLTNRIFSGSYERFGEDVRTVLE
                     SKGLLGLTYRTLGYATHIVTVWGAEYDNQGKIRAVYITDSDDQQEQIGLKRMGITRDA
                     SGNPRLNNHVKNNSAGALLDYVHTIRLGQDLWEEYFNPFAKAKEIASQILADRKKALV
                     LSIQGQSELPESMRLIYLEKLNNLYNQGILSIQKTESSEMLSGALENGLNSLKSLDFP
                     ISEVGNALAPDLSVGDRSTVSDVDSLSSQETSSTNLEADTENAGIIADGTNQLHFPVE
                     AQTTSSVEAEGDNVFEQEADTLPIIIENKDEFGSELSGNMQTSETDSLVVAVEEDVKN
                     DEVDQVEKLLESEKVENQSSELLSDTLIVEGANDKEEDRVEAVVSEQPDSIPHQNVEI
                     SPVEPMNVETESVVTPINDAATPHGFPMYIDNSVTESVAIPLEKDSIQAGETEIAEPT
                     SSESTSVEAELVDNSEIHSATSSVTPRGSSAYADSSTTESVATLLEKDSIQAGETEIA
                     EPTSSKSTNVEAASVDNSEIHADTSLTAVSSVNLDNPVIEPVAIPLIGSKRDTNAEVE
                     VSSLSKREVRKPNTEGLISVQSKVIKKELLESSLVEAGSPLLEATIAQSSNSNSTEIG
                     MSYQNTVLLESNNTERQVSKAEIVIEHKETELVETVSSASEPVVLVENISQTSNNTIE
                     SGKNMGVQSQAGAKQILGIEQSSKVSTPTSRQIMGVGLLTLVLGSALGLLKKRRK"
     gene            complement(29197..30702)
                     /gene="pyk"
                     /locus_tag="AC1PNG_000126"
     CDS             complement(29197..30702)
                     /gene="pyk"
                     /locus_tag="AC1PNG_000126"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIP6700669.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGDDGYWGEKLDVESSAQKIAA
                     LITEGANVFRFNFSHGDHQEQGDRMATVRRAEEIAGKKVGFLLDTKGPEIRTELFEDD
                     AKEYAYTTGDKLRVATKQGIKSTKEVIALNVAGGLDVFDDVEVGKQILVDDGKLGLTV
                     VEKDAVNREFVVLVENDGVIAKQKGVNIPYTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVEVVRNICKETGNDHVQLFAKIENQQGIDNIDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIITKVNAAGKAVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIAKNAQTLLNEYGRLNSDQFNRASKTEVIASAVK
                     DACHSMDIKLVVTVTETGYSARSISKYRPDADILAVTFTEKVQKSLMLNWGVIPIVTN
                     RPDSTDDMFDLAERIAKEQGLVEAGDDIIIVAGVPVGVAGSTNTMRVRTVK"
     gene            complement(30769..31779)
                     /gene="pfkA"
                     /locus_tag="AC1PNG_000127"
     CDS             complement(30769..31779)
                     /gene="pfkA"
                     /locus_tag="AC1PNG_000127"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIP6700670.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVYGINEGYAGM
                     VAGDIHELSARSVGDIISRGGTFLGSARYPEFAKLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGVPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRHAGDIALWAGIASGADVIVVPEEDFNINDIVDRIKAGYDNGKKHSII
                     VLAEGVMPAAQFAEELKAAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGRGGLAVGIRNEQMVENPILGTAEEGALFSLTTDGKIVVNNPHKADLELADLNRNL
                     SI"
     gene            complement(31864..34974)
                     /locus_tag="AC1PNG_000128"
     CDS             complement(31864..34974)
                     /locus_tag="AC1PNG_000128"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIP6700671.1"
                     /translation="MLAQLDTKTVYTFMDSMVTIENYVGRAKELGYHALGIMDKNSLY
                     AAYSFMESCNKMGLQPIIGCELDWKLQEGEQDVTTQLIALTTRGYRNLLKISTAKMIG
                     QDDFETIRQYLVDIAVVVPYFSGIEHLELGVNYYIGVTVDTPVQDFKQAILPIHTVRF
                     FEEEQLESLQVLHAIRDNIPLNQVVDIDSRQKLLSADSLTNLFSNRFPQAVDNLRDLV
                     AGISYQLNTDLKLPRFNRDRPAVEELYERSYQGLEEKGLTNSLYKERLEKELSIIHQM
                     GFDDYFLIVWDLLRFGRSQGYYMGMGRGSAVGSLVAYALNITGIDPVKHNLLFERFLN
                     AERYSMPDIDIDIPDIHRADFIRYVRDRYGSTHAAQIVTYSTFGAKQAIRDVFKRFGT
                     PEYELSNITKKISFRDSLTSAYQRNAAFRQIINSKIEYQKAFAIAQQIEGQPRQTSIH
                     AAGIVMSDEDLTDTIPLKIGDDMLVTQYDAHAVEANGLLKMDFLGLRNLTFVQKMAEG
                     VRERYQQEIDIAKIDLEDKKTLELFAAGRTKGIFQFEQPGAISLLRRVKPGRFEDIVA
                     TTSLNRPGASDYSDNFVKRKHGQETVDLLDPTIAPILQPTYGIMLYQEQVMQIAQVYA
                     GFTLGKADLLRRAMSKKNAQEMQVMEAEFLQGALKNGHAEENAREIFGMVAKFAGYGF
                     NRSHAYAYSALAFQMAYFKSHYTDVFFDVMLNHSSGAYIEDAMQFDFEVARLTINNIP
                     YHDKFDKSKVYLGLKTLKGLPKDLALWILDHRPYKSVEDFILRLPDNFKKKDLLIPLI
                     QIGLFDDFVANRKKIIENLDNLFVFAEAIGTFFAEESYSWTDVEDYSDSEKFMLEQEL
                     LGVGISPHPLVNICRESKRSFTDLADLAVGNRATVLVQIQAIRLIRTKKTGEQMAFLR
                     VTDTKRTLDVTLFPESYRQYASILEEGGMGYITGRVQERDGQLQLVLEKLEPVSLEKF
                     WILLENREHDYAVARILDKYRGSIPVVLHYQDSNQTIQAEHHMVMKTPQLEEELSEFV
                     MKTIFR"
BASE COUNT        10214 a         7916 c         6302 g        10609 t
ORIGIN      
        1 ttgttttctc cataagacca gttcctccct tatttggtat ctacgtaata aaaaaacttc
       61 cctaaccatt gctgattaga gaaggatatt tcttaaaaaa tgagaccata aaggacagcc
      121 gcaagcaagc ctccgatgat tggaccaact actggtaccc aagcatagcc ccaatctgaa
      181 tctcccttat gcttcaatgg aaggatggca agcatgatac gaggaccaag gtcacgcgca
      241 gggtttaggg catatcctgt cggaccacca agagaaactc cgagtcaaaa caaaggttcc
      301 gattgcttcg ctaattgtaa atgtccccca gacattgttc gctctcttat ttcaaggctc
      361 acgttaaaat acttcgcttt tttattttca agctcagata agaatagtcc actggactat
      421 tcttatcccc taactatcct cgctttccag tttccaggct cgggtcttct atggtcactt
      481 tcatatttct tggtgaccag ctgaaacagt tccccgaact gtttcactcc cccagacatt
      541 tttaataatc cggtcttacg actcctgtca cagtggcttt agttgcttcg tagtcgccgt
      601 caacgactac tttgataatg cgtttggcat cttcttctgg gcatggaacc aacggcaagc
      661 ggagtggacc aacttcaaat ccaaggtaat taagaaccgc cttaactggt gcaggacttg
      721 gatatgagaa gagggcattg actttcggaa tgaacttgcg ttgaatagcg gctgctgttc
      781 gaatatcttg ttgttcgatg gctgtaaaca tttcatacat ctcatcacca ttcgtgtgag
      841 atgcaacaga aatcacacca tctgctccca agttcattgc atggaaagca tcgccatcct
      901 cacctgtata aatcaagaag tcttctggtt tatgctcaat taggtaagcc atattggcaa
      961 ggcttgtaca ctctttaaca ccgatgatat ttggatgctc tgccaaacgc aacatggttt
     1021 ctggtgtcat ttcgaccaca acacgccctg gaatattgta gataatgatt ggtaaatcag
     1081 aagcatctgc aatggccttg aagtgctgat acattccctc ttgagagggt ttgttgtagt
     1141 aaggcacgat agcaagccct gccgcaaagc cgccgaatgc cgccacttct ttggcaaact
     1201 caatcgagtc gcgcgtatcg tttgtaccga tacctgcaat taggggaaca cgaccattga
     1261 caattttttg cacagcacca aatagttcca gttcttcctc gtgtgtcagg gttggacttt
     1321 cagcagttgt tcctgctaat aaaatccctt ctgtgtgatg ggacaacaga tgctcaatca
     1381 actcaggtaa aacttcaaaa ttaatcgagc catcttcttt gaaaggggta atcattgctg
     1441 taataatttt tacatcacgt aaatcttgaa tagacatagg cttcctctct tactgattgt
     1501 tgctaaaaaa ggctgagtgc agccttttgt gtacttttgg ttaataaaat tatttcaatt
     1561 caaacttcaa ttctgccgtt ggacgaacta gaccacgctc atgaagggtt tcagcgattt
     1621 gaacagagtt ccatgcagcg cctttgagca agttgtcaga aacaacccac atgtgaatac
     1681 cgttttcctt gtccaaatct ttacggatgc gaccaacaaa agtatcgcga ctaccaacag
     1741 cattaatggc ttgcggataa atttgattag ccacatcgtc ttctaaaaca gcccctggga
     1801 aggatgcaat agctgccttc acttcatcaa tcggtgcaat tcccttagtt tcaatgtaaa
     1861 cagattcaga gtgagctgac aagactggaa tacggacgca ggttgcagaa acggcaatgc
     1921 tgtcatcttc catgattttc ttagtctctt ttgtcatctt catctcttcg tatgtgtagt
     1981 cattttcagt gaagaggtcg atttgcggaa tagcattgaa accgattgga tagtgcttct
     2041 tatcgccacc agaaggaaga atattcgctt ctaccgcttt gggttccacg ccatcattca
     2101 agacagaacg aagctgagct tgtgtttcca agatagctcc catacctgct ccagagactg
     2161 cctgataggt ggatacgatg atccgctcca agccccattt ctgacgaaca ggctccaagg
     2221 caaccatcat ttgaatggtt gagcagtttg gacaagcaat gataccattg tgggcatcaa
     2281 gcgcgtgagc atttacctca ggaacaacca gaggaacatc tggattttga cggaagtaag
     2341 aggtattgtc aactactaca gctcctgctt ttacagcgta aggagcgtac ttggcagatg
     2401 tagagccacc agctgagaag agggcaatat ccactccttc aaaggctgtt tctgttgttt
     2461 cttcaatgac aatatcctgc cccttaaact gcaaggtctt acctgctgaa cgggcagaag
     2521 caaggaaacg cactttctcg attggaagtg ttgactcttc caacatttta atcatttggg
     2581 caccaactgc accagtggca ccaacaacag ctactacata ggccatagaa atctccttta
     2641 agtcaattct tcaaaatttt ctaaatattt tatatttaac aattatatca taattttttc
     2701 aaatgaaaag ccctgagaaa gaatttccca aggcaattac ttcgaacctc tattcaccag
     2761 tttagatgga catgctcaac tgtgaatgct cttcttattt atctaatgag cgaacatagt
     2821 agtcaactgc catcagagct tctgcaacgt cttctgctga aatagcaaat ggcataccgt
     2881 gaattgtttc tccttcaatc gtcgcttgtt ggccgacttt caatagatct tcataaccag
     2941 catcagccaa atgtaactcc tccaaagttg ttggaagatt caaggcttga tagaaacgga
     3001 tgtatttttc caattcttct ttcggacggt tttccaagaa gagctgggtc aaggtcccat
     3061 aagccacttt ttcaccatgt gtcaaatggt gaatgtctcc ctccaaggcg gtgaatccat
     3121 tgtgtatcgc atgcgccgca gccaaacctg cactttcaaa tcctaaacca cttagcaagg
     3181 tattggcttc aacaatattc tctaaagcag ctgtcacaac cttggcttca cagctggcca
     3241 tggcttgaag tccgtattca aagagtgttt gttcacaggt ttgtgcaata gcgataccag
     3301 caagtgtctg acctcctcct gccattgttg ttccattgct ttgtaaaata gcacgcgctt
     3361 ctacccacgt cgccaagcca tctgcaatac cagaagccaa aagacgtggc ggtgcttgac
     3421 aaataatcgc tgtatctacc aagacaagat ctggattttt cttatagaaa atatagcgtt
     3481 caaacgcccc ttcttcagaa taaatgacag ataaagcaga tgtcggagca tctgttgaag
     3541 caattgttgg agcaatgaca actggcactc ctagtaaatc agcaatcgcc ttggctgagt
     3601 caatcgtctt accaccacct agaccgatta cgacgtcact agcctgttct tttccgattt
     3661 ctacgacgcg ctggatttcc ttatcagatg cttctccatt aaaggcaaca cgatagactt
     3721 gcatcccttc ttcaacgaga taggacaaaa acttctcacc gacaatccca tatactaaat
     3781 catcacaaag aatcaaaggc tttgttccca attttccgat agcttctgca ccttggaaaa
     3841 ggacatgttt accttgaata tagcgagatg gacttgcaaa tactttcata agaattcctc
     3901 cttgaaataa tagatattgt tatattgagc aataaacgac agttagcatt gccgaaagta
     3961 agaggtatga gcctgcattt taaatattgc tattgtacag cgatgcctac tagcaacgtg
     4021 tcgagccatc ttctcccatc tctagtgcaa tttcctttaa aactgcctcc agcgacaata
     4081 gattgctaga gttggcagac aaaagcaact gttgacacca cttcagcctg gcttgaaatt
     4141 cttctgattc ctgtgtcaaa taggacagaa gcaagtagag gacaacattt cgtttcaatg
     4201 gctcatccac caaatcctct cttctttcgc tgagctttga ttctatttgt tgaatcacgt
     4261 cccaagcatc actaggcagg tctaatgtta cacaagtaga aagaacttct aagacatctt
     4321 gtacttcttt tgataatatc cgagcttcaa aatcctctat agcttgaagg ctacctctta
     4381 ataaaacgct ttcaaaactt ctgataagaa tatcatctac caagctgaat agatgatttc
     4441 tttttacttc ttttatggct gctagataaa gaagagtatt gaccactcca tttatcaact
     4501 ccgaggtagg ttgtgtcaat aattgctccc tatccgctat aattagctgg atggactcat
     4561 taaacaatgg tcgctctctt ttcttatttt ccactgatac ccaaggtaga gtggtaaacg
     4621 gtgaagtcgg tataagaaca gctggcaagt ctagtaaatc tgcaacagcc tttgtacaag
     4681 ctgctacaga actattacca atgccaataa tatggtctgc acgatgtcgg aaaccaactt
     4741 ccccacattc ttcaaccgag ttctggtccc actcctccat atagactatt ttaacaataa
     4801 gtccatgaca agttaaaact ttcacaaaac tttctacagt cgtattatac tcctgttttg
     4861 cacataaaac aagggggcga tgacctattt tcaaaatgtc aatcgcacaa gtgtctaaaa
     4921 gatgatttcc ttggatagtg cgtgaaagac ctgagaattg tttcatagtg cccccttatt
     4981 ttataatata gagatgttta gatagtatac cttccatgaa tggcagacca atcatctgca
     5041 aaatcattta ccgccttttc aatggatggc atagaaaagg ctgcttcaaa tatatctgca
     5101 cctgccgtaa tagcctgcgc cccatttgca aatgcttggt ttacttgctc cacatttttg
     5161 aatgacgccg ctaaaatttt actttccgaa cactccctat caatcgcttc tgataactga
     5221 gcgatgaccg cgttcgaatc aatattcaga ttgaccatgc gattgtagta cggcgctaag
     5281 taatctgcac ccgcttcaat agctaacaag ccctgaaaaa cggtataaat cgctgttgct
     5341 gtaatctgat aaccttgcgc tttcaatgtt ttcatagcag ccaaaccagc aggcgttacc
     5401 ggaaccttaa tatagacatt ttccggtgcc aattcacgaa tttttttagc gtctgccaaa
     5461 atcccttcat aatcctttgc cacaacttgc acatgtagtg aagcatttgg accgatgatg
     5521 tcacggataa ggtgcaaacg ttcaaaaaag tcaatgtcac cttctttctt cgcaatcgtt
     5581 gggtttgagg tcacccccgc aaggggtaga acctcagccc attttcgtat ttcctctata
     5641 tttaaggtgt ccagcataaa ttccataata ataacctctt ttacaatgta tgttctgtac
     5701 gaccaatgat gtcgtcttgt gttgctctcg acaatacatt gaagaatgct gaataacctg
     5761 ctacacggac aatcaaatca cggtgttttt caggatgtag ttgtgcatca atcaaggttt
     5821 ctcgagagac aacattgtac tgaatatgat agccatgcaa gcggttaaag aatgtccgca
     5881 agagggcaat taatttttgt ttgtcttctt ctttggcaag tgtttgtgga ttgaccttct
     5941 gattgagcag aacgcctcca acaatttcat gtgttggtaa tttggcaact gatttcaaaa
     6001 cggatgtcgg tccattttta tccatattat gtgatggaga acaaccttct gccaacggtg
     6061 tacctgcatt tcgaccatct ggagttgcca aggtaccacg cccctgtcca acgttggctg
     6121 aaatggatga tgtacctgaa taacggatac caccaatcgg accacgacca aagcgagtgt
     6181 ttggatattt ggcaatctca tcgatataaa tatcatacgc tgctgttacc aattggtctg
     6241 cataatcatc atcgttacca tattttggtg cgtcctcaat caacatttct tggatttcct
     6301 taccacgctc accagcatag tctgtttcca aagcatgcca taattcttct ggtgttaatc
     6361 gtccttcttc aaagactaat tttttgattg ctgctagaga atcagataaa ttggcaatgc
     6421 caacctgcag accagaaatg taatcgtaaa tcgctccacc ttctttcaag tgcttaccac
     6481 gaccgataca gtcatctgtc agagctgagc agagaatgtc tggtacttct ctttctaaag
     6541 caatgtcaat tgcattttca acgatgacac tcatccgtgt tagatggcga agcgtctttt
     6601 cccatgccgt ttcaagctcc tcaaaggatt tcatatcttt gaagtgacca aaggctggtg
     6661 caaaccgttt gcctgatgca ggatcgatac catcattcat ggtaatcaat aaaactttcg
     6721 ggaagttcat atagctcata cctgtacaac ggtatcccca tttaccagga acagccgttt
     6781 ctacacagcc aatcgcactg tagtcatagg catcttcttc caagacacct ttctcaataa
     6841 aggatggaat gataatttca tcgttgttaa aggcaggcat accaaaacca agtttcatga
     6901 cttcaataca ttcattcatg aaacgagcat ctaggcctgc atgatagcga acagtcaagt
     6961 ttggctgtgg caggtgtgtt tgggcaacag atttcaatac taagtaagaa agtggattga
     7021 cagcatcctt cttatcacgt gtttgtccac caattgttac attttggtat agtggactac
     7081 cagcagatga gaaggtatgt gattgactac gaaccttgtt gatagtaatg gtcttaatcc
     7141 acaagttagt caagcgttcg acgatgctgt cttccgtttc acgtcctgct tctaaatcag
     7201 cattcacata cggatacatg tattggtcga aacgtccata ggatagagaa tgtccattcg
     7261 actcgatttg caagatacac tggataaacc agacagattg cactgcctct gcaaatgttt
     7321 cagcaggttc atacggcacc ttcgcacaaa tccgtgcaat ctccagtagt tcttgacggc
     7381 gttgagctgt tggtgctttc tctgccaatt cagtagccaa ggcgacaaaa cgatttgcgt
     7441 atgctttcac cgcatcaatg atgataaaga ttgagtcata gaaatgatat ttatcaatac
     7501 ttgcaggatc cgtcaaatcg agattagcct ttgctgcacg aactcgctcc tcaaaaccac
     7561 gcaaaccttg ttccaatacc ttttggtaat tgacagccaa gtgggcatcg ccagagttca
     7621 tcttaccttc cataccaaag aagccagttt ccatataaac ttgaacttct tctggaagca
     7681 agacacctgc acgtgaacga agattatttt tttcccagaa tggagcgatg ctgcgcaact
     7741 gctccttggt ttcctcggta atatagaaga cgtccccatc ccgtttttcg aataaatcca
     7801 attcattcaa tacaaactcc aaggtatatt ctgggaagat gggagcatct ttatttgaag
     7861 aagcttggtt acctgcaatc agactttctt cttcaatata aagcgtcatg ttctccaata
     7921 tttccttgag catataggcc ctttttaata cattgggctt ctctctatgt tcttgatagg
     7981 cttttgttgc taaaacagcc cgttccgcgt caatataggg cttcttatca aggacatctt
     8041 ctctgtattt gttcatccga tctgtcagac ttccaaaata ctcagtttta atgctagtgc
     8101 cagctacttc agtcactgtt ttcatagttt taccctttct ttcgaaactt aattttgttt
     8161 cgtttgtaaa ttaagtatat cctgtaatag gggttattgt caattgatgc tagtgagcgc
     8221 taagatttcc gaagccggct gcacatttgt gcaactcttt tgtctgacat cattgactaa
     8281 actggttctt tcgtttcact ttcataggca atcttatctt ggattttgaa gaatgggaaa
     8341 taaacaatag ttgagacagc taaaataatg atttgaacaa ctgcaccttg ccaaccaccc
     8401 accataaaac cagaaatgat tgctggagta gaccatggta gggtcacacc tgaaaatggt
     8461 tgcataaagc ctattgagat tgcaccgtag acaatgaaag ccgctaagat cggtaccaaa
     8521 acaaatggta agaacattac aggattcatc acaattggaa aaccaaagac gactggttca
     8581 tttacgttaa agagtgcagg aaaggccgct accttaccaa gagccttata ttgttttgat
     8641 ttagcggcaa atagcattgc aaaaacaata ccgaaggtaa tacctgaacc tgacataatc
     8701 aagaaactat ctaagaattg ttgggtcacg atgtgggcgc cattttccag agacaattta
     8761 tctgctgcta aaagggcctt attggcatcg aggtttgaca atagtaaagc agtaacgaca
     8821 ccattgacaa ctgactgacc atgtacacca aaccaccaga ggaaggaaat gaagaaagca
     8881 attccaatcg cacctggtaa tgaacctgtc aaaccttgta gtggcacttg aatcacatcg
     8941 taaatcattt caatgaatgt accaccgttt gtcgccatct tggaaacaat atagacaatc
     9001 atagatagca aaaagatgac aaaagctgga atcatagctt caaactgctt ggcaatagct
     9061 tgcggtactt gttcaggcat cttgataaca atatgacgct gaataaaagt cgtatagata
     9121 gcgcccacta ccaaaccaat aataatcgca ccgataattc cttgaccacc aaaccatact
     9181 tttgcgatgg catcaccgat tggttctcca tcaactggaa tataagatga tttcaagagg
     9241 ataaagaatg aagacaagga cagtacgccc gcagggagag gctcaactcc actatttttt
     9301 gcgtaagaat aaccaatcgc aaaacaagaa atcaagccca taatcgcaaa ggttccgcta
     9361 tagacttgca taaacggttc agtccattcc gctccaaata tagaagctat tgcagcattt
     9421 aagccttcaa aaggcaactg ccccaaaatt aagaaaatac taccaactac tgttaaaggt
     9481 aagatagcca acataccatc ttttagagcg atgatccctt tcatattcac aaatttcatg
     9541 attgggacaa taattttttg tgtatcaatt ttggacatga ccatactcct ttatgaaacc
     9601 atgctgagtg ccaattgaag aacttttttc ccgtccaaca tcccgtagtc tgccattgga
     9661 atcacggcaa tagggacttg cttttcatca cagagcgctt ttgacttatc aagcgtatat
     9721 gctacttgtg gtcccagtaa agctacatcg atttcgtcaa ggtaatctgc aattttccct
     9781 tgggaatgcg cgtcgatttc tacttcgaca cctgcgtctg ctgctgcgat cttcatgttg
     9841 tttaccaaca tgcctgtaga aaaaccagcg gcacaaaata gagcgatttt aaccatttcg
     9901 gtttcctcct aaatataatt tttttgtttt agccaacttt ttttagttct ttccgcaaat
     9961 caatgatttc tttaatcaaa ttgatctctg taatagatgt catcaaatga tcttgcgaat
    10021 ggacaaatag tgctgtaata tcagattttg ttcccccagc ttcttgtgct aagaattttg
    10081 tctgcatatt gtgagcttct aataatgctg catctgccaa ttctaattcc tcttctgctc
    10141 gctcttcttc acctgctttg acaagcgaca aagcttggta ggcgtgactt ttcgcatctc
    10201 cagcataaat aatcaatccc atgatcaatt gatctgcaac aatcatttcc atttcttttt
    10261 cctccgatgt gttttatttg taagttatta tatttctttc gtaagtaaat catatcattt
    10321 tctctttata gcgtaaatat atttatagaa cattttctgt tttaatttat ttctgcactt
    10381 ttgtgcattt ttggggataa aaaatacttc aaccaccttt aagcgattga agtactctct
    10441 tacaaatttg tatggacatg cacatcatta tccagttcta aaacttttac gatggttcgt
    10501 tcatctgtta ccagatgatg aatgtagcca gctcgcaata cagctaaaat ggcttgagcc
    10561 ttttcctccc catatgcaag agctatggta tctggaactt cttttaaatc atctaaagag
    10621 atggcaatgg tcctcttttg caaatcctca acgatagctt gaccgtattg attcagacca
    10681 cgacaacagg tttcaccgac tgcaccttca tctgataagg cttgaaagtc agccgtcgtc
    10741 aacatatcta accattgacg atttttttca tcgactcgac cgccaatacc tattgctgct
    10801 acatctaagt ccttccagtc agcacgtagc tcctcgaaat atttagaaga aagaatacct
    10861 tgagcaagtt cttgattctc ctggataatc gtcgcattga taaaactaca ttctccacga
    10921 aatttattgg ccatatcgta aatcagagta ttcacatgat aacgtgcgtg gatatgactt
    10981 ggaccgccag ctattggaac aaatttgaca cctttcatac gatgattccc tatcttatct
    11041 acaataagac tgaggcttct cccccatgaa aaaccaactt tctttccatc atcaatgaga
    11101 ttggtaagca tatcggctgc agcctgtgcc agacgatttt ctaaatcaag ctggtcctca
    11161 tcggtgctgt tttcaaccag ttctaaacct tttaatccat atttttcttg aacatatttt
    11221 tctaatcgat ataggcgcct atcaaaatct tgaatttcta tttttacaat gccttctgac
    11281 ttagccttgg tcaacattct actgactgtc gttctataaa ttcctgtctc agcagcaatc
    11341 tcagcttggc ttttcccctc ttcgtagtaa agataggcta tttttgccaa ctgccttctt
    11401 ttttcttgct tcatactgcc tccaacctaa actagcctta ctgtgactct agcattttct
    11461 aattccttga caagatcttc agaaagcgac gtatctgtaa tgacctgagc aacttgactt
    11521 aaaccaaatc tgcgtactgt ccctcgtttt gtaaatttac tagaatctgt taacacaatc
    11581 atgttttcag caacgtcaga catgtactgt actacttcgc ttcgcattaa gtttttacct
    11641 gtaaaaccat gctcactatc atagccgtca gttcctacaa aagccttatc aacatggaaa
    11701 aactgaatca ttttatgcaa gagcggccca actgttactt gggaatcttt ttgaaactca
    11761 ccacccaata aaataatctg acagctatca tattgtcgca cataattggc tataaaataa
    11821 gaatttgtta tgatggtcac atttctcttg gttttgcaaa tttcttctgc caagagagca
    11881 caagttgacc cagactctat cataatcgtt tcattgtcgg cgataagatt tgctgcttct
    11941 tttgcaattc gtttttttac gtcatagcga aaggacatcc gaatattaag gtcatcgcca
    12001 ctatttagaa cagcatagcc gtgttctcta cgtaataaac cttttgattc taacttatct
    12061 aaatctttac ggatggttac cttagaaacg tccagttgct ccgccaaact attgacatct
    12121 attttttcat gttgtgatac cagttgtata attttatcta atcgttccat ttcatcacct
    12181 catcattatt ctatcaaatt ttgactcttt ttcaactatt tgcaaccttt caacttacgt
    12241 tcgtaattgt aaaactttct tttgtgtgat aaaatcaaat taatcgaaaa ggagaaagag
    12301 catgaataca cgaaaaggca ttatttttaa cattcaacat ttttcgctcc atgacggtcc
    12361 tggtatacgt acgacggttt tcttgaaagg ttgcccccta cgttgtcctt ggtgtgcaaa
    12421 tccagaatct caaaaacgaa aacctgaacc catgcttgat gctgttagta aaaaaatgac
    12481 catcatggga gaagaaaaat ctgttgatga catcatatcc gaagtcatga aagacattga
    12541 tttttatgaa gaatctggtg gtgggctcac gctgtctggc ggagaaattt ttgcccaata
    12601 tgagtttgcg aaagctattc ttatggaggc aaaaagacac ggccttcata ccgctattga
    12661 aaccaccgct ttcgtagaac accacaaatt tgttgacctt atccaatacg ttgactttat
    12721 ctatactgat ttaaaacatt acaatacaat ttctcataga aaagtcaccg gcgtcaacaa
    12781 taaccttatc atacaaaata tccaatacgc cttttccatc gggaaagaaa tcgtcctacg
    12841 tatccctgtt atccctgact ttaataattc cctcgaagat gctgaacaat ttgcctgtct
    12901 ctttaatcaa ctcaagattg acaaagttca actactcccc ttccatcaat ttggagaaaa
    12961 taagtataag cttctcaatc gaacctatgc tatgaccgat gtcaaggctc tccatccaga
    13021 agatttaaaa gactatcaag caatcttcat caaacataat atcaactgtt atttctaact
    13081 ttgagaaata caaaaacttc ctcggctgag ccaatagcat tcctatagaa atcacatact
    13141 gaaatatgct tctgatagag tatctgaagc atacttagta tataaaaaag gcccaccata
    13201 aaggtgggcc agggcatcgt ttccgatgag aatagaaggt tcacacaact attcaaggtc
    13261 cgctcctgcg tttcaattaa atgaagacct ccctagcgtc gaaagtgaca aagtcactac
    13321 tcggctcatc gcatgaatct attctaccat aatctcaagg atttgcaagc ctgattactc
    13381 cattcgttct tgaatggtta cttgtactct atcaccagcc tgttttccaa tagccagtcg
    13441 agtagccttt tgaacgccaa taatataaca gatattgcct gcttcatcct taactcccat
    13501 attgacaatg gagccatcat agggatgacc atcaaatctg gcatgtacct ttaccctccc
    13561 cttgccaaac tcttctcgta tatcataggg aaagatgata taggctccgc ccttatccgg
    13621 tactggatga atgaccgctt caaattcata tacctttgcc ataacagccc tcctagatat
    13681 atttactagc ttcacgaatg gataagcctg ccatttggga actgtacttt tttagaaaag
    13741 cccgtaccca gtcaggattg gtcttggaat aatctcgcaa actccagcca atcgccttgt
    13801 taatgaaaaa ttcaatctga ttgagattgt tgaccaaaat cttctcaaga agttccgtat
    13861 ccatcttttc tttttggaga agctggtggt caatggcaat ccgcctcaac cagaaatcat
    13921 catccaaact ccattccaag attgtctgct tggcctctgg attgtccaaa acaatcttac
    13981 cgactaattt atccaagccg tcgatactat cccaccaaga cttactttga gccagttttt
    14041 tcaaacgggg taaatcagct agaaccaagt cttttttctt ggtaaccaga taatcaatgg
    14101 cgatatattg aaactcacgg tagggcttag accagcaagc ctctacaaat tcccagtcaa
    14161 ttccctgagc cttagattct ttgaaaaatt gcctagcaac ttttcggcgg tcaggagttc
    14221 gcacacctag aaagtcaaag tgattcttca tataggcttt catgggttga gcttgactgg
    14281 catccgccac agccaacaaa cgtttttcta attcgttaat cttcattttg cttacctaca
    14341 agagattttt gaagccagac aatatcatgc caggtatcaa atttataacc aacatttttg
    14401 aaatgtgcta cctgttcata ccctctcttc ttatgaagag aaagcgaggc tggattagga
    14461 agagcaatac aggctaaaaa gttcttaaag ccacgcgctt tcaaatcttc ctctatggca
    14521 tcatataaaa gacttccgat tccttttcca cgtgcttctt gcttgatata tacggataat
    14581 tccaccgtcc aatcgtaagc cgcacgggca tagtaggttg aagcgtaggc ataacccaca
    14641 actttacctt cttctactga gaccagataa ggaaacttct ccaaggtctt ttcaattcga
    14701 cttgcaaagg cttcgacagt cggtacttct gtttcaaagg taatagcggt cttttctaca
    14761 taaggagcat aaattgccac aaggtctgcc gcatcctcta tccttgcgga acgaatgtct
    14821 atcatttagt tctccaataa ttctctatca taaattgtat aatcacaacg gtagataatc
    14881 aaccgcttgc catcaattaa gagttcagaa gtcttaggtg catcagatgc ataaagagac
    14941 actttatcac ctacatatgt agcaagcggg gttccgtttt gcgaacgaat caaaaccacc
    15001 tttgccttac ctgtaaaatc atttttgaga ctagatacca tacgattaac aagcggaatt
    15061 gcacgatcgt aatttgcaat atctactgtg gtctgatatt tcgcatacaa gtcttccaaa
    15121 ccttcttctg ctgcaatcaa agatgagcct acgtggtcaa tttcgtaatt cccaagtgtc
    15181 accttcaaga cagacgaatc cgcattggaa tagccttcgg catccaccga gtcaaattcc
    15241 tgattccgcg aaatcgaaag ggatctacca gacatttcat ctatcaattg agaattttca
    15301 tcgtatgtcc ttaccgtcat ctccaaacca atccattctt ccttggtatt tttccaccaa
    15361 ttcgcaatgg actgacaagc agacaaacta acaaggctca aaccaagaac aacagataac
    15421 atgattttct tttgcaattt catataatag tcctccaaca tattttactt attatatcac
    15481 ggaatacaaa aaccgagaaa aaatagatag acctgagata ataaaaagat ggcaatgata
    15541 aactatagca cggtagggat aactgacatc tactaaatta atataaagga agtcattttt
    15601 atgaaacaat tctgtgctaa acacaaaatg aagctactta tttttctagt cgtttggagt
    15661 tttttctctg gctgtaaagt tcttcttacc ttttttggga aaccagatgg aatggacgga
    15721 aagtatcctc tcttctttct aactatttcc ttagttgcac tttatgtctt ccctatgatt
    15781 cttatcattc gttatatagc gaaacgtttt gatatttcta aaaaagttat ccatctcagt
    15841 tggattttgg gtataacagc tagtttctac ttctctggtc ttggtcaaac gcttttagga
    15901 gcattttggt tattcatcgt caaaccacct caaacattta tccaaaactg gggagcagct
    15961 gtcaccgctc ccttccatga agagtttggt aagggcctag ttgtactact cgtactccta
    16021 ttgctaaaaa aacttactct aaaaaatgcc gtagtcagcg gtatggttgt aggtttgagt
    16081 ttccaaatta ttgaagacgg tctctatgtc ttccaacaaa tctttaaatc taaagcagat
    16141 ggcttcgcca ctcttatcga acgtatgctt catgctggtg gaactcactg ggccttctcc
    16201 cttgcattcg cagtcggttt ggtcgctctc gtctcaaaaa atagtggcat gtcgaaaaaa
    16261 caaggacttt tctggatgct gatggctgtc ttagcacact ttttcttgaa tactccattc
    16321 aacgaaggac taacatctaa ctctggtgaa ataaccgttc tcatgctctg ctttagtttc
    16381 tgcgtggcac ttgctgcatt tttcaaagta gaccaaattg aaacgaatca gcacaccagt
    16441 aacacatact gaaacaagcc tgcattgaca ggcttttgtt atgttacaaa ccaagtattc
    16501 gaaatggttg ttgattcatc aatatctatt atcagtcttt atcccattcc atcggtccaa
    16561 gaaatcgcag ataattataa aaaaccgccc attacggacg gtcatagtca gtcaatgaaa
    16621 taaggtcgct cgatgatctt gacactagtc taggaaacgc agccacaact aatacaagaa
    16681 gcttatctcc ctagagtatt aaaaattaaa gacgtcattc aaattctcag ccatagtcgg
    16741 gtgagtgaaa atctgtgttt tgaagtagct ataaggaatc ttattgtcaa tcgccatagc
    16801 aatcaagtta atcagctcct ctgaattgcg accaaagaga gtcgccccca gaacaagctt
    16861 actttcctta tccacgataa ccttgaaaat gcctttgagg tcattattga catgggcacg
    16921 tggcatattg gcgacaggca attcgttggc aatgtagtcg taacctgctt ccttggcttc
    16981 cttctcggtc agaccgacac gagacagcac aggcgtaata aagacgctgg ttggaataga
    17041 cttacgttgg ctcaagctat aagttccagt cccagtcaac ttgccgaaaa cgatacggaa
    17101 gtcgtctagg gatgtgtaag tgaattgtgg tccgccattg acatctccca ccgcatagac
    17161 acctggaaca gtcgtttcgc agtagtcatc caccttaaca gcaccatttt ccaaaagctc
    17221 aatcgccgta ttttccaagc ccaagtcagc agtgttcggc acccgacctg tcgcatagag
    17281 gacggcgtcg aagattgccg tttcgccatt aaccgtcaca gcaacttgct cgcccgcagc
    17341 tgctacttgc tcgagtttcg cacccaagac aaaggtcacg ccagcttctt ccatatactc
    17401 cttggctaac ttagcaacca cttcctcttc tctcggtaaa atagcagagc tgacttcata
    17461 taccgtcacc tggctgccga gtttgctgta aagcccagca aattccaatc caatgttgcc
    17521 tccaccgata attgccaacg tgttaggacg agtttccaag ttctgaatgc ctgtgctgtc
    17581 ataaacgtga gctgtatcca acaagcctgg aattggaagc acgcgcgact tagcacctgt
    17641 attgatgacg atggtttccg cagttagttg aatggattcc tcaccagccg atacttccac
    17701 aaccttgtct gcaacaaaac gtgcgtgtcc ttgatacaaa ctagcaccac tgcctttcaa
    17761 gactgcctcg tttttgtttc gcagacgagt ggtgactgtt tctttttgat ccattacctg
    17821 ctcaaaagtt cagtttttat ctgctgctac caagagggtc ttggttggaa tacagccaat
    17881 attgatacaa gtcccgccaa acatagcagt attttcctca accaaggcaa cctttttccc
    17941 agctactggc agcttacctg ctaaggtctt tacatccaaa accaatgacc aataaatcat
    18001 attgttccat aacatactcc ttttattttt taaaccttag tctagcaaaa aagaaaattt
    18061 gagacacaat tccgttacgg ctctattatg gagtcctagt tgcaggaaaa aacattacaa
    18121 taaccgataa ggtttagata aaaaataggc gaattataga caaaatgtac tcatttttga
    18181 tacaatgtat ccatacacca gtttattaat acagaaaggt caaactatga aagaaaaatt
    18241 tcaaatgtgt aaaacctatc ttccagtcgt cttagcaacg attggatttg tcaacggttg
    18301 caaaattatc tttggagaat taggtaagcc aaatggcatg gaagctaaat atcctctctt
    18361 tctcctaacc atttccttgg tcgctattta catcctcccc ctactagtac tgacatattc
    18421 cttagctaaa cgctataaca tctccaaaca agttataggt cttagctggc ttttaggctt
    18481 aacaagtagc gtctcttttt ctgaactggg acatacagct atcggatatt tcctgctcga
    18541 aattgttaaa gctagtaatg atttcctaaa tgactggggt gcagctattt caggtccatt
    18601 ggcagaagaa atcggtaaag ggctaactgt tctacttgta ctgcttattt gtagaaaaat
    18661 gacactaaaa aatgcattgg taagcggagt gattgttgga ttaggtttcc aaatcatgga
    18721 agacatcacc tttgttttta gagacatgtt catgaataaa ttagacggct ttgaaaccat
    18781 tcttgaacgc gttggtcaag ctggttgggc ccattgggtt ttcacgcttc tctttgccat
    18841 tggcttagta gcccttctca cgaaaaatac aggtatgtca aaagcacagg gagtattttg
    18901 gatcggtgca agctttggaa tccattttct ctttaattca ccattcaaca ctggtatctt
    18961 ccagacggtg tttcctatct tgagtatttc ccttggctta cttgcctatc aaacggttga
    19021 gaaactatct gaatagacaa aaaatccgcc tttgagcgga ttttttatat tcattcttgt
    19081 tagaacaagc cgatagctgt cccgtctgct gccacatcca tgttgagagc tgcgggagct
    19141 tttggcaaac caggcatggt catgacatct ccagtcaagg caacgataaa gcctgcaccc
    19201 aattttggca caaattcacg gactgtaatg tcaaagtctg tcggcgctcc aagggcaaac
    19261 tgatcatctg agaagctgta ctgggtcttg gccatgcaga ctggcaactt gtcccaacca
    19321 ttcttagcaa attcagtcag ctggttacga gctttcttct cgaaaaccac acctgagcca
    19381 ccataaatct gcgtaacaat cttggtcact ttctcttcca agctatcctc agccttgtaa
    19441 agacgttggt agtttgctgc ttgattttca atagtagcaa caactgtctc agccaattca
    19501 acaccaccat cagcgccatt tgcccaaaca cttgccaact cgacaggtac gccaatttca
    19561 gcgcaaagtt ccttcaacgc ggcaatttcg tcagctgtgt cagatacaaa ttcattgata
    19621 gcaacgactg ctggaatacc gtatttctga atattttcaa cgtgacgctt caagttagaa
    19681 aaaccagctt tcactgcctc tacattttca gcagaaagtt ctgtcttggc tacaccaccg
    19741 tgcattttaa gcgcacggtg ggtagcaaca atgactactg catccggaga cttaggcaag
    19801 ttcggcacct tgatatctag gaatttctct gctccaaggt ctgcaccaaa acctgcttct
    19861 gttacggtat agtcggccaa acgcagagct gttgtagttg caagaactga gttacagcca
    19921 tgagcaatat tagcaaatgg acctccgtgg acaaaggctg gcgtgccata gatggtttgg
    19981 acaaggtttg gtttaatagc gtccttcaaa ataagagtca aagcaccttc caccgccaaa
    20041 tcacgcacgt agacaggact gcgatcgaaa cgataaccga taacaatatt tgccaaacgt
    20101 tctttcaagt cattgatgtc tgtcgccaag cataaaatcg ccatgatttc agaagctacg
    20161 gtaatatcga atccatcttc acgcggaata ccattgagcg gaccacccaa tccaacagtt
    20221 actttacgca aagcacggtc attaaggtcc acgacccgtt tccagatgat cctgcgttgg
    20281 tcaatgccta cagcatttcc ttgatggata tggttgtcaa tcaaggctga aagagcgttg
    20341 ttagctgtgg taatggcatg catatcgcct gtaaaatgca ggttgatatc ttccatgggc
    20401 aagacttgtg caaaaccacc accagcagct ccgcccttga ttcccataac aggccccaaa
    20461 gaaggctcac ggagggcaat catagttttc tttccaattt tagacaaagc atccgccaga
    20521 ccaatggtta ctgttgactt accttcacca gcaggcgttg ggttgatagc tgtcaccaag
    20581 atcaatttcc ctggcgcatt gtctttcacc gcattgattt tatcaaagga caattttgcc
    20641 ttgtattttc catagagttc aatatcgtca aagctgatac caactttttc aacgatttct
    20701 gtgattggtt tcaaggttac actttgtgca atttcaatat ctgttttcat gagagctcct
    20761 aatttcctta ttttttatgg acattatacc ataccacagc ttattttcgc atatttttta
    20821 ctatttatgc actaacgtac ggaattttca gaaaaactac aggtttcttt acttaaacag
    20881 atacaaattt ttaaaacctg cattctcatc atagctctac acttccatct aagactgggt
    20941 ttagttagtt ttttcaaaag agagtcagta ttcttttaaa aattgccttg atgagcaagc
    21001 aaacgaccgc ttgcataaag cacttcactt atatagtctt ttagtttact tttagtacga
    21061 ggcaatgagc cgcaggcata actgaagtta ggcaaggcga gttaacgaag taataaaaga
    21121 aaaactaaat gacgataata caggccctta tatttttttg tattagcagc atcattttcc
    21181 agtgtcacag attgattagc aaatcgatta gtgcaaactt tacgaaaaat agaaattcta
    21241 gtaaaatatg aacatgaaac tattgattac atcaggcgga acaagtgaag ctatcgatca
    21301 agtccgcgct attacaaacc atgcttctgg aaatcttgga aaaatcattg ccgaacaagc
    21361 cttgaaacgc ggtcatgaag ttactcttgt gactacaaga caggctgtta aaccagattc
    21421 tcagaagaat ttaaccatta tcgaaatcac aaatgttgat agtttgaaag aaacattaga
    21481 acctttagtc aaatcacatc aggccctgat tcatagcatg gctgtatccg attatacacc
    21541 tgtttatatg actggtttag atgaactaag agcaactgaa gacagttcta gtctactaaa
    21601 aaaacagaat acagaaagta agatttcatc caaggatgac taccaagttc ttttcttgaa
    21661 aaaaactccc aaggtcattt cctatgtaaa aaaatggaat ccagctatca cactctttgg
    21721 cttcaaacta ttggttaatg ttcctaagga ggaattattt gccgtagctc gccaaagcat
    21781 tgaacgaaat ggcgctgatt atatacttgc taatgacttg aaggatattg gagaaaatca
    21841 acacattgcc tatttggttg ataaaacaag ggagattcaa gcacagacaa aagatgaaat
    21901 tgctcagctc attttaaata ccctagaaaa aggagaacaa aatggctaat atcacacttg
    21961 ccgttactgg ctctatttca gcctacaaag cagctgacct aactagtcaa ttgaccaagc
    22021 tcggtcatca ggttacagtc ctcatgagtc gctctgctat ggacttcatc acacctttga
    22081 ctctccaatc cttgtcaaaa aatctggttc acacagatgt catgattgaa gaaaatccta
    22141 ctcttatcaa gcatatcgat attgccaaag aaacagacgt atttttagtt gcccctgctt
    22201 ctgccaatac gatagctaaa ttagctaatg gtatggctga caacattatt acggcaacag
    22261 ctctggctct gccaattggt actaaaaaac tgctggcacc tgctatgaat actaacatgt
    22321 acctcaatcc tgctacccag aaaaatatca aaaccctcat agagtatggt tttgaggaaa
    22381 tcaaaccgcg tgaagccctg cttgcttgtg gagattttgg aactggggcc ttggctgagg
    22441 taactgatat tttagagaaa gtgagtaaca cccttgatga aaaaaaataa atcaactcaa
    22501 attgctacaa ttgcaatctt ctttgctgtc atgattgtca tcaatatatt gagttcaatt
    22561 atttttaata tattgcctgt tccaataaaa cccaccatcg tacatatccc agtcattatt
    22621 gcttctatta tatacgggcc acgtgttggg gcttgtttgg gggctctcat gggcttaatg
    22681 agtatgaccc ataatactat tatcttacta ccaaccagct atcttttctc cccatttgtt
    22741 gaaaatggta atgtttattc aatcatcatt gcagttgtcc cacgtatatt agtcgggatt
    22801 acaccttatt tcgtctacaa atggataaaa aatcgtaaag gattatttat tgctggtgca
    22861 attggttcaa tgaccaatac tatttttgta cttggaggta ttttcttcct cttctcctct
    22921 gtctttgatg gtaatattca agcaatgtta gcaaccgttt taggtaccaa ttcaattgca
    22981 gaaatgatca tttcttctgt attaactgca gcgcttgttc caactttaca gaaaattcaa
    23041 aaataaatat cagaaaatca tgtgaaagcg tgatttttct tttcaaacat ggtataatga
    23101 aagcgtttaa tagaaaaatt tttaaggaga tatgcgatga cttatcaaga aacctatcaa
    23161 acatggctcg actttgcgga ccttccagat tacttgcgtg aagaattagt cgcaatggat
    23221 gaaaaaacaa aagaagatgc cttctataca aatcttgaat ttggtacagc tggtatgcgt
    23281 ggctatattg gcgctggtac caaccgcatc aacgtattcg tagtccgtca agctactgaa
    23341 ggtttggcaa aattggtaga atcaaaaggt gaagaagcta aaaaacgtgg tgttgccatc
    23401 gcttacgatt cacgtcactt ctcgccagaa tttgcttttg aatcagctca agttttggca
    23461 gcgcatggta tcaaatctta tgtatttgaa agccttcgtc caactcctga gttgtcattc
    23521 gctgtgcgcc attacaatgc catcgcaggg attatggtga ctgcaagcca caacccgaaa
    23581 gaattcaacg gctacaaggt ttacggtgaa gacggcggac aaatgccacc tgctgatgca
    23641 gatgcattga ccaactttat ccgtgccatc gacaatccat ttgcagttga attggctgac
    23701 cttgaagcca gcaaggaaaa tggtttgatt acggttcttg gcgaagaaac tgacgttaaa
    23761 tatcttgaag aactcaatga cctcaacatc aaccctgaat tgattgctga atacggtaaa
    23821 gacatgaaga ttgtctatac accgcttcat ggtacgggtg aaatgttggc gcgtcgtgcc
    23881 cttgcacagg ctggttttga atctgtccaa gttgttgaag cacaagcaac tgctgatcct
    23941 gacttctcta ctgtcgcttc accaaaccca gagagccaag cagcctttgc ccttgcagaa
    24001 gaactcggcc gtgaagtcgg ggcagatgtc cttcttgcaa ctgaccctga cgctgaccgt
    24061 gttggtgttg aagttcgtca agctgacggt tcttactgga acctgtctgg taaccaaatc
    24121 ggcgctatca ttgctaagta catccttgaa gcccacaagc aagcaggtac acttcctgca
    24181 aacgctgccc ttgctaagtc aatcgtatct actgagttgg taactaagat tgctgaaagc
    24241 tatggcgcta ccatgttcaa cgtcttgact ggtttcaaat tcatcgctga gaaaatccaa
    24301 gagttcgaag aaaaacacaa ccatacttac atgtttggtt tcgaagaaag ctttggctat
    24361 ttgattaaac catttgtacg tgacaaggat gctatccagg ctgtgcttat ggttgctgaa
    24421 attgctgcct actaccgttc acgtggcatg accttagctg acggtatcga tgaaatcttc
    24481 aaagaatacg gctactttgc tgagaaaaca atctctgtta ccctttctgg taaggatggt
    24541 gcagaacaaa tcaaggcaat catggctaag ttccgtgata actcaccagc acaattcaac
    24601 gctactgaca tcgcggtctt tgaagacttt gcccttcaaa ctaagacaga caaagatgga
    24661 aacattgaaa aactcactac tcctccatca gatgtcttga aatacacctt ggcagatgat
    24721 tcttggttcg ctgttcgtcc ttcgggaaca gaaccaaaaa tcaaattcta catcgcaacc
    24781 gttggtgaaa cacttgctga agcagaagaa aaaatctcca acatcgaaaa agaaatcaac
    24841 gaatttgttg ggtaagagtc acataaatta gaaagtacaa accacccgtg aaaacgggta
    24901 gtttgtacac tggctataag cccacatcac cagccagcgc ctaaagacgc tggctttcac
    24961 tttgttcaag cctcactgct tttgactcgt cactagcctc ttaaagaggc attcgtacta
    25021 cttgccatta tccctaaagg gatcttcata ctcttttaca ctcaatttat caagtgctat
    25081 atcatgttta actcgctttc tagatttaag ctcgtgaaga aatcatccac tggataattt
    25141 ttttgttctt gtatatattt cttaattgtg gcttcattaa gtccaactta cactcttcaa
    25201 caattcccca aattgttgaa gccctctgtc cagaaatgac gattgccaaa tttatatttg
    25261 agattagcgt gtttatcaaa catcataaga gcactcttgc cttttaaata tcccataaag
    25321 cttgatacac ttaatcgcgg aggaatactg actaacatat gaacatgatc cggcatcaga
    25381 tgtccttcga taatttctac gcctttataa ctacacaaat gtcggaatat ttctcccaaa
    25441 ctacttcgat attgattata gatgattttt cgtctatact taggtgtgaa gacaatgtgg
    25501 tagaacacaa ccactttgta tgtgataaac tatgtgcctt ttgtgccata cttttctcct
    25561 ctcgctttac aattggcttg aacaccttta ttgtatcgcg tttagagttt tttggtataa
    25621 ccttcgtcgc gcacccgcat agcgggtggt ttatttgtca cgcaccttac ggagcgcgac
    25681 ggactaaaag tcacataaat acaataagca acgggggaac ctcgttgctt atttctatct
    25741 agaatactac cattctagtc tcaaactatt aaaggattat ctatagcagg ttatagcaac
    25801 tataggaact gattcactta aaaaactaat tcgttgaaaa tatagtcttt tagtttactt
    25861 ttagtactag gcaacgagcc gcaggcataa ctggagttag gcaaggcgag ttaacgaagt
    25921 aataaaagaa aaactaaatg acgatacttc ttttacttac gtcttttctt taacaaacct
    25981 aaagcactac caagaacaag agtcaataga ccgactccca taatctgtct tgaagtaggt
    26041 gtacttactt tcgaagattg ttctatgcct aaaatttgtt ttgcacctgc ttgagattga
    26101 actcccatat tcttaccaga ttcaatagta ttatttgagg tttgtgagat attttctacg
    26161 agcactacag gctcagaagc agatgaaacc gtttcaacta actctgtctc cttgtgttct
    26221 ataacaattt ctgctttaga cacctgacgc tctgtattat tagactctaa taacacagta
    26281 ttctgatagc tcatacctat ctcagtacta tttgagtttg aagactgagc aatggtggct
    26341 tctagcaatg gagaccctgc ttctactaag cttgattcta gcaattcttt cttaataact
    26401 tttgattgaa cagagattag cccttcagta tttggttttc taacctctct tttcgataat
    26461 gaagaaactt ccacttctgc attcgtgtcc ctcttagaac cgataagggg gatagctact
    26521 ggttcaatca ctggattatc cagattgact gatgaaacag cagttagaga ggtatctgca
    26581 tgaatttcag aattgtcgac agatgcagct tcgacattag ttgatttgct cgaggttggt
    26641 tctgcaattt ctgtctctcc agcctgaatg gagtcttttt caagcagagt agctacagat
    26701 tcagttgtgg aactatctgc atatgccgag gaaccacggg gagtaactga agaggtagct
    26761 gaatggattt cagaattgtc gacaagttca gcttcaacac tagttgattc gctcgaggtt
    26821 ggttctgcaa tttctgtctc tccggcttga atggagtctt tttcaagtgg aatagctaca
    26881 gattcagtta cggaattatc tatatacatc gggaaaccat gaggagtagc tgcatcatta
    26941 ataggagtga ccacagattc agtttcgaca ttcattggtt ctacaggaga gatttctaca
    27001 ttttgatgtg gtattgagtc tggttgttca gaaacaaccg cctccactct atcttcttct
    27061 ttgtcatttg caccctctac gattagggtg tctgacagaa gttccgaact ctgattttca
    27121 actttttctg attcaagaag cttttcaact tggtctacct catcattttt cacatcttct
    27181 tcaacagcta ctactagcga atccgtttct gacgtttgca tgtttcctga tagttctgaa
    27241 ccaaattcat ccttgttttc aataattatt ggtaatgtat ctgcctcttg ttcaaaaaca
    27301 ttatctccct cagcctctac tgaagatgtc gtttgggcct ccactggaaa atgcaattga
    27361 ttggtaccat ctgcaataat acctgcattc tctgtgtctg cttccaaatt tgtggaactt
    27421 gtttcttgag atgatagaga atcaacatct gaaaccgttg agcgatcacc tactgataaa
    27481 tctggtgcca aagcatttcc aacttctgaa ataggaaaat ctaaactctt taaactattt
    27541 aaaccatttt ccaatgcacc acttagcatc tcagaacttt ctgtcttttg aatagataga
    27601 atcccttgat tatagagatt atttagtttt tcaagataaa tcagccgcat tgattctggc
    27661 aattcagatt gtccttgaat agacagaacc aacgccttct ttctatcagc tagtatctga
    27721 ctagctattt ctttggcttt tgcgaacgga ttgaaatatt cttcccataa gtcttgacca
    27781 agacggattg tatggacata atccaaaagc gccccagctg aattattttt cacatgatta
    27841 ttcaaacgcg gatttccaga agcatcacga gtgattccca tacgcttcaa accaatttgt
    27901 tcttgttgat catcggaatc agtgatatag acagccctaa tcttaccttg attatcgtac
    27961 tcagcacccc ataccgtcac aatatgcgtt gcatagccta atgttctata agttagaccg
    28021 agtaatcctt tgctctccaa aacagttcga acatcctcac caaaacgctc atagctacca
    28081 ctaaaaatac gatttgtcag ttttttctct ttgaaaacgt cgtagaaaaa gccaccccta
    28141 ctatctggta ctaactggct atcttccaga ttgacaccac cctgtacctt aggtttatat
    28201 ccattaataa acaagtcaac tagggcatct gacacaaaac catttgtacg ataaccgtag
    28261 taagttttga acatatcaaa gactcgacta ttttgctgat cctggaacga atcaatatag
    28321 cgacgtaggt ccgttattgc atagttttcc ccaacattta ctgtaccctt tttttctttg
    28381 agatatcgct caacatagga actattctgt tccaaccacc aatgtaccat gttggaagac
    28441 acggctgcaa aacataggtt aaggtcaata aatgaagcat tgagactttt attggtatca
    28501 taatagcctt ttcctgcctt aaatggcgct gtatagttta aatagtctcc atacttttcc
    28561 tttacaaaac caccgctttg ttccattgca ggaggagtaa ttccatgggc ccatataatt
    28621 tgtcgttgct cgccactctc cgtttgataa ctggtctgat tcaaaatttc ttttgtctca
    28681 gtaacctcta tctgattttg ctcgctcgtc gtaacttctg tatcatttct agaaactaca
    28741 ttctcggtcc ctctgtcaac tttcatctct accgctactt ccccttcagc gataacaggt
    28801 gatgggatgt tgtctgctat ttcctctaca atttcaatat tagaaccatc tagggattca
    28861 tttttttctg attcaataga tacctcatcc ttgacttgtg attcttcaat tatttcattc
    28921 actcctgtaa caactgtatc ggcagcaaca gtggatgtat aaatagcaca tagcaaagag
    28981 actgagacca agccaaccgc ggattttctc aaagaaaatc gttcttgaat gttcatcttt
    29041 cctccgttta tatttatatt ctttgaacaa gttattctta ctcttattta ttttttatat
    29101 caacatccca aaatcattta gagacaaaaa atctataaca gcacgtattt aacttattct
    29161 ctaattacta caaaaaggtc caagcggacc tttttattat tttactgtac gaacacgcat
    29221 agtgtttgtt gaacctgcaa cacctacagg cacaccagca acgataataa tgtcatctcc
    29281 tgcttcaaca agaccttgct ctttggcaat acgttcagcc aaatcaaaca tatcgtcagt
    29341 tgaatctgga cggtttgtta caattggaat aacaccccag ttaagcatca atgatttttg
    29401 tactttctca gtaaatgtta ctgccaagat gtccgcatct ggacggtatt tagagatcga
    29461 acgagctgag taacctgttt ctgtaactgt tacaactagt ttgatgtcca ttgagtgaca
    29521 tgcgtctttt actgcagaag caataacttc tgttttagag gcacgattaa actgatcaga
    29581 gttcaaacga ccatattcat taagaagagt ctgtgcgttt ttagcgatag ttgccattgt
    29641 acgaactgat tctactggat atttaccgtt tgcagactca cccgaaagca ttgttgcgtc
    29701 agtaccgtcg ataactgcgt tgaatacgtc agatacttca gaacgagttg cacgtggttt
    29761 ttcagtcatt gtttccaaca tgtttgttgc tgtgataact gctttaccag ctgcattcac
    29821 tttagtgata atcatttttt ggtaaactgg aaccatttcg aatggtactt cgatacccat
    29881 gtcaccacga gcaatcatga taccatctgc tgcttcgatg atttcgtcga tgttgtcaat
    29941 accttgttgg ttttcgattt tcgcaaacaa ttgaacgtgg tcgttaccag tttctttaca
    30001 gatgttacga acaacttcta cgtcttttgc agtacgtaca aatgagatag caataaagtt
    30061 caaaccttgc tcaagaccga agcggatatc tgcattatca cgctctgcaa gagctgggaa
    30121 aggaattttt gtgtaaggga tgtttacacc tttttgttta gcaattacac cgtcgttttc
    30181 aaccaaaaca acaaattcac ggtttactgc atctttttca acaactgtca aaccaagttt
    30241 accatcgtca acaaggattt gcttaccaac ttcaacatcg tcaaagacat ccaaaccacc
    30301 tgctacgttc aaggcaatca cttcttttgt agatttgata ccttgttttg tagcaacacg
    30361 caatttgtca ccagttgtat atgcatattc ttttgcatca tcttcaaaca attctgtacg
    30421 gatttctgga ccttttgtat ccaacaagaa acctactttt ttaccagcga tttcttctgc
    30481 acgacgaact gttgccatac gatcaccttg ctcttggtgg tcaccgtgtg agaagttgaa
    30541 acggaaaaca ttggcacctt ctgtaatcaa agcagcaatt ttctgtgctg atgactcaac
    30601 atctaatttt tcaccccagt aaccatcgtc accgaatttt ttaccaccgc ggatttctac
    30661 cgctggacca agggttgcaa caattttaac acgtttgttc atcttaacaa aactcctttt
    30721 atttcaatgg tcatacgacc cttaattaac acttttaaac aacgatacct agattgacaa
    30781 gttacggttc aaatcagcca actccaaatc tgctttatgc ggattattaa ctacaatctt
    30841 gccatcagta gtcaagctaa acaaggcacc ttcttctgca gttccaagaa ttggattttc
    30901 aaccatttgc tcgttacgga tacctactgc aagacctcca cggccttctt taagcaattt
    30961 aacggcatga gcacccatac gagaagcgag tacacgatca cgcgctgttg gagaaccacc
    31021 acgttggata tgaccaagtt ctgtcacacg aagatcactt gtatcgcctg ctgctttcaa
    31081 ttcttcagcg aactgtgcag ctggcataac accttcagcc aaaacaatga tgctatgttt
    31141 cttaccattg tcgtagcccg ctttaatacg gtcaacaata tcgttgatat tgaagtcttc
    31201 ttctggaaca acgataacat ctgcaccaga agcaatacca gcccaaagag cgatgtctcc
    31261 tgcatgacgt cccattactt caatgacgaa agtacgacgg tgacttgatg aagtatcgcg
    31321 aatcttatca atcgcatcca tggcagttgt tacagctgtg tcaaaaccga ttgtaaaatc
    31381 tgtccccacg atgtcgttgt caatggtacc aggtacacca atagctggga aaccatgctc
    31441 cgtcaagcgc attgctccgt ggtaagaacc gtcaccaccg ataaccacta caccttcgat
    31501 accatgtttt ttcaattgct caatcccttt aagttgccct tctaatttgg caaattctgg
    31561 ataacgagct gaaccaagga atgttcctcc acgtgaaata atatcaccta cagaacgtgc
    31621 agacaattca tggatatcac ccgcaaccat acctgcgtaa ccttcgttga ttccataaac
    31681 ttccattcct tctgaaattg cttggcgaac aactgcacga atagcagcgt tcataccagg
    31741 ggcatcacca ccactggtca aaacagcaat acgtttcatt cgttaatgct cctttaactt
    31801 ttcattttaa ctgccctatt ataacatact tagcctaaac ttgcttgact ttttgctatt
    31861 tttttatcga aaaatcgttt tcataacaaa ttcacttaac tcttcttcta attgaggcgt
    31921 tttcataacc atgtggtgct cagcctgaat tgtctggtta ctatcttgat aatgaagtac
    31981 tactggaatg cttcctctat atttatccaa aatacgagca acagcataat cgtgttctcg
    32041 attttccaaa agaatccaaa atttctccaa actgacaggc tctaattttt ctagaactaa
    32101 ttgcaactgt ccatcgcgct cttgcactct acctgtgatg taccccattc ccccttcttc
    32161 caaaatagaa gcgtattggc gataagattc tgggaaaaga gtcacgtcaa gcgtcctctt
    32221 ggtatctgtc actcgaagaa atgccatctg ctccccagtc tttttggtac gaatcaatcg
    32281 aatagcttga atctgcacaa gaacggtcgc tcgattgcca accgctaaat cagccaaatc
    32341 tgtaaaacta cgcttagact ctcgacaaat attgactaaa ggatgcggac taataccaac
    32401 acctagcaat tcttgctcta gcataaattt ttcactgtcg ctgtaatctt ctacatctgt
    32461 ccaactataa ctctcctctg caaaaaatgt accaatcgcc tctgcaaaga caaagagatt
    32521 gtctagattt tcaataatct ttttacgatt ggcgacaaaa tcatcaaata aaccaatctg
    32581 aataagaggg attagtaaat cttttttctt gaaattatct ggcaagcgca aaataaaatc
    32641 ttctacagac ttatagggac gatgatccaa tatccataaa gctaaatctt taggtaaacc
    32701 ttttagtgtt tttaatccta ggtagacctt acttttatca aacttatcgt gataaggaat
    32761 attgttaatc gtgagtcgag ctacctcaaa gtcaaactgc atcgcatctt caatataagc
    32821 accgctagaa tgattgagca tgacatcaaa aaagacatcc gtatagtgac tcttgaaata
    32881 ggccatttga aaggctaagg ctgaataagc ataggcgtgg ctgcggttaa aaccataacc
    32941 agcgaactta gccaccatgc caaatatttc ccttgcattc tcttcagcat gtccgttttt
    33001 cagtgctccc tgtaaaaatt ccgcttccat tacctgcatt tcctgggcat tctttttaga
    33061 catcgccctg cggagtaaat ccgcctttcc aagcgtaaat cctgcataga cctgtgcaat
    33121 ctgcatgacc tgttcctgat aaagcatgat accataagtc ggttgtaaaa taggagcaat
    33181 ggttgggtcc aacaaatcaa ctgtctcttg tccatgtttg cgttttacaa agttatccga
    33241 ataatcacta gcacccggtc tattcaaact cgttgttgca actatatcct caaatcgacc
    33301 aggtttgacc cttcgaagca ggctaatcgc accaggctgt tcaaattgaa aaattccctt
    33361 agtccgacca gcagcaaaaa gttccaaggt tttcttatct tctaaatcta tcttggcaat
    33421 atcaatttcc tgctgataac gttcacgtac tccttctgcc attttttgca caaaggtcag
    33481 atttcgtaag ccaaggaaat ccatcttcaa cagaccattc gcctcaacag catgtgcatc
    33541 atattgggtt accagcatgt catcccctat tttaagaggg atggtatctg tcaaatcctc
    33601 atcactcatc actatccctg ctgcatggat agaagtctgt cggggttgcc cttcaatttg
    33661 ttgagcaatc gcaaaagctt tttggtactc tatcttacta ttgataatct gacgaaaagc
    33721 ggcatttcgc tgataggcag aagtaagact atccctaaat gaaattttct tggtaatatt
    33781 acttaactca tattcaggtg ttccgaaacg cttaaataca tcacgaatgg cctgcttagc
    33841 tccaaatgtc gagtaggtta caatttgcgc agcatgggtt gaaccataac gatctcgtac
    33901 ataacgaata aaatctgctc gatgaatatc tggaatatca atatcaatat cgggcattga
    33961 ataacgttca gcattgagaa agcgttcaaa gagaagatta tgcttaacag gatcaatacc
    34021 tgtgatattt aaggcgtagg caactaggct tccaacagct gaaccgcgcc ccattcccat
    34081 gtagtaccct tggctacgcc caaaacgaag taggtcccat acaatcaaga aataatcgtc
    34141 aaatcccatt tgatgaataa tggataattc cttttccaat cgttccttat acagtgaatt
    34201 agtcaagccc ttctcttcta atccttgata ggagcgctcg tataattctt ccactgcagg
    34261 tctgtcacga ttaaaacgcg gcaattttaa atctgtattt aattgataag aaatccctgc
    34321 aaccaagtca cgtaaattat caactgcttg cggaaaccga ttcgaaaata gattggttaa
    34381 actatcagcc gataaaagct tttgtcgact atctatatct acaacttgat taagtggaat
    34441 attatctcga atagcatgta gaacctgtag actttccaat tgttcctctt caaaaaaacg
    34501 aaccgtatga attgggagta ttgcttgctt aaagtcttga actggtgtat caaccgtcac
    34561 tccgatataa tagttaacac ctaactcaag gtgctcaatg ccagaaaaat aaggaacaac
    34621 cactgctatg tcgactaaat attgacgaat agtttcaaaa tcatcttgcc ctatcatctt
    34681 agcagtcgaa attttcaata gattgcgata acctctggtt gtcagagcaa tcaattgagt
    34741 agttacgtcc tgctcccctt cctgtaattt ccagtccagt tcacaaccaa taattggttg
    34801 aagccccatt ttgttacagg attccataaa actataggct gcataaagac tgtttttatc
    34861 cattatcccc aatgcgtgat aaccgagctc tttagccctg cctacataat tttctatcgt
    34921 caccatgcta tccataaagg tataaactgt ctttgtatct aattgtgcta gcatggcatt
    34981 ctccctttgt tgcttcttct taaattatat caaataatca tcaaaaaata ttttaaaaaa
    35041 a
//
LOCUS       DBNFEG010000005        32584 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.005, whole genome shotgun sequence.
ACCESSION   DBNFEG010000005 DBNFEG010000000
VERSION     DBNFEG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 32584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32584
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.005"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(76..819)
                     /locus_tag="AC1PNG_000129"
     CDS             complement(76..819)
                     /locus_tag="AC1PNG_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HIP6700672.1"
                     /translation="MKTRIVQKQFKRKETKYIVDKKTFELLEKELRQYMMADEFATST
                     ITNIYFDNEDFDMIQDSLAKKNGREKIRMRVYDAIPSESSQAFLEIKKKENKIGYKYR
                     LTSNPVSVANYIENGVIDSTIKDDKVNSELEMLRERYGTIKPKMYIYYDRVSYKGIED
                     KKVRLTIDKNLLYRDYDVDAMEGKFGKNLLDPTKVIMEVKVPEERPDWLVALLEKYQI
                     EKQSFSKYGNAYKLAHNITGEEVSQHAVV"
     gene            1012..1314
                     /locus_tag="AC1PNG_000130"
     CDS             1012..1314
                     /locus_tag="AC1PNG_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700673.1"
                     /translation="MYEFVLEYGSFPVKLIDGFVNNRSEIPDFLKEDEEMIARLNEIN
                     ELFHQLFLTIECKFDYIGKQFPDKIEQLRELYHPLADDLLAKYGNQIELKIEPFIL"
     gene            complement(1335..1601)
                     /locus_tag="AC1PNG_000131"
     CDS             complement(1335..1601)
                     /locus_tag="AC1PNG_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HIP6700674.1"
                     /translation="MNTQDIQEKFFREGKLIVIPKKLKSKQVLFAYLQEELAKKGSAF
                     TEKEVNAFLAEIYDDYAILRRYLVDYGYLSRDQYGLEYRIEEKR"
     gene            complement(1656..4055)
                     /gene="pheT"
                     /locus_tag="AC1PNG_000132"
     CDS             complement(1656..4055)
                     /gene="pheT"
                     /locus_tag="AC1PNG_000132"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIP6700675.1"
                     /translation="MLVSYKWLKELVDFDATSHDLSEKMSTTGIEVEGVEQKSAGLSK
                     LVVGQVVSAEPIPDTHLNTCQVNVGDEITQIVCGAPNVKPGIKVIVALPGARIAGNYK
                     IKKGKIRGVESLGMLCSLSEIGVSDSVVPKVYADGIYHLPEDAVVGEPVFSYLDLDDE
                     IIELSITPNRADALSMRGVAHEVAAIYDSKVNFKPVVLEESDKKASDVIEVAIESDKV
                     AAYTARVIENVTIAPSPQWLQNLLMNAGIRPLNNVVDITNYILLYFGQPMHAFDFDKF
                     DGKKIVARQAKEGENLVTLDGEERDLITDDIVISVGDKAVALGGVMGGANTEIDNNSK
                     TVVLEAALFDGKSIRKTSGRLNLRSESSSRFEKGINVATLTEAMDTAAAMIAELAGGQ
                     VLSGIVSAGSVDTSDVPVTATIDYVNRSLGTNLDYAQIADIFRRLGMETTGDASQFTV
                     AVPRRRWDIRIPADLVEEIARIYGYNNLPTTLPKEDGTAGELTLTQNIRRQVRSLAEG
                     AGLTEIISYALTTPEKAVEFAARPTTVTELMWPMTVDRSALRQNMVSGMLDTVAYNVA
                     RKNKNLALYEIGKIFEQSGNPKEDLPQEINKFALVLTGLVAEKDFQTPAVPVDFFHAK
                     GILEAIFGHYKLAVDFVATSEIAALHPGRTAEIHLNGAVIGFVGQVHPQTAKDYGIPE
                     TYVAEINLDAIEEALQPAQPFTEISKFPAVSRDIALLLSSDVKHQDVLDAIAGAGVKR
                     LTKVTLFDVYAGSNIEAGKKSMAYNLTFQNPADSLTDEEVAKYMEKISKALVELGAEI
                     R"
     gene            complement(4057..4578)
                     /locus_tag="AC1PNG_000133"
     CDS             complement(4057..4578)
                     /locus_tag="AC1PNG_000133"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6700676.1"
                     /translation="MQIDPICLEEVSVLQELAKQTFAETFAHDNDPKELEAFFEESYS
                     LEVLTSELSDPNCQHYFLRIDHQIAGYLKLNQGPAQTEQELENAFEIQRIYLLQAFQG
                     RGLGKVLFEFALEKAEQSSCDWVWLGVWEHNYKAQKFYSKYGFEKFGQHEFAVGDKID
                     VDWLLKRPLHQKG"
     gene            complement(5107..6150)
                     /gene="pheS"
                     /locus_tag="AC1PNG_000134"
     CDS             complement(5107..6150)
                     /gene="pheS"
                     /locus_tag="AC1PNG_000134"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIP6700677.1"
                     /translation="MSNIEQQLAELSQTTLEKLKEIQHQGEKELQDLRVAVLGKKGSL
                     TDLLKGLKDLSNDMKPIVGKQVNEVRDVLTTAFEETAQKVAAAKIQQQLASETIDVTL
                     PGRQVKVGKRHVLTQTSEEIEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDM
                     QDTFYITEEILMRTHTSPVQARTMDQHDFSKGALKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGENVSMGDLKGTLEMIIKKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     DGCNVCKKTGWIEILGAGMVHPQVLEMSGIDSTKYSGFAFGLGQERIAMLRYGINDIR
                     GFYQGDVRFSEQF"
     gene            complement(6605..8329)
                     /locus_tag="AC1PNG_000135"
     CDS             complement(6605..8329)
                     /locus_tag="AC1PNG_000135"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIP6700678.1"
                     /translation="MPRLVDLRKKPYCLNDEQITWVEDTLAHLTDEEKIGQLFVNLFF
                     FGGDAFSGNNLSNQELMNRYHIGGARYMNGSPEQVQGLINELQSYSKIPLLVAANCDS
                     GGDGAVKGGTYISSGAQSEASRDPRIPYLAGLVSAREETALGVNVNFDPCVDIVQNWR
                     NTIVNTRAYGTNADDVIAYTSAYLKGLTAERDVIQCIKHFPGDGTEERDQHLVLGVNE
                     LSPEEWDKSFGRVYRHHIEAGVEMIMAGHIALPYYQQRLNPTLKDEDILPATLAPELI
                     QDLLKEKLEFNGLVITDASHMLGMTAAMRREDYVPAAIAAGCDMFLFFNNLEEDFEFM
                     LNGYRKGVITEERLHDALRRILGLKAKLNLHIKQAEGTLLKSADELAIIGCEEHLAWQ
                     REAADKAITLLKDTQKNLPISPETHRRIRLYYLEGEKEGIMAASTEVVDNLKAALEAR
                     GYEVTVNDGTTRIKGKTLQYREEVDLALTVANVIGYGAQNNYRIQWKTAMSNEVPWYV
                     HEVPTIFVSLNYTTHLHDVTMVKTAINAYHHNINTIESLLDKLEGKDDFYGVPNENVW
                     ANKWQAKL"
     gene            complement(8340..10130)
                     /locus_tag="AC1PNG_000136"
     CDS             complement(8340..10130)
                     /locus_tag="AC1PNG_000136"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIP6700679.1"
                     /translation="MTHLVDLRKKPYSLDEEAISWIEETIAQMTLDEKIGHLFVNMGS
                     QRTEEYLTGVLNDYKIAAVRYNPGPAADIWEQNYILQTKSKIPLLIAANTESGGNGAV
                     TDGTKIGDEIKIAATADPRYAYEMGKVAGLEAAAVGCNASFAPIMDLSRNWRNPIIAN
                     RTWGANVDQVIELSKEYMRGIMEHGIVPFAKHFPGDGIDERDHHLSFASNPMTKSEWM
                     ETFGRIYGEMADAGLPGIMAGHIHLPNVEKEMHPERELDDMLPASLNKTLLDELLRGE
                     LGYNGAIVTDASHMIGMTASMSRRELLPTAIEAGCDLFLFFNDPEEDLQWMKEGLENG
                     LLSEERLHDALRRTLGLKAKLGLHLFEGRRQEIMMPKEEALALIGRDEAKQLAKEVAD
                     KAITLVKAKQEGIFPVNPERYKRILLVEVDGYKGGFGAMINAGKKRAADTLKELLEAR
                     GHQVSIWENTEARIAKLPEEERPAAIANVYASKRPIAEITDNYDLIINLVDVNSGGTT
                     QRIIWPAAKGTPDQPFYVHEIPTIVVSVQHPFALADMPQVATYINAYDGLPVTLEALV
                     AKLAGESDFTGVSPVDAYCGLVDTQIWRGK"
     gene            complement(10180..10980)
                     /locus_tag="AC1PNG_000137"
     CDS             complement(10180..10980)
                     /locus_tag="AC1PNG_000137"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIP6700680.1"
                     /translation="MMKTDYVFCVDSDGCAMDTMTYKHKLFFGPLAADVFDVKDREPF
                     LAEWNRVNLYSRERGINRFVGLVKGLDFAGVTGIDNLKNWVATTDSLSNASLERLIEE
                     TPSKDLELALEWSTQVNQAIKHYSGPVLAFIGVHKGLEKLSQLGKVYVVSSANKEAVE
                     EEWTDQGLLDFVTELYCQDRGKKEDVIKILIEEGYCPDKIMMIGDSPGDLKAAELNGV
                     HFYPILVGQEMQSWADLTETIADEFAHQAFTGEKETELIQAFWNNLDD"
     gene            complement(10980..11819)
                     /locus_tag="AC1PNG_000138"
     CDS             complement(10980..11819)
                     /locus_tag="AC1PNG_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIP6700681.1"
                     /translation="MTRVIEFKDKVVVITGAGGVLCGYLAKEFAKAGAKVALLDLNEA
                     AAQVFVDEIAAAGGVAKAYKSNVLSKENLEGVRQQVLADFGPVDILINGAGGNNPKAT
                     TDNEFHEIGLPADTKTFFDLDEAGINFVFNLNYLGTLLPTQVFAQDMIGRSGANIINI
                     SSMNAFTPLTKIPAYSGAKAAISNFTQWLAVHFSKVGIRCNAIAPGFLVTNQNRGLLF
                     DESGQPTARANKILTNTPMGRFGEAEELVGGVFFLADENLASFVNGVVLPIDGGFSAY
                     SGV"
     gene            complement(11821..12921)
                     /locus_tag="AC1PNG_000139"
     CDS             complement(11821..12921)
                     /locus_tag="AC1PNG_000139"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HIP6700682.1"
                     /translation="MKMSFRWYGKKDPVTLEEIKAIPGMQGIVTAVYDVPVGQAWPLE
                     NILELKKMVEEAGLEITVIESIPVHEDIKQGKPNRDELIENYKTSIRNVGAAGIPVVC
                     YNFMPVFDWTRSDLHHPLPDGSTSLAFLKSDLAGVDPVADDLNLPGWDSSYSKEEMKA
                     IIENYRQNISEEDLWANLEYFIKAILPTAEEAGVKMAIHPDDPPYGIFGLPRIITGQE
                     AVERFLNLYDSEHNGITMCVGSYASDPKNDVLAMTEYALKRNRINFMHTRNVTAGAWG
                     FQETAHLSQAGDIDMNAVVKLLVDYDWTGALRPDHGRRIWGDQTKTPGYGLYDRALGA
                     TYFNGLYEANMRAAGKTPDFGITVKTVGNKES"
     gene            complement(12936..14336)
                     /gene="uxaC"
                     /locus_tag="AC1PNG_000140"
     CDS             complement(12936..14336)
                     /gene="uxaC"
                     /locus_tag="AC1PNG_000140"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HIP6700683.1"
                     /translation="MSFNDKNFMLKNEPAKELYGKIAELPIYDFHCHLDPKEIFEDKV
                     YEDIVDLWLGGDHYKWRLMRANGISEEEITGSASKLDKFKAWARTLERAFGNPLYHWS
                     HLELRQVFGVEELLTEENAERLYHQLNAYLQEHRVSPRKLIADARVTFIGTTDHPLDS
                     LEWHKRLAEDSTIDTVVAPTFRPDEAFIEHRNFNLFMKRLEEVTGVAVRDFASFVEAL
                     GQRVSYFAQHGCRASDISFTAISYEEATLEELDEILLARLAGKEAGQSSINKWQTAIF
                     RELCRLYKKHGFVTQVHFGALRNNHTGLFEKLGADVGVDSIGDQTCLTVNLNRLLDNL
                     VKENALPKMIWYNLNPSYNIALANTLANFQSNEEGLRSQLQFGAGWWFNDTKLGMIDQ
                     MNAYAEQGMLANFVGMLTDSRSFLSYQRHDYFRRILATYVGQWIVDEEVPEDYNRLGQ
                     FVEAISYYNAKEFFEQ"
     gene            complement(14338..14958)
                     /locus_tag="AC1PNG_000141"
     CDS             complement(14338..14958)
                     /locus_tag="AC1PNG_000141"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - (R,S)-4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIP6700684.1"
                     /translation="MLKQLKENYFFAVIRGKDEEDAKEIARHAILGGIRNIEITFSTP
                     NAATVIKELQEEFSYDSSVVIGAGTVMNLKLAQAAIDAGASFLVSPHFDKEIQDLAQE
                     AEVFYFPGCATATEIVTASQAGCPIIKLFPGGVLGPGFIKDIHGPVPQVDLMPSGGVS
                     VENVADWKKAGACAVGVGSALASRVQAEGYESVTTIARSFVAALEG"
     gene            complement(15081..15797)
                     /locus_tag="AC1PNG_000142"
     CDS             complement(15081..15797)
                     /locus_tag="AC1PNG_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIP6700685.1"
                     /translation="MAKPLVEKASERLLELILERGYEVGDKLPNEYELAQDLEVGRST
                     VREAVRSLATRNILEVRQGSGTYISSKKGVSEDPLGFSLVRDRVKLTTDLFEVRYLLE
                     PRIAERVAQFASDEDIARLEEIVLAIEEAVAAGDTKHLELDVKFHCMLAEMSGNLAMT
                     SLMPVINQSIHLINENYSNRQMKSDSLQAHRNILMAIKQRHPVAAYDSMLAHIISVRQ
                     VVLSEWFDKDMTLHGLNDHK"
     gene            complement(15843..17642)
                     /gene="uidA"
                     /locus_tag="AC1PNG_000143"
     CDS             complement(15843..17642)
                     /gene="uidA"
                     /locus_tag="AC1PNG_000143"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HIP6700686.1"
                     /translation="MLYPIQTKTRSVYSLNGIWKFKQGNNDVHTLLDTDEVMVVPSSF
                     NDLVVDKDKRRFVGDNWYELMVALPVVSADEELVVRFGSVTHQAKVYADGQLIGEHKG
                     GFTPFECLIPSNLYDADQFRLTVCANNELNYTTLPVGNYSEEVDENGQIVKTVKENFD
                     FFNYAGIQRTVHLYKRPQNRIEDIVIRTELNQDLTQAVVNVDVKTVGQYDSIRMSILD
                     QEGQEVGLLENGQMVIEHPRLWEVLDAYLYTAKVELFDGENLLDTYSEQFGIRSVAVE
                     NGQFFINGKPFYFKGFGKHEDTFINGRGFNEAANLMDLNLLKDIGANSFRTSHYPYSE
                     EMMRLADRLGIVVLDEVPAVGLFQLFNAALNLTGDSEEVKSTWEVMQTKDAHELVIDE
                     LIARDKNHPSVVMWVVANEPAGHEKGARAYFEPLIQRMRDRDPSKRPVTLVNIMTATP
                     DKDEVMDLVDVVCLNRYYGWYVAHGDLKAAEKGLRQELETWQKLYPDKPILITEYGAD
                     TLQGLHSMWDIPYTEEFQVDYYDMNHRVFDSIPNLVGEQVWNFADFETTVGIIRIQGN
                     HKGLFSRNRQPKQIVREIKKRWTAIPNYHYKRK"
     gene            complement(17658..18686)
                     /locus_tag="AC1PNG_000144"
     CDS             complement(17658..18686)
                     /locus_tag="AC1PNG_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIP6700687.1"
                     /translation="MKKILAFGEVLLRLSPPQYQTLTQATSLDCQFGGSELNVLASLA
                     QLGHSVELVTALPKNEVGKMAEHFLFARQIGQKYVIRNEGRLGLYYYQKGFSLRPSQV
                     TYDRDYSAFSLSKEEDYDLEGMFDGVDWFHVSGITVALNPDIYRLAFRLMEMAKGQGV
                     KVSFDLNYRESLWSSFEEAREKLSPFVGLADICFGLEPIQLFNESGKDLKDELGLKRP
                     YENKEVLLAVVKGLAETYSLQGLAFTQREMTYNNEYLLKAYLYQDGILYETEKESIQV
                     LDRVGTGDAFTAGIILGYLQEKSPREILDLGMASFQFKHTIEGDINVISQSDIDLLLK
                     KGSREIKR"
     gene            complement(18742..20295)
                     /locus_tag="AC1PNG_000145"
     CDS             complement(18742..20295)
                     /locus_tag="AC1PNG_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP6700688.1"
                     /translation="MEKHVEQRVEDVTYNKAKLWQIILFAMNNSSTNIYLVAFTFVTY
                     LSTGVLGLAALFVSQLMGYIRIFDGFIDPTIGVIIDKTDTKFGKYRPILVLGNIITAA
                     SLFMMLGLSGVNESLRLPLFILVLIIHKIGYSLQQTITKAGQVALTNDPKQRPIFNIV
                     DGIATTVLMTGSQVVVSGTLVPKHGGFTPSFFQELFLGVVVISAVLGILAIIGIWEKD
                     NKKYFGLGEKTQETKLKDYWKVIKGNKPLQVLSISAAFVKFVAQLFGDSVIGVMLYGI
                     IFGDYALSGKMSMLLILPGILVTVGAAQLARRKGLRFAYITALQLGIAGLVILAAMLF
                     SAKPGMLDLTNWNLYTIGFFVVYIVARYASSAPSGLVLTMGADITDYETSVSGRYLSG
                     MIGTIFSLTDSIASSFAPMVVGFVLAGIGFGKEYPTVETAFSTDLRMALIVLLTVIPA
                     AVLSVSLFLMKFYKLDREEMARIQEKIHVMKAAKDEERVRAIAKNVPLSDMDYVDVTQ
                     YKVDQYDDK"
     gene            complement(20482..21321)
                     /locus_tag="AC1PNG_000146"
     CDS             complement(20482..21321)
                     /locus_tag="AC1PNG_000146"
                     /EC_number="3.1.30.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HIP6700689.1"
                     /translation="MAKKNVRKQAMSLLSLLATIVVVAGGYLLSDNKSSNVADYSYYV
                     NQSKASEGTPSQELASSVLTEDVKKQLGNSIEWNGAGAFIINGNQTDLDASVASVPYG
                     DNKTKTVQGETVPTVANALLAKSTRQYKDREETGNGRTSWTPAGWHQIHDLTGEYDHA
                     VDRGHLLGYALIGGLSGFDASTSNPKNIAVQTAWSNQANDQTSTGQNYFESQIRRALD
                     KNKRIRYRVTLVYAESEDLVPVGSHLEAKAADGSLEFNVFVPNVQSGLSINYHSGEIA
                     VNQ"
     gene            complement(21351..21542)
                     /locus_tag="AC1PNG_000147"
     CDS             complement(21351..21542)
                     /locus_tag="AC1PNG_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIP6700690.1"
                     /translation="MDKDNLSYVGKNLILVAVVLLIAILVFVLGLMVGYGVIGDGDNV
                     FAVLSPAKWQELIGKFTGK"
     gene            complement(21535..22824)
                     /gene="murA"
                     /locus_tag="AC1PNG_000148"
     CDS             complement(21535..22824)
                     /gene="murA"
                     /locus_tag="AC1PNG_000148"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIP6700691.1"
                     /translation="MDTIIVTGSKNGLKGQVEIEGAKNAVLPLLAATVLASQGQTKLT
                     QVPILSDVYTMNNVVRGLGVRVKFDEETKTIDIDATGKLGSETPYKYVSQMRASIVIL
                     GPILARNGYAKVSMPGGCTIGSRPIDLHLKGLQAMGAKIKQRAGYIEASAENLQGAHI
                     YLDFPSVGVGATQNLMMAATLAQGTTVLENAAREPEIVDLAIFLNKMGAKVKGAGTET
                     ITITGVEELTGAEHQVVQDRIEAGTFMVAAAMTGGDVLVKDAVWEHNRPLISKMLEMG
                     VEVTEEAEGIRVRSQVDKLKPVTVKTLPHPGFPTDMQAQFTALMAVAKGESTMIETVF
                     ENRFQHLEEMRRMDLHSEILRDTAIITGGLALQGAQVMSTDLRASAALILAGLVAEGE
                     TIVGKLTHLDRGYYRFHEKLAALGATIERVSGEDENG"
     gene            complement(22849..23073)
                     /locus_tag="AC1PNG_000149"
     CDS             complement(22849..23073)
                     /locus_tag="AC1PNG_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HIP6700692.1"
                     /translation="MVISILQFFSHILFIYLAHHLLVTTVDWSRWLKVTGDNQRKINL
                     LILFLAIALGYLVSTFFLELLMVGRSFANM"
     gene            complement(23133..23549)
                     /locus_tag="AC1PNG_000150"
     CDS             complement(23133..23549)
                     /locus_tag="AC1PNG_000150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HIP6700693.1"
                     /translation="MGQMTVQIVTPDGIKYDHHAAFVLVKTVDGEMGVYSGHEELIAV
                     LEIGEMKVRRVDDENHVDWIAVNGGIIEVNKDIITVISDSAERERDIDISRAERAKLR
                     AERELEEAQTARNIDMEMRATVALQRAINRIRVGKH"
     gene            complement(23559..24965)
                     /gene="atpD"
                     /locus_tag="AC1PNG_000151"
     CDS             complement(23559..24965)
                     /gene="atpD"
                     /locus_tag="AC1PNG_000151"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIP6700694.1"
                     /translation="MSSGKITQVVGPVVDVAFAAEDKLPEINNALVVYKNDDSKQKVV
                     LEVALELGDGVVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EESFPADFEREPIHKKAPAFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     LGIYPAVDPLASSSRALAPQIVGEEHYAVAMEVKRVLQRYQELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAEQFTGMPGSYVPVAETVKGFKEILDGKHDHLPEDAF
                     RNVGSIEDVVAKAAKMKF"
     gene            complement(24985..25863)
                     /locus_tag="AC1PNG_000152"
     CDS             complement(24985..25863)
                     /locus_tag="AC1PNG_000152"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIP6700695.1"
                     /translation="MAGSLNEIKSKIASTKKTSQITGAMQMVSASKLAKSEQLAQSFQ
                     IYASKVRQITTDLLRGELMASDTSNPMLIRRPVQKSGYIVITSDSGLKGSYNSSILKA
                     VMGMIEQDHDSKDEYEIIAIGSMGADFFRARGINPVFELRGLADNPSFEEVQKIISKS
                     VEMYKNELFDELYVCYNHHVNSLTSQVRVQQMLPVEDLDHNEADGYTATFELEPNREV
                     ILEQLLTQYAESTIYGAILDAKTAENAAGMTAMQTATDNAKNVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(25875..27395)
                     /gene="atpA"
                     /locus_tag="AC1PNG_000153"
     CDS             complement(25875..27395)
                     /gene="atpA"
                     /locus_tag="AC1PNG_000153"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIP6700696.1"
                     /translation="MESGVLLINAQEISALLKQQIEGFQPDFDYTETGVVTYIGDGIA
                     RAQGLDNAMSGELLVFENGTIGMAQNLETNDVGIIILGQFTDIREGSVVRRTGKIMEV
                     PVGSALIGRVINPLGQPVDGLGEIRTSKTRPIEYPAPGVMQRKSVNEPLQTGLKAIDA
                     LVPIGRGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLR
                     QYGALDYTIVVTASASQPSPLLFLAPYAGVAMAEEFMYEGKHVLIVYDDLSKQAVAYR
                     ELSLLLRRPPGREAYPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAY
                     IATNVISITDGQIFLKDDLFNSGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLA
                     SYRELEAFTQFGSDLDAATQAKLNRGRRTVEVLKQPLHKPLPVEKQVLILYALTNGFL
                     DSVPIDDILAFEEELYSYFDLHYDGLLDVIRTTKDLPDTDELNAAIQAFKDQSVFK"
     gene            complement(27399..27932)
                     /locus_tag="AC1PNG_000154"
     CDS             complement(27399..27932)
                     /locus_tag="AC1PNG_000154"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIP6700697.1"
                     /translation="MNARENAIVQKYALSFVEKVSDHADIWDMYDQISDLISIIHDSK
                     LNRILLSATVSREEKADFVRTVRQSSFWQINDLIEDVIRDGHADLLLETLERVQLQIS
                     KFKNEFEARVVSVYPLTEAQKERLRHLVEQRFSLRVRNITEELDQSLLGGFIVTVNHK
                     VIDASVRTQLKDVRKKL"
     gene            complement(27933..28439)
                     /gene="atpF"
                     /locus_tag="AC1PNG_000155"
     CDS             complement(27933..28439)
                     /gene="atpF"
                     /locus_tag="AC1PNG_000155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIP6700698.1"
                     /translation="MATTITMQSSTILGNFILVTASFAVLIILIRVFAWDKITGIFEE
                     RANKIANDIDAAEEKLTAAANLVQQREDELVQGRIESQKIIQDAVERAKLEKKRILEQ
                     ADVEIQGLKQKAQLEIEAEKREAQENLRVQVAELAVDLASKIILEDLDQQAHSNLIDR
                     YLDKLGDK"
     gene            complement(28457..29173)
                     /gene="atpB"
                     /locus_tag="AC1PNG_000156"
     CDS             complement(28457..29173)
                     /gene="atpB"
                     /locus_tag="AC1PNG_000156"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIP6700699.1"
                     /translation="MEEHLSPTLTLGPVTFDLTMVLVSVITISIIFLLVFWASRRMEL
                     KPKGKQNVLEYVYELTINFTKGNLGDAEAKRYSLFFFTVFTFLLVANNLGLMTKLETA
                     EGHNLWTSPTANMAYDFGLATIATVFCHVEGIRRRGFKAYLKSFVTPWAMAPMNILEE
                     VTNLVSLALRLYGNIYAGEVLVSLLLQLSQQSALAYPIAFALNVVWTAFSVFISCLQG
                     YVFIMLVSMYLNKKISSESE"
     gene            complement(29210..29410)
                     /locus_tag="AC1PNG_000157"
     CDS             complement(29210..29410)
                     /locus_tag="AC1PNG_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HIP6700700.1"
                     /translation="MSLGALALGLACLGVSIGEGLLVASYLSSTARQPEMQSKLMAGV
                     FLGVAFIEGTFFVTLAMTFVLK"
     gene            complement(29547..30590)
                     /locus_tag="AC1PNG_000158"
     CDS             complement(29547..30590)
                     /locus_tag="AC1PNG_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HIP6700701.1"
                     /translation="MEERKRARLIYNPTSGQEIMKKNVAEVLEILEGYGYETSAFQTT
                     AEKDSAKNEATRAALADFDLIIAAGGDGTINEVVNGIAPLEKRPQMAIIPTGTTNDYA
                     RALKVPRGNPVEAAKVIGKQQTILMDIGLAKNQKNGFHQEHYFINIAAAGTLTELTYS
                     VPSQLKTMFGYLAYVVKGAELLPQVQFTPVRVEHDEGVFEGSVSMIFVALTNSIGGFE
                     QIVPDAKLDDGNFTLLMVKTGNLFEILHLIRQVLDGGKHIESDLVEYIKTKSLSIENL
                     NPDNRLLLNLDGEFGGEAPVRLHNLSNHIEFFADTDLVSDHAITLDTDQMNREDMAKR
                     FIEETDHIDEAIK"
     gene            complement(30601..32559)
                     /gene="ligA"
                     /locus_tag="AC1PNG_000159"
     CDS             complement(30601..32559)
                     /gene="ligA"
                     /locus_tag="AC1PNG_000159"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIP6700702.1"
                     /translation="MKTRISELVSVLNQYAKEYYQLDQPSVSDAEYDTLYRELVELET
                     AHPELILPDSPTHRVGGKMLDGFEKYSHVYPLFSLQDAFSREELEAFDQRVRKEFPQA
                     TYICELKIDGLSISLTYEAGNLVVGATRGDGSVGENITENLKRVADVPLTLPEAVDIT
                     VRGECYMPKASFDRVNKQRQEAGEAEFANPRNAAAGTLRQLDTGVVAQRGLATFLYQE
                     ASPSEATSQSQVLEKLDALGFVTNHEYCLAESIDDTWDFIEKIAERRDDLPYEIDGVV
                     IKVNDLAIQEELGFTVKAPRWAVAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLSPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLKVVDKYRKEQEIMPIP
                     SHCPSCQSDLQHYEDEVALRCINPICPSQLMSKLEHFASRDAMNIAGLGSSIVEKLFG
                     AGLVHDVADIYKLTVDDLLTLEGFKEKSADKLYQAIQTSKSNSAECLLFGLGIRHVGS
                     KASKILVEKFGDLETLAFADQEAIASLEGLGQVIAKSLTTFFASEGAQQLLAELKEAK
                     VNLTYLGQVVDENAALSGMTVVLTGKLERMKRNEAKAKLEALGANVAGSVSKKTNLVV
                     AGTDAGSKLTKAQELGIEIKDEAWLESL"
BASE COUNT         9324 a         7835 c         6024 g         9401 t
ORIGIN      
        1 atggatagac taactccaaa aaccatggac atctgcgcta aggtaatatt gctattggca
       61 gttgaaaaga tactattaaa caactgcatg ttgacttact tcctctccag tgatgttgtg
      121 ggcaagctta taggcattgc catattttga aaatgattgc ttttcgattt ggtatttttc
      181 gagcagggct accaaccagt ctggtcgttc ttctggaact tttacttcca tgattacttt
      241 ggttgggtct aataggttct taccaaattt accttccatt gcatccacat cgtagtctcg
      301 ataaagtaag ttcttatcga ttgtgagacg aacttttttg tcttcgattc ctttatagga
      361 tacgcgatcg tagtagatat acatcttcgg cttaatagtc ccgtagcgtt ctctcaacat
      421 ttcgagttct gagtttacct tatcatcctt gattgtggaa tcaatcactc cattttcgat
      481 ataatttgct acagaaacag ggtttgaagt taaacggtat ttatagccaa ttttattttc
      541 ttttttctta atttcaagaa aggcttgact cgattcagat ggtatcgcat catagacacg
      601 catacgaatt ttctcacgtc cattcttttt ggcaagtgaa tcttgaatca tatcaaaatc
      661 ttcattatca aagtagatat tggtaatcgt tgatgtcgca aactcatctg ccatcatata
      721 ctgacgaagt tccttttcta aaagttcaaa agttttttta tcaacgatat atttagtctc
      781 tttacgctta aactgctttt gtacgattct tgttttcatt attgtgcctc ttctatccta
      841 aatttttaca ggtccctgtc ttgtttacaa gatatagtct accgacttag ctttaaatga
      901 aactgaaaat aaaattaaac aaaacttaaa ctgcaataaa gttgaaattc ttttgtcaca
      961 ttctaatcta tgctacaata gtcctatcaa tagtaaggca aggagaaacc catgtacgaa
     1021 tttgtcctag aatacggctc tttccccgta aaactcatcg acggttttgt caacaatcgc
     1081 tcagaaattc ctgattttct caaagaagac gaagaaatga ttgcccgctt gaacgagatc
     1141 aacgaactct tccaccaact tttcttaacc atcgaatgca agtttgacta tatcgggaaa
     1201 caattccctg acaagatcga acaactacga gaactctatc atcccctggc agacgattta
     1261 ctagccaagt atggcaacca gatagagttg aagattgaac cctttatttt gtaagacaaa
     1321 agagatgacc gcaatcatct cttttcttct attctgtatt ccaaaccata ctggtcacgc
     1381 gataggtaac cataatccac caagtagcgt ctcaatatag catagtcatc ataaatctca
     1441 gctagaaagg cattaacttc tttttctgtg aatgccgagc ctttcttagc caactcttct
     1501 tgtaaatagg caaacaagac ctgcttactt tttaattttt taggaataac gataagcttg
     1561 ccttctcgaa aaaatttttc ttgaatatct tgagtgttca tacttccctc caaaaaatcc
     1621 tagcccgctg actaggattt cacaagattt acatgttaac ggatttcagc tccaagctca
     1681 accaaagcct tactaatttt ctccatatac ttagccactt cctcatccgt caggctatcc
     1741 gcaggatttt ggaaggtcag gttataagcc atagacttct taccagcctc aatattgcta
     1801 cccgcataga catcaaagag ggtcacttta gtcaagcgtt tcacgccagc accagcaatg
     1861 gcatccagga catcctgatg tttaacatca ctgctcaaga gcagggcaat atcacggcta
     1921 accgctggga atttagagat ttcggtaaat ggctgagcag gctggagagc ttcttcaatc
     1981 gcatccagat tgatttcagc cacataggtc tcaggaatac cgtagtcctt ggccgtttga
     2041 ggatgtacct gaccgacaaa gccgatcaca gccccattca aatggatttc agctgtacga
     2101 ccagggtgca aggcagcaat ctcactagtc gccacaaagt caacagccaa cttataatga
     2161 ccaaagattg cttccaaaat ccctttggca tggaagaaat caactggtac agctggtgtc
     2221 tggaagtctt tctcagctac caaaccagtc aagacaaggg caaacttatt gatttcttgt
     2281 ggcaggtcct cttttggatt gccagactgt tcaaagattt taccgatttc gtaaagagcc
     2341 aagttcttgt tcttacgagc cacattgtag gccactgtgt ctaacatacc agacaccatg
     2401 ttctgacgga gggcagagcg atcaacagtc ataggccaca tgagttcggt gactgtggtt
     2461 ggacgagccg caaactccac agccttttca ggcgttgtca gagcgtaaga aatgatttct
     2521 gtcagacctg caccttctgc caggctacga acttgacgac ggatgttctg ggtcagggtc
     2581 aactcacctg ctgttccgtc ctctttcgga agagtggtcg gcagattatt gtaaccatag
     2641 atacgagcga tttcctctac caagtctgct ggaatgcgaa tgtcccaacg acgacgcggt
     2701 actgctactg taaactgact tgcatcgcca gttgtttcca tacctaaacg acggaaaata
     2761 tctgcgattt gggcatagtc cagattggtc ccgagcgagc ggttgacgta atcaatggtc
     2821 gctgtgactg ggacatccga agtatccacg ctgcccgcag aaacgatgcc agacaagacc
     2881 tgaccgccag ccagttccgc aatcatagca gccgctgtat ccatagcctc cgtcaaggtc
     2941 gctacgttga tgcccttttc aaatcgagaa gaagactcag agcgaaggtt gaggcgacct
     3001 gaggtcttgc ggatagactt gccatcaaag agggcagctt ccagcacaac cgtcttagag
     3061 ttgttgtcaa tttctgtatt agcaccaccc atgactccac cgagggcaac cgccttgtcc
     3121 ccaacggaaa tgacgatgtc atcggtaatc aagtcacgtt cttcgccgtc aagagttacc
     3181 aagttctcac cctcttttgc ctgacgagcc acgattttct tgccatcaaa cttgtcaaag
     3241 tcaaaggcgt gcattggctg accgaagtag agcaagatgt agttggtgat gtccaccaca
     3301 ttgttgagcg ggcgaatacc tgcgttcatg agcaggtttt gcaaccactg cgggctcggt
     3361 gcgatggtca cattttcaat gacacgagcc gtgtaggcag ccaccttgtc tgactcaata
     3421 gccacttcaa tcacatcgct ggccttcttg tcgctttctt ccaagaccac tggcttgaag
     3481 ttgaccttgc tgtcatagat agccgccact tcgtgagcca cgccacgcat agagagagca
     3541 tcggcacggt ttggtgtgat ggacagctcg ataatctcgt cgtcgaggtc caggtatgaa
     3601 aagactggct cacccacgac tgcatcttct ggcaggtggt agattccatc cgcatagacc
     3661 tttggcacaa ctgagtcgga cacgccgatt tcgctcaatg agcagagcat ccccaaggac
     3721 tcaacaccgc ggattttgcc tttcttgatc ttgtagttgc cagcgatacg agctcctggc
     3781 agagcgacga tcaccttgat cccaggcttg acattcggag caccgcaaac gatttgcgtg
     3841 atttcgtcac ccacattgac ctggcatgta ttgaggtggg tatctggaat tggctcagca
     3901 gacacaacct gaccgacaac caacttagac aggccagcac tcttttgctc aaccccttct
     3961 acttcaattc ctgttgttga cattttttca gacaagtcat gacttgtcgc atcaaagtca
     4021 accagttctt ttaaccattt gtaacttact aacatattat cctttctggt gcaaaggccg
     4081 tttcagtagc caatccacat caatcttatc accgaccgca aattcatgct ggccaaattt
     4141 ttcaaatccg tatttggaat agaatttctg agccttgtaa ttatgttccc aaactcccag
     4201 ccagacccag tcacaactag actgctctgc tttttccaaa gcaaattcaa acaagacctt
     4261 cccaagaccg cgaccttgaa aagcctgcaa gagatagata cgctggattt caaaggcatt
     4321 ttccaactcc tgctccgtct gggcaggacc ctggttaagc ttaagataac cagctatctg
     4381 atgatctatt cttagaaaat agtgctggca gttgggatcc gataactcac tagtcaagac
     4441 ctccaaggaa taagactcct caaagaatgc ctccagttcc ttcgggtcat tatcgtgagc
     4501 gaaggtctct gcaaaagtct gcttagccag ctcctgcaaa acagaaacct cttccaaaca
     4561 tataggatca atctgcatct cattgcctct catttctaac tgggctagga aagtcgctca
     4621 atagcgattt ccgttcacct actccagcgg tgttacgacg gttcaaggtt gaaaacaaca
     4681 tttttttcaa ccttgaacct agtgagggga gtaggtagac cgccatagcg gctaccgttt
     4741 ctcgtactga acacctaaca aacttcctaa attacgacgg atttcaagca aaacgatatt
     4801 tttagcttga aatcctagta aggtagctag gaaagtcgca gagcagctat ttccacacct
     4861 taaaatagtg cgagattacg acggtatttc aagcgaaacg atatttttcg cttgaaagcc
     4921 tagtaaggta gctaggaaag tcgcagagca gcgattccca caccttaaat agtgcgagat
     4981 tacgacgttc taaggttgaa aacgacattt ttttcaacct tgaacctagt gaggggagta
     5041 ggtagaccgc catagcggct accgtcctgt ttctagatac tttcagtatc agaaacagct
     5101 ctcacatcaa aactgctccg agaatcgtac atcgccttga tagaatccac gaatatcgtt
     5161 gataccgtag cgaagcatgg caatacgttc ctgaccaaga ccgaaggcaa agccagagta
     5221 cttggttgag tcaatgccac tcatttccaa tacttgcggg tggaccatgc cggcaccgag
     5281 gatttcaatc cagcctgttt tcttacatac attacagccg tcaccaccgc acttaaagca
     5341 ggaaacatcc acctcaacag aaggctcagt gaatgggaag tatgatggac gcaggcgaat
     5401 ctgacgctct tcaccaaaca ttttcttgat aatcatttca agcgtaccct tgagatcgcc
     5461 catggataca ttttcaccga caaccaaacc ttcgatctgg tggaactggt ggctgtgagt
     5521 cgcatcatcc gtatcacgac ggaaaacacg tcctggcgaa atcatcttca aggcaccttt
     5581 agaaaaatca tgttggtcca tggtacgtgc ctgaacaggc gacgtatggg tccgcataag
     5641 aatttcttct gtgatgtaga aggtgtcctg catatcacgc gctgggtggt cttttggcaa
     5701 attcatgcgc tcaaagttgt agtagtcttt ttccacttca aatccgtcaa cgatttggaa
     5761 gcccatgccc aagaaaatat cttcgatttc ctcagaagtt tgggtcaaga catggcgttt
     5821 tccgacctta acctgacgac ctggcaaggt cacatcaatg gtttcggaag ctagttgttg
     5881 ctgaattttt gctgctgcaa ccttttgagc agtttcttca aaggcagttg tcagcacatc
     5941 acgcacttca ttaacctgtt taccaacgat tggtttcatg tcgttagaca ggtctttcaa
     6001 gcctttcaat aagtccgtca aagacccttt tttacccaaa accgcaacac gcaggtcttg
     6061 tagttctttt tctccttggt gctggatttc ttttaacttt tctagtgtcg tttggcttaa
     6121 ttcagccaac tgttgttcaa tgtttgacat aattcctcca ataaaaaacg catgacacca
     6181 ctcgtatcgg agcgttgtca cgcggtacca tccgttttca tctgcaaggc agaccttagt
     6241 tctatgaacc tatattcaca agtgaagtgc ttgcacataa gagatggttt tccgctaatc
     6301 aaggctgttt tttgagggaa tactgactgt attttgaaaa aaactaacga tgagtagctg
     6361 aaaaactagc cttagatgga agtgttgaac tgtgaatgca ggttctaagt ccttgtgtga
     6421 gtgaattcga ccaactttca ctgattgagc ttacagtcac ggctcaactc cctgggcagt
     6481 ttccataggt gtactagtct cacagactcc tattcaatta agaatattct atcaaaaaga
     6541 agaaagtttg gcaagaaatc ttcctagaaa actccctcta tctaggatag aaaggagttt
     6601 agttttatag ttttgcctgc catttattgg cccaaacatt ctcatttgga acaccataga
     6661 agtcatcttt tccttctaat ttatcaagta aggattcgat agtatttata ttgtgatgat
     6721 aggcattgat agcagttttg accatggtaa catcgtgaag gtgagttgta tagtttagtg
     6781 aaacgaaaat ggtcggaact tcatgtacat accaaggaac ttcattggac atagccgttt
     6841 tccactggat gcggtaatta ttctgtgctc catatccaat gacattggca acagtcaagg
     6901 ctaaatcaac ttcttctcgg tattgcaagg tttttccttt aatccgagtc gtaccatcat
     6961 tgacagtcac ttcataacca cgcgcttcca aggcagcttt aaggttatca accacttctg
     7021 tacttgccgc cataatccct tctttttccc cttctaggta atacaaacgg attctgcgat
     7081 gggtttctgg actgattgga agattttttt gtgtatcttt caacagcgta attgccttgt
     7141 ctgctgcctc acgttgccaa gccaagtgtt cttcacagcc gataatagcc aattcatcag
     7201 ctgattttag taaggttcct tccgcttgct tgatatgcag attgagtttg gctttcagac
     7261 ctaaaatgcg acggagagca tcatgaaggc gttcttcagt aatcactccc ttgcggtagc
     7321 cattgagcat aaattcaaaa tcttcttcta agttattgaa gaataagaac atatcgcaac
     7381 ccgcagcaat ggctgcaggt acgtagtctt cacgacgcat ggctgctgtc atccccaaca
     7441 tgtggctggc atctgttatg accaagccat tgaattccag tttttctttc aataaatctt
     7501 gaatcaattc aggagccagt gttgcaggca aaatgtcttc atccttcaaa gttggattga
     7561 gtcgttgttg gtaatatggc aaggcaatat gacccgccat aatcatctca acaccagctt
     7621 caatatgatg acgatagaca cgaccaaaac tcttgtccca ttcttctgga gagagttcat
     7681 tgacaccgag gactaggtgc tgatcacgtt cttctgtacc atctcctggg aaatgcttga
     7741 tacattgaat aacatctctt tcagcagtca atccttttaa ataggcactg gtataggcaa
     7801 taacatcatc cgcatttgta ccataggctc tggtattgac aatcgtattg cgccagttct
     7861 gaacaatgtc cacgcaagga tcaaagttga catttacacc taatgcagtc tcctcgcgcg
     7921 cagatacgag acctgctagg tagggaatac gaggatcccg actggcttct gattgagcac
     7981 ctgaggaaat ataggttcct cctttcacag ccccatctcc tcctgagtca cagttcgctg
     8041 caaccaagag aggtattttt gaatagcttt gcaactcgtt aatcagtcct tgtacctgtt
     8101 ctggactgcc attcatataa cgagcaccac ctatgtgata tcggttcatc aattcctgat
     8161 tactcagatt atttccacta aaggcatccc ctccgaagaa gaatagatta acaaacaatt
     8221 gaccgatttt ttcttcatca gtcagatgag ccaaggtatc ttctacccag gtaatctgct
     8281 catcattcaa acaatagggt ttcttccgca aatcaaccaa tctaggcatt ctttcctcct
     8341 tattttccgc gccaaatctg tgtatctacc aagccacaat aggcatctac aggcgatact
     8401 cctgtaaagt ctgactctcc tgctagttta gcaaccaagg cttctaatgt gacaggcaag
     8461 ccatcatagg cattgatata ggtagcaacc tgtggcatat ctgcaagtgc aaatgggtgc
     8521 tgaacagaga caacaatggt tggaatttcg tgaacataga aaggctggtc tggcgttccc
     8581 ttggcagctg gccaaataat tcgctgagtt gttcctcctg agttgacatc aactaaattg
     8641 ataatcaaat catagttatc tgtgatttcg gcaattggac gcttggaagc atagacattt
     8701 gcaatcgctg ctggtctttc ttcttctggt aatttggcaa tccgtgcctc cgtattttcc
     8761 cagatagata cttggtgacc tcgagcttct aatagttctt tcagcgtatc ggctgctctc
     8821 ttcttgccag cattgatcat ggcgccaaaa ccacccttat agccatccac ttcgaccaat
     8881 aaaatacgtt tgtagcgttc tggatttacg gggaaaattc cttcttgttt ggcttttacc
     8941 aaggtaatcg ccttgtcagc tacttctttt gcaagctgtt tcgcctcatc tctaccaatc
     9001 aaagccagtg cttcttcttt tggcatcatg atttcctgac gacgaccttc aaacaagtga
     9061 agaccgagtt tagctttcaa gcccaaggta cgacgaaggg catcatgtag acgttcctca
     9121 gaaagcagac cattttccaa tccttctttc atccattgca aatcttcttc tggatcattg
     9181 aagaaaagga aaagatcaca ccctgcctca atagctgtag gcaatagctc acgacgcgac
     9241 atagaggctg tcataccgat catgtgactg gcatccgtga caatggcacc attgtaacct
     9301 aattctccac gaagcaattc atccaagagg gttttattca gcgaagctgg caacatgtca
     9361 tccaattcac gttctggatg catttctttt tcgacatttg gcaaatgaat atgccccgcc
     9421 ataatacctg gaagacctgc gtcagccatc tcaccataaa tccgtccaaa tgtttccatc
     9481 cattcagatt tggtcatggg attcgaagca aaagagagat gatggtcacg ttcatcaata
     9541 ccatcgcctg ggaaatgctt agcaaacggc acaatgccat gttccataat gcctcgcatg
     9601 tattctttgg acaattcaat gacttggtct acattagctc cccaagttcg gtttgcaatg
     9661 attggattgc gccaattacg gctgaggtcc ataatcggtg caaaggaggc attgcagcct
     9721 actgcagctg cttcaagtcc tgcaactttc cccatttcat aagcatagcg gggatcagct
     9781 gtcgcagcaa tcttgatttc atccccaatt ttcgttccgt ccgtaacagc gccattccca
     9841 ccagattcgg tattggctgc aatcaaaagg ggaatcttag acttggtctg caaaatatag
     9901 ttttgctccc aaatatctgc agcaggtcca ggattgtaac gaacagcagc aatcttataa
     9961 tcatttaata ctccagtcag atattcttct gtccgctggc tccccatgtt gacaaagagg
    10021 tgaccgattt tttcatccaa ggtcatctga gcaatggttt cttcaatcca agaaatagcc
    10081 tcttcatcaa ggctgtaagg tttcttccgt aaatctacta aatgtgtcat gtttgcctcc
    10141 aaaaaagcta gagggcatcg cgcctctagc ccttctctac tagtcgtcta aattattcca
    10201 aaatgcctgt atcaattctg tttctttctc acctgtgaat gcttgatgag caaattcatc
    10261 tgcaatcgtt tctgtcaaat ccgcccaaga ttgcatctct tgtcctacta aaataggata
    10321 gaaatggaca ccgttcaatt ccgccgcctt caaatcacca ggcgagtcac caatcatcat
    10381 aatcttatca ggacaatacc cttcctctat taatatttta atgacatctt ctttcttgcc
    10441 acggtcttga cagtacaatt ctgtaacaaa gtccagcaaa ccttggtctg tccactcttc
    10501 ttctactgct tccttattag cagaagaaac cacgtagact tttcctaact ggctcaattt
    10561 ttccaagccc ttgtgaacgc cgatgaaggc taaaacagga ccgctataat gcttaatggc
    10621 ttgattgact tgagtggacc attctaaagc taattccaag tcctttgatg gcgtctcctc
    10681 tatcaatctt tccagagaag catttgacaa agaatctgtg gtcgcgaccc aatttttcaa
    10741 gttatctata ccagtcacac ctgcaaagtc taggccttta actaagccta caaaacggtt
    10801 aatgccacgt tctcgagagt acagattgac tcgattccat tcagctaaaa acggttctct
    10861 atctttgaca tcaaaaacat ctgcagccaa ggggccgaaa aagagtttat gtttgtaggt
    10921 catggtgtcc atggcgcagc catcagagtc cacacaaaat acgtaatccg ttttcatcat
    10981 caaactcctg aataggctga gaagccaccg tcaatcggta aaacaacacc gttgacaaaa
    11041 cttgccaaat tctcatctgc caagaagaag actccgccga ctagctcttc cgcttcacca
    11101 aaccgtccca ttggtgtatt ggtcaaaatc ttgttggcac gtgcggtcgg ttgacctgac
    11161 tcgtcaaata gcaagccacg gttttggttg gtaaccaaga atccaggcgc aatcgcatta
    11221 caacggatgc caacttttga aaaatggact gccaaccatt gggtaaaatt actaatagca
    11281 gccttggcac ctgaataggc tggaattttt gtcaaaggag taaaggcatt catgctggaa
    11341 atattgataa tatttgctcc cgagcgacca atcatatcct gtgcaaagac ctgtgttggc
    11401 aataaggttc ccaaatagtt caaattgaat acaaagttga tacctgcctc atccaagtcg
    11461 aagaaagttt tggtatcagc tggtagtcca atctcatgga attcattgtc cgttgtcgct
    11521 tttggattat tgcctccagc accattgatt aaaatatcta caggaccaaa atctgctagg
    11581 acttgctggc ggactccttc caaattttcc ttagaaagaa cgtttgattt ataggctttt
    11641 gctacgcctc ctgctgctgc aatctcatca acgaagacct gagccgcagc ttcatttaag
    11701 tccaaaagcg caacctttgc acctgcctta gcgaattctt ttgccaaata gccacaaaga
    11761 acgccacctg ctcctgtaat aacaacaacc ttgtccttaa attcaataac acgtgtcata
    11821 ctagctctcc ttatttccga ctgttttgac tgtaattcca aaatctggtg tcttgcctgc
    11881 tgctcgcata ttggcttcat agagaccgtt aaagtaggtt gccccaaggg ctcggtcata
    11941 cagaccataa ccaggtgtct tggtctggtc tccccagata cgacgaccgt ggtctggacg
    12001 caaggcacca gtccagtcat aatctaccaa taatttaaca acagcgttca tgtcaatgtc
    12061 accagcctgt gacagatgtg ctgtttcttg gaagccccaa gcccctgctg tcacattgcg
    12121 cgtatgcata aaattgatac gattgcgttt gagagcatat tccgtcatgg caaggacatc
    12181 atttttcgga tcagaagcat aggatccgac acacatggta attccattgt gctctgaatc
    12241 atagagattc aaaaatcgtt caacggcttc ttgaccagta ataatacggg gcaatccgaa
    12301 aattccatac ggagggtcat caggatgaat ggccattttg acgccagctt cctcagccgt
    12361 tggtagaatc gctttgataa aatactccaa attcgcccat aaatcttctt cagagatatt
    12421 ctgacgataa ttttcaataa tcgccttcat ttcttccttg gaataggacg aatcccaacc
    12481 tggtaagttc aaatcatccg ccacaggatc aacacctgct aaatcagatt taaggaaagc
    12541 taaagaagtt gaaccatctg gaagtgggtg gtgcaagtct gaacgggtcc agtcaaacac
    12601 aggcatgaaa ttgtagcata caactggaat acctgcagcc cctacattac ggattgaagt
    12661 tttatagttt tcaattaact catcacgatt tggcttccct tgtttgatgt cttcatgaac
    12721 aggaatggat tcaataaccg taatttcaag tcctgcctct tcgaccattt ttttcagctc
    12781 tagaatgttt tcaaggggcc aagcttgacc aactggcaca tcatagacag ctgttacgat
    12841 tccctgcata ccaggaattg ccttaatctc ttccagtgtc acaggatcct ttttcccata
    12901 ccagcgaaat gacattttca tgatttactc tccttctatt gttcaaaaaa ctcttttgca
    12961 ttgtaatagg atatggcttc tacaaattgc cctaaacgat tgtaatcttc tggcacttcc
    13021 tcatcaacta tccattgacc aacataggtc gctaaaatgc gtctgaaata atcatgccgt
    13081 tgataagata aaaagctacg cgaatctgtc aacataccaa caaaattagc aagcataccc
    13141 tgttctgcat aggcattcat ctggtcaatc atgcccaact tggtgtcatt gaaccaccaa
    13201 ccagcaccaa attgtaactg actacgaagc ccttcctcat tggactggaa attcgccaaa
    13261 gtattggcca gagcaatgtt gtaacttgga ttgagattgt accaaatcat ttttggaagg
    13321 gcattttcct tgaccaagtt atctaacaaa cgattcaaat tcacagtcag acatgtctgg
    13381 tctccaatcg aatccacacc tacatctgct ccaagttttt caaacagtcc tgtatggttg
    13441 ttccgtaagg caccaaagtg aacttgtgtc acaaagccat gtttcttgta caatcggcac
    13501 aattctctaa agatagcagt ctgccattta ttgatactgg attggccagc ctctttacct
    13561 gcaagtcttg ctagtaaaat ttcatccaac tcttcgaggg tagcctcttc ataggaaata
    13621 gccgtaaaac tgatgtcact tgctcggcaa ccgtgttgag caaaatagga aacgcgctga
    13681 ccaagtgcct ctacaaaact tgcaaagtct cttaccgcta cccctgttac ttcctccaag
    13741 cgtttcataa atagattgaa atttcgatgc tcgataaagg cttcatctgg gcggaaagtc
    13801 ggtgccacta cagtatctat ggtcgagtcc tcagccaagc gcttatgcca ttccaagcta
    13861 tctagtggat ggtcagttgt cccaataaag gtcaccctag catccgcaat cagcttacgt
    13921 ggactaactc gatgttcttg caaataagca ttgagctgat gatagagtcg ctctgcattt
    13981 tcttcggtca acaattcctc aacaccgaaa acctgtcgca attccaagtg actccaatga
    14041 tagagaggat ttccgaaagc ccgttccaag gttcttgccc aggctttaaa cttatctagt
    14101 ttacttgctg aacctgtgat ttcttcttct gagataccat tagcccgcat caagcgccac
    14161 ttataatggt caccccccag ccacagatca acaatatctt cataaacctt atcttcaaaa
    14221 atttccttag gatccaagtg acaatggaaa tcatagatag gaagctctgc tattttcccg
    14281 taaagctcct tagcaggctc gttttttagc ataaagtttt tgtcattaaa actcatttca
    14341 tccctccaaa gctgcaacaa aactgcgagc aatcgtcgtt acactttcat agccttcagc
    14401 ctgcactcga cttgccagag ccgaaccgac tccaacagca caagcacctg cctttttcca
    14461 atctgcaaca ttttcaactg atacgccacc agaaggcatc aaatctactt ggggcactgg
    14521 tccgtgaata tccttgataa atccaggccc caagacacca ccagggaaaa gtttgataat
    14581 gggacatccc gcctgactag ctgtgacaat ctctgttgca gttgcacagc ctgggaagta
    14641 gaatacctca gcctcttgcg ccaagtcttg aatttcttta tcaaaatgag gactaaccag
    14701 gaaactagca cctgcatcaa tggccgcttg tgccagctta agattcatca ctgttcccgc
    14761 cccgatgact acggaagaat cgtatgagaa ctcctcttgt aattctttga taactgtcgc
    14821 tgcatttgga gtcgaaaatg taatttcaat atttcgaata cctcccaaaa tcgcatggcg
    14881 agcgatttct ttcgcatctt cttcatcttt tccgcgaatc actgcaaaaa agtaattttc
    14941 ttttaattgc tttaacatct tgacctccaa ttagaaagcg ttgtcattca tagctatagt
    15001 atacaattca tcagatgatt tgtcaataaa aaagcaaaaa atacccagaa gaattcttct
    15061 gggctgtcta ttttttattt ctatttatga tcgttgagac catgcaaggt catatcttta
    15121 tcgaaccatt cgcttaaaac aacctgtctt actgatataa tatgtgctaa catgctatca
    15181 tatgctgcaa caggatggcg ttgcttgata gccattaaga tgttcctgtg ggcctgtaaa
    15241 ctatccgatt tcatctgacg atttgaataa ttttcattga tcagatgaat agattgatta
    15301 atgacgggca tcagacttgt catagccaga ttcccactca tctcagccag catacagtgg
    15361 aatttcacat ccaactccaa atgcttcgtg tctccagcag caacggcctc ttcaatagca
    15421 aggacaattt cttctagtcg agcaatgtct tcgtccgagg caaactgagc gacacgctcc
    15481 gcaattctcg gttcaagcaa ataccgcact tcaaacaaat ctgtcgtcaa cttcacacgg
    15541 tctcttacca aagaaaaacc taggggatcc tctgatactc cttttttaga gctgatatag
    15601 gttcccgacc cttggcgcac ctccagaata tttcgagtag ccaaacttcg aacagcttca
    15661 cgaacagtcg aacgtccaac ttctaaatct tgggccaatt catactcatt tggaagctta
    15721 tcaccaactt catatcctct ttccaaaatc aattccaata agcgttccga tgctttttct
    15781 accaagggtt tcgccatgtg cttggtcctc caatacaact ttctatttgc tactattata
    15841 gctcatttcc tcttataatg gtagtttgga atggcagtcc aacgtttctt gatttctctt
    15901 acaatttgct tgggttgtct atttcttgag aataatcctt tgtgatttcc ttgaatgcga
    15961 atgataccaa ccgtggtttc aaaatcagca aaattccaga cttgttcacc cactaaatta
    16021 ggaatagaat caaagacgcg atggttcata tcatagtaat ccacctgaaa ttcctctgta
    16081 taaggaatat cccacatcga atgcaaacct tgaagagtat ctgcaccata ttccgtaatc
    16141 aaaattggct tgtcaggata aagtttttgc caagtctcca actcttgacg gagccctttc
    16201 tctgccgcct tcaagtcacc atgagcaaca taccaaccat agtaacgatt gagacagaca
    16261 acatcgacca aatccataac ctcatcctta tcaggcgttg ctgtcataat attgaccaag
    16321 gtcacaggac gcttagacgg atctctgtcc ctcatgcgct gaatcagagg ttcaaagtaa
    16381 gcacgcgcgc ctttttcatg ccctgctggc tcattggcga caacccacat cactacggat
    16441 ggatgatttt tgtcacgtgc tatcaattca tcaatcacca attcatgagc atccttggtt
    16501 tgcataactt cccaggtgct ttttacctct tcagaatccc ctgtcaaatt caaggctgca
    16561 ttgaaaagct ggaataggcc aactgctggc acttcatcaa ggacgacaat tcccaaacga
    16621 tctgccagcc gcatcatttc ctcagaatag ggataatggg aggtcctaaa ggaatttgct
    16681 ccaatatctt taagcaaatt caaatccatc agattggctg cttcattgaa accacgccca
    16741 ttgatgaagg tatcttcatg tttaccaaat cccttgaaat agaaaggttt tccattgatg
    16801 aaaaactgcc cattttcaac tgcaacacta cgaatgccga attgttctga gtaggtatct
    16861 agcaagtttt ctccatcaaa taattcaact ttagcagtat agagataggc atccaagacc
    16921 tcccaaaggc gtggatgttc aatgaccatt tgtccatttt caagaagacc tacttcctgt
    16981 ccctcttgat ctaaaataga catacggatg gaatcgtact gcccaactgt cttcacatca
    17041 acattcacta ccgcctgagt caaatcttga tttagctccg tccgaataac aatatcctca
    17101 atcctatttt ggggacgttt atagagatga actgtccgct ggataccagc atagttgaaa
    17161 aagtcaaaat tttccttgac ggtcttcaca atttgaccat tctcatccac ttcttcgcta
    17221 taattcccta ctgggagggt tgtgtaattc aactcattat tagcacaaac cgtcaaacgg
    17281 aattgatcag cgtcgtataa gtttgacgga atcaagcatt caaaaggtgt gaaaccaccc
    17341 ttgtgctccc cgattaactg accatctgca tagactttgg cctgatgtgt caccgaacca
    17401 aagcgcacaa ccaactcttc atcagctgat acgacaggga gagctaccat aagttcatac
    17461 caattatctc cgacaaaacg acgtttgtcc ttatcaacaa caagatcatt aaaagaactc
    17521 ggtacaacca ttacttcatc tgtatctaac agagtatgaa catcattgtt cccttgctta
    17581 aatttccata ttccatttaa agaataaact gagcgtgttt ttgtctgtat tggatacaac
    17641 atactttttc cttctttcta tcgttttatt tcacgtgacc cttttttcaa tagcaaatca
    17701 atatctgatt gactgatgac attaatgtcc ccttcaatgg tatgtttgaa ttgaaaacta
    17761 gccataccta agtcgagtat ttcccgtgga gatttttcct gcaaatagcc caaaataatt
    17821 cctgctgtaa aggcatcccc tgtcccaact cggtccagaa cttgaatgga ttctttttcc
    17881 gtttcataaa gaataccatc ttgatacaaa tatgctttca gcaaatattc attattgtag
    17941 gtcatttccc tctgggtaaa agcaagaccc tgcaaagaat aagtctccgc cagaccttta
    18001 accaccgcta acaagacttc tttattttca taagggcgct tcaaccccaa ttcatccttc
    18061 agatcttttc cagattcatt gaagagttga ataggctcta gaccaaagca aatatctgct
    18121 aaacctacaa aaggagataa tttctccctc gcttcttcaa aagaagacca caaactttct
    18181 cgataattca agtcaaagga aaccttaaca ccctgaccct ttgccatttc catcaaacga
    18241 aaagccagtc gataaatatc tggattcaaa gcgaccgtta taccactgac atgaaaccaa
    18301 tcgacaccgt caaacatgcc ttccaaatca taatcttctt ccttggataa gctgaaagca
    18361 gaataatcac ggtcgtaggt cacctgactg ggcctgagcg aaaaaccttt ttgatagtaa
    18421 taaagtccaa gccttccctc gtttcgaatc acatatttct gtccaatctg ccgtgcaaag
    18481 agaaagtgtt ctgccatttt tcccacttca ttcttaggca gtgccgtcac caactcaact
    18541 gaatgcccca gctgagccaa gctggctaaa acattcaact ccgaaccacc aaactgacaa
    18601 tctaaactgg tagcctgtgt caaagtttga tactgcggag gagatagccg caataaaact
    18661 tctccaaatg ctaagatttt tttcataccg cctcctagat gaaagaccca gctggactcc
    18721 tccaactagg tcgttattgt attatttatc gtcgtattga tcaaccttat attgagttac
    18781 atcaacataa tccatatcag ataatggaac atttttcgcg attgcacgca cacgttcctc
    18841 atcttttgcc gctttcataa catggatttt ctcttgaata cgtgccattt cttcacggtc
    18901 tagtttgtag aatttcatca agaacaagga tacgctcaat accgcggctg gaatcactgt
    18961 caataggaca atcagtgcca ttctcaaatc tgtagagaat gccgtttcaa cggttgggta
    19021 ctctttacca aaaccgatac ctgctaatac aaatccaaca accattgggg caaatgaaga
    19081 agcaattgag tctgtcaatg agaagatagt cccaatcata cctgacaagt aacgaccaga
    19141 tactgaagtt tcatagtctg taatatccgc acccatggtc aataccaaac cagatggagc
    19201 actagatgcg tagcgagcaa cgatataaac aacaaagaag ccgattgtgt agagattcca
    19261 atttgtcaaa tccaacatac ctggtttagc tgaaaagagc atcgctgcca agatcacaag
    19321 accagcaatc ccaagttgaa gcgctgtaat ataggcaaaa cgtaaacctt tacgacgagc
    19381 caattgcgca gcaccaactg ttacaagaat acctggcaaa atcaaaagca tagacatttt
    19441 tccagaaaga gcatagtcac cgaagataat accatacagc ataaccccga taacggaatc
    19501 tccaaacagt tgagcaacaa attttacaaa agctgcagag attgaaagaa cctgcaaagg
    19561 tttgtttcct ttgataactt tccagtagtc tttcaactta gtttcttgtg tcttctcacc
    19621 aagaccaaag tatttcttat tgtctttctc ccaaataccg ataatagcaa gaatacccag
    19681 aacagctgaa attactacta caccgaggaa caattcctgg aagaaacttg gagtgaaacc
    19741 accatgtttc ggaaccaatg tccctgacac aacaacctga ctaccagtca tcaacacagt
    19801 tgtcgcaata ccatcaacaa tattaaagat tggacgttgc tttggatcat tggtaagcgc
    19861 aacttgtcct gcttttgtaa tcgtctgttg aagtgaatat ccaattttat ggataatcaa
    19921 aacaaggatg aatagaggaa gacggagact ctcattaact ccacttaaac ctaacatcat
    19981 aaaaagagaa gctgctgtaa tgatattccc caaaaccaag ataggacgat atttaccaaa
    20041 cttagtatcc gttttatcaa tgataactcc gattgtagga tcaatgaaac catcaaagat
    20101 acgaatatag cccatgagtt gactaacaaa cagggctgca agaccaagca cacctgtcga
    20161 taaataagtc acgaaagtaa aagcaaccaa gtaaatattc gtcgatgaat tgttcatcgc
    20221 aaataatatg atttgccata acttggcttt gttataagta acgtcttcga cacgttgctc
    20281 aacatgtttt tccattggaa attcctccta aatgttttct acgacaaagt cgtaagcgtt
    20341 ttctttaact atattctaat attcatcaga tgatttgtca actgtttttg taaatatttt
    20401 taattatacg caaaaaaaga cgatttcacg tgaaaatcgt ctgatatatt tgatttagcg
    20461 aggaagaatt catctgacta tttattgatt tactgcaatt tccccactat gatagttgat
    20521 actgagacca ctctgcacat ttggaacgaa cacattgaac tctaggctac catctgctgc
    20581 ttttgcctct agatggcttc cgacaggaac aaggtcttca ctctcagcat acaccaaggt
    20641 cacacgatag cggatacgct tatttttatc caaggcccgc cgaatctgac tttcaaaata
    20701 attctgccca gttgaagttt ggtcgttagc ttggttggac caagctgttt gaactgcaat
    20761 attcttagga ttgctggttg aagcatcgaa gccagacaat ccaccaatca aagcataacc
    20821 taggaggtga ccacggtcca ctgcatggtc atattctcca gttaaatcat ggatctgatg
    20881 ccaacccgca ggtgtccaag aagttcgacc atttcctgtt tcttcacgat ccttatactg
    20941 acgtgtagat ttagctaaaa gcgcattggc tactgttgga accgtttctc cctggactgt
    21001 ctttgtctta ttatcaccat aaggtacact ggcaaccgat gcatctaaat ctgtttgatt
    21061 accgttaata ataaatgctc cagcgccatt ccattcaatc gaattcccca gttgcttttt
    21121 tacatcttct gtcaataccg aactagccag ttcctgacta ggtgtgcctt cacttgcctt
    21181 agactgattg acataatagg aataatcagc aacgttactg ctcttgttat cacttaaaag
    21241 atagccaccc gctacgacaa caatcgtagc caataagctg agcagactca ttgcttgttt
    21301 tcttacattt ttcttcgcca ttttctctcc atcaaaaaag caggtccagc ctactttcct
    21361 gtaaatttac cgattagttc ttgccactta gctggtgaca acaccgcaaa tacattgtct
    21421 ccatctccta tgacaccgta accaaccatt aatcctaaaa caaacaccaa aatagcaatc
    21481 aacaaaacaa ctgctactaa gattaaattt ttaccaacat agctaagatt atccttatcc
    21541 attttcatct tctccactca cacgttcaat cgttgcacct aaagcagcta atttctcatg
    21601 aaaacgatag taacctcgat ctaagtgcgt taatttacct acaatcgttt caccttcagc
    21661 aaccaaacca gccagaatca aagcggcact tgctcgtaaa tcagtagaca taacctgagc
    21721 accttgcaga gcaagtcctc ccgtgatgat agctgtatct cgtaaaattt ccgaatgcaa
    21781 atccatccga cgcatttctt ccaaatgctg gaaacgattt tcaaataccg tttcaatcat
    21841 ggtagattca ccctttgcaa ctgccattag agctgtaaat tgagcctgca tatccgttgg
    21901 gaatcctgga tggggcaagg tttttactgt tacaggttta agcttatcca cttgcgaacg
    21961 aactcgaatc ccttcagctt cttccgtcac ctcaactccc atttccaaca tcttagaaat
    22021 gagtggacgg ttatgctccc agacagcgtc cttgaccaag acatctccac cagtcattgc
    22081 cgctgccacc ataaaggtac ccgcttcgat acgatcttgc accacttgat gttctgcgcc
    22141 agtcaattct tctacaccag taatcgtaat cgtctctgta cctgccccct taacttttgc
    22201 ccccattttg tttaggaaaa tagccaagtc aacaatttca ggttctcgcg ctgcattttc
    22261 taataccgtt gtaccctgcg ccagagttgc agccatcatc aaattttgcg ttgccccaac
    22321 accaacactt gggaaatcaa gatagatatg agctccctgc aagttctctg cactcgcttc
    22381 aatataacca gctcgttgtt taattttagc ccccatagct tgcaaacctt ttaagtgcaa
    22441 atcaattgga cggctaccaa ttgtacaacc tcctggcata gaaaccttag cataaccatt
    22501 gcgtgccaaa atcggaccaa gaataacaat agaggcgcgc atttgactca catacttata
    22561 aggagtttcg ctaccaagtt tacctgttgc gtcaatatcg atagtctttg tttcctcgtc
    22621 aaatttcaca cgaactccca gaccacgtac aacattattc attgtataga catcagacaa
    22681 aataggaacc tgagtcaatt tggtttgccc ttgacttgct agaactgttg ctgccaataa
    22741 aggcagaacg gcattcttcg ccccctcaat ctcaacctgt cccttcaaac cgtttttact
    22801 acctgtaaca ataattgtat ccatttctta tccttttctt atcttaaatc acatatttgc
    22861 aaaagagcgt cccaccatca acaattctaa aaagaaagtc gagactaagt accccagtgc
    22921 tattgccaga aagagaatca gcaaattgat tttacgctga ttatcccccg tcaccttcaa
    22981 ccaccgtgac caatcaactg ttgtaaccaa gagatgatga gccagataga taaacaatat
    23041 atgactaaaa aactgtaaaa ttgaaattac catgtttcca ttataccatg aaattaaaaa
    23101 atggaactga accaagttca atcccatata gattaatgtt tcccaacacg aatacgattt
    23161 atcgcacgtt ggagggcgac tgttgcacgc atctccatat caatattacg agcagtctgt
    23221 gcttcttcca attcacgttc cgcgcgcaat ttggcacgtt ctgcacgact aatatcaata
    23281 tcccgttccc gctcagcgga atcagaaatg accgtaataa tatccttgtt gacttcaatg
    23341 attccaccat tgaccgcaat ccagtctaca tggttttcat catcaacacg acgcactttc
    23401 atttcaccga tttccaaaac tgcaatcaat tcttcatgtc cagaatagac acccatctca
    23461 ccatcaaccg ttttgaccaa aacaaatgct gcatgatggt catatttgat accgtctggt
    23521 gttacgattt gtacagtcat ttgtcccata agccacctct agaatttcat tttagcagct
    23581 ttagcgacca catcctcaat tgaaccaaca tttcggaagg catcttctgg tagatggtcg
    23641 tgtttgccgt ccaagatttc cttaaagcct ttcaccgttt cagctactgg cacataagaa
    23701 cctggcatac ctgtaaattg ctccgcaacg ttgaagtttt gagagaggaa gaattggata
    23761 cgacgagcgc gaccaaccaa ggtcttctct tcatctgata attcatccat accgagaatg
    23821 gcaataatat cttgcaattc ttggtaacgt tgaaggacac gttttacttc catagctact
    23881 gcatagtgct cttcaccaac gatttgtgga gcaagagcac gtgatgacga agccaatgga
    23941 tccaccgcag ggtagatacc aagctgagtc aacttacgct ccaagttagt tgttgaatcc
    24001 aagtgagcga aggctgtcgc tggagctggg tctgtatagt catccgcagg tacgtaaata
    24061 gcctgaatag atgtaacaga acccttcttg gttgaggtaa tacgctcctg caattgtccc
    24121 atctcagttg caagtgttgg ctgataacca acggctgatg gcatacgacc caagagggca
    24181 gacacttctg aaccagcctg tgtgaaacgg aagatattat cgatgaacag aagaacatcc
    24241 tgcccttcca catcacggaa gtattccgca atggtcaaac cagtaagagc aacacgcata
    24301 cgggctcctg gtggctcatt catctgacca aataccatgg ccgttttttc aataacacct
    24361 gattctttca tttcccagta aagatcgttc ccttcacggg tacgctcgcc aactccggta
    24421 aatacagaga taccaccgtg ttcttgtgca atattgtgaa tcaattcttg gataaggacg
    24481 gttttaccaa caccggcacc accgaagaga ccaaccttac caccttttag ataaggtgct
    24541 aggaggtcga taaccttaat ccctgtttcc aaaatttcgc ttgaagtaga taattcgtca
    24601 aaagccggcg ctttcttatg gataggctca cgttcaaaat ctgccggaaa agactcttca
    24661 aggtcaatgg tatctcccaa cacattgaag acacgcccca gcgtttcttt accgactgga
    24721 acagagatgg gacgacctgt atcgagaact tccatcccac gtgtcaatcc atccgttgat
    24781 tccatggcaa tggtccgtac aacgccatca ccaagttcca aagccacttc aagcacgact
    24841 ttttgtttgg aatcatcatt tttatataca acgagtgcgt tgttaatctc aggaagttta
    24901 tcttctgctg caaacgcaac gtctacaact ggtccgacaa cctgagtaat tttgcctgaa
    24961 ctcattctat ttcctctatt tctattactc aagggctgag gcaccagcta caatctcagt
    25021 aatttcctga gtaatcgctg cctgacgagc cctattgtat tggattgtca aatcgttaat
    25081 gacattctta gcattatccg tcgctgtctg catggctgtc atacctgctg cattttcagc
    25141 agttttggca tccaaaatcg ctccataaat agttgattct gcatattgag tcaagagttg
    25201 ttccaaaata acctcacggt ttggttccaa ctcaaaggta gctgtataac catctgcttc
    25261 attgtggtcc aaatcttcta caggaagcat ctgttgtaca cgcacctgac tggtcaaact
    25321 attgacatgg tggttataac aaacatacaa ctcatcaaag agttcattct tgtacatttc
    25381 aacagactta gaaatgattt tttgaacctc ttcaaaactt ggattgtctg ctaaaccacg
    25441 caattcaaag acaggattga taccacgagc acggaagaaa tcagctccca tactaccaat
    25501 ggcaataatc tcatactcat cttttgagtc atggtcctgt tcaatcatcc ccatcacagc
    25561 cttcaaaata ctggaattat aagaaccttt tagtccactg tcagaggtaa tgacaatata
    25621 accagatttt tgaactggac gacgaatcag cattggattg cttgtatctg aagccatcaa
    25681 ttctccacgc aagagatccg ttgtaatctg acgaaccttg ctagcgtaga tttgaaagga
    25741 ttgcgctaat tgctctgatt tggctagttt agaagccgaa accatctgca tggcaccggt
    25801 gatttgactg gttttcttag tagatgcaat ttttgacttg atttcattga gagaacctgc
    25861 cattattcac tcctttactt aaagacagac tggtctttga aagcttggat agcagcattc
    25921 aattcatctg tatcaggaag atcttttgtt gtacgaataa catccaaaag accatcgtag
    25981 tgcaaatcaa aatatgagta cagttcttct tcaaatgcca aaatatcgtc aattggcact
    26041 gaatccaaga agccatttgt caaagcatac aaaatcaaaa cctgtttttc aactggaagt
    26101 ggcttatgca atggttgttt caacacttcc acagtccgac gaccacggtt gagtttggcc
    26161 tgagttgctg catccaaatc cgaaccaaat tgcgtaaagg cttctaattc acgataagaa
    26221 gctaagtcga tacgaagggt acctgccacc tttttcatgg ccttaatctg tgcagagcca
    26281 cctacacgtg atacagaaga acccgcatca atggctggac ggataccaga gttgaataaa
    26341 tcgtccttca agaagatttg tccatccgtg atagaaatca cgttggtcgc gatgtaagct
    26401 gaaatatctc ccgcttgtgt ttcgatgaat ggtagagctg taatggagcc accgcccaat
    26461 tcatctgaaa cctttgctga acgctctagc aaacgactgt gcaagtagaa tacatcccct
    26521 ggataggctt cacgacccgg aggacgacga agcaagaggg ataattcacg gtaagctacc
    26581 gcttgttttg acaaatcatc gtaaacaatc aagacgtgtt tgccttcata catgaactct
    26641 tctgccatcg caacaccagc ataaggagcc aagaaaagca atggagacgg ttgtgaagca
    26701 gatgccgtta ctacgattgt gtaatccaaa gcaccatatt gacgaagggt ttcaacctga
    26761 gtacgaacag ttgactcttt ctgaccgata gccacataga tacaaatcat gtcttggcct
    26821 ttttggttga gaatagcatc aatagctaca gatgtcttcc ctgtctgacg gtcaccgata
    26881 atcaattcac gctgaccacg accaatcgga accaaagcgt caatagcctt aagaccagtt
    26941 tgaagcggtt cattaaccga cttacgttgc ataacacctg gtgctggata ttcaatcgga
    27001 cgagtcttac tagtacgaat ttcaccaaga ccatccaccg gttgaccaag tgggttgatg
    27061 acacgaccaa tcaaagccga accaactggt acttccataa ttttaccagt acgacgaaca
    27121 actgaccctt cacgaatatc agtaaattgt ccgagaatga taataccaac atcgttggtt
    27181 tctaagtttt gtgccatacc aatggtgcca ttttcaaata ccaaaagctc tccactcatg
    27241 gcattgtcaa gaccttgcgc acgggcgata ccgtcaccga tataggttac gacaccagtc
    27301 tctgtgtagt caaagtcagg ctgaaagcct tcaatttgtt gttttagtaa agcgctaatt
    27361 tcttgtgcat taatcaaaag aacaccactt tctattttct aaagtttttt tctaacgtcc
    27421 ttcaactgtg tccgaacgct tgcatcgatt accttatggt taaccgttac aataaaacca
    27481 ccgaggagac tttgatcaag ttcttccgtg atattgcgca cacgcaagga aaatcgttgt
    27541 tctaccaagt gacggagacg ctctttttga gcttctgtca acggatagac tgaaaccaca
    27601 cgggcttcaa attcattttt aaatttactg atttgtaatt ggacacgctc aagggtttca
    27661 agaagcaagt ctgcatgacc atcacgaata acatcttcaa tcaagtcatt gatttgccag
    27721 aaactagatt gacgtaccgt ccttacaaag tcagcctttt cttctctcga tactgtcgct
    27781 gacagcaaga ttcgattcag cttactatca tgaatgatgg aaatcaagtc agaaatctgg
    27841 tcatacatat cccaaatatc tgcatgatca ctgacttttt caacaaatga tagagcatat
    27901 ttttgcacga tggcattttc tctagcgttc atctacttgt ctcctagttt gtccaaataa
    27961 cggtcaatca agttactatg agcttgttgg tccaaatctt ccaaaataat tttgcttgca
    28021 agatcaactg ccaattcagc aacttgaaca cgcaagtttt cttgtgcttc acgtttttca
    28081 gcttcaattt ccaattgagc tttttgcttc aatccttgaa tttcaacatc tgcttgctca
    28141 agaatgcgtt ttttctctag ttttgcgcgt tcaaccgcgt cttgaataat cttttgactt
    28201 tcaatacgtc cctgtaccaa ctcatcttca cgttgttgaa caaggtttgc tgctgctgtt
    28261 aatttttctt ctgcagcatc aatatcgttc gcaatcttat tggcacgttc ttcaaaaata
    28321 cctgtaattt tatcccatgc aaaaacacga ataagaataa taagtactgc aaatgaggct
    28381 gttaccaaga taaagtttcc aagaatggta ctagattgca ttgtaatagt tgttgccata
    28441 gttgtctttc cctttcctat tcactctcac tactgatttt tttatttaag tacatagata
    28501 ccaacatgat aaatacatat ccctgcaagc atgaaatgaa tacagaaaat gctgtccaaa
    28561 caacgttcag agcaaaggca attggatatg caagtgcact ttgttgagat aattgtagaa
    28621 gaagagagac aagcacttca ccagcataaa tgttaccgta cagacgaaga gcaagcgaca
    28681 caaggttggt cacttcttcc aaaatgttca ttggagccat agcccagggt gtcacaaaag
    28741 atttgaggta tgccttgaaa ccgcgacgac gaataccctc aacatgacaa aatactgttg
    28801 caattgttgc caaaccgaaa tcgtaagcca tattggccgt tggagaagtc cacaaattat
    28861 gtccttctgc tgtttccaat ttggtcatca aacctagatt attggcaacc aaaagaaaag
    28921 taaagactgt aaagaagaac aaggaatacc gctttgcttc cgcatcgcca agattccctt
    28981 ttgtaaaatt gatggtcaat tcgtaaacgt actccaaaac attctgtttt ccttttggct
    29041 tgagctccat ccgacgacta gcccaaaaaa caagtaagaa aataatggaa atggtaatga
    29101 cagaaaccaa taccatggtc aggtcaaagg ttacgggacc aagggtaagg gttggactta
    29161 aatgttcttc caatggaggt tccccccttt tctatttctc tattttctac tatttcaaga
    29221 caaatgtcat agccaaggtt acgaagaaag ttccctcgat aaaggcaaca cccaaaaata
    29281 caccagccat caatttactt tgcatttctg gttgacgtgc agtcgaacta aggtatgaag
    29341 ccaccaacag tccctcaccg atacttacac caagacaggc caatccaagg gccaatgcgc
    29401 ctaaactcat aacgaattct ccttttaaaa tttaatacca tgtaaggata ctcctaaaac
    29461 atctatttgt caagaaaata ccgccattgt aagcgtttaa ttcgtttttg taaaataaaa
    29521 agaaaagagt atttcatctt ttcttcctat ttaatagctt catcaatatg gtctgtttct
    29581 tcaatgaatc gtttggccat atcttctcga ttcatctgat cagtatcaag agtgattgca
    29641 tggtcagaaa ccagatctgt atctgcgaaa aactctatat ggtttgatag gttgtgcaga
    29701 cgtacagggg cttccccacc aaactctcca tctagattga gcagtagacg gttatctggg
    29761 ttcagatttt cgatggatag gctcttggtt tttatgtatt caactaaatc actctcaatg
    29821 tgtttgccac cgtctaagac ttgacgaatc agatgcagaa tttcaaatag attccctgtt
    29881 ttaaccatca ataaggtaaa attaccatca tctaacttag catcgggaac aatctgttca
    29941 aaacctccaa ttgaattggt taaggcgaca aaaatcattg aaaccgagcc ttcaaatact
    30001 ccctcgtcat gctctacacg aacaggtgta aattgtactt gtggcaggag ttctgctcct
    30061 tttactacat aggccaaata gccaaacatg gtcttaagtt gactgggaac actgtaggtc
    30121 aactccgtaa gtgtacctgc cgcagcaata ttgataaaat aatgttcttg atgaaaacca
    30181 tttttttgat ttttggctaa accaatatcc attaaaatgg tctgctgctt accgataacc
    30241 ttagctgctt ctacaggatt tcccctaggc acttttaagg cacgcgcata gtcattggtg
    30301 gtgccagtcg gtatgatggc catctgcgga cgtttttcaa gcggagcaat tccattgacg
    30361 acttcattga ttgttccatc tccaccagcc gcgataatca agtcaaagtc tgcaagcgcc
    30421 gcacgtgtag cttcattctt tgcagagtct ttctcagcag ttgtttggaa agctgaagtt
    30481 tcataaccgt agccttccaa aatttccaat acttcggcca catttttctt catgatttct
    30541 tgacctgagg tcggattata tattagtcta gcacgttttc tttcttccat cgttttacct
    30601 ttacaagctc tctaaccaag cctcatcttt aatttcgata cctagctcct gtgctttggt
    30661 caatttactg cctgcatctg ttccggcaac cacaagatta gtttttttag aaacagatcc
    30721 cgccacattt gcccctaaag cttctaattt ggctttggct tcgtttcgtt tcattcgctc
    30781 caatttacca gtcagtacaa ctgtcatacc cgatagcgca gcattttcat ctactacttg
    30841 tccgagatag gttaagttta ccttagcttc cttaagttcg gctaaaagtt gttgcgcacc
    30901 ctcactagca aaaaaagttg tcaaagactt agcaataacc tgaccaagac cttctagcga
    30961 agcaattgct tcttgatcag caaatgctag agtttctaaa tcaccaaatt tttctactaa
    31021 aatcttactt gccttactac ctacatggcg aatacctagt ccaaatagga ggcattctgc
    31081 tgagttactc ttagaagttt gaatggcttg atagagcttg tcagctgatt tttctttgaa
    31141 accttccaag gtcaataggt catcaacagt taatttataa atatcagcaa catcgtgtac
    31201 caagcctgct ccaaagagtt tttcaacgat tgaagaccct agccctgcga tattcatagc
    31261 atcccgactt gcaaaatgtt ccaacttact catcagctgg ctaggacaaa tagggttgat
    31321 acagcggagg gcaacttcgt cctcatagtg ttgtaggtcg ctctgacaag acggacaatg
    31381 actaggaatg ggcatgattt cttgctcttt tcgatacttg tccaccactt tcagaactgc
    31441 tggaataata tctcccgcct tataaacaat gaccgtatcg ccaatacgaa tatctttttc
    31501 ggcgatataa tctacgttat gcaaagttgc tcgactgaca gtcgttcctg ccagttgaac
    31561 aggactaaga ttggccgttg gtgtcacaac acctgttcgg ccaacagtcc aatcaaccga
    31621 caagatttct gcttcttttt cctcagctgg aaacttgtaa gctactgccc atcgtggtgc
    31681 tttaactgta aatcctagtt cttcttgtat agctaaatca tttaccttga tgacaacacc
    31741 atctatctca tagggtaaat catctctacg ctctgctatt ttttcaataa aatcccacgt
    31801 gtcatcaatc gactccgcca agcaatattc gtggttggtc acaaatccca gagcatccag
    31861 tttctccagt acttgtgact gactagtcgc ttcagatgga ctagcctctt ggtagaggaa
    31921 agtagcgaga ccacgctggg ccaccactcc cgtatcaagc tggcggaggg tccctgctgc
    31981 tgcattacgc ggattagcaa attcagcttc accagcctcc tgacgctgtt tgttaacacg
    32041 gtcaaaggaa gcttttggca tataacactc acctcggaca gtgatgtcca cagcttcggg
    32101 taaggtcaaa ggaacatcag ccactcgttt gagattttct gtgatatttt caccgacact
    32161 accgtcacca cgtgtagcac ccacgactaa attacccgct tcataggtca gggaaataga
    32221 cagaccatca atcttcaatt cacagatata cgttgcttga ggaaattcct tgcgaactcg
    32281 ctggtcaaaa gcttctaact cttcacgaga aaaggcatcc tgcaaactaa aaagaggata
    32341 aacatgacta tatttttcaa atccgtctag catctttccc ccgacccgat gggtaggact
    32401 atctggtaaa atcagttctg gatgagccgt ttctagctct actaattcac gataaagcgt
    32461 atcatattct gcatctgata cgctaggttg gtccaattga taatactctt tagcgtattg
    32521 attaagcacg ctaactaatt ctgatattct tgttttcata agaatattat atcatgattt
    32581 gagg
//
LOCUS       DBNFEG010000006        32525 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.006, whole genome shotgun sequence.
ACCESSION   DBNFEG010000006 DBNFEG010000000
VERSION     DBNFEG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 32525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32525
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.006"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(93..701)
                     /locus_tag="AC1PNG_000160"
     CDS             complement(93..701)
                     /locus_tag="AC1PNG_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HIP6700703.1"
                     /translation="MSYQMIKKQFKLGLFTALIGNLLWLGMVNVSADDTVISDSPTVS
                     ALTKKVEAAKIAAKAAAEKIVAEKAAAAAKAALATNMVSEVAVEYTANTYPAGQCTWG
                     AKEMAPWVGNYWGNGGDWAASAAALGYEVGTTPKVGAIAVWTDGGYGHVAYVTDVAAN
                     GHIQVKESNYGGVYYPSNVRGFFDPTTTSEGTVSYIYPPAGV"
     gene            complement(1034..2410)
                     /locus_tag="AC1PNG_000161"
     CDS             complement(1034..2410)
                     /locus_tag="AC1PNG_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIP6700704.1"
                     /translation="MSNISEKFMEISGKIGSQRHLIAIRDSFISMMPITMAGTVAVLL
                     NVFLRDIPNNMGWTGFADAMQPVIDINGYVYFGTIGIMALVFAFTFGYNLSLMYKVNP
                     LAGGLISFASFISTIPQSVTISTTLDGVDKSVVSALEGLGLSIVTTDGVSALETSQWG
                     AIALKYGGATGLFTALFIGFLATWVYASLVKRNVIIKLPDSVPPAVNKAFAAIIPGTA
                     AIYASSIVAYLAFTLTGQSLGDLVSTYIQLPLLGLSQGLGSVILLTFLVQLFWFFGLH
                     GHNVLAPIMDGVYMVALNENAAAYTATHSTANLPWLWTRGSFDAYAQMGGSGVTLALI
                     IAIFIFSKREEYKMVAKLSAPMGVFNINEPVTFGIPMVLNPLFAIPWLIVPPICATIA
                     YLATAAGIIPPVFAPVPWITPPGLYAYLATGGNIMAALVSLFNLFVAFLIWTPFVIAA
                     NKVKAGEE"
     gene            complement(2454..2894)
                     /locus_tag="AC1PNG_000162"
     CDS             complement(2454..2894)
                     /locus_tag="AC1PNG_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HIP6700705.1"
                     /translation="MKISKIGQVPIERLFATIRQSLKDDFQQNTGSILQDQDIIQGLT
                     YTKTFGDKGQHSVKVTVEEFTAPYCYAVCFHSNRGKEHVRYQLEDLGNDQTNIEYSYE
                     MEAGDIFVKGNQFLMEKIFSKSIKRQTNAQLEALIRCSVEPVSV"
     gene            complement(2913..3227)
                     /locus_tag="AC1PNG_000163"
     CDS             complement(2913..3227)
                     /locus_tag="AC1PNG_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIP6700706.1"
                     /translation="MNSPEYLEAIMGLIMYGGEAKGFASQAIQAAKEGRIDEAEQLLQ
                     DAIASLNIAHKSQTQLLAKEASGESVELSLLMVHGQDHLMNALTFIDLAKEFVDLYKQ
                     IG"
     gene            complement(3229..3540)
                     /locus_tag="AC1PNG_000164"
     CDS             complement(3229..3540)
                     /locus_tag="AC1PNG_000164"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIP6700707.1"
                     /translation="MKHIMLVCNAGMSTSMLVTKMQKASEETGVEATIWAVPVSEADN
                     EVAQKEIDVLLLGPQVKFLLNDYKAKFEPTIKVDAINMMDYGVMNGAKVLEAALKMME
                     G"
     gene            complement(3553..5547)
                     /locus_tag="AC1PNG_000165"
     CDS             complement(3553..5547)
                     /locus_tag="AC1PNG_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIP6700708.1"
                     /translation="MLSKKEVRLLEALLAKQETFVPSIELAKVLSVSDRTVRTYIHQL
                     TSSLENSGAHILSKQGSGYCLQIERPVEFEMFWQEQLLLKKRLTDLTQLEEAGDRQRY
                     ILNKLLFEDPVQSPECLQRELFIGKTTLHSILADIRKLFIPYQLELLVTRKGIELKGD
                     EPAIRHFIMDYFFDEGNVQSVYGVVETNLLPDIRFTDLMLIVLDECRDAKLRLSDFVM
                     QNLVLHIALLLQRLKIGSGLDRFPIPERIQSSQEYLVAQRIIERIEEMFQVTIPSEEA
                     NYIALHLKVKLTGNPNQDGEVEEIFRQNVENSLLHLSHLTGLELSKDQQLFKGVLAHL
                     MPLRIRLENNIQLGNPLTAEIKDQYFEAFELTKTAFAAIPELMGYSISDDEWAYLTLH
                     VMAAIERYQGFKKTRVLVVCATGIGSALMLKNRLEKEFSSSLEILDTVSYYEVTEERL
                     KDVDLIISSISLSNLIFMTPVINVSVFLSEKDIEAIRSHLQQTTPVIPDQGESVLMEV
                     GQAKQIAEAVFAPHRIIYLEEDCDREVALKQLVTTLNESIEPDFVNRFIEQVNLRESY
                     SPVVFGDWLAFPHPALPMSYSEQIVVGIYKNAVDWGHGKQVQFVFLLSPSKGRNAFLK
                     YISPCLVDFVQDRVLQERLLHHPNYKELIDIFIPLIQRKG"
     gene            complement(5639..6745)
                     /locus_tag="AC1PNG_000166"
     CDS             complement(5639..6745)
                     /locus_tag="AC1PNG_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HIP6700709.1"
                     /translation="MVELGFSIYPENYSLEESQAYIKLLHQYGARRLFMSLLQLENGD
                     RTVFELYQKLVDYANQLGMTVIADISPHFIAAQGWDGALMERAAELGLAGIRLDEALP
                     LEEIIEMTHNPHNIKVELNLSTDRTLLSNLLASVANRDNIIACHNFYPHAYTGLSQEH
                     FLEMSSFYYQSGIRTAAFVSAQTASEGPWPLSEGLPTLEDHRFWLLPLQIAWMKATGF
                     IDSILISNQFISEEELQSIQTVLNEDTISLMVDIEEGLTPVEREIVAFDHVYRGDISA
                     YVLRSTMPRVVYKDASVPARSDQAIPVGRGDILIDNDLYGRYKGELQIALKEFTISPK
                     VNKVGRISPDYLPLLRFIKPWQEFKLVIASEHFH"
     gene            complement(6755..8155)
                     /locus_tag="AC1PNG_000167"
     CDS             complement(6755..8155)
                     /locus_tag="AC1PNG_000167"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIP6700710.1"
                     /translation="MTKDKVFYQFPTNFLWGSSTSGPQSEGIEPGDGKGQNNWDYWYS
                     IEPERFHNKIGPSQTSTFYKHYEKDIDLLVETGHTVFRTSIQWSRLIPTGTGEINQEA
                     VSFYRSVFSLVRAKGIQLFVNLYHFDLPYCLQEKGGWENKEIVRAYEAYARTCFELFD
                     DLVDRWITFNEPIVPVECGYLGNYHYPCKVDAKAAVAVAYHTQLASSLAVKACHEVNP
                     HKKIAIVLNLTPAYPRSEQAEDIKAARIAELFQTKSFLDPSVLGEYPDELVELIDAYG
                     LMPEVSDEELAIIKENRVDFLGVNYYQPLRVQAPTNSWKEGEPLTPEHFFAPYDMPGK
                     KMNPHRGWEIYEKGIYDIAINIKENYHNIEWFVTENGMGVEGEGAFKDNGLIQDDYRI
                     EFIEDHLIELHRAIQEGANCKGYMLWTFIDCWSWLNAYKNRYGLVELDLDTQERIVKK
                     SGKWFKKLSDANGFHR"
     gene            complement(8152..9513)
                     /locus_tag="AC1PNG_000168"
     CDS             complement(8152..9513)
                     /locus_tag="AC1PNG_000168"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HIP6700711.1"
                     /translation="MLKTLKLVTIGGGSSYTPELVEGMILRAHQLPIREWWFVDVEQG
                     AKKQEIVVELARRMVAKAGLDWKIESTLDRREALIEADFVTSQFRVGQLPARYLDETI
                     PLSYGMLGQETNGAGGIFKAFRTIEAYKGIIKDMKDLCPNAWLINFTNPAGIVTEAIA
                     NELHWKKVIGLCNIPIGQKKLAAQILGQSEGDLSTRHIGLNHFHFHEVWDKYGENRTQ
                     VILETLYGDLREETPEAVKNITNLEFPIQLLRTIGMLPCDYHRYYFLEQEMLEDALEQ
                     YRQGNVRAKVVQEVETELFELYKDPQLKDKPKQLELRGGAYYSDVACQIICAIHNDSR
                     EELTVSTVNRGVIPYLPENCVVEVTSIISAQGATPLACPPTPPIVTGYLQLMKQMELV
                     TVQAAMTGDYSLALQAFTLNPLISNGPQAEVLLQDMLLAHENYLPQFAPAIQHIKERR
                     NNQ"
     gene            complement(9782..12283)
                     /gene="leuS"
                     /locus_tag="AC1PNG_000169"
     CDS             complement(9782..12283)
                     /gene="leuS"
                     /locus_tag="AC1PNG_000169"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIP6700712.1"
                     /translation="MSFYNHKEIEPKWQGFWAKNHTFKTGTDAAKPNFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPADFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKIKDTDKDFTVFTTRPDTLFGATYAVLAPEHDLVDSITSPEQ
                     TEAVAEYKRQASLKSDLARTDLAKDKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFGLDIIPVLEGGNVEEAPYTEDGAHINSDFLDGLNKEEA
                     IAKMVAWLEENGVGQEKISYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPENELPLV
                     LPKTSDIKPSGTGESPLANLTDWLEVVREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NDEKLADEDLLKAWLPVDIYIGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRNSRGALVATDKVEKRDGSFFNIETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQFGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLLTTKELAAENSGALD
                     KVYNETVKTVTEHIEDLKFNTAIAQLMIFVNAANKEDKLYVEYAKGFVQLIAPFAPHL
                     AEELWQGLTNTGQSISYVAWPTYDESKLVESEVEIVVQIKGKVKARLTVAKDLAPAEL
                     EKVALADEKVQAEIAGQTVVKVITVPNKLVNIVTK"
     gene            complement(12386..14278)
                     /locus_tag="AC1PNG_000170"
     CDS             complement(12386..14278)
                     /locus_tag="AC1PNG_000170"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HIP6700713.1"
                     /translation="MTRLQDDFYEYVNGEWAKTAVIPDDKPRTGGFSDLADEIEKLMI
                     DTTNAWLAGEDVPEDSVLQNFVAFHKQVADYETRDRLGAEPAQALIAEYKALNSFEEF
                     TSKLAEYELAGKPNLMPFGVAPDFMDATTNVLWADSLGIILPDTTYYEEGHEKGAELL
                     KIWRESQEALLPKFGFSDEEIKDLLDKRLELDATIAKYVLSNEEGSEYAKLYHPYEWA
                     DFTALAPELPLDDFFTAILGQTPDKIIVPEERFWQAAKDIYSADNWELLKATLILKAA
                     GAYTAFLSDEIRILAGAYSRALSGTPQAQNQEKAAYNLAQGYFNQALGLWYAGEKFSP
                     EAKADVEAKVAKMIEVYKSRLETADWLAQETRDKAIVKLNVIKPYIGYPEALPERYYK
                     KIVDPSKSLVENAIELNKIDIAHGWSKWNKPVDIKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDSNGASFDEHGSLNNWWTEEDYAA
                     FEARTQQVIDQFEGQDSYGAKINGKLTVSENIADLGGIAAALEAAKSEDDFSAEEFFT
                     NFARIWRMKARPEFMQMLASVDVHAPGHLRTNIQLPNFDEFHETFGVQEGDGMWRAKE
                     DRVIIW"
     gene            complement(14334..15173)
                     /locus_tag="AC1PNG_000171"
     CDS             complement(14334..15173)
                     /locus_tag="AC1PNG_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIP6700714.1"
                     /translation="MFEVLLILMVIASSCGLLGSILVVKNQSMLADALSHSVLLGIVL
                     GFFISHSLDSPLLIVGASLFGLLSVLAIDRLHSRKIAHDAATGLVFSFFFAVAVLLIS
                     LFARNVHLDVDLVLQGEVLFAPLHRMDVLAWSLPVSLVKSSLAWLVIVLFFAWAYHRL
                     QVYLFDSNHARLSGLRTRILEMVILILVSLTTVLAFEAIGSMTVIVFLVAPSMAALRW
                     VKSFWQLLLLGQGIAILTVVLGFLVANQLDLTMSGTCAVVSLLVVCSSIILKNTWSRS
                     SDK"
     gene            complement(15166..16026)
                     /locus_tag="AC1PNG_000172"
     CDS             complement(15166..16026)
                     /locus_tag="AC1PNG_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIP6700715.1"
                     /translation="MFEVFKEYSFWTVALGTVSLAVAASTIGSISVLTKQSLLGDALG
                     HASYPGVIVSFMIFQSRHPLYLLLGAVLSGYLSYVLVHWLRRKGGHSLVNALSLVSAS
                     FFGLGMVLKNAIQGNEAFAGSSQAGLQTYLFGQAAFIQLDDVILIGIISLLALALFTF
                     FYQDYKLYLFDQTFARVIGVRVKYLQQLTMFLMICLIAVGLKLVGAILMSSFLIAPAV
                     FGLMLGKSYHKSLLLAGIVAIGSAFVGTWISSSVSGLSTGPTIIVCLTGLTLSAFVYV
                     TYVRKENGRV"
     gene            complement(16019..16789)
                     /locus_tag="AC1PNG_000173"
     CDS             complement(16019..16789)
                     /locus_tag="AC1PNG_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIP6700716.1"
                     /translation="MSAIIELKDVNLAYTASQTMALEDVNLVIPRGSRTAIVGPNGAG
                     KSSLFKVILGLEKPDTGQVRLLGQEAGLERLIAQKVAYIPQSSQVNWQFPATVFEIVL
                     MGRFAHSKGMFRRPTKADRQIVEQALERLKIADLRHRQIDQLSGGQRQRVFLARALAQ
                     EAELYLMDEPLAGIDQATEIMIMDMLKEFQCEGKTSIVIHHDLTTLDAYFDHLVWLHK
                     HVIDSGPMDETLTSENYQATYGIGNGLFLGNAKGGSHV"
     gene            16833..17102
                     /locus_tag="AC1PNG_000174"
     CDS             16833..17102
                     /locus_tag="AC1PNG_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700717.1"
                     /translation="MKIKTRPTYQAEKVDKIKIPYQQIAGKPYTSLISTHQYMNLIFA
                     EHQCFLQSYVYKSVKNGPKTERTRPRPSPFYYSFEIPIHAQTTDI"
     gene            complement(17343..18266)
                     /locus_tag="AC1PNG_000175"
     CDS             complement(17343..18266)
                     /locus_tag="AC1PNG_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter solute-binding protein,
                     Zn/Mn family"
                     /protein_id="HIP6700718.1"
                     /translation="MKKILFSFALLLSLIGLGACRPSQTTEGSSKPRVAVTTSFLNDM
                     VYQLAGDEVERDLLIPAGEDPHLYVAKSSDLSKLQKADLVLYHGLHFEGKMVEALEKT
                     GVAVSKNFNAKDLNTMDEDGEEIVDPHFWFSIPLYKSAVAVASEELQKLLPAKAEMIQ
                     KNAEKYQAQLDDLHAWVEKELSVIPKESRYLVTPHDAFNYFAASYDFTLYAPQGVSTD
                     SEVANSDMIETVNLIIDHNIKAIFTESTTNPERMKKLQEAVKAKGGQVEVVTGEGKEL
                     FSDSLAPEGEEGDTFIDMYKHNVKLMVKYLK"
     gene            18392..19051
                     /locus_tag="AC1PNG_000176"
     CDS             18392..19051
                     /locus_tag="AC1PNG_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIP6700719.1"
                     /translation="MTPNKEDYLKCIYELGQLDQKITNKLIAEKMAFSAPAVSEMLKK
                     MVAEELISKDAKAGYLLSQTALEMVASLYRKHRLIEVFLVEQLGYSPEEVHEEAEILE
                     HTVSDHFINRLDLLLEQPQTCPHGGSIPQAGQPLIERYQTRLSQLTETGNYQLVRIHD
                     FYQLLQYLEQHELAVGDLLTVTAFDQFAQTITIQYKDKELAVPTAIAQQLFIEKSNRP
                     A"
     gene            complement(19098..19361)
                     /locus_tag="AC1PNG_000177"
     CDS             complement(19098..19361)
                     /locus_tag="AC1PNG_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700720.1"
                     /translation="MRKFFQDLNQEYLETRSSVGEDWLEWIIQRKMTWGMRWGLVGIL
                     MFVLQPLTYNPHFLLGCFYIGFLLWLVYVCGQLFLQPDLFSVI"
     gene            complement(19467..19652)
                     /locus_tag="AC1PNG_000178"
     CDS             complement(19467..19652)
                     /locus_tag="AC1PNG_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700721.1"
                     /translation="MKKLLTNHFFYLTIAFLLILVIYFSGIDKRWIILASFLYFIPSQ
                     ILYRRRLRERLQEDQPE"
     gene            complement(19728..20171)
                     /gene="dtd"
                     /locus_tag="AC1PNG_000179"
     CDS             complement(19728..20171)
                     /gene="dtd"
                     /locus_tag="AC1PNG_000179"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIP6700722.1"
                     /translation="MKIVLQRVSQASVTIEGSIHGQIEQGLLLLVGVGPDDTQEDLDY
                     AVRKIVNMRIFSDEAGKMNKSVQDVAGKILSISQFTLFSDTQKGNRPAFTGAAAPALA
                     SQLYDAFNQALSAFVPVEVGVFGADMAVSLVNDGPVTIVLDTKNK"
     gene            complement(20173..22374)
                     /locus_tag="AC1PNG_000180"
     CDS             complement(20173..22374)
                     /locus_tag="AC1PNG_000180"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIP6700723.1"
                     /translation="MKEINFTGEEVVGLTATYLPAEDVAFVQKALDFATEAHKLQFRK
                     SGEPYIVHSIQVAGILAGLKLDAVTVACGFLHDVVEDTDVTLDDMEAEFGPEVRHIVG
                     GVTKLGKVEYKSHEEQLAENHRNMLIAMSQDMRVILVKLADRLHNMRTLKHLRKDKQE
                     RISRETMEIYAPLAHRLGISSIKWELEDMSFRYLNEVEFYKITRMMNEKRREREELVN
                     EVVDKLRDYTEERHLHGQIYGRPKHIYSIYRKMHDKKKRFDQLYDLIAIRCIMDTPSD
                     VYAMLGYIHELWKPMPGRFKDYIANQKANGYQSIHTTVYGPRGPIEFQIRTMEMHQVA
                     EYGVAAHWAYKRGGKATASEKELRWINNLIELQEGAGDAQTFVNSVKDDIFTERIYVF
                     TPDGAVRELPKDSVPIDFAYEIHTKVGERATGAKVNGRMVPLTTKLKTGDQVEIITSA
                     NSFGPSRDWVNLVKTHKARNKIKQFFKNQDKELSVSKGREMLQNLLQENGYVPNQYLD
                     RRHMDEVLQKTSYKTDESLFAAVGFGEVSAISIFNRLTEKERREAERAKAKAVADELV
                     NGGEVKHDNKDSLKIRHEGGVVIEGASGLLIRIAKCCNPVPGDEIVGYITKGRGVAVH
                     RVDCMNLKSQENYDVRLIDVDWEDENSTKEYMANIDIYGLNRSGLLNDVLKVLTNASK
                     NISSVNAQPTKDMKFATIHVSFGISNLATLTSLVDKIKSVPEVYSVKRTNG"
     gene            22638..25127
                     /locus_tag="AC1PNG_000181"
     CDS             22638..25127
                     /locus_tag="AC1PNG_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HIP6700724.1"
                     /translation="MKFRFSKCALTLSLALLASTNNALVSAEETSTDTPVLKTETEIS
                     SSVATSQAEATTGEVATPTTTETTAGEAVTPTTETTDQSVTPVTEEAAPVVETSNTPV
                     NDTTASPVTATRAAATETTTPVEGQSVDVRILATTDLHTNLVNYDYYQDKPVETLGLA
                     KTAVLIEEAKKENPNVVLVDNGDTIQGTPLGNYKSIVDPIEEGEQHPMYAALETLGFD
                     VGTLGNHEFNYGLAYLEKVIRTANMPLVNANVLDPTTKDFLYTPYTIVKKTFTDTEGK
                     KVTLNVGVTGIVPPQILNWDKAYLEGKVIVRDAVEAVRDIIPTMRENGADIVLVLSHS
                     GIGDDQYEVGEENVGYQIASLSGVDAVITGHSHAEFPGTAEKPSFYAKYSGVDDTNGK
                     INGTPVTMAGKYGDHLGVIDLNLVFKDGKWTTTSSKAAIRKIDTKSSVADGRIIDLAK
                     EAHNETINYVRQQVGETTAPINSFFALVQDDPSVQIVNNAQIWYAKQQLAGTSEANLP
                     ILSAAAPFKAGTRGDASAYTDIPAGPIAIKNVADLYLYDNVVAILKVNGAQLKEWLEM
                     SAGQFNQVDPSSTEPQNLVNTDFRTYNFDVIDGVTYQYDITQPNKYDRNGKVVNETAS
                     RVRNLQYNGQDVTADQEFIVVTNNYRANGTFPGVREASVNYLLNLENRQAIINYIIAE
                     KVINPTADNNWTFTDSIKGLDLRFLTADRAKELVANQEGLVYLQASAENEGFGEFKFV
                     YTEPKVATPDEQQSDQGNTGQDIVLESGQRITLPAVNPPAPAPQHKLASPHSQASTKT
                     LPATGEATSMLSLLGLTLIGFVGAWTKKKEH"
     gene            complement(25248..25805)
                     /locus_tag="AC1PNG_000182"
     CDS             complement(25248..25805)
                     /locus_tag="AC1PNG_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700725.1"
                     /translation="MKRLSTPIFAALGFFLIVFVALNQFGRVPGTSYQIISELVLYNQ
                     ATEQTYEFENHEVRKAFIRQLSSTSSRGFQASDSRTSLETTYHETIMSENLISSIYGG
                     EEGRIMEFSRPRKSTLGNLVFASESVSYLVFARKYGNIRLETTILCQEFRVEQRLANQ
                     SNWVDLGTYTVKSPKETRYVPIYWE"
     gene            complement(26046..26761)
                     /locus_tag="AC1PNG_000183"
                     /pseudo
     CDS             complement(26046..26761)
                     /locus_tag="AC1PNG_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636729.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
     gene            complement(26771..28033)
                     /locus_tag="AC1PNG_000184"
     CDS             complement(26771..28033)
                     /locus_tag="AC1PNG_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700726.1"
                     /translation="MSELEQKEHRELIVSDDCFALIQKMSELDSSAYQRYEITNPNIL
                     AHVEKIVQPALKHHLKKAGQPNIPKGLFKVDLPIGDLHKIKGETDKYRAFVKGAEGKF
                     KAHAILTPAQVEIAKGAKAAAAVSGAMQVAAMVVGQYYMAEINGQLDSMKETLNDVKD
                     FQVREFKGTVRALIVNISQLSKFSADYVENAELARLKLMECANFRAEVTKLLEQVNLA
                     IEESITKGKGLSFEKYEQLIDKLSTNLFYQQTLLRLLAELSKLDLVFSQGVASEDSSY
                     YVYEYYLNNCNSLGEKIANWHKDKIKFFQIDTKRNRRTQEGPMKVVADIAKELDKNAH
                     VAAGVGFLGGLVGAAAGFGIGKAAGELNKAIRYIDVRKRTVKLISEQETVSYQSSKRL
                     SVNNYGENVKLLVEDGKVYYLLPDDSES"
     gene            complement(28047..28793)
                     /locus_tag="AC1PNG_000185"
     CDS             complement(28047..28793)
                     /locus_tag="AC1PNG_000185"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIP6700727.1"
                     /translation="MQQYFINGRAPQGVFQISDKDTAKHMFSVMRLQADEQIVLVFDD
                     GIKRLARVVDSQNQSVEIIEELSDNVELPVSVTIAMGFPKGDKLEFVAQKATELGMAA
                     LWAFPADWSVVKWDGKKLGKKAEKLEKIAQGAAEQSKRNRIPAVRLFEKKVDFLAQLA
                     GFDQIILAYEEAAKEGEQANLVKILSGLEIGQSVLVIVGPEGGVSPEEVAAFEGAGAV
                     KTGLGPRILRAETAPLYALSAISYATELLR"
     gene            complement(28795..29748)
                     /gene="prmA"
                     /locus_tag="AC1PNG_000186"
     CDS             complement(28795..29748)
                     /gene="prmA"
                     /locus_tag="AC1PNG_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIP6700728.1"
                     /translation="MNSWQELTIHVHRDAEEAVSNLMIETGSQGVAISDSADYVGQED
                     RFGELYPEVEQSDMIVITAYYPDTVDIEEVKADLATRLADLTGFGLETGQVSLESQEL
                     AEEDWADNWKKYYEPARISHDLTIVPSWTDYEATAGEKIIRLDPGMAFGTGTHPTTKM
                     SLFALSQVLRGGETVIDVGTGSGVLSIASSLLGAKEIYAYDLDEVAVRVAQENIDLNA
                     NTSNIHVAAGDLLRGVDIKAEVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIADK
                     WDMVRASAEAAGFFLETHMIQGEWNCCVFKKTADRSGVIGG"
     gene            complement(30344..30697)
                     /locus_tag="AC1PNG_000187"
     CDS             complement(30344..30697)
                     /locus_tag="AC1PNG_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HIP6700729.1"
                     /translation="MVHEMLLAPKPFEMMKSGQKTIELRLYDEKRKHIQIGDRIRFYC
                     TENQTQTIEVQVLDLHIFDNFAQLYKELDLLSCGYTQSSIRGAKPEDMEDYYSREQLE
                     QYGAVGIELRVIDSI"
     gene            complement(30699..31169)
                     /locus_tag="AC1PNG_000188"
     CDS             complement(30699..31169)
                     /locus_tag="AC1PNG_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HIP6700730.1"
                     /translation="MAKHGFLDVLEAALDKHFTYDYELNWDKKNHAVELAFILEAQNV
                     AGIETVDDEGNASSEDIFLEEYVLFYNQDKSRFDEEDYLVALPFDAKKGFSAEFLTYF
                     AGFLKDTADQGLDDLMDFLVDPEAQDFAISWDGEAFEKGRADLLEGEFYPYPRY"
     gene            31243..>32525
                     /locus_tag="AC1PNG_000189"
     CDS             31243..>32525
                     /locus_tag="AC1PNG_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIP6700731.1"
                     /translation="MPANLALRMRPKSIDEVIGQEHLVGPGKIIRRMIDANMLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATTDNQKRLQEIAEEAKFSGGLVLMLDEIHR
                     LNKTKQDFLLPLLENGNIIMIGATTENPFFSILPAIRSRVQIFELQPLKTSHIRQALE
                     LALTDSERGFDFPVTIEPEALDFLANATNGDLRAAYNSLELAVLSTKESDDGSRHIDL
                     DAVENSLQKSYISMDKNGDARYDILSALQKSIRGSDVNASLHYAARLIEAEDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALEAAQKIGFPEARILIANVVIDLALSPKSNSAYL
                     AMDAALADLRKNGHLPIPNHLRDGHYAGSKELGNAIGYQYPHAYPEKWVDQQYLPDKL
                     LAADYFTANDTGKYERALGMTQEKIKNLKKNRRQNP"
BASE COUNT         9380 a         7839 c         6192 g         9114 t
ORIGIN      
        1 agccaatata ttttctatca gagacattaa aaattttaat caagtatcgg agcataaaaa
       61 aagacctact cggtctttat atctcatatt aattatactc cagctggtgg gtaaatatag
      121 ctgactgttc cttcgctagt cgttgttgga tcaaagaaac ctcggacatt acttgggtag
      181 tatactccac catagtttga ttccttaact tgaatgtgac cgttagctgc cacatctgtt
      241 acataggcaa catgaccgta gcctccatct gtccatacag caatagcgcc aacttttgga
      301 gtagttccca cttcatatcc aagcgctgcc gcactagctg cccaatctcc accattaccc
      361 caatagtttc caacccaagg agccatttct ttggcacccc aagtacactg accagctgga
      421 taggtattag ccgtatattc cacagcaacc tctgacacca tattggtagc caaagcagcc
      481 tttgcagcgg cagcagcttt ctcagccaca attttttctg cagcagcttt ggctgctatt
      541 tttgcagctt caaccttttt tgttaacgct gatactgtcg gactatcgct tataactgtg
      601 tcatctgcac tgacattcac cattcccaac cataaaagat ttcctatgag ggctgtaaac
      661 agtcccaatt tgaattgttt ttttatcatt tgataactca taatatttga ttccttcgtg
      721 ttttttctgc cctatctatc ctaagggata atccttgcag agagcttaaa gaaatgtaac
      781 aaatattaca gataacttgc aatctcttaa caatcacagt gttcggactt tttgtcaaaa
      841 aaacgacctc caatacggaa gtcgttttct atttagtgtt agtgtaatta ttttctccta
      901 ctcctgagta cactgattag aataaaaacc aaaccagata taatgtttag atatttattc
      961 ccataaagcg gactaatcgc gataaggaaa aatcctagcg ccaataagaa aggagatttg
     1021 ttcttggaaa acattattcc tcaccagcct taaccttatt ggctgcaata acaaatggtg
     1081 tccaaatcag gaaggcaaca aagaggttga atagtgaaac caacgcagcc ataatgttac
     1141 cacctgttgc tagataagcg tagagtcctg gaggggtaat ccatggaaca ggagcaaaga
     1201 ctggcggaat tatacctgcc gctgtcgcta agtatgcgat agttgcacaa atcggtggaa
     1261 caattagcca tggaatagca aacagtggat tcaataccat tggtatacca aaggtaactg
     1321 gttcgttaat attgaataca cccattggag cagataattt agctaccatt ttgtattctt
     1381 ctcgttttga gaaaatgaaa atagcaataa tcaaagctag agtgacgcct gaaccaccca
     1441 tttgagcata agcatcaaag gaaccacgag tccaaagcca cggtaaattc gcagtactat
     1501 gagttgcagt ataagcagcc gcattttcat ttagtgcaac catatagact ccgtccataa
     1561 ttggcgccaa gacattgtga ccatgcaggc cgaagaacca aaaaagttgt acaaggaaag
     1621 ttaaaagaat gactgaccca agaccttgtg acagaccaag taatggcagt tgaatataag
     1681 ttgatactaa atctcccaaa gattgtcctg ttagagtaaa tgcaagatat gccacaatgg
     1741 atgacgcata aattgcagca gtacctggga taatagcagc aaaggcctta ttaactgccg
     1801 gtggaacaga atctggcaat ttaataatga cattccgttt tacaagactt gcatagaccc
     1861 atgtagcgag aaatccaata aacaatgctg taaacaaacc tgttgcaccg ccatatttca
     1921 atgcgatagc tccccactgg cttgtttcca aggctgaaac tccatctgtc gtcacaatgg
     1981 ataatcccaa accttccaaa gcagaaacaa ctgacttatc aactccgtct aaagtagtgg
     2041 aaatcgttac actttgagga atagtagaga tgaatgatgc gaaggaaatc aaaccacctg
     2101 ctaaaggatt caccttatac atgagtgata aattgtaacc aaaggtaaag gcaaatacaa
     2161 gtgccataat cccgatggtt ccaaaataaa cgtagccatt tatatctatc actggttgca
     2221 tggcatcagc aaaaccagtc catcccatat tatttgggat gtctctcaga aagacgttca
     2281 atagaacggc tactgtacct gccatcgtaa tgggcatcat agaaataaag gaatcacgaa
     2341 tcgcaatcaa atgccgttgc gaaccaattt tcccagatat ttccatgaat ttctcagaaa
     2401 tatttgacat attctgtctc cttttctatt ttttgtttta tatgtcctaa atgttaaact
     2461 gataccggct caactgagca acgaatcagc gcctctaact gggcatttgt ctgacgtttt
     2521 atactttttg aaaagatttt ttccatgaga aattgattgc cttttacaaa aatatcacca
     2581 gcctccatct cataagaata ttctatattg gtttgatcat tccctaaatc ctctaactga
     2641 tagcgaacat gctcttttcc tcgattagaa tggaaacaaa ctgcatagca ataaggagct
     2701 gtaaactctt ctacagttac cttgacacta tgttgtccct tatcaccaaa tgttttagta
     2761 taagtaagtc cctggataat gtcttgatct tgcaagatgg aacccgtatt ttgctgaaaa
     2821 tcatctttta aggattggcg aatagtagcg aaaagtcgct caattggaac ttgacctatt
     2881 tttgatatct tcataaccca gttctcctat aattatccaa tctgtttata aagatctacg
     2941 aactctttcg ctaaatcaat aaatgtcaaa gcattcatca agtgatcttg tccatgtacc
     3001 attaacaaag ataattcaac agactctcca cttgcttctt ttgctaataa ttgtgtttgc
     3061 gatttatggg caatattcaa tgaagctatt gcgtcttgga gcagctgttc cgcttcgtca
     3121 atacgacctt ctttggctgc ttgaatcgcc tggctagcga agccctttgc ttcaccaccg
     3181 tacatgatta accccataat cgcttcaaga tactctggag aattcatact acccctccat
     3241 catttttagg gcagcctcta gcaccttagc tccgttcatc actccgtaat ccatcatatt
     3301 gatagcatct accttaatag ttggctcaaa ttttgcctta tagtcgttca ataggaattt
     3361 tacttgtgga cctaagagta atacatcaat ctctttttga gcaacttcat tatcggcttc
     3421 agatactgga acagcccaaa tagttgcttc aacccctgtt tcttctgatg ctttttgcat
     3481 cttagttaca agcatacttg tagacatgcc tgcgttacaa actaacataa tgtgtttcat
     3541 aaaaaactcc tcctacccct ttctttgaat gaggggaata aaaatatcaa ttaattcttt
     3601 ataatttgga tgatgtaaca aacgttcttg gagaactcta tcttgaacaa aatcaaccag
     3661 acaaggtgag atatacttca gaaatgcatt tcgacccttt gacggtgaca ataggaagac
     3721 gaattgaact tgctttccat ggccccaatc cacagcattc ttatatatcc ctacaacaat
     3781 ttgttctgaa taactcatcg gcaaggctgg atgagggaag gctaaccaat ccccaaagac
     3841 aaccggactg taagattctc gtaaattcac ttgttctata aagcgattaa caaaatcagg
     3901 ctcaatggat tcgttcaaag tagttactag ttgcttcaag gctacttctc gatcacaatc
     3961 ctcctcaaga tagattattc tatgtggagc aaatactgct tctgcaattt gcttagcctg
     4021 acctacttcc attagcacac tttccccttg gtcaggaata actggggtcg tttgctgtaa
     4081 atgagatcga atagcctcaa tgtccttctc tgataggaag acgctgacat tgataaccgg
     4141 tgtcataaaa attagattcg acaagctaat ggatgaaata atcaaatcaa catcttttag
     4201 tcgttcctct gtaacttcat aataactaac cgtatcaaga atttctaagc tggatgaaaa
     4261 ttctttctcc aagcgattct ttaacatcaa tgcacttcca attccggtag cacagactac
     4321 caatactctg gtcttcttaa atccctgata acgttcaatt gccgccatca catgcaaggt
     4381 aaggtaagcc cactcatcat ccgaaatcga ataccccatt agttctggta tcgctgcaaa
     4441 tgccgttttt gttaattcga aagcctcaaa atattggtct ttaatttcag cagttagggg
     4501 atttcccagt tgaatattat tctccaaacg aatacgtaac ggcatcaaat gggccagaac
     4561 tcctttgaat agctgctgat cctttgacaa ctctaggcct gttaaatgtg acaaatgtaa
     4621 aagactattc tccacattct gtctaaatat ctcttccacc tcaccatctt gatttggatt
     4681 ccccgttagt tttaccttaa ggtgcaacgc aatgtaattt gcttcctctg acggtatcgt
     4741 cacttgaaac atttcttcga ttcgttcaat aatccgttgt gccacaagat actcttgaga
     4801 cgactgaata cgctccggta ttgggaaccg gtctagacca gatccaattt tcaaccgttg
     4861 taaaagaaga gcgatatgga gcactagatt ctgcatgaca aaatctgata aacgtaattt
     4921 agcatcccga cactcgtcta agacgattaa cattaagtct gtgaaccgta tatctggcaa
     4981 tagattggtt tcgacaaccc cataaacaga ttgaacattt ccctcatcaa aaaagtaatc
     5041 cataataaag tgacggatag caggttcatc cccctttagt tcaattcctt tacgtgtcac
     5101 aaggagttct aattgatagg ggataaatag ttttcgaata tccgccaaaa tggaatgaag
     5161 cgtagtcttt ccaatgaata actctctttg taaacattca ggagactgaa ctggatcctc
     5221 gaacaagagt ttgttcagaa tataccgttg ccgatcaccc gcttcttcta gttgagttaa
     5281 atcagttaac cgctttttta ggagcaattg ctcctgccaa aacatttcaa actcaactgg
     5341 ccgttcaatc tgtaaacaat aaccactacc ttgttttgaa aggatatgag cgccgctatt
     5401 ttctaaagaa gatgtcagtt gatgaatata cgttcttacc gttcgatcac tcactgataa
     5461 taccttggct agctcgatgc ttggtacgaa tgtctcctgt tttgcaagta aagcctctaa
     5521 caagcgtact tcttttttag ataacatgct ccctcctttc cgccaacagt ctttgatttc
     5581 ttatatgtac attataacat gaaagcgatt tctagaggtg cctacttttt ccgcatcatt
     5641 aatgaaagtg ctcggaagct atgaccagtt taaactcttg ccacggttta ataaagcgta
     5701 acaagggaag atagtctggg ctgattcgtc cgaccttatt tacctttgga ctgatggtaa
     5761 attctttgag tgcaatttgc aattcaccct tgtagcgacc atataaatca ttatcaatta
     5821 agatatcccc tcgtcccaca ggaattgctt ggtctgaccg agccggtaca gatgcgtcct
     5881 tatacaccac acggggcatg gttgaacgca gaacataagc agaaatatca cctctataaa
     5941 catgatcaaa cgctacaatc tctctttcaa cgggagtcaa accctcctca atatcaacca
     6001 taaggctgat tgtatcttca tttaaaactg tttgaatgga ttgtagttcc tcctccgaga
     6061 taaattggtt tgaaatcaaa atagaatcaa taaaacccgt tgccttcatc caagcaattt
     6121 gcaagggaag aagccagaat cgatgatctt ctaaagttgg tagaccttca gataatggcc
     6181 acggcccctc agaagctgtt tgtgcactga caaatgccgc agttcttata ccggattgat
     6241 aataaaaaga tgacatctct aagaagtgct cttgggataa acctgtataa gcatgaggat
     6301 aaaaattatg gcaggcaata atattatccc tgtttgcaac actagccaaa agattggaca
     6361 gcaaggtcct atctgtactc aaattcaatt caacttttat gttgtgtgga ttatgtgtca
     6421 tctcaatgat ttcttccaaa ggaagagctt catcaagacg aatcccagcc aaccccagtt
     6481 cagctgcacg ctccatcaat gccccatccc aaccttgcgc tgcgataaaa tggggactga
     6541 tatctgcaat gacagtcatc cctagttgat ttgcatagtc aactagtttt tgatacaatt
     6601 caaaaactgt tcggtctcca ttttcaagtt gcaacaaact cataaataag cgccttgctc
     6661 cgtattggtg cagaagttta atgtaagctt gactctcttc tagcgaatag ttttcaggat
     6721 aaattgaaaa gcccagttct accatcattc cctcctagcg atgaaaacca ttagcatcgc
     6781 tcaatttctt gaaccacttg cctgatttct taacaattct ttcttgcgtg tctagatcca
     6841 gttcaactaa tccatagcga ttcttgtatg catttaacca agaccagcaa tcaataaagg
     6901 tccataacat gtatccttta caattcgccc cttcttggat agctcgatgt aattcaatta
     6961 aatgatcttc gataaattca atccggtaat catcttgaat gagtccattg tctttaaatg
     7021 cgccctctcc ttcaactccc ataccatttt ctgttacaaa ccattcgata ttatggtaat
     7081 tttccttaat atttatcgca atatcatata tccctttttc ataaatctcc caacctctat
     7141 gcggattcat ttttttacct ggcatatcat atggggcaaa gaaatgttcc ggagtaagag
     7201 gctctccctc tttccaacta ttggttggag cttgtacccg aaggggttga taataattca
     7261 cccctaggaa gtccacacga ttttccttaa taatggctaa ttcctcatcg ctgacctctg
     7321 gcattagccc atatgcatca atcagctcga ctaattcatc gggatattct cctaacacag
     7381 acggatctag aaaactcttt gtttgaaaca attctgcaat tcgagctgct tttatatcct
     7441 ctgcttgctc actacgaggg taggcaggag ttagattcaa gacaatagcg atttttttgt
     7501 gaggatttac ctcatggcat gcctttacag ctaaagaact tgccaactgt gtatgataag
     7561 ccaccgcaac tgccgctttt gcatcaacct tacatggata atgataattt cctagataac
     7621 cacactctac tggaacaatc ggctcattaa aggtaatcca acgatcgact aagtcatcaa
     7681 acaattcaaa acatgtacgt gcatatgctt cataggctcg aacaatttcc ttattttccc
     7741 aaccaccttt ttcttgtaaa caatagggaa gatcaaagtg ataaagattg acaaagagtt
     7801 gaattccctt tgctcttacc aaagaaaaaa ccgaacgata gaaggacact gcctcttgat
     7861 taatttctcc agtacctgtt ggtataagac gtgaccactg aatggaggtc cgaaatacag
     7921 tgtgcccagt ttcaactaat aaatcaatat ctttttcata atgtttgtaa aatgttgatg
     7981 tttgactagg gccaattttg ttatgaaatc gttcaggctc aatactatac caatagtccc
     8041 agttgttctg tccttttcca tcacctggct caattccctc actttgaggt cctgatgtag
     8101 aagaacccca caaaaaattt gttggaaatt gataaaatac cttgtcttta gtcattgatt
     8161 gttccttctc tcttttatgt gctgtatagc tggtgcgaat tgtggcaaat aattctcatg
     8221 tgcaagtaac atatcttgta atagcacttc agcttgtggt ccgtttgaaa taagcgggtt
     8281 gagtgtaaaa gcttgtaaag ccagtgaata atcacctgtc attgctgcct gaacagtgac
     8341 aagctccatt tgcttcatca gttgtaaata gccggttaca atcggcggag ttggtggaca
     8401 agctagtggt gttgctcctt gcgcagagat aatcgaggtg acttctacca cacaattttc
     8461 aggtagatag ggaataactc ctctatttac agtagataca gtcaattctt ctctactatc
     8521 attatggata gcacaaatga tttgacaggc aacatcacta tagtaggcac ccccacgtag
     8581 ttccagttgt tttggtttat ctttcaattg tgggtctttg taaagctcaa acaattctgt
     8641 ctctacttct tgaacaacct tggctcgtac atttccctga cgatactgct ccaaagcatc
     8701 ctctaacatt tcttgttcca aaaaataata acgatggtaa tcacatggca acattccaat
     8761 cgtccgaagc aattgaatag gaaactctag attggtaata tttttgactg cttctggcgt
     8821 ctcctctctc aaatccccat acaatgtttc taagattact tgcgtacgat tttccccata
     8881 cttatcccaa acttcatgaa agtgaaaatg atttaaccca atatggcgcg tacttaggtc
     8941 tccttctgat tggcctaaaa tctgagcagc aagtttcttt tgaccaatag ggatattaca
     9001 caaaccaata actttcttcc agtggagttc attagcaatg gcttccgtta cgataccagc
     9061 aggattcgta aaattaatca accaagcatt cggacacaag tccttcatat ccttgataat
     9121 ccctttatat gcttcaatgg tcctaaatgc tttaaagatt cctccagccc cattggtctc
     9181 ttgccccagc atcccataac tcaagggaat ggtctcatct aagtatcttg caggtagttg
     9241 gccaactcgg aactgagacg ttacaaaatc agcctctatc aatgcttctc ttctgtctaa
     9301 ggtcgactct atcttccagt ccaggcctgc ttttgcgacc attcgccgtg ctaactcaac
     9361 tacgatctcc tgtttttttg cgccttgttc aacatcaaca aaccaccatt ctcgaatggg
     9421 taattgatgt gcacgaagaa tcataccttc aaccaattct ggagtgtaac ttgagcctcc
     9481 accaattgtc actaatttaa gagttttcaa cacgctttcc ctccctcatc ttcttttata
     9541 ttcttattat atttcagtga acatctattg taaacggata cacagaccct ctttttccgt
     9601 atcacaacgg aaattaagac aacatgatca ttctagaaaa tacaaaaacg acctccaata
     9661 cggaagtcgt cttcttatta tttacattta tttcacaaca atattaacaa gtttagttgg
     9721 ctccaacagt ctgcggatag actgttggag gttggaaata gacaaacaaa cgaggcaatc
     9781 gttacttggt aacgatattg acaagtttgt ttggaaccgt aatcaccttc accactgtct
     9841 gcccagcaat ttcagcttgg actttttcgt ccgcaagggc aactttttca agctctgctg
     9901 gtgctaggtc tttggctacg gtcaagcggg cttttacctt gcccttgatt tggacaacga
     9961 tttccacttc gctttctacc agcttgcttt cgtcatatgt tggccatgcg acatagctga
    10021 ttgactgacc tgtatttgtc agaccttgcc agagttcttc tgccaagtgt ggcgcaaatg
    10081 gagcaatcaa ttggacaaag cctttggcgt attcgacata gagcttgtct tctttgttgg
    10141 ctgcgttgac aaaaatcatg agctgggcga tagccgtgtt gaatttcagg tcttcaatat
    10201 gctctgtcac cgtcttcaca gtttcattgt aaactttatc aagcgctccg ctattttccg
    10261 ccgcaagctc cttggtcgtc aagaggcgat aaacacggtc aaggaactta cggctgccct
    10321 cgagaccttc ttctgaccag gcgattgacg catcaagtgg gcccatgaac atctcgtaaa
    10381 cacggagggt atccgcaccg aactgctcga ccacatcgtc tggattgacc acgtttttaa
    10441 gggacttgga catcttagca ggtgcctgct ccaactcctc accagtttcg atgttgaaga
    10501 aagaaccatc acgtttttca actttatctg tcgcaacaag ggcaccacgg ctgttacggt
    10561 agctagttcc caaaatcata ccctggttaa atagtttttg gaaaggctct ttagtcggca
    10621 caacgccaag gtcatagagg aacttgtgcc agaagcgagc ataaagcaag tgaaggactg
    10681 cgtgctcggc accaccgatg tagatgtcca ctggcaacca agctttgaga agatcctcgt
    10741 ctgccaactt ctcatcattg tgcgggtcga tatagcggag gtagtaccag ctagaacccg
    10801 cccattgtgg catagtgttg gtttcacggc gacctttaac gccgtcttcc cgcaccactt
    10861 ccaaccagtc tgtcaagtta gcaagaggtg actcccctgt acctgaaggc ttgatgtctg
    10921 aggtttttgg caataccaga ggtaactcat tttctggtac ggctgtggaa gtgccgtctt
    10981 cccagtggat gattggaatt ggctcacccc agtaacgctg gcggctgaag agccagtcgc
    11041 ggaggcggta agaaattttc tcttgaccaa caccattttc ttccaaccaa gctaccattt
    11101 tggcaatagc ttcttccttg ttaagaccat ctaggaaatc agaattgatg tgggcaccgt
    11161 cctctgtgta aggagcttct tctacattgc caccttccag aactggaatg atgtccaaac
    11221 caaactgttt ggcaaattcc cagtcacgct catcgtgggc aggaacggcc atgatagcac
    11281 ctgttccgta gcttgccagt acatagtccg caatccagat tggaatttct ttaccgttga
    11341 cagggttgat ggcgtaggca ccagtccaaa cacctgtctt gtcttttgcc aaatctgtac
    11401 gggctaggtc tgatttgaga gaagcttggc gtttgtactc tgccacagct tctgtctgct
    11461 caggcgatgt aatgctatct accaagtcat gctcaggagc aagaacagca taagtcgcac
    11521 caaaaagggt gtcaggacga gttgtaaaga ctgtgaagtc cttgtctgtg tccttaatct
    11581 tgaaagtcac attggcaccc gttgacttgc cgatccaatt gcgttgcata tccttgatag
    11641 actctggcca atcaacttct tctaggtcgt tgagcaaacg ctctgcatag gcagtgattt
    11701 tcaacatcca ttggcgcatt ggcttgcgga caactgggta gccaccacgt tcagacgttc
    11761 cgtcaggaag gacctcttcg ttggcgatag ctgttcccaa ttcctctacc cagttaacag
    11821 gcacttctgc ctcataggct aggccttttt catacaactt ggtgaaaatc cactgggtcc
    11881 acttgtagta gttagggtca gtcgtattga cctcgcggtc ccagtcataa gaaaagccaa
    11941 gggcgttaat ctgacgcttg aagttagcaa tattttccgc cgtaaagtca gctgggtcat
    12001 tgcctgtatc catagcgtat tgctcagctg gcagaccaaa ggcatcccac cccattgggt
    12061 gaagaacgtt gtagccctga gcacgcttgt atcggctgag aatgtccgtc gccgtatagc
    12121 cctcagggtg accaacgtgc aagcccgcac cagacggata agggaacata tctagggcat
    12181 aaaagtttgg cttagccgca tctgttcccg tcttaaaagt atgatttttt gcccaaaatc
    12241 cctgccattt gggctcgatt tccttgtgat tgtagaagct catatcattt ctccatgtca
    12301 ataaattatc tttactatta tatcacgttt tgggggaatt gggaaagaca aaaacagcca
    12361 gttttcacta gctgttttcg catttttacc aaataatcac acggtcttct tttgcacgcc
    12421 acatgccgtc gccttcttga acgccaaatg tttcatggaa ttcatcgaag tttggcaact
    12481 ggatgttggt gcgtaggtga cctggtgcat ggacatcgac actagccaac atctgcatga
    12541 actcaggacg agccttcatt cgccagatgc gagcaaagtt agtgaagaat tcctctgcgg
    12601 agaagtcatc ttctgacttg gctgcttcca aggccgctgc aatcccacca aggtcagcaa
    12661 tgttttctga cacggtcaac ttaccattga ttttggcacc gtaagaatcc tgaccctcaa
    12721 actgatcaat aacctgctgg gtacgggcct caaaggctgc ataatcttcc tctgtccacc
    12781 agttattcaa gctaccatgc tcatcaaagg aagcaccgtt tgagtcaaag gcatgggaaa
    12841 tctcgtgggc aatgacggca ccgataccgc cgtagttggc tgacgaagat tgctccaagg
    12901 agtaaaacgg agcctgcaaa atcgccgctg ggaagacaat caagttcttc tgtgggttat
    12961 agtaggcgtt gaccatgtga gctggcatgc cccactcctt aatgtccaca ggcttgttcc
    13021 acttgctcca accatgggcg atgtcaatct tgtttagttc gatggcattt tccaccaagg
    13081 acttgcttgg gtcaacaatc ttcttataat aacgctctgg caaggcttct ggatagccga
    13141 tataaggctt gataacattg agtttgacaa tggccttgtc acgcgtttcc tgagccaacc
    13201 agtctgccgt ttccaaacga gatttgtaaa cctcaatcat cttggcaact ttggcttcca
    13261 catcggcctt agcttctggt gagaattttt ctcctgcata ccaaagaccg agggcttggt
    13321 taaagtagcc ttgtgccaag ttgtaggctg ctttttcttg gttttgcgcc tgcggtgtac
    13381 cagaaagagc acggctgtag gctcctgcta gaatgcgaat ttcatccgac aggaaggcag
    13441 tataggcgcc agccgctttc aaaatcaagg tcgccttcaa aagctcccaa ttatccgcac
    13501 tgtaaatgtc cttggcagcc tgccagaaac gttcctcagg cacgataatc ttgtctggcg
    13561 tttgacctaa aatcgctgtg aagaaatcat ccaaaggcaa ctctggtgcc aaggcagtaa
    13621 aatctgccca ctcgtacggg tggtaaagtt tggcgtactc agaaccttct tcatttgaca
    13681 gaacatactt ggcaatcgtg gcatctagtt ccaaacgctt gtccaagagg tccttgattt
    13741 cctcatcgct gaaaccaaat tttggcagga gggcttcttg gctctcacgc cagattttga
    13801 gtaattctgc acccttttca tgaccttcct catagtaggt cgtatctggg aggataatgc
    13861 ctagagaatc tgcccagagg acattggtgg tcgcgtccat aaagtcaggt gccacgccga
    13921 aaggcatgag gtttggcttg cctgccaact catattctgc caacttgctg gtgaattctt
    13981 cgaaagagtt gagagccttg tactctgcaa tcaaggcctg agcaggttct gctcccaaac
    14041 ggtcacgggt ttcatagtcg gctacttgtt tgtgaaaagc cacaaagttt tgcaaaacgc
    14101 tatcttctgg gacatcttct cccgccaacc aagcgtttgt cgtgtcaatc atgagctttt
    14161 caatctcatc cgccaagtct gagaaaccac ctgttcttgg cttatcgtct ggaatcacag
    14221 ctgtttttgc ccattcacca ttgacgtatt cgtaaaaatc atcttgtaaa cgtgtcattg
    14281 attttctcct ttgtctttct taatgactat aataacaaaa aattctgaca atttcactta
    14341 tcagatgacc gtgaccaagt attttttaga ataatactcg aacaaaccac aagaaggctg
    14401 acaacagcac aggtccctga catggttaaa tccagttgat tggccactaa aaaacctagc
    14461 acaaccgtta gaatagcgat accttgccct aaaagcagca attgccaaaa ggatttgacc
    14521 caacgtaggg cagccatact tggtgccacc aagaaaacaa taacagtcat cgagccaatc
    14581 gcttcgaagg ccagaacagt agttaaggaa actagaatca aaataaccat ttctaaaatc
    14641 cttgttcgta agccagacaa acgagcatgg ttgctatcaa ataggtacac ttgtaaacga
    14701 tggtaagccc aagcaaaaaa tagaacaata accaaccaag ccaaactcga cttgaccaga
    14761 ctaacaggga gtgaccaggc tagaacatcc atacgatgga gcggagcaaa aagtacttcc
    14821 ccctgcaaaa ccagatccac atccaagtga acattgcgag caaaaagaga aataagcaaa
    14881 accgccaccg caaaaaagaa agaaaaaact agacctgttg cggcatcatg cgctatcttt
    14941 cgactatgca gacggtcaat ggcaagaacc gataataacc caaacagact ggcaccaact
    15001 attagcaatg gcgaatccaa actatgacta ataaaaaatc ccagaacaat cccaagcaag
    15061 acagaatgcg atagtgcatc tgccaacatc gattgatttt ttacaaccag tatcgagccg
    15121 agcaagccac aagaactagc aataaccatc aaaatgagca atacttcaaa cacgaccatt
    15181 ctcctttctg acataggtaa catacacaaa tgcagacagg gtcagacctg tcagacagac
    15241 gataatagtc ggtccggtcg ataaaccaga tacactagag ctaatccagg tccctacgaa
    15301 agccgaccca attgcaacga ttcctgccag taacagactc ttatgatacg attttcccag
    15361 catcaagcca aagacagctg gtgcaatcaa aaaactactc atcaaaatag ctccgactaa
    15421 ctttaatcct actgcaatta ggcaaatcat caaaaacatc gtcaactgct gcaagtactt
    15481 tacacgaaca ccaataaccc ttgcaaaagt ttgatcaaac agatatagct tatagtcttg
    15541 ataaaagaaa gtaaacaatg ccaaagccaa tagagaaata attccgatga gtatcacatc
    15601 atccagctga ataaaggctg cctgtccaaa tagataggtt tgcaatccag cttgggagga
    15661 ccctgcaaag gcttcatttc cctgaatagc atttttcaag accattccca aaccaaagaa
    15721 agacgcagac accaatgaaa gggcattgac taggctgtga ccaccttttc gacgcaacca
    15781 atggacaagt acataagaaa ggtagcctga caacactgct cctaacagca agtagagagg
    15841 atgtcgcgac tgaaaaatca tgaaagagac aatcactcca ggataagaag catgcccaag
    15901 agcatcccca agcaaactct gtttagtaag aacagatata cttccgatag tgctggctgc
    15961 tacagctagg ctgacggtcc ctaaagcaac tgtccagaaa gaatactctt tgaaaacctc
    16021 aaacatgact gcctcctttg gcatttccta gaaacaaccc atttcctatg ccgtaggtcg
    16081 cctgataatt ctcgcttgtc aaagtctcat ccatcggacc agagtcaatc acatgcttat
    16141 gaagccagac aaggtgatca aagtaggcat ccagcgtcgt taagtcatga tgaatgacaa
    16201 tggaagtttt cccttcacat tggaactcct tgagcatatc catgatcatg atttctgttg
    16261 cctgatcaat ccctgctaga ggctcatcca taagatacaa ttctgcttcc tgtgccaggg
    16321 ctctcgctag aaagacccgc tgacgctgcc cacctgaaag ctgatcaatc tgacgatggc
    16381 gtaaatccgc aatttttaac cgttctagag cctgctcaac aatctgtcta tcagccttag
    16441 tcggtcttcg aaacattccc ttactatgag cgaagcgtcc catcagaaca atttcaaaga
    16501 ctgttgcagg aaattgccaa tttacttgac tggattgggg aatataggct actttttgag
    16561 caatcaacct ctctagacct gcctcctgac caagcaagcg aacctgccct gtgtcaggtt
    16621 tttccaaccc caaaatgacc ttgaatagac tggacttccc agcaccattt ggtccaacaa
    16681 tagccgtccg actccctctg ggaatcacca aattgacatc ttctaaagcc atggtctggc
    16741 tggctgtata tgctaaattg acatctttta attcaatgat tgctgacata tttcctccta
    16801 tttttaaaaa tgatatgcct cattcatact caatgaaaat caaaaccaga ccaacctacc
    16861 aagctgagaa agtcgataag attaagatac cgtaccaaca aattgctggc aaaccctata
    16921 catcgcttat ttcaacacat cagtacatga atttgatttt cgcagagcac caatgcttcc
    16981 ttcaatcgta tgtttataaa tctgttaaaa atggtccaaa aacggaaaga actcgaccta
    17041 gaccgagtcc tttttactac tcatttgaaa tccctattca cgcacagact actgatatat
    17101 aaagtcccgc tgtgaaccaa gatcattgag gtctacaagc ggtatttcta gtcaaggacc
    17161 tcaataactg gtgtgatttg ctactaacgc ctatcctccc ccaagcgtta ccacctccaa
    17221 tagaaagagc cctataccgc ttgtgctcga aacaattgat tgtccctcat attggtacct
    17281 atcaactcta tcttcggtaa tatctctagt cttcattgaa tagcaagtcc gttcgatctg
    17341 tactatttca gatatttaac catcagttta acattatgtt tgtacatgtc gataaaggta
    17401 tccccttcct cgccttctgg tgccaaggaa tctgagaaca actccttacc ttcgccagta
    17461 acaacttcaa cctgaccacc tttggctttt actgcctctt gcaatttctt catgcgttct
    17521 ggattggttg tagactctgt aaagatagcc ttgatattat ggtcgatgat taggttaact
    17581 gtctcaatca tgtcactgtt agccacttct gagtcggtgc tcacgccttg aggagcataa
    17641 agagtgaagt cataactagc agcaaagtaa ttaaaggcat catgtggtgt taccaaatag
    17701 cgactttctt ttggaatcac acttaattct ttttctaccc aggcatgcaa atcatctagc
    17761 tgcgcttggt atttttctgc atttttctga atcatctcag ctttagcagg taagagtttt
    17821 tgtagttcct ctgaagctac agctacagct gacttgtaca agggaattga aaaccagaag
    17881 tgcggatcga caatctcttc tccatcctca tccatcgtat tcaagtcttt tgcattaaag
    17941 tttttagaaa cagctactcc ggtcttctcc aaagcttcaa ccattttacc ttcaaagtgc
    18001 aaaccgtgat agagaacaag gtcagccttt tgtaattttg ataaatcact agatttggct
    18061 acgtacaaat gagggtcttc tcctgctgga atcagcaaat cacgttccac ctcatcaccc
    18121 gccaactgat agaccatgtc attgaggaag gaagtagtca cagccactct tggcttacta
    18181 gagccttctg tggtttgcga tggacgacaa gctcctaacc ctattaggga caatagcaga
    18241 gcaaaactga acaatatttt cttcatatag gttctccttt tatttttatt aagcatacct
    18301 aactatataa ttagattaat ctaaaaaaat cattttgtca actgattttt aaattttttg
    18361 atataatagt ttcagaagaa agcgagttct catgacacca aacaaagaag attacctaaa
    18421 atgtatttat gaactaggtc aattagacca aaaaattacc aataaactca tcgcagagaa
    18481 gatggccttc tccgcaccag ccgtttccga aatgcttaaa aaaatggtag ccgaagagct
    18541 catttctaag gatgccaagg caggttatct cctcagccaa actgcccttg aaatggtagc
    18601 cagcctctat cgcaaacacc gcttgattga ggtattctta gttgagcaac tcggctactc
    18661 tccagaagaa gtacatgaag aagctgagat tttagaacac accgtttcag atcactttat
    18721 caatcgccta gacctgctac tggaacagcc tcaaacttgt cctcacgggg gaagcattcc
    18781 tcaagcagga caaccgctca tcgaacgcta ccagacacgg ctgtcacagc taactgagac
    18841 agggaactac cagcttgtcc gtatccatga cttctatcaa ctccttcagt acttggaaca
    18901 acatgaatta gctgtcggtg atttactaac cgtcacagcc ttcgaccagt ttgcccagac
    18961 catcaccatc cagtacaagg acaaagagct cgccgtccca acagccatcg ctcaacaatt
    19021 attcatcgaa aaaagcaatc gcccagccta aacaagctga gcgatttttt cttctaaaat
    19081 aaatagttac ctctttttca aattacggaa aatagatctg gctgcaaaaa tagttgccca
    19141 catacataca ccaaccaaag taggaaacct atataaaaac agcccaatag aaaatgaggg
    19201 ttataggtta aaggctgcaa gacaaacatg agaatcccta ccaaccccca ccgcataccc
    19261 caggtcatct ttctctggat aatccactcc agccaatctt ctccaactga acttctggtt
    19321 tccagatact cttgattaag gtcttgaaaa aattttctca tatttctgcc cacctcatcc
    19381 taacttcaaa gcataaacta ccactttatg acaatattat catagcacat tgttttcaaa
    19441 taaccaatac tgtcgaaaaa accagcctac tcgggctggt cttcttggag tcgctctctt
    19501 aggcgacgac ggtataaaat ctgcgatgga atgaagtaaa gaaaacttgc caaaataatc
    19561 cagcgtttgt caattccact aaagtaaatc actaaaatca aaagaaaggc aatcgtcaaa
    19621 taaaagaaat gatttgtgag caattttttc atagtagcct ccatacctat ttttcaatat
    19681 tagtatatca aaatacacaa atagagtaca taccgttgcc aaaagtttta cttatttttc
    19741 gtatccaaaa caatagtcac cggcccatca ttgaccaggc taaccgccat atccgcccca
    19801 aaaacgccca cttcaacagg cacaaaggcc gacaaggcct ggttaaaagc atcatagagt
    19861 tggctggcta gggcaggagc tgcagctcct gtaaaggcag ggcgattacc tttttgggtg
    19921 tccgaaaaga gggtaaattg ggaaatggat aaaatcttgc ccgccacatc ttgaacggac
    19981 ttgttcatct tgcctgcctc atctgaaaaa atccgcatat tgacaatttt ccgaacagca
    20041 tagtccaggt cttcctgtgt gtcatctggc cccacaccga ctaggagcaa aagcccttgt
    20101 tcaatttgcc catggatact tccttcgatg gtcacactgg cctgtgatac acgttgtagt
    20161 acaattttca tcttatccgt tggtcctttt cactgagtaa acttctggca ccgacttaat
    20221 cttgtccacc agactggtca aggttgccag gttggaaata ccaaaggaaa cgtggatagt
    20281 cgcaaacttc atatccttgg ttggctgggc attgaccgaa gaaatgttct tgctggcatt
    20341 ggtcaaaacc ttgagaacat cattgagcag tcctgagcgg ttgagaccgt aaatgtcaat
    20401 gtttgccatg tattccttgg tcgaattctc atcctcccag tccacatcaa tcaagcgaac
    20461 atcgtagttt tcttgactct ttaggttcat acagtctaca cggtgaacag ctaccccacg
    20521 ccccttggtg atgtagccga caatttcatc gccaggtact ggattacaac atttggcaat
    20581 ccgtatcagc aagcctgacg ctccttcaat gaccacgcca ccttcgtgac ggattttcag
    20641 actatctttg ttgtcgtgtt tgacttcgcc accattgacc aattcatccg cgacagcttt
    20701 ggccttagcc cgctcagctt ctcgacgttc tttctctgtc agacggttaa agattgatat
    20761 ggcagaaact tctccaaaac caactgccgc aaaaagggat tcgtcggtct tatagctggt
    20821 cttttgcagg acctcatcca tgtggcgacg gtccaggtat tggttgggaa cgtagccatt
    20881 ttcttggagt aagttctgaa gcatctcacg cccttttgaa acagacaatt ccttgtcctg
    20941 attcttgaag aactgcttga ttttattgcg agccttgtga gttttaacca agttgaccca
    21001 gtcacgactt ggaccgaaag agttggcaga ggtgatgatt tccacctgat cgcctgtctt
    21061 aagcttggtc gtcaaaggca ccatacggcc gttgaccttg gcacctgttg ctcgctcgcc
    21121 gaccttggta tgaatttcat aggcgaaatc aatcggtaca gaatcctttg gcaactcacg
    21181 cacagcacca tctggcgtga aaacgtaaat acgttctgta aaaatatcgt ccttgacaga
    21241 atttacaaag gtttgcgcat ccccagcccc ttcttggagt tcaatcaggt tgttaatcca
    21301 gcgcaactct ttttcgctgg cagttgcctt gccaccacgc ttgtaagccc agtgagctgc
    21361 caccccgtat tccgccacct gatgcatttc catggtccga atttggaact cgattggacc
    21421 tctcggtccg taaaccgtgg tatgaataga ctggtagccg ttagcttttt ggttggcaat
    21481 gtagtccttg aagcgacctg gcataggttt ccacaattca tgaatatacc ctaacatagc
    21541 atagacatcg cttggagtat ccatgataca acgaatggca atcaagtcat agagttgatc
    21601 aaaccgcttt ttcttatcgt gcattttgcg atagatagag taaatatgct tgggtctacc
    21661 gtaaatctga ccatgtagat ggcgctcttc tgtataatct cttagcttat caacaacttc
    21721 attgaccaac tcttcccgtt cacggcgctt ctcattcatc atgcgcgtaa tcttgtagaa
    21781 ctcaacttca ttgagatagc ggaaagacat gtcttccaat tcccacttga tagaagaaat
    21841 acctagacgg tgggcaagcg gtgcatagat ttccattgtt tcacgtgaaa tacgttcctg
    21901 cttgtccttg cgcaagtgct taagagtccg catgttgtga aggcggtctg ccagtttaac
    21961 cagaatgacc cgcatgtctt gggacatggc tatcagcatg ttgcggtggt tttcagccaa
    22021 ctgctcctca tgagacttat actcaacctt acccagtttg gtcacaccgc cgacaatatg
    22081 gcgaacctct ggaccaaatt cagcctccat atcatccaaa gttacatccg tatcttcaac
    22141 cacatcatgt aagaaaccac aggcaaccgt cacagcatcc agcttgagtc ctgctaaaat
    22201 acctgctacc tggatcgagt gtacaatata gggttcgccc gatttacgaa attgtagctt
    22261 atgagcttcc gtcgcaaaat ccaaggcctt ctgaacaaaa gcaacatctt cggctggaag
    22321 gtaggtggca gtcagcccaa caacctcttc tcctgtaaaa ttgatttcct tcatttgttt
    22381 ctctccctaa ctatttccta ttattttacc attttcactg gaaaagaaaa aggaaagcgg
    22441 taattcatcc atactatacc attcaaaatg acgataaaca aagaaaacca gacaaacaat
    22501 tttttagtca ttgttcgttt tttgttgctt ttatgaaaat ttttctttat ttttcttttg
    22561 tgttttcgta gaaaccgttg atgttttcgt tttcaatatg tataatattc ttgagattat
    22621 atcaaatgga ggttctcatg aaatttcgtt ttagtaagtg tgccctaacc ctcagcctag
    22681 ctttactggc tagcaccaac aacgcccttg tctctgctga agaaaccagc acagataccc
    22741 ctgttttgaa gactgaaaca gaaatcagta gctctgtagc aaccagccaa gccgaagcaa
    22801 cgactggtga ggttgccaca ccgacaacta ccgaaactac agccggtgag gctgtcacgc
    22861 caactacaga aaccactgac cagtctgtta caccagtaac agaagaagct gctcctgttg
    22921 tagaaaccag caatactcca gttaacgaca caacagctag ccctgttaca gcaacacgcg
    22981 cagcggctac tgagactaca actcctgtcg aaggccagtc tgttgatgtt cgtatcctcg
    23041 caacaactga cctccatacg aacttggtca actatgacta ttaccaggat aaacctgtag
    23101 aaacactggg tctagccaaa acagctgttc ttatcgaaga ggcaaaaaaa gaaaatccaa
    23161 atgtcgtatt ggtagataat ggagacacca ttcaaggtac tcctcttgga aattacaagt
    23221 ctatcgttga tcctattgaa gaaggtgaac aacatcctat gtacgccgcc ttagagactc
    23281 tgggatttga tgtcggcaca cttggcaacc acgaatttaa ctacggcctt gcctacctcg
    23341 aaaaagtaat tcgtacagcc aacatgcctc tagtcaatgc taatgtcctc gatccgacta
    23401 caaaagactt tctctacacc ccttatacca ttgtgaaaaa gacattcaca gacaccgagg
    23461 gtaaaaaggt tacactaaat gtcggtgtta cagggattgt tccccctcaa attcttaact
    23521 gggacaaagc ttatctagaa ggaaaagtga ttgttcgtga tgcagttgaa gccgttcgcg
    23581 atattatccc aaccatgcga gaaaatggcg cagacatcgt tctggtcctt tcccactcag
    23641 gtatcgggga tgaccaatat gaagtcggcg aggaaaatgt cggttaccaa atcgccagct
    23701 tgtcaggagt tgatgcggtt atcacaggtc actcccacgc agaattccca ggaactgccg
    23761 aaaaaccaag cttctatgct aaatactctg gcgtggatga tacaaatggt aaaatcaacg
    23821 gtactcctgt caccatggcg ggtaaatacg gtgatcacct cggcgtcatc gacctcaatc
    23881 tggtctttaa agatggcaaa tggacaacta cttctagcaa agcggctatt cgtaaaatcg
    23941 atacaaaatc atctgtagca gatggtcgca tcatcgacct tgctaaagaa gcccataacg
    24001 aaaccatcaa ctatgtgcgc caacaagtcg gtgagacaac tgctccaatc aacagcttct
    24061 ttgcccttgt tcaagatgat ccttctgtac agattgttaa caatgcacag atttggtatg
    24121 caaaacaaca gttggcgggt acttcagaag ctaatctacc aatcctatct gcggctgcac
    24181 catttaaggc tggtactcga ggcgatgctt cagcctatac agacattcct gctggcccaa
    24241 tcgccatcaa gaacgtagca gacctttacc tctacgacaa cgttgtagcc atcttaaaag
    24301 tcaacggtgc ccaactcaaa gaatggttgg aaatgtccgc agggcaattc aaccaagttg
    24361 acccaagctc aacagagcca caaaacctag tcaacacaga tttccgcact tacaattttg
    24421 acgttattga cggagtaact tatcagtacg atattacgca accaaataaa tacgatcgta
    24481 acggtaaggt agtcaacgaa actgccagca gagttcggaa tctgcaatac aatgggcaag
    24541 atgtgacagc tgatcaagaa ttcatcgtgg tgaccaacaa ctaccgtgca aacggtactt
    24601 tccctggggt ccgcgaagct tctgtcaatt acttgctcaa tctggaaaac cgccaagcca
    24661 tcatcaatta tattatcgct gagaaagtta tcaaccctac agcagacaat aactggacct
    24721 ttacagatag catcaaggga ctcgacctcc gcttcttgac agcagatcgt gcaaaagaat
    24781 tggtagctaa tcaagaaggg ctggtttacc tccaagcctc agctgaaaac gaaggatttg
    24841 gcgaattcaa atttgtctac actgagccaa aagtagcgac tccagatgaa cagcagtcag
    24901 atcaaggtaa cacaggtcaa gatattgtac tcgaatctgg tcaacgaatt actcttccag
    24961 cggtaaatcc acctgctcct gctccacaac ataaattggc tagtccacat tcacaagcga
    25021 gcaccaagac actccctgca acaggagaag ctacttctat gcttagtctg ctcggtctca
    25081 ctttgattgg ttttgtcggc gcatggacta agaaaaaaga acattaaaat aatcaagagc
    25141 ttgttttttc aagctctttt ttaccctcat acactctcta gataaattaa tcaaagcata
    25201 taaaaatagc tcgaagacgt actcgagcta tttttatctt tatatcgcta ctcccaataa
    25261 atcggtacat agcgggtttc tttgggactt tttactgtat aggttcccaa atctacccag
    25321 tttgattggt tcgccaaccg ctgttctact ctaaactcct gacaaaggat cgttgtttct
    25381 aagcgaatgt ttccatactt ccttgcaaaa acaaggtaag aaacgctttc tgaagcaaag
    25441 acgagattcc ccaaagtcga ttttctagga cgactaaact ccataattct accttcttct
    25501 ccaccataaa tcgaagaaat cagattttcg ctcataatgg tttcatgata ggttgtttcg
    25561 agagaagtac gactatcaga cgcttgaaaa cctcgactag acgtactcga tagttgacga
    25621 ataaaagcct ttctcacttc atgattttca aactcatagg tctgctctgt tgcttgatta
    25681 tacaaaacca attccgagat aatctgatag ctagtccctg gaactctacc gaattgatta
    25741 agagcaacaa agactatcaa gaaaaatcca agagcagcaa aaataggggt actcaatctt
    25801 ttcatgggca atctccatca catatctcct caatcttcgc taattgcaac gaccattgag
    25861 ctatgaacga atccatcacg atcctgttta ttcgaatcgt tcagagcgat acattcacta
    25921 tttgctaacg tgtgttcgag actattttct ctctttagat gataaatcat tcactttatt
    25981 acaataatag tatatcaata tgatttttaa aaaacaacta atcaggtatg aaaatgtaaa
    26041 ccagatcatg taaaatatga ccaaataact gcaatcacga tggctggcaa catattggcc
    26101 acacgaatct ttttgtccca gatgatattg acaccgacac agaagatgag ggcggagccg
    26161 ataaaggaga gattggcaag ggcctgatcc gtcatcaaag gttcaatcag gcgggccaaa
    26221 agagtcacag agccttgaaa gagaaaaacg ggtactgctg aaaagcctgc tcccttgtcc
    26281 aaactcgagg tcataatcaa aatgataata aagtccagaa tactcttaac ggccaacaag
    26341 cgataatcac ccgtcaatcc atcttggatg gctcctagta tggccatagc accgatacag
    26401 acggtcaaag aagctgtcag gaagcatcta agaattgacc atcttggctg tttcctgacc
    26461 tttctcggag ccagataccc aagcgctcaa accaaccttc gatatgcagt acttccccaa
    26521 tcaaggtccc cagggctaga ctaagaacca aaagcatgct attttgactt ttgagttgac
    26581 catcaacgac tgtcagcagt ccttctaaac tacctgaaat tcctagaaat aaaacagcta
    26641 gcccgctcgc caccgttagt cctgactgat gccgttcttt cagtaaatgg ccaaaccaag
    26701 atcctagcaa acctgctagg gcaatggcca ccgtattgat aatggttcct aatgcgaaca
    26761 tgatcctcct ttatgattca ctatcatctg gcaatagata ataaactttt ccatcttcaa
    26821 ccaacaattt cacattctcc ccataattgt taacagacaa tcgcttacta gattgatagg
    26881 aaacagtttc ttgttctgaa attagcttaa ctgtccgttt tcgaacatca atgtaacgaa
    26941 tggccttatt tagctctcca gccgctttac caattccaaa acctgctgct gcaccaacaa
    27001 ggcctccaag aaagcctact ccagcagcaa catgggcatt cttatccaac tcttttgcaa
    27061 tatctgcaac tactttcata ggaccctctt gggtacgtct attgcgctta gtgtcaattt
    27121 ggaaaaattt tatcttatct ttgtgccaat tggctatctt ttcacctaga ctattacaat
    27181 tgtttagata gtactcatac acatagtaac tactatcctc tgacgccact ccttgcgaga
    27241 agactagatc tagcttacta agttccgcta ataatctcaa caaggtttgt tgatagaata
    27301 aattagtact taacttatca attaactgtt catacttttc aaaagataat ccttttccct
    27361 tagtgatact ctcttcaata gctagattga cctgttctag taacttggtt acttcagcac
    27421 ggaagttagc acactccatc aactttagtc ttgcaagttc agcattttcg acatagtctg
    27481 ctgaaaattt tgatagttga gaaatattaa ctatcaaagc gcgaacagtc cctttaaatt
    27541 cccttacttg aaaatcctta acatcgttca acgtttcttt cattgaatca agctgaccat
    27601 tgatttcagc catataatac tgaccaacta ccatagcagc gacctgcata gctcctgaaa
    27661 cagcagctgc agcttttgct ccttttgcaa tttcaacttg agcaggagtt agtattgcat
    27721 gagccttaaa ttttccttcc gcacctttaa caaaagcacg atacttatcc gtttcccctt
    27781 tgattttgtg caaatcgcct atcggcaaat ccactttaaa caatccctta ggaatattag
    27841 gctgtccggc tttcttaaga tgatgtttaa gtgctggttg aactatcttt tcaacatgag
    27901 ccaaaatatt cggattcgta atttcatatc tctgatatgc tgatgaatcc aactcactca
    27961 ttttctgaat gagagcaaag caatcatccg aaacaatcaa ttctcggtgt tccttttgtt
    28021 ctaattcact catctcccga ttctccttac ctcaacaatt ccgtcgcgta actaatagca
    28081 gaaagggcgt agaggggagc cgtttctgct cgcaagatac gaggaccaag acctgtcttg
    28141 accgctcctg ctccttcaaa tgcggctact tcctcaggcg acacgccacc ttctggaccg
    28201 acgatgacaa ggactgattg gccgatttct aagccagaca agattttcac caggttggct
    28261 tgttcgccct cttttgcggc ctcttcgtag gctaagataa tctggtcaaa gcctgctagt
    28321 tgagctagga aatccacttt tttctcaaat aaacgaacag ctggaatgcg attgcgtttg
    28381 ctctgctcgg ctgcgccttg ggcaattttt tctaactttt ctgctttttt gcctaatttt
    28441 ttaccgtccc atttgaccac ggaccagtcc gctggaaagg cccagagggc tgccatgcct
    28501 aactccgttg ccttttgggc gacaaattcg agcttatctc ccttgggaaa gcccatggct
    28561 atagtgacgg aaacaggtaa ttcgacattg tctgacagtt cttcgatgat ttcaacagat
    28621 tggttctggc tatctaccac gcgcgccaag cgtttaatcc cgtcgtcaaa gactaggaca
    28681 atctgttcat ctgcctgcaa gcgcatgaca gaaaacatat gcttggcggt gtccttatcg
    28741 cttatctgga acacgccctg cggtgctctg ccattgataa aatactgctg catcctagcc
    28801 tccaatcaca cctgagcggt cagccgtctt cttaaagaca cagcaattcc actcgccctg
    28861 aatcatatgg gtctcaagga agaaaccagc cgcctccgca gatgctcgca ccatgtccca
    28921 cttatccgcg atgatgccgc tcataatcag gtagccttcg tccttgacca gacggtaggc
    28981 atcctcggtc agatggatga gaatgtccgc caagatgttg gcaacgataa cttctgcttt
    29041 aatatctacg ccacgaagca aatcgcccgc tgcaacatga atattgctag tattagcgtt
    29101 gaggtcaata ttttcctgag ccacacgcac cgcaacttca tccaagtcat aggcataaat
    29161 ctccttggca cctaggaggg agctagcaat agaaaggaca cctgaacctg tgccgacatc
    29221 aatgactgtc tcgccaccac gcaagacctg agaaagagca aagaggctca tcttggtcgt
    29281 cgggtgggtt cctgtaccga aagccatgcc tggatccaag cggataatct tctccccagc
    29341 tgtcgcctca tagtcggtcc aagacggtac aatggtcaaa tcgtggctaa tgcgggctgg
    29401 ctcatagtat ttcttccagt tgtccgccca gtcttcctct gccaattcct gactttctag
    29461 gctgacctga cctgtttcca agccaaagcc tgtcaaatcc gccaagcgag tcgccaagtc
    29521 tgccttaact tcctcaatat ctacagtatc aggataatag gccgtaatca caatcatatc
    29581 cgactgttcc acctcgggat agagttcacc aaaacggtcc tcctgcccca catagtcagc
    29641 cgagtcgcta atagccaccc cctgactgcc cgtttcaatc atgagatttg aaactgcttc
    29701 ttctgcatca cgatgcacgt gaatcgttaa ttcttgccat gagttcatgt ttgttgttcc
    29761 ttttcttttc tatgcgtcaa tcactgtatt attgtaccga tttctagcct attttacaag
    29821 ttaatgttac gcgatggagc cctaataata ctttttcgaa aattttctaa tcgcactgcg
    29881 aataaacatg tgtaggaaag tttgagacgg tggttccaag tttctgaacg gaggtgatgc
    29941 ccatgaaagt ggacagcaaa tctgctagaa aggctagctc atgccagatg gtaagtaaac
    30001 ttactcatct ggcaacgaca aatcctataa gatttgtcta gggcacgcac tagctcgagt
    30061 tttgaaaaat atcgtttcaa aactctcacg tcggagaact ctggatttat atttatcaag
    30121 tctacttgtt gaaatgctga gcttgataat cgtcatcatt ttagctattc gagataaaaa
    30181 ataaccgtca gcaaactttg acacagttct gacggttatt taatctaacg aactgaacca
    30241 tcgttatggt cctgtcaggg cgttgagctg gaactcaacg ccttttctat ttttataata
    30301 gcaaaagttt cgcctgcagt caagcaagaa agaaaacgga atgtcaaata gaatctatta
    30361 ccctcaattc tataccaact gcaccgtact gttctaattg ttctcgtgaa tagtaatctt
    30421 ccatatcttc gggtttcgcg cctctgatac tgctttgcgt atacccacaa gacaataaat
    30481 ccagttcttt gtataactga gcaaaattat caaatatgtg aagatctaat acttgcacct
    30541 cgatcgtttg tgtctgattt tctgtacaat aaaaacggat gcgatctcca atttgtatat
    30601 gcttgcgctt ctcgtcataa agtcgtagct caatggtctt ctgaccagac ttcatcattt
    30661 caaaaggttt tggagccagt aacatttcat gaaccaactc aatacctcgg atatgggtaa
    30721 aattctccct ccaacaagtc tgctctgcct ttttcaaaag cctctccgtc ccaagaaatc
    30781 gcaaaatctt gggcttctgg gtccaccaaa aaatccatga ggtcatctaa accttggtcg
    30841 gcggtatctt tgagaaagcc tgcgaagtag gtcagaaact ccgctgaaaa gcccttcttg
    30901 gcgtcaaatg gcagagcgac caggtagtct tcctcgtcaa agcgagactt gtcctggttg
    30961 tagaaaagga catactcctc taggaaaata tcctcagaag acgcattacc ttcgtcgtcc
    31021 accgtttcaa tcccagccac attttgcgct tccaagataa aagccaactc aactgcgtgg
    31081 tttttcttgt cccagttgag ctcgtaatcg taggtaaaat gtttgtccag tgccgcttcc
    31141 agcacatcta aaaatccatg ttttgccatg tcagtcctct ttcgattttg tgttataata
    31201 agtgtaccat aatcagctaa caaaagaaac gaaaggaagc ctatgccagc caatctcgcc
    31261 cttcgtatgc ggcccaaatc cattgatgaa gtcatcggtc aggaacacct ggtcggtcct
    31321 ggaaagatta tccgtcgcat gattgatgcc aatatgctgt cgtccatgat tctctacggt
    31381 ccgccaggga ttggtaagac ctcgattgcg tccgccattg ctggtacgac caagtatgcc
    31441 tttcgaacct ttaatgccac gacagacaac caaaaacgcc tgcaggaaat cgctgaagaa
    31501 gctaaatttt ctggtggcct ggtcctcatg ctcgatgaaa tccaccgcct caacaagact
    31561 aaacaggact tcctgcttcc tcttttggaa aacggcaata tcatcatgat tggagcaacg
    31621 acggaaaatc ccttcttctc aattttgcct gccattcgca gtcgggtgca gatttttgaa
    31681 ttacaacctt tgaaaaccag tcacatccga caggccttgg aactggctct gacagacagc
    31741 gaacgtggtt ttgattttcc cgtcaccatt gaacctgagg ccctggattt tctggcaaat
    31801 gcgacaaacg gtgaccttcg tgccgcttac aattctctag aactggctgt gctttcgacc
    31861 aaggaaagtg acgacggtag ccgccacatt gatctggacg ccgtggaaaa tagcctgcaa
    31921 aagtcctaca tcagcatgga caagaacggc gatgcccgct acgatatcct ctccgcccta
    31981 caaaaatcca ttcgaggtag cgatgtcaat gccagcctcc actacgccgc tcgtttgatt
    32041 gaggccgaag atctgcctag tctggcccgt cgcttgacgg tcattgccta cgaagacatc
    32101 ggcttggcca atccagaggc tcagattcat acggtgacgg cccttgaagc cgcccagaaa
    32161 attggctttc cagaggcacg cattttgatt gccaatgtgg tgattgattt ggctctttct
    32221 cccaagtcca attctgccta tctggctatg gatgcagctc tggctgattt gcggaaaaac
    32281 ggccatctgc ccattccaaa tcatcttcgg gatggccact atgctggcag caaggagctg
    32341 ggaaatgcta ttggctacca gtatccgcat gcctatcctg aaaaatgggt ggaccagcaa
    32401 tacctgcccg ataagttact agctgcggac tacttcaccg ccaacgacac tggaaaatac
    32461 gagcgtgcct tgggcatgac acaagaaaag ataaaaaatt tgaaaaaaaa tagacgccaa
    32521 aatcc
//
LOCUS       DBNFEG010000007        27776 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.007, whole genome shotgun sequence.
ACCESSION   DBNFEG010000007 DBNFEG010000000
VERSION     DBNFEG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 27776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27776
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.007"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..453
                     /locus_tag="AC1PNG_000190"
     CDS             <1..453
                     /locus_tag="AC1PNG_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HIP6700732.1"
                     /translation="GLHKLNDIHFSHKKSSLVFDDILDDRINSDLLESSLYYDKKGVR
                     SRLEILSYLYAGFTKPNLVVRKAKNFPIKGSKLRWSYLVEFYIDDIRLGEFFIDNYRS
                     GHSNEFIGVSIFEKSEKDYTVNQTKFTILSIYETDIVSGNVVVLFTRM"
     gene            695..5599
                     /locus_tag="AC1PNG_000191"
     CDS             695..5599
                     /locus_tag="AC1PNG_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0018262 - isopeptide cross-linking
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SspB-related isopeptide-forming adhesin"
                     /protein_id="HIP6700733.1"
                     /translation="MTKQCHHHFLVNQEKAEKHVFRKSKKYRTLCSVALGTMVTAVVA
                     WGGQVAQADEVVTPSQGKTVQLTENPATNLPEAQPTPVVEQTNSLALTGQSDGAITVT
                     VPHDVVTQAVNQATAEGVTTIQDKPMDLGNTTSASDTSKQLDKAEVDAARQAETITQV
                     TNTYKADKATYEQDKTRVEQGNAALAASHKEATQVGKALNSSVDSTASKIKGQDQSAN
                     VTVTTQTVTSGDGSTVSGYQDYTSAVAAIDKQNKDNLSEYESKKKDADAFVAKNLVTQ
                     KENEAGLAKAKADNEAIDKRNQAGQKAVDDENKAGQAAVDAYNKDQQKLVTDREAEIA
                     SITKRNKEKEEAAKTENDAIDAYNAKEMERYKRDLAEISKGEEGYISQALAQALNLNN
                     GEPQARHSADTRNPNRIVAKGDAMLGGYSKILDSTGFFVYDTFKTGEILSFTYQNLQN
                     ARFDGKKITKVAYDITNLVSPAGTNAVQLVVPNDPTEGFIAYRNDGTGNWRTDKMEFR
                     VKAKYFLEDGSQVSFTKEKPGVFTHSSLNHNDIGLEYVKDSSGKFVPINGSTIQVTNE
                     GLARSLGSNRASDLKLPEEWDTSYSKYAYKGAIVSTVTSGNTYTVTFGQGDMPQNVGL
                     SYWFALNTLPVARTVKPYSPKPHVTPKLEPIPEPIKVVPKTFTPKTFTPEPPVSFKEK
                     PLEKVTQPSLTLTKVSLPKDPTPEPLPKAPQVPTVHYHDYRLTTTPEIVKEVIITDQV
                     NLHDKTVAKDSTVIYPLTVDVFSPNRAKTTSLTFEDYLPAGYAFDKEKTQAENRNYTL
                     SFDAAKNFVTLTAKDALLQEINQDLTKSYQLMAPKLYGSLQNDGATYSNSYQLLINKG
                     TPNAYTVTSNVVTVRTPGDGTTTSRIEPQKKNDNEDGVLINDTVVALGTTNHYRLTWD
                     LDQYKGDRSSKETIARGFFFVDDYPEEVLDLVDNGTGITTPDGKTVSGITVKAYASLS
                     EAPKDLQDKLAHAKISPKGAFQVFMPNDNQAFYDQYVKTGTSLNLLTKMTVRDSLYGQ
                     TKTYRNKAYQVDFGNGYETDEVTNTLVSPSPKKQNLNKDKVDINGKPMVVGSQNYYTL
                     SWDLDQYRGIKADKTQIAKGFYFVDDYPEEALLPDEASIQLVTTDGKAVSGVTVKTYT
                     SLSEAPKPLQVALSKRKIAPKGAFQVFMAEDPQAFYDSYVTQGQNITIVTPMTVRESM
                     LNSGKSYDNVAYQVDFGQAYETNTVTNHVPKVNPHKSNTNIKGVSIDGKTVLPNTINY
                     YKIVLDYSQYKDLVVTDDVLTKGFYMVDDYPEEALILNSDGVQVMDKAGNLVKGISVK
                     AYASLSEAPKVVQDAMAKRQFTPKGAIQVLSADDPKAFYEAYVKTGQTLVVPLPMTIK
                     NELTKTGGKYENTAYQIDFGLAYVTETVVNNVPQLDPQKDVVIDLSQKEKSLDGKEVA
                     LNQVFNYRLVGALIPGNRATPLIEYRFDDDYDESHDDYNGVYKAYTLVDVTLKDGTVL
                     PKGTEVTKYTLQQVDTSKGTVTISFDQEFLEKLAEESEFQADVYLQMKRIASGEVENT
                     VLHTVNGYTISSNTVKTTTPEPELPTPTQPTPPQPPIPTQEPPVPASVLPNTGESQSL
                     LALVGGGLLLGLAYGLSKRKMEDN"
     gene            5600..5791
                     /locus_tag="AC1PNG_000192"
     CDS             5600..5791
                     /locus_tag="AC1PNG_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HIP6700734.1"
                     /translation="MEPKNIYTRDSDQDGLTDAQELALGTNPFSSDTDSDGLSDLEEV
                     QQGLNPIQQRKERSYGLEL"
     gene            5775..6326
                     /locus_tag="AC1PNG_000193"
     CDS             5775..6326
                     /locus_tag="AC1PNG_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700735.1"
                     /translation="MAWNYDTISRTLSEMARENYEDMVKAFLAMELSIKNKSLLDTLY
                     QDFMGIDDLSLVSDDLRLRADGYQEQLQEEVTDLLDKLYRTGEGASFIMEVIASNNIS
                     ESLAQYEVLNEEDYSSLTLETLQDIIQKELSLTSQDYFGDVTYLALQKDLLDQKSHFL
                     QHYVTTLMDKLPQEKDQRDLVLD"
     gene            6377..12310
                     /locus_tag="AC1PNG_000194"
     CDS             6377..12310
                     /locus_tag="AC1PNG_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003084104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HIP6700736.1"
                     /translation="MTQEQLLEMLRARLPRDQILLESFLRYQAVHFDEDWDSLIQNFT
                     TQRGVVTSPVQVVRFETEVSAFVKASPFNEATDLSSYTQTFGQAGLKKLPQLNSDEKA
                     LVVEVALFNLATRFQLLDQEGAYQSISVASLLDKGKAANLVNVYRVANNLSDRISRDI
                     EQFLLTYEPELISTQETVEEREGAAEEKIVPELPQDITFREEGGIVIASLEEDLSQLD
                     LRTGQTEHLPAYEHLNLSQKFEILSHFDQVRNSRPKLPNLRRGEFDHEMEMTPIYEDN
                     ELLTYLEADGTVYDLQRPLTPQEEVILTEMGQTILAENTEKLTNLGIDLADVDEQQRG
                     ILIDAAGRFHLKNADLALLGGYPKATVTQLALATELLQMGLSHDKVEFFLTSQLDLED
                     ARPIAYAFLHEELTLEEARTFESAKQSQPDISFREWREHLSQTEPEIIVTQEKLPNPI
                     VEEALKRYPIASIVTYKGQEFQVMAIEDSGVNNLIRVELQNDFTDVIEQNPVLFLRTL
                     EDITQALHVPSVEEKEEVEEPSQELDLFSFMDMEESQEAVSQVTASVSSNKREAKQEE
                     ALSEDELEPEVTETPPATDFHFPEDLTDFYPKTTRDKVETNVAAVRLVKSLESEHRQA
                     TPSEQELLAKYVGWGGLANEFFDEYNQKFSKEREELKTLVTEKEYSDMKQSSLTAYYT
                     DPLLIREMWNKLERDGFTGGRVLDPSMGTGNFFAAMPKHLRENSELYGVELDTITGAI
                     AKHLHPNSHIEVKGFETIAFNDNSFDLVLSNVPFANIRIADSRYDKPYMIHDYFVKKS
                     LDLVHDGGQVAIISSTGTMDKRTENILQDIRETTVFLGGVRLPDSAFKAIAGTTVTTD
                     MLFFQKHMDKGYVADDLAFSGSIRYDKDDRIWLNPYFDGEYNSQVLGSYEVRNFNGGT
                     LSVRGTSDHLLADVQTALKQVKAPRVVDNYDIFITPDVIKRQVIDTSVPPEIRESLDQ
                     YSFGYKDSTVYYRDNKGIRVGTKTEEISYYVDDEGTFKAWDTKHSQKQIDRFNALEVT
                     DSTALDVYVTEEATKRGQFMGYFKKTVFYEAPLSEKEVARIKGMVDIRNAYQEVIAIQ
                     RYYDYDRDEFNHLLGHLNRTYDTFVKRFGYVNSAVNRNLFDSDDKYSLLASLEDESLD
                     SSGKTVIYTKSLAFEKALVRPEKEVTAVSSALDALNSSLADGRGVDLAYMMSIYQTDS
                     KATLIEELGDAIIPDPERYLNDREVVYVSRQDFLSGDVVTKLEVVDLLIKADNSDFPW
                     VYYQGLLEDVKPPRVTLADIDYRIGSRWIPLAVYGKFAQETFMGQTFDLTDQEVSTVL
                     EVSPIDGTMSYQSKFAFRYSTATDRSLGVPGSRYDSGRKIFENLLNSNQPTITKQVED
                     GDKKKHVTDVEKTTVLRAKETELQELFQDFVASYPEVQQMIEETYNSLYNRTVSKVYD
                     GSHLTIDGLAQNISLRPHQKNAIQRIVEEKRALLAHEVGSGKTLTMLGAGFKLKELGM
                     VHKPLYVVPSSLTAQFGQEIMKFFPTKNVYVTTKKDFAKAKRKQFVSRIITGDYDAIV
                     IGDSQFEKIPMSHEKQVTYIQDKLQQLRDIKQGSDSDYTVKEAERSIKGLEHQLEELQ
                     KLERDTFIEFENLGIDFLFVDEAHHFKNIRPITGLGNVAGITNTTSKKNVDMEMKVRQ
                     DDYRNVVFATGTPVSNSISELYTMMSYIQPDVLELYQVSNFDSWVGAFGNIENAMELA
                     PTGDKYQPKKRFKKFVNLPELMRIYKETADIQTSDMLDLPVPEAKVIAVESELTEAQK
                     YYLEELVDRSDAIKSGSVDPSVDNMLKITGEARKLAIDMRLIDPAYTLSDNQKILQVV
                     DNVERIYREGNLEKATQMIFSDIGTPKNKEEGFDVYNELKDLLMDRGIPKEAIAFVHD
                     ANTDDKKNSLSRKVNSGEVRILMASTEKGGTGLNVQSRMKAVHHLDVPWRPSDVGRIL
                     RTFKIKKNVEVTDNGKDNF"
     gene            12291..12665
                     /locus_tag="AC1PNG_000195"
     CDS             12291..12665
                     /locus_tag="AC1PNG_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HIP6700737.1"
                     /translation="MAKTIFEEMGGKYERQGDYLIPCLTVPAEEEQPIGIWGQRHLDY
                     LKHHCKVTYTNLLTSGRLNAYLADIDRQAQERFERLIEGMKQAQGITEQLKAENALEW
                     TGCLNNIRACAREIVEKEIIFA"
     gene            13032..14951
                     /gene="tet(O)"
                     /locus_tag="AC1PNG_000196"
     CDS             13032..14951
                     /gene="tet(O)"
                     /locus_tag="AC1PNG_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(O)"
                     /protein_id="HIP6700738.1"
                     /translation="MKIINLGILAHVDAGKTTLTESLLYTSGAIAEPGSVDKGTTRTD
                     TMNLERQRGITIQTAVTSFQWEDVKVNIIDTPGHMDFLAEVYRSLSVLDGAVLLVSAK
                     DGIQAQTRILFHALQTMKIPTIFFINKIDQEGIDLPMVYQEMKAKLSSEIIVKQKVGQ
                     HPHINVTDNDDMEQWDAVIMGNDELLEKYMSGKPFKMSELEQEENRRFQNGTLFPVYH
                     GSAKNNLGIRQLIEVIASKFYSSTPEGQSELCGQVFKIEYSEKRRRFVYVRIYSGTLH
                     LRDVIKISEKEKIKITEMCVPTNGELYSSDTACSGDIVILPNDVLQLNSILGNEMLLP
                     QRKFIENPLPMLQTTIAVKKSEQREILLGALTEISDGDPLLKYYVDTTTHEIILSFLG
                     NVQMEVICAILEEKYHVEAEIKEPTVIYMERPLRKAEYTIHIEVPPNPFWASVGLSIE
                     PLPIGSGVQYESRVSLGYLNQSFQNAVMEGVLYGCEQGLYGWKVTDCKICFEYGLYYS
                     PVSTPADFRLLSPIVLEQALKKAGTELLEPYLHFEIYAPQEYLSRAYHDAPRYCADIV
                     STQVKNDEVILKGEIPARCIQEYRNDLTYFTNGQGVCLTELKGYQPAIGKFICQPRRP
                     NSRIDKVRHMFHKLA"
     gene            15003..15192
                     /locus_tag="AC1PNG_000197"
                     /pseudo
     CDS             15003..15192
                     /locus_tag="AC1PNG_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876549.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
     gene            15712..16176
                     /locus_tag="AC1PNG_000198"
     CDS             15712..16176
                     /locus_tag="AC1PNG_000198"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIP6700739.1"
                     /translation="MWNCPIRISAAAGKPTRDIWTSCLSPVQGCLLEVVREQYADFYR
                     DRERWRYLQKLDARNSLLSLDGFTDSEGNPLDFIADEAADIAETVVNAVMVDRLKAAL
                     PLLSDSEQELIQAIFFDGLSEREVGARLGITQSVVNKRKARILRKLRKIIEN"
     gene            16271..16696
                     /locus_tag="AC1PNG_000199"
     CDS             16271..16696
                     /locus_tag="AC1PNG_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIP6700740.1"
                     /translation="MAYNHGREDRKWRIWKEAEEKLLRECGVDEVTIEQIRIADRADF
                     NSNRRFYRWTNDVAEYLEDMADRERQAEVNTVAELLDEIESENLYQVLVTVDGRTLKI
                     VLLKMQGYSTKEIAPLVHLTTGAIYARLDHLRKKLRKIL"
     gene            16780..17253
                     /locus_tag="AC1PNG_000200"
     CDS             16780..17253
                     /locus_tag="AC1PNG_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIP6700741.1"
                     /translation="MAYKAKAYTLREESTESGIRYFISFKDGQGEHHELEVSEQLFFE
                     FRQMERRNRNLLQWDERHREFSEVWDETLNRRALKLPKSIEEQMIEAERAELLCKAVD
                     RLPEIQRRRFLLYYEYEFNFYQIAAMEHCTASAIQKSVAVAKKKVKAEMRKYLQP"
     gene            17407..18431
                     /locus_tag="AC1PNG_000201"
                     /pseudo
     CDS             17407..18431
                     /locus_tag="AC1PNG_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521636.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            18684..18950
                     /locus_tag="AC1PNG_000202"
     CDS             18684..18950
                     /locus_tag="AC1PNG_000202"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700742.1"
                     /translation="MPDVPSGLPYGRSYALLSYALRLRIKERRHDGYSSCEGVSQKHR
                     HHRHDRHAGDKGDSYGNRQGASQNCRHHRHRCHPKGSYPPKKGR"
     gene            18957..19922
                     /gene="mobV"
                     /locus_tag="AC1PNG_000203"
     CDS             18957..19922
                     /gene="mobV"
                     /locus_tag="AC1PNG_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HIP6700743.1"
                     /translation="MPYAILRFQKRKAGGVAACERHNERKKEAYKSNPDIDMERSKNN
                     YHLVAPPKYTYKKEINRKVAEAGCRTRKDSVMMVETLITASPEFMNQLPPEEQKAYFT
                     MALDFISERVGEQNILSAVVHMDERTPHMHLCFVPITPDNKLSAKTILGNQKSLSEWQ
                     TAYHERMSSRWNQLERGQSSMETKRKHVPTWLYKLGGRLDKQYGEIVSALSDINAFNA
                     GKKRDKALELVAAWLPEVEKFSKEIGRQQAYIDSLKEQIGQEADYAGRMRDEKYEQEL
                     KVQKANQRIFELQRTNEQMGRLLSKIPPEVLEELQRTGRNKSRER"
     gene            19930..20784
                     /locus_tag="AC1PNG_000204"
     CDS             19930..20784
                     /locus_tag="AC1PNG_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIP6700744.1"
                     /translation="MKKQDFKVLKTKDLYPFPDNPFHVVEDETLSELAESIKEFGIVT
                     PIITRPKEDGNGYEVIAGQRRVRASELAGINTVPAFVLPLDRDRAIITLVDSNLQREN
                     ILPSERAFAYRMKSEAMKRQGFRTDLTSSQVVTKLRTDDKVAQGFGVGRMTVQRFIRL
                     TELIPPILQMVDEGKIALTPAVELSFLKKDEQENLFATMESEEATPSLSQAQRMKSMS
                     QSGQLDMDMIFSIMTEEKGNQKETLKINTSKLKKYFPKDTTPKQMEETIIRLLERELQ
                     RKRSRDSR"
     gene            20861..21091
                     /locus_tag="AC1PNG_000205"
     CDS             20861..21091
                     /locus_tag="AC1PNG_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HIP6700745.1"
                     /translation="MKEIQYEIVKEIAVLSASDSGYTKEINLISWNGREPKYDIRSFS
                     PNREKCGKGITLNADEAAALLEALQKEVNSGD"
     gene            21162..21353
                     /locus_tag="AC1PNG_000206"
     CDS             21162..21353
                     /locus_tag="AC1PNG_000206"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700746.1"
                     /translation="MGEDRKTTGRRKRIVPKGQVQMIISREYIGTQTVAEAFVPIISE
                     DIRKKIAEGDTFDNEGLSA"
     gene            21407..23290
                     /locus_tag="AC1PNG_000207"
     CDS             21407..23290
                     /locus_tag="AC1PNG_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003084608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIP6700747.1"
                     /translation="MAGIRTENKIYEVGIYCRLSKDDGTDNESASIATQKSILTDYVK
                     RQGWHLAKTYVDDGYSGTNFQRPSFQNMIKDIENGLINCVITKDLSRLGRNYLDCGLY
                     LEVFFPEHNVRYIAVNDGVDTLNKSAMDITPFRNILNEMYSADVSVKIKSAYRARFQQ
                     GKFMGTTAPYGYVKDPADHNHLLIDDKVAHVVREIFDLALAGNGIAKIRKHINKQHIL
                     RPAAYAVEQGATGYERYFEDNEENRYIWSENSVRGILRSPIYAGNLAGYKRIAANMKS
                     KKRPSKLPEEWEVIPDTHEGIVTQEEFDTVQQLMTSRRREQNAGGFENIFSGVIKCAD
                     CGYAMRAASANRRKRPDIIDCVQYTCNNYGRYGNVMCTAHSIEARDLFNAVLADINRF
                     ADMAVNDEKAVRAIERRLTETDQSRAKSLEKEKKKLNKRLAELDRLFSSLYEDKVMER
                     ITERNFEMMSGKYQKEQLEIEARLKEVTETLNDSYEKSQGVRDFLSLIRNYQGLKELD
                     ATIINALIDKILVSEREKLADGTVRQEIKIYYKFIGFVGELHITPTKRWTALKPKNCT
                     VCGVEYVPSSGISKYCPACAKRIQREKSNESKRRSRERNRRACIELSAKNDRLIWCSA
                     MVG"
     gene            23257..24216
                     /locus_tag="AC1PNG_000208"
                     /pseudo
     CDS             23257..24216
                     /locus_tag="AC1PNG_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24286..24585
                     /locus_tag="AC1PNG_000209"
     CDS             24286..24585
                     /locus_tag="AC1PNG_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5962 family protein"
                     /protein_id="HIP6700748.1"
                     /translation="MTQTVEELRYQLEEWLAQGFTSPEDRANYQALKEQYEDETLDYS
                     FSKREIIGQLEVIITTRENDFPDLDEVTKGEYLDLVEQLDNLDKGQADYYRKQLA"
     gene            24600..24890
                     /locus_tag="AC1PNG_000210"
     CDS             24600..24890
                     /locus_tag="AC1PNG_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5966 family protein"
                     /protein_id="HIP6700749.1"
                     /translation="MIEQIIQSLFIIVATGLILLVLYQIAKMLGSLFVIGLIGFLAFT
                     EVYGIYLFFTERYLYVEDLATNGILSFTTFYIIFNLLLVFGLVRKVVRSRMA"
     gene            25001..25639
                     /locus_tag="AC1PNG_000211"
     CDS             25001..25639
                     /locus_tag="AC1PNG_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIP6700750.1"
                     /translation="MLNKMKARLLIGLGGLAVTSFMVMMGYTIGSQSVTNHTQKQIQS
                     EAQKLLTKEKEKEKASVLSDELVKEFLTQYYTKEKLGENNNRIKPYMTDSAFKEEVAR
                     QEDSINQVYKDYMLDYRFESANIYVNAEENEAFAEVSYQVVYVSDVSDKAHKSTQTET
                     RTLKLSYAKLSDKLFVNQVTIWNGKLEDLKESSDEVNSSIPKIQGTTTSETN"
     gene            25679..26767
                     /locus_tag="AC1PNG_000212"
     CDS             25679..26767
                     /locus_tag="AC1PNG_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIP6700751.1"
                     /translation="MTRIEKVKQKAILEVAESLGYSFKRLSGQVYEHPEHDSFRIFAD
                     TNTFKWFSRDVQGDVIDFVQLMTGVSFKKALSYLETGDFEQAKIVEETYQPFRYYLRE
                     EPFDQARTYLNDIRGLSDDTINAFGRQGLLAQAHYQTKTFQESVLVFKSYNHLGQLEG
                     ASLQGLVKDNQRHDRGYLKKIMKDSHGYIGMSFDIGKPKRLIFCESVIDMMSYYQLHQ
                     KQLSDVRLVSMEGLKLSVIAYQTLRLAAEEQGKLEFLDTVKPSRLTHYLHAIQETTTF
                     FQTHPGLLTLAVDNDEAGRDFCQKLSEKGLPTETDCPPLQELETKADWNDIVKYQNNY
                     SLKDVIQSAKLQVIRSNPPPRKNTALEL"
     gene            26821..27048
                     /locus_tag="AC1PNG_000213"
     CDS             26821..27048
                     /locus_tag="AC1PNG_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5965 family protein"
                     /protein_id="HIP6700752.1"
                     /translation="MSVIERLAERVARQEEKVAKETEKLETYRSQLQTAMYQTFIKRQ
                     QNSPWTFDEALTQAFGQDEQELKPSDTRNEE"
     gene            27052..27447
                     /locus_tag="AC1PNG_000214"
     CDS             27052..27447
                     /locus_tag="AC1PNG_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5945 family protein"
                     /protein_id="HIP6700753.1"
                     /translation="MTKDWTFDQPLDDNTPTSSSEERAKIAALFHKENQEGTEDVDYV
                     AAFEMEQQKAKEKSVSQPKTQEQSTPKEVSSSNITNDYKQHLADVMAQNNEDILQSQK
                     KIEELHQLIDDKNKQNKKLQAISAAIDDL"
     gene            27491..27775
                     /locus_tag="AC1PNG_000215"
     CDS             27491..27775
                     /locus_tag="AC1PNG_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700754.1"
                     /translation="MKLFKKQTHNQDSFKRLIHRLSGMSEADLHKVNQLLDVVFDSTS
                     QTTNDVKPPKDLQKEPTLDETITEAKNKLKTEQLEKRIEQFRQAKKPKSE"
BASE COUNT         8759 a         5522 c         6493 g         7002 t
ORIGIN      
        1 ggacttcata aactaaacga tatacatttt agccataaaa aatcttctct agtttttgat
       61 gatattttag acgaccggat taactctgac ttattagaat cttcgttgta ttatgataaa
      121 aaaggggtta ggtcacgttt agaaattttg tcgtatttat atgctggctt tacaaagcct
      181 aatcttgtcg ttagaaaagc aaagaatttc cccatcaaag gtagtaaatt aagatggagt
      241 tatttggtgg aattttacat tgatgacatc cgtctaggtg agttttttat tgataactat
      301 cgaagcggtc attctaatga atttattggt gttagtattt ttgaaaaatc tgaaaaagat
      361 tatactgtga atcaaacaaa gtttacaata ctttcaattt atgaaacaga tattgtgtca
      421 ggaaatgtag tggttctttt cactagaatg tagttctctt agttatatat aatatcatgt
      481 tgttatcgaa ttggggcgtg ctaatcacat ctgggagggt ctgatgggtc aggccccctt
      541 ttttataagc aattttcaga ggttatcggt agtcctaccg atagcttttt tctttgtcct
      601 tctttgatgt gataccaaaa tcaagacgtc ttttttatga ttaggagaga gtgacagatt
      661 gttactactt atcaaaagaa aagaaggact ttttatgacc aaacaatgtc atcatcactt
      721 tttagtcaat caggaaaaag cagaaaagca tgtcttccgt aaaagtaaga agtaccgtac
      781 gctctgttcg gtagctcttg gaaccatggt gacagctgtt gtcgcttggg gtggccaagt
      841 agcacaagct gacgaagtgg taacaccctc gcaaggtaaa accgttcagc taacagaaaa
      901 tcctgctacc aacttaccag aagctcagcc aactccagtt gttgaacaga cgaatagtct
      961 tgcgttaact ggtcaatcag atggggctat cacggttacg gttcctcatg atgttgtgac
     1021 acaggctgtc aatcaagcaa ctgctgaagg ggttacgacc attcaggata agcctatgga
     1081 tttgggaaac accacctctg cgagtgatac cagcaagcaa ttagataaag cggaagtgga
     1141 tgctgctagg caggctgaaa ccatcactca ggtgaccaat acctataaag cggataaagc
     1201 gacttatgaa caagataaaa cacgtgttga acaagggaat gctgcgttgg ctgctagtca
     1261 taaagaagcc actcaagtag gaaaggcctt gaatagttca gtggatagca cagcttcaaa
     1321 aatcaagggc caagatcagt cggctaatgt gaccgttaca actcaaactg taacatcagg
     1381 agatggttca acggtttcag gctatcagga ttatacctct gcggtagctg caattgataa
     1441 gcaaaataag gacaatctat ctgaatacga atcaaagaaa aaagatgcag atgcctttgt
     1501 cgcaaagaac ttagtgactc aaaaggaaaa tgaagcgggt cttgctaaag caaaagctga
     1561 taatgaagca attgataaac gcaatcaagc cggacaaaaa gcagtggatg atgaaaacaa
     1621 ggcaggtcaa gctgctgttg atgcctataa taaagaccaa cagaaattag tcacagaccg
     1681 tgaagctgag attgcttcta tcacgaaacg caataaggaa aaagaagaag ccgccaagac
     1741 agaaaatgac gccattgatg cctacaacgc caaagaaatg gagcgctata aacgtgattt
     1801 agctgagatt tcaaaaggtg aggaaggcta catctcacaa gctcttgctc aggctctcaa
     1861 tttgaataat ggagaaccac aagcccgtca ttccgcggat acaagaaacc caaatcgtat
     1921 cgttgctaag ggtgacgcta tgcttggtgg gtattctaaa atcctagatt cgacaggatt
     1981 ctttgtctat gacaccttta aaacaggaga aatcctttcc tttacctatc aaaatcttca
     2041 aaatgctcgc tttgatggta agaaaatcac aaaagtagcc tatgatatta ctaaccttgt
     2101 ttctccagct ggaactaatg ccgtgcagtt agtcgtacca aatgacccaa cagaaggctt
     2161 tatcgcctac cgtaatgatg ggacaggtaa ttggcgcaca gacaagatgg agtttcgtgt
     2221 caaggccaag tatttcttag aagatggctc acaagtgagc ttcaccaagg aaaaaccagg
     2281 tgtctttact cattcctcac tcaaccacaa tgacatcggt ttagagtatg tgaaagactc
     2341 atcaggcaag tttgtcccta tcaatggctc aaccattcaa gtgactaacg aaggtctagc
     2401 tcgttcactt gggtcaaatc gtgccagtga tttgaagctt cctgaagaat gggatacgtc
     2461 ttacagtaag tatgcttaca aaggagcgat agtctcaacg gttacatcag gcaacactta
     2521 tacagtgacc tttggtcaag gggatatgcc ccaaaatgtt ggtctctctt attggtttgc
     2581 cttaaacacc ttacctgtcg cacgaaccgt caaaccttat agtccaaaac cacatgtgac
     2641 accaaagtta gagccaattc cagaaccgat taaggttgtg ccaaaaacct ttaccccaaa
     2701 gacctttacg ccagagccac ctgtgagctt taaggaaaaa ccactggaga aagtcactca
     2761 gcctagtttg acactcacta aggtgtcctt gcctaaagac cctacgccag agccgttacc
     2821 aaaagcacca caagtgccaa cggttcatta ccacgactat cgtctgacaa cgactcctga
     2881 aattgttaag gaagttatca ttaccgacca agtgaacctt catgacaaaa cggtcgcaaa
     2941 agattcaacg gtcatttacc ctttaaccgt agacgttttt tctccaaatc gagccaaaac
     3001 cactagcctt acatttgaag attaccttcc agcaggctat gcctttgaca aggaaaaaac
     3061 gcaggcagaa aatagaaatt ataccctcag ctttgatgct gctaagaact ttgtgacctt
     3121 gaccgccaag gatgccttac ttcaagagat caatcaagac ctcaccaagt cttaccaact
     3181 tatggctccg aaactttatg gtagccttca aaatgatggg gcaacctatt ccaacagtta
     3241 tcagctcctc atcaataagg gaaccccaaa tgcctataca gtgacttcca atgtcgtgac
     3301 ggttcgtacg cctggtgatg gaacaaccac tagccgtatt gagccgcaga agaaaaatga
     3361 caatgaagat ggtgtgctca ttaacgatac ggtagtggct ttaggaacaa ctaatcatta
     3421 ccgtttgacc tgggatttag atcaatataa aggcgataga tcttctaaag agacaattgc
     3481 tcgtggtttt ttctttgtag atgactaccc agaagaagtg ttggatttag tagacaatgg
     3541 aacaggtatc accactcctg atggcaaaac cgtatcaggg attactgtca aggcttatgc
     3601 gtcgctgtca gaagctccaa aagaccttca agataaactc gctcatgcta agatttctcc
     3661 aaaaggggct ttccaagtct ttatgcctaa tgacaatcag gctttttatg accagtatgt
     3721 taaaacagga acctctttga accttctcac caaaatgacg gtcagagata gtctttatgg
     3781 tcagacgaag acttatcgaa acaaagccta ccaagtggat tttggcaatg gctatgaaac
     3841 cgatgaggtg acgaataccc ttgtaagccc atcacctaag aaacaaaact tgaataagga
     3901 taaggtggac attaacggaa aaccgatggt agtgggttca caaaactact ataccttgtc
     3961 atgggacttg gaccaatacc gaggcattaa ggctgataag actcaaattg caaaaggttt
     4021 ctactttgtg gatgattatc ccgaagaagc tctattacca gatgaagcaa gtattcaact
     4081 tgtgacaact gacggcaaag cagtgtcagg tgtgacggta aaaacttata cgagcttatc
     4141 agaagcccct aaacccctac aagtagctct ttcaaaacgc aagattgcgc ctaaaggagc
     4201 cttccaagtc tttatggcag aagatccgca agccttctat gattcttatg taacgcaagg
     4261 tcaaaatatc accatcgtta ctcctatgac tgtccgtgag tctatgctta attcaggaaa
     4321 gtcttatgac aatgtagctt atcaagtaga ctttggacaa gcctatgaga ccaatacggt
     4381 aacgaatcac gtgccaaaag taaaccctca taagtccaat accaatataa agggcgtgtc
     4441 tattgatggg aagaccgttc ttccaaatac cattaattac tacaaaattg ttctggatta
     4501 cagtcagtac aaggatttgg tagtgacaga tgatgttctt acaaaaggtt tttacatggt
     4561 ggatgattac cctgaagaag cgcttatcct aaactcagat ggcgttcaag tgatggataa
     4621 ggcaggaaat cttgtcaaag ggatttcggt gaaagcctat gcctcattgt cagaagcgcc
     4681 taaagtggtt caagacgcta tggccaaacg ccagttcaca cctaaagggg ccattcaagt
     4741 tttaagcgct gatgatccaa aggcatttta tgaggcttat gttaaaactg gtcaaacctt
     4801 agtggtgccc cttccaatga ctattaagaa tgagctgaca aaaactggtg ggaagtatga
     4861 aaacaccgct tatcagattg actttggttt ggcttatgtg acagagactg tggttaataa
     4921 tgtgccacaa ctagacccac aaaaagatgt ggtgattgat ttgtcacaaa aggagaaaag
     4981 tcttgatgga aaagaagttg ctttgaatca ggtctttaac taccgcttgg tgggagcact
     5041 tattcctggt aatcgtgcga caccactcat cgagtaccgc tttgacgatg attacgatga
     5101 aagccatgac gactataatg gtgtttacaa ggcttatacc ttagtagatg tgactctaaa
     5161 agatggaacg gttttaccaa aaggaacaga agtgactaag tacacgctac aacaggtcga
     5221 cacgtcaaaa ggaacggtta ccatcagttt tgatcaggaa ttccttgaaa aactagcaga
     5281 agaatccgag tttcaggctg atgtttactt gcagatgaaa cgaattgctt caggtgaagt
     5341 agaaaatacc gtattacata ctgtcaatgg ctacaccatc agttcaaaca cggttaaaac
     5401 aactactcct gaaccagagc taccaactcc gactcaacca accccacccc aaccacctat
     5461 tccaacacaa gaaccaccag ttccagcaag tgtcttacca aatacaggag agagtcaatc
     5521 tcttttggcg ctcgttggtg gaggccttct tttaggccta gcttacggac tttctaaacg
     5581 caaaatggag gacaactaaa tggaacctaa aaacatttac acaagagatt cagaccaaga
     5641 tggtctaacg gatgctcaag agttagcttt agggaccaat ccttttagca gtgatacgga
     5701 tagtgacgga ctgtcagacc ttgaagaagt ccaacaaggc ttgaatccaa ttcaacagcg
     5761 taaggagaga agctatggct tggaattatg atacaatttc tagaaccctt tctgagatgg
     5821 cacgagaaaa ctatgaagac atggttaagg cctttttagc catggaattg agtatcaaaa
     5881 ataagtcact tcttgatacg ctctatcaag actttatggg cattgatgac ttatctttag
     5941 ttagtgatga cctgcgccta agagcagacg gttatcagga acagcttcaa gaagaagtga
     6001 cggacctctt ggataaactc tatcgaacag gtgagggagc tagtttcatt atggaagtga
     6061 ttgcgtccaa caacatttct gagtcccttg ctcagtatga ggttttgaat gaggaggatt
     6121 actcgtctct caccttagag accttgcagg acattatcca aaaggagttg tctttaacga
     6181 gtcaagatta ttttggagat gtgacctatc tcgccttgca gaaggatttg ttagaccaga
     6241 aaagccattt cttacaacat tatgtgacaa ctttgatgga taagttgcca caagaaaaag
     6301 accaaagaga cttggtccta gactaaagca gtgggctgag aatacctctc agctcctttt
     6361 ttaattggag gaacatatga ctcaagaaca attacttgag atgttgcggg cgagattgcc
     6421 acgtgaccag attcttttgg aatccttttt acgctatcaa gcagtccatt ttgatgagga
     6481 ttgggatagc cttatccaaa attttacaac tcaaagagga gtagtcacct cacctgttca
     6541 agtggttcgc tttgaaacag aggtttctgc ttttgtcaag gcgagcccgt ttaatgaagc
     6601 gacagaccta agcagctaca cacagacctt tggtcaagct ggattaaaga agttacctca
     6661 attaaacagt gatgagaaag ccttggtggt tgaggtagcc ctctttaacc tagccactcg
     6721 ctttcagctc ttagatcaag aaggcgccta tcagtccatt tcggtagctt ctcttttgga
     6781 taaaggcaaa gctgctaatt tggtcaatgt ctatcgagtg gccaataatt tgtctgaccg
     6841 tattagccgt gatattgaac agtttcttct cacttatgaa cctgaactaa taagcaccca
     6901 ggaaacggta gaggaaagag agggtgcagc tgaagaaaaa atagttccag aacttcctca
     6961 agacatcacc tttcgtgaag aagggggtat cgtcattgca agtcttgagg aagatttatc
     7021 acaactagat ttacgaacag ggcaaacaga acatttacca gcttatgaac atctaaactt
     7081 gtcccaaaaa tttgagatat taagtcactt tgaccaagtg cgaaatagcc gtccaaaact
     7141 accaaacttg agacgtgggg agtttgacca tgaaatggaa atgacaccaa tctatgagga
     7201 caatgagctc ttaacctatt tagaagctga tgggacagtc tatgacttgc aacgtcctct
     7261 aacacctcaa gaagaagtaa tcttaactga aatgggtcaa accattttgg ctgaaaatac
     7321 cgaaaaattg accaatttag ggatagactt agctgacgtt gacgagcagc aacgtgggat
     7381 actgatagat gctgcagggc gttttcattt gaagaatgcg gacctagcct tattaggggg
     7441 ctatcctaaa gcaaccgtta ctcaattagc ccttgcgaca gaattgcttc agatgggatt
     7501 gtcacacgat aaagtagaat ttttcttgac cagtcagcta gacttagaag acgcaaggcc
     7561 cattgcctac gccttcttgc atgaagaact cactcttgaa gaagctagaa cctttgaaag
     7621 cgccaaacag tcccaaccag atatatcttt tagagaatgg cgagaacacc tatcgcaaac
     7681 agaacctgaa atcattgtga ctcaagaaaa actaccaaat cctatcgtag aagaagccct
     7741 taaacgctat ccgattgctt cgattgtgac ttataagggg caagagtttc aggtaatggc
     7801 cattgaggac tcaggcgtta ataacctgat acgggttgaa ctacaaaatg atttcacaga
     7861 tgtgattgag caaaatccag ttcttttctt acggacacta gaggacatca ctcaggctct
     7921 tcatgttcct tctgtcgaag aaaaagagga agtggaagaa cctagccaag aattagacct
     7981 cttttctttt atggatatgg aagaatctca agaggcggtt tctcaagtga cggcttctgt
     8041 atcatcaaat aagagagagg ctaaacaaga agaagcattg tcagaagatg agttggaacc
     8101 agaagtcact gagacgcccc cagccactga ttttcacttc ccagaagatc taacagactt
     8161 ttaccctaag acaacaagag ataaggttga aaccaacgtg gctgctgttc gtttggtgaa
     8221 aagtcttgaa tctgaacacc gtcaagccac acctagtgaa caagaactcc ttgctaaata
     8281 cgtgggctgg ggaggcttag ctaacgagtt ctttgacgag tacaaccaaa agttttctaa
     8341 ggaacgtgag gaattaaaaa cgctagtcac agagaaagag tattctgaca tgaaacagtc
     8401 ctctctgacc gcttactaca cagaccctct cttgattcgt gagatgtgga ataaactaga
     8461 acgagatggc tttacaggtg gaagagtgct agatccttcg atgggaacgg gaaatttctt
     8521 tgcggcaatg cctaaacacc tgagagaaaa tagtgagttg tatggtgtag agctagacac
     8581 gattacaggg gctattgcca aacaccttca tccaaatagc catattgaag taaagggctt
     8641 tgagaccatt gcctttaatg acaatagctt tgatttggtt ctctcaaatg tgccttttgc
     8701 caatatcagg attgcggata gtcgctatga caaaccctat atgattcatg attactttgt
     8761 caaaaaatca ttagatttgg tgcatgatgg tgggcaagtc gctatcattt cctccacagg
     8821 aaccatggat aaacggacag aaaatattct ccaggacatt cgtgagacga ctgtttttct
     8881 tggtggtgtg cgtttaccag atagcgcctt taaggccatt gcaggaacga ctgtcacaac
     8941 agacatgttg ttcttccaga aacatatgga taagggatat gtggcagatg atttagcttt
     9001 ttcaggttct attcgctatg acaaggatga ccgtatttgg ttgaatcctt actttgatgg
     9061 agaatacaac agtcaggttc ttgggagcta tgaggttaga aactttaacg gagggacact
     9121 ttctgtgaga ggaacttctg atcatttgct agcagatgtt cagactgctc ttaaacaggt
     9181 taaagcccct agagtggtgg ataactatga tatcttcatc actcctgatg tgataaaaag
     9241 acaagtcatt gatacctctg ttccgcctga aattagagaa agtctagacc aatatagttt
     9301 tggttataaa gattctacgg tttactaccg agataataag ggaatccgtg ttgggactaa
     9361 gaccgaggag attagctatt atgtggatga tgaggggacc tttaaggctt gggacactaa
     9421 gcattctcaa aagcaaattg accgttttaa tgctttagaa gtgactgata gcaccgctct
     9481 tgatgtgtat gtgaccgaag aagccaccaa acgtgggcag tttatgggct atttcaaaaa
     9541 gacggttttc tatgaagctc ccttgtcaga gaaagaagtg gcacggatta aagggatggt
     9601 tgatattcgg aacgcctacc aagaggtgat tgccattcag cgctactatg attacgacag
     9661 agatgagttt aaccacttgt taggacatct caatcgcacc tatgatactt ttgtgaaacg
     9721 ctttggctat gtgaatagtg ctgtgaaccg taacctcttt gatagtgatg ataagtattc
     9781 ccttcttgcg agtttagaag atgaaagtct tgactcaagt gggaagacag ttatctatac
     9841 caagtcgctt gcctttgaaa aagccctagt tcgtcctgaa aaggaagtga cggcagtttc
     9901 ttctgccctt gatgctctca attctagtct agcagatggt cgtggggtcg atttggctta
     9961 tatgatgtct atttatcaga cggattctaa agcgactttg attgaggaat taggtgacgc
    10021 tattattcca gacccagaac gctatctaaa cgatagggaa gtggtctatg tgtctcggca
    10081 ggatttctta tcaggagatg tggtgactaa gttagaggtt gtggacttac tcattaaagc
    10141 agacaactct gattttcctt gggtctatta tcaaggtctc ttagaagacg tgaaaccacc
    10201 acgtgtgacc ttagctgata ttgattatcg gattggctca cgttggattc ctctagccgt
    10261 ttatgggaaa tttgcccaag aaacctttat gggacaaact tttgacctga ctgaccaaga
    10321 agtatcaact gttcttgaag tcagtccaat tgatggcaca atgtcctacc aatccaagtt
    10381 tgcctttagg tattctaccg caacggatag aagtttaggt gtgcctgggt cacgctatga
    10441 tagtggccga aaaatctttg aaaacctgct taattccaat caaccaacca ttaccaaaca
    10501 agtggaagat ggggataaga aaaagcatgt gacggacgtc gagaagacaa cggttcttcg
    10561 tgctaaggaa actgagcttc aagaactctt ccaagacttt gtggcaagtt acccagaggt
    10621 gcaacagatg attgaagaaa cctataatag cctttataat cgtacagttt caaaagtcta
    10681 tgatggcagt catttaacca ttgatgggct tgcccagaat atttccttac gccctcacca
    10741 gaagaatgcc attcaacgga ttgtggaaga aaaacgagcg cttctagctc atgaagtggg
    10801 ttcaggtaag actttaacca tgcttggggc aggcttcaaa ctgaaagaac tcggaatggt
    10861 gcataaacct ctttatgtgg tgccgtcaag tctgaccgct cagtttggcc aagaaattat
    10921 gaagttcttc ccaacgaaga atgtctatgt gacaaccaag aaagattttg cgaaagccaa
    10981 acgtaagcag tttgtctcac ggattatcac aggggactac gatgccatcg tcattgggga
    11041 ttcccagttt gagaaaattc ccatgagtca tgaaaagcaa gtgacctata ttcaagataa
    11101 gttacaacaa cttcgtgaca tcaagcaagg tagtgatagt gattatactg ttaaagaagc
    11161 agagcgttct attaagggct tagagcacca attggaagaa cttcaaaagt tggaacgtga
    11221 tacctttatt gaatttgaaa atctagggat tgactttctc tttgtggacg aggctcacca
    11281 ttttaagaat atcagaccca ttacaggcct tgggaatgtg gctggaatta ccaacacgac
    11341 ttctaaaaag aacgtggata tggaaatgaa ggtcagacag gatgattatc gtaacgtggt
    11401 ttttgcgaca ggaactccag tctctaactc tattagtgaa ctctatacca tgatgagcta
    11461 tattcaacca gatgtcttag aactgtatca agtctcaaac tttgactcat gggttggagc
    11521 ttttgggaat atcgaaaacg ctatggaact agcaccgaca ggggataaat accaacctaa
    11581 gaaacgcttt aagaagtttg tgaacctgcc tgaactcatg cggatttata aggaaacagc
    11641 tgatattcag acctcagata tgcttgattt acctgtacct gaagctaaag ttattgcggt
    11701 ggagagcgaa ttgacagaag ctcagaaata ctaccttgaa gagttggttg accgttcaga
    11761 cgctatcaag tcaggcagcg ttgaccctag tgtcgataac atgttaaaga taactgggga
    11821 ggctagaaaa ctagctattg acatgcgtct tattgatcca gcctatacct tatctgacaa
    11881 tcagaagatt ctgcaagtgg tggataacgt ggaacggatt taccgtgagg gcaatctcga
    11941 gaaagctacc cagatgattt tctcagatat tggaactcct aagaataagg aagagggctt
    12001 tgacgtttac aatgaattga aagacctatt aatggataga gggattccca aagaagcaat
    12061 tgcctttgtc catgacgcca ataccgatga taagaaaaat tctctgtcac gcaaagtcaa
    12121 tagtggggaa gtacggattc tcatggcttc tactgaaaaa ggtggaacag gattaaacgt
    12181 ccaatcacgt atgaaagctg ttcatcattt agacgttccc tggcgtccct ctgatgtagg
    12241 acggattttg cggacattca aaataaaaaa gaatgtggag gtaacagaca atggcaaaga
    12301 caatttttga ggaaatgggc ggcaaatacg aaaggcaagg cgattattta ataccgtgct
    12361 taactgtacc cgccgaagaa gaacagccga taggcatctg gggacagcgg catttggatt
    12421 atctgaagca tcactgcaaa gttacataca ccaatcttct tacaagcggc agacttaacg
    12481 cttaccttgc cgacattgac agacaggcac aggaacgctt tgaaaggctc atagagggta
    12541 tgaaacaggc acagggcata acggaacagc taaaggcaga aaacgcctta gaatggacag
    12601 gatgcctcaa taacataagg gcttgtgcga gggagattgt ggaaaaggaa attatttttg
    12661 cataaacaga tgattagtgg cagggggaaa tcctgccgct ttttctgctt tagtttgtca
    12721 gcttgacaaa taaagggtta aggaatataa ttagattcag tattatacaa ggagttaata
    12781 aatatgcggc aaggtattct taaataaact gtcaatttga tagtgggaac aaaaagtagc
    12841 agtcccgttt cacttttaat atggggctta gttttttgta cccagtttaa gaatactttt
    12901 atcatgtaat tttatatgcc cgaaaacata taagtgtttt ggggctattg gagttattta
    12961 cccagtgata ggagtattta tcactgggta tttttatgcc cttttttggg tgttgatagg
    13021 aggaaaatca catgaaaata attaacttag gcattctggc tcacgttgac gcaggaaaga
    13081 caacattaac ggagagttta ttgtatacca gtggtgcaat tgcagaacca gggagcgtag
    13141 ataaaggcac aacaaggaca gatacaatga atttggagcg tcaaagggga atcactatcc
    13201 agacagcagt gacatctttt cagtgggagg atgtaaaagt caacattata gatacgccag
    13261 gccatatgga ttttttggcg gaagtatacc gttctttatc cgtattagac ggagcagtat
    13321 tattagtttc tgcaaaggat ggcatacagg cacagacccg tatactgttt catgcactac
    13381 agacaatgaa gattccgaca atttttttca tcaataaaat tgaccaagag gggattgatt
    13441 tgccaatggt atatcaagaa atgaaagcaa agctttcttc ggaaattata gtgaagcaaa
    13501 aggttgggca gcatccccat ataaatgtaa cggacaatga cgatatggaa cagtgggatg
    13561 cggtaattat gggaaacgat gaactattag agaaatatat gtcagggaaa ccgtttaaaa
    13621 tgtcagaact ggaacaggaa gaaaacagga gattccaaaa cggaacgtta tttcccgttt
    13681 atcacggaag tgctaaaaac aatctgggga ttcggcagct tatagaagtg attgccagta
    13741 agttttattc atcaacgcct gaaggtcaat ctgaactatg cgggcaggtt tttaagattg
    13801 aatattcaga gaaaaggcgg cgttttgttt atgtgcgtat atatagcgga acattgcatt
    13861 tgagggatgt tattaaaata tctgaaaaag agaaaataaa aatcacagag atgtgtgttc
    13921 cgacaaacgg tgaattatat tcatccgata cagcctgctc tggtgatatt gtaattttac
    13981 caaatgatgt tttgcagcta aacagtattt tggggaacga aatgctgttg ccgcagagaa
    14041 aatttattga aaatcctctc cctatgctcc aaacaacgat tgcagtaaag aaatctgaac
    14101 agcgggaaat attgcttggg gcacttacag aaatttcaga tggcgaccct cttttaaaat
    14161 attatgtgga tactacaacg catgagatta tactttcttt tttggggaat gtgcagatgg
    14221 aagtcatttg tgccatcctt gaggaaaaat accatgtgga ggcagaaata aaagagccta
    14281 ctgttatata tatggaaaga ccgcttagaa aagcagaata taccatccac atagaagtcc
    14341 cgccaaatcc tttctgggct tctgtcgggt tgtccataga gccgctccct attggaagcg
    14401 gagtgcagta tgaaagcaga gtttcacttg gatatttaaa ccaatcgttc caaaatgcgg
    14461 ttatggaggg ggttctttat ggctgcgagc aggggctgta tggatggaaa gtgacagact
    14521 gtaaaatctg ttttgaatat ggattgtatt atagtcctgt aagtaccccc gcagactttc
    14581 ggctgctttc ccctatcgta ttggagcagg ctttaaaaaa agcagggaca gaactattag
    14641 agccatatct ccactttgaa atttatgcac cgcaggaata tctctcacgg gcgtatcatg
    14701 atgccccaag gtattgtgca gatattgtaa gtactcaggt aaagaatgac gaggtcattc
    14761 tgaaaggaga aatccctgcc agatgtattc aagaatacag gaacgattta acttatttca
    14821 caaatgggca gggagtctgc ttgacagagt taaaaggata ccagccagct attggtaaat
    14881 ttatttgcca accccgccgc ccgaatagcc gtatagataa ggttcggcat atgttccaca
    14941 agttagctta acagcttgca aaagtcatat aaaatgagat ttgaaaggat tagagactaa
    15001 ttatgatgaa atgcgaatgg atattgtgtc ctgtttgtgg gagcaaaacc cgtaataaaa
    15061 ttaggaagga cactgttttg gagaattatc ccctttattg tccaaaatgc agacaagaaa
    15121 gcttgattaa agttgacaac ttgaagataa ctgtcatcaa agagccagac gcttaagacg
    15181 cagagccgat gaaattgtgg aacaattcac gaatcatcgg ctctttttgt ttcgtatttg
    15241 aaagaacaaa ctcaccaaat aaaaaaaacg atattcgggt gggttatttt gttataccct
    15301 aaattaccct ctgaatttgt ttttaaattt ggagggattt ttttatgttc ttttttcggg
    15361 cggttattca tctgctcata cgaagtaaaa tttatcaata catagcatat cagagaatgg
    15421 caggaaacca gttaaaaaaa tttctgccag tgcagcggta cttctcacct tgaaagtgga
    15481 agtacaatct ccatacaaca gaatttgtat ttcccataac cgtgaaggtc acagagcctt
    15541 tgcggttttt cttttgtcgt tttttgttgg aaagtgccgc agggctgttt ctggtgtgcg
    15601 ttgccgctcc ccgcctctcc atctggattt ttacatttca aaaattcaga ttggaggtat
    15661 tagcggtgaa atacgcacca agaaaagtat atatcaaaga aagcggcggc tatgtggaac
    15721 tgtcctatac ggatttctgc cgccgcaggc aagccgacaa gggatatatg gacaagctgt
    15781 ttatcccccg tccaaggttg tctgcttgaa gtcgtaaggg agcaatatgc ggacttctac
    15841 cgtgacaggg agcggtggcg ttacctgcaa aaattagatg cgaggaacag cctgctatct
    15901 ctggacggat ttacggacag cgaggggaat cctctggact ttatcgctga tgaagcggcg
    15961 gacattgcgg aaaccgttgt caatgctgtt atggtggaca ggctgaaagc cgccctgcct
    16021 ttgttgtcgg atagtgagca ggagttgata caggcaatct tttttgacgg actttccgag
    16081 cgtgaagtcg gggcgaggtt aggcataacc cagagcgttg tgaacaaacg caaagccaga
    16141 atcctaagaa aactaagaaa gataatagaa aattaaaatt taaggcgttc agcccccttg
    16201 ttttttcctt tgggaaaatg agggggcttt tctctgccct ctcaatcaat tctgattgga
    16261 ggaagaaaga atggcataca accacggacg ggaggacagg aaatggcgta tctggaaaga
    16321 agcggaggaa aagctgctgc gtgagtgcgg cgttgatgaa gtgaccattg agcagatacg
    16381 catagcggac agggcagact tcaattccaa caggcggttt taccgatgga cgaatgacgt
    16441 tgcggaatac cttgaggaca tggcagacag ggagcggcaa gcggaggtga atacagttgc
    16501 ggagttactg gacgagattg agagcgaaaa tctctatcaa gtattagtca cggtggacgg
    16561 gcgtaccttg aaaatcgtcc tgctgaaaat gcaggggtat tccacaaagg agattgcccc
    16621 acttgtgcat ttgacgactg gtgccatcta tgcgaggtta gaccatctgc ggaagaagct
    16681 tcggaaaatt ttgtaagcgt ctaatacatc ccattattct gcgggctact gggtggggag
    16741 tggaatctcc ccgcttattt ttggcaggag gaaaacggga tggcatataa agctaaggcg
    16801 tacacgcttc gggaggaatc cacggaaagc ggtataaggt attttatcag ttttaaggac
    16861 gggcaggggg aacaccacga actggaagta tccgagcagt tattctttga atttcggcag
    16921 atggaacgca ggaacaggaa tcttctccaa tgggacgagc ggcacaggga gttttcggaa
    16981 gtatgggacg aaacgctgaa caggcgggcg ttaaagctgc ctaagagcat tgaggaacaa
    17041 atgattgaag cagaacgggc ggaactgctc tgtaaggcgg ttgacagact gccagagata
    17101 caaaggcgac gtttcctgct ctactacgag tatgagttca atttttacca aatcgccgct
    17161 atggagcatt gcaccgcttc tgcaatacag aaatccgttg ctgttgcaaa gaagaaagta
    17221 aaagcggaaa tgaggaaata tctccaacca tgaccgacac cgcccgaaaa agaaatctgt
    17281 tttttatttg tgtgggattg gcggtattac atactctcac ttttttcgtg ggagtaaatc
    17341 tatttttaag gaggtcaacg cctatgtgca acaaaaacaa gataactgcc cgaaaggagg
    17401 aaacccatgc agaagttaca gacagtcaac gccgaaacgc tcctttatga gccgcttgag
    17461 aagccgtcct ttgtggtgga cagccttatc cccacaggct tgtcgctgtt ctgcggctca
    17521 cagaagatag gcaagagctg gctcatgctg aagctgtgct tatgcgtgtc gcagggaatc
    17581 cccttatggg acatgacgac aatggagggc gatgtgcttt acctctgcct tgaggacacg
    17641 ttctgccgca tacaggacag gctgttccgt ttgacggacg aagcgagcgg gcggcttcat
    17701 tttgccgtgg caagctgcaa gctgtcagac ggtcttatcg tgcagcttga ggattatctg
    17761 aaagactatc cagacagcag gctcattgtc attgatacct tgcagaaagt ccgtacagct
    17821 tcaaaagaca acgcctatgc aagcgactat ggggacatct ccctaatcaa agattttgcc
    17881 gacaggcact ctctggcggt cattgtcgta caccacatcc gaaagcagaa tgacagcgat
    17941 gtgttcaaca aggtgtctgg gacgacaggc ttaacgggga gtgcggacgc tacctttgtt
    18001 ctggaaaagg agaaacgggc atctgacacc gccaagctgt atgtaacggg cagggacacg
    18061 ccctatcagg aatacacgct ccgtttccgt gattgcagtt gggaacttgt ggagaggaaa
    18121 acgcaggagc agcttgcgaa agaaacgata ccagatgtcc tttttcggtt ggtggatttt
    18181 atgagggata aggaagaatg ggcaggcacg gcaacggagc tgttagccgc tatgggggaa
    18241 acggaaacca tacccacggt gattacgaaa tggctgaatg aatatcacac cacattttta
    18301 agcgagaacc gtatctgcta ccagtacagc cgcaggaaag acggcaggca gattgccctt
    18361 gcaaggaggg cgggtgacag cggtgacggc ggtgacagcg atattgggat accccctgtt
    18421 actgtcattg acgcttaaag ccatgtaaag ttggcggctc tgccctgcgg gtgacagcgg
    18481 tgacggcagt gacagtgatt ttaggatacc ctgccgctgt catccctacg ggagaacacc
    18541 ccgcaaacgc aaaatgcagg cgtgggattc acccgccctt ttcgggcgtg taaaatcacc
    18601 cctgcggtca gaaaaatctc tccgattttt ccgactgcgt ttacagggtg taacacacta
    18661 cactttgccc tgcaaagagc cgtgtgccag acgttccctc tggactccct tatggcaggt
    18721 cttacgccct gctatcctac gccttgcggc ttcggataaa agaacggcgg catgacggtt
    18781 acagctcttg cgagggagta tcccaaaaac accgtcacca tcgtcatgac cgtcatgcag
    18841 gggataaggg tgacagttac ggcaatcggc agggggcatc ccaaaactgc cgtcaccacc
    18901 gtcaccgctg tcacccaaag ggcagttacc cgccgaagaa aggaagatga tggattatgc
    18961 cctatgcaat cctgcgtttc cagaaacgca aagcgggcgg cgttgcggct tgcgaacgcc
    19021 acaacgagcg gaagaaagaa gcctacaaaa gcaacccaga tatcgacatg gaacgctcta
    19081 aaaataacta tcatcttgtc gcaccgccaa agtacaccta taagaaagag attaaccgaa
    19141 aggtagccga agcagggtgc aggacaagga aagacagcgt gatgatggtg gaaacgctca
    19201 tcacggcttc accagaattt atgaaccagt taccgcccga agaacagaaa gcgtatttta
    19261 cgatggctct ggatttcatt tcggagcgtg tcggggagca gaatatcctc tccgcagtcg
    19321 tccacatgga cgagagaacg ccccatatgc acctctgctt tgtgccgatt acaccagaca
    19381 ataagctgtc cgccaaaaca attttaggca atcaaaagag cctgtcggag tggcagaccg
    19441 cctaccatga gcggatgtcc tcacgttgga atcagcttga gagaggtcaa tcttcgatgg
    19501 aaaccaagcg gaaacacgtc cccacatggc tctataagtt gggcggcagg cttgataagc
    19561 agtatgggga aatcgtgtct gccctgtccg acatcaacgc ctttaacgca gggaaaaagc
    19621 gtgacaaggc tctggaactg gttgccgcat ggctgcccga agtggagaaa ttctctaagg
    19681 aaatcggcag acagcaggcg tatatcgaca gcttaaagga gcaaatcggg caggaagccg
    19741 actatgcagg gcgtatgcgt gatgaaaagt acgagcagga attaaaggtg cagaaagcca
    19801 accagaggat atttgaattg cagagaacca acgagcagat ggggcggctg ctctcaaaga
    19861 taccgccaga agtgttggaa gaattacaga gaacaggcag aaacaaatca agagaaaggt
    19921 aaaaagtgaa tgaagaaaca ggattttaag gtgttaaaga ccaaagattt atacccgttt
    19981 cccgacaatc cgtttcatgt tgtggaggat gaaacgctgt cagagttagc ggaaagcatc
    20041 aaggaatttg gcattgtcac gccgataatc acacgcccga aagaggacgg gaacggttat
    20101 gaagtgattg cggggcagcg gcgtgtccgt gcgtccgaac ttgcaggtat aaataccgtg
    20161 cctgcatttg tcctgccctt agaccgtgac cgagccatca tcacccttgt agacagtaat
    20221 ttacagcgtg aaaatatcct gccatcggag cgggcgtttg cctacaggat gaaatccgaa
    20281 gccatgaagc ggcagggttt ccgcacagac ttaacctcgt cacaagttgt gacgaagttg
    20341 cggacggacg acaaagtggc acagggcttc ggcgtgggca ggatgaccgt ccagcgtttt
    20401 atccgcctga cggaactgat accgccgatt ttgcagatgg tggacgaggg gaaaatcgcc
    20461 ctcacacctg cggtggaact gtcctttttg aagaaagacg agcaggaaaa tctctttgcc
    20521 acgatggaga gcgaagaagc aacgccctca ctctcacagg cacagcggat gaaaagcatg
    20581 agccagagcg ggcagcttga catggatatg atattttcca ttatgacgga ggaaaaggga
    20641 aaccagaaag aaaccttgaa aatcaacaca agcaaactga aaaaatactt tccgaaggac
    20701 acaacgccga agcagatgga ggaaaccatc atccgacttc tggaacgtga gttgcagaga
    20761 aaacggagca gggacagccg ctaaccttct tttttcggga agtaaatgca ggaatttgga
    20821 acagaagaaa gacagaattt taaggagaat gaggtaagga atgaaagaaa tccagtatga
    20881 gattgtaaag gaaatcgccg tattgtctgc gagtgacagc ggctacacaa aggaaatcaa
    20941 tcttatttca tggaacggga gagagccgaa atatgacatc cgcagctttt ccccgaaccg
    21001 tgagaagtgc ggaaagggta tcacgttgaa cgctgatgaa gcagcggcac tccttgaagc
    21061 attacagaaa gaagtaaaca gcggggattg atggtatctg attgacaggg cggggcgttt
    21121 ccagacgttc tcctgccctg tctggaaagg aagatttaag tatgggcgag gataggaaaa
    21181 caactggaag aagaaagcgt attgtgccaa aagggcaagt acaaatgatt atcagtcgag
    21241 aatatatcgg cacacagacc gttgcagaag cgtttgtccc gattatctcc gaggatattc
    21301 ggaagaagat tgccgagggc gacaccttcg acaatgaggg gctgtccgct tagaatgtac
    21361 gcaatggaac atgaaaacgt taggacagat atggaggttt tacagtatgg caggaataag
    21421 aacggagaac aagatttatg aagtcggcat ttactgccgc ttatcaaagg acgatggcac
    21481 ggataacgag agtgcgagca ttgcgacaca aaaatccatc ctcacggatt atgtgaaaag
    21541 gcagggatgg catttagcaa aaacgtatgt tgacgatggc tactctggaa caaatttcca
    21601 aagaccaagt ttccagaata tgattaagga cattgaaaac gggctgataa actgcgtgat
    21661 tacaaaagat ttatcccgtc tggggaggaa ctatcttgat tgtgggttat atctggaagt
    21721 ctttttccca gagcataacg tgaggtatat agcggtcaat gacggcgtgg acaccttaaa
    21781 caaatccgct atggacatca cgcctttccg caacatccta aacgaaatgt attctgccga
    21841 tgtgtcagtc aagataaaat cggcgtaccg tgcgaggttt cagcagggga aattcatggg
    21901 aacaacagcc ccttatggct atgtcaaaga ccctgccgac cacaaccatc tgctgataga
    21961 tgacaaagtt gcccatgtgg taagggagat attcgacctt gcgttagcgg gcaacggcat
    22021 cgccaaaatc cgcaagcaca tcaacaaaca gcatatccta cgccctgccg cttatgcggt
    22081 ggagcaaggg gcaacaggct atgagcggta ctttgaggat aacgaggaaa accgttatat
    22141 ttggagcgag aacagcgtga ggggcatttt aagaagcccg atatatgcgg gcaaccttgc
    22201 aggctacaag cggattgcag ccaacatgaa aagcaagaaa cgcccctcta agctgcccga
    22261 agaatgggaa gtgataccag acacccatga ggggatagtc acgcaggagg aatttgacac
    22321 cgtacagcag cttatgacga gccgcaggcg ggagcagaac gcagggggat ttgagaatat
    22381 cttttcgggc gttatcaagt gtgcggactg cggctatgcg atgcgggcgg cgagtgccaa
    22441 caggaggaaa cgccccgaca tcatcgactg cgtacaatac acctgcaata attatggcag
    22501 atatggcaat gttatgtgta ccgcacacag cattgaagcg agggacttat tcaatgccgt
    22561 ccttgccgac atcaaccgct ttgcggatat ggcggtcaat gatgaaaagg cggtcagagc
    22621 catagaaagg cggctcacgg aaacagacca gagcagggcg aaatcattag agaaagaaaa
    22681 gaagaagctg aacaaacgcc ttgcggagct tgacaggctg ttttcctctc tctatgagga
    22741 caaggtaatg gagcgtatca cggagcggaa ttttgagatg atgtcgggga aataccagaa
    22801 agagcagctt gaaattgaag cacggctgaa agaggtaacg gaaacgctca atgacagcta
    22861 tgagaaatcg cagggcgtga gggactttct atccctaatc cgcaactatc aggggctgaa
    22921 agagttggac gcaaccatca taaacgcact catagacaag atacttgttt cggaacgtga
    22981 gaaacttgcg gacgggacag taaggcagga aatcaagatt tactataaat tcatcggctt
    23041 tgtcggtgaa ttacatatca cacccacaaa gcggtggacg gcgttaaagc ctaaaaattg
    23101 tacggtgtgc ggtgttgaat acgtccccag ctctggaata tcaaagtatt gtcccgcttg
    23161 tgctaagagg atacagaggg agaaatcaaa cgagagcaaa cgcaggagta gggagaggaa
    23221 ccgaagggca tgtattgaac tgtccgcaaa aaatgaccga ctgatttggt gcagcgcaat
    23281 ggtaggttaa ttcgtcaagg caatatgcac caagaggtag atatttacca ttatattacg
    23341 aaagggagct tcgataacta cctctggcaa actcaggaga ataaactcaa atatatcacg
    23401 caaatcatga cctctaaaga tcctgtacgg tcggcagaag atattgatga gcagaccatg
    23461 actgcttcag actttaaggc tttagcaact ggtaatcctt atctcaaact gaaaatggaa
    23521 ctagagaatg agttgacggt gttggacaat caaaagcgtg ctttcaatcg ttctaaagac
    23581 gagtatcgcc ataccatagc ttactgtaag cagaacctcc ctgtcttaga gaaacgcctc
    23641 agtcaatatg accgtgatat tgtccaatct ttggcaacta agtcacaaga ttttatcatg
    23701 cgatttgata atcagatgat ggataaccgt gcagaagctg gagactatct acgtaaactc
    23761 attacctata accgttctga aaccaaagaa gtaagaacgc ttgcaacctt tagagggttt
    23821 gagcttaaaa tggcaaccag aagtcctagt gagcccttac cagatatggt gtctttaacc
    23881 atttcaggta gcaaccagta ttctgtctct ttagacttga aatcagatgt gggaaccatt
    23941 caacgcatta ctaatgccat agaccatatc ttagacgacc aagaaaagac agaagaaatg
    24001 gccaataatc taaaagataa gctatcagtc gcaagggttg aagtagagaa ggtctttcca
    24061 aaagaagaag attatcagct ggttaaagcc aaatatgaca tccttgcccc cttggttgaa
    24121 caagaagcag agattgagga aatcgatgca gcactagcta aattcaatga aacgggccaa
    24181 ccgcaacagg accaacaact ttcgcttgat ttttaagaaa aaggctgaca aaaaataatg
    24241 aacgcggtaa aatgaagtca agaaatcacc aaaggagaat tgattatgac ccagacagta
    24301 gaagaattaa gatatcaact agaagaatgg ctagcacaag gttttacaag ccctgaagat
    24361 agagctaact atcaagcctt aaaggaacag tatgaggatg aaacccttga ttacagcttt
    24421 tcaaagcgag aaattattgg tcaattagaa gttatcatca cgactcgaga gaatgacttt
    24481 ccagacttag atgaagtgac taaaggagag tatctagacc tggttgaaca acttgataat
    24541 ctagacaagg gacaggcaga ttattatcgt aagcaattag cgtagaaaga ggtacagata
    24601 tgattgaaca aattattcaa agtcttttca ttatcgtagc aacagggctg attttgcttg
    24661 tcctttacca gattgcgaag atgcttggaa gcttatttgt cattggttta atcggatttt
    24721 tagctttcac agaagtctat ggaatttacc tcttttttac agaacgatac ctctatgtgg
    24781 aagatttagc caccaatggt attttgagct ttaccacttt ctatattatt tttaatcttt
    24841 tacttgtctt tggacttgtt agaaaagttg ttagaagtcg gatggcatga tgtaatggga
    24901 ttacctaagt taggtagtcc ttttctttat tgtgataact tttccaagtc catcttgtta
    24961 tcatggtgct atcttatgga aaaaggagat agcactagtg atgttaaata aaatgaaggc
    25021 tcggctattg attggtttag gaggtctagc tgtgactagc tttatggtga tgatgggtta
    25081 caccattggt tctcagtctg ttaccaatca cacgcagaaa caaatccaat ctgaggctca
    25141 aaaattactg accaaagaga aagaaaaaga gaaggcaagt gtcctctctg atgagttagt
    25201 caaggaattt ctcacccaat actatactaa ggaaaagctg ggcgagaata acaaccgtat
    25261 taaaccctat atgacagatt ctgcctttaa ggaagaagta gctcgtcaag aagattccat
    25321 caaccaagtc tataaagact atatgttgga ttatcgcttt gagtctgcca atatctatgt
    25381 caatgcagaa gaaaatgaag cttttgcgga agtgtcttat caggttgttt acgtgtcaga
    25441 tgtcagtgat aaggctcata aaagcacaca gacagaaaca agaactctta aactatctta
    25501 tgccaaattg tcagataaac tctttgtcaa tcaggtaacc atttggaatg ggaagctaga
    25561 ggacttaaaa gagtcttctg atgaagtaaa ttcaagcatt ccaaagattc aaggcactac
    25621 tacaagtgag accaactagc aggagttact cttctgcttt ttttgataag gagatggcat
    25681 gacaagaatt gaaaaagtga aacaaaaggc gattttagag gtggcagaaa gtctaggtta
    25741 ttcttttaaa cgactatcag ggcaagtcta tgaacatcca gaacacgatt cctttaggat
    25801 ttttgcggac accaatacct ttaaatggtt ttcacgtgac gtacagggag atgtgattga
    25861 ttttgttcag ttaatgacag gagtgtcctt taaaaaagcc ctgtcttacc ttgaaactgg
    25921 agactttgaa caagctaaaa tcgtcgaaga gacttaccaa ccctttcgtt attatctaag
    25981 agaagaacca tttgaccaag cacgaactta cttaaatgat attcgtggtt taagtgatga
    26041 cactatcaat gcttttggca gacaaggtct attagcacag gctcattacc agacaaagac
    26101 ctttcaggaa tcagttcttg tctttaagag ttacaatcat cttggccaac tagagggggc
    26161 aagtcttcaa gggcttgtca aagataatca gagacatgat cgaggttacc tcaagaaaat
    26221 catgaaagac tctcatggct atatagggat gagctttgat attggaaaac cgaaacgcct
    26281 tatcttttgt gaatcggtga ttgatatgat gagttattat caactccatc agaagcagtt
    26341 atcagatgtt cgtttggttt ctatggaagg cttaaaactc tctgtgattg cgtatcagac
    26401 tttacgatta gcagctgagg aacaaggcaa acttgagttt ttagatactg tcaaaccaag
    26461 tcgattaacg cattaccttc atgctataca agagacgact accttctttc agacgcaccc
    26521 aggcttatta accttggctg ttgacaatga tgaagcaggg agagattttt gtcagaaatt
    26581 atctgagaaa ggacttccca ctgaaacaga ctgtccacca ttacaggaac tggaaactaa
    26641 agcagattgg aatgatattg taaaatatca aaataattat tctctaaaag atgtgatcca
    26701 atcggccaag ttacaagtta tccgaagcaa tcctccgcct agaaaaaata ccgctttaga
    26761 gttgtgataa caaaatcaag tttcttagct taatctagaa agtgataagg aggacaccct
    26821 atgagtgtga ttgaacgcct agctgaaaga gtggctaggc aagaggaaaa agttgcgaaa
    26881 gaaacggaaa aactagaaac ttatcgcagt caattacaaa cagcgatgta tcaaaccttt
    26941 atcaaacgcc aacaaaacag tccttggaca tttgatgaag cattgacgca agcctttggc
    27001 caagatgaac aagaactaaa accatcagac actagaaatg aggaataaca catgacaaaa
    27061 gattggacct ttgaccaacc cttagatgat aacacaccga catcttcatc ggaagaacgt
    27121 gcgaagattg cagcactatt tcataaagaa aaccaagagg gcactgaaga tgttgactat
    27181 gtggcagcct ttgaaatgga acaacaaaaa gctaaggaga aatccgtttc acaaccaaaa
    27241 actcaagagc aatccacacc aaaagaggtg tcctcttcta acattacaaa cgactacaag
    27301 caacatctag ctgatgtgat ggctcagaat aatgaagaca ttcttcaaag tcagaagaaa
    27361 atcgaagagt tgcaccagtt gattgatgac aaaaataagc aaaataagaa attacaagcc
    27421 atttcagcag ctattgatga cttgtaagtg aggcagtcct tacaagggct gctttttata
    27481 aggaaataag atgaagttat ttaaaaagca aacgcataat caagatagtt ttaagcgtct
    27541 tattcatcgt ttatcaggaa tgtctgaagc cgacttacat aaggttaacc aattactaga
    27601 tgtcgttttt gattcaacca gccaaacgac caatgacgta aaacctccta aagatttaca
    27661 aaaggagcct accctagatg agactataac tgaagctaag aataaattga agactgagca
    27721 actcgaaaaa cggatagagc aatttaggca ggcgaagaaa ccaaaaagcg aataac
//
LOCUS       DBNFEG010000008        27081 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.008, whole genome shotgun sequence.
ACCESSION   DBNFEG010000008 DBNFEG010000000
VERSION     DBNFEG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 27081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27081
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.008"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            5..1486
                     /locus_tag="AC1PNG_000216"
     CDS             5..1486
                     /locus_tag="AC1PNG_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HIP6700755.1"
                     /translation="MSRFPKNFLWGGAIAANQAEGAYNVDGRGLVQTDVTTGGSVNSP
                     RYTTYIDKDGKPGKYASMGHAGGHLPEGATYAVLEDHYYPNHKAVDFYHRYKEDIKLF
                     AEMGYSVFRLSISWARIFPNGDDAEPNQAGLDFYRSVFEECRKYGIEPLVSIWHFDTP
                     LSLEQRYGGWTNRKLIDFYVRYAETIFNEYKGLVKYWITFNEINNTIMFLDMFSEPED
                     SDYQEAYQHLHHQFVASAKAVKLGHQIDPSYRFGNMICGITFYPGTCDPADILENQHK
                     WEQNIFYCSDAQAKGRYGSYANRLWKEHNVVLQMEEGDLEILAEGKVDMYTFSYYMST
                     VVTTHKVEDTVSGNFSIGAKNPYLTYSDWGWAQDPTGLQYYLEKIYDRYEIPMMIVEN
                     GLGALDTVEEDGSIHDDYRIDYHRAHVNAMADAIANGVDLIGYTTWGCIDLVSAGTGE
                     MRKRYGFIYVDMDDAGNGTLARTPKDSFYWYKKVIASNGEDVE"
     gene            1724..2107
                     /locus_tag="AC1PNG_000217"
     CDS             1724..2107
                     /locus_tag="AC1PNG_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002962841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIP6700756.1"
                     /translation="MKIEHVAIYTQDLEGMRNFFENYFNATSNQLYHNLKTSFKSYFL
                     TFEDGVRLEIMTRDDVVDKPSQLNYLGLIHLAFSLGSEEAVDELTERLVAAGYLLLSG
                     PRITGDGYYESCVLGFDDIQIELTV"
     gene            2403..3836
                     /locus_tag="AC1PNG_000218"
     CDS             2403..3836
                     /locus_tag="AC1PNG_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HIP6700757.1"
                     /translation="MSKLPKNFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVP
                     REITAGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEAGLQFYDNLFDECLKYGIEPVVTLSHFELPYHLVTEYGGFRNRKTIEFFVRFAETC
                     FRRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYQEGENREAVMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPATCKPSDILMAQKAMQKRYYFADVHVHGFYP
                     EHILRYWERKGISVDFTEEDREDLLEGTVDYIGFSYYMSFAIDSHRENNPHYDYLETE
                     DLVKNTYVKASDWEWQIDPEGLRYALNWFTDMYHLPLFIVENGFGAIDQVEEDGMVHD
                     DYRIDYLGAHIKEFIKAVDEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDE
                     GNGSYARSPKRSFAWYKNVITTNGEEV"
     gene            3917..4156
                     /locus_tag="AC1PNG_000219"
     CDS             3917..4156
                     /locus_tag="AC1PNG_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009568309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HIP6700758.1"
                     /translation="MVGFIAACLTTFGFVPQVIKVVKTKDTESISLGMYMMSVAGMSL
                     WLFHGIIQGDTALIIANSLSVTLAGIILVYKLVYK"
     gene            4380..4718
                     /locus_tag="AC1PNG_000220"
     CDS             4380..4718
                     /locus_tag="AC1PNG_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700759.1"
                     /translation="MESIVGIVVIVVVTVVLVYLLTNMWKIRAVSVGQTDDTTLYFVR
                     FKGVNPPDLANSSVFNQVAKIKCAKGDTYQVTSTVNDVKLRDVIMDEFQLKSSEVVVY
                     YPPRLFFYAS"
     gene            4896..5210
                     /locus_tag="AC1PNG_000221"
     CDS             4896..5210
                     /locus_tag="AC1PNG_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HIP6700760.1"
                     /translation="MVKALIICAGGMSSSLIAKKTQTLLEEQGHKVEMNAVGVPEGGK
                     KINAAEYDLYLISPQTKMHFKQFEEAGKKVGKPIVQIPPQAYIPIPMGIEKMAKLVLD
                     NI"
     gene            5257..7230
                     /locus_tag="AC1PNG_000222"
     CDS             5257..7230
                     /locus_tag="AC1PNG_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIP6700761.1"
                     /translation="MLSTKEKMILQYLYQHQNVFSTSKVLAEHLSYTDRTIRTYIKKM
                     ASEISERETGFAILSKQGYGYQLRISDEEKYHRFLSENQLVFGVDYSDAENRHKQILN
                     KLIFEEECILFDDLADQLFVSRSTLSHDFKHIREHLASYGLSIESKPYKGVYVVGEER
                     QKRHFIMDYFFSEHFYQNIHHFLSNDKSLALPLSFEELTIVVLDECRDSQLKLSDYII
                     QNLVIHLGLAIQRYQKGFTISPVTIDKEKYAEELAVATKIARRLSHRLEPQNRFPEEE
                     VVYIALHLISKTQLEVEKSSDRLLLRQALFEVLGRYDDEYGYQFSHDFSLIEGLLTHL
                     EVLVERLRHNLHIDNPLLDDIKTTYHDIFDLTQSLMAELSVFHSYSLSESEIAYVALH
                     LMASLERQKEKYKLNVLVICATGYGSAQMLKNRIKNELGQQVSISDVIGYYDLDNQKL
                     QGVDVIMSTIDLSGLVFKVPVITVSVFLTDQEVKEVKQRLADLRPSLQKTQVVERESN
                     LEKYFDQYFSEQRFLVVESAEKEMLLKQMISQLESEGLGNHQEFYKLIEARESLSTLV
                     FDKDIAVPHPLKPISEKHMISVAVIKNGLYWEEGYGQIRLVFLVSPSIYTNEGLSTIT
                     NRLVDLVDLPEVKKDIINSPDFETFKHVFLQRE"
     gene            7241..7555
                     /locus_tag="AC1PNG_000223"
     CDS             7241..7555
                     /locus_tag="AC1PNG_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HIP6700762.1"
                     /translation="MNTEELQMAAFGIILSSGNARSVIHEAFAAMREGDYDKAEELLE
                     AANADMLEAHHSQTGLLQEYASGTEVKVEIIMVHAQDHLMTTMTLREVALEMLALYRK
                     VG"
     gene            7570..8082
                     /locus_tag="AC1PNG_000224"
     CDS             7570..8082
                     /locus_tag="AC1PNG_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700763.1"
                     /translation="MSKGMASKKLQKTPEQLKQESKRKSQLFNRYMLFRYSLAVFFFA
                     NLYWLLVQLFQPSLYLILPSLMLIAIIIATAEQFKLYSAEESRLTKTEFVLRAQAILQ
                     GLIVVLVILGHISTLFPTFSNHTSAKLFVIGLQLLGIALVVLNLKRLEQVKQNTDKYY
                     LRFQIIRKYL"
     gene            8102..9451
                     /gene="celB"
                     /locus_tag="AC1PNG_000225"
     CDS             8102..9451
                     /gene="celB"
                     /locus_tag="AC1PNG_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HIP6700764.1"
                     /translation="MEEQKGFFGLLDKYLMGPMGKLASYKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVSDIFAASFDKFTPLYMVANTATMGVLSLYFALAIGYEL
                     TKIYAEEEELNLTPLNGALLSLFAFIMTVPQIIFEEGAMSAVSSLEEGAVIVDGWAMG
                     GGVTRFGTTGIFTAIIMAVAAVLLYRMCVKNNWVIKMPDSVPEGVSRGFTALIPSFVV
                     AFFVIFLNGVLVALGTDIFKVIAIPFSFVGNLTNTWIGLVIIYLLTQALWVVGIHGAN
                     IIFAFVSPIALANMSLNAAGERMIVAGEFSNMFVIAGGSGATLGLCIWLATRARSEQL
                     SAIGKAAIVPGIFNINEPLIFGLPIIYNPALAIPFMLAPIASMTVYYFSMKLNLINAV
                     VAQVPWPTPVGIGAFLGTADWRAIVVSIVCAVVAFLVYYPFIRAYDKQLLKEEAANA"
     gene            9857..11323
                     /locus_tag="AC1PNG_000226"
     CDS             9857..11323
                     /locus_tag="AC1PNG_000226"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIP6700765.1"
                     /translation="MTKQFPKGFLWGGATAANQYEGGWNLGGRGPATSDTYIAVDPDK
                     RKDMSHFGKPVSRADVEFALADQEGLYPKRWGSDFYHRYKEDIALFAEMGFKTFRLSI
                     AWSRIFPKGDELEPNEEGLAFYDAVFDELNKYGIEPLVTLSHYEFPLHLALEYGGWKN
                     RKVIEFFVRYAETVFKRYQGKVKYWLTFNEINILGMVGYLSGGLLFEDGKNDLEAMYQ
                     AVHHQFIASSLATKAAHEIDPENKVGCMLARMENYAATCNPDDVLAALKKDQENLFYT
                     DVQVRGAYPSYMKRFFKENNIQVVFEPGDEAILKQYPVDFMSFSYYMTSITRALPDKD
                     KATAGNLILGEANPYLEASDWGWQIDPVGLRITLNKLYDRYQVPLFIVENGLGALDKV
                     EADGSIEDGYRIAYLEKHIQQMYEAIEDGVELMGYTPWGCIDLVSASTSEMSKRYGFI
                     YVDADDQGKGSFDRSRKASFYWYKDVIASNGANVLTEK"
     gene            11332..11934
                     /locus_tag="AC1PNG_000227"
     CDS             11332..11934
                     /locus_tag="AC1PNG_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIP6700766.1"
                     /translation="MTKTIYLMRHGETLFNTQKRVQGWSDSPLTERGIAQAQAVGQYF
                     KEQGIVFTSAYSSTQERATDTLKLVTDAPYQQLKGLKEMNFGIFEAREEELLPKHRPG
                     ARSFEDLLVPFGGEDIRAVGSRVKEAILSVAESDPADPILMVSHGAAIWGLVQVLEVT
                     FPPGVFLPNCAICQLTVENEQIRFEKLIDSAHDFKVYDFK"
     gene            11946..12563
                     /locus_tag="AC1PNG_000228"
     CDS             11946..12563
                     /locus_tag="AC1PNG_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIP6700767.1"
                     /translation="MNYLYLMRHGQTRFNQQGRIQGACDSPLTELGREQALAAHQYFQ
                     EQGIEFDKIYSSTQERACDTAELVTGRTDYIRLKGLKEQDFGAFEGQQEYLNPPLQGD
                     IGYGDYFVTFGGESYQDVRQRMVETIGGIMEEADNQSVLVVSHGAAIAQFFRQVLTDY
                     PQVRMRNCAILTFTYEDGHYDLQSIVDPVNKENLYQKEGKEKEKK"
     gene            12560..13999
                     /locus_tag="AC1PNG_000229"
     CDS             12560..13999
                     /locus_tag="AC1PNG_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HIP6700768.1"
                     /translation="MTVTKVFPKGFLWGGATAANQCEGAYDVDGRGLANVDVVPIGQD
                     RAAIITGRKKMFDFEDGYFYPAKESIDMYHHFKEDIALFGEMGFKTYRLSIAWSRIFP
                     KGDELEPNEKGLQFYEDLFKECHKYGIEPLVTITHFDCPMHLIENYGGWRNRKMLEFY
                     ERLCRTLFTRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENVEQVKYQAAHHELV
                     ASAIATKLAHEIDPENKVGCMLAAGQYYPHTCHPRDVWEALQEDRENYFFIDVQARGY
                     YPNYAKKKWERAGIEIEMTNQDLALLKEHTVDFISFSYYSSRVASGDPVEKEKTAGNI
                     FASIKNPYLDASEWGWQIDPLGFRITINSIWDRYQKPLFVVENGLGAVDTPDENGYVE
                     DDYRIDYLRQHVLAMRDAIVEDGVELMGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDN
                     EGNGTLKRSKKKSFEWYKKVIATNGADVG"
     gene            14083..15072
                     /locus_tag="AC1PNG_000230"
     CDS             14083..15072
                     /locus_tag="AC1PNG_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HIP6700769.1"
                     /translation="MKYIVNNSNDPAYNIALEAYAFKELTDIDEIFILWINEPAIIIG
                     KHQNAIEEINKEFTDEKGIHVVRRLSGGGAVYHDLNNLNYTIISNKADEGAFDFKTFS
                     KPVIDTLATLGVEANFTGRNDLEIDGKKICGNAQAYAKGRMMHHGCLLFDVDMSVLAS
                     ALKVSKDKIESKGVKSVRARVTNINNELPEKMTVLEFKDAILNQMKQEYPDMDEYVLS
                     EGDLVRIQEIRDTQFATWDWTYGQTPEYTVERSVRYPAGKITTYIKAEKSVIESIKIY
                     GDFFGIGDVSDIEDLLVGTRYEYADVLAKLQEIDTTHYFSRMTTEEVAKAIVA"
     gene            15244..15534
                     /gene="rpsF"
                     /locus_tag="AC1PNG_000231"
     CDS             15244..15534
                     /gene="rpsF"
                     /locus_tag="AC1PNG_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIP6700770.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATIVESKAWE
                     KRRLAYEIKDFREGLYHIVNVEANNDEALKEFDRLSKINGDILRHMIVKLDA"
     gene            15546..16040
                     /locus_tag="AC1PNG_000232"
     CDS             15546..16040
                     /locus_tag="AC1PNG_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIP6700771.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNQAVATFTLAVNRNFKNQNGER
                     EADFINVVIWRQQAENLANWAKKGALIGVTGRIQTRSYDNQQGQRVYVTEVVAESFQL
                     LESRTAREGQGGGYSAGNSFAGGNDYNSPYQAPAQSTPNFAREESPFGASNPMDISDD
                     DLPF"
     gene            16073..16312
                     /gene="rpsR"
                     /locus_tag="AC1PNG_000233"
     CDS             16073..16312
                     /gene="rpsR"
                     /locus_tag="AC1PNG_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIP6700772.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFISERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALLPFVNED"
     gene            complement(16703..17359)
                     /locus_tag="AC1PNG_000234"
     CDS             complement(16703..17359)
                     /locus_tag="AC1PNG_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HIP6700773.1"
                     /translation="MSFTEINGLTKKNQEFIHIATNQLIKDGKSDSEIKELLEEILPT
                     IIEKQKTGVTARNLYGAPSEWAASKTVSEQEKKDQVEYNENPWLMWLDSSLFMLAIIA
                     GINGLMNLFGQGAQYGLLTLFVIGFGVGAGMYLMYHFVYREQIKTGQRPKLLKAIAFL
                     GLATLAWSVVFILAALIPAAFNPVLPPLVTILIGAAAFGARYLLKKKYNIRNAMSPVQ
                     "
     gene            complement(17375..18319)
                     /locus_tag="AC1PNG_000235"
     CDS             complement(17375..18319)
                     /locus_tag="AC1PNG_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIP6700774.1"
                     /translation="MKQIFLSTMTDLEEIQTFEPGAWINLVNPSQSESMEVAEHYKID
                     IADLRAPLDAEESSRITVEDDYTLILVDVPILEERNNKTYYITIPLGIILTDDAIITT
                     CLEPLPLFEQFIHRRLRNFFTFMKSRFVFQILYRNAQLYLSALRTIDRKSEEIEKQLH
                     EATRNEELIVLMELEKTIVYFKASLKTNERLVKKLASSSRLLRKYEEDEDLLEDTLVE
                     TQQAIEMADIYGSILNSMTHAFASIISNNQNTIMKTLALVTIVLSIPTMVFSAYGMNF
                     KDNIIPFNDIPYAFWWIILFAFAISLSLTFYFIRKRWF"
     gene            19158..21983
                     /gene="uvrA"
                     /locus_tag="AC1PNG_000236"
     CDS             19158..21983
                     /gene="uvrA"
                     /locus_tag="AC1PNG_000236"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIP6700775.1"
                     /translation="MQENIVIHGARAHNLKNIDVTIPREKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMDKPDVDSIEGLSPAISIDQKTTSRNPRSTVGTA
                     TEINDYLRLLYARVGVPYCINGHGAIAASSVEQIVDEVLELPERQRLQILAPIVRKKK
                     GQHKTIFEKVQKDGYVRVRVNGDVYDVSEVPELSKSKAHNIEVVVDRIVIKEGIRSRL
                     FDSIEAALRIADGYVIIDTMDDKELLFSEYYACPVCGFTVPELEPRLFSFNAPFGSCS
                     DCDGLGMKLEVDTDLIVPDASKTLRDGALAPWNPISSNYYPQMLEQAMNHFGVDMDKP
                     FEELTEEEKNLIFNGSDGKEFHFHYENEFGGVRDIDIPFEGLITNINRRYRETNSDYT
                     RTVMKTYMNELTCGTCHGYRLNDQALSVKVGGEQGLHIGQLSDLSVADHLEVIEHLTL
                     SENEATIATPIVKEIKDRLSFLNNVGLNYLTLSRAAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADWLIDIGPGA
                     GVFGGEIVASGTPAQVAKNKKSITGQYLSGKREIPVPLERRVGNGRFLEVTGAKENNL
                     QDVTVRFPLGKFVAVTGVSGSGKSTLVNSILKKAIAQKLNRNSDKPGKFKSISGIEHL
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVFVPCEVCHGHRYNSETLEVHYKEKNIAQVLDMTVNDAVE
                     FFKHIPKIERKLRTIQDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHTEDIAQLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDMGPEGGVGGGT
                     VVATGTPEEVASNPASFTGQYLKMKLK"
     gene            22082..23143
                     /locus_tag="AC1PNG_000237"
     CDS             22082..23143
                     /locus_tag="AC1PNG_000237"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIP6700776.1"
                     /translation="MQARVEKFEAKLAQSDVDGILVTGQNNIYYLTGFWGTEATVFIS
                     GKRRLFVTDSRYTLIAKASVKGFDIIESRFALEEIAKVIKEDGLEKIGFDSEVTYGFY
                     QSLTSIFEGYQLVAMSNFIEDLRMIKDEKEIATIRRACQISDQAFIDVLDFIKPGQTT
                     EMDVNHFLDHRMRQLGAEGASFEFIVASGYRSAMPHGRASEKVIQAGETLTLDFGCYY
                     QHYVSDMTRTIHIGHVTDQEREIYDVVLRANKALIEQAKEGVTYREFDAIPREIINAA
                     GYGANFTHGIGHGIGLDIHEYPYFGKSDETIKAGMVLTDEPGIYLDDKYGVRIEDDLL
                     ITENGCEVLTLAPKELIVI"
     gene            23207..23764
                     /gene="efp"
                     /locus_tag="AC1PNG_000238"
     CDS             23207..23764
                     /gene="efp"
                     /locus_tag="AC1PNG_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIP6700777.1"
                     /translation="MIEASKLRAGMTFEAEGKLIKVLDASHHKPGKGNTIMRMKLRDV
                     RTGSTFDTTYRPDEKFEQAIIETVPAQYLYQMDDTAYFMNTETYDQYEIPVATIKEEL
                     LYILENSDVKIQFYGTEVIGVTVPTTVELVVAETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIEAGQKLVINTAEGTYVSRA"
     gene            23792..24184
                     /locus_tag="AC1PNG_000239"
     CDS             23792..24184
                     /locus_tag="AC1PNG_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIP6700778.1"
                     /translation="MTTEYQGEIVIAPRVLEVITGIAAAKVDGVHSLQNKRVADSLSK
                     TSLNKGVYLETDEEGRVTADIYVYLEYGVNVPAVSMDIQRAVKTAVYNYAEVQVAAVN
                     IHVSGIVPDKTPKPALKDLFGEDFLDEE"
     gene            24174..24593
                     /gene="nusB"
                     /locus_tag="AC1PNG_000240"
     CDS             24174..24593
                     /gene="nusB"
                     /locus_tag="AC1PNG_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIP6700779.1"
                     /translation="MKNNRHALRKCALQAIVSLEFGQEPVQAAQFSYLYDREEEQEGV
                     EIPLFLLNLVNGVADYRDDLDKELSSRLKAGWTLDRLTLIDKNIMRLGLFEILHFEET
                     PDRVAVNEAIELAKEFSDESSAKFVNGVLSQFIKEEA"
     gene            complement(24672..25637)
                     /locus_tag="AC1PNG_000241"
     CDS             complement(24672..25637)
                     /locus_tag="AC1PNG_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIP6700780.1"
                     /translation="MVAKLTDVAQLAGVSPTTVSRVINKKGYLSEKTIRNVEEAMREL
                     GYKPNNLARSLQGKSAKLVGLIFPTINNIFYSELISHLEKELFDRGYKTIICNSQHES
                     DKEREYLEMLAANQVDGIISGSHNLGIQDYDRVVAPIIAFDRNLSPSIPIVSSDNFAG
                     GVLAAQTLQKAGCHNPLMITGNDDSNSPTGLRQVGFTSILNKAEVFHISSDFSPVRKE
                     MEIRAILEKQKPDGIFVSGDATAMLVWNVIRSMKLSIPEDIKLIGYDGTSFIENYYPQ
                     LTTIKQPLTEIARLLVELLVDKIEGKKLTKTDYILPISLLAGASI"
     gene            complement(25618..27072)
                     /locus_tag="AC1PNG_000242"
     CDS             complement(25618..27072)
                     /locus_tag="AC1PNG_000242"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HIP6700781.1"
                     /translation="MAFTTEERYRAYADWTPEYIEKIKKNTENSPWRTNFHIETPHGL
                     LNDPNGFSYFNGKWTLFYQYFPFGAAHGLKSWVHTESTDLVHFEKTGTVMYPDTPLDS
                     HGAYSGSAMEFGDKLFLFYTGNVRDENWVRHPYQIGALMDKDGKIEKIDKVLIEQPED
                     TTDHFRDPQIFNYKGQYYAIVGGQNLDKKGIVKLYKAENNDYLNWSYVGDLDFANDMT
                     AYMMECPNIAFVGEQPILLYCPQGLDKEVLDYSNIYPNMYKIGQSFDPETASIVEPSE
                     LINLDYGFECYATQAFNAPDGRTLSVSWLALPDVEYPTDAYDYQGCLSLVKELTIKDG
                     KLYQYPVDAITSLRKEAQPFSAQKETNNVYELELDFSAGTKHEIVLFANEAEKGLVLS
                     VDTEQGQITLDRGNCGQPFALDFGTSRSCEISPGALTANIFIDKSVFEIFINKGEKVF
                     SGRVFPDADQSGIVITTGNPTGTYYHLDYGRKTN"
BASE COUNT         7829 a         5170 c         6164 g         7918 t
ORIGIN      
        1 aaacatgtca cgttttccaa aaaatttcct ctggggagga gctatcgcag ccaaccaggc
       61 agaaggtgcc tataatgtgg acggacgcgg tctagtccag acagacgtta caacaggagg
      121 atctgttaac agcccccgct atacgactta cattgataag gacggcaagc caggtaaata
      181 tgcttctatg gggcatgctg gcggtcatct gccagaaggg gcgacctatg ccgtcctaga
      241 agaccactat tatccaaacc acaaggctgt ggacttctac caccgctata aagaagatat
      301 caagctcttc gcagaaatgg gttactccgt tttccgcttg tcaatttcat gggcacggat
      361 tttcccaaac ggtgatgatg cagagccaaa ccaagcaggt cttgatttct atcgttctgt
      421 ttttgaagaa tgccgcaaat atggcattga gccattggtt tccatttggc acttcgatac
      481 acctttgagc ttggaacaac gctatggtgg ttggaccaat cgtaagttga ttgatttcta
      541 tgtccgctat gctgagacaa tttttaatga atataagggg ttggtgaaat attggattac
      601 cttcaatgag attaacaaca ctatcatgtt tttggatatg tttagtgaac cagaggattc
      661 tgattaccaa gaagcctacc aacacttaca tcaccagttt gttgcctctg ccaaggctgt
      721 aaaattaggt catcaaattg atccaagtta tcgattcggc aacatgattt gtggaatcac
      781 cttctaccca ggaacctgtg accctgcgga tattttagaa aatcaacaca aatgggaaca
      841 gaatattttc tattgtagtg atgcacaagc caaaggtcgc tatggttcct atgcaaacag
      901 actgtggaag gaacacaatg ttgtattgca aatggaagag ggagacctag aaatccttgc
      961 tgaaggcaag gtggatatgt ataccttctc ttattacatg tcaacagttg tgaccactca
     1021 taaagtggaa gacacggttt ctggaaactt ctcaatcggt gccaagaacc cttacttgac
     1081 ctattcagac tggggttggg ctcaggatcc gaccggtctt caatactatc ttgagaaaat
     1141 ctacgaccgc tatgaaattc cgatgatgat tgtagaaaat ggtttgggag ctttggatac
     1201 agttgaagaa gacggctcta ttcacgatga ttaccgtatc gactaccatc gcgcccatgt
     1261 caatgctatg gcagatgcta ttgccaatgg tgttgatttg attggctata cgacttgggg
     1321 ctgtattgac ctagtatcag caggaacagg tgaaatgcga aaacgctacg gctttatcta
     1381 cgttgatatg gatgatgctg gaaatgggac acttgctaga acaccaaaag attcattcta
     1441 ctggtacaag aaagtcattg ccagcaatgg tgaggatgta gaataggaaa acggcaatgt
     1501 gccgtttcag attgaagaag aagtccattt tggacaattt tcttcaatct ttttttcttt
     1561 atgatgaaaa ttaaaaactc tcagaaacgc cgaaatatca atgttcctaa gagtttaatg
     1621 acttgttatc tttcacaaac ttcttcaatt ttttatattt ttaggagttt gtctaggttc
     1681 tgagacggca ctttgctgtt ttctcttagg gaaaggtttg aaaatgaaaa tagaacatgt
     1741 agcaatctac actcaggatt tggaaggtat gcgtaatttc tttgaaaact acttcaatgc
     1801 cacatccaat caactctacc ataatctaaa aaccagtttc aagtcctatt ttcttacctt
     1861 tgaagacggg gttcgtttgg aaattatgac cagagacgat gtggtcgaca aaccaagtca
     1921 actcaattat ctaggactta ttcatttagc ctttagtcta gggagtgagg aagctgtgga
     1981 tgagttaaca gagcgacttg ttgctgcagg ctacttgctt cttagtggac cacgaataac
     2041 tggggacggc tactatgaat cgtgtgtgtt gggatttgat gatatacaaa ttgaattaac
     2101 agtgtaatgt tggcaagtaa tactgctaga tcaatgttta acggattagc ttgcaagtat
     2161 cattcattag cgatttcctt actgttacgg aaaaaactgc aatggtgatt gatgtaaacg
     2221 tttgctaaac taataactat aggaacctgt attcacaagt agggtgcttg tatataagag
     2281 atgtttttga gggaatactg actgtatttt gaaaaaaaac taacaatgag tagcttaaaa
     2341 attagtctta gatggaaatt ctgaactgta aatacaggtt ctaaaatata caggaggcaa
     2401 ttatgtctaa attacctaaa aattttttat ggggtggagc agtagcagct caccaacttg
     2461 aaggtggctg gcaagaaggt ggaaaaggga tttctgtagc agatgtgatg actgctggga
     2521 gacacggggt acctagagaa attacagctg gcgtattaga aggaaaatat tatccgaatc
     2581 atgaagcgat tgatttctat caccgctata aagaagatat tgcactcttt gctgagatgg
     2641 gcttcaagtg tttccgtact tcaattgcat ggacacgtat ttttccaaag ggcgacgaac
     2701 tggaaccaaa tgaagctggc ttgcagtttt atgataactt atttgatgag tgtttaaaat
     2761 atgggattga acctgttgtt accctatcac actttgaatt gccttaccat ctggtaactg
     2821 agtatggtgg ttttagaaat cgtaaaacca ttgagttctt tgttcgtttt gcagagacct
     2881 gcttccgtcg ttacaaagat aaggtcaaat actggatgac ctttaacgag attaataacc
     2941 aagcaaatta tcaggaagat tttgcacctt ttaccaactc aggtatcgtt tatcaagaag
     3001 gtgaaaatcg tgaagcagtt atgtatcaag cggcccatta tgaattagtt gcctctgcac
     3061 gcgctgttaa aattggacat gaaattaatc ctgactttca aattggttgt atgattgcca
     3121 tgtgtccgat atatcctgca acctgtaaac catcagatat tttgatggca cagaaggcga
     3181 tgcaaaaacg gtattacttt gcagatgttc atgtgcatgg tttctaccct gagcatatct
     3241 tgcgctactg ggagagaaaa ggtattagcg ttgatttcac agaggaagat cgcgaagatt
     3301 tgctagaagg aacagttgat tacatcggtt tctcatacta tatgagtttt gctattgatt
     3361 ctcaccgcga gaataatcct cattacgatt atcttgagac agaggacttg gtgaaaaata
     3421 cctatgtgaa ggcttctgat tgggaatggc aaattgaccc agaaggattg cgctatgcat
     3481 taaactggtt tacagatatg tatcatctgc cactttttat cgtagagaat ggctttggtg
     3541 cgattgacca agtcgaagaa gatggaatgg tgcatgatga ctaccgtatt gattatcttg
     3601 gtgctcatat caaagaattt atcaaggctg tcgatgagga tggagttgac ttgatgggat
     3661 atacgccgtg gggttgtatt gacctggtgt cagcagggac aggtgaaatg cgcaaacgct
     3721 acggttttat ctatgttgat aaagatgatg aaggaaatgg tagttatgcg cgttctccaa
     3781 aacgctcatt cgcatggtac aaaaatgtca tcacaacaaa tggtgaggaa gtttaatgag
     3841 aagacctctg tagcgtaata gctacatggg tcttcattta tgtttttagt attggtatat
     3901 taaaggagaa aattgtatgg ttggttttat cgcagcttgc ttaactacct ttggatttgt
     3961 acctcaggtt atcaaggtag ttaagacaaa ggatacagag tcgatttcgt taggaatgta
     4021 tatgatgtct gtggcaggga tgtccttatg gttgttccat ggtattattc aaggagatac
     4081 ggccctcatt attgctaata gcctatctgt aacattagca ggtattattt tggtctataa
     4141 attagtctac aaataatgca ctattccatc gataaaagaa tttttagcaa cagtgaaaaa
     4201 cattgaaaat gcttgacttt ttctgtgaaa ggaatataat gatgacaaat aaatattgga
     4261 agtgtgatta ctatgttatt cgttccgctt tttttaaggt ttaacctttt taaaccacca
     4321 agataagcgt tttttctagt tgtttgtaag tgtatcgtat cttggaaaag aggtttatta
     4381 tggaatctat agttggaata gttgtcattg tagttgttac ggttgttctg gtttatttat
     4441 taactaatat gtggaaaatc agagcggttt cagttggtca aacggatgac actacgctat
     4501 actttgtaag atttaagggg gtaaatccgc cagacttagc aaatagtagt gtttttaatc
     4561 aagtagccaa aatcaaatgt gctaaaggag atacctatca agtcacttca acagtcaatg
     4621 atgtgaaact gagagacgtg attatggatg aatttcagct caaatcttct gaggttgtag
     4681 tttactatcc tcctcgtctt ttcttttatg catcatagtc gcaactcttc ggagttgcta
     4741 tttttttgtt tctggagagt agcggaaaag cggtttattt tagtagcgaa atttccgtag
     4801 tggtaaggaa atacttccta tggaaaatgt aagcgtgttc atgtaaaatg aaatcataac
     4861 aaaagtaaat aataaaatag ttggaggaaa atcctatggt aaaagctttg attatctgtg
     4921 caggaggcat gtcttcttca cttattgcta aaaaaacaca aacacttttg gaagaacaag
     4981 gtcataaagt agaaatgaac gctgttggtg ttccagaagg tggtaaaaaa attaatgcgg
     5041 cagagtatga cttgtactta attagcccgc agacaaaaat gcactttaag caatttgaag
     5101 aagcaggtaa gaaggttggg aagccaattg tccaaatccc accacaagct tatatcccaa
     5161 ttccaatggg aattgaaaaa atggctaaat tggtcttgga taatatctag ttctttgtta
     5221 ccatagatgg tatattgatt cgaaaagagg atagttatgc taagtactaa ggaaaaaatg
     5281 atattgcagt atctttacca acaccagaat gttttttcaa ccagtaaagt attggcagag
     5341 catctctctt atacagatcg cacaattcga acttatataa aaaaaatggc atccgaaata
     5401 tctgagagag aaacaggttt tgcgatttta tctaaacaag gttatggcta tcagcttcga
     5461 attagcgatg aggaaaaata ccatcgtttc ttatctgaaa accaactagt atttggggtg
     5521 gattatagtg atgcagaaaa tcgccataaa caaatcctga ataaattgat atttgaagaa
     5581 gaatgtatct tatttgatga tttggcggac cagttgtttg tcagtagatc aaccttgtct
     5641 catgatttca agcacattag ggagcatctt gcttcttatg gtttgagcat cgagagtaag
     5701 ccctataagg gagtctatgt agttggtgaa gaacgtcaaa aacgtcactt catcatggac
     5761 tatttcttta gtgagcattt ttatcagaat atccatcatt ttttatccaa tgataaaagt
     5821 ctagccttac ctctatcttt tgaagagttg acaattgtag tcttagatga gtgtcgtgat
     5881 agtcagttga aactatctga ttatattatc caaaatttag ttattcattt aggtttagct
     5941 atccaacgct atcaaaaagg ttttacaatt tctcctgtca ccatcgataa ggaaaagtat
     6001 gctgaagaat tggcagtagc tacaaagatt gctaggcgat tatcccatcg actggagcct
     6061 cagaataggt ttcctgagga agaagtggtc tacattgctc tacacttgat ttcaaaaaca
     6121 caattggagg tggagaagtc atcggaccgc ctcttgttgc gccaagcact ttttgaagtt
     6181 ttagggcgtt atgatgatga atatggttat caattttcac atgatttttc cttgatagaa
     6241 ggtttgctaa cacatctgga ggttctcgta gaacgactcc gtcacaatct ccatattgat
     6301 aaccccctac ttgatgatat taaaacaact tatcatgata tcttcgattt gacacaatca
     6361 ctaatggctg aactttccgt ttttcactct tatagtttgt ccgagtcaga aattgcctat
     6421 gtagctctac acttgatggc ttccttggaa cggcaaaaag aaaaatacaa actaaatgtt
     6481 ctggttatct gtgcaacagg ctacggtagt gctcagatgc tgaaaaatag gattaaaaat
     6541 gaacttgggc aacaggtgtc catatcagat gtcatcggtt actatgattt agataatcaa
     6601 aagttgcagg gggtagatgt gataatgtca accatcgact tatctggttt ggtgtttaag
     6661 gtccctgtta taactgtcag tgtcttttta acggatcagg aagttaagga agttaaacaa
     6721 cgattggcgg acttgagacc tagtctccaa aaaacacagg tagtggaaag agagagtaac
     6781 ttggagaaat actttgatca gtacttctca gaacaacgat ttttagttgt tgagtcagct
     6841 gaaaaagaaa tgttattaaa gcaaatgatt tcacagttgg agagcgaagg tcttggaaat
     6901 caccaggaat tttataaatt aattgaggcg cgtgaaagct tgagtacatt ggtatttgat
     6961 aaagatatag ctgttcctca ccccctaaaa cccattagcg aaaagcacat gatttctgtt
     7021 gcggttataa aaaatggtct atattgggaa gaaggctatg ggcagattag attggttttc
     7081 ttagtgagtc catctatata tacaaatgaa ggtctatcaa ccattaccaa tcgattggta
     7141 gatttggtag atttaccaga agtcaagaaa gacataataa actctcctga ttttgaaaca
     7201 ttcaaacacg tttttttaca aagagaatag aaggtgacat atgaatacag aagaattaca
     7261 aatggctgca tttgggatca tcctcagtag tggcaatgca agaagtgtta tccacgaagc
     7321 ttttgctgct atgagagaag gtgattatga taaggcagaa gagttattgg aagccgccaa
     7381 tgctgacatg cttgaagccc accattctca aacgggtctc ctacaggaat acgcaagcgg
     7441 tactgaagtg aaggtagaga ttattatggt tcatgcgcaa gatcatttga tgacaacaat
     7501 gactttacgt gaggtagcgc ttgaaatgtt agctttatat cgtaaagttg gttagaaaga
     7561 ggatatggga tgagtaaggg aatggcaagt aaaaagctac agaaaacacc agaacaatta
     7621 aaacaagagt ctaaacgcaa aagtcagctc tttaatcgct atatgctgtt tcgttatagt
     7681 ttagcagtgt tcttctttgc aaatctatac tggcttctgg ttcaattatt tcaaccaagt
     7741 ttgtatctaa tcctgccctc gcttatgttg attgcaataa tcattgcgac ggcagaacaa
     7801 tttaagttgt atagtgctga ggagtctcga ttgacaaaga cagaatttgt attaagggca
     7861 caagcaatct tgcaagggtt aattgtggtt ttagtgattt tgggtcatat ctcaaccctc
     7921 tttccaactt tttcaaacca tacatctgct aaactctttg ttattggctt gcagttgtta
     7981 ggtattgctt tagttgttct caatttaaaa aggttggagc aggtaaagca aaataccgat
     8041 aaatactacc tacgttttca aataataaga aaatatcttt aaaattttag gaggtcttct
     8101 catggaagaa caaaaagggt tctttggact attagataaa tatctgatgg gaccaatggg
     8161 taaactggca tcttacaaag ttgtacgtgc catcacagct gcaggtatgg ctgccgtacc
     8221 atttacgatt gttggttcga tgttcttggt tttcagtatc ttaccacagg cattttcatt
     8281 ctggccaatc gtttcagata tttttgcggc atcttttgat aaatttaccc cactctatat
     8341 ggttgccaat actgctacta tgggggtgct atcactttat tttgcacttg ctattggata
     8401 tgaattgaca aaaatttacg ctgaggaaga agaacttaat ttgactccgc ttaatggggc
     8461 gctcttatcc ctctttgctt tcatcatgac cgttcctcaa attatttttg aagaaggcgc
     8521 gatgtcggca gtcagctctc ttgaagaagg cgcagtaatt gttgatggtt gggcaatggg
     8581 tggtggcgta acacgctttg gtacaacagg tatatttacg gcaatcatca tggctgttgc
     8641 ggcagttctg ctatatcgga tgtgcgtgaa aaataattgg gttatcaaaa tgcccgattc
     8701 agtaccagag ggtgtatcac gcggatttac agctttgatt ccttcatttg tcgtagcctt
     8761 ctttgtaatt ttcttgaacg gtgtattggt tgcgcttggt actgatatat ttaaagttat
     8821 tgcaattcca tttagctttg ttggaaattt aacaaatact tggattggtt tagttattat
     8881 ctacttgttg actcaggcac tttgggtagt aggtattcac ggtgcaaata tcatctttgc
     8941 ttttgttagt ccaattgcac ttgcaaatat gtctcttaat gcagctggtg aacgaatgat
     9001 tgttgcaggg gagttctcaa atatgtttgt cattgcaggt ggttctggtg caacacttgg
     9061 gctatgtatt tggttagcta ctcgtgctcg ttctgaacaa ttgagtgcga ttggtaaagc
     9121 agcaattgtt ccgggtattt ttaatatcaa tgaaccatta attttcggtt taccaattat
     9181 ttataatcct gctctagcaa taccatttat gcttgcacca attgcatcta tgacggtata
     9241 ttacttctct atgaagttga atcttatcaa tgcagttgtt gctcaagtac catggccaac
     9301 tccagttggt attggtgcct tccttggtac agctgactgg cgtgcaattg ttgtttcaat
     9361 tgtttgtgca gttgttgcct tccttgttta ctatccattc attcgtgcat acgataaaca
     9421 gttgctaaaa gaagaagcgg caaatgctta aactagagaa actccttgac ttcttctaag
     9481 tccaaggagt ttttctataa gagaatatca ggagacattg ttagataaac ataagatagt
     9541 atcatatttg ctttcttctt tctaaatcag aggagctgag gtagggcaag agaagaagat
     9601 tttttatagc tgacttgatg aagcgactat caatacatat ggaggcttaa aaatatctct
     9661 actacttttg ctaatactga tcgagagaaa acctctccct tgttccaagt tttaattggg
     9721 atattatcta atgttttaag aatagtgata attggaaata gcatagaata gtatcagttt
     9781 ggcatattat cacgtaggaa tgaagggggt tcttttgcta ttatatagct aataaagtca
     9841 gaaggagtat tgtgctatga caaaacaatt tccaaaagga tttctttggg gcggtgcaac
     9901 agcggccaac caatatgaag gtggttggaa tttgggaggt cgtggtccag ccacttccga
     9961 tacctatata gcggtcgatc cagataagcg gaaggatatg agtcactttg gtaagcctgt
    10021 ctcgcgggct gatgtggaat ttgccttggc agatcaagaa ggactctacc caaaacgttg
    10081 gggttctgat ttctatcatc gctacaagga agacatcgcc ctctttgcag aaatgggctt
    10141 caagaccttc cgcttgtcga ttgcctggtc tcgtattttt ccaaaaggag acgagttaga
    10201 accgaatgaa gaaggactgg ccttttatga tgcggtcttc gatgagctga ataaatatgg
    10261 tatcgagcca ctggtgaccc tatctcacta tgaatttcct cttcatcttg ctctagaata
    10321 cggtggctgg aaaaaccgta aggtcataga gttctttgtt cgctatgccg agacagtatt
    10381 caaacgctat cagggtaaag ttaagtattg gctgactttt aatgagatca atattttggg
    10441 catggttggt tatctatcag gcggtcttct cttcgaagac ggaaagaatg acctagaagc
    10501 catgtaccaa gcagttcacc atcagtttat cgcttctagt ttggcaacaa aagctgccca
    10561 tgaaattgac ccagaaaaca aggttggttg catgctggca cggatggaga actatgcagc
    10621 cacctgcaat ccagacgatg tcttggcggc cttgaaaaaa gaccaggaaa atctcttcta
    10681 tacagatgtg caggtgcgtg gagcctaccc aagttatatg aaacgcttct tcaaagaaaa
    10741 caatatccag gtcgtgttcg agccgggaga cgaagcgatt ttgaaacaat atcctgttga
    10801 tttcatgagt ttttcttact atatgacatc catcacccgt gctcttccag acaaggacaa
    10861 ggcaacggca ggtaatttga tactaggaga ggccaatcct tatctggaag cgtcggactg
    10921 gggctggcag atagaccctg tcggacttcg tatcaccctc aacaagcttt atgaccgtta
    10981 ccaagttccc ctcttcatcg tggaaaatgg tctgggagcc ttggataagg tggaggcaga
    11041 cggcagtatt gaggacggct accgtattgc ctacctagaa aaacacatcc agcaaatgta
    11101 tgaagctatt gaagacggtg tggaacttat gggctatacc ccttggggct gtatcgatct
    11161 ggtttctgct tcaaccagtg agatgtccaa acgctacggc tttatctatg tggatgcgga
    11221 cgatcaagga aagggcagtt ttgaccgatc acggaaggca tcattctact ggtataaaga
    11281 tgtcattgcc agcaatggag ccaatgtatt gacagaaaaa taggaggtct tatgacaaaa
    11341 acgatttatc tcatgcgtca tggtgaaacc ctctttaata cgcaaaaacg agtgcagggt
    11401 tggtcggatt ctcccttgac agaacgtggt atcgctcagg cccaagccgt tggtcagtat
    11461 tttaaggagc agggaatcgt ctttaccagt gcctattctt ccacgcaaga gagggctact
    11521 gatacactaa aattagttac ggatgctccc taccaacagc tcaaggggct caaggagatg
    11581 aacttcggga tttttgaagc cagagaggaa gaattgcttc ccaagcaccg tccaggtgct
    11641 cgctctttcg aagacctctt ggttccattt ggaggcgagg atattcgagc agttggaagt
    11701 cgtgtcaaag aagcgattct ctctgtcgcg gaaagcgatc cagctgatcc aatcctcatg
    11761 gtcagtcatg gtgcagccat ttggggattg gtgcaggttt tggaggtcac cttcccacca
    11821 ggcgtcttcc tgcctaactg tgccatttgc cagctgactg ttgaaaatga gcaaatacga
    11881 tttgaaaaac tgattgattc cgcacatgat tttaaggttt atgattttaa gtaaggaggt
    11941 ttagtatgaa ctatctttac ttgatgcgcc atggacaaac tcggtttaat caacaaggtc
    12001 ggattcaagg ggcctgtgat tctcccctga cagaactggg aagagagcaa gcactagctg
    12061 ctcaccagta ttttcaagag caagggattg agttcgataa aatctattct tcaacccaag
    12121 aacgtgcctg tgatacggca gaattggtca cagggaggac ggattatatc cgcttgaaag
    12181 ggttgaaaga acaggatttc ggagccttcg agggtcaaca ggaatacctc aatcctccct
    12241 tacagggaga cattggctac ggtgattatt ttgtcacctt tggaggcgaa tcctatcaag
    12301 atgtccgaca acgaatggtc gaaaccattg ggggcatcat ggaagaagca gacaatcaat
    12361 ctgttttagt agttagccat ggagctgcca tagcccaatt tttcaggcag gtattgacag
    12421 attatccgca agttcggatg cgtaattgtg ccatactgac ttttacttat gaagacggtc
    12481 actacgattt gcaaagcatt gtggatcccg ttaataaaga aaatctctat caaaaagaag
    12541 gaaaggaaaa ggagaaaaaa tgacagtaac aaaagtattt ccaaaaggat ttttatgggg
    12601 tggtgcaaca gcagccaacc aatgtgaagg agcttacgat gtagacggac gtggacttgc
    12661 caatgttgac gtagttccaa ttggccaaga ccgagctgcc attatcactg gtcgtaagaa
    12721 gatgtttgac tttgaggacg gttatttcta tccagccaag gagtctattg atatgtatca
    12781 tcacttcaag gaggatattg ctctctttgg tgaaatgggc tttaagacct atcgtctttc
    12841 aatcgcctgg tcccgtattt tccctaaggg agacgaattg gagcctaatg aaaaaggcct
    12901 gcaattttac gaggacttgt tcaaggaatg tcacaagtat ggtattgagc cattggtgac
    12961 catcacccac tttgactgcc cgatgcactt gattgagaac tacggtggct ggcgaaatcg
    13021 taaaatgctt gaattttacg aacgcctttg ccgtaccctc ttcacacgct acaagggctt
    13081 ggtcaaatac tggctgacct tcaacgaaat caacatgatt ctccacgcac catttatggg
    13141 ggcaggtctg tattttgaag aaggcgaaaa tgtggaacag gttaaatacc aggctgccca
    13201 ccacgagctt gttgcctctg ccattgctac taaattagcc catgaaatcg atccggaaaa
    13261 caaggtcggt tgtatgttgg ctgcaggtca atactaccca cacacctgcc acccacgtga
    13321 tgtatgggaa gctctgcaag aagaccgtga gaactatttc tttatcgatg ttcaggcacg
    13381 tggctattat ccaaactatg ccaagaagaa gtgggagcgt gcaggtatcg agattgaaat
    13441 gaccaaccaa gacctagcac tcctgaaaga gcatacggtg gactttattt ccttctctta
    13501 ctactctagc cgtgtggctt ctggagatcc tgtagagaag gaaaaaacag caggcaatat
    13561 ctttgcttcc attaaaaatc cttacctaga cgcatcagaa tggggctggc agattgaccc
    13621 gctcggtttc cgcattacca tcaattccat atgggatcgc tatcaaaaac cactctttgt
    13681 ggttgaaaat ggtctgggag cagtcgacac gccagatgaa aacggctatg tggaagacga
    13741 ctaccgtatt gactatctcc gccaacatgt cttggcaatg agagatgcta ttgtcgaaga
    13801 cggtgttgaa ctcatgggct ataccacttg gggctgtatt gacctagtgt ctgccggaac
    13861 gggcgaaatg aaaaaacgct acggcttcat ctacgttgac cgtgacaatg agggaaatgg
    13921 cacactcaaa cgctctaaga aaaaatcatt tgaatggtat aaaaaagtca tcgctacaaa
    13981 cggagcggat gtgggataaa acacgagaga caggtcttcg gacttgtctt ttgttatcgt
    14041 ttccactata ataaaaatta gttaacaata aaggagtttt gcatgaaata catcgtaaac
    14101 aacagcaatg acccagctta taacatcgcc ctcgaagcct atgccttcaa ggaattaaca
    14161 gatattgacg aaatttttat tctctggatc aatgagcctg ccattattat cggtaagcat
    14221 cagaatgcta ttgaagaaat caacaaggaa ttcacagacg aaaagggcat ccatgtggtt
    14281 cgccgtctgt caggtggtgg cgctgtctat catgacctca ataacctcaa ctacaccatt
    14341 atctcaaata aggcagacga gggagccttt gacttcaaaa ccttctccaa gcctgtcatt
    14401 gatacccttg ctacactggg tgtagaggcc aatttcaccg gtcgtaatga ccttgaaatc
    14461 gacggaaaga aaatctgtgg taacgctcaa gcctatgcca aaggtcgcat gatgcaccac
    14521 ggttgcttgc tctttgatgt ggatatgtct gtattggcaa gtgccctcaa ggtcagcaag
    14581 gacaagattg aatccaaggg tgtcaagtca gtccgtgccc gtgtgaccaa tatcaacaac
    14641 gaattgcctg agaagatgac cgttctggag ttcaaggatg ctatcctcaa ccagatgaag
    14701 caagaatacc cagacatgga cgagtatgtc ttgtcagaag gtgacttggt tcgtatccaa
    14761 gaaatccgtg atacccagtt tgcgacatgg gattggacct acggtcaaac accagaatac
    14821 actgtggagc gtagcgtgcg ttacccagct ggtaaaatca caacctacat caaggctgaa
    14881 aaatcagtca tcgaatctat caagatttac ggagatttct tcggaattgg tgatgtatcg
    14941 gacatcgaag acttattggt cggtactcgc tacgagtatg cggatgttct tgccaaattg
    15001 caagaaatcg acaccacgca ctatttctca cgcatgacaa cagaagaagt agcaaaagcg
    15061 attgtagctt aatccatagg gctgcccttt gtttgggcag tctttatttt ttgtgaaaat
    15121 gttttcggca aactgttttc acttgcacat tttctatatt atggtataat tattttctgt
    15181 gagtatccct cacttactcg tggctagacc atgagtcatt agaccaaaag gaggaacata
    15241 tcaatggcta aatacgaaat tctttatatt attcgtccaa acattgaaga agaagctaaa
    15301 aacgctttgg tagcacgctt tgactctatc ttgactgaca acggtgcaac tatcgttgaa
    15361 tcaaaagcat gggaaaaacg tcgccttgca tacgaaatca aagatttccg cgaaggtttg
    15421 taccacatcg ttaacgttga agctaacaac gatgaagctc ttaaagagtt tgaccgtttg
    15481 tcaaaaatca acggcgacat tcttcgtcac atgattgtca aacttgacgc gtaagaaggt
    15541 gttgtatgat taataatgta gtattggttg gtcgtatgac ccgtgatgca gaacttcgtt
    15601 atactccgtc taatcaagct gttgcgactt ttactttggc ggttaaccgc aattttaaaa
    15661 atcaaaacgg tgagcgtgaa gcggacttta tcaacgtagt catttggcgt caacaagctg
    15721 agaatttggc gaattgggct aagaaaggtg ctctgattgg tgttacaggt cgtatccaga
    15781 cacgtagcta tgacaatcag caagggcaac gtgtctacgt tactgaggta gttgcagaaa
    15841 gtttccaact cttggaaagc cgtactgccc gtgaaggtca aggtggaggc tattcagctg
    15901 gcaactcgtt tgctggagga aatgattaca actcacctta tcaagcgcct gcacaatcta
    15961 caccaaactt cgctcgagaa gaaagtccat ttggagcaag taatccaatg gatatatcag
    16021 acgatgacct accattctag gtcaaaaatt aataatataa aggagaacac acatggctca
    16081 acaacgtcgt ggcggtttca aacgccgtaa aaaagttgac tatatcgcag ctaacaaaat
    16141 tgaatatgtt gattacaaag atactgagct tcttagccgt ttcatttctg aacgtggaaa
    16201 aatccttcca cgccgtgtaa ctggaacttc agctaaaaac caacgtaaag taacaacagc
    16261 tatcaaacgt gctcgcgtta tggctctttt accattcgta aacgaagatt aaaaaaatgg
    16321 tccagtggac cattttttca tgagctcgaa attaagaaag cgaattaagt ccttgaatgg
    16381 actgtttttt catgagcatg aaaataagaa agcgaattga gaccccgacg ggtcttttta
    16441 gcttgcgccc aagaaactgg aaaggcgcaa cggcgagagt ggaccatttt ttcatgaact
    16501 cgaaattaag aaagcgaatt aagcccttga atggactgtt tcaacccgag ccaagaagtt
    16561 agaaagcgag gcacagtctg gggatagact gtttcagcag attacataga caagtgacag
    16621 cggataaagt tctcgggcga tttgatagct ggactgtata gataaacaaa agcgactgga
    16681 atattccaat cgctttgttt tattattgaa ctggtgacat ggcattgcga atgttatatt
    16741 tctttttcaa gaggtagcgt gcaccgaaag cagcagctcc gataagaata gtaacaagcg
    16801 gagggagaac tgggttaaaa gcggccggaa tcagtgctgc cagaatgaaa acgactgacc
    16861 aagccagcgt agcaagtcca agaaaagcga tagctttcag caatttagga cgttgcccag
    16921 ttttgatttg ttctctgtaa acgaaatggt acatcaagta catccctgca ccaacaccga
    16981 aaccgatgac aaataaggtt aatagcccat actgtgcacc ttgaccaaaa agattcatca
    17041 aaccattgat accagcaata attgcaagca tgaaaagact agaatctagc cacatcaacc
    17101 atggattttc attgtattcc acttggtctt tcttttcctg ttcagaaact gtcttactag
    17161 cagcccactc acttggtgct ccatagagat tgcgggcagt cacgccagtt ttttgctttt
    17221 caatgatagt tggtaaaatt tcttccaaga gttctttgat ttcgctatca gactttccat
    17281 ctttaatcag ttggttggtc gcaatatgga taaattcttg gttctttttt gttaatccgt
    17341 tgatttctgt aaatgacatg ttaatctcct taggttaaaa ccatctctta cggatgaagt
    17401 agaaggtgag tgataggctg attgcaaatg cgaagaggat aatccaccaa aaagcatagg
    17461 gaatatcgtt aaaaggaatg atgttgtcct taaagttcat accgtatgca gagaagacca
    17521 tggttgggat agagagaacg attgtcacta atgccaaggt tttcatgatg gtattctggt
    17581 tgttagaaat gatagaagca aaggcatggg tcatagagtt caagatggaa ccatagatgt
    17641 ctgccatctc aatggcctgt tgggtttcga ccaaggtatc ttcaagcaaa tcctcatcct
    17701 cttcatactt acggagtaga cgtgaggaac tagcaagttt tttgaccaaa cgctcattgg
    17761 ttttgaggga agccttgaag tagacgatgg ttttttccaa ttccatgagt acaattaatt
    17821 cttcatttcg agtggcctca tggagttgct tctcaatctc ttcgctcttt ctgtcaatgg
    17881 tccgtagggc tgacaagtag agttgagcat tgcggtagag aatttggaaa acgaaacgag
    17941 acttcatgaa agtgaagaaa ttccgtagac gacgatggat aaactgctca aacagtggca
    18001 atggttccaa acaagttgtg ataatcgcat catctgttag aataattccc aaaggaatag
    18061 taatataata tgtcttgttg tttcgttctt caagaattgg gacgtcgacc aggatgagcg
    18121 tatagtcatc ttcgacggtg atacgagagg attcttccgc atcgagcggt gctcgcaggt
    18181 cagcgatgtc aattttatag tgctcagcca cttccataga ctcgctctgg gaggggttga
    18241 ccaaattaat ccaagcccct ggttcaaagg tttggatttc ttctaaatca gtcatggttg
    18301 atagaaaaat ttgtttcata tctttgccct ccttttatct ttgttgagtt aacgcaacct
    18361 ctattatacc aaaaaatgtg cagtttcgga aaggttttat atagaaatgt gatataatat
    18421 gtagacattt aagcgaataa gttgtacgga cttttccttg ttccgtatac catagcttat
    18481 agcctcttaa gaaaggaatt gaacacgggc taaatgcggt gtcaaaaatt gctggtttct
    18541 tcatgtttca gcatggaaac tggcttttgc gtcgtagaca tctgtgcttt cttcggttat
    18601 caacctgtga aagcactagt tgtcttgacg ggggtgcgtc ttcggaatca cagatttcgg
    18661 acttaccgcc tattttgacg gtgcgtttta cggtctttgt atctttagaa aggaaatgaa
    18721 cacgggctaa atgcagtgtc aaaaattgct ggtttcttca tgtatgagta tggggaatta
    18781 gcttctgcgt cgtagacatc tgtgctttct tcggttttcg acctgtgaaa gcactagttg
    18841 tcttgacggg ggtgcgtctt cgaaatcaca gatttcggac ttaccgccta ttttgactat
    18901 gcatttgacg ccctttgtat cttagaaagg attcgaatgc gtgctaaaag cgtcgagaaa
    18961 aagacaaaat gttttgtcac tgatgtgacg tcacattttc ctattttctt ctcgctttct
    19021 taacgcactt atatctagaa aggaattgaa cacgccctaa acgctgtgtg aaaaagacaa
    19081 attctcctag agcgttaacg ctcttcgtca aatttcctat tttcaccttg cgttttacgg
    19141 gctctgtatc ttaaattatg caagaaaata ttgtgattca tggggcgcgt gcccataatt
    19201 tgaaaaatat tgatgtgacc attccgcgtg agaagctggt ggtggtgact ggtttgtcgg
    19261 ggtctggtaa gtcgagtttg gcttttgata ctctgtatgc cgaaggccaa cgtcgctatg
    19321 tggagtcttt gtctgcctat gctcgtcagt tcttgggcaa catggacaag ccagatgtgg
    19381 attccataga ggggctcagc cctgctattt ccattgacca aaaaacgacg tcgcggaatc
    19441 cgcgttcgac agtcggtaca gcgacggaaa tcaatgacta tctccgcctg ctctatgctc
    19501 gggtcggggt gccgtactgt atcaatgggc acggggcgat tgcggcttct tcggtggagc
    19561 agatcgtcga tgaggtcttg gaattgccag aacgccagcg tttgcagatt ctagctccga
    19621 ttgttcgtaa gaaaaagggg caacacaaga ccatttttga gaaggtgcag aaggacggct
    19681 atgttcgggt tcgtgttaat ggtgatgtct atgatgtgtc ggaagtacca gaattgtcca
    19741 agagcaaggc tcataatatt gaagtcgtgg tcgatcggat tgtgatcaaa gagggcattc
    19801 gctcgcgact ttttgattct atcgaagcgg ccctgcggat tgcggacggt tatgtcatca
    19861 ttgataccat ggatgacaag gagctgcttt tctctgagta ctatgcctgt ccagtttgcg
    19921 gctttacagt gcctgagttg gagcctcgcc tcttctcttt caatgctcct tttgggtctt
    19981 gtagcgactg tgacggtttg ggcatgaagc tggaagtgga tacggatttg attgtcccag
    20041 atgccagcaa gaccttgcgt gacggggctt tggctccgtg gaatcctatt tcttctaact
    20101 actaccctca gatgttggag caggccatga accattttgg tgtggacatg gacaagcctt
    20161 ttgaggaatt gacggaagag gagaagaacc tgattttcaa cggttctgac gggaaagaat
    20221 ttcatttcca ttatgaaaat gagttcggtg gggtgcgtga tatcgatatt ccctttgaag
    20281 gtttgattac caatatcaac cgtcgctatc gtgaaaccaa tagtgattac acacggacag
    20341 tcatgaagac ttacatgaat gaattgacct gtggaacctg ccacggctat cgcctcaatg
    20401 accaagccct gtctgtcaaa gttggtggcg agcagggact tcatatcggg cagttgtctg
    20461 atttgtccgt agcggatcat ctggaagtca ttgaacatct gaccttgtct gaaaatgaag
    20521 ccaccattgc aacacccatt gtcaaggaaa tcaaggaccg cctttccttc ctaaataacg
    20581 ttggtctcaa ctatctgact ctttcacgag cagcaggtac cctttctggt ggtgagagcc
    20641 agcggattcg tctggcgact cagattggct ctaacctgtc tggtgtcctc tatattctgg
    20701 atgaaccgtc gattggtctg caccagcggg acaatgatcg tctcattgcc agcttgaaaa
    20761 agatgcgtga tttaggcaat accttgattg tggtggagca tgatgaggac accatgcgtg
    20821 aggcggactg gttgattgac atcggtccag gtgcgggtgt ctttggtgga gaaatcgtgg
    20881 cttctggaac gcctgcccaa gtagctaaaa ataaaaaatc cattacaggc cagtacctgt
    20941 ctggcaaacg tgaaattcca gttcctctag aacgtcgtgt tggcaatggt cgtttcctgg
    21001 aggtaacagg tgccaaggaa aataacctgc aagatgtgac tgttcgtttc ccgctcggaa
    21061 aatttgtagc ggtgacaggg gtgtctggct caggcaaatc aaccctggtc aattccattt
    21121 tgaaaaaagc tattgctcag aaactcaatc gcaattcgga caagccaggc aagttcaagt
    21181 ccatttcagg catcgaacac ttggaccgcc tgattgacat tgatcaaagc ccgattggac
    21241 gaacaccgcg ttccaaccca gctacttata caggtgtttt tgacgatatt cgggacctct
    21301 ttgctcagac taatgaggct aaaatccgtg gctacaagaa gggacggttc tcctttaacg
    21361 tcaaaggtgg tcgttgtgag gcttgttctg gtgatggtat catcaagatt gaaatgcact
    21421 tcctgccaga tgtctttgta ccttgtgaag tctgtcatgg acaccgctac aattctgaaa
    21481 ccttggaagt acattacaag gagaaaaata tcgcccaggt ccttgatatg acggtcaatg
    21541 atgcggtcga gttcttcaaa catattccga aaattgagcg gaaacttcgc accattcaag
    21601 atgtaggctt gggctatgtt acccttggac aacctgcgac aaccctatca ggtggggagg
    21661 cccagcggat gaaattggca tcggaacttc acaagcggtc gactggtaag tccctctata
    21721 tcttggatga gccaacgaca ggtctacata ctgaggatat tgcccaattg ctcaaggtct
    21781 tggcccgctt tgttgatgac ggcaatactg tccttgttat cgagcacaat ctggatgtca
    21841 tcaagacagc cgaccacatt atcgacatgg gaccagaagg cggtgtcggt ggtggtaccg
    21901 ttgttgcaac aggaacgcca gaagaagtag ctagcaaccc agccagcttt acaggccagt
    21961 atctgaaaat gaagttgaaa taaaaagccg cctctgcggc tttttttctc tatctgctag
    22021 gaagaattgg tatttgtctg tatttctgct ataatagtta caaaatcttt ggaggtagct
    22081 tatgcaagcg cgtgtggaaa aatttgaggc aaaattagcc caatctgatg tggatggaat
    22141 cttggtgact ggtcaaaaca atatttacta cctgacaggt ttttggggga cagaggcgac
    22201 tgtcttcatc agtggcaagc gtcgcttgtt tgtgactgat tcgcgttata ccttgatagc
    22261 caaggcatca gtaaaaggtt ttgatattat cgagagccgt tttgcccttg aagagattgc
    22321 aaaggtgatt aaggaagatg ggcttgaaaa aattggtttt gatagcgagg tgacctatgg
    22381 tttctatcaa agtctgacca gcatttttga gggttatcaa ctggttgcta tgtcaaactt
    22441 tatcgaagac ttgcgcatga ttaaagatga gaaagaaatt gcgaccattc gccgtgcatg
    22501 tcaaatttcg gatcaggctt ttattgatgt tcttgacttt atcaaaccag gtcagacgac
    22561 tgagatggac gtcaatcatt tccttgacca ccgcatgcgt caactcggtg cagaaggggc
    22621 atcatttgag ttcatcgtgg catctggcta ccgctctgct atgcctcatg gaagagcgtc
    22681 tgaaaaggtt atccaagctg gtgagacctt gacgctggat tttggttgct attaccagca
    22741 ttatgtcagc gatatgacac ggaccatcca tatcggtcat gtgacagatc aagagcgtga
    22801 gatttacgat gtggtcttgc gtgccaataa ggctttgatt gagcaggcca aggaaggtgt
    22861 cacctaccgc gagtttgacg ctattcctcg tgagattatc aatgcggcgg gttacggagc
    22921 caactttaca cacggcatcg gtcacggtat cggcttggat attcatgaat atccgtattt
    22981 tggaaaatct gacgagacta tcaaggcagg tatggtcttg acagatgagc ctggtatcta
    23041 tttggatgac aaatatggtg tccgtatcga agatgaccta ttgattacgg aaaacggttg
    23101 tgaagtcttg acattagctc ccaaggaatt gattgtcatt tgagaaattt gacagtttgt
    23161 ggtagaataa agcgaataaa taaaatttga agaggtattc aatacaatga ttgaagcaag
    23221 taaacttaga gcaggtatga ccttcgaagc tgaaggtaaa ttgattaaag ttttggatgc
    23281 aagccaccac aaaccaggta aaggaaacac catcatgcgt atgaaattgc gtgatgtacg
    23341 tacaggttca accttcgata caacttatcg tccagatgaa aaatttgaac aagctattat
    23401 cgaaacagtt ccagcacagt acctttacca aatggatgac actgcttatt tcatgaatac
    23461 tgagacttac gatcagtatg aaattccagt agcgactatc aaagaagaat tgctctatat
    23521 cttggagaat tccgatgtga agatccagtt ctacggaaca gaagtgatcg gtgttactgt
    23581 accaacaact gttgaattgg tcgttgcaga aacacaacca tctatcaaag gtgcgactgt
    23641 tacaggttca ggtaaaccag ctacgatgga aacaggtctt gtagtcaacg taccagactt
    23701 tatcgaagca ggtcaaaaac tcgttatcaa cacagctgaa ggaacttacg tatcacgtgc
    23761 ttaatcgttt cctcttaaga aaggagagcc tatgacaaca gaatatcaag gcgagattgt
    23821 tatcgcacct cgcgtccttg aagtaattac aggtattgct gcagcgaaag ttgatggagt
    23881 gcattccctt caaaataagc gtgtagcaga cagtttgtca aaaacatcgt taaataaagg
    23941 tgtttatctt gaaactgacg aggaaggtcg agtgactgcg gatatttacg tctatctaga
    24001 atatggtgta aatgttcctg ccgtatcaat ggacatccaa cgtgcagtca agacagcagt
    24061 ttataactat gcagaagttc aagtggctgc ggttaatatc catgtcagcg gcattgttcc
    24121 tgataaaaca ccaaaaccag cattgaaaga tttattcgga gaggatttcc ttgatgaaga
    24181 ataatagaca tgccctgcgt aagtgtgctt tgcaggcaat cgtatcactt gaatttggtc
    24241 aagaacctgt tcaggcagct cagttttctt atctttatga tagagaggaa gagcaagaag
    24301 gagttgaaat tccgctcttt ctccttaacc ttgtcaatgg tgtagcagac tatcgtgatg
    24361 acttggataa ggagttatct agccgattaa aagcaggatg gaccttagac cgtttaacct
    24421 tgattgacaa gaatatcatg cgactaggcc tatttgaaat cttacatttc gaagaaacgc
    24481 cagaccgcgt tgctgttaac gaggctattg aattggctaa agaattttct gatgaatctt
    24541 cagctaagtt tgtcaatgga gttttgagcc agtttattaa agaagaagcc tgatgagctt
    24601 cttttttact tgagaaacaa gagaatgtat cacaaaaaaa ctgcacctaa tttttattag
    24661 gtacagtttt tttatatact agctcctgct agtaggctaa tcggtaggat atagtctgtt
    24721 tttgtcagtt tttttccttc gattttatca actaatagtt caaccagcag gcgagctatt
    24781 tcggttaatg gttgcttgat ggttgtcagt tgaggatagt agttttcgat gaaactggtt
    24841 ccatcataac caatcaattt aatatcttct ggaatagaca gtttcattga acgaatgacg
    24901 ttccatacca gcatagccgt tgcatcgcca gatacaaaga taccatctgg tttttgcttt
    24961 tcaagaatgg ctcggatttc catctccttg cgcacaggtg agaaatcact ggagatatga
    25021 aaaacttcgg ctttgttcaa aatagatgtg aaacctactt ggcgaaggcc ggtaggtgaa
    25081 ttggaatcat cgttccccgt aatcattaat ggattgtgac atcccgcttt ttgtaaggtt
    25141 tgggcagcta ataccccgcc agcaaaatta tcggaagaga caatggggat ggaaggagag
    25201 aggttgcggt caaaagcgat aattggagct acgacacggt cgtagtcttg gataccgaga
    25261 ttgtgactgc ccgagataat cccatctact tggttagctg ctaacatttc tagatactca
    25321 cgttccttat cggactcgtg ctggctattg caaataatgg ttttatagcc tcgatcaaaa
    25381 agctcttttt ctaaatggct aatcaattca gaataaaaaa tattgtttat tgttgggaag
    25441 atgagaccaa ctaattttgc agatttacct tgcaaactac gagctaaatt attaggtttg
    25501 tagcccagtt cacgcatagc ctcttcgaca ttgcgaatgg ttttttcaga taggtatcct
    25561 tttttattga taacgcgcga gacagttgtt gggctgaccc cagctaactg tgcgacatca
    25621 gttagttttg cgaccataat ctaaatggta gtaggttccg gtcggattac cagtcgtaat
    25681 gacgatacca gactgatccg catcagggaa gacacgacca gaaaatactt tctctccttt
    25741 attgataaag atttcaaaga cagatttgtc gatgaagata ttggctgtga gagctcctgg
    25801 agaaatctcg caagagcgac ttgtaccgaa atcgagtgcg aatggctgac cacagttgcc
    25861 gcggtccaat gtaatctggc cttgttcagt gtccacagac aagaccaagc ctttttctgc
    25921 ttcatttgcg aacaatacga tttcgtgctt cgtgcccgct gaaaaatcaa gttccaattc
    25981 gtaaacatta ttagtttctt tttgggctga aaatggctga gcttctttac gaagactggt
    26041 gatcgcatca actggatact ggtagagctt accgtccttg atggttaatt ctttaaccaa
    26101 tgacagacag ccttggtagt cataagcatc ggttgggtat tccacatctg gaagtgccaa
    26161 ccagctaaca gatagagttc gaccgtctgg tgcattaaag gcctgggttg catagcactc
    26221 aaaaccatag tccagattga tcaattcgct cggttcgaca atgcttgctg tttcaggatc
    26281 gaaactctga ccaatcttgt acatatttgg atagatattg ctatagtcca atacctcttt
    26341 gtccaagcct tgtggacagt agagaaggat gggttgctca ccgacgaacg caatatttgg
    26401 gcattccatc atataagcgg tcatatcatt ggcaaaatca aggtcaccaa cataggacca
    26461 gttgaggtag tcgttatttt cagctttata aagtttgaca attccctttt tatccagatt
    26521 ttgtccaccg acaatagcgt aatattgccc tttatagttg aaaatttgtg gatcacggaa
    26581 atggtccgtg gtatcttctg gttgctcaat caagacttta tcaatctttt caatcttacc
    26641 atctttatcc atcaaggcac cgatttggta tggatggcga acccaatttt catcgcggac
    26701 atttccagta tagaagagga agagcttgtc gccaaattcc atggcagatc cagagtaggc
    26761 accgtggcta tcaagtggtg tatctgggta cataaccgta ccagtttttt caaaatggac
    26821 caaatccgtt gattctgtat ggacccaaga ttttaatccg tgcgcagcac cgaatgggaa
    26881 gtattggtaa aaaagagtcc actttccatt aaaataagaa aatccatttg gatcatttaa
    26941 cagtccatgt ggtgtttcaa tatggaaatt tgtcctccaa ggagaattct ctgtattctt
    27001 ctttattttt tcaatatatt cgggtgtcca gtcagcataa gctctgtaac gctcctctgt
    27061 cgtaaatgcc ataattttct c
//
LOCUS       DBNFEG010000009        26590 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.009, whole genome shotgun sequence.
ACCESSION   DBNFEG010000009 DBNFEG010000000
VERSION     DBNFEG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 26590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26590
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.009"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..644
                     /locus_tag="AC1PNG_000243"
     CDS             <1..644
                     /locus_tag="AC1PNG_000243"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047465 - N-acylglucosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HIP6700782.1"
                     /translation="CQALPGEPLYREEGGIMPLLVKAAQEAGAVGIRANSVRDIKEIK
                     EVTTLPIIGIIKRDYPPQEPFITATMREVDELAALDIEVIALDCTKRERYDGLDIVDF
                     INQIKEKYPEQLFMADISTFEEGLTAYEAGIDFIGTTLSGYTSYSRQEEGPDIELVDR
                     LCRAGIDVIAEGKIHYPDQVKIIHDLGVAGIVVGGAITRPKEIAERFISALHK"
     gene            672..2180
                     /locus_tag="AC1PNG_000244"
     CDS             672..2180
                     /locus_tag="AC1PNG_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIP6700783.1"
                     /translation="MNFKKFSQLGQAFMLPISILPVAGLLLGIGGALSNSNAVAQFPV
                     LDQAWLQAVFTIMSTAGSAVFANLALIFAIGVAVGLADADKGTAGLSAGVAYLVYTAT
                     ISGFLTLFADKGASIDTGVLGALAIGITVATLHNKYRKIELPAFLGFFGGSRFIPIIS
                     SFAAIAIGSIFYIIWPPIQNLLVDFGKAIAEMGSFGTFLYGFGLRLTGAFGLHHTIYP
                     MFWYTSLGGSEVVAGETIQGAQNIFFAQLADANHTGLFTYGTRFFAGRFATMIFGLPA
                     SCLAMYHAIPKENRKKVGSFYSSSALTSLLTGITEPIEFSFLFVAPWLYVVHAFLDGL
                     SFLVADLLQIRIGNSFSGGLIDYLIFGVFQGNDKTNWIYVIPVGLLWAAVYYLVFKFL
                     VTKFHVLVPGMESSDQASHSEKVLSNGSSLRDNAIAIIDALGGADNIENVTACATRLR
                     VSLRNSGLVDKGVIQSIGATAVLDVKGGIQAIFGGKAILYSQEINQILGLEE"
     gene            complement(2284..3096)
                     /locus_tag="AC1PNG_000245"
     CDS             complement(2284..3096)
                     /locus_tag="AC1PNG_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIP6700784.1"
                     /translation="MLQTPKHITAIIESHLDQMTRLEKQIASYFTQLDPSSADLSQEN
                     TIQQLHISPSALTRFAKKCGFSGYREFVFAFQNNQQYLDKHFEKLQRSLTKKVLVDYE
                     EILTATNKLVDEEKLEEIAKLIDSSKRVYFYGIGSSGLVAMEIKSRFMRLGVVCDAIT
                     DENNLIWTTNILDDSCLVIGLSLSGQTEEVMEHLQLAAQKGIPTALLTTKSFPHPTFD
                     QIIPVASVRHLNYGNRISPQIPLLIMLDIIYAYFLAIDKEKKENIFKQTIRE"
     gene            3196..3693
                     /gene="ybeY"
                     /locus_tag="AC1PNG_000246"
     CDS             3196..3693
                     /gene="ybeY"
                     /locus_tag="AC1PNG_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIP6700785.1"
                     /translation="MYIEMIDETGQVSAQMQEQITELLQFAAEKIGKQNKEMAVTFVD
                     NKRVHEVNLEYRGIDRPTDVVSLEYKPESEIVFDEEDLLDNPELAEMMEDFDAYIGEL
                     YISIDKAREQAEDYGHSYEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRE"
     gene            3674..4075
                     /locus_tag="AC1PNG_000247"
     CDS             3674..4075
                     /locus_tag="AC1PNG_000247"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HIP6700786.1"
                     /translation="MDLQENNSKNRWKNRELIASLEFAVTGLLTAFKEERNMKKHLAS
                     AILVVLAGLIFQVSVTEWLFLLLSISLVIAFEIVNSAIENVVDLASDYHFSMLAKNAK
                     DMAAGAVLFVSGFALLTGLIIFVPKIWDFIF"
     gene            4092..4991
                     /gene="era"
                     /locus_tag="AC1PNG_000248"
     CDS             4092..4991
                     /gene="era"
                     /locus_tag="AC1PNG_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIP6700787.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNYVMGQKIAIMSDKAQTTRNKI
                     MGIYTTEEEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEKRGKGD
                     DMIMERLKQAKVPVILVVNKIDKVHPDQLLEQIDDFRQQMDFKEIVPISATQGNNVNR
                     LMEILKENLDEGFQYFPADQITDHPERFLVSEMIREKVLHLTREEIPHSVAVVIESMK
                     RDEFTDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
     gene            5679..6506
                     /gene="mutM"
                     /locus_tag="AC1PNG_000249"
     CDS             5679..6506
                     /gene="mutM"
                     /locus_tag="AC1PNG_000249"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HIP6700788.1"
                     /translation="MPELPEVETVRRGLNRLVKGKIISKVEVTYAPMIKTGVDAFCQD
                     LIGQEILDVDRRGKYLLIYLTDHVLISHLRMEGKYNFFPDQVPANKHFHAFFTFSDGS
                     TLVYQDVRKFGTMELLGKADVDAYFISRKIGPEPTEEDFDLEEFAKKLAKSRKPIKSH
                     LLDQSLVAGLGNIYVDEVLFKAKVHPAQTSNQLSAEQVADLRQATIEVLQLGIEKGGS
                     TIRTYKNALGMDGTMQDYLQVYGKTGQACPRCQTEIVKIQLGGRGTHFCPKCQVKHG"
     gene            6499..7101
                     /gene="coaE"
                     /locus_tag="AC1PNG_000250"
     CDS             6499..7101
                     /gene="coaE"
                     /locus_tag="AC1PNG_000250"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637643.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIP6700789.1"
                     /translation="MAKIIGLTGGIASGKSMVTAFLREQGYSVIDADAVVHELQAKGG
                     KLYQVLVKEFGQDILSDDGNLDRAKLGQAVFADSKLRARLSDLQDQIIRQELLDRRDA
                     LKQTEDVIFMDIPLLYEADYSGEVNEVWLVYVDRAQQLERLMKRNGLAVQDAENRMAA
                     QLSLEEKRSQAQVVIDNSGAVEATLVQVARLLEELKDGRR"
     gene            7088..8293
                     /locus_tag="AC1PNG_000251"
     CDS             7088..8293
                     /locus_tag="AC1PNG_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HIP6700790.1"
                     /translation="MDDGSSYWKQNLKVAWLGNFLTGTSFTLVMPFISVFVEELGVGP
                     GQVEYYAGLAVSVNALAAALMAPIWGSLADRYGRKPMMVRAAFAMIFTMGGMAFVPNV
                     FWLLVLRVLNGVFTGYIPNATALIASQVPKDKTGYALGTLSTGAVAGNLIGPTLGGIL
                     AEMFGVHMVFLLVGLLYAIVVLLTVFYIREDFVPIKKGEEMSVKEVFEQVKDRQMLVG
                     LFVTSMIIIAAAQAVVPILTLYVRHLGQTDNLLFVAGFIISLPGMASLVTSGYLGKIG
                     DHIGNHRLLLIALTYSLLINVFCVFAENPFQLGLLRFMYGFGTGALLPSVNSLLTKLT
                     PKEGISRIFSYNQLFNNLGSVVGPMMGSAVAAHMGYDWVFYLSSGLVLFNLIWSLTNF
                     RNYLKVRDV"
     gene            8293..8442
                     /gene="rpmG"
                     /locus_tag="AC1PNG_000252"
     CDS             8293..8442
                     /gene="rpmG"
                     /locus_tag="AC1PNG_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIP6700791.1"
                     /translation="MRVKINLQCSECGSKNYLTSKNAKTHPDKIEVLKYCPKERKVTL
                     HLETK"
     gene            8489..8722
                     /gene="secG"
                     /locus_tag="AC1PNG_000253"
     CDS             8489..8722
                     /gene="secG"
                     /locus_tag="AC1PNG_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIP6700792.1"
                     /translation="MYQALLAILLILSVILIVVIFIQPAKNQSSNVFDSSSGALFERT
                     KARGFEAVMQRITAILVFLWMLDALALVIISSK"
     gene            9153..11522
                     /gene="rnr"
                     /locus_tag="AC1PNG_000254"
     CDS             9153..11522
                     /gene="rnr"
                     /locus_tag="AC1PNG_000254"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIP6700793.1"
                     /translation="MKNDIINYIKEVGPVTMDQLADQFGASSAKSFTDLVKLVSSMEG
                     SRQLVFDNAGRITLPAPKISKNKVTLQGIFRAHKSGFGFVTIDEEEDDLFVSRDDVNF
                     AIEGDRVEIAIKKVADRLKGTAAEAEVIDILEHSLKTAVGLIVFDEDKPEYAGYIKSK
                     NQKIAQKIYIKKSPLVLTGTEILKVDIEAYPNKKRDHFVATIRDVVGHKDDVGIDVLE
                     VLESMDIVSEFPDEVLAEANRVPESPSEQDFAGRLDLRDEIIFTIDGADAKDLDDAVH
                     IKQLKNGNLELGVHIADVSYYVTEGSALDQEAIKRGTSVYVTDRVVPMLPERLSNGIC
                     SLNPNVDRLTQSAIMEIDRKGKVVKHWIGQTVIKTNFRMTYSDVNDMIAGNQEKLDKY
                     KTIVPSVELMVKLHETLETMRYKRGALNFDTAEAKIIVNKDGLPVDIQLRQRGIAERM
                     IESFMLVANECVAEHFAKLDLPFIYRIHEEPKSDKLQKFIDYATSFGLTVYGTASSIS
                     QDALQDLMERVKDEPYADVLNMMLLRSMQQARYSEHNHGHYGLGAEFYTHFTSPIRRY
                     PDLLVHRMVRDYGHNPAEKAEHFEQVIPELAKSSSSLERRAIEAEREVEAMKKAEFMQ
                     EFVGQEFDGVVSSVVKFGLFVELPNTVEGLIHITNLNEYYQFHERTLTLQGEKSGRVF
                     RVGQPIRIKLTRADKMTGEIDFAHVPSELDIVEKALKAKRRTASHSNRDRDDRSGRGR
                     GKHHKQEAAGRDSGRHKSGKDHKSKSGKKDKKKKPFYKEVAKKNKKKRR"
     gene            11525..11992
                     /gene="smpB"
                     /locus_tag="AC1PNG_000255"
     CDS             11525..11992
                     /gene="smpB"
                     /locus_tag="AC1PNG_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIP6700794.1"
                     /translation="MAKGEGNVIAQNKKARHDYTIVDTVEAGMVLTGTEIKSIRAGRI
                     NLKDGFAQIKQGEAWLVNVHIAPYDEGNIWNQEPTRTRKLLLRKKQIESLGNEVKGTG
                     MTLVPLKVYIKDGFAKVLIGLAKGKHDYDKRESIKRREQDRDIKRTMKAINSR"
     gene            complement(12079..13122)
                     /locus_tag="AC1PNG_000256"
     CDS             complement(12079..13122)
                     /locus_tag="AC1PNG_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIP6700795.1"
                     /translation="MKTAIYEKSGKMTVAEIEKPSLQAKDDVIIKVVRACVCGSDLWG
                     YGEDIHHDHGDTNSGHEAIGIVEEVGEDITTLVPGDFVIVPFTHGCGHCDACRAGFDG
                     TCDNHPGYTNWGNNYQSQYIRFHYGNWALVKIPGQPSDYSEGMIKSLLTLADVMPTGY
                     HADRVADVKPGDKVVVIGDGAVGQCAVIAAKMRGASQIVMMSRHTDRQQMALESGATA
                     IVAERGEEGIAKVREILGGGADAALECVGTEASIDQALGVLHNGGRIGFVGVPHYNNH
                     ALGSTFAQNIIIGGGSASVTTYDKEILLDAVLKGDINPGRVFTQTYSLDNIDQAYKDM
                     ANRKTIKAMITVD"
     gene            13276..14136
                     /gene="tehB"
                     /locus_tag="AC1PNG_000257"
     CDS             13276..14136
                     /gene="tehB"
                     /locus_tag="AC1PNG_000257"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HIP6700796.1"
                     /translation="MTVLVPYKRMPIWNQDTVPQHFLTKHNTKVGTWAKIKVLRGQLK
                     FEPISDDNEILGEYVYGPTADIPFVEPQAWHRVTLLTEDTEFFLEFFCQPEDYFAKKY
                     DYTRTHSEVLEALKRIEPCKVLDLGCGHGRNSLYLAQQGFEVTAVDKDVPSIRTLLQV
                     KEVEDLDLQAGTYDINSASLEQDYDWIISTVVFMFLERDRVPAIIRNMQEQTRPGGYN
                     LIVAAMDTEDAPCPMNFSFTFGVGELKEYYRDWELVKYNEDFGQLHKRDENGHFLKMR
                     FATMLARKKG"
     gene            14330..16153
                     /locus_tag="AC1PNG_000258"
     CDS             14330..16153
                     /locus_tag="AC1PNG_000258"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HIP6700797.1"
                     /translation="MKKTFVRIATFLMLVCLFPVQLVQACSGFIIGKGLTTDGSILYG
                     RTEDYPYPPDNGAHNKNYIVVPAAAYAKGDMLVDESFGFTAPHLANEFKYTSTPDAAR
                     GDGSNGNFGAHGFNEKGVSMTATVTAIPNKKVLAVDPLVTAGGLGEAILIDYVLPRVT
                     SAREGIELIAKTIDEKGSAEGNIIVIADKNEVWYMEILSGHQYVAIKFPEDKYAIFAN
                     TYYLGHVDFTDTENVIASAKVEEVAKQAENYMMVDGKFHIAKSYGPENYADGDRSRTY
                     AGIKLLDPASSVTYEDAVYDLLRQPTDPSRRFSLQDTFALQRNRFEHLPEFRPDDEAG
                     KVKQGDNGANDQAADATYKYALGNENVIDAHVYQINSSLPSAFGGTVWLGLAQTRNTP
                     YVPFYGIVTDTYEAFKNRSASYDTNSWYWTVQNIDKMAISHPELFGTSIQEKWIALEK
                     EWIASQAALDAQYAGLSEDAAVALAPTVTEATLARSAEIFAQLKAVEAEMMAKIEAAT
                     TPSSSSTEPSTSTEPSSSGTETSTSTSQSTSDSNTGGATDTSSSSGTVVPSDKKVTPT
                     NKKGKSNLPSTGEQVSILLVALGVAGILTAIFLHRKKSSKE"
     gene            16274..18724
                     /locus_tag="AC1PNG_000259"
     CDS             16274..18724
                     /locus_tag="AC1PNG_000259"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIP6700798.1"
                     /translation="MKQASYPIQGMTCASCALTVEKAVGKLIGIEEASVNLATEKLSV
                     SYDEKLLGLEDIRQAVEKAGYQLVDNLVTESYDISGMTCASCALTVEKALGKLEGVEE
                     VSVNLATEKATIRYSRHRQNPASLERAVEQAGYQLIRPEKVEGAADKGPSKEEKLWHR
                     FVWSAAFTLPLLYIAMGPMLPWGGLPLPALLHQPLVYAISQVILLIPILYIGRSFFQK
                     GFKTLLQGHPNMDSLIAVGTGAALVQGLLMIAFLLMGKEVAMHGHHPELYFESAAVIL
                     TLITLGKYFEARAKGQTSEAIKKLMNLAPKTAQVLRNGQEIQVPIEEVVVGDQVVVRP
                     GQQIPVDGQVLEGQTRVDESMLTGESLPVKKALGDNVFGGTLNQQGAITMQATKVGRD
                     TTLAQIIRLVEEAQGSKAPIAKLADQVSAIFVPVVMGLALLSGLAWYFLGQESWIFSL
                     SIIIAVLVIACPCALGLATPTAIMVGTGKGAENGLLFKSGQAIETLQGVNTIVFDKTG
                     TITEGKPQVTDVHLLSTKNREQVLQLAASSEQFSEHPLAQALLQAAQTEKIDLLPATD
                     FQALSGRGLSVIIAEQTIYLGNERLMREQGIDVSKGRAVAEAFAQQAKTPVFLASQQE
                     VLAVIAIADKIKETSRQAVQALQTIGLEVVMLTGDNEKTAKAIAKEVGIEQVISQVLP
                     DDKANQVKHLQEQGKTVAMVGDGINDAPALAQAHVGLAIGSGTDIAIESADIVLMHSD
                     ILDVVKAVKLSQATMRTIKQNLFWAFAYNVIGIPVAMGLLHVFGGPLLNPMFAGAAMA
                     LSSVSVVLNALRLKTYKL"
     gene            18803..19288
                     /gene="tpx"
                     /locus_tag="AC1PNG_000260"
     CDS             18803..19288
                     /gene="tpx"
                     /locus_tag="AC1PNG_000260"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HIP6700799.1"
                     /translation="MTTFIGKPVTLVGPQLRVGDVAPDFVLMANDLSLKSLKDFGKQT
                     KVISVVPSIDTGICDTQTRRFNQELADRDNTVVITVSADLPFAQKRWCGAAGLEDAIT
                     LSDYYDHAFGKSYGVLMREWNLLARAVFVLNADNKITYVEYLDNVNEHPDYDAALEAV
                     K"
     gene            19383..19718
                     /locus_tag="AC1PNG_000261"
     CDS             19383..19718
                     /locus_tag="AC1PNG_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HIP6700800.1"
                     /translation="MEKTQIIQDVLALIQDLNRAFQADKVGTADEQRFQHNLTETTRI
                     LSEAESVTNTELIAIEKFYRSTSFLVGLGDLRLSESSRQAWRAFDRYYYETIREELKL
                     YGGSAIAQV"
     gene            19807..20508
                     /locus_tag="AC1PNG_000262"
     CDS             19807..20508
                     /locus_tag="AC1PNG_000262"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6700801.1"
                     /translation="MSYIQVKNSSKYYQMGDATIVANDGVSFEIEQGELVIILGSSGA
                     GKSTLLNILGGMDSNDEGEVWIDGVDIAKLSSHELTNYRRDDVGFVFQFYNLVANLTA
                     KENVELAAEIVKDALDAEEVLEQVGLGHRINNFPAQLSGGEQQRVAIARAVAKKPKIL
                     LCDEPTGALDYQTGKQVLQILQDMSRTQGTTVIIVTHNSSLAPIADRVIRMRDARVHS
                     VELHAEPQDIASLEY"
     gene            20520..23885
                     /locus_tag="AC1PNG_000263"
     CDS             20520..23885
                     /locus_tag="AC1PNG_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIP6700802.1"
                     /translation="MKKKIYWKDMRQSLLSSKGRFLSIFSLMMLGALALTGLKVTAPN
                     MEKTAQAYIASHRTMDLAVISGLGLSQADLDELETIEGATLEAGYFKDVVTNEGQTAI
                     RLFSAPQTLSTYKLVEGEMPSQKGQIALSASFKNRYKLDDTVQVTESDKDGKILTQTS
                     FTLVGFVASAEIWDNETMGMAASGDGQLGGYGLVSQDTFKSEVYTIARIRYDDLVDLP
                     YYSQAYQDRLDQHQEDVEKLLANNDEQRLAAIQSDGQTDISKGEEEIAQAQSQLEQAE
                     SELETGEEQLATGRREFARGRAKVVQTEAELRAAQAQLLKNKFELEESKKELDGRKAQ
                     LEQTASFLDGYQMELETSAQQLEEAKQELDSQNAQLSQTASQISTGRASWFQAQQELD
                     LEIANHLQEGQTLSDYPDLLARQEGLDVEKSRLDQMESAHEQANQAYLQGYDYYQTKQ
                     NEYNSNLAQYQTWNQEYQAGLARYQAGLSQYEQGIAAYNKGVEDYEWGLSQLESSNQL
                     LRQEEFRLEEADKELSQAQSQFSEKKATADQEISQAQTEIAQAKSDLSKLEKAPYQVY
                     TRSSLPGGDGYTTYSNATRSIAAVGNVFPVVLYLVAALVTFTTMARFVDEERTQSGLF
                     KALGYTNRQIMAKFILYGLTAGLVGTIVGIIAGNLLLSPLISNIITQTTVIGPAKLHF
                     YPLWTGLALLLSLASSVLPAYLVARRELTEKPAQLLLPKPPVTGSSIWLEKWPAIWSR
                     LSFTHKVTARNIFRYKLRMLMTIFGVAGTVALLFGGLGIRSSISGVVQRQFGELIHYD
                     MLVVENSRATEEELDKLTHFLQSDQVRQSLPVAFEQLNQTVEEDGQRKNLSISLYISD
                     RRDFGNQVSLESSTGQPIKLSDRGIVLTEKLAQIYGVSVGDKLSLTLEDKEVSVRVEA
                     VADMYAGHFIYMTDSYYEQVTGKQKTANAYLVQLKDSQLGHIQTLASQLLAMPAVRSL
                     VQNTSLIDMLTTIAGSLQTIMTILVILSILLGLVILYNLTIINMSERIRELSTIKVLG
                     FHNKEVTMYIYRETIVLSLIGMLVGLVGGIYLHKLLLAMIGSDSIRFNPSVGLEVYLI
                     PILAISGILAVLGWYVNHHLRKVDMLEALKSVD"
     gene            complement(24342..25514)
                     /locus_tag="AC1PNG_000264"
     CDS             complement(24342..25514)
                     /locus_tag="AC1PNG_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIP6700803.1"
                     /translation="MDVLYQSCAGIDVHQANIVVCILHGPLTSTRPKREMATFDTTTK
                     GLRACHDFLSQFHVEAVGMESTGVYWRPVWHALCDDFKLILAQPAHMKAIPGQKTDKK
                     DAHWIAKLTRIGLLPRSFVPDEAIQELRELTRQRKHYVESRNRETNRIHKILQSGGIK
                     LTTYIEDIMGLSGRNLLQLLVDGTPITPRIVHQSVYTSLKKKVPQLLEALDGYFSDHH
                     RFMLKQSLEIYDFYQKQIELLEERMNVYLSQYENHVEILDSIPGIDVITASVIISEVG
                     VDMSQFPTAGHLASWAGLCPGNNESAGKKRSTKIRHGNSYLKKCLCQAAFAIKKQKGS
                     PLADRFYQIQSRRGSQKATIALAHQLLKIAYILLKEQITYPEFLAQKKTTRDELVA"
     gene            complement(25770..26021)
                     /locus_tag="AC1PNG_000265"
     CDS             complement(25770..26021)
                     /locus_tag="AC1PNG_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700804.1"
                     /translation="MFKEFNKEFYRSFNFYLMILLLLGSIGEWLDKGPTLWLGLLLFA
                     SVGTARSAFWSKEWMLRANWQIYLCIAFLLVGAWVEFFV"
     gene            complement(26031..26285)
                     /locus_tag="AC1PNG_000266"
     CDS             complement(26031..26285)
                     /locus_tag="AC1PNG_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700805.1"
                     /translation="MSKKFNKAFYRTWEFYLSLACILQVISAAVNGPFWFIHFLKLGL
                     GLYAGYLAFFKSRSWIKKTWQIYLTIGLIASWTIISFFVL"
     gene            complement(26299..26430)
                     /locus_tag="AC1PNG_000267"
     CDS             complement(26299..26430)
                     /locus_tag="AC1PNG_000267"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700806.1"
                     /translation="MIILIHAIRTFFFKEEKELTRMGYIFYIGLIVVIIYLKFILGK"
BASE COUNT         7445 a         5319 c         6422 g         7404 t
ORIGIN      
        1 cctgtcaggc tttgccaggc gaacctctgt atagggaaga aggcggtatt atgcctttac
       61 tagtcaaggc tgcccaagaa gcaggggctg tcggtattcg ggccaatagt gtacgagata
      121 ttaaggagat aaaagaagta actactttac caattatcgg aattatcaag cgagactatc
      181 ctccacaaga gccctttatt acggctacga tgcgagaagt agacgagtta gcagctctag
      241 atattgaagt cattgctctt gattgtacca agcgtgagcg ttatgatgga ctcgatattg
      301 ttgatttcat taatcaaatc aaggaaaaat accccgaaca actttttatg gcagatattt
      361 caacctttga ggaaggatta actgcttatg aagcaggcat cgatttcatt gggacaacac
      421 tttcggggta tacatcctat agtaggcagg aggagggacc agatattgaa ttggttgacc
      481 gcttgtgccg agcaggtatc gatgtgatag cggaaggaaa aatacactat cctgaccaag
      541 taaaaattat tcatgattta ggtgtggcag gaattgttgt aggcggtgca atcacacgtc
      601 caaaggaaat cgcagagcgg tttattagtg ctttacataa ataataaata ggttatagaa
      661 aggaatggag tatgaatttt aagaagtttt ctcagctagg gcaggccttt atgctgccaa
      721 tctcaatttt gcctgttgct ggtctcttac tgggaattgg aggcgcctta tcaaatagta
      781 atgctgtagc acaattccct gtattggatc aagcgtggtt gcaagctgta ttcactatca
      841 tgagtacggc aggtagtgcg gtatttgcga atttagcatt gatttttgca attggtgttg
      901 ctgtaggttt ggcagatgct gataagggga ctgctggcct atctgctggg gttgcatact
      961 tggtttatac ggctactatt agtggatttt taactctttt tgcagataag ggagcaagca
     1021 ttgatactgg agttttgggc gctttagcga ttggtatcac tgttgcaact ttacacaata
     1081 aatatcgaaa aattgaatta ccagcctttc ttggtttctt cgggggctct cgctttattc
     1141 caattatttc atcatttgca gcaattgcga ttggctccat tttttatata atttggccac
     1201 cgattcagaa tttgttagtt gattttggta aggcaatagc agagatggga agttttggaa
     1261 ctttcttata tggttttgga ttacgtttga caggtgcatt tggcttgcac cataccatct
     1321 atccaatgtt ttggtatact tcattaggcg gaagtgaagt ggtagcaggt gagacaatcc
     1381 aaggtgcgca aaatattttc tttgctcaat tagcagatgc gaatcacaca ggcttattta
     1441 cttatggaac tcgatttttt gctggtcgat ttgcaacgat gatttttggt ctaccagcat
     1501 cttgtttggc tatgtatcac gcaattccta aggaaaatcg taagaaggtt ggcagttttt
     1561 attcaagtag tgcattaact tccctattaa caggaattac cgagccaatc gagttttctt
     1621 tcttgtttgt tgctccatgg ctatatgttg ttcacgcatt tttagatgga ctatcattct
     1681 tagtcgcaga tctcctgcaa attcgtattg ggaactcatt ttctggtggg cttattgatt
     1741 acttgatttt tggtgttttc caaggaaatg acaaaaccaa ttggatttac gtgattcctg
     1801 ttggcttgct ttgggcagca gtttattatc tagttttcaa atttttagta accaaatttc
     1861 acgtgctagt tcctggtatg gaaagtagtg accaagcatc acattctgaa aaagttttat
     1921 caaacggttc tagtcttcgt gataatgcaa ttgctatcat cgatgctctt ggtggtgcag
     1981 ataatattga gaatgtaact gcctgtgcaa ctcgattaag agtatcgctc agaaatagtg
     2041 gtcttgtgga taagggagtt attcagtcta taggtgcaac ggctgttttg gatgtaaaag
     2101 gaggaataca ggctattttc ggaggtaagg ctattctgta tagccaagaa atcaatcaaa
     2161 ttcttgggct tgaagaatag agttatattt aagcgaatta aaagcattca aactgtacct
     2221 atcgggtatt tttgaatgct ttttgtttta ttaagattgt tgtgaaatag aaagacggat
     2281 gaattattct ctaatggttt gcttgaaaat attctctttt ttctccttgt caatagctaa
     2341 aaaataggcg tagataatat ctagcatgat gagcaggggg atttggggtg agatacgatt
     2401 gccgtaatta aggtggcgga cagaggcgac tggaatgatt tggtcaaaag tcggatgggg
     2461 aaaactctta gttgttaaca gggcggtagg aatccccttt tgagcagcca gctggagatg
     2521 ttccatgacc tcttctgtct gaccagacag agaaagaccg ataaccaggc aactatcatc
     2581 cagaatattg gtagtccaga tgagattgtt ttcatcagta attgcatcac agactacacc
     2641 cagtcgcata aagcgggatt tgatttccat tgcaaccaaa ccagaactgc cgattccgta
     2701 aaaatagaca cgcttgctac tgtcaatcag tttggcaatt tcttccaatt tttcctcatc
     2761 taccagttta ttggtagcag ttaggatttc ctcatagtct accaagactt tttttgttag
     2821 gctccgctgt aatttttcaa aatgtttatc caagtattgt tggttattct gaaaggcaaa
     2881 gacaaattct cgataaccgg aaaatccaca ttttttggca aaacgggtaa gagcagatgg
     2941 agaaatgtgg agttgctgta tagtgttttc ttggctaaga tcggcgctag atgggtctaa
     3001 ctgagtaaaa taactggcaa tctgcttttc aagtctagtc atctgatcca gatgagactc
     3061 aatgatggca gtgatgtgtt tcggtgtttg taacattttc cattcctctg atagcttctg
     3121 aggacattat accactaaaa ccccctcatt gataggtttt atgatataat ggaaacgtaa
     3181 aaggaatgta agattatgta tattgaaatg attgatgaaa cagggcaggt ttctgctcaa
     3241 atgcaggagc agattacgga attgctccag tttgctgccg aaaaaattgg caagcagaat
     3301 aaggaaatgg ctgtgacctt tgtggacaat aagcgtgtcc atgaggttaa cttggagtat
     3361 cgaggaattg accgtccgac agatgttgtc agcctagaat ataagccaga gtcggagatt
     3421 gtttttgatg aggaagactt actggacaat cctgaattgg ctgaaatgat ggaagatttc
     3481 gatgcctata ttggtgaatt gtatatttct atcgataagg cgcgtgagca agctgaagat
     3541 tatggtcata gctacgagcg cgaaatgggc tttttagctg tacacggttt tctgcatatc
     3601 aatggctatg atcattatac gccagaagaa gaagcagaaa tgtttggttt acaagaagaa
     3661 attttgacgg cctatggact tacaagagaa taattcaaaa aatcgctgga aaaaccgaga
     3721 gctgattgcc agtctagaat ttgcagtaac gggtttgttg acggctttca aggaagaacg
     3781 caatatgaag aagcaccttg cttctgctat tcttgtggtg cttgcgggac ttattttcca
     3841 agtatctgtg acagagtggc tcttcctctt gctcagtatt agtctggtta tcgcttttga
     3901 aattgtgaat tcggctattg aaaatgtggt ggatttggct tcagactatc atttttctat
     3961 gttggcgaaa aatgccaagg acatggcagc aggggcagtc ttgtttgtgt caggatttgc
     4021 cttgctaaca ggattgatta tctttgtgcc gaaaatttgg gactttattt tttagaatat
     4081 aaggagaata catgacattt aagtcaggtt ttgtggcgat tttaggtcgt ccaaacgttg
     4141 ggaaatcaac ctttctcaac tatgtaatgg ggcaaaaaat tgccatcatg agcgacaagg
     4201 ctcaaacaac ccgtaacaag attatgggca tttatacgac agaagaagaa cagattgtct
     4261 tcattgacac accgggaatt cacaagccta agacagcttt gggcgatttt atggtggagt
     4321 ctgcttatag cacccttcgt gaagtggaca cggtcctctt tatggtgcca gccgatgaaa
     4381 aacgaggcaa gggtgatgac atgattatgg aacggctcaa gcaggccaag gttccagtta
     4441 ttctcgttgt taacaagata gacaaggttc atccagacca gcttttggaa cagattgatg
     4501 atttccgtca acagatggac ttcaaggaaa ttgtgccaat ttctgctacc caagggaaca
     4561 atgtcaaccg acttatggaa attctcaagg aaaacctgga cgaaggcttc cagtatttcc
     4621 cagcggacca aatcactgac cacccagaac gtttcttggt atctgagatg attcgggaga
     4681 aggttctaca cctgactcgt gaggaaattc cgcactctgt ggccgttgtc atcgagtcca
     4741 tgaaacgtga tgaatttacc gacaaggtcc acattcgtgc tacgattatg gtagagcgtg
     4801 atagccagaa aggcatcatc atcggtaagc aaggagccat gctcaagaaa atcggctcca
     4861 tggctcgtcg tgatattgaa ctcatgctag gtgacaaggt cttcctcgaa acctgggtca
     4921 aggtcaagaa aaactggcga gacaaaaaac tcgacctcgc cgactttggt tataatgaga
     4981 aagagtatta aaaacgtccg ggggagtgaa acagtctagg aatagactgt ttcagctggt
     5041 cacctagaaa tgtgaaagtg accatttgaa aacccgagca tagaaattgg aaggcgagat
     5101 agcctgggga taggctgtct aagtcaatca cttagaaata gttagcagaa aagccgaaag
     5161 gcttttactg cgttatggag aaatcttttt agtaaacaag aaaggaaagt cgattaggtg
     5221 ttgtcttgaa cacgcctacg actctttgaa aaaagataac cgtgacctag gtgccgaagc
     5281 accatcggtc aggtttctat ttgctttgta attttgttag aaaggacgag tgtttgcgta
     5341 cttgaacccg agctaaaagc tcggaaaata gatagagctc cttatgtgca agtcacatag
     5401 cgtcgctctc ctaattttac tatatttaga aaggaaagcc gattaggtgt tgtaattgaa
     5461 cacgcctagg cctccttgaa aatcacaacc gtgacctagg tgccgaagca ccgtcggtca
     5521 ggtttctatt tgctttgtaa ttttgttaga aaggacgagt gtttgcgtac ttgaacccga
     5581 gctaaaagct cggaaaatag atagagctcc ttgtgtgcaa gtcacacagc gtcgctctcc
     5641 taattttcag tcgcttttta cgctcttggt atcgtacatt gcccgaattg cctgaggttg
     5701 agacggttcg tcgtggtttg aatcgtttgg tcaagggaaa aattatttcc aaggtggagg
     5761 tgacctatgc tcccatgatt aagacaggtg tggatgcctt ctgtcaggac ttgattggtc
     5821 aggagatttt agatgtggac cgtcgtggca agtatctctt gatttacctg acagaccatg
     5881 tcctcatttc tcatttgcgg atggaaggta agtacaattt ctttcctgac caggtgcctg
     5941 ccaacaaaca tttccatgcc ttttttactt tttcagatgg ctcgaccttg gtctatcaag
     6001 atgtccgcaa gtttggcaca atggaactgc tgggcaaggc agatgtggat gcctatttta
     6061 tcagtcgaaa aattggtcct gagccgacgg aggaagattt tgacttagaa gaatttgcca
     6121 aaaagttagc taagtcgaga aaacccatta agtcgcatct tttggaccag tctttggtgg
     6181 ctggtttggg caatatttat gtcgatgagg tcttgttcaa ggccaaagtt catcctgcac
     6241 aaacgagtaa tcagctatca gcagagcagg tggcagatct ccgccaagct accatagaag
     6301 ttctacaact gggaattgaa aaaggtggtt caaccattcg gacttacaaa aatgccttgg
     6361 gcatggatgg gactatgcag gattatttac aagtttacgg aaagacaggg caggcctgtc
     6421 cccgttgcca gacagagatt gtcaaaatcc agctgggcgg acgggggaca catttctgtc
     6481 ccaagtgtca ggtgaaacat ggctaaaatt atcggtttaa caggcggaat cgcctctgga
     6541 aaatcaatgg tcacagcttt tttgcgagaa cagggctatt cagtcatcga tgcggatgcg
     6601 gttgttcacg aattacaggc caaaggtggc aagctctatc aggttttggt gaaagagttt
     6661 ggtcaagaca tcttatcaga tgatggaaat ttggataggg ctaagctggg ccaggctgtt
     6721 tttgcggata gcaagttgcg ggctcgcctg tctgacctgc aggaccaaat tatcaggcag
     6781 gagctgttag acagaaggga tgccctcaag cagacggagg atgtgatttt tatggatatt
     6841 cctttgctct acgaggcgga ttatagcggg gaagtaaatg aagtctggct agtttatgtc
     6901 gatagagcgc agcagttgga acggcttatg aaacgcaatg gcttagctgt tcaagatgca
     6961 gaaaatcgta tggctgctca gctttcttta gaggaaaaac ggtcgcaagc ccaggtggtg
     7021 attgacaata gcggagcggt tgaggcgacc ttagtgcagg ttgctcggct tttggaggaa
     7081 ttaaaggatg gacgacggta gtagttattg gaagcagaat ttgaaggtgg cttggttggg
     7141 aaatttccta acagggacga gttttacctt ggttatgcct tttatctcgg tttttgtgga
     7201 agagttggga gtgggccctg gacaggtaga atattatgcg gggcttgcgg tatctgttaa
     7261 tgcccttgca gcagccttga tggcgcctat ttgggggagt ttggctgacc gctatgggcg
     7321 caagcctatg atggttcggg cggcttttgc tatgatcttt accatgggtg gaatggcttt
     7381 tgttcccaat gttttctggc ttcttgttct tcgtgtactt aacggtgtct ttacgggtta
     7441 tattccaaat gcgacagctc tgattgccag tcaggttcca aaggataaga cggggtatgc
     7501 tctgggtacc ttatcaacag gtgcggtagc gggtaatttg attggtccga ccctaggtgg
     7561 tatcttagca gagatgtttg gcgtacatat ggtatttttg ctggtgggac ttctttatgc
     7621 tatcgttgtg ttactgaccg ttttctatat tcgagaggat tttgttccaa tcaaaaaagg
     7681 cgaagaaatg tcggttaaag aggtatttga gcaggtgaaa gatcggcaga tgctggttgg
     7741 gctttttgtg acatctatga ttattattgc agctgctcag gccgtcgttc ctatattgac
     7801 cctctatgtg cggcatttgg gacagacaga taatctgtta tttgttgctg gttttatcat
     7861 ttccttgcct ggtatggcat cgctagtgac atcaggttat ttagggaaaa ttggtgatca
     7921 cattggtaat catcgacttc tattaattgc tttgacttac agtctgttga ttaatgtttt
     7981 ctgtgttttt gcagaaaatc ctttccagct tggtttgttg cgattcatgt atggttttgg
     8041 gacaggagca ttgttgcctt cggtcaattc tcttttgacc aagttgaccc ccaaagaagg
     8101 gatttcaagg atattttcct acaaccaact ctttaataat ctaggttctg ttgttggtcc
     8161 catgatgggg tctgcggtag ctgctcacat gggttatgac tgggttttct atctgtcgag
     8221 cggacttgtc ctctttaacc ttatctggtc tttgaccaat tttagaaact atttgaaagt
     8281 aagggatgtt tagtgagagt taaaatcaat ttacaatgtt cagaatgtgg tagtaagaac
     8341 tacttgacta gcaaaaacgc caagactcat ccagataaga ttgaagtcct taaatactgc
     8401 ccaaaagaga gaaaagtaac cctccacctt gaaactaagt aggttttatg gtataatgtc
     8461 ataagttatt gtagaaagtg aaaaaaatat gtatcaagca ttattagcaa ttctgttgat
     8521 tttatctgtt attttgattg tagtgatttt tattcaaccg gctaaaaacc agtctagcaa
     8581 cgtctttgac tcttctagtg gagccttatt tgaacgtaca aaggcccgtg gttttgaagc
     8641 ggtgatgcaa cgcattacag ctattcttgt ttttctttgg atgctggatg cgctggcgct
     8701 tgttatcatt tcaagtaagt agataaatca gactgggact tgttcttggt ctattttttt
     8761 gggagtgggc cagaactcga caagtataaa gagttcgtct gcctaccccg cacaagttga
     8821 ataggtatgg ttcggagcgt tattcgcgaa ctattgagat ttgctttgca aatcctatct
     8881 cccacctcca acaatcattt gattgttgga gcaatcaacc actgcgtctg gatataacgc
     8941 aaaatacagt cttttagttt gcttttagta ctaggcaacg agctgcagac tgtactgaag
     9001 tacgacaaag cgagttcaaa taatgatgaa attatttgaa gttggaaata gggaaacgga
     9061 gtttcctcgt acgtcgcagt aataaaagat aaactaaagg actgaaagag aaatagtgct
     9121 aggtttttat cctgcactct ggtaaataca acatgaaaaa cgatattatt aactatatta
     9181 aagaagttgg tcctgtgacc atggaccagc tggcagatca gtttggagcc agttctgcta
     9241 agagctttac agacttggtc aagctggttt ctagcatgga aggcagtcgt caacttgtct
     9301 ttgacaatgc tgggcggatt actcttcctg ctccaaaaat ctccaagaac aaggtgacct
     9361 tacagggtat tttccgtgcc cacaagtcgg gttttggctt tgtgaccatt gacgaggagg
     9421 aagatgatct ctttgtcagc cgtgacgatg ttaactttgc tatcgaaggt gacagagttg
     9481 aaattgcaat caaaaaggtt gcagatcgtc tcaagggaac tgccgcggaa gcggaagtca
     9541 ttgatatttt ggaacatagc ctgaaaacag cggtgggctt gattgtcttt gacgaagata
     9601 agccagaata tgcaggctac atcaagtcta aaaaccagaa aattgctcag aagatttaca
     9661 tcaagaagtc acctctggta ttgaccggaa cggaaatcct aaaagttgat atcgaagctt
     9721 atccaaacaa gaaacgggac cactttgttg cgaccattcg ggacgtggtg ggccacaagg
     9781 acgatgtggg catcgatgtc ttggaagttt tggagtccat ggatatcgtg tcggagtttc
     9841 cagatgaagt cttggcagag gccaatcggg taccagaaag tccgtctgaa caggactttg
     9901 caggacgttt ggacctgcga gatgaaatta tcttcaccat agacggtgcg gatgccaagg
     9961 acttggacga tgcggtccat atcaagcagc ttaagaacgg caatctggag ctaggtgtcc
    10021 atatcgctga tgtcagctac tatgtgacgg aaggctctgc cttggatcaa gaggccatca
    10081 agcgtggaac gtctgtctat gtgaccgacc gtgtggtgcc aatgttgcca gaacgtttgt
    10141 caaacggcat ctgctctctt aatccaaacg tggaccgtct aacccagtcg gctattatgg
    10201 aaattgaccg caagggcaag gttgtcaagc actggattgg ccaaacggtc atcaaaacca
    10261 acttccgcat gacctattct gatgtcaatg acatgattgc gggaaatcag gaaaagctgg
    10321 acaaatacaa gacaatcgtg ccaagtgttg agctcatggt caaactccat gaaaccctgg
    10381 aaaccatgcg ctacaagcgt ggggctctca actttgatac agctgaagct aaaatcatcg
    10441 tcaacaagga tggtctgcca gtcgatatcc agctacgtca gcgaggcatt gctgagcgga
    10501 tgattgagtc cttcatgctg gttgccaatg agtgcgttgc tgagcatttt gccaaactgg
    10561 acctgccttt catctatcgg atccacgagg agcctaagtc agacaagctg caaaagttca
    10621 tcgactatgc gaccagcttt ggtctgacgg tctatggtac ggccagctct atcagccagg
    10681 acgcccttca ggacctcatg gagcgggtca aggatgaacc ctatgcggat gtcctcaaca
    10741 tgatgttgct gcgttctatg cagcaggcac gctactcgga gcacaaccac ggccattacg
    10801 gtctgggtgc cgagttctac acccacttta ccagccctat ccgtcgttat cccgacttgc
    10861 tggttcaccg catggtgcgg gactatggtc ataatcctgc ggaaaaagca gagcattttg
    10921 agcaggttat acctgagctt gccaagtctt cgtccagttt agaacgccgt gccattgagg
    10981 cagagcggga agtcgaagcc atgaagaagg cagagttcat gcaagaattt gttgggcaag
    11041 agtttgatgg tgtcgtatct agcgtagtca agtttggtct ttttgtagag ttaccaaaca
    11101 cggttgaagg cctgattcat atcaccaacc tcaacgaata ctatcagttc cacgagcgaa
    11161 ctctgacctt gcagggtgag aaatcaggtc gggtcttccg tgtcggtcag ccaattcgta
    11221 tcaagctaac gcgggcggac aagatgacag gggaaatcga ttttgcccac gtgccgtccg
    11281 agctggatat tgtcgaaaaa gctctgaaag ccaaacggag aactgctagt cactctaacc
    11341 gtgaccgtga tgatcggtca gggcgaggac ggggcaagca ccacaaacaa gaagctgctg
    11401 gtcgcgacag cggacgtcac aagtctggaa aagaccacaa gtcaaaaagt ggcaaaaaag
    11461 acaagaaaaa gaaaccattt tacaaagaag tggctaaaaa gaacaagaag aaaaggagat
    11521 aggcatggcc aagggtgagg gcaatgtgat tgcgcaaaat aaaaaagccc gccacgacta
    11581 taccattgtc gatacagtgg aggcggggat ggtcctgacg gggacggaga ttaagtctat
    11641 tcgtgcgggg cggatcaacc ttaaggacgg ctttgcccag atcaagcagg gcgaggcttg
    11701 gttggtcaat gtccatatcg ctccctacga tgagggcaat atctggaatc aggagccaac
    11761 gcggacccgc aagctcctct tgcggaagaa gcagattgag tctctgggca atgaggtcaa
    11821 gggaacaggg atgactctgg tgcccctcaa ggtctatatc aaggatggct ttgccaaggt
    11881 tttgattggg cttgccaaag ggaagcacga ctacgacaag cgggagtcaa ttaaacgccg
    11941 tgagcaagac cgtgatatca aacggaccat gaaggctatc aatagtcgat agaggctggg
    12001 agaaatcctg gtcttttttg ttatcggaaa acaaataccc cagacagttt cgagactatc
    12061 tggggcttat tatttatctt aatctaccgt aatcatagct ttaatcgttt tccggttggc
    12121 catgtctttg taggcttggt cgatattgtc caggctgtaa gtttgggtga aaactcgacc
    12181 tgggttgatg tcgcctttga gaacggcatc gagcaaaatt tccttatcat aggttgtgac
    12241 agaagcagag ccaccaccga taatgatatt ttgagcaaag gttgagccaa gggcgtggtt
    12301 gttgtagtgt ggtacaccga caaaaccgat acgaccacca ttgtgaagca cgccgagggc
    12361 ttggtcaata gaggcttctg taccaacaca ttcgagggca gcatcagcgc cacctccaag
    12421 aatttcacgg actttggcaa ttccttcctc accacgttct gctacgatgg cagtagcccc
    12481 tgattccaaa gccatttgtt ggcggtctgt atggcggctc atcatgacaa tctgagaagc
    12541 tccgcgcatt tttgcagcaa ttacagcaca ttgacccact gcaccatcac cgatgacaac
    12601 aaccttgtca ccaggtttga catctgctac acggtctgcg tggtagccag ttggcataac
    12661 atctgccaga gtcaagagag atttgatcat gccttctgag tagtcacttg gctgaccagg
    12721 aattttgacc aaggcccagt tgccgtagtg gaaacgaatg tattgagact ggtagttgtt
    12781 gccccagttg gtgtagcctg gatggttgtc acaagtaccg tcaaagccag cacgacaggc
    12841 atcacagtgt ccgcaaccat gtgtaaatgg aacgataacg aagtcaccag gaacaagtgt
    12901 tgtgatgtct tcgcctacct cttcaacgat accgatggct tcatgaccag agtttgtgtc
    12961 accgtggtca tggtgaatgt cttctccgta gccccaaagg tccgatccac agacacaggc
    13021 ccgaacgacc ttgataatga catcatcttt ggcttgaagg cttggttttt cgatttctgc
    13081 aacggtcatc ttaccggatt tttcataaat agctgttttc atattcatca cacatttact
    13141 tgtcagtaaa tatcctttct gattaatatg gaatattata caccttggag ttcactccaa
    13201 gtcaagaaaa aaatgggttt gaattcatat ttcctttgtg atagactaag tgtatcggaa
    13261 agaaaaggag agataatgac tgttttagtt ccttacaaac gcatgcccat ttggaatcag
    13321 gatacggttc cccagcattt tttgaccaag cacaatacca aggtgggtac ttgggccaaa
    13381 atcaaggtct tgagaggtca acttaaattt gagccgattt cagatgataa tgagattttg
    13441 ggagaatatg tctatggtcc gacagccgat attccctttg tcgagccaca ggcttggcat
    13501 cgggtgactt tgctgacaga ggatacggag tttttcttgg aatttttctg tcaaccagaa
    13561 gactattttg ccaaaaagta tgactatact cggacacatt cggaagtctt ggaggccttg
    13621 aagagaattg agccttgtaa ggtcttggac ctgggctgtg gtcatggtag gaacagtctc
    13681 tacttggctc agcagggatt tgaggtgacg gcggtcgata aggatgtgcc aagtattcgg
    13741 acactcttgc aggtcaagga agtggaggac ttggacttgc aggctgggac ctatgacatc
    13801 aattcagcca gtctggagca ggactacgac tggattatct cgactgtggt cttcatgttt
    13861 ttggagcggg atagggtacc agccattatc cgcaatatgc aggagcagac acgacctggt
    13921 ggctataact tgattgtggc ggccatggat acggaagatg cgccctgtcc tatgaatttc
    13981 tcctttactt ttggagtagg tgagttgaag gaatactacc gtgactggga actggtcaaa
    14041 tacaatgaag atttcggtca acttcacaag cgggatgaaa atggccattt cctcaagatg
    14101 cggtttgcga ccatgctggc taggaaaaaa ggataaatat ttttttgaaa gcacttgcaa
    14161 ggcaggtgct tttgcgttac aatgtaggtg caaatgggta aatcatatta tactccacga
    14221 aaatcacctt ctggttggat aagagacttg tgtaaaatag tctctgagtt tatatctctg
    14281 gagggcgcct acttctgatt ttcacagagt atcaggaaag gagaagtcta tgaaaaagac
    14341 ttttgttcgt atagccacat ttctaatgtt ggtttgccta ttccctgttc aacttgttca
    14401 agcctgttca ggctttatta ttgggaaagg gttgacaacg gatggttcta tcctttatgg
    14461 tagaacggaa gattatccct atccaccaga taatggagcg cacaataaaa attatattgt
    14521 ggttccagca gcagcctatg ccaaggggga tatgttggtg gatgaatcat tcggtttcac
    14581 agcaccacac ttggcaaatg agttcaagta cacctctacg cctgatgcgg cacgtgggga
    14641 tgggtcaaac ggtaattttg gtgcgcatgg atttaatgaa aaaggtgtgt ccatgactgc
    14701 tactgttaca gctattccaa ataaaaaagt tttagctgtt gacccattgg taactgcggg
    14761 gggactaggt gaggccattt tgattgacta tgttcttcct agagtcacca gtgcgcgtga
    14821 aggtatcgaa ttaattgcga agacaattga tgaaaaaggt tcggcagaag ggaatatcat
    14881 tgttattgct gataagaatg aagtttggta catggaaatt ttgtcaggcc accaatacgt
    14941 agccatcaag ttccctgaag acaagtatgc aatttttgca aatacttact atctaggtca
    15001 tgttgatttt accgatacag aaaatgtgat tgcttctgca aaggttgaag aagtagccaa
    15061 acaggctgaa aattatatga tggttgatgg gaagttccat attgccaaat catatggacc
    15121 tgagaattat gctgatggtg atcgttctcg gacatacgct ggtataaaat tgttagatcc
    15181 agcatcgtct gtaacctatg aagatgctgt gtatgatttg ctacgtcagc caacagaccc
    15241 aagtcgtcgc tttagtttac aagatacctt tgccttacaa cgtaaccgtt ttgaacatct
    15301 tccagaattt cgtccagacg atgaagcagg taaggtaaaa caaggggata atggtgcgaa
    15361 tgaccaagcg gcagatgcga cttataaata cgctcttggt aatgaaaacg tcatcgatgc
    15421 ccatgtttat cagattaata gtagcttacc atcagccttc ggtggcacag tatggttggg
    15481 cttggctcag acaaggaata caccgtatgt tccattttat ggtattgtga ctgatactta
    15541 tgaagccttt aaaaatcgct cagcttctta tgataccaat tcatggtact ggacggtaca
    15601 aaatattgat aagatggcca tctcacaccc agagttattt ggtacatcaa ttcaagaaaa
    15661 atggattgca cttgaaaaag aatggattgc ttcacaggca gctcttgatg ctcaatatgc
    15721 tggactttca gaagatgcag ccgtagctct ggctccaact gtaacagagg caaccttggc
    15781 tcgttcagca gagatttttg cccaactaaa agctgttgaa gctgaaatga tggcaaaaat
    15841 tgaagcagca acaactccgt catcaagtag tactgaacca tcgaccagca ctgagccatc
    15901 atcaagtggt accgaaactt caaccagtac aagtcagtct acaagtgatt ctaacacagg
    15961 aggagctacg gatacttcct catcaagcgg aacagtagtg ccttctgata agaaagtaac
    16021 acctaccaat aaaaaaggca aatcaaatct tcctagtaca ggtgagcaag tcagcatctt
    16081 gttggttgcg ctaggtgttg caggaatttt aacagcaatt tttcttcatc ggaaaaaatc
    16141 cagtaaagaa taaatgaata tgtaaaagta ggttggattg atttcaatct acttttttga
    16201 aacaattttg tttacaaatg tagtcatatg ttgtataatt tgtttacaga tgtagtcgaa
    16261 agaggagtaa cctatgaaac aagcaagcta tcccattcaa ggtatgacct gcgcttcctg
    16321 tgctctgacg gttgagaaag cggttggtaa actaataggg attgaagaag ccagtgtcaa
    16381 cctagcgact gaaaaattga gtgttagcta tgatgaaaag cttttgggac tagaggatat
    16441 tcgtcaggca gttgaaaagg cgggttatca gttagtcgat aacttggtga ccgagtctta
    16501 tgacatttca ggcatgacct gtgcttcctg tgctctgacg gttgaaaaag ctctgggcaa
    16561 actagagggt gtagaagaag tctctgtcaa tctggcaaca gaaaaggcga ctattcgtta
    16621 tagccgtcat aggcaaaatc cagctagtct tgaaagagca gttgagcagg caggttatca
    16681 gttgattaga ccagaaaaag tagaaggggc tgcggacaag ggaccgagca aggaagaaaa
    16741 gctgtggcat cgatttgtct ggtcagcagc atttactctt ccactgctct atattgctat
    16801 gggtcctatg ttgccttggg gagggcttcc attaccagct ttgctccacc aaccgctagt
    16861 ctatgcgatc agccaagtca tcttgctcat ccctatcctc tatatcggac gcagtttttt
    16921 ccaaaaaggc tttaaaactc tcttgcaagg ccatccaaat atggattcct tgattgctgt
    16981 tgggacaggg gcagccttgg ttcaaggtct tttgatgatt gcgtttcttc tgatggggaa
    17041 agaagtggcc atgcacggtc accatccaga actgtatttt gaatcagccg ccgttatctt
    17101 gaccttgatt accctaggaa aatattttga agctagagct aaaggtcaga cttctgaggc
    17161 cattaagaaa ttgatgaatc tggctcccaa gacggcccag gtcttgcgaa atggtcagga
    17221 aatacaagtg ccgattgaag aagtggtggt cggtgaccag gtcgttgttc gtccaggtca
    17281 gcagattcct gttgatggtc aggttctcga aggccagaca cgtgtggatg agtctatgct
    17341 gacgggtgag agcctaccag tgaagaaagc tctaggcgat aatgtttttg gcggcaccct
    17401 taaccagcaa ggcgccatta ccatgcaggc taccaaggtt ggtcgtgata cgactctggc
    17461 ccagattatt cgcttggtag aagaggctca aggttctaag gctcccatcg ccaaattggc
    17521 tgaccaagta tctgccatat ttgtaccagt cgtcatgggc ttggctctct tgtccggtct
    17581 ggcctggtac ttcttgggtc aagaatcatg gattttttca ttaagcatta tcatcgccgt
    17641 cttggtcatc gcctgccctt gtgctctggg cttagcaacg ccgactgcca tcatggttgg
    17701 tactggaaaa ggtgcagaaa atggccttct gttcaagtct ggtcaagcta ttgaaaccct
    17761 acaaggtgtg aacaccattg tcttcgacaa gactgggacc attactgagg gcaaacccca
    17821 ggtgacagac gtccacctct tgtctactaa aaatcgtgag caggtcctcc agctggccgc
    17881 aagtagcgag caattttccg agcatccctt ggctcaggct ctcctgcaag cagctcagac
    17941 agaaaagatt gatctcttgc ccgccacaga ttttcaggca ctttctggtc gtggtctttc
    18001 ggtgataata gcagagcaga ccatctatct gggaaatgaa cggttgatgc gggaacaggg
    18061 aattgatgtt tccaaaggac gtgcagttgc ggaggcattt gcccagcaag ccaagacgcc
    18121 tgttttccta gccagccagc aagaagtact agcagtcatt gccattgctg ataaaataaa
    18181 agagaccagc cgtcaggctg tccaggctct gcaaaccata ggactagagg ttgtcatgct
    18241 gacaggagat aatgaaaaaa ctgccaaagc cattgccaag gaagtcggca ttgaacaggt
    18301 gatcagtcag gtcctaccag atgacaaggc taaccaagtc aagcacttac aagaacaggg
    18361 caagacagtc gctatggtgg gggatggcat caacgatgcc ccagctctag ctcaagccca
    18421 tgtgggtcta gccattggtt cagggactga tattgccatt gagtctgccg acattgtcct
    18481 catgcatagt gatattttgg atgtggttaa ggctgtcaaa ctcagccagg caaccatgcg
    18541 gaccatcaaa caaaatctct tctgggcctt tgcttacaat gtgatcggta ttccagttgc
    18601 tatgggtctg ttacatgtat ttggtggacc tctgctcaat cctatgtttg caggtgcagc
    18661 catggccctc agctcagtgt cagtcgtctt gaacgctctg cgattgaaaa cctacaaact
    18721 atagcaaaaa taccagtcaa aaaactggta tttttttaca taaacgctta caatgtatat
    18781 attaagtgca agggagatgt ttatgacaac ctttattgga aaaccggtga ccttggtagg
    18841 tccgcagtta cgagtgggag atgtagcccc tgattttgtc ctcatggcca atgacttgag
    18901 cttgaaaagc ctgaaagact ttggcaagca gactaaggtc atcagtgttg tgccttctat
    18961 tgatacaggg atttgcgata cccaaactcg tcgcttcaat caagaattgg ctgaccgtga
    19021 caatacggtt gttattacgg tttcagcaga cctccccttt gcccaaaaac gctggtgtgg
    19081 cgctgcaggt ttggaagatg ccatcaccct gtctgattac tatgaccatg cctttggtaa
    19141 atcctacggt gtgctcatgc gggagtggaa tttgctggcg cgtgcggtct ttgtcctcaa
    19201 tgccgacaat aaaatcacct acgtagaata cttggataat gtcaacgagc atccagacta
    19261 tgatgcggct ttggaggctg ttaaataaag atataacggt gccggaacag gcatcgtttt
    19321 ttgatggaat atttttgtct aatttgatat actaaattta tcaaaaacaa ggagggtctt
    19381 ttatggaaaa gacacagatt attcaagatg ttttggccct cattcaagac ttgaatcggg
    19441 cctttcaagc agataaagta ggaacagctg atgaacaacg gttccaacat aatctgacag
    19501 agacgacacg catattgtcg gaggccgagt ctgttaccaa tacagaactg attgcgattg
    19561 agaaatttta tcgttcgacc agttttttgg ttggactggg cgacctgcga ttgagtgaat
    19621 ccagcaggca ggcttggcgg gcatttgacc gatattacta cgaaacgatc agagaagaac
    19681 tcaaactcta tggcggctct gctattgccc aagtgtaaaa gatgaaggag agggtgaaaa
    19741 ccctcttttg tgctataata agtagtaaga atacagttgt taggaagaga agaagcgagg
    19801 tgtatcatgt cctatatcca agtaaagaat tcgtcaaaat actaccaaat gggggatgcg
    19861 accattgtgg ccaatgatgg tgtgtccttt gagattgagc agggtgagct ggtcatcatt
    19921 ctgggttcat ctggtgcggg caaatccact ctgctcaata tcttaggtgg catggatagc
    19981 aacgatgagg gtgaggtttg gattgacggt gtggatattg ccaagctttc aagtcatgaa
    20041 ttgaccaatt atcgtagaga tgatgtaggt tttgtatttc aattttataa cttggttgcc
    20101 aatctgaccg ccaaggaaaa tgtggaactg gcagcagaaa tcgtcaagga tgccttggat
    20161 gccgaggaag tcttggaaca ggtcggactc ggtcaccgaa tcaataattt ccctgctcag
    20221 ctttcaggcg gtgaacaaca acgtgtggcc attgcccgtg cggttgcaaa aaagcctaaa
    20281 attttgcttt gcgatgaacc aactggcgct ctggactacc aaacaggtaa gcaggtctta
    20341 caaatcctac aagatatgtc ccgcacacaa gggacaacgg tgattatcgt gacccacaat
    20401 agctctctgg ctcctattgc agatagggtc attcggatgc gggacgcccg tgtgcactct
    20461 gttgagttac acgcagaacc acaggatatt gcaagcctag aatattagtg ggtgaaacta
    20521 tgaaaaagaa aatctactgg aaggatatga gacaatctct gctttcttct aaggggcgtt
    20581 ttttgtccat atttagcctg atgatgctag gagctctagc tttgacaggt ctgaaagtaa
    20641 cagctcccaa tatggaaaag acggcccaag cctatatagc cagtcatcga acgatggatt
    20701 tggcagtaat ttcaggtctg ggacttagtc aagctgacct tgacgaatta gaaacgatag
    20761 aaggtgctac tcttgaagcg ggctacttta aagatgtggt tactaatgaa ggtcagactg
    20821 caattcggct gttttcagct ccccagaccc tatcgaccta taagctagta gaaggggaaa
    20881 tgcccagtca aaaggggcaa atagccctat cagcttcttt caaaaatcgt tacaaattgg
    20941 acgatactgt tcaagtgacg gagtctgata aggatggaaa aatcttgaca cagacaagtt
    21001 ttactcttgt gggctttgtc gcatcagcag aaatttggga caatgaaacc atgggcatgg
    21061 ctgcaagtgg tgatgggcag ttaggtggct atggccttgt tagtcaggat accttcaaat
    21121 cagaagttta cactattgcc cgtattcgct atgatgattt ggttgattta ccatattaca
    21181 gtcaggccta tcaagacagg ttggaccagc accaagaaga cgtagaaaag ttattggcta
    21241 acaatgatga acaacgattg gcagctattc agtcagatgg acagacagat atttcaaaag
    21301 gggaagagga aattgctcag gctcaatctc agcttgaaca ggcagagtca gaactggaaa
    21361 caggggagga gcaattagcg acaggtcgta gagagtttgc gcgtggtagg gctaaggttg
    21421 tccaaacgga agctgaatta cgagctgccc aagcccaact attaaaaaat aagtttgaat
    21481 tggaagaaag taaaaaggaa ttggacgggc gtaaggctca attagaacag actgcttcgt
    21541 ttctagacgg ctatcagatg gaattagaaa cgtcagccca gcagttagaa gaggccaagc
    21601 aagaattgga ttcccaaaat gcccaactga gtcagaccgc atctcagatt tccacagggc
    21661 gagctagttg gtttcaagct cagcaagagt tagaccttga aattgccaat cacttgcaag
    21721 aaggccaaac cctctcagac tatcctgact tgttggctag acaggaaggt ttggatgttg
    21781 agaaaagccg attagaccaa atggaatctg cacatgagca ggccaaccaa gcctatttac
    21841 aaggttatga ttactatcaa accaaacaaa atgaatacaa tagcaatcta gcccagtacc
    21901 aaacttggaa tcaagaatat caggcaggtt tggcccgtta tcaagcaggt ttgagtcagt
    21961 atgaacaagg cattgcagcc tacaataaag gagtagaaga ctatgaatgg ggactaagtc
    22021 agttagagtc ttcaaatcaa ctgctccgtc aggaagaatt ccgtttggag gaggctgata
    22081 aagagttaag tcaagctcaa tcccaatttt ctgagaagaa agccacggcc gatcaagaaa
    22141 ttagccaagc tcaaaccgag attgctcagg caaaatccga cttaagcaag ctggaaaaag
    22201 cgccctatca ggtctatact cgatcaagcc taccaggtgg agatggctat actacctata
    22261 gcaatgcgac taggtctatt gctgcagtgg gcaatgtctt cccagttgtt ctctaccttg
    22321 tcgcagctct agtgactttt accaccatgg cccgttttgt cgatgaagag cgaacacagt
    22381 caggtctctt caaggccctt ggctatacga atagacagat tatggctaag ttcattctct
    22441 atggtttgac ggcaggtctg gtaggaacaa tagtcggtat aatagcaggc aatctcctcc
    22501 tgtctccact tatttccaac attattacgc agacaaccgt gattggacca gctaaacttc
    22561 atttttatcc cctttggaca ggtttagcct tgctcctgtc tttggcttcc tcggtcctgc
    22621 cagcttactt ggtggctcgt cgggagctga cagaaaaacc tgctcagcta cttttaccta
    22681 agccacctgt tacaggctcc agcatttggc ttgaaaaatg gccggctatc tggtcccgcc
    22741 tgagcttcac ccataaggtg acagcccgca atatcttccg ctacaagctt cgtatgctta
    22801 tgaccatctt tggagtagca gggaccgtcg cccttctttt tggaggactg ggtatccgtt
    22861 cctccatctc aggagttgtc caacgacagt ttggggagct gatccattat gatatgttgg
    22921 tggtggaaaa cagcagagct actgaggaag agttggataa gttaacacat tttcttcagt
    22981 cagatcaggt tcgccaatcc ttgccagtgg cttttgaaca gctcaatcaa acggtggaag
    23041 aagacggtca gagaaagaat ttatcgatta gtctctacat ttcagaccgt cgggattttg
    23101 gaaatcaagt cagtctggaa agttcgacag gtcagcctat taaactgagt gacaggggga
    23161 ttgtcctcac agaaaaattg gcgcaaatct acggggtctc agttggtgat aagttatcac
    23221 tgaccttgga agataaggaa gtttcagtca gggtagaggc tgtggctgat atgtacgcag
    23281 ggcattttat ctatatgaca gatagctact acgaacaagt gaccggcaag cagaagactg
    23341 ccaatgccta tttggttcag ttgaaagaca gtcagttggg gcatattcag actcttgcaa
    23401 gtcaactcct agcgatgcca gcagtcagaa gtttggttca aaatacttct cttatagata
    23461 tgcttaccac gattgcaggc tcgcttcaga ccatcatgac cattctagtc atcctatcca
    23521 tcttgcttgg cttggttatt ctttacaacc ttacaattat caatatgtct gaacggattc
    23581 gtgaattatc caccatcaag gtactgggct tccacaataa agaggtgacc atgtacatct
    23641 atcgcgaaac cattgtcctt tcgctgattg gcatgttggt tggactggtt ggtggtattt
    23701 atctccacaa gctccttctt gccatgattg gttcggacag tatccgcttc aatccatctg
    23761 tcggactaga agtctatctc attcctatct tagccattag tggaattttg gctgttcttg
    23821 gttggtacgt caatcaccat ctgagaaagg tggatatgct ggaagccttg aagtcagttg
    23881 attaaaacag tctgggaata gactgtttta acccgagcct tgaaatcgga gagcgaggca
    23941 cagtcttggt ttggaaggaa ttttaaaaag agaatgacag tcttgcgatt caaaagaggc
    24001 tgggcaaaaa gccctggccc acttctcagt gttcgtgtca acatctcagc gcagtggttg
    24061 attggcagat ttgttcgtgt tttgcactcc aaatctggcc attacgactg atgcgaacag
    24121 agttcgcttc atttccaacc tccaacagtc actactctga ctgttggagc tatgcggggg
    24181 tgggagtgaa acagtctggg aatagactgt ttcagctcaa caactggaaa taaggacttg
    24241 ttgacgaact ctttttgact tagtcgagtt cttatacgaa aactcaaaag aatgcaaaaa
    24301 ctccctccct atgatataat caaagcgaaa aattttttat gttaggctac tagctcgtcc
    24361 ctagtagtct ttttctgtgc taaaaattca ggatacgtta tctgctcttt taagaggata
    24421 taagctattt ttaataattg atgtgcgagt gcaattgttg ctttttgtga accacgccga
    24481 ctttgaatct gataaaatcg gtctgctagg ggactacctt tttgtttttt gatagcgaaa
    24541 gcggcttggc ataaacattt cttcaaatat gaatttccgt gtcgaatctt ggtacttcgt
    24601 tttttaccag cactctcatt attacctgga caaagtcctg cccaagaagc tagatgtcca
    24661 gcagttggaa attgactcat gtcaacacca acctcagaaa tgataacaga agctgtaatg
    24721 acatcaatgc ctgggatgga atccaatatt tctacatggt tttcatattg tgatagatag
    24781 acattcattc gctcttccaa caactcaatc tgcttctgat aaaaatcata aatctccaag
    24841 gactgcttta acataaagcg gtgatggtca gaaaaatagc catccaaggc ttccagaagc
    24901 tgcggcactt tcttcttcaa gctggtataa acagattgat ggacaatgcg aggtgtaata
    24961 ggcgtcccat caaccagtag ctgaaggaga ttgcgaccag atagccccat aatatcttca
    25021 atataggttg ttagcttgat accgcctgac tgaagaattt tatggatacg gttggtttcg
    25081 cgattgcgac tttccacata atgttttcgc tgtctggtca attccctcaa ttcttgaata
    25141 gcttcatcgg ggacgaaact ccgaggcaat agaccaatcc gtgttaattt ggcaatccag
    25201 tgagcatcct tcttgtcggt tttctgacct ggaatagcct tcatgtgggc tggttgagcg
    25261 agtatcaact tgaagtcatc acatagagca tgccagacag gtcgccaata aacacctgtg
    25321 ctttccatac caacagcttc cacatgaaat tgactgagaa aatcgtggca agcacgtagg
    25381 cctttagtcg ttgtatcaaa tgtagccatc tcacgctttg gacgagttga ggtgagtggt
    25441 ccatgtagga tacagacgac aatattggct tgatggacat caatacctgc acaagattga
    25501 tagagaacat ccatgataat tctctccttc taaaaattag agagcttatc taccgaattt
    25561 tagtcgtatt tttatactca tgcctatcgc atattttggg gtgctcgtca gtagagtggg
    25621 ttagtttttc ccgcgatgac taacatcagt aactcgtcaa ccacaaagcc ctcactaaga
    25681 ttatacacct agtgagggct ttatgttcgt tttcattatt ctgtgggtga gggcggagcc
    25741 atcatggatg tctttcccac tcccaactct taaacaaaaa attctaccca agccccaact
    25801 agcaagaatg cgatgcagag atagatttgc caatttgccc tcaacatcca ttctttagac
    25861 caaaaggcac tgcgggctgt accgacagaa gcaaagagca agagcccaag ccagagggta
    25921 ggccccttgt ccaaccattc tccaatgcta ccaagcaaca acaatatcat caaataaaag
    25981 ttaaaactcc gataaaattc tttattaaat tctttaaaca tataaaatcc tcacagtaca
    26041 aagaaggaaa tgattgtcca actagcaatc aatccaatcg tcagataaat ttgccatgtt
    26101 tttttaatcc agctacgtga cttaaagaaa gctagataac cagcataaag tccgagtcca
    26161 agcttgagaa aatggataaa ccaaaacggt ccatttacag cagcagaaat cacttgtagg
    26221 atacaggcaa gagacaagta aaattcccag gtccgataga atgctttatt gaatttttta
    26281 gacatagtaa gctcctttct atttacccaa aataaatttc agatagataa tcaccacgat
    26341 caatccgata tagaagatgt agcccatgcg agtgagttct ttttcttcct tgaagaaaaa
    26401 ggttcgaatg gcatgaatta aaataatcat agaggccaat aggctatacc atgcaggcgt
    26461 ttgggaagga tctagcaata tgtctgacag aaaaaaacaa gtccaaaaaa tcatagaata
    26521 gaaggcgcta taacgataga aatgcttgtt aaactggaat ttcatgacaa atcacctctc
    26581 tttcttttat
//
LOCUS       DBNFEG010000010        26451 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.010, whole genome shotgun sequence.
ACCESSION   DBNFEG010000010 DBNFEG010000000
VERSION     DBNFEG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 26451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26451
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.010"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            2..985
                     /locus_tag="AC1PNG_000268"
     CDS             2..985
                     /locus_tag="AC1PNG_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HIP6700807.1"
                     /translation="MLEALVASNQEGLSGYGTDKYTESAIEKIRLATDCPHAQVTFLA
                     GGTQTNQIVISSMLASYEGVIAADTGHIAVHEAGAIEFTGHKVLALPHTDGKLVASEV
                     ENYISDFYADANHAHMVHPGMVYISHPTEYGIIYSKTELEDLSKVCQQHNIPLFLDGA
                     RLGYGLAARETDLDLKTIAELTDVFYIGGTKLGALMGEALVFTKNNQPKNFVSIVKHH
                     GALLAKGRVTGVQFDCLFTDDLYLKLGRHTIAMAEQLTQILEEKGFQFYLKSPTNQQF
                     VIVKNEELTRLTEAGIAYGFWEKYDDSHSIIRFATSWSTSQADIDELINIL"
     gene            complement(1004..1387)
                     /locus_tag="AC1PNG_000269"
     CDS             complement(1004..1387)
                     /locus_tag="AC1PNG_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700808.1"
                     /translation="MKYVILKTPVFVWLLIFALIQIFALSSFLPEPFDTRSLLPMGVL
                     TGCSIRVSSEVKDQDGQYAPFKYLWWVILGWTVIGILFGITIKAPLELHIALIVHAIA
                     GGIGYSLIERYAVYRYHYLQTKKKG"
     gene            complement(1448..1864)
                     /locus_tag="AC1PNG_000270"
     CDS             complement(1448..1864)
                     /locus_tag="AC1PNG_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6556 family protein"
                     /protein_id="HIP6700809.1"
                     /translation="MNTRKNTNKKPAGLTNFQKYLTFIGSILGIITACITIYTFTAKP
                     TTSSTSPSTTISSSSATSSPSQSSSDNPLTGVQDLTSSSSETTSSTQTETSSSVESTA
                     SSSSTETSATQASETRTETSTDTEVTSGASETTSNQ"
     gene            1977..3119
                     /locus_tag="AC1PNG_000271"
     CDS             1977..3119
                     /locus_tag="AC1PNG_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIP6700810.1"
                     /translation="MIYFDNAATTQVYPEVLKTYTEVATKIWGNPSSLHSLGSQATRI
                     LEASRKQIAELLGKDSKEIFFTSGGTEGDNWVIKGVAFEKAHLGKHIIVSAIEHPAVK
                     ESALWLKTQGFEVDLAPVNAQGFVDVSALESLIRPDTTLVSIMAVNNEIGAIQPIQEI
                     SQLLADKPTISFHVDAVQAIGKVPTERYLTDRVDFASFSGHKFHSVRGVGFVYVKAGK
                     KIAPLLTGGGQESDKRSTTENVAGIAATAKALRLTLDKAVDSQKRLAAMKQILVDELG
                     KYDDVTVFSGLEDFVPSIVTFGIKNIRGEVIVHAFEDHQIYISTTSACSSKAGKPAGT
                     LIAMGVPQKLAQTAVRISLDDDNDMGQIEQFLTIFKQIYQNTQKVR"
     gene            3121..4338
                     /gene="thiI"
                     /locus_tag="AC1PNG_000272"
     CDS             3121..4338
                     /gene="thiI"
                     /locus_tag="AC1PNG_000272"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HIP6700811.1"
                     /translation="MNYSEIMIRYGELSTKGKNKMRFINKLRNNIKHVLSVYPEVTVY
                     FDRDRGHVYLNGADYQEVSASLKKIFGIQNFAPSYKIEKSVPALKEAVVEIMQSIYKE
                     GMTFKIAARRSDHNFELDSRDLNQVLGDAVFTAIPNVQVQMKSPDITLRVEIRPDAAY
                     ISHEEIKGAGGLPVGTSGKGTLMLSGGIDSPVAGYLALKRGVEIEALHFASPPYTSPG
                     ALKKAHDLTRKLTAFGGNITFIEVPFTEIQEEIKEKAPEAYLMTLTRRFMMRITDRVR
                     EERGAMVIINGESLGQVASQTLESMQAINAVTNTPVIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNVEQYEARMDVEGLVERAVAGIIVTEITPKEE
                     VKDEVDSLIEDLL"
     gene            4457..5785
                     /gene="brnQ"
                     /locus_tag="AC1PNG_000273"
     CDS             4457..5785
                     /gene="brnQ"
                     /locus_tag="AC1PNG_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HIP6700812.1"
                     /translation="MMKKGALTGLLLFGMFFGAGNLIFPPALGVLSGENFWPAILGFV
                     VSGVGIAVIALIVGTLNPKGYVHEISRKISPTFATIYLVALYLAIGPFFAIPRTATTS
                     FEIGIAPLLGEANLGIWLFGFTALYFVAAYLIALNPSQILNSIGRILTPVFAILIVIL
                     VVLGIFKYGSTSPLPAAEAYSAGQAFGTGFIEGYNTLDALASIAFSVVAVNTLKHLGF
                     SSKKEYVSTIWSVGLVVALAFSALYVGLAFLGNHFPVPADVLASDTNKGVYILSQATQ
                     AIFGPGAQIFLAAMVIVTCFTTTAGLIVSSGEFFAERFPRFSYKVYATIFTLIGFGIA
                     NLGLNNIITFSVPVLLVLYPITICIVLIAIVNKFVPLSTYGMQLTVGVVTALSLVEVL
                     AGQFNWTAISKIISALPLAGQSLAWLLPALVGIVLSLFLPNKQESEVFEM"
     misc_feature    5853..5941
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5977..6291
                     /gene="rplU"
                     /locus_tag="AC1PNG_000274"
     CDS             5977..6291
                     /gene="rplU"
                     /locus_tag="AC1PNG_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIP6700813.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKLNVEAGQEVTFEEVVLVG
                     GEKTVVGTPLVAGATVVGTVEKQGKQKKVVTFKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            6338..6631
                     /gene="rpmA"
                     /locus_tag="AC1PNG_000275"
     CDS             6338..6631
                     /gene="rpmA"
                     /locus_tag="AC1PNG_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIP6700814.1"
                     /translation="MLNLNLANLQFMAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTKIYPGANVGRGGDDTLYAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            6823..6972
                     /locus_tag="AC1PNG_000276"
     CDS             6823..6972
                     /locus_tag="AC1PNG_000276"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6700815.1"
                     /translation="MFRGSGMGSNLVKLVIDDLANRNSKAFQIAVEEEDLGTWKLYKR
                     RIVYL"
     gene            7044..7958
                     /locus_tag="AC1PNG_000277"
     CDS             7044..7958
                     /locus_tag="AC1PNG_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIP6700816.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISIRDLEKELG
                     FQIFSRTSSGTFLTQKGMEFYEKAQALVKGFDQFEHLYLQPEEGEKTFSISSQHYDFL
                     PPLITEFSRSHPQYPHFRIFESTTVQILDEVAQGYSELGIIYLNERNTKGIMQKLDKL
                     HLQAVDLSAFQTHIYLREDHPLTKKAEVDPADLVGLPTVRFTQEKEAYLYYSENLIDT
                     SDSSVVFDVTDRATLNGILERTDAYATGSGYLDDESVNGITVIPLKGKVDNRMVYVKR
                     ANDELSSCAKDFIETMQAYFDKKKDENA"
     gene            7951..8415
                     /gene="lspA"
                     /locus_tag="AC1PNG_000278"
     CDS             7951..8415
                     /gene="lspA"
                     /locus_tag="AC1PNG_000278"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIP6700817.1"
                     /translation="MRKIGFPFLMVVLIGLDQFVKAWTVANIELDTVEPLLPGVISLA
                     YLRNYGAAWSILQNQQWFFTIMTIVAVTGLVWYYIKQIKGNIWTLFSLSLMIAGALGN
                     FIDRLRLGYVVDMFHLDFISFPVFNVADVCLTVGVGILFICIMKEESNGSKS"
     gene            8399..9292
                     /locus_tag="AC1PNG_000279"
     CDS             8399..9292
                     /locus_tag="AC1PNG_000279"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIP6700818.1"
                     /translation="MEVRVEVGGIRLDKALSDLTDLSRSVANEQIKAGQVLVNGQPKK
                     AKYSVQVGDVLTYQIPEVEEIDYVAEDIPLEIVFQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALLYHVKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHTKLAAELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKEAVTRFQVLERFGDYTLV
                     ELTLETGRTHQIRVHMAYIGHPVAGDEAYGPRKTLKGHGQFLHARTLGFTHPRTGEVV
                     EFTAEAPAIFQEALEKLRKAE"
     gene            9373..9711
                     /locus_tag="AC1PNG_000280"
     CDS             9373..9711
                     /locus_tag="AC1PNG_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HIP6700819.1"
                     /translation="METLPNCPKCNSEYVYEDGALLVCPECAYEWNPADVAEEESGPV
                     AIDANGNRLADGDTVTLIKDLKVKGAPKDLKQGTRVKGIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVKKI"
     gene            9759..10406
                     /locus_tag="AC1PNG_000281"
     CDS             9759..10406
                     /locus_tag="AC1PNG_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HIP6700820.1"
                     /translation="MKHQSILFYSRCLLAILAITGTALEIIKYGIGMLMYYTVQSNLL
                     VSLFAVYMVYAMSKGVDLQTSHFLRIKAAVTMSIMITCVVYHFMLAPLADDFWRLENL
                     LCHYIVPLYFLLDTLIVDRQQQYKWFDPIWWTLLPVLYMIFGLVNGLFIKIPIPDAKD
                     SPFAYFFLNVPKYGWTYVLTYAGVIFVAYLVCGFLLAGLKSINLKTSLSFGKNSQ"
     gene            10605..11126
                     /gene="pyrR"
                     /locus_tag="AC1PNG_000282"
     CDS             10605..11126
                     /gene="pyrR"
                     /locus_tag="AC1PNG_000282"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIP6700821.1"
                     /translation="MKTKEIVDDMTMKRAITRITYEIIERNKNLDNIVLAGIKTRGVF
                     IAKRIQERLKQLEGIDVPLGELDTKPFRDDMKVEDDTTNMTANVNDRDVILVDDVLYT
                     GRTIRAAIDNIVSLGRPARVSLAALVDRGHRELPIRADYVGKNIPTSRSEEIIVHMAE
                     IDGQDAVLLVEGA"
     gene            11182..12105
                     /locus_tag="AC1PNG_000283"
     CDS             11182..12105
                     /locus_tag="AC1PNG_000283"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIP6700822.1"
                     /translation="MTITNGKVSLKHLVTMETLSNEEVLGLIQRGIAFKRGEKVELDR
                     KYYASNLFFEDSTRTHKSFEMAELRLDMGMIDFDARTSSVNKGETLYDTILTMSALGV
                     DICVIRHSEVDYYKQLIDSPTIQTSIVNGGDGSGQHPSQSLLDLMTIYEEFGTFEGLK
                     IAIAGDITHSRVAKSNMQILKRLGAEIFFAGPEEWYAEEFDVYGQHLNIDDIVETVDV
                     LMLLRVQHERHDGDGGFSKETYNRLHGLTEERYKRLKDTAIVMHPAPVNRDVEIDDSL
                     VEAPKSRIVRQMQNGVFVRMAILEAIVNGKA"
     gene            12353..13432
                     /locus_tag="AC1PNG_000284"
     CDS             12353..13432
                     /locus_tag="AC1PNG_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HIP6700823.1"
                     /translation="MSKRRLILENGTIFEGEAFGADIDVTGELVFSTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDFESIKPTCKGVVVSEWARRASNWRNQMTLDEFLK
                     AKKIPAISGIDTRALTKIIRQHGTMKATLANVGDSVEHLTDQLRATVLPTNNIQQVST
                     KTAYPAPGVGRSVVLVDFGLKHSILRELAKRDCNVTVVPYDTTAEEILALNPDGVMLS
                     NGPGNPDDVPEALDMIRGILGKIPIFGICMGHQLFAKANGATTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVAREDLPECLMITHEEINDKSVEGVRHKYYPGFSVQFHPDA
                     APGPHDASYLFDEFMELMDSFKKGQ"
     gene            13942..17121
                     /gene="carB"
                     /locus_tag="AC1PNG_000285"
     CDS             13942..17121
                     /gene="carB"
                     /locus_tag="AC1PNG_000285"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIP6700824.1"
                     /translation="MPKRTDIKKIMVIGSGPIVIGQAAEFDYAGTQACLALKEEGYSV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKAGILDELGVELLGTKLSAIDQAEDRDLFKQLMEDLNQPIPESTIVTTVDEALEFAA
                     EIGYPVIVRPAFTLGGTGGGMCANEEELREIAENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDAADNALVVCNMENFDPVGIHTGDSIVFAPTQTLSDIENQMLRDASLSIIRALK
                     IEGGCNVQLALDSHSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     VNPVTGTTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVYHNEMPELSQVTDDQLVEKIVKAQDDRLFYLSEALRRGYTVEELAQ
                     LTKIDLFFLDKLLHILEIEQELATNFDNIDLLKKAKKYGFADRKIAELWGRTESYIRQ
                     LRTEHKIVPVYKMVDTCAAEFESATPYFYSTYEWENESIRSEKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTLEDVLNVIDL
                     EKPKGVIVQFGGQTAINLAEPLAKAGIPILGTQVADLDRAEDRDLFEKALKDLGIPQP
                     PGQTATNEEEALEAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPEH
                     PVLVDSYIVGRECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSKEVQA
                     TIADYTKRLAIGLDCIGMMNIQFVIKDETVYVIEVNPRASRTVPFLSKVTDIPMAQVA
                     TKLILGQSLAELGYQDGLYPESNQVHVKAPVFSFTKLAKVDSLLGPEMKSTGEIMGSD
                     LTLEKALYKAFEASYQHLAEFGNVVFTIADEDKEEVLGLAQRFYELGYGLLATAGTAA
                     FLTENGLAVTTVGKLGDDSRPDIPSLVRAGKVQAIINTVGKKRVADGDGQVIRSSAIE
                     GGIPLFTALDTAEAMVKVLESRSFMTQAI"
     gene            17185..17940
                     /locus_tag="AC1PNG_000286"
     CDS             17185..17940
                     /locus_tag="AC1PNG_000286"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIP6700825.1"
                     /translation="MIVTTSLGMDEGLVYRARRIASELGIEYKERKKQSVGKMLGTYE
                     AVLVLYKDKLILEQRGGQVLFFHPDTAMLRIKSGRDPLLELLGKEKQSIIDCTMSLGS
                     DSIVLASAGHRVTALESSKLVHFIVSRGLQDFDSGLQEVNRAMKSIQTIWTDSLTYLK
                     EQKDKSVDVIYFDPMFSEEIKESQNLSGLSTLADRSRLTEEIVSEAKRVARKKLIIKA
                     HFRDQVFEEFGFKRHVRPNQKFHYGEIILEEEV"
     gene            17937..18584
                     /gene="pcp"
                     /locus_tag="AC1PNG_000287"
     CDS             17937..18584
                     /gene="pcp"
                     /locus_tag="AC1PNG_000287"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HIP6700826.1"
                     /translation="MKIIVTGFDPFGGESINPALETIKSLPKTIAGAEIILVEIPTVF
                     DKAADVLEEKMAEHLPDAVLCIGQAGGRVDLTPERIAINQDDARIPDNEGQRPIDRTI
                     REDGQPAYFSTLPIKAMVEAIHRIGLPASVSNTAGTFVCNHLMYQALYLAEKQFPKTK
                     AGFLHIPFLPEQVVDKPGLASMSLNDIVRGVEVAIGAIVEYRDKEDIKKGGGSTH"
     gene            18584..18892
                     /locus_tag="AC1PNG_000288"
     CDS             18584..18892
                     /locus_tag="AC1PNG_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HIP6700827.1"
                     /translation="MKNIMEARKRVEEMEKIFNRQLELNQSLSDNMAQLNQEQSTYLQ
                     LLDYYQSQTYMEDLDLSNKGYFNGIPCGVLSEDGVYNLLFDRTNLASQLRELADMLEQ
                     "
     gene            19017..19289
                     /gene="rpsP"
                     /locus_tag="AC1PNG_000289"
     CDS             19017..19289
                     /gene="rpsP"
                     /locus_tag="AC1PNG_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIP6700828.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLL
                     AENSVTLKEERVLEWLAKGAQPSDTVRSLLSNAGVLKKFHEQKFSK"
     gene            19311..19553
                     /locus_tag="AC1PNG_000290"
     CDS             19311..19553
                     /locus_tag="AC1PNG_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIP6700829.1"
                     /translation="MDMIENLIIAIVKPLISQPDSLTIKIVDTPEFLEYHLDLDQSDI
                     GRIIGRKGRTISAIRTIVYSVPTSDKKVRLVIDEKE"
     gene            19629..21365
                     /locus_tag="AC1PNG_000291"
     CDS             19629..21365
                     /locus_tag="AC1PNG_000291"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6700830.1"
                     /translation="MNLIWTYLKKYPKWIALDLLGAFLFVVVNLGLPTFLARMIDQGI
                     TKNDVSQLYFWAGMMGLIVLLGIVGRVTLAYAAGKLTTNIVKDIRNDLYEKIQDYSHH
                     EYEQIGVSSLVARMTNDAFVLMQFSEMVLKLGIITPLMMIASVIMTLVTSPSLAWTVA
                     VAMPFLVFVIFYVATKTRPLSEKQQKRLDTINQYVRENLMGLRVIRAFTREEFQENRF
                     AEVNEEYTDISKKLFNLTGLTEPLFVQIIIAMIVAIVWFALDPLKTGSLQIGDLVAFI
                     EYSFHALFSFLLFANLFNMYPRMSVSSQRIQKVLDMPISISKNEEGITKSDTRGYLEF
                     ENVTFAYPGETESPVLHNISFKAKPGETIAFIGSTGSGKSSLVNLIPRFYDVTLGRIL
                     VDGVDVRRYNLKALRSKIGFIPQKALLFTGTIAENLKYGKMDASLAELHDATDVAQAK
                     EFIESKEEKFDTHLAEGGSNLSGGQKQRLSIARAIVKQPDIYIFDDSFSALDYKTDAI
                     LRSRLKEVTENATVLIVAQRVGTIMDADQIIVLNEGEIVGRGTHNELMESNEIYREIA
                     NSQLNRQSLTEE"
     gene            21374..23143
                     /locus_tag="AC1PNG_000292"
     CDS             21374..23143
                     /locus_tag="AC1PNG_000292"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6700831.1"
                     /translation="MKNRSVFTRVWDYLRRYQSSVFLAIFLKTISAVMNALEPFVLGL
                     IITELTKNLLDIANGVEGAQINYSYIAIMLAIYALRALLYEIGAYGSNYFMTKAVQGA
                     TKELRQDLSHKINKIPVSYFDKHQYGDLLGRFTSDVETVSNALQQSFLQLVNAVLTLS
                     LAIFMCFWLDVSLALVVITLVPVTYFGSKFVMGKSQPYFKQQADALGRLNGFVQENLT
                     GFNVLKLYGREEISTEEFRQITADLQEVGFKASFMSGLLMPLVHGFSNIAYVVVALLS
                     GLKVLAGTLTVGNMQAFVQYVWQISQPVQTLTQLAPQLQSAKSSLERIFSVLDELDEE
                     DANALELTESLTGQVSFEQVEFGYSEDKPLIRNFNLDVKPGEMVAIVGPTGAGKTTLI
                     NLLMRFYDVTSGAIKVDGQDIRNLSRQSYRSQFGMVLQDAWLYEGTIKENLRFGRLDA
                     TDEEIVEAAKAANVDHFIRTLPGGYNMEMNQESSNISQGQKQLLTIARALLADPAILI
                     LDEATSSVDTRLELLIQKAMKRLMKGRTSFVIAHRLSTIQEADKILVLKDGQIIEQGN
                     HESLLADKGFYYNLYQSQFSESK"
     gene            complement(23187..24110)
                     /locus_tag="AC1PNG_000293"
     CDS             complement(23187..24110)
                     /locus_tag="AC1PNG_000293"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIP6700832.1"
                     /translation="MKHSRRARRSRTQKKQLVPMLLGIALLVVTLLSIFLIFLKQPSK
                     QQSDSKASTVETTTTSSVMTSESSEVQTEIIWEQQTEPIKLPILMYHAIHVMAPEEEA
                     NANLIVDPTTFESHLKALQDAGYYTLSPEEAYKVLTENVLPAGKKVVWLTFDDSLWDF
                     YSYAYPLLKQYQMKATNNVITGFTEYGQAGYLTLDQIKEMQTAGLSFQGHTVNHPDLE
                     YSSIEDQTNELTSSKAYLDSQLNQETIAIAYPGGRYSADTVALTEQAGYKLGVTTNNG
                     LASASDGLLTLNRVRILPTTTAENLLAEIAY"
     gene            24215..25501
                     /locus_tag="AC1PNG_000294"
     CDS             24215..25501
                     /locus_tag="AC1PNG_000294"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019782768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIP6700833.1"
                     /translation="MVVKIGLLGFGTVASGVPFLLEENQEKIEKAAHDSIEVAKVLVK
                     DEAEKDRLLAAGHDYHFVTNIDDILADEDIAIVVELMGRIEPAKTFITHALEAGKHIV
                     TANKDLLAVHGSELRALAEEKGLALYYEAAVAGGIPILRTLVNSLAADKVTRILGVLN
                     GTSNFMLTKMVDEGWTYEKALQTAQELGYAESDPTNDVEGIDAAYKAVILSQFAFGMT
                     VDFEHVAHKGISNITPEDVAVAQELGYVIKLVGDIQETTSGIAAEVSPTFLPKNHPLA
                     SVNGVMNAVFVESIGIGQSMYYGPGAGQKPTATSVMADIIRIVRRLKDNTIGKAFNEY
                     SRSLQLAAPSDVKSEYYFSIDAPDKNGKMLRLAEIFNSEEISFKQILQQAADGETARL
                     VIVTHEMSKTQLENVTDKLSKETDFTVLNTFKVLGE"
     gene            25505..26365
                     /gene="thrB"
                     /locus_tag="AC1PNG_000295"
     CDS             25505..26365
                     /gene="thrB"
                     /locus_tag="AC1PNG_000295"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIP6700834.1"
                     /translation="MKIIIPATSANIGPGFDSVGVALSKYLTIEVFEETDEWVIEHNL
                     EHVPSDKNNLLIKTALKIEKGLQPHRIRMISDIPLARGLGSSSSVIVAGIELANQLAG
                     LNMTADEKLLKATEIEGHPDNVAPAIFGNLVISSYVNKKVQAVVTEFPEASFVAFIPN
                     YPLRTVESRGVLPSQMGYKKAVAASAIANVAVASLMAGDLEKAGKAIQSDMFHEPFRQ
                     LLVKEFCPIKQTAQELGAYATYLSGAGPTVMVLAPKDREDAIVLALEELNLDGTVHRL
                     QVDTKGIAIV"
BASE COUNT         7504 a         4959 c         6159 g         7829 t
ORIGIN      
        1 cttgctagaa gccttagttg caagcaacca agaaggttta tctggatatg gcactgataa
       61 atatactgaa tctgctatcg aaaaaatcag acttgctacg gactgtcctc atgcacaagt
      121 tacgttttta gcaggtggca cgcagaccaa tcaaattgtc atttcgtcta tgttagcttc
      181 ctatgaagga gtcattgcag cggatactgg tcatattgca gttcatgaag cgggggcaat
      241 tgaatttaca ggccataagg tgttggcctt accgcatacg gatggcaaac ttgtcgcaag
      301 tgaagttgag aactatattt ccgatttcta cgcagatgct aatcatgctc acatggttca
      361 tccaggaatg gtttatattt cccacccgac tgaatatggt ataatttata gcaaaacaga
      421 gctagaagat ttgtccaaag tttgccaaca gcataatatt ccgctctttt tagacggtgc
      481 tcgcttgggt tatgggcttg ctgctcgtga aacggatctt gatttgaaga caattgcaga
      541 gctgacagat gttttctaca ttggtggtac aaaactcggt gccttgatgg gggaagcttt
      601 ggtatttaca aagaacaacc agcctaagaa tttcgtatcg attgtcaaac accatggtgc
      661 cttgttggcg aaaggaagag tgacaggtgt ccagtttgat tgcctattta ctgatgactt
      721 gtatcttaaa ttggggcgtc atactatcgc aatggcagag caactgaccc agattttgga
      781 agagaagggc tttcaattct atctcaaatc cccgaccaat cagcaatttg ttattgttaa
      841 gaatgaagaa ttgactagat tgacagaagc tggaattgcc tatggcttct gggagaaata
      901 cgacgatagc cacagtatca tccgctttgc gaccagctgg tcaactagtc aagcggatat
      961 tgatgaattg ataaatatat tatagcaatc aaaaaagaaa gggttagcct ttctttttag
     1021 tttgtaaata atgatagcga taaactgcat aacgttcaat aaggctgtag cctattcctc
     1081 ctgcgattgc atgtacaatc agggcaatgt gcaattctag tggtgctttt atagtgatac
     1141 caaatagaat accgattaca gtccatccta gtatcaccca ccataagtac ttaaatggag
     1201 catactgacc atcctggtct ttaacttcac tggaaaccct gattgaacaa ccggtcaata
     1261 ctcccatggg gagcaatgaa cgagtatcaa atggttctgg taaaaatgac gataaggcga
     1321 agatttgtat aagcgcaaaa atcagtagcc aaacgaaaac cggcgttttt agaatgacgt
     1381 atttcatatt gcacacctcc tgaaattagt atatcaaatc caagaaagta aaacaaatat
     1441 ttttgtgcta ttgattagaa gtcgtttcgg atgccccact agttacctct gtatcagtac
     1501 tggtttctgt ccttgtctcc gaagcttggg tagcagacgt ttctgtagac gaggaagagg
     1561 cagtagactc tactgaagag ctggtctcgg tttgggttga ggatgttgtt tcggagcttg
     1621 aacttgttaa atcttgtact cctgttaacg gattgtcgct tgatgattgg ctgggactac
     1681 tagtggcgct ggatgatgaa atggtagtac ttggagatgt tgaggatgtt gtaggttttg
     1741 ctgtaaaggt gtagatagtg atgcaggcag tgataatccc taaaatagac ccgataaagg
     1801 ttaaatattt ttgaaagtta gttaaaccag ccggtttttt atttgtattc ttacgtgtat
     1861 tcatgatgta cctccacctt tagtttaaga taggaagttt aaaactatct taaagattgc
     1921 cgaaaggcat gaaaacatga taaaatagga ctattgcaat aaaaggaata taaaaaatga
     1981 tttattttga taatgcggca acgacacagg tctatccaga agttctcaag acctatacgg
     2041 aagtggcgac aaaaatctgg ggaaacccct ctagcctcca tagtctaggc agtcaggcaa
     2101 cccgtatttt ggaagcatct cgcaagcaaa tcgcagaatt actaggcaag gatagcaaag
     2161 aaatcttttt cacctcaggt ggtacggaag gtgacaactg ggtcatcaag ggagtagcat
     2221 ttgaaaaggc acatctgggc aagcacatca tcgtgtcagc tattgagcat ccagcggtaa
     2281 aagagtctgc actttggctc aaaacgcaag gttttgaggt ggatttggct ccggtcaatg
     2341 cccaaggttt tgttgatgtt tcagccttag aaagcttgat tcgtccagat accacactgg
     2401 tttctatcat ggccgtcaat aatgaaattg gtgctattca gcccattcag gaaatctccc
     2461 agctcttagc tgataagcca accatctcct tccatgtgga tgcagtgcag gcaatcggca
     2521 aggtgccaac ggaacggtat ttgacagacc gagttgattt tgccagtttt tcaggtcaca
     2581 aattccactc tgtcagagga gtaggtttcg tctatgtcaa agctggcaag aaaatcgctc
     2641 cgctattgac agggggagga caggaaagcg ataaacgctc aacaactgaa aatgtggctg
     2701 gtattgcagc aacggctaag gcgctccgct tgaccttgga caaggcggta gatagccaga
     2761 agaggctggc ggctatgaag cagattttgg tggatgaatt gggcaaatac gacgatgtga
     2821 cagttttttc tgggctagag gactttgtgc caagcatcgt aactttcggg attaagaata
     2881 ttcgtggcga ggtcattgtc catgcctttg aagaccacca gatttacatt tcgacaacct
     2941 cagcctgctc gtctaaggct ggaaaaccag ctggaacact gattgccatg ggtgttccgc
     3001 agaagttggc tcaaactgcc gtccgtatca gcttggatga tgacaacgat atggggcaaa
     3061 tcgaacaatt cctcacgatt ttcaaacaaa tttatcaaaa cacacagaaa gtaaggtaga
     3121 atgaactatt cagaaattat gattcgctat ggcgaattgt caactaaagg gaaaaacaag
     3181 atgcggttta ttaacaaact ccgcaataat atcaagcatg tcctttctgt ttacccagaa
     3241 gtgaccgttt attttgaccg tgaccgtggt catgtctatt tgaatggggc ggattatcag
     3301 gaagtttcag cttcattgaa gaagattttt gggatccaaa atttcgcacc gtcttataag
     3361 attgaaaagt ctgttccagc tttgaaagaa gcggttgtgg aaataatgca gtccatttac
     3421 aaggaaggga tgaccttcaa gattgctgcg cgtcgtagtg accacaattt tgaattggac
     3481 agccgtgacc ttaaccaagt ccttggagat gcagttttta cagctattcc aaatgtacag
     3541 gtacagatga agtcaccaga tattacctta cgagtggaaa tccgtcctga tgctgcttat
     3601 atttcccatg aagaaatcaa gggggcaggc ggtctgccag ttggtacatc tggcaagggt
     3661 acgctgatgc tatcaggtgg tattgattcg cctgttgcgg gctatttagc actgaaacgt
     3721 ggggttgaaa tcgaagccct gcactttgca agtccgcctt atactagccc gggtgcactt
     3781 aaaaaggccc atgatttgac tcgtaaattg actgcctttg gtggtaatat cacctttatc
     3841 gaagtgccgt ttacagaaat tcaggaagaa atcaaggaaa aggcacctga agcttatttg
     3901 atgaccttga cacgccgctt catgatgcgg attacagacc gagtacggga agagcgtggt
     3961 gctatggtta ttatcaatgg tgaaagtcta ggacaagtgg caagtcagac cttggaatcc
     4021 atgcaggcta tcaatgcggt gacaaataca cctgttatcc gtccagtcgt tactatggac
     4081 aagttggaaa tcattgatat tgctcaagaa attgatacct ttgatatttc cattcagcca
     4141 tttgaggatt gctgtaccat ctttgctcct gaccgtccaa aaaccaaccc taaaatcaaa
     4201 aatgtcgagc aatacgaggc tcgtatggat gttgaaggct tggtggaacg agctgtggct
     4261 gggattatcg taactgaaat cacaccgaaa gaagaagtga aagacgaagt ggatagttta
     4321 attgaagact tactgtagaa ttcaattgtt ttggcataca tttaaatcca gctattgctg
     4381 gattttttaa tacgaatcgt tcggaaaaat cgaatttgta tgatataata agacttcaga
     4441 aaaaaacaaa ggagttatta tgaaaaaagg agcactaaca ggattactcc tgtttggtat
     4501 gttttttggt gcaggaaact tgattttccc acctgcactt ggtgtcttat ctggtgagaa
     4561 tttctggcct gctattttag gatttgtcgt ttcgggtgtc ggtattgcag tcattgccct
     4621 gatcgtcggt accttaaacc caaaaggtta tgtacatgag atttctcgca agatttcacc
     4681 gacttttgca acgatttatc ttgttgcctt gtatttggct atcggtcctt tctttgccat
     4741 tccgcgtaca gcaacaacgt cctttgaaat tggtattgcg ccattgttgg gcgaagcaaa
     4801 ccttggaatt tggttgttcg gctttacagc tctttacttt gtggcagcct acctgattgc
     4861 cctcaatcca tctcaaatct taaatagtat cggacgtatt ttaacccctg tttttgcgat
     4921 tttgattgtg attttggttg tgctgggtat cttcaagtat ggttcgacta gtccactacc
     4981 agcagcagaa gcctattcag ctggacaggc atttggtaca ggatttattg aaggatacaa
     5041 cacccttgat gcccttgcct caattgcctt tagtgttgta gcggtgaata ccttaaaaca
     5101 ccttggtttc tccagcaaaa aggaatatgt atctaccatt tggtcagttg gtcttgttgt
     5161 agccttagct ttctcagcac tatatgtcgg tttggcattt cttggaaatc atttcccggt
     5221 acctgctgat gtcttggcgt ccgataccaa taagggtgtt tatattcttt cgcaagcaac
     5281 acaggctatt tttggaccag gtgcacaaat tttcttagct gccatggtta tcgttacttg
     5341 ctttacgaca acggctggct tgattgtttc ttcaggtgaa tttttcgctg agcgtttccc
     5401 acgttttagc tacaaagtat atgcgacaat ttttactttg attggctttg ggattgccaa
     5461 cctcggtttg aacaatatca tcaccttctc agttccagtt cttttggttc tctatccaat
     5521 tactatctgt attgtcttga ttgccattgt taacaaattt gtaccgcttt ccacttatgg
     5581 tatgcaatta actgtgggag tagtgacagc tttgtcattg gtagaagtgt tagcaggaca
     5641 attcaactgg actgctattt caaaaatcat ctcagctctg ccattggcag gacaatcatt
     5701 agcatggtta ttaccagctc ttgtcggaat cgtcctctct ctattcttac caaacaagca
     5761 ggagagcgaa gttttcgaaa tgtaagaaaa atgatacaaa acacttgcat ttgcaggtgt
     5821 ttttgttata ataggactgt tgactatgca cacatcctgt gcaaccgcac gaatgccgtt
     5881 ctttcgttga gagtagagta agtggttcgt cccacggtac taggcgagtc ttcacatcaa
     5941 attcgggcaa gacccaaaaa tcataggagg tgcataatga gcacatatgc aatcattaaa
     6001 actggcggca aacaagttaa agttgaagtc ggtcaagcta tctacgttga aaaattgaac
     6061 gttgaagcag gtcaagaagt tactttcgaa gaagtagttc ttgttggtgg tgagaaaact
     6121 gttgtgggta ctccactcgt agcaggcgct actgttgttg gtactgttga aaaacaaggt
     6181 aaacagaaaa aagttgttac cttcaagtac aaacctaaaa aaggtagcca ccgcaaacaa
     6241 ggtcaccgtc aaccttacac aaaagttgtt atcaacgcta tcaacgctta atcgcgtagc
     6301 ttgaagcaat cattacagaa attcggagga ataacatatg ttaaacttga atcttgctaa
     6361 cttgcaattt atggcccaca aaaaaggtgg aggttcaacg tcaaacggtc gtgattcaca
     6421 agcgaaacgc cttggtgcga aagctgctga cggccaaact gtatcaggtg gttcaatcct
     6481 ttaccgccaa cgtggtacaa aaatctaccc aggagctaac gtaggtcgtg gtggagatga
     6541 cactctttac gctaaagtag aaggcgttgt acgcttcgaa cgtaaaggtc gcgataagaa
     6601 acaagtatct gtttacccaa tcgcaaaata agtctgaaaa tggctttaca ataggctttc
     6661 cgagttgttt tcggagagtc ttttttgtaa tttgattttg ctatttttaa aagtgtacga
     6721 tagaagtgat ttaagacggc aatatcttct ctataggata aaaatatttt gccatctgca
     6781 ctgttgcgct gcctataagt tctacgttca gtagtagatg aaatgttcag aggaagtggt
     6841 atgggttcca acttagtaaa attagtcatt gatgatttgg cgaacagaaa ttccaaagcc
     6901 tttcaaattg cagttgaaga agaggatttg ggaacctgga agttatataa gagaagaata
     6961 gtctatttgt gaaaacgcaa gagttcagca aattcttgct ttttttgata taatggtaga
     7021 agcagtttta agaggtatca ggtatgaata ttcaacaatt acgctacgtt gtagccattg
     7081 ccaacagtgg tacttttcgg gaagcagcag agaaaatgta cgtttcccag cctagtttgt
     7141 ccatttccat tcgtgatttg gaaaaagagt taggttttca aatttttagc cgaactagtt
     7201 caggaacttt tttgacacaa aaagggatgg aattctatga gaaagctcag gctttagtta
     7261 agggatttga tcagtttgag catctctatt tgcaacctga ggaaggtgag aagacttttt
     7321 caatttcaag tcagcattat gattttcttc ccccgttgat tacggagttc tctcgtagcc
     7381 acccgcagta ccctcatttt cgcatttttg aatcgactac agtacagatt ttagatgagg
     7441 ttgctcaagg ttatagcgag cttggtatta tttatttgaa cgagagaaat acaaaaggaa
     7501 tcatgcagaa attggataaa cttcacttgc aagctgtgga tttgtctgca tttcagactc
     7561 atatctacct tcgtgaagat catcctttga caaagaaagc tgaggttgat ccggcagatt
     7621 tggtaggtct acctaccgta cggttcactc aagaaaaaga ggcttatctt tattattccg
     7681 agaacttgat tgatacgtcg gattcctcgg ttgtgtttga tgtgacagac cgtgcgactt
     7741 tgaatggaat tttggagcgt acggatgcct atgcaactgg ttcgggctat cttgatgacg
     7801 agagtgtcaa tggcattacc gtaattcctc tcaaaggaaa ggtagacaat cgcatggtct
     7861 acgttaaacg agctaatgat gaacttagtt cttgtgccaa agactttatt gaaaccatgc
     7921 aggcatattt tgataagaag aaggatgaaa atgcgtaaaa ttggatttcc atttttgatg
     7981 gtagtattaa ttggtttgga ccagtttgta aaagcttgga cagttgccaa tattgaatta
     8041 gacacggttg agccacttct tccgggggtt ataagtttgg cctatttacg aaattatggt
     8101 gcagcctggt ccatcttaca aaatcagcag tggtttttca cgataatgac aattgttgct
     8161 gtgacaggcc tagtgtggta ctatatcaaa cagatcaagg gcaatatctg gactttattc
     8221 agtctgtctt tgatgattgc tggtgctctt gggaatttta ttgatcgtct tagattggga
     8281 tatgtggtgg acatgtttca cttggatttc attagtttcc ctgtatttaa tgtagcagat
     8341 gtttgtctga cagttggtgt aggtatttta tttatttgta ttatgaaaga agagagtaat
     8401 ggaagtaaga gttgaggttg gtggtattcg tttggataag gcattgtcgg atttgacgga
     8461 tttgtctcgt tcggttgcaa atgagcagat taaggctggt caggttttgg tcaatggaca
     8521 gcctaaaaaa gctaaatata gtgtgcaggt tggggatgtc ctaacctatc aaattccaga
     8581 agtagaagaa atcgactatg ttgctgagga tattccttta gagattgtct ttcaggacga
     8641 ggatgttgct gttgtaaata aaccacaagg gatggtcgtt catccgtcgg ctggtcacac
     8701 gtctgggacg ctagttaatg ctctccttta tcacgtaaaa gatttgtcag gcatcaatgg
     8761 tgtcttgcga ccaggtattg tccatcggat tgacaaggac acatctggct tgctcatgat
     8821 tgctaaaaac gatgatgccc atacaaagct tgctgctgaa ttgaaagata agaaatccct
     8881 tcgtaagtat tgggccattg tacacggcaa tctaccaaac gatcgcggag taattgaggc
     8941 gccgattggt cgttctgaaa aagaccgcaa gaaacaagcg gtgactgcca aagggaaaga
     9001 agcggtgaca cgttttcagg ttttggagcg ttttggtgac tacactttgg tggaattgac
     9061 cttggaaaca ggacgaactc accaaattcg tgtccacatg gcttatattg gtcatccggt
     9121 tgcgggtgat gaagcttacg gcccaaggaa aacactaaaa gggcatggcc aatttcttca
     9181 tgccagaacg cttggattta cccatccgag aactggtgag gttgttgaat ttacggcaga
     9241 agcgccagct atttttcagg aggcacttga aaaactgcgc aaggctgaat aatagtatga
     9301 aaaccttatc atcttgccca ttttttcaac aaatgctata ctagaacatg aaaaatgtta
     9361 aggagagtaa gaatggaaac tttaccaaat tgtccaaaat gtaattctga atatgtctat
     9421 gaagacggtg ccctcttggt ttgccctgag tgtgcatacg aatggaaccc agcagatgta
     9481 gctgaggaag aaagtggacc tgttgcaatt gatgctaacg gtaatcgttt ggcggacggc
     9541 gatactgtga cgcttatcaa agacttgaaa gtgaaaggtg cgcctaagga ccttaaacaa
     9601 ggtacacgtg ttaaaggcat ccgtatcgtt gaaggcgatc acaatattga ttgtaaaatc
     9661 gatggttttg gtgccatgaa gttgaaatct gaatttgtta agaaaatcta agagagagga
     9721 ctggttaacc agtcttttcc tgtttttgaa aggatattat gaaacatcaa agtattttgt
     9781 tttacagtag atgcctgtta gccattttag ccatcacagg taccgcctta gaaatcatta
     9841 aatatggtat cgggatgctc atgtattata cggtacaatc taatttatta gtatctctgt
     9901 tcgctgtcta tatggtctat gccatgagta agggtgtgga tttacagacg tcacattttc
     9961 ttcggataaa ggctgctgta accatgtcta tcatgattac ctgtgtagtc tatcacttta
    10021 tgttggcacc tttggcagac gatttctggc gacttgaaaa tctgctctgc cattatattg
    10081 ttcccttata ttttttgtta gataccctaa tagtagatcg tcagcaacag tacaagtggt
    10141 ttgacccgat ttggtggacg cttctgcctg ttctctacat gatttttggt ctagtgaatg
    10201 gtcttttcat aaaaattcca atcccagatg ctaaggacag tccttttgct tatttctttc
    10261 tcaatgtccc taagtatggt tggacctatg ttttgactta tgctggagtc atctttgtcg
    10321 cctatctagt ctgcggtttt ctattggcgg gactaaaatc cataaacctt aagacttcat
    10381 tgtcttttgg aaaaaattct cagtaaaatc cgataatttc tatatttttt cagaaaaaaa
    10441 ttgtcaaggc tgagatttta tgctatactc tgcttaataa atcctttaaa acctgtccag
    10501 agaggcagac aaggagtcgg atgttaagca ggttggttct aactagccta tattttcata
    10561 gtctccttgt ataaggagac tttttctata cttttggagg tcttatgaag acaaaagaaa
    10621 tcgttgacga tatgactatg aaacgggcaa tcacccgcat cacttatgaa attatcgaac
    10681 ggaataagaa tttggataat attgtccttg cagggattaa aacacggggt gttttcattg
    10741 caaagaggat tcaggagaga cttaagcaat tagagggtat cgacgttccg ttgggagagt
    10801 tggataccaa gccgtttcga gatgacatga aggtagaaga tgatacgaca aatatgactg
    10861 ccaatgtcaa tgatcgtgat gtaatattag tagatgatgt gctttataca ggacgaacta
    10921 ttcgtgctgc tattgacaac atagtgtcct tgggccgtcc agctcgtgtt agtttagcgg
    10981 cacttgtgga tcgtggacac agggagttgc ctattcgagc agattatgtt ggtaagaata
    11041 tcccaactag ccgttctgaa gaaatcattg tgcacatggc tgaaatcgat ggtcaagacg
    11101 ctgtccttct ggttgaaggt gcatagtcgc ttcgtttaca aattctcatt ctacatctag
    11161 atcataaagg taggtatcat catgacaatt acaaatggta aagtttcact caaacacttg
    11221 gttacaatgg agaccctctc aaatgaagaa gtattgggac tcattcaacg tggtattgct
    11281 tttaaacgcg gtgaaaaggt agagttggat cggaagtact atgcttctaa ccttttcttt
    11341 gaggattcaa ctcggacgca taagtctttt gaaatggcag agcttcgttt ggatatggga
    11401 atgattgact ttgatgctcg taccagctca gtcaataagg gtgaaacctt gtatgatacc
    11461 attttgacca tgtcagcact tggggtagac atctgcgtga ttcgtcactc ggaagtggat
    11521 tattacaagc aattgattga tagtcctacc attcagactt caattgtcaa tggtggtgat
    11581 ggttcaggtc aacacccaag ccaatcccta cttgatttga tgactattta tgaagaattt
    11641 ggaacttttg aaggcttgaa gattgccatt gcaggtgata ttacacactc acgggttgct
    11701 aagtccaata tgcaaatttt gaaacgtctg ggagctgaga ttttctttgc tggaccggag
    11761 gaatggtacg cagaggaatt tgatgtctat ggtcagcatt tgaatattga tgatattgtg
    11821 gaaacggtag atgtactgat gttgctccgt gtgcaacacg agcgtcatga tggtgatggt
    11881 ggcttttcta aagaaactta taaccgtttg catggtttaa cagaggagcg ctacaaacgc
    11941 ttgaaagata ctgcaattgt tatgcatccg gctcctgtta accgtgatgt tgagattgat
    12001 gattctctag ttgaagcacc caaatcacgt attgtccgtc aaatgcagaa tggtgtattt
    12061 gtccggatgg cgattttgga agcaattgtc aatggcaagg cttaaaagta agctgttcag
    12121 ataccttaga actgagcagt tagaaatttg ttagacttat cttatttaat gtcgcccaga
    12181 gaggcgacga atagaaagga gaaggtacag gttcgagtaa gatttgattt ttatattcga
    12241 acacgcccta aggactgtgt gaaaaagata aatatcgact tgacgcctat agcgtcggcg
    12301 ccgaatttcc tattttcact ttgtcctttt aacgggcttt gtatcataga ttatgtcaaa
    12361 aagacgttta attttggaaa atggcactat ttttgagggg gaggccttcg gtgctgatat
    12421 tgatgtgact ggtgagttgg ttttctcaac tgggatgaca ggttatcagg aatccatcac
    12481 ggaccagtct tataatggtc agattttaac ctttacctat cccttggtag gaaattacgg
    12541 gattaaccgt gatgatttcg agtcgatcaa accgacttgt aagggagtgg ttgttagcga
    12601 gtgggctcgt cgagcaagta attggcgtaa ccagatgact ttggatgaat ttttaaaggc
    12661 taagaaaatt ccagcaattt ctggaattga cacacgcgcc ctaactaaaa ttattcgtca
    12721 gcacgggacc atgaaggcga cattggcaaa tgttggtgat tccgttgagc atttgacaga
    12781 ccagttgaga gcgactgttt tgccaaccaa caacatccag caagtatcga caaaaacagc
    12841 ctatcctgca cctggagttg gacgtagtgt tgttttggtt gattttggat tgaaacattc
    12901 tattcttaga gagttagcca agcgtgattg caatgtgaca gttgtccctt atgatacgac
    12961 tgctgaagaa attcttgccc tcaatccaga cggtgtcatg ctttcaaacg gtcctggtaa
    13021 cccagatgat gttccagaag ctttggacat gattcggggc attctcggaa aaattccaat
    13081 ttttggtatc tgtatggggc accaactctt tgctaaagca aatggggcaa cgacttataa
    13141 aatgaagttt ggtcaccgtg gtttcaacca tgctgttcgt gaaattgcga caggtcgagt
    13201 ggactttacc agtcagaacc acggttatgc agtggctcgt gaagatttac ctgaatgttt
    13261 aatgattact catgaagaaa tcaatgacaa atcagtagaa ggggtacgcc acaagtacta
    13321 tccaggtttc tctgttcaat tccacccaga tgctgcacct ggtccacatg atgccagcta
    13381 tttgtttgac gaatttatgg aattgatgga ttcgtttaaa aaagggcagt aaggtttaac
    13441 taatatcgtg ctaaacttta ctgcgtcggt cagagcaagt cggacttcat gttaacgcat
    13501 gtgtcttcct acgcaaagtc caatgggacc tttgctaact ctgacactag tttagaattt
    13561 caattattat cgaattggga ttctaaacgt cggacttatc ttatttaatg tcgctcagag
    13621 aggcgacgaa tagaaaggca agggacagat caatggttgc gagcaactca tttccctacg
    13681 cctgcctcta tggaggtcag gctatccctt gcactagctc gaaaatggat taatgtcgaa
    13741 tccattttct cacgtcggac aattatttaa ttctatccgg agagatagaa aatagaaagg
    13801 agagatacag gttcgagttt cctattcgaa cacgccctaa ggactgtgcg aaaaagataa
    13861 atgtcgtctt gatgcttgaa gcatcagtgt cgtgtttcct attttcgctt tgtcctctta
    13921 acgggctttg tatcatacat tatgccaaaa cgtacggata ttaagaaaat aatggtgatt
    13981 gggtctggtc cgattgtgat tggtcaggct gcggagtttg attatgctgg gactcaggct
    14041 tgtttagcct tgaaggaaga aggttacagt gtggtcttgg tcaattcaaa ccctgcgacc
    14101 attatgacag acaaggaaat agcagacaag gtttatattg agccaattac gcttgaattt
    14161 gtcacacgga ttttacggaa ggagcgtcca gatgctctct tgccaactct tggtggtcag
    14221 actggtctca acatggccat ggaattgtct aaggctggta ttttagatga gcttggagtt
    14281 gagttgttgg gaactaaact gtctgcaatt gatcaggcag aggaccgcga cctcttcaaa
    14341 caactcatgg aagatctcaa tcagcctatt cctgagtcaa cgattgtgac aacagttgat
    14401 gaggctttgg aatttgctgc tgaaattggc tatcctgtca ttgttcgacc agccttcacg
    14461 ctgggtggta ctggcggtgg tatgtgtgcc aatgaagaag aactacgtga aatagcagaa
    14521 aatggtctaa aattgtcacc agtgactcag tgtttgattg agcgttccat tgctggtttc
    14581 aaagaaattg aatacgaagt gatgcgggat gcggctgaca atgccttggt cgtatgtaac
    14641 atggaaaact ttgaccctgt gggaattcat acaggagatt ctatcgtatt tgccccaacg
    14701 cagacgcttt cggatattga aaaccagatg ttgcgtgatg ccagtctcag cattattcgt
    14761 gctctcaaaa tcgagggtgg gtgtaatgtg cagttggctt tggattctca tagcttcaaa
    14821 tactatgtca tcgaagtcaa tcctcgtgta tcgcgttcct ctgccttggc atccaaagcg
    14881 acaggttatc ctattgccaa attggcagca aaaattgcgg ttggcttgac cttggatgaa
    14941 atggtgaacc ctgttacagg aacaacctat gccatgtttg agcctgctct tgactacgtt
    15001 gtggcaaaaa ttccacgttt cccatttgat aagtttgaaa aaggggagcg tcgccttgga
    15061 acgcagatga aggcaactgg agaggtcatg gccattgggc gtaatatcga agaaagtctg
    15121 ctcaaggcct gccgttcttt ggaaatcggg gtataccaca atgaaatgcc tgaactcagc
    15181 caagtgacag atgaccaatt ggttgaaaag attgtcaagg cacaggatga tcgtttattc
    15241 tatctttcag aagcccttcg acgtggttat acagtagaag aactagctca attgacgaag
    15301 attgatcttt tcttccttga taagctgctt catatcttag aaatcgagca ggaattggca
    15361 acgaattttg ataatattga tttattgaaa aaagccaaga aatacggttt tgcagaccgc
    15421 aagattgcgg aactctgggg acggacagaa tcctacattc gtcaactgcg gacagaacac
    15481 aagattgtcc ctgtttacaa gatggtagat acttgtgcgg ctgagtttga aagtgctaca
    15541 ccttatttct attcaactta tgagtgggaa aatgagtcca tccgttcgga gaaagaatct
    15601 gttttggtgc tgggttctgg tccaattcgt atcggacagg gtgtggagtt tgactatgcg
    15661 acggtacatt ctgtcaaggc cattcaggca gctggctacg aagccatcat tatgaactca
    15721 aaccctgaaa ctgtgtcaac tgacttttcg gtttcggaca agctttactt cgagccgtta
    15781 accttggaag atgttttgaa tgtgatcgac cttgagaaac caaaaggggt tatcgttcag
    15841 ttcggtggcc agacggctat caatttggca gagccattgg ccaaagcagg tattccaatc
    15901 ttgggtacgc aagtagcgga tttggataga gctgaagaca gggatttatt tgaaaaagcc
    15961 ctgaaagacc taggaattcc acagccacca ggtcaaaccg caaccaacga agaggaagct
    16021 cttgaagcgg cccgcaaaat tggtttcccg gtacttgttc gtccctctta tgtcttgggc
    16081 ggtcgtgcta tggagattgt cgaaaatgaa gaggacttac gttcttatat gagaactgcg
    16141 gtcaaggctt cgccagagca cccagttttg gtggattctt acatcgtcgg tcgtgagtgt
    16201 gaagtggatg ctatttcaga tggtaaggat gtcttgattc caggtattat ggagcatatc
    16261 gagcgcgctg gggttcactc aggtgactcg atggccgttt acccgccaca aaccttgtcc
    16321 aaagaagtgc aggcaaccat tgcagactac accaaacgct tggcaatcgg tctcgactgt
    16381 atcggtatga tgaatatcca gtttgtcatc aaggatgaaa cggtctatgt gattgaggtc
    16441 aatccgcgtg ccagtcgtac ggtgccattc ttgtccaaag tcacagacat tccaatggct
    16501 caggttgcaa ccaaattgat tttgggacaa agccttgcgg aacttggcta ccaagacggt
    16561 ctttatcctg agagcaacca agtgcacgtc aaggcaccgg tcttctcgtt cacgaaacta
    16621 gctaaagttg acagtttgtt aggaccagag atgaagtcaa ccggtgagat tatggggtca
    16681 gacttgacac tggaaaaagc gctttataag gctttcgagg caagttatca gcacctggct
    16741 gagtttggca acgttgtctt taccattgcg gatgaagaca aggaagaagt gcttggtctg
    16801 gctcaacgtt tctatgaact tggttatggc ctactggcta cggcaggaac agccgccttt
    16861 ctaacagaaa atggacttgc tgtaacaact gttggtaaat taggcgatga ttcccgtcca
    16921 gatattccta gtcttgttcg ggcaggtaag gttcaggcta ttatcaatac ggttgggaaa
    16981 aaacgggtgg cagatggtga cggtcaagtc attcgaagct ctgctattga aggaggaatt
    17041 ccgctcttta cggccctcga tacagcagaa gctatggtca aagtgctgga gagccgtagc
    17101 ttcatgacac aggctatata gaagatacta tcaggatgag ctttgagttc gtcctgattt
    17161 tttgatatac tgaagagtat gaaaatgatt gtaacaacta gtcttgggat ggatgagggg
    17221 ctggtctata gggctagacg gattgccagt gaactgggaa ttgaatacaa ggaaagaaag
    17281 aagcagtcgg ttggaaaaat gttaggtacc tacgaggctg tcttggttct ttataaggat
    17341 aaattgatac tagagcagag gggtgggcag gttttatttt tccacccaga tactgccatg
    17401 ttgcggatta agtctggaag agatccgctt ttagaattac ttggaaaaga aaaacaatca
    17461 ataatagact gtactatgag cttgggatct gatagcattg tgttagcaag tgccggtcat
    17521 cgggtaacag ccttggaaag ttctaagcta gtgcacttta ttgtgagtcg aggtttacag
    17581 gattttgata gtgggctgca agaggtcaat cgagccatga aatctatcca gacaatttgg
    17641 acagatagtt tgacttattt gaaagagcaa aaagataagt ctgttgatgt catttacttt
    17701 gatcccatgt tttcggaaga aatcaaggaa tcacaaaatc tttctggctt gtctactttg
    17761 gctgacagaa gtcgtttaac ggaggaaatt gtatctgagg ctaaacgagt agctaggaag
    17821 aaattgatta taaaggctca ttttcgagat caggtctttg aagaatttgg ctttaaacgt
    17881 catgtcagac ccaatcaaaa atttcactac ggagaaatta ttttggagga ggaagtatga
    17941 aaatcatcgt aacaggcttt gatccctttg gtggcgagtc tattaaccca gccttggaaa
    18001 caatcaaatc tctacctaag accattgctg gtgcagagat tattcttgta gagattccga
    18061 ctgtttttga caaggctgcg gatgttttag aggagaaaat ggcagaacat ttacctgatg
    18121 cggtactatg tattggtcag gctggtgggc gagttgattt aactcctgag cgtatagcca
    18181 ttaaccaaga tgatgcacgc attccagaca atgaagggca gcggccgatt gatagaacga
    18241 ttcgagaaga tggtcagcct gcttatttct ccaccttacc catcaaagca atggttgaag
    18301 ctattcatag gattgggctt ccagcttctg tttctaatac tgctgggacc tttgtctgca
    18361 atcatctcat gtatcaagct ctttacttgg cggagaaaca atttccaaag actaaggcag
    18421 gctttctcca tattcccttc ttgccagagc aggttgtcga caagcctgga ctagcttcca
    18481 tgtctttgaa cgatattgta agaggagttg aagtagctat aggagcgatt gttgaatata
    18541 gggataaaga ggatattaaa aagggtggtg gcagtacaca ctgatgaaaa atattatgga
    18601 agcaagaaaa cgtgtagagg aaatggaaaa aatatttaac agacagctag agctgaacca
    18661 gtccctttcc gataatatgg cacaacttaa ccaggaacaa tcgacttatt tacaattatt
    18721 agattattac cagagccaga cctatatgga agatcttgat ttatctaata agggttattt
    18781 caatggtatt ccttgcggag tattgagtga agatggagtc tataatttac tgtttgaccg
    18841 tacaaatcta gcaagtcaac tgagagaact agcagatatg ctcgaacaat aaattttttt
    18901 aaaacctgtc aagttttttt cttgacaccc tgtcaaagtg tggtataata gaacatgtgc
    18961 taaatagctc tgctatttca ccgaataaaa atacaaagaa aagagaacat ttaaaaatgg
    19021 cagtaaaaat ccgtttgact cgtatgggtt ctaaaaagaa acctttctac cgtatcaacg
    19081 ttgcagactc acgtgcacca cgtgatggtc gtttcatcga aactgttggt acttacaacc
    19141 cacttttggc tgaaaactca gtaactctta aagaagagcg tgtacttgag tggttggcaa
    19201 aaggtgcaca accatctgat acagttcgta gccttctttc aaacgctggc gtattgaaga
    19261 aattccacga acaaaaattc tctaagtaag aacagtaagc gagaacactt atggacatga
    19321 ttgaaaatct cattattgcg attgtgaaac ctttgatttc acagcctgat agcttgacaa
    19381 taaaaattgt tgatacacct gaattcttgg aatatcattt ggatttggat caatctgata
    19441 ttggacgtat cattggtaga aaaggacgca caatttctgc aatcagaacg attgtctatt
    19501 ctgttccaac aagtgataaa aaagttcgtt tagtgatcga cgagaaagaa tagaaagtca
    19561 ccgtttggtg gcttttaacg ttgacaaagg ggcctagccc ctttttaaca tatagaaagg
    19621 aagaaatgat gaatctgatt tggacctatt tgaaaaaata tcctaagtgg attgccttag
    19681 acttgttggg ggccttctta tttgtagtgg taaacttagg cttgccgact tttttggcta
    19741 ggatgattga ccaaggaatt acaaagaacg atgttagtca gctctatttc tgggcgggga
    19801 tgatgggatt gattgtcctg ctcgggattg ttggtcgagt gacacttgcc tatgcagctg
    19861 gaaaattgac taccaatatc gtcaaagata ttcgaaatga tctgtatgag aaaattcagg
    19921 actattctca tcatgaatat gagcagattg gtgtttcttc tctagttgct cgtatgacaa
    19981 atgatgcttt tgttctcatg caattctctg aaatggtctt gaaactaggt attattacac
    20041 ccttgatgat gattgcttct gtcattatga ccttggtaac cagtccgagc ttggcttgga
    20101 cagtagcagt agccatgccg tttctagttt ttgttatttt ctatgtggca acaaaaactc
    20161 gtcctctctc tgaaaaacaa cagaaacgat tggatactat caaccagtat gttcgtgaaa
    20221 atctcatggg tttgcgggtc attcgtgcct ttacacgtga agagttccaa gaaaatcgtt
    20281 ttgcagaggt caatgaggaa tataccgata tttccaaaaa gctctttaat ctgactggat
    20341 tgacagagcc tctctttgtc cagattatca ttgctatgat tgtggccatt gtctggtttg
    20401 ctttggatcc cttgaaaaca ggcagtctac aaatcggtga tttagtagcc tttatcgaat
    20461 atagtttcca cgcgcttttc tctttcttgc tctttgctaa cttgtttaac atgtatccac
    20521 gtatgtcggt atcgagccaa cgaattcaga aagtcttgga catgcccatt tccatttcta
    20581 agaatgaaga aggtattact aaatcagata ctcgtggtta tttggaattt gaaaatgtaa
    20641 cttttgccta tcctggtgaa acggaatcgc ctgttctcca caatatttca tttaaggcta
    20701 agcctggtga aacgattgcc tttattggtt caacaggttc tggtaagtct tccttggtca
    20761 acttgattcc acgtttctat gatgtgacac ttggtcgtat cttggtggat ggtgtggatg
    20821 tcagacgcta caatctgaag gcactccgca gcaagattgg ctttattcca cagaaagctc
    20881 tgctatttac cggaactatc gcggaaaact taaagtacgg taagatggat gctagtcttg
    20941 ctgaacttca tgatgcgaca gatgtggctc aggctaagga gtttatcgag agtaaggaag
    21001 aaaaattcga cacccacttg gctgagggcg gaagcaacct gtcaggcggg caaaagcaac
    21061 gtttgtccat tgcacgtgct attgtcaagc agccggatat ttacattttt gatgattctt
    21121 tctcagcctt ggattataag acggatgcaa ttttgcggag tcgtttgaag gaagtgacgg
    21181 aaaatgcgac ggtgctaatt gtggctcaac gtgtcggaac cattatggat gctgaccaga
    21241 ttatcgtgct gaacgaaggt gaaatcgttg gccgcggcac ccacaatgaa ttgatggaaa
    21301 gcaatgagat ttaccgagaa attgccaact ctcaacttaa ccgtcaatcg ttaacagaag
    21361 aataggagga attatgaaga atagatcagt atttacccgt gtttgggact atttgcgtcg
    21421 ttaccaatct tctgtatttt tagccatttt tctaaagacc atcagtgcag ttatgaatgc
    21481 cttagaaccc tttgtgctgg ggcttatcat tacagaattg accaagaatt tgttggatat
    21541 tgctaatgga gtagaaggag cgcagattaa ctattcctac attgccatca tgcttgccat
    21601 ctatgccctt cgcgcccttc tatatgaaat cggtgcctac ggttctaact attttatgac
    21661 caaggcagtg cagggagcaa ctaaggaact acgtcaggat ttgagccaca aaattaacaa
    21721 gattccagta tcttattttg acaagcacca gtatggtgat ttactcggac gttttacaag
    21781 cgatgtggag actgtttcaa acgccctgca acaaagtttt ctccagctag tcaatgccgt
    21841 tctgaccctc tctctagcca ttttcatgtg tttctggttg gatgtatccc tagcacttgt
    21901 agtcataacc ttggttccag tgacctattt tggttctaag tttgtcatgg gcaaatccca
    21961 gccatatttc aagcagcagg ctgatgccct aggtcgtttg aacggctttg tccaagaaaa
    22021 tctgactggt tttaacgttc tcaaactcta cggccgtgaa gaaatttcta cagaagagtt
    22081 tcgccagatt accgcagatt tgcaggaagt tggtttcaag gctagtttca tgtctggttt
    22141 gctcatgcct ctagttcacg gcttctcaaa catcgcctac gttgtcgtcg ccctgctatc
    22201 tggattgaaa gttcttgcag gtacattgac ggtgggtaat atgcaggcct tcgtccaata
    22261 cgtctggcag atttctcagc cagtccaaac cttgacccaa ttggcaccac agttgcagtc
    22321 tgccaaatct tcccttgaac gcatcttttc agtcctagat gagcttgatg aagaagacgc
    22381 caatgcgctg gagttgacag aaagtctgac aggacaagtc agctttgaac aggtcgaatt
    22441 tggctattca gaagacaagc cgcttatccg taactttaat cttgatgtta agccaggtga
    22501 aatggttgct atcgttggac caacgggggc aggtaagaca acccttatca acttgctcat
    22561 gcgtttctat gatgtcacat caggtgctat caaggtagat gggcaggata ttcgaaatct
    22621 ttcacgtcaa tcctaccgta gtcaatttgg catggtcttg caggatgctt ggctctatga
    22681 agggaccatc aaggaaaacc tgcgttttgg acgtctggat gcgacggatg aggaaattgt
    22741 ggaggctgct aaagctgcca acgttgacca ttttatccgt accctgcctg gtggttataa
    22801 tatggaaatg aaccaggaat ccagcaatat ttcccaaggt cagaaacagc ttttgaccat
    22861 tgcgcgtgcc ttgttggcgg atcctgctat tctgattctt gacgaagcga cttcttcagt
    22921 tgatacccgt ttggaactct tgattcaaaa ggctatgaaa cggttgatga aagggcggac
    22981 aagtttcgtc atcgctcatc gcttatctac cattcaggaa gctgataaga ttttggtctt
    23041 gaaagatgga cagattatcg aacaaggtaa ccatgaaagt cttttggcag acaaaggttt
    23101 ctattataat ctttatcaaa gccagtttag tgaatccaaa taaaaaaacg aactcccttg
    23161 tcagtgaaga caggggagtt tagtgtttaa taggcgattt cagctagtag attttctgcg
    23221 gtggtggtcg gtagaattcg aacacgattg agagtcagta atccgtcact agcagaagcc
    23281 aagccgttgt tagtagtcac accaagtttg tagccagctt gttcagttaa ggctactgta
    23341 tctgctgagt atcgtccacc agggtaggcg atagcgatgg tttcctgatt taattggctg
    23401 tcaagatagg ctttagagga agtaagttca tttgtttgat cctcgataga actatactct
    23461 aaatcagggt ggttgacggt atgcccttga aaggagagcc ctgcggtctg catttccttg
    23521 atttgatcaa gagtaaggta ccctgcttgt ccatattcag taaatcctgt aatgacattg
    23581 ttggttgctt tcatttgata ttgcttaagc aatgggtagg cataactata aaaatcccag
    23641 aggctatcgt caaaggttag ccagacaacc tttttgccag caggaaggac attttctgtc
    23701 aataccttat aagcctcttc aggtgataaa gtatagtaac ctgcatcttg caatgctttt
    23761 agatgacttt cgaaagtcgt tggatctacg ataagattcg cattagcttc ttcctcagga
    23821 gccattacgt gaatggcatg atacatgagg attggaagtt tgataggttc agtttgttgt
    23881 tcccaaatga tttctgtttg tacttcacta ctttctgaag tcataacact tgaagtggtt
    23941 gttgtctcta ccgttgaagc tttggaatca ctttgttgct tagatggctg ttttagaaag
    24001 attagaaaaa tagatagtag agtcactact aataatgcga tgccaagtag cattggcaca
    24061 agttgtttct tttgtgtgcg gcttcgacgg gctcttcttg aatgtttcat tatcttatct
    24121 ccataatcat atattaaaac aattatatca aattttttgc caaatagtca gaatatttgt
    24181 tataatggga gaaatacatt ttaaaggaga gcacatggta gttaagatag gattattagg
    24241 atttggaacc gttgcgagtg gtgtgccatt tttattagaa gaaaatcagg aaaaaataga
    24301 gaaagctgcg catgatagta ttgaagttgc taaggttttg gtcaaggatg aagcagaaaa
    24361 agaccgtctc ttggctgcag gtcatgacta tcattttgta acaaatattg atgatatttt
    24421 agctgatgag gacattgcca tcgtcgttga gctgatggga cgtattgagc cagcaaaaac
    24481 atttattaca catgccttgg aagcaggcaa gcatattgta acagccaata aggatttact
    24541 tgctgttcat ggtagcgaat tgcgtgcctt ggctgaagag aaaggtcttg cgctttatta
    24601 tgaagctgca gttgcaggtg gtattccaat cttgcgtacc ttggttaact cattggctgc
    24661 tgacaaggtg actcgtatcc tcggtgtatt gaatggtact tcaaacttca tgttgactaa
    24721 gatggttgat gaaggctgga cttacgaaaa agctcttcaa acagcacagg aacttggcta
    24781 tgcagaatct gacccaacaa atgacgttga gggaattgat gcggcttaca aggctgttat
    24841 cctcagtcaa tttgctttcg ggatgacagt tgattttgaa cacgtagcac acaagggtat
    24901 ttcaaatatc acgccagaag atgtagcagt ggcacaagag ttgggctatg tcatcaaact
    24961 tgtgggtgat attcaagaaa ctacctctgg tattgctgcc gaagtatctc caaccttcct
    25021 accgaaaaat catccacttg caagcgtgaa tggtgtgatg aatgctgtat ttgtcgaatc
    25081 tatcggcatc ggccagtcta tgtactatgg accgggtgct gggcaaaaac cgactgcaac
    25141 aagtgttatg gctgacatta ttcgtatcgt tcgtcgcttg aaagacaata ctatcggcaa
    25201 agcttttaat gaatatagcc gctcacttca attggcagcc ccaagcgatg tgaaaagcga
    25261 atattatttc tcaattgatg cccctgataa aaatggaaaa atgcttcgtt tagcagaaat
    25321 cttcaattct gaagaaattt ccttcaagca aattctgcaa caagctgcag atggagaaac
    25381 agcccgccta gtgattgtga cccatgaaat gagtaagact cagttggaaa atgtaacaga
    25441 caaattgagt aaagaaacag actttactgt cttaaatacc tttaaagtac tgggagaata
    25501 agcaatgaaa attattattc cagcaacttc agctaatatc ggtccaggat ttgattcagt
    25561 aggtgttgcc ctttcgaaat atctaacgat tgaagtattt gaagagactg atgaatgggt
    25621 gattgagcac aatcttgaac atgttccatc agacaagaac aatcttttga tcaagacagc
    25681 tttaaagatt gaaaaaggat tgcagccaca tcgtatcaga atgattagtg atattccctt
    25741 ggcacgtggt ttgggttcat ccagttccgt tatcgtagct ggtatcgaat tggctaatca
    25801 attagcaggt ttgaatatga cagcggatga aaaattactc aaagctacag aaattgaagg
    25861 acatcctgat aatgtagccc cagcgatttt tggtaattta gtcatctcta gctatgttaa
    25921 taagaaagta caggcagttg taacagaatt cccagaagca agttttgttg cctttattcc
    25981 aaactaccca ctgcgtacgg ttgaaagccg aggtgtactg ccttctcaga tggggtacaa
    26041 gaaagcagtg gctgctagtg ccattgcaaa cgttgctgtt gccagtttga tggcaggtga
    26101 tctggaaaaa gctgggaaag caattcagtc agacatgttc catgaaccgt ttcgtcagct
    26161 acttgttaag gagttttgtc caatcaagca aactgcacag gagttgggag cctatgcaac
    26221 ctacttatca ggtgccggtc caacagtgat ggtattagcg cccaaagata gggaggatgc
    26281 tattgttcta gcattagagg aactaaatct agacggaact gtccaccgcc ttcaagttga
    26341 taccaaaggg attgctattg tatagatagt gagaatctat catttgcaaa actaaacagg
    26401 tttattttat tggagttcgc catcgctact atatgcggta ggaagggaac t
//
LOCUS       DBNFEG010000011        22695 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.011, whole genome shotgun sequence.
ACCESSION   DBNFEG010000011 DBNFEG010000000
VERSION     DBNFEG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22695
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.011"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(158..970)
                     /gene="purR"
                     /locus_tag="AC1PNG_000296"
     CDS             complement(158..970)
                     /gene="purR"
                     /locus_tag="AC1PNG_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HIP6700835.1"
                     /translation="MKLRRSERMVVISNYLINHPYELTSLNTFAEKYESAKSSISEDI
                     AIIKKAFEESSIGHIETITGASGGVVFTPSISKAESVEIAQALRDQMAESNRILPGGY
                     IYSSDLLSTPHILKNVGRIIADAFKDEKIDAVMTVATKGVPLANAVANVLNVPFVIVR
                     RDLKITEGSTVSVNYVSGSSDRIEKMFLSKRSLKAGSRVLIVDDFLKNGGTINGMISL
                     LSEFDSTLVGVAVFAENQKGDRSVANYKSLLAVTDINVKENRVDVELGNIFE"
     gene            complement(1194..1931)
                     /locus_tag="AC1PNG_000297"
     CDS             complement(1194..1931)
                     /locus_tag="AC1PNG_000297"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIP6700836.1"
                     /translation="MAVRIVSRQPLTKGWSTDRKYKVRLEDGRLGLLRIAERPAYEAK
                     RLEFQLVKNLFGLGLPVAEPIALWVGEESVYTLYEWVEGQDMNEVATSLSDSVLYDLG
                     CQSGQFLRTLHALPIDQSQRDWNSFYQAKIDNKLAVYQAASHSYPNGQAMIDFVQANR
                     HLLEGRPYHHGDFHTGNFLLGADGKLKILDFDRYDIGDPWEEFNRLIFTADLSPAFAR
                     GQVDSYFDGAIPEEFWRLMALYVTVIV"
     gene            complement(1931..2872)
                     /locus_tag="AC1PNG_000298"
     CDS             complement(1931..2872)
                     /locus_tag="AC1PNG_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HIP6700837.1"
                     /translation="MKINQMKKDEFFEGFYLIKTAEVRQTRAGKDYLALTFQDDTGEI
                     EGKVWDAQPGKIKDFTAGTVVHMQGRREVYNNTPQVNQLVLRLPKAGEPNDPADFKEK
                     PPVDVKDTKDYLSQMIFRIENATWQRIVRALYSKYDKEFYSYPAAKTNHHAFYSGLSF
                     HTATMVRLADKIGDIYLQLNKSLLFAGIMLHDLAKVIELTGPDNTGYTVRGNLIGHIS
                     LIDEEITKVLMELGIDDSLEEVTVLRHVILSHHGLLEYGSPVRPQIMEAEILHMIDNI
                     DAEMMMMLSALDKVGPGEMTNRIFAMDNRAFYKPNID"
     gene            complement(2862..4100)
                     /locus_tag="AC1PNG_000299"
     CDS             complement(2862..4100)
                     /locus_tag="AC1PNG_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIP6700838.1"
                     /translation="MDIVLLILLILVLLALVFLYGKWQSLALLLQDQAEDTADNLSDQ
                     LSYQLENATLKQQQAIHQEVERLRTELYQQLTDIRQELNRSHLETRDATDRRLQAIQE
                     SNEKRLKEMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVARDVGSLNK
                     VLSGTKTRGIMGELQLGQIIEDILTPSQYEREFATVSGSSERVEYAVKLPGRTEGDYI
                     YLPIDSKFPLADYYRLEDAYESGDKDQIDLHRKNLLAAIKRFAKDIQSKYLNPPETTN
                     FGVLFLPTEGLYSEVVRNPIFFDELRRQENIVVAGPTTLSALLNSLSVGFKTLNIQRS
                     ADDISKVLGNVKLEFGKFSDLLLKAQKQLNQASSNIDKLLTTRTNAIERSLRTIDLYE
                     DDQTKGLLGLSPLDEEDNEN"
     gene            complement(4101..4733)
                     /locus_tag="AC1PNG_000300"
     CDS             complement(4101..4733)
                     /locus_tag="AC1PNG_000300"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIP6700839.1"
                     /translation="MIKIAVIAGGSFDCFPEPADLYVGVDAGSLRLLDHFLPLDWAIG
                     DFDSVTSEELGRIKDQAERFLQAPAEKDDTDLELALKEVFKVYPQAQVRIYGALGGRM
                     DHMMANLFLVAEPDLAPYMEQIELVDSQNIVRFRPAGQHRLSPIAGMKYISFMPSDQS
                     RLTIRHAKYPLDASNYFFKKCYASNEFIDRDIDIQLDQGYVVLIYSKDKN"
     gene            complement(4726..5385)
                     /gene="rpe"
                     /locus_tag="AC1PNG_000301"
     CDS             complement(4726..5385)
                     /gene="rpe"
                     /locus_tag="AC1PNG_000301"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIP6700840.1"
                     /translation="MSHYKIAPSILAADYANFEKELKRLEKTGVEYVHIDVMDGHFVP
                     NISFGADVVAAMRPHSKLVFDCHLMVSNPENHIETFARAGADILTIHAEATVHLHGTL
                     QKIRAAGMKVGVVINPGTPLVTIEPVLNLVDQVLLMTVNPGFGGQAYIPEVAEKIADL
                     VKLREAKGLNFDIEVDGGIDDKTIHSAKNAGANVFVAGSYLFKGDLDANVAKLRAALH
                     D"
     gene            complement(5400..6275)
                     /gene="rsgA"
                     /locus_tag="AC1PNG_000302"
     CDS             complement(5400..6275)
                     /gene="rsgA"
                     /locus_tag="AC1PNG_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIP6700841.1"
                     /translation="MQGRIIKALAGFYYVEADGQIYQTRARGNFRKKGQTPYVGDFVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSAKEPDFNANLLDRFLVLLEQK
                     DMDPIIYISKMDLVEDRTEMDAFKAIYEKISYPFVYSLEELTPLLQDKVTVFMGQTGV
                     GKSTLLNRIAPELALETGAISDSLGRGRHTTRAVSFYNVFGGKIADTPGFSSLDYEVK
                     EAEALTDCFPEIAESSQDCKFRTCTHTHEPDCAVKEAVASSAISQSRFDNYLQFLSEI
                     QNQRETYTKVSKKFK"
     gene            6400..6481
                     /locus_tag="AC1PNG_000303"
     tRNA            6400..6481
                     /locus_tag="AC1PNG_000303"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6433..6435,aa:Leu,seq:cag)
     gene            complement(6755..8332)
                     /locus_tag="AC1PNG_000304"
     CDS             complement(6755..8332)
                     /locus_tag="AC1PNG_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP6700842.1"
                     /translation="MKKRNFWLTLTCIFAVALLNVGFLYQYRYIIDAIAAGDRQAFVS
                     YFVLMGLTVLVMLVFEYIRQIANVSYLNQVGFQLQATFIGKIFDLPFRQFVEQGAGAY
                     ISQLNNDLETVKEDYYDAFFTIFQGACTFGIASLALLSLDGLTAIFLILISFLPVVIP
                     YLFKKRRRLNQEAISDSQQVYNTRVSDVFLSYLQVKNSHQRQQVLDGLNDRYQAVNDK
                     VNQATRTTVTMRLLVGFVFYLTTLSIIFIGGFQVFSGALTIGGLTAILTISEQLVDPI
                     NSIAAAFLDRHAVKKLKQEFELSTFGQETKGQELASAFQSIQLVKASYAVGEKQVFED
                     LTVAFERGKKYLILGESGSGKSSLALILTKNSQLQAGDIYVDKTSLSDLSYQAIQDKI
                     AYLPQEGSLFHDTVLYNLTMGREIPEDRLMFLIKTMNLDSRFPSYASLSEEISDDSGL
                     SGGQKQRLLLIRALLQDKDILLLDESLSALDQETYAVIENYLTSLADKTLIHISHRMS
                     EEVLSRYDGVVRIGESN"
     gene            complement(8622..11066)
                     /locus_tag="AC1PNG_000305"
     CDS             complement(8622..11066)
                     /locus_tag="AC1PNG_000305"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIP6700843.1"
                     /translation="MKQIKLKATKQFILVMVMTVLLAMGRALLLSDVSFPLWFIPLPL
                     IAYLIFTFYVLVLLDKYEKFIKTKTFAKYVSYFLGFLYLVNLVYRLNAKKYQPWNIFR
                     NNLFQFELLLMLALPVLLAFLWRKKTGIREKLSQWSANHLVPDGYLLLTSLLSLSPLA
                     ISYWKDSHYESLVEKGSYIEFFSQTPLFAPIHFIGTYIFLRYLHKAFVEFKANRTNVH
                     SMLFISLALATLSHIGYQASMAGATGSYFTRHLFPGAIVFQIACLFFLNVIISLVINR
                     QILSVAVIASLNVILVTANFLKFRYRSEPLTPNDFKWVGNLGMILSFISLRVILVSLV
                     FIVLFVFIYRHIHKKYFRGQIVAAIWKRLAGISVIVSLILGMGWAIRNEKDHKIAGWI
                     PILSQVNNWRNVDWKGYAFVARYRTLSFLWLQQLSKTSMEMPENYSEKTMKAIVKKYT
                     ALAEEINAERTGQLTDQTVIYILSESLADPRRIPGVTLSQNVLPNIEYIMSQTTSGLM
                     KSDHYGGGTANIEFQVYSGLPFYNYSSSISSVYLDVAPNMKKLPSISDLYPAENRVAI
                     HPYFDTSYNRNSIYKQLGIEQFYTLNSAKYPLAVTAEDYQGNFVSDKKTYDLILEQVR
                     SGTNTFVSAITMQNHVQWNSAEPASITASGEGFTAEENANLNSYVRLLSFTDQATRDF
                     LDQLKTLDKKVTVVFYGDHLPGLYPESAFVTDPSAQYKTDYFVWSNFETEDYHYDLVN
                     SSDMNALMLETTNNKVSPYYALLTEVLHKDRVGQAERDAKVAEELKLVQYDLSTGKGY
                     LLKYKDFFKVATETTE"
     gene            complement(11079..12809)
                     /locus_tag="AC1PNG_000306"
     CDS             complement(11079..12809)
                     /locus_tag="AC1PNG_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700844.1"
                     /translation="MLKKLLKKHPDIVAVTVFFLFSLTLLLPQILSKAVVTGDDIVFH
                     YNRFYDTAMQIKSGDFSYIMSLHGYQQSGRIVNALYGPYLAYLQGALLLLAGTWYRYQ
                     LLSNLLLGVLSATSLYFLMREVKVRYSLSVGLSMFFVTTYSVQYWWVAQGFSSWGVAL
                     FPLCLIPAVRFLKTGKVPIFLMAGAVGLMLQIHMLSALFLILAYAILFSIGWLKSQDK
                     WNVMKDVLFSVGVFLILTVNIWLPLLYVNATNELAAPFINKKFIQSTVTWSKAYFLYF
                     PYSLPIIFATFLYFMLKKWKKQSVILRGLTVTFLVFFIFSTNLFPWQLFAGKGIGLVD
                     LIQFPFRFFLYANALLLTIVGLQLTDYEVRKKLFLGLTILSTIIGVGFTWDQYNQAVN
                     DQYNKAEFLQDMLHTTLYGTTEELRASLHSKNLGEFVQLAQKSTPDYVPVLSEKEENV
                     RAVNEAKSRYDRYRDQIILPNQQFNKQVFGDSLKISWSANQSEEIQIPIVIYRDSELT
                     LNGKRLGKMDYYLSTIGVPTVTSQKGKNTLILSYKQQWWLLPVIFISLTGWILWSIYY
                     TFIYRKSNIS"
     gene            complement(13172..14044)
                     /gene="rsmA"
                     /locus_tag="AC1PNG_000307"
     CDS             complement(13172..14044)
                     /gene="rsmA"
                     /locus_tag="AC1PNG_000307"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIP6700845.1"
                     /translation="MRIADKNVTRAVLERHGFTFKKSFGQNFLTDTNILQKIVNTAEI
                     DQNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLIPILADTLGRFDNVTVVNQDIL
                     KTDLQTQIQNFKNPDLPIKVVANLPYYITTPILMHLIESKIPFSEFVVMMQREVADRI
                     SAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRREQPLVSVKD
                     EDFFFRVSKASFVHRRKTLWNNLTNHFGKSDEVKDKLTRALGMAELEASVRGEALSMA
                     DFARLSDSLQEVGL"
     gene            complement(14064..14594)
                     /locus_tag="AC1PNG_000308"
     CDS             complement(14064..14594)
                     /locus_tag="AC1PNG_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HIP6700846.1"
                     /translation="MKKVVLLRGVTPVGKNKIPKMSYLAEILTEAGLTSVQTYIQSGN
                     VVCETDILDEELSRLIHQTIKEKIGAELAIIVKHQMELTQAVAENSFGEGYDPSRVHL
                     VFTNDEINGEKLNKLLHQDFGDEELRLGSQCLYMYLPRDARKKKLNTNYLEKQLDITA
                     TMRKLSVISRLSEMAK"
     gene            complement(14608..14865)
                     /locus_tag="AC1PNG_000309"
     CDS             complement(14608..14865)
                     /locus_tag="AC1PNG_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HIP6700847.1"
                     /translation="MGIHFTDDAWEDYLYWQGQDKKTLKRINKIIKDMQRHPFEGIAK
                     PEPLKYDYQGAWSRRIDAENRLIYMVEADRLCILSLKDHYR"
     gene            complement(14867..15127)
                     /locus_tag="AC1PNG_000310"
     CDS             complement(14867..15127)
                     /locus_tag="AC1PNG_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIP6700848.1"
                     /translation="MEAIVYSHFRNHLKDYMKKVNDEFEPLVVVNKNPEEDIVVLSKS
                     EWDSLQETLAVARNTYLSQKVLRGMAKVKTGQTQERNLIEAD"
     gene            complement(15203..15658)
                     /locus_tag="AC1PNG_000311"
     CDS             complement(15203..15658)
                     /locus_tag="AC1PNG_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HIP6700849.1"
                     /translation="MSRREQVVLTNMCMITDGRRVLVQDRKSEKWPGVTFPGGHVEHG
                     ESIVSSVVREVKEETGLTVSNLELCGIQNWTDPTDHYRYLVFCYKTSHFSGSIQSSDE
                     GEVFWSDRAELKNVQLADGFETMLEIFEQPQLTENFYWFEDGEWKVENR"
     gene            complement(15662..16030)
                     /locus_tag="AC1PNG_000312"
     CDS             complement(15662..16030)
                     /locus_tag="AC1PNG_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700850.1"
                     /translation="MDRVYAVKCLVKNEFEPTYENYSSYEEVTYLLPANVLVWDDEQV
                     DKNKLADYLRGLVEDGYVNADGIWKQSRLFHIAGVQEMDDRVEFDGPAVEVYRWFYNF
                     ERPVTEEEFLNLYYFDDLGR"
     gene            complement(16023..16592)
                     /gene="rnmV"
                     /locus_tag="AC1PNG_000313"
     CDS             complement(16023..16592)
                     /gene="rnmV"
                     /locus_tag="AC1PNG_000313"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HIP6700851.1"
                     /translation="MTQKIKIQEVLVVEGKDDTANLKRFYEVDTYETRGSAISDDDLE
                     RIEKLHDLRGVIVFTDPDYNGERIRKIIMQAIPTVKHAFLQRDEATPKSKTTGRSLGI
                     EHASFEDLEKALAGVRGYYDDENNFDISKSDLMRLGLLMGGESRKRREYLGEKLRIGY
                     CNGKQLLKRLELFGISLVEVEEAMESYHG"
     gene            complement(16576..17358)
                     /locus_tag="AC1PNG_000314"
     CDS             complement(16576..17358)
                     /locus_tag="AC1PNG_000314"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIP6700852.1"
                     /translation="MIQIFDTHTHLNVEQFDGRVQEELDFAKEMGVTLHNVVGFDRAT
                     IDKAMELADQHPEIYLTLGWHPTEAGTYDEEVENYLLKALQHPKVIALGEIGLDYYWM
                     TADKEIQARVFKRQMELAKQLDLPFVVHTRDALEDTYQIIKEVGVGPRGGIMHSFSGT
                     LEEAQAFMDLGMHISFSGVVTFKKAVELQEAAQALPLEKILVETDAPYLAPVPKRGRE
                     NRTGYTRYVVDMLAQLRNQSVEEVARATYENARSLFRIDSEN"
     gene            complement(17466..17603)
                     /gene="rpmH"
                     /locus_tag="AC1PNG_000315"
     CDS             complement(17466..17603)
                     /gene="rpmH"
                     /locus_tag="AC1PNG_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIP6700853.1"
                     /translation="MKRTFQPSKIRRARKHGFRSRMATKNGRRVLAARRRKGRKVLTV
                     A"
     gene            complement(17771..18766)
                     /gene="jag"
                     /locus_tag="AC1PNG_000316"
     CDS             complement(17771..18766)
                     /gene="jag"
                     /locus_tag="AC1PNG_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HIP6700854.1"
                     /translation="MKFTGASVEEAIQNGLVELNIPRKKAHITVVAREKKGFLGFGKK
                     PAIVDIDVINETTVVKANQKAVRGVPSEINDLNEPVKSVSEATIDLGKVVAAVKEFEK
                     SGQTLDDDIKAQILKNEKEATTILEETGRIEILSEDLLTTTDATTSAKEDVNQEVVSE
                     NQADNFADLDIKIEPQYDIEQVVSEVTAYVQEILDDMDVEARIDTSHNRRTINIQVDT
                     NEPGRVIGYHGKVLKALQLLAQNFLYNRYERNFYITINVNDYVEHRAEVLQGYAQKLA
                     ERVLAEQEAYHTDPMSSSERKIIHRIISKMDGLTSYSEGSEPNRYVVVDIEGNHD"
     gene            complement(18786..19598)
                     /locus_tag="AC1PNG_000317"
     CDS             complement(18786..19598)
                     /locus_tag="AC1PNG_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HIP6700855.1"
                     /translation="MKKKVKWAGLLVASLIVLTACGTGPVTSQSTDAWDRLIYWFASI
                     IQFLSINGQIGIGIILFTILIRTLLLPLFQIQMNSSRKMQELQPQLKKLQAEYPGTDM
                     ESRQQLYEATQALYKENGVSMRASMIPLFIQMPILLALFQALTRVEALKVGHFLWLNL
                     GEPDPFFILPIMAAVFTFLSSWLTNKSAIEKNMAMTIMTYVMPVMIFFFAVAAASGVA
                     LYWTVSNAYQVAQTLVFNNPFKIIAERQAKIDAEKERQAKIRRAQKKAQKKK"
     gene            complement(19582..19941)
                     /gene="rnpA"
                     /locus_tag="AC1PNG_000318"
     CDS             complement(19582..19941)
                     /gene="rnpA"
                     /locus_tag="AC1PNG_000318"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIP6700856.1"
                     /translation="MKKSYCVKRERDFNDIFTKGNNVANRRFVVYRLPKEQKHFRVGL
                     SVSKKLGNAVMRNKIKRRIRHILIECQHQLVADDFVIIARKGVEELSYQEMKQNLIHV
                     LKLAKLYQEGVVIEKES"
     gene            complement(20012..21406)
                     /gene="argH"
                     /locus_tag="AC1PNG_000319"
     CDS             complement(20012..21406)
                     /gene="argH"
                     /locus_tag="AC1PNG_000319"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIP6700857.1"
                     /translation="MEAKKLWGGRFEASLEEWVEEFGASIRFDQKLAKYDIQGSLAHV
                     KMLGQTGIVSQAEAQVIQAGLEELLEEYEAGKLVFDIRNEDIHMNIESLLTEKIGSVA
                     GKIHTARSRNDQVATDMHLYLKDTLFQVVDKLTNLRQILVDLAQEHVETIMPGYTHLQ
                     HAQPISFAQHLLAYYNMFSRDSERFAFNMQHTNVSPLGAAALAGTTFPIDRRMTSDLM
                     GFAKPYSNSLDAVSDRDFILEFLSNASILMMHMSRLCEEIINWCSHEYQFVTLSDTFS
                     TGSSIMPQKKNPDMAELIRGKTGRVYGNLVGLLTVMKSLPLTYNKDLQEDKEGMFDTA
                     ETVLISIDILAGMLQSMTVHKERMAQSTEKDFSNATELADYLASKGLPFRQAHEIVGK
                     LVLECSKAGHYLQDVSFETYQAISPLIQADIYDALSSKVAVSRRNSLGGTGFESIADQ
                     LKSAKEEIQNAKSL"
     gene            complement(21420..22613)
                     /locus_tag="AC1PNG_000320"
     CDS             complement(21420..22613)
                     /locus_tag="AC1PNG_000320"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIP6700858.1"
                     /translation="MTKEKLILAYSGGLDTSVAIAWLKKDYDVIAVCMDVGEGKDLDF
                     IHDKALSIGAIESHVLDVKEEFAQEYVLPALQAHAYYEQKYPLVSALSRPLISKKLVE
                     MAHKTGATTIAHGCTGKGNDQVRFEVAIAALDPSLKVVAPVREWKWAREEEIIFAKEN
                     GVPVPADLDSPYSVDQNLWGRANECGVLENPWNEAPEDAFGITTSPENAPDTPEYVDI
                     EFKAGIPVAVNGEKLSLANLIQRLNVIAGKHGVGRIDHVENRLVGIKSREIYECPGAI
                     TLLTAHKEIEDLTLVREVSHFKPIVSNELSNLIYNGLWFNPATDALKAYLAQTQAVVN
                     GTAKVKLYKGSAKVVARKSPNSLYDEDLATYTSADTFDQDAAVGFIKLWGLPTKVNAE
                     IHKKS"
BASE COUNT         6420 a         5313 c         4568 g         6394 t
ORIGIN      
        1 aattttatac tattttgact gcagagcaaa aaaattgaag gaagatgtcc gaaacaaacc
       61 ttcctccaat ctgagctgaa aattcagctt tttctttatg cacgaataat ctaaataggg
      121 accaccttac atctctttaa gtgcaggttg gtaaatgtta ttcaaaaata tttcccaact
      181 ctacatcgac tcggttttcc ttgacattga tgtcggtcac agcgagcaaa gacttgtagt
      241 tagctacgct acggtcccct ttttggtttt ctgcaaagac agccacgcct accaaggttg
      301 agtcaaactc agacaagagg ctaatcatac cattgatggt gccaccattt ttcaagaagt
      361 catcgacgat taagacacgg cttcctgcct tcaaactacg cttagacagg aacatttttt
      421 cgatacggtc gctagatcct gacacatagt tgacagaaac ggttgaacct tctgtgattt
      481 tcaaatcacg acgcacaatc acaaacggta cattaaggac attggcaaca gcattggcca
      541 aagggacccc cttagtcgca actgtcatga ccgcatcaat cttttcatct ttgaaagcat
      601 ctgcaatgat acgaccgaca tttttcaaaa tatgtggtgt agacagcaag tcagatgaat
      661 agatataacc gcctggcaag atacgattac tttcggccat ctgatcacga agggcctgtg
      721 caatttcgac agactctgcc ttagagatcg atggagtaaa aacaactcct ccactggcac
      781 cagtaatggt ttcaatatgc ccgatagagc tttcctcaaa agccttttta atgatggcaa
      841 tatcctctga gatagatgac ttggcagatt catacttttc cgcaaaggta ttcaaactag
      901 ttaattcata agggtggttg atgaggtaat tggaaatgac aaccatacgc tcacttcttc
      961 ttaatttcat aaaatcatcc tctttcactt ttttctatta tatcacaaca aactacaaaa
     1021 agcgaacttt tttgattgaa aagtccgtct ttttcgtatt ttgtttatcg cttacatata
     1081 ccactttgga agatgatgat taaaatattc ataccattca gaaatcttct gctcttgcaa
     1141 cttcatcaat ggaatttgtt cggaatctac ttgttctgct caagaaagag cgcctagact
     1201 attactgtta cataaagagc catgagtctc caaaattcct ctggaatagc gccgtcaaaa
     1261 taggagtcta cctgaccacg cgcaaaggct ggagacaaat ctgccgtgaa aatcaaacgg
     1321 ttgaattctt cccaggggtc tcctatgtcg tagcgatcaa agtctaatat tttcagtttc
     1381 ccatccgcac ccagtaggaa gttacctgtg tgaaaatctc catgatggta aggtctccct
     1441 tcaagcaagt gacgattggc ctgaacaaag tctatcatgg cttgaccatt tggataggaa
     1501 tgactagctg cttggtagac agctaattta ttgtcaatct tagcctgata aaaactgttc
     1561 cagtcgcgtt gactttggtc aatcggtagg gcatgcaagg ttcgcaaaaa ctgccctgac
     1621 tgacagccca aatcatataa gacagaatca gacaagctag tagccacttc attcatgtcc
     1681 tgaccttcca cccattcata gagtgtataa actgactcct cacccaccca aagagcgatt
     1741 ggctctgcca caggcaaacc tagaccaaac agatttttaa ctaactgaaa ctctaatcgc
     1801 ttagcctcat aggctggtcg ctctgctatt ctcaaaagac caaggcgacc atcttctagc
     1861 cggaccttgt actttcggtc cgtggaccag cctttggtga ggggctgtcg agacactatc
     1921 ctaactgcca ttagtcgatg ttgggcttat agaaagcgcg gttatccata gcaaagatgc
     1981 gattggtcat ctcgcctggg ccgaccttgt ccaaggccga tagcatcatc atcatttccg
     2041 catcaatgtt gtcaatcatg tggaggattt ctgcctccat aatctgcgga cggactgggc
     2101 ttccatactc caagaggcca tggtgactga ggataacgtg gcgcagaaca gtcacttcct
     2161 ccagactatc atcgatgccc aattccatca agaccttggt aatctcttca tcaatcaagg
     2221 aaatgtgacc aatcagattg ccacggacag tgtagccagt attgtctgga ccagtcagtt
     2281 cgatcacctt ggctaggtca tgaagcatga ttcctgcaaa gagcaggctc ttgttgagct
     2341 ggaggtagat gtcaccaatt ttatcagcca agcgtaccat ggtcgctgta tggaaggaca
     2401 agcctgagta gaaggcatgg tgattggtct tagctgctgg ataggaataa aattccttgt
     2461 cgtacttgct gtagagagca cggacaatcc gctgccatgt ggcattttcg atgcggaaaa
     2521 tcatctggct cagatagtcc ttggtgtcct tgacatccac aggtggtttt tccttgaaat
     2581 cagcagggtc gttgggctcg cccgcctttg gcaaacggag aacaagctgg ttgacctgag
     2641 gggtgttgtt gtaaacctca cggcgaccct gcatgtgaac aacagtgcca gcagtaaagt
     2701 ccttgatttt ccctggctgg gcatcccaga ccttgccttc aatctcgcca gtatcatctt
     2761 ggaaggtcag agctaggtag tctttgccag ctcgtgtttg acggacttca gcagtcttaa
     2821 tcagatagaa tccttcgaag aattcatctt ttttcatttg gttaattttc attatcttcc
     2881 tcatctaatg gggacaggcc aagcagtccc ttggtttggt catcttcgta caggtcaatg
     2941 gtacgaagac tacgctcgat ggcgttggta cgagtggtta ggagtttgtc aatattgctg
     3001 ctggcttgat tgagctgttt ctgggcctta agcaagaggt cagaaaactt gccaaattcc
     3061 agcttgacat tgcccaagac cttggaaata tcatcggctg accgttgaat gttaagggtc
     3121 ttaaagccga cggagaggga attgagcagg gcagacaggg tggtcggtcc agctactacg
     3181 atattttctt gacgacgaag ctcatcaaag aaaatcggat tgcggaccac ctctgaataa
     3241 agtccctcgg ttggcaaaaa caagacgcca aagttggtgg tttcaggcgg attgaggtac
     3301 ttgctctgaa tgtccttggc aaaacgcttg atagccgcca agagattttt gcggtggaga
     3361 tcaatctggt ccttatcccc actttcgtaa gcatcttcca agcgataata atccgccaac
     3421 ggaaacttgg agtcaatcgg cagatagata tagtctccct cggtccgtcc cggtagcttg
     3481 accgcatact ccacacgctc actagagcct gaaactgtgg caaactctcg ctcatattgg
     3541 ctaggtgtca aaatatcctc gataatctgt cccagctgta attcacccat gatgccacgg
     3601 gttttggtgc cagacagcac cttattaaga ctccccacat cacgcgccac cgtctgcatt
     3661 tcacccagac cacgattgac cgattccaac tgcttggaca ccgtttcaaa ggaggcctgt
     3721 aggcgggttt gcagggtttt ctccaacttt tcctcgactg tttgccgcat ctctttcaag
     3781 cgtttttcat ttgactcctg aatagcctgc aagcgacggt cggtggcatc tcgcgtttcc
     3841 aagtggcttc tattcagctc ttgacggatg tcggttagct gttggtaaag ctccgtgcgg
     3901 agtctttcca cctcctgatg aatggcctgt tgctgtttga gagttgcatt ttctagttga
     3961 tagctgagct gatcagacaa gttgtctgct gtatcttcgg cttgatcttg caaaagcagg
     4021 gccaagctct gccattttcc ataaagaaaa accaaggcaa gcaagaccag tatcagcaaa
     4081 ataagtagta caatatccat ctaattctta tccttactgt agatcaggac cacgtagccc
     4141 tgatccagtt gaatgtctat gtctctatct ataaattcgt tagaagcata gcattttttg
     4201 aaaaaataat tgctggcatc cagcgggtac ttggcatgac gaatggtcag gcgactttgg
     4261 tccgacggca taaaggaaat gtacttcata ccagcaatcg gtgacaaacg gtgctgacct
     4321 gcaggtcgaa atcggacgat gttctgacta tccaccaatt caatctgctc catgtagggt
     4381 gccaagtcgg gttctgcaac cagaaagaga ttggccatca tatgatccat gcgaccaccc
     4441 aaggctccgt atatgcgaac ctgggcctgc ggataaacct tgaagacttc ctttaacgct
     4501 aactctaagt ctgtatcatc tttttcagca ggagcttgca aaaaacgctc tgcttgatcc
     4561 tttatccgcc ccaactcctc cgaggtcacc gagtcaaaat caccgatagc ccagtcaaga
     4621 ggcaggaaat ggtccagaag acgaagagag cctgcatcta cccccacata gaggtcggca
     4681 ggctcaggaa agcagtcaaa ggaaccgcct gcaatgacag caatcttaat catggagggc
     4741 agctctcaat ttggcaacgt tggcatccaa gtctcctttg aagaggtagg atccagccac
     4801 aaagacattg gcacccgcat ttttggctga atgaatggtc ttatcgtcaa tcccgccgtc
     4861 cacttcgatg tcaaagttca agcctttagc ttcacgcaat ttaaccaaat ctgcaatctt
     4921 ctcagccact tctggaatgt aggcttgtcc accaaaacct gggttgaccg tcatgagcag
     4981 aacctgatcc accaagttga gcacaggctc aatggtcaca agaggagtgc ctggattgat
     5041 caccacaccc accttcatac cagcagcacg aattttttgg agggtcccat gcaagtgaac
     5101 cgttgcttct gcgtgaatag tcaagatatc cgcacccgca cgtgcaaagg tctcgatatg
     5161 attttcagga ttggacacca tgagatggca atcaaaaacc agcttgctat gaggacgcat
     5221 agctgctacc acatccgccc caaagctaat atttggcaca aagtgaccgt ccatgacatc
     5281 aatgtgcaca tattccacac ctgtcttttc tagacgcttg agctcttttt caaaatttgc
     5341 ataatctgcc gccaaaatag acggtgcaat cttataatgt gacatacaga accaactttc
     5401 tatttgaatt tcttacttac cttggtatag gtttcacgct gattttggat ttcgctgagg
     5461 aattggagat aattgtcaaa gcgactttgg gaaatggcag agctggcaac agcttccttg
     5521 accgcacaat ccggctcatg ggtatgggta caggtcctaa atttgcagtc ctgactgctt
     5581 tccgcaatct ctgggaaaca atctgtcaga gcctccgcct ccttgacttc atagtctaga
     5641 gatgaaaaac ctggcgtatc cgcaattttc ccaccgaaga cattgtagaa actgaccgca
     5701 cgggtggtat ggcgaccacg ccccagacta tctgaaatag cacctgtttc caaggctaat
     5761 tctggcgcaa tgcgattgag aagtgtcgat ttgccaaccc ctgtctgccc cataaagacc
     5821 gtcaccttat cctgcaataa tggcgtcaat tcctccaaac tatagacaaa aggatagcta
     5881 attttctcat agatagcctt gaaggcatcc atctccgtcc gatcttctac caaatccatc
     5941 ttagagatat agataatggg atccatatcc ttctgttcaa ggagaactag gaaacgatcc
     6001 agtagattgg cattaaaatc aggttccttg gccgacatga taaccaccgc ctgatcgata
     6061 ttgacgatag gaggtcgaac caaactattt ttccgctcat gaatctttaa tatatagcct
     6121 tctgagtttt cctccgcaga aaagtccaca aaatcaccca cgtagggcgt ttgtcccttc
     6181 ttacgaaaat ttcctctggc tctggtttga taaatctgtc catccgcctc gacatagtaa
     6241 aaacctgcca aggccttgat aattcttcct tgcaatgaag actcctttgt gtgtacttgc
     6301 tactattata ccaaatttcc tacaaaaaaa ccgcaagcgg tttcgatttt acattttcct
     6361 ctagtcattt caatgtaaaa atagtataat aaaaagcaag cggaggtggt ggaacggcag
     6421 acacgcatgc ttcaggcgca tgtgcccatc gggtgtgagg gttcaaatcc cttcctccgc
     6481 attatttata aaggataact ccaagtcaaa gttgatgact tgggggcatt tttatatctt
     6541 ctcgctcata ctcaatgaaa atcaaaaaca gactagctcc aaaggttttg gaaacctttg
     6601 gaggttggaa atagagcgaa cgtagttcgt ttctacttac gcagatagaa ccctgttact
     6661 attttgtttc aaggtaacag gctgaaaggc tccactggag cctttcactc atcaaatcaa
     6721 gtcaacaatg tctgattttg attttcgaag aatattaatt gctctcccca atccgtacca
     6781 ccccatcata acggctaagg acctcctcgg acatgcggtg ggaaatatga atgagcgtct
     6841 tatcagctag tgaggtaagg taattttcaa tcactgcata ggtttcctga tccaaggcag
     6901 acagactttc atccagcaac aagatatcct tatcttgcag caaggcacgg atcaagagca
     6961 ggcgttgctt ttgtccgcca gacagtccgc tatcatcact aatttcctcg ctcaaagaag
     7021 cataacttgg gaaacgactg tcaaggttca tggtcttaat gagaaacatc agcctatctt
     7081 ctggaatctc ccgtcccatg gtcaagttgt atagaaccgt atcatggaaa agggatccct
     7141 cttgtggcag ataggcaatc ttgtcttgaa tggcttggta ggataagtct gaaagagagg
     7201 ttttatcaac gtaaatatcc cctgcttgca gttggctatt cttggtcaaa ataagagcca
     7261 aggaagattt gccagaacca ctttccccaa gaattaaata cttcttgcca cgctcaaacg
     7321 ctacggtcag gtcttcaaag acctgttttt caccgacagc ataactagcc ttgaccagtt
     7381 gaatactctg gaaagctgaa gctagttcct gtcccttcgt ttcttgacca aaagtagata
     7441 gctcaaactc ttgctttaat ttcttaacgg catgcctgtc caaaaaggct gccgcaatgc
     7501 tattaatggg atccaccaac tgctctgaaa tggtcagaat agctgtcaag ccaccgatgg
     7561 tcaaggcacc agaaaagact tgaaaaccac cgataaagat aatcgaaaga gtggtcagat
     7621 aaaagacaaa accgactaag agccgcatgg ttactgtcgt tcgagtggcc tgattgacct
     7681 tgtcattgac tgcttgatag cgatcgttca gaccatctaa aacttgctgt ctttggtggc
     7741 tatttttcac ttgcaaataa ctcagaaaga catctgaaac tcttgtatta tagacctgct
     7801 gactgtccga aatcgcttct tgattgagcc ttctccgctt tttaaacaaa tagggaatca
     7861 caaccggcaa gaatgagatc agaatcaaga aaatagcggt cagaccatcc aagctaagca
     7921 aggccaaact ggcaatgcca aaggtacaag caccttgaaa aatcgtaaag aaagcgtcgt
     7981 aataatcttc tttcaccgtc tccaaatcat tattgagctg ggaaatatag gcccctgccc
     8041 cctgctcaac aaactgccta aaaggtaaat caaaaatttt cccaataaaa gtcgcctgca
     8101 attggaaacc tacttgattg agatagctga cattggcaat ctgacgaatg tattcaaaga
     8161 ccagcatgac aaggacagtt agtcccatca gcacaaaata ggacacaaag gcctgtctat
     8221 ctccagctgc aatagcatca ataatatagc gatattggta cagaaagcca acattgagca
     8281 gggctactgc aaaaatacaa gttaaggtta gccaaaaatt cctctttttc atttctcctc
     8341 cactgtttga tagatttcta agcaaagcaa taaaaaaatc tgcattaaac acctttatta
     8401 taccactcct tcccatattt gtgtccattt tcacataaag aaaaagaagc taggcataaa
     8461 gtccagcctc gcttctcaga gttcgtgtca acatctcagc gcagtggttg attggcagat
     8521 ttggtcgtgt tgcacactcc aaatctgacc taatcaactg tgcgggggtg ggaagacgaa
     8581 ctctttattg accagtcgag ttctttccca ctcccctatt tttattctgt cgtttcagtc
     8641 gctactttga aaaaatcttt gtatttcaaa aggtagcctt ttcctgtgga taggtcatat
     8701 tggaccagtt tgagttcttc tgcaactttc gcatctcgct cagcctgtcc tactcggtcc
     8761 ttatgaagta cttctgtcaa gagggcatag taaggactga ccttgttatt ggtcgtctcc
     8821 agcatcagtg cattcatgtc gctagaattg accaaatcat aatggtagtc ttctgtttca
     8881 aaattactcc acacgaaata atccgtcttg tactgcgcac ttgggtccgt cacgaaagct
     8941 gactctggat acaaacctgg taggtggtca ccataaaaaa caacggtcac tttcttatcc
     9001 aaggtcttga gctggtctag aaaatccctg gttgcctgat ccgtaaagga tagaagacga
     9061 acatagctat tgaggttggc attctcctca gccgtgaaac cctcacccga agctgtaata
     9121 cttgcaggct cagcagagtt ccactgaaca tggttctgca tggtaatggc cgagacaaat
     9181 gtatttgttc ctgaacgtac ctgttccaaa atcaaatcat aggtcttttt atcactgaca
     9241 aaattcccct gataatcttc agccgtcact gcaagtggat acttggcact attgagcgta
     9301 tagaactgct caatccccaa ctgcttatag atagaattac gattgtaact ggtatcgaag
     9361 taggggtgaa tagcgacacg attctctgct ggatagaggt cactgatact tggcaacttc
     9421 ttcatatttg gcgccacatc caaatagact gaagaaatgg atgagctgta attgtagaag
     9481 ggcagaccac tatagacctg aaactcgata ttagccgtac caccaccata gtgatcactc
     9541 ttcatcaagc cactggtagt ctgactcata atgtactcaa tatttggaag gacattttga
     9601 ctaagggtta caccgggaat ccgacgcggg tcagccaaac tctcactaag aatgtagata
     9661 accgtctggt cagtcaattg acctgtccgt tctgcattga tttcctcagc cagagcggta
     9721 tacttcttca caatggcctt catggtcttc tcagagtaat tctcaggcat ttccatactg
     9781 gtcttgctga gttgctgcaa ccagaggaat gaaagagtgc gataacgagc cacaaaggcg
     9841 tagcccttcc agtctacgtt ccgccagtta ttgacctgag aaagaatagg aatccagcca
     9901 gcaatcttgt ggtctttttc atttcgaata gcccagccca ttcccaaaat tagggatacg
     9961 atgactgaaa ttcctgccag tcgtttccag atagcagcga caatctgccc tcggaaatac
    10021 ttcttgtgaa tatgtcggta gataaacaca aagaggacta taaagacgag cgataccaaa
    10081 ataacacgta gactgataaa gctgagaatc atccccaagt tcccgaccca tttgaaatca
    10141 ttgggggtca atggctccga acggtagcgg aatttgagaa aattggccgt aaccaaaatg
    10201 acgtttagac tagcaatcac ggcaacagat aagatttggc ggttgataac taggctaatg
    10261 atgacattga ggaaaaagag gcaggcaatc tggaatacaa tagcccctgg aaagagatga
    10321 cgtgtgaaat aagaaccagt cgctccagcc atagaagcct gataaccaat atgcgatagc
    10381 gtagccagtg ccaaactgat aaagagcatg ctgtggacat tggttcgatt ggccttaaac
    10441 tctacaaatg ccttatgaag atagcgaaga aaaatgtagg tccctataaa atgaataggt
    10501 gcaaacagcg gtgtctggct gaaaaactct atgtaagaac ctttctcaac caaactctca
    10561 taatggctat ctttccagta agatatggct agaggactga gcgagagtaa gctggtcaag
    10621 agcaaatagc catctggtac caagtgattg gctgaccact ggcttaattt ttctcgaatt
    10681 cccgtcttct tccgccataa aaatgccaac aatacaggaa gagccaacat caatagaagc
    10741 tcaaattgaa agagattatt ccggaaaata ttccagggtt gatacttctt ggcattgagc
    10801 cgatagacca ggttgacaag atatagaaaa cctagaaaat agctgacata cttggcaaag
    10861 gttttggtct ttataaattt ctcatattta tcaagcaaga ccaatacata aaatgtaaaa
    10921 ataagatagg caatcaaggg taggggaata aaccagagtg gaaaagatac atcactcagt
    10981 aaaagggctc gccccatagc cagcaagacg gtcattacca tgaccagaat aaattgcttg
    11041 gttgccttga gtttgatttg tttcatagca tttctctctt aggaaatatt tgattttcgg
    11101 tagataaagg tataataaat cgaccaaagt atccagccag tcaaactgat aaagataact
    11161 ggcaataacc accattgttg cttataggag aggattaggg tgttcttccc tttttggctg
    11221 gtaaccgttg gcacaccaat agtggaaaga taataatcca tcttccctaa cctctttcca
    11281 ttcaatgtca attcactatc tcggtaaata acaatcggta tttggatttc ctctgattga
    11341 tttgctgacc aacttatttt taagctatcc ccaaaaactt gcttattaaa ttgctggtta
    11401 ggtaaaataa tctggtcacg gtaacgatca taacgagatt tagcctcatt taccgcacga
    11461 acattctctt ctttttcaga taacaccggc acatagtcgg gagttgattt ctgagccagt
    11521 tgaacaaact cacctaaatt ttttgaatgc aaggatgcac gtaattcttc cgttgttccg
    11581 tatagagtag tatgaagcat atcttgtaag aattctgctt tgttgtactg gtcattgact
    11641 gcttgattat attgatccca agtaaaacca acaccaatta tggtagataa gattgtcaac
    11701 cccaaaaaca actttttacg tacttcgtaa tctgttaatt gaagaccaac aatcgtcaga
    11761 agtaaagcat tagcatacaa aaagaatcta aagggaaatt ggattaaatc cactaatcca
    11821 atacctttac cagcaaatag ttgccacgga aaaagatttg ttgaaaaaat aaaaaatacc
    11881 agaaaagtta ctgtcagccc cctcaatatc accgattgct tcttccattt tttcagcata
    11941 aaataaagaa aggttgcaaa aatgattggt agagagtatg gaaagtataa gaaataagcc
    12001 ttagaccagg taactgttga ctgaataaac tttttgttta taaatggtgc tgctaattca
    12061 tttgttgcat ttacatataa taagggtaac cagatattta cagttaaaat cagaaaaact
    12121 ccaaccgaaa acagtacatc tttcattacg ttccatttgt cttgcgattt taaccaacca
    12181 attgaaaaaa gaattgcata ggctaagatg agaaacagag ccgacagcat gtgtatctgc
    12241 aacattaacc ctacagctcc tgccatcaga aaaataggaa ctttaccagt ttttaggaat
    12301 cgaactgctg gaatcaagca aagcggaaat aaggccactc cccaacttga aaatccttga
    12361 gctacccacc agtattgaac agagtaggta gtcacgaaaa acatggataa cccaacagat
    12421 agagaatagc gtactttcac ttctcgcatt agaaaataaa gcgaagtggc actcaaaact
    12481 cctaaaagaa gattagacag tagttgatat cgataccaag ttccggccaa caacagcaag
    12541 gctccttgca aataggctag atatggacca tatagagcat tgacaatcct tccagattgc
    12601 tgatagccat gcaagctcat tatataagaa aaatctcctg acttaatttg catagctgta
    12661 tcataaaaac gattataatg aaaaacaata tcgtctcctg tcaccactgc ttttgacaaa
    12721 atctgtggca atagaagggt gagcgaaaaa agaaaaaata cagttactgc aacaatatct
    12781 ggatgctttt tcagtaattt ttttaacata agataccttc ataaaaaaag agggaacccc
    12841 tctcttgttt ttgtaagaac ctgtactcag aagtgaagtg attatcaata agagatagtt
    12901 tttcgctaat caaggcagtt ttattgagag aatactgact gtactttgaa aaaactaacg
    12961 atgactagct taaaaactat ccttaagtag aagtgctgaa ctgtgaatac gggttcttac
    13021 tattctgctc atttgcagac aataattcct taaaatttat caactcccgg tataacaagc
    13081 tttcccccca ctgtcaacgg acaagataga ttgaaaatgg ttttaggtaa aataaagatt
    13141 gattgcagat ttctccgcaa tcaatacttg cttataaacc aacttcctga aggctatcag
    13201 acaaacgtgc aaagtcagcc atggaaagtg cttctccacg gacactggct tccagctctg
    13261 ccatgccaag tgcccgcgtc agcttgtcct tgacctcgtc ggacttgcca aaatggttgg
    13321 tcaggttgtt ccacagggtc ttacggcggt gcacaaagct ggccttggac actcggaaga
    13381 agaaatcttc atctttgacg ctcaccagag gttgctcgcg gcgaaccatt ttgagaatgg
    13441 cggagtccac gtttggagcg ggcacaaaga cggtgcgagg cacgatgaaa gccaccttgg
    13501 cagtcatgta gtactggacc gcaatggaca ggctgccata ggccttggtg tttggctcag
    13561 cagaaatgcg gtcggccacc tcccgctgca tcatgaccac aaactcgctg aagggaatct
    13621 tgctctcaat caggtgcatg aggatagggg tggtgatgta gtagggcagg ttggccacga
    13681 ccttgatagg cagatctgga ttcttgaagt tctgaatctg ggtctgcagg tcggtcttga
    13741 ggatatcttg gttgaccaca gtcacattgt caaagcgacc caaggtgtcc gccaaaatcg
    13801 gaatcaggcg gtcgtcaatc tcaaaggcca tgacctcggc ggcattctcc gccaaaaact
    13861 cagtcaaggc tccaattcca ggaccgattt cgatgacatt gacattctgg tcaatctctg
    13921 ccgtgttcac aatcttctgt aaaatattag tatcggtcaa aaagttctga ccaaaggatt
    13981 tcttaaaggt aaacccgtgg cgttccagga cggcacgagt aacgtttttg tctgcgattc
    14041 tcatgtgtgt tctttcttgt tttttatttc gccatttcac tcaatcgtga aataacactt
    14101 agttttctca tcgtcgctgt aatgtccagc tgtttttcta aataattggt atttagtttt
    14161 ttctttcgag catctctcgg aagatacatg tatagacatt gacttcccag acgcaactcc
    14221 tcatcgccaa agtcttggtg caataatttg tttaattttt caccatttat ctcatcattg
    14281 gtaaagacca aatgaacacg agaaggatca tagccctcac caaaagaatt ttcagcaact
    14341 gcttgtgtta actccatctg atgtttgaca atgatcgcta actctgcacc tattttttct
    14401 ttaatggttt gatggataag ccgactgagt tcttcatcta atatatctgt ttcacaaaca
    14461 acatttccgc tttgaatata ggtttggaca gaagttaaac cagcttccgt caaaatttcc
    14521 gccaagtaag acatcttggg aattttattc ttgccaacag gggtaacacc tcgaaggagg
    14581 acgacttttt tcatttgtta atccccctca cctataatga tcctttagcg acaggatgca
    14641 caaccgatca gcttccacca tataaatcaa ccgattctcc gcatcaattc tccgagacca
    14701 ggcaccttga taatcatact tcaaaggttc tggtttggca atgccttcaa aaggatgtcg
    14761 ctgcatatcc ttgataatct tattgattct cttcagtgtt ttcttatcct gaccctgcca
    14821 ataaagatag tcttcccagg cgtcgtctgt aaaatggatt cccatgttag tccgcctcta
    14881 tcaggtttcg ttcttgggtt tgtcccgttt tgactttagc cataccacgt aggacttttt
    14941 gagacagata agtgttccga gcaacagcga gggtttcttg taaactatcc cactcactct
    15001 ttgagagtac aacaatatct tcctctggat ttttattaac cacgaccaaa ggttcaaact
    15061 cgtcattgac ctttttcata tagtccttta agtgatttcg aaaatgggaa tatacaatag
    15121 cttccataag caatacctct catcatttct aggcttacta tagcacagaa aaaaagagtt
    15181 gtcaaactta ttgtacaact ctttagcgat tctccacctt ccactcgccg tcttcaaacc
    15241 agtaaaaatt ctccgtcagc tgtggctgtt caaagatttc cagcatggtt tcaaagccgt
    15301 cagccagttg aacattcttc aattctgcac ggtcactcca gaagacctcc ccctcatctg
    15361 aagattgaat agaacctgaa aaatgactag ttttatagca aaataccaga taacgatagt
    15421 ggtcagtcgg atccgtccag ttttgaatgc cacagagttc gagattggat accgtcaagc
    15481 ctgtttcttc tttgacctcg cggacgacag aggagacaat actctccccg tgctcaacat
    15541 ggcccccagg gaaagtcaca ccaggccact tttcagactt cctatcctgg accaacaccc
    15601 tccgaccgtc tgtaatcatg cacatattgg ttaagacaac ttgttctcgt ctagacattc
    15661 cctatctccc caaatcatca aaataataga gattcaaaaa ttcttcctca gtaactggac
    15721 gttcaaaatt gtaaaaccag cgatagactt ccacggctgg tccatcaaac tctaccctgt
    15781 cgtccatttc ttgcacaccg gcaatatgaa acaaacggct ctgcttccaa ataccatccg
    15841 cattgacata gccatcttca actaaaccac gcaaatagtc tgctagtttg ttcttatcaa
    15901 cctgctcatc atcccaaaca agaacattgg ccggcagcaa gtaggtcact tcttcataag
    15961 atgagtaatt ctcataagta ggctcaaact cgtttttaac caagcactta acagcgtaga
    16021 ccctatccat gatagctctc catagcctcc tcaacctcca ccaaacttat cccaaacaat
    16081 tccaatcgtt tgaggagttg tttgccgttg cagtagccga tgcggagctt ctctcccaga
    16141 tactccctcc gcttgcggct ttcacccccc atcagcaatc ctaggcgcat tagatctgac
    16201 ttgctgatgt caaagttatt ttcatcatca tagtagccac gcacgcctgc gagggccttt
    16261 tccaagtcct caaagctggc gtgctcaatg cccaaggacc gccctgttgt tttggactta
    16321 ggtgtagcct cgtcccgctg taagaaggcg tgtttgaccg tgggaatggc ctgcataatg
    16381 atttttcgaa tccgctcgcc attgtagtcg gggtcagtaa agacaatgac gccccgcagg
    16441 tcatggagtt tctcgatgcg ttccaagtca tcgtctgata tggcagagcc tctggtttcg
    16501 taggtatcca cctcgtaaaa tcgttttagg tttgctgtat catccttgcc ctcgacaacg
    16561 aggacttctt gtatcttaat tttctgagtc aattctgaac aagctccttg cattctcgta
    16621 ggtcgctctc gccacctctt cgaccgactg gttgcgaagc tgggccagca tatccaccac
    16681 atatctggtg tagccggtgc gattttcccg accacgcttg ggaactgggg ccaggtaggg
    16741 cgcatccgtc tcgaccagga ttttttctag gggcaatgct tgcgccgctt cctgaagttc
    16801 aacggctttt ttgaaggtca ccactcccga gaaggagatg tgcatgccca ggtccatgaa
    16861 agcttgagcc tcctccagag tgccagagaa ggaatgcatg atgcctccac gcggaccgac
    16921 gccgacttcc ttgatgatct ggtaagtatc ttccaaagca tcgcgagtat ggaccacaaa
    16981 gggcaaatcc aactgcttgg ccagctccat ctgtcgcttg aaaacacgag cctggatttc
    17041 cttatccgcc gtcatccagt agtaatccag tccaatctct cccagggcaa tgaccttggg
    17101 atgctggagg gctttgagca gatagttttc cacttcctcg tcataagtgc cagcctctgt
    17161 cggatgccag cccaatgtca gataaatttc agggtgctgg tcagcaagct ccatagcctt
    17221 gtcaatggtt gcacggtcaa aaccgaccac attgtggagg gtgaccccca tctccttggc
    17281 aaagtctaat tcttcttgga cccgaccgtc aaactgctcg acgttgaggt gggtatgggt
    17341 atcaaaaatt tgtatcatcg ttccatcact ttctttccta ctattgtaac atatttcaga
    17401 agattcttat gagaagaaaa aaaaccagca gactcgagtt tctgctggct tctcttttca
    17461 tcttcttatg ctactgtaag aactttacgt cccttacggc gacgagcagc taacacgcga
    17521 cggccgtttt tagttgccat acggctacgg aaaccgtgtt tacgcgcgcg acggatttta
    17581 cttggttgaa aagtacgttt cacgatgaat acctcctctt agattcttgt attcgtttag
    17641 ccggctagtt ttgatcagtt actaaacata ccaaactatt ttatataaat tctgttcgtt
    17701 tgtcaagcta ttctgccata cttttaggaa agaatcagaa aatcattgaa aaaagaggac
    17761 tagcctcttt ttaatcatga tttccttcaa tatctacaac gacatagcgg tttggttcgc
    17821 taccttctga atagctcgtc aaaccatcca ttttagagat aatgcggtgg ataattttac
    17881 gttcgctact tgacatcgga tctgtatggt aggcttcctg ctctgccaaa acacgttctg
    17941 ccaatttttg tgcgtagcct tgaagaacct ctgcgcggtg ttctacataa tcattcacat
    18001 tgattgtaat atagaaatta cgctcataac ggttgtataa gaagttctga gccaaaagtt
    18061 gcaaagcctt taagactttt ccgtgataac cgataacacg acctggttca ttggtatcca
    18121 cttgaatatt gattgtacgg cgattatgac ttgtatcaat acgcgcctca acatccatat
    18181 catccagaat ttcttgtaca taagctgtca cttctgaaac aacttgttca atatcatact
    18241 ggggctcaat cttaatatct aaatcagcaa aattatctgc ttgattttct gagacgactt
    18301 cttgatttac gtcttcctta gcagatgttg ttgcatctgt agtagttagt aaatcttctg
    18361 aaagaatttc aatccgacct gtttcttcca aaatcgttgt cgcttctttt tcatttttca
    18421 agatctgcgc tttaatatca tcatcaagcg tttgaccaga cttttcaaat tcttttaccg
    18481 ctgcaacaac ttttcctagg tcaatcgtag cctctgaaac gctttttact ggttcattga
    18541 ggtcattaat ttcacttgga acgccacgaa ctgctttctg attagcctta acaaccgttg
    18601 tttcattgat gacatcaata tccacaatcg ctggcttttt accgaaacca aggaaacctt
    18661 ttttctcacg cgcaaccaca gtaatatgtg ctttttttcg aggaatattt agctctacca
    18721 agccattctg gatggcctct tcaacacttg ctcctgtaaa tttcatcttc tctctcctag
    18781 ttttcctatt tctttttctg tgcttttttc tgtgcccgtc ggatttttgc ctgacgttct
    18841 ttttccgcat caattttcgc ttgacgctct gctattattt taaacggatt attgaacacc
    18901 aaggtttgag caacctgata ggcatttgac actgtccaat acaaagccac cccactagca
    18961 gctgccacag caaagaaaaa gatcatcacg ggcatgacat aggtcataat tgtcatagcc
    19021 atattcttct caattgctga tttattggtc aaccatgagc tgaggaatgt aaacactgca
    19081 gccataatcg gcaaaataaa gaacggatcc ggctccccaa gatttaacca aaggaagtga
    19141 cctactttca aggcttcaac acgtgtcaga gcttgaaaga gggccaaaag gattggcatt
    19201 tgaataaaga gcggaatcat ggacgctctc atgctgacgc cattctcttt gtagagagct
    19261 tgcgttgctt cataaagctg ctggcgactt tccatatccg ttcctggata ctccgcttgc
    19321 aactttttca gctgaggttg aagctcctgc atcttccgtg aggaattcat ctgtatttgg
    19381 aacaaaggca acagaagggt tcgaattaaa atggtaaaaa gtataatacc gattccaatt
    19441 tgaccgttga tagataggaa ctgaataatg ctggcaaacc agtaaattaa tctatcccac
    19501 gcatctgtag attgactggt caccggccct gttccgcagg ctgttaaaac aattagagat
    19561 gctaccagta gacctgccca tttaactttc tttttcaatc acaacacctt cctggtatag
    19621 tttagctagt ttcaaaacat ggatgaggtt ttgcttcatt tcctgatagg acaattcctc
    19681 cacacctttt cgagcaatga taacaaagtc atctgctacc agttgatgtt ggcattcaat
    19741 taagatgtga cgaatcctgc gcttaatctt atttcgcatg acagcgtttc ccaatttttt
    19801 gctgacagat aaacctactc ggaaatgctt ttgctcttta ggtaatcggt agacaacaaa
    19861 cctccgattg gcgacattgt ttccttttgt aaaaatgtca ttaaaatctc gttcacgctt
    19921 gacacaatag ctttttttca aactgccact ccatctttca aaattacttc tattatacca
    19981 caaaattcaa aaaagccaat aaacataccg tttataggct ttttgcgttc tgaatttcct
    20041 ctttggcact tttcaactgg tctgcaatcg attcaaaacc tgtcccgcca agggaatttc
    20101 tacgactaac agcaactttg gaggacaagg catcataaat atctgcttga ataagaggcg
    20161 aaatagcctg ataggtctca aatgatacat cctggagata atgtcctgcc ttgctacact
    20221 ccaagaccag ttttccaaca atctcatgcg cttggcggaa gggcagacct ttactagcca
    20281 aataatcagc caactctgta gcatttgaaa agtccttctc tgtcgattga gccatccttt
    20341 ccttatggac cgtcatagat tgcaacatcc ctgctaaaat atcaattgaa atcaaaactg
    20401 tttctgccgt gtcaaacatg ccttccttgt cttcctgcaa gtccttatta taggttaaag
    20461 gaagggattt catgactgtc agtaggccaa ctaagttccc ataaactcta cctgttttcc
    20521 cccgaatcag ctcagccata tctggatttt tcttctgagg catgatggaa gaacctgttg
    20581 aaaaggtatc ggatagggtg acaaattggt attcatgtga acaccagtta atgatttctt
    20641 cacacaaacg cgacatgtgc atcatgagga tactggcatt tgacagaaat tccaagataa
    20701 aatcacggtc agacacagca tcaagagaat tactataggg cttggcaaat cccatcaaat
    20761 cagacgtcat ccgacggtca atcggaaagg tcgtccctgc caaggccgcc gcaccaagtg
    20821 gggatacatt tgtatgctgc atgttgaatg caaagcgctc actgtctcgg ctgaacatgt
    20881 tgtaataggc taacaagtgc tgagcaaaac taatgggctg ggcatgttgc aagtgggtat
    20941 agccgggcat gattgtttcc acatgctcct gtgcaaggtc taccaaaatc tgacgtaggt
    21001 tggtcaattt gtctacaact tgaaagaggg tatccttcag atagaggtgc atatccgtag
    21061 ccacttggtc gttacgagaa cgagctgtat ggattttgcc tgcaactgag cctattttct
    21121 ccgtcaatag actttcaata ttcatgtgaa tgtcttcgtt tcgaatatca aataccagct
    21181 tgccagcctc atactcctct agcaattctt ccagaccagc ttgaatgacc tgagcctcag
    21241 cttgagagac aatcccagtt tgccccagca tcttgacatg ggccagtgag ccttgaatat
    21301 catacttggc caatttttgg tcaaagcgaa tggaggctcc gaattcttca acccattcct
    21361 caagactggc ttcaaagcga cctccccata atttctttgc ttccataagc tcctactttc
    21421 tagctctttt tatgaatttc cgcattgacc ttggttggta atccccaaag cttgatgaaa
    21481 ccaactgctg catcttgatc aaaggtatct gcactggtat aggttgccaa atcttcgtca
    21541 taaagggaat ttggagattt ccgagcaacc actttggctg accccttata caattttact
    21601 tttgcggtgc cattaacaac tgcctgggtc tgagcaagat aggccttcaa ggcatctgtc
    21661 gcagggttga accacaggcc attgtaaatc aagttagaga gttcatttga aacaattggt
    21721 ttgaaatgcg atacttcccg cacaagggtc aaatcttcga tttccttgtg ggctgtcaga
    21781 agggtaatag cacctggaca ctcataaatt tcccgtgact tgattccgac taaacgattt
    21841 tccacatggt caatccgacc cacaccatgt ttaccagcaa tgacattgag tctttgaatg
    21901 agattagcaa gacttaactt ttcaccattt acagcaacag gaataccggc tttaaactca
    21961 atatcgacat actcaggagt atctggtgca ttttctggag aagtagtgat gccaaaagca
    22021 tcttctggcg cttcattcca tggattttct aaaactccgc actcattggc ccgtccccaa
    22081 aggttttggt cgactgagta aggactgtcc aggtcagctg ggactggtac accattttcc
    22141 ttggcgaaga tgatttcttc ttcacgcgcc cacttccatt cacggacagg agccacgact
    22201 ttcaagcttg gatctagggc tgcaatggct acctcaaacc gcacctggtc atttccctta
    22261 ccagtacagc cgtgggcgat agtggttgct cctgtcttat gagccatttc caccaacttt
    22321 ttagaaatca agggacggct gagagccgat accagcggat atttttgctc atagtaggca
    22381 tgggcttgta gggctggtag gacatattct tgagcgaatt cttcttttac atccaataca
    22441 tgggactcaa tagcaccgat tgataaggcc ttatcatgta tgaagtcgag atccttgccc
    22501 tctccaacat ccatacaaac cgcaatgaca tcatagtctt tcttcaacca agcaatcgca
    22561 actgaagtat caagtccacc agaataggct aaaatcaatt tttcttttgt cataagtgtt
    22621 ctcatttcta tatatattaa caaataaaac ttagtgacta ttatagtgta tatttatacc
    22681 ttttgcaaca tattt
//
LOCUS       DBNFEG010000012        22490 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.012, whole genome shotgun sequence.
ACCESSION   DBNFEG010000012 DBNFEG010000000
VERSION     DBNFEG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22490
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.012"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(11..553)
                     /locus_tag="AC1PNG_000321"
     CDS             complement(11..553)
                     /locus_tag="AC1PNG_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700859.1"
                     /translation="MFLFAFPGMGKTTLAKKYTDVVDLEMSDIKYDNSSVRHLTKEER
                     KSTKRTLKDKGYKTIYVNKAYTLHRQGKTVLVALNFLMRMLLAMSIRGGVPFHIYIPH
                     PSLKEEYRQRYISRGNNDRFIFEVMFIWYPALIPLYLLSKILPKWITVTQSGETLEDY
                     YKRENFVRFSQKLRFIQHIS"
     gene            complement(693..782)
                     /gene="ffs"
                     /locus_tag="AC1PNG_000322"
     ncRNA           complement(693..782)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC1PNG_000322"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(825..1346)
                     /gene="tadA"
                     /locus_tag="AC1PNG_000323"
     CDS             complement(825..1346)
                     /gene="tadA"
                     /locus_tag="AC1PNG_000323"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIP6700860.1"
                     /translation="MNYTHEEKEYFMTQALQEARKSLEKDEIPIGCVIVKDGQIIGRG
                     HNAREELNQAIMHAEVMAIQEANNVEGNWRLLDSTLFVTIEPCVMCSGAIGLARIPQV
                     IYGATNKKFGAAGSLYDILTDERLNHRVKVETGILEAECANIMQDFFRQRRERQKAAK
                     LIMKTQEEATSDS"
     gene            complement(1626..3596)
                     /gene="tkt"
                     /locus_tag="AC1PNG_000324"
     CDS             complement(1626..3596)
                     /gene="tkt"
                     /locus_tag="AC1PNG_000324"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIP6700861.1"
                     /translation="MSNLSVNAIRFLGIDAIEKSKSGHPGVVMGAAPMAYSLFTKELR
                     INPAQPNWVNRDRFILSAGHGSMLLYGLLHLSGFEEVSMDEIKNFRQWGSKTPGHPEF
                     GHTAGVDATTGPLGQGISTATGFAQAERFLAAKYNRDGFPIFDHYTYVICGDGDLMEG
                     VSAEAASYAGLQKLEKLIVLYDSNDINLDGETKDSFTEDVRARYNAYGWHTDLVTDGT
                     DVDAIFAAIEKAKAAGKPSLIEIKTVIGHGSPNKQGTNAVHGAPLGAEETEATRKALD
                     WNYAPFEIPAEVYADFKANVADRGAAAYDAWVKLVEDYKVAHPELAAEVTAILEGRDV
                     VEIKPEDFPVYENGFSQATRNSSQDALNAAAKVLPTFLGGSADLAHSNMTYIKEDGLQ
                     DSVNPLNRNIQFGVREFAMGTILNGMAAHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPIEHLAGLRAMPNLNVFRPADARETQAAWYLSLTSKS
                     TPSALVLTRQNLTVEEGTDFDKVAKGAYVVYEVAGFDTILLASGSEVNLAVKAAKELE
                     AAGTKVRVVSVPSTELFDAQDAAYKEEILPNAIRRRLAIEMGATQSWYKYVGLDGKVL
                     GIDTFGASAPAQTVIDNYGFTVENVVKLVGEL"
     gene            complement(3808..4899)
                     /gene="ulaG"
                     /locus_tag="AC1PNG_000325"
     CDS             complement(3808..4899)
                     /gene="ulaG"
                     /locus_tag="AC1PNG_000325"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HIP6700862.1"
                     /translation="MAKVQDITRESWILSTFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGVWIKTPGGANVVMDLWSSRGKSTKKVKDMVWGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHVHSDHMDISTAAAIINNPKLDHVKFVGPVESGDIWEKWGVPAD
                     RIIRIAPGDSFEFKDIKVHAVESFDRTCLVTLPIEGYDENGGKLAGLPVTDELMARKA
                     VNYVFETPGGTIYHGADSHFSNYFAKHGRDFNIDVAINNYGDNPIGIQDKMTSIDLLR
                     MAENLRAKVIIPVHYDIWSNFMASTDEILQLWKMRKERLQYDFHPFIWEVGGKYTYPM
                     DKDRIEYHHPRGFDDCFLEDSNIQFKALL"
     gene            complement(5009..6697)
                     /locus_tag="AC1PNG_000326"
     CDS             complement(5009..6697)
                     /locus_tag="AC1PNG_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIP6700863.1"
                     /translation="MLLDKSSCALLRYLIGLQEPETIMAISKGLQQSRRKIYYHLEKI
                     NDALPTTVESIESLPRLGIRLTEAQKLACRDLLDSIDSYTYVMSMPERIQLMLVYICI
                     TPERVTIERLMELTEVSRNTVLNDLNEIRTQLAQEQYKMTLYVSKSHGYSLVCHPLNK
                     IQYVHSLLYTIFLDANKGFLQVLEDKLFELVGCQLLFSEEMAQFLQGQVSQLEKKLGK
                     KINRYEIEFMLKVLPFLLLSYRNMELDDLEERAILKEFEQVRERIEYRVAQELKDALL
                     QTFDLKLDEIEISLIAILLLSYRKDSDVHVFSQDFIDLKRVVERFIHHFEIHSPFELE
                     NPDELAKHLLAHCKALLFRKTYGILSKNPMVQQIQDKYSTLFAMTRVSGKILEEEWQI
                     ELSDGDIAYLTIHLGGAIRRSGTRKAQTQIVYLICDEGVSVQRLLYKQCQHHLPDKKI
                     GAVFTTEQFKSVEDLLEVDFLITTSDGLETDFPLVQVHPILDFDDVLNMTHFAKYRSL
                     ADEKQVFAVELDKLLSGYIADGRTARELKQRLQKLITNELLTNLSSQDIETDLY"
     gene            complement(6822..7439)
                     /locus_tag="AC1PNG_000327"
     CDS             complement(6822..7439)
                     /locus_tag="AC1PNG_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700864.1"
                     /translation="MRKFLQDSLTETAYYLELILSAILGLALLALSTLLFADLLSSLA
                     GDIQADTFIQNFLSRAMTLAVGVEFIKMLYKQSPSTVIEVLLVAIARQLIVEHGSSSD
                     YLIGIISVAILFAVRKYLFTQFDDSNNIIMRASQKVKVANIIARVKIPSEGSETLRDF
                     VTRKLNEEEKTIAIGACVYLKNVALRIDNMHGELITRVEIIKSIY"
     gene            complement(7524..8240)
                     /locus_tag="AC1PNG_000328"
     CDS             complement(7524..8240)
                     /locus_tag="AC1PNG_000328"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HIP6700865.1"
                     /translation="MPKDLQEMRQRVYEANIALPAHGLVKFTWGNVSEVCRELGRIVI
                     KPSGVDYEKLSPENMVITDLDGNVVEGDLNPSSDLATHVALYKAWPNVHAVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLTAEEVNSAYEKETGAVIIEEFERRGI
                     DPVAVPGIVVRNHGPFTWGKDPAQAVYHSVVLEEVARMNRYTEQINPRVEPAPSYIMD
                     KHYLRKHGPNAYYGQKGDEH"
     gene            complement(8240..9103)
                     /locus_tag="AC1PNG_000329"
     CDS             complement(8240..9103)
                     /locus_tag="AC1PNG_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HIP6700866.1"
                     /translation="MARPIGIYEKATPKHFTWKERLEFAKELGFDFVEMSVDESDARL
                     ARLEWTKEERLDLVKAIYETGVRIPTICFSGHRRYPLGSNAPALEAKSLETMKQCIEL
                     AQDLGVRVIQLAGYDVYYEEKSPETRERFIKNLRQACDWAEQAQVMLAIEIMDDPFIN
                     SIEKYLAVEKEIDSPYLFVYPDTGNVSAWHNDIYSEFYLGHRSIAALHLKDTYAVTEN
                     SKGQFRDVPFGQGCVDWDNMFAVLKKTNYQGPFLIEMWSENCETVEETRAAIKEAQDF
                     LYPLIEKAGLN"
     gene            complement(9109..9771)
                     /locus_tag="AC1PNG_000330"
     CDS             complement(9109..9771)
                     /locus_tag="AC1PNG_000330"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014622185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HIP6700867.1"
                     /translation="MKHIPNLQVALDHSDLQGAIKAAVSVGHEVDVIEAGTVCLLQVG
                     SELVEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICSATIPTMAAALKA
                     IKEVRGDKGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNKV
                     KKLVDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAAAAREFKDEIRRI
                     WG"
     gene            complement(9782..10267)
                     /locus_tag="AC1PNG_000331"
     CDS             complement(9782..10267)
                     /locus_tag="AC1PNG_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIP6700868.1"
                     /translation="MNLQKSFTENNSIRLGLTAETWQEAVHKAVEPLIDSGAATEEYY
                     DAIIASTEEYGPYYVLMPGMAMPHAQAGVGVLKDSFALITLTKPVVFSDGKEVSVLLT
                     LAATDPKIHTSVAIPQIIALFELENSIERLIACQTPEEVLAMVEESKTSPYLEGLDLE
                     S"
     gene            complement(10330..10608)
                     /locus_tag="AC1PNG_000332"
     CDS             complement(10330..10608)
                     /locus_tag="AC1PNG_000332"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIP6700869.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDFQSASCSVGEA
                     KGLAAGYDIVVASNHLISELEGRTKGHLVGLDNLMDDNEIKTKLQAVL"
     gene            complement(10667..12115)
                     /locus_tag="AC1PNG_000333"
     CDS             complement(10667..12115)
                     /locus_tag="AC1PNG_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIP6700870.1"
                     /translation="MDFLQTPLNWFSQNILQNPAFFVGLLVLVGYALLKKKPHDVFAG
                     FIKATVGYMILNVAAGGLVTTFRPILAALNYKFQIDAAVIDPYFGLAAANGKIAEEFP
                     NFVSAATTALLIGFGVNILLVALRKVTKVRTLFITGHIMVQQAATVSLMVLFLIPALR
                     NQFGTAAIGIICGIYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKIAPKLGK
                     KEENLDNLKLPSFLNIFHDTVVASATLMLVFFGVILFILGPEIMANPEVITSGAPYNP
                     AKQAFFMYVIQTAFTFSVYLFILMQGVRMFVAELTNAFQGISSKLLPGSFPAVDVAAS
                     YGFGSSNAVLSGFAFGLIGQLITIVLLIVFKSPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAAILSFISGVLQVALGAVCVGLLELSGGYHGNIDFEVPWLPFGFAFKYLGIAGY
                     VLVCLFLLAIPQLQFAKAKDKEAYYRGEAQAE"
     gene            complement(12463..18768)
                     /locus_tag="AC1PNG_000334"
     CDS             complement(12463..18768)
                     /locus_tag="AC1PNG_000334"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HIP6700871.1"
                     /translation="MKKKSVQNMKRTGMLEKKQIFGIRKLNVGVASVGIAAALFLSGA
                     GQLVQAEEVVLPASTSSTTLSEQDGGAVEATTTAIEATDSMVAEATAVTETADATAPE
                     EAPIEEGSIRLHFENVDETAPESQGLWTWGGVAEPSDSNQWPTDTANFSSSQVDDYGH
                     YVDIKKSETPGTIGYLVLKNGEKITESDQKVELLVPEQNEAWISSDYSVSSYEPLKDE
                     NVLRINYTREDNNYEGWGVWTWGDTTEASDGWPTGAVDFKLGKYGAYVDIPLSNGLDS
                     KLGFLLINQNNPDLAGNKTIDLAFADRKRHSQIFLRNGDDKVYTNPYFIEEKVELDTS
                     KATPGTKNVTVEASVKAPFNYNESGLVSVTVTNPENAEIVKMEVDTSAIGGGLVPIST
                     ELNRVTIKATSSTAPGTYSLPVKVYDKDNGYYETKLDVTITERVKADGEKDWDEQVIY
                     FMMTDRFYNGDTSNDKLVEGTVTNPRGLYQGGDFKGVTAKLDYLKELGVDSIWLTPIV
                     ENIPQNVGSVKDGEYYAYHGYWASNFEKLNPHLGSLADFHELIDAAAEKGINIVVDVV
                     LNHAGYGTEETFAGMVRTKEEDKQGDDQLGSLSGLPDFKTEEAAVRNQLVAWQASWLE
                     RSTTAKGNSIYAFRVDTVKHVDDTTWQHFKNELVDRDPDFHLIGETWGANYKDTKGDL
                     GIGTMDSLLDFGFKDIAKYLVNGQLKAAGKELEERSKVLTSAASLGQFLGSHDEDGFL
                     YSLGGAEKEGNLDKLKLAASLLITAKGQPVIYYGEEVGQSGQNNWPVYDNRYDFDWSK
                     VETSDITDHYQKLLAFRNANSTLLSRGDTSTLAGNDSQGWLISKRSYQDQAAYLVFST
                     NTESKEMALEVSSKDVVVTDAYTGKSYQAIEKGGKWVVQVELPTIGQGGTMLLQTEAG
                     DIVNASVQGATEEPIEAGYFRVHFKTLPSDNLSSLGLWTWDDVEKPSSDVGAWPTGAT
                     NFSTAKQDDYGYYLDIKMKDETASKISLLINNTSGDNITGDKTIERISTKMNEAWFDE
                     NYQLSLYQPLKEGCIRINYFRTDGNYDQKGLWIWGDVTDLTLGDWPNGIDFENQGKYG
                     AYIDVKLTDLPSSIGFLLLDESKSGDDVKIQQKDYSFKDLKNQTQIFLKDDDATIYTN
                     PYFVNNVRVTGVSHVSLTALEAAFTTLEGADKDSILEKLSVTDKNGQTVAVTDLVLDL
                     TSNKVRVLGDFNQENAGYTLKYGNDSFTTTMSWQLKDELYAYDGELGARVRQAGSVVD
                     MTLWSPSADSVAVVLYDKDDQSKVVGKLAMTKGDKGQWDLELNSQSGLGIADYRGYYY
                     HYEITRGGQSVLVLDPYAKSLAEWNSDLADTDPSYRIAKAAIVDPAEVGPSDLNYATI
                     PNFNQREDAIIYEAHVRDFTSDPAISDELTAQFGTFAAFAERLSYLKDLGVTHIQLLP
                     VMSYYFVNELKNAERMSEYASSNSNYNWGYDPQSYFAFTGMYSTDPTDPMKRIEEFKN
                     LVNEIHKQGMGVILDVVYNHTSKTFLFEDLEPNYYHFMEADGTAKSSFGGGRLGTTHY
                     MSRRVLVDSIKYLVDEFKVDGFRFDMMGDHDAEAIELAFTEAQKLNPNIIMLGEGWRT
                     FTGDANQPVQPADQDWMSSTDTVAVFSDEIRNTLKSGYPNEGQPAFITGGAKSVESVF
                     NNIKAQPGNFLADDPGDVIQYIAAHDNLTLFDIIAQSIKKDPSIAENYTEIHQRQRLG
                     NLLVLTAQGTPFIHSGQEYGRTKQFRHPDYKEPVSEDKVPNKAHLLTNADGTPFDYPY
                     YIHDSYDSSDAVNKFDWTKATDEALYPENTRTQAFTKGLIALRKSTDAFRLGTKEEVE
                     QKVSLISIPGQNGIAKNDVVIAYQTIASNGDRYAVFVNADSKERSFVLSDIYKELLKG
                     HVLVDGKRAGIAALTDLVGVTLTDDAVVLAPLTATVIRLPYITTAVPDTAPTAEEKPS
                     LDFTTKERTEEKILPIVEEIRYDASLSTDQSYVLQEGKTGKKVLVYQDVLIDGKVVAT
                     NLLSETVVDSETRIVVKGSMEAKKEAEKPSLSTTAQASGQATSGASKASLPITGDRQS
                     DLALLGLGLAGLGLTVAAQGRTKKSEE"
     gene            complement(18949..19890)
                     /locus_tag="AC1PNG_000335"
     CDS             complement(18949..19890)
                     /locus_tag="AC1PNG_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIP6700872.1"
                     /translation="MTTLADVARLANVSKMTVSRVINHPEQVTQELRTLVTAAMEELN
                     YKPNVAAKALAQQRTLVVQAVILEKMDVVEPYYIDLLAGIADELNHRNYTLQLVTNPS
                     QITDQCDGYIVTGARKNDYPWLKGLRKPLILFGENQEGLPFVDSDNRLATRSATQFAL
                     SKGYERIVFVGIDLPEAFEKGREEGYKDAMQGRETHLYRLENRSRVAEEFVKNLDYLP
                     ENTCFICASDRLALGVTRGLQALGKSIPNDVGVIGFDGFFLDRMSRPALTTMKQPIRQ
                     MGALSVKHLMTILDGKPLARQGHYCQATLIERETTPQ"
     gene            complement(20255..21001)
                     /locus_tag="AC1PNG_000336"
     CDS             complement(20255..21001)
                     /locus_tag="AC1PNG_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIP6700873.1"
                     /translation="MIQASGVKLTFGGQQVLKGIDVEVNPGEVVVILGPSGSGKTTLL
                     RSLNFLEKADAGHLVFGEDSYDLAQMTKKEIAGIRKRTAFVFQNYNLFANKTALQNVT
                     EGLIVARKVPKQEAERIGKAALDKVGLSGKYGFYPSQLSGGQQQRVGIARAIATNPEV
                     IYFDEPTSALDPELIGEVLAVMMQLAKEGMTMVVVTHEMGFARAVADRVIFMDGGRII
                     EEGRPEDIFDRPQEERTKQFLARIQKVDVE"
     gene            complement(21011..21670)
                     /locus_tag="AC1PNG_000337"
     CDS             complement(21011..21670)
                     /locus_tag="AC1PNG_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIP6700874.1"
                     /translation="MTERVWQLLLDSFSQILVPGLLVTIPLTILSFTFGLLIAIGTAL
                     VQIAQIPILKQVARFYIWVVRGTPLLVQLYVIFFGLPSLGIVLDAFPSAVLVFSVNTG
                     AYAAETIRASIESVPKGQLEAGYSVGMSFAQTMRRIILPQAFRVAFPPLSNTLIGLVK
                     DTSLAANITVLEMFMATQQIAARTYEPFALYCEVALVYLLFSTILTKLQAYGEKKLAV
                     Y"
     gene            complement(21683..>22490)
                     /locus_tag="AC1PNG_000338"
     CDS             complement(21683..>22490)
                     /locus_tag="AC1PNG_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIP6700875.1"
                     /translation="IMKKIAMFTSLLFAGIVLAACNSSAGISNSTDQTLLAQIKEEGV
                     IQIGTEGAYAPYSYHDESGKLVGYDVEVAEAVAEKLGVKVEFVETEWDSMIAGLDAAR
                     FDTIANQVGITDERKAKYDFSTPYTYIYGALVTQKDNTDITSFADLSGKKSANSLTSN
                     WADLARENGAEVVGVDGFSQAVELLNTGRVDATINDNLVYLDYVKQHADAPIKVVTLT
                     DDVSTTAFPVAKGNEDLAKEIDAALAELASEGKLVEISNKYFGEDVSKAK"
BASE COUNT         6151 a         5282 c         4507 g         6550 t
ORIGIN      
        1 tcatttattt tcatgaaata tgttgtataa atcttaattt ttgtgaaaat ctaacgaaat
       61 tttcgcgttt gtaataatcc tccaaagtct ctcccgactg ggttactgta atccatttcg
      121 ggagaatctt agatagtagg taaagcggaa tgagggcagg ataccaaatg aacatgactt
      181 caaagatgaa acggtcgttg ttgcctcttg aaatgtaacg ttggcggtat tcttctttga
      241 gagaagggtg gggaatatag atatgaaatg gcacaccacc gcgtatagac atggccagca
      301 acattctcat cagaaaattc agcgctacta ggactgtctt tccttgtcta tgaagagtgt
      361 aggccttatt gacatagatt gttttatagc ctttatcctt cagtgtgcgt ttggtggatt
      421 ttcgctcctc tttcgttaaa tgacgaacac tgctattatc gtacttaatg tcggacattt
      481 ctaagtccac aacatctgta tatttcttag caagtgtcgt ttttcccata cctggaaagg
      541 caaataaaaa cataggcttt cctccttatt cctcagtaga atagcagagc atccaaaaag
      601 ttttcttaag ttgcccctat tttgcccctc atttctaaaa ataaaccaaa gaaaaaccct
      661 ctgaaatcct gatttcaaag ggtttttaga ttatatgcaa aaagagcaca cattcgacct
      721 cgcttagggc tgctggattc ctcccctgac ccgcttcacg cacgaatgtt gctccgttta
      781 atattataac acaatttggc cagagtgcaa gtttggaata ttaactagct atccgaagtt
      841 gcttcctctt gcgtcttcat gataagttta gcagccttct gtctttctct gcgttgtctg
      901 aaaaaatctt gcatgatatt ggcacattcc gcttctaaaa tacctgtttc taccttgaca
      961 cgatggttga gccgctcatc tgtcagaatg tcatagaggc tccccgcagc tccaaacttt
     1021 ttgttggttg ctccatagat gacctgcgga atgcgtgcta ggccaatagc tccgctacac
     1081 atcacacaag gttcaatcgt cacaaaaagg gtcgaatcaa gcaagcgcca attcccctca
     1141 acattgttag cctcttggat tgccataacc tcagcgtgca tgattgcctg attgagttcc
     1201 tcacgcgcat tatgccctct gccaataatc tggccatcct tgacaatcac acaaccaatt
     1261 ggaatttcat ccttttctag cgactttcta gcttcctgca aggcttgagt cataaaatac
     1321 tctttttctt catgtgtata gttcataaat ctattatagc acagccctga taacagttca
     1381 aaatcacttc tttattctct gctatcaaaa tcaatctaac actacttctt ccttatcaca
     1441 catttctttc aaaattctgc ccttctatta acttaaaaat ataaaaatac ctcctcagtc
     1501 gagaaggtag ggggagcaat attgatattt acagatatta atatatcatc tattgtcgat
     1561 attgtcaaga aaatttttaa aaaaattcaa aaacagcctc gaaaggctgt ctctgacatt
     1621 ttctattaca attcgccaac taatttcaca acattttcta ctgtgaagcc gtagttgtca
     1681 ataactgttt gggctggagc tgaggcaccg aatgtgtcga taccaaggac tttaccgtca
     1741 agaccgacat acttgtacca actttgagtt gcacccattt caattgccaa gcgacgacgg
     1801 atagcatttg gaaggatttc ttccttgtaa gctgcgtctt gtgcatcgaa gagttcagtt
     1861 gatggcacag aaaccacacg aaccttagta cctgctgctt ctagttcttt ggcagcttta
     1921 acagccaagt tcacttctga accagatgca agaaggattg tatcaaaccc tgctacttca
     1981 tagacaacat aagccccttt tgcaaccttg tcaaagtctg ttccttcttc aactgtcaag
     2041 ttttgacgag tgagaacaag ggctgatggt gtagacttgc tagtcaatga caagtaccaa
     2101 gctgcctgtg tttcacgcgc atctgctggg cggaagacat tgaggtttgg catagcacga
     2161 agtcctgcca agtgctcaat cggctcgtga gttggaccat cttcaccaac tgcgatagag
     2221 tcatgagtga agacataagt cacaggaagt ccttgaagag ctgacaaacg aactgctgct
     2281 tttacatagt ctgagaagac aaagaaggta ccaccataga cacgaagtcc gccgtgggct
     2341 gccataccat tcaagatagt tcccatggca aattcacgca caccgaactg aatattgcgg
     2401 ttgagtggat ttactgaatc ttgcaaacca tcttccttga tgtaggtcat gtttgagtga
     2461 gccaagtcag ctgaaccacc taagaaagtt ggcaatactt tagccgctgc gttgagggca
     2521 tcttgtgatg agttacgagt tgcttgtgag aagccatttt cgtaaaccgg gaagtcttct
     2581 ggcttaattt caactacgtc acgaccttcc aagatagctg ttacttcagc tgctaattct
     2641 ggatgtgcta ctttgtagtc ttcaactaat ttcacccaag catcgtaggc tgctgcaccg
     2701 cgatcagcta cattagcttt gaagtccgca tagacttcag ctggaatttc aaatggtgca
     2761 tagttccaat caagcgcctt acgagttgct tctgtttctt ctgcaccaag tggagcaccg
     2821 tgaacggcat ttgtaccttg tttgtttggc gaaccatgac cgataacagt cttgatttcg
     2881 ataagagatg gcttacctgc tgccttagct ttttcaatag cagcaaagat ggcatctaca
     2941 tctgtaccgt ctgttacgag gtctgtatgc cagccatagg cattgtaacg ggcacgtaca
     3001 tcttctgtga aggaatcttt tgtttcgcca tccaagttga tgtcatttga atcgtaaaga
     3061 acgatgagtt tttctaattt ttgaagacct gcgtaagaag ccgcttctgc tgaaacaccc
     3121 tccatcaagt cgccatcacc acagattacg taagtatagt ggtcaaagat tgggaaacca
     3181 tcgcggttgt acttagctgc aaggaaacgc tctgcttgtg caaaacctgt cgcagttgag
     3241 ataccttgtc caagaggacc tgttgttgca tctacacctg ctgtgtgacc aaattctggg
     3301 tgacctggcg tttttgatcc ccactgacgg aagttcttga tctcatccat actcacttct
     3361 tcaaaacctg aaaggtgaag aagtccgtaa agcaacattg acccatgacc tgctgataag
     3421 ataaagcggt cacggttcac ccagtttggc tgagctgggt tgatacgaag ttctttagta
     3481 aaaaggctgt aggccattgg agccgcaccc atgacgacac ctgggtgacc tgattttgat
     3541 ttttcgatgg catcaatacc aaggaaacga atcgcattga ctgaaagatt tgacatgttt
     3601 ttctcctttt ttaaagtcat gaatttatta taccataaag cgttttctat gtctgtttga
     3661 aatgaagcga gaaataaatt gtccgtagaa aagaggcagg actttcgtcc cgcccttgga
     3721 gtgaacagta gtaattcgca catttcatac tttatcagag tttatagtgc ctctcctcgt
     3781 ctctatgggg caagagagtt ctaaatctta taacaaagcc ttaaattgaa tatttgagtc
     3841 ttctaagaag caatcatcaa agccacgtgg gtggtgatat tcgatacggt ccttgtccat
     3901 cggataagta tacttgccac caacttccca gatgaatgga tggaaatcgt attgcaagcg
     3961 ttccttacgc attttccaga gctgcaagat ttcatctgtt gacgccatga agttagacca
     4021 gatatcgtag tgaacaggga tgattacctt agcacgcaag ttttctgcca tacgaagaag
     4081 gtcgattgat gtcatcttgt cctggatacc gattgggttg tcaccgtagt tgttgatagc
     4141 aacgtcgatg ttgaagtcac gaccgtgttt tgcaaagtag tttgagaagt gggagtctgc
     4201 accgtggtag atggttccgc caggcgtttc aaagacatag ttgactgcct tgcgagccat
     4261 gagttcatct gtcacaggaa gtcctgctaa tttaccacca ttttcgtcat aaccttcgat
     4321 tggtaaggtc accaagcaag tacggtcgaa ggattctaca gcgtggacct tgatgtcttt
     4381 gaactcaaag ctatcacctg gtgcgatacg gatgatacgg tcagctggaa caccccattt
     4441 ttcccaaatg tcaccacttt caaccggtcc gacaaatttg acgtggtcca actttggatt
     4501 gttgatgatg gcagcggctg tgctgatgtc catgtggtca ctgtggacgt gcgatactag
     4561 atagtagtcc aattcattga tggcaaatgg atcgataacc attggctgca cacgcaagtt
     4621 tggttgcaat ttgcgcacac cggccatgtt agccatctgg tgtccccaaa ccatatcttt
     4681 cactttttta gtggattttc cacgtgatga ccagaggtcc atgaccacgt tagctccacc
     4741 tggtgtttta atccagacac cacagtttcc aagccaccac atagcaaagt tgccttctgg
     4801 cactacttct tcttcaattt cttcattgag ccaagttccc cattctggga aggttgaaag
     4861 aatccaggat tctcttgtaa tatcttgaac tttagccatt ttccattcct cctataaggt
     4921 acgtttctat gcttctagta tatgctccaa aggcaacaaa aaaaagacca aataaaacac
     4981 aggcttgtgt tcaaacttgg actttaccct aatacagatc tgtttcaata tcttgactgg
     5041 ataaattggt taataattca ttggtaatca gtttttgtag ccgttgtttg agttctcgag
     5101 cggtccttcc gtctgcgata tatcccgaca aaagcttgtc tagttctact gcaaaaactt
     5161 gtttttcatc tgccaaacta cgatattttg caaaatgagt catgtttagc acatcatcaa
     5221 aatctaaaat ggggtgaacc tgcaccaaag ggaaatccgt ttccaaccca tcgctggttg
     5281 taatcaagaa atcaacctct agtaagtcct cgacactctt gaattgttca gtggtaaaga
     5341 cggcgccaat cttcttatct ggcaaatgat gctggcattg tttataaagt aagcgttgga
     5401 ctgacacccc ttcatcacag attaaataga caatctgtgt ctgtgccttg cgcgtcccac
     5461 tgcgtcgaat agctcctcct agatgaatcg tcaaataggc aatatctcca tcggataact
     5521 caatctgcca ctcctcctcc aaaatctttc cagaaacccg tgtcatagca aacagggtgc
     5581 tgtatttatc ttggatttgt tggaccattg gatttttaga taggatgccg taggtttttc
     5641 tgaacaacag agccttacaa tgtgccaaga gatgtttggc taattcgtca ggattttcca
     5701 attcaaatgg tgaatgtatc tcaaaatgat gaataaatcg ctctaccact cgcttcaagt
     5761 caatgaaatc ttggctgaat acatgaacat cgctgtcctt tcgataggat agaagtaaaa
     5821 tggcaatcag ggagatttct atttcatcca atttcaaatc aaaggtttgc aacaaggcat
     5881 ctttcaattc ctgagcaact cggtactcga tacgctctcg aacttgttca aattctttga
     5941 gaatggctcg ttcttccaag tcatccagct ccatattgcg atagctaagg agcaagaaag
     6001 gcaatacttt gagcataaac tcgatttcgt agcgattgat tttcttaccc agtttctttt
     6061 ctagctgcga tacttgtccc tgtaaaaatt gtgccatttc ttctgagaag agcaactgac
     6121 accccaccaa ctcaaataac ttatcttcta gaacctgcaa aaaaccttta ttagcatcca
     6181 agaagattgt atagagaagt gagtggacat attggatttt atttaaaggg tgacaaacga
     6241 gactatagcc atgactctta gacacataga gggtcatctt gtattgttct tgagccaact
     6301 gggttcgaat ttcattcaaa tcatttaaga ccgtattacg agagacttct gtcaactcca
     6361 ttagacgttc aatggtcact ctttctggtg tgatacagat atataccaac atcagctgaa
     6421 tgcgctcagg catgctcatg acgtaggtgt acgaatcaat ggaatcgaga agatctcgac
     6481 aggctagttt ttgcgcttcg gtaagtcgaa taccgaggcg aggtaagctt tcaatactct
     6541 ctacagttgt tggtagggca tcgtttattt tttctagatg gtaatagatc tttcgacgtg
     6601 attgttgcaa gcccttggaa atagccatga ttgtctcagg ttcctgcaga ccaatcaggt
     6661 acctcagtag agcgcaacta ctcttatcta ataacaagat tctcctcctc tttcccccgt
     6721 cccgttacta tcttaacata attatctggg atgttctaat aattattaaa attcatctga
     6781 tcattacttg ctttatgttg cataaatatt tgtagttaga attaatagat gcttttaata
     6841 atttctaccc gggtaatcag ttctccgtgc atgttatcta tccgaagggc aacatttttc
     6901 aaatatacac aggcaccaat agcaatggtt ttctcttctt cattgagttt tcgtgtcaca
     6961 aaatctctca aagtctcgct accttcactt ggaattttta ctcgtgcgat gatattagcc
     7021 acttttactt tctggctggc gcgcatgata atattattgg aatcatcaaa ctgagtgaag
     7081 aggtattttc taactgcaaa taggatggcg accgaaataa ttccaatcag gtaatcacta
     7141 ctagaaccat gctctacaat cagttggcga gcaatagcaa ccaggagtac ctcaatcacg
     7201 gtgctaggtg actgtttgta aagcattttg ataaattcga ctccgactgc caaggtcatt
     7261 gcacgactca gaaaattttg aataaacgta tccgcttgaa tatcaccagc taatgagctg
     7321 agtaggtctg caaaaagaag cgttgacaag gctaaaagtg ctaagccaag tatggcagat
     7381 aaaatcaatt ctaaatagta ggctgtctct gtcagactat cctgtaaaaa tttcctcatc
     7441 tttctcctca tcaaaaggga aaaagcagtt ctcctttttg gggagtctgc tacttttccc
     7501 tgctgttttt ttcacacaag aacttaatgt tcatcaccct tttgtccata gtaggcattg
     7561 ggaccatgtt tacggaggta gtgcttgtcc atgatatagc ttggcgctgg ctccacccgt
     7621 ggattgattt gttcggtata gcggttcatc cgagccactt cctcaaggac aacgctatgg
     7681 taaactgcct gagcgggatc cttgccccag gtaaatggtc cgtggttacg gacaacaata
     7741 cccggaacgg ccactggatc aatacctcgg cgttcaaatt cctcgatgat gactgctcct
     7801 gtttcttttt cataggccga gttcacttcc tcagccgtca aagaacgagc acatggtacc
     7861 ggaccataga aatagtccgc atgggtcgtc ccataaaatg gaatgtcacg acctgcctga
     7921 gcccaaccca ctgcttctgt cgaatgtgta tgcacaacgg catggacatt tggccaagcc
     7981 ttgtagaggg caacgtgggt cgccaaatca gacgaaggat tgaggtcacc ttctaccaca
     8041 tttccgtcca agtccgtaat gaccatattt tctggtgaca atttctcata gtccacacca
     8101 gacggcttga tgacgatgcg accaagttca cggcagactt ctgatacgtt gccccaggta
     8161 aacttgacga gcccatgagc aggcagggcg atattggctt catagacccg ttgacgcatt
     8221 tcctgtaaat cttttggcat tagttcaacc ccgctttctc aatcaatgga tagaggaaat
     8281 cttgtgcctc cttgatagct gctcgtgttt cctcaaccgt ttcacaattc tctgaccaca
     8341 tttcaatcaa gaatggacct tggtagttgg tctttttcaa tactgcaaac atattgtccc
     8401 agtccacaca gccctgaccg aatgggacat cacggaattg acctttggaa ttttcagtta
     8461 ccgcataggt atccttgagg tgaagggctg cgatagaacg gtgtcccaga taaaattcgc
     8521 tataaatgtc attatgccaa gcagacacgt ttcccgtatc tggatagaca aagagataag
     8581 gcgagtcgat ttctttttca acagctaggt atttttcaat ggagttgata aatggatcat
     8641 ccataatttc aatggccaac atgacctgag cctgttctgc ccagtcgcaa gcctgacgga
     8701 gattcttgat gaaacgctct ctggtttcag gtgatttttc ctcgtagtaa acatcatagc
     8761 cagccaactg gatgactcga acgcccaaat cctgagccag ttcgatacac tgtttcatgg
     8821 tttctaagga cttggcttca agtgcagggg cattggagcc aagcgggtag cgacggtgac
     8881 ctgagaaaca aatggtcgga atgcggacac ctgtttcata gatggccttg accaagtcta
     8941 aacgctcttc cttggtccac tccaaacgag ccaaacgagc gtcgctctca tccaccgaca
     9001 tttccacaaa gtcgaaacct aattctttgg caaactccaa gcgttccttc catgtgaaat
     9061 gcttgggcgt tgccttttca tatattccga ttggtcgtgc cataggcctt acccccagat
     9121 acgacggatt tcgtctttaa attcacgcgc cgcagctgct ggatctgctg cttctgtgat
     9181 accgcgacct gcgataaagg tgaagacatc tacaccctca aagagtttga gggtatccac
     9241 atcaagacca cctgttacag aaacacggaa gcccatgtca accaattttt tcactttatt
     9301 gaggtctttt tcaccccagg tttcgccagc aaggagggcg tcacgcgatt ggtggtaaat
     9361 cgcttgggaa atacctgcgt ccaaccaaag ctgagcttgt tcaaacgtcc aatcaccgta
     9421 aagttcgatt tggatttcac ccttgtcgcc acgcacttcc ttgatagcct tcaaagcagc
     9481 tgccatagtt ggaatagttg ctgaacaaat acaagtcatc cagtccgcac cacgaacagc
     9541 attgttttta gccacagtac cacccgcatc cgcacacttg gtatctgcaa cgataatttt
     9601 gtctgggaag aggctacgaa gaacttctac caattcgcta ccaacttgaa gcaaacaaac
     9661 ggtacctgcc tcgataacat ccacttcatg accaacggag acagctgctt taatcgctcc
     9721 ctgcaagtct gaatggtcaa gggcgacttg taaatttgga atatgtttca tgttttttct
     9781 cctaactttc taaatctaaa ccttctaggt aaggactggt tttggactct tccaccattg
     9841 ccaagacttc ctcaggagtt tgacaagcaa tcaaacgctc aatcgaattt tccaattcaa
     9901 agagggcaat gatttgtgga atagctacgg atgtatgaat ctttggatcg gtagctgcaa
     9961 gagtgagcag aacagacact tctttcccat ctgagaagac gactggtttt gtcaaggtaa
    10021 tcaaagcaaa ggaatccttc aagacgccga cacctgcttg ggcgtgaggc atagccatac
    10081 caggcatgag cacatagtaa ggtccatatt cttcagttga tgcgatgatc gcgtcgtagt
    10141 attcttctgt cgcagcaccg ctgtcaatca agggctcaac agccttgtga acagcctcct
    10201 gccaggtttc cgctgtcaat cccaagcgaa tggaattatt ttctgtaaat gatttttgta
    10261 aattcatagt ttttcccttc tacacgctat atgaaagggg gagggaaact ccctacccct
    10321 aacggctatt tatagcactg cttgtaattt tgttttgatt tcgttgtcgt ccatgaggtt
    10381 gtcaagacca accaaatgac ctttcgtgcg accttccaac tcagaaatca agtggttaga
    10441 ggctacgaca atatcatatc ctgccgctaa accttttgct tcaccgactg agcaagaagc
    10501 tgattgaaaa tctgttacgc cgagttgacg aagggcgttt tctaccttca tcttgatgac
    10561 catagatgag cccatgccat ttccgcaggc tgttaaaact ttaaccataa tttcactcct
    10621 attttatttt taatacatta tgctctgatt ttacagacca aggatactat tctgcttgtg
    10681 cctcaccacg atagtatgct tccttatctt ttgctttcgc aaattgaagt tgcggaatag
    10741 ccaataagaa gaggcagaca agaacataac cagcaatacc taggtactta aatgcaaatc
    10801 caaatggtaa ccatggcact tcaaagtcaa tgtttccgtg gtaaccaccg ctcaattcaa
    10861 gcaagccaac gcagactgca ccaagagcaa cttgaaggac acctgagatg aaggaaagga
    10921 tggctgccgc tttccaacca ccacgtttgt cagcgtacac tgcgatagct gcgttatcga
    10981 agaatacagg tacgaaacca gtgatgatga ggattggact cttgaagaca atcaagagga
    11041 cgattgtaat caactgaccg attaaaccga aggcaaaacc tgaaagaact gcgttagatg
    11101 aaccgaagcc gtaagaagct gcaacgtcca ccgctgggaa tgaaccagga agcaatttgc
    11161 ttgagatacc ttggaaggca tttgtcaatt ctgctacgaa catgcggaca ccttgcatca
    11221 aaatgaaaag atatactgag aaggtgaagg ctgtttgaat cacatacata aagaaggctt
    11281 gtttagctgg gttgtatgga gcacctgaag tgataacttc tggatttgcc atgatttctg
    11341 gacccaagat gaacaaaatc acaccgaaga acacaagcat caatgtcgct gaagcaacga
    11401 ctgtatcgtg gaagatgttg aggaagctag gtagcttcaa gttatccaag ttttcttctt
    11461 ttttaccaag ttttggtgca atcttatcga caaaccagat tgcgaattgt tgttggtgac
    11521 cgatggcaaa tccaccgcca cctgtcaatc tttgcgttgc ttcaactgtc atatttgagc
    11581 tgactgccca gtagatacca cagatgatac cgatcgctgc tgtaccgaat tggttacgaa
    11641 gagccgggat gaggaagagc accatcaatg agactgttgc cgcttgttgt accatgatgt
    11701 gtccagtgat gaacagagta cgtactttgg taaccttacg tagtgccacc aagaggatgt
    11761 tgacaccgaa accaatcaag agtgctgtag tagctgctga tacgaaatta gggaactcct
    11821 cagcaatttt cccatttgct gctgcaagac caaagtaagg gtcaataact gccgcatcaa
    11881 tttggaactt atagttcaag gccgccaaaa tcggacggaa ggttgttacc aaaccaccag
    11941 ccgctacgtt caaaatcata tagccgactg tcgctttgat aaaccctgca aaaacatcat
    12001 gaggcttttt ctttaagagg gcatagccca ccaatactaa aagacctacg aagaaggctg
    12061 gattctgcag gatattctgt gagaaccagt ttaatggggt ttgaaggaaa tccatttttc
    12121 attctccttt tttgaaatga tttttatggt tttattataa gttatgtaag cggaaacaaa
    12181 aagaccaaat aaagcacagg cttgtgttca ctattggact atttccgaac tttaatggct
    12241 gatttaatca gaattcctcc ctatagttca ccagcccatt tattctaata atcaataaat
    12301 agacgttatt tcccctaaaa atttaaaaga acctcaaaaa acataaaaga agaggttggt
    12361 ttgaaaccaa cctcttctag agggaagtga tactctcaac tcttgtattt ataagacatt
    12421 cacaagattt tgagcaatgc tcctctggag agccaaatat tattactctt ctgatttttt
    12481 agttcttcct tgcgcagcaa ctgtcaaacc aagtccagca agtccgagac ctagaagtgc
    12541 caaatcgctt tgtcggtcac ccgtaatcgg cagactcgcc ttgcttgcac cacttgtcgc
    12601 ttgtccagat gcttgagcag tagtagaaag actcggtttt tcagcctctt tcttagcctc
    12661 catactgcct ttcacaacaa tacgtgtttc gctatcaaca actgtttctg ataacaaatt
    12721 agtagcaact accttaccat caatcaagac atcctgataa accagaactt tcttaccagt
    12781 tttgccttct tgaaggacat aagattgatc ggtagagaga cttgcatcat agcgaatttc
    12841 ctctacgata ggaagaattt tttcctctgt acgttccttg gtagtaaagt ctagacttgg
    12901 tttttcttca gcagtaggcg ctgtatctgg aactgccgta gtgatgtaag gcaagcgaat
    12961 caccgttgca gtcaacggtg caagcacaac tgcatcatct gtcaatgtca cacctaccaa
    13021 atcagtcagc gctgctattc cagcccgctt accatcgacc agcacgtgac ccttaagcaa
    13081 ttctttgtaa atatcagaaa gaacaaagct gcgctcctta ctgtcggcgt taacaaagac
    13141 agcatagcgg tccccattgc ttgcaatggt ttgataggca atgaccacat catttttggc
    13201 aatgccattt tgacctggaa ttgaaatcaa gctaaccttc tgttcaactt cttcttttgt
    13261 tcccaaacgg aaggcatctg tagatttgcg caaggcaatc aatccttttg taaaggcctg
    13321 agtacgggta ttctctggat agagcgcctc atctgtcgct ttggtccagt caaacttgtt
    13381 gaccgcatcc gatgaatcgt aagaatcatg aatgtagtat ggatagtcaa atggcgtacc
    13441 atctgcattt gtcaacaaat gagctttgtt cggtaccttg tcctctgaaa caggttcctt
    13501 gtaatcagga tggcggaact gtttggtccg tccatactcc tgaccagaat gaataaacgg
    13561 tgtcccttga gcagtcaaga ctagcaaatt acccaaacgt tgacgttggt gaatttcagt
    13621 gtaattttca gcaattgatg gatccttctt gatggactga gcgataatat caaagagggt
    13681 caagttatca tgcgcagcaa tatactggat cacatcacct ggatcatctg ccaagaagtt
    13741 gcctggttgt gccttgatgt tattgaagac agattctaca ctcttagcac cgcctgtgat
    13801 aaaggctggc tgaccttcgt ttggataacc agatttaagt gtattgcgaa tttcatctga
    13861 gaataccgca accgtatctg ttgaactcat ccagtcttgg tccgctggct gaacaggctg
    13921 attggcatca cctgtaaagg ttctccatcc ttcaccaagc ataatgatgt ttggattgag
    13981 tttttgtgcc tcggtaaagg ccagctcaat tgcttcggca tcatggtcac ccatcatatc
    14041 aaagcggaaa ccatctactt taaactcgtc cacaagatac ttgatagaat caacgaggac
    14101 acggcgactc atataatgag ttgttccgag acgaccacca ccaaagcttg atttagccgt
    14161 accatctgcc tccatgaagt gataatagtt tggctctaag tcttcaaata agaaggtctt
    14221 agaagtgtgg ttatagacca catccaaaat cacacccatg ccttgtttgt ggatttcatt
    14281 gaccaggttt ttgaattcct cgatgcgttt cattggatcg gtcggatctg tagaatacat
    14341 acctgtaaag gcaaagtagc tctgtggatc atagccccag ttgtagttgc tgttgctaga
    14401 agcatactca ctcatccgct ctgcattttt caattcattg acaaaatagt agctcataac
    14461 tggtagcaac tgaatatggg tcacacccaa gtctttgagg tagctgagac gctctgcaaa
    14521 ggccgcaaag gtaccgaact gagctgtcaa ttcatcagaa atagcaggat cggatgtgaa
    14581 gtcacggacg tgagcctcat agataatagc atcttcacgc tgattgaagt taggaattgt
    14641 ggcatagttc aaatcacttg gaccaacttc tgctgggtcc acaatcgcag cttttgcaat
    14701 ccgataagat ggatctgtat ctgccaaatc actattccac tccgctaaag atttagcata
    14761 tgggtctaaa acaagaacag attgaccccc gcgagtgatt tcataatggt agtagtaacc
    14821 acgatagtcc gcgataccaa gacctgattg actgttcaat tcgaggtccc attgtccctt
    14881 atcccccttg gtcatggcca atttaccgac caccttagat tggtcatcct tatcataaag
    14941 aacgactgcc acactgtctg cacttggaga ccatagggtc atatcaacga cactaccagc
    15001 ctgacgcaca cgcgcaccaa gctctccatc ataagcatag agctcatcct tcaactgcca
    15061 gctcatagtt gttgtgaagc tatcattgcc atatttgagg gtatagcccg cattttcttg
    15121 gttgaaatcc ccgaggactc gcaccttgtt gctggtaagg tccaagacaa gatccgtcac
    15181 agcaactgtc tgaccattct tatctgttac agacaatttc tccaagatgc tatccttatc
    15241 cgcaccttcc aatgttgtaa aggctgcttc taaggctgtt aggctaacgt gagaaacacc
    15301 tgtcacacgg acattattga caaaataagg gttggtgtaa atagtcgcat catcatcctt
    15361 gaggaagatt tgtgtctgat ttttcaaatc tttaaagcta taatctttct gctgaatctt
    15421 cacatcatct cctgacttgc tttcatccaa tagcaagaag ccaatggaac ttggtaaatc
    15481 tgtcaatttg acatcgatat aggcaccgta cttgccttgg ttttcaaaat caataccatt
    15541 tggccagtca cccaaggtaa ggtcagtcac atcaccccaa atccagagac ctttttggtc
    15601 ataattgcca tcggtacgga agtaattgat acggatgcag ccttctttta gaggttgata
    15661 gaggctgagt tggtagtttt catcaaacca agcttcattc atcttggtgc taatccgttc
    15721 aatggtcttg tcacctgtga tgttatcacc agaagtgtta ttaatcaaca gactaatctt
    15781 gctggctgtt tcatctttca tcttgatgtc cagataatag ccataatcat cctgcttagc
    15841 agtactgaaa ttggttgcac ctgttggcca agcaccaaca tctgacgatg gtttttcgac
    15901 atcatcccat gtccataaac ctaggctaga taaattatca gatggtagag ttttaaagtg
    15961 aacacggaag taacctgctt caattggttc ttcagttgct ccttgcacac ttgcattgac
    16021 gatgtcacct gcttctgtct gaaggagcat ggtgccacct tgaccgattg ttggaagttc
    16081 gacttgaaca acccatttac cacctttctc gatagcttga taagattttc ctgtgtaggc
    16141 atctgtgaca actacatctt tactagatac ttcaagcgcc atttccttgc tctcggtatt
    16201 ggttgagaag acaagatagg ctgcttgatc ttggtaactg cgtttgctga taagccaacc
    16261 ttggctatca tttccagcaa gggtgcttgt atcaccacgt gagagcaatg ttgaattggc
    16321 attgcggaaa gccaataatt tctgatagtg gtctgtgatg tcgctagtct ctaccttgct
    16381 ccaatcgaaa tcgtaccggt tgtcataaac tggccagttg ttttgcccag attgccccac
    16441 ttcttcaccg tagtaaatga ctggctgacc tttagctgta atcaaaagac tagcagccaa
    16501 tttcagtttg tccagatttc cttctttttc tgcgccaccg agactgtaga ggaaaccatc
    16561 ttcgtcgtga ctgcccaaga attgacccag agaagctgcg ctagtcaaaa ccttgctacg
    16621 ctcttctagc tcttttccag ctgctttcag ttgaccattg accaagtact tagcgatatc
    16681 cttgaagcca aagtccaaca agctgtccat agtaccgatt cccaagtcac ccttggtatc
    16741 tttatagtta gctccccatg tttctccaat taagtggaag tcaggatctc tatctaccaa
    16801 ttcatttttg aaatgttgcc atgtggtatc atcaacgtgt ttgactgtat cgacacggaa
    16861 agcatagata gagttgccct tggcagttgt tgagcgttcc agccaagatg cctgccaagc
    16921 aaccaactga ttgcgaacag cagcttcttc tgtcttgaag tccggtaatc cagataatga
    16981 gcctaattgg tcatctccct gcttgtcttc ttccttagtc cgtaccatac ctgcaaaggt
    17041 ttcttctgta ccatagccag catggttcag caccacgtca acaacaatat ttataccttt
    17101 ttccgccgca gcatcaatca attcgtggaa atcggctaaa cttcccaaat gcggatttag
    17161 tttctcaaaa ttggatgccc aataaccatg gtaagcatag tactctccat cttttacgct
    17221 accaacattc tgtggaatat tttccacaat cggagtcaac cagatagaat ccacacccaa
    17281 ttctttcaga tagtccagct tggctgtcac tcctttgaag tcaccgccct ggtaaagacc
    17341 gcgtgggttg gtgaccgttc cttctaccag cttatcattg ctggtgtctc cattatagaa
    17401 acggtctgtc atcatgaagt agatgacttg ctcgtcccag tctttttcac catctgcctt
    17461 gacacgctct gtaatggtca catccaattt tgtttcgtag taaccattgt ctttatcata
    17521 gacttttaca ggaagactat atgtacctgg cgctgtgcta gacgtcgctt taatggtcac
    17581 acgattgagc tcagtagaga ttggcacaag acctccgccg atggcacttg tatcaacttc
    17641 cattttgact atttcagcat tttcagggtt ggtgacagtt acagatacca agccgctttc
    17701 attgtagttg aatggtgctt tcacacttgc ctcaacagtc acattttttg tacccggagt
    17761 agctttgctt gtatctaatt ctactttttc ttcgataaag tatggatttg tgtaaacttt
    17821 gtcgtcaccg tttcgaagga aaatttggct atgccgttta cggtctgcaa aggctaaatc
    17881 aatggtttta ttgcctgcca aatctggatt attctgattg attagcagaa atccaagctt
    17941 ggaatctaaa ccattcgaga ggggaatgtc aacataagcg ccatatttac caagtttaaa
    18001 gtcaacggca cctgttggcc agccatcact cgcttcagtc gtatcgcccc aagtccaaac
    18061 gccccaacct tcataattat tgtcttcacg ggtgtagttg atacggagga cattttcatc
    18121 tttgagcggc tcatagctag acacgctata atcactagag atccaagcct cattttgttc
    18181 cggaacaaga agctccactt tttggtccga ttctgttatt ttttccccat tcttgagaac
    18241 cagataacca atcgttcctg gtgtctcaga ttttttaata tcaacgtaat ggccgtagtc
    18301 atctacctga ctgcttgaaa aattcgctgt atccgttggc cattgattac tatcggacgg
    18361 ctctgccact cctccccaag tccaaagtcc ttgactttct ggtgctgttt catctacatt
    18421 ttcaaagtgc agacgaatgc tgccttcttc gataggagct tcctctggag cagttgcatc
    18481 ggctgtttct gttaccgctg tcgcttcagc taccatagaa tctgtagctt ctatcgcagt
    18541 tgtggtcgct tctaccgcgc cgccatcctg ttccgaaaga gtcgttgaac tagttgacgc
    18601 agggaggaca acttcctctg cctgcactag ttgacctgct ccacttaaaa acagagcagc
    18661 cgcaatccca acagaggcta cacctacatt tagtttacga atgccaaaaa tctgtttctt
    18721 ctctagcatc cctgttcgtt tcatattctg aacggatttc tttttcatag actcctccat
    18781 ataaataagt ttgaattgta cactaccagt atatacaatt ccaaggattc tttgcaaacg
    18841 ttttcttaag tgttagcggt aacaagtttt gggccactat ctaacaaaaa tagaataaat
    18901 agctccccct tcttttgctc ctagaccgcc cactgaccaa aaaagagact actgaggtgt
    18961 agtctccctt tcaattaagg ttgcctggca gtaatgcccc tgtctagcca gaggcttgcc
    19021 atccaatatc gtcattaaat gtttgacgct aagagccccc atttgacgaa tcggctgctt
    19081 catcgttgtc aaggctggtc tggacatccg atctaaaaag aagccgtcaa aaccaatcac
    19141 cccaacgtca ttcggaatag actttcctag agcttgtaaa cctcttgtaa ccccaagagc
    19201 gagacgatcc gatgcgcaga taaaacaggt gttttctggt aaataatcaa gatttttcac
    19261 aaattcttcc gcaacacgag aacgattttc caatcgatag agatgagttt ctcggccctg
    19321 catcgcatct ttgtagcctt cttctctccc tttttcaaag gcttctggca agtcaattcc
    19381 tacaaaaaca atcctctcat aaccttttga taaagcaaac tgggttgcgg acctagtcgc
    19441 caaacgattg tctgaatcta caaaaggaag gccttcttga ttctccccga ataaaatcaa
    19501 tggcttccgc agccctttca accaagggta gtcattcttg cgagcaccag tcacaatgta
    19561 accatcacat tgatcagtaa tctgacttgg attggtcacc aactgcaagg tataatttcg
    19621 atgattgagc tcatcagcga ttcctgcaag aaggtcgata tagtacggct ctaccacatc
    19681 catcttttcg agaatgacag cctgaacaac cagtgtccgt tgctgggcca aggccttggc
    19741 cgccacattt ggcttatagt ttaattcttc catcgcagct gttacgagcg tccgcagctc
    19801 ttgtgtaact tgttctggat ggttgatgac acgcgaaaca gtcatctttg atacattggc
    19861 taacctagcc acatcggcta atgtcgtcat agaactcctc cctaattcta tttctgttgc
    19921 ttatctttta gcaagcaaac agtcaaaatt gtgcaaacgt tttctttagg ttattatata
    19981 ctatttatta ctaaaaagta aagacctttc gaatacattt atcgtcattt agttttcctt
    20041 ctattacttc cttagcttcc ttgcctaact tcatctatgc ctgcggctca tcgcctcgtg
    20101 ctaaaagtac tatataagaa tatccattcc ctctaaacaa aaaatactcc aaccgctaga
    20161 ccaatcaatg tctaacaatt gaagtatctt tgtcaaattc tgtccatttt ctgtacgatt
    20221 atggaaataa ataagataaa ttaatttgct tagcttactc aacatctacc ttttgaatgc
    20281 gagctaagaa ttgcttagta cgttcttctt gtggacggtc aaaaatgtct tctggtcgac
    20341 cttcttcgat aatccgacca ccatccataa atatgactcg atcagctacc gcacgcgcaa
    20401 agcccatttc atgagtcaca accaccatgg tcataccttc ttttgccaac tgcatcatga
    20461 cagccaagac ctcaccgatt aactcaggat ccaaggccga ggttggttca tcaaaataga
    20521 tgacctctgg attggtcgca atggctctgg caatgccgac ccgttgctgc tgacctcctg
    20581 atagctggct tggatagaaa ccatatttac cagatagtcc caccttgtcc aaggctgctt
    20641 taccgattct ttcagcctct tgtttgggta ctttccgagc cacaatcaga ccttccgtca
    20701 cattttgtaa ggcagtcttg ttggcaaaga gattatagtt ttgaaaaacg aaggctgtgc
    20761 gcttgcgaat gcctgcaatt tccttcttgg tcatctgagc caagtcatag ctgtcctcac
    20821 caaagactaa gtgacccgcg tcagcttttt ccaaaaagtt taaagaacga agaagagtcg
    20881 tttttcccga tccagacggg cctaaaatga cgaccacctc acctggattg acctctacat
    20941 caattccttt taacacctgc tgaccaccga aggtcagctt tactccactt gcttgaatca
    21001 tctgaacctc ctagtaaacg gctaacttct tctcaccata ggcttgaagc ttggtcaaaa
    21061 tggttgaaaa gagtagataa acaagggcca cctcacaata gagggcaaac ggttcgtagg
    21121 tccttgctgc aatctgctga gttgccataa acatctccaa aacggtaata ttggcagcca
    21181 gcgatgtatc cttgaccaat ccaatcaagg tgttggacaa gggcgggaag gccactcgaa
    21241 aggcctgtgg gagaataatc cgacgcatgg tctgggcaaa actcatcccg acagaatagc
    21301 cggcctccaa ctgcccctta ggcacggact cgatagatgc ccgaatggtt tccgcagcgt
    21361 aggcacctgt attgacagaa aaaaccaaaa ccgccgacgg aaaggcatcc aagacaattc
    21421 ctagacttgg tagcccaaag aatatcacat agagttgaac caagagtggt gtccctcgaa
    21481 caacccaaat atagaaacga gccacttgtt tgagaatggg aatctgagca atttgcacca
    21541 aggccgtccc aatagcaatt agcaagccaa aggtaaaaga caggattgtc agtgggatag
    21601 tcaccaacaa tcctggaaca agtatctgtg aaaatgaatc aagcaatagt tgccatactc
    21661 tttctgtcat tgtttctcct gattacttag cttttgatac gtcttcacca aagtatttgt
    21721 ttgaaatttc aactaatttt ccttcactag ccaattctgc caaggcggca tcaatctctt
    21781 tcgcaagatc ttcatttcct tttgcaactg ggaaggctgt tgtcgatacg tcatccgtca
    21841 aggtcacaac cttaatcggt gcatcagcat gctgtttcac ataatccaag tacaccaagt
    21901 tgtcattgat ggtagcatcc acacgccctg tatttaacaa ttcaactgcc tgagagaatc
    21961 catccacacc gacaacttct gcaccatttt cacgcgccaa atctgcccag ttacttgtca
    22021 agctgttggc tgatttttta ccagataaat ctgcaaagct tgtaatgtca gtattatcct
    22081 tctgtgttac caaggctcca tagatgtatg tatatggtgt tgaaaaatcg tattttgcct
    22141 tacgctcatc tgtaataccg acttggttag cgatggtatc aaaacgggcc gcatccaaac
    22201 ctgcaatcat ggaatcccat tctgtttcaa caaattccac cttaacaccc aatttctcag
    22261 caacggcttc ggctacttct acatcataac cgaccaattt cccgctctca tcgtggtaag
    22321 agtaaggagc ataggcacct tcagtaccga tttgaataac gccttcttcc ttaatttgtg
    22381 ccaagagggt ttggtctgta ctattcgata tacctgcaga tgaattgcag gctgctaaca
    22441 cgatacccgc aaacaataaa cttgtaaaca tagctatttt cttcataatt
//
LOCUS       DBNFEG010000013        21942 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.013, whole genome shotgun sequence.
ACCESSION   DBNFEG010000013 DBNFEG010000000
VERSION     DBNFEG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21942
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.013"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            1..504
                     /locus_tag="AC1PNG_000339"
     CDS             1..504
                     /locus_tag="AC1PNG_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700876.1"
                     /translation="MTGIVAACVRYFFYFMIALYAFITLVGLGGHSFVIDSWEYTFNK
                     PIDQINIWLILLTTGLILIVLAALSHIAHLIQKLCKLLLEEDYFASSSLALYQKLLVS
                     LLILTAGQFCLTTLFFLTHATNPNTFLNLRWSDFILNALFLFATYFVWTLVQKGQQLE
                     TENSEFI"
     gene            506..721
                     /locus_tag="AC1PNG_000340"
     CDS             506..721
                     /locus_tag="AC1PNG_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6700877.1"
                     /translation="MEMIRVNLDKVLKDRQMTSKDLAEQVGITEANLSILKTGKAKGI
                     RFNTLMSICRILDCQPGDILEYVREGE"
     gene            723..1229
                     /locus_tag="AC1PNG_000341"
     CDS             723..1229
                     /locus_tag="AC1PNG_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HIP6700878.1"
                     /translation="MNKKIFFNIVIQLLTFIRYGLIALTGLIMLALLILLIGGKPIQN
                     SLRLDVPLPIIVIILIISILLVACFIYMATILKKLLINFQTGTIFSTENVVYTKIILG
                     TLIVWTSIQFASAFFLSYLDLKNVSDLYDFSLSDYFINGVLIIIALLAKMVLEKGVLL
                     QAENDEII"
     gene            1241..1711
                     /locus_tag="AC1PNG_000342"
     CDS             1241..1711
                     /locus_tag="AC1PNG_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700879.1"
                     /translation="MKKYISLIALCMMIPCLSACHSVTSEVNKEIQNELTAASIETEK
                     ALKTDERLAKIDNFQQIVEITTSKGEFSLESPFSGKIYLMATKENLEKLSKEEFTLNE
                     KSFSTIDAGSSSDFNIRYKHQTYFTIQSLSVQDLEDFEYTKSSSTSAILVFYPD"
     gene            complement(1749..2420)
                     /locus_tag="AC1PNG_000343"
     CDS             complement(1749..2420)
                     /locus_tag="AC1PNG_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIP6700880.1"
                     /translation="MDINWQAVFNADIAREAIPQILEGLPYTLSLSLIGFALGTFCGF
                     FVALMRMSKFGPLHWLAMAHISLMRGIPLMVLLFFIYFGLPFMGLQLDAISASIIAFT
                     SMSSAYISEIIRASLSAIDKGQWEAARSLGLRTNVIYRKIIIPQAFRIALPPLSNVLL
                     DMVKSTSLTAMITVPEIFNKAKIVGGAKSDYMTVYICVALIYWAICTLYAFGQLRLEK
                     RLATY"
     gene            complement(2511..3386)
                     /locus_tag="AC1PNG_000344"
     CDS             complement(2511..3386)
                     /locus_tag="AC1PNG_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIP6700881.1"
                     /translation="MLKKVLASALLVSTLTLAACSSSSYNTSSSSAEQTQWEKVKETG
                     VLKVATPGTLFPTSYYNDAKELVGYEIDMINEIGKRLKLEIEYQEIGVAEAFTAVDSG
                     KVDISVNNFDMTEARKEKYNFSIPYKYSVGGYIVREDGSSGIEAADLSDWTGKKAGGG
                     AGTQYMKIAEKQGAEPVIYDNVTNDVYLRDVSTGRTDFIPNDYYTQVMAIKWANANFP
                     DIKVKMGEAKYNPTEQGIVMSKADTSLKEKIDEALEAMKADGTLKAISEKYYAGQDLT
                     VPVENADQLPVIEVE"
     gene            complement(3510..4958)
                     /locus_tag="AC1PNG_000345"
     CDS             complement(3510..4958)
                     /locus_tag="AC1PNG_000345"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIP6700882.1"
                     /translation="MTWKDATAMAQAVNQKQVSAKELVQETIDRIEKLNPTLNAVVSK
                     QYEEALKEAEKEDYLGKPFAGVPFLLKDLGQNEKGQPSSAGSRLLAGRPAGHTDTYVQ
                     RLKDLGFIIVGRTNTPEFGFKNISDASLHGPVKLPLDPSRNAGGSSGGAAAALASGMV
                     SIAAASDGGGSIRIPASFNGLIGLKTSRGRIPVGPKSYRGWQGASVNFALTKSVRDTQ
                     RLLYHFQDYQLEAPFPLAKLSEEEVFGKLSRPLKIAYYTKSPVGSKVSPEAVEAVQKA
                     CHHLANLGHEVVELSEYPLDGVALMKSFYLMNSVETAQMFDEIEAGLGRPMTPDDMEV
                     MTWAIYQSGQTIPAKLYSKVLQDWDRYSAIMADFHQHYDLLLTPTVADVAPKLDQFTH
                     SQEMLDRLLHMQELAMAEQQSLIWEMFADSLAWTPFTQQANITGQPAISLPTYKTPQG
                     LPIGIQLTAAKGREDLLLQLADQMEAAGLLNL"
     gene            complement(4968..5795)
                     /locus_tag="AC1PNG_000346"
     CDS             complement(4968..5795)
                     /locus_tag="AC1PNG_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HIP6700883.1"
                     /translation="MKSWVENVTIKKYEDRSGLTGLKQGPLVWTEVFLREVNRQSNTG
                     ILHFWPMDQTVILGMMDSQVAQLDKGLASIGQAGYSPIIRSLGGLAVVADDGILNVTL
                     IFPNPSGHKVDLRESYQVMVDLIAQALSDFPFEVVSGEVATSYCPGTYDLSIKGRKFA
                     GLAQRIYQEAIAISAYISVSGNQVKRGQVVADFYAASFAPQEVSDRFPQVNPNSMANL
                     SDLVAQDLTMEDMKIRIEHVLIENGAFLTTFYPSSDNMADFMALGKTIKQSMEKYGI"
     gene            complement(6071..7207)
                     /gene="dnaJ"
                     /locus_tag="AC1PNG_000347"
     CDS             complement(6071..7207)
                     /gene="dnaJ"
                     /locus_tag="AC1PNG_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIP6700884.1"
                     /translation="MNNTEFYDRLGVSKNASPDEIKKAYRKLSKKYHPDINKDPGAED
                     KYKEVQEAYETLSDPQKRSAYDQYGPAGANGGFGGGAGGFGGFDGAGFGGFEDIFSSF
                     FGGGGATRNPNAPRQGDDLQYAVNLKFEEAIFGVEREVSYNREATCRTCTGSGAKPGT
                     SPVTCGRCHGSGVINVDTQTPLGTMRRQMTCDVCHGRGKQIKDPCATCHGTGHEKQAH
                     TVTVKVPAGVETGQRIRLAGQGEAGFNGGPYGDLYVVIQVQPSDKFEREGTTIHYKLN
                     LNFVQAALGDTVHVPTVHGDVDMVIPEGTQTGKTFRLKGKGAPSVRGGAIGDQYVTVN
                     IVTPTGLNDRQKAALKEFAAAGNIDVKPHKKGFFDKVKDAFEDL"
     gene            complement(7741..9564)
                     /gene="dnaK"
                     /locus_tag="AC1PNG_000348"
     CDS             complement(7741..9564)
                     /gene="dnaK"
                     /locus_tag="AC1PNG_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIP6700885.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTIISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     EYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDHMVAEFKKEN
                     GIDLSVDKMALQRLKDAAEKAKKDLSGVTSTQISLPFITAGAAGPLHLEMTLTRAKFD
                     ELTYDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRND
                     VDQAIFATEKTLKETEGKGFDAERDQAQAALDELKAAQEANNLDDMKAKLENLNEKAQ
                     ALAVKLYEQAAAAQQAAAGQEGAQTANNAGDDVVDGEFTEK"
     gene            complement(9653..10189)
                     /gene="grpE"
                     /locus_tag="AC1PNG_000349"
     CDS             complement(9653..10189)
                     /gene="grpE"
                     /locus_tag="AC1PNG_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIP6700886.1"
                     /translation="MENEEVNNLSEEIKNEEIVEEVEATEEVVETPEKSELDLANERA
                     EEFENKYLRAHAEMQNIQRRANEERQTIQRYRSQDLAKKILPSLDNLERALQVEGLTE
                     DVKKGLEMVQESLIQALKEEGVEEVATDVFDPNLHMAIQTVPATDDCPAEHIAQVFQK
                     GYKLHERLLRPAMVVVSE"
     gene            complement(10302..11336)
                     /gene="hrcA"
                     /locus_tag="AC1PNG_000350"
     CDS             complement(10302..11336)
                     /gene="hrcA"
                     /locus_tag="AC1PNG_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIP6700887.1"
                     /translation="MITQRQNDILNLIVELFTRHHEPVGSKALQEMIASSSATIRNDM
                     AKLEQLGLLEKAHTSSGRMPSRAGFQYFVNHSLNLEHIDEEDVYQVVKAFDFEAFKLE
                     DILERASTVLADLTGYTSVILDVEPTSQQLTSFDIVQLSSHDALAVLTLDQSKPVTVQ
                     FAIPKNFLTRDLEVLKRLVDERFVDQTVLSIHYKLRTEIPQVVQRYFATTDNVLDLMD
                     YIFSNLFQESVFISGKVASLTYGNLATYQLLDSAQLLAPELRQGLAPDQQTSISVAEH
                     SDSALADVTVIHHRFPIPYRGMGQMSLLGPVDMNYRRQMSLINIISRVLFMKLTDYYR
                     YLSSNHYEVN"
     gene            complement(11558..12253)
                     /locus_tag="AC1PNG_000351"
     CDS             complement(11558..12253)
                     /locus_tag="AC1PNG_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIP6700888.1"
                     /translation="MADVRLYISRHGKTMFNTIGRVQGWCDTPLTKAGEEGIRELGLG
                     LKDAGLEFKLAVSSDLGRTVQTMTIAQRELGILGKIPYYQDKRIREWCFGSFEGMYDA
                     ELFQGVLPRLKGTVDATGMTFAEIAAGIQEADTAGWAESWEVLSNRILTGFEAIAQDL
                     KKQGGGNALVVSHGMTIATLAHLLEPERGANIFLDNGSITVLKYENGKLLIEAVGDLS
                     YRKRGAELIAQGK"
     gene            complement(12329..13666)
                     /locus_tag="AC1PNG_000352"
     CDS             complement(12329..13666)
                     /locus_tag="AC1PNG_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HIP6700889.1"
                     /translation="MDIRQVRETIEMIEEQNFDIRTITMGISLLDCIDADIEKAAEKV
                     YTKIVTKAKNLVAVGDEIAAELGIPVVNKRVSVTPIALIGAATDATDYLPLAHALDKA
                     AHEIGIDFIGGFSALVQKGYQKGDEILINSIPQALAQTTKVCSSVNIGSTKTGINMTA
                     VRDMGRIIKETAEASDMGAAKLVVFANAVEDNPFMAGAFHGVGEADVVINVGVSGPGV
                     VKRALEKVRGESFDVVAETVKKTAFKITRIGQLVGNMASERLGVKFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDAVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPETTPAETIAAMIADEAAIGVINQKT
                     TAVRIIPLGKEGDMIEFGGLLGTAPVMKVNQASSADFINRGGQIPAPIHSFKN"
     gene            complement(13678..13944)
                     /locus_tag="AC1PNG_000353"
     CDS             complement(13678..13944)
                     /locus_tag="AC1PNG_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIP6700890.1"
                     /translation="MKAIVTVVGKDKSGIVAGVATKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKKDFTKLRAELEAFGQGLNVKINIQSAAIFDAMHNL"
     gene            14201..15943
                     /locus_tag="AC1PNG_000354"
     CDS             14201..15943
                     /locus_tag="AC1PNG_000354"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6700891.1"
                     /translation="MKIIKQLWWFFSLEKKAYLIGILSLCLVSLLNLLPASIMGQLID
                     QIASGQLTEHRLLLGVAGLILSALAMYGLRYLWRMNILATSYRLGKIMRARLFNHFMH
                     LSPSFFQQYRTGDLMAHATNDINALTRLAGGGVMSFVDATVTALVTLVTMSVSISWQM
                     TLVAILPLPFMTLTTNFLGRRTHENFKASQAAFSELNNKVQEAVSGIKVTKSFGYQEQ
                     ETAAFQEVNQAAFLQNIKTMRYDALFNPAVLFFIGLSYLLTLLVGSHFISQGQVSLGQ
                     LVTFMTYLDLLVWPLMAIGFLVNISQRGDVSYNRIQTLLSISSEVIETDQPLPAPSNG
                     EIVYEISEFAYDNIPVLQDIQFHIKKGQTIGLVGPTGSGKTSLLKLLMREYDVTNGQI
                     LLNQQNIKHYKLGDLRQLMGYVPQDQFLFATSVAENIRFGNPDLSLDQVKEAAKAVHV
                     YEDIQDMPDRFDTMVGEKGISLSGGQKQRLAMARAMILNPDILLLDDSLSAVDAKTEH
                     AILETIKQERLDKTTIITAHRLSAIVHADLILVLEDGKIVERGRHQELLDEKGWYYDT
                     YMMQQLEKEESDAL"
     gene            15933..17681
                     /locus_tag="AC1PNG_000355"
     CDS             15933..17681
                     /locus_tag="AC1PNG_000355"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6700892.1"
                     /translation="MLSKTKQASVFWRLLSYLKAYRLLTVLALIFLLLTTVVRSLIPL
                     LASYFIDHYISSISETAAAILAAYFALYLLQMVLQYFGNLWFAKVSYSIVRDIRRDAF
                     GRMEKLGMAYFDQTPSGSIVSRITNDTESISEMFSGILSSFISALFIIITTLSTMFAL
                     DWKLTCLIVLFLPLIFLLVDQYRKRSAPIVAKTRSLLSAINSKLAESIEGIRIIQAFS
                     QEERLKEEFEAINQEHLDYASRSMALDSLFLRPALSLVKILAYALLMTYFGLDWSQAG
                     ISAGLIYAFIQYVNRLFDPLLDVTQHFSTLQTSMVSAGRVFTLMDQTFEEPLQLDRGA
                     KIDKGHIVFEDVCFSYDGKRQILDKVSFEVRQGQTIAFVGATGSGKSSIINVFMRFYE
                     FQSGRILIDGQDIRSFSQEELRSAIGLVLQDPFLFHGTIASNIQMYQDISREEIEEAA
                     RFVDAAPFIEKLPEKYDHLVTERGTAFSTGQRQLLAFARTMASQPKILILDEATANID
                     SETEQTVQTSLEKMRKGRTTIAIAHRLSTIQDADCIYVLDKGKIIESGNHEELLALKG
                     HYHRMYQLQAGQLTAT"
     gene            complement(17776..18873)
                     /gene="fni"
                     /locus_tag="AC1PNG_000356"
     CDS             complement(17776..18873)
                     /gene="fni"
                     /locus_tag="AC1PNG_000356"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HIP6700893.1"
                     /translation="MFYLGEFGLDRKDQHVGLANQQYSATPAKDFTETLFVHHSLPQT
                     KVDEVDISTSVAGLDFAFPFFINAMTGGSKKTREINRLLGIMGHFGKIALASGSVSAA
                     IKDPSVAETFSVMRRENPYGIIFANLGAHHSVENAKRAVDLLEANAIQIHVNAPQEIV
                     MPEGDRDFTMWLKNIETLVREMEVPVIVKEVGFGMSRETVAQLASVGVQTIDVSGTGG
                     TDFAKIENARRTFNDYTYLEGWGQSTVTSLVEAMSVSEEVRPSLIASGGIKTPLDIVK
                     SLALGADLVGMSNHFLQYVKDGKGHRFDDGLQAIKTYQWQMAEIMTMLGAKNIAELRQ
                     KDLVLAPNIQNWCEARGIDWKAYARRSANVS"
     gene            complement(18884..19963)
                     /locus_tag="AC1PNG_000357"
     CDS             complement(18884..19963)
                     /locus_tag="AC1PNG_000357"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HIP6700894.1"
                     /translation="MKVQVKIPGKLFLAGEYAVVEAGYPAVIAAVDQYLTVTIETSDR
                     GLLHSSQQVDLYLIWERKEGIAHVQGDHPYALIETVMQVTEAYLTAKGYACHGTYSLA
                     VQSDLDDQTSGAKYGLGSSGAVTVATVKALLTYYGHRPDALLTYKLAALAQTKLGMTG
                     SFGDLAASSFGGLIAYRSLDRSWLLGKMAELSLLDLVESDWQSLSISPIQLPQGLDLL
                     VGWTGSAASTDSLVSQMESQKSQIEKEQIHVQFLADSKICVEQLIVACQTNDSASVRQ
                     ALTQNRKLLQDFARGMGLVIETPQLSQLCDLAQTYGAVAKSSGAGGGDCGICLVDSKE
                     QKAAIETAWQEAGIFPLTLNIASRE"
     gene            complement(19965..20990)
                     /gene="mvaD"
                     /locus_tag="AC1PNG_000358"
     CDS             complement(19965..20990)
                     /gene="mvaD"
                     /locus_tag="AC1PNG_000358"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HIP6700895.1"
                     /translation="MTKQTGIARAHTNIALIKYWGKRDKELFLPMNSSLSLTLDAFYT
                     DTKVVFDPELTADEFYLNGMLQKEKEILKISRFLDLFCEYIGERAFARVESLNFVPTA
                     AGLASSASAFAALALATATALDLDLSPATLSTLARRGSGSSTRSLFGGFVEWDMGTGS
                     EDSMAHPIDDADWDIGMVVLAVNTGPKKIASREGMDHTVATSPFYTAWVETAKQDLVD
                     IKAAIASRDFEKLGQITEHNGMKMHATTLSANPPFTYWSADSLVAQEAVRQVREATGL
                     SAYMTMDAGPNVKVLCRTSQMDELVAELAKVFPREKIITSKPGPAAYVLSEDEWQTSQ
                     AAFEKGL"
     gene            complement(20983..21918)
                     /gene="mvk"
                     /locus_tag="AC1PNG_000359"
     CDS             complement(20983..21918)
                     /gene="mvk"
                     /locus_tag="AC1PNG_000359"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HIP6700896.1"
                     /translation="MSVVGKANGKIILMGEHAVVYGQPAIAMPFSAVEIIAQVTAQGE
                     ALTVACDFYKGLVHKMPKIWESLKHAIRFSLYRIGAPTDPAIHIEISSTIPAERGMGS
                     SAAVAVAVARALFAYYKKELTDSELWDIVQSSEKIAHGNPSGIDAATTSGKSPVFFIK
                     HQPIEPFELKFHAHLVVADTGVTGNTLEAISDVADLLEKKPEAIKLVEELGNLTRQAK
                     EDLATDQAELLGSRMNQAHALLQKLGVSDPSLDKLVSLAQENGALGAKLTGGGRGGCM
                     IALARTAQDAQKLAHILDQAGARQTWIQYLGETND"
BASE COUNT         5666 a         5405 c         4717 g         6154 t
ORIGIN      
        1 ataactggca ttgtggctgc ttgtgttcgc tatttcttct atttcatgat tgctctctat
       61 gccttcatta ccttggtagg tcttggaggc cactcctttg tgatagactc ttgggaatac
      121 acctttaaca aacccattga ccagattaac atctggctca ttctattaac gactggattg
      181 atcttaattg tcttggcggc actcagtcat attgcccacc tgatccaaaa gctatgtaag
      241 ctacttctgg aagaagatta tttcgcctca tctagcctag cactctatca gaaactcttg
      301 gtcagtttac taattttgac tgctggtcaa ttttgcctaa ccactttgtt tttcctgaca
      361 catgctacta atccaaacac cttcctcaat cttcgctggt cagactttat cctcaatgcc
      421 ctcttcctct tcgcaactta ttttgtctgg accttagtac aaaaggggca acaacttgaa
      481 accgagaaca gcgagtttat ctgatatgga aatgattcgc gtaaacctag acaaggtcct
      541 caaagatcga caaatgactt ccaaggactt ggcggagcaa gtcggcatta ccgaagccaa
      601 cctgtccatt ctcaagacag gcaaggcaaa aggcattcgt tttaatacct tgatgagcat
      661 ctgccgcatc ctagactgcc aaccaggaga tattttggaa tatgtgaggg agggggaata
      721 gcatgaacaa aaagatattt ttcaacatcg tcatacagtt gctaacgttt attcgttatg
      781 gattgattgc cttaacggga ctgataatgt tggcacttct tattctactt ataggcggga
      841 aacctattca aaatagtcta cgattagatg tccccttgcc tatcattgtt attattctaa
      901 tcataagtat cctattagta gcctgcttta tctacatggc caccatactc aaaaaattgc
      961 tcatcaactt tcaaacagga actattttct caactgaaaa tgtagtctat acaaagataa
     1021 ttttaggaac cttaatcgta tggacgagca tacaatttgc aagtgctttt ttcctatctt
     1081 acctagattt aaagaatgta agtgatttat atgatttttc acttagtgac tacttcataa
     1141 atggtgtgct gattattatt gcactactag caaagatggt gttggaaaaa ggtgtgcttt
     1201 tacaagctga aaatgatgaa attatataaa ggagcccatg atgaaaaagt atatttccct
     1261 cattgcactc tgcatgatga taccttgctt aagtgcttgt cactctgtca cttcagaagt
     1321 taataaagaa atacaaaatg agctaacagc agcgtccatt gaaacagaaa aagctctaaa
     1381 aacagatgaa cgtctggcta agattgataa ctttcagcaa attgttgaaa tcacgacatc
     1441 caaaggagaa ttctctcttg aatctccttt ctctggcaaa atctatttaa tggcaacaaa
     1501 agaaaatctc gagaaactct caaaagaaga gtttactctg aatgaaaaga gcttctcaac
     1561 tatcgatgct ggtagtagca gcgattttaa tatccgctac aaacatcaaa cttacttcac
     1621 tattcaaagc ttgtccgtgc aagatctgga ggatttcgaa tacaccaaat cctcttccac
     1681 ttctgctatt cttgtcttct atccagacta gcaaaaattc ctcgattcaa aagaaccgag
     1741 gaattttttt aatacgtcgc caagcgtttt tccaacctga gctgaccaaa tgcataaagg
     1801 gtacagatag cccaatatat caaggcaaca cagatataaa ctgtcatgta atccgacttg
     1861 gctccgccga ctatttttgc cttattgaaa atctctggca ctgtaatcat agcggtcaag
     1921 gacgtacttt tcaccatgtc caagaggaca ttgctcagcg gtggaagggc aatccgaaag
     1981 gcttggggaa taatgatttt tctgtaaatc acattggtcc gcaagcccag ggaacgcgcc
     2041 gcctcccatt gtcccttatc aatggccgaa agagatgcac gaataatttc agaaatgtag
     2101 gcactggaca tggatgtaaa ggcgataata gatgcggaga tggcatctag ttgtagcccc
     2161 atgaaaggca aaccaaaata aatgaagaaa agaagaacca tgaggggaat tccacgcatg
     2221 agagaaatat gggccatagc caaccaatgc aagggtccaa acttagacat ccgcatgagg
     2281 gctacaaaaa atccacagaa agtccccaag gcaaaaccaa ttagggatag ggatagagtg
     2341 tagggaagtc cctccaaaat ctgtggaata gcctctcttg caatatctgc attaaaaact
     2401 gcctgccagt taatatccat atcttctcct ctttcctaaa aaccagatag accttgccag
     2461 cctgtgactg acaaagtcta tacaagatgc atttaatttg ttacaaggtc ttattcaact
     2521 tcgataactg gcaattggtc tgcattttca acagggactg tcaagtcttg tcctgcgtag
     2581 tatttctcag aaattgcctt caaagttccg tcagctttca tcgcttcaag agcttcgtca
     2641 attttttctt tcaagcttgt gtcagccttg ctcataacaa tcccttgttc agttggattg
     2701 tattttgctt cacccatttt taccttgatg tctgggaagt tagcatttgc ccacttgata
     2761 gccattactt gtgtgtagta gtcgtttgga atgaagtctg tacgacctgt tgatacgtca
     2821 cgcaaataga catcgttggt tacgttgtca tagataactg gctctgcacc ttgcttttca
     2881 gcaatcttca tgtactgagt acctgcgcca ccaccagctt ttttaccagt ccagtcgctc
     2941 aaatctgcag cttcgattcc tgatgaacca tcttcacgga cgatgtagcc accaactgaa
     3001 tacttgtaag ggatagagaa gttgtacttt tccttacgtg cttctgtcat gtcaaagttg
     3061 ttaacagaaa tatcaacttt tccactgtcc acagctgtaa aggcttctgc tacaccgatt
     3121 tcttggtatt caatttccag tttcaaacgc ttaccaattt cattgatcat gtcaatttca
     3181 taaccgacca attctttggc atcgttgtag taagaagttg ggaagagggt tcctggtgtt
     3241 gcaactttca agacacctgt ttctttcact ttttcccact gggtctgttc agcagaagaa
     3301 ctgctagtat tataacttga actagaacat gctgccaagg tcaaagtaga aaccaagaga
     3361 gctgaggcca aaactttttt caacataaaa caatctcctt tgaaagctat aacttccttt
     3421 ttactttttc ggtgttaacc caccaacttt tacaggttaa cattttgaaa acgttttggc
     3481 aaataattga actgaaaaaa tgaattccct tacaagttga gtaaacccgc tgcctccatt
     3541 tggtctgcca actgcaagag taaatcctct cgtcccttgg ctgctgtcaa ttggatgccg
     3601 attggtaagc cttgtggggt cttataagtt ggcaagctga tagccggttg gccggtgatg
     3661 tttgcctgct gggtaaaggg tgtccaggct agactatctg caaacatctc ccaaatcaaa
     3721 ctctgctgct ctgccattgc caactcctgc atatgcaaca agcgatccag catctcttgc
     3781 gagtgagtga actggtctag cttgggagcc acatctgcga ctgtcggcgt cagaaggaga
     3841 tcgtagtgct ggtggaagtc cgccatgatg gcactgtagc ggtcccaatc ctgtaagact
     3901 ttggaataga gcttggctgg gatggtctgc ccgctctggt aaatagccca ggtcatgacc
     3961 tccatgtcat caggggtcat gggacgaccc agccccgcct caatctcatc aaacatctgg
     4021 gctgtttcaa cgctattcat caggtagaag gatttcatca gggccactcc atccagagga
     4081 tattctgata attccacaac ttcatgcccc aaatttgcca agtgatgaca ggctttttga
     4141 accgcttcta ccgcttcagg actgaccttg ctccctactg gagatttggt atagtaggcg
     4201 atttttaggg ggcgagacaa ctttccaaac acctcttctt cgctcagctt ggcaagggga
     4261 aagggggctt ccagctggta gtcctgaaaa tgatagagca gacgctgggt gtcacggaca
     4321 gacttggtca gggcaaaatt gacagatgct ccctgccagc cacgataaga cttaggtcca
     4381 actggaatcc gaccacgact ggttttgagc ccgatcaagc cattgaagga ggctgggatg
     4441 cgaatagacc caccaccatc cgatgccgca gcaatagaga ccatgcctga agccagtgct
     4501 gcagctgcac caccacttga tccaccagca ttgcggctag gatctaaggg aagcttgaca
     4561 ggaccgtgca ggctggcatc tgagatattt ttgaagccga attctggcgt gttggttcga
     4621 ccgacaatga tgaaacccaa gtccttcaag cgttggacat aggtatcggt atggcctgct
     4681 ggtcgaccag caagcaggcg agaaccagca gaagatggct gccccttttc attttgcccc
     4741 agatccttca acaagaaagg aactccagca aatggcttgc caaggtagtc ttctttttca
     4801 gcttctttca aggcctcttc atactgcttg ctgacaaccg cattgagggt gggattgagc
     4861 ttttcaatcc tgtcaatggt ttcttgcacc aattccttgg cagaaacctg tttttgattg
     4921 acagcctgag ccatggctgt cgcatctttc catgtcataa gaatctccta gataccatat
     4981 ttttccatag actgcttgat ggtctttccg agcgccataa aatcagccat gttgtccgac
     5041 gaaggataaa aggtggtcag aaaagcccca ttttcaatca acacatgctc gattcgtatt
     5101 ttcatatcct ccatcgtcaa atcctgagcg accaagtctg acagattggc catcgaattc
     5161 ggatttactt gcggaaatct gtccgatact tcttgtggag caaaacttgc cgcataaaaa
     5221 tcagccacca cttgcccacg tttgacttga ttgcctgata cactgatata ggcagaaatg
     5281 gcaatagcct cttggtaaat ccgctgagcc aatccagcaa acttccgccc cttaatacta
     5341 aggtcgtagg ttccagggca gtaggaagtt gccacctcac cactgaccac ctcgaaggga
     5401 aaatctgaaa gcgcctgggc aatcaaatcc accatgacct gataagactc ccgcaaatca
     5461 accttgtgac cagaaggatt gggaaaaatc agagtgacat ttaaaatccc gtcatccgca
     5521 acgactgcca aaccgcccaa actccgaata ataggactgt agcctgcctg accaatactg
     5581 gctaagccct tgtccagctg ggcaacctga ctgtccatca tccccagaat gaccgtttgg
     5641 tccatgggcc aaaaatgtaa aatccctgta ttggactgac gattgacctc gcgcaaaaag
     5701 acctcagtcc acaccagtgg cccctgtttc aaacccgtca aaccagaccg atcttcgtat
     5761 ttctttatag tcacattctc aacccaagat ttcattcgta agcgttctct ttcaaattac
     5821 tatatcttat tttaacacag agcctgtgat gcgtcggaaa aaatgcccag aggattttct
     5881 gaaaaagaaa ggggagtggg aaagaactcg accaagataa aaagagttcg tcttcccacc
     5941 cccgcacagt tgattaggcc agatttggag tgcaaaacac gaacaaatct gccaatcaac
     6001 cactgcgctg agatgttgaa acgaactctg agtagtggtc ctgggctttt tgcccagtct
     6061 catcttagaa ttataaatcc tcaaaggcat cttttacctt gtcaaaaaat ccttttttat
     6121 ggggtttgac atcaatattt ccagctgccg caaattcttt aagagctgct ttttggcggt
     6181 cattcaaacc tgtcggagtg acgatattta ctgtgacata ttgatcacca atagctccac
     6241 cacgaacact tggtgcacct tttcctttga gtcggaaggt cttaccagtc tgagtacctt
     6301 caggaatgac catatccaca tcaccgtgaa ctgttggcac atgaactgta tctccaagtg
     6361 ctgcttggac aaagttgagg ttcagtttat agtgaatagt ggttccttca cgttcaaact
     6421 tgtctgaagg ttggacctga atgacaacgt agagatctcc gtatggacct ccattgaaac
     6481 cagcttcacc ttgacctgcc aagcgaatac gttgaccagt ctcaacacca gctggcacct
     6541 taaccgttac cgtatgggct tgtttttcat gaccagttcc atgacaggtt gcacatggat
     6601 ctttaatttg ttttccgcga ccatgacaga catcacaggt catttgacga cgcatggttc
     6661 caagtggagt ttgggtgtcc acattgatca ctccagaacc atgacaacgc ccacaggtta
     6721 ccggacttgt tccaggtttg gcaccggaac ctgtacaggt tcgacaagtt gcctcacggt
     6781 tataggaaac ttcacgttcc acaccaaaaa tcgcttcttc aaatttcaaa ttgaccgcat
     6841 attgcaagtc atccccttga cgaggtgcat taggattgcg cgtcgcacca cctccaccaa
     6901 agaagctaga gaaaatgtct tcaaagccac cgaaacctgc tccgtcgaaa ccaccaaagc
     6961 cacctgctcc tccgccgaag cctccattgg caccagcagg accatactgg tcataggcag
     7021 aacgtttttg agggtcgctc agagtctcat aagcctcctg gacctccttg tatttatcct
     7081 ccgcacccgg atccttatta atatctggat ggtatttttt cgaaagcttc cgataagcct
     7141 tcttaatctc gtctggcgaa gcattcttag aaacccccag acgatcgtaa aattcagtat
     7201 tgttcatata agatactaag ggcgtaaagc ggacacagcc aaaataggag ttttgacgca
     7261 ggacctcacc gtcctaggaa aaactatctt tttggcacag tccgtagccc gtgttcgatt
     7321 acgaacactt tcctttctat catagttttg gaaaagtgaa aataggaaac tgacgcagtg
     7381 tgtaaaacac actaggaggt ttatcttttt cactaggttt ttagcctgtg ttcgaataaa
     7441 cgaacttgta ttcctttctt tgaaaattag aaagtagccc gtgttcagtt acaaacactt
     7501 tcctttctgt aaaagttaga agggtattta cccaaaaaca gaggctgggt tgcaacctct
     7561 gtgattggaa attttattga cgaatgattt ctattctcag gaagataaga attgagttcg
     7621 agctaagaac cttaaccgac ttcctactaa ttttcaagaa gtcaggataa aaaggtctcc
     7681 cagtccaacc gctcgtttct catttctggc tcacgggcta aaatagtcca caggactatt
     7741 ttacttttcc gtaaactcgc cgtctacaac atcatcgcct gcgttgttag ctgtttgggc
     7801 accttcttgg cctgcggctg cttgttgtgc tgctgcggct tgctcgtaga gtttcactgc
     7861 aagggcttgg gctttttcgt tgaggttttc aagtttagcc ttcatgtcgt ccaagttgtt
     7921 agcttcttgg gctgctttca actcatcaag agcagcttgg gcttggtcac gttctgcgtc
     7981 gaaacctttt ccttcagttt ctttaagagt tttctcagtc gcaaagatgg cttggtcaac
     8041 atcgttacga aggtccactt cttccttacg tttcttatct gcttcagcgt ttgcttctgc
     8101 atctttcatc atgcggtcga tttcttcatc tgtcaaacct gagtttgatt ggataacgat
     8161 agtttgctct ttttgcgtac caaggtcttt agcttttaca gaaacgatac cgttcttgtc
     8221 gatgtcgaat gttacttcga tttgtggaat accacgaggt gctgcaggga tgtcagtcaa
     8281 ttggaagcga ccaagagtct tgttgtctgc ggccattgga cgctcacctt gaagcacgtg
     8341 gatatcaaca gctggctggt tgtctgccgc agttgagaag acttgtgatt tagaagttgg
     8401 gatagttgtg ttacggtcaa tgagttttgt aaagacgcca cccattgttt cgataccaag
     8461 tgacaatgga gttacgtcaa ggaggacaac gtctttgaca tcaccagtga tgacaccacc
     8521 ttggattgct gcacccatgg caaccacttc gtcagggtta acagatttgt ttggctcttt
     8581 accagtttca gccttaacag cttccacaac ggctgggata cgagttgaac caccgacaag
     8641 gataacttcg tcgatttctg acaagctaag tcctgcatct gaaagagctt gacgaacagg
     8701 tacttttgta cgttctacaa ggtcataagt caattcgtca aattttgcac gagtcaatgt
     8761 catttccaag tgaagcgggc ctgccgcacc ggcagtgata aacggcaagc tgatttgagt
     8821 tgatgtcacg cctgacaagt ctttcttagc tttttcagcc gcatctttca aacgttgaag
     8881 ggccatcttg tcaactgaca agtcgatacc gttttctttc ttgaattctg ctaccatgtg
     8941 gtcgataatc ttttggtcaa agtcgtcacc accgagcttg ttgtcacctg ctgttgcaag
     9001 tacgtcaaag acaccgtcac caagttcaag gatagatacg tcgaaagtac caccaccaag
     9061 gtcgaatacc aagattttct cgtctttgtc agtcttgtcc aaaccgtaag caagagctgc
     9121 tgcagttggt tcattgacga tacgttctac ttcaagacca gcgattttac cagcgtcttt
     9181 ggttgcttga cgttgtgcat cgttgaagta agcaggaaca gtaataacgg ccttggttac
     9241 tttttcacca aggtattctt cagcgtagcc tttcaaatat tgaagaatca ttgctgagat
     9301 ttcttgtggt gtgtattctt tgccatttgc tgaaactttt tcagaagttc ccatttttga
     9361 tttgatagag atgatagtat ctgggttagt tactgcttgg cgttttgccg catcaccaac
     9421 gatgatttca ccatttttga aagacacaac agacggagtt gtgcggttcc cttctgggtt
     9481 tgcgataatt tttgattcag ttccttcaag aactgcaact gctgagttag ttgtacctaa
     9541 gtcaataccg ataattttag acatgtgtgt tacctctttt taatttactt tcttttcaag
     9601 tagtttaacg acatctcggt caggattctt tttttgggga atagcagagc ggctactctg
     9661 atacgactac catagccggt ctcagcaagc gttcatgcag cttgtagcct ttttggaaga
     9721 cttgtgcaat gtgttctgct gggcagtcgt ctgtggctgg aactgtttga atggccatgt
     9781 gaagatttgg gtcaaagaca tcggttgcga cttcctccac cccttcttct ttgagggctt
     9841 gaatcaagct ttcctgtacc atttccaagc cttttttgac atcctctgtc aagccttcga
     9901 cttgaagggc acgttccaag ttatccaaac ttggcaagat tttcttggcc aagtcttgcg
     9961 aacggtagcg ttgaatagtt tggcgttcct cgttggcacg gcgttggata ttttgcattt
    10021 cagcgtgggc acgaaggtac ttgttttcaa attcctccgc acgctcattt gccaaatcca
    10081 attccgattt ttcaggtgtc tccacaactt cttctgttgc ttcaacctct tctacgattt
    10141 cttcgttttt gatttcttct gacaagttgt tcacctcttc attttctatt attactttga
    10201 acaataataa attgagttcg ggctagaaac tcagaaaagt gaaatagtcc agtggactgt
    10261 ttcagcctat ttttcaatcg tttccttcac gccctcacat cttaatttac ctcataatga
    10321 ttactgctca aataacggta gtaatcggtt aatttcataa ataggacgcg gctgatgata
    10381 ttgatcaaac tcatctgcct gcggtagttc atgtcaactg gaccgagcaa actcatctgg
    10441 cccatgcccc gataggggat tggaaaacga tgatgaatga ccgtcacatc tgcaagggcc
    10501 gagtcgctgt gttctgccac ggaaatgctg gtctgctggt cgggagccaa gccctgccgc
    10561 aactctggtg ctaatagctg tgcgctatcc aagagttggt aggtagctag attgccgtaa
    10621 gtcagcgagg caacttttcc actgataaag accgattctt gaaaaagatt ggaaaagatg
    10681 tagtccatca ggtccaagac attgtccgtc gtggcaaagt aacgctgcac cacttgggga
    10741 atctccgtcc gcagcttata gtggatggat aagaccgtct ggtcaacgaa gcgttcatcc
    10801 accaggcgtt tgagcacttc caagtccctg gtcaaaaagt ttttaggaat ggcaaactgg
    10861 acggtgactg gtttggactg gtccagagtc aagactgcca gagcatcgtg gctactgagc
    10921 tgcacgatgt caaaggaagt caactgctga ctggtcggct ccacatctaa gatgaccgaa
    10981 gtgtagccag tcaaatccgc taggactgta ctggcccgct ccaagatgtc ctccagcttg
    11041 aaggcttcaa agtcgaaggc cttgaccacc tgataaacat cttcttcatc gatgtgttcc
    11101 agattgagcg agtggttgac aaagtattga aaaccagccc gactgggcat ccgtccactt
    11161 gatgtgtggg ccttttccag caaacccaac tgctctagct tggccatgtc attgcgaatg
    11221 gtagccgaac ttgaagcaat catttcctgc aaggccttag aaccaactgg ctcatgatgg
    11281 cgcgtaaata attcaacaat cagattcaaa atatcatttt gacgttgggt aatcatctcc
    11341 gctcctttca tgaacacgat tgatttttag ctttagcact ctcgtatgac gagtgctaaa
    11401 ctatatactc ccattatacg ccgaaaaaat ggattgtcaa gagaaaaaga caaaaaatta
    11461 gcactctttt acctagagtg ctaaaaatca gtctgaggac ctagttactt ccctacttca
    11521 atcaaaaaaa tcagagaata tccctgattt catatcttta tttcccttgg gcaatcaatt
    11581 ctgctccacg ttttcggtag gataggtcgc caactgcttc aattaaaagc ttgccatttt
    11641 cgtatttgag gacggtaatt gagccgttat cgagaaagat attagcacca cgctctggct
    11701 ctaacagatg agccagggtg gcaatggtca tgccgtgact aaccacaaga gcattgccac
    11761 cgccctgttt tttcaaatct tgggcaatag cctcaaaacc tgtcaagata cggttactca
    11821 agacctccca cgattctgcc cagccagcgg tgtctgcttc ttgaatacct gctgcgattt
    11881 ccgcaaaagt catgcctgtc gcatcaactg tccctttcaa acgaggcagg acaccttgga
    11941 aaagctcggc atcgtacatg ccttcaaaac taccgaaaca ccattcacgg atacgcttgt
    12001 cttggtagta agggattttt cccaaaatcc ccaactcacg ctgggcgatg gtcatggtct
    12061 gaacggttcg acccaggtca ctggacacgg ctagtttaaa ttcaaggccc gcatccttga
    12121 gccccaagcc caattcacga atcccttcct caccagcctt ggtcagcggc gtatcacacc
    12181 agccctgcac gcgcccaatg gtattaaaca tggttttccc gtgacgagaa atatataatc
    12241 ttacatctgc cattcgtttt tctccttgtc atttcttact attagtataa caaaaaaccg
    12301 actgcttgtg gcaatcggtc tacgtttttt agttcttaaa gctgtgaatc ggtgctggaa
    12361 tttgtccgcc acggttgata aagtccgcag aagaagcctg attgaccttc atgactggcg
    12421 ctgttcccag aaggccgcca aattcgatca tgtccccttc ttttccaagc ggaataatgc
    12481 ggaccgcagt cgttttctgg ttgataaccc caatggccgc ttcatccgca atcatagctg
    12541 caatggtttc agcaggtgtt gtttctggaa tagcaatcat gtccaaacca acagaacaga
    12601 tagccgtcat agcttctaat ttttcaaggt taagggagcc attttgcacc gcagctatca
    12661 tgccctcgtc ctcagacaca gggatgaagg cacctgacaa gccaccgacc tggttacaag
    12721 ccatgacccc accctttttc actgcgtcat tgagcagagc aagggcagca gtcgttccgt
    12781 gcgtaccaac ggtttctaag cccatttcct ccaaaactcg tgccactgag tcgccaaccg
    12841 ctggcgttgg agcaagggac aggtccacga taccgaactt gacacccagg cgttcgctgg
    12901 ccatatttcc taccaactga ccgatacggg taatcttgaa ggcggtcttc ttgacggtct
    12961 ccgccaccac atcaaagctc tcgccacgca ctttttcaag ggcacgcttg accacaccag
    13021 gtccagacac gccgacatta atgaccacat ccgcctcacc gacaccatgg aaggcacccg
    13081 ccataaaggg attgtcctcg accgcattgg caaagaccac caacttggca gcccccatat
    13141 cagaagcctc cgccgtctcc ttgatgatcc gccccatgtc ccgcacagcc gtcatattga
    13201 tacccgtctt agtcgagccg atgttgaccg acgagcagac ttttgtcgtt tgggccaatg
    13261 cctgcggaat agagttgatg aggatttcat ctcctttttg gtagcccttt tgaacgagag
    13321 ctgaaaagcc cccgataaaa tcaattccga tctcatgagc cgccttgtcc aaggcatgag
    13381 ctaggggaag gtaatccgtg gcatccgtcg ccgcaccaat caaggcaata ggcgtcaccg
    13441 acacccgttt gttaacaaca ggaatcccca gctctgccgc aatctcatcg ccgactgcta
    13501 ccaagttctt ggccttggtg acaatcttgg tatacacttt ctccgcagct ttttcaatat
    13561 ccgcatcaat acagtccaag agggaaattc ccatggtgat ggtccgaata tcgaaattct
    13621 gctcctcaat catttcaatg gtttctctaa cctgtctaat atccatgaaa tctctcctta
    13681 caagttgtgc atagcatcaa aaatggctgc actttgaatg ttgattttca cgttcaaacc
    13741 ttgaccaaag gcttctagtt ctgcacgaag cttggtgaaa tctttcttct catccgacga
    13801 caccaccgcc atcatggtaa aatactcatc caaaacagtt tgggaaatat cgtcaatatt
    13861 gagccctaac tccgcaatct tagtcgcaac acccgcaaca attcctgact tgtccttacc
    13921 gacaactgta acaatagctt tcatctgaac acctcttcta aattttcatt tattttatca
    13981 taaatagaca gaaaaaggta aaaatctttt gatttctacc tgtatcgtac gtgtttacaa
    14041 ctccaactgt ttcttctatt cggctacggc tagagttgcc tagtttttaa gttgcaaact
    14101 ttctatttct accagtctgt gatacactag tgtcaccgaa ttctattatt tgttggttgc
    14161 gacaagctac caacctttct tttgttcaga aaggatactt atgaagatta tcaagcaatt
    14221 gtggtggttc ttttcccttg agaaaaaggc ctatctgatt gggattttat cactctgttt
    14281 ggtcagcctc ctcaatctcc tgcccgcctc tatcatgggg cagttgattg accagattgc
    14341 cagcggacag ttgactgagc atcgcttgct actcggggta gctgggctca tcttgtcagc
    14401 cttggccatg tacggcctcc gctatctttg gcgaatgaac atcctggcaa catcttatcg
    14461 tctaggaaaa atcatgaggg ctcggctgtt taaccacttt atgcacctat ccccttcctt
    14521 ttttcagcaa tatcgaacag gggatttaat ggcccatgcg accaatgata tcaatgcctt
    14581 gacacgacta gcaggtggtg gcgtcatgtc ctttgttgat gctaccgtta ctgccttggt
    14641 tacactggtc accatgagtg tgagcatttc ctggcagatg accttggttg caatcctccc
    14701 cctgcccttc atgacactca ctactaattt tctaggccgt cgtacccatg aaaatttcaa
    14761 ggcttcacaa gcagcctttt cagagcttaa caacaaggta caggaagctg tttctggtat
    14821 aaaggtcact aaatcctttg gctaccaaga acaagaaacg gcggcttttc aggaagtcaa
    14881 ccaagcagcc ttcttgcaga atatcaaaac catgcgttac gatgccctgt tcaaccctgc
    14941 cgtcctcttt tttattggcc tgtcctacct gctgaccctt ctggtcggct ctcacttcat
    15001 ctcgcaaggt caggtcagtc tgggccagct agtaactttc atgacctatt tagatctcct
    15061 cgtctggccg ctcatggcca tcggtttttt ggtcaatatt agccagcgtg gtgatgtatc
    15121 ttacaatcgg attcagaccc ttctcagcat ttcctcagag gtcattgaaa cagaccaacc
    15181 gctgcctgca ccaagcaatg gagagattgt ctatgagatt agcgaatttg cttatgataa
    15241 cataccagtc ctccaagaca tccagtttca catcaaaaaa gggcagacaa ttggtctggt
    15301 ggggccaaca ggctctggga agaccagcct actcaagctc ctcatgcgag aatacgatgt
    15361 gacaaatgga cagattttgc tcaatcagca aaacataaag cactacaagc tcggtgacct
    15421 acggcagttg atgggctatg ttccccaaga ccagttcctc tttgcaacca gcgtggctga
    15481 gaatatccgc tttggcaatc cagacctctc actagaccag gtgaaagagg ctgccaaagc
    15541 tgtccatgtc tatgaggata tccaggatat gccagatcgc tttgacacca tggttggtga
    15601 aaaggggatt tccctgtctg gtggacaaaa gcaacggttg gctatggctc gtgctatgat
    15661 tctcaatcca gatattctac tcttggacga ttcgctctcg gcagtggatg ccaagacaga
    15721 gcacgctatt cttgaaacca tcaagcaaga acgccttgat aagaccacca ttatcaccgc
    15781 ccatcgtctg tcagctattg tccatgccga tttgattctg gtccttgaag atgggaaaat
    15841 cgtagaacga ggtcgtcacc aagaattact agatgaaaaa ggctggtact acgataccta
    15901 tatgatgcag caactcgaaa aggaggaaag tgatgctctc taaaaccaag caagccagtg
    15961 ttttttggcg actcttgtcc tatctcaaag cctatcgact gctgacagtc cttgccttga
    16021 tctttttatt gctgactacc gtagtccgca gtctaattcc ccttctagcc tcttacttta
    16081 tagaccacta tatttcctcg atttctgaga cagctgcggc tattctagcg gcctactttg
    16141 ccctctatct gctccaaatg gtgcttcaat attttggaaa tctctggttt gcaaaagttt
    16201 cttacagcat tgtcagagat attcgccggg atgcttttgg gagaatggaa aagctgggaa
    16261 tggcttactt tgaccagact cctagtggct ccattgtctc gcgaatcacc aatgatacag
    16321 agagcatttc agagatgttt tcagggattt tatccagttt tatctcagct ctctttatta
    16381 tcatcacgac tctttcgacc atgtttgctc tggattggaa attgacctgt ctgatcgtcc
    16441 tctttcttcc cttgattttc ttattggttg atcaatatcg gaagcggtca gctcccattg
    16501 tggccaagac tagaagtctg ttgagtgcca tcaacagtaa actggcagag agcatcgagg
    16561 ggattcggat tatccaggcc ttttctcaag aggagaggct aaaggaagag tttgaggcta
    16621 tcaaccagga acatctggac tatgccagtc gttctatggc cttggacagt ctctttctcc
    16681 gacctgccct gtcacttgtt aagattttgg cctatgccct actcatgacc tattttggtt
    16741 tggactggtc acaggctgga atttctgctg gtcttatcta tgctttcatc cagtatgtca
    16801 atcgtctttt cgatcccttg ctggatgtca cgcagcattt ttccaccctt caaacctcta
    16861 tggtatctgc cggacgggtc tttacgctca tggaccagac ctttgaggaa ccgctacagc
    16921 tggaccgagg ggccaagatt gacaaggggc acattgtctt tgaggatgtt tgcttttcct
    16981 atgacggcaa gcggcaaatt ctggacaagg tttcctttga agtgagacag gggcagacca
    17041 tcgcctttgt aggtgctaca gggtcaggca agtcttctat catcaatgtc tttatgcgtt
    17101 tctatgagtt ccaatctgga cggattttga ttgacggtca ggatattcgt agcttttcgc
    17161 aggaagaatt gcggtccgcg atcggcctag tcctgcaaga tccctttcta tttcatggga
    17221 ccattgcatc caatatccag atgtatcagg atatttcccg agaggagatt gaggaagcgg
    17281 ctcgatttgt ggatgctgct ccctttattg agaaacttcc tgagaaatat gaccacctgg
    17341 ttactgagcg gggtaccgcc ttttcgacag gtcaacggca acttctggcc tttgcacgca
    17401 ccatggccag tcagcctaag attcttattt tagacgaggc taccgccaat atcgattcgg
    17461 agacagagca aacggtccag acttctcttg agaaaatgcg aaaagggcgg acaaccattg
    17521 ccatcgccca ccgcctatcg accattcagg atgccgactg tatttatgtc ttggataagg
    17581 gaaagatcat cgagtcaggc aatcacgaag aacttctagc cctcaagggt cactaccacc
    17641 gtatgtacca gttacaagca ggccagttaa cagcaaccta gaaaccactc catgacaaaa
    17701 gccaagttcc aaaatggacc tggctttttt atttttctac attgttgaga actgaagaca
    17761 tcaatctatc tctctttaac tcacattcgc tgaccgacgg gcataggctt tccagtcaat
    17821 cccacgcgct tcacaccagt tttgtatatt tggagccaag accaaatctt tctgccggag
    17881 ttccgcaata ttcttagcac ccagcatggt catgatttca gccatctgcc attgataagt
    17941 cttaatagcc tgcaagccgt cgtcaaaacg atgcccctta ccatccttga catactgaag
    18001 gaaatggttg gacataccaa caaggtctgc accgagggcc aatgatttga caatatctag
    18061 tggcgtctta ataccaccag atgcaatcag gcttgggcga acttcttcag aaacagacat
    18121 ggcttctacc aaggaagtca cggtagactg cccccaacct tctagataag tataatcatt
    18181 gaaggtacgg cgggcatttt caatcttggc gaaatccgtc cccccagtac cagacacatc
    18241 aatagtttgc acaccgacag aagccaactg ggcgacagtt tcacggctca tgccaaaacc
    18301 aacttccttg acgataacag gtacttccat ctcacgtaca agggtttcaa tatttttcaa
    18361 ccacatagta aagtcacggt ctccctcagg catgacaatc tcctgggggg cattgacatg
    18421 gatctgaatg gcatttgcct ccaacaaatc caccgcccgc ttggcattct caacactgtg
    18481 atgggcacct aggttggcaa agataatccc gtaaggattt tcccgacgca taacagagaa
    18541 ggtttccgca acagatggat ccttgatcgc cgcactgacc gaaccagaag ccagagcaat
    18601 cttcccaaaa tgtcccataa ttcccaacag acggttgatt tcccgcgtct tcttgcttcc
    18661 accggtcatg gcattgatga agaaaggaaa ggcaaagtcc aaaccagcta cgctggtcga
    18721 aatatccact tcatccactt tggtttgagg cagggaatga tggacaaaca aggtctccgt
    18781 aaagtccttg gccggtgtgg cactatattg ctggttggcc aagccaacgt gttgatcctt
    18841 gcggtctaaa ccaaattctc ctagataaaa catagggttc tccttattct ctacttgcga
    18901 tgtttagggt gagtggaaaa ataccggctt cttgccaagc cgtctctatc gctgcttttt
    18961 gttccttact gtctaccaag caaatgccgc agtcaccacc acctgcacca gatgacttgg
    19021 ccaccgcccc ataggtctgg gccaggtcac agagttgact cagttgaggc gtttcaatga
    19081 ccaaacccat tcctctcgca aagtcttgta agagcttgcg gttctgagta agggcctgcc
    19141 ttactgacgc tgagtcattt gtctgacaag ctactatcaa ctgctctaca cagatcttag
    19201 agtcggctag aaactggaca tgaatttgct ctttttctat ctgacttttt tggctttcca
    19261 tctgggaaac tagactatcc gttgaagccg ccgaccctgt ccagccaacc aagaggtcta
    19321 agccttgggg taactggata gggctgatag acaaactttg ccaatcactt tccaccagat
    19381 ccagcaggga cagctctgcc attttcccta aaagccaaga acggtccaga gaacgatagg
    19441 ctatcaagcc accaaaactg gaagccgcca aatctccgaa ggaacctgtc atgcctagct
    19501 tggtctgggc caaggctgcc agcttgtagg tcaggagggc atctggtcgg tgaccgtagt
    19561 aggtcaacag ggccttgacc gtcgcaaccg ttactgcccc tgacgacccc agaccatact
    19621 tggcacctga agtctggtca tccaggtcag actggaccgc tagactgtaa gttccatgac
    19681 aagcatagcc cttggctgtc agataggctt ctgtcacctg catgactgtc tcaatcagag
    19741 catagggatg gtcaccttgg acatgagcga taccttcctt acgctcccaa atcagataga
    19801 ggtctacttg ctggctggaa tgaagaaggc ctcggtcact tgtttcaatg gtgacggtca
    19861 ggtactggtc aaccgctgca atcaccgcag gataaccggc ctcgacgact gcgtattccc
    19921 ctgctagaaa gagttttcca ggtatcttta cttgtacttt catgctacaa gcccttttca
    19981 aacgctgctt gtgaggtctg ccactcgtct tcactgagga cgtaggcagc aggaccaggc
    20041 ttgctggtaa tgattttctc tcttgggaag actttggcca attcagctac tagctcatcc
    20101 atttgacttg ttcggcagag gaccttgaca tttggtcccg catccatggt catgtaggct
    20161 gataagccag ttgcttcacg aacctgacgg actgcctctt gagccactag gctatcggct
    20221 gaccaatagg taaagggtgg attggcagag agcgttgttg catgcatttt catgccattg
    20281 tgctctgtaa tctgaccaag tttttcaaaa tcacggctgg caatagctgc cttgatgtcc
    20341 accaagtctt gcttagcggt ttctacccag gctgtgtaaa atggtgaggt ggcaactgtg
    20401 tggtccatgc cctctcgact agcaatcttt ttcggtccgg tattgaccgc caagacgacc
    20461 atgccgatat cccaatccgc atcgtcaatg gggtgagcca tagaatcttc cgaaccagtt
    20521 cccatatccc actcaacaaa tccaccgaat aggctacggg tactagagcc tgagccccta
    20581 cgagcaaggg ttgatagggt ggctggtgac aaatctaaat ccaaagcagt tgcggttgca
    20641 agtgcaagtg ctgcgaaagc tgatgctgag cttgccaaac cagccgcagt cggaacaaaa
    20701 tttagacttt ccactcgggc aaatgccctt tcacctatat attcgcaaaa caaatccaaa
    20761 aatcgagaaa tttttaaaat ttctttttct ttttgtaaca ttccgttgag atagaactca
    20821 tcggcagtta attctgggtc aaaaacgacc ttggtatccg tataaaatgc gtcaagggtc
    20881 aaggataggc tagaattcat aggtagaaat aattccttat ctcgttttcc ccagtattta
    20941 atcaaggcaa tattggtatg ggcacgggct atgcctgttt gtttagtcat tggtttctcc
    21001 taagtactgt atccaggttt ggcgggcgcc tgcttggtca aggatgtgtg caagtttttg
    21061 ggcatcctga gctgttcttg ccagggcaat catgcagcct cctcggccgc caccagttaa
    21121 tttggctccg agggctccat tttcttgggc aaggctgacg agtttatcta ggctaggatc
    21181 agacacgcct aatttttgca ggagggcatg ggcttggttc attctgctgc ctagaagctc
    21241 tgcctggtct gtggctaaat cttccttggc ctgtcgggtc aagttaccca gttcttctac
    21301 tagcttgata gcctctggct ttttctccaa caaatcagcc acgtctgaaa tggcttctaa
    21361 ggtgtttccc gtcacacctg tgtcagccac taccaaatgg gcatggaatt tcagctcaaa
    21421 aggttcgatg ggctggtgct tgataaaaaa gacgggagac ttgccgctgg tggtcgctgc
    21481 atctatgcct gatggattgc cgtgggcaat tttttctgaa gactggacga tgtcccagag
    21541 ttcgctgtcg gtcagttctt ttttatagta ggcaaagagg gcgcgtgcta ctgcaactgc
    21601 cacggctgca ctggagccca taccgcgctc ggcaggaatg gtggaactaa tctcgatatg
    21661 gatagccggg tctgtcggtg ctccgatacg atagagcgag aaacggatgg catgcttgag
    21721 gctttcccag atttttggca tcttgtgcac caagccttta tagaagtcac aagcaacagt
    21781 cagcgcttcc ccttgagcag ttacctgagc aataatttca acagcagaaa aaggcatagc
    21841 aattgctggt tgcccataga ccaccgcatg ctctcccatc aaaatgattt ttccatttgc
    21901 ttttcctact accgacatac ttttctcctt atacaatcac ta
//
LOCUS       DBNFEG010000014        20385 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.014, whole genome shotgun sequence.
ACCESSION   DBNFEG010000014 DBNFEG010000000
VERSION     DBNFEG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20385
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.014"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(21..710)
                     /locus_tag="AC1PNG_000360"
     CDS             complement(21..710)
                     /locus_tag="AC1PNG_000360"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HIP6700897.1"
                     /translation="MKFGIIAAMPQELKILVEHLQDATEIDVLGRPYYQGRIGQHEVV
                     LVQSGIGKVMSAMSVAVLADRFSVDVIVNTGSAGAVAEGIAIGDVVVANQLAYHDVDV
                     TAFGYAYGQMAGQELYYPADQVLLEQLRTVLAEQEMISHVGLIVTGDSFIAGQERIAT
                     IKTHFPEVLAVEMEGAAIAQAAVNTGKPFLVIRAMSDTAQGDANITFDEFIIQAGERS
                     AQTLIAFLEKN"
     gene            complement(719..1039)
                     /gene="macP"
                     /locus_tag="AC1PNG_000361"
     CDS             complement(719..1039)
                     /gene="macP"
                     /locus_tag="AC1PNG_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HIP6700898.1"
                     /translation="MGKPLLTDEILEKVKKKQYSDQSSQFGHYRPNQDISMDDALFDP
                     YLEEDYQGYEEGQTIRIPVKPTVVKSRRIETIKREAFRSKVNKILFWVIILLIMFIIA
                     VLFI"
     gene            complement(1050..1595)
                     /locus_tag="AC1PNG_000362"
     CDS             complement(1050..1595)
                     /locus_tag="AC1PNG_000362"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIP6700899.1"
                     /translation="MNFEEKTIERTEIFKGHIFDVVVDDVALPNGGTGKRELIFHKGA
                     VCVLAVTPEGKMILVKQYRKAIERTIYEIPAGKLELGEEDTLEDAALRELEEETGYTS
                     DKLTLLADFYSAIGFCNERIRLYLADNLIKVENPRPMDEDEVIELHEVTLEEALTLVA
                     TGDICDAKTIMAIQYLQLMRK"
     gene            complement(1604..2986)
                     /gene="glmU"
                     /locus_tag="AC1PNG_000363"
     CDS             complement(1604..2986)
                     /gene="glmU"
                     /locus_tag="AC1PNG_000363"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIP6700900.1"
                     /translation="MSNNYAIILAAGKGTRMKSDLPKVLHKVAGITMLEHVKRAVDAM
                     EPAKTVTIVGHKAELVQAVLEGQSEFALQSEQLGTGHAVMMAEPALAGLEGQTLVIAG
                     DTPLITGESLKNLINFHVSHKNVATILTAQTDNPFGYGRIIRNADGEVQKIVEQKDAN
                     DFEKQVKEINTGTYLFDNKRLFEALKDINTDNAQGEYYLTDVISIFRQAGEKVGAYVL
                     RDFDESLGVNDRVALATAEAVMRKRINEKHMVNGVTFINPDATYIDIDVEIGAEAVIE
                     ANVVLKGQTVIGERTVLTNGTRVRDAKIAADVVISNSDIEESVIEEGVTVGPYAHIRP
                     GSLLKKDVHVGNFVEIKASTLGQGTKSGHLTYLGNATIGNNVNVGAGTITVNYDGKNK
                     FKTTVGDNAFVGSNSTIIAPVTIGDNALLAAGSVITKDIPEDAIGIGRCRQENKEGYA
                     TRFPFHPSQK"
     gene            complement(3163..3633)
                     /locus_tag="AC1PNG_000364"
     CDS             complement(3163..3633)
                     /locus_tag="AC1PNG_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HIP6700901.1"
                     /translation="MTPTQLWQEFLAINSQAGPEPESWAFGAEADRLADLVARGIKTS
                     TSSAHALYAVEGEEIPTAGGYDIILDGQGKAVCIIQTTKVYVTPFSQVTEKHAYKEGE
                     FRQGGDLSDIKEKSLIHWRQVHEELFTIWLAEAGLTFTDDMLVVCEEFELVYPK"
     gene            complement(3630..4181)
                     /locus_tag="AC1PNG_000365"
     CDS             complement(3630..4181)
                     /locus_tag="AC1PNG_000365"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6700902.1"
                     /translation="MILETNRLMLRPWSETDATDLFSQASHHEVGPAAGWPVHQSVEE
                     SREIIKTVLSQPETYALVLKETGQVIGSIGLMLGKQGGLDLPDSEAELGYWLGQGFWG
                     QGLVPEACQAILNYGFNQLNLEKVWCRAFVENSKSLRVQEKLGFVYQYLLEDVHFPLI
                     DEVRSERVNLLTREEWQSRKEKL"
     gene            complement(4194..4892)
                     /locus_tag="AC1PNG_000366"
     CDS             complement(4194..4892)
                     /locus_tag="AC1PNG_000366"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HIP6700903.1"
                     /translation="MTKTALITGVSSGIGLAQAEIFLENGWRVFGIDLASKPDLAGDF
                     HFLQLDLTGDLSPVFSWCQTVDVLCNTAGILDDYRPHLDIEEDELARIFAVNFFAVTR
                     LTRPYLQQMVDRQSGIIINMCSIASSLAGGGGSAYTASKHALAGFTKQLALDYAKDKV
                     QIFGIAPGAVQTGMTQKDFDPGGLADWVADQTPIGRWSQPSEIAELTLMLASGKLASM
                     QGQIITIDGGWSLK"
     gene            complement(4885..5121)
                     /locus_tag="AC1PNG_000367"
     CDS             complement(4885..5121)
                     /locus_tag="AC1PNG_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018367459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HIP6700904.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTIEVHGQRLEAT
                     PYFLINVTGEGEGFSMWSPTPCDVLATDWVEVHD"
     gene            complement(5174..5968)
                     /locus_tag="AC1PNG_000368"
     CDS             complement(5174..5968)
                     /locus_tag="AC1PNG_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700905.1"
                     /translation="MSKQMEYRKQIEVIESQLTKENKEYMGRINGYMMIASVFHRQEE
                     AVTAQLLSIYQDVLEAQKDGLSAEDFLGKDSKQMADDLLSYLPPIGFMEVANLSGLML
                     IIYLGSQWLMDFAGTGTISLNWLGLICDAVLSLLLPSGIFLIIRGLIYQTSKIKVWAS
                     FLYIPLLFLVICGLRLWAMPKEPDLVLTGWELLVPLTLLGLALLFFQKEKLVRYVFMP
                     SYLFMIAGGVVNMVMTVPVWLNLVLLILPAMAFWIGSAILLVRKPK"
     gene            complement(5970..6287)
                     /locus_tag="AC1PNG_000369"
     CDS             complement(5970..6287)
                     /locus_tag="AC1PNG_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIP6700906.1"
                     /translation="MKETQMLKGVLDGCVLQIISQKEIYGYELVQELRNQGFENMVGG
                     TVYPLLQKLEKNDLILSQNKPSPDGPDRKYFYLTDQGKAYLEDFWSQWTELVQKVQRL
                     KGE"
     gene            complement(6284..6448)
                     /locus_tag="AC1PNG_000370"
     CDS             complement(6284..6448)
                     /locus_tag="AC1PNG_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700907.1"
                     /translation="MKLDKKSRGNWKLAGITLVMAIITGWALDSILILGILLASGLMF
                     GFFTDDEEGL"
     gene            complement(6639..8651)
                     /locus_tag="AC1PNG_000371"
     CDS             complement(6639..8651)
                     /locus_tag="AC1PNG_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6700908.1"
                     /translation="MFGLTVRLALTNLKKNRRLYFPYALMTILSTAIAYIFASLAFHP
                     DLGQVKGADGVTMVLGFGMIVVMLAVAIMVFYANSFVMKQRSKEFGVYSILGLEKKHL
                     LLMTFLENLVFAASSILTGLLLGLALDKLFYALLLKVMQMPVVLASTFQLKSLVTVVI
                     YLFGIFALVSLFNIGKLGLTDSLKLVQGKKRGDKKTGFLWLQTLLALILLGAGYAIAQ
                     LVTSPMQAIPSFFGATLLVILGTYLLFHAGVISLLNWLKNRQTYYYKPDNFISVSNLI
                     FRMRKNALGLATITILSSMFLVTMVGGLNIYIGGKDYIANQNPNDYSIDVGMQPTTSK
                     AQMEKWEEWAEAILEETDIPVESKVVYPYQQAYFSEVTNQQVRFLTDEEAAGMNFSDR
                     VGIGFVLDQASYEKMTGEKLQLASDQVAIFSKGVQYQAGQTLTFDEKEMEVVQVLPKN
                     VTLGHLPDHGSFLLSQYLVIVVQDLAVFENQAENRYYMGFESSLSEEEQVDSHDTFLS
                     GSFESELWESNILPNGTNAISLAYRAYAMRGFFSFAGSLFFIGLLLSLIFLMAVVLVI
                     YFKQISEGYEDRDRFVIMTKVGLDEDQTRQSIRKQLLTVFFLPILLAFVHLAFAYKML
                     SSILLSIGVVNAGLMLQVTVGICGGYLLLYLVVYAITTRSYKQIIS"
     gene            complement(8653..9411)
                     /locus_tag="AC1PNG_000372"
     CDS             complement(8653..9411)
                     /locus_tag="AC1PNG_000372"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6700909.1"
                     /translation="MPLLDVQHVQKIYSSRFKAGQVEALKDIHFTVEKGEYVAIMGES
                     GSGKSTLLNILATLDKPTQGKVLLNGLDTQSIKSKEAAVFRREKLGFVFQDFNLLDTL
                     SVKDNVLLPLVLSRYPTDEMNKRLVHTLNSLGIATLQDKMPYEISGGQQQRVAVARAI
                     ITQPEILLADEPTGALDSKSTATLLDIFEKINQEGQTILMVTHSTVAASRAKRVLFIK
                     DGILYNQIYRGERTSQEMYQLISDTLTLMANRGE"
     gene            complement(9509..10501)
                     /locus_tag="AC1PNG_000373"
     CDS             complement(9509..10501)
                     /locus_tag="AC1PNG_000373"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIP6700910.1"
                     /translation="MNKDDFGSLVPGFLTSWLGHRLIFLIAYAVFAVAILAYSSLFDI
                     KRNLVFYAILLLSICWLLALLWDFFREFSRFGKIWRGQKVATGTASERLLLEKVERLE
                     VQNKQILEKQRQEQTELDDYYTLWAHQMKTPIAASQLLVKEVDSSPVRHQLESELFKI
                     EQYTGLVLNYLRLQSFHDDLVIEAVNLEELVRSLVKKYSLFFIQANTSLDLGQLDRLV
                     KTDKRWLGLLIEQILSNALKYCQEGTVSIFLDGDELVIRDTGIGIAESDLERVFERGF
                     SGFNGRRTQQSSGLGLYLSRKIAAELGYSLKLQSKVGQGTEVRIGIKEVELIFD"
     gene            complement(10494..11177)
                     /locus_tag="AC1PNG_000374"
     CDS             complement(10494..11177)
                     /locus_tag="AC1PNG_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIP6700911.1"
                     /translation="MQKILLIEDDKTISQLVAKNLINWGYQVQEVKDFQMVLEQMEEF
                     QPHLILLDIGLPFFNGYYWCQEIRKTSRVPIMFLSSHDQPMDIVMAINMGADDYVTKP
                     FEMTVLLAKIQGLLRRTYDFVGEQSLLWFEEISLDLKTMQVSYGQVVEELTRNEFQIL
                     RVLFEHGKEVVSREELMRELWNSDIFVDDNTLSVNIARLRKKLAELGLPDVIATKKGV
                     GYGLVWTHE"
     gene            11347..12516
                     /locus_tag="AC1PNG_000375"
     CDS             11347..12516
                     /locus_tag="AC1PNG_000375"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HIP6700912.1"
                     /translation="MPNLLTYIEETQYDNFYDKPINKLDILALTELSYLPFDNLVPYS
                     FTENGVRLDRLAATFEETYKNNFPPFSMVTKNRLALLGLLAKSIRFKSIKAFGFVDDY
                     QLEQEKQFSAISYRIDRKTIVTCFRGTDDTIIGWKEDFHMTYMDEIPAQRAASGYLEK
                     LMMQQGGHFYLAGHSKGGNLALYAACQQATDQQDRILAIYPFDSPGLHKKHLEAPSYQ
                     AIHQRIFPVIPQNSIVGMMLETPENVQIVQSNTLGILQHISFSWEVDGSDFKPAPALT
                     SDSLQTDQALKTWTASLTDDELRDFFDLFFGIFIKAGIERFSDITVNPLQKLQEIDRL
                     RKEIAPEEAEMVDKLIRLLFDTRYQIWRDNIPSPEISLPDWRKLFQRNTTENKEN"
     gene            complement(12531..13655)
                     /locus_tag="AC1PNG_000376"
     CDS             complement(12531..13655)
                     /locus_tag="AC1PNG_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HIP6700913.1"
                     /translation="MKLPRFGVEEWLNVHETSATYDIAGVSISALTLEELFALSGTNP
                     EDFYKKLQGTKLNYGWIEGSPAFKEAVSQLYEQVSPEQILQTNGATGANLLVLYSLIE
                     PGDHVISLYPTYQQLYDIPKSLGAEVDLWQIEEEHGWLPDLEKLRQLIRPNTKMICIN
                     NANNPTGAVMDRVYLEELAEIASEVGAYILSDEVYRSFSGLDVPSIIEVYDKGIAVNS
                     LSKTYSLPGIRIGWVAANHQVTDILRDYRDYTMICAGVFDDLVAQLALAHYKEILERN
                     RHILEENLAILDQWIEEEPLVSYIRPAVVSTSFVKIAVDMPMEDFCLQLLQEHGVLLV
                     PGNRFERDGYVRLGFACEQETLIKGLEKLSQFLRRFDKEN"
     gene            complement(13805..14566)
                     /locus_tag="AC1PNG_000377"
     CDS             complement(13805..14566)
                     /locus_tag="AC1PNG_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIP6700914.1"
                     /translation="MTNQLENAKNLYLRGIRDGEIKEVHENYMGVSYTQHSTGVPDEK
                     EGFATFFEDFFKRNPKREITIVRAIEDGNFVFVHVHKKLNDGEAEWVTADIFRADENG
                     RIVEHWDVIDAYPKTIGQTDPIYADFELTDLDKTEDNKKIVRRFLVDVLQNGEIEKFN
                     DYVAADLIQHNQEIAQGGAAYKNYLVDKAVNYDFVFKVMGQGDYVVAYSKVWIAGQDY
                     AHFDIYRLKDGKIVERWDNKEVMPDKKDLTNLGKF"
     gene            complement(14576..16090)
                     /locus_tag="AC1PNG_000378"
     CDS             complement(14576..16090)
                     /locus_tag="AC1PNG_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019319700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HIP6700915.1"
                     /translation="MDQLVTTFLERKADWLTALFEHLRISLLALIIAIAIAVPLGLVL
                     SNKKKLTEWSLQVTGVFQTIPSLALLGLFIPFMGIGTLPAVVALVIYAIFPILQGTLT
                     GLSEIDPSLEEAATAFGMTKMEKLRKFQLALAMPILMSGIRTASIMIIGTATLAALVG
                     AGGLGSFILLGIDRNDSALILIGAVSSAVLAVLFGSVIKVLQDKKPRTILAALLVTLL
                     AVGASYVPLNQSSSNKLVIAGKLGAEPEILINMYKLLIEDQTDIEVELEPNFGKTSFL
                     YEALKSGSIDIYPEYTGTITTTLLTNPPSDLSNNPEEVYTYAKEAILEQDGLMYLAPM
                     AFQNTYALAVTEDYAQDHGLEKISDLAKVQQTAVAGFSLEFNDREDGNVGLTNLYGLN
                     LNVKTMEPALRYEAIKNGDVQLVDAYSTDSKIVSYKLKILEDDKELFPPYQAAPLLTK
                     ETLDKYPELEQVLGDLAGNISTEEMTQMNYAVDVEGKSAEQVAREYLEKENLLK"
     gene            complement(16090..16821)
                     /locus_tag="AC1PNG_000379"
     CDS             complement(16090..16821)
                     /locus_tag="AC1PNG_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP6700916.1"
                     /translation="MIEYQNVSLTCQVNGPILKNLTFDIQEGEFFVLIGPSGSGKTTT
                     LKLINRLIEQTDGDIRIQGKRLKDFDLRKLRLETGYVLQQIALFPNMTVAENIALIPE
                     MKGLNKEETLKKSRQLLNKVGLEPDSYLDRLPKDLSGGEKQRIGILRAIIANPKVLLM
                     DEPFSALDPISKGQLQDLIKGLHEEFKMTTVFVTHDMDEAVKLADRICLMKVGEVVQL
                     GTPDELRNQPANDFVVEFMKNRGGA"
     gene            complement(16814..17263)
                     /locus_tag="AC1PNG_000380"
     CDS             complement(16814..17263)
                     /locus_tag="AC1PNG_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIP6700917.1"
                     /translation="MTELRQRSKILARIFYNQDGLYEDYARRHGLNSKSLSILMFIYY
                     ISRGVTQNWISKKTYSSKQVVNATIKKFLDKGYVFFEENPADKRHKKVKLTEEGRVYA
                     SRILDPLEEAEKTALSQFSLEEQEQLLDLFGRYGQALTEILGGETND"
     gene            complement(17572..18108)
                     /locus_tag="AC1PNG_000381"
     CDS             complement(17572..18108)
                     /locus_tag="AC1PNG_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIP6700918.1"
                     /translation="MVKKSSSVWTSLLLGAAGGAAAAAFLASKTGKTVKEKVVNFAND
                     YKENYEEINADFVTKAQDLGKQATERFTEVKTQLETGELTVEDLVKSGKEKSLETFEQ
                     IKEKIAEQNLSTADILEAIKAKTAKAPTVDLTEEDIEDAVVVSEDIEIDIEDVQTEVV
                     SETVSEVASETASETVEG"
     gene            complement(18092..18502)
                     /locus_tag="AC1PNG_000382"
     CDS             complement(18092..18502)
                     /locus_tag="AC1PNG_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HIP6700919.1"
                     /translation="MIEISVLIIALSIAAVAVYIVLLLKKLGTVTDEAQQTLKVLTSD
                     VNVTLYQTNELLAKTNVLVEDVNGKFSTLDPLFVAVADLSTSVSDLNASARDLTVKAK
                     SAGASTVKAGGALSALSTLSSLLGKKGEKNGKKI"
     gene            complement(18516..19292)
                     /gene="lgt"
                     /locus_tag="AC1PNG_000383"
     CDS             complement(18516..19292)
                     /gene="lgt"
                     /locus_tag="AC1PNG_000383"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIP6700920.1"
                     /translation="MDPIAIKLGPLEIRWYAICILLGLILGVYLATKEGPRKKIRQDD
                     ILDFILIAFPLSIIGARIYYVAFSWSEYKDNILSIFAIWNGGIAIYGGLITGAIVLYF
                     FTQYRFINTLDFLDIVAPSVMIAQAIGRWGNFFNQEAYGKAVESLNYLPAFIRDQMYI
                     DGAYRQPTFLFESLWNLLGFGLVCVLRRRPKFLKQGEITAFYLVWYGCGRLLIEGLRT
                     DSLMFLGIRVSQWLSGVLILVGIIMVVLRRRKSSIPFYQP"
     gene            complement(19294..20226)
                     /gene="hprK"
                     /locus_tag="AC1PNG_000384"
     CDS             complement(19294..20226)
                     /gene="hprK"
                     /locus_tag="AC1PNG_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIP6700921.1"
                     /translation="MTVYVKDLVDNLRIECAYSTEELLQKQITTADITRPGLEMTGYF
                     DYYAPERIQLMGMKEWSYLMAMTAHNRYQVLSQMFQPETPVVIVARGLEIPEEMYKAA
                     KEKQIAICRSKTATSRLSGELSSYLDSRLAQRTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDDVTLWGEPAEILRHLLEIRGVGIIDIMSLYGAS
                     AVKDSSEVQLAVYLENFETGKVFDRLGNSGDTIEIAGIAIPQIRIPVKTGRNISVVIE
                     AAAMNYRAKQMGFDATKIFEERLSNLIEHNREEA"
BASE COUNT         5743 a         4961 c         4027 g         5654 t
ORIGIN      
        1 gtattgttgt caaaatctgc ttaatttttc tccagaaagg caatcagggt ctgggcagaa
       61 cgttctcctg cttgaatgat aaattcatca aaggtgatat tggcatctcc ctgagctgtg
      121 tcactcatgg cacggataac caagaatggc ttacctgtat tgacagctgc ttgagcgata
      181 gctgctcctt ccatctctac ggcaaggact tctgggaaat gagttttgat cgtagcgatt
      241 ctctcctgac ctgcaataaa actgtcacct gtcacaatca gacccacatg gctaatcatt
      301 tcctgctctg ctaaaacggt tcttagctgt tctaacaaga cttggtcggc tggataatac
      361 aattcctgcc ctgccatttg cccgtaagca tagccgaaag ccgtcacatc cacatcgtgg
      421 taagccaatt gattcgctac aaccacatca ccgatggcaa taccttcggc aacagctcct
      481 gccgaacctg tattgacaat gacatcaacc gaaaaacgat ctgccaaaac agccacggac
      541 atagctgaca tcaccttacc gatacctgac tgaacaagaa caacttcatg ttgaccgatt
      601 cgtccctgat agtaaggtcg acccaagaca tcgatttcgg ttgcatcttg tagatgctcg
      661 accaaaatct ttaattcctg aggcatggct gcgataattc caaatttcat ctcttctcct
      721 aaataaataa tactgcaatg ataaacatta tcagtaagat gatgacccaa aaaaggattt
      781 tgtttacctt actgcggaaa gcttctcgct tgatggtttc gatacggcga ctttttacta
      841 cagtaggttt gaccgggatt cggatcgttt gtccctcttc atatccctga taatcttctt
      901 ctagatatgg atcgaataga gcatcatcca ttgatatatc ctggtttgga cgatagtgtc
      961 caaactggct agattggtcg gaatactgtt tctttttcac tttctctaaa atttcatccg
     1021 tcaacaaggg cttgcccatt gctagtttcc tactttctca tgagttgtaa atattggata
     1081 gccatgatgg tctttgcatc acaaatatcg ccagtagcca ccaaggtcaa agcctcttcc
     1141 aaggttacct catgtaattc gataacctca tcttcatcca ttggacgagg attttcaacc
     1201 ttaataaggt tatctgctaa ataaaggcga attcgttcat tacagaaacc gatggctgaa
     1261 tagaaatctg ccaacaaggt cagtttgtca ctagtatagc ctgtttcttc ttctaattca
     1321 cgcaaagcag catcttcaag cgtatcttct tcgcctagct ccaatttccc cgccggaatt
     1381 tcataaatag tacgttcaat agctttgcgg tattgtttga ccaaaatcat tttaccttct
     1441 ggggtaacgg ctaaaacgca aactgctccc ttgtggaaaa tgagttcgcg cttaccagtt
     1501 ccaccatttg gcaaggcaac atcatctact actacatcaa aaatgtgccc tttaaaaatt
     1561 tctgtccgct caatggtttt ttcttcaaaa ttcatatgat tacctatttt tgacttggat
     1621 ggaacggaaa acgagtcgca taaccttctt tgttttcttg acgacaacga ccaataccga
     1681 tggcatcctc tggaatgtct tttgtaatga cagaacctgc agccaacaag gcgttgtccc
     1741 caatagttac tggtgcgata atggttgagt tagagccaac aaaggcatta tctccgacgg
     1801 ttgttttaaa tttattttta ccgtcgtagt tgacagtaat ggtaccagca ccaacgttaa
     1861 cattattgcc aatcgttgca tttcctaggt aggtcaaatg accagacttc gttccttgac
     1921 caagtgtaga ggccttgatt tccacaaagt ttccaacgtg aacatctttt ttcaggagac
     1981 tgcctggacg aatatgggcg tatggtccta ccgtcacacc ttcttcaatg acagattctt
     2041 caatatctga gttgctgatg acgacatctg ctgcaatttt cgcatcacgt acacgagtac
     2101 cattggttaa aacagtacgt tcaccaatca ccgtttgccc tttcaagaca acattagctt
     2161 caataacagc ttctgcacca atctcaacat caatatcaat ataggtagca tctgggttga
     2221 taaatgtcac accattgacc atgtgttttt cattgattcg tttgcgcata acagcttctg
     2281 cagtcgcaag agcgacgcga tcattgacac ccaaactttc atcaaaatca cgcaatacat
     2341 aggcaccaac cttctcacct gcctgacgga aaatcgaaat aacatctgtc agatagtact
     2401 caccttgggc attgtccgta ttaatatctt taagcgcttc aaaaagacgt ttattatcaa
     2461 acaagtaggt tcctgtattg atttctttca cttgtttttc aaaatcgttg gcatcttttt
     2521 gctcaacaat tttttgcact tcaccatcag cattgcgaat aatccgacca tatccgaatg
     2581 ggttatcagt ctgagctgtc aagatagtcg caacattctt gtggctgaca tgaaagttaa
     2641 tgaggttctt caagctttct cctgtaatca gcggtgtatc tcctgcgata acaagggtct
     2701 gaccttccaa acctgcaaga gctggctcag ccatcataac ggcgtggcct gtccccaact
     2761 gttcagattg aagggcaaac tctgactgac cttcgagaac tgcttggacc aattctgcct
     2821 tgtgaccgac aatcgttact gttttagcag gttccatagc atctacagcc cgtttgacat
     2881 gctccaacat ggtaattcct gccactttgt gtaagacctt tggcaagtca gacttcatgc
     2941 gagttccctt acccgccgct aggataattg catagttatt gctcatagtt ttctctcttt
     3001 tcaatgatat actcctatta tagcaaattt tgatgttttt ggaaaatagc agtattatag
     3061 ggaattttcg taaataaaaa agaaactgga atgaagccag tttctcaaaa atttctagta
     3121 ttagaaaaca aactacaggt tgattgcatc caatagaatt gtctatttag gataaaccaa
     3181 ttcaaattct tcacagacaa ccagcatgtc atctgtgaag gtcagacctg cttcggccag
     3241 ccagatagtg aaaagctctt catggacctg tcgccagtgg attagacttt tctcttttat
     3301 atcagacaag tcacctccct gacgaaactc gccctcctta taggcatgct tttccgtcac
     3361 ttgggaaaag ggcgtcacat agaccttggt ggtctggata atgcaaacag ctttaccttg
     3421 tccgtccaga atgatgtcat agccaccagc tgttggaatt tcttctccct ctactgcgta
     3481 gagtgcatgg gcagagctgg tcgaggtttt gatgcctcta gccaccaaat cagccagtcg
     3541 atcagcttcc gccccaaagg cccaggactc cggctcagga ccagcttggg aattgatagc
     3601 aagaaattct tgccagagtt gtgtaggggt catagttttt ccttcctaga ctgccattcc
     3661 tctcttgtca gcagattcac tcgttcgcta cggacctcat caatgagtgg gaaatgcaca
     3721 tcttccaaga ggtattggta aacaaagccc agtttttctt ggacacgtaa tgatttgcta
     3781 ttttctacaa aagcacgaca ccagactttc tctaaattca actggttaaa gccatagttc
     3841 agtatagctt gacaagcttc tggtaccaga ccctgtcccc aaaatccctg tccaagccag
     3901 tagcctagct cagcttctga atcaggcaaa tcaagaccac cttgtttacc aagcatgagc
     3961 ccgatactgc caatcacctg tcctgtttct ttgagaacaa gggcgtaggt ttctggttgg
     4021 ctaaggactg tcttgataat ttctcgactt tcttccacgc tctgatgaac aggccaaccc
     4081 gctgcaggac caacttcatg atgactggcc tgagaaaata agtcagttgc gtctgtttca
     4141 ctccaaggtc ggagcatcaa tctattggtt tcaagaatca tcgaatttcc tccctacttc
     4201 aaactccagc caccgtcaat ggtgataatt tggccttgca tggaagccag tttgccactg
     4261 gctagcataa gggtcagctc tgcgatttca ctgggctggg accagcgtcc gataggtgtc
     4321 tggtctgcca cccaatccgc cagaccacca gggtcaaagt ccttctgggt catgcctgtt
     4381 tggacggcac ctggggcaat gccgaagatc tggaccttgt ccttggcata gtccagtgcc
     4441 aactgcttgg taaagcctgc caaagcgtgc ttggaggcgg tataggctga acctcctcca
     4501 cctgctagac tggaggcaat ggagcacatg ttgataatga tgccagactg tctgtccacc
     4561 atttgttgca gatagggacg ggtcagtctg gtcaccgcaa aaaaattgac cgcgaagatg
     4621 cgagctaatt catcctcctc aatatccagg tggggacggt agtcgtctag aatgcctgcg
     4681 gtgttacaga ggacatcaac tgtctggcac catgaaaaga cgggtgataa atcgcctgtc
     4741 aggtccagct gtagaaagtg gaaatcgcct gccaaatcag gcttgctagc cagatcaatc
     4801 ccgaagacac gccagccatt ttccaaaaaa atctccgcct gagctagtcc aatgccgctg
     4861 gaaacgccgg tgatgagggc tgtcttagtc atggacttcc acccagtcgg tcgcaaggac
     4921 atcgcagggg gtcgggctcc acatggagaa gccctcgccc tcgccggtca cgttgatgag
     4981 gaaatagggc gtcgcctcca gcctttgccc gtggacctcg atggtgtcaa agagctggac
     5041 gtagttctcc gccccgcccc agcctgtgcg aacgtacttt ttcttggcct tcaagcctgg
     5101 taaaatctct tcaaatgtca tggtttactc ctttattctt ctatctttca accattatac
     5161 caaaaaatcg cttctatttc ggttttctca ccaacaaaat cgcacttcca atccaaaagg
     5221 ccatagctgg cagaatgagc aaaaccagat tgagccaaac tggaacggtc atgaccatat
     5281 tcaccacacc gccagcaatc atgaacagat aactaggcat aaagacatag cggaccaact
     5341 tttccttttg gaaaaatagc agagcgagac cgagcaaggt gagtggtact agaagctccc
     5401 agccagtcaa gactaagtca ggttccttgg gcattgccca aagacgaagg ccacaaatta
     5461 cgagaaaaag aaggggaatg tagagaaaac ttgcccaaac cttgattttg ctggtctggt
     5521 aaatcagacc gcgaataatg aggaaaatcc ccgaggggag gaggaggcta agtaccgcgt
     5581 cgcagatcaa gcccagccag ttgagcgaaa tggtgcctgt acctgcaaaa tccatcagcc
     5641 attggctgcc gaggtagatg atcagcatga ggccactcag gtttgcgact tccatgaaac
     5701 caatcggagg gaggtagctg agtaggtcgt ccgccatctg cttgctgtct ttgcccagaa
     5761 aatcctccgc agaaagcccg tctttttgag cttcaagaac atcttggtaa atggataaga
     5821 gctgggcagt cactgcttct tcctgcctgt gaaaaacaga ggcaatcatc atataaccat
     5881 tgatacggcc catgtattcc ttgttttcct tggtcagctg agattcaatc acttcaatct
     5941 gcttgcggta ttccatttgt ttgctcatct tattctcctt tcaatcgttg aaccttttgg
     6001 accaattccg tccactggct ccaaaaatct tctagataag cttttccctg gtctgtcaga
     6061 tagaaatact tcctgtctgg tccgtctggc gagggcttat tctgactaag aatcaggtca
     6121 tttttttcta acttctgaag gagtggataa accgtccctc cgaccatgtt ttcaaagccc
     6181 tgatttctca actcctgaac caattcataa ccataaatct ccttctggct gataatttgt
     6241 aatacacagc catctaggac ccccttgagc atttgagttt ctttcataag ccctcctcat
     6301 catctgtaaa aaatccaaac atcaagcctg acgctaacag gatgcctaag atcaaaatag
     6361 aatccaaagc ccagccagtt ataatggcca tcacaagcgt gatgcctgct agtttccaat
     6421 ttccccgact tttcttatcc agtttcatac tgcctcccta cctagtttgt aatacagact
     6481 agtttgataa aaataaaagt gattttcttt gtcttctatc actagtttgt tttacctatt
     6541 agctttgtat ctatagtata acacaaggcg gctagtatgt aaaacaaaat agtgaaaaat
     6601 attcaaaaaa aaaataaaaa acttgggttg ccccaagttt aggaaataat ttgtttgtaa
     6661 gaccgtgtcg tgatggcata taccactagg taaaggagga gatagccacc acagatgcct
     6721 accgttactt gcagcataag ccctgcattg accacaccaa tagacaacaa gatagacgag
     6781 agcatcttgt aggcaaatgc aagatgaaca aaggcaagta ggataggtag gaaaaagacg
     6841 gtcagcaatt gcttgcgaat ggactgtctc gtctgatcct catccagacc aaccttggtc
     6901 ataatgacga agcgatctct gtcttcatag ccttctgaaa tctgtttgaa gtaaatcacc
     6961 agcaccaccg ccatcaaaaa gatgagtgat agcaagaggc cgatgaagaa aagtgaacct
     7021 gcaaaactga agaaaccacg catcgcatag gcacgatagg cgagactgat ggcgttcgtt
     7081 ccattcggta atatattgga ctcccaaagc tcactttcaa aacttcctga taaaaaggtg
     7141 tcatggctat ctacctgctc ttcttctgat agactgcttt caaatcccat ataatagcga
     7201 ttttcggctt gattttcaaa aacagccaag tcttgaacaa caatgaccag atactggctg
     7261 agaaggaagg aaccatggtc tggtagatgt cctaaagtca cattcttggg caagacttgg
     7321 acaacttcca tctccttttc atcaaaggtg agggtttgac cagcttgata ctggactcct
     7381 ttgctaaaaa ttgccacttg gtcagatgcc aactggagtt tttctcccgt catcttttca
     7441 tagctggctt gatcaaggac aaaaccgatc ccaactcggt cagagaaatt cataccagcg
     7501 gcttcttcat ccgtcaaaaa tcttacttgc tgatttgtca cctcagaaaa ataggcttgc
     7561 tgataaggat agaccacctt actttcaaca ggaatatctg tctcttctaa gatagcttcc
     7621 gcccactcct cccacttctc catttgagct tttgaagtgg tgggctgcat accaacatca
     7681 atcgaatagt catttggatt ttgattggca atataatctt tgccaccgat gtaaatattg
     7741 agtcctccaa ccatggtcac caagaacata ctggagagga tggtaatggt cgcaagccct
     7801 agagcatttt tccgcatgcg gaaaatcaag ttggatacag aaatgaaatt atctggctta
     7861 taataatagg tctgacggtt ctttagccag tttaagagac tgataacacc tgcatggaag
     7921 agcaggtagg tcccaaggat aaccagaaga gtcgctccga agaaacttgg aatagcctgc
     7981 atcggactgg tcaccagctg ggcaatggcg tagcctgccc ccaataagat aagagctaag
     8041 agggtttgta gccagaggaa gcccgttttc ttgtcccctc tcttcttacc ttgaaccagt
     8101 ttgagtgagt cggtcaagcc caacttccca atattaaaaa ggctgaccaa ggcaaaaatc
     8161 ccaaagagat agatgactac agttaccaga cttttcaact ggaaagttga agccaagacc
     8221 acaggcatct gcatgacctt gagcaagagg gcatagaaga gtttatctag agccaagccc
     8281 agcaacaatc ctgtcaaaat actactggct gcaaaaacta gattttctag aaaagtcatc
     8341 aggaggaggt gttttttctc caatcctaga atactataga caccaaactc ttttgaccgc
     8401 tgcttcatga caaaactatt ggcatagaag accatgatgg caaccgccaa catgaccaca
     8461 atcataccaa agcccaatac catggttacc ccatcggctc ccttgacctg acctaaatca
     8521 ggatgaaagg caagcgaggc aaaaatatag gcaatggctg tggacaaaat ggtcatcagg
     8581 gcatagggaa aatagagtct gcggttcttt ttgagattgg tcagagccaa tcgaacggtt
     8641 agtccaaaca tactactcac ctcgattcgc cataagtgtc aaggtatcgg aaatcaactg
     8701 atacatttcc tgactggttc tctcacctcg gtagatttga ttgtagagga tgccgtcctt
     8761 gatgaagaga acccgtttgg cacgactagc tgctactgtc gagtgggtta ccatcaaaat
     8821 ggtttgcccc tcttggttga ttttttcaaa aatatctaga agagttgccg ttgatttgga
     8881 gtcgagggcc ccagtcggct catccgctaa gagaatttct ggctgggtaa tgatggcacg
     8941 cgccaccgca actcgctgct gctgaccacc cgaaatctca tagggcatct tgtcttgcaa
     9001 ggtcgcaatt cccaggctat taagcgtatg aacgagtcgt ttgttcatct catcagtcgg
     9061 atagcgagat aaaaccagag gcaagagcac attatccttg actgagaggg tgtccaagag
     9121 attgaaatct tggaagacaa agcccaattt ctcccgacga aagactgccg cttccttgct
     9181 cttgatagac tgggtatcca atccgttaag caagaccttg ccctgcgttg gcttatcaag
     9241 ggttgccaaa atattgagca aggtggactt accagaaccc gactcaccca taatggcgac
     9301 atattcaccc ttttcaactg taaaatgaat atccttcaag gcctcaacct gacccgcctt
     9361 aaagcgagaa ctatatattt tttgaacatg ttgtacatct aagagtggca tcactgcaac
     9421 ctccttttct ttttctacct ccattctatc ccatccgcca cccctctgcc atagccctag
     9481 ctttcatttg cgtgacagtt ttgtaagatt agtcaaaaat caactccact tctttgatac
     9541 cgattcgcac ctctgttccc tgacccactt ttgattgtag ctttagggaa tagcccagct
     9601 ccgcagcaat tttccgtgag agatagagcc ccagacccga agattgctgg gttctgcggc
     9661 cattgaaacc tgagaaacca cgctcaaaga cccgttccaa gtcgctctca gcaatcccaa
     9721 ttcccgtatc gcgaatgacc aactcatccc catctagaaa aatcgaaaca gtcccttcct
     9781 gacaatactt gagggcattg gataaaattt gttctatcaa gagacccagc cagcgtttgt
     9841 cggtcttaac aagcctatcc aactgcccca agtccaagct agtattagcc tgaatgaaaa
     9901 atagggaata tttcttgacc agacttctga ccaactcttc caaattgacg gcttcgataa
     9961 ccaaatcatc atgaaaggac tgtagacgga gatagttcaa gaccaagcct gtgtattgtt
    10021 ctatcttgaa caattccgat tctaattgat gacgaactgg actactatct acttccttga
    10081 ccaaaagttg actggctgca atcggcgttt tcatctggtg ggcccagagg gtgtagtagt
    10141 catctaattc tgtctgttcc tgtcgctgtt tttctaggat ttgcttgttc tggacttcta
    10201 gtcgctctac cttctccagt aacaaacgct cactggctgt cccagtagcc actttttgac
    10261 cacgccagat ttttccaaag cgggaaaatt ccctgaaaaa atcccagagg agggcgagga
    10321 gccaacaaat actgaggagg aggatggcat agaaaaccaa gtttcttttg atgtcaaaga
    10381 ggctactata agccagaata gccacagcaa aaacggcgta ggctatcaga aaaatcaagc
    10441 gatgaccaag ccatgaggtc aaaaatcctg gtactaatga gccaaaatca tccttattca
    10501 tgtgtccaca ccaggccgta gcctactcct ttcttggtcg caatcacatc tggcagaccc
    10561 agttctgcca atttcttacg caggcgagcg atgttaaccg acagggtatt gtcatccaca
    10621 aaaatatcac tgttccagag ttctctcatc agctcctcac ggctgaccac ttccttgcca
    10681 tgttcaaata aaacgcgtaa aatctgaaat tcatttcgcg tcagctcttc cacaacctgt
    10741 ccataggaca cctgcatggt ctttaggtct agggaaatct cctcaaacca gagtaaactt
    10801 tgttctccga caaagtcata ggttcttctg agaagcccct gtattttagc caggagaacc
    10861 gtcatttcaa agggcttggt cacatagtca tccgccccca tattgatcgc cataacaata
    10921 tccattggct ggtcatggga agaaagaaac ataatgggca cacgggaggt cttgcgaatt
    10981 tcctgacacc agtaataacc attgaaaaac ggcaggccaa tatccagtaa aatcaaatgg
    11041 ggctgaaact cttccatctg ttccaaaacc atctgaaaat ccttgacctc ttgcacctgg
    11101 taaccccagt ttatcaagtt tttagcaact agttggctaa tcgttttatc atcctcgata
    11161 agaagaattt tctgcataac tatctccttt catatcacta ctatttatta tagcttacat
    11221 tggaacaaat ttgagtattt ttatacattt acacaagaag tagactataa aaccaggtca
    11281 tcagtggtgc agatttgtca gaccaatcat atttcgttat actagataaa aaaggaaagg
    11341 actgctatgc cgaatttatt aacctatatt gaagaaactc aatacgataa tttttatgat
    11401 aaacctatca ataagctaga tattctggct ctgaccgaac tttcctacct cccttttgat
    11461 aatctggtcc cctactcttt tacagaaaat ggtgtacgac ttgatcggtt ggcagcgacc
    11521 tttgaagaga cctacaaaaa taatttccct cccttttcta tggtgaccaa aaaccgccta
    11581 gccttactag gacttctagc caaatccata cgcttcaaat ctatcaaggc atttggcttc
    11641 gttgacgatt atcagttgga acaggaaaag cagttttcag caattagtta tcgcattgac
    11701 cgaaaaacga ttgtgacttg cttcagaggg acagatgata ctattatcgg ttggaaggaa
    11761 gatttccaca tgacctatat ggatgaaatt cctgcccaac gtgcagcgag tggctactta
    11821 gaaaaactta tgatgcagca ggggggccat ttttatctgg ccggtcattc gaaaggcgga
    11881 aatttagccc tctatgcagc atgccagcaa gctacagacc aacaggatag aatcctagct
    11941 atctatccct ttgattcacc tggcttacac aaaaaacact tggaagcacc tagttatcaa
    12001 gctattcacc aacggatttt ccctgtcatt ccccagaatt caatcgtcgg tatgatgtta
    12061 gaaacaccag aaaatgttca gattgtccag agtaataccc ttggcattct acaacatatc
    12121 agtttttctt gggaggttga cggaagcgac ttcaaaccag cccctgccct gaccagcgat
    12181 agcctacaaa cagaccaagc cttaaaaact tggacggcta gtctaacaga cgatgaatta
    12241 cgagacttct tcgatttgtt tttcggcata tttatcaaag caggaattga acgttttagc
    12301 gacatcacgg taaatcccct acagaaatta caggaaattg atcgactgag aaaagaaata
    12361 gctccagaag aagctgaaat ggtggacaaa ctcattcggc tcctctttga tacccgttat
    12421 caaatctgga gggacaatat tccaagccct gaaatctctc taccagattg gcggaaactt
    12481 ttccaaagaa atacaactga aaataaagaa aactagctca accacctgag ctagttttct
    12541 ttatcaaatc ttcttaaaaa ttgagataat ttttctaatc ctttgattag agtttcttgt
    12601 tcacaggcaa aacctagacg gacatagccg tcacgttcaa accgattacc aggaactagg
    12661 agaacaccgt gttcttgcaa gagttgcaag caaaaatctt ccataggcat atccactgca
    12721 atcttgacaa agctagtgga aacaaccgct ggacggatat aggaaacgag gggttcttcc
    12781 tcaatccatt ggtccaaaat tgctaaattt tcttctaaaa tgtgccgatt tcgttctaga
    12841 atttctttat aatgggccaa agctaattga gccaccagat cgtcaaagac acctgcacaa
    12901 atcatagtgt aatcccgata atctctcaga atgtccgtca cttgatgatt ggcggccacc
    12961 caaccaatac gaataccagg cagggaatag gtcttggata agctatttac agcaattcct
    13021 ttatcataga cttctataat agatggcaca tccagtccag aaaaggaacg atagacttca
    13081 tctgatagga tgtaagcacc aacttcacta gcaatttccg ccaattcttc caaataaacc
    13141 ctatccatga cagcgccagt tggattattg gcattgttga tgcaaatcat cttagtgtta
    13201 ggacgaatga gctgacgcag tttttccaag tctggcaacc atccatgctc ttcctcaatc
    13261 tgccacaaat ccacttctgc tcccaaagac tttggaatat cgtagagttg ttggtaggtt
    13321 gggtagagag aaatgacgtg gtcacctggc tcaatcaaac tgtaaagaac taataagttg
    13381 gcccctgttg ccccattggt ttggaggatt tgttcgggac tgacctgttc gtagagctga
    13441 ctgacagcct ctttaaatgc tggcgaacct tctatccagc catagttgag cttggtgccc
    13501 tgcaatttct tataaaaatc ctcaggattg gtgcccgaca aggcaaacaa ttcttccaaa
    13561 gttagagcag aaatggacac tcctgcaatg tcataggtgg cgctagtttc atggacattc
    13621 agccattcct caacgccaaa acgtggtaat ttcatcagtc ttcctacttc aatttattct
    13681 gttcttattg taccatattc tcacaataat tgtataaata tgcgatatat cataaaagga
    13741 atatttttac aattgaaaaa gcagactttc tcaaacgaga ctgtctgctt ctctttttcc
    13801 cctcttaaaa cttccctaaa ttcgtcaagt cttttttgtc tggcatgact tccttgttgt
    13861 cccaacgctc gacaatctta ccatctttga ggcggtagat gtcaaagtgg gcgtagtcct
    13921 gacctgcaat ccagactttc gaataagcca ccacatagtc accctgtccc atcaccttga
    13981 agacaaagtc gtagttaaca gccttatcga ccagatagtt tttataggcc gcaccgcctt
    14041 gggcaatctc ttggttgtgc tgaatcaggt ctgccgccac atagtcgtta aacttctcga
    14101 tttccccatt ttgaaggaca tcaaccaaga aacgacggac gattttcttg ttgtcctcag
    14161 tcttgtctag gtctgtcaac tcaaagtcgg cataaatcgg gtctgtttga ccaatggttt
    14221 tgggataggc atcgatgaca tcccaatgct cgacgatacg gccgttttca tccgcacgaa
    14281 agatgtccgc cgtcacccac tcagcctcac cgtcattgag tttcttgtgg acatggacga
    14341 agacaaaatt tccgtcttca atggcacgaa caatggtgat ttcccgcttg ggattgcgtt
    14401 tgaaaaaatc ctcaaagaaa gtcgcaaagc cctctttctc atcaggtaca cccgtactgt
    14461 gctgggtata gctgactccc atatagtttt catgcacttc cttgatttcc ccgtcccgaa
    14521 tgccacggag atagagattt ttagcatttt ctagttgatt agtcatgatt ttctcctatt
    14581 ttagtagatt ttctttctcg aggtattccc tggccacctg ctctgccgac ttgccttcca
    14641 catcgaccgc atagttcatc tgggtcattt cctcggtcga gatgttgccc gccaaatccc
    14701 ctaggacttg ctccaactcg ggatatttgt ctaaggtttc tttggtcaat agcggagcgg
    14761 cctgataggg tgggaagagc tctttgtcgt cttccaggat tttcagcttg taagaaacaa
    14821 tcttgctatc cgttgagtag gcatcaacta gctgcacatc tccattttta atagcttcgt
    14881 agcgaagagc aggctccatg gttttgacat taagattgag cccgtaaaga ttggtcagac
    14941 cgacattccc atcctcacga tcattaaact ccaaagagaa gcctgcgaca gctgtctgct
    15001 gcacttttgc caaatccgaa atcttttcaa gaccatgatc ttgagcatag tcttctgtca
    15061 cagccaaagc ataggtattc tggaaagcca ttggtgctaa atacatcaga ccatcctgtt
    15121 ccaaaatcgc ttcttttgcg taggtataaa cctcctcagg attgttggac aggtcagacg
    15181 gtggattggt caatagtgtc gtcgtaatgg taccagtgta ctcagggtaa atatcaatcg
    15241 agcctgattt gagggcttca tagaggaagc tggtcttgcc aaagtttggc tccagctcca
    15301 cctcgatatc cgtctggtct tcaatcaaaa gcttgtacat attgatgagg atttcaggct
    15361 ctgcccctag tttcccagcg atgactagct tgttcgatga cgattggttg agcggaacat
    15421 agctagctcc gactgccaaa agtgtcacca agagagcggc tagaatggtg cgaggcttct
    15481 tgtcctgcaa aaccttgatg actgagccaa agaggacagc caagactgcc gacgacaccg
    15541 ccccaatcaa aatcagggcc gagtcattgc ggtcaattcc caagaggata aaggagccta
    15601 gaccacctgc accgaccaag gcagcaagcg ttgccgttcc aataatcata atggacgccg
    15661 tccgaatacc agacatgaga atgggcatgg ccagagccaa ctggaattta cgcaattttt
    15721 ccatcttggt cattccaaag gctgtcgcag cctcttccag cgagggatcg atttccgaaa
    15781 gtccggtcaa agttccctgc aaaatgggga aaatggcata aataaccaga gccaccaccg
    15841 caggcagcgt tccgataccc ataaaaggaa tgaagagccc cagaagtgcc aaggagggaa
    15901 tggtctggaa aacgcctgtc acctgcaaac tccactcagt cagctttttc ttgttagaca
    15961 agaccaagcc cagtggcacc gcaatggcaa tagcaataat caaggccaga agggaaatcc
    16021 gcaggtgctc aaagagggct gtcagccagt ccgccttgcg ttccagaaag gttgttacca
    16081 actgatccat taggcacctc cccgattttt cataaattca acaacaaagt cattagcagg
    16141 ctggttgcgg agctcatcag gcgtgcccaa ctggaccact tctcctacct tcatcaagca
    16201 gatacggtct gccaacttaa ccgcttcgtc catatcatgc gtgacgaaaa ccgttgtcat
    16261 cttgaactct tcatgcaagc ctttgattaa gtcctgcaac tgccccttac taatagggtc
    16321 tagggctgag aaaggctcat ccatcagcaa gaccttggga ttggcaatga tcgctcgcag
    16381 aatcccaatc cgctgttttt ctcctcctga caaatccttg ggcagacggt ccaaatagct
    16441 gtccggctca agacctacct tgttcaagag ttgtctggat tttttcaagg tttcttcctt
    16501 gttcaagccc ttcatctcag gaataagagc gatattttca gccactgtca tatttggaaa
    16561 taaagcaatc tgttgcagga cgtatccagt ttccaagcgt agcttacgca ggtcgaagtc
    16621 tttcagccgc ttgccctgaa tccgaatatc tccatctgtc tgctcaatca ggcggttaat
    16681 cagtttcaac gtcgttgttt ttccgctacc gcttggccca atcaggacaa agaattctcc
    16741 ctcttggatg tcaaaggtca aatttttcaa gattggcccg ttgacctgac aggtcaagga
    16801 cacattttgg tactcaatca ttcgtttctc ctcctaagat ttccgtcaag gcctgtccat
    16861 agcgaccaaa gaggtctagt aactgctcct gttcttccaa gctgaactgg gacagggctg
    16921 ttttttccgc ctcttccaag gggtccaaaa tccgactggc atagacacga ccttcctcgg
    16981 tcaatttgac cttcttatgc cgtttgtctg ctggattttc ctcaaaaaag acatagcctt
    17041 tgtccagaaa tttcttaatg gtggcattga ccacctgctt actagagtaa gtcttcttac
    17101 tgatccaatt ctgcgtcaca cctctggata tataataaat aaacatcaaa atggataagg
    17161 acttgctgtt tagcccatgc ctccttgcat agtcctcata tagaccatcc tgattgtaaa
    17221 aaatccgagc aagtatctta ctccgctgtc tcaattctgt catcatcttc ctccctattc
    17281 agcacaatca ccttgccaaa agcctctggt ctactagtgt tcaaaacaac aacaattttc
    17341 acaacgactt cctcctgtaa tcatgttagt caatattttt tactatttta ccaaaaaaga
    17401 aaaatagtca atcattttga ctattttgaa acgtcaacta ccttattata gattcccgtc
    17461 ttcatccatt caaacatatt aaatccctaa aattatctgc ttttaattta cacaaaacaa
    17521 atgataatca gataacaaaa ctagcctaga aaattctaag ctagtttcat tttatccttc
    17581 aactgtttct gaggcagttt cgcttgctac ttctgaaaca gtttctgata ccacttcagt
    17641 ttgaacatct tcgatatcaa tttcaatatc ttcagacaca actacagcat cctcaatatc
    17701 ttcttctgtc aaatccactg tcggagcttt tgcagttttt gccttgatag cttccaaaat
    17761 gtcagccgtt gacaagtttt gttccgcaat tttttccttg atttgctcaa aggtttccaa
    17821 ggatttttcc ttaccagact tgaccaaatc ttctactgtc aattcaccag tttcaagttg
    17881 agtttttact tcggtaaatc gttcagtcgc ttgtttgcct aaatcttgtg ccttagtgac
    17941 aaaatcagca ttgatttctt catagttttc tttatagtca ttagcaaagt ttacaacttt
    18001 ttctttgact gttttaccag ttttacttgc tagaaaagca gctgcagcag ctcctcctgc
    18061 tgcccctaac aaaaggctag tccacacgct actagatttt tttaccattt ttttctcctt
    18121 tcttacctaa gagagatgag agagttgaca gagctgaaag agctccgcca gcttttacag
    18181 tacttgctcc agcagactta gctttgactg tcaaatcacg cgctgaagca ttcaaatcag
    18241 atacagaagt tgacaagtca gctactgcta caaaaagagg atcaagggtc gaaaacttgc
    18301 catttacatc ttcgactaaa acattggttt tagccaaaag ctcgttggtc tgatagagag
    18361 tcacattgac atcgctggtc aagaccttaa gcgtttgctg ggcttcatcc gttaccgtac
    18421 ccaatttttt caacaacaaa acaatatata ctgccaccgc cgcgatggac aaggcaataa
    18481 tcaagacaga aatttcaata atcatatctt ctcctttagg gttgatagaa tggaatagaa
    18541 gactttcttc tccgcaacac aaccatgata ataccaacga ggattaatac cccagatagc
    18601 cattgtgaaa cacgtattcc caagaacatg aggctgtccg tccgcaagcc ttctatcaaa
    18661 aggcgaccac agccatacca gactaggtag aaagctgtaa tttctccctg tttaaggaat
    18721 tttggccgtc tacgtaacac acaaaccaag ccaaagccta gcaaattcca gagagactca
    18781 aacaagaagg ttggttgacg gtaggcacca tcaatataca tctggtctcg gataaaggct
    18841 ggcaggtagt tcaaactttc tactgcttta ccataggcct cttggttaaa gaaatttccc
    18901 caacgaccaa ttgcttgagc aatcatgacc gatggcgcta caatatccaa aaaatccagg
    18961 gtattaataa agcgatactg ggtaaagaaa taaaggacga ttgcacctgt aatcaaacca
    19021 ccatagatgg caataccacc gttccagata gcaaagatag ataaaatgtt atccttatat
    19081 tcactccatg aaaaggcgac gtagtaaatc cgtgccccta tgatagaaag cggaaatgcg
    19141 attagaataa aatctaaaat atcatcctga cgaattttct ttcgcggacc ctcttttgtc
    19201 gccagataaa cacctagaat cagaccaagc aaaatgcaga ttgcatacca acgaatttct
    19261 aagggaccta atttaatcgc aataggatcc atattaagcc tcttctctat tgtgttcaat
    19321 taaattagac aagcgttctt cgaagatctt cgttgcatca aaccccatct gtttggcacg
    19381 gtagttcatg gctgcagctt caattacgac agaaatattt cgaccggttt ttacaggaat
    19441 acgtatttgt ggaatagcaa ttcctgctat ttcgatagta tcgccagagt ttcccaaacg
    19501 gtcaaatacc ttacctgttt caaaattttc aagatagaca gctaattgca cttctgatga
    19561 atctttcacg gcactagcac cgtatagact catgatatcg ataatgccga ctcctcgaat
    19621 ctctaataag tggcgcagaa tttccgcagg ttcaccccag agagtaacat cgtcttttgc
    19681 atagacatca acacggtcat cagccaccaa tcggtgtcct cgtttaacca actcaagacc
    19741 agtctcactc ttaccgatac cagaatcacc ctggataagc acacccattc cgtaaatgtc
    19801 catcaagaca ccgtgtacac ttgtccgctg agccaggcga ctatccaagt aagaagacaa
    19861 ttctcccgat agacgactgg ttgctgtctt gcttcggcag atagcaattt gtttttcttt
    19921 tgcagccttg tacatctcct cagggatttc caagccacgt gccactatga caacaggggt
    19981 ttctggctgg aacatctgag aaagaacctg atagcggtta tgggcagtca tggccatcaa
    20041 ataagaccac tccttcatcc ccatcaactg gatgcgctct ggagcatagt aatcaaaata
    20101 gccagtcatt tccaatcctg gtcgggtaat atctgccgtc gtaatttgct tttgcaggag
    20161 ctcttccgta ctgtaagcac attcaatccg cagattatcg accaaatctt tcacataaac
    20221 ggtcatttct tccctcactt tcagttttat tatattatac cataagctag gggttggtgg
    20281 aaaaacaagt ggctgatttg gtaaaaagaa tgagtttctt cctttttctc acaaaattag
    20341 caaaacttcc acagattaaa tgcacttctc ttccattgct acata
//
LOCUS       DBNFEG010000015        19952 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.015, whole genome shotgun sequence.
ACCESSION   DBNFEG010000015 DBNFEG010000000
VERSION     DBNFEG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19952
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.015"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            2..1693
                     /locus_tag="AC1PNG_000385"
     CDS             2..1693
                     /locus_tag="AC1PNG_000385"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6700922.1"
                     /translation="MLGPVFKLLEACFELLVPLIIAYIVDTIIPNGSQGNLVAMLFLL
                     VGLACIGIIVSLIAQYYSAKAAVGVTKELTNDLYQKVLSLPKSSRDILSSSSLLTRLT
                     SDTLQIQTGINTFLRLFLRAPIVVFGSLIMAFYISPSLSAYFLEMIILLIFIVTVISV
                     MTSRIYQSMRKELDGLVGQVRETVTGWRVIRAFGQREREIKAFQGINQIYKKQQLQAG
                     FWSSLLSPLTFLVVNGTLLILIWQGNIAISHNLLEQGMLVALINYLLQILVELVKMIM
                     VVSTLNQTYISAQRIQEVFDQTSEDVESSLPKVVSEDKEIIFSVRHISFSYPKSAEES
                     LSDIAFDLRKGQFMGIIGGTGSGKSTLVDLLQALYSVPTNQLSLFIDGKSPKNLKEWR
                     QQIAIVPQQAQLFAGTVRSNLSLGLESVLDSELWAALEIAQAKSFIEDKGGLDSPVEA
                     FGKNFSGGQRQRLTIARAILQKAPILVLDDATSALDYLTESRLLAAIRRAFPAQTLIM
                     VSQRTNSLRTADQILVFDQGRQVGLGRHEDLLRSSAIYQEIDQSQHREEDSHETI"
     gene            1680..3422
                     /locus_tag="AC1PNG_000386"
     CDS             1680..3422
                     /locus_tag="AC1PNG_000386"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6700923.1"
                     /translation="MKQSSFKELVRLVLGQPFQMYLMLVGTLVQVLLTVYLPILIGQA
                     VDAVLIADGQVLLGIIGKMVMVILLNTLVQWYLPLVTNRLVYGMVADLREQVYVKLHQ
                     MPLSYLDRHSVGDLVARFSSDSEQLTNGLLMVFNQFFIGILTIFLTIFTMARLDLTMM
                     LLVVALTPVSLLVARYIAKKSYGYYRKQTQARGKQSQLLEESISQLTLVQSFNAQEQF
                     TQGFQVVNDQYATYSQQAIFASSTVNPSTRFINAIIYALLAGLGALRIMAGQFTVGSL
                     TTFLNYASQYSKPFNDISSVLSELQSALACADRLFAILALDSIDDQAERKIDSEELKG
                     AISFDNVSFSYSPERSLIEHLDIDVEAGQKVAIVGPTGAGKSTMINLLMRFYDVTAGQ
                     IVLDGVPIRQYSREDLRRQIGMVLQETWIKSGTIHDNIAYGYPNATRELVIEAAKAAN
                     ADFFIRQLPQGYDTVLADGGEGLSQGQRQLLSIARVFVKIPKILILDEATSSIDTRTE
                     ILVQEAFAKLMEGRTSFIIAHRLSTIQSADLILVMVDGKIVEQGNHEALMAEQGVYYQ
                     MQTSQQTEEDESVR"
     gene            3406..3798
                     /locus_tag="AC1PNG_000387"
     CDS             3406..3798
                     /locus_tag="AC1PNG_000387"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIP6700924.1"
                     /translation="MKVLDKLNLQPITVLDDYQATMLNEKEVLLTTKVTEKSLNPYGM
                     AHGGFLFTLADSVAGLTTVAGGSYSVTLQSNIHYMKAAKLGDTLSVTGSCTHDGSRTK
                     VVEVKIKNQDKQLLASASFTMFVTGKVE"
     gene            4033..5193
                     /gene="mutY"
                     /locus_tag="AC1PNG_000388"
     CDS             4033..5193
                     /gene="mutY"
                     /locus_tag="AC1PNG_000388"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIP6700925.1"
                     /translation="MLDLKEYGIEMWPEEKIQAFRKALLDWYDANKRDLPWRRTKDPY
                     AIWVSEIMLQQTRVDTVIPYYERFLHYLPTISDLAQAPEEVILKLWEGLGYYSRVRNM
                     QKAAQQMVEDFDGQFPTTHAAISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLSR
                     LFEVDYDIGLPANRKIFQAMMELLIDPERPGDFNQALMDLGSDIESPVNPRPQDSPVK
                     AFSAAYLNGTMDKYPIKAPKKKPIPVAYQGFLIRNKDNQFLLEKNNEAGLLSGFWSFP
                     LLEKGAIVEKQVSLFEVAEEVVQPDIRQSFTELYGLTVDWQEQEFGIVQHIFSHRKWQ
                     IEMVEGVAETLNLPESKELKWVSVEDFPTYPFAKPQQKMWENFIKEKEKQKS"
     gene            complement(5856..6656)
                     /locus_tag="AC1PNG_000389"
     CDS             complement(5856..6656)
                     /locus_tag="AC1PNG_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6700926.1"
                     /translation="MILGDILKEYRSKNKLSMDKFAELSGLTKGYISMLEKNQHPKTK
                     KALLPTMDTLEKVAKGMAISVGELIEQLDSNQDIALTITPSQFKLFQQPIHPEVQTLD
                     NELKGDFHAQWLTFGQEQLAAMQTTSITPSASILHLDDYRERIDLPVPGKVSAGTGYW
                     QDTDMNNLVSFYNDEIPDNQSYDTIAQVVGDSMAPHIEDGDYLFIRLTPHIPLNTIGI
                     FSVNGENFVKKFKGDYLQSLNSDYDDIYFSPEDDIRPIGQVVDIYSPD"
     gene            6788..7258
                     /locus_tag="AC1PNG_000390"
     CDS             6788..7258
                     /locus_tag="AC1PNG_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700927.1"
                     /translation="MKRMTEISWNDIYKEWETYANHFGLTTPINTENLRDQKSKDFGK
                     GSLITLDLLADYDTDSEKTAAIWVASFCRDLIQDYAYLLNGRAYLTVNQIYFQALKQF
                     QSGAVIWSKPLTRLQPKLFVSYRLLENLDLSHYSCVVELAMLQASMVRTQILEK"
     gene            7278..7820
                     /locus_tag="AC1PNG_000391"
     CDS             7278..7820
                     /locus_tag="AC1PNG_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700928.1"
                     /translation="MFNLLNKKAEVSKVAEYWNDTLIERGILSADELLEGKCWRCKSS
                     HGVAVCQIVSSKWSKDASLANQMVLCLSCQHEKPNVADTEIVWQWLEVENDERYWTLQ
                     GMAEYEKMYKKSVLQELWDMGIRDGEEVETLVNKVTSLSRKNDIVLNRATLAGLFRCE
                     IEQMRRKAFLNWTGIFKLVS"
     gene            complement(8046..9077)
                     /locus_tag="AC1PNG_000392"
     CDS             complement(8046..9077)
                     /locus_tag="AC1PNG_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIP6700929.1"
                     /translation="MATITKRGNSYRATVSLYKKGEYKRETKTFSNRKDAELWTLEME
                     LEKGRGKNIAERSTLFPDFYRNWVHTVKKTDVREATFINYKRTLVIVDDLFDGIQLKH
                     LDDLVMQKKIDQYAETHSKKTVKELVLKIRGSLKYAYARGLISNDFGHLLKSKGQEQP
                     KSITEFKKLRQYCLSHTENEFNVLVALALETVARRGELLGIKKEDIFEYGIKILRSIS
                     PTNDDTRLKTKYSKRDISINEDVYQAVTKLAQTKEGYIFDWNGFKQASQLQKLLKQLG
                     LTKTTFHGLRDTHASFLFSKDISLDYISRHLGHNSILTTQQYYLELMPEKKHQQDADA
                     LSLLNDLSL"
     gene            complement(9169..9357)
                     /locus_tag="AC1PNG_000393"
     CDS             complement(9169..9357)
                     /locus_tag="AC1PNG_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6700930.1"
                     /translation="MLPQNNSPLLLNRQQAAELLGIDPKSFDKYIRSHPDFQCFMVGK
                     QERYLKSKLVKFIESHCD"
     gene            complement(9446..10201)
                     /locus_tag="AC1PNG_000394"
     CDS             complement(9446..10201)
                     /locus_tag="AC1PNG_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700931.1"
                     /translation="MALGNVEKDTEGWIELINQYLQYCIEIGLSPYTQATYKVALTKV
                     LGVSSTNFIATQPRTRANRMNNRVLHKDYRLSNKNNDYWHKVVTSTGLRKSELIHVTG
                     DALQRGRDGRWYLNLAGHKHHTKGRRDRWSPIMATSQEEEEWLVAIFQRAGEKKVFHV
                     PKDLILDDFDGKKVPTALKSHKYPTEYAERVYRSVAREISKIRNRKEVIHLRKELVGI
                     SLNRKACKIVIKTLGHNRPEEFPRSYAYILLKR"
     gene            10480..11247
                     /locus_tag="AC1PNG_000395"
     CDS             10480..11247
                     /locus_tag="AC1PNG_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIP6700932.1"
                     /translation="MNKRERLEEITRLVNKKGTVRISEIVELLNVTDMTVRRDFIELE
                     EQGVLTKIHGGARSNKAFQYREYSHSEKHIQNKEAKQEIAERAAQLIEDGDTVFLGPG
                     TTVAFLAEAIKNSRLTVITNCMPVFTILSQKKTEEFQVFLLGGEYRQVTESFVGEITN
                     TSLEKMRFSKIFFSGNGLKGTDVMTSTLAEAYTQNIAIKHSLEKYLLLDASKIGKDDF
                     TSFCDLHDVTALVTDMNAKDENYQLLSKHIEIITTKL"
     gene            11453..11875
                     /gene="lacA"
                     /locus_tag="AC1PNG_000396"
     CDS             11453..11875
                     /gene="lacA"
                     /locus_tag="AC1PNG_000396"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HIP6700933.1"
                     /translation="MTIIIGADPAGSKLKDVIKDYLLEKSYDFKDVSDGSDFVDTTLA
                     VVAEVNQQEGNLGIVIDAYGVGPFMTATKVKGMIAAEVSDERSAYMTRGHNNSRIITI
                     GSEIVGEGIAKNIVKGFVEGEYDGGRHQIRVDMLNKMC"
     gene            11902..12417
                     /gene="lacB"
                     /locus_tag="AC1PNG_000397"
     CDS             11902..12417
                     /gene="lacB"
                     /locus_tag="AC1PNG_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HIP6700934.1"
                     /translation="MKIAIGCDHIVTYEKIAVVDYLKNQGYEVLDFGTYDNVRTHYPI
                     FGKKVGEAVASGQADLGICICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGKITGELLMCDIIEAFIKAEYKPTEANKRLIEKIAAVETLNQEQANPEFFTEF
                     LEKWDRGEYQD"
     gene            12427..13359
                     /locus_tag="AC1PNG_000398"
     CDS             12427..13359
                     /locus_tag="AC1PNG_000398"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HIP6700935.1"
                     /translation="MILTITLNPSVDIAYQLDTFHLDTVNRVEKVQKTAGGKGLNVTR
                     VLKQIGEDVVATGFIGGEIGSYVKKQLTRNDIKNSFVEIGSETRNCIAVLHDGKQTEI
                     LEQGPTIQEHEALNFIEHLEIILNNVDVVVISGSLPKGLASNYYVEIVELCKKCGVAV
                     VLDCSGEALKNVLESQQKPTVIKPNTEELSQLIGKEVTDDIQELKSVLSGQLFQGIDW
                     IVVSLGAQGAFAKYKDKFYRVRIPKIKVVNPVGSGDSTVAGIAAGLVHALPEAELLKN
                     ANVLGMLNAQEEQTGYVNLVHSESLYSQIEVEEV"
     gene            13361..14338
                     /gene="lacD"
                     /locus_tag="AC1PNG_000399"
     CDS             13361..14338
                     /gene="lacD"
                     /locus_tag="AC1PNG_000399"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HIP6700936.1"
                     /translation="MVVTADKRKYMEKVRNEAGIISALAFDQRGALKKMMAKHQTEEP
                     TVEQMEELKRLVSEELTQFSSSILLDPEFGLPASRARNEQCGLLLAYEKTGYDTSSTS
                     RLPDCLVEWSVKRLKEEGADAIKFLLYYDVDGDPYVNLQKQAYIERLGSECQAEGLPF
                     FLEILSYDETIEDNASVEFAKVKPRKVNEAMRVFSDERFGVDVLKVEVPVNMNFVEGF
                     GTGEVVYTKEEAAEYFRQQEASTNLPYIYLSAGVSAKLFQDTLVFAHEAGAKFNGVLC
                     GRATWAGVVPVYIEQGEEAAREWLRTVGRENIEGLDAVLSQTATSWLEK"
     gene            14527..15360
                     /locus_tag="AC1PNG_000400"
     CDS             14527..15360
                     /locus_tag="AC1PNG_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HIP6700937.1"
                     /translation="MFRIIQSLNNNAALVKNEHGEQAVVMGLGITFQKKKGDLIVKDK
                     IENIFSLKNDEAKENFLTLLKDIPLDFISATYTVINHLVETYHYPVQEYLYVTLTDHI
                     YCAYQAVLKNTYQESRLPNISAEYPLEYKMAREALAMFREKLLNDFPNDEIGRIALHL
                     INAKGETIHPTSEEHDISKKIIAEVEQILIENGIKRSKENSNFYDRFMIHLSYFLNYL
                     DRSHQSNESIASLEQYIKLQNPRAHQVGSQIFEMITSLVDEPVNDSERFYIVLHIQRL
                     L"
     gene            15392..15709
                     /locus_tag="AC1PNG_000401"
     CDS             15392..15709
                     /locus_tag="AC1PNG_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIP6700938.1"
                     /translation="MNREEITLLGFEIVAFAGDARSRLMEALAAAQKGDYAKAEELVE
                     AANACIVEAHHAQTSLLQKEAAGEDLAFSVTLMHGQDHLMTTLLLKDMMGHMIELYKR
                     GDK"
     gene            15709..17388
                     /locus_tag="AC1PNG_000402"
     CDS             15709..17388
                     /locus_tag="AC1PNG_000402"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HIP6700939.1"
                     /translation="MNKLIEFIEKGKPFFEKISRNPYLRAIRDGFIAAMPVILFSTLF
                     LMVAFVPNIFGFTWSDEAVAAIMKPYGYTMGIVAVLVAGTTAKSLTDAFNRQLPKTNQ
                     INFISTMIASISGFLLLASDGIEGGFANGYMGTKGLLTAFLAAFITVNIYKVCVKNNV
                     TIRMPDEVPPNVSQAFKNVIPYALSIFVLYGIDLVTRQFLGTNVAEAILKLFEPLFTA
                     ADGYVGITIIFGAYALFWFVGIHGPSIVEPAIAAITYANIETNFQLLQAGQHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPLV
                     LNPVFFVPFILAPIANVWIFKFFVDTLKMNSFSVNLPWTTPGPLGIVMGTNFAPLAFA
                     LAILLVVVDVLIYYPFLKVYDEQILAEEQSGKVENSLKEKVAANFNTAKADAILEKAA
                     VETEISEQTNVLVLCAGGGTSGLLANALTKAAKEYGVPVTATAGSYGAHREILPEYQL
                     VILAPQVASNYDDMKQETDALGIKLAKTEGAQYIKLTRDGQGALDFVKQQF"
     gene            17624..19030
                     /gene="lacG"
                     /locus_tag="AC1PNG_000403"
     CDS             17624..19030
                     /gene="lacG"
                     /locus_tag="AC1PNG_000403"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HIP6700940.1"
                     /translation="MVKTLPKDFIFGGATAAYQAEGATQTDGKGRVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLELSEQFGVNGIRISIAWSRIFPTGFGAVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPETLHSNGDFLNRENIEHFVNYAAFCFKEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHAKAVKLYKDAGYSGEIGVVHAL
                     PTKYPYDPSNPDDVRAAELEDIIHNKFILDATYLGYYSEKTLEGVRHILKVNGGELDL
                     RDEDFAALDAAKDLNDFLGINYYMSDWMRAHDGETEIIHNGKGEKGSSKYQIKGVGRR
                     ESPVDIPKTDWDWIIYPQGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDGTVYDD
                     GRIDYVKKHLEVISDAISDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAYWYKKVAETQVIE"
     gene            19095..>19952
                     /locus_tag="AC1PNG_000404"
     CDS             19095..>19952
                     /locus_tag="AC1PNG_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HIP6700941.1"
                     /translation="MLELKNENLTVQFSELGGQIISIKDKDGIEYLWQGDPTYWSGQA
                     PVLFPICGSLRNDWAIYEPAERPTFTGTIPRHGLVRKMLFKNVKNTENSLEFSISSNE
                     ETVKNYPFEFELSINYSLFGKTIRTEYRMKNLEGHRKLPYFIGGHPGFNCPLLDGESY
                     EDYYLEFEKVESCTVPRSFPETGLLDLRDRRPFLENQKTLDLSYKLFEHDAITLDQLA
                     SRKVTLKSKNHQKKLSIAFDDFPYLVIWSTTNQSPFIALEPWSGLSTSLEESDIFNAK
                     RNISYVAPKE"
BASE COUNT         6147 a         3454 c         4242 g         6109 t
ORIGIN      
        1 catattaggt ccagttttta aactattgga agcttgtttt gagcttttgg taccattaat
       61 tatcgcctat atcgttgata caattattcc aaatggcagc cagggaaacc tggttgctat
      121 gcttttttta ctggttggtc tagcatgtat tggaattatt gtttcactaa tagcgcaata
      181 ttattcagct aaagcagcgg ttggagtgac taaagagctg acaaatgact tgtatcagaa
      241 ggttctttcc cttcccaagt ctagccgaga tattctttcc tcatccagct tgctgacacg
      301 attgaccagc gacactttac aaattcaaac aggtattaat acttttcttc gtttgttttt
      361 acgtgctcca attgtcgtat ttggttcgct catcatggca ttctatatta gcccaagtct
      421 atctgcctat tttctagaaa tgattatcct attgattttt atagtgacag tcatttctgt
      481 tatgaccagc cgtatttatc aatcgatgcg aaaagaatta gatggtttgg tggggcaggt
      541 acgtgagaca gtgactggtt ggcgagtgat tcgtgcattt ggacaaagag agcgggaaat
      601 taaggctttt caaggcataa atcagattta taagaagcaa cagttgcagg ctggtttttg
      661 gtctagtctt ttatcgccat tgacattttt agtggtcaac ggcaccttgc tcattctcat
      721 ctggcaggga aatatagcca tttcacacaa tttgttggaa caagggatgt tggtcgctct
      781 catcaactac cttttacaaa ttttagtaga attagtcaag atgattatgg tcgtgtctac
      841 tctcaaccag acctatattt cagcgcaacg tattcaagag gtttttgacc aaacatctga
      901 ggatgtagag tctagtttgc caaaagtggt tagtgaggat aaggaaatca tcttttctgt
      961 gcgtcatatt tccttttctt atccaaaatc tgctgaggag tccctgtctg acattgcttt
     1021 tgacttgcgt aagggacagt ttatgggaat tatcggggga actgggtcag gtaagtccac
     1081 cctagtagat ttattgcagg cactctattc agtgccaaca aatcagctct ctctcttcat
     1141 tgatggaaaa agtcctaaga atttaaaaga atggcggcag caaattgcca ttgttccgca
     1201 acaggctcag ctttttgctg ggactgtgcg ttctaacctg tcattaggct tagaaagtgt
     1261 attggacagt gagttatggg cagcgctaga aatcgcacag gctaagtcct tcattgagga
     1321 caaaggaggc ttggatagtc ctgtcgaagc ctttgggaag aacttttctg gtggacagcg
     1381 tcaacgtttg accattgcac gcgctatttt acagaaagca ccgatattgg ttttagatga
     1441 cgcgacctct gctttggatt atctgacaga gagtcggtta ttggccgcga ttcgtcgtgc
     1501 atttcctgct caaaccttaa taatggtatc tcagagaacc aatagtttac gaacagctga
     1561 tcagattttg gtctttgatc aaggccgtca agtgggactt ggccgtcatg aagacttgct
     1621 gagaagctct gccatttacc aagaaatcga tcaatcacaa catagggagg aggacagcca
     1681 tgaaacaatc tagttttaag gaactggtca gattggtttt aggccaaccc tttcaaatgt
     1741 acttgatgtt ggtgggaact ttagtgcagg tgcttctgac agtttatctg cctatcttga
     1801 ttggtcaggc agttgatgca gtattgatag cggatggtca ggtcttgcta gggataattg
     1861 gcaaaatggt gatggttatc ctgcttaata cgctggtgca atggtatctc ccgctagtaa
     1921 ccaatcgctt ggtttatgga atggtggctg acttgcgtga acaggtttat gttaagctgc
     1981 accagatgcc cctatcttat cttgatcgtc attcagttgg tgatttagta gctcgttttt
     2041 caagtgatag tgagcaatta accaacggac tcttaatggt tttcaatcaa tttttcatag
     2101 gtatcttgac cattttccta accattttca ctatggctag gcttgacttg acgatgatgt
     2161 tgctggttgt ggcattgact ccagtttctc tgcttgttgc acgctatatc gctaagaaat
     2221 cctatggtta ttatagaaaa caaacacagg cacgtgggaa gcaatctcag ctacttgagg
     2281 aatcgatcag tcaattgacc ttggttcaat ctttcaatgc ccaagagcaa tttacgcaag
     2341 gttttcaagt tgtgaatgac cagtatgcga cctattccca acaggctatc tttgcctctt
     2401 caacggtcaa tccaagcacg cggtttatca atgctattat ttatgcctta cttgcaggac
     2461 ttggagcgct gagaattatg gcagggcagt ttacagtggg ttcactgaca acttttctca
     2521 attatgctag tcagtacagc aagcccttta atgatatttc ctcggtccta tcggaattgc
     2581 agagcgcctt agcttgtgca gacaggctgt ttgctatctt ggctttagat agcattgacg
     2641 atcaggctga gcggaaaatt gattcagagg agttgaaggg agctatttcc tttgacaatg
     2701 tgtctttctc atatagccct gaacgttctc tgatagaaca tttggacatt gatgtcgaag
     2761 ctggacaaaa ggtagccatt gttggaccta ctggtgccgg taaatcaacc atgattaacc
     2821 tgcttatgcg cttctacgat gtgacggctg gtcagattgt tttagatggg gttccgatta
     2881 gacagtacag tcgtgaggat cttcgcagac agattgggat ggttttacag gagacatgga
     2941 tcaaatctgg taccattcat gataatattg cctatggcta tccaaatgcc acgcgggaat
     3001 tggtcattga ggcggctaag gcagctaatg cagacttctt tattcgtcag cttccgcaag
     3061 ggtatgatac agttttggca gatggaggag aaggcttatc ccaagggcaa cgtcagctct
     3121 tgtctattgc gcgtgtcttt gtcaagatac ctaaaatcct gattttagat gaggcgactt
     3181 cctctattga tacacggaca gaaattttag tacaagaagc atttgccaaa ttgatggaag
     3241 gacggaccag ctttattatt gctcatcgcc tatcaaccat tcaatcagct gacttgattt
     3301 tggtcatggt ggatggaaaa attgttgaac aagggaatca tgaagcgttg atggctgaac
     3361 aaggcgttta ttatcaaatg caaacaagtc agcagacaga ggaggatgaa agtgttagat
     3421 aaattaaact tacaaccgat tacggtttta gatgactatc aagctacaat gctcaatgag
     3481 aaagaggtgc tactgaccac aaaggtgacg gaaaaatctc tcaatccata tggtatggct
     3541 catggaggct tcctctttac tttagcagat tcagtagcag gattgacaac agtggctggg
     3601 ggttcttatt ctgtcaccct gcagtccaat atccattata tgaaggcggc aaaacttggt
     3661 gataccttat ccgtcactgg ttcttgcaca catgatggaa gtagaacaaa ggtcgttgaa
     3721 gtgaagataa aaaatcaaga taaacagcta cttgcttcag caagctttac catgtttgta
     3781 acaggaaaag tagaataaaa ctcttcgaac atcaaaagca gacagtcttg gctagattga
     3841 gagaaaaaaa cttcgttcgc tctatttcca acctcccaag tttccccaaa cctttgtaga
     3901 aatgaacttc gttcgctcaa tttccaacct ccaaaggttc cccaaacctt tggagctagt
     3961 ttgattttga ttataaacga tataagggag ttggcaaaag ctggctccct ttctttgtgt
     4021 tatactagat gtatgttaga tttgaaagaa tatggaatcg aaatgtggcc ggaggaaaag
     4081 attcaagctt ttcgcaaggc actcttagat tggtatgatg ccaataaacg tgacttgcct
     4141 tggagacgga ccaaggatcc gtatgctata tgggtttctg aaatcatgct tcaacagaca
     4201 agagtagaca cggtcattcc ttattatgaa cgttttctcc attacttgcc tacgattagt
     4261 gatttagcac aagcaccaga ggaggtcatt ctcaaactct gggaaggatt gggctattat
     4321 tcgcgcgtgc ggaatatgca aaaggcagct cagcaaatgg tagaagattt tgatgggcaa
     4381 tttccgacta cgcatgcagc catttcaagc ctaaagggca ttggtcccta tactgctgga
     4441 gcaatttcca gtattgcttt taatttgcca gaaccagcgg tagatggcaa tgtgatgcgg
     4501 gttctcagcc gcttgtttga ggttgattat gacattggtc ttcctgccaa tcgtaagatt
     4561 ttccaagcga tgatggagct attgattgat ccagagcgac cgggcgattt caaccaagcc
     4621 ctaatggatc tagggtcgga tattgaatcg ccagttaatc cacgtccaca ggacagccca
     4681 gtcaaagcct ttagcgcagc ctacttaaac ggtacaatgg ataagtatcc aatcaaagca
     4741 ccaaagaaaa agccgattcc cgtggcctac cagggatttc tcatcaggaa taaagacaat
     4801 caatttttat tggaaaaaaa taatgaagca ggtcttctat caggtttctg gtcttttccg
     4861 cttttggaaa aaggagcaat cgttgaaaaa caagtctctc tctttgaagt tgcagaagaa
     4921 gttgttcagc cagatattcg gcagagcttt acggaacttt atggtctgac tgttgattgg
     4981 caagagcaag aatttggcat cgtccagcac atttttagtc accgcaaatg gcagatagaa
     5041 atggtagaag gtgtggcaga aacacttaac cttccagaat caaaagaatt gaaatgggtt
     5101 tctgtagaag attttcctac ctacccattt gctaagccgc aacaaaaaat gtgggaaaac
     5161 tttatcaagg aaaaagaaaa gcaaaaaagt tgactttgga gcacataatt atttgaaagc
     5221 gttttacaat agtggtataa tctaattgaa aaaaggaaat agttagttaa gatgttgaac
     5281 gccatctgaa ttgctgaaag gagttcttta gcgttcgtag aagtgtatat actattagcc
     5341 atctttctct aataaaaaga gagggatgct agtaggggaa aagtccgtta tccctgtttt
     5401 atatctattt gtgatatcca ttgtcaaatg catagcatta actcattatt tagccttgct
     5461 acccggttca atcagaagta ggctaagatt attcgtgtcg taactactat gtgttcaaga
     5521 tgttgtaact tttgaacata actttagaaa atcacaaaaa cctctagcga ttttcatcat
     5581 aataagcaat ctccacactt tagacaaata gtaagaggac aatcaaagac ataagggtag
     5641 cagaataatt atttaatcat taacttgttg tcaaataact atctggtaat ggacttttcc
     5701 aatttgacat ataaaaggga ggctgtcata gcctcctttt actgtgcttt tcagtttgtt
     5761 ttcagtagga aaaaacgcca gctatacgaa cagactttcc aaaactagat ggatgaatgt
     5821 agccaatata actagtgatt aactaggctt agaaatcaat ctggactata aatatctaca
     5881 acttgaccaa taggtcgaat atcgtcttct gggctaaaat agatatcatc atagtcagaa
     5941 ttgagtgatt gcaaatagtc acctttgaac tttttaacaa aattttcccc attgactgaa
     6001 aaaattccaa tcgtatttag aggaatatga ggagttaggc gaatgaagag atagtctcca
     6061 tcctcgatat gcggagccat agaatctccc acaacctgag cgattgtatc ataagattgg
     6121 ttatctggaa tctcatcgtt atagaatgat acaagattgt tcatatctgt atcctgccag
     6181 taacctgtac cggcagaaac cttaccagga acaggtaaat cgattcgctc tcgatagtca
     6241 tccaagtgaa gaatagaagc agaaggagta attgaggtag tttgcatagc tgctaactgt
     6301 tcttgaccaa aagttaacca ttgtgcatgg aaatcacctt ttaattcatt gtccaaggtt
     6361 tgaacctctg gatgaatagg ctgttgaaag agtttaaact gggaaggagt aatcgttaaa
     6421 gcaatatcct gattagaatc tagttgctcg attagctcac caaccgatat agccatgccc
     6481 ttagcgactt tttctagcgt atccatcgtt ggcaggagag cttttttggt tttgggatgc
     6541 tggttctttt ccaacataga aatataacct tttgttaaac cagataattc agcgaattta
     6601 tccattgata atttattctt gcttcggtat tcttttaaaa tatcacctaa aatcatggtt
     6661 gtctccttat ctgtttaacc aattatacca ttaaaaaata ttttttgcaa attttgttta
     6721 acacgtttga caaaaaatca aaacggtgtt atgataatct cacaaaaaag aaaaatagga
     6781 gtcgcaaatg aaaaggatga cagagatttc ttggaatgat atttataaag agtgggaaac
     6841 ctatgctaat cattttggtt tgacgactcc gataaatact gaaaatttgc gagatcagaa
     6901 atcgaaggat tttggaaaag gaagtctgat tacgctggac ttattagccg attacgatac
     6961 tgattctgag aagaccgcag ctatttgggt agcttcattt tgtagagatt taatacaaga
     7021 ttacgcatat ttactaaatg gtagagccta tcttacagtc aatcagattt attttcaggc
     7081 actcaaacaa tttcagtcag gagcggtaat atggtcaaaa cctctcacac gattgcaacc
     7141 gaagttattt gtatcttatc gtttgttgga aaatcttgat ttgagtcatt attcttgtgt
     7201 agttgagtta gcaatgttgc aggctagtat ggttcggaca cagatattag aaaagtaatt
     7261 atagtaggag aatgaaaatg tttaatttgt tgaataagaa agctgaagta tcgaaagttg
     7321 cagagtattg gaatgatact ttgatagaaa ggggaatttt gagcgctgat gaattgcttg
     7381 aaggaaaatg ctggagatgt aaaagttctc atggtgtagc tgtgtgtcag attgtatcaa
     7441 gcaaatggag caaggatgcg agcctggcta atcagatggt cctctgtcta tcctgccagc
     7501 atgaaaaacc taatgtagct gatacagaaa ttgtgtggca atggttagaa gttgagaatg
     7561 atgaacgtta ctggactcta caaggtatgg cagaatatga gaaaatgtat aaaaaatcag
     7621 tacttcaaga gttatgggat atgggaatta gagatggtga agaagtcgag acgcttgtaa
     7681 ataaagtaac tagcctttct cgtaagaatg atattgtttt aaatagagca accttagcag
     7741 gattatttcg ctgtgaaatt gaacaaatgc gtcgaaaggc atttctaaat tggacaggaa
     7801 tttttaagct agtcagttaa ctgatgcagt tgtaggaaaa catctcatgc tgattggagt
     7861 aacagataaa taaaaagcgc atcagcttag aatcttaaag aaagaagtct aatgatgcgt
     7921 ttataggatt atatttcagc taataaaaat tggagtgaat gtttttgaat tatagctcaa
     7981 aaacgttagt caaaacctta ttataccaac gattatggcg cttttttggt gccagttggt
     8041 gttaattata atgataagtc gtttaagaga cttaaagcat cggcatcttg ctggtgcttt
     8101 ttttctggca tcaattctag ataatactgt tgtgttgtta agatagagtt atgacctaga
     8161 tgacgagaaa tatagtctag gctaatatct tttgaaaaga gaaatgaggc atgcgtgtca
     8221 cgaagtccat gaaatgtcgt ttttgttaat cccaactgtt ttaacagctt ttgaagttga
     8281 ctagcttgct tgaatccatt ccaatcaaag atatagcctt cttttgtttg tgcaagtttt
     8341 gtcacagctt gataaacatc ttcgttgatg gaaatatcac gcttagaata tttggtttta
     8401 agccgggtat cgtcattagt tggactaatt gatcgaagaa ttttgatacc atattcaaaa
     8461 atatcttctt ttttaattcc taaaagttct ccgcgccttg ctactgtctc aagggcgaga
     8521 gcaacaagaa cattaaattc attctcagta tgacttaggc agtattgtct gagtttttta
     8581 aattctgtga tagatttagg ttgttcttgt ccttttgatt tcaaaagatg accaaagtca
     8641 ttactaatca aaccacgagc atacgcatat tttaatgaac cacggatttt aagaacaagt
     8701 tcttttactg tttttttaga gtgggtttca gcgtattggt cgattttctt ttgcatgacg
     8761 agatcgtcaa ggtgtttaag ttgaatacca tcaaaaaggt catcaactat tacaagagtt
     8821 cgtttatagt taataaaggt ggcttcgcga acgtcagttt tcttgaccgt atggacccaa
     8881 tttctataaa aatcaggaaa taaggtagat cgttcggcaa tgttcttgcc acgacctttt
     8941 tcaagttcca tttctaatgt ccaaagctca gcatcttttc tgttgctaaa ggttttagtt
     9001 tctcgtttat attctccttt cttataaagt gatacagttg cacggtaaga gttaccacgt
     9061 tttgttattg ttgccatttt atcagccttt ctataaaaaa tgattgataa aatagactag
     9121 tcacatatag ggatatatga aaaatctaat tttatcaatc attatccgtc aatcgcagtg
     9181 gctttcaata aatttaacca gttttgactt caaatagcgc tcttgttttc ccaccataaa
     9241 gcattgaaag tctgggtgac ttctgatgta cttatcaaat gatttaggat ctattccaag
     9301 tagttccgct gcttgttgtc tatttaacag aagaggagaa ttattttgag gtaacatata
     9361 gaattccttt cattttctag atacaagtat tatgccacat gcgtaatgaa ttgaggctag
     9421 atttttgaaa attgttcaat gttacctaac gtttcaataa aatataagcg taggaacgag
     9481 gaaattcctc tggtctattg tgtcccagag tctttatcac aattttacaa gcttttctgt
     9541 tgagtgagat gccaactaac tctttacgca gatggataac ttctttacga tttctaatct
     9601 tggagatttc gcgtgctaca ctgcgataaa cacgctctgc atactctgta ggatatttgt
     9661 gtgactttaa tgctgttgga actttctttc catcaaaatc gtcgaggatt aaatctttgg
     9721 gaacatgaaa gactttcttt tctcctgctc gttggaaaat agcgactaac cactcttcct
     9781 cctcttgact agtggccatg attggagacc agcgatctct gcgtcctttt gtgtgatgct
     9841 tatggccggc taaatttaaa taccaacggc catcacggcc gcgttgcaga gcatcgccag
     9901 tgacgtggat taattcactt ttgcgtaagc ctgtcgaggt aactacttta tgccagtagt
     9961 cgttattttt gttggataaa cgatagtctt tatgtaatac acgattgttc atacgattcg
    10021 cacgagtacg tggttgtgta gcgatgaaat tagttgagga aactccgaga actttggtca
    10081 atgctacttt gtaggtagct tgtgtatatg gacttaaacc aatctctata caatattgta
    10141 agtattgatt gataagttct atccaacctt ccgtatcttt ttcaacatta cctaatgcca
    10201 ttaactgttc tggcgaaatg ttaattgaaa cccattttgc aaaacaatgc catgaacctg
    10261 aatagttatg gagtgtacga tgagtgtgga ttaactcaat gctttctttt ttccttgcag
    10321 tcggttctaa cgaaccgatg ttttgcgaac cgcgcaaaac ctgcttgcga agattgctcg
    10381 cataataaag ttgtaattat catttaaaat aatgttcaaa attacaacac attcttgttt
    10441 atttttgata taatgtaaaa aaacaacaaa ggattaaaca tgaataaacg agaacgacta
    10501 gaagaaatta cacgattggt aaacaaaaaa ggcacagtcc gaatttctga gattgttgaa
    10561 ttgctaaatg ttactgatat gactgttcga cgtgatttta ttgaactgga agagcaaggt
    10621 gtcttaacaa aaattcacgg cggtgctaga agtaataagg cttttcaata tcgggaatac
    10681 tcccattctg agaaacacat tcagaataag gaagccaagc aagagatagc ggaaagggct
    10741 gcacaactga tagaagatgg agacacggta tttttaggtc caggaacaac agtggccttt
    10801 ttggctgagg ccattaaaaa ttctcgccta acagtcatca caaactgtat gcctgttttt
    10861 accatcctat ctcaaaagaa gacagaggag tttcaagtct ttcttctggg gggagaatat
    10921 cgtcaagtga cagaatcttt tgtaggagaa attaccaata caagtttaga aaaaatgcga
    10981 ttcagcaaga ttttcttttc aggtaacgga cttaagggaa ctgatgtcat gacctctacc
    11041 ctagcggaag cctatacgca aaatattgcc attaaacatt ctttagaaaa atatctactg
    11101 ctagatgcct cgaaaattgg aaaagatgat tttacttcct tttgtgattt gcatgatgta
    11161 actgctctag tgaccgatat gaatgcaaag gatgaaaatt accaattatt aagtaagcac
    11221 atcgaaatta tcactacaaa attataaagt tatcaaaacc aacagaaaag tgttggtttt
    11281 ttgtttgtac acgaaaaaaa caaacaaaaa taaaacaaaa tatgttgact ttgtgtttgt
    11341 ttggttgtat aatttaaaca taaaaagcaa aaacgctttt tctaagtttt gcaaaacaaa
    11401 gattttctgc caaatatggc tacaactttc tatttttgtt aggagatagg ttatgacaat
    11461 cattatcggt gctgatccag cgggttcaaa actaaaagac gttattaaag attatttgct
    11521 agaaaaatca tatgacttta aagatgtcag tgatggaagt gattttgttg atacaacttt
    11581 ggcagtagtt gcagaagtta atcaacagga aggaaatctt ggcattgtga ttgatgctta
    11641 tggtgttggt ccatttatga ccgcaactaa agtcaaaggc atgattgctg cagaagtttc
    11701 agacgaacgt tctgcctata tgacaagagg acacaacaat tcgcgtatta ttacaatcgg
    11761 tagtgagatt gtaggagaag gaattgctaa aaatatcgtt aaaggatttg ttgaaggtga
    11821 gtatgatggt ggccgtcacc aaattcgtgt cgatatgcta aataaaatgt gctaattgtt
    11881 gtaaacaaag gagaataaga gatgaaaatt gcaatcggtt gtgaccacat tgtaacctat
    11941 gaaaaaatcg ctgtggtaga ttatttgaaa aatcaaggat atgaagtgtt ggactttggg
    12001 acttatgaca atgttcgtac tcattaccca atctttggta aaaaagttgg cgaagctgtt
    12061 gcaagcggac aagcagattt gggtatttgt atctgtggaa caggtgttgg aatcaacaat
    12121 gctgtaaaca aggttcctgg tgttcgatct gccctggttc gtgatatgac ctccgcgctt
    12181 tacgcgaagg aagagttaaa cgccaacgtc attggttttg gtgggaaaat tacaggtgag
    12241 ttgctcatgt gcgacatcat tgaagccttc attaaagcag aatacaaacc aactgaagca
    12301 aacaaacgtt tgattgaaaa gattgctgca gttgaaacgc tcaatcaaga gcaggctaat
    12361 ccagagttct ttactgaatt tcttgaaaag tgggatcgtg gagaatacca ggactaggag
    12421 gctcacatga ttttaacaat cactttaaat ccgtcagttg atattgccta tcaattggat
    12481 acctttcatt tggatacggt caatcgagta gaaaaagttc aaaaaacagc tggtggtaag
    12541 gggctcaatg ttacacgagt tttgaaacaa atcggtgaag atgttgtagc aacaggcttt
    12601 attggcggtg agattggaag ttatgtaaag aaacaactca ctcgaaatga tattaaaaat
    12661 tcctttgtgg aaattgggag cgaaacacgt aactgtattg cggttcttca tgatggcaaa
    12721 cagacggaga ttttagaaca aggaccaact attcaagaac atgaagcctt gaattttatc
    12781 gaacaccttg aaattattct gaacaatgta gatgttgttg ttatttcagg aagtttacct
    12841 aagggacttg ctagcaatta ttatgttgag attgtagaac tttgtaaaaa atgcggtgta
    12901 gctgttgttc tggattgttc tggtgaggcg ctgaaaaacg ttttagaaag ccagcagaag
    12961 ccgactgtga ttaagccgaa tacagaggag ttatcacaac tgattggtaa agaagttact
    13021 gatgatattc aagagttgaa atcagttttg tctggtcaac ttttccaagg aattgattgg
    13081 atagtcgtct cattaggagc acagggggct tttgccaagt acaaagacaa gttctatcgt
    13141 gtcagaattc caaaaatcaa agtcgtcaat ccagtaggat caggagattc aactgttgca
    13201 ggtattgcag caggtttggt acatgcttta cctgaagcag aattattaaa aaatgctaat
    13261 gttttgggca tgttaaatgc ccaggaagaa cagacaggtt atgttaattt agtacattca
    13321 gaatcattgt attctcaaat cgaagtcgag gaggtttaag atggtagtaa cagcagataa
    13381 aagaaaatat atggaaaaag tgagaaatga agcaggtatt atttcagctt tagcttttga
    13441 ccaacgtgga gccctgaaaa aaatgatggc aaaacatcag acagaagaac ctactgtgga
    13501 acagatggaa gaactaaaga gactagtttc ggaagaatta acacagtttt cttcctcaat
    13561 tctgttagat cctgaatttg gtttgccagc atcacgcgct cgtaacgagc agtgcggttt
    13621 gctcctagct tatgaaaaaa ctggctatga taccagctct actagtcgtt taccagattg
    13681 cctagtagag tggtctgtaa aacgactaaa agaagaaggg gcagatgcga ttaagtttct
    13741 actctactat gacgttgatg gtgatccata cgtcaatctt caaaaacagg cctatattga
    13801 acgcttgggt tcagaatgtc aggctgaagg tttaccattc ttcttggaaa ttttgagcta
    13861 tgatgaaacg attgaagaca atgctagtgt ggaatttgca aaagtcaaac ctcgaaaagt
    13921 aaatgaggcc atgagagtat tttcggatga acgctttgga gtggatgttc tcaaggtaga
    13981 agtacctgtg aacatgaatt ttgtagaagg ctttggaaca ggggaagttg tctatacaaa
    14041 agaagaagca gccgaatatt tccgtcagca agaagcgtca actaacttac catatattta
    14101 tctaagtgca ggtgtttccg ctaaactttt ccaagataca ctagtgtttg cacatgaagc
    14161 aggtgctaaa tttaacggtg ttctctgcgg tcgtgcgacg tgggcgggtg ttgtgccagt
    14221 ctatattgaa cagggtgaag aagctgcgcg tgaatggtta cgtaccgttg gtcgtgaaaa
    14281 tatcgaggga cttgatgcag tcttgtctca aacagcaact tcttggctag aaaaataatt
    14341 gaaaaaataa ccattttctc aaaagaaagg gtttacattt tctgagaata tgctataata
    14401 atctcatcag caagaataga gtggattgta actaattcga ttaggcaaga ctacaaatgg
    14461 atttgagagt aggatattct cggtttgttt gtagtctttt tttgcttaaa aatagaggag
    14521 ggatatatgt ttagaattat acagtcacta aataataacg ctgccctggt gaaaaatgaa
    14581 catggggaac aggcagtcgt gatgggatta ggtataacct ttcaaaagaa gaaaggggat
    14641 ttaattgtta aggacaagat agaaaatatc ttttcgctaa aaaatgatga agccaaagaa
    14701 aattttctaa ccttattaaa agatattccc ttagatttta tttcggcaac ctacacggtt
    14761 attaatcatc tggtagaaac ttatcattac cctgtacaag aatatctata tgtgacactg
    14821 acagatcata tctattgtgc atatcaggcg gttttaaaga atacctatca agaaagtagg
    14881 ttacctaata tttcagccga atatccattg gaatataaaa tggcgcgtga agccttagct
    14941 atgtttcgtg aaaagttgct aaatgatttt ccaaatgatg agattggtag aattgcactt
    15001 cacttaatca atgcaaaggg tgaaaccatt catcccacta gtgaagaaca tgacatcagt
    15061 aagaaaatta ttgctgaggt tgaacaaatt ttgatagaga atgggattaa acgttcaaaa
    15121 gaaaatagca atttttatga tcgctttatg attcatttgt cctattttct gaattactta
    15181 gatagatccc atcagtcaaa tgaatcgatt gctagtttgg aacagtacat caagctccag
    15241 aatccaagag cccatcaggt tggaagtcag atttttgaga tgattacatc attggttgat
    15301 gagccagtaa atgatagtga aaggttctat attgtactac atattcaacg gttattgtaa
    15361 atcaattcaa ttttaaaagg aggttcatat tatgaacaga gaagaaatta ctttattagg
    15421 ttttgagatt gttgcattcg ctggtgatgc tcgctctcgt ttgatggagg cactggctgc
    15481 tgctcaaaaa ggtgattacg caaaagcaga agaactagtt gaagctgcca atgcttgtat
    15541 tgttgaagcc caccatgctc agacaagttt gttgcagaaa gaagcggctg gtgaggattt
    15601 agcctttagt gtgacgctca tgcatggtca agaccatctc atgacgacat tattattgaa
    15661 agatatgatg ggtcatatga ttgaacttta caaacgaggt gataagtaat gaataaattg
    15721 attgaattca ttgagaaagg gaagcctttc tttgaaaaga tttcgagaaa cccttattta
    15781 agggctatcc gtgatggttt tatcgctgcc atgccggtta tcttgttctc aactctcttt
    15841 ctaatggtag catttgtacc taatatcttt ggatttactt ggtctgatga agcagttgcg
    15901 gctatcatga aaccttacgg ttatacaatg ggtattgtag ctgtcttagt tgcaggaacg
    15961 accgcaaaat ctttgacaga tgcgtttaac cgtcaattgc caaaaaccaa tcagattaac
    16021 ttcatttcaa ccatgattgc ctcaatttca ggtttcttat tactggcttc tgatggtatt
    16081 gaaggtggat ttgccaatgg ttacatggga actaagggac ttttgacagc ctttctggca
    16141 gccttcatta cggttaatat ctataaggtc tgtgtgaaaa acaatgttac cattcgtatg
    16201 ccagacgaag ttccaccgaa cgtatcccag gcatttaaaa atgtgattcc atatgcattg
    16261 tctatctttg tcttgtatgg aattgatctt gtgacacgtc aattccttgg aacaaacgtt
    16321 gctgaggcga tcctgaaatt atttgagcct ctatttacag ccgcagatgg ttatgttggt
    16381 attacaatta tctttggtgc ctatgctttg ttctggtttg tagggattca tggtccatct
    16441 attgtggaac cagcaattgc agccattact tatgccaata ttgaaaccaa cttccagctt
    16501 ctacaggcgg gtcaacatgc ggacaaaatc ctgacttcag gtactcaaat gtttatcgta
    16561 acaatgggtg gtacaggtgc taccttggtt gtgccattca tgtttatgtg gttgactaag
    16621 tctaaacgaa ataaagcaat tggacgtgct tcagttgttc caactttctt tggtgtaaat
    16681 gaaccgatct tgttcggtgc accacttgta ctcaacccag tattctttgt tccattcatc
    16741 ttagctccaa ttgctaacgt atggattttc aaattctttg ttgatacgct aaaaatgaac
    16801 agtttcagcg ttaacttgcc atggacaact ccaggaccat tggggattgt aatggggaca
    16861 aactttgctc cacttgcctt tgcactagct atcttattgg tagttgtcga tgtacttatc
    16921 tattatccat tcttgaaagt ttacgatgag cagattctcg ctgaagaaca atcagggaaa
    16981 gttgaaaata gtttgaaaga gaaagtggcg gctaacttta atactgccaa agcggatgct
    17041 attcttgaaa aagctgcagt cgaaaccgaa atttctgaac aaacaaatgt tttggtactt
    17101 tgtgcaggag gtgggacaag cggcttgctt gccaatgctt taacaaaagc tgcaaaagaa
    17161 tacggtgttc cagtcacagc aacagcagga agctatgggg cgcatcgtga gattttgcca
    17221 gagtatcaat tagtcattct agcaccacaa gtggcatcaa actacgatga tatgaaacaa
    17281 gaaaccgatg cattgggtat taaacttgcc aaaactgaag gggctcaata cattaaactc
    17341 actcgtgatg gtcaaggtgc tcttgacttt gtcaaacaac agttttagaa cctatattca
    17401 cagttcagca cttccatcta aggctagtct ttcagctact catcgttagt ttttttcaaa
    17461 atacagtcag tattccctca aaaaactgcc ttgattagct gaaaaactat ctcttatata
    17521 caagcacttc acttgtgaat acaggttctt aatcaaaagg ggagggagtt atcatctcca
    17581 actcctaccc ctttatttat ttttgataga agaaggtagg catatggtta aaacattacc
    17641 aaaagatttt atatttggtg gagcaactgc tgcatatcaa gcagagggtg ctacacagac
    17701 ggatggaaaa ggtcgtgttg catgggacaa gtatctagaa gataattact ggtacactgc
    17761 ggaaccggct tctgattttt ataatcgtta tccagttgat ttagaactaa gtgaacaatt
    17821 tggggtaaac ggcattcgta tttcaattgc gtggtctcgt attttcccaa caggctttgg
    17881 agcagtgaat ccaaagggag tagagtatta ccataaatta tttgcggaat gccataaacg
    17941 tcatgtagaa ccatttgtaa cgcttcacca ttttgataca ccagaaacgc tacattctaa
    18001 tggtgacttt ctaaatcgag aaaatattga gcacttcgtc aattacgcag cgttttgctt
    18061 caaagaattc cccgaagtta attactggac aacctttaat gaaatcggtc caattggtga
    18121 tgggcaatat ctagttggga agttcccacc agggattcag tatgacttag ctaaggtatt
    18181 ccaatctcac cacaatatga tggtttcaca tgccaaggca gttaagctct acaaggatgc
    18241 tggttatagt ggagaaattg gtgttgtaca cgcattgcca acgaaatatc cttatgatcc
    18301 aagcaaccct gacgatgtac gtgcagcgga actagaagat attattcata ataaatttat
    18361 cctagacgca acatacttgg gttactattc tgaaaaaacg cttgaaggcg ttcgacatat
    18421 tctgaaagta aatggtgggg aattagacct tcgtgatgaa gacttcgctg ccttagatgc
    18481 cgcaaaagat ttgaacgact tcttgggcat caattactat atgagtgatt ggatgagagc
    18541 tcatgatgga gaaactgaga ttatccataa tggtaaaggg gaaaagggaa gttctaagta
    18601 tcaaatcaaa ggagtcggtc gtagagagtc tccagttgat attccaaaaa cggactggga
    18661 ttggattatc tatccgcaag gcctttatga tcaaatcatg cgtgtaaaaa atgactatcc
    18721 aaactataag aaaatataca tcactgaaaa cggcctaggc tataaagatg agtttgtaga
    18781 tggtacagtc tatgatgatg gtcgaatcga ctacgtgaaa aagcatttag aggtgatttc
    18841 agatgcaatc tcagatggtg ccaatgttaa aggttatttc atctggtcct tgatggatgt
    18901 attttcttgg tcaaatggat acgagaaacg atatggtttg ttctatgttg actttgagac
    18961 ccaagaacgt tatccaaaga aaagtgcgta ttggtataaa aaagtagcgg aaacacaggt
    19021 tattgaataa aactaataca aggatgccta gctcaagtta gcatccttgt tttcatataa
    19081 gggaggcagt ttttatgctc gaattaaaaa atgagaactt aacagtacaa ttttctgaat
    19141 taggtgggca aattatttct attaaagaca aggatggtat cgagtatctt tggcaaggag
    19201 atccgaccta ttggagcggt caagcaccag ttctatttcc tatttgtgga agtttacgaa
    19261 atgattgggc tatctatgaa cctgcagaaa gaccgacatt tacaggcaca attccaaggc
    19321 atggacttgt gagaaaaatg ctgttcaaaa atgtaaaaaa tactgaaaac tctttagaat
    19381 tttctatttc atcaaacgaa gaaaccgtaa aaaattaccc ttttgaattt gaactttcta
    19441 ttaactactc actatttggc aaaacaattc gaactgaata tcggatgaaa aaccttgagg
    19501 gacatagaaa gctaccttac tttattgggg gtcacccagg ttttaattgt cccttgttgg
    19561 atggtgagag ttatgaagac tattatttag aatttgaaaa agtagaatct tgtacagtac
    19621 ctagaagttt tccagaaact ggcttacttg atctgcgaga tcgccggcca ttcttggaaa
    19681 atcagaagac cttggacttg tcctataaac tgtttgagca tgatgccata acattggatc
    19741 aattagcatc acgaaaggta accttgaaat caaaaaatca tcagaagaaa ctttcgattg
    19801 catttgatga ttttccatat ttagtgattt ggtcaactac aaatcagagt ccctttatcg
    19861 cattagaacc atggagcgga ctctctacat cactggaaga atctgacata ttcaatgcca
    19921 aacgaaatat tagttacgtt gctccaaaag aa
//
LOCUS       DBNFEG010000016        19164 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.016, whole genome shotgun sequence.
ACCESSION   DBNFEG010000016 DBNFEG010000000
VERSION     DBNFEG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19164
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.016"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            12..410
                     /gene="spx"
                     /locus_tag="AC1PNG_000405"
     CDS             12..410
                     /gene="spx"
                     /locus_tag="AC1PNG_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HIP6700942.1"
                     /translation="MIKIYTVSSCTSCKKAKNWLNAHQLSYNEHNLAKEAITKEEILN
                     ILTKTENGIASIVSSKNRYAKSLDFDIEELSVNEVIDLITSNPRILKSPILIDEKRLQ
                     VGYKEDDIRAFLPRAVRNVENAQARMRAAL"
     gene            510..776
                     /locus_tag="AC1PNG_000406"
     CDS             510..776
                     /locus_tag="AC1PNG_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HIP6700943.1"
                     /translation="MGFTEETVRFRLDDTDKQEISKTLTSVYRSLEEKGYNPINQIIG
                     YVLSGDPAYIPRYNDARNQIRKHERDEIIEELVRYYLKGNGIDL"
     gene            776..1195
                     /gene="ruvX"
                     /locus_tag="AC1PNG_000407"
     CDS             776..1195
                     /gene="ruvX"
                     /locus_tag="AC1PNG_000407"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIP6700944.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIPIDEEKGEFGLER
                     LTELVEQYKVDKFVVGLPKNMNNTSGPRVEASQAYGDLLTERYKLPVDYQDERLTTVA
                     AERMLIEQADISRGKRKKVIDKLAAQLILQNYLDRTF"
     gene            1207..1527
                     /locus_tag="AC1PNG_000408"
     CDS             1207..1527
                     /locus_tag="AC1PNG_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HIP6700945.1"
                     /translation="MAHHHDHEHDHNHDERELITLVDDQGNETLFEILLTIDGQEEFG
                     KNYVLLIPASAEEDENGEVEIQAYSYIENENGTEGDLQPIPEDATAEWDMIEEVFNSF
                     MEEE"
     gene            1785..2363
                     /locus_tag="AC1PNG_000409"
     CDS             1785..2363
                     /locus_tag="AC1PNG_000409"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIP6700946.1"
                     /translation="MKDSFTERSKRLSEELERCLLADKNILVILDIMDRLNLSDCWLC
                     AGTIRNFIWNQYSFDEETDVDLVFFDENISYEETMEIESDLYQNYPAYRWELKNQVYM
                     HIHSPNTKPNQSSRDAIEKFPERCTAIGICRTERNKLEIFAPFGLDDIYAYQVRPTPH
                     FLADPNRMELYRQRILKKQWKKRWLSLTVKET"
     gene            2436..2702
                     /locus_tag="AC1PNG_000410"
     CDS             2436..2702
                     /locus_tag="AC1PNG_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIP6700947.1"
                     /translation="MEFETVCLKVRNLGRLYCYFKVKFRNHIEAIIRKQESQKCKFDR
                     LFHEDIHSLIQSPSLGNDELLMLWGLLRDYRKNLINDRLENMKN"
     gene            3024..3332
                     /gene="rpsJ"
                     /locus_tag="AC1PNG_000411"
     CDS             3024..3332
                     /gene="rpsJ"
                     /locus_tag="AC1PNG_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIP6700948.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVETATRTGAQVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIINPTQKTVDALMKLALPSGVNVEIKL
                     "
     gene            3545..4171
                     /gene="rplC"
                     /locus_tag="AC1PNG_000412"
     CDS             3545..4171
                     /gene="rplC"
                     /locus_tag="AC1PNG_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIP6700949.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYAAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGQEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGNRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            4196..4819
                     /gene="rplD"
                     /locus_tag="AC1PNG_000413"
     CDS             4196..4819
                     /gene="rplD"
                     /locus_tag="AC1PNG_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIP6700950.1"
                     /translation="MANVTLFDQTGKQAGEVVLNDAIFGIEPNQAVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYAYKLP
                     QKVRRLALKSVYSEKVAENKFVAVNSLEFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNIPGVKVATATTASVLDIANADKLLVTQAAISKIEEVLA"
     gene            4819..5118
                     /locus_tag="AC1PNG_000414"
     CDS             4819..5118
                     /locus_tag="AC1PNG_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIP6700951.1"
                     /translation="MNLYDVIKKPVITESSMGQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKTKRVGRYVGRTNKVKKAIITLAADSKAIELFATADAE"
     gene            5136..5969
                     /gene="rplB"
                     /locus_tag="AC1PNG_000415"
     CDS             5136..5969
                     /gene="rplB"
                     /locus_tag="AC1PNG_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIP6700952.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDGVEAIVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTVVHNIELKPGRGGELVRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATVGTVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNQ
                     K"
     gene            6071..6352
                     /gene="rpsS"
                     /locus_tag="AC1PNG_000416"
     CDS             6071..6352
                     /gene="rpsS"
                     /locus_tag="AC1PNG_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIP6700953.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            6370..6714
                     /gene="rplV"
                     /locus_tag="AC1PNG_000417"
     CDS             6370..6714
                     /gene="rplV"
                     /locus_tag="AC1PNG_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIP6700954.1"
                     /translation="MAEITSAKATARTVRVSPRKSRLVLDNIRGKSVADAIAILKFTP
                     NKAAGIIEGVLNSAIANAENNFGLEKANLVVSEAFANEGPTLKRFRPRAKGSASPINK
                     RTAHITVVVAEK"
     gene            6727..7380
                     /gene="rpsC"
                     /locus_tag="AC1PNG_000418"
     CDS             6727..7380
                     /gene="rpsC"
                     /locus_tag="AC1PNG_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIP6700955.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRNFIKK
                     ELADASTSTIEIERAVNKVIVSIHTAKPGMVIGKAGSNVDALRAQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGESIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEALTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            7384..7797
                     /gene="rplP"
                     /locus_tag="AC1PNG_000419"
     CDS             7384..7797
                     /gene="rplP"
                     /locus_tag="AC1PNG_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIP6700956.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKQVDFGQYGLQATTSSWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEVAGVSEEIAREAFRLAGHKLPVKVKFVKREAE"
     gene            7807..8013
                     /gene="rpmC"
                     /locus_tag="AC1PNG_000420"
     CDS             7807..8013
                     /gene="rpmC"
                     /locus_tag="AC1PNG_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIP6700957.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFELRFQAAAGQL
                     EQTARLNEVKKQIARIKTVQSETK"
     gene            8037..8297
                     /gene="rpsQ"
                     /locus_tag="AC1PNG_000421"
     CDS             8037..8297
                     /gene="rpsQ"
                     /locus_tag="AC1PNG_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIP6700958.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            8322..8690
                     /gene="rplN"
                     /locus_tag="AC1PNG_000422"
     CDS             8322..8690
                     /gene="rplN"
                     /locus_tag="AC1PNG_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIP6700959.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDIIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRADGSYIKFDENAAVIIREDKNPRGTRIFGPV
                     ARELRDGGFMKIVSLAPEVL"
     gene            8829..9134
                     /gene="rplX"
                     /locus_tag="AC1PNG_000423"
     CDS             8829..9134
                     /gene="rplX"
                     /locus_tag="AC1PNG_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIP6700960.1"
                     /translation="MFVKKGDKVRVIAGKDKGVEAVVVTALPKVNKVIVEGVNIVKKH
                     QKPNSENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            9158..9700
                     /gene="rplE"
                     /locus_tag="AC1PNG_000424"
     CDS             9158..9700
                     /gene="rplE"
                     /locus_tag="AC1PNG_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIP6700961.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGDA
                     VSNAKNLEKAAQELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVTVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGMDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            9715..9900
                     /locus_tag="AC1PNG_000425"
     CDS             9715..9900
                     /locus_tag="AC1PNG_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIP6700962.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCEKCGRPHSVYRKFKLCRVCF
                     RDLAYLGQIPGVTKASW"
     gene            10225..10623
                     /gene="rpsH"
                     /locus_tag="AC1PNG_000426"
     CDS             10225..10623
                     /gene="rpsH"
                     /locus_tag="AC1PNG_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIP6700963.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIATILKNEG
                     FVKNVEFIEDDKQGIIRVFLKYGPNGEKVITNLKRVSKPGLRVYSKREDIPKVLNGLG
                     IAIISTSEGLLTDKQARQKNVGGEVIAYVW"
     gene            11088..11624
                     /gene="rplF"
                     /locus_tag="AC1PNG_000427"
     CDS             11088..11624
                     /gene="rplF"
                     /locus_tag="AC1PNG_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIP6700964.1"
                     /translation="MSRIGNKVITLPAGVELAQNNGVVTVKGPKGELTREFPTAIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTSRANLNNMVVGVSEGFKKELEMRGVGYRAQLAGNK
                     LTLAVGKSHPDEVVAPEGITFEVPTPTQIVVSGINKEVVGQTAAYIRSLRAPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            11713..12069
                     /gene="rplR"
                     /locus_tag="AC1PNG_000428"
     CDS             11713..12069
                     /gene="rplR"
                     /locus_tag="AC1PNG_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIP6700965.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKISGTAARPRLNIFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALAESARENGLKF"
     gene            12088..12582
                     /gene="rpsE"
                     /locus_tag="AC1PNG_000429"
     CDS             12088..12582
                     /gene="rpsE"
                     /locus_tag="AC1PNG_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIP6700966.1"
                     /translation="MAFKDNAVEIEERVVAINRVTKVVKGGRRLRFAALVVVGDRNGR
                     VGFGTGKAQEVPEAIRKAVESAKKNMIEVPMVGTTIPHEVRSEFGGARVLLKPASEGS
                     GVAAGGATRAVIELAGIADVTSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            12597..12779
                     /gene="rpmD"
                     /locus_tag="AC1PNG_000430"
     CDS             12597..12779
                     /gene="rpmD"
                     /locus_tag="AC1PNG_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIP6700967.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNPAIL
                     GMVNAISHLVTVEEVK"
     gene            12915..13355
                     /gene="rplO"
                     /locus_tag="AC1PNG_000431"
     CDS             12915..13355
                     /gene="rplO"
                     /locus_tag="AC1PNG_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIP6700968.1"
                     /translation="MKLHELQPATGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRPGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNAFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITSKGGSVEVI"
     gene            13369..14679
                     /gene="secY"
                     /locus_tag="AC1PNG_000432"
     CDS             13369..14679
                     /gene="secY"
                     /locus_tag="AC1PNG_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIP6700969.1"
                     /translation="MFFKLLKDALKVKLVRSKILFTIFILFVFRVGTHITVPGVNAKS
                     LEALSNLPFLNMLSLVSGNAMRNFSVFALGVSPYITASIIVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLVLAFVQSIGITAGFNALSGAKLTNMPLNWQTYLLIGAILTT
                     GSIIVTWLGEQISEKGYGNGTSMIIFAGIISSLPGTFHEIYIDRFVNIESSRLGESAI
                     FVAALVVLIFFVVYFTTFVQQAEYKLPIQYTKRAQGAPSSSYLPLKLNPAGVIPVIFA
                     GSITAVPTSLIQYFASQNKSAGWLLTVQEYFDYSTAKGMIVYAGLIIAFTFFYTFVQV
                     NPEKTAESLQKSAAYIHGVRPGNGTEQFLSKLLTRLAVIGALFLSFVALLPILAQNLF
                     GLSSSIAFLGTSLIIVISTSIEGIKQLEGYLLKRKYVGFLEITE"
     gene            14773..15414
                     /locus_tag="AC1PNG_000433"
     CDS             14773..15414
                     /locus_tag="AC1PNG_000433"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIP6700970.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEKFNVAHISTGDMFRAAMANQTE
                     MGILAKSYIDKGDLVPDEVTNGIVKERLVQDDIKEKGFLLDGYPRTIEQAHALDENLA
                     DLGIELQGVINIEIDPSKLVERLSGRIIHKETGETFHKVFNPPVGDYKEEDFYQREDD
                     KPESVKRRLEVNIAQGQPIIDHYRAKGLVHDIEGDQDIDLVFQAIDTVLSKLQ"
     gene            15536..15754
                     /gene="infA"
                     /locus_tag="AC1PNG_000434"
     CDS             15536..15754
                     /gene="infA"
                     /locus_tag="AC1PNG_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIP6700971.1"
                     /translation="MAKEDVIEIEGKVVDTMPNAMFTVELENGHQVLATVSGKIRKNY
                     IRILVGDRVTVELSPYDLTRGRITYRFK"
     gene            15779..15895
                     /gene="rpmJ"
                     /locus_tag="AC1PNG_000435"
     CDS             15779..15895
                     /gene="rpmJ"
                     /locus_tag="AC1PNG_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIP6700972.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            15915..16280
                     /gene="rpsM"
                     /locus_tag="AC1PNG_000436"
     CDS             15915..16280
                     /gene="rpsM"
                     /locus_tag="AC1PNG_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIP6700973.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VKDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            16298..16681
                     /gene="rpsK"
                     /locus_tag="AC1PNG_000437"
     CDS             16298..16681
                     /gene="rpsK"
                     /locus_tag="AC1PNG_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIP6700974.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            16726..17664
                     /locus_tag="AC1PNG_000438"
     CDS             16726..17664
                     /locus_tag="AC1PNG_000438"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIP6700975.1"
                     /translation="MIEFEKPTITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSIKIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKKIELDVVGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIADGATFKAVLTVNSGRGYVPAEDNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTLEINTNGTIVPEDALGLSARILMEH
                     LGLFTDLTEVAKSAEVMKEAEVASDDRMLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKTEPEMMKVRNLGRKSLEEVKVKLADLGLGLKKDK"
     gene            17679..18065
                     /gene="rplQ"
                     /locus_tag="AC1PNG_000439"
     CDS             17679..18065
                     /gene="rplQ"
                     /locus_tag="AC1PNG_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIP6700976.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            18376..19164
                     /locus_tag="AC1PNG_000440"
                     /pseudo
     CDS             18376..19164
                     /locus_tag="AC1PNG_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987985.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
BASE COUNT         6145 a         3618 c         4133 g         5268 t
ORIGIN      
        1 ggagtgacta catgattaaa atttatactg tatctagttg tacttcttgt aagaaagcga
       61 aaaattggtt aaatgctcac caattatctt ataatgagca taatcttgcg aaggaagcca
      121 ttactaagga agaaatccta aacatcttaa caaaaacaga gaatggtatt gcaagtatcg
      181 tatcgtcaaa aaaccgttat gcaaagagtc tggactttga tattgaagag ttgagtgtga
      241 acgaggtcat tgatttaatt acctctaacc cacgtatttt gaagagtcca atcttgattg
      301 atgagaaacg acttcaagtg ggctataaag aagatgatat acgcgctttc ttgccacgtg
      361 cagtgcgcaa tgttgaaaat gctcaagctc gtatgagagc agctctataa gatgtacaaa
      421 aggactcttt tggggttctt tttgcgcatt ttttgagaat aattaaaatt tgtgatataa
      481 tgtaaaaaat agggaaagaa ggtgtaagta tgggatttac cgaagaaacc gttcgttttc
      541 gcctagatga tacagataaa caagaaatca gcaagacatt gactagtgtt tatcgttcgc
      601 tagaggaaaa gggctataat ccgattaacc aaatcattgg ctatgtatta agtggggacc
      661 ctgcttatat tcctcgctat aatgatgccc gcaatcagat tcgtaagcat gaacgagatg
      721 aaatcattga agaattggtg cgctactatt tgaaagggaa tgggattgac ctctaatgag
      781 aataatggga ttagacgtcg gttccaagac agttggtgta gccatttcag atccgttagg
      841 tttcacggcc caagggttgg aaatcatccc aatcgatgaa gaaaagggcg aattcggtct
      901 ggagcgtttg accgaacttg tagaacagta caaggttgat aaatttgttg taggcttgcc
      961 gaagaatatg aataatacta gtggtccacg tgtggaagct agtcaagctt acggtgattt
     1021 attgactgag cgttacaaac ttccagttga ttatcaggat gaacgtttaa caactgttgc
     1081 agcagaacgg atgctgattg agcaagcgga tatcagccga ggaaaacgca agaaagttat
     1141 cgataaatta gcagctcagc tgattttaca aaattattta gatagaacat tttaaaggag
     1201 attctaatgg cacatcatca tgaccatgaa cacgaccaca accacgacga acgtgaattg
     1261 attactttgg tagacgacca aggtaacgaa acactttttg aaatcttatt gacgattgac
     1321 ggtcaagaag agtttggtaa gaactatgtt ctcttgattc ctgcaagtgc agaagaagat
     1381 gagaacggag aagttgaaat ccaagcctac tcatacattg agaatgagaa tggcacagaa
     1441 ggcgatttgc aacctatccc agaagacgca acagcagaat gggatatgat tgaagaagtc
     1501 ttcaacagct ttatggaaga ggaataaaaa ggtccagtgg acctttttat catgagcatg
     1561 aaaacaagaa agcgaattaa ggtccggtgg acctttttat catgagcctg aaaacaagaa
     1621 agtgaattaa ggtccagtgg acctttttgt catgagcctg aaaacaagaa agcgaattaa
     1681 ggtccggtgg agtgaagcag tctgggggag actgtttcag cccgagttaa gaaacagcaa
     1741 ggcgaggaat ggattatgag ccagaaaatc aaaagggagg ccagatgaaa gatagtttta
     1801 cagaaagaag taagaggctg agtgaggaat tagagaggtg tctactggct gataaaaata
     1861 ttttggtcat attagacata atggatagac tgaatttatc agattgctgg ctctgtgcgg
     1921 gaactatccg taatttcatt tggaatcaat acagttttga tgaagaaaca gatgttgatc
     1981 ttgtgttttt tgatgaaaat atttcttatg aagagacaat ggaaatagaa agcgatttat
     2041 atcaaaacta tccagcctat cgatgggaat taaaaaatca agtgtatatg catatccata
     2101 gtccgaatac taaaccgaat caatcatcaa gggatgcgat tgaaaaattt ccagaaagat
     2161 gcacagcgat aggtatttgt cgaacagaaa gaaataaatt agaaatattt gctccttttg
     2221 gattggatga catttatgct taccaagtac ggccaacacc acatttctta gcggatccta
     2281 atcgcatgga attgtatcga caaagaattc ttaagaaaca gtggaaaaag aggtggctat
     2341 cgctaacagt aaaagagact taactcccac caagtaagta ggccaaaaac agtatttttt
     2401 ataaactaaa acaatcaact acgggagatt atgttatgga atttgaaacg gtttgtttga
     2461 aggttagaaa cttaggtagg ctatattgct acttcaaagt taaatttaga aaccatatcg
     2521 aggctataat tcgtaaacag gaaagtcaaa aatgtaaatt tgatcgtctc ttccatgaag
     2581 atattcatag tttgattcaa tcaccgagct tgggtaatga tgagttgctg atgttatggg
     2641 gtctcctgag agattatcga aaaaatttaa tcaatgaccg acttgaaaat atgaaaaatt
     2701 gataagcgga cagagtttta ctggcagtca aaaaaataat tcgagactta caaggatact
     2761 tgaaaaaaac gttgatagtt aactcaaata aaaaataagt aatgaagaat cctgaaataa
     2821 aagttttggg attttttgaa tttttgagta aaaactcttg caatggcaag tggaatctgg
     2881 tagaatgttc tagtgccgta aaaaatacgg ctgtagcttt gatgcaagag gttgcgacac
     2941 gctcggttgc attgccacgc aacacgtgtt ggttttcttg tggagctagc ctattatgtt
     3001 aaatagacga aaaggagaaa aagatggcaa acaaaaaaat tcgcatccgc ttgaaagcgt
     3061 acgaacaccg tacacttgat acagcagctg caaaaatcgt tgaaactgca acacgtacag
     3121 gtgctcaagt agcaggtcca gttccgcttc caacagaacg tagcctctac acaatcatcc
     3181 gtgcgactca caagtataaa gactctcgtg agcagttcga aatgcgtaca cacaaacgtt
     3241 tgatcgacat cattaaccca actcaaaaaa cagttgatgc tttgatgaaa ttggctcttc
     3301 caagtggtgt aaacgtagaa atcaaattgt aatcaagtag caaggtaaac taaaagcaag
     3361 tgttcataat tgaacacggg ctaaactccg tgtgaaaaag ataaactttc ctagaaacag
     3421 aagtttcttc gtcaagtttc ctattttcac tttgagtttg acgccctttg tatcttggtt
     3481 tgagcatgaa aaacgctcgt taaaaacttt ttaactataa aataagaaaa ggaatatttt
     3541 tctcatgaca aaaggaatct tagggaaaaa agtgggaatg actcaaatct tcactgaatc
     3601 tggtgaattt atccctgtta ctgtcatcga agcaactcca aacgttgttc ttcaagtgaa
     3661 aacagttgaa actgacggtt atgcagcagt tcaagttggt tttgatgaca aacgtgaagt
     3721 attgagcaac aaacctgcca aaggccatgt agctaaagct aacacagctc ctaagcgctt
     3781 cattcgtgaa ttcaaaaaca ttgaaggctt ggaagttgga caagaaatta cagttgaaac
     3841 tttcgcagct ggtgatgttg ttgatgtaac tggtacatct aaaggtaaag gtttccaagg
     3901 cgttatcaaa cgccatggtc aatcacgtgg tcctatggct cacggttctc gttaccaccg
     3961 tcgtccaggt tctatgggtc ctgttgcacc taaccgtgta ttcaaaggta aaaaccttgc
     4021 aggtcgcatg ggcggcaacc gtgtaacaat tcaaaacctt gaagttgtac aagttgttcc
     4081 agaaaagaac gttatcctta tcaaaggtaa cgtaccaggt gctaagaaat ctcttatcac
     4141 tatcaagtca gcagttaaag ctggtaaata ataaggaaag gggaaatcag tcgaaatggc
     4201 aaacgtaaca ttatttgacc aaactggtaa acaagctggt gaagtagttc ttaacgatgc
     4261 gatctttggt atcgagccaa accaagcagt tgtatttgat gtgatcatca gccaacgtgc
     4321 tagccttcgt caaggtactc acgcagttaa aaaccgttca gcagtctcag gtggcggacg
     4381 caaaccatgg cgtcaaaaag gaactggacg tgctcgtcaa ggttctatcc gttcaccaca
     4441 atggcgtggc ggtggcgtag tcttcggacc aactccacgt tcatacgcgt acaaacttcc
     4501 acaaaaagtt cgtcgcttgg cacttaaatc tgtttactca gaaaaagttg ctgaaaacaa
     4561 atttgtagct gttaactcgc ttgaattcac agctccaaaa actgctgaat ttgcaaaagt
     4621 acttgcagca ttgagcattg attctaaagt ccttgttatt cttgaagaag gcaacgaatt
     4681 cgcagctctt tctgctcgta acatcccagg agttaaagtt gcaactgcaa caactgcaag
     4741 cgtacttgac atcgcaaatg cagacaaact tcttgtaact caagcagcta tctctaaaat
     4801 tgaggaggtt cttgcataat gaatttgtat gatgttatca aaaaacctgt catcacagaa
     4861 agctcaatgg gccaactcga agcaggcaag tatgtatttg aagttgacac tcgtgcgcac
     4921 aaactcttga tcaagcaagc tgttgaagct gcattcgagg gtgttaaagt tgcaaatgtt
     4981 aacacaatca acgtgaaacc taaaacaaaa cgcgtaggtc gttatgtagg tcgcacaaac
     5041 aaagtgaaaa aagcaatcat cacattggct gctgattcaa aagcgatcga attgttcgct
     5101 acagctgacg ctgaataatc aaaggaggaa ttaacgtggg tattaaagtt tataaaccaa
     5161 cgacaaatgg ccgtcgtaac atgacttctt tggacttcgc tgaaatcaca acaagcactc
     5221 cagaaaaaag cttgcttgtt gctttgaaga gcaaagctgg tcgtaacaat aacggtcgca
     5281 tcactgttcg tcaccaaggt ggcggtcaca aacgtttcta ccgtttggta gacttcaaac
     5341 gtaacaaaga tggcgttgaa gcaatcgtta aaactatcga atacgatcca aaccgttcag
     5401 caaacatcgc tcttgtacac tacacagacg gtgttaaagc ttacatcatt gctcctaaag
     5461 gtcttgaagt tggtcaacgc atcgtttcag gtccagaagc agatatcaaa gttggtaacg
     5521 cacttccact tgcaaacatc ccagtcggta ctgttgttca caacatcgag ttgaaaccag
     5581 gtcgcggtgg tgagttggtt cgtgctgctg gtgcatctgc acaggttctt ggtcaagaag
     5641 gtaaatacgt tcttgttcgc cttcaatcag gtgaagttcg tatgatcctt ggtacttgcc
     5701 gtgctactgt tggtactgta ggtaacgaac aacatggcct tgttaacctt ggtaaagcag
     5761 gtcgtagccg ttggaaaggt atccgtccaa cagttcgcgg ttctgtaatg aaccctaacg
     5821 atcacccaca cggtggtggt gaaggtaaag caccagttgg tcgtaaagca ccatctacac
     5881 catggggtaa accagctctt ggtttgaaaa ctcgtaacaa gaaagctaaa tctgacaaac
     5941 ttatcgttcg tcgtcgcaac caaaaataat ctattcttgg ttgcttagca ttctatatca
     6001 tccgccaact cggtagcccg caaagcgggc aagccgctgt ggtacatatt ttaaaggaga
     6061 aaacactaaa atgggacgta gtcttaaaaa aggacctttc gtcgatgagc atttgatgaa
     6121 aaaagttgaa gctcaagcaa acgacgaaaa gaaaaaagta attaaaactt ggtcacgtcg
     6181 ttcaacgatt ttcccaagtt tcatcggtta tacaatcgca gtttacgatg gacgtaaaca
     6241 cgtacctgta tacattcaag aagacatggt aggtcacaaa cttggtgaat ttgcaccaac
     6301 tcgtacttac aaaggtcatg ctgctgacga caagaaaact cgtcgtaaat aatttaggag
     6361 gaaaacacaa tggcagaaat tacttcagct aaagcaactg ctcgcacagt acgtgtttca
     6421 cctcgtaaat cacgtcttgt cttggataac atccgtggca aaagcgtagc agacgcaatc
     6481 gcaatcttga aattcacacc aaacaaagct gcaggcatta tcgagggagt tttaaactca
     6541 gcaatcgcta acgctgaaaa caactttggt ttggaaaaag ctaacttggt agtcagcgaa
     6601 gcattcgcaa acgaaggacc aacgttgaaa cgtttccgtc cacgtgcgaa aggttcagct
     6661 tcaccaatta acaaacgcac agctcacatc actgtagttg tggcagagaa ataaggaggt
     6721 aaaaacgtgg gtcaaaaagt acatccaatt ggtatgcgtg ttggcatcat ccgtgattgg
     6781 gatgctaaat ggtatgctga aaaagaatac gcggattacc ttcatgaaga tcttgcaatc
     6841 cgcaacttta tcaaaaaaga attggctgat gcgtcaacat caacaatcga aatcgaacgt
     6901 gctgtaaata aagtaatcgt ttctatccac actgctaagc caggtatggt tatcggtaaa
     6961 gctggtagca acgttgatgc acttcgtgct caattgaaca aattgactgg taagcaagta
     7021 cacatcaaca tcatcgaaat caaacaacct gatttggatg cacaccttgt tggtgagtca
     7081 atcgctcgtc aattggagca acgtgtggca ttccgccgtg ctcaaaaaca ggctatccaa
     7141 cgtgcaatgc gcgctggtgc aaaaggtatc aaaacacaag tttctggtcg tttgaacggt
     7201 gctgatattg cccgtgcaga aggctactca gaaggaactg ttcctcttca tacacttcgt
     7261 gcggatatcg actacgcttg ggaagaagct ttgacaactt acggtaaact tggtgttaaa
     7321 gtatggatct accgtggtga agttcttcca gctcgtaaaa acactaaagg aggtaaataa
     7381 caaatgttag tacctaaacg tgtaaaacac cgtcgtgaat tccgtggaaa aatgcgcggt
     7441 gaagctaaag gtggaaaaca agtagacttt ggtcaatacg gtcttcaagc aactactagc
     7501 tcatggatta caaaccgcca aatcgaagct gcccgtatcg ctatgacgcg ttacatgaaa
     7561 cgtggtggta aagtttggat caagatcttc ccacacaaat catacactgc taaagctatc
     7621 ggtgtacgta tgggttctgg taaaggtgct cctgaaggtt gggtagctcc agttaaacgc
     7681 ggtaaggtta tgtttgaagt agctggcgtt tctgaagaaa tcgctcgcga agcattccgc
     7741 cttgctggtc acaaattgcc agttaaagtt aaattcgtaa aacgtgaagc agaataagga
     7801 gaagacatga aacttcaaga aattaaagat tttgtaaaag aacttcgtgg cctttctcaa
     7861 gaagaacttg ctaagaaaga aaacgaattg aagaaagaac tcttcgaact tcgtttccaa
     7921 gctgctgctg gtcaacttga gcaaactgct cgtttgaacg aagtgaagaa acaaattgca
     7981 cgtatcaaaa ctgtgcaatc tgaaactaaa taatagattg ggaaaggaga atttcaatgg
     8041 aacgcaataa tcgtaaagtt cttgttggac gcgtagtatc tgacaaaatg gacaaaacaa
     8101 tcacagttgt agttgaaact aaacgtaacc acccagtcta tggtaaacgt attaactact
     8161 ctaaaaagta caaagctcat gatgaaaaca atgttgctaa agaaggcgat atcgttcgta
     8221 tcatggaaac tcgcccactt tcagctacaa aacgtttccg tcttgtagaa gttgtggaag
     8281 aggcagttat catttaatca acctgaaagg agaaaattga catgattcaa acagaaactc
     8341 gtttgaaagt tgctgacaac agtggcgcac gtgaaatctt gacaatcaaa gttcttggtg
     8401 gttcaggacg taaattcgcg aacatcggcg acatcatcgt tgcttcagta aaacaagcta
     8461 ctcctggtgg tgcggttaaa aaaggtgacg ttgttaaagc cgttatcgtt cgtactaaga
     8521 caggtgctcg tcgtgctgat ggttcataca tcaaattcga tgagaatgct gcagttatca
     8581 tccgtgaaga caaaaaccct cgcggaactc gtatctttgg cccagtggca cgcgaattgc
     8641 gtgatggcgg tttcatgaaa atcgtttcat tggcaccaga agtactttaa tcaaatagtt
     8701 ttaggaaatt tgttacaaac taacgaagtc tgtgacgggt ttagtaaact agaaaacaaa
     8761 ctaagtcccc tggttaaatc actaaccagg gtgcccatta gggcgtaaga aattatagga
     8821 gaaatcaaat gtttgtaaaa aaaggcgata aagttcgcgt aatcgctggt aaagacaaag
     8881 gcgttgaagc agttgtcgta acagcacttc caaaagtaaa caaagttatt gttgaaggtg
     8941 ttaacatcgt taagaaacac caaaaaccaa acagcgaaaa ccctcaaggc gctatcgttg
     9001 aaaaagaagc tccaatccat gtgtcaaacg ttcaagttct tgacaaaaat ggtgttgcag
     9061 gacgcgttgg ttacaagttt gtagacggca aaaaagttcg ctacaacaaa aaatcaggcg
     9121 aagtacttga ttaatcacga aggaaaggag aagtataatg gcaaatcgtt taaaagaaaa
     9181 atatcttaat gaagtagttc ctgctttgac tgaacaattt aactattctt cagttatggc
     9241 tgtgccaaaa gttgataaga tcgttttgaa catgggtgtt ggtgacgctg tttctaacgc
     9301 taaaaacctt gagaaagctg ctcaagaatt ggctttgatc tcaggtcaaa aaccacttat
     9361 cactaaagct aagaaatcaa tcgccggctt ccgtcttcgt gagggtgttg cgatcggtgc
     9421 gaaagtaact cttcgtggcg aacgtatgta tgaattcttg gacaaattgg ttacagtttc
     9481 acttccacgt gtacgtgact tccacggtgt accaactaag tcatttgacg gacgtggtaa
     9541 ctacacactt ggtgtgaaag agcaattgat cttcccagaa atcaacttcg acgatgttga
     9601 taaaactcgc ggtatggata tcgttatcgt tacaactgct aacactgacg aagaatcacg
     9661 tgcattgctt actggccttg gtatgccgtt tgcaaaataa gagggagaga ataaatggct
     9721 aaaaaatcaa tgatcgctaa gaacaaacgc ccagctaagt tctctacaca agcttacaca
     9781 cgctgtgaaa aatgtggacg tccacactca gtttaccgca aattcaaatt gtgccgtgta
     9841 tgcttccgcg acttggctta ccttggacaa attccaggcg taacaaaagc ttcttggtaa
     9901 gataagatgt gagagcgttt agcaaccaac gcaaagatag gagattttac aacgaagttt
     9961 acttctagga tttgctaagc ttgtagctcg aactcaatta aaacatgata ctaagggcgt
    10021 gacggacaaa gtgaaaatag gaactctagc gaagaacgct tgcgttctag atagagttat
    10081 ctttttcaca caggccgtag cccgagttca aatgttagga tgcaatagca tcatactagc
    10141 aaattcaaac tttgcattgc ctatcaggca cacattaact agtaagtgat ctaatcaaac
    10201 tgctagtaag aggagaaaca acaaatggtt atgactgacc caattgcaga ttttttgaca
    10261 cgtattcgta acgctaacca agcaaaacac gaagtgcttg aagtacctgc atcaaacatc
    10321 aaaaaaggta ttgctacaat ccttaaaaac gaaggttttg taaaaaacgt tgaattcatc
    10381 gaagatgaca aacaaggcat catccgtgta ttcttgaaat acggaccaaa cggtgaaaaa
    10441 gttatcacaa acttgaaacg cgtttcaaaa ccaggtcttc gtgtttactc aaaacgtgaa
    10501 gatattccta aagttcttaa cggacttggt atcgcaatca tctcaacatc agaaggtctt
    10561 ttgactgaca aacaagctcg tcaaaagaac gttggtggtg aggttatcgc atacgtttgg
    10621 taatctttta gcttctaatt tcgtcatgtg cctgtgttag agcgtcttga catactcaag
    10681 tatggtctgc gtcgctctgc cttgtcacat ttagaaattg aaacctaaaa gcatgataca
    10741 aatgcgagag gaccagttct tcgtgcgtgt tcaaatctga cttactgatt atttggtatc
    10801 atgcaaccaa actagaacaa gattcactaa ttgaacacgc ctacaactct ttgaaaaaag
    10861 tgaaaagtgt ttcagaaatg ctcaccttgt tcgtcgtaaa cgtctgaact ttctctggcc
    10921 ttcggtcagg aaagtcctag tcgttctgac gagggtgcgt ctgcgaaatc aaagacttcg
    10981 gacttaccgc ttatttttct tcgagttgct taacggcttt gtatcttgtt caccccgtga
    11041 aaactagtcg ctcgacggct tgataattta acaggagaat taaaaatatg tcacgtattg
    11101 gtaataaagt aattacattg cctgctggcg ttgagcttgc tcaaaacaac ggcgtggtaa
    11161 ctgtaaaagg acctaaaggg gaattgactc gtgaattccc aactgctatt gaaatccgtg
    11221 tggaaggtac agaagttact cttcaccgtc caaacgattc aaaagaaatg aagactattc
    11281 acggtactag ccgtgctaac ctcaacaaca tggttgttgg tgtttctgaa ggcttcaaaa
    11341 aagaacttga aatgcgtggt gtcggttacc gtgctcaatt ggctggtaac aaattgacac
    11401 ttgctgttgg taaatcacat ccagatgaag tggttgcacc agaaggtatt acatttgaag
    11461 ttccaacacc aacacaaatc gtcgtgtctg gtatcaacaa agaagttgtt ggtcaaacag
    11521 cagcttacat ccgtagcctt cgcgctcctg agccatacaa aggtaaaggt atccgctacg
    11581 ttggtgaatt cgttcgccgt aaagaaggta aaacaggtaa ataatagctt gctgaggtgg
    11641 cttagccacc tggctgacta tttctcaaat tgtttcgcaa gaaacagaat catacattaa
    11701 gaggtgaata ttgtgatttc aaaaccagat aaaaacaaaa tccgccaaaa acgccaccgt
    11761 cgcgttcgcg gtaaaatctc tggaactgct gctcgcccac gtttgaacat tttccgttct
    11821 aatacaggca tctacgctca agtgattgat gacgtagcgg gtgtaacgct cgcaagcgct
    11881 tctactcttg ataaagaagt ttcaaaaggt actaagacag aacaagctgt tgttgtaggt
    11941 aaactcgttg ctgaacgcgc ggtagctaaa ggtatttctg aagtggtctt tgaccgcggt
    12001 ggatatctct atcacggacg tgtgaaagct ttggctgaat cagctcgtga aaacggattg
    12061 aaattctaat agggaggaca ctaagaaatg gcattcaaag ataacgcagt tgaaattgaa
    12121 gaacgcgtag tagccatcaa ccgtgttaca aaagttgtta aaggtggacg tcgtcttcgt
    12181 tttgcagctc ttgtggttgt tggtgaccgt aacggtcgcg taggtttcgg tactggtaaa
    12241 gctcaagaag taccagaagc tatccgtaaa gcagttgaat ctgctaagaa aaacatgatt
    12301 gaagtaccaa tggttggtac aacaatccct cacgaagttc gctcagaatt tggcggcgct
    12361 cgtgtattgt tgaaacctgc ttcagaaggt tctggggttg ctgccggtgg tgcaactcgt
    12421 gccgtaatcg aattggcagg tatcgcagat gtgacttcta agtcacttgg ttcaaacaca
    12481 ccaatcaaca tcgttcgcgc aacagttgaa ggtttgaaac aattgaaacg tgctgaagaa
    12541 gtggctgcac ttcgtggcat ctcagtttct gatttagcat aagaaaggag atactcatgg
    12601 ctcaaattaa aatcactttg actaagtctc caatcggtcg caaaccagaa caacgtaaaa
    12661 cagttgttgc acttggactt ggtaaattga actcttcagt tgttaaagaa gataacccag
    12721 ctattcttgg aatggtgaac gctatctctc acttggtaac tgtagaagaa gttaaataat
    12781 ctaacaaata gactaagtcg catagagaaa tctttgcgac ttctagtcca ttttacatat
    12841 ttacatacag cgagtttgtg gaggaagact cttgtacttc acaggcgcta gcatataaaa
    12901 gaggagaaat aataatgaaa cttcatgaat tacaacctgc tacaggttct cgtaaagtcc
    12961 gcaaccgtgt aggtcgtggt acatcatcag gtaacggtaa aacatctggc cgtggtcaaa
    13021 aaggtcaaaa agctcgtagc ggtggcggtg ttcgcccagg ttttgaaggt ggacaaactc
    13081 cattgttccg tcgtcttcca aaacgtggat ttacaaatat caacgctaaa gagtacgcaa
    13141 tcgttaacct tgatcaattg aacgcttttg aagatggtgc agaagtaaca ccagttgtgc
    13201 ttatcgaagc tggaatcgtt aaagctgaaa aatcaggaat taaaattctt ggtaacggtg
    13261 aattgacgaa gaaattgaca gttaaagctg ctaaattctc taaatcagct gaagaagcaa
    13321 tcacttctaa aggtggctca gtagaagtca tctaagaggt ggcagcctat gttttttaaa
    13381 cttttaaaag atgccttaaa ggtaaaattg gtacgtagta aaattctatt tactatcttt
    13441 atcctttttg tcttccgcgt aggaacccac attacagtac caggagtaaa cgcaaagagt
    13501 cttgaggcct tgtcaaacct tccgttcttg aacatgttga gtttggtttc aggaaatgct
    13561 atgcgtaatt tctcagtttt cgcactaggt gttagtcctt atattacagc ttccatcatt
    13621 gttcaacttt tacaaatgga tattttacct aaatttgttg aatggggtaa gcaaggtgaa
    13681 gttggtcgtc gtaaactaaa tcaagcaaca cgctacattt ctttggtact ggcatttgtt
    13741 caatcaattg gtattacagc aggctttaat gctttgtccg gtgcaaaatt gaccaatatg
    13801 ccattaaatt ggcaaacata tttgttgatt ggagcaattt tgacaacagg ttcgattatt
    13861 gtaacctggc tgggggaaca gatttctgaa aagggctatg gtaatggtac atcaatgatc
    13921 atctttgcag ggattatttc atcgttacca ggaacttttc acgagattta cattgatcgc
    13981 tttgttaata ttgaatcaag tcgtttgggg gaatcagcaa tctttgttgc tgcactcgtc
    14041 gtattaatat tctttgttgt gtattttaca acttttgtac aacaagcaga atataaatta
    14101 ccaattcaat atacaaaacg tgcacaagga gcaccttcta gctcatattt accgctgaaa
    14161 ttgaatccag caggagttat tcccgtaatc tttgcaggct caatcactgc agttccaact
    14221 tctttgattc aatatttcgc aagtcaaaat aagagtgccg ggtggttatt gacggttcag
    14281 gaatactttg attattcaac tgctaaaggt atgattgtat atgcaggttt aattattgcc
    14341 tttacattct tctacacctt tgttcaagta aatcctgaga agactgcaga aagccttcag
    14401 aaaagtgcag cctatatcca tggagttcgt cctggtaatg gcactgaaca gtttttgtca
    14461 aaattattga caagattggc agtaatcggt gcactcttct tgagttttgt agctttgctg
    14521 cctatccttg cgcaaaatct ctttgggctt tcttcaagca ttgcgttcct tggtacaagt
    14581 ttgattatcg taatctctac aagtatcgaa ggcatcaagc aattagaagg ctaccttctt
    14641 aagagaaaat atgtaggttt cttagaaatt acagaataga atataggttg acctagtcaa
    14701 ccttctattt tgtttttaga taagtttgtc aataatggca aatttatgtg aaaacaaaat
    14761 aaggagtttg tcatgaatct tttaattatg ggtttaccag gtgctggtaa agggacacaa
    14821 gcggccaaga ttgttgagaa attcaatgtt gctcatattt caacaggaga tatgttccgt
    14881 gcagcaatgg ccaatcagac tgaaatgggt attcttgcca agtcgtacat tgataagggt
    14941 gacctcgttc ccgatgaagt tactaatggc attgtcaaag aacgtttggt tcaggatgac
    15001 atcaaggaaa aaggcttttt gcttgatggc tatcctcgta cgattgaaca agcccatgcc
    15061 ttggatgaaa atttggctga cttaggaatt gagttgcaag gtgttatcaa tattgagatt
    15121 gatccttcaa aattggtaga gcgtctcagc ggtcgtatta ttcataaaga aactggagag
    15181 actttccaca aagtattcaa tccacctgtt ggagactaca aagaggaaga cttctatcaa
    15241 cgtgaagatg acaagccaga atctgtcaaa cgtcgtttag aggtcaatat cgcacaagga
    15301 cagccaatca ttgatcatta tcgtgcaaaa gggctggtcc atgatattga aggtgaccaa
    15361 gacattgacc ttgttttcca agcaattgat acagtactat caaaattgca ataaactaat
    15421 agatttggct tgcatttttt atacaaagat gataaaatag cctagtctga cttataattg
    15481 ttacctctgt gttcagagga catcaaatcg atatttagag aggggttact tttgcatggc
    15541 aaaagaagat gtgattgaaa ttgaaggcaa agtagtcgat acaatgccaa atgctatgtt
    15601 tactgttgag ttggagaatg gacaccaagt ccttgcaact gtttcaggta aaatccgtaa
    15661 aaactacatt cgtattttgg tcggtgaccg cgtaactgtt gagcttagtc catacgactt
    15721 gacacgtgga cgtatcacat accgctttaa atagtcgaaa tacttggagg gattaaacat
    15781 gaaagtaaga ccatcggtca aaccaatttg cgaatactgc aaagttattc gtcgtaatgg
    15841 tcgtgttatg gtgatttgcc cagcaaaccc taaacacaag caacgtcaag gttaagaaat
    15901 agaaaggaga aaacatggct cgtattgctg gagttgacat tccaaatgac aaacgtgtag
    15961 ttatttcatt aacttatgtt tatggtatcg gtcttgcaac atctaaaaaa attcttgcag
    16021 ctgcaggtat ttcagaagat gtacgcgtga aagatttgac atcagatcaa gaagatgcta
    16081 ttcgtcgtga agttgatgca atcaaagttg aaggtgacct ccgtcgtgaa gttaacttga
    16141 acatcaaacg tttgatggaa atcggttcat accgtggtat ccgtcaccgt cgcggacttc
    16201 ctgtccgtgg acaaaacact aaaaacaatg cccgcactcg taaaggtaaa gctgttgcga
    16261 ttgcaggtaa gaaaaaataa tataggaggt agaaaaattg gctaaaccaa cacgtaaacg
    16321 tcgtgtgaaa aagaatatcg aatctggtat tgctcatatt cacgctacat ttaataacac
    16381 tattgttatg attactgatg tgcatggtaa cgctattgct tggtcatcag ctggtgctct
    16441 tggtttcaaa ggttctcgta aatctacacc attcgcggct caaatggctt cagaagctgc
    16501 tgctaaatct gcacaagaac acggtcttaa aacagttgaa gttaccgtta aaggcccagg
    16561 ttcaggtcgt gagtctgcta tccgcgctct tgctgccgct ggtcttgaag taacagcaat
    16621 tcgtgatgtg actcctgtac cacacaatgg tgctcgtcct ccaaaacgtc gccgtgtata
    16681 atcatacatt ccatacacag cttttcgttt aagagggagt aagaaatgat tgagtttgaa
    16741 aaaccaacaa taacaaaaat tgatgaaaat aaagattacg gcagatttgt aatcgaacca
    16801 ttagaacgtg gttacggtac aactcttggt aactctcttc gtcgtgtact tcttgcctca
    16861 cttccaggtg ctgcagtgac atcgattaaa attgatggtg tactccacga attcgacaca
    16921 gttccaggtg ttcgtgaaga tgttatgcaa attattctta acatcaaagg cattgctgta
    16981 aaatcttatg tcgaagacga aaagaaaatt gaacttgatg tagtaggtcc agctgaagta
    17041 acagcaggag acattcttac agatagtgac attgaaattg taaaccctga ccattatctc
    17101 tttaccattg ctgatggtgc cacttttaaa gctgttttga ctgtcaattc aggtcgtggt
    17161 tatgtaccag ctgaggataa taagaaagat gatgcaccag tgggaacact tgctgtagat
    17221 tctatctata cgccagtgaa gaaagtcaat tatcaagttg aaccagctcg tgttggtagc
    17281 aacgatggtt ttgacaaatt aacacttgaa atcaacacaa atggcaccat tgttccagaa
    17341 gatgctttag gtctttctgc acgtattttg atggagcact taggtctctt cactgacttg
    17401 actgaagttg caaaatctgc agaagtgatg aaagaagctg aagtggcttc agatgatcgt
    17461 atgcttgatc gtacgattga agaattggat ctatctgttc gctcatataa ctgtctgaaa
    17521 cgtgcaggca ttaacactgt attcgacttg acagagaaaa ctgagccaga aatgatgaaa
    17581 gtgcgcaatc ttggtcgcaa gagtcttgaa gaagttaaag ttaaattggc tgatcttggt
    17641 ctaggattga aaaaagataa ataatatagg aggaaatcat ggcataccgt aaactaggac
    17701 gcactagctc acaacgtaaa gcaatgttgc gcgatttgac aactgacctt ttgatcaacg
    17761 aatcaatcgt tacaactgaa gctcgtgcta aagaaatccg taaaacagtt gaaaaaatga
    17821 tcacacttgg taaacgtggt gatttgcacg cacgtcgtca agcagcagca tttgttcgta
    17881 acgaaattgc atctgaaaac tatgatgaag caactgataa gtacacttct actacagcac
    17941 ttcaaaaatt gttctctgaa attgcacctc gttatgcaga acgtaatggt ggatatactc
    18001 gtatcctcaa aactgaacca cgtcgtggtg atgctgcacc aatggcaatt atcgaacttg
    18061 tataagatca tcaattttgt tgagtgttat gatgatggaa tcctattctg attcttagtc
    18121 tagctctggt ctaccgctga gcttatctca gcggtaacac tcatcatgtt gattggacgc
    18181 ttgtttacga aattactcta agatagaata atttcgtaag caggcgtttt ttaatagatg
    18241 atacatatca gaaacagagc atttacaaga agaacgaatc aatagttact cgtggtgcta
    18301 gttcatttca aaatacaata aaaagcccaa atggactata ctgtaagtga cgaaacatac
    18361 aggaggttag tccaaatgag ccacttacag tataccgcta aatctcatca tttgcaatgg
    18421 aatgtacgcc aattatcaca aatttgtcat cacttctaca acaactattg cccggattcc
    18481 ttcaaatatc ggcgaaatgt tggcttagtt aaagtttcag atgaatcaat ccttgtctta
    18541 cttttgttac aagctgaact aggtatgaca tcacagcgcc atttctacac catctgccac
    18601 ctcttccctt gcggtcagtt actagaaaga agccgtttca atcgtcggtc aaaacaattg
    18661 atttggctcg tccaatcaat ccgaaaagct atgagtgaaa tgcttccatc tggtaaacta
    18721 gccattatag atagttttcc tttgccaacc tgttcgcaat cacagagcaa ccatttttaa
    18781 aggattagcc gatattggct acaacgcctc taaacatctt tggttctacg gcttcaaagt
    18841 tcatatgcta gtgactttat ctggttatat tttgaactat gttgtcacgc cagcctctgt
    18901 ccatgatatc aaggtggttt atgagctatt agaggggtgt aagcaatcgg ttatcttagg
    18961 tgatctaggc tacctaagca gtgagctcaa aaaggactta gagcaagaag gttaccatct
    19021 atggacgccg tttcgtaaaa acatgactgg gtccgaagaa cataacaatt ggaaagtaat
    19081 ggccatgaga cgaactgttg aaacacgttt ttctgaactg tgtcgtctct ttgacatcga
    19141 acatacatta gctagaagcc tagc
//
LOCUS       DBNFEG010000017        19026 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.017, whole genome shotgun sequence.
ACCESSION   DBNFEG010000017 DBNFEG010000000
VERSION     DBNFEG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19026
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.017"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            21..971
                     /locus_tag="AC1PNG_000441"
     CDS             21..971
                     /locus_tag="AC1PNG_000441"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 family peptidase"
                     /protein_id="HIP6700977.1"
                     /translation="MKTLKKGDHIRVVSPSSSIERIGGFEANLAAKEKLEDLGFKLSF
                     SEHYFENDIFDSAPIASRVADLEVAFADESVDAILTTIGGFNCNELLPYLDFDLIARH
                     PKIFCGYSDTTALLNAIYAKTGMPTYMGPAYSSFKMDLGQQYQTEAWLKAVTQDSFDL
                     TPSPEWSSDAWYLPDAPRTFYPTEWKVYNPGQASGIAIGGNISTLNLLTGTEFAPRPD
                     EYILFLEEAEDDDYLIIARHLTTLLQAYPNPQALVFGRFPKETKMTEEILLAILDKHP
                     ILKTIPVLYDLDFAHTQPLFTITIGGQVELDTKAFSIRFS"
     gene            complement(1006..1371)
                     /locus_tag="AC1PNG_000442"
     CDS             complement(1006..1371)
                     /locus_tag="AC1PNG_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HIP6700978.1"
                     /translation="MYQVIKMYGDFEPWWFLDGWEEDVTSRAAFDRYEDALNAFQKEW
                     VRLSEDFPMKKSKNGTMVAFWDESDQHWCEECDEYLQRYHSLMLVEARENLPAGFIKQ
                     PTQPRVRPCKLKQNNCVNE"
     gene            complement(1520..5167)
                     /gene="rpoC"
                     /locus_tag="AC1PNG_000443"
     CDS             complement(1520..5167)
                     /gene="rpoC"
                     /locus_tag="AC1PNG_000443"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIP6700979.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERD
                     GLFDEVIFGPTKDWECSCGKYKRIRYKGITCDRCGVEVTRAKVRRERMGHIELKAPIS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLPKEIAALKEELKTASGQKRIKAVRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMVQLDGGRFAASDLNELYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIAGNVKAAKRLIE
                     RGDDRIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMVFKDADEAVMAYRNGYVHLHTRVGIATDSLDKPWKDNQKHKIMMTTVGKIL
                     FNAIMPEGLPYLQEPNNANLTEGTPDKYFLESGSDIKAAIAELPINPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIEESHERVEQ
                     IKKQFRRGMITDDERYAAVTDEWRSAKEKLEKRLVEKQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMSAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLDIRSITDGKEMIEPLEERLQGRYTKKTVKHPE
                     TGAVIIGPNQLITEDIAREIVNAGVEQVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNSDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGEVIAIEEDASTRTKKVFVKGKTGEGEYVVPFTARMKVEVGDQVARGAALTEGS
                     IQPKRLLEVRDVLAVETYLLSEVQKVYRSQGVEIGDKHIEVMVRQMLRKVRVMDPGDT
                     DLLMGTLMDISDFTDANAETVIAGGVPATARPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKRDNLLGLKENVIIGKIIPAGTGMARYRNLEPQAINEVEIIEDTVAEEL
                     AAEAELEAVTE"
     gene            complement(5345..8917)
                     /gene="rpoB"
                     /locus_tag="AC1PNG_000444"
     CDS             complement(5345..8917)
                     /gene="rpoB"
                     /locus_tag="AC1PNG_000444"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIP6700980.1"
                     /translation="MAGHEVQYGKHRTRRSFSRIKEVLDLPNLVEIQTDSFQDFLDYG
                     LKEVFEDVLPVSNFTDTMELEFVGYELKEPKYTLEEARAHDANYSAPIYVTFRLVNKE
                     TGEIKTQEVFFGEFPIMTEMGTFIINGAERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDSKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTIEKDIHKNPADSRTDEALKEIYERLRPGEPKTAESSRSLLTARFFDPRRYDLAPVG
                     RYKINKKLNLRTRLLNQTLAEHVINGETGEIVLEAGTVLSRDVLEKVEAQFDELNLVE
                     YIPNDNAVLLEPVLLQKFKIVAPKDPERVVTVIGNANPAENVRTVTPADILAEMSYFL
                     NLAEGLGRVDDIDHLGNRRIRAVGELLANQVRIGLTRMERNLRERMSVQDNEVLTPQQ
                     IINIRPVTAAIKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVRD
                     VHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFIQTPYRKIDRATGTVTNEIVW
                     LTADEEDAYIVAQSTSPLDENNRFVDKIVMGRHQGNNQEFPADSADFMDVSPKQVVAV
                     ATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAIIAQHD
                     GKVVYADADKVEVRREDGSLDVYHISKFRRSNSGTAYNQRTLVKLGDIVEKGDFIADG
                     PSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDTK
                     LGPEEITREIPNVGEDALRNLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERLL
                     HAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRANGDELQSGVNMFVRVYIAQKRKI
                     KVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHLG
                     MAARNLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGVM
                     YMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVLQ
                     EILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDNNE
                     VELRDLDEGEDDDIIHVDDLEKARAKAAADAAAAFAAEEAEGKE"
     gene            complement(9474..11897)
                     /gene="pbp1b"
                     /locus_tag="AC1PNG_000445"
     CDS             complement(9474..11897)
                     /gene="pbp1b"
                     /locus_tag="AC1PNG_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1B"
                     /protein_id="HIP6700981.1"
                     /translation="MATKSDKQDFKKKISALVLGDVVGVFLRTLKLLFNSVAVLVFLF
                     GLFGAGIGIGFVVSLFDDVKIPKTEELVAKVSEVSRISTVTYSDGSLVSEVNSDLLRV
                     PITSEEVSNYLKQAVIATEDETFETHNGVVPKAVLRAALGSVGLGSSSGGSTLTQQLI
                     KQQLVGDAPTFTRKANEIVSALALERNMTKEEILTIYLNVSPFGRNNQGRNIAGVEAA
                     AQGIFGKPAKDLTVPQAAFIAGLPQSPIVYSPYASDGTRKSDEDMVYGVERYQDVLFN
                     MYRASFLTKEEYETYKAYDIKQDFIAPAPVMADTKDYLYYKVMEEAQEVMFDYLVKRD
                     KVSKNDLKNDETKASYEELAKQELSQGGYTIKSTVDQGIYAAMQSVVANYGSVLDDGN
                     EYVETGSVLIDNATGAILGFVGGRDYSTNQNNHAFDTLRSPASTIKPLLAYGIAIDQG
                     LIGSASILSDYPTNFSSGQPIMYGSGRGTGMMNLQTAIDRSVNIPAFWTYKMMRNAGV
                     DAKAYMDKMNYHIPMYDIESVPLGGGVEISVLTNTNAYQTLANGGVYNKHYIVESITA
                     SDGTVVYQHEAAPVQVYSKATASIMNQLLRQVVNSGYTTTFKSRLSGLNPQAASSDFV
                     GKTGTSNEVNDVWLMLSTPRVTLGTWAGNDDNSEMYVWTGYHNNSQYVAHMVDALYNV
                     NADMFAGKFELDSSVIASSVVASTGQRAGTTQVNGRQVTVGGAMTTSYWAKNGAPVTS
                     YNFMVGGTDSDRAQAWNTIIGSQTPTSSSSTQRSSSTRSSTSSSANNTNQSSETQTAT
                     SEQANNDSP"
     gene            12051..13307
                     /gene="tyrS"
                     /locus_tag="AC1PNG_000446"
     CDS             12051..13307
                     /gene="tyrS"
                     /locus_tag="AC1PNG_000446"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIP6700982.1"
                     /translation="MNIFEELKARGLVFQTTDEEALVKALTEGQVSYYTGYDPTADSL
                     HLGHLVVILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVTKIQ
                     GQLSRFLDFENGDNKAEMVNNYDWFSDISFIDFLRDVGKYYTVNYMMSKDSVKKRIET
                     GISYTEFAYQIMQGYDFYELNDKYNVTLQIGGSDQWGNMTAGTELLRRKADKSGHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDE
                     IAEIEKQFDAARHERLAQKILAREVVTLVHGEEAYNQALNITEQLFAGNIKNLSAKEL
                     KQGLSNVPNYAVQAEDNLNIVELLVTSGIVTSKRQAREDVQNGAIYVNGERVQDLDYT
                     LSDSDKIDGELTVIRRGKKKYSVLTY"
     gene            13365..13742
                     /locus_tag="AC1PNG_000447"
     CDS             13365..13742
                     /locus_tag="AC1PNG_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIP6700983.1"
                     /translation="MTCIFCHQIEEIDILYQTEYFKVVWDIDPIQTGHLLIISKEHYD
                     TLSQIPSAVRYELSDLEVFLTGKLCQTLAIDGVTTARNDRLFDAGTHFHVHLIPRFRS
                     DGFWDQISLAQVQLDLNAFLKAL"
     gene            complement(14035..14244)
                     /locus_tag="AC1PNG_000448"
     CDS             complement(14035..14244)
                     /locus_tag="AC1PNG_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIP6700984.1"
                     /translation="MKQTLKLKNLSCQNCVKHVTNHFLELDGVEEVKIQLEEQLAEVE
                     TTVAYDLEQYQEVLEDTIYEVEELV"
     gene            complement(14241..14687)
                     /locus_tag="AC1PNG_000449"
     CDS             complement(14241..14687)
                     /locus_tag="AC1PNG_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HIP6700985.1"
                     /translation="MEQTISAAEWQVMRVLWAQPGATSQEIIQALQEGFDWQATTIKT
                     LLGRLRKKNYLRMAKETSKYHYYPLISEEEHLQGQVELLLATICSTKQGQLVEKLLDT
                     GIFSQKSLEKLASKISQLQRTAPEQIACRCLAGQCTCGHHAKGEKT"
     gene            complement(14787..16298)
                     /locus_tag="AC1PNG_000450"
     CDS             complement(14787..16298)
                     /locus_tag="AC1PNG_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter solute-binding protein,
                     Zn/Mn family"
                     /protein_id="HIP6700986.1"
                     /translation="MKKVGLLFLSVSALLLGACGNSTASEDGKLNIVTTFYPVYEFTK
                     QVAGDEANVDLLVKAGTEVHGYEPSAKDIARIQEADAFVYENENMETWVHDVEKSLDT
                     TKVNVISATEGMLLLPGGEEEHKGHDHSGERHSHAYDPHVWLSPERAITLVENIRDSL
                     VAKYPEKKDAFETNAAAYIEKLDALDAKYSETLSAAKQKYFVTQHTAFAYLALDYGLK
                     QVSITGVAADEDPTPSRLAELTEYINKYGIKYIYFEENASKSVAETLAKETGVQLDVL
                     NPLESLTDEDMKNGKDYISVMEDNLTALEKTTSQEGSEILPEEGAETAQTVYNGYFED
                     SAVKDRTLSDYAGEWQSVYPYLLDGTLDQVWDYKAKIKGGMTAEEYKAYYDTGYKTDV
                     DQINITDNTMEFVVGDKKEKFTYKYVGYKILTYKKGNRGVRFLFEATDANAGNYKYVQ
                     FSDHNIAPVKTGHFHIYFGGESQEKLLEELENWPTYYPVGLTGLEIGQEMLAH"
     gene            complement(16308..17120)
                     /locus_tag="AC1PNG_000451"
     CDS             complement(16308..17120)
                     /locus_tag="AC1PNG_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIP6700987.1"
                     /translation="MFDLSVFHYDFMQRAFLAIIAMSLFSPILGVFLILRRQSLMSDT
                     LSHVSLAGVAFGLVLGISPTLSTVLVVIVAAVFLEYLRTIYKNFMEIGTAILMSTGLA
                     ISLIVMNKSGGKSGLSLEQYLFGSIVTISQEQVIALFTIAVIVIVLTLLFLRPMYILT
                     FDEDTAFVDGLPVRAMSIAFNVVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKSF
                     KAVIFTGMGIGFFGMVMGLLTSYYAETPASASITLIFISIFLLVNVVQKFKK"
     gene            complement(17113..17817)
                     /locus_tag="AC1PNG_000452"
     CDS             complement(17113..17817)
                     /locus_tag="AC1PNG_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIP6700988.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHIHYYLDSGEFVTLTGENGAAKTT
                     LIKATLGILKPKQGKVSIAEKSIKGKKLRMAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RQGWFRRLTAHDEEHVRISLESVGMWEHRDKRLGALSGGQKQRAVIARMFASDPDIFI
                     LDEPTTGMDAGTKDAFYQLMHHSAKKHGKSVLMITHDPDELNKYADRNIHLVRDQQSP
                     WRCFNVHEADEEVAHV"
     gene            complement(17818..18261)
                     /locus_tag="AC1PNG_000453"
     CDS             complement(17818..18261)
                     /locus_tag="AC1PNG_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HIP6700989.1"
                     /translation="MNRIVLEIEKYLHEIVLSSENQLEILVGSCQSTVKLTNTQEHIL
                     MLIEKAAYTNTEIAKELNVSQAAITKATKSLVAQGLLVAVRDDKDARIVRFSLTEAAK
                     PIATEHAHHHAHTLEAYEELLEHYSLEEQESIARFLSELVEKIRK"
     gene            18470..19010
                     /locus_tag="AC1PNG_000454"
                     /pseudo
     CDS             18470..19010
                     /locus_tag="AC1PNG_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377895.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         5484 a         4524 c         3685 g         5333 t
ORIGIN      
        1 aaaaaaactt ggaggttcct atgaaaacat taaaaaaagg cgaccacata cgtgtagtca
       61 gtccctcttc atctattgaa agaattggtg gttttgaagc caatcttgct gccaaggaaa
      121 aattggaaga cctgggcttc aaactctctt tctcagaaca ttattttgaa aacgatattt
      181 tcgattccgc tccaattgcc agtcgtgttg cggacttgga agtagccttt gcggatgagt
      241 cagtcgatgc cattctgaca accattggcg gtttcaactg caacgaactc ttgccctatc
      301 tggattttga cctaattgcg cgtcatccaa agattttctg tggttattcg gacacaactg
      361 ccctgctcaa cgccatctac gccaagacag gcatgccaac ctacatgggt ccagcttatt
      421 caagtttcaa aatggatttg gggcaacagt atcaaacaga agcctggctc aaggctgtga
      481 cacaagactc ctttgacttg accccgagtc cagaatggtc tagcgacgct tggtacctac
      541 cagacgcccc tcgcaccttt tacccaacag aatggaaagt ctacaatcca ggccaggcct
      601 ctggtattgc cattggcgga aacatctcaa ccctcaacct gctgactgga acagagtttg
      661 ctcccagacc agacgaatat attctatttt tagaggaggc ggaagacgac gattatctca
      721 ttatcgctcg tcacttgact acacttttac aagcctatcc aaatccacag gctcttgtct
      781 tcggtcgttt tcctaaagag accaagatga cggaggagat tttgctggcg attttggaca
      841 agcaccctat tctcaaaacc atcccagttc tctacgattt ggattttgcc catacccagc
      901 ctctgtttac cattacaatc ggtggtcaag tggagctcga caccaaagcc ttttctatca
      961 gattcagcta aaaaggattg acccgcaaag gtcaatcctc ttttattatt catttacgca
     1021 attattttgt tttaatttgc aggggcgtac acgaggttgc gtaggttgct taataaaacc
     1081 agccggtaaa ttttctctcg cttctaccaa catcagcgaa tgataccgct gcaaatattc
     1141 atcgcattcc tcgcaccaat gctggtctga ttcatcccaa aaagccacca tcgttccatt
     1201 cttacttttc ttcattggaa aatcctctga tagccgtacc cactcttttt gaaaggcatt
     1261 cagtgcatcc tcatagcgat caaaagcagc cctactggta acatcctctt cccaaccatc
     1321 taagaaccac cagggctcaa aatctccata cattttaatt acttgataca taacacatac
     1381 ttcctttttc gtacacatta taccgaattc gtctgattta agaaaacatt ctgcttgtta
     1441 attccttatc aagttgtgtg agcgctttat ttctgaacga aagaaaacac atccctcaga
     1501 aagagatgtg tctcaaatct tattcagtta cagcttcaag ctctgcttct gctgcaagtt
     1561 cttcagcaac tgtgtcttca atgatttcaa cttcattgat ggcttgtggt tcaagattac
     1621 ggtagcgggc catacctgta cctgctggga taatcttacc gataataacg ttctctttaa
     1681 gaccgagaag gttgtcacgt ttaccacgga tagcagcatc tgtaaggaca cgtgttgttt
     1741 cctggaagga tgcggcagac aagaatgagt tggtttcaag agacgctttt gtgataccca
     1801 taagaactgg acgagccgtt gctggaacac caccagcgat gacagtttcc gcattggcat
     1861 cagtaaagtc tgagatatcc atgagggtac ccatgagcag gtctgtatct cctggatcca
     1921 tgacacgaac tttacgaagc atttgacgaa ccattacctc gatgtgcttg tcgccgattt
     1981 ctacaccctg gctacggtaa actttttgaa cttcagaaag aaggtaagtt tcaaccgcca
     2041 agacatcacg gacttcaagc aagcgttttg gttggataga accttcggta agggcggctc
     2101 cacgcgcgac ttggtcacca acttcaacct tcatacgggc tgtaaatgga accacatatt
     2161 cgccttcgcc agttttaccc ttaacaaaga ctttcttggt acgagtagaa gcatcttctt
     2221 caatagcgat aacttcacct ttaacctcag tgataaccgc ttcccctttc ggattacggg
     2281 cttcaaagat ctcttggaca cgaggaagac cctgcgtaat atcgctgttt gaggctacac
     2341 cacccgtgtg gaaggtacgc attgtaagct gtgtaccagg ctcaccgatc gattgggcag
     2401 cgattgtacc aactgcttca cccacttcaa ccgcatcacc tgttgccaag ttgataccat
     2461 aacaatgacg gcagacaccg tgacgagtgt tacatgtaaa tacgctacgg atagttactt
     2521 gttcaacacc tgcattgacg atttcacggg caatatcttc cgtaatcaat tggtttggac
     2581 caatgataac ggcacccgtt tcaggatgtt taacagtttt cttagtgtaa cgaccttgca
     2641 aacgctcttc aagtggctcg atcatttcct tgccatctgt gattgaacgg atatcaagac
     2701 cacggtctgt tccacagtcg tcttcacgga taataacgtc ttgggcaacg tcaaccaaac
     2761 gacgagtcaa gtaacctgag tcggctgtct tcaaagccgt atccgtcatc cccttacggg
     2821 caccgtgagt tgagaagaac atttccaaaa cagaaagacc ttcacggaag ttagacaaga
     2881 taggcaattc catgatacgt ccgttcggag ctgacatcag accacgcata ccggccaact
     2941 gggagaagtt ggaaatgtta ccacgggcac cagagtccat catcataacg attggattct
     3001 tcggatcttg tttttcaacc aggcgttttt ccaatttttc cttagctgaa cgccattcat
     3061 ctgtaacagc tgcataacgc tcatcgtctg taatcatacc acgacggaat tgtttcttga
     3121 tttgttctac acgttcgtga gattcttcaa tgatttcagc cttgttgtcg ataactggga
     3181 tatcggcaat acccactgtc aaaccagcga gtgttgagtg ataataaccc aagtctttca
     3241 aacggtcaag aagggctgat gtctcagttg tacggaaacg cttgaagatt tcagcgatga
     3301 tgttaccaag atttttcttc ttgaatggtg ggttgattgg caattctgca atggcagcct
     3361 tgatatctga acctgattcc aagaagtatt tatcaggagt tccttctgtc aagttagcat
     3421 tgtttggctc ttgcaagtat ggaagacctt ctggcataat cgcgttgaac aagatttttc
     3481 cgacagttgt catcataatc ttgtgttttt ggttgtcttt ccaaggttta tcaaggctat
     3541 ccgttgcgat accaacacga gtatgcaagt gaacatagcc attgcggtaa gccataacag
     3601 cctcatccgc atccttgaag accatacctt caccttcacg accagcatct tccatggtca
     3661 agtagtagtt acccaaaacc atatcctgag atggtgttac gacaggttta ccatctttag
     3721 ggttaaggat gtgttctgcc gcaagcataa ggatacgtgc ttctgcttgt gcttcttctg
     3781 acaatggaac gtgaatcgcc atctggtcac cgtcgaagtc ggcgttgtag gcttcacaga
     3841 caagcgggtg caaacgaagg gctttaccgt cgatcagaac tggctcaaaa gcctggatac
     3901 ccaaacggtg aagggtaggt gcgcggttca aaagaactgg gtgttctttg atcacttctt
     3961 ccaagatatc ccagatacga tcatccccac gttcaatcaa acgttttgca gcttttacgt
     4021 taccagcaat atcacgggca acgatttcgc gcataacaaa cggtttgaag agctcgatag
     4081 ccatttcgcg tggcacacca cattggtaca ttttaagcgt tggaccaaca gcgataacgg
     4141 aacgtcctga gaagtcaaca cgcttaccaa gcaagttttg acggaaacgt ccttgcttac
     4201 ctttaagcat gtggctgagt gatttaagtg gacggctacc tggtcctgta attggacgac
     4261 cacggcgacc gttgtcaatc aaagcatcca cagcttcttg gagcatccgt ttttcgtttt
     4321 gtacgatgat acctggagcg ttaagttcca agagacgagc caaacggttg ttacggttga
     4381 taacacggcg gtagagttcg ttcaagtcag acgccgcaaa acggccacca tccaactgaa
     4441 ccatcggacg caaatctggt ggaataactg gaaggatgtt gagaatcatc cactcaggct
     4501 tgttaccaga tttgtagaat gcatccaata catctaagcg acgaactgct ttaatgcgtt
     4561 tttgaccaga agctgttttc aattcttctt tcaaagctgc gatttctttt ggaagatcga
     4621 cttgtttcaa gaggtcttgg atggcttctg cacccatttt agcaacgaat gaaccataac
     4681 catattcacg caaacgctca cggtattcgc gctctgtcat gattgacttg tgctcaagcg
     4741 gtgtatcttt aggatcaatc accacgtaag ccgcgaagta gataacttcc tcaagcgcac
     4801 gtgggctcat atccaaggtc aagcccatac gacttggaat acctttgaaa taccaaatgt
     4861 gtgaaattgg tgctttcaac tcaatgtgtc ccatacgttc acgacgtact tttgcacgag
     4921 tcacttcaac accacagcgg tcacaagtaa tccctttata acggatacgt ttgtatttac
     4981 cgcatgaaca ctcccagtct tttgttggac cgaagatgac ttcgtcaaaa agtccatcac
     5041 gttctggttt aagtgtacga tagttgattg tttcaggttt tttaacctca ccgtaagacc
     5101 atgaacgaac cttacttggt gaagctaacg tgatttgcat acttttaaat cgatttacgt
     5161 caaccactaa taatcccttt cttttcttgt gagaggttgg attcccagac ctcaaattct
     5221 tgaacaataa tcagcagaat ggatagctgg taagctattt ttctataaat cagacggtgg
     5281 aagaccgtcg gcccttatct atttcagttc tgaggtttgg accaacttgt tccatccatt
     5341 ctaattattc tttgccttct gcttcttctg ctgcaaaggc tgcggctgcg tcagctgcgg
     5401 cttttgcacg tgctttttca agatcatcta cgtggatgat gtcatcatct tcaccttcat
     5461 caaggtcacg caactctact tcattgttgt cttcatcaag gacacgcata tccaaaccaa
     5521 gtgattgcaa ttctttgaca agaacgcgga atgattctgg aacacctggt tttggaattg
     5581 gtttaccttt ggtgatggct tcataggctt taagacggcc tgtcacatca tctgacttat
     5641 aagtcaagat ttcttgaagg acgtttgaag caccgtaggc ttcaagggcc caaacctcca
     5701 tctcaccgaa acgctgacca ccaaactgag ccttacctcc gagtggctgt tgggtaacga
     5761 gtgagtatgg tccgactgag cgggcgtgga gtttatcatc aaccatgtgg tgaagcttga
     5821 tcatgtacat gacaccgaca gatacacggt tgtcaaatgg ctcacctgta cgtccatcgt
     5881 aaagaatggt tttggcatct gaatccatac ctgcttcttt aacagttgac caaagatctt
     5941 ctgaacttgc accatcgaaa actggtgttg cgatatggat gcccaagttg cgagccgcca
     6001 tacccaagtg aagttccata acctgaccga tgttcatacg tgatggcacc ccgagtgggt
     6061 tcaacatgat gtcaactggt gttccgtctg gaagatatgg catatcctca acaggtacaa
     6121 tacgtgaaac gacacccttg ttaccgtgac gaccggccat cttatctccg accttgatct
     6181 tacgtttttg agcgatgtaa acacgaacaa acatgttaac acctgattgc aattcatcac
     6241 cgttggcacg agtaaagatt ttcacatcac gaacgacacc atcggcaccg tgaggtacac
     6301 gaagagaggt atcacgtact tcacgtgact tgtcaccgaa gattgcgtgc aagagacgct
     6361 cttcagcaga aagatctttt tcaccttttg gtgtaacttt accaacaaga atgtcgccct
     6421 ctttaacttc ggcaccaata cggataatac ccatttcgtc caagttgcga agagcatctt
     6481 caccaacgtt tggaatttcg cgagtgattt cttcagggcc taacttggta tcgcgtgttt
     6541 ctgattcgta ttcttccaag tgaacagatg tatagacatc gtccttcaca aggcgttcag
     6601 acatgataac cgcatcctcg aagttgtaac cttcccaagt catgtaggca acgatagggt
     6661 tttgaccaag agccatttcc ccattttcca tagaagggcc atctgcgatg aagtcgcctt
     6721 tttcaacgat gtcgcccaat tttacaaggg tacgttggtt gtaggcagta ccagagtttg
     6781 aacgacggaa tttagaaatg tggtagacat caagcgatcc atcttcacga cgcacttcta
     6841 ccttgtccgc atctgcgtaa acaaccttac catcgtgttg agcgataatt gccgcacctg
     6901 agtcgtgagc agcctggtat tccataccag taccaacgta aggtgctttt ggatcaatca
     6961 atggaacagc ctgacgttgc atgttggccc ccatgagggc acggttggag tcatcgtttt
     7021 ccaagaaagg aatacatgct gtcgcaacgg caactacctg cttaggtgaa acgtccatga
     7081 aatctgctga atctgctggg aactcttggt tgttaccttg gtgacgtccc ataacaatct
     7141 tatcaacgaa acggttgttt tcatcaagtg gcgatgttga ttgggctaca atataagcat
     7201 cttcttcatc ggctgtcaac caaacgatct catttgtaac tgtaccagtt gcacggtcaa
     7261 tcttgcggta tggtgtttgg atgaaaccat acttgttgag atgcccataa gatgacaagt
     7321 tgttgatcaa accgatgttt ggtccctcag gcgtttcaat cggacacata cgaccatagt
     7381 gagtgtagtg aacgtctcga acctcataac cagcacggtc acgagtcaaa ccaccaggtc
     7441 ccaaggctga caaacggcgt ttgtgagaca actcagaaag tgggttgtgt tggtccatga
     7501 actgtgacaa ctgagatgaa ccaaagaatt ctttgattgc ggctgtaaca ggacggatat
     7561 tgatgatttg ttgtggcgtc aatacttcat tgtcttgaac cgacatacgc tcacgcaagt
     7621 tacgttccat acgagtcaaa ccgatacgta cttggttagc aagcaattca ccaacggcac
     7681 gaatacgacg gttacccaag tggtcaatat catctacgcg accaagtcct tcagccaagt
     7741 tgaggaagta gctcatttca gccaagatgt ccgcaggtgt tactgtacgt acgttttctg
     7801 ctggattagc attaccaatc accgtcacaa cacgttctgg atccttagga gctacaatct
     7861 tgaatttctg caaaagaact ggctcaagaa gaacagcgtt gtcatttgga atatattcta
     7921 ccaagttgag ctcatcgaat tgtgcttcta ctttttcaag cacatcacga ctcaatacag
     7981 taccagcttc caagacgatt tcgcctgtct caccgttaat cacatgctca gcaagtgttt
     8041 ggttgagcaa acgagtacgg aggttgagtt ttttattaat cttgtaacga ccaacaggtg
     8101 ccaaatcgta acgacgtggg tcaaagaaac gagctgtcaa aaggctacga gagctttcag
     8161 ctgtctttgg ttcacctgga cgaaggcgct catagatttc cttaagcgct tcatccgtac
     8221 gagaatctgc tgggttttta tgaatatctt tttcaatggt attgcgaacc aattcgctat
     8281 caccgaagat gtcaaagatt tcatcatcgc ctgagaaacc aagcgcacga acaagcgttg
     8341 tgaacggaat cttacgcgta cggtcaatac gagtgtaggc aatgtctttt gaatctgttt
     8401 ccaattccaa ccaagctcca cggttaggga taaccgttga accataacct actttaccgt
     8461 tcttatctac tttatcattg aagtagacac ctggtgaacg aaccaactga gaaacgatga
     8521 tacgctctgc accgttgatg atgaaggtac ccatttcagt catgattgga aactcaccga
     8581 agaagacttc ttgtgtcttg atctcgccag tttctttatt caccagacgg aaagttacat
     8641 agattggagc tgagtagttc gcatcatgcg cacgcgcctc ttccaatgtg tatttaggct
     8701 ctttcaattc ataaccaacg aattccaatt ccatggtatc cgtaaagttt gaaactggaa
     8761 gtacatcttc aaagacttct ttcaaaccat aatctaaaaa gtcttggaaa gagtccgttt
     8821 ggatttcaac caaatttggt aaatcaagaa cttccttgat tcttgaaaaa ctacgacggg
     8881 tacggtgctt accgtactga acttcatgtc ctgccaaaag ttttctcctt tgtattaaga
     8941 tactaagccg taaacaactc aaagaaaaat aggaaacacg acgacggagg caagcctcct
     9001 aggagctgtt tatctttttt cacagagttg taggcgagtt caattaaaag atacgagacc
     9061 gtgaaaacca aagtgaaaat aagcggtaag tccaaaatct ttgattttgt tgacgcaccc
     9121 tcgtcagaac gactaggact ttcttgacaa atcgtcgaag aaaggccaga cgtttacgac
     9181 gttgggagtg acaaaccaaa gtaaatattt catacacttt ttcacacagg ttttagcccg
     9241 agttcaatta tatacagcca tgtgatgacc aacattcatc ctttttctga atttttagaa
     9301 tctttaagaa tagataacaa ttctcaaaaa aattcctcat agacacaaaa agagcagcta
     9361 aatcttcctt tgaaaaagtt tgatttaact gctgcaagcc ttgtttggac aatatttcaa
     9421 acaagacaca cgacaaataa tttacactat tatagcacaa gtactaggaa aaatcaaggt
     9481 gaatcattat tcgcttgctc actagttgct gtttgcgttt ctgatgactg gttcgtgtta
     9541 ttagctgaag aactcgtgct actccgagtc gaactgcttc gttgggttga gctacttgat
     9601 gttggcgttt gggaaccgat aattgtattc caagcctgtg ctcggtcact atctgtcccc
     9661 ccaaccatga agttatagct cgttactggt gcaccatttt tagcccaata actagttgtc
     9721 atcgcaccac caactgttac ttggcgccca tttacctgtg tcgtccctgc acgttgacct
     9781 gtagaagcaa cgacactaga agcaataaca ctactgtcta attcaaattt tcctgcgaac
     9841 atatccgcat taacattgta aagcgcatca accatatggg ccacatattg tgaattattg
     9901 tggtagcctg tccagacata catttctgag ttatcatcat tcccagccca ggtacctaaa
     9961 gttactctag gggttgatag catgagccac acgtcattaa cttcattact tgtccccgtc
    10021 ttaccgacga aatcagatga tgctgcctgt gggtttaaac cactcaatcg actcttgaac
    10081 gtcgtagtat aaccagagtt gacaacctgt ctcaaaagct ggttcataat acttgcagtt
    10141 gctttagaat agacctgaac cggagccgct tcatgctgat acactactgt tccatcagag
    10201 gcagtaatac tctcaacgat gtaatgtttg ttataaacgc caccatttgc caaagtttga
    10261 taggcattgg tatttgtcaa tactgaaatt tcaacaccac cgcccaacgg tacactttca
    10321 atatcgtaca taggaatatg ataattcatt ttatccatgt acgctttggc atctacacct
    10381 gcatttctca tcatcttata ggtccaaaaa gcaggaatgt taacagaacg gtcaatagcc
    10441 gtctgtaagt tcatcatacc agtaccacgt cctgaaccat acataatcgg ttgtccactt
    10501 gaaaagttcg ttggataatc ggaaaggatg ctggcagaac ctatcaaacc ttgatcaatc
    10561 gcaataccgt aagctaacag aggcttgatg gttgacgctg gcgaacgaag agtatcaaag
    10621 gcatggttat tttggttggt tgaataatcg cgaccaccca caaatcctaa aatggctcct
    10681 gtggcattat caattaacac actacctgtc tcgacatatt cgtttccatc atccaaaaca
    10741 gagccataat ttgccacaac agactgcatc gccgcataga ttccttgatc aacggtactc
    10801 ttaatcgtgt agccaccttg gctcaattct tgcttggcca actcctcata ggatgccttt
    10861 gtttcatcat ttttcaagtc atttttagaa accttatcgc gttttaccag gtaatcaaac
    10921 ataacctcct gcgcttcttc catgaccttg taatagagat aatctttcgt atccgccatt
    10981 acaggagctg gagcaataaa atcttgttta atatcgtagg ctttataggt ttcgtattct
    11041 tccttggtca agaatgacgc ccgatacata ttaaagagaa catcttgata gcgttcaaca
    11101 ccgtaaacca tatcttcatc tgatttacgt gttccatctg atgcataggg agaataaaca
    11161 atcggacttt gaggcaagcc tgcaataaaa gcagcctgcg gaacagtcaa gtctttcgct
    11221 ggtttaccaa aaattccctg agccgcagct tctacccctg caatatttcg tccctgatta
    11281 tttctaccaa atggagaaac atttaagtaa atggtcaaaa tctcttcctt ggtcatattc
    11341 cgctctaatg ccaaagccga aacaatctca ttagccttac gcgtaaaggt tggagcatct
    11401 ccgacaagtt gctgttttat caattgctgc gtcaaggttg aaccaccgct agatgaacct
    11461 agaccaactg atcccagtgc cgcacgcaaa acagccttag gcacaacccc attgtgggtt
    11521 tcaaatgttt catcctctgt cgcaattaca gcctgtttta gataattcga gacctcctcc
    11581 gaagtaatag gtacgcgaag cagatctgaa ttaacttccg acaccaagct accatccgaa
    11641 taggtaacgg tggaaatccg actaacttct gaaaccttcg ctaccagttc ctctgttttg
    11701 ggaatcttaa cgtcatcaaa aagactaacc acgaaaccaa ttcctatgcc cgcaccaaaa
    11761 agaccgaata ggaacaccaa gacagctact gaattgaata ataatttcag cgtccgaagg
    11821 aaaactccta ctacatcccc taagactagg gcactgattt tctttttaaa atcttgctta
    11881 tctgatttag tcgccatatt aactccttat cgttccatta taccaaattt tgtttatttt
    11941 ccgcaatttc gtggtataat agggaggaat tttttaaaat gaagttcggt ccaaaaatca
    12001 tataaaatgg tttatggtcc tgtatattaa acctaaagga gacagcccac atgaacattt
    12061 ttgaagaact aaaagctcgt ggcttggtct ttcaaacgac agacgaagaa gccttggtaa
    12121 aagcattgac agaaggacag gtttcttatt acacaggata cgatccaaca gcagatagtt
    12181 tacacttggg gcacttggtt gttatcttaa ctagccgtcg cttgcagttg gcaggccaca
    12241 agccttacgc cctagttggc ggtgcaactg gtctgattgg cgacccttcc ttcaaggatg
    12301 cggagcgtag cttgcaaacc aaagacaccg tggacggctg ggtaacaaaa atccaaggtc
    12361 agctttctcg cttcttggat tttgaaaatg gtgacaacaa ggctgaaatg gtcaacaact
    12421 acgactggtt ctcagacatc agctttatcg acttccttcg tgatgttggt aaatactaca
    12481 cggtcaacta catgatgagc aaagattctg tgaaaaaacg gattgaaaca gggatttcct
    12541 acaccgagtt tgcctaccaa atcatgcagg gctatgactt ctacgagctt aatgacaagt
    12601 acaatgtgac cctacaaatc ggtggttctg accagtgggg caacatgaca gccggtactg
    12661 aattgcttcg ccgcaaggct gacaagtctg gtcacgtgat gactgttccc ctcatcactg
    12721 actcaacagg taagaaattt ggtaagtctg aaggcaacgc tgtttggttg gatgccgaca
    12781 agacttctcc gtatgaaatg taccaattct ggctcaatgt catggacgat gatgccgttc
    12841 gtttcttgaa gatttttact ttcttgtcat tagatgaaat cgctgaaatc gagaaacaat
    12901 ttgacgctgc ccgtcatgaa cgcttggctc agaagatttt ggctagggaa gtcgtgacat
    12961 tggttcacgg tgaagaggct tataaccaag cccttaacat caccgagcaa ttgttcgcag
    13021 gcaatatcaa aaacctatcc gcaaaagaac tcaaacaggg cctcagcaac gttccaaact
    13081 acgctgtaca ggctgaagac aaccttaaca tcgtggaact tctagttacc tctggtattg
    13141 tcacttcaaa acgccaagct cgtgaagatg tgcaaaatgg tgccatttat gtcaacggtg
    13201 agcgtgtgca ggacttggac tatacccttt ctgacagtga caagattgac ggcgagctga
    13261 ccgttatccg tcgtgggaag aagaagtatt ctgtgttgac ttattaagat ttttcaagga
    13321 aatccaccat gatttccttt tgttctatca ataggagaac agctatgacc tgtatttttt
    13381 gccatcagat tgaagaaata gatattctct accagacaga atatttcaag gtggtctggg
    13441 acattgaccc tattcaaaca gggcatctac tgattatcag caaggaacac tatgacacgc
    13501 tcagccaaat cccttctgct gttcgctatg agctatcgga cttggaagtc tttttgactg
    13561 gaaaactctg tcaaacattg gcaattgacg gtgtgaccac agcccgcaac gatcgcttgt
    13621 ttgatgctgg cacccatttc catgtccacc tgattccacg ctttcgatca gacggcttct
    13681 gggaccaaat ttcacttgca caagtacagc ttgacctgaa cgcctttctc aaagcattat
    13741 aaaaagcagc ccaacggctg ctttttcgct tatatagtcg tttagtttac ttttagtaca
    13801 aggcaacgag ccgcaggcat aactcaatgc tttcttgttt ctaggagacc agctgatgta
    13861 cttcgctttt ttgttttcag gctcaggtac gaatagtcca ccggactatt cgtatccccc
    13921 agactgtttc aatcaggcaa ggcgagttca aacactccag tggagtgttt gaagttggaa
    13981 ataaggaaac gacgtttcct cgctcgtcga agtaataaaa gagaaactaa atgactatac
    14041 caactcttcc acttcataaa tagtatcttc cagcacctct tggtactgtt ccaagtcata
    14101 ggctacagtg gtttccacct ccgccaactg ctcttccagc tggattttca cctcttccac
    14161 cccatccaac tccaagaagt gattggtcac atgcttgaca cagttttggc acgataagtt
    14221 tttcaatttc aaagtttgct tcatgttttt tctccttttg catgatgccc acaggtacat
    14281 tgccccgcca agcaccgaca agcgatttgc tcgggtgcag tcctttgtaa ctgcgagatt
    14341 ttgctggcca gtttttctag gcttttttga gaaaatatcc ctgtgtctag cagtttttca
    14401 accaattgcc cctgttttgt ggaacatatg gtagctagta agagctccac ctgcccctgc
    14461 aaatgttcct cttcactgat cagcggataa tagtggtatt tgctggtttc cttggccatt
    14521 ctcaaatagt ttttcttccg caaccgcccc aagagtgtct taatggtcgt cgcctgccaa
    14581 tcaaagcctt cctgcaaggc ctgaataatt tcctgagaag tcgccccagg ttgcgcccaa
    14641 agaacccgca taacctgcca ctccgcagct gaaatcgtct gctccacact ctcacctcca
    14701 gccaatcaga tttctaaatt tagtttacac ttgtagtcaa attctgtcaa gaagaaagac
    14761 tgaacatctt gcccagtctt actctcttag tgagccaaca tttcctgacc aatttccaaa
    14821 ccagtcaaac caactggata gtaggttggc caattttcca attcttccaa gagtttttct
    14881 tggctttctc caccgaaata gatatggaag tgacccgttt taacaggtgc aatattgtgg
    14941 tcgctaaatt gaacatactt atagttacca gcatttgcat ccgttgcttc aaacaagaaa
    15001 cgaacaccac gatttccttt tttgtaagtc aaaatcttat agcctacata cttatacgtg
    15061 aatttttctt tcttatctcc cactacaaat tccatggtat tatctgtaat gttaatttga
    15121 tcaacatctg ttttgtagcc agtatcgtaa taagccttgt actcttcagc tgtcatacca
    15181 cctttgatct tagccttgta atcccagacc tgatctagtg taccatcaag caagtatgga
    15241 taaacagatt gccactcacc tgcataatct gaaagagtac gatcttttac ggcactatct
    15301 tcaaaataac cgttataaac agtttgcgca gtctcagcac cttcttctgg caaaatttca
    15361 gaaccttctt gggaagttgt tttttcaagg gcagtcagat tatcttccat aacagaaata
    15421 taatccttac catttttcat atcctcatcc gtcaaacttt caagaggatt aagaacatcc
    15481 aactgaaccc cagtctcttt agcaagtgtt tctgcaacag attttgacgc attttcttca
    15541 aaatagatat atttaatacc atatttatta atatactctg tcaattctgc tagacgtgat
    15601 ggagtcggat cttcatccgc agcaacacct gtaatagaaa cttgtttcaa accatagtcc
    15661 aaagccaaat atgcaaatgc agtatgttgt gtaacaaagt atttctgttt cgcagcagat
    15721 aatgtttcag aatatttagc atccaaagca tctaatttct caatataggc tgccgcattt
    15781 gtttcaaagg catctttctt ttctgggtat ttagctacca aactatcacg aatattttct
    15841 acaagtgtga tggcacgctc aggagacaac catacatgcg gatcataagc atggctatgc
    15901 ctctccccac tatggtcgtg acctttatgt tcctcctcac cgccaggcaa aagcaacatc
    15961 ccctccgtcg cactaatcac attaaccttt gttgtgtcaa gtgatttttc aacatcatga
    16021 acccatgttt ccatattctc attttcataa acaaaagcat ctgcctcttg gatacgagca
    16081 atatctttag ctgacggctc ataaccatga acctctgtcc cagccttaac caacagatca
    16141 acatttgcct catcacctgc cacctgcttg gtgaactcat aaacaggata aaaggtcgtt
    16201 acaatattca acttcccatc ttcagaagct gtgctattgc cacaagcccc caaaagcaaa
    16261 gctgagacag acaagaataa taaaccaact tttttcatct taactcctta ttttttaaat
    16321 ttttgaacaa cattcaccag taagaaaata ctaatgaaaa tcaaggtaat acttgcactc
    16381 gctggggttt ctgcatagta agaagtcagc aaccccatga ccattccaaa aaagccaatg
    16441 cccatcccag taaagataac agctttaaaa ctcttaccaa gtcgcaatgc aatagaagca
    16501 ggcaaaacca taattgttga aaccagcaaa gcacctgccg caggaatcat taaggcaatg
    16561 gcaacaccag tcaccacatt gaaggcaatg gacatagctc gcacaggtaa cccatctaca
    16621 aatgccgtat cctcgtcaaa ggtaagaata tacattggac gtaaaaacag caatgtcagc
    16681 acaatgacaa tcacagcaat cgtaaacaaa gcaattactt gctcctgact aatggtcact
    16741 atcgaaccaa acagatattg ttccagacta agccctgatt tcccaccaga tttattcatg
    16801 acaatgagcg aaatagccaa accagtcgac atgaggatag ccgtcccaat ttccataaaa
    16861 ttcttgtaaa ttgtccgcaa atactctaaa aatacagctg ccacaataac gacaagaaca
    16921 gtcgaaagcg tcggtgaaat ccccaatacc aaaccaaatg caacccccgc caacgaaaca
    16981 tgactaagag tatccgacat caaactctgc cgtctaagta tcaaaaagac ccccaaaatg
    17041 ggcgagaaca gactcatcgc aatgattgcc aggaaagcac gctgcataaa gtcataatga
    17101 aatacagata gatcaaacat gggcaacctc ctcatccgct tcatgaacat tgaaacaacg
    17161 ccaaggagac tgctgatcac gaaccaagtg aatatttcga tcagcatact tattcagctc
    17221 atcaggatca tgagttatca tcaaaaccga ctttccatgc ttcttagccg aatggtgcat
    17281 gagctggtaa aaagcatcct tggtccctgc atccatccct gttgtcggtt catccagaat
    17341 aaaaatatct ggatcagagg caaacatacg cgcaatgacc gcacgctgct tctgcccacc
    17401 actcaaagca cctaagcgct tatcccgatg ttcccacatt ccaactgatt ccaaactaat
    17461 ccgaacatgt tcctcatcat gggctgtcaa acgacgaaac caaccttgtc gaggataacg
    17521 ccctgatttt acaaattcgt aaaccgtgct cggaaaacca gcattaaaac ttgcaatttg
    17581 ctgaggtaaa taggccattc ttagcttctt accctttata ctcttctcag caatagaaac
    17641 cttcccttgt ttgggcttca atatccccaa agtcgcctta atgagggttg tcttggctgc
    17701 gccattctca ccagtcaaag tcacaaattc cccactatca agataataat gaatatgttc
    17761 taaaaccggt tctttgtcgt agtaaaacga caagtcttcc acagtaatat atctcatcta
    17821 ttttctaatc ttctccacta actcacttaa gaatcgagca attgactcct gctcttcaag
    17881 actataatgt tccaaaagct cttcataagc ttccaaggta tgcgcgtgat gatgagcatg
    17941 ctctgtagca atcggcttag cagcctcagt caaactaaag cgaacaatac gagcgtcctt
    18001 atcatctcta accgccacca ataacccctg agcaactaat gactttgtag ccttcgtgat
    18061 cgcagcctga ctaacattca actccttagc aatctcagta ttagtatatg cagccttttc
    18121 aataagcatc aaaatatgct cctgcgtatt cgtcaacttc accgtgcttt gacaagaacc
    18181 aaccaaaatc tccaattgat tctccgaact caaaacaatc tcgtgcaggt acttctcaat
    18241 ctctaataca atacgattca taggcatccc cttaaccttt taattaactt gttaagtata
    18301 ccacatagaa aacaaaaaaa caaggaaaat atttgaaagc taaattccca aagtaagttc
    18361 tagtctgact cgaaaactag aaactagact gagacgaaaa aataaggtag aacttgagaa
    18421 caataaagta cgtttagaat taaagagttt agttgaaaat ggtaaataaa aattactctt
    18481 tacagtccta ttctttacag gtctaagact tagagaagca ctcgcactaa cacggcaaga
    18541 tatcgacctt tcgagcaata caattcatat cacaaagtct gtatacgtta ataaaggcat
    18601 aagccatatc agtgcaactt aaactaaagc aggaactaga agaaatatca tcaataaaaa
    18661 attaagtcaa gaactgcaat attggtaaca gcaacaaaaa catctattag agcagtttac
    18721 aagtgatagc atgagcctac aagtgtttca aagtaatccc attacgatta caaaaattca
    18781 atagaaagca gtacaaaaaa attcttgatc gtgatgcggc tcttaaaaag atacgcatac
    18841 acgattttaa gcactcacac gcttctctat tgataaatcg aggggaagat tatttagttg
    18901 tgaaagaaag gctaggacac gcttctataa caactacaat cgatacctat tctcacttgt
    18961 atccaaacaa gcaaaaagat ttagctgata aattagatga cctaatttaa ggctttatat
    19021 ttacag
//
LOCUS       DBNFEG010000018        18895 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.018, whole genome shotgun sequence.
ACCESSION   DBNFEG010000018 DBNFEG010000000
VERSION     DBNFEG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18895
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.018"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            79..339
                     /locus_tag="AC1PNG_000455"
     CDS             79..339
                     /locus_tag="AC1PNG_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HIP6700990.1"
                     /translation="MNTDKKKMINRLKRAEGQLRGIQKMIEEDQECIDIVTQLSAVRS
                     SINSMMGMVIAQKVQDCITHPSENPEEQEARLAQAINLIIKK"
     gene            350..907
                     /locus_tag="AC1PNG_000456"
     CDS             350..907
                     /locus_tag="AC1PNG_000456"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HIP6700991.1"
                     /translation="MPTEKEKMIAGQFYMAADAELREMRTFARQQMKKFNEELDGQAR
                     SERLKNWLGATGERIYVEPNFYCDYGSNIHVGEDFYANFNCTMLDVCPITIGKNAMLG
                     PNVQLLTPLHPLDPDERNSGLEYGSPITIGDNFWAGGGVTILPGVTLGNNVVVGAGSV
                     VTKSFGDNVVLAGNPARVIKEIPKK"
     gene            966..1808
                     /gene="dprA"
                     /locus_tag="AC1PNG_000457"
     CDS             966..1808
                     /gene="dprA"
                     /locus_tag="AC1PNG_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIP6700992.1"
                     /translation="MNNFELFKWKKAGLTNLNVNKVLAYQEKHGKKLTLRNVAVVSEC
                     KNPVLFMENYKNLDTKACREEFNKYPSFSILDDIYPLALKQIYNPPVLLFYQGDLELL
                     EKSKLAVVGTRNASKNGVQSVQKIIKELNNRFVIVSGLARGIDTAAHIASLKNGGQTI
                     AVIGTGLDKIYPKENRELQTYIGKHHLVLTEYGPGTDPLKYHFPERNRIIAGLVEGLV
                     VCEAKMRSGSLITCERALEEGREVYAIPGSIMDGKSDGCHHLIQEGAKCVTSGLDIIS
                     EYQF"
     gene            1901..3994
                     /gene="topA"
                     /locus_tag="AC1PNG_000458"
     CDS             1901..3994
                     /gene="topA"
                     /locus_tag="AC1PNG_000458"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIP6700993.1"
                     /translation="MATKTVSKKKTTTKKNLVIVESPAKAKTIEKYLGKNYKVVASVG
                     HIRDLKKSSMSIDFDNNYAPEYINIRGKGPLINSLKKEAKNAKQVFLASDPDREGEAI
                     SWHLAHILGLDANDKNRVVFNEITKDAVKNAFQEPRAINLDLVDAQQARRVLDRIVGY
                     SISPILWKKVKKSLSAGRVQSVALKLIIDRENEISNFKPEEYWTIDATFKKGSKKFQA
                     SFYGIDGKKTKIENNEQVKEILARLDGDDFLVEQVERKERKRNAPLPYTTSTMQMDAA
                     NKINFRTRKTMMVAQQLYEGVSLGTGGTQGLITYMRTDSTRISPVAQAQAADFITERF
                     GATYSKHGNKVKNASGAQDAHEAIRPSNVNLTPESIAKYLDKDQLRLYTLIWNRFVAS
                     QMAVAVFDTMSVRLGQNGIVFAANGSQVKFDGYMAVYNDADKNKMLPDMEQGDTVVRA
                     STNPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVKLAAKRFEPT
                     ELGEIVNKLIVEFFPDIVNVKFTADMEQKLDDVEIGKEQWQKVIDGFYQPFKVELSKA
                     EEQMEKIQIKDEPAGFDCDVCGHEMVIKLGRFGKFYACSNFPDCRNTKQITKEIGVTC
                     PVCQKGQVIERKSKRNRLFYGCDRYPECEFTSWDKPVGRSCPKCDHYLVEKKVRGGGK
                     QVVCPNGDYEEDKIK"
     misc_feature    4410..4544
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            4571..5065
                     /gene="rplJ"
                     /locus_tag="AC1PNG_000459"
     CDS             4571..5065
                     /gene="rplJ"
                     /locus_tag="AC1PNG_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIP6700994.1"
                     /translation="MSEAIIAKKAELVNAVAEQMKAATSIVVVDARGLTVEQDTVLRR
                     NLRGAEVEYKVIKNSILRRAAEQAGLEGLAELFVGPSAVAFSNDAVAPAKTIYDFAKT
                     ADALEIKGGAVEGKVSSKEEIEALATLPNREGMLSMLLSVLQAPVRNVAYAVKAVAEK
                     EDAA"
     gene            5131..5496
                     /gene="rplL"
                     /locus_tag="AC1PNG_000460"
     CDS             5131..5496
                     /gene="rplL"
                     /locus_tag="AC1PNG_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIP6700995.1"
                     /translation="MALNIENIIAEIKEATILELNDLVKAIEEEFGVTAAAPVAVAAA
                     GGAAEEAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPAMVKEGVATA
                     EAEEIKAKLEEAGASVTLK"
     gene            5756..5929
                     /locus_tag="AC1PNG_000461"
     CDS             5756..5929
                     /locus_tag="AC1PNG_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700996.1"
                     /translation="MQKNEQSSRQIVMCHLMAIMGIDIEKATWIVGEMEESGLIQFDD
                     MGNVGLLVLEGQS"
     gene            5926..6750
                     /locus_tag="AC1PNG_000462"
     CDS             5926..6750
                     /locus_tag="AC1PNG_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HIP6700997.1"
                     /translation="MKRITANHYQTSERYYKLPKLLFESERYKDMKLEVKVAYAVLKD
                     RLELSLSRGWIDEDGAIYLVYSNSKLMALLGCSKSKLLTIKKTLREYGLIDEVQQSSS
                     EKGRLANKIYLGELEHEPIPVSNSDGGGVQKTLGGYQNQPGPVLNSATSETEVSETDR
                     SETKESESVIEDEEEKESLTSKKCDNDNFQRKVDRVTRYDRDYIWGLVHDQLRQVGLS
                     QAASDYVMIYFDHRYQYALENMRFADRAETIAEYVFNGVLSEWTKVQRLKELKGGE"
     gene            6908..8263
                     /gene="dcm"
                     /locus_tag="AC1PNG_000463"
     CDS             6908..8263
                     /gene="dcm"
                     /locus_tag="AC1PNG_000463"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HIP6700998.1"
                     /translation="MKFLDLFSGIGGFRLGLTRQGHECIGFCEIDKFARKSYKAIYET
                     EGEIEFHDIRQVTDQDFRQLRGQVDIICGGFPCQAFSLAGRRLGFEDTRGTLFFEIAR
                     AAKQIQPRFLFLENVKGLLSHDKGETFRTILITLDELGYDVEWQVLNSKDFQVPQNRE
                     RIFIIGHSRRYRPRFLFPLRGENSSASLERLGNVNPSGKGMNGEVYLSRGLAPTLTRG
                     KGEGTKIAIPVLTPDRLEKRQHGRRFKGNDDPMFTLTSQDRHGVVVAGTLPTSFIQTG
                     RVYDLSGLSPTLTTMQGGDKVPKILCREEALHLKIRKATKLGYAKAIVGDSVNLAYPE
                     SNKRRGRVGKGISNTLTTSDNMGVVVAALEYRKDKWYEVTGIILDGKLYRLRIRRLTP
                     RECFRLQGFPDWAFERAESVSSKSQLYKQAGNSVTVTVIEAIAREFRKIEEEEEHEII
                     T"
     gene            8247..8675
                     /locus_tag="AC1PNG_000464"
     CDS             8247..8675
                     /locus_tag="AC1PNG_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6700999.1"
                     /translation="MKLLHSKSIRNCTELEEAIHQAEVERFSEMIASLPNYDCDIDVT
                     FEDDYHKEMNYPLAYESNLHRIFEFIETQDIKNGVDTYLTDENDLAFRAYGQHYMANG
                     QDGLLTTLITVKSFGEGRSPIDMSKVFTPPTQALEKELSV"
     gene            8685..9068
                     /locus_tag="AC1PNG_000465"
     CDS             8685..9068
                     /locus_tag="AC1PNG_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701000.1"
                     /translation="MLEIYLSRNTSRNQKLLSFCDSHGISYSSKEVSNLSREDLLGLF
                     TKSSDCFTLLSPSLQRFKGHREMKLSELVTVVLRKPDQNLRLPIVVCEDKVYPDMSLE
                     EARTFLPRSQKVVSFREHLFKDLVT"
     gene            9078..9311
                     /locus_tag="AC1PNG_000466"
     CDS             9078..9311
                     /locus_tag="AC1PNG_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIP6701001.1"
                     /translation="MNDRFWENLEIIVMEKGLSWADLARQMFKGQYVYPSEFKRLYQT
                     FRHYKSHRLMPQGKWVEKIVVVLEIDYEDLFRR"
     gene            9314..9895
                     /locus_tag="AC1PNG_000467"
     CDS             9314..9895
                     /locus_tag="AC1PNG_000467"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIP6701002.1"
                     /translation="MKRLFVLYGAIHVNVLLVSLYLVGWLNGAWLPVLQVTFLALLLW
                     GWKRFKIPKRNLSFKERGLWVCGSLGVMVSIALIMSALFSVSEPNQETLVTVQDQIPV
                     LSFILFLLNASVVEEVFYREVLWGVLSQPVIQVLLTSSLFALAHHSSSLFTWVLYGSL
                     GFTLGVVRGQTDCLSSTLVHLSWNGIVFFLSLL"
     gene            9972..10460
                     /locus_tag="AC1PNG_000468"
     CDS             9972..10460
                     /locus_tag="AC1PNG_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701003.1"
                     /translation="MSSEQQERQAIQYAERSAMFTVKVLLKLLEWSARHALAQDSAYK
                     VGVQKLEELLQSPYAIESLNISKDILDKPVDVEKFKELVESENLPIAISWQGDYLHFY
                     AKDKSLLDKHLDELLQKLVSNSEKLKGLTMDKTLDEEIAQAKEQIVMSEPSAVKTKEV
                     IL"
     gene            10460..12280
                     /locus_tag="AC1PNG_000469"
                     /pseudo
     CDS             10460..12280
                     /locus_tag="AC1PNG_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003086094.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            12298..12540
                     /locus_tag="AC1PNG_000470"
     CDS             12298..12540
                     /locus_tag="AC1PNG_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701004.1"
                     /translation="MNQKLTGFVYGVDASSMFAQAMALLQKGMVATGAFLVVMGIINL
                     STNIKDGGPGVRNAILEIVGGVMVGAAGVFITQITI"
     gene            12557..13411
                     /locus_tag="AC1PNG_000471"
     CDS             12557..13411
                     /locus_tag="AC1PNG_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HIP6701005.1"
                     /translation="MNNTLPSAFVFLASEKISSDSLFEGFNVDLESTANLVKSLADYN
                     PTVWSYMSAVTKGVMQPLGVAILAVILILEFSKMAKKIANSGGAMTFEAIAPMIVSYI
                     MVAVVITNTTVIVEAILAVASHIIEGVAGVVSHGGTTYETISGLKGSGIIGKLIVGFF
                     AILIWLVRLVSVMVVNLLITIRFIQLYLMIPFAPLTIPTFLSDDWRSVGIGYLKNIMV
                     YALQGVLIFLIISLVPLFESAGKLALSNGAGVMETLATAFGGLVQAIILIIALVGSQR
                     TARSILGM"
     gene            13474..13827
                     /locus_tag="AC1PNG_000472"
     CDS             13474..13827
                     /locus_tag="AC1PNG_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HIP6701006.1"
                     /translation="MNTRVFKDITKVQHRAWLGFTTRQVIFVLPAIAITILILGLNLF
                     YWQFGDWFVYGLIFTFTIPLMLFGVYRPNDLPFETYLNYRWHYEMTIPDRTLTGQKGI
                     QREKNKSLNETKDLF"
     gene            13784..16135
                     /locus_tag="AC1PNG_000473"
     CDS             13784..16135
                     /locus_tag="AC1PNG_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HIP6701007.1"
                     /translation="MKKTNRSMKPKTSSKYKKTRKPKEEVLPTTVNTLLYQGLFPNGL
                     MQVTPDYFSQSYLLGDVNYQTVGLEDKGAIMETYSDLINSLDDKTNFQLTIFNQRVNL
                     DKFRKSVLYPLHEDGYDTYREELNRIMENNLEAGENNFSAVKLISFGKSDQNPKLAYR
                     SLSQIGEYFKSGFSEIDANFTLLSGESRVNHLADMLRGENHLPFTYQDLVRSGQTTKH
                     FIAPTSLSFKHKNHIEMDDRLLQIVYVRDYGMELGDKFLRELMQSDLEVMISLHAKGA
                     AKSEAMTKLRTKKTLMESQKIGEQQKMARSGVYLEKVSQVLESNIDEADELIKTMTQT
                     GDKLFDTLFVIGVFAEDEDQLKHSLDIIKQVAGSNDLVIDNLTYMQEAAFNSLLPFGK
                     NYLEGVSRSLLTSNIAVNSPWTSVDLQDKGGKFYGINQISSNIITIDRGKLNTPSGLI
                     LGTSGAGKGMATKHEIISTKLKEEESDTEIIIVDPENEYSIIGQAFGGESIDIAPDST
                     TFLNVLDLSDDNMDEDPIKVKSEFLLSWIGKLLDRKMDGREKSLIDRVTRLTYKHFAT
                     PSLVEWVFVLSKQPEQEAKDLALDMELYVEGSLDIFSHRTNIKTDSHFLIYNVKKLGD
                     ELKQIALMVIFDQIWNRVVKNQKLGRKTWIYFDEMQLLLLDKYASDFFFKLWSRVRKY
                     GAIPTGITQNVETLLLDANGRRIIANSEFMILLKQAKSDREELVHLLGLSKELEKYLV
                     NPEKGAGLIKAGSTVVSFRNKIPLQTQLFDIMSTDPEKMRGES"
     gene            16132..>18895
                     /locus_tag="AC1PNG_000474"
     CDS             16132..>18895
                     /locus_tag="AC1PNG_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HIP6701008.1"
                     /translation="MTREQQKVKVARKTFQSNLKASRIHYRREKKGLKRSLPKRRFIM
                     RRAEKAETREQRQALKQTYQEEKDLATDTFKEAIAYVSPRWLKAREIKKYRLPQARQR
                     LAVARKHLAEVKMAEKEEAVTSKVTDQKKPTESKTSRFQFQKEKPLERLQADKEVKSA
                     KRDVKQLKKAHQFKNPSTKVKRGLRYVASESLDVVAQVDDLEGIRTLKESVIKGRRYG
                     RFTYQSGKLLVKSGQTGLRFTKTKAAHSRERYQNFKKGKGFTRQKPLKPKRRYHTFLK
                     QARRHSVSGLRGVIQAIKNSLTFFSSIALNPMTWVVSGLLFFLLLMMSFVIGISGTTL
                     IQQDESELTKAYTHMTWEDAENTRTNPTGITYYTKIDDVMGFMNLKYQDYALEEVMEE
                     GDKTYQAYLSQLWQDLNGGDSLKTMLELSKEPAYKLSDDDREDLKELSEEGTYLALQE
                     LDNPFQDQTEDDALTMTVRYGYEVIDDKPTLHHHIILEAKENQVIVAPMDGKVSLDGE
                     NIIITSGKGLNKSQLTLFNIHTGRVSDGQKVQAGEVIGQTKDGAGLKVTYQKVDDDSE
                     KLVYVNPAFYFPKVIQLQTTILPTIGQFGGDEFARAKAIYEYLKSQGATNQAIAAILG
                     NWSVESSINPKRAEGDYLSPPVGATDSSWDDEGWLSLNGPAIYNGRYPNILRRGLGLG
                     QWTDTADGSRRHTLLLEYTKRKNQKWYDLGLQLDFMLNGDNPYYTKWLKDFFNNSGSP
                     ASLAQVFLIYWEGNSGDKLLERQTRATEWYYQIEKGFSQPNGGTAQSDPKALEAVRGD
                     LYDNSIPGGGDGMGYAYGQCTWGVAARINQLGLKLKGRNGEKIPIISTMGNGQDWVAT
                     AARLGGDTGKLPKAGAIISFAGGGHGSPTEYGHVAFVEKVYPDGSFLISETNVNGNPN
                     YTFRKLS"
BASE COUNT         5817 a         3518 c         4316 g         5244 t
ORIGIN      
        1 ctaaaatata tcaaggaatt caaaagatgt caaggtaaaa aattgacatt tttcacttca
       61 agcagtacta tatagggtat gaatactgat aagaaaaaga tgataaaccg tttgaaacgg
      121 gccgaaggtc aactcagagg cattcagaaa atgattgaag aagatcagga atgtattgat
      181 attgtaactc agttgagtgc tgttcgttcc agcattaata gtatgatggg aatggttatt
      241 gctcagaagg tacaagattg tatcactcat ccatctgaaa accctgaaga acaagaagct
      301 cgtcttgctc aagctatcaa tttaatcata aaaaaatagg agcagaagta tgccaacaga
      361 aaaagaaaag atgatagctg ggcaatttta tatggcagca gatgctgaat tgagagagat
      421 gcgaaccttt gcccgccaac agatgaaaaa atttaacgaa gaactagatg gacaagcccg
      481 ctctgagcga ttgaaaaact ggttgggagc tacaggtgag cggatttacg tggagccaaa
      541 tttctactgt gactacggta gcaatattca tgttggtgag gatttttacg caaattttaa
      601 ctgtacgatg ttggatgttt gtcccattac cattgggaaa aatgccatgc ttggtcccaa
      661 tgtacagttg ctaactccac ttcatccact agatcctgat gaacgaaatt ctggtttgga
      721 atatggctct ccaattacca ttggtgataa tttctgggct ggtggtggtg tgacaatctt
      781 acctggcgtg accttgggaa acaatgtagt tgttggtgct ggttccgttg tgaccaagtc
      841 ttttggtgat aatgtcgttt tggctggcaa tcctgcgcgt gttatcaaag aaatacctaa
      901 aaaataattc ttcttcggga gaattttttc tttgccttgc gaatagacta agtgaggtga
      961 agaaaatgaa taattttgaa ttgtttaaat ggaaaaaagc gggtttgacc aatcttaatg
     1021 tgaacaaagt tttggcctat caagaaaaac acggtaaaaa attgaccttg cgaaatgtcg
     1081 ctgtagtgtc tgaatgtaaa aatccagttc tatttatgga aaattataag aatctagata
     1141 caaaagcttg tcgcgaggaa tttaataagt atccatcatt ttcaatttta gatgacatct
     1201 atcctttggc gctcaaacag atttacaatc cacctgtact tttgttttat caaggggatt
     1261 tagaattgct agaaaaatca aagctggcag ttgttggtac acgaaatgcc agtaaaaatg
     1321 gcgttcagtc tgttcagaag attatcaaag aactcaacaa tcgctttgtc attgtcagtg
     1381 gtttggcaag aggaattgat acagctgccc atattgctag cctgaaaaat ggtggacaaa
     1441 ccattgccgt tattggaaca ggtttggata agatatatcc caaagaaaat agagaattac
     1501 agacctacat cggaaaacat catttggttc tgactgaata tggaccaggt acagaccctc
     1561 tcaagtacca tttcccagaa cgcaatcgca taatagctgg tttggtggaa ggattggtag
     1621 tctgtgaagc taagatgcgg tctggtagcc tcattacctg tgaacgtgcc ttggaagaag
     1681 gaagagaagt ctatgctatt ccaggttcca ttatggacgg aaaatcagat ggttgtcatc
     1741 atctgattca agagggagca aagtgcgtta catccggctt ggacatcatt tcagagtatc
     1801 aattttaaaa caagtcttcc tataggaaaa taaatatctt gacactgact gaaaaatagt
     1861 atattctttg tgaggttaaa aatatttgta aggtataaga atggctacaa aaacagttag
     1921 taagaaaaaa acaacaacca agaaaaatct cgttattgta gagtcgcctg ctaaggcaaa
     1981 aacaatcgaa aaatatcttg gtaaaaatta taaggtcgtt gcttcagtcg ggcatattcg
     2041 agatttgaag aaatctagta tgtctattga ttttgataac aactatgcac ctgagtacat
     2101 caacatacgt ggaaaaggac cactcatcaa ttctttgaaa aaagaagcaa aaaatgctaa
     2161 gcaagtcttt ctcgcgagtg acccggaccg cgaaggagaa gcaatttctt ggcatttggc
     2221 tcacattttg ggattggacg caaacgataa aaaccgtgtt gtctttaatg aaattacaaa
     2281 agatgcagta aaaaatgcct ttcaggagcc acgtgctatt aacctagact tggtagatgc
     2341 ccagcaagct cgtcgcgtct tagaccgtat tgtagggtac tccatatcac ctattttatg
     2401 gaagaaagtc aaaaaaagtc tgtcagcagg acgtgttcaa tcggttgctc tcaaattaat
     2461 aatcgaccgt gaaaatgaaa tcagcaattt caaaccagaa gaatattgga caattgatgc
     2521 aaccttcaaa aaagggagca agaaattcca ggcttcattc tacggtatag atggaaagaa
     2581 aactaaaatt gaaaacaatg agcaggttaa agaaattctc gcccgacttg atggagatga
     2641 tttcttagta gaacaggttg agcgtaagga acgcaaacga aatgcacctc taccttacac
     2701 aacttctact atgcagatgg atgcggctaa taaaatcaat ttccgtactc gtaaaaccat
     2761 gatggttgcc caacaacttt atgagggtgt cagtcttggc acaggtggta cccaaggttt
     2821 gattacctat atgcgtacgg attcaacacg tattagccct gttgcacaag cacaagctgc
     2881 tgactttatc acggagcgtt ttggtgctac ctattcaaag catggtaata aagtaaaaaa
     2941 tgccagtggt gcacaagatg cccacgaagc cattcgtcca tcaaatgtta acttaacacc
     3001 tgaatcaatt gcaaaatatc tggacaagga ccagttgcga ctatatacac tgatttggaa
     3061 tcgttttgtc gcaagtcaga tggctgtagc agtttttgat accatgagtg ttcgtttggg
     3121 tcaaaatggt attgtctttg ctgctaatgg tagccaagta aaattcgatg gttatatggc
     3181 tgtctataac gacgctgata aaaataaaat gttgccagat atggaacaag gtgacacggt
     3241 ggtgcgcgcg tcaacaaatc cagaacaaca ctttactcag ccaccagctc gctactctga
     3301 agcaacctta attaaaacat tagaagagaa tggtgtaggt cgcccgtcaa cctatgcacc
     3361 aacgattgaa accattcaaa aacgctatta tgttaagctt gcggctaagc gatttgaacc
     3421 aactgaactc ggagaaatcg ttaataagct gattgttgaa tttttcccag atattgtcaa
     3481 tgtcaaattt acagccgata tggagcaaaa attggatgat gtagaaatcg ggaaagagca
     3541 gtggcagaag gtcattgacg gtttctatca gccattcaag gtagaattat ctaaggcaga
     3601 agaacagatg gaaaaaatcc aaattaagga tgaaccagct ggctttgact gtgatgtttg
     3661 tggacatgaa atggttatta aattaggtcg ctttggaaaa ttctatgcat gcagcaattt
     3721 cccagactgt cgcaatacca agcaaatcac taaagaaatc ggtgtgactt gtccagtttg
     3781 ccaaaaaggg caagtgattg aacgcaagag taaacgcaat cgtcttttct atggttgcga
     3841 tcgctaccca gagtgtgaat ttacttcttg ggacaagcca gtcgggcgtt cttgtccgaa
     3901 atgcgaccac tatctcgttg agaaaaaagt tcgtggtggt ggtaaacaag ttgtctgtcc
     3961 aaatggtgac tacgaagaag acaagattaa ataagattaa actgctattc tcaagggaat
     4021 ggcagttgtt ttttagagtc atgtttagtt tatcttagct tacttcgaca agcgaggaaa
     4081 cttcgtctcc ttattttcaa ctttaaacga ggctggtcaa aaagtccaaa gtcacttctc
     4141 agtgcagtgg ttgattgggt ttaacaggcc agtggagtga aactgttcag tgaactgttt
     4201 cagcctgagc ttagaaatga aaaaatcgag gtaaaaaggt tggagataag atttgcgaag
     4261 caaatcacag caatttgtgt ttcacattcc aagtctgacc tctacaacta aaacgaacta
     4321 aacggcaata ctgcttgaaa aaatgtttaa aaaattaaaa aaataggaga aaagtcttgc
     4381 attattgaaa atactgtgtt atgataattg aggtttgaaa aaactgacct aagacagtag
     4441 gggagctgga ctcataaaca tcctaccgag gcacaaaacg attattgaga taaacgtatt
     4501 tgtactttcg tgtctaggtt taggtgcgat ttttttgtgc ctagcccaaa ataaaaacgg
     4561 aggtaaaaaa atgagtgaag ctattatcgc taaaaaagcg gaattagtaa atgccgttgc
     4621 tgagcaaatg aaagctgcaa catctatcgt tgttgtagac gctcgtggtt tgacagtaga
     4681 acaagataca gttcttcgtc gtaacttgcg tggtgcagaa gttgagtaca aagttatcaa
     4741 aaactcaatc ttgcgtcgtg ctgctgagca agctggtctt gaaggtcttg cagaattgtt
     4801 tgtaggtcca tctgcagttg cattctcaaa cgatgcagtt gctccagcta aaacaattta
     4861 cgatttcgcg aaaactgctg atgctcttga aatcaaaggt ggtgctgtag aaggtaaagt
     4921 ttcttctaaa gaagaaatcg aagcacttgc tacattgcca aaccgcgaag gcatgctttc
     4981 tatgttgctt tctgtacttc aagcgccagt tcgcaacgtt gcatacgctg tcaaagctgt
     5041 tgcagaaaaa gaagacgcag cttaatgcgt cagagtgtag ctgcttagtg ctacaaacta
     5101 tttaaaaacc taaattggag gaaaatcaca atggcattga acattgaaaa cattattgct
     5161 gaaattaaag aagctactat ccttgagctt aacgaccttg ttaaagctat cgaagaagaa
     5221 tttggtgtaa ctgcggctgc tcctgtagct gttgctgcag ctggtggtgc tgctgaagaa
     5281 gctaaagatt cattcgacgt tgaattgaca tctgctggcg acaaaaaagt tggcgttatc
     5341 aaagttgtac gtgaaatcac tggtcttggt cttaaagaag ctaaagagct tgttgacggt
     5401 gcaccagcta tggttaaaga aggcgttgca actgcagaag ctgaagaaat caaagctaaa
     5461 ttggaagaag caggcgcttc agttactctt aaataagaag cacatagcaa tttgagagcg
     5521 gaagcccgat ttaccagtct tttagagctt atagcgattt aaagaaactg ataatttgat
     5581 ttggtttcct gccaaaaatc ctgccaaaaa atctttggca ggatttttgt ttttatataa
     5641 atatactgtg cagaattgct attcttcaac atttgtattt gtagaaatat atttttatgt
     5701 gagcgagaac atttcaataa gtgttctcgc tttttttatt ttcaggagga aaatcatgca
     5761 aaagaatgaa cagtcatcac gacaaattgt gatgtgtcat ctcatggcta tcatgggaat
     5821 cgatattgag aaagcgacat ggatagttgg tgagatggaa gagtctggct taatccagtt
     5881 tgatgacatg gggaatgttg gtctgttagt gctggaggga caatcatgaa acgtatcacc
     5941 gcaaatcact atcaaacgtc agagcgttat tataagctcc ctaaactctt atttgagagc
     6001 gaacgctaca aggatatgaa actagaagtt aaggttgctt atgctgttct aaaagaccgt
     6061 ttggaattgt cattaagtag agggtggata gatgaagatg gagctattta tttggtctat
     6121 tccaactcca aactcatggc actacttggc tgttccaagt caaaattatt gacgattaag
     6181 aaaaccttac gagagtatgg tttgatagat gaagtccaac aatcttccag tgaaaaagga
     6241 cgcttggcca ataagattta cttgggggag ttggaacatg aacctatccc agtctcaaat
     6301 tcagacgggg gtggtgttca aaaaacacta ggggggtatc aaaatcagcc ggggccagtc
     6361 ttaaattcag ccactagtga gactgaagtt agtgagactg ataggagtga aactaaagag
     6421 agtgagtcag tcattgagga cgaggaggag aaagaaagtc taacaagtaa aaaatgtgac
     6481 aatgacaatt ttcaacgtaa agtagaccga gttacaagat acgacagaga ttacatttgg
     6541 ggcttggtac atgatcagtt gagacaagtc ggtctatcac aggctgctag tgattatgtc
     6601 atgatttatt ttgatcatcg atatcagtac gctttagaga atatgaggtt tgcggacagg
     6661 gcagaaacga tagcggaata cgtctttaat ggcgtcctat ccgaatggac caaggtccaa
     6721 cgcttaaaag aactaaaagg gggtgagtaa gatgttttgg tggatattat taggtatttg
     6781 gggattagga atgatttggt tcatttttga acttattact gccccagaaa tggaagatca
     6841 ttgttaagca aggacgttac gcaagaagta aggtcctttt tagttttgtc agaaaggaat
     6901 gagagacatg aaatttttag atttgttttc aggaattgga ggctttcgac tggggttaac
     6961 ccgtcagggc catgagtgta ttggcttttg tgagattgat aagtttgcga ggaaatctta
     7021 caaggccatc tatgagaccg aaggagaaat agaatttcat gatattagac aagtcacaga
     7081 ccaagacttt aggcaactta gagggcaagt ggacatcatc tgtgggggat tcccttgcca
     7141 ggcattttca ctcgcaggca gacgattggg atttgaggat actaggggaa ctttgttctt
     7201 tgagattgct cgagcggcca aacagatcca accacgtttt ttatttctcg aaaatgtcaa
     7261 gggcttactc agtcacgaca agggagagac atttcgaaca atcctcatca cattggatga
     7321 gttggggtat gatgttgaat ggcaggtgct taacagtaaa gatttccaag tgccgcaaaa
     7381 ccgtgaaaga atttttatta tcggacattc tagaagatac cgtccccgat tcctatttcc
     7441 tctcagagga gaaaacagct cagctagtct tgaacgatta ggcaatgtta atccgtcagg
     7501 caaaggcatg aatggggaag tctatttatc acgtgggcta gcaccaacct taaccagagg
     7561 aaaaggtgag ggaactaaaa ttgccattcc tgtcttaacc cctgataggc tagaaaaacg
     7621 ccaacatggg agacgcttta aagggaacga tgatccgatg tttactttaa ctagccaaga
     7681 tcggcatggt gtagttgtcg caggaacttt accaacttct tttatccaaa ctggacgtgt
     7741 ctatgacctt tcagggcttt ctccaacgtt gacaaccatg caaggagggg ataaagttcc
     7801 taagattctc tgtcgtgaag aagcgctaca tttgaaaatt aggaaagcga ctaaactagg
     7861 gtatgccaag gcaatagtgg gagattctgt caaccttgct tacccagagt ctaacaaacg
     7921 cagaggacga gtgggaaagg ggatttcaaa tactttaacc acttctgaca atatgggagt
     7981 ggttgttgct gctttggagt accgtaagga caagtggtat gaagtgactg gaataatcct
     8041 agatggaaaa ctgtatcgtt tgagaatcag acgcctaacc ccaagagagt gttttaggtt
     8101 acaaggattt cctgactggg cttttgagag ggcagaaagt gtttcaagta aaagtcagtt
     8161 gtataaacaa gcaggcaata gcgtgaccgt cactgttatc gaggccattg ctagagaatt
     8221 tagaaagatt gaagaggaag aagaacatga aattattaca tagtaaaagt attaggaact
     8281 gcacggagct agaagaagcc atacatcaag cagaagtgga gagattttcg gaaatgattg
     8341 cttctttacc caattatgat tgtgacattg atgtcacttt tgaagatgac taccacaaag
     8401 aaatgaatta tccgttagct tatgagtcca acttacaccg tatttttgag tttattgaaa
     8461 cccaagacat caaaaatggt gttgatactt atctaacgga tgagaacgat ctcgccttta
     8521 gggcatatgg tcaacattat atggcaaatg gacaagatgg tttattgact acactgatta
     8581 cagtaaagag ttttggtgaa ggcaggtcgc ctattgatat gagtaaggtg tttaccccac
     8641 caactcaagc attagagaag gagctatctg tgtaggagga tacgatgtta gaaatctatt
     8701 taagtcgaaa taccagtcgg aatcaaaaac tcctttcttt ttgtgactcg catggtattt
     8761 cctactccag taaagaagta agtaacctgt cacgtgaaga cttattgggc ttgtttacca
     8821 aaagcagtga ttgttttacg ctcttatccc cctcgctaca acgatttaaa ggtcatagag
     8881 agatgaaatt gagtgagtta gtgacggtgg ttctacggaa acctgaccaa aatcttcgtc
     8941 tcccaattgt ggtttgtgag gacaaagtgt atcccgatat gagtttagaa gaagcaagga
     9001 cctttcttcc gagaagtcaa aaagtggttt cctttcgtga acatcttttt aaggatctgg
     9061 tgacataagg aggagttatg aacgatcgtt tttgggaaaa tttggaaatt atcgttatgg
     9121 aaaaaggact gtcatgggct gaccttgctc gacagatgtt taagggacaa tacgtttatc
     9181 ctagtgagtt taagcgcctc taccaaacct ttcgtcacta caaatcccat cgtctcatgc
     9241 cacaaggtaa atgggtggag aaaatagtgg ttgttttaga gattgactat gaagacttgt
     9301 ttaggagata gcgatgaaac gactatttgt gttgtacgga gccattcatg tcaatgtact
     9361 cctagtgagt ttgtacctag ttggttggct aaatggggca tggttacctg ttttacaagt
     9421 gacattttta gccctgctat tgtggggttg gaaaaggttt aagataccga aaagaaacct
     9481 atcctttaag gagagagggc tttgggtgtg tggtagtcta ggtgtcatgg taagtattgc
     9541 tcttattatg agtgctctat tttcagtaag cgaaccaaac caagaaacgt tagtgactgt
     9601 tcaagaccag attcctgttc tgtcctttat cctttttctc cttaatgcca gtgttgtgga
     9661 agaagtcttt tatagagagg tgttgtgggg agtcttgtct caaccagtga ttcaagtgtt
     9721 gttaacgagt tccctctttg ccttagctca tcactcatcc agtctattca cttgggtgct
     9781 ttatgggagt ttaggtttca cacttggtgt ggttagaggg caaacagact gcttgagctc
     9841 aactctcgtt cacctgtctt ggaatggaat cgtctttttc ctatctttat tgtgatacca
     9901 aaatcaaggg tgctttcgta tcatgaaagc accctattgt tttgatgggg aatcatataa
     9961 aggaggaaat gatgtcatcc gaacaacagg aacgccaagc gattcaatat gcagaacgga
    10021 gtgctatgtt tacggtgaag gtccttctca aactcttaga gtggtcagcc agacacgccc
    10081 tagcacaaga ctcggcttat aaagttgggg ttcaaaagtt agaagaactc cttcaaagtc
    10141 catatgccat tgagtcgctg aatatctcaa aagacatttt ggataaacca gttgatgtgg
    10201 agaaattcaa ggaattggtg gaatcagaaa accttcctat tgccattagt tggcaaggag
    10261 attacctgca tttctatgct aaggataagt cgctcttaga caagcactta gatgagttgc
    10321 ttcagaagtt ggtttccaat tcagaaaaac tgaaagggct aactatggat aaaacccttg
    10381 atgaggaaat cgcacaagcc aaggaacaga ttgtgatgtc agaaccctct gctgtgaaaa
    10441 caaaggaggt cattctctaa tggtcttagg gaaaaaagtt tttatctttg gtattttagg
    10501 gcttgctctt ggctatttct gccatcggct agtcttgctt tacgatagtc tacctaatca
    10561 accacccttg gaacgccttg cctatctctt aggagaggga cagaatcagg tcttaaatcc
    10621 cttgtggaat ggcaacttta caggtaaatc agttttgggc ttttgctttg gatttgtgac
    10681 gatgggccta gtctatcttt atgtttcaac tggtcaaaag gtttaccgag aaggggcgga
    10741 gtatggctct gcccgttttg gaaacagtca cgaacgcaaa gcctttctca gtaagaatcc
    10801 tttcaatgac actattttgt cacgaaacgt taggctaacc ctgttagaaa agaaagccct
    10861 acagtttgac cgaaacaaga accttgtggt cattggtggg tcaggggcgg gaaagacctt
    10921 tcgctttgtg aaacccaatc tgattcagct caactgttcc aatattgtcg ttgaccctaa
    10981 ggatcactta gctgaaaaga cagggaagct ctttttagag catggctatc aggtcaaggt
    11041 cttagacctt gtaaacatga caaactcaga tggctttaat ccttttcgtt atgtggaaac
    11101 ggagaatgat ttgaaccgta tgcttacggt ctatttcaat aataccaaag gcaatggcag
    11161 tcgcagtgat cccttttggg atgaggcgtc catgacctta gtccgtgcta tttcctctta
    11221 cttggtggat ttttacaatc ctccaggcag taccaaagaa gaagcagaca gtcgccgtaa
    11281 gcgaggccgc tacccctctt tttctgaaat tggaaaactc atcaagctct tatccaaagg
    11341 agaaaatcaa gataagagtg tcttagaagt gatgtttgag acctatgcca agacatatgg
    11401 gacagaaaac ttcaccatgc gtaactggtc agacttccaa aactacaagg ataagacctt
    11461 ggattcagta attgccgtaa caacagctaa gttcgccctc tttaatatcc aatccgtcat
    11521 tgatttaacg aaacgtgaca ccttggactt aaagacatgg ggaacgcaga aaaccatggt
    11581 ctatctcgtt attccagata atgacaccac ctttcgcttt ctctcagctc tctttttctc
    11641 aaccgtcttt tcaaccttaa ctagacaagc tgatgtggac tttaaggggt aactgcctat
    11701 ccacgtcaga agttatttag atgaatttgc gaatgtcggc gaaattccag actttgcgga
    11761 acaaacctca acggttcgct ccagaaacat gagtctcgta cccattcttc aaaacattgc
    11821 ccaacttcaa gggctttata aggagaagga ggcttggaaa accattctag gaaactgtga
    11881 cagtttgctt tatcttggag gaaatgatga agagaccttt aagtttatga gtggtctctt
    11941 aggcaaacaa accattgatg tgagaagtac cagtcggtcg tatggccaaa cagggtctgg
    12001 gtcaacttcc catcagaaaa tcgctaggga tctcatgacc cctgatgaag tcggaaacat
    12061 gaaacgtgat gagtgcttgg tgagaattgc aggggttcca gtctttaagg aaaagaagta
    12121 ttttcctttg aaacataaga actggcaata cttagcggat aaggagtcag atgaacgttg
    12181 gtggcattac cacattgacc ccctcaaaac agaggaagtt ccgtttgaac catcagacca
    12241 caaggttagg gatttaagta cagaaagcac actacactaa tagaaaagag gaatcgtatg
    12301 aaccaaaaat taacaggctt tgtttatggc gtggacgcta gttccatgtt tgcacaagcc
    12361 atggcgctct tacaaaaagg aatggttgcg acaggagcct ttcttgtcgt catgggtatt
    12421 ataaacctat caaccaatat caaagatggt ggacctggtg tccgcaatgc catcttagaa
    12481 attgtgggtg gtgttatggt aggtgccgcc ggtgtcttca tcacccaaat taccatttag
    12541 gagggctaag tatgacatga acaacacatt accttcagct tttgtctttc tagcgtcaga
    12601 gaaaatttcg agcgatagcc tttttgaagg ctttaacgtg gacttagaat cgacggccaa
    12661 cttggtcaaa tcccttgcgg attataaccc aaccgtgtgg tcttatatgt cagctgtcac
    12721 aaaaggagtg atgcagccct tgggagtggc tattttagca gtcattctca ttcttgagtt
    12781 ttctaagatg gctaagaaaa tcgccaactc aggtggtgct atgacctttg aagctattgc
    12841 tcctatgatt gtctcttata ttatggtggc tgtggtcatt acaaacacca ccgttattgt
    12901 agaagccatc ttagccgttg cttctcacat cattgaaggc gtagctggtg tcgtttctca
    12961 tgggggaacg acttatgaga ccatttctgg acttaaaggg tcaggaatta ttggaaaact
    13021 cattgtagga ttttttgcca ttctgatatg gttggtccgt ttggtgagtg tgatggtggt
    13081 gaatctcctc attaccattc gctttatcca actctatctc atgattcctt ttgcaccttt
    13141 aacgattccg accttcctca gcgatgattg gcgaagcgtt gggattggct acctgaaaaa
    13201 tatcatggtc tatgcccttc aaggggtctt gattttctta atcatctccc ttgttcccct
    13261 ctttgaatca gctgggaaat tggctctctc aaatggtgca ggagtgatgg agacgctagc
    13321 gacagccttt ggtggtttag ttcaggctat tatattgatt attgccttgg taggcagtca
    13381 acggactgcc agaagtatcc taggcatgta aggaggattg ggaaggacgt tcctcccaat
    13441 ctttttttca tattcatctt agattggagt cttatgaata cacgtgtttt taaggacatc
    13501 acaaaagtcc aacatagggc ttggctaggc tttaccacac gacaagttat ctttgtcttg
    13561 ccagccattg ccattaccat cctgatttta gggctaaacc tcttttattg gcaatttggg
    13621 gattggtttg tctatggtct tatctttacc tttaccattc ctcttatgct ttttggggtc
    13681 tatcgcccta atgacttacc ttttgaaacg tatcttaatt atagatggca ttatgaaatg
    13741 accataccag accgtacact aactggacag aaaggaatac aacgtgaaaa aaacaaatcg
    13801 ctcaatgaaa ccaaagacct cttctaagta caagaagaca cgtaaaccaa aagaagaagt
    13861 gctaccaaca actgttaata cgcttcttta ccaaggcctt tttcctaatg gtctcatgca
    13921 ggttacccca gattattttt cccagtccta tcttttaggg gatgtcaatt accaaacggt
    13981 gggacttgaa gataagggag cgattatgga aacctattca gacttgatta acagtttgga
    14041 tgacaagacc aatttccaac tcactatttt taaccaaagg gtcaacttgg ataaatttag
    14101 aaaaagtgtc ttatatccct tacatgaaga tggctacgat acttatcgtg aagaactcaa
    14161 tcgtatcatg gagaataact tagaagctgg agaaaataac ttttcagcgg ttaagctcat
    14221 ttcttttggg aagtcagacc aaaatccgaa actcgcttat cgctccctgt ctcagattgg
    14281 ggaatatttc aagagtggtt tttctgaaat agatgcgaat tttactctcc tcagtggaga
    14341 aagccgtgtg aaccacttgg ctgatatgtt gcgaggggaa aatcatttgc cctttactta
    14401 tcaagacctg gtgcgttcag gacaaacaac caagcacttt attgccccaa ccagtctgtc
    14461 ctttaagcac aaaaatcata ttgaaatgga tgatagactg cttcagattg tctatgttcg
    14521 tgactatggg atggagttgg gagataagtt cttacgagaa ctcatgcagt cggatttaga
    14581 agtgatgatt agtcttcatg ccaaaggagc agccaagtca gaagccatga ccaagctccg
    14641 caccaagaag actttgatgg aatcgcaaaa aattggggag caacaaaaga tggcacgatc
    14701 tggcgtttat cttgaaaagg ttagtcaggt cctagaaagt aatattgatg aagccgatga
    14761 gttgattaaa accatgacgc aaacaggcga caagctcttt gacacccttt ttgtgattgg
    14821 ggtctttgcg gaggacgaag accagttgaa gcactccctt gatattatca aacaagtggc
    14881 aggttctaat gaccttgtga ttgataatct gacctatatg caagaagctg cctttaatag
    14941 cttattgcca tttggcaaga attaccttga gggggtgtca cggtctcttt tgacctcaaa
    15001 cattgccgtg aattccccct ggacatctgt agaccttcaa gataagggag ggaaatttta
    15061 tgggataaac cagatttcaa gtaatatcat caccattgac cgtgggaaac tcaatacccc
    15121 atcaggattg attctaggaa cttcaggtgc aggtaaggga atggcgacta agcatgaaat
    15181 catctcaacc aagttaaaag aagaagagag tgataccgaa atcatcattg tagaccctga
    15241 aaatgagtac agtatcattg gtcaagcctt tggtggggaa agcattgaca ttgcgcctga
    15301 ctctaccacc ttcttaaatg tcttagactt atctgatgac aacatggatg aagacccaat
    15361 taaggtcaaa tctgaatttc tactttcttg gattgggaaa ctcttagacc ggaaaatgga
    15421 tggtcgagaa aagtccctaa ttgaccgtgt gacacggtta acctataagc attttgccac
    15481 accatctctc gtggaatggg tctttgttct ctctaaacaa cctgaacaag aagccaaaga
    15541 cttagccctt gatatggagc tttatgtgga agggtcactc gatattttct cacaccgaac
    15601 caacatcaaa actgacagcc atttcctcat ttacaatgtt aaaaagttag gggatgagtt
    15661 gaagcagatt gctctcatgg tcatctttga ccagatttgg aatcgtgtgg ttaaaaacca
    15721 aaaactgggg aggaagactt ggatttactt tgatgaaatg cagcttctgt tgttggataa
    15781 gtacgcttct gatttcttct ttaaattatg gagtcgtgtc cgtaagtatg gggccattcc
    15841 aacaggcatt actcaaaacg tagaaacctt acttcttgat gccaacggga gacgtattat
    15901 cgctaatagc gagttcatga tacttctaaa acaagctaaa agtgacagag aagagttggt
    15961 ccatctcttg ggcttatcta aggagcttga gaaatatttg gttaacccag aaaagggagc
    16021 tggattgatt aaggcaggct caacggttgt ttcctttaga aataaaatcc cacttcaaac
    16081 ccaactcttt gacatcatga gtaccgatcc agaaaagatg aggggtgaat catgacaaga
    16141 gagcaacaga aggtcaaggt tgcccgtaaa acctttcaga gtaatttaaa ggccagccgt
    16201 atccattatc gcagggagaa gaaggggtta aaacgctcac ttcctaagcg acgctttatc
    16261 atgcgccgag ctgagaaagc tgaaacaagg gaacaacgtc aggctctgaa gcaaacctat
    16321 caggaggaga aggacttagc aacagatacc tttaaggaag cgattgccta tgtgtctccc
    16381 agatggctaa aggctaggga aattaagaag tatcggcttc ctcaagccag acagcgccta
    16441 gcagtagcaa gaaaacactt ggcagaggtc aagatggccg aaaaagagga ggctgttacc
    16501 tctaaggtta ccgatcagaa aaagccaaca gaatcaaaaa catcacggtt tcagtttcaa
    16561 aaagaaaaac ctcttgaacg ccttcaggca gacaaagagg tcaaatcagc gaagcgtgac
    16621 gtgaaacaac tgaaaaaagc acatcagttt aagaacccct cgactaaggt caaacgaggg
    16681 ttacgctatg tcgcttcaga atcacttgac gtagtcgctc aagttgatga cttggaggga
    16741 atcagaaccc taaaagagtc tgtgattaaa ggaagacgct atgggagatt tacttatcag
    16801 tcagggaaac tccttgtcaa aagtgggcag actggtcttc ggtttactaa gacgaaagcc
    16861 gctcatagta gggagcggta tcagaacttt aaaaagggta aagggttcac ccgacagaag
    16921 cctttaaagc ccaaaaggcg atatcacacc tttctcaagc aagccagaag gcacagtgtg
    16981 tcaggactca gaggggttat tcaagcgatt aagaatagcc tgaccttctt ttcatcgatt
    17041 gcccttaatc ctatgacttg ggtcgtgtca ggtctcttgt ttttcttgct tttgatgatg
    17101 agttttgtga taggaatttc aggaactact ctcattcaac aggatgagag tgaactgaca
    17161 aaagcctata cgcacatgac gtgggaagat gcggaaaaca cccgaaccaa tccaactggc
    17221 attacctact acaccaagat tgatgacgtc atgggcttta tgaatctcaa gtatcaagat
    17281 tatgctttag aagaagtcat ggaagaaggg gataaaactt atcaagccta tctcagtcag
    17341 ctttggcagg acttaaacgg tggagattct ttaaaaacca tgcttgagtt aagtaaggaa
    17401 cctgcctata agctctctga tgacgataga gaagacttaa aggaattaag tgaggaaggc
    17461 acctaccttg ccctccaaga attggacaat ccttttcaag atcagactga agacgatgcg
    17521 cttaccatga cagtacgcta tggctatgag gtgattgatg acaagccaac gcttcatcat
    17581 catatcattc tagaagctaa agaaaatcaa gtgattgtgg ctcccatgga tggtaaggtt
    17641 tccctagatg gcgagaacat tatcatcacg tcaggtaagg gactaaacaa gtctcaattg
    17701 accttgttta atatccatac tggtcgagtg agcgatggcc aaaaggtaca agcaggagag
    17761 gtgattggtc aaacaaagga tggtgctggt cttaaagtga cctatcaaaa ggttgacgat
    17821 gattcagaga aactggttta cgtcaatcca gccttttact ttcctaaagt cattcaactc
    17881 caaacgacca ttctacctac cattggtcaa tttggaggcg atgagtttgc gagagccaaa
    17941 gctatctatg agtatttgaa gagtcaaggg gcgaccaatc aagccattgc ggctatttta
    18001 ggaaactggt cagtagaatc ttcaatcaat cccaaacgag ctgaaggtga ctacttatcg
    18061 cctcctgttg gggcaacgga tagttcttgg gacgatgagg gatggttgtc tctaaatggt
    18121 ccagctatct ataatggcag gtacccgaat attttaagac gtggcttagg cttggggcag
    18181 tggacagata cagcagatgg gtcacgcaga cataccttat tattagagta taccaaacga
    18241 aaaaaccaga agtggtatga cttaggctta caactggatt ttatgctcaa tggggacaat
    18301 ccttactata ccaaatggtt aaaagacttt ttcaataatt caggtagccc agccagtctt
    18361 gcccaagtct ttctcattta ttgggaggga aatagtggtg ataagctcct agaacgtcaa
    18421 acgagagcta cagagtggta ttaccaaatt gagaaaggct ttagccaacc aaatggtgga
    18481 accgctcaga gtgatccaaa agcacttgag gctgttcgag gagatcttta tgacaactct
    18541 attccaggtg gaggtgacgg tatgggttat gcctatgggc aatgtacgtg gggagtggcc
    18601 gctcgtatca atcaattggg cttgaaactc aaaggacgaa atggtgagaa aatcccaatc
    18661 attagtacca tggggaatgg tcaagattgg gtggcaacag cagcacgtct tggtggagat
    18721 acaggtaagc taccaaaagc aggagcgatt atttcttttg cgggaggagg tcatggatcg
    18781 ccaacagaat acggtcatgt cgcctttgtc gagaaggttt accctgatgg ttctttcctc
    18841 atctcagaga ctaatgttaa tggtaatcct aactatacct tccgtaagtt atcag
//
LOCUS       DBNFEG010000019        18884 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.019, whole genome shotgun sequence.
ACCESSION   DBNFEG010000019 DBNFEG010000000
VERSION     DBNFEG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18884
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.019"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            225..1886
                     /locus_tag="AC1PNG_000475"
     CDS             225..1886
                     /locus_tag="AC1PNG_000475"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIP6701009.1"
                     /translation="MSTIKIMALGGVRENGKNLYIAEVNEHIFVLDAGAKYPENEQLG
                     VDVVVPNFDYLEENKHRVAGVFLSHGHADAIGALPYLLEKVKVPVFGSHLTIELAKLL
                     VKGNNATKKFNDFHVINAETEIDFGDSVVSFFQTTHSIPESLGIVVKTDEGNIVYTGD
                     FKFDQAADKFYRTDFGRLAEIGNEGVLALLSDSANADSNVQVASMHEAGEEILNTIAD
                     CDGRVIVAAVASNIVRIQQIFDAAEATGRRVVLTGHDVENIVRTAIQLKKLRLVSERL
                     LVKPKDMNKYEDHELIILETGRMGEPLNGLRKMSIGRHRYVEIKEGDLVYIVTTPSIS
                     KEAVVARVENLIYKAGGTVKSITKNLRVSGHGNARDLQLMLNIMKPKYLFPVQGEYRQ
                     LDAHAKAALEIGMYPENIIIVKRGDVMAFEEGDFVHSGAVPAGDVMIDGNAMGDVGNI
                     VLRDRKILSEDGIFIVAITVNRREKKIISKAKVNTRGFVYVKKSKDILREASELVNTT
                     VENYFTKDSFDWAELKSAVRDDLAKFLFDQTKRRPAILPVIMEVK"
     gene            1951..2727
                     /locus_tag="AC1PNG_000476"
     CDS             1951..2727
                     /locus_tag="AC1PNG_000476"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIP6701010.1"
                     /translation="MAIMKIEYHSEALDMSRQVTVLYPDRNRVENPDDNDIPVLYLLH
                     GMGGNQDSWLNRSTVERLVRYTNLIVVMVNTDKGWYTNTTYGMNYFDAIAVELPQILK
                     RFFPNMSDKREKNFIAGLSMGGYGTFKIAMMTNRFSHAASLSGALYFDFDNPAAAELG
                     SLSYWQGVFGDISDKQNPNNLLEIAKQSDKKTKFYAWCGEEDFLFEGHQKAVGELKEL
                     GFDIEASFGPGKHEWYYWNQQIEKVLAWLPIDFKLEERLS"
     gene            2793..3488
                     /locus_tag="AC1PNG_000477"
     CDS             2793..3488
                     /locus_tag="AC1PNG_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HIP6701011.1"
                     /translation="MKLIGNILRWIWRTFWGLIWLSMMLLACGLGLLFYFQQEAAPQM
                     LQTLAQEVQLMVKGQSEVTIEEGVNTIKHLTTDTVNTADHGRWESNTATVYIETQNPT
                     FVAAYETAIANWNTTGAFTFVMTSDPNQADIIATEMNDGNTQAAGEANSTTNLLTNYY
                     SSVTVRLNSFYLLNDQYGYDMDRIIHTAEHELGHAIGLDHEDSQTSVMESAGSNHGIQ
                     QADIDAVLALYSE"
     gene            3499..3864
                     /locus_tag="AC1PNG_000478"
     CDS             3499..3864
                     /locus_tag="AC1PNG_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701012.1"
                     /translation="MLRTITNTIKRYPEQALLFLYNAGIFAWMQSTSHSIMEQIGIDS
                     NWFDKIPEPIKAWTGASLESMQTLLNSSAWGWLIVSMILMLVIRFVKGLIKFVIMLII
                     IGGGLYLLWQNKELVQSLV"
     gene            3915..5813
                     /locus_tag="AC1PNG_000479"
     CDS             3915..5813
                     /locus_tag="AC1PNG_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIP6701013.1"
                     /translation="MIILSGNKIERSFAGEVLFNNINIQVDERDRIALVGKNGAGKST
                     LLKILVGEETATSGEISTKRDLSLSYLAQDSRFESANTIYDEMLHVFDDLRTTEKRLR
                     SMEEQMGSLSGSDLDQLMKTYDSLSEEFRLAGGFSYEADIRAILNGFKFDQTMWDMKI
                     SELSGGQNTRLALAKMLLESPELLVLDEPTNHLDIDTIAWLENYLVHYKGALIIVSHD
                     RYFLDKVATLTLDLTKHSLDRYVGNYSQFVELKEQKLQTELQNYEKQQKEIAKLEDFV
                     QKNIVRASTTKRAQARRKQLEKMERLDKPTTGQKSANMTFQSDKTSGNIVLTVENAAV
                     GYDGDILSQPISIDQRKLDAIAIVGPNGIGKTTLLKSIIGALPFIKGEAKLGANVEVG
                     YYDQTQSALTPSNTVLEELWSAFPTTPEVEIRNRLGAFLFSGDDVKKSVAMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     KVLEISETGSTLYLGDYDYYLEKKAELEVEAQPDQLESTSQSAGAMDYQAQKENQKEQ
                     RKLARRIEQIEAEIETIENRLSELNQAMLETNDIGQLTDYQKEIDQLTSQQESLMEEW
                     EELSEQMG"
     gene            5822..6151
                     /locus_tag="AC1PNG_000480"
     CDS             5822..6151
                     /locus_tag="AC1PNG_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701014.1"
                     /translation="MIQQNTETLEDNLRFNKLSKLIQTKLSQYFETYPSAVQCQLVCF
                     DRDETRRKVLSYDIYSDFDKLFWVESIAKPISSYSYGHQIDKKDIDKLDREFILDTFL
                     GIDEERG"
     gene            6158..6985
                     /locus_tag="AC1PNG_000481"
     CDS             6158..6985
                     /locus_tag="AC1PNG_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RNA methyltransferase"
                     /protein_id="HIP6701015.1"
                     /translation="MTKHQRFLNSDQFFACPHCGQALGLELNSLRCPNRHTFDIAKQG
                     YVNLAPQVKQSANYHKSSFENRQAFLEAGYYDHLYEALERKIAELGLRSVLDIGCGEG
                     FYSRKLAEKMDLDILAFDISKDSILLAAKSDRTKSVKWFVSDLTKLPIQDKTIDGILD
                     IFSPANYQEFARVLKAGGAILKLVPGPNHLKELRHLAKDQLRKESYDNQDIVDHFKSY
                     LGQVEQVLVSRTLPITAAHAQVLADMTPLFFQVDQSKLDLSQLTEITIEGVLLIGTI"
     gene            complement(7025..7810)
                     /gene="gatD"
                     /locus_tag="AC1PNG_000482"
     CDS             complement(7025..7810)
                     /gene="gatD"
                     /locus_tag="AC1PNG_000482"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit GatD"
                     /protein_id="HIP6701016.1"
                     /translation="MVYTSLKSPEKDYTYDLHIAHLYGDLMNTYGDNGNILMLKYVAE
                     KLGARVQVDIVSLTDEFDKNFYDIAFFGGGQDYEQSILAKDLPTKKDSLADFIENDGV
                     MLAICGGFQLLGQYYIEAGGRKIDGLGIMGHYTLNQTNNRYIGDIKIHNDEFNETYYG
                     FENHQGRTFLADNQKPLGKVVYGNGNNKEDGGEGLHYKNTFGSYFHGPILSRNANLAY
                     RLVTTALRKKYGQDIQLASYADILSKEVAEEYGDVKSKAEFEK"
     gene            complement(7810..9153)
                     /gene="murT"
                     /locus_tag="AC1PNG_000483"
     CDS             complement(7810..9153)
                     /gene="murT"
                     /locus_tag="AC1PNG_000483"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HIP6701017.1"
                     /translation="MKVNTALGILAGKSSQFILKKLGRGTTLPGKIALKFDKHILDSL
                     AKDYEVVVITGTNGKTLTTALTVGILQEAFGEITTNTSGANMITGITATFLSAKKNKK
                     GKKIAVLEIDEASLARVTDFIKPSLIVFTNIFRDQMDRYGEIYTTYQMILDGAAKAPQ
                     ATILANGDSPLFNSTNVINPVKYYGFATEEHEPRLAHYNTEGVLCPHCHQIIQYKLNT
                     YANLGSYICNNCGFCRPELDYKLTDLKEITNTSSTFAIDGQDYKINIGGLYNIYNALA
                     AVSVAAFFGVTPEKIKAGFDKSKAVFGRQETFKLGDKDCTLVLIKNPVGATQAIEMMK
                     LAPYDFSLSVLLNANYADGIDTSWIWDADFEQITQMDIPQVFAGGVRSSEIARRLRVT
                     GYPENQITETPKLEDIMALIEQSSSQHAYILATYTAMLEFRDLLAQRQAVGKEMK"
     gene            9335..10189
                     /gene="cdaA"
                     /locus_tag="AC1PNG_000484"
     CDS             9335..10189
                     /gene="cdaA"
                     /locus_tag="AC1PNG_000484"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIP6701018.1"
                     /translation="MLNLNQLLDTGYWSSLIASPWTALLHLIDISIVVYLIYNFSKAI
                     AGTKIMTLIRGVFLFIIVQIIASLFGLQTIAWLINQVITYGVIAAVVIFAPELRAMLE
                     KLGRTTQIFGTNTVSAEEKLIVAFLKSVAYMSPRKIGALVAVEQAQTLQEYRATGIPL
                     NADISQELLINIFIPNTPLHDGAVIVKEDKVAVACAYLPLSESAGISKEFGTRHRAAI
                     GLSEVSDAFVFIVSEETGSISIARNGIFKHDLTLDEFEAELRATFISENVEKKSIWKR
                     LGGGKHER"
     gene            10179..10883
                     /locus_tag="AC1PNG_000485"
     CDS             10179..10883
                     /locus_tag="AC1PNG_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIP6701019.1"
                     /translation="MNDKFKAVGHLALSIFLALLLFFYATTTNYKNSESAAQNTESET
                     YVHTLSNVPIEIEYDTNKYFISGLSSTVAVELRGSNRVLLQRESDESTRTFQVTADLR
                     ELSDGTQTVKLQLTNLPAGVSATLAPDAITVKIGKKVSKAFPVAGRVYSNQLAEGYSL
                     SKVSVNVDTVKVTTDEETMAKIDRVEAVAMDVSNLSENYSGTAKLQAVDSDGNVLPVV
                     LSQTEANMQIILTKTK"
     gene            10896..12245
                     /gene="glmM"
                     /locus_tag="AC1PNG_000486"
     CDS             10896..12245
                     /gene="glmM"
                     /locus_tag="AC1PNG_000486"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIP6701020.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETDVPRV
                     FVARDTRISGQMLEAALIAGLLSVGIHVYKLGVLATPGVAHLVKTEKASAGVMISASH
                     NPAQDNGIKFFAGDGFKLDDALEAEIEALLDAEEDTLPRPSAQGLGDVVEYPEGLRKY
                     QQFLVSTGTDLDGMKVALDTANGAAATSARQIFVDLGADLTVMAEKPDGLNINEGVGS
                     THPEKLQELVKETGSQIGLAFDGDSDRLIAVDENGDLVDGDRIMYIVGKYLADRGLLA
                     KNTIVTTVMSNLGFHKALDREGIEKAVTAVGDRYVVEEMRKEGYNVGGEQSGHVILMD
                     YNTTGDGQLTAVQLTKIMKETGKKLSELAAEVTIYPQKLVNIRVENSMKDKAMEVPAI
                     AAIIEKMEAEMAGNGRILVRPSGTEPLLRVMAEAPTDAEVDYYVDTIADVVRAEIGI"
     gene            12391..13935
                     /locus_tag="AC1PNG_000487"
     CDS             12391..13935
                     /locus_tag="AC1PNG_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIP6701021.1"
                     /translation="MSILEVKNLSHGFGDRAIFENVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGQLQPDEGKVEWSRYVTAGYLDQHAKLEKGQSVRDVLRTAFDELFKTEARIN
                     DIYMSMAEEGADMDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDYGMDKDVT
                     ELSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQLLTRYTGDYYQFQEVHAMKRAQLEAAYERQQKEIADLQDFVN
                     RNKARVATRNMAMSRQKKLDKMEIIELQAEKPKPSFDFKMARTPSRFIFQTTDLEIGY
                     DRVLTRKPLNLTFERNQKIAIVGANGIGKSTLLKSLLGIIPPLGGSVERGEYLELGYF
                     EQEVAGGNRQTPLEAVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQAKVR
                     FCLLMNRENNVLVLDEPTNHLDVDAKDELKRALQAYKGSILMVCHEPDFYEGWVDDVW
                     DFNELT"
     gene            14148..14810
                     /locus_tag="AC1PNG_000488"
                     /pseudo
     CDS             14148..14810
                     /locus_tag="AC1PNG_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775244.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            15003..16178
                     /gene="sstT"
                     /locus_tag="AC1PNG_000489"
     CDS             15003..16178
                     /gene="sstT"
                     /locus_tag="AC1PNG_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HIP6701022.1"
                     /translation="MNLIRKIGIGVVLGVLLGLIAPKITVIALFGSLFVGALKAIAPL
                     LVLTLVAHALSQAPAGQKSNMRTVICLYLFGTFAAAFIAVGASYLFPIKLVLSTTTTT
                     DIIPPQGIAEVFQDLLLKVVDNPINALATANYIGVLTWAAVFGLAFRHASKTTKDLLQ
                     SAAEVTSKVVGWIIGLAPFGIMGLVFDTIANNGLTALKDYGLLLLLLVGSMIFVALVV
                     NPLIAFLVMKKNPYPLVFECIRVSGVTAFFTRSSAANIPVNMQLCKRLGVDPDTYSVS
                     IPLGATINMAGAAITINILTMAAVHTLGISVDFSSALLLSVVASLSAAGASGVAGGSL
                     LLIPVACSLFGIPNELAMQVVGVGFVVGVIQDSCETALNSSTDVLFTVVAERSAWKK"
     gene            16440..17840
                     /gene="pepV"
                     /locus_tag="AC1PNG_000490"
     CDS             16440..17840
                     /gene="pepV"
                     /locus_tag="AC1PNG_000490"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HIP6701023.1"
                     /translation="MTVDFRAEFDKRKDEFLADLFDLLRINSERDDSQADAQHPFGPG
                     PVRALDKFLEIAQRDGYPTKNVDNYAGHFEFGEGDEVLGIFGHLDVVPAGSGWDTDPY
                     EPQIIDGRLFARGSSDDKGPTMACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADMD
                     YYFENVGLPLPDFGFSPDAEFPIINGEKGNITAYLHFAGENSGAAKLHSFTGGLRENM
                     VPESATAIISGDLADLDSKLADFTAAYGLKADAETLENGQIQVTVIGKSAHGSTPEEG
                     VNGATYLAKFLSQFAFDGAAKAYLDLAGQVLLEDHDAQKLGVAIYDEQMGALSMNAGV
                     FKFDETSSDNTIALNFRYPKNTNPETIKAGLEKLGVEAVSLSEHGHTPHYCPIDDPMV
                     ATLLSVYEKHTGLKGHEQVIGGGTFGRLLKRGVAYGAMFPGDVNTMHQANEFIEVEQL
                     YRAAAIYAEAIYELIK"
     gene            17881..18339
                     /locus_tag="AC1PNG_000491"
     CDS             17881..18339
                     /locus_tag="AC1PNG_000491"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6701024.1"
                     /translation="MKLKELTVADLEMVKQLFLSVFSQEPWNDDWSDEEQLDCYIGDL
                     LGHPRALCFGLFDPEKVIGLSLGYIRYWCEGTEYRIEEFCISRDYQGQGHGASLLADI
                     EKQLADRKIVHILLQTERNLSAYSFYKKCGFRALEEDVTMVKKVGHHGTC"
     gene            18326..>18884
                     /locus_tag="AC1PNG_000492"
     CDS             18326..>18884
                     /locus_tag="AC1PNG_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HIP6701025.1"
                     /translation="MELADIRQAIIEDVANRDFTNKGIAPLFQAPSTAKILIIGQAPG
                     LKTQEKGRLFDDASGNNLRQWLGVDRQTFYESGHFAILPMDFYYPGKGKSGDLPPRKD
                     FAPKWHPLIMKQLPQIELTILVGNYAQTYYLEKSQRNLTERVKHADDYLPKYFPLPHP
                     SPRNNIWQAKHPWFVEQTLPILKNNI"
BASE COUNT         5345 a         3750 c         4512 g         5277 t
ORIGIN      
        1 gagttttttg taaattttct tgaaaaaatg tataatagaa gcgatagcta tttaggtatt
       61 tctatttaaa agttgcgttg atggttgaat ataattttga accaaagtgt gtcttttgca
      121 aatagagctc cgacatagca gactgtaatt cgacttggaa ctcccaagga ccgttggtaa
      181 gaaaaataaa atggacacct gtgggatgtc caaaaggagt aaagatgagt acaattaaaa
      241 ttatggctct tgggggtgtg cgggaaaatg gaaaaaacct gtacattgcc gaagtgaatg
      301 aacatatttt tgttttggat gcaggggcaa agtatcctga gaatgaacag ttaggtgtgg
      361 atgtagtcgt cccaaatttc gattatttgg aagaaaataa gcatcgtgta gcaggggtct
      421 ttttatctca cgggcatgca gatgctatcg gagcgcttcc ttatctcttg gagaaagtga
      481 aagtgccagt atttggttcg cacttgacta tcgaattggc taagctactt gtcaagggga
      541 ataatgctac taagaaattt aatgatttcc atgttatcaa tgctgaaact gaaattgatt
      601 tcggagattc agtggtatct ttctttcaga cgacccactc tattccagag agtttaggaa
      661 ttgtcgttaa gacggatgag ggaaatattg tctacacagg tgactttaag tttgaccagg
      721 cggcagataa gttttatcgt acggattttg ggcgattggc tgaaatcggt aacgaaggtg
      781 tgttggccct cttgtcagac tcagctaatg cagatagcaa tgttcaagta gcgagcatgc
      841 atgaggctgg ggaggaaatt ctcaatacga ttgctgactg tgacgggcgt gttattgtgg
      901 cagcagttgc cagcaatatt gtacgtattc aacagatttt tgacgcggca gaagcaactg
      961 gtcgtcgtgt cgtgttgaca ggacatgatg tggaaaacat tgtccgaaca gcgattcaat
     1021 tgaaaaaact tcgtttggtc agcgaacgct tgttggtaaa accaaaggat atgaacaagt
     1081 atgaagacca tgaattgatt attttagaaa ctggtcgtat gggcgagccg ttaaatggat
     1141 tgcgtaaaat gtctatcggt agacatcgct acgttgaaat caaagaaggt gatctagttt
     1201 atattgttac cactccaagt atttcaaaag aagcggttgt tgctcgtgtg gagaacttga
     1261 tttacaaggc tggtggtacc gtaaaatcta tcacaaagaa tcttcgcgtc tctgggcatg
     1321 ggaatgctcg tgatttgcag ctcatgctca acatcatgaa gcctaaatat cttttcccag
     1381 ttcaagggga gtatcgtcaa ttggatgctc acgctaaggc tgcgcttgaa attggtatgt
     1441 atccagaaaa cattatcatc gttaagcgtg gggatgtcat ggcttttgaa gagggggatt
     1501 ttgtccatag cggagcggtt cctgctggtg atgtcatgat tgacggaaat gccatgggcg
     1561 atgttggaaa tatcgtcctt cgtgaccgta aaatcctatc agaagacggt atctttatcg
     1621 tggctatcac tgtcaaccgc cgtgagaaaa aaatcatttc taaagccaag gtcaatactc
     1681 gtggttttgt ctacgtgaaa aagagcaagg acatcttgcg tgaggcatca gaattggtga
     1741 atacaacggt ggaaaattac tttacaaagg atagctttga ctgggcagag cttaagagtg
     1801 ctgttcgcga tgatttagca aaattcctct ttgaccaaac taaacgtcgc ccagctattt
     1861 tgccggtaat tatggaagtc aagtaagctg atttatatac ataagaagag ccgagggaaa
     1921 ctcaggctct ttaccataag gagaagattt atggcaataa tgaaaattga ataccattca
     1981 gaagctctag atatgtctag acaggttacg gttttatatc cagatcgaaa tcgtgtggaa
     2041 aatcccgatg acaacgatat tcctgtccta tatcttttgc acggtatggg aggaaatcag
     2101 gattcctggc tgaatcgttc tacagtggaa cggttggttc gttataccaa tcttatcgtg
     2161 gttatggtca atacagataa aggttggtat accaatacca cttacggtat gaattacttc
     2221 gatgccattg cagtagagtt gcctcaaatt ctcaaacgat ttttcccaaa tatgtctgac
     2281 aagcgggaga agaattttat cgctggtttg tctatgggtg gttatgggac cttcaagatt
     2341 gccatgatga ccaatcgttt ttcacacgca gcttcgcttt caggcgcatt gtattttgat
     2401 tttgataatc cagcggcagc agaattggga agtctttcgt attggcaagg agtatttggt
     2461 gacatttctg ataaacagaa tccgaataat ttgttagaaa ttgctaaaca atccgataaa
     2521 aagactaagt tttatgcctg gtgtggtgag gaagattttc tctttgaagg gcatcaaaag
     2581 gcagttgggg agttgaaaga gctgggattt gatatagagg caagtttcgg acctggtaaa
     2641 catgagtggt actactggaa tcagcagatt gagaaggtat tggcttggtt gccaattgat
     2701 ttcaaattgg aagaaagact gtcctagttt ttaagaaaag tttcagaaat tatggctata
     2761 ctaaaaccta tttctagaaa gaggtatagt ctatgaaact tattggaaat attctacggt
     2821 ggatttggcg gacattttgg ggtctgattt ggttatcaat gatgttattg gcttgtggtc
     2881 taggtctatt attctatttt caacaggaag cagctcctca gatgttacaa actctagctc
     2941 aagaagtgca attgatggtc aaaggacagt ctgaagtgac gattgaggaa ggtgttaata
     3001 ccattaagca cctgacgact gatacggtca atacagctga tcatggtcga tgggaaagca
     3061 atactgcgac tgtttacatt gaaacccaga atccaacatt tgtagctgcc tatgaaacag
     3121 ccattgcaaa ttggaacacg acaggggcat ttacttttgt gatgacaagt gatccaaatc
     3181 aggctgatat tattgctact gagatgaatg atggaaatac ccaggcagca ggtgaggcca
     3241 attcaacgac taatttattg acgaattatt atagctcggt aacggtgcgt ctgaatagtt
     3301 tttatttatt aaacgaccaa tatggttatg atatggatag aattatccat acagctgagc
     3361 atgaattggg ccatgctatt ggcctagatc atgaagatag tcagacctct gttatggaat
     3421 cggcaggttc taatcatggt atccagcagg cagatattga tgcggtcctt gccttgtatt
     3481 cagaatagaa gggaaaatat gcttcgaaca attacaaaca caataaaacg ttatcctgag
     3541 caagctctgc tctttctcta taatgctggg atttttgcat ggatgcagtc taccagtcat
     3601 tcgattatgg aacaaatcgg tatagatagc aactggtttg ataaaatccc cgaacctatc
     3661 aaggcctgga caggtgccag tctagagagt atgcaaacct tactcaattc ttctgcttgg
     3721 ggatggctga ttgtatctat gatattgatg ttggtcatcc gctttgttaa gggcttgatt
     3781 aagtttgtca tcatgctcat cattattggt ggtggtcttt atctgctctg gcagaataag
     3841 gagcttgttc aatccttggt ataaatagag gaatcaaatc agccttgtgc tgattttttt
     3901 ggtataatat tttcatgatt attctaagtg gaaacaagat tgaacgctct tttgcgggcg
     3961 aagttttatt taacaatatc aacattcaag tggacgagcg ggatcggatt gcccttgtcg
     4021 gaaaaaatgg ggcaggcaag tctactctgc ttaaaatcct tgtcggagaa gagactgcga
     4081 caagcggaga gatttcaacc aagcgtgatt tgtcactgtc ttatctggca caggacagcc
     4141 gttttgagtc ggcaaatacc atttacgatg aaatgctcca tgtttttgac gacctgcgga
     4201 cgacggaaaa acgcctgcgg tctatggaag agcagatggg cagcctatct ggtagcgacc
     4261 tagatcaact gatgaaaacc tacgatagtc tgtcagagga atttcgtctg gcaggtggtt
     4321 ttagctatga ggcggatatt cgtgccattt tgaacggctt taagtttgac cagaccatgt
     4381 gggacatgaa aatctctgaa ctctcaggtg gtcagaatac tcgacttgca ctggcaaaaa
     4441 tgcttcttga aagcccagaa ctcttggtcc tggacgagcc gaccaaccac ctggacatcg
     4501 acacgattgc ttggttagaa aactacctag tgcattacaa gggagccttg attatcgtca
     4561 gccatgaccg ctatttcttg gacaaggtgg cgacactgac actggacttg accaagcatt
     4621 ccttggatcg ctatgtgggc aattattctc agtttgtcga gctcaaagag cagaagttgc
     4681 aaacagaatt gcaaaactat gaaaagcaac agaaggaaat tgccaaacta gaagactttg
     4741 tccagaaaaa tattgtccgt gcttcgacca ccaagcgcgc tcaggctaga cgcaagcagt
     4801 tggaaaagat ggagcgactg gacaagccaa caactggtca gaagtctgct aacatgacct
     4861 tccagtcgga caagacttct ggcaatatcg tcttgacagt ggaaaatgct gcggtgggct
     4921 acgatggaga tatcctttct cagcccattt ctattgacca acggaaacta gatgccattg
     4981 ccattgtcgg tccgaatgga atcggaaaaa ctaccctgct caaatcaatc atcggagctt
     5041 tgccatttat caaaggggaa gccaagctag gtgccaatgt ggaagtgggt tactacgacc
     5101 agacccagtc agccctaaca ccttccaata cagtcttgga ggagctctgg tcagcctttc
     5161 cgactacgcc tgaagtggag atccgcaacc gccttggagc cttcctcttc tcaggagatg
     5221 atgtcaaaaa gtccgttgcc atgctgtcgg gtggggaaaa ggcccgtctg ctcctcgcta
     5281 agctatctat ggaaaacaac aacttcctca tcctcgacga gccgaccaac cacctggaca
     5341 tcgacagtaa ggaggtcctg gaaaatgccc ttatcgactt tgacggcacc ctgctctttg
     5401 tcagccacga ccgctatttc atcaaccgcg tcgccaccaa ggtgctcgaa atctcggaaa
     5461 cgggctccac gctctacttg ggcgactatg actattattt ggagaaaaag gcagagctgg
     5521 aagtagaagc ccagcctgac cagctagaaa gtactagcca atcagccgga gccatggact
     5581 accaagccca aaaggaaaac caaaaagagc aacgcaaact agctcgccgc atcgagcaaa
     5641 tcgaagcaga aatcgagaca atcgaaaatc gcctgagcga gctcaaccaa gccatgttag
     5701 aaaccaacga cattggccag ttgaccgatt accagaaaga aatcgaccaa ctgaccagcc
     5761 aacaagaaag tcttatggaa gaatgggaag aattatctga gcagatgggg taagaaataa
     5821 gatgatccaa cagaatacag aaacactcga agataactta cgatttaata aattatccaa
     5881 actgattcag acaaaattgt cccaatattt tgaaacctat ccttcagctg tccagtgcca
     5941 attagtttgt tttgatagag atgaaactcg tagaaaagtc cttagctatg acatttattc
     6001 cgattttgat aagctcttct gggtagagtc aatagcaaag cctatttcta gttattccta
     6061 cggacatcaa attgataaaa aagatataga caaactagat agggagttta tcttggatac
     6121 ttttcttgga atagatgaag aaagaggata gattttcatg accaaacacc aacgtttcct
     6181 taatagcgac caattttttg cctgtccaca ttgtgggcag gctcttggtc ttgagctcaa
     6241 cagcctccgc tgtcccaacc gccacacctt cgacatcgcc aagcagggct acgtcaatct
     6301 ggcaccccag gtcaagcagt ctgctaacta ccacaagtct agctttgaaa accgccaggc
     6361 ctttttagag gcaggctatt acgatcatct ctacgaggct ttggagagga aaatagcaga
     6421 gctgggcttg cggtctgtct tggacatcgg ctgtggagag ggcttttatt cccgtaagtt
     6481 agccgagaaa atggacttgg acattcttgc ttttgatatt tccaaggatt ccattctctt
     6541 ggcagccaaa tctgacagga ccaagtcggt caaatggttt gtcagtgact tgaccaagtt
     6601 acctattcaa gataagacca ttgacggcat cttggacatc ttttccccag ccaattatca
     6661 ggaatttgcc agagtgctga aagctggtgg ggcaattcta aaattagtcc caggccccaa
     6721 tcacctcaag gagctccgcc atttagccaa agaccaactc cgcaaggaat cctatgataa
     6781 ccaggatatt gtggaccatt tcaagtctta cctgggacag gtggagcaag tgctagtcag
     6841 tcgaactctg ccgattacag ccgcccatgc tcaggtcttg gcagacatga cacccctctt
     6901 tttccaagta gaccagtcca agttggattt gagccagttg accgagatta cgattgaggg
     6961 agtgctcttg attggaacta tataaaaaat gaaaccgcct ctggatatca gaagcggttt
     7021 tgctttattt ctcaaactct gccttgcttt tcacatcgcc gtattcttca gcgacttctt
     7081 tgctgaggat gtcggcgtag ctggcaagtt ggatgtcttg tccgtatttt ttgcggaggg
     7141 cagtagtgac caggcggtag gccaggttgg cattgcggga taagattggt ccgtggaagt
     7201 agctaccgaa ggtgtttttg tagtgcagac cttcgccgcc gtcttccttg ttattgccat
     7261 taccgtagac aaccttgccc agcggttttt ggttgtcagc taggaaagta cgcccttggt
     7321 ggttttcaaa accgtagtag gtttcgttga actcatcatt gtgaattttg atgtcaccga
     7381 tgtagcggtt attggtctgg ttgagggtgt agtggcccat gattcctaga ccgtcaatct
     7441 tgcgaccgcc agcttcaata taatattgac cgagcaactg gaagccaccg cagatagcta
     7501 gcatgacgcc atcgttttcg ataaagtctg ctaggctgtc tttttttgtc ggtaaatcct
     7561 tagcaagaat ggattgttca tagtcctgtc caccgccgaa aaaggcgatg tcgtagaaat
     7621 ttttgtcaaa ttcatctgtc agggaaacga tgtccacctg gacacgagcc cccagctttt
     7681 cagccacata cttaagcatg aggatattgc cattgtcacc gtaggtgttc atcaaatcac
     7741 cgtagaggtg ggcgatgtgg agatcgtaag tgtaatcttt ttcaggactt tttaatgacg
     7801 tatagaccat tatttcatct cctttccaac ggcttggcgt tgagccagca ggtcacggaa
     7861 ttcgagcata gctgtataag tagctaaaat ataagcatgc tggctactcg attgctcaat
     7921 caaggccatg atatcttcta gttttggtgt ttcagtaatc tggttctcag gatagccagt
     7981 cacacggagg cgacgggcga tttcagacga gcggacacca cctgcaaaga cttgtggaat
     8041 gtccatttga gtgatttgtt caaagtcagc gtcccaaatc cagctggtat caattccgtc
     8101 agcgtagttg gcattgagaa ggacggacag gctaaaatcg tatggagcca gtttcatcat
     8161 ttcaatagcc tgagtcgcac cgaccgggtt tttaatcaag accagtgtac aatctttatc
     8221 acccagcttg aaggtttctt ggcgaccgaa gactgccttg gacttgtcaa aacctgcctt
     8281 gattttttca ggagttacac cgaaaaatgc tgctacgctg acagctgcta gggcattgta
     8341 gatgttgtag aggcctccga tgttgatttt gtaatcctga ccgtcaatgg caaaggtaga
     8401 agaagtattg gtgatttctt tcaagtctgt caacttatag tccagctctg gtcggcaaaa
     8461 gccacagttg ttacagatat agctgcccaa gttagcgtag gtattgagct tgtactggat
     8521 aatctggtgg caatgaggac agagaacgcc ttcggtattg taatgggcca gacgaggttc
     8581 atgttcttcc gtcgcaaaac cgtagtattt tactggattg ataacgtttg tcgagttaaa
     8641 aagtggacta tcaccattgg ccaaaatagt tgcctgcgga gcttttgcag ccccgtccaa
     8701 aatcatctgg taggtggtat aaatctcacc gtagcggtcc atctggtcac ggaagatatt
     8761 ggtgaaaaca atcaggctcg gcttgataaa gtcggttaca cgggcaagac ttgcttcgtc
     8821 aatctccaag acggcaatct tcttgccctt tttatttttc ttggcagaca aaaaggttgc
     8881 tgtaattcct gtgatcatat tggcaccgct ggtattagtc gtgatttctc caaaggcttc
     8941 ttggagaatc ccaacggtca aggcagttgt taaagtctta ccattggttc cagtaataac
     9001 aacgacttca taatctttag ccaaactatc taaaatatgt ttatcgaatt tgagggcaat
     9061 tttcccaggc agagtagtac cacgtcctag ttttttcaaa atgaactggg aactttttcc
     9121 tgctagaata cccaaagctg tatttacttt catgggacta ttttatcata aagactgggg
     9181 attttctaga atggagtgtg ttggaacgtg atatattcct gcagtaaatc tgtaatattt
     9241 ttaacaggaa ggtcactatt ttctaaagaa aaggggaaaa aatatggtat aatgagtaca
     9301 ttactatgta tgagttagaa agtatgagaa tgatatgtta aacctaaacc agttactgga
     9361 tacagggtat tggtcgagtt tgatagccag tccttggacc gcgctgctgc acctaattga
     9421 tattagtata gttgtttatt tgatttataa ttttagtaag gctattgcag gtactaaaat
     9481 tatgacattg attcgagggg tttttctctt cattattgtc cagattatag cgagtttatt
     9541 cggtctgcag acgattgctt ggctgattaa tcaagtaatc acgtatgggg ttattgcggc
     9601 agtagttatt tttgcaccag agttgcgtgc tatgcttgaa aaactcggta ggacaactca
     9661 gatttttggg acaaatacag tgagtgcaga agaaaagtta attgttgctt ttctcaaatc
     9721 ggttgcttat atgtctccgc gtaaaatcgg agcgcttgtg gcagtagaac aagcccaaac
     9781 attgcaagaa tatagagcaa cggggattcc acttaatgcg gatatatcac aggaattgtt
     9841 aataaatatt ttcatcccga atacaccgtt acatgatggg gctgtaattg tcaaggaaga
     9901 caaggtagct gttgcttgtg cttatttgcc cctgtcagag agcgcaggaa tttcaaagga
     9961 atttggaaca cgacatcgtg cagccattgg cttatcggag gtatctgatg cgtttgtatt
    10021 tattgtctct gaagaaactg gcagtatctc tattgcacga aacgggatat tcaaacatga
    10081 tttgactttg gatgagtttg aagcagagtt gcgagcgact tttatttctg aaaatgtaga
    10141 gaaaaagtcc atctggaagc gattaggagg tggcaaacat gaacgataag tttaaagctg
    10201 taggtcactt agccttatct atttttctag cattgttgct ctttttctat gcaacaacca
    10261 ccaactataa aaattcagaa tcagctgcac agaacacgga gtcagaaacc tatgttcaca
    10321 cactgagcaa tgtaccgatt gaaattgaat atgatacaaa caagtatttt atttctggac
    10381 tttcttcgac ggtggcagtt gaattaaggg gctctaatcg tgttctccta cagagagaat
    10441 cagatgagtc aactcggacg tttcaagtga ctgcggattt gagagagttg agtgatggga
    10501 cacagactgt caagctacaa ttgaccaatt tgccggctgg agtgagtgcg acactagcac
    10561 ctgatgcgat taccgtcaag attggtaaaa aagttagcaa ggcattccca gtagcaggtc
    10621 gagtatatag taatcaattg gcagaaggtt acagtctgtc taaggtttca gtcaacgtag
    10681 ataccgttaa agtgacaacg gatgaagaaa caatggctaa gattgatcgg gtcgaagctg
    10741 ttgcgatgga tgtgagcaat ctgtctgaga attatagtgg tacagctaaa ttacaggctg
    10801 tagatagcga cggcaatgtc ttgccagttg tactgtctca gacggaagca aatatgcaaa
    10861 ttatacttac aaaaacaaaa taagaggtta tgaaaatggg taaatatttt gggacagatg
    10921 gtgtccgtgg agaagcaaac gtagaattaa cgccagaatt agcattcaaa ttgggtcgtt
    10981 ttggtggtta tgtccttagc cagcatgaga cggatgttcc tcgtgtcttt gtggcgcgtg
    11041 acacacgtat ctcaggtcaa atgttagagg ctgccttgat tgcaggactt ctatcagtag
    11101 ggattcatgt gtataaattg ggagttttgg cgacaccggg tgttgcccac ctagtcaaaa
    11161 ctgaaaaagc aagtgcaggt gtcatgattt ctgcaagtca taacccagca caagataacg
    11221 gtatcaaatt ctttgctggt gatggtttca aattggatga tgcattggaa gcggaaattg
    11281 aggctcttct tgacgctgag gaagacacat tgccacgccc atctgctcaa ggtttaggag
    11341 atgtggtaga atatccagaa ggtctacgta agtaccagca attcttggtg tcaactggta
    11401 cagatttgga cggtatgaaa gttgccctcg atacagccaa tggtgccgca gcaacatctg
    11461 cacgtcaaat ttttgtagat ttgggagcag atttgacagt aatggctgaa aagccagacg
    11521 gtttaaatat caatgagggt gttggttcaa ctcatcctga aaaattgcaa gaactggtca
    11581 aggaaacagg aagtcaaatt ggacttgcct ttgatggtga cagtgaccgc ttgattgccg
    11641 tcgatgaaaa tggtgattta gtagacggtg accgtatcat gtacatcgtt ggtaaatacc
    11701 ttgctgatcg aggtctattg gccaaaaata caattgtgac cacagttatg tctaaccttg
    11761 gtttccataa ggctttggat cgtgaaggta ttgaaaaagc tgtgaccgca gttggtgacc
    11821 gctatgtggt tgaagaaatg cgcaaggaag gctacaatgt cggtggtgag cagtcaggac
    11881 acgtgattct gatggattat aataccacag gcgacggcca gttgaccgct gtgcaattga
    11941 ccaaaatcat gaaagaaact ggtaagaagt tgtcagaatt ggcagcagaa gtgaccattt
    12001 acccacaaaa attggtcaat atccgtgtgg aaaacagcat gaaagacaag gcaatggaag
    12061 tgcctgctat tgcagctatc attgaaaaaa tggaagcaga aatggctggc aacggtcgta
    12121 tcttggtacg cccaagtggt actgagcccc tcttgcgtgt catggcagaa gcgccaacgg
    12181 atgctgaagt cgattactac gtggacacca ttgccgatgt ggtccgtgcg gaaattggaa
    12241 tttaagcaga aatcaatcat ttgttaagat gatttcattg tcttgaacta ccagattgat
    12301 taggtggcta gtagttccac tcaagactgc gagaataaaa gtaaaaactg gagtttggtt
    12361 actccagttc tttgatgttg tgaggaaatt atgagtattt tagaagtaaa gaatttaagt
    12421 catggttttg gtgaccgcgc aatttttgag aatgtctcct tccgtctctt gaaaggggag
    12481 catatcggtc ttgtcggtgc caatggagaa gggaaatcga ccttcatgtc catcgtgaca
    12541 ggtcaattgc agccagatga aggtaaggtt gaatggtcac gttatgtaac ggcaggttat
    12601 ttagaccagc atgctaaatt agaaaagggt caatcagtcc gtgatgtctt gcggacagca
    12661 ttcgatgaac tcttcaagac agaagcccgt atcaatgaca tttacatgtc tatggcagaa
    12721 gagggtgccg atatggatgc cctcatggaa gaggttggtg aactgcagga tcgtctggaa
    12781 agccgtgatt tctatacgct tgatgccaag attgacgaag tagcgcgtgc gcttggtgtc
    12841 atggactatg gcatggacaa ggatgtgacg gagttatcag ggggacaacg aactaaagtt
    12901 cttcttgcaa aattgctcct ggaaaagcct gacatcttgc ttctggatga gccgaccaac
    12961 tacttggatg ctgagcatat tgactggctc aaacgctact tgcagaacta tgaaaatgcc
    13021 tttgtcttga tttcgcatga cattcctttc ttgaatgatg tgataaacat tgtctaccat
    13081 gtggaaaatc agctcttgac tcgctataca ggtgattact accaattcca agaagtccat
    13141 gctatgaagc gggctcagtt ggaggcagct tatgagcgcc agcagaagga aattgcagac
    13201 ttgcaggact tcgttaaccg taataaggcc cgtgttgcga ctcgaaacat ggccatgtct
    13261 cgtcagaaga aattggacaa gatggagatt attgagcttc aagcagagaa accaaagcca
    13321 tcatttgatt tcaaaatggc tcgaactcca agtcgcttta tcttccaaac gactgatttg
    13381 gaaattggtt atgaccgtgt attgacacgt aaaccgctca atctgacctt tgaacgcaac
    13441 cagaagattg ccattgtcgg tgccaacggt atcggtaaat ccactcttct taaaagtttg
    13501 ctggggatta ttccaccgct gggcggttct gtagagcgtg gggagtattt ggaactaggc
    13561 tatttcgagc aagaagtagc tggtggcaat cgtcagactc cgcttgaagc cgtttgggat
    13621 gcctttccag cccttaacca ggcggaagtt cgggcagcct tggctcgttg cggtctgact
    13681 tccaagcata tcgaaagcca aattcaagtt ctttcaggtg gtgagcaagc caaagttcgc
    13741 ttctgtttgc ttatgaaccg tgaaaacaat gtcttggttc tcgacgagcc gaccaaccac
    13801 ttggatgtgg atgccaagga tgaactcaaa cgtgcccttc aagcctacaa gggctctatc
    13861 ctcatggtct gccacgagcc tgatttctat gaaggctggg tggacgatgt atgggatttt
    13921 aatgaattga cttagattga aagctatcga acgatagctt tttctttgtg cttatactat
    13981 aatagaatga gcatagtaaa ggaggtaaaa aatgaaaaga acagaacgag gccagatttg
    14041 ctaggcctct ctagaggaag taaaacggtc tggggataga ccgttttagc tcaacaacta
    14101 gaaataaaga cttgttgacg aactcttttt gaccagtcga gttctttccc actcgcgttt
    14161 ctttgacggt tggaaagaca gtaccgcttg actggtttga aaaagcccaa ggtaagaaat
    14221 tgctgggtct agcatgcggt ggtggtcaac agggaccaat ttttgcggct catggctatg
    14281 agacaactat tttggacttc tccaaggaac agttggacaa ggataggtta gttgctgagc
    14341 gggaaaatct ggatttgcaa acggtccagg cagatatgac acaagccttt ctttttgaag
    14401 acgagagttt tgatatcatc ttttgtcctg tttcaaatgt ttatgtcgaa gatttgacca
    14461 atatgtggca ggagtcttat cgggtcttga aaaaaggagg cttgctcatg gttggctaca
    14521 tgaacccttg gatttatgta tttgacagtg acgatgtttg ggattatcca gacaagacct
    14581 tggttccaaa gtatagccta cctttcaacg cacgacaatt agaagaagca ggacaaatca
    14641 cgattgatcc agaatatggc tatgaattta gccacacctt agaagaacag attgctgggc
    14701 aattacgagc tggttttgcc atgattgatt tttatgaatc caaagacagt cgcaatcgct
    14761 tgactcaata tgcctcagat tacctagcga atttatcgat aaagtggtag aaacaagcca
    14821 ccgaaagatg gtcttttcaa ctcagtaggt aaagttgaaa tgattgtgca agatttttca
    14881 ggcttccgct gtataatatc tcatttttta tgctattgac ctgactggat ttttctgcta
    14941 tgattgaaaa atgtttgttt ggaggtaaat gagtgaataa aataatatct gtatggaaaa
    15001 aaatgaattt aatccgaaaa atcggaattg gtgttgtttt gggagtgtta ctaggcttaa
    15061 ttgctccaaa aataacggtc attgctctct ttggtagtct atttgtagga gcattgaaag
    15121 ctatagcccc tcttttggtt ctgacactag ttgcacatgc tctatcacaa gctcctgctg
    15181 gtcaaaaatc gaatatgagg acagttatct gcttgtatct atttggcact tttgcggcgg
    15241 ccttcattgc tgtaggagca agctatttat tccctattaa actggttctt tctactacaa
    15301 cgactacgga cattattcct ccacaaggga ttgctgaagt attccaagac ctcttgttga
    15361 aggtcgttga caatccaatc aatgctcttg caacggctaa ttatattggt gtgttgacat
    15421 gggcggcagt gtttggcttg gcctttcgtc atgcaagcaa gacaaccaaa gatttattgc
    15481 aatctgcggc agaagtgact tctaaagttg taggctggat tattggtctg gcaccatttg
    15541 gtatcatggg cttggttttc gataccattg caaataatgg tctgacagct ttaaaagact
    15601 atggtttact gctcttactc ttggttggaa gcatgatttt tgtcgcgctt gttgtcaatc
    15661 ccttgattgc ttttcttgta atgaagaaaa atccttaccc gctggtattt gaatgtattc
    15721 gggttagtgg ggttacggca ttttttacaa gaagttcagc agctaatatc cctgtcaata
    15781 tgcagctctg caaacgttta ggagtggatc ccgatacata ctcagtatcc attccgctcg
    15841 gtgccacaat taatatggct ggcgcggcta ttacgattaa tattttaaca atggcagctg
    15901 ttcatacact tggcatcagt gttgatttca gctcagctct tcttttatcc gttgttgcat
    15961 cactatcagc agcaggagca tctggggtag caggtggctc gcttctcctc atcccagtag
    16021 catgtagtct ttttggtatt ccaaatgagt tagccatgca agtagttgga gtcggatttg
    16081 tggtcggagt gattcaggac tcttgtgaaa cagccctcaa ctcatcaacc gatgttttgt
    16141 ttacagttgt agcagaacga tctgcttgga aaaaatagaa agaagttggg agaccaactt
    16201 tttttcttcg ctttacttct gaaatctctt tcattttgtg ataaaataga cttactttga
    16261 tatagtcttt gggtttactt ttagaactag gcaacgagcc gcaggcataa ctggagttag
    16321 gcaaggtgag ttcaaacact ccagtggagt gtttgaagtt ggaaataagg aaacgatgtt
    16381 tcctcgctcg tcgaagtaat aaaagataaa ccaaatgacg acactgaaaa ggagaacata
    16441 tgacagtaga ttttagagca gagtttgaca aacgcaaaga tgagtttcta gcggacctct
    16501 ttgacctctt acgcatcaat tctgagcgtg acgacagcca ggcagatgcc cagcacccat
    16561 ttgggccagg accagtgcgt gccttggaca agtttctaga aattgctcag cgtgatggct
    16621 atccgaccaa gaacgttgac aactacgcag gtcactttga gtttggtgaa ggcgacgaag
    16681 tccttggtat tttcggtcac ttggatgtgg tgccagcagg aagcggatgg gataccgacc
    16741 cctacgagcc gcaaatcatc gacggtaggc tctttgcccg cggctcctct gacgacaagg
    16801 ggccaaccat ggcttgttac tacggcttga aaatcatcaa ggagttgggc cttccgacct
    16861 ctaaaaaagt ccgtttcatc gtcggtaccg atgaagagtc aggctgggcg gacatggatt
    16921 actactttga gaacgttggt ctgccattac cagatttcgg attctctcca gacgctgagt
    16981 tcccgattat caacggcgaa aagggaaata tcacagctta tcttcatttt gcgggggaaa
    17041 atagcggagc tgctaaactg cattccttca caggcggcct gcgtgagaac atggtgcctg
    17101 aatctgcgac agctattatc tctggcgacc tagcagacct ggacagcaag ttggcagatt
    17161 tcacagcagc ctatggccta aaagctgacg cggaaaccct tgaaaacggt caaattcaag
    17221 tcaccgtcat cggaaaatca gcccacggtt caaccccaga agaaggtgtc aacggagcaa
    17281 cctatttggc aaaattcctt agccaatttg cctttgatgg agcagccaaa gcctatcttg
    17341 acttagcagg acaagtcctt ctggaagacc acgatgccca aaaactcggc gtagccatct
    17401 acgatgagca aatgggagct ctttctatga atgcaggtgt ctttaagttt gatgaaacct
    17461 catctgacaa cacaattgcc ctcaacttcc gttatccaaa aaataccaac cctgaaacca
    17521 tcaaggctgg tttggaaaaa ctgggcgtgg aagctgttag cctgtctgag catggccata
    17581 ccccacatta ctgcccaatc gatgacccaa tggtcgcaac cctcttgtct gtttatgaaa
    17641 aacacacagg cttgaaaggt cacgaacaag taatcggtgg cggaacattt ggacgcctgc
    17701 tcaaacgtgg cgttgcctac ggagccatgt tcccaggcga tgtcaacacc atgcaccaag
    17761 ccaacgaatt tatcgaagtc gagcaactct accgcgccgc tgccatttac gcagaagcta
    17821 tctacgaact cattaagtaa tacatgaaga cgggtacgcc cgtctttttt aggaggaaga
    17881 atgaaactga aagaactcac cgttgcagat ttggaaatgg tcaaacagct cttcttgtct
    17941 gtctttagcc aagaaccttg gaatgatgat tggtcagatg aggaacaatt agattgctac
    18001 ataggcgatt tactgggaca cccaagagca ctctgttttg gcctgtttga cccggaaaaa
    18061 gtgatagggc tgtcacttgg ttacattcgc tattggtgtg agggaacaga gtatcgaatt
    18121 gaagagttct gcatttcccg tgattaccaa gggcaaggac atggtgctag tttattagct
    18181 gatattgaga agcagttagc tgatagaaaa attgttcata ttcttctgca aaccgaacgc
    18241 aacctttcag cctattcttt ctacaaaaaa tgcggtttcc gagccttaga agaagatgtg
    18301 accatggtca agaaagtagg acatcatgga acttgctgat attcgtcaag ccatcattga
    18361 ggatgttgct aaccgagact ttacgaacaa gggaatagct ccccttttcc aagctccttc
    18421 gaccgcaaaa attctcatta ttggacaggc acctggcttg aaaacccagg aaaaagggcg
    18481 tttatttgac gacgccagtg gtaacaacct tcgtcaatgg ttgggcgtgg atcgacagac
    18541 tttttatgaa tcgggtcatt ttgctattct tcctatggat ttctattatc caggaaaagg
    18601 gaagtctggg gatttgccgc ctcgcaagga ttttgcgccc aaatggcacc cactcattat
    18661 gaagcaactc ccccagattg agctgaccat attggtaggc aattatgctc aaacttatta
    18721 tttggaaaaa tcccaaagga acctgacgga gagagtgaaa catgcagatg attaccttcc
    18781 caagtatttt cctttgcccc atccatcacc tagaaacaat atctggcaag ctaaacatcc
    18841 ttggtttgtt gaacagactc taccgatact gaaaaacaac ataa
//
LOCUS       DBNFEG010000020        18732 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.020, whole genome shotgun sequence.
ACCESSION   DBNFEG010000020 DBNFEG010000000
VERSION     DBNFEG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18732
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.020"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            85..450
                     /locus_tag="AC1PNG_000493"
     CDS             85..450
                     /locus_tag="AC1PNG_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIP6701026.1"
                     /translation="MTVKINTKDGQIELTDDVIATVVGGATTEIFGVVGMASKSAIKD
                     NFQALLRKENYSKGVVIKTLEDGRIAVDVYTVMSYGVKISEVSRNIQERVKFNLENQL
                     GISVDAVNVFIQNIKVVGE"
     gene            453..2117
                     /locus_tag="AC1PNG_000494"
     CDS             453..2117
                     /locus_tag="AC1PNG_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIP6701027.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTITNGAKEVADKSASTVGEVAQILSKGLLMGARGNSGVITSQLFRGFGQSVKGKVE
                     LDGKDLAQAFQHGVEVAYKAVMKPVEGTILTVSRGAATAALKKAEETDDAVEVMRATL
                     DGANRALKKTPEMLPVLKEVGVVDSGGQGLVYIYEGFLSALTGEYIASEDFEATPAVM
                     SEMINAEHHKSVAGHVATEDITFGYCTEIMVALRQGPTYVKDFDYEEFRNYLNDLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQAGLQYGSLVKVKVDNMREQHEAQVEKVESFQKPA
                     EQKEYAIIAVAAGEGLTEIFKSQGVDYVISGGQTMNPSTEDFVNAIELVNARNVILLP
                     NNKNILMAAQTAAEVAEVAVKVVETKTLPQGFTSLLAFDPSRDLESNFEAMTASLAEV
                     KSGSVTTAVRDTTIDGLEIHENDNLGMVDGKIVVSNPDMMETLVATFDKMLDEDSEIV
                     TIYIGEDGDEELAQTLAEQLEEQFEDVEVEIHQGNQPVYPYLFSVE"
     gene            2218..2658
                     /gene="tsaE"
                     /locus_tag="AC1PNG_000495"
     CDS             2218..2658
                     /gene="tsaE"
                     /locus_tag="AC1PNG_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIP6701028.1"
                     /translation="MYSQNENELIAIGERIGKACKPNQVLVLSGDLGAGKTTLTKGLA
                     KGLKIEQMIKSPTYTIVREYEGTMPLYHLDVYRIGDDPDSIDLDDFLYGGGLTVIEWG
                     ELLDVSLFDDYLLIRIEKEGDGRRLTVESHGAQSSDLAKEFQNV"
     gene            2651..3178
                     /locus_tag="AC1PNG_000496"
     CDS             2651..3178
                     /locus_tag="AC1PNG_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIP6701029.1"
                     /translation="MSEKEVYFSEAEPADAAAFIDFMNQVARETDYLVMDETGFRFSV
                     DEMETIFEAGIENPRELCLLAKVGSEVVGAISVKSSKQFRISHIGNIFIAVKKAYWGH
                     GLGTILLEEVLHWAEEMDVLKRLELTVQVRNQAAVHLYQKLGFNIEGTQIRGARTDEG
                     EWLDLYYMGKLIGEV"
     gene            3175..4368
                     /locus_tag="AC1PNG_000497"
     CDS             3175..4368
                     /locus_tag="AC1PNG_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIP6701030.1"
                     /translation="MTIGKKIFLMSLAIIGLTVGAGLIYGASLLNFSTDAISKTFKQL
                     DGEEKITPIDATEPLTILLMGVDMDQATRGGDWEGGRSDSMILVTVNPKTKETNMMSL
                     TRDIMVEIAEANGESSGTVEKLNHSYSYGQAPMAIATIEKMMDINIDRYIEINMDGLV
                     ELVDAVGGIEVNNTLGFPISISEHEPAYTSIVQPGKQLVNGDQALVYARMRYDDPEGD
                     IGRQRRQREVITAIIKKLLQLDGFTQYKKILTAISNNMRTNIEISPATIPNLLGYKDS
                     ISKLNSYQLRGVDQMVDAIYYQLPTSTHLLEMQNVLKKSIGLEENTDLVTNVKVYEGQ
                     MGLPSPINVYDVYTNLLLLEASPWAESLDPEIGSPSATTTVTTEEQVTDFSTEPVLGD
                     ENGSQ"
     gene            complement(4477..4779)
                     /locus_tag="AC1PNG_000498"
     CDS             complement(4477..4779)
                     /locus_tag="AC1PNG_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HIP6701031.1"
                     /translation="MKLRNIILAVSAATASFLAVTHRDKIAKEVQETKQLLSDIQLNK
                     DNIQEQLAIIQSFQEPLQEMASDLQYKTRVYQQSIAGNLEEIQQILKKYQNENNTP"
     gene            complement(4781..5194)
                     /locus_tag="AC1PNG_000499"
     CDS             complement(4781..5194)
                     /locus_tag="AC1PNG_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIP6701032.1"
                     /translation="MSDCIFCKIISGEIPASKVYEDDQVLAFLDITQATKGHTLVVPK
                     KHYRNVLDMDKEAAATLFSVVPTIARQLKEKLGASGLNIVNNNEEAAGQTVFHTHIHL
                     LPRFNNQDGLSIQFKANDPDFPALAQLAQELYLGE"
     gene            5257..5991
                     /locus_tag="AC1PNG_000500"
     CDS             5257..5991
                     /locus_tag="AC1PNG_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6701033.1"
                     /translation="MLEVKNVTGGYVNIPVLKDVSFTVEDGQLVGLIGLNGAGKSTTI
                     KEIIGLLTPYQGQIAIDGLTLLQGAENYRKKIGFIPETPSLYEELTLKEHLEVVAMAY
                     DLSWEQAWGRVEHLLKLFRLDEKLDWFPVNFSKGMKQKVMIICAFMVEPSLLIVDEPF
                     LGLDPVAISDLIALLGEEKSKGTSILMSTHVLDSAEKMCDSFVILHQGQVRATGNLEE
                     LQSAFGMDGASLNEIYLALTQEGVAG"
     gene            5988..7028
                     /locus_tag="AC1PNG_000501"
     CDS             5988..7028
                     /locus_tag="AC1PNG_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6701034.1"
                     /translation="MKELFQQRRLDFLNRCSKYLRYVLNDHFVLVLMVLLGFLSLQYR
                     QLLLSFPENPLPIVFVVIAISLLVLVSGRIATYLEEADQIFLLTKEKDILQGLEVAAR
                     CTVLFWSSIQVVVQLVLLPIYLKLGLPVWMIVAGLLTLLVLKCLFVKRQLVAYQSQGV
                     LDWSKAIQDEQKRKQSILRFFALFTTVKGISTTVKPRSYLNGILRLVKSRQTWFYLYL
                     RAFLRSGDYLGLTIRLLLLAILSLFAIEESWLSIGLVLVFHYLLVFQLLGLYKHYDYQ
                     YITQLYPLEKDSKKIGLQNLLRVILYSLLLVELGFALLFSKENTMVVVLLLVGIFLHE
                     IYLPLKLKKLID"
     gene            7078..7872
                     /gene="ccrZ"
                     /locus_tag="AC1PNG_000502"
     CDS             7078..7872
                     /gene="ccrZ"
                     /locus_tag="AC1PNG_000502"
                     /EC_number="2.7.1.15"
                     /EC_number="2.7.1.229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0051726 - regulation of cell cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle regulator CcrZ"
                     /protein_id="HIP6701035.1"
                     /translation="MQFDTSGLQLQSIAGNSGKAFKGLRSDGTPVFVKYEMPPIVSAL
                     AREQITPPVLSANREVGMGHRVEQEWLNGRTLERQDMTSKQIRQILVRMHYSRILLNQ
                     ALQMNYTYMEPQDLVYRWQEEAPTRLGQNSYLQSICQDLLNNLPRFRQEVATFVHGDL
                     HHSNWVETTSGLVYLTDWETACVTDRMLDVAYILTHYIPRQSWEEWLRAYGYKYNSTV
                     LNKVYWYGQLGYLNQIAKHVESYNVLAANKEIYALRQFRQSFYKEV"
     gene            7869..8507
                     /gene="trmB"
                     /locus_tag="AC1PNG_000503"
     CDS             7869..8507
                     /gene="trmB"
                     /locus_tag="AC1PNG_000503"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIP6701036.1"
                     /translation="MRVRNRKGASELLANNPQYVISNPEECKGKWAEIFGNNNPIHIE
                     VGSGKGRFVTGMAAQNPDINYIGIDIQMTVLSYALDRVLEAGLPNIKLLQVDGSSLTN
                     YFAPAEIDQLYLNFSDPWPKKRHEKRRLTYKSFLDTYKEILPEKGEIHFKTDNRGLFE
                     YSLASFSQYGMVLKQVWLDLHADGLEGNVMTEYEEKFSNKGQVIYRVEAQFQ"
     gene            8765..9235
                     /gene="rimP"
                     /locus_tag="AC1PNG_000504"
     CDS             8765..9235
                     /gene="rimP"
                     /locus_tag="AC1PNG_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIP6701037.1"
                     /translation="MSSIIELVTAAITPAIQTPYELVDVEYGKMGGDYVLSIFVDKEG
                     GISLQDTADLSEKISPILDTIKPDPFPDQYMLEVTSPGLERPLKTADAVEKAVGKYIH
                     VKLYQAIDKIKVFEGTLLSFDGTDLIMEYMDKTRKKEVTIPYQTVAKARLAVKL"
     gene            9308..10450
                     /gene="nusA"
                     /locus_tag="AC1PNG_000505"
     CDS             9308..10450
                     /gene="nusA"
                     /locus_tag="AC1PNG_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIP6701038.1"
                     /translation="MSKEMLEAFRILEEDMGINKADIIDAVTESLRSAYKRRYGQSES
                     AVIEFDEKKGDFHVFTVREVVDEVFDSRLEISLKDALAISSAYEMGDKIKFQEDPAEF
                     GRVAAQSAKQTIMEKMRKQKRAITFNTYKQHENEIMSGTVERFDNRFIYVNLGTIEAQ
                     LSKQDQIPGEVFQSHDRIEVYVYKVEDNGRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGSNIKKITSKFHPARYDAKNDR
                     MIPTEENIDVIEWVADEAEFIYNALAPAEVDQVLFDTEDGKHATVVVPDDKLSLAIGR
                     RGQNVRLAAHLTGFRIDIKSASEYEAFEATQYATDEVVEEVAEEQE"
     gene            10467..10763
                     /gene="rnpM"
                     /locus_tag="AC1PNG_000506"
                     /gene_synonym="ylxR"
     CDS             10467..10763
                     /gene="rnpM"
                     /locus_tag="AC1PNG_000506"
                     /gene_synonym="ylxR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P modulator RnpM"
                     /protein_id="HIP6701039.1"
                     /translation="MAKARKIPLRKSVVSGEIIDKRDLLRIVKNKEGQVFIDPTGKAN
                     GRGAYIKLDNDEASQAKKRRVFDRSFSMEVAEEFYDELIAYVDHKIKRRELGLE"
     gene            10720..11058
                     /locus_tag="AC1PNG_000507"
     CDS             10720..11058
                     /locus_tag="AC1PNG_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HIP6701040.1"
                     /translation="MLIIRSRGENLDSNKRQKILNLLGLAQRAGRLVSGEDLVVEAIQ
                     KGQAKLVFLAEDAAGNLSKKVTDKSHTYQVEVVTVFSTLELSAAVGKARKVLAVTDAG
                     FTKKMRSIME"
     gene            11076..11193
                     /locus_tag="AC1PNG_000508"
                     /pseudo
     CDS             11076..11193
                     /locus_tag="AC1PNG_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
     gene            11697..13898
                     /gene="infB"
                     /locus_tag="AC1PNG_000509"
                     /pseudo
     CDS             11697..13898
                     /gene="infB"
                     /locus_tag="AC1PNG_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
     gene            14054..14407
                     /gene="rbfA"
                     /locus_tag="AC1PNG_000510"
     CDS             14054..14407
                     /gene="rbfA"
                     /locus_tag="AC1PNG_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIP6701041.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMVGD
                     LSMAKVYYTIMSNLASDNQKAQTGLEKATGTIKRELGRKLTLYKIPDLVFEKDQSIEY
                     GNKIDQMLRALDQKD"
     gene            14689..15360
                     /locus_tag="AC1PNG_000511"
     CDS             14689..15360
                     /locus_tag="AC1PNG_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin lysis protein"
                     /protein_id="HIP6701042.1"
                     /translation="MKTFISKLMLVSTVVLLAACQSNQVKGGESSSSSASSQVSDGSS
                     LQQASTIASSSETQASSVTTPELSEVYQSVIDRYQANMGQSAEAINQDEVSSYLSLLT
                     SQGQEYSGIFYSLYDVNHDGTEELLLALDKSGEYVLIDLYTQLADESLRLVDNFRNLG
                     FEIGPNALLRPLQDGTYLFEGDGIFRIYQYNAMIPGLKRISESDTNPETSPLLELKSL
                     HWVKL"
     gene            15529..16596
                     /locus_tag="AC1PNG_000512"
     CDS             15529..16596
                     /locus_tag="AC1PNG_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HIP6701043.1"
                     /translation="MKTNLKKLLYSGVTLMSLGVLAACSSTSSSTTTSSSAATSQTSV
                     ATTSNNSTSSDSSSSTIDWSALPTTEVTLSNDGLKITEGGTYILTGSTTAGVTVETDA
                     NVRIILAGAEISSSDTAAINVINADNVELEIKDGTTNTVKDTSNHTDTNIEGAIHVEA
                     DLTITGNGSLTVEGNFQDGIVSTDDLVVNSGNITVTAVDDGIRGKDSLTINGGEITVT
                     AGDDGIKAETALTIDGGTITVSESAEALEGTNITINGGTLDVYGSDDAINAASTTSSE
                     IFIKVTGGDLKVAVGSGDTDAFDANGDIYISGGTIDVTAQSAFDFDGTAELTGGTVTV
                     NGEQITQITATGPGAGGHGGW"
     gene            complement(16632..17291)
                     /locus_tag="AC1PNG_000513"
     CDS             complement(16632..17291)
                     /locus_tag="AC1PNG_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HIP6701044.1"
                     /translation="MEFDLFLMICNYIGTIAFAVSGAVKGFKKKLDIFGISLLGIITA
                     VGGGIIRDTMANRIPAALTDPTAIYLSLGVSISMYLIVINLKQDKPLDKKMIHFLSQT
                     NLIFDAIGLAIFALIGASTGISLQLNALTSGILAALTGVGGGIARDLLVNETPIVLKE
                     DVYAVLAFFSGVLYHLCVVNWKLPQIPTFITIFTISLIIRLLVIKYKINLPNMESKRK
                     V"
     gene            17524..18492
                     /locus_tag="AC1PNG_000514"
     CDS             17524..18492
                     /locus_tag="AC1PNG_000514"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIP6701045.1"
                     /translation="MVSITKEQHLDMFLKMQQIRDVDMKLNKLVRRGFVQGMTHFSVG
                     EEAAAVGPIAGLTDEDIIFSHHRGHGHVIAKGIDINGMMAELAGKATGTSKGRGGSMH
                     LANVEKGNFGSNGIVGGGYALAVGAALTQQYLGTDNIVIAFSGDSATNEGSFHESMNL
                     AAVWNLPVIFFITNNRYGISTDISYSTKIPHLYQRAAAYGIPGHYVEDGNDVIAVYEK
                     MQEVIEYVRAGNGPAMVEVESYRWFGHSTADAGVYRTKEEVNEWKAKDPLKKYRKYLT
                     ENKIATDEELDAIEAQVAEQVEASVKFAQESPDPDISVAYEDVFVD"
BASE COUNT         5723 a         3290 c         4458 g         5261 t
ORIGIN      
        1 aaatccgatg taacaagctt gttttttaca gaaagcagaa aatgtggtaa aatggagtga
       61 taaaactatt tgaggtaacg aattatgact gttaaaatca atactaaaga cggccaaatc
      121 gaattgactg atgacgtgat tgccacagtt gttggcggtg ctacgactga gattttcggt
      181 gttgttggga tggctagtaa gtctgcgatc aaggataatt ttcaagcact tttgagaaaa
      241 gaaaattatt ctaaaggtgt tgtgattaag accttggaag atggtcgcat cgcagtcgat
      301 gtttatactg tgatgagcta cggtgtaaaa atcagtgaag tatctcgcaa tatccaagaa
      361 cgcgttaagt tcaacttgga aaatcaacta ggtatctctg tagatgccgt caatgtcttt
      421 atccaaaata tcaaagtcgt aggagaataa ttgtgtctaa aattacaact agtttatttc
      481 aagaaatggt gcaggcagca tcaactcgtt tgaataaaca agccgaatat gtgaactcac
      541 tcaatgtttt cccagttcca gatggggata ctgggaccaa catggggatg actattacca
      601 atggtgctaa agaagtagca gataagtctg cttctactgt tggagaagtt gcgcaaattc
      661 tttctaaggg cttgttgatg ggagctcgtg gaaactcagg tgttattacg tcgcaattgt
      721 tccgcggttt tgggcaatct gttaaaggca aggttgaatt ggacggtaag gatttggcgc
      781 aagcctttca acatggtgta gaagtggctt ataaagccgt tatgaagcca gttgaaggta
      841 caattcttac tgtttcgcgt ggggcagcca ctgccgcttt gaaaaaggca gaagagactg
      901 atgatgcggt tgaggtcatg cgtgccactc ttgacggtgc taatcgtgct cttaagaaaa
      961 cgccagagat gcttccagta ttgaaggaag ttggcgttgt cgattcaggt ggacaaggtc
     1021 ttgtttacat ttatgaaggc tttttgtcag ctttgacggg tgagtatatt gcttcggaag
     1081 attttgaggc gacacctgct gttatgtctg aaatgatcaa tgctgagcac cacaagtcag
     1141 ttgcaggtca tgtagcgact gaagatatta cctttggtta ctgtacagaa atcatggtag
     1201 ctcttcgcca ggggcctact tatgtcaagg actttgatta tgaagaattc cgtaactacc
     1261 ttaacgacct tggggattcc ctccttgtgg tcaacgatga tgagatcgtt aaagtccatg
     1321 tccatacaga agatccaggt ttggtcatgc aagcgggtct tcaatatggt agcttggtca
     1381 aggtaaaagt ggacaatatg cgtgagcaac atgaagctca ggttgagaaa gtagaaagtt
     1441 tccaaaaacc ggctgaacaa aaagagtatg ctattattgc ggtagctgct ggggaaggtt
     1501 tgacagaaat cttcaaatca caaggcgttg actatgtgat ttcaggtggt cagaccatga
     1561 acccatcaac agaagatttt gtcaatgcta ttgaattggt caatgcccgc aatgtcatct
     1621 tgttgccaaa caacaaaaat atcttgatgg ctgctcagac ggctgctgag gttgcagaag
     1681 ttgcggttaa ggttgtggaa actaagaccc taccacaagg ttttactagc ttgcttgctt
     1741 ttgatccaag tcgtgatttg gaaagcaact ttgaagcgat gacggcatct ctcgcagagg
     1801 tgaaaagtgg tagcgtgaca actgcggtcc gcgatacaac cattgacggt cttgaaatcc
     1861 atgagaatga caaccttggt atggttgatg gcaagattgt ggtttctaat ccagatatga
     1921 tggagacact tgtggctacc tttgataaaa tgttggatga agacagcgaa atcgtgacta
     1981 tctacatcgg tgaagatggt gatgaggaac tcgctcaaac tcttgctgaa cagctagaag
     2041 aacaatttga agatgttgag gtcgaaatcc atcaaggaaa tcaaccagtc tatccatatc
     2101 ttttcagcgt agaataaaca aaatcgcagg ctctggcttg cgattttttc tgtcgacttg
     2161 tctacaattt ccagtagacc tatgacaaac tactggggct atggtataat taaagacatg
     2221 tatagtcaga atgaaaatga attgattgcc attggtgaga gaattggaaa agcctgtaag
     2281 ccaaatcaag ttctagtatt atcaggggat ttgggtgctg ggaaaacaac tctgaccaag
     2341 ggtttggcca agggtttaaa aattgaacag atgattaaga gtcctactta tacgattgtg
     2401 cgagagtatg aggggaccat gccactctat cacttagatg tttatcgaat tggagatgac
     2461 cctgactcga ttgatttgga tgattttctc tatggaggag gtctaacggt tatcgagtgg
     2521 ggagaattac tggatgtcag tctatttgat gactatttgc tcattcgtat agagaaagag
     2581 ggagatggtc gacgattgac agtcgaatct catggagctc aaagtagcga tttagcgaag
     2641 gagttccaaa atgtctgaaa aagaagtgta ttttagtgaa gctgaaccag ctgatgcagc
     2701 agcttttatt gattttatga atcaagttgc tagagaaacg gattatctgg tcatggatga
     2761 gactggtttt cgatttagtg ttgatgaaat ggagaccatc ttcgaagcag gaatcgaaaa
     2821 tcctcgtgaa ttgtgcctgt tggctaaggt tggttcggaa gtcgtcggcg caatttctgt
     2881 taagtcctct aagcagtttc gcattagcca tatcggaaat atttttattg cagtaaaaaa
     2941 agcctattgg ggtcacggtc tagggacgat tttactggag gaagtattgc attgggcaga
     3001 ggaaatggat gtattgaagc gtttggaatt aacggttcag gttcgcaatc aggcggcagt
     3061 acatttatat caaaaactag gatttaacat tgaaggaacc cagatacgtg gggctagaac
     3121 agatgaagga gaatggcttg atctttatta tatgggcaag ttaattggtg aagtatgaca
     3181 attggaaaaa aaatattctt aatgtctttg gctattattg gtttaacagt aggtgctggc
     3241 ttaatatatg gagccagctt gttaaatttt tctacggatg ctatttcgaa aacatttaag
     3301 cagttagatg gagaagaaaa aatcactccg attgatgcga cagagcccct aactatccta
     3361 ctgatggggg tggatatgga ccaagcaacc cgtggaggag attgggaggg tggtcgtagt
     3421 gattcgatga ttttggtaac tgttaatccc aagactaagg aaaccaatat gatgagcttg
     3481 actcgtgata ttatggttga gattgcagaa gcgaatgggg agtcttcagg aacggttgaa
     3541 aaactaaatc attcttatag ctacggacag gcaccgatgg ccattgccac gatcgaaaag
     3601 atgatggata ttaacattga ccgctatatc gaaataaata tggatggtct ggttgagctt
     3661 gttgatgcgg ttggaggtat tgaggtaaac aatactcttg gcttccctat atccatttcg
     3721 gaacatgagc ctgcctacac ctctattgtc cagccgggga aacagttggt aaatggggat
     3781 caggcgctgg tttatgctcg gatgcgctat gatgatcctg agggggatat tggtcgtcag
     3841 agacgtcaac gtgaagttat aacggctatt ataaaaaaac tccttcagtt agacggtttt
     3901 acccagtata agaagattct gactgccatt tctaataata tgcgaaccaa cattgagatt
     3961 agtcctgcga caattccaaa tctcttgggg tacaaggact ctatctccaa gttgaattcc
     4021 tatcagttgc gcggtgtgga tcaaatggtt gatgcaatct attatcaatt accaaccagt
     4081 acacatctat tggaaatgca aaatgttctg aaaaaatcta tcggcttaga agaaaatacc
     4141 gacttagtca caaacgtgaa ggtctacgaa ggacagatgg gattgccaag tccaataaat
     4201 gtttatgatg tatatacaaa tctcttgctg ttagaagcaa gtccatgggc agaaagcttg
     4261 gatccagaaa tagggagtcc atcggctact acgactgtga caacagaaga gcaggtaacc
     4321 gatttttcaa cagagccagt actcggtgat gaaaacgggt cacaataatg tagtcattca
     4381 gtttttcttt tattactttg acgagcgagt ataaaagcct ataaaaatag aggttgtttg
     4441 caacctctat ttttatatat ttggaaattc cctatgtcag ggagtgttat tctcattctg
     4501 gtattttttg agaatctgtt ggatttcttc caagtttcct gcaatacttt gctgatagac
     4561 tcgagtctta tattgtaagt cagaagccat ttcctgcaag ggttcttgaa aactttggat
     4621 aatggcaagt tgttcctgaa tattgtcctt gttcagttga atatctgata gaagttgttt
     4681 ggtttcctgt acttctttag cgattttatc ccgatgcgtg actgcaagga agctggcggt
     4741 agcggcgctt acggctaaaa tgatattacg aagtttcata ttattctcct agatagagct
     4801 cttgtgccag ttgagccagg gctgggaagt cagggtcgtt ggctttaaat tgaatgctca
     4861 atccgtcttg attgttaaag cgtggtaaga gatggatgtg tgtatgaaag acggtttgcc
     4921 cagcggcctc ctcgttatta ttaacgatgt ttagaccact agctccaagt ttttctttta
     4981 actgacgagc gatggttggc acaacagaaa agagtgtggc tgcagcttct ttatccatgt
     5041 caagcacgtt tcggtagtgt tttttaggga caaccaatgt gtgtcctttt gtggcctgag
     5101 tgatgtctaa aaaggctaat acttggtcat cttcatagac ttttgaagct ggtatttctc
     5161 cagagataat tttgcaaaaa atacaatccg acataatggc aacctctttc tgggattttt
     5221 gttataataa ttatatcaaa ttgtaggaag aaatgtatgt tagaagtaaa aaatgtaact
     5281 ggaggatatg tcaatatccc agttttgaaa gatgtatctt ttaccgttga ggatggtcaa
     5341 ttagttggtc taattggttt aaatggtgca gggaaatcaa cgactattaa ggaaattatc
     5401 ggtcttttga ccccctatca aggacagatt gcaattgacg gcctaacttt gctacaaggg
     5461 gctgaaaatt atcggaagaa gattgggttt atccctgaaa caccaagttt gtatgaagaa
     5521 ctaaccctca aagagcactt ggaagtggta gcaatggcct atgatttatc ctgggagcaa
     5581 gcttggggac gtgttgagca cttgttaaaa ctgtttcgct tggatgagaa attggactgg
     5641 tttcctgtca atttttcaaa gggaatgaag cagaaggtca tgattatttg tgcttttatg
     5701 gtggagccga gtctattgat tgttgatgag ccttttctgg gattagaccc tgtggctatc
     5761 tctgatttaa tcgctctttt gggggaagaa aaatctaagg gcacttcaat cctcatgtcc
     5821 actcacgttc ttgactcagc tgagaaaatg tgcgactctt ttgtgatttt acatcaggga
     5881 caggtccgtg caactggaaa tttggaggag ctacaaagcg catttggtat ggatggtgca
     5941 agtctgaatg aaatttatct ggccttgacg caagaaggag tggctggatg aaagaattgt
     6001 ttcaacaacg ccgactagat tttttaaatc gttgttccaa gtatctgcgc tatgttctga
     6061 atgaccattt tgttttggtt ctcatggttt tattaggctt tttgagtttg caataccgtc
     6121 aattattgtt gtcgttccct gagaatccgt tgccgattgt ttttgtggtt attgcaatca
     6181 gtctactggt actagtgtcc ggaagaattg cgacttatct agaagaagct gatcaaatct
     6241 tcctattgac caaggaaaaa gacattctgc aaggactcga ggttgcagcc agatgcacgg
     6301 tcttgttttg gtcctctata caggtggtgg ttcagcttgt tttgttacca atctatttga
     6361 aactgggttt accggtgtgg atgattgtag ctggtcttct tactttgttg gttttaaaat
     6421 gtctttttgt caagcgccaa ttagtggctt atcagtcaca gggagtattg gactggtcta
     6481 aggctattca agatgaacaa aaaaggaaac agtctatttt acgtttcttt gccttattta
     6541 cgacggttaa aggaatcagt acgactgtta aaccgagaag ctatctcaat ggaatcttgc
     6601 gtcttgtcaa gtcgagacag acttggtttt atctctactt acgggctttc ttgcgttcag
     6661 gagactactt gggcttgacg atacgtctac tgttgttggc gattttgtcc ctatttgcca
     6721 ttgaagaatc gtggttatcc atcggcctag ttttagtctt tcattatttg cttgtctttc
     6781 agttgttggg gctttacaag cattatgatt atcaatatat aacacaactg tatcctctgg
     6841 agaaagattc caagaaaatt ggtcttcaga atttgcttag agtgatttta tatagtctat
     6901 tactggtgga attgggcttt gctctgctat tttctaaaga gaatacgatg gtagtcgtct
     6961 tacttctcgt ggggattttc ttgcatgaaa tatatttgcc tctaaaatta aaaaaattga
     7021 ttgactaaat agacgaaaat tagtaaaata gtaaggaatt tttaaaggag gaaaggcatg
     7081 cagtttgata ccagtgggct acaattacag tctattgcag gaaatagtgg caaagcgttt
     7141 aaaggcctac ggtcagacgg aacgccagtc tttgtgaagt atgagatgcc tccgatagtg
     7201 tctgctcttg cgcgtgagca aattacacca cccgttttgt cagcaaatcg tgaagtagga
     7261 atggggcatc gtgtagagca ggagtggttg aatggtcgta ccttggagcg tcaagatatg
     7321 actagtaaac agattcgtca aatactggtg agaatgcact attctcgcat tttgctcaat
     7381 caggctttgc agatgaatta tacctatatg gaacctcagg atttggttta tcgttggcaa
     7441 gaagaggcgc caactagatt gggacagaat tcttatctgc aatctatctg tcaagattta
     7501 ttgaataatc taccaagatt ccgtcaagag gtggcaacat ttgttcacgg agatttgcat
     7561 catagtaact gggtagaaac gacgtctggt ttggtgtatt tgacagattg ggagaccgct
     7621 tgtgtaacgg atcggatgtt ggatgttgcc tatattttga cgcattatat tcctcgtcag
     7681 tcctgggaag agtggcttcg ggcttatggc tacaaatata acagcacagt attaaataag
     7741 gtttattggt atggccagct tggctatcta aaccaaatag ctaagcatgt agaaagctac
     7801 aatgttttgg ctgcaaataa agaaatttat gctctacggc aattccgtca gagtttctat
     7861 aaggaagtat gagagttaga aatcgtaagg gagcgagtga gcttctagca aacaatcccc
     7921 agtatgttat ctccaatcca gaggagtgta aggggaaatg ggcagagatt tttggaaaca
     7981 ataatcctat ccacatagaa gttggttctg gtaaaggtcg ctttgtcact ggtatggctg
     8041 cgcaaaatcc agatattaac tatattggta ttgatattca gatgaccgtg ttaagctatg
     8101 ccttggatcg tgtgcttgaa gctggcttac caaatataaa attattgcag gtggatggtt
     8161 cgagtttgac caactatttt gcaccggcag aaatagatca gctttatctg aatttttcag
     8221 atccatggcc taagaaacgt catgagaaac gccgtttgac ctacaagtca ttcttggata
     8281 cctacaagga aatcctacct gagaagggag aaatccactt caaaacagat aatcgtggtc
     8341 tgtttgagta tagcctggct agtttttctc aatatggcat ggtcttgaag caagtttggc
     8401 tagatttgca tgctgatggt ttggaaggca acgttatgac agagtatgag gagaaattct
     8461 caaacaaggg tcaagttatc tatcgtgtgg aagcacagtt tcaataattc ccttagtgga
     8521 aaatgaggag aggtctaaca gagacctttt tcctttacaa atagggatag atatgataga
     8581 ataatggaag tgaataatga gaaaaggggt tggcgtcagt caaccctttc cctgtacacg
     8641 gatttctgta tatagaatga atgatagtac aagggagttt actgtcaaag gaaatagaga
     8701 gtcctggata gtgcgaacta tttgagtaat gtatttctag cgactgtaaa aataggaggt
     8761 gtttatgtca tcaattattg aattggtcac agcagcaatt acccccgcta ttcaaactcc
     8821 ttacgagttg gtagatgtgg aatatggtaa aatgggcggt gactatgtcc tatcgatttt
     8881 tgtcgataag gagggtggaa tttcccttca agatacggca gatttatccg aaaagatcag
     8941 tccgattttg gacactatca aaccagaccc atttccagac caatacatgc tggaggtaac
     9001 aagtccaggt ttggaacgtc ctttaaaaac ggcagatgct gttgagaagg cggttggaaa
     9061 gtacatccat gtcaagctct atcaggcaat tgataaaatc aaggtttttg aaggaacgtt
     9121 gctctccttt gatggaacag acctgattat ggagtatatg gataaaactc gtaagaagga
     9181 agtgactatt ccttaccaga cggtggctaa ggcccgtttg gctgttaagt tataatgctc
     9241 tttcatgagt attttaaaag atattagact ttaagaaagg accttttgag cgcaaaagga
     9301 aaacaacatg agtaaagaaa tgctagaagc cttccgtatt ttagaggaag acatgggtat
     9361 taacaaagcg gatatcattg atgcagttac agagtcgctt cgttcagctt ataaacgccg
     9421 ttatggtcaa tcagaatcag cagtgattga atttgacgag aaaaaaggcg atttccatgt
     9481 atttactgtt cgtgaagtag tagatgaagt atttgatagc cgactagaaa ttagtctaaa
     9541 agatgccttg gctatttcat cagcttatga aatgggtgac aaaattaaat tccaagaaga
     9601 cccagcggaa tttggacgtg tagctgctca atcagctaaa cagaccatca tggaaaagat
     9661 gcgcaaacaa aagcgtgcca ttaccttcaa tacctacaag caacatgaaa atgagattat
     9721 gtcaggtacg gtagagcgtt ttgataatcg ctttatctat gtcaaccttg gtacgattga
     9781 agcacaattg tcaaaacaag accaaattcc tggtgaggtt ttccaatctc atgatcgcat
     9841 cgaagtctat gtctacaagg tagaagacaa tggtcgtggt gttaacgtat ttgtcagccg
     9901 tagccatcca gaaatgatca aacgtcttat ggaacaggaa attccagaag tatacgatgg
     9961 aacagttgaa atcatgagtg ttgcccgtga agctggagat cgtacgaaag tggctgttcg
    10021 tagccataat ccaaacgtag atgccatcgg tacgatcgtt ggtcgtggtg gttcaaatat
    10081 caagaaaatc accagcaagt tccacccagc tcgttatgat gctaagaatg accgtatgat
    10141 tcctactgag gaaaatattg acgttatcga gtgggtagca gatgaagcgg aatttatcta
    10201 caatgctctc gctcctgccg aagttgacca agtattgttt gacacggaag atggaaaaca
    10261 tgctacagtc gttgtaccag atgataaatt gtcattggca attggtcgtc gcggtcaaaa
    10321 cgttcgtttg gcagcacatt tgacaggatt ccgtatcgac atcaagtcag catcggagta
    10381 tgaagctttt gaagcaactc aatatgcaac agacgaagtt gtagaagaag tcgcagaaga
    10441 acaagaataa ggcaaaagga gcaaccatgg caaaagctag aaaaataccc ttgcgcaagt
    10501 ctgtggtgtc cggtgaaatc atcgacaaac gggatttgct ccgtattgta aaaaataaag
    10561 aaggtcaagt atttatcgat ccaacaggca aggcaaatgg tcgtggagct tatatcaagc
    10621 ttgacaatga tgaggcaagc caagctaaga aacgtcgtgt cttcgatcgt tcctttagta
    10681 tggaagtggc cgaagaattt tacgatgaat taattgccta tgttgatcat aagatcaaga
    10741 ggagagaact tggactcgaa taagagacag aaaatcttaa atttattagg tttggcccag
    10801 cgagctggtc gcctggtttc gggtgaggac ctggttgttg aagctattca aaaagggcag
    10861 gcaaagttgg tgtttttagc cgaagatgct gctggaaatt taagtaaaaa agtaacagat
    10921 aaaagtcata cctatcaagt agaagttgta acagtgtttt caacactgga attgagtgca
    10981 gctgtcggca aggctagaaa agttcttgca gtgacagatg ctggatttac aaagaagatg
    11041 aggtctatta tggaatagaa caggaggaca aggcattgtc taagaagaga ttgaatgaaa
    11101 tagcccgtga attaggcgta agcagtaagg aagttgttgc taaggctcaa gaattgggtt
    11161 ttgaagtcaa aagtcatgct tcaagcgtag atgaagcaag tgctaaacgc cttgcagaaa
    11221 gctttggagg acaaaaatca gaggcaacta aagttgcggc aaaagttagc aagcctgaga
    11281 aggttgatga aactccaaaa gtggaaacag ctaaggtaga aaaagctaag gaaacacagc
    11341 cagtagtaaa agaagaagtt gcggcaagcg cagtacaatc agttccacat cgtccacaga
    11401 gtcgaaactt taaggcagag cgtgaagcac gggccaaaga gcaggctgct aagcgagctc
    11461 agggtcaagg aaaaggtggt caagctaaat ctggtcagga ccgtcgtgac aaccgtcaac
    11521 aaggtcaggg acgttcaaac aatgagcgaa atgatcgccg cgacaatcgt cgtgaccagc
    11581 gtccagaaga gcggaaagac aaccgatttg gtgaacgccg cgataatcgt gccaaccgtc
    11641 gtcaggacaa tcgttcaggt cggtcagctc gctttgaaca aagagaggct gcaaaaccag
    11701 caggacctaa aattgacttc aaagcgcgtg ctgctgcttt gaaagcagaa caaaatgcgg
    11761 agtatgctcg taccagtgaa gaacgttttc gtcaggctca ggaagctaaa aagcaaccta
    11821 agaaggcaaa agaaattaag tttgaagaac cggttgtgga aagcaaacca tttgtgaagc
    11881 ctgcacctgt tgcatcagta ccagagcaag ttgcagaaac tactgtagat acccgtcgta
    11941 agaaacaagc tcgaccagat aaaaaacgtt actttaatag tgacgaagaa gatggtccac
    12001 ggaaacaaca aagaaatcga aatagtcaaa atcaagtgag aaatcaaaga acaagtaact
    12061 ggaataacaa caagaaaaat aaaaaaggca aggctaacca acctgccaaa cctgtcacag
    12121 aacgcaagtt ccacgaattg ccaactgaat ttgaatacac tgctggcatg accgtcgcag
    12181 aaattgcaaa acgaatcaaa cgtgaacctg ctgaaattgt gaaaaaactc ttccttatgg
    12241 gagttatggc gactcaaaac caatctttgg atggagatac tatcgaactc ctcatggttg
    12301 attatggtat tgaagcgaaa gaaaaggtag aggtcgataa cgcagatatt gagcgtttct
    12361 tcgtagaaga gggctacctc aatgaagaag agatgacaga acgcccacct gttgtgacta
    12421 tcatgggaca cgttgaccat ggtaaaacaa ccttgttgga taccctacgt aattctcgtg
    12481 ttgctactgg ggaagctgga ggtatcacac agcatatcgg tgcctaccaa attgaggaag
    12541 ctggtaagaa aattaccttc ttggatactc caggacacgc ggcctttact tctatgcgtg
    12601 cacgtggtgc atctgttacc gacttgacca tcctagtcgt tgctgcagat gatggtgtca
    12661 tgccacaaac gattgaggct atcaaccact caaaagcagc taatgtacca atcattgtag
    12721 ctatcaacaa gattgacaag ccaggtgcaa acccagagcg tgtcatcggt gaattggctg
    12781 agcacggtgt tatctcaacc gcttggggtg gagaatctga atttgtagaa atctcagcta
    12841 agtttaacca aaacattgat gaattgttgg aaactgttct tttggttgct gaaatccaag
    12901 aactgaaggc agacccaact gttcgtgcga ttggtacagt tatcgaagcg catttggata
    12961 aagggaaagg tgcggttgca accctcctcg ttcaacaagg gacacttaat gttcaagacc
    13021 caatcgttgt cggaaatacc ttcggacgtg ttcgtgccat gacaaacgac cttggtcgtc
    13081 gtgttaaaac tgctggtcca tctacaccga tatctattac tggtttgaac gaagcgccta
    13141 tggcaggcga tcattttgca gtttacgagg atgagaaatc agcccgtgca gcaggtgaag
    13201 aacgtgctaa acgtgctctt cttaaacagc gtcaagcaac gcaacgagtt agtcttgaaa
    13261 acctctttga tacccttaaa gcaggtgagg ttaagtcagt taatgtgatt atcaaggccg
    13321 acgtacaagg ttcagttgaa gctcttgcat catcgcttca aaagattgaa gtagagggag
    13381 tacgtgtcaa tatcgtccac tctgcagttg gtgctatcaa tgaatcagat attactcttg
    13441 cggaagcttc aaatgctctt gtgattggtt tcaacgttcg tccaacagct gaagcgcgta
    13501 gccaagctga agcagatgat gttgaagtac gtcttcacag catcatttac aaggttatcg
    13561 aagagatgga agatgccatg aaaggtatgc ttgatccaga atatgaagaa aaaatcattg
    13621 gtgaggcaat cattcgtgaa accttcaagg tatccaaagt tggaacgatc ggtggtttca
    13681 tggtcgtacg tggtaaggtt acccgtgatt caagtgttcg agttatccgt gatggtgttg
    13741 tagtatttga tggtaaacta gttagtttga aacgctataa agatgacgtg aaagaagttg
    13801 gtaatgccca agagggtggt ttgatgattg aaaactacaa cgacttgaaa gttgatgata
    13861 ccattgaagc ttacatcatg gaagaaatca aaaaataata ttttctcatt gtcaaaatca
    13921 tagacaagtg attttgtcga ggaagtgaca atttaataga ttttagtaga ttttaaagca
    13981 atagttttta atctacttgg attattcaag ctggggaaac tcagcttttc tatcatctaa
    14041 gaaaggaaag aatatggcga atcattttcg tacagaccgt gtcgggatgg aaatcaagcg
    14101 cgaagtcaat gagattttgc aaaagaaagt tcgtgatcca cgtgtgcagg gagtgaccat
    14161 tacagatgtt cagatggttg gcgatttgtc aatggccaag gtttactaca caatcatgag
    14221 taatttggca tccgataatc aaaaagcaca aacgggtctt gaaaaagcga cgggaaccat
    14281 taagagagaa cttggtcgta agttaactct ttataaaatt ccggatttgg tctttgaaaa
    14341 agaccaatca attgagtacg gcaataaaat tgaccaaatg ttgcgtgctc ttgatcagaa
    14401 agattaacaa caaaagtcgg atggaaacat tcggcttttt ctggctcgga tgctttgtcc
    14461 atatatggtg gcgtttacaa gccctcggaa agatgttata ctaatactca atgaaaatca
    14521 aaagtagact aggaaacgaa gtcgaagata gaactctgtc actatcttat ttcaaggtaa
    14581 caggctgaaa acctccactg gagcttttca ctcatcaagg caagttgaca acggataatt
    14641 ttgattttcg aagagtataa gaccaaagat agagcacagg aggaagttat gaaaacattt
    14701 attagtaaac tgatgttagt gtccacggtt gtactattag ctgcttgtca gtctaatcag
    14761 gtaaaaggtg gtgagagttc tagttctagt gcttcaagcc aagtttcaga tggttctagt
    14821 ttgcagcaag catcaacaat agcatctagc tctgagacgc aagcatcctc agtgaccact
    14881 cctgagctaa gtgaagtata ccaatcagtt attgatcgtt atcaagcgaa tatggggcag
    14941 tctgctgagg caattaatca ggatgaggtc agtagctatc tgagcttatt aactagccag
    15001 gggcaagaat acagtggaat attttatagt ttgtatgatg tcaaccatga tggtacagag
    15061 gagctgcttc tagctttgga caagtcggga gaatatgtct taattgatct ctatactcag
    15121 cttgccgatg agagtctacg tttggtggat aatttccgca atcttggatt tgaaattgga
    15181 ccgaacgccc tacttcgtcc tttacaggat ggaacatact tatttgaagg agatggtatt
    15241 tttcgcattt atcaatacaa tgcgatgatt ccaggtttaa aacggatttc tgaatcagat
    15301 accaatcctg aaacttctcc cttgcttgag ttgaaaagct tgcattgggt taagctatag
    15361 attttatcaa tgattattcc taggctgggt ttcccagcct tttgtagttt ctaagaactt
    15421 taagttttct tttggtaatc tttaatcttt cttttaggaa ggaaaggtaa actaggagca
    15481 tatcaggaag ggatacgact tcctaaagaa agaaaaggaa tctacattat gaaaacaaat
    15541 cttaaaaaat tactttactc tggtgttact cttatgagcc ttggtgtctt agcagcatgt
    15601 tcatctactt caagttcaac gactacttca agctcagcag caactagtca gacaagtgta
    15661 gcgacaactt caaataatag cacttcatca gattcaagct cgtcaactat tgactggtca
    15721 gcccttccaa cgacagaagt gacgctttca aatgatggct tgaaaattac tgagggtgga
    15781 acctatattt tgactggttc aacaacagct ggtgtaacgg ttgaaacaga tgccaatgtt
    15841 cgcatcattc tagcaggtgc tgagatttca agttcagata cagcagcaat caatgttatt
    15901 aatgctgaca atgttgaatt ggaaatcaaa gatggtacga cgaatacagt gaaagatacc
    15961 agcaatcata cagacaccaa tattgaaggg gctatccatg ttgaagctga tttgaccatt
    16021 acaggaaatg gtagcctgac cgttgaaggt aacttccaag acggcatcgt atcgacggac
    16081 gaccttgtcg tgaattcagg aaacattacc gtcactgcag tggatgatgg tatccgtggt
    16141 aaggattcgt tgacaatcaa tggtggtgaa attactgtaa cagcaggtga tgatggtatt
    16201 aaagcggaga cggctttgac aattgacgga ggcaccatta cagtatcaga atcagcagaa
    16261 gctcttgaag gaacgaatat aaccatcaat ggtggtacat tagatgttta cggttcagat
    16321 gatgctatta atgcggcaag tacaacgtca tcagaaatct tcatcaaggt aactggcggt
    16381 gacttgaaag tagctgtagg tagtggagat acggatgcct ttgatgctaa tggagacatt
    16441 tatatatctg gtggtacaat cgatgtaaca gctcaatctg cttttgactt tgacggaact
    16501 gctgaattga caggaggtac tgtgacagtt aatggtgagc agattactca aatcacagca
    16561 acaggaccag gtgctggtgg ccacggtggc tggtaatcaa taataataca aaaaggtctg
    16621 agttgacgaa ctcagacctt tcgtttgctt tccatattgg gaagattgat tttgtattta
    16681 attaccaata atcgaatgat taaagaaatg gtaaaaatag tgatgaaggt tggaatttgt
    16741 ggtaatttcc aatttacaac gcaaagatga tagaggactc ctgagaaaaa tgcaaggaca
    16801 gcatagacat cttctttaag aacaatgggt gtttcattga cgagtaaatc acgggcgatt
    16861 ccgcctccga cacctgtcaa agctgccaga ataccactgg ttagagcgtt tagctggaga
    16921 gagatgccag tactggctcc aatgagggcg aagatggcta gaccgattgc atcaaagata
    16981 aggtttgtct gggaaaggaa atgaatcatc ttcttatcaa gtggtttgtc ctgctttaga
    17041 ttgataacaa tcaagtacat gctaatcgat actccgagag ataaatagat ggctgtagga
    17101 tcagttaagg cggcaggaat gcgattggcc atcgtatctc gaataattcc cccaccaacg
    17161 gcggtaatga tgccgagcaa gctaattcca aaaatatcta actttttctt aaatcctttt
    17221 acggcccccg aaacagcaaa tgcgatagtg cctatgtagt tacaaatcat aagaaataaa
    17281 tcgaattcca taagaacctc cgtacctata ttaacagaaa gaaagatggc gtgcaagctt
    17341 attttaaagt ttgtgaaaaa agtttcaaac aagtaaagtg aatcttttaa tgatttgtga
    17401 aaaaacgcac taaatcaagg ttttcacaaa aatttctgta aaaagaaaac ggttcaatta
    17461 cttgctattt gtgaaatatt ggtctatcat agtacagaaa gagaaaaacc tttggaggaa
    17521 attatggtat ctatcacaaa agaacaacat ttggacatgt tcttgaaaat gcaacaaatc
    17581 cgtgatgttg atatgaaatt aaacaaatta gtgcgtcgcg gtttcgtaca aggtatgact
    17641 cacttctcag tcggtgaaga agctgcggct gttggaccta ttgctggatt gacagatgag
    17701 gacatcatct tctcacacca ccgtggtcac ggtcacgtta ttgcaaaagg tattgacatc
    17761 aacggtatga tggctgagct tgctggtaag gcaactggta catcaaaagg ccgtggtggt
    17821 tctatgcact tggccaatgt tgaaaaagga aactttggtt caaacggtat cgtaggtggt
    17881 ggttatgccc ttgcggtggg tgcggccctt acgcaacaat acctcggtac agataacatt
    17941 gttattgcct tctcaggtga ttcggctact aacgaaggtt cattccacga atcaatgaac
    18001 ttggcagctg tttggaactt gccagttatc ttcttcatta caaacaaccg ttacggtatt
    18061 tcaactgaca tttcttattc tactaagatt cctcacctct accaacgtgc agcagcatac
    18121 ggtattccag gtcattatgt tgaggatggt aacgacgtta ttgctgttta tgaaaaaatg
    18181 caagaagtca tcgagtacgt tcgtgcaggt aacggtccag ccatggtcga agttgaatcg
    18241 taccgctggt ttggtcactc aactgccgat gcaggtgttt accgtactaa agaagaagtc
    18301 aatgagtgga aagcaaaaga cccacttaag aaataccgca aatacttgac agagaacaag
    18361 attgcgacag acgaagaatt ggatgccatc gaagcacaag ttgcagaaca agtagaagca
    18421 tctgtgaaat tcgcacaaga aagcccagat ccagacatct cagtagctta tgaggatgtg
    18481 tttgtagact agggtgtgag gacgttaaag aggcacagaa aaattaggga gtcagctagt
    18541 gatgtggaca tcactaatgg ctcatctatt tttccaagcc tctagtcaga acctaactac
    18601 aaacgtaaga agtttgacta agctctaaat agcaagtcaa actagggtgg actacctact
    18661 caagttcgag tacaattcta aggtgtctta agctgggaca ataaacaatt acatcgtagt
    18721 aattgtttgg tt
//
LOCUS       DBNFEG010000021        18593 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.021, whole genome shotgun sequence.
ACCESSION   DBNFEG010000021 DBNFEG010000000
VERSION     DBNFEG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18593
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.021"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(130..2463)
                     /locus_tag="AC1PNG_000515"
     CDS             complement(130..2463)
                     /locus_tag="AC1PNG_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIP6701046.1"
                     /translation="MNNKIIETLEFHKVRQKIEPYLLTEQGFEELRQLEPMVEVQRIQ
                     QAFDELTDIAQIFVENPYFSLSATSDIGPAMRRLELDTDLNIAELLAVKKVLEVSKSL
                     LDFYGNLENVSLSQLDKLFEKIELFPHLQGSLQSINDAGFVEDFASEKLARIRRKIRE
                     AEDQVRQVMQDILKTKGDMLSDSILASRNGRNVLPVKNTYRNKIAGVVHDISASGSTV
                     YIEPRAVVTLNEEISHLRAEERHELNRILQELSDMLRPHSGVIRNNAWLIGHIDFVRA
                     KHLFAREHQAVVPKLSEKQDIALLNVRHPLIAKPVPNDLYFGSQLTAIVITGPNTGGK
                     TIMLKTLGLTHLMAQSGLPILADKGSRVAIFKEIFADIGDEQSIEQSLSTFSSHMTHT
                     VQILAEADQDSLILFDELGAGTDPQEGASLAMAILDDLRLRGIKTMATTHYPELKAYG
                     IETSGIENASMEFDTNSLRPTYKFMQGVPGRSNAFEIARRLGLSDIIIQSAQSWTDTD
                     SDVNRIIEKLESQTVESRQRLDKIRDVEQENYKMNRALRKLYDELNRERENELNKARL
                     EAKEIVDMALAESEDILKNLHAAASLKPHQIIEAKAELKKLAPEVVDLSKNKVLKKAK
                     IQREAKVGDDIIVTAYGQRGTLTNQLKDGRWEAQVGLIKMTLAKDEFELVKAEKAEQP
                     KKRQVHTVKRANVRGPKARLDLRGKRYEEAMMELDEFIDQALLNNLAQVDIVHGIGTG
                     VIREGVTKYLRRNKQVKEFGYAPQNAGGSGCTIVTFK"
     gene            complement(3014..3562)
                     /locus_tag="AC1PNG_000516"
     CDS             complement(3014..3562)
                     /locus_tag="AC1PNG_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIP6701047.1"
                     /translation="MISLAIILILAWSFYIGYSRGLVLQAFYSMGSIIALVVATATYK
                     KLASFLYLWVPFANATQGSSNYYFDEKYLFDLDMVFYAGLAFLLIYVLVYAIVRFIGI
                     FVHLLEAFNPDTKTTNLISGALAVLVTFISLQIVMVLLSTIPLAMIQDKLHSSFLANI
                     MIQYTPFTSSFLKSLWLSNIAG"
     gene            complement(3559..3870)
                     /locus_tag="AC1PNG_000517"
     CDS             complement(3559..3870)
                     /locus_tag="AC1PNG_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701048.1"
                     /translation="MANLNRYKFTFGEKQLTLTTEHDNLFMEEIERIAQEKYQAIKEK
                     MPTADPETLALLLAINALSVQLTREMAFEQKEQELASVKEKVLKKNVTLIDLDELEDK
                     V"
     gene            complement(3928..4641)
                     /gene="treR"
                     /locus_tag="AC1PNG_000518"
     CDS             complement(3928..4641)
                     /gene="treR"
                     /locus_tag="AC1PNG_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HIP6701049.1"
                     /translation="MKKYQEIYNDLKEKIRTNVYPAESSLPTEQQLQEIYGVSRDTVR
                     KALAILTEGGLIQKVQGRGSMVLKQEILNFPVSGLTSYQELTNVLQLSTKTDVVSLDM
                     ITVNSSLSHLTGFEPYSKVWKVVRTRSIDGKVSVVDTDYLAVDVVPELTTAIAEKSIY
                     EYLENKLGLDIAYAQKEITVEPTNREERELMQSKDDYLVLIKSRVYLGDTQQFQYTES
                     KHKIDKFRFVDFARRKHSL"
     gene            4874..6868
                     /gene="treP"
                     /locus_tag="AC1PNG_000519"
     CDS             4874..6868
                     /gene="treP"
                     /locus_tag="AC1PNG_000519"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HIP6701050.1"
                     /translation="MGKFEKEAKQLLEAIGGKENVNAVTHCATRMRFVLADEKKANVK
                     VIEDIPAVKGTFTNAGQFQVIIGNDIPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNAI
                     QRVMTMLAEIFTPIIPALIVGGLILGFRNVLEGVQMEWLGQAMKDGQLVFDADGNPVW
                     NTIVQVSPFWNGVNHFLWLPGEAIFHFLPVGITWSVSRKMGTSQILGIVLGICLVSPQ
                     LLNAYAVPGTDAATIASDWSWNFGAFSIARIGYQAQVIPALLAGLALSYLEIFWRKVV
                     PEVVSMIFVPFLSLLPAIILAHTVLGPIGWTIGQWLSTVVLAGLTGPLKWLFGAIFGA
                     LYAPFVITGLHHMTNAIDTQLIADAGGTGLWPMIALSNIAQGSAVFAYFLMNRHNEKE
                     AQVSLPATISAYLGVTEPALFGVNVKYVYPFVAAMIGSSIAGLLSVTFNIQAASIGIG
                     GLPGILSIKAEYWGAFFMTMIVAIVVPMILTAVFKRTGAFSKKEETVEEVVAPTEAVV
                     ETGAAIELISPLTGQTKELSQATDPVFASGVMGQGVLIDPTEGLLVAPVDGEISVLFP
                     TNHAVGITATNGVEFLMHIGMDTVGLEGKGFTAHVKQGDKVKAGDKLISFDIDVIKAA
                     GLVAETPVIVTNQTDFDTQVIGNLPRAISQGEAILTVTKN"
     gene            6949..8574
                     /gene="treC"
                     /locus_tag="AC1PNG_000520"
     CDS             6949..8574
                     /gene="treC"
                     /locus_tag="AC1PNG_000520"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HIP6701051.1"
                     /translation="MTIDKRKVVYQIYPKSYKDTTGNGVGDLRGIIEKLPYLKELGID
                     MIWLNPFYPSPQRDNGYDISDYTAVNPDFGTMADFEEMVTVGKELGIEFMLDMVLNHC
                     STDHEWFQKALAGDKYYQDFFILRDQPTDWVSKFGGNAWAPFGDTGKYYLHLFDVTQA
                     DLNWRNPHVREELFKVVNFWKDKGVKGFRFDVINLIGKDEVLEDCPINDGKPAYTDRP
                     ITHDYLKMMNNATFGAEKGFMTVGEMSATTIENCILYTAPEREELSMAFNFHHLKVDY
                     KDGQKWTIMDFDFEELKQLFHTWGEEMSAGNGWNALFYNNHDQPRALNRFVDVENFRK
                     EGATMLAASIHLSRGTPYVYMGEEIGMIDPDYDSMDDYVDVESINAYQMLLDQGHAPE
                     QAFKIIQAKSRDNSRTPMQWDASDNAGFSTGTPWLKAGKSYPTINVEAEKTGPIFTFY
                     QELIRLRKELPLISEGDYKAAYKDSQKVYAFERLLNGEKLLVLNNFFAEEVELDLADD
                     YAHGQVLISNYPDNKLGKKITLKPYQALAIQVK"
     gene            complement(8987..10087)
                     /locus_tag="AC1PNG_000521"
     CDS             complement(8987..10087)
                     /locus_tag="AC1PNG_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIP6701052.1"
                     /translation="MKNNKKMLYTSSLALSLFSTGMISTNVLAIEWAPRTVSEISPEI
                     VQEEGKMTYTVQYGDTLSAIALAMNIDMDLLAKINQIADVNLIFPDTVLTTTVDQNNQ
                     VTQVEIEAPVQGNTNETVQAIVDLTTNQVTVEDTVVPLDQISSVTDSAPVEEVVEQPV
                     AEASVEEVVEQPVVEAPVEEVVEQPVAEASVEEVVEQPVVEAPVEEVVEQPVVEAPVE
                     EVVEQPVVEAPVEQPVVETPQVTALSTTTTSTSAYDVGLQPQVAAFRAEVANAFGITS
                     FSGYRPGDSGDHGKGLAIDFMVPESSALGDQVAAYAVANLASKNINYIIWKQRFYAPY
                     DSIYGPAYTWNLMPDRGSVTENHYDHVHVSFN"
     gene            complement(10496..11479)
                     /locus_tag="AC1PNG_000522"
     CDS             complement(10496..11479)
                     /locus_tag="AC1PNG_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIP6701053.1"
                     /translation="MEVQMKYKWATLGTGVIANELVQALQAMVGNLYSVANRTYDKGV
                     EFAQKYGIEKVYREIDEVFEDPEVDIIYISTPHNTHINYLRKALKAGKHVLCEKSITL
                     NSEELAEAIQLAEENQVILVEAMTIFHMPIYRQLSEVVASGKLGDLKMIQMNFGSYKE
                     YDMTNRFFNKNLAGGALLDIGVYALSFVRWFMTEKPNQVLSQVKLAPTGVDEQVGILL
                     SNDAGEMATIALTLHAKQPKRGTIAYDKGYIELYEYPRGQKAVITYTEDGSQEVIEAG
                     DTAKALSYEVADMEKAVAGIENTMHLAYTQDVMEIMTQLRKEWGLVYPEEM"
     gene            11564..12454
                     /gene="rnhC"
                     /locus_tag="AC1PNG_000523"
     CDS             11564..12454
                     /gene="rnhC"
                     /locus_tag="AC1PNG_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HIP6701054.1"
                     /translation="MNTVVLKASAQQKQAMMAHYDRYRQTSKNPYIEAFFKLTGASLS
                     IYTSGKVVFQGEMAEQEAGLWGYEPESSGQTTVPGQNLPMIGTDEVGNGSYFGGLAVV
                     ASFVRPEDHAFLKSLGVDDSKKMTDQKICQVAPLLKEKIPHQALLLSPKKYNEVIEQG
                     YNAVSVKVALHNQAIFLLLQKGVQPEKIVIDAFTSSQNYQKYLKKEANQFANPVTLEE
                     KAEGKYLAVAVSSIIARAMFLENLVQLGQLVGMNLPSGAGSKSDQVAASILKQYGMAG
                     LNQTAKLHFANTQKAQKLLK"
     gene            12464..13093
                     /gene="lepB"
                     /locus_tag="AC1PNG_000524"
     CDS             12464..13093
                     /gene="lepB"
                     /locus_tag="AC1PNG_000524"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIP6701055.1"
                     /translation="MGAIQKTKRSPLVAFLAEWGIFLLFMAAFFASRYFIWNPVSVDG
                     HSMDPTLQHQEKLIMLKTTSIDRFDIVVASETDSDGKEKLIVKRVIGMPGDTIRYEND
                     VLYVNDQKVDEPYLDEYLAAFQKDKLQEVYSYNKQFQAVAQSAEAFTQDANGYVDFTV
                     TVPEGQYYLMGDDRLVSLDSRSVGTFSRENIKGEVVFRMWPLNRIGTVD"
     gene            13157..15646
                     /gene="recD2"
                     /locus_tag="AC1PNG_000525"
     CDS             13157..15646
                     /gene="recD2"
                     /locus_tag="AC1PNG_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SF1B family DNA helicase RecD2"
                     /protein_id="HIP6701056.1"
                     /translation="MNEVYFTGTIDRIIFENPSNFYKILLLEIEETDADYDDYEIIVT
                     GTIADVIEGEDYRFYGNLVTHPKYGQQLQISRYERSKPTSAGLVKYFSSEHFKGIGRK
                     TAEKIVELYGEDTIDKILAEPEKLTQITGLSSKNMQAFVEKLRLNYGTELILAKLAEY
                     GIPNKLAFQIQDQYKEKTLQIIEENPYQLVEDVQGLGFTIADRIAENLGIASDSPQRF
                     RAGMLFSLIHRSMETGDTYVEARDLLEATLELLEKSRHTELDPAAVAKELTGLIADDK
                     VQQEGTKIFDNSLYFAEHGIHKNLTRLMGKNGFKPFPRTDVEAAIAELETMSSLTYDD
                     IQKEAIVQAITNPLFILTGGPGTGKTTVINGIIAVYAILHKIDLTRNREECPVLLAAP
                     TGRAARRMNELTGLPSATIHRHLGLVEGQEESYRDDYLDADFIIVDEFSMVDTWLANQ
                     LFQNISSQTQVLIVGDAEQLPSVSPGQVLADLLKIDKLPSITLERIYRQSDDSTIVTL
                     ASQIRQGALPSDFREKKADRSYFEAQNEQIPALIERIVGAAIKSGIPANEVQILAPMY
                     RGAAGIDQLNTMTQALLNPLEEGELEFLHNEQAFRQGDRVIHLVNDAEANVFNGDLGY
                     ITDLLPAKYTDSKQDEITINFDGSEVTYPRNEWYKITLAYAMSIHKSQGSEFQVVILP
                     ITRTSHRMLQRNLVYTAITRSKSKLILLGEISAFDYAVKNAGTVRKTYLVPRFQGEMA
                     EQESKESLIKKDESKTATTTQPQPPTQPDRKEQVKVVKENNQQLSLLDQDQPKKTSPQ
                     PTEYILTVDNLLTIDPMIGIEQADIEEFFKA"
     gene            complement(15685..17746)
                     /locus_tag="AC1PNG_000526"
                     /pseudo
     CDS             complement(15685..17746)
                     /locus_tag="AC1PNG_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636049.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            complement(17919..>18593)
                     /locus_tag="AC1PNG_000527"
     CDS             complement(17919..>18593)
                     /locus_tag="AC1PNG_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HIP6701057.1"
                     /translation="EKNEKTVIICPVGPVGQYPHFVRLVNQFKIDLSNVWFINMDEYL
                     LEDLHWIDVVHPLSFRGFMQKNVYSQIHPTLIMSEEQRIFPDPLDIDSISNKIKELGK
                     IDLCIGGIGLNGHIAFNEPDSTLTVQEFLKLGSRVLPISVETKVMNGLTVLKGAVEEV
                     PNYCVTIGMSEIFQAKKIRLAVFRDWHHAVIRKTVCSKPDTGFPSTLMQIHTNCSIYL
                     NESLAN"
BASE COUNT         5266 a         4363 c         3779 g         5185 t
ORIGIN      
        1 tatttcttac ctcataagta tagtatagca taacctagta agtacttgaa ctgtattttg
       61 acctatccta ttccctgtat aacagaaata taaaagcaag aacagactga atctatcctt
      121 gctaattcct tatttaaacg tcacaatagt acagcctgat ccacctgcat tttgtggggc
      181 atagccgaac tccttgacct gcttgtttcg gcgtaggtac ttggtcaccc cttctcggat
      241 gacacctgta cctataccgt ggacaatatc gacttgggct aggttattga gaagggcctg
      301 atcgataaat tcatccagct ccatcatagc ctcttcgtag cgtttgccac ggagatctaa
      361 gcgggctttt ggtcctcgta cattggcacg tttgactgtg tgaacctggc gtttcttagg
      421 ctgctctgcc ttctccgcct tgactaactc aaactcatct ttggccaagg tcatcttaat
      481 caagccaacc tgagcttccc aacgcccgtc cttgagctga ttggtcaagg tcccgcgttg
      541 tccataagct gtaacgataa tgtcatcgcc caccttggct tccctctgaa ttttagcttt
      601 cttcagaacc ttgtttttag acagatccac cacttcaggc gccaactttt tcagctctgc
      661 cttggcttcg ataatctggt ggggcttaag actagctgca gcatggagat ttttgagaat
      721 atcctcgctt tctgccaatg ccatatctac aatttccttg gcttccaagc gtgccttgtt
      781 gagctcattt tccctctcac gattgagctc gtcatagagc ttgcggaggg ctcggttcat
      841 cttgtaattt tcttgctcca catcacgaat cttatccaga cgctgacgac tttcaaccgt
      901 ctgactttcc aatttctcga taatgcggtt cacatcgcta tctgtatccg tccaagattg
      961 ggctgactgg ataataatat cagatagacc aagacggcga gcaatttcaa aggcattgga
     1021 gcgaccagga acaccctgca taaacttata ggtcggacgc aggctattgg tatcaaactc
     1081 catgctggca ttttcaatac cagaggtttc tatcccgtag gccttaagct cagggtagtg
     1141 ggtggtcgcc atggtcttga tcccccgcag acgaaggtca tccaaaatag ccattgccaa
     1201 ggacgcaccc tcctgcggat ctgtcccagc tcccaactca tcaaatagaa tgagggaatc
     1261 ttgatccgcc tccgccaaaa tctgtaccgt gtgggtcatg tgactggaga aggtcgataa
     1321 actctgctcg atggactgtt cgtctccaat gtccgcaaaa atttctttga agatagctac
     1381 ccgactgccc ttgtcagcca agataggcaa accagactgg gccatcagat gggtcaatcc
     1441 caaggtctta agcatgatgg tcttaccacc tgtgttggga cctgttatca cgatagccgt
     1501 caactgacta ccgaaataaa ggtcatttgg tacaggctta gcaatgagcg gatgtcgaac
     1561 gttgagaaga gcaatatcct gcttctcaga tagtttgggt acaactgctt gatgttcacg
     1621 tgcaaagaga tgcttagcac ggacgaaatc aatatgcccg ataagccagg cattgttacg
     1681 gatcacacca ctatgcggac ggagcatatc cgataattcc tgcaggatac ggttgagctc
     1741 atggcgttct tctgcacgta ggtgactgat ttcttcgttc aaggtcacca ctgcccgcgg
     1801 ctcaatataa accgtcgagc cagaagcgga gatatcatgg actacacccg caatcttatt
     1861 gcgataggta tttttaacag gaaggacatt gcgtccatta cggcttgcca aaatactatc
     1921 ggacagcatg tcgcccttgg tcttcaaaat atcctgcatg acctgacgaa cttgatcttc
     1981 agcttcacgg atttttcttc taatacgagc cagcttttct gaggcaaaat cctctacaaa
     2041 accagcatca ttgatggact ggagggagcc ctgtaaatgt ggaaaaagct caatcttctc
     2101 aaagagttta tccagctggc taagactgac attttccaga tttccataaa aatccaagag
     2161 agatttagaa acttccaaga cctttttaac agctaataat tcagcgatat tgaggtctgt
     2221 atccaattcc aaacgacgca tggctggacc aatgtcacta gtagctgaca gactgaaata
     2281 gggattttcc acaaaaatct gcgctatgtc tgtcaactcg tcgaaggcct gttggatacg
     2341 ttggacttcc accatgggct ccaactgtcg taattcttca aagccctgtt ccgtcaagag
     2401 atagggctca attttttgtc ttaccttgtg aaattcaagg gtttcaataa ttttattatt
     2461 catatgttcc tacttagtct tttccttagc tttatctcat tatcaaaaca ataaaagata
     2521 aaaatatagt ctttcccaat ctcttacatt aaagttataa cagtcaacta atactcaatg
     2581 aaaatcaaaa tcagacttgc tccaagggtt cgtgggaacc tttagaggtt ggaaataggc
     2641 gaacgtagtt cgtttcgctc tgatgcagat agaactctgt tccttttata tgtcaaggga
     2701 acaggctgag gctgggcaga aagtccatcc tcgcttctca gaattcgtgt caacatctca
     2761 gcgcagtggt tgattggcag atttgttcgt gttccacact ccaaatccga cctaatcaac
     2821 tgtgcggggg tgggagtgaa acagtctggg gatagactgt ttcagctcaa caacttaaaa
     2881 cgaaaaattg ttgacgaact cttttagact gctcgagttc tttcccactc ccactcatca
     2941 aatcaagtca acaatgtctg attttgattt tcgaagagta tcataaatga cgataaaaag
     3001 gataaggtct cccttaccct gctatattac tcaaccagag agattttaaa aagctactag
     3061 tgaatggtgt gtactgaatc atgatgttgg caagaaaact actgtgcaat ttatcttgaa
     3121 tcatcgctaa cggaatagtt gatagcaaga ccatgacaat ctgaagagaa ataaaagtca
     3181 ccaagacagc caaggcgccg cttatgagat tggtcgtttt tgtatcagga ttaaaagcct
     3241 ctaatagatg gacaaatatt cctatgaaac gtacaatagc ataaaccaag acgtatatca
     3301 aaagaaaggc caatcccgca taaaatacca tatctaggtc aaagagatat ttctcatcaa
     3361 aataatagtt ggagctcccc tgagtagcat tggcaaatgg cacccagaga taaaggaagg
     3421 atgccaattt tttataagta gccgttgcaa ccaccagagc aataatactt cccatgctat
     3481 aaaacgcctg tagaactaga ccacggctgt agccaatgta gaaactccat gccaaaatga
     3541 gaataattgc taatgaaatc ataccttgtc ctcaagctca tctaagtcaa tcaaggtcac
     3601 attctttttc aagacctttt ctttgacgct tgcaagttct tgttcctttt gttcaaatgc
     3661 catctcgcgc gttagttgaa cggatagagc attgattgcc aacaaaagcg cgagggtttc
     3721 agggtccgcc gtcggcatct tttctttgat ggcttggtat ttttcctgtg caatacgctc
     3781 aatctcttcc atgaataaat tatcatgctc agttgtcaaa gttaattgct tctcgccaaa
     3841 tgtaaattta tatcgattta agtttgccat atcttacctc ttcttctatc tattatagcg
     3901 taaaaaactt gatttggcta atctctccta taaagaatgc tttctacgag caaaatctac
     3961 aaagcggaat ttatcaattt tatgcttgct ttctgtatat tggaattgtt gggtatcacc
     4021 gagatagaca cgagatttaa tcaagaccaa ataatcatcc ttcgattgca ttagctcacg
     4081 ctcttctcga ttggtcggct ctaccgtaat ctctttttgt gcataagcaa tatcaaggcc
     4141 taacttattt tctaggtatt cgtagatgga tttttcagca atagcagttg tcaactctgg
     4201 cacgacatcg acagcaagat aatctgtatc cacaacggag accttaccgt caattgaacg
     4261 tgtacggaca actttccaca ccttgctata cggctcaaag cctgtcaagt gcgaaaggct
     4321 actattaacg gtaatcatat ctaagctgac aacatctgtc ttggtagaaa gctggagaac
     4381 atttgttaat tcctgatagg aagttaaacc tgaaactggg aaattgagaa tttcttgctt
     4441 aaggaccatt gaaccacgcc cttgcacttt ttgaatcaaa cctccctcag tcaaaatcgc
     4501 caacgcctta cgaaccgtat cacgactaac accatagatt tcctgaagct gctgttctgt
     4561 cggtagggag ctttctgccg gataaacatt tgtccgtatt ttttctttta agtcattata
     4621 aatttcttgg tattttttca tcatttcctc gcaaattcat ctgtcaaata acatacaact
     4681 atcaacatta taacaaaatt catttcaaaa ttaaaaaaac taagcaaaaa cgcttgcatt
     4741 attttttgaa atcggataca atgataacac aaaagaaatt tgtccgtaca aatttcaaaa
     4801 aagacatact tttgggagat gtctaaaaaa tatagggtga cggattccat aagccgtcta
     4861 gaaggaaaaa tatatgggaa aatttgaaaa agaagccaaa cagctcctag aagctattgg
     4921 cggaaaagag aatgtcaatg cagtaacaca ctgtgcaaca cgcatgcgct ttgttcttgc
     4981 agatgagaaa aaagcaaacg taaaagtcat tgaagacatt ccagcagtaa aaggaacatt
     5041 tacaaacgca ggtcaattcc aagtcatcat cggtaacgat ataccaatct tctacaatga
     5101 ctttacagct gtgtctggta tcgaaggcgt atcaaaagaa gctgcaaagt cagccgctaa
     5161 aagtaaccaa aatgccatcc aacgtgtcat gaccatgctg gcggaaatct tcacaccgat
     5221 cattcctgcc cttatcgtcg gcggtttgat tctcggtttc cgtaacgtct tggaaggtgt
     5281 tcagatggaa tggcttggac aagctatgaa agatgggcag ttggtcttcg atgctgatgg
     5341 taaccctgta tggaacacca tcgttcaggt ttctcctttc tggaatggtg tcaatcactt
     5401 cctctggttg ccaggtgaag ccatcttcca cttcttgcca gtaggtatca cttggtccgt
     5461 atctcgtaaa atgggaactt cacaaatcct tggtatcgtc cttggtatct gtttggtttc
     5521 tccacagttg ctcaacgctt atgctgtacc tggtacagat gcagcaacca ttgccagcga
     5581 ctggtcttgg aacttcggtg catttagtat tgcccgtatc ggttatcaag cacaagttat
     5641 ccctgccctc cttgctggtt tggcactgtc ttacctagaa atcttctggc gtaaggttgt
     5701 tccagaagtg gtctcaatga ttttcgtacc attcctatca cttcttccag caattatctt
     5761 ggctcacact gtccttggtc ctatcggttg gacaatcggg caatggttgt caacagttgt
     5821 attagctggt ttgactggtc cattgaaatg gctcttcggt gcgatattcg gtgccctcta
     5881 tgcaccattc gttatcactg gtcttcacca catgacaaat gccatcgata cacaattgat
     5941 tgcggatgct ggtggaactg gtctctggcc aatgattgcc ctttctaaca ttgctcaagg
     6001 ttcagcagta tttgcctact tcctcatgaa ccgtcacaat gaaaaagaag cgcaagtttc
     6061 tcttcctgca actatttctg cttatcttgg tgttactgaa ccagccctct ttggtgtcaa
     6121 cgtgaaatac gtttacccat tcgtagctgc tatgattggt tcaagtatcg caggcttact
     6181 ctctgtaacc ttcaacatcc aagcagcttc tatcggtatc ggtggtcttc caggtatctt
     6241 gtctatcaaa gctgaatact ggggtgcatt ctttatgaca atgattgttg cgattgttgt
     6301 cccaatgatt ttgacagctg ttttcaaacg tacaggtgca ttttcaaaaa aagaagaaac
     6361 tgttgaagaa gttgtagctc caactgaagc agttgttgaa actggcgctg ctatcgaatt
     6421 gattagccca cttactggtc aaaccaaaga attgtcacaa gctactgacc ctgtatttgc
     6481 atcaggtgtc atgggccaag gtgtccttat tgacccaacg gaaggtcttc tggtagcacc
     6541 tgttgacgga gaaatttctg tcctctttcc aactaaccat gctgttggta tcactgctac
     6601 aaacggagtt gagttcctta tgcacattgg tatggatact gttggattgg aaggtaaagg
     6661 ctttacagcc catgtcaaac aaggtgacaa ggttaaagca ggtgacaaat tgattagctt
     6721 tgacatcgat gtcatcaagg ctgctggtct cgtcgcggaa acaccagtta tcgtgaccaa
     6781 ccaaactgac tttgacactc aagttatcgg aaatcttcct cgtgccatct cgcaaggcga
     6841 agctattctg acagttacaa agaattaatg ttctaggagg gcgcaaggta acttgcacct
     6901 cctatcattg cttatgcaag aaggaaaaat caagaaagag agagcaaaat gacaatagac
     6961 aaacgaaagg ttgtttatca aatctaccca aaatcttaca aggatacaac tggaaatggt
     7021 gtaggggatt tacgaggaat tattgaaaaa ctcccatatt taaaagagct aggtattgat
     7081 atgatttggc tcaacccttt ctacccaagt cctcaacgag acaacggtta tgacatttca
     7141 gattatacag ccgtcaatcc tgattttggc accatggctg actttgaaga aatggtgact
     7201 gtcggcaaag aactaggcat cgaattcatg ctagacatgg tcctcaatca ctgctcaacc
     7261 gaccacgaat ggttccaaaa agcccttgcc ggcgacaaat attaccaaga tttctttatc
     7321 ctgcgtgacc agccaactga ctgggtgtcc aagtttggtg gcaatgcctg ggctcctttt
     7381 ggagatactg ggaaatacta cctccaccta tttgatgtga cccaagctga cctcaactgg
     7441 cgaaaccctc atgtccgtga agaactcttc aaggtggtca acttctggaa agacaagggt
     7501 gtcaaaggct tccgcttcga tgtcattaac ctgattggta aggacgaagt cctcgaagac
     7561 tgcccaatca atgatggcaa accagcttat accgaccgcc caattaccca cgactacctc
     7621 aagatgatga acaatgccac ttttggggca gaaaaaggct tcatgaccgt cggggaaatg
     7681 tctgccacca ccattgaaaa ctgcattctc tacacagctc ctgagcggga agaattgtcc
     7741 atggccttca acttccacca cttgaaagta gactacaagg acggtcaaaa atggaccatt
     7801 atggacttcg actttgaaga actcaaacaa ctcttccaca cttggggcga agagatgtcc
     7861 gccggaaatg gctggaacgc ccttttctac aacaaccacg accaacctcg tgccctcaac
     7921 cgctttgttg atgtggagaa tttccgcaag gaaggagcaa ccatgctggc agcctctatc
     7981 cacctgtcac gcggtactcc ttacgtctac atgggcgaag aaatcggcat gattgacccc
     8041 gattacgaca gtatggacga ctatgtggac gtggaatcta tcaatgccta tcagatgctc
     8101 ttggaccaag gtcacgcacc tgagcaggcc ttcaagatta tccaggccaa gtcccgcgac
     8161 aattcccgca ctccgatgca atgggacgct tctgacaatg caggcttctc aacaggcacc
     8221 ccttggctaa aggctggcaa atcctatcca accattaacg ttgaagccga aaaaacaggc
     8281 ccaatcttta ccttctatca agaactcatc cgcctgcgga aagaactccc tctgatttca
     8341 gaaggggact acaaggcagc ctacaaggat agccagaaag tctacgcctt tgaacgcttg
     8401 ctaaatggcg aaaaactgtt ggtcctcaac aatttctttg cagaagaggt tgaactagat
     8461 ttggcagacg actatgccca cggccaagtc ctcatcagca actatcctga caacaaactc
     8521 ggtaagaaaa ttaccctcaa gccttatcaa gcactggcaa ttcaagttaa ataaacgata
     8581 aaacatccga aaataacact ctacgtgtca attttaattt tcctaaaatt agaacccgtc
     8641 ttcacagctc agtaggttac ttgtgaaggc aagttcttgg tttaaggttt cttttaggaa
     8701 tatattttat acttagtttg cgcacgaaac agctatctgg ggggagatag ttgtttcttt
     8761 ttttatagtc atttgaatta actttgatac atcgtcactt tcggcttgcc atactctacg
     8821 aaagtgactt gtttcgcaag tcttattttc aacctagaac agcctctagg ctgttctagc
     8881 tacctgcacc tcagttcctt gtctgaaagt tcattcattc gactatatta ctacatataa
     8941 agcaataacc gtttcagata aggctgaaac ggttattttt gtaagtctaa ttaaaagata
     9001 catgcacatg atcgtagtgg ttttctgtaa cgctaccacg gtctggcatc agattccatg
     9061 tataggctgg accatagata ctgtcatacg gcgcatagaa gcgctgtttc caaatgatat
     9121 agttgatatt tttagaagct aagtttgcaa ctgcataagc cgccacttga tctcctagag
     9181 ctgagctctc aggcaccata aagtcaattg ccaatccctt accatggtcg ccagaatcac
     9241 caggacggta acctgagaaa gaagtaatac cgaaggcatt tgccacttct gcgcggaagg
     9301 ctgctacctg aggttgcaaa ccgacatcat aagcactagt acttgttgta gtggttgata
     9361 gggctgtcac ttgtggagtt tcaactacag gctgttccac aggtgcctca accacaggtt
     9421 gctctacaac ttcctctaca ggcgcttcta ctacaggctg ctctacaact tcctccacag
     9481 gtgcttctac tacaggttgc tctacaactt cctctacaga cgcttctgct acaggctgtt
     9541 ctacaacttc ctctacgggt gcttctacta caggctgttc cacaacttcc tctacagacg
     9601 cttctgctac aggttgctcc acaacttcct ctacgggcgc tgagtcggta actgatgaaa
     9661 tttgatccaa gggaacaacc gtatcctcaa ccgttacttg attggttgtt aggtcaacaa
     9721 ttgcctgaac ggtctcattt gtgtttccct gaacaggagc ttcaatctca acctgagtca
     9781 cttgattgtt ttggtcaaca gtcgtcgtca gtaccgtatc agggaaaatc aagttgacat
     9841 ctgcaatttg atttattttc gccagcaagt ccatatcaat attcatagct aaggcgatgg
     9901 cagataaggt atctccatac tgaacagtat aggtcatctt tccttcttct tgtacaattt
     9961 ctgggctaat ttcagaaaca gtacgtggag cccattcgat ggctaaaaca tttgtcgaaa
    10021 tcatccctgt actaaagagg gaaagagcca atgaagatgt gtataacatc tttttattgt
    10081 ttttcatgat gggtgttttt ctcttgcgac gcatatgttg tggtttattt gttctcttac
    10141 gtttcataat cttattcctt ctttattttt gagatggttc tatcttacct aaattaaatc
    10201 tgaaaaacaa gccttcaacc gttcttttaa ataaactgtc tccactatgt aatcttagga
    10261 cttttctgtc acacaatcaa tataaatgtc atctgactgt catattatta gataatactc
    10321 aatgaaaatc aaaaacagac tcggagacac agatgcagat agaacaaaag ttcatcaaat
    10381 caagtcaaca acgtctggat tttcgaagac tataagtaaa aaataagagt aaggcagtaa
    10441 tccaacaatt ggctactaac tcactctcat ttctattttc ggattagtca aaaacctaca
    10501 tttcttcagg ataaaccaag ccccattctt tacggagttg ggtcatgatc tccatgacat
    10561 cttgggtgta ggctaggtgc atggtgtttt cgatgcctgc gacagctttt tccatatccg
    10621 ccacttcata agaaagcgcc ttggctgtat cgcccgcttc gattacctct tggctaccgt
    10681 cttcagtata ggtaatgact gccttctgac cacgaggata ctcgtaaagt tcgatgtagc
    10741 ccttgtcgta ggcaatagta ccccgttttg gctgtttggc atggagggtc agggcgatgg
    10801 tcgccatttc ccccgcatca ttgctgagca aaatccctac ctgctcgtcc acacctgtcg
    10861 gagctaattt aacctgagat aggacttgat ttggcttttc agtcataaac caacggacaa
    10921 atgacagagc gtagacacca atatccaaga gggcaccgcc tgccaagttt ttattgaaaa
    10981 agcggttggt catgtcatat tccttgtagc tgccaaagtt catctgaatc atcttcaaat
    11041 ctccaagttt tccactggca acaacttcgc tcaactgacg atagattggc atgtggaaaa
    11101 tggtcatagc ttctaccagg ataacctgat tttcttctgc cagttgaatg gcttctgcta
    11161 attcttctga attaagcgta atagactttt cacaaaggac atgctttcca gccttcaagg
    11221 cctttctcaa ataattgata tgggtattgt gaggcgtaga aatatagata atgtctactt
    11281 caggatcctc aaacacttca tcgatttctc gataaacttt ctcgatgcca tatttttgcg
    11341 caaattccac acccttgtca taggtacggt tggctaccga ataaagattt cctaccatag
    11401 cctgcaaggc ctgaaccaat tcattggcaa tgacacctgt tcccagggtc gcccatttat
    11461 acttcatttg cacttccata gtgtacctca ctttcttcta ccattatagt aaattccacc
    11521 cttttctgca cactctaact agcatttgtg ttacaataag aacatgaata ccgttgtact
    11581 aaaggcatct gcccagcaaa aacaagccat gatggctcac tatgaccgct atcggcagac
    11641 cagcaaaaat ccttacatag aagcattttt caaactgacc ggtgccagcc tgtccatcta
    11701 cacttctgga aaagtcgttt ttcaggggga aatggcagag caggaagcag ggctctgggg
    11761 ctatgagcca gaaagctctg gacagacgac tgtacctggt caaaacctgc ccatgattgg
    11821 taccgatgag gttggaaacg ggtcttattt tggaggtttg gcagtcgttg ctagttttgt
    11881 ccgcccagaa gaccatgcct ttctcaagtc gcttggtgtg gatgattcca agaaaatgac
    11941 agaccaaaaa atctgccaag tcgcccctct cttaaaagag aaaatccccc accaagctct
    12001 gctattgtca ccgaaaaaat acaacgaagt catcgagcaa ggctacaatg cggtgtctgt
    12061 caaggtcgcc ctgcacaatc aggctatttt cctactcttg caaaagggtg tccagcctga
    12121 gaagattgtc attgacgctt ttacttccag tcaaaattat caaaagtact tgaagaagga
    12181 agccaaccaa tttgccaacc ccgtcactct ggaagaaaag gctgagggca agtacctggc
    12241 tgtggcagta tcttctatta ttgctagagc tatgttttta gaaaatcttg tccagcttgg
    12301 tcaactagtt gggatgaatc tgccgtctgg tgcgggcagt aaatccgacc aagtcgcagc
    12361 aagcatctta aaacaatatg gtatggctgg tctcaaccaa actgccaaac tccactttgc
    12421 caatacacaa aaagcacaaa aattattgaa atgaggaaaa aatatgggag caatccaaaa
    12481 aacaaaacgc tcaccactag tcgccttctt agctgaatgg ggtatctttt tactatttat
    12541 ggcagctttt tttgccagtc gttactttat ctggaaccct gtatctgtcg atggccattc
    12601 tatggaccca acgcttcaac accaagaaaa actcatcatg ctaaaaacaa cctctatcga
    12661 ccgctttgat attgttgtag ccagtgagac agacagcgac ggcaaggaaa aactgattgt
    12721 caaacgtgtg attggtatgc caggtgacac cattcgctac gaaaacgatg tcctctatgt
    12781 taacgaccaa aaagtggacg agccttactt agatgaatat ctagctgcat tccagaaaga
    12841 taaacttcaa gaagtctatt cttacaacaa acaattccaa gctgtggccc aatctgctga
    12901 agctttcaca caagatgcca atggttacgt agactttacc gtgacagtcc cagaaggaca
    12961 atactacctg atgggagatg accgccttgt ctcactagat agtcgtagtg taggaacatt
    13021 ttcccgtgaa aacatcaaag gagaagttgt ctttcgcatg tggcctctca accgtattgg
    13081 gactgttgat taatacaaac aagtttggag caatccgttc caaacttctt tttcattatt
    13141 attggaacta aactctatga acgaagtgta ttttaccggt actattgacc ggattatttt
    13201 tgaaaatcct agtaattttt ataaaatcct tctccttgaa atcgaggaaa ctgacgctga
    13261 ctatgacgac tacgaaatta ttgtgaccgg aacgattgcc gatgtcattg aaggagaaga
    13321 ctatcgtttt tatggcaatc tggtcaccca tcccaagtat ggtcagcagc tgcaaatttc
    13381 gcgttacgaa cgcagcaagc ctacctcagc tggtctggtt aagtatttct ccagcgagca
    13441 tttcaaggga attggacgca aaacagctga aaaaatcgtt gagctctatg gcgaagatac
    13501 cattgataag attttagctg aacctgaaaa actaacccaa atcacaggcc tatccagcaa
    13561 gaacatgcag gcatttgtcg aaaaactccg cctcaattat ggaaccgagt tgatcttggc
    13621 aaaactagcc gagtatggca ttcccaataa attggccttt caaatccaag accagtataa
    13681 ggaaaaaaca cttcagatta ttgaagaaaa tccctatcaa ctggtcgaag atgtccaggg
    13741 gctcggcttt accattgccg ataggattgc ggaaaacctg ggaattgcca gtgattcacc
    13801 ccaacgtttt cgggctggca tgctctttag tctcattcat cgctccatgg aaacaggcga
    13861 tacctatgtg gaggccagag acttgctgga agcaactctt gaactgctgg aaaaatcccg
    13921 ccatacagag ctggaccccg ctgctgttgc caaagaattg acgggactga ttgcagacga
    13981 caaggtacag caagaaggca caaagatttt tgacaacagc ctctactttg ccgaacatgg
    14041 tatccataaa aacttgaccc gcctgatggg aaaaaatggt ttcaaaccct tcccacgcac
    14101 agacgttgag gctgctatag cagagctgga aactatgtct tccctgacct acgatgacat
    14161 tcaaaaagaa gccatcgtcc aagccattac caatccgctt ttcattctga caggcggacc
    14221 agggactgga aagacaaccg ttatcaacgg tattatcgcg gtctatgcca tcttgcataa
    14281 gattgacctg actcgcaacc gagaagaatg cccagtcctc ctagccgctc caactggacg
    14341 agcagccaga cggatgaatg aattgacagg tttgccttcc gccaccatcc accgccatct
    14401 cggtctggta gaaggacagg aagaatccta ccgtgatgat tatttggatg ccgactttat
    14461 catcgtggat gagttttcta tggtagatac ttggttggca aaccagctct tccagaatat
    14521 ctcctcccag acccaagttc tgattgtcgg cgatgccgag caattgcctt ctgtcagccc
    14581 cggtcaagta cttgctgacc tcttgaaaat tgacaagctg cccagcatta ctctagaacg
    14641 tatctatcgt caatccgatg attcgaccat tgttacccta gccagccaga ttcgacaggg
    14701 tgctctgcct agcgattttc gtgagaaaaa ggctgaccgt tcctattttg aagcccagaa
    14761 tgaacaaatt ccagccctga ttgagcgcat tgtcggcgca gccatcaagt caggtattcc
    14821 tgcaaacgag gtacagatcc tcgctcccat gtaccgtggg gccgcaggca tcgaccaact
    14881 caacactatg acccaggccc tgctcaatcc actggaagag ggagagttag aattcctgca
    14941 taacgagcaa gccttccgcc aaggcgaccg ggtcattcat ttagtcaacg atgccgaggc
    15001 caatgtcttc aatggtgatt taggctacat caccgacctc cttcctgcca agtacaccga
    15061 ctccaagcag gacgaaatta ccatcaactt cgacggtagc gaggtcacct atccccgaaa
    15121 cgaatggtac aagattaccc tggcctatgc catgtccatc cacaagtccc aaggcagcga
    15181 gtttcaggta gtcatcctcc ccatcacccg caccagccac cgtatgctcc agcgcaatct
    15241 ggtctacacc gccatcaccc gctccaagag caagctcatc ctcctgggcg aaatctccgc
    15301 cttcgactac gccgtcaaaa acgccggcac cgtccgaaaa acctatctgg tcccacgttt
    15361 ccaaggggaa atggcagagc aggaaagcaa ggaaagcctg attaaaaaag acgaaagcaa
    15421 aacagctaca accacgcaac cccagccccc tactcagcca gacagaaaag aacaggttaa
    15481 agtagtcaaa gaaaacaacc aacaactctc ccttcttgat caagaccagc caaaaaaaac
    15541 aagtccccaa cccacagaat acattttgac tgtagacaac ctactaacca tcgaccccat
    15601 gattggcata gaacaggcag atattgaaga attttttaaa gcataaaaac cagcaagtca
    15661 gtagatgaac accgttatat tcttttattt gacagcctct aaagctgctt tataatctgg
    15721 gtgttcgttg acattgtcta ggtattctac gtagacgatc tcattgtcgg cattaaggac
    15781 aaagaccgca cgcgccagca aattccactc ccgcatgagc acaccatagg attttccaaa
    15841 ggcatggtca tagtccccta ccatggcgac tgtcttgccc tgttcttgta agaacttgac
    15901 ttggttggcc ttgtcatctg gcaggacctg actgaccacc tgctcaatgc cgacttcctt
    15961 ggcaatagcc tgggcagttt tttcattatc tcctgtcagc atgacaacct ctagttccat
    16021 ggtttgcaga gcctggacag cctgacggct ggtctctttt actttatcag caatggcaat
    16081 gactgctagt acttcttgct ggctggctag gaaaacaggc gtcttagctt gatgggcaaa
    16141 tgtttccgca actgcacgtc ctttggaaac atcaattccc tgttcccgca tcaaccgttc
    16201 atttcccaga tagatggtct gctctgctat tgttaccgaa agaccacgac cagaaagtgc
    16261 ctgaaaatct tggctggcaa gtaagggaat tgattcctct tctgctgaac gtaagagggc
    16321 ttgagccaat ggatgctcgg agtattgttc actgcttgct gccagttgga gaacttgctc
    16381 acgattttta gtagacaaaa ggtggatgtc tgtcacttgt ggcttgccct cagtaatggt
    16441 cccagtcttg tcgaagacaa tggtgttcac accttgtagg gttttaatag tttgaccaga
    16501 cttgaacaaa agaccatttt ctgccccttt tccagtccca accatgatag cggttggtgt
    16561 ggcaaggccc aaggcacacg ggcaggcaat gacaaggacg gagatggtaa tggaaagtga
    16621 gaacatctag ggctcttgtc ccaagaagta ccaagcaagg ccagaaagga cggctaatcc
    16681 cattacaatt ggaacaaaga ctgctgaaac gcggtcagcc aactgcgcaa tcggagcttt
    16741 tgacccctgt gcttcttcca ccaaacgaat aatttgggac aatgtggtat ctgctccaac
    16801 ttttgtcgct tccatttgga aagcaccatg ttgattgaga gtcgcaccaa cgactgtatc
    16861 cccgatgttt ttttaacagg caaactttcc cctgtcagca tggattcatc aattgtcgat
    16921 tggccttcta ctattatccc atcaactgga atctgttcac cagggcggac ttgaaggaca
    16981 tctcctactt tgacttggtc taaggggagg gtcacttctt gaccttcaca gagaacgcgt
    17041 gcagttttag gggcgagatt catcagttta ctgatagctt ctgaagtacg ctcttttgat
    17101 aatgcctcca tgtattttcc aagagtgata agggttaaaa taacagctgc cgattcaaaa
    17161 tagagctcag gatgtccatg ttgggtggct acttggttgg tagcaatcaa aatcgtcagt
    17221 cataatgatc ccttgaaaca aggcagcact tgtcccaacg gcaatcaaac tatccatatt
    17281 gggatgaccg gcaaacaggg ttttaaaccc tttttgataa aatgcacgac caacatatag
    17341 gactggtaat acaagaatga gctgaactag ggcaaaaact agcggctggt gtaagatagc
    17401 tggcaatggc aatcccccac ctggtaacat aggtcccatt gcaatgtaga gtagtggaag
    17461 cgtgaaaatg gttgaccaga taaatctgtg ccataaatca tttttgtgtt gttcttttcc
    17521 gcttggttga gcttctgact cttgtgtttt tggcagtacc gctttataac ctgctttttc
    17581 aacagctgtc gatatctgat aagctcagat gggctggatc atactgaatg tttgtttttt
    17641 ccgttgctag attcacactg gcgttttgta ttccatctag cttattcaag gctttctcga
    17701 ctgtcatagc acaggaagcg caggtcacgc cttgaatggg atagcttgct tgtttcatag
    17761 attactcctt ttctgactac atttgtaaac aaattataca acatccgact acatttgtaa
    17821 acaattttga ttaaaaaaat gagagaatct tctctcatta ggtatctgat caaaagggta
    17881 ataaacttat atatattctg tatgtgtttg aacataggtt aatttgctaa cgattcattt
    17941 agatagatag aacaatttgt atggatttgc attagagttg atggaaatcc agtatctggc
    18001 ttagaacaaa ctgttttacg tatgaccgca tgatgccaat cacgaaaaac tgcaagtcga
    18061 atttttttcg cttgaaaaat ctcgctcatt cctattgtta cacaataatt aggcacttct
    18121 tctacagctc cttttaatac cgtcaaacca ttcataacct ttgtctcgac agaaataggt
    18181 aacactcgcg accctaattt taaaaattct tgaactgtta atgttgaatc cggttcattg
    18241 aatgcaatgt gcccatttag gccaattcca ccaatacata agtcgatttt acctaattct
    18301 ttgattttat ttgaaatcga gtctatatca agaggatctg gaaaaattct ttgttcttct
    18361 gacataataa gagttggatg tatttgactg tagacatttt tctgcataaa tcctcgaaaa
    18421 ctaagagggt gtactacgtc tatccaatgc aaatcttcca acaaatattc atccatgtta
    18481 ataaaccaaa cattagacaa atcaatctta aattgattca ctaatctaac aaaatgcgga
    18541 tactgaccaa ctggtccaac aggacaaatt atgacagttt tctcattttt ttc
//
LOCUS       DBNFEG010000022        17435 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.022, whole genome shotgun sequence.
ACCESSION   DBNFEG010000022 DBNFEG010000000
VERSION     DBNFEG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 17435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17435
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.022"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(104..1015)
                     /locus_tag="AC1PNG_000528"
     CDS             complement(104..1015)
                     /locus_tag="AC1PNG_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIP6701058.1"
                     /translation="MRIQQLYYIIKIAETGSMNEAAKQLFITQPSLSNAVRDLEKEMN
                     IKIFLRNSKGITLTKDGMEFLSYARQIVEQTELLEVRYKNPNAKRQLFSVSSQHYAFV
                     VNAFVSLLKETEMEDYELFLRETRTWEIIDDVKNFRSEIGVLFLNHFNRDVLLKLLDD
                     NRLSYTTLFTAKPHVFVSKTNPLAQRKSITLDDLVDFPYLSYEQGIHNSFYFAEEILA
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLEFDDEIELVYI
                     QHEKALLSDMGEKFINYLLEEVKFDQK"
     gene            1230..2003
                     /locus_tag="AC1PNG_000529"
     CDS             1230..2003
                     /locus_tag="AC1PNG_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HIP6701059.1"
                     /translation="MILKEQMLLVDQVQLAPRIFAMTLQGDMVQDMKMGQFIHIRVPD
                     DSMLLRRPISISEIDRNKSQCRIIYRVEGQGTEVFSKMTVGQYLDVMGPLGKGFEVDF
                     LKEGDRILIIGGGIGVPPLVEVAKRAAFRGVNVTSVIGFATKEAVILEEELASYGMVY
                     VTTDDGSYGRKGNVATVVEELTNEFAAIYSCGAPAMMNYVDQRFQEHPHAYISLEARM
                     ACGMGACYACVVKPKEGQEHENKRVCKEGPVFATGSLIL"
     gene            2013..2954
                     /locus_tag="AC1PNG_000530"
     CDS             2013..2954
                     /locus_tag="AC1PNG_000530"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIP6701060.1"
                     /translation="MIKRLAISLPGLDLKNPIIPASGCFGFGQEYADYYDLNQLGSIM
                     IKATTRHPRYGNATPRVAETPAGMLNAIGLQNPGVDVVLSEKLPWLEQHFPDLPIIAN
                     VAGFSNEEYAYVSGKISKAPNVKAIELNISCPNVDHGNNGLLIGQVPELAYQAVKAAV
                     EASSVPVYVKLTPSVADITLLAKAAEDAGATGLTMINTLVGMRFNLKTRQPILANGTG
                     GMSGPAVFPVALKLIRQVAQVTDLPIIGMGGVDTADKAIEMMVAGASAVGVGTANFTD
                     PFACPKIIQDLPKRMEVYGIDSLEGLRREIRHRLGKR"
     gene            3119..3814
                     /gene="pyrF"
                     /locus_tag="AC1PNG_000531"
     CDS             3119..3814
                     /gene="pyrF"
                     /locus_tag="AC1PNG_000531"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIP6701061.1"
                     /translation="MKETRPIIALDFAGKEEVQSFLEQFPPDEKLYVKVGMELYYAEG
                     PGIINYLKGCGHSIFLDLKLHDIPNTVESAMKVLAKLGVDMTNVHAAGGVEMMRAARR
                     GLGKDAVLIAVTQLTSTSEEQMRTDQNIQTSLQEAVVHYAQRAQEAGLDGVVCSAHEV
                     ALIKDATSSDFVCLTPGIRPTGGEVGDQKRVMTPADAARIGSDYIVVGRPITKSEHPY
                     QTYLSIKEEWNRG"
     gene            3807..4133
                     /locus_tag="AC1PNG_000532"
     CDS             3807..4133
                     /locus_tag="AC1PNG_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HIP6701062.1"
                     /translation="MDKIYQVIIDRPIGYIDKFGNQYPINYGYVPNLLAGDGEEQDVY
                     IISKAVRQPLTAFEGKLVAIIHRRDDVEDKWVLTGLDEKLTVTEIKEKTNFIEQYFDS
                     WIEMVD"
     gene            4270..4902
                     /gene="pyrE"
                     /locus_tag="AC1PNG_000533"
     CDS             4270..4902
                     /gene="pyrE"
                     /locus_tag="AC1PNG_000533"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIP6701063.1"
                     /translation="MTLATEIASHLLDIKAVHLRPEKPFTWASGIKSPIYTDNRITLS
                     YPETRNLIEDGFVQRIEEEFPEVEVIAGTATAGIPHGAIIADRMNLPFAYIRSKPKDH
                     GAGNQLEGRIVKGQKMVVVEDLISTGGSVLDAVAAAEREGADVIGVVAIFTYELPKAD
                     CNFDNAGVKLVTLSNYSELIKVAKVQGYINADGLNLLKKFRQNQETWQED"
     gene            5011..5937
                     /locus_tag="AC1PNG_000534"
     CDS             5011..5937
                     /locus_tag="AC1PNG_000534"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIP6701064.1"
                     /translation="MISVIVPCFNEEEAIPYFYDAMEKVRKEMGEQFEYIFVNDGSKD
                     RTLTVLRQLSGQDRAVRYLSFSRNFGKEAALYAGLQAAQGELVTVMDVDLQDPPEMLM
                     EMKAMLDGNPDLDCVGTRRVSRDGEPPIRSFFAKLFYKLMNKISQVEVVDGARDFRLM
                     RRHMVDAILSVSEYNRFSKVIFAWVGFETEYLPYENVERVAGETSWSFWKLLSYSIEG
                     IINFSDTPLNIASYTGFFTFLLSLVLMVFVVFKTLVFGDPTIGWPSTICIILFLGGLQ
                     LMTIGILGKYLAKVFLETKKRPIYIVKEKSSN"
     gene            5947..6600
                     /locus_tag="AC1PNG_000535"
     CDS             5947..6600
                     /locus_tag="AC1PNG_000535"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIP6701065.1"
                     /translation="MEHSAWHELIKAQLPEHYFGKINQFLDYVYAGGTVYPPREKVFA
                     AIQTTDLEEVKVVILGQDPYHGPGQAQGLSFSVPNSVPAPPSLQNILKELTDDIGVKQ
                     DHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIKVVNSLDQPVVYILWG
                     AYARKKKALITNPKHLVIESAHPSPLSAYRGFFGGKPFSQANAYLTSQGRTGIDWLR"
     gene            6640..7911
                     /locus_tag="AC1PNG_000536"
     CDS             6640..7911
                     /locus_tag="AC1PNG_000536"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIP6701066.1"
                     /translation="MLLVKNGRVLDPQSGLDKVCDILMDGSEIVKIAESISEDVEQEV
                     IDATGLVVAPGLVDVHVHFREPGQTHKEDIHTGALSAAAGGFTSVVMMANTNPTISNV
                     ETLKEVLDSAKKEAIHIYSVATITDHFDGQNLTDFPALLEAGAVAFSDDGIPLTNAGV
                     VRKAMKLAKEQGCLISLHEEDPDLNGVLGLNEQVAEKHFHVCGATGVAEYSMVARDVM
                     IAYETGARVHIQHLSKAESVKVVEFVQGLGANVTAEVAPQHFSRTEDLLLIKGANAKM
                     NPPLRLESDRLAVIDGLKSGVISVIATDHAPHHADEKNVEDLTQAPSGMTGLETSLSL
                     GLTHLVEDGHLTLLQLLEKMTINPAKLYGLDAGYLAENGPADLVIFDPNANRLVTADF
                     ASKSANSPFVGDSLKGQVLYTIANGQIVFGK"
     gene            8042..10156
                     /gene="nt5e"
                     /locus_tag="AC1PNG_000537"
     CDS             8042..10156
                     /gene="nt5e"
                     /locus_tag="AC1PNG_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface ecto-5'-nucleotidase Nt5e"
                     /protein_id="HIP6701067.1"
                     /translation="MKKKKILLPVMSTLLLAPFVLAQQVQAAETTTAATTTNQPATTD
                     ATATVPATSVENVATEETVVPAAEETVEAVIIHTNDVHGRILEEKNVIGDAKAAAVIE
                     EERAKVENTIVVDAGDAFQGLPISNSTKGEDRANIMNQVGYDAMAVGNHEFDFGMDQA
                     IKYKETLNFPLLSANTYVNGARVFEASTIVDKTPTVVGDEFVVIGVTTPETATKTHPK
                     NVEGVTFTDPVTEVNKVIDEVEARALADNRVYKNYIILAHLGVDSTTPVEWRGSTLAE
                     ALSKNSKLAGKRVIVIDGHSHTVEATTYGDNVTYNQTGSYLNNIGKVTLKSDKLLGEA
                     SLISAADTKNVTPNAKIAALVDEIKAKYEAENAQVVIENNPVELNGDRSNVRVRETNL
                     GNAVTDAIYAYGQTGFSNKTSLAVTNGGGLRATIAKDQPVTKGDIIAVLPFGNIVSQI
                     TVTGQQIYDMFTKSLSSTLQVNPETGEMLLDENGMPLFEASGGFLHISGANVFYDPTL
                     PVEERVLLIGILNPETGEYDALDLEKTYYLATNDFLAAGGDGYTMLGGAREEGPSMDS
                     VFAEYLKTADLSAYEVVNPYSRIIPVNSSIDTDEDGYPDFIEILLDTDPENPASNPET
                     VPAENTDSPSNQVQNTSATDKKAPVDSPKVGDKKTEVASPVKTTKAGVLPNTGDQMNL
                     TLSLFGLGLAGLAMAVGRRKEN"
     gene            10208..10855
                     /locus_tag="AC1PNG_000538"
     CDS             10208..10855
                     /locus_tag="AC1PNG_000538"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIP6701068.1"
                     /translation="MQTVFQYQKEGDREQISSYVLLSNSPRRKQLLGFLQPQIASVEV
                     DERGIEEHFMKQFATDDFLTRAAKTCCEISKAKSDMDLVTGHLYISADTIVVSDGQIF
                     NKPQDLTEAEIMLRSYFGKSHHVVTSVCLRTRHSLDVFYTVAQIDFVDYYPALEEVIQ
                     SYIREKQPLDKAGAYGIQELDPRFVSGISGDIHTIIGLPVAEVSRRIFSEEGFEK"
     gene            10907..11395
                     /locus_tag="AC1PNG_000539"
     CDS             10907..11395
                     /locus_tag="AC1PNG_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HIP6701069.1"
                     /translation="MTVDIERYKQLAQQKQGEHRKFLANLKKKAPKNLDRIVQDIHAE
                     VFSEIDCTECVNCCKTLGPLFTEADITRIAKHLRMKLPVFEDMYLRVDEDGDKVFQSM
                     PCPFLGGDNLCSIYDARPKACREFPHTDRKKIYQINNLTIKNTLICPAAYLFVEKLRE
                     RI"
     gene            11495..12346
                     /gene="ylqF"
                     /locus_tag="AC1PNG_000540"
     CDS             11495..12346
                     /gene="ylqF"
                     /locus_tag="AC1PNG_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002962311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HIP6701070.1"
                     /translation="MATIQWFPGHMSKARRQVQENIKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKVDLADPVRTKEWQSYFEGQGIRTLAINSKEQATVKKVTDAAK
                     SLMKDKIERLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKDLEILDTPGILWPKFEDQEVGLKLALTGAIKDQLLPMDEVTIFGLDYFKANY
                     PERLQERFKGMDLEQETPELIMDWTKKLGFRDDYDRFYNLFVKDVRDGRLGTYTLDRV
                     SDLDGND"
     gene            12333..13112
                     /locus_tag="AC1PNG_000541"
     CDS             12333..13112
                     /locus_tag="AC1PNG_000541"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIP6701071.1"
                     /translation="MATIKEVTTLLAQVDSLDSPVWAELSQDERAGVQAAIKKRRKEL
                     DKEVAEDARLEAMLFYEKNLYENGVEFIAGIDEVGRGPLAGPVVAAAVILPKGCKIRY
                     LNDSKKIPKSKHEAIYQEVMERAVAVGVGIKDAAVIDQVNIYEATKLAMLEAFGKLSQ
                     KPDHLLIDAMKLDTPIPQTSIIKGDANSLSIAAASIVAKVTRDKMMADYDKEFSGYGF
                     AKNAGYGTAEHLEGLNKLGITPIHRKTFEPIKSMVTGGKSS"
     gene            13109..14119
                     /locus_tag="AC1PNG_000542"
     CDS             13109..14119
                     /locus_tag="AC1PNG_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIP6701072.1"
                     /translation="MTVFFWIFLASIGLFGLLYFWERRSIFISLFALNILVWALAWII
                     STGILENNEILRLLAIAVAMVLFLAFLSGPFVLLFTLYLNGFQILEREGVRFHNFLSM
                     GLAVALTFYLFIAPFVVQSLSGISLLNMVFVYVGFLVSYAIIISMLYTTSSFVNFVNL
                     FPGKLDYVVVLGAGLIGDKVTPLLASRIDKGIAIYQKQPGSKLIMSGGQGPDELIAEG
                     QAMANYALEQGIPAEDIIIENQSTNTEENLKFSYALMKPGSRFALVTNYYHVFRALLL
                     ARQLKIKCIGYGAKTKFYFSLNAFIREFVGYVVMSRKTHLVFLSIVSALYLIGVIISL
                     MH"
     gene            complement(14152..14802)
                     /gene="trhA"
                     /locus_tag="AC1PNG_000543"
     CDS             complement(14152..14802)
                     /gene="trhA"
                     /locus_tag="AC1PNG_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HIP6701073.1"
                     /translation="MNPSLKLSLQLSFGEEVANSVTHAVGALLMLVLLPVTAIHSYQD
                     YNLTAVIGMSIFVISLFLMFLSSSIYHSMPYASTHKYILRIIDHSMIYIAIAGSYTPV
                     ALSLVGGWLGYLIVGLQWGLTIFGILYKIFAKKINEKFSLFLYLFMGWLVVFILPAIL
                     QKTGMAFGILMLAGGLSYTIGALFYAKKKPYFHMIWHLFILLASALQYIAIVYFML"
     gene            complement(14792..15343)
                     /locus_tag="AC1PNG_000544"
     CDS             complement(14792..15343)
                     /locus_tag="AC1PNG_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HIP6701074.1"
                     /translation="MKQTIPYWHELPNLDLYLDQVLLYVNQVVNSQESLEQKSLYSMK
                     INSSLGNETDDRTGVHQGKLTTDNVDFRRVLTAAMINNYVKHKQIEKPVKKKYQKQQV
                     ARLIALTILKNVFSIQEISQTLHLLLQSSHSETLYNHFVDCMRNEENEETPDIIRYAC
                     QSVKLYYKTRQLTMELERSHHES"
     gene            complement(15451..17103)
                     /locus_tag="AC1PNG_000545"
     CDS             complement(15451..17103)
                     /locus_tag="AC1PNG_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIP6701075.1"
                     /translation="MKIIIVGGVAGGMSAATRLRRLMEDAEIIVFEKGPYVSFANCGL
                     PYYISGEIAEHDSLLLQTPESLKQRFNLDVRPFHEVISIDAEQQTVTVKTPTGELIES
                     YDKLILSPGAKPVIPNLSGIEEANNLFTLRNVPDLDKIMIKLDQIKSGHVVVIGAGFI
                     GLEMAENLALKGWKVTLVEQAPHVLPTFDLEMARYVEAELTNKGIEVITGQSVTAFHE
                     KGQVLELSNGQTLSSDVTILSVGVAPDSQLAKKTGIELGLRGGILVDKNYQTSQNNIY
                     AVGDAIVVKQEITGQDALISLASPANRQGRQVADVIAGLDRKNKGSIGTAIVKVFDLA
                     AASTGLSERTVQSLGYEHAIIHTTGKDHAGYYPGATDIVLKLIFSPTDGKIFGAQAVG
                     QKGIDKRIDVLATAIKAGLTIFDLPELELTYAPPFGSAKDPVNMIGYAAMNITEGISQ
                     NIQWHQLDDELATGKVLLDVRNPGEVANGQFREFIHIPLDQLRERIGELDASKEYIVI
                     CHSGLRSYIAERILKEKGFGVKNLDGAFSLYKMALPENIIQP"
     gene            complement(17100..17423)
                     /locus_tag="AC1PNG_000546"
     CDS             complement(17100..17423)
                     /locus_tag="AC1PNG_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIP6701076.1"
                     /translation="MFKQLFSGMFSNSDSLPITDVEQAIQSSNAILLDVREVDEFASG
                     HIPQAINQPLSQLDQFKGDKTKNYLVICQSGMRSQRATEYLKSQGYQAINIKGGMNAW
                     TGGIA"
BASE COUNT         5043 a         3354 c         4019 g         5019 t
ORIGIN      
        1 gcgtttcctt aaagaaaagt gaattatttt catttttctt ttcaacgtct ttcataaatg
       61 tatcataaca tattgtattt tgtttgtcta tataaaattg tatttatttt tggtcgaatt
      121 ttacctcttc aagtaggtaa ttgatgaatt tttctcccat gtcggacagc aatgcctttt
      181 cgtgttgaat atagaccaat tcgatttcat catcaaattc cagaggaatt gatacaatat
      241 tatctccgtt aaggtttgaa ttgaggatac ctgttgcgat tgtatagcca tctagaccga
      301 ttaagagatt gaagagtgtg gcacggtcag atacgacaat cgatttttta tggtgttcct
      361 gggctagaat ttcttctgca aagtagaagg agttgtgaat accttgctcg tagctgagat
      421 aggggaaatc taccaaatca tcgagagtga ttgactttct ttgtgccaaa ggattggttt
      481 tgctgacaaa tacatgtggt ttggcagtga agagagttgt ataggatagt cgattgtcat
      541 ccaaaagttt cagcagaaca tcacggttga aatggttgag gaaaagcact ccgatttctg
      601 agcggaaatt cttaacgtca tcaatgattt cccaggtccg tgtttcacgt aggaagagtt
      661 cataatcttc catttcagtc tctttcaaga gtgagacaaa ggcgttgacg acaaaggcat
      721 agtgttggga agatacgctg aagagttggc gttttgcatt cggattttta tagcgaactt
      781 ctaaaagctc tgtctgttca acaatttgac gagcatagga aagaaattcc attccatctt
      841 ttgtaagtgt aatacctttg gagtttctaa gaaaaatctt aatattcatt tctttttcca
      901 agtcacgtac tgcattggaa aggctgggtt gggtaatgaa gagttgtttg gcagcctcat
      961 tcatactgcc tgtttctgca attttgataa tatagtataa ttgttggatt ctcatacctc
     1021 tattgtaaca tgatttgaca aattattcta aaaaatgtat aatgctgaaa atatatcctt
     1081 taactgagtc cagagaggcg aagaagggaa cgtgataaat agggcaaata ctgccttctt
     1141 ttcgtgtaac cttgctagcc agcaaggttt ttttctctat atcgactttc ttgttttctg
     1201 tgtcattgtt atatagaata ggagaaaaga tgattttaaa agaacagatg ttactggtgg
     1261 accaagtcca actagcgcca cgaatttttg ctatgacctt gcagggtgat atggtccaag
     1321 atatgaaaat ggggcaattt atccatattc gtgtgccgga tgattccatg ctcttgcgga
     1381 gaccgatttc tatttccgag attgatcgga ataagtctca atgtcggatt atttaccgag
     1441 tggaaggtca agggacggaa gtcttttcga aaatgactgt tgggcagtat ttggatgtca
     1501 tgggaccgct tgggaaaggg tttgaagtag actttctcaa agaaggtgac cgcattctca
     1561 tcattggcgg aggaatcggt gttcctccct tggtggaagt agccaaacgg gctgcgttcc
     1621 gtggtgtcaa tgtcacgtca gtcattggct ttgccaccaa agaggccgtc attttggaag
     1681 aggagctggc aagttatggg atggtctatg tcacaacgga tgatggttcc tatgggcgta
     1741 aggggaatgt cgcaacggtc gttgaagagc tgacaaacga gtttgcagcc atttactcat
     1801 gtggggcgcc agctatgatg aactatgttg atcaacgctt tcaggaacat cctcatgcct
     1861 acatttcact ggaggctagg atggcttgcg ggatgggggc gtgctatgcc tgtgttgtca
     1921 aacccaaaga gggacaagaa cacgagaaca agcgtgtctg taaggaaggt ccagtctttg
     1981 cgactggtag tctgatttta taggaggttg atatgataaa acgattagcg atttccttac
     2041 caggcttgga cttgaaaaat cccatcattc cagcctctgg ctgcttcggt tttggtcagg
     2101 agtatgcaga ttattatgac cttaaccagc ttggatccat tatgattaag gcgactactc
     2161 gtcatcctcg ttatggcaat gcgactcccc gtgtagccga gacaccagca ggtatgctca
     2221 atgccattgg acttcaaaat ccgggtgttg atgtagtctt atccgaaaaa ttaccatggt
     2281 tggaacagca ttttccagac ttgcctatca ttgccaatgt ggctggtttt tccaatgaag
     2341 aatatgccta tgtgtcaggg aaaatttcaa aggctccaaa cgtaaaagct atcgagctga
     2401 acatttcctg tcccaatgtg gaccacggaa ataatggtct cttgattgga caggtgccag
     2461 aattagccta tcaagcagta aaagcagcag tcgaagcctc atctgtacct gtttacgtta
     2521 agctgacacc aagtgtggca gacataaccc tgttggccaa ggctgctgag gatgctggag
     2581 cgactggctt gacgatgata aataccttgg ttggtatgcg attcaatctc aaaactcgac
     2641 aaccaatctt ggcaaatggt acaggaggaa tgtcaggtcc agcagttttt ccggttgctc
     2701 tcaaactcat tcgtcaggtt gctcaggtga ctgatttacc gattattggt atgggtggtg
     2761 tggatacggc ggacaaggct atagagatga tggttgcagg agcttcagct gtaggggtag
     2821 gaacagccaa ctttacagac ccatttgctt gtccaaagat tattcaagac ttgccaaaac
     2881 gcatggaagt ttatggtatt gatagtttgg aaggtttacg ccgagaaatc agacatagat
     2941 tgggtaagcg ttgacaaagg taaaataaaa gagtaatata aagaaaatat ctcctttaac
     3001 taagtccaga gaggctagga aggtgtcatg taatatgctt gaaaggtgat tttcaagctg
     3061 aattcgtgca catccctgac cgatttggca gggatttttg tgtaaatgaa aggattttat
     3121 gaaagaaaca agaccaatta ttgcccttga ctttgcaggt aaagaagaag ttcagtcttt
     3181 tctggaacag ttcccaccag atgagaaact ctatgtaaag gttggaatgg agttgtatta
     3241 tgcggaggga ccaggtatta tcaattattt gaaaggctgc ggtcacagta tttttctaga
     3301 tttgaaactg cacgatattc ctaataccgt cgagtctgct atgaaagtct tggcaaaact
     3361 cggtgtggat atgaccaacg ttcacgctgc tggtggtgtt gagatgatgc gggcagcacg
     3421 aaggggattg ggtaaggatg ctgttttaat agcggtcact cagttgacct ctacctcaga
     3481 ggagcagatg agaacagacc agaatataca gactagtttg caggaagcag ttgttcacta
     3541 tgctcaaaga gcacaagaag ctggtcttga tggggtagtt tgttcagcgc atgaggtggc
     3601 tttgattaaa gatgctacaa gttctgattt tgtgtgcctg acgccaggga ttcgtccgac
     3661 tggtggtgaa gtaggtgatc agaaacgagt tatgacacca gcagatgctg ctcgaatcgg
     3721 tagtgattac attgtggtag gtcgcccgat tacaaaatca gaacatcctt accagaccta
     3781 tttgtctatt aaagaggagt ggaatcgtgg ataagattta tcaggttata atcgatagac
     3841 ctatcgggta tattgataaa tttggcaacc agtaccccat caattacggc tatgttccaa
     3901 atctattggc tggagatggt gaagagcaag atgtttacat catttccaaa gctgttcggc
     3961 agcccttgac agctttcgag gggaaattgg tcgcgattat acatcgcaga gatgatgttg
     4021 aggataagtg ggttctgaca ggccttgatg aaaaacttac cgtgacagaa ataaaagaga
     4081 aaaccaattt cattgaacag tattttgatt catggattga aatggtggac taagagaagt
     4141 ctgtaagaat tcaacctgga taaatatagt ttttataggg ttcatagatt gaaccaatgc
     4201 tttaggagtg acaggctatt cggcgggatg gtcatgttgg aatggtacgc atttagaagg
     4261 aggtctatta tgacattagc aacagaaatc gcatcacact tattggatat taaggcagtt
     4321 catctgcgtc cagaaaagcc atttacatgg gcatcaggga tcaaatcacc tatctataca
     4381 gataatcgta ttacactatc ttatcctgaa actcgaaatt tgattgaaga tggctttgtt
     4441 cagcgtatcg aagaagaatt tcctgaggtt gaggtgattg ccggaactgc cacagcaggt
     4501 attcctcatg gtgctatcat tgcagatagg atgaatttgc catttgctta tatccgtagc
     4561 aaaccaaaag atcatggtgc agggaatcaa ttagagggtc gcatcgtcaa gggacaaaaa
     4621 atggttgtcg ttgaagacct gatttctact ggagggtctg ttctagatgc cgtagcagca
     4681 gctgagcgtg aaggcgctga cgttattggt gttgtggcaa tctttaccta tgaattgcca
     4741 aaggcagatt gtaattttga taatgctggc gtgaaattgg tgacgcttag caattattca
     4801 gagttaatca aggtcgcaaa agtacaggga tacatcaatg cagatggttt gaatctgctt
     4861 aaaaagttcc gtcaaaatca agaaacttgg caggaagact agatttaaaa cgagcccacg
     4921 cacgatttct gggaacttat cctgtaaaat ggtataatag tctctaacgt attttgagaa
     4981 tacaataatt gaccgtatga ggagaagaac atgatttcag ttatcgtgcc ttgttttaat
     5041 gaagaagagg caattcccta tttttacgat gctatggaga aagttcgtaa ggagatgggg
     5101 gaacaatttg agtatatttt tgtcaatgat ggttcaaagg ataggacatt aacggtttta
     5161 cgccaattgt ctggtcaaga ccgagctgtt cgctatcttt ccttttcaag gaatttcggg
     5221 aaagaagctg ctctctatgc aggtctacaa gctgcgcaag gtgagctagt gactgtcatg
     5281 gatgttgatt tacaagatcc acctgaaatg ttgatggaaa tgaaggctat gttggatgga
     5341 aatccagatt tagattgcgt tggaacccgt cgtgtcagtc gagatggtga gccaccgatt
     5401 cgtagctttt ttgcaaaatt attctacaaa ttgatgaata aaatcagcca ggtcgaggta
     5461 gtagacggtg cccgtgattt ccgtctcatg cgtcgtcaca tggtagatgc cattctatct
     5521 gtgtctgaat acaatcgctt ttcaaaagta atttttgctt gggtaggatt cgaaacagaa
     5581 tacttgccct atgaaaatgt tgagagggtc gctggcgaaa ctagttggtc tttctggaaa
     5641 ttgctttctt attctattga aggaatcatc aatttttcag atacaccttt gaatattgcc
     5701 tcctatacag gtttcttcac cttcctattg tcgctagttc taatggtttt tgtggttttc
     5761 aaaaccttgg tgttcggaga tcctacaatt ggctggccgt cgactatttg tatcatccta
     5821 tttttgggag gtctgcagtt gatgaccatt ggtattctcg gtaaatattt ggcaaaagtt
     5881 ttcttggaaa cgaagaaacg tccgatttat attgttaaag agaaaagttc aaactaggag
     5941 cagtttatgg aacattcagc ttggcacgaa ttgataaagg cacagttgcc agagcattat
     6001 tttggtaaaa tcaatcagtt tttagattat gtttatgctg gtggaaccgt ttatccgcct
     6061 cgtgagaagg tatttgctgc cattcaaaca acggatttgg aagaggttaa agtagttatt
     6121 ttgggacagg atccctatca cggacctggt caggcacagg gcttgtcatt ttctgttccg
     6181 aactcggtgc cagctccgcc atctcttcaa aacattttaa aagaattgac ggatgatatt
     6241 ggcgtcaaac aagatcatga tttgacagca tgggcggaac aaggtgtgct tttactcaat
     6301 gcttgtttaa ctgttccagc aggtcaggca aatggacatg cagggcaaat ctgggagcct
     6361 tttacggacg ctgttattaa ggtggtcaat agcttagacc agcctgttgt ctatattctt
     6421 tggggtgctt atgctcgcaa gaaaaaagcg ttgataacca acccaaaaca tctggttatc
     6481 gaatctgctc atccaagccc tctttcagcc tatcgaggtt tctttggagg caagcccttc
     6541 tcacaagcaa atgcctactt gacttcacaa ggcagaacag gaattgattg gctgcgatag
     6601 tagatggtaa tggctttggt actacaaagg agaatatgaa tgttattagt gaaaaatggt
     6661 cgtgtattgg atccgcaatc tggtttagac aaggtttgcg acatcttgat ggacggaagt
     6721 gagattgtta agattgcgga gtcgatttca gaagatgttg agcaagaagt gattgatgcg
     6781 acaggattag tcgttgctcc tggtttggtc gatgttcatg tgcattttcg ggagccaggt
     6841 cagacccata aagaagatat tcatacagga gctctttctg cagcagcggg tggttttact
     6901 tctgttgtca tgatggctaa cactaatccg acgatttcaa atgttgaaac cttaaaagaa
     6961 gtattggatt ccgctaaaaa agaagctatt cacatttata gtgtggcaac cattacagat
     7021 catttcgacg ggcaaaatct gaccgatttt cctgctttgc tggaagctgg agcggtggcc
     7081 ttttcggacg acggtattcc tttgacaaat gctggcgtgg ttagaaaagc tatgaaattg
     7141 gccaaagagc agggctgtct catcagtctg catgaagaag accctgacct gaatggtgtc
     7201 ttgggactca acgagcaagt ggctgaaaaa cattttcatg tttgtggagc aacaggcgta
     7261 gcagaataca gcatggtcgc tcgcgatgtc atgattgcct atgaaacagg tgcgcgtgtt
     7321 catatccagc atctatccaa agccgagtcg gtcaaggtgg ttgaatttgt tcaaggtctc
     7381 ggagcaaacg tcacggcaga agtagcaccc caacattttt cacgaactga ggacttactt
     7441 ctaataaaag gtgccaatgc taaaatgaat ccgccactac gattagaaag tgatcgcttg
     7501 gctgtgattg atgggttgaa atctggtgtt atctctgtca ttgcgacgga ccatgcacct
     7561 catcatgcag atgagaaaaa tgtagaggac cttacgcaag ctccgtcagg tatgacaggt
     7621 ttagaaacct cactttcatt aggtctaact catctggtag aagatggaca cctcactctc
     7681 ttgcaattgc tggagaagat gactattaac cctgccaaac tctacggcct tgatgctggc
     7741 tatcttgctg aaaatggccc agctgatctt gttatttttg acccaaatgc aaatcggttg
     7801 gtcacagcgg attttgcctc gaagtctgct aattctccgt ttgttggtga tagtctgaaa
     7861 ggacaagtcc tttacaccat tgcaaatgga caaatagtat ttggtaaata ggaaatgagc
     7921 ggattagata gctaattcgc ttatttcgaa cctttttgac atgtaagcga ttttaatgtt
     7981 tttgtttttt gataaaaagt gtataataga gatataaaaa atgccgaaga aaggtctatt
     8041 tatgaaaaag aaaaaaatct tgttgcctgt aatgtccaca ctcttgttgg ctccatttgt
     8101 tttagctcaa caagtacaag ctgctgagac aacgactgca gctaccacaa ccaatcagcc
     8161 agcaacgaca gatgcaactg ctacggtacc agcaacatct gttgaaaatg tagcaacgga
     8221 ggaaactgtt gtccctgctg cggaagaaac agtagaggca gtcattatcc acacaaacga
     8281 cgttcacggt cgcattcttg aagaaaagaa tgttatcggt gacgctaagg ctgcggcagt
     8341 cattgaagaa gaacgtgcca aagtagaaaa tacaattgta gtagatgctg gtgatgcctt
     8401 ccaaggactt ccaatttcca actctacaaa aggtgaagac cgcgccaata tcatgaacca
     8461 agtcggctat gatgcaatgg ctgttggtaa ccatgaattt gactttggga tggatcaagc
     8521 aatcaagtac aaggaaacct tgaacttccc gctattgagt gctaacactt acgttaatgg
     8581 cgctcgtgtt tttgaagctt caactattgt cgataaaact ccaactgttg taggcgatga
     8641 atttgtagtt atcggtgtaa caacacctga aactgctaca aaaacccatc cgaagaatgt
     8701 tgagggagta acgtttaccg acccagtcac agaagtcaat aaagtgattg acgaagtgga
     8761 agcacgtgct ctagcagata atcgtgttta caagaactat atcattctag ctcacctagg
     8821 tgtcgattct acaacacctg ttgaatggag aggttcaact cttgctgaag cactttctaa
     8881 aaatagtaaa ttagccggta aacgtgttat tgttatcgat ggtcattccc acacggtaga
     8941 agcgactacc tatggagata acgttactta taatcaaaca ggctcttact tgaacaatat
     9001 tggtaaagta accttgaaat ccgataaatt attgggtgaa gctagcttga tttctgccgc
     9061 agatactaag aacgtgacgc caaatgctaa aatcgcagcc ttggttgatg aaattaaagc
     9121 aaaatatgaa gctgaaaatg ctcaagtagt tattgaaaat aacccagtgg aattaaacgg
     9181 agaccgctca aacgtgcgtg ttcgtgaaac caacttgggg aatgcagtga cggatgcgat
     9241 ttatgcctat ggtcaaacag gattttcaaa caaaactagc cttgctgtga ctaacggtgg
     9301 tggtctacgt gcaacaattg ctaaagatca gcctgttaca aaaggtgata ttattgcagt
     9361 cttgccattt ggtaacattg tttctcaaat cactgtgact ggtcagcaaa tttatgatat
     9421 gtttaccaag tctttatcat ctacattgca agtgaatcca gaaactggcg agatgctttt
     9481 agatgaaaat ggcatgccgc tctttgaagc tagtggtgga ttcctacaca tttcaggagc
     9541 aaatgtattc tacgatccaa ctcttcctgt agaagaacgt gttcttctta tcggtattct
     9601 taatccagag actggcgaat atgacgcctt ggatcttgaa aaaacgtact atttagcaac
     9661 aaatgacttc cttgctgctg gtggtgatgg ctacacaatg cttggcggag cacgtgaaga
     9721 aggtccttca atggacagcg tctttgctga atacctaaag acagctgact tatcagctta
     9781 tgaggttgtc aacccatatt cacgtatcat tcctgtaaat tcaagcattg atacggatga
     9841 agatggctac ccagacttta tcgaaatttt attggatact gatccagaaa atccagcctc
     9901 aaatccagaa actgttccag ccgaaaatac ggattcacca tctaatcaag tacaaaatac
     9961 ttcagcaacg gacaagaaag ctccagttga ttctccgaaa gtaggagata aaaaaacaga
    10021 agtagcaagt ccagtaaaaa ctacaaaagc aggagtcttg ccaaatactg gtgatcagat
    10081 gaatctgacg ctcagcctgt ttggtttagg tcttgcagga ctggccatgg cagtaggtcg
    10141 tagaaaagaa aattaatcaa tcatctgaaa acagggtctt cggactctgt ttttgactgg
    10201 aggaaacatg caaactgtat ttcaatatca aaaagagggg gatcgtgagc agattagttc
    10261 ttacgttctg cttagcaact ccccacgtcg taaacaatta ttgggatttt tacaaccaca
    10321 aatcgcatca gttgaagtgg atgagcgtgg tatcgaagaa cattttatga aacaattcgc
    10381 tacagatgat tttctcactc gagctgcaaa aacctgttgt gaaatttcca aagccaaatc
    10441 ggacatggat ttggtaactg gtcatctcta catttctgcg gatacaatcg tcgtgtcaga
    10501 tgggcaaatt tttaacaaac ctcaagattt gacagaagct gaaatcatgt tacgttccta
    10561 ttttggaaag agtcatcacg ttgtaacatc tgtctgccta cggacaagac actcactgga
    10621 tgttttttat acggttgcac aaattgattt tgtcgactat tatccagccc ttgaagaggt
    10681 tattcaatca tatatccgtg aaaagcagcc actcgacaag gcaggtgcat acggtattca
    10741 ggagctggat ccacgctttg tgtcgggcat ttcaggggat attcacacca ttattggtct
    10801 acctgtagcc gaagtttccc gcaggatttt ttctgaagaa ggttttgaaa aatagcagag
    10861 ctcaatagct agtttgtggt ataatagtgg tcgaaaagga gagtttatga ccgtcgacat
    10921 tgaaagatac aagcaattgg cccagcagaa gcaaggagaa catcgaaaat ttctagcaaa
    10981 tttgaagaaa aaagcaccta aaaatttgga tagaattgtt caagatattc atgccgaagt
    11041 attttctgaa atcgactgta cagagtgtgt caactgttgt aaaacgttgg gaccactctt
    11101 tacagaagca gatattaccc gaattgccaa gcatttacgc atgaaacttc cagtttttga
    11161 ggatatgtat ttgcgtgtgg atgaggacgg agataaggta tttcaatcca tgccctgtcc
    11221 cttccttgga ggcgataatc tatgtagtat ctatgatgct cgtcccaaag cctgtcgaga
    11281 atttccgcat accgaccgca agaagattta tcaaatcaac aatttaacaa taaaaaatac
    11341 tctgatttgt cccgcagcct atctctttgt cgaaaagtta cgggagagaa tttaagagga
    11401 acaagtaaga caaaatattg attttctgct ataataataa aaaaacaagc cgtttttacg
    11461 gcttttttca atgaatcata tttaggagga ccccatggca acaattcaat ggtttccggg
    11521 ccacatgtca aaagctcgca ggcaggtgca ggagaacatc aagcacgttg atttcgtgac
    11581 cattttggtc gatgcccgtc tgcccctatc cagccaaaac cccatgttga ctaagattgt
    11641 cggtgacaag cccaagctca tgattctcaa caaggtcgac ttagcagacc ctgttcgtac
    11701 caaagaatgg cagagctact ttgaaggtca aggcatccgt acccttgcca tcaactcaaa
    11761 agagcaagcg acggtgaaaa aggtgaccga cgcagccaag agtctgatga aagacaagat
    11821 tgaacgcctc cgcgagagag gtattcaaaa agaaaccttg cggaccatga ttatcggtat
    11881 tccaaacgct ggaaaatcca ctcttatgaa ccgtctggct ggaaagaaaa ttgccgttgt
    11941 tggcaacaaa ccaggtgtga cgaaaggcca acagtggctc aagtccaata aggaccttga
    12001 aatccttgat acaccaggta ttctctggcc taagtttgaa gaccaggaag tcggtctcaa
    12061 gttggctctg acaggagcta tcaaggacca acttttgcct atggacgaag tcaccatttt
    12121 tggtttggac tatttcaagg ccaactatcc agaacgtcta caagagcggt tcaagggcat
    12181 ggatttggag caggaaacgc ctgaactcat catggactgg accaagaaat tgggctttcg
    12241 tgatgattat gaccgttttt acaacctctt tgtcaaggac gttcgggacg gtcgcttggg
    12301 tacttataca ttagacaggg tgagtgactt agatggcaac gattaaggaa gtgactactc
    12361 tgctagctca ggtggacagt ctggacagtc ctgtctgggc ggaactatct caggatgagc
    12421 gagcaggggt ccaggctgcc atcaagaaac gccgtaagga gctggataaa gaagtagcag
    12481 aagatgcccg cttagaagct atgctatttt acgaaaaaaa cctttatgaa aatggggtgg
    12541 agttcatcgc tggtatcgac gaagtcggcc gggggcctct agcaggtcct gtggtggcag
    12601 cggcggtcat cttgcctaaa ggttgtaaaa tccgctacct caacgactcc aagaaaatcc
    12661 ctaagtccaa gcatgaagca atctaccaag aagtcatgga gcgagcggtg gcagtcggtg
    12721 tgggaataaa agatgcagct gtcattgacc aggtcaatat ctacgaggcg accaagttag
    12781 ctatgttgga ggcttttggg aaattaagcc agaaacctga ccacttgctc atagacgcca
    12841 tgaagttgga tacgcccata ccacaaactt ctatcatcaa gggagatgcc aatagcctat
    12901 ctatcgcagc tgcctctatc gtggccaagg tgacgcggga taagatgatg gcggactatg
    12961 acaaggagtt ctcaggctac ggcttcgcca agaatgctgg ttatggaacg gcagagcatt
    13021 tggaaggctt gaacaagtta ggcatcacgc ctattcaccg taagaccttt gagcctatta
    13081 agtctatggt aacaggaggg aaatcgtcgt gacggtcttt ttctggattt ttctagcttc
    13141 cataggctta tttggtctcc tatatttctg ggagcgacgg tcgattttta tctcactgtt
    13201 tgccttgaat atcctcgtct gggccttggc atggattatc tcaacaggca ttctcgaaaa
    13261 caatgaaatc ttgcgcttgc tggctatagc agtggctatg gtgctctttt tagccttctt
    13321 aagtggtcca tttgtcttgc ttttcacact ttatctaaat ggttttcaga ttttggagag
    13381 agaaggggtt cgcttccata atttcctatc catgggactg gcggtagccc tgactttcta
    13441 tctcttcatc gctccctttg tggttcaatc cctgtctggt attagtttgt taaacatggt
    13501 gtttgtctat gttggatttt tagtgtctta tgccattatc atcagcatgc tctataccac
    13561 gtctagcttt gtcaattttg tcaatctctt cccaggaaag cttgattatg tagtggtctt
    13621 aggtgctggc ttgattggtg acaaggtaac gccacttttg gcttcgcgga ttgacaaagg
    13681 catagcgatt tatcagaagc agcctggcag taagctcatc atgtcaggcg gacaaggtcc
    13741 tgatgaactc atagctgaag gtcaagctat ggcaaattat gccttggaac agggtatccc
    13801 tgcggaggac atcattattg aaaaccaatc gaccaacaca gaagaaaatt tgaaattttc
    13861 ctatgccctg atgaaacctg gcagtcgatt tgcacttgta accaattact atcacgtttt
    13921 cagagctctg ctattggcac gacaattgaa aattaaatgt atcggctacg gcgcaaaaac
    13981 caaattttac tttagcctca atgcctttat tcgggaattt gtaggctatg tagtcatgag
    14041 ccgaaagacg catttggtct ttttgagcat cgtgtcagcc ctttacctga tcggagtgat
    14101 catcagttta atgcattaaa aacagcctct aggtgactag aagctgtttt cttataacat
    14161 aaagtagaca atggcgatat attgaagtgc cgaagctagt aggataaaga gatgccaaat
    14221 catgtgaaag tagggctttt tcttggcgta gaagagggct ccaatagtat aagataatcc
    14281 accagccagc atgagaatgc caaatgccat tccagtcttt tgaagaatgg ctggcaagat
    14341 aaagacaact agccagccca tgaaaagata gaggaagagg ctgaattttt cgtttatttt
    14401 tttagcaaaa attttataga gtataccgaa aatagtcaat ccccattgca aacctacaat
    14461 cagataacct agccaaccac cgaccaagga tagggcaaca ggtgtgtagc ttccagcgat
    14521 agcaatgtaa atcatgctat ggtcgataat cctcaggatg tatttgtgag tagaagcata
    14581 gggcatggag tggtagatgg aagaggatag gaacatgaga aataggctga tgacaaagat
    14641 ggacatacca atcacagcgg tcaggttata atcttgatag gaatgaatag cagtaacggg
    14701 gagcaagacc aacataagca gggctccgac tgcatgtgtg acactgttag caacctcctc
    14761 tccaaaggat agttgcaggc tcaatttcaa actaggattc atggtgactc ctttctaact
    14821 ccatagtgag ttgacgggtt ttgtagtaaa gtttgacaga ttgacaagcg tagcgaatga
    14881 tgtcaggggt ttcttcattt tcctcgtttc gcatgcagtc aacaaaatga ttgtagagtg
    14941 tttctgaatg actcgattgg agaagaagat gtaaggtctg actgatttct tgaatagaaa
    15001 agacattttt taagatagtc aaggcaatca agcgggcaac ttgctgtttt tgatattttt
    15061 tcttaacagg tttttcaatc tgtttatgtt tgacatagtt attgatcatg gcagcagtca
    15121 atactcttcg aaaatcaaca ttatccgttg tcaacttgcc ttgatgaacc ccagttctat
    15181 catcggtttc gtttcctagg ctactgttga ttttcattga gtataaactt ttctgttcaa
    15241 ggctttcctg agaattgact acttgattaa catacagcag aacttggtcc aaatacaaat
    15301 ctaaattagg taattcatgc cagtagggga tggtctgttt catgattatc cactttcttt
    15361 tatctagtaa tgataactag attgtacgat atccaatatt aaaagtcaag aaaaaacctt
    15421 ctggacccaa aatcacagaa ggttagttgc ttatggttgg ataatatttt ctggtaaagc
    15481 catcttatag agtgaaaagg caccatctag gtttttaaca ccaaagccct tttctttcaa
    15541 gattcgttcg gcaatataag aacgaagtcc actatggcag atgacaatgt attcttttga
    15601 agcatccagt tctccaattc gctcacggag ttggtccaaa ggaatgtgaa tgaattctct
    15661 gaattgccca tttgcgacct caccaggatt gcggacatct aatagaactt taccagtagc
    15721 taactcgtcg tctagttgat gccactggat gttttggcta atcccttctg tgatgttcat
    15781 agcagcgtag ccaatcatat tgacagggtc tttggcagag ccaaatggcg gagcgtaggt
    15841 caattctagc tctggtaaat caaaaattgt cagtccagcc ttgatagcag tggcaaggac
    15901 atcaatccgc ttatcaatac ctttttgacc gacggcttgc gctccgaaaa tttttccatc
    15961 agtcggtgag aaaatgagtt ttaaaacaat atcagtcgca ccaggataat aacctgcatg
    16021 gtcttttcca gttgtatgaa taatggcatg ctcatagccg agtgattgaa cagtacgttc
    16081 gcttaaacca gttgaggcgg cagctaggtc aaagacttta acgatagctg ttccaataga
    16141 acctttgttt tttctatcaa gtccagcgat aacatccgcc acctgtctgc cttgtcggtt
    16201 ggcaggagaa gccagagaaa tcaaggcatc ttgacctgta atttcttgtt taacaacaat
    16261 agcatcacca acagcataaa tattattctg actggtttgg tagtttttat ctacgagaat
    16321 accaccacgg agacccagtt caattccagt ttttttagcc agttggctgt cgggagcaac
    16381 tccgactgag agaatggtca catcagatga caaggtttgt ccgttagaca attctagaac
    16441 ctgtcctttt tcatgaaaag cagtaacaga ctgcccagtg ataacctcga tacctttatt
    16501 ggttagctct gcttccacat atcgtgccat ttctaagtca aaagtaggta aaacatgggg
    16561 agcttgttcg acaagcgtga ccttccagcc ttttaaagct aagttttccg ccatttccag
    16621 accgataaaa cctgcaccaa ttaccacaac atgaccagac ttgatctggt ctagtttaat
    16681 cataatcttg tccaaatcag gtacgttccg aagagtaaag agattgtttg cttcttcaat
    16741 accagataga tttggaatga caggcttggc gccgggtgat aagattagtt tgtcgtagct
    16801 ttcaatcaac tcaccagtag gcgttttgac tgttacagtt tgctgctcag catcgatgct
    16861 aataacttca tggaaggggc gtacatctag attaaagcgt tgtttcaaac tttctggcgt
    16921 ttggaggagc aagctatcat gttctgctat ttcaccagaa atgtaataag gtaaaccgca
    16981 gttggcaaag gaaacataag gtcctttttc gaatacgatg atttcagcat cttccataag
    17041 acgacgcaga cgagtagctg ctgacatacc accagcgaca ccacctacaa taattatttt
    17101 catgctattc ctcctgtcca ggcattcata ccgcctttaa tgttgattgc ttgatagcct
    17161 tgagatttaa gatattctgt cgcccgttgg ctacgcatgc cagattgaca gatgacaaga
    17221 tagttcttgg ttttgtcgcc tttaaattgg tcaagttgac ttaaaggctg gttgatagct
    17281 tgtggaatat gtccactagc aaattcgtcg acttcgcgaa catcaagcaa aatagcattc
    17341 gatgattgta tcgcttgttc tacatcagtt atcggaagac tatctgagtt tgaaaacatt
    17401 cctgaaaata attgtttaaa cattgttact ccttt
//
LOCUS       DBNFEG010000023        17427 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.023, whole genome shotgun sequence.
ACCESSION   DBNFEG010000023 DBNFEG010000000
VERSION     DBNFEG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 17427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17427
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.023"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            8..81
                     /locus_tag="AC1PNG_000547"
     tRNA            8..81
                     /locus_tag="AC1PNG_000547"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Met,seq:cat)
     gene            98..171
                     /locus_tag="AC1PNG_000548"
     tRNA            98..171
                     /locus_tag="AC1PNG_000548"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:132..134,aa:Ile,seq:cat)
     gene            178..267
                     /locus_tag="AC1PNG_000549"
     tRNA            178..267
                     /locus_tag="AC1PNG_000549"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:214..216,aa:Ser,seq:tga)
     gene            278..351
                     /locus_tag="AC1PNG_000550"
     tRNA            278..351
                     /locus_tag="AC1PNG_000550"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:312..314,aa:Met,seq:cat)
     gene            367..439
                     /locus_tag="AC1PNG_000551"
     tRNA            367..439
                     /locus_tag="AC1PNG_000551"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:400..402,aa:Phe,seq:gaa)
     gene            471..544
                     /locus_tag="AC1PNG_000552"
     tRNA            471..544
                     /locus_tag="AC1PNG_000552"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:505..507,aa:Ile,seq:gat)
     gene            557..644
                     /locus_tag="AC1PNG_000553"
     tRNA            557..644
                     /locus_tag="AC1PNG_000553"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:591..593,aa:Ser,seq:gct)
     gene            704..1540
                     /gene="mreC"
                     /locus_tag="AC1PNG_000554"
     CDS             704..1540
                     /gene="mreC"
                     /locus_tag="AC1PNG_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIP6701077.1"
                     /translation="MNKFSKLVVVVSIFLLLSFSLLFVTFSKGLQVPYLNNIVRVVVT
                     PIQSVISVPTRFFSEQKDVLTDLMNAYEENKQLKEAIMSLEGMAAENTSLKEENASLR
                     SSLGVVSDFPEKQLIPGSVLVRTPSSWSEHISINIGETRGVTSNALVVANGGLVGIVS
                     SLSSDSAVVTLFTNSDEFTKLPVKISVDSKEIYGILSGYDADTNSFIINQLNSADEIA
                     VGSNVVTSDLAGATPANVQIGKVLSVKSNSNSLNREVYVEPTASFSNIYSVLVVGQTN
                     AQ"
     gene            1542..2045
                     /gene="mreD"
                     /locus_tag="AC1PNG_000555"
     CDS             1542..2045
                     /gene="mreD"
                     /locus_tag="AC1PNG_000555"
                     /inference="COORDINATES: protein motif:HMM:NF016019.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIP6701078.1"
                     /translation="MIEIFMFPILFFILLLDGQISTLVTNWSVGLFTISSHLVLMLAI
                     FYANYVSLGFSLFIFTLLGLVYDISYLNLIGIATTTLPLVLYCIYFFFQGAVSKRGIN
                     ILILLVAIFQFEFISYSFARIFHITNLSVFIFVFNKLLPSLLFNLVLFFVLQPLFERL
                     FGITNKT"
     gene            2130..3386
                     /gene="pcsB"
                     /locus_tag="AC1PNG_000556"
     CDS             2130..3386
                     /gene="pcsB"
                     /locus_tag="AC1PNG_000556"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan hydrolase PcsB"
                     /protein_id="HIP6701079.1"
                     /translation="MKKKILATIMLSTVVLSNANYVAVISANDVDSQIAAKNQQISEL
                     TAQQAEAQQQVDAIQGQVDAIVSEQAKLTEENTRLEAESQTLAADIERLSADIVSRDG
                     ALKEQARSAQVDGSASSYINTILDSKSIIDAVSRVNAMREIVSANNRMLEQQKADKEA
                     IVEKQKANQEAITTLAANRQKLEDDAQVLQVRQAELEAAKLNLAVQKATAEDEKNSLL
                     VQKAAAEEAARQAAARQAEYQAQQAALAQQQVASVSAPVVSTPVETTVTETFAAPTQT
                     VSQSTPTVSTPTTSTSSGSGSSAAANNARYDASSYPVGECTWGVKSQLSWVGPYWGDA
                     KQWLASARAEGFSTGSTPQVGAIAVWTGGYYGHVAVVTAVQSSTSIQVVESNYMGRRY
                     IGNHRGGYFNPTTTSEGAVYYIYPPY"
     gene            3489..4454
                     /locus_tag="AC1PNG_000557"
     CDS             3489..4454
                     /locus_tag="AC1PNG_000557"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009731949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIP6701080.1"
                     /translation="MAFTDLKLFALSSNQKLAEQVSKKIGIPLGKSSVRQFSDGEIQV
                     NIEESIRGTHVYILQSTSSPVNDNLMEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLQTAGVNRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGDGYVVVSPDHGGVTRARKLAQFLKTPIAIIDKRRSVDKMNTSEVMNIIGDIKDK
                     TCILIDDMIDTAGTICHAADALADAGATAVYASCTHPVLSGPAMDNISKSAIKKLVVL
                     DTIEIAEDRLIDKIEHISTAELLAEAIIRIHEKRPLSPLFEAKSC"
     gene            4545..5723
                     /locus_tag="AC1PNG_000558"
     CDS             4545..5723
                     /locus_tag="AC1PNG_000558"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIP6701081.1"
                     /translation="MDLLNRFNKNLNRIEVSMIRQFDQSISDVPGILKLTLGEPDFTT
                     PDHVKEAAKAAIDANQSYYTGMAGLLELRQAAAEFVAEKYNLHYNPENEILSTIGATE
                     ALSASLVAILEAGDTVLLPAPAYPGYEPIVNMVGADIVEIDTTANDFVLTPEMLEEAI
                     IEQGDKLKAVILNYPANPTGVTYSREQIQAFADVLRNYPVFVLSDEVYAELTYTGQPH
                     TSIAEFLPEQTILIQGLSKSHAMTGWRIGLIMSQAPIIAQIIKSHQYLVTAASTAMQY
                     GAVEALKNGKDDALPMRAEYVKRRDYIIEKMTDLGFKIIKPDGAFYIFAKIPAGYNQD
                     SFSFLQDFARKKAVAFIPGAAFGQYGEGYVRISYAASMEKIQTAMACLKEYLEENGTN
                     "
     gene            5710..6492
                     /gene="recO"
                     /locus_tag="AC1PNG_000559"
     CDS             5710..6492
                     /gene="recO"
                     /locus_tag="AC1PNG_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIP6701082.1"
                     /translation="MERIETRGLVLYNRNFREDDKLVKIFTEKAGKRMFFVKHASKSK
                     LVASIQPLTYADFIVKINDDGLSYIEDFHQVQPFKNINGDIFKLSYATYILALADAAL
                     QDKVYDPALFAFLVKTLDLMESGLDYEVLTNIFEIQLLGRFGISLNFHECAFCHRVGL
                     PFDYSYKYSGVLCPQHYQQDERRAYLDPNVPYLLDQFQAISFDELETISIKPEMKRKL
                     RFFIDQLYEEYVGIHLKSKKFIDDLSSWGQIMKPRTENEETE"
     gene            6489..7496
                     /gene="plsX"
                     /locus_tag="AC1PNG_000560"
     CDS             6489..7496
                     /gene="plsX"
                     /locus_tag="AC1PNG_000560"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIP6701083.1"
                     /translation="MKRIAVDAMGGDHAPQAMVEGVNQALAAFPDIEIQLYGDEAKIK
                     QYLTATERVSIVHTTEKINSDDEPVKAIRRKKDASMVLATKAVKDGQADAVLSAGNTG
                     ALLAAGVFVVGRIKNIDRPGLMSTLPTMDGKGFDMMDLGANAENTAHHLYQYGILGSF
                     YAEHVRGVKQPRVGLLNNGTEDTKGTPVHQETYKLLAEDKSINFIGNVEARELLNSVA
                     DVVVTDGFTGNAVLKTIEGTAKSIVGQLTGSIKNGGLRAKLGGLLVKPTLKKALGAMD
                     YKTAGGAVLLGLKAPVIKAHGSSDAQSIFYTIKQTRSILEAGIVEKSVAKFSVVEESH
                     D"
     gene            7489..7737
                     /locus_tag="AC1PNG_000561"
     CDS             7489..7737
                     /locus_tag="AC1PNG_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HIP6701084.1"
                     /translation="MTREQVYQRVVELIQDEKGEDFQVQPESTLADNIAADSVEIMEF
                     VLNLEDEFHVDVPDAAIEHFEVLSDIVDFIYEEAKKRS"
     gene            7855..8562
                     /gene="purC"
                     /locus_tag="AC1PNG_000562"
     CDS             7855..8562
                     /gene="purC"
                     /locus_tag="AC1PNG_000562"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIP6701085.1"
                     /translation="MKTDLLYSGKAKDIYATADSDQIVAVYKDQATAFNGGKKEQIVG
                     KGRLNNLISSLIFEKLNEAGVPTHFIKRLSDSEQLNKKVEIIPLEVVLRNVTAGSFSK
                     RFGVEEGITLPTPIVEFYYKKDELDDPFINDEHIAFLELASQDQIAYIKEETRRINEF
                     LKDLFAQIGLTLVDFKLEFGVDSSGQILLADEFSPDNCRLWDADGNHLDKDVFRRGLG
                     ELTEVYEVVLAKLQEVK"
     gene            8575..12294
                     /locus_tag="AC1PNG_000563"
     CDS             8575..12294
                     /locus_tag="AC1PNG_000563"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HIP6701086.1"
                     /translation="MAKRIFVEKKADFQIKAEALRKELTHNLQLTSLSSLRLVQVYDV
                     FNLEEDLLEQAIKHIFTEQVTDKVLSDEELGLEGAVYFAIEALPGQFDQRAASSQEAL
                     LLLGSRQEVRVNTGQLYILNGDVQEEELAAIKNYLLNPVDSRFKDMDAPLVTQEFSVS
                     DTTIPSLDFFDSYGVEEFASYKREAGLAMEVEDLLFIQDYFKSIGRVPTETELKVLDT
                     YWSDHCRHTTFETELRSIDFSASKFHKQLQATYDKYLAMRTELGRTDKPQTLMDMATI
                     FGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPFGG
                     AATCIGGAIRDPLSGRSYVYQAMRISGAGDITQPLTATRSGKLPQQIISKTAAHGYSS
                     YGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPVAGDVVILLGGKTG
                     RDGIGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGDVTRLIKKSNDFGA
                     GGVCVAIGELADGLEIDLDKVPLKYAGLNGTEIAISESQERMSVVVRPQDVDTFIAAC
                     RQENIHAVVVAKVTEKPNLVMTWNGQTIVDLERSFLDTNGVRVVVDAKVVDSSVKLPE
                     TRRTSAETLQEDLKDLLSDLNHASQKGLQTIFDSSVGRSTVNHPLGGRHQLTPTESSV
                     QKLPVQQGVTTTASVMAQGYHPYLADWSPYHGAAYAVIEATARLVATGANWSKARFSY
                     QEYFQRMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEDLTVPPTLVAF
                     GVTTADSCKVLSPEFKATGEHIYYLPGQALSEDIDFALIKSNFKAFEKWQSDYVITAA
                     SAVKYGGVLESLALMSFGNQVGARVELADLETSLTGQLGGFVFTSQEDIPDAVKIGQT
                     TTDFTLVVNGVNLSGRDLQVAFEGKLEEVYPTEFEQATELQDVPAVTSSAVIKAKETV
                     EVPVVYIPVFPGTNSEYDSAKAFEQAGAKVNLVPFVTLDAESIEQSVDTMVDNIAKAN
                     ILFFAGGFSAADEPDGSAKFIVTILRNAKVRSAIDQFIEKRGLIIGICNGFQALVKSG
                     LLPYGNFEKAGETSPTLFYNDANQHIAKMVETRIANVNSPWLAGVQVGDIHAIPVSHG
                     EGKFVVTDEEFATLRDNGQIFSQYVDFTGQPSMDSKYNPNGSSHAIEGITSRNGQIIG
                     KMGHSERYEDGLFQNVPGKKDQGLFVSAVRYFTGK"
     gene            12297..13751
                     /gene="purF"
                     /locus_tag="AC1PNG_000564"
     CDS             12297..13751
                     /gene="purF"
                     /locus_tag="AC1PNG_000564"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009908857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIP6701087.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     LANDGGQLRRHRGTGLIAEVFKNPADLEALTGTAAIGHVRYATSGSASINNIQPFLFD
                     FADMQVGLAHNGNLTNAVSLKAELEKNGSIFSSSSDTEILMHLIRRSHNPDFMGKIKE
                     ALNTVKGGFAYLILLEDKLVAALDPNGFRPLSIGKMKNGAWVVASETCAFEVVGADWV
                     RDVEPGEIVVIDDSGIQYDSYTRDTQLAVCSMEYVYFARPDSVIHGVNVHTARKNMGR
                     RLAQEFQHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSSVVKGKRVVMIDDSIVRGTTSRRIVQLLRDAGAKEVHVAIGSPELKY
                     PCFYGIDIQTRRELISANHTVDEVCEIIGADSLTYLSLEGMIEAIGIETDAPKGGLCV
                     AYFDGEFPTPLYDYEEEYLRSLEEKTSFYIENVK"
     gene            13807..14829
                     /gene="purM"
                     /locus_tag="AC1PNG_000565"
     CDS             13807..14829
                     /gene="purM"
                     /locus_tag="AC1PNG_000565"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIP6701088.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERLGVMGALGGFG
                     GMFDLTKLDVKEPVLVSGTDGVGTKLMLAIQYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYIATGKNEPAKLEQVVAGVAEGCVQAGCGLIGGETAEMPGMYGEDDYDLAGFAV
                     GIAEKYQIIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADVSGDALLPELNGRALK
                     DVLLEPTRIYVQQVLPLVKAGLVNGIAHITGGGFIENVPRMFADNLAAEIEEDKIPVL
                     PIFTALEKYGKIKHEEMFEIFNMGIGLVLAVSPDKVDSVCQLVDEEVYTIGRIIAKED
                     KSVVIK"
     gene            14826..15377
                     /gene="purN"
                     /locus_tag="AC1PNG_000566"
     CDS             14826..15377
                     /gene="purN"
                     /locus_tag="AC1PNG_000566"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIP6701089.1"
                     /translation="MKRIAVFASGNGSNFQVIAEQFKVAFVFSDRRNAYVLERAEKLG
                     VPTFAFELKEFSDKQTYEEAIIQLLDQHQIDLVVLAGYMKIVGPTLLAQYEGRIINIH
                     PAYLPEFPGAHGIEDAWNAGVAESGVTVHWVDSGVDTGQIIKQVRVPRLADDTLETFE
                     ARIHEAEYQLYPAVLEELGAVKK"
     gene            15387..16934
                     /gene="purH"
                     /locus_tag="AC1PNG_000567"
     CDS             15387..16934
                     /gene="purH"
                     /locus_tag="AC1PNG_000567"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIP6701090.1"
                     /translation="MTKRALISVSDKNGIVEFAQELTKFGWEIISTGGTKVALDQAGV
                     TTIAIDDVTGFPEMMDGRVKTLHPKIHGGLLARRDLDSHLQAANDHEIGLIDLVVVNL
                     YPFKETILRPDVTYDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYPTVLGEIAEQ
                     GETSYATRQRLAAKVFRHTAAYDALIADYFTKQVGEDKPEKLTITYDLNQPMRYGENP
                     QQNADFYQNALPTAYSIAAAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHM
                     NPCGIGQAETIEQAWDYAYEADPVSIFGGIVVLNREVDAATAEKMHPIFLEIIIAPGY
                     SAEALAILTNKKKNLRILELAFDAQDASEVEKEFTGVVGGLLVQDQDVVVESPVDWQV
                     VTERQPSEQEWAAMEFAWKSSKYVKSNGIIITNDKMTLGVGPGQTNRVASVRIAIEQA
                     KDRLEGAVLASDAFFPFADNVEEIAAAGIKAIIQPGGSVRDQDSIDMANKYGLTMVFT
                     GVRHFRH"
     gene            16999..>17427
                     /locus_tag="AC1PNG_000568"
     CDS             16999..>17427
                     /locus_tag="AC1PNG_000568"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - obsolete DNA methylation on
                     adenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HIP6701091.1"
                     /translation="MTLKPFTKWTGGKRKLLTQLHEHLPFEYNRYFEPFVGGGALFFD
                     LAPENAVINDFNEELINTYLQIRDNPEALLDLLHIHQENNSKEYYLDVRSVDRDGRIE
                     TMSDVERAARILYMLRVDFNGLYRVNSKNQFNVPYGRYKNP"
BASE COUNT         4746 a         3378 c         4361 g         4942 t
ORIGIN      
        1 ttattttggc ggtgtagctc agctggctag agcgtccggt tcatacccgg gaggtcgggg
       61 gttcgatccc cttcgccgct atatactctt gtttgctgga cctttagctc agctggttag
      121 agctctcggc tcataaccga gcggtcgtag gttcaagtcc tacaaggtcc atatattgga
      181 ggattaccca agtccggctg aagggaacgg tcttgaaaac cgtcaggcgt gtaaaagcgt
      241 gcgtgggttc gaatcccaca tcctcctttt ctactaccgc gggatggagc agctaggtag
      301 ctcgtcgggc tcataacccg aaggtcgtag gttcaaatcc tgctcccgca atttctttaa
      361 gaatttggct cggtagctca gttggtagag caatggattg aagctccatg tgtcggcggt
      421 tcgattccgt ctcgcgccat taatttaact ttgtccaagt ttattatctt gggcgcgtag
      481 ctcagatggt tagagcgcac gcctgataag cgtgaggtcg gtggttcgat tccactcgtg
      541 cccatattat attgatggag aattactcaa gaggctgaag aggacggttt gctaaatcgt
      601 taggtcgggt aactggcgca agggttcgaa tcccttattc tccgtagtga acgagattat
      661 cgcttgatat tctcgtttta ttgtatctta gtgaggatcc attatgaata aattttcaaa
      721 attagtagta gttgtttcta tctttttatt actgtcattt tctcttttgt ttgtgacttt
      781 ctctaaggga ttacaggtac catatttaaa taatatcgtt agggttgttg taacgccaat
      841 tcaatcggtt atttcagtac ctactagatt tttttctgag cagaaagatg tcttgacaga
      901 tttgatgaac gcttacgagg aaaataaaca attaaaggaa gctattatga gccttgaagg
      961 gatggctgct gaaaatacta gcttgaaaga agagaatgca tcgcttcgta gcagtttagg
     1021 tgtggtatct gattttcctg aaaaacaact tattccagga tcagttttag tgaggactcc
     1081 ttcatcgtgg tcggagcata tttcaattaa tattggtgag actaggggtg tgacatctaa
     1141 tgcacttgtg gttgctaatg gtggattagt tggcatcgtt agttcattga gttcagattc
     1201 ggcggttgtt accttattta cgaattcgga tgaattcacg aagttgcctg tgaaaatttc
     1261 tgttgattct aaagaaattt atggtatttt atcgggctat gatgcagata caaatagttt
     1321 tatcattaat cagttgaact cagctgatga aattgcagtg ggtagcaatg ttgtaacgag
     1381 tgatttggct ggtgcgactc cggcaaatgt ccaaattggt aaagttttat cggttaaatc
     1441 aaatagtaac agtttaaata gagaagtata tgttgagccg acagctagtt tttcaaacat
     1501 ttattcggtt ttagtggtag gtcaaacaaa tgcgcaataa aatgatagaa atattcatgt
     1561 ttcccatctt gttctttatt ttattacttg atggacagat ttcaacgttg gtaacaaatt
     1621 ggtcggtcgg attatttact atttcaagtc atttggtact tatgttagct attttttatg
     1681 ctaactatgt atctcttggt ttttcattat tcatatttac tttgctaggt ttggtatatg
     1741 atattagtta tcttaatctg attggtattg ctactacaac tcttccttta gttttatatt
     1801 gcatctattt cttctttcaa ggtgctgtca gcaagcgagg aattaatatt ttgattttac
     1861 tagtagctat ttttcaattt gaatttatta gttattcatt tgctcgtatt tttcatataa
     1921 caaacttgtc tgtatttatc tttgttttta ataaattact tccaagttta ctattcaatt
     1981 tggtcttatt tttcgtgtta caaccattat ttgagcgttt atttggaata acaaacaaga
     2041 catagaaatg taataattgc gtaatatact tacgcgattt ttttgataaa atgagagagt
     2101 cagaaagtat agaggagtta tgttcggata tgaagaaaaa aatcttggct acaattatgt
     2161 taagtacagt cgttctatct aatgctaatt atgtagctgt gattagtgcg aatgatgtag
     2221 atagtcagat tgcagcaaaa aatcaacaga ttagtgagtt gacagcacaa caagctgaag
     2281 ctcaacaaca agttgatgct attcaaggac aagttgatgc aattgttagt gaacaggcga
     2341 aattaacaga agaaaatact cgtttggaag cagaatcgca gacattggcg gcagatattg
     2401 agcgtttgtc agctgatatt gtgtcacgtg atggtgcttt aaaggagcag gcgcgtagtg
     2461 ctcaagttga tggttctgct tcaagttaca ttaatacaat tttggattca aaatcaatca
     2521 ttgatgctgt ttctcgtgtt aatgcaatgc gtgagattgt ttcagctaat aaccgtatgt
     2581 tagaacaaca aaaggctgat aaggaagcaa ttgttgaaaa acaaaaggca aatcaagagg
     2641 caatcactac tttggcagct aatcgccaaa aattggaaga tgacgcccaa gtattgcaag
     2701 tgcgtcaggc tgaattggaa gctgctaagt taaatttggc tgtgcagaaa gctactgctg
     2761 aagatgagaa aaattcattg ttggtgcaaa aggcagctgc agaggaagcg gctcgtcaag
     2821 cggcagctcg tcaagcggag tatcaagcac aacaggcagc tctagcacag caacaagtag
     2881 cctcagtatc agcaccagtc gtgtcaacgc cggtagaaac tacagtgact gaaacgtttg
     2941 cagcccctac tcagacagta tcacaatcta caccaacggt aagcacacct acaacgtcta
     3001 catcatcagg ttcaggtagt tcagcggcag caaataatgc gcgttatgac gcttcatctt
     3061 acccagttgg tgagtgtact tggggagtta aatctcaact ttcatgggtt ggtccttact
     3121 ggggagatgc taaacagtgg ttagcatcag cacgtgctga aggttttagt acaggttcta
     3181 ctccacaagt aggtgcgatt gctgtttgga caggtggtta ttatgggcac gtagctgttg
     3241 ttacggctgt tcaatcttca acaagtatcc aagttgttga atcaaactac atgggtcgtc
     3301 gttatattgg taaccatcgt ggtggttatt tcaatccaac aacgacttct gagggagcag
     3361 tttactacat ttaccctcca tattaagaaa ttttcaaata gagcgtgtgg atttgcgctc
     3421 tatttttgtt tgaaaaatga aggaaaaagg gttaaaatag taaaggtaga aatatatagg
     3481 aggctgtcat ggcgtttact gacttaaaat tgttcgctct ttcgtcaaat cagaagttag
     3541 ctgaacaggt gtcgaaaaaa ataggaattc ctctgggaaa atcaagtgtt cgtcaatttt
     3601 cagatggtga gattcaggta aatattgagg aatccattcg tggaactcat gtgtatattc
     3661 ttcaatccac tagctcacca gttaatgata atttaatgga aattttgatt atggtcgatg
     3721 ctcttaaacg tgcatctgct gaatcagtaa acgtagtaat gccttattat ggttatgcac
     3781 gccaagaccg gaaggctcgt gcacgggaac caattacatc aaaattggtg gctaatatgt
     3841 tgcagacagc gggggtcaat cgtttgttga caattgattt gcatgctgcg cagattcagg
     3901 gattcttcga tattcctgtt gatcatttga tgggtgctcc attgattgcg gattattttg
     3961 agcgtcgtgg tatggtaggt gatggctatg ttgttgtatc ccctgaccat ggtggtgtga
     4021 cacgtgcccg taagttagca cagttcttga agacaccgat tgctattatt gataaacgcc
     4081 gtagtgttga taagatgaat acatcagaag tgatgaacat tattggcgat attaaggata
     4141 aaacttgtat cttgattgat gatatgattg atactgcggg taccatctgt catgcagcgg
     4201 atgctttggc agatgcaggg gcaacagctg tttatgcttc atgtacgcac ccagtattgt
     4261 cagggcctgc tatggataac attagtaagt cagcaattaa gaaattggtt gttcttgata
     4321 caattgaaat tgcagaagat cgcttaatcg ataaaatcga acatatttca acggcagaat
     4381 tattagcgga agcgattatt cgtattcatg aaaaacgtcc tttgtcacct ttgtttgagg
     4441 caaagtcgtg ttaaataggt atgagaagag tcaaatgact cttctttttt acatttattt
     4501 tcaaaatgta ttataatagt tttatatgtt tttgaggtgg cagaatggat ttattgaatc
     4561 gttttaataa gaatttgaat cggattgagg tttcgatgat tcgtcagttt gatcagtcta
     4621 tttcggatgt tcccgggatt ttgaaactga ccttgggaga gcctgatttt acaacgcctg
     4681 atcatgtaaa agaagcagct aaggctgcta tcgatgctaa ccagagttat tatacaggta
     4741 tggctggtct tttggagttg cgccaagctg cagctgagtt tgtggctgaa aaatataatt
     4801 tacactacaa tccagaaaat gaaatcctct ctacaatcgg tgcgacagaa gctctgtcag
     4861 cgagtttagt tgctattttg gaggctgggg atacagttct tctgcctgcc cctgcctatc
     4921 ctggttatga gccgattgtc aatatggtgg gagcagacat cgttgaaatc gatacgacgg
     4981 caaatgattt tgtattgaca cctgagatgt tggaagaggc gattattgag cagggtgata
     5041 agctaaaagc tgtcattctc aactatcctg ccaatccaac aggtgtgacc tactcacgtg
     5101 agcagattca ggcttttgcg gatgtcttgc gtaactatcc tgtctttgtc ctatcagatg
     5161 aggtctatgc ggagttgact tacactggtc agcctcatac ttcgattgcg gagtttttgc
     5221 cagaacagac cattttgatt cagggattgt ccaagtccca tgctatgaca ggttggcgga
     5281 ttggcttgat tatgagtcag gctcccatca tcgctcaaat tatcaagagc catcagtatc
     5341 ttgtgactgc agcgtcaact gctatgcagt atggtgcggt tgaggccttg aaaaatggta
     5401 aggatgacgc tcttccgatg cgggcggagt acgtcaagcg tagggattac atcatcgaga
     5461 agatgacgga cttgggcttt aaaattatca agcctgatgg agcattttac atctttgcaa
     5521 aaattcctgc gggttacaat caagattcct ttagcttctt gcaagacttt gcgagaaaga
     5581 aagcggtggc ctttattcca ggcgcagcct tcggtcagta tggagaaggc tatgtgcgaa
     5641 tttcctacgc agcaagtatg gagaagattc aaacagccat ggcttgtttg aaggaatatc
     5701 tagaagaaaa tggaacgaat tgaaaccagg ggattagtcc tatataatcg gaattttcga
     5761 gaagatgaca agctggtcaa gatttttaca gagaaggctg gcaagcgaat gtttttcgtg
     5821 aaacatgcct ctaaatccaa gctggtagct tctatccagc ctttgaccta tgcggatttt
     5881 atcgttaaaa tcaatgatga tgggctgtct tatatcgaag attttcatca ggtacagccc
     5941 tttaagaata ttaacggtga tattttcaag cttagctatg ctacctatat cttggccttg
     6001 gccgatgcgg ccttgcagga caaggtctat gacccagctc tctttgcttt tttggtcaag
     6061 accttggact tgatggagtc aggtttggac tacgaagttc tgaccaatat ctttgaaatt
     6121 cagctcttgg gtcgatttgg gatcagtctg aattttcacg agtgtgcttt ttgtcatcgg
     6181 gttggcttgc cttttgacta ttcctacaag tacagcggtg tcttgtgtcc gcaacactat
     6241 caacaagatg agcgacgggc ttatctggat ccaaatgttc cctatctact tgatcaattt
     6301 caggctattt cctttgatga gctggaaacc atttccatca agcctgagat gaagcgaaaa
     6361 ttacggtttt ttattgacca gctgtacgag gaatatgtgg ggattcactt gaaatccaag
     6421 aaatttatag atgatttgtc ttcttggggg cagattatga aaccaagaac agaaaatgag
     6481 gaaacagaat gaaacgtatt gcagtagatg ctatgggtgg agaccacgcc cctcaggcaa
     6541 tggtagaagg tgtcaatcaa gccttggctg cctttccaga cattgaaatt caactttatg
     6601 gtgatgaggc taaaatcaag cagtatttga cagcgacaga gcgtgtcagc atcgtccata
     6661 cgacggagaa aatcaattca gatgatgagc ctgtcaaggc gattcgtcgt aagaaagatg
     6721 cttcgatggt cctagcgacc aaggctgtca aggatggtca ggcagatgcg gtcttgtcag
     6781 ctggaaatac aggtgctctt ttggcggcag gcgtctttgt cgtcggtcgc atcaagaaca
     6841 tcgaccgtcc tggtcttatg tctaccttgc cgactatgga cggtaaggga tttgatatga
     6901 tggatttggg ggcaaatgct gaaaatacag ctcatcacct ctatcagtat ggtattcttg
     6961 gctcatttta cgcggagcac gtgcgtggtg tcaaacaacc tcgtgtggga cttttgaaca
     7021 acggtacgga agataccaag ggcacgccag ttcaccaaga aacctataaa ctcttggcgg
     7081 aagacaagtc aatcaacttt atcggtaatg tggaggcgcg tgagttgctc aacagtgtgg
     7141 cagatgtggt cgtgacggat ggtttcacgg gaaacgctgt gctgaaaacc attgagggaa
     7201 ctgcaaaatc catcgttggt cagttgactg gctcaatcaa gaatggcggt ctgcgtgcta
     7261 aattgggagg cttgttggtc aaaccaacct tgaaaaaggc cttgggggct atggactata
     7321 aaactgcagg tggtgctgtc ttgcttggtc tgaaagcacc tgtcatcaag gcgcacggat
     7381 ccagcgatgc ccagtcgatt ttctatacga ttaagcagac gcgttcgatt ttggaggctg
     7441 gcattgttga aaaatcagtg gccaaatttt cagtagtgga ggagagtcat gactagagag
     7501 caggtctatc agcgggttgt tgaattaatt caggatgaaa agggagaaga ttttcaagtt
     7561 caacctgaat ctactttggc agataatatt gcagcagatt cagtagaaat catggaattt
     7621 gttcttaatt tggaggatga atttcatgta gatgttccag atgctgctat tgagcatttt
     7681 gaggtcctat ctgatattgt tgattttatt tatgaagaag caaagaaacg ttcgtagttt
     7741 acgggcgttt ttctttgttt tttcctaaaa aaccgtaaat ttgttgtgaa tgcgaatatt
     7801 gtgtgttaga ataaacgaaa agactagaat attttacgaa aggcgaacat tctcatgaaa
     7861 acagaccttc tctattcagg aaaagccaaa gacatctacg ctacggctga tagcgaccag
     7921 attgttgcgg tttataagga tcaggcaacg gcttttaatg gtggtaagaa agaacagatt
     7981 gtgggcaagg gccggctcaa taatctgatt tcatctttga tttttgaaaa attgaatgaa
     8041 gctggtgtcc caacgcattt tatcaagcgt ttgtcggatt cggagcagtt gaataagaag
     8101 gtggagatta ttcctcttga ggttgttttg cgaaatgtga cagctgggtc tttctcaaaa
     8161 cgcttcggtg tggaagaagg gataacctta cctactccta tcgtggaatt ttactataaa
     8221 aaagatgagt tggacgatcc ttttatcaac gatgaacaca ttgcctttct agaactggct
     8281 agtcaggacc aaatcgccta tatcaaggaa gaaacaagac gaatcaatga attcttgaag
     8341 gacttgtttg ctcagattgg gctgactttg gtggacttca agctagaatt tggggttgac
     8401 tcgtctggtc agattttatt ggcggatgag ttttcgcctg ataattgtcg tttgtgggat
     8461 gcggatggca accatctgga caaggatgtt ttccgtcggg ggctcgggga gttgaccgag
     8521 gtttacgagg tcgtattggc aaagttacaa gaagtgaaat aaggattgga aaatatggca
     8581 aagcggattt ttgttgagaa gaaggcggat tttcagatta aggcggaggc tcttcgcaag
     8641 gagttgactc ataatttaca gttgacaagc ctgtcaagtt tgcggttagt gcaggtttac
     8701 gatgtcttca atcttgagga ggacttgtta gagcaggcca tcaagcatat ctttacagag
     8761 caggtgacgg acaaggtttt gtcggatgaa gaattaggtc tggaaggtgc agtttatttt
     8821 gctattgagg ctttgcctgg gcagtttgac cagcgggctg ccagcagtca ggaggccctt
     8881 ctcttattgg gcagtcgtca ggaggtgcgt gtcaatacag gtcagctcta tattttgaac
     8941 ggtgatgtgc aggaagaaga attagctgcc atcaagaatt atttgctcaa ccctgtggat
     9001 tcgcgattca aggatatgga cgctcctttg gtgactcagg agttttcagt ttcggatact
     9061 actattccaa gcttggactt ttttgatagc tacggggtag aggaatttgc gtcttacaag
     9121 cgtgaggctg gtttggctat ggaagtggaa gacctgctct tcattcagga ttacttcaag
     9181 tcaattggtc gagtgccaac tgagacagaa ctcaaggtct tggatactta ctggagtgac
     9241 cactgccgcc atacgacatt tgagacagaa cttaggtcta ttgacttttc agcttcaaaa
     9301 ttccacaagc aattgcaggc gacttatgac aagtatctgg ctatgcgaac agagttaggt
     9361 cggacagaca aaccgcagac gcttatggat atggcgacga tttttggtcg ctatgagcga
     9421 gcaaatggtc gtctggatga catggaagtg tcagatgaaa tcaatgcttg ctcggtggaa
     9481 atcgaagtag atgtggatgg cgtgaaagag ccatggctcc tcatgttcaa gaatgaaacc
     9541 cataatcacc caacagaaat cgagcctttt ggtggtgccg caacctgtat cggtggagcc
     9601 attcgtgatc ctttgtcagg tcgttcttat gtttatcaag ccatgcggat ttcaggtgca
     9661 ggcgatatta cccaacctct gacagctact cgctcaggaa aattgccaca gcaaatcatt
     9721 tcaaaaacag cggcacatgg ttattcttca tacggaaacc aaatcggttt ggcgacaact
     9781 tatgtacgtg aatatttcca cccaggcttt gtcgcaaaac gtatggaatt aggtgccgtt
     9841 gtcggtgctg ctcctaagga aaatgtggtt cgtgaaaaac cagtcgcagg cgatgtggtt
     9901 atcttgttag gaggcaaaac aggtcgagat ggtatcggtg gggcaacagg ctcgtccaag
     9961 gtacagacag ttgagtctgt ggaaacagct ggtgcggaag tccaaaaagg aaatgccatt
    10021 gaagaacgca aaatccaacg cttgttccga aatggtgacg ttacacgcct gataaaaaaa
    10081 tccaatgact tcggtgcagg cggtgtctgc gtagctattg gggaattggc ggacggtctt
    10141 gaaatcgatt tggataaggt tcctctaaaa tatgcaggct tgaatggaac ggaaatcgct
    10201 atttctgaat cacaggagcg gatgagtgtc gttgttcgtc ctcaagatgt cgataccttc
    10261 atcgcagcct gccgtcagga aaatatccat gcggttgttg tagccaaagt gacggaaaaa
    10321 ccaaatctgg tcatgacttg gaatggtcaa accattgtcg acttggaacg ttccttcctt
    10381 gataccaacg gtgtgcgcgt ggtggtggat gccaaagtgg ttgacagttc tgtcaagcta
    10441 ccagaaacac gtagaacttc cgctgaaacg ctacaggagg acttgaaaga ccttctatca
    10501 gacctcaacc atgctagcca gaaaggtttg cagacgattt tcgactcatc tgttggtcgt
    10561 tcaaccgtca accacccact cggaggtcgt caccagctga ctccgacaga aagttcggtg
    10621 caaaagttgc ctgtccaaca gggtgtgacg acgacagcat cagtcatggc tcagggttac
    10681 catccttacc tagcagactg gtcaccttat cacggagcgg cttatgcagt catcgaagcg
    10741 acagctcgct tggtggcaac aggtgctaac tggtccaagg ctcgcttctc ctatcaggag
    10801 tatttccagc ggatggacaa gcaggctgag cgttttggtc agccagtagc ggctttgctt
    10861 ggttctatcg aggctcagat tcagcttggc ttgccgtcta ttggtggcaa ggactccatg
    10921 tctggtacct ttgaggactt gacagttccg ccgaccttgg ttgcctttgg cgtgaccacg
    10981 gcagacagct gcaaggttct atcacctgag ttcaaggcga ctggcgagca tatctattac
    11041 ctaccaggcc aagccttatc agaagatatt gattttgcct tgatcaagtc taattttaag
    11101 gcttttgaaa aatggcagag cgattatgtg attacggctg ctagtgcagt caagtatgga
    11161 ggagttctag aaagtctagc ccttatgtcc tttggtaacc aagtcggagc aagagttgag
    11221 cttgccgacc ttgaaactag cttgacaggt caacttggtg gctttgtctt cacatcgcaa
    11281 gaagacattc cagatgctgt gaaaatcggt caaaccacta cagactttac actggttgtc
    11341 aatggtgtca acctttctgg acgggacttg caagtagctt ttgagggcaa actagaagaa
    11401 gtttacccaa cagagtttga acaggcgacg gagttgcagg atgttccagc agtcacaagt
    11461 tcagcggtta taaaagcaaa agaaaccgtt gaagtgcctg tagtttacat tccagttttc
    11521 ccaggtacca actcagaata tgattctgcc aaggcctttg aacaggctgg tgcaaaagtc
    11581 aatcttgttc catttgtgac cttggatgca gagagcattg aacagtctgt ggacactatg
    11641 gttgacaata ttgctaaggc gaatattcta ttctttgcag gtggattctc ggctgcggat
    11701 gaaccagatg ggtctgctaa gtttatcgtg actatcttac gaaacgccaa ggtccgctct
    11761 gctattgacc aatttatcga aaaaagaggc ctcatcatcg gtatctgtaa tggtttccag
    11821 gcccttgtca aatcgggctt gttgccgtat ggaaactttg agaaggcggg tgaaaccagt
    11881 ccaaccctct tctacaacga tgccaaccaa cacattgcta aaatggtgga aacgcggatt
    11941 gccaatgtca actcaccgtg gttggcaggt gtccaagtcg gcgacattca cgctatccca
    12001 gtttcccacg gtgaaggaaa atttgtagtg acggacgagg aatttgctac tttgcgtgat
    12061 aatgggcaaa tcttcagcca atacgttgac tttaccggtc agccaagcat ggattctaag
    12121 tacaatccaa atggatccag ccatgccatt gagggcatta ccagtcgcaa cggtcagatt
    12181 atagggaaaa tggggcattc cgagcgttac gaggacggtc ttttccaaaa tgttccaggt
    12241 aagaaagatc aagggctctt tgtttcagca gttcgctatt ttacaggaaa ataaatatga
    12301 catacgaagt taaatcactc aacgaagaat gcggtgtttt cggcatttgg ggacatccgc
    12361 aggctgctca ggtgacctat tttggtctcc atagtttgca gcaccgtgga caagaaggag
    12421 cagggatttt agccaatgat ggcggacaat tgcgtcgcca tcgcggaaca ggcctgattg
    12481 cagaagtctt caaaaatcca gcagatttag aagccttgac aggaacggct gctattggtc
    12541 atgtccgcta tgcaacttct ggctctgctt ctatcaataa tatccagccc ttcctatttg
    12601 attttgcaga tatgcaggtg ggtttggcac acaatgggaa tttgaccaac gcggtcagtt
    12661 tgaaggctga acttgaaaaa aatggttcca ttttctcttc ttcttctgac actgagattc
    12721 tcatgcactt gattcgccgc agtcacaatc cagactttat gggaaaaatc aaggaagccc
    12781 tcaatactgt taaagggggc tttgcctacc tgatcttgtt ggaagacaaa ttggttgctg
    12841 ccttggaccc taacggtttc cgtccacttt caataggcaa aatgaaaaat ggtgcctggg
    12901 tagttgccag cgaaacttgt gcttttgaag tagtgggggc tgactgggtg cgagatgtgg
    12961 agcctggtga aattgtcgtc attgatgatt caggaattca atatgatagc tatacaagag
    13021 atacgcaact ggctgtttgc tctatggagt atgtctattt tgctcgccca gattcggtaa
    13081 ttcacggggt caatgtccac acggctcgta aaaatatggg tcgtcgtttg gcccaggaat
    13141 ttcaacatga agcagatatc gtggttggtg tgccaaactc ctccctatct gcggctatgg
    13201 gatttgcaga ggaatctggt ttgccaaatg aaatggggct gattaaaaac caatacaccc
    13261 agcggacctt tattcaacca acccaggaat tgcgggagca gggtgtgcgc atgaagttgt
    13321 cagccgtttc aagtgttgtc aaaggcaagc gtgtggtcat gattgacgac tctattgttc
    13381 gcggtacaac tagccgccgc attgtccagc ttcttcgtga tgcaggggca aaagaagtcc
    13441 atgtggctat cggcagccca gaactcaagt acccgtgttt ctatggtatt gatattcaga
    13501 cccgtaggga gctgatttca gccaaccata ccgttgatga agtctgtgag attatcgggg
    13561 cagacagtct gacctacctt tctcttgagg ggatgattga agccatcggc atcgaaaccg
    13621 atgcgccaaa aggcgggctc tgtgtagcct actttgacgg agaatttcca acgcctctct
    13681 atgactatga ggaagaatac cttcgtagcc tagaagagaa aacgagtttc tacattgaaa
    13741 atgtcaagta aagacagagg tgtcaacgtt acttgacaga cctgtaaact gttgaaagga
    13801 atcaaaatga caaataaaaa tgcctacgcc caatcgggcg ttgacgtcga agcgggatat
    13861 gaagttgtcg aacgcatcaa gaagcatgta gctcggacag aacgcttggg tgtgatggga
    13921 gccctcggtg gttttggcgg gatgtttgat ctaaccaaac tggatgtcaa agagccagtc
    13981 ttggtatctg ggacagacgg tgtgggcacc aagctcatgc tggctatcca gtacgacaag
    14041 catgacacca ttggtcaaga ttgcgtggct atgtgtgtca acgacattat cgcagcaggt
    14101 gcagagccgc tttacttcct tgactacatt gcgactggaa aaaatgagcc agccaagctg
    14161 gagcaagtag tagccggtgt tgcagaaggc tgtgtccaag ctggttgcgg attgattggc
    14221 ggtgaaacgg ctgaaatgcc tggtatgtac ggcgaggatg actatgacct ggctggcttt
    14281 gctgtcggta ttgcggagaa gtatcagatt atcgacggta gcaaggtcaa ggagggtgac
    14341 attctcctcg gcctagcttc cagcggtatc cactccaacg gatattccct agtccgtcgt
    14401 gtttttgcag atgtttctgg ggacgctctg ttgccagagc tcaatggcag agctcttaag
    14461 gatgtcctat tagagccgac acgtatctat gtccagcagg tgttgcctct ggtaaaagca
    14521 ggtctggtca acggcattgc ccatatcaca ggcggtggat ttatcgaaaa tgtgcctcgt
    14581 atgtttgcgg acaaccttgc agctgaaatt gaagaagata agattccagt cctcccaatc
    14641 tttacggccc ttgaaaaata cggaaagatc aagcatgaag aaatgtttga aatcttcaat
    14701 atgggtatcg gattggtcct ggcagtcagt ccagacaagg ttgacagtgt ctgtcaacta
    14761 gttgacgagg aagtttacac cattggtcgc atcatcgcca aggaagataa gagtgtggtc
    14821 atcaaatgaa acgaatagca gtgtttgcat caggcaacgg ctccaatttc caagtcatcg
    14881 cagaacagtt taaagtagct tttgtctttt cagaccgcag aaatgcctat gtcttggaac
    14941 gagctgaaaa actaggtgta ccaacctttg cttttgaact aaaagagttt tcagataagc
    15001 agacctacga agaagccatt atccaactat tagaccagca ccagattgac ttggtggtct
    15061 tggcaggcta tatgaaaatt gtgggtccga ccttgttagc tcaatatgaa ggtcgtatca
    15121 tcaatatcca cccggcttat ttgcctgaat ttccaggagc tcacgggatt gaagacgctt
    15181 ggaatgcagg tgtggcagaa agtggtgtga cagttcactg ggttgacagc ggtgttgaca
    15241 caggacaaat catcaaacaa gtccgagtgc cccgactggc cgatgatacc ctggaaacct
    15301 ttgaagccag aatacatgaa gcggagtacc aactctatcc agcggttttg gaggagttgg
    15361 gggcagtgaa aaaataagga gaaatcatga caaaacgtgc gttaattagc gtatcagata
    15421 aaaatggtat tgtagaattt gcccaagaat tgaccaagtt tggttgggaa attatctcga
    15481 ccggtggtac caaggttgcc ttggatcagg ctggtgtaac taccatcgcc attgacgatg
    15541 tgactggttt tcctgagatg atggacggcc gtgttaagac gcttcatccg aaaatacacg
    15601 gtggtttgtt agcacgtcgg gatttggaca gccacctgca agcagctaac gaccatgaaa
    15661 taggcttgat tgacttggta gtggttaacc tttacccctt caaagagact attttacgtc
    15721 cagatgtgac ctatgatttg gcggtggaga acatcgacat cggcggaccg tccatgcttc
    15781 gttctgcagc caaaaaccac gccagcgtga ccgttgtggt ggatccggca gattatccga
    15841 cggttttagg ggaaattgca gagcaggggg aaacgagcta tgcaacacgt cagcgattgg
    15901 ctgcaaaggt attccgtcat accgcagctt atgatgccct aatagcagat tattttacca
    15961 agcaagtagg cgaagataag cctgaaaaat taaccattac ttatgacctc aatcagccta
    16021 tgcgttacgg tgaaaatccc cagcaaaatg cggatttcta ccaaaacgcc cttccgacag
    16081 cctattctat tgcagctgct aagcagttaa atggtaagga gttgtctttc aacaacattc
    16141 gtgatgcgga tgcggccatt cggattatcc gtgattttaa ggaccgtcca actgttgtgg
    16201 ctctcaaaca catgaaccct tgtggtatcg ggcaggcaga aaccattgaa caggcttggg
    16261 attatgctta tgaggctgac ccagtatcga ttttcggagg catcgtcgtg ctgaacaggg
    16321 aagtggatgc tgcgacggct gaaaagatgc acccgatttt cttagaaatc atcatcgcac
    16381 cgggctactc ggcagaagcg ctagctattt tgaccaataa aaagaaaaat cttcggattt
    16441 tggagttggc ctttgacgca caggatgcaa gcgaagtgga aaaagagttc acaggcgttg
    16501 tcggcggact cttggtgcag gatcaggacg tggtggtgga aagcccagtg gactggcagg
    16561 tggtgaccga gcgtcaaccg tccgagcaag agtgggcagc catggagttc gcttggaagt
    16621 cctctaaata tgtcaagtcc aacggcatca tcatcaccaa tgacaagatg actttgggcg
    16681 tggggccggg gcaaaccaac cgtgtagcgt ccgtccgtat cgctatcgag caagccaagg
    16741 accgtttgga gggagccgtt ttggcgtcgg acgccttctt cccatttgct gataacgtgg
    16801 aagaaattgc agctgcaggt atcaaggcta tcatccagcc aggtggttct gtccgtgacc
    16861 aagactccat tgacatggcc aacaagtacg gcttgaccat ggtctttaca ggagtcagac
    16921 attttagaca ttgagaattt tggataagta tagcacccca gctggagaag ttggggtgga
    16981 ttttggtata atagttccat gactttaaaa ccttttacga aatggacagg tgggaagcgg
    17041 aagctattaa ctcagctaca tgaacatcta ccatttgaat acaatagata ttttgaaccg
    17101 tttgttggag gaggggcatt attctttgat ttagctcccg aaaatgccgt tataaatgat
    17161 tttaatgagg aattgataaa tacttattta cagatacggg ataatccaga agcattgctt
    17221 gatttattac atatccatca agagaacaat tccaaggaat attatttgga tgttcgttcg
    17281 gttgacagag atggtagaat agaaactatg tccgatgtgg agcgagcagc acgcatacta
    17341 tatatgctac gagtagattt taatggtcta tatagggtga actctaaaaa tcagtttaat
    17401 gtcccttacg gcaggtataa aaatcca
//
LOCUS       DBNFEG010000024        17359 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.024, whole genome shotgun sequence.
ACCESSION   DBNFEG010000024 DBNFEG010000000
VERSION     DBNFEG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 17359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17359
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.024"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(73..1293)
                     /gene="pepT"
                     /locus_tag="AC1PNG_000569"
     CDS             complement(73..1293)
                     /gene="pepT"
                     /locus_tag="AC1PNG_000569"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HIP6701092.1"
                     /translation="MKYPTLLDRFLVYVKENTRSDENSTTTPSTQNQVEFAQNILLPE
                     MKRIGLQNVHYLPNGFAVGTLPANDPSLTRKIGFIAHMDTADFNAEGVNPQIIENYDG
                     NPIALGTSGYELHPKDFPQLANYHGQTLITTDGTTLLGSDDKSGIAEIMTAIEFLVQN
                     PDIKHCEIRVGFGPDEEIGVGADKFDVEDFYVDFAYTMDGGPLGELQYETFSAAGAKI
                     DFLGRNVHPGSAKDQMINAFQMAIDFHNALPEADRPEKTEGYEGFFHLMNMEGSVDTA
                     STTYIIRDFEEEDFLARKQLMLDIAEKMNAKFDTPRVIVNLHDQYYNMKKIIEKDMTP
                     INIAKDVMENLGIKPLIEPVRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLETM
                     EKAVDVILGIVAYK"
     gene            complement(1303..2118)
                     /locus_tag="AC1PNG_000570"
     CDS             complement(1303..2118)
                     /locus_tag="AC1PNG_000570"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIP6701093.1"
                     /translation="MIKLIALDMDGTLLNEKKELMQPQIDAIHQAVEAGVKIVLCTGR
                     PLAGVKPFVEQLGFDTEEEFIIVNNGCSTHSTKDWSLIDWEELSISDIDYLSTFIEND
                     DVQISLFDEEDYFVLAEKANARVNLDAGLVGMTPQPIVLKEAQSGQHRFFEAMFVGEK
                     EHIDAFENQHNPVLSQHYSTVRSQDYLLEILPNGASKASGLKKLADRLGILPEEIMAM
                     GDANNDLEMIEFAGLGIAMGNANEQVKAIAQAITDTNENNGVAKAIEKHILNK"
     gene            2500..2970
                     /locus_tag="AC1PNG_000571"
     CDS             2500..2970
                     /locus_tag="AC1PNG_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIP6701094.1"
                     /translation="MKISVKKEAGHYLLSICYSVSASLTEAWDLLATDSGFAHWFPQL
                     NIVGNTLVFEMEGFREVMDLLEYRKNEKIAYRWDSATVSFTLSQLENQTLITFEERIP
                     EDFGNEFANAQKDMTGWLVPNECIKKFLEGQEPPVCQPLQEKWRTFLELELEGL"
     gene            2988..3245
                     /locus_tag="AC1PNG_000572"
     CDS             2988..3245
                     /locus_tag="AC1PNG_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701095.1"
                     /translation="MKKWWEQLKEEYYETRFDSNEDWMEWFIKRKMTWGWRWPTAITI
                     FVLWALSLPDARNVVRFFYFLLTLGVATVLHKLISYLKRRK"
     gene            3252..4505
                     /locus_tag="AC1PNG_000573"
     CDS             3252..4505
                     /locus_tag="AC1PNG_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HIP6701096.1"
                     /translation="MHQITCPHCGTAFQVNETEYSQLLAQVRGAEFDKEIHDRLERER
                     ELLSQKAENDLQARLSDKDKEILELTAKLDKFASQTELEISSAISQKDQEIQELKAQL
                     GQIGLAKDLELQQAVAQVEKERDAAQNALVLQEQKQELALATTRQEYEVRLKAADEQV
                     EFYKNFKAQQSTKAIGESLEQFAEAEFNKVRSYAFPHAKFAKDNEVSASGSKGDFIFR
                     DFDESGLEFISIMFEMKNEADTTKTKHKNADFFKELDKDRREKKCEYAVLVSMLEADN
                     DYYNTGIVDVSHEYEKMYVIRPQFFIQLIGILRNAALNSLQYQQELALVKEQNIDITH
                     FEEDLEIFKNAFAKNYQSASNNFQKAIDEIDKAIKRMEAVKAALTTSENQLRLANNKL
                     DDVSVKKLTRNNPTMKAKFEALREE"
     gene            4507..5001
                     /locus_tag="AC1PNG_000574"
     CDS             4507..5001
                     /locus_tag="AC1PNG_000574"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6701097.1"
                     /translation="MIVPLQFEHILQLDEAFQKQGWPSSKEILTNYLREQEKGERIVL
                     VAEETGVCKGYITLIKQVKEGPFYQSGIPEIADFNVFEDFQNQGIGWQLLDAICQLAS
                     EFTDIVGIGVGLNANYGKAQRLYVKHGFIPDGSGVWYRGRSLPVGAKAYNDDELALYF
                     TKKL"
     gene            5014..5244
                     /locus_tag="AC1PNG_000575"
     CDS             5014..5244
                     /locus_tag="AC1PNG_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HIP6701098.1"
                     /translation="MNTIDLNLPVAEIINQHPEVKDILVELGFKPLANPAMLNTVGKV
                     TNLKTGSKMTKIPLDRIQQVLECNGYEVIGGE"
     gene            5244..6569
                     /locus_tag="AC1PNG_000576"
     CDS             5244..6569
                     /locus_tag="AC1PNG_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HIP6701099.1"
                     /translation="MTEQRIDILKNILLDLHNGASPESVQDQFNQHFTGVSALEISMM
                     EHELMSSDTGITFEDVMNLCNIHANLFKGAIADVEVADMDQEGHPVYVFKQENLALRA
                     ALLRIRRIIDQHEQTEDAELKDQLLQGLTRQFGLLGQFENHYTRKEKVFFPIMERYGH
                     DAPPKVMWGVDDEIRDLFKTARKALEGGDLAATRAHFENFAKEFEEMIFKEEAILLMI
                     LLEIFTQDDWLQVAADSDAYGYAIVKPTAKWVPHRELFGEIAEQEVDSISPQPASPNQ
                     QIIDTPEGQFTITFTPKEKSETVQDRTSPQTFGNGYLSVEQANLILNHLPLELTFVNK
                     DDIFQYYNDSHPVEDMIFKRTPSQIGRHVELCHPPKIVDKVKKIFELLRTGQKDQVTM
                     WFKSESMGKFVYVVYKAVRDDQGEFQGVLEYVQDIQPFFEIDSDFHREI"
     gene            6600..6809
                     /locus_tag="AC1PNG_000577"
     CDS             6600..6809
                     /locus_tag="AC1PNG_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701100.1"
                     /translation="MDWPTIKIGDEGYYDAPLIFSYGYDEEAEEGIDEAVTFDAVPSE
                     TMRRIHKELENLIDTTFGSYRFYDD"
     gene            6859..7737
                     /gene="truB"
                     /locus_tag="AC1PNG_000578"
     CDS             6859..7737
                     /gene="truB"
                     /locus_tag="AC1PNG_000578"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIP6701101.1"
                     /translation="MISGIINVKKEAGMTSHDVVFKLRKILQEKKIGHGGTLDPDVTG
                     VLPIAVGKATRMIEYMQEEGKIYEGEITLGFSTTTEDSSGDLVERTPVLDIAEQAVDE
                     AMTSFVGTITQIPPMYSAVKVNGRKLYEYARTGEEVERPQRQIEIYSFERTSPIELVD
                     DCARFTFRVRCGKGTYVRTLSVDLGAKLGYASHMSKLERTGSAGMDLADALTLEEISS
                     LVAENDFSFLQPIERGIGDLPVVELKEEQVQDATFGRFVELNAEAERLAGFHDGQLIA
                     IFEKRDQLYKPSKVLV"
     gene            7772..8695
                     /locus_tag="AC1PNG_000579"
     CDS             7772..8695
                     /locus_tag="AC1PNG_000579"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIP6701102.1"
                     /translation="MNIRTIKHYSELNQEEPTVLVLGYFDGIHLGHKALLDRARQVAD
                     EKGLSVTVLTFPETPRLAFSRFSPELLLHLTSQDQRYHLLEQYGVDQLVLTDFTSEFA
                     SNTPQQFLERYIKGLNAQVLVAGFDYHFGNCRADVTDLTELFDGQVEIVSEVSLGGEK
                     VSSTRIRQAIQSGEVSLANQLLGYPFMTEGIVVHGDARGRTIGYPTANLAPFDRVHLP
                     SEGVYVADVEVDGKRYRAMTSVGKNVTFDGTEMRIEAHIFGFHRFIYGEKITIFWLEK
                     IRDMVKFDGIEGLMEQMKSDEKFALQWGKYA"
     gene            8688..9434
                     /locus_tag="AC1PNG_000580"
     CDS             8688..9434
                     /locus_tag="AC1PNG_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIP6701103.1"
                     /translation="MLKRLIRHPFYEKAMTYIAVVYVFLIVLELMDASIANSSGYRVL
                     DLLLWFIFVCDYFAQLYYADDILEYVQGHILELIAIIPFDSFFSFLRLGRLARLFRLV
                     KVTRIFALSNRFWDTINKLLHTNSLSKVLMLNISAVLVSSVLLSVIEGKSFFDALWWS
                     IVTMTTVGYGDIVPQDTISKIIAILLMLVGICTFGMVTSTITRFFSETERETKLDILM
                     AKIDEQNETLLRLEKKIESLEKLGELQGRD"
     gene            9509..9910
                     /locus_tag="AC1PNG_000581"
     CDS             9509..9910
                     /locus_tag="AC1PNG_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HIP6701104.1"
                     /translation="MITLFLSPSCTSCRKARAWLTSHEVPFIEHNIMTSPLTAEELKN
                     ILALTENGTDDLISTRSKVFQKLDIDVDELSVKQLIGLIETYPSLLRRPIILDEKRMQ
                     IGFNEDEIRAFLPRKYRKQELKNATLRAEIG"
     gene            9925..10203
                     /locus_tag="AC1PNG_000582"
     CDS             9925..10203
                     /locus_tag="AC1PNG_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HIP6701105.1"
                     /translation="MNKNYSYPLDFSWSTEEISSVLSFLNQVEAAYEKGADAVAILAS
                     YKSFKDVVKSKGQERQIDRDFEAVSGYSTYRVVKAARDKGKGVIRFGN"
     gene            10193..10951
                     /locus_tag="AC1PNG_000583"
     CDS             10193..10951
                     /locus_tag="AC1PNG_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIP6701106.1"
                     /translation="METKYHFAQTIVLEAGNFLRQHLHDDLQIEEKGDFTDLVTHLDK
                     QVQEMLTQQIQSRYQGDGILGEEGGDVSSIHEGNVWVIDPIDGTTNFIAQKTDFAIMI
                     AYFENGVGKFGIIYDVMRDKLFHGGDSFPVYCNQERLKKPELRPLRQSLIGINAKLYA
                     GNAHGVAELANQSLGTRSVGSAGIGFTHVLEGRLFAYASYLCPWDYAAASIIGSSLGL
                     VMLSFEGPSLTYDKREFVMLVSESHLKEVKGYIF"
     gene            10951..12252
                     /locus_tag="AC1PNG_000584"
     CDS             10951..12252
                     /locus_tag="AC1PNG_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HIP6701107.1"
                     /translation="MQLPQDFIDKYNHLLGSEAQAFFESFEQDPISGFRTNPLKESQL
                     DFEQPIPNMTWSYYGKVSGKSPEHVTGLVYSQEPAAQVVGQIAAPEKGMKVLDLAAAP
                     GGKSTHLLSYLENTGLLVSNEISNKRAKILAENIERFGARNVVVTNESADRLAKVFPG
                     YFDMIVLDAPCSGEGMFRKDPDAIQYWSKHYPAQCASLQREILESALNMLAPGGQLIY
                     STCTWSPEENEEVVTWLLEQYDLELIDIPKINGMVEGVGYPEVARMYPHHFAGEGQFV
                     AKFRFLGEVSQKKLKPGKSNLNREQQALWKEFEQKHLRVQLDGLLQVFGDNLYLLPHG
                     LPDLSKVKIARNGLHLGTYKKKRFEPSFALGLALHSSEVENRVEISLEEFSSYTAGHT
                     IAVSKDLANGWYQVAVQGNGLGFAKIVSGTLKNGFPKGLRF"
     gene            12389..13258
                     /locus_tag="AC1PNG_000585"
     CDS             12389..13258
                     /locus_tag="AC1PNG_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HIP6701108.1"
                     /translation="MKKKQKLGIAALFLLSSMALSGCSSWIDRGESITAVGSTALQPL
                     VEAAADEFGSVNIGKSVNVQGGGSGTGLSQVQSGAVQIGNSDVFAEEKDGIDASKLVD
                     HQVAVAGLAVIVNEQVSVKDITTEDLRKIFTGEITNWKELGGKDLEISIINRAASSGS
                     RATFDSVIMDGQTATQSQEQDSNGMVKSIVAQTPGAISYLSFAYLDDSVKTIKLNGFE
                     PTAENVATNDWPIWSYEHMYTMGKATDLTEEFLDYMMSDEVQNGIVESMGYISINDMK
                     VVKSADGTVTEKE"
     gene            13261..14178
                     /gene="pstC"
                     /locus_tag="AC1PNG_000586"
     CDS             13261..14178
                     /gene="pstC"
                     /locus_tag="AC1PNG_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIP6701109.1"
                     /translation="MKNEQLAKELSSPSKNSRLEKFGKVITFLCLSLIVFIVAMILIF
                     VAQRGLSTFFVDGVSITDFLFGSKWEPSSKIFGALPMISGSFIVTILSAVVATPIAIG
                     AAVFMTEISPKRGAKILQPVIELLVGIPSVVYGFIGLQVVVPFVRSIFGGTGFGILSG
                     VFVLFVMILPTVTFMTVDSLRAVPRHYREASLAMGATRWQTIWRVILNAAKPGIFTAV
                     IFGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSVLTMGIGNTVMGTVQNNVLWS
                     LALVLLLMSLIFNMIMKFITREGKKNYAR"
     gene            14168..15055
                     /gene="pstA"
                     /locus_tag="AC1PNG_000587"
     CDS             14168..15055
                     /gene="pstA"
                     /locus_tag="AC1PNG_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIP6701110.1"
                     /translation="MHAKKFDKLATGILYSIAGVIVVILTSLILYILLRGLPHVSWDF
                     LTSKSSSYKAGGGIGIQLYNSFFLLVVTLLISVPLSTGAGIYLAEYAKKGPLTNFIRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSILSGALALTVFNLPQMTRNVEDSLRHVH
                     HTQREAGLALGLSRWETVLHVVIPEALPGIVTGIVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNPLSVTSPISIFRQSETLAVHIWKVNSEGTIPDATQVSAGSAAILLVFILIF
                     NLSARYIGKKLHAKLTSAA"
     gene            15080..15883
                     /gene="pstB"
                     /locus_tag="AC1PNG_000588"
     CDS             15080..15883
                     /gene="pstB"
                     /locus_tag="AC1PNG_000588"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIP6701111.1"
                     /translation="MADYNWNERHIITFSEENIALSTKDVHVYYGKNEAIKGIDMQFE
                     KNKITALIGPSGCGKSTYLRSLNRMNDTIDIAKVTGQILYEGIDINRPDMNVYEIRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAISVKPQILLMDEPASALDPIATMQLEETMFELKKDYTIIIV
                     THNMQQAARASDYTAFFYLGDLIEYDKTANVFQNAKLKSTSDYVSGHFG"
     gene            15895..16653
                     /gene="pstB"
                     /locus_tag="AC1PNG_000589"
     CDS             15895..16653
                     /gene="pstB"
                     /locus_tag="AC1PNG_000589"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIP6701112.1"
                     /translation="MTSPILQVKDLSVYYNKKKALNNVSIDFYPNEITALIGPSGSGK
                     STLLRSINRMGDLNPEVTLTGAIDYNGKNIYSPRTDTVDLRKEIGMVFQQPNPFPMSI
                     YENVVYGLRINGIKDKQVLDEAVERSLIGASIWNEVKDRLHDSAVGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEDTLYGLKDKYTMVLVTRSMQQASRISDRTG
                     FFLDGDLIEYNLTKEMFLNPANKETEDYITGKFG"
     gene            16682..17338
                     /gene="phoU"
                     /locus_tag="AC1PNG_000590"
     CDS             16682..17338
                     /gene="phoU"
                     /locus_tag="AC1PNG_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIP6701113.1"
                     /translation="MLRAQFEEELGKLHNQFYAMGNEVLAQINNAVRAFTTHDRELAK
                     EVIEADKKINAFEVKLEKKSLEIIALQQPVSTDLRRVITVLKATSDLERMGDHAVSVA
                     KAAIRMKGEVRIPVVEEEIKKMGREVRQLVEETLELYLGDADMERAYEVAARDEVVNE
                     FYSTIQELTTEEIKKNPESLVAGRDYYQVLTYLERVGDYAKNICEWVVYLRTGDITEL
                     "
BASE COUNT         5087 a         3191 c         4056 g         5025 t
ORIGIN      
        1 acaatataaa attgcatttt tttaaaattt tttcagaaaa aaacgacttt ctattgaaag
       61 ccgtttgaaa ttttatttgt aagcgacgat tccaaggatg acatcaacag ctttttccat
      121 ggtttccaag ctgacgaact cgaagcgacc gtgcatgttt tcgccacctg cgaagatatt
      181 tggtgttgga atgcccatga aggaaatctt agagccgtct gttcctccgc gaacaggctc
      241 aatcagtggc ttgatgccta gattttccat gacgtctttg gcaatgttga ttggagtcat
      301 atctttttcg atgatttttt tcatgttgta gtattggtcg tgcaggttga caatgacgcg
      361 gggtgtgtca aacttggcat tcattttctc tgcaatatcc agcatgagct gtttgcgggc
      421 aaggaaatct tcttcctcga agtcacggat gatgtaggtg gtagaagcag tatcaacact
      481 accttccata ttcatcaaat ggaagaaacc ttcatagcct tctgttttct cagggcggtc
      541 tgcttctgga agggcattgt ggaaatcaat agccatttgg aaggcattga tcatctggtc
      601 cttagcagat cctgggtgga cattgcgtcc taggaaatca atcttggcac cagctgctga
      661 gaaggtttcg tactggagtt cgcccagcgg tccaccgtcc atggtgtagg caaagtccac
      721 atagaagtct tccacatcaa acttgtctgc tccgacaccg atttcttcgt caggaccaaa
      781 gccgacacgg atttcacagt gtttgatgtc aggattttgg actaggaatt caatcgcagt
      841 catgatttct gcaattcctg acttgtcatc agagcctagc aaggtagtac cgtcagtggt
      901 aatcaaggtt tgaccgtggt agttggcaag ttgtgggaag tcttttggat gcaattcata
      961 gccagaagtt cccaaggcaa ttgggttacc gtcgtagttt tcgatgatct gcggattgac
     1021 accttcagcg ttgaaatcag ccgtatccat gtgggcgatg aagccaatct tacgtgtcaa
     1081 acttgggtcg ttggcaggca gtgttcccac agcaaagccg tttggtaggt agtggacatt
     1141 ctgtaagccg atgcgtttca tttcaggcag aaggatattc tgtgcaaatt cgacttggtt
     1201 ttgggtagaa ggtgtggtgg tcgaattttc atctgagcga gtattttctt tgacatagac
     1261 taaaaaacgg tctaagagag ttgggtattt catagtatct ccttatttgt tcaaaatgtg
     1321 tttttcaatg gctttggcaa cgccgttgtt ttcattggtg tctgtgatgg cctgggcgat
     1381 agccttgacc tgctcgttgg cgttgcccat ggcaatacca agtccagcaa attcaatcat
     1441 ttccaggtcg ttgttggcat ctcccatagc catgatttct tctgggagga taccgaggcg
     1501 gtctgccagt tttttcaaac cagaggcctt gcttgcaccg tttggtaaaa tttccaagag
     1561 atagtcctgt gaacgaacgg tggaatagtg ttggctgagt acaggattgt gttgattttc
     1621 aaaggcatca atatgctctt tttcaccgac gaacatggct tcaaagaagc gatgctgacc
     1681 tgactgggct tcttttaaaa caattggttg aggagtcatt cctactaaac ctgcgtctaa
     1741 attaacgcgt gcattggctt tttctgcaag aacgaagtag tcttcctcat caaaaagaga
     1801 gatttgcaca tcatcatttt caataaaagt ggaaagataa tcaatatcag agatagatag
     1861 ttcttcccag tcaatcaggc tccaatcctt tgtcgaatgc gttgaacaac cgttattgac
     1921 aatgataaat tcttcctcag tgtcaaagcc gagctgctct acgaaaggtt taacaccagc
     1981 caagggccga ccagtacaaa gaacaatctt gacgcccgct tcaacagcct gatgaatggc
     2041 atcaatctgt ggctgcatca gctctttttt ctcgtttaaa agggttccgt ccatatcaag
     2101 agcgattagt ttaatcatat aaacctcctt gttttcaata catctagtat agcacaaaat
     2161 gagaaaggaa tgattgaaat actaaactct gttctatatg ttaaaataaa ttaggtgacg
     2221 aatgaaatat agaagtgttg caaaaatgac aatgtccatt atgtgttaag aaagtctaac
     2281 attgctaggg gatacaaaac atggagtatg agccaggtaa acaaaggtaa ggactgtcta
     2341 tgaaagatgt tataaaaaat aattcagatt tcaagatacc gtttttcgat cctctggaat
     2401 gaatagcatt catgcttaaa gaatagagac aatggaaata tcggttatag accggtgttt
     2461 tttggtataa ttaatagaag gatagaatag gagcatgata tgaagattag tgtgaagaaa
     2521 gaggcggggc actacctgct cagcatttgc tattctgtgt cggctagttt gacagaagct
     2581 tgggacttgt tggcgacaga cagtggtttt gcccactggt ttccacagtt gaacattgta
     2641 ggaaatacgc tggtttttga aatggaagga ttccgtgaag ttatggattt actggaatat
     2701 cgaaaaaatg agaaaattgc ctacaggtgg gattcagcaa cagtttcatt tacactgtct
     2761 cagctagaaa atcagacctt aattactttt gaagaacgaa ttccagaaga ttttggtaat
     2821 gaatttgcga atgctcaaaa agacatgaca ggatggttgg tgccaaatga atgtatcaaa
     2881 aaattcttag aaggtcagga gccgcctgtg tgtcaaccct tgcaggaaaa gtggaggact
     2941 tttctggagc ttgaattaga aggtctgtga tctgaaagga ggctgttatg aaaaagtggt
     3001 gggaacaact gaaagaagaa tattatgaaa cacggtttga ttctaatgaa gattggatgg
     3061 aatggtttat caaacggaag atgacctggg gctggagatg gccgactgct ataacgattt
     3121 ttgtcctttg ggctctatca cttccagatg ctagaaatgt ggttcgtttc ttttattttt
     3181 tattgacgct gggagtagct acggttttac ataaattaat atcttatttg aaaagaagaa
     3241 agtgagagta tatgcatcaa attacttgcc cccattgtgg gacggccttt caggtcaatg
     3301 aaacagaata tagtcagctt ttagcccagg tgcgtggtgc ggagtttgac aaggaaatcc
     3361 acgaccgttt ggagcgggaa cgggaattat taagccagaa ggctgaaaat gatttgcagg
     3421 ctagattgag tgataaggat aaggagattc tggaactgac cgcaaaactt gataaatttg
     3481 ctagtcagac ggaactggaa atcagctctg ctatttccca aaaagaccaa gaaatccaag
     3541 agcttaaggc tcagttgggg caaatcggtc ttgccaagga cttggagttg cagcaggcgg
     3601 tagctcaggt ggaaaaagag cgggatgcgg cacaaaatgc tttggttttg caggaacaaa
     3661 agcaagagtt agccctagcg accactcgtc aggagtacga agtacggctc aaggcggcgg
     3721 atgagcaggt ggaattttac aaaaatttca aggcccagca gtctactaag gcaattggag
     3781 agagcttgga gcaatttgct gaggcggagt ttaacaaggt tcgttcctat gcttttccac
     3841 atgccaaatt tgcgaaggac aacgaagtat cagcgtccgg ttcaaaagga gacttcatct
     3901 tccgtgattt tgacgagtcg ggcttggaat ttatttccat catgttcgaa atgaaaaacg
     3961 aggcagatac gaccaagacc aagcataaaa acgctgattt ctttaaggag ttggataagg
     4021 accgtcggga gaaaaaatgc gaatatgctg tattagtcag catgctagag gcggacaatg
     4081 actattacaa tacagggatt gtggatgtta gtcatgaata tgagaagatg tatgttattc
     4141 gaccacagtt ctttatccaa ttaatcggaa ttttacgaaa tgctgcgctc aatagtctgc
     4201 aatatcagca ggagttagcc ttggttaagg agcagaatat tgatattact cattttgaag
     4261 aagatttgga aattttcaag aatgcttttg ccaaaaacta tcaatcggcc agcaacaatt
     4321 tccaaaaagc tattgatgaa atcgataagg caatcaagcg aatggaggct gtcaaggcag
     4381 ccctgaccac cagcgaaaac cagcttcgcc tagccaataa taaactagac gatgtctccg
     4441 tcaaaaaact aacccgcaat aacccaacca tgaaagccaa gtttgaggcg ttgagggagg
     4501 agtaggatga ttgttccgtt acaatttgag catatacttc aacttgatga ggccttccag
     4561 aagcaaggtt ggccctcaag caaagagatt ttaaccaact atttaaggga acaagaaaag
     4621 ggagaacgta tcgtcctagt tgctgaagaa acgggagttt gtaaaggtta tatcaccttg
     4681 attaagcaag tgaaggaggg gccgttttac caatctggaa tacctgaaat tgctgatttc
     4741 aatgtttttg aagactttca aaatcagggg ataggttggc aattattaga tgctatctgt
     4801 cagcttgcct cagaattcac ggatatagta ggtattggag ttggtttgaa tgcaaattat
     4861 ggaaaggctc aacggctcta tgtaaaacat ggtttcatac cagacggaag cggtgtctgg
     4921 taccgagggc gctctttgcc agtaggtgcc aaagcctata acgatgatga attggccttg
     4981 tattttacaa aaaaattata gaggaaaagt aaaatgaaca ccatcgacct aaatctgcct
     5041 gttgcagaga ttatcaacca gcacccagaa gtcaaggata ttctggtgga gcttggtttc
     5101 aagcctttgg caaatccagc tatgctcaac acagttggta aggtgaccaa tctgaagaca
     5161 ggctccaaga tgaccaagat tccgttagac cgtattcaac aggtcttgga atgcaacggc
     5221 tatgaggtaa taggaggaga ataatgacgg aacaacggat tgacattttg aagaatattc
     5281 tcttggacct gcacaatggt gccagtccag agtctgttca ggaccagttt aatcagcatt
     5341 tcacgggagt ttctgccttg gaaatctcca tgatggagca tgaactcatg tccagcgaca
     5401 cgggtatcac ttttgaagac gtcatgaacc tttgcaatat ccacgccaac cttttcaaag
     5461 gagccattgc ggatgtggaa gtggcggata tggatcagga aggacacccc gtctatgtct
     5521 tcaagcagga aaatctagca ctgcgggcgg ctcttttacg catccgacgt atcattgacc
     5581 agcatgagca gacagaggat gcggagctga aagaccagct cctgcaaggt ttgacacgcc
     5641 agtttggcct cctcggtcaa tttgaaaatc actatacccg caaggaaaag gtcttctttc
     5701 cgattatgga gcggtacggt catgatgcac cgcccaaggt tatgtggggt gtggacgacg
     5761 agattcgcga tctcttcaag acggcccgca aggcacttga aggaggtgac ctagctgcga
     5821 cacgtgctca ctttgaaaac ttcgccaagg aatttgaaga gatgattttc aaggaggagg
     5881 ccattctcct catgattctg ctggagattt tcacccagga cgactggctc caagtggcag
     5941 cagacagcga tgcctacggc tacgccattg ttaagccgac agccaagtgg gtacctcatc
     6001 gggagctttt tggagaaata gcagagcagg aagtagatag catttctccg cagccagcta
     6061 gcccaaacca gcagattatc gacacgccag aaggccagtt taccattacc tttacaccca
     6121 aggaaaagtc agaaactgtt caggacagga ctagtccgca gaccttcggc aatggttatt
     6181 tatctgttga gcaagccaat cttatcctca atcatttacc gctggaatta acctttgtca
     6241 acaaggacga cattttccag tactacaatg acagccatcc tgttgaagac atgattttca
     6301 aacgaacacc aagccagatt ggacgccatg tggagctctg ccatcctccc aaaatagtgg
     6361 acaaggtcaa gaagattttt gaattgctcc gcacagggca aaaagaccag gtcaccatgt
     6421 ggttcaagtc cgagagcatg ggcaagtttg tctatgtggt ctataaggca gtgcgtgatg
     6481 accaaggaga gtttcaaggc gttttggagt atgttcagga tattcaacca ttttttgaga
     6541 ttgatagcga ttttcatcgg gaaatttagt ttagtcattt ttgaaaggaa attaagctgt
     6601 tggattggcc gaccataaaa attggtgacg aaggctatta tgatgcccca ttgatatttt
     6661 cttatggcta tgatgaagag gctgaggaag ggattgatga agcagtaaca ttcgatgcag
     6721 taccttctga aaccatgcga cgcattcata aggaactaga gaatttgata gatacaacat
     6781 tcggtagcta tcgtttctat gatgactgat tgatatcgga ccgcccacaa gagcggtcct
     6841 ttgtgttata atggaaaaat gatttcagga attattaacg taaaaaaaga ggcaggtatg
     6901 acctcgcatg atgttgtttt taagctccgc aagattttgc aggagaagaa gattggacat
     6961 ggtgggacct tggatcccga tgtgacaggc gttctgccca tcgcagtcgg taaggccacg
     7021 cgtatgattg agtacatgca ggaagaggga aagatttacg agggggagat taccctcggc
     7081 ttctcaacca cgaccgaaga ttcgtcgggc gacctcgtcg agcggacacc ggttttggac
     7141 atagcagagc aggcagttga tgaggctatg accagtttcg tcggtactat tacccagatt
     7201 ccgcctatgt actctgctgt caaagtaaac ggtcgcaaac tatacgaata cgcacggacg
     7261 ggtgaagaag ttgagcgtcc tcaacgccaa attgagattt acagttttga acggactagc
     7321 ccgattgaac tagtagatga ctgtgcacgt tttaccttcc gagttcgctg tggtaagggc
     7381 acctatgtcc gtaccctgtc tgttgaccta ggggctaagt tgggctatgc cagccatatg
     7441 tccaagttgg aacgaacagg atcagctgga atggacctag cggatgcctt gactttagaa
     7501 gaaatttctt cgctggtagc tgaaaatgat ttttcattcc tccaacctat tgaacgtggc
     7561 atcggggatt tacctgttgt agaattgaaa gaagagcagg tgcaagatgc tacatttggt
     7621 cgatttgtag aattgaatgc agaagcagag cgcttagcag gctttcatga tggtcagttg
     7681 attgctattt ttgaaaaacg cgaccagctg tataaaccga gcaaggtttt ggtctaagaa
     7741 atttctcaat aactgtgata aaatagaaaa catgaacatt agaacaatta aacattatag
     7801 tgaattgaat caagaggagc cgactgtttt ggtattaggc tattttgatg ggattcattt
     7861 ggggcataag gccctcttag accgtgctag acaagttgca gatgaaaaag gattgtcagt
     7921 gaccgtactg acttttccag agacccctcg tctggctttt tctcgcttta gcccagaact
     7981 tttactgcat ttgaccagtc aagatcagcg ttatcacttg ttggagcaat acggtgtaga
     8041 ccagctggtt ttgacagatt ttaccagtga gtttgcaagc aatactcctc agcagttttt
     8101 agaacgctac atcaaggggt tgaacgcaca agtcttagtg gcaggtttcg attatcattt
     8161 cggaaattgc agagcagatg tgacagactt gacagagtta tttgatggtc aggtagaaat
     8221 tgtaagtgaa gtaagcctgg gcggagagaa agtttcttca actcgcattc gtcaggctat
     8281 ccagtcaggg gaggtctctc tagctaatca acttttaggt tatccattta tgacggaggg
     8341 aattgttgtt catggagatg ccagagggcg cactattggt tatccaactg caaatctagc
     8401 tccttttgac cgtgtccatt taccatctga gggagtctac gtcgctgatg tcgaagtaga
     8461 tggaaaacgc tatcgagcta tgacaagcgt tgggaaaaat gtgacctttg atggaacgga
     8521 gatgcggata gaagcccata tttttgggtt tcaccgcttt atctacggag aaaaaataac
     8581 tattttttgg cttgaaaaaa ttcgagatat ggtcaaattt gatggaattg aaggattgat
     8641 ggagcagatg aaatctgatg agaaatttgc cttgcaatgg gggaaatatg cttaaacgat
     8701 tgattcgtca tcctttttat gagaaggcta tgacctacat agctgtagtc tatgtatttc
     8761 ttatcgtact tgaattaatg gatgctagta ttgccaattc tagtgggtat cgggtactag
     8821 acttgctctt atggtttatt tttgtttgcg attattttgc tcagctttat tatgcggatg
     8881 acatacttga atatgtacag ggacatatct tggaattaat tgccattatt ccatttgatt
     8941 cattcttcag ttttcttcgc ttgggaagac ttgcacgact cttcagattg gtcaaagtaa
     9001 cgagaatatt tgccctgtct aatcgattct gggatacaat caacaagctt ttgcatacca
     9061 acagtttatc caaagtgttg atgttaaata tctcagcagt gctagtttca agtgtcttat
     9121 tatcagtaat tgaaggaaaa tcattcttcg atgcactttg gtggtccatt gtaacgatga
     9181 caactgtagg gtatggagat attgtccccc aagatacgat ttctaaaatt atagccatcc
     9241 tcttgatgtt ggttggtatc tgtacttttg gtatggttac ctccacaata actcgttttt
     9301 tctctgaaac agagcgagaa acgaagttgg atatcttgat ggcaaaaatt gatgaacaaa
     9361 acgaaacatt attacgctta gagaaaaaaa tagaatcttt agaaaaatta ggtgaattac
     9421 agggaagaga ttagatttac tattgaaaac tcttttaagt tagagtataa taataagaag
     9481 aaaaatgttt ttagagggga tggaagacat gattacttta tttttgtcac cgagttgtac
     9541 cagttgtcgt aaagctcgtg cttggctgac aagtcacgaa gtccctttta ttgagcacaa
     9601 tattatgact agccctctaa ctgctgagga attaaagaac attttagcat tgacagaaaa
     9661 tggaacagat gacttgattt ctacccgttc aaaagttttt cagaaactag atattgatgt
     9721 tgatgagctt tctgtgaagc agcttattgg gttaatcgaa acctatccga gtctcttacg
     9781 gcgtccgatt attttggatg agaagcggat gcagattggt tttaacgaag atgaaattcg
     9841 tgcatttctt ccgcgcaagt atcgcaaaca agagctaaaa aatgctacat tgagagcaga
     9901 aataggataa gaaagataaa agatatgaac aaaaattatt catatccact tgatttttcg
     9961 tggagcacag aggagatttc ctccgtgctt tcttttttga atcaggtgga ggcagcttat
    10021 gaaaaaggag cagatgcggt agctatatta gctagttata aaagcttcaa agatgttgtt
    10081 aaaagtaagg gacaggaacg tcaaattgat cgtgactttg aagcagtttc tggttattca
    10141 acctatcgag ttgttaaagc tgctcgagat aaggggaaag gagttattcg ttttggaaac
    10201 taagtatcac tttgcccaaa cgattgtcct ggaggctgga aattttctcc gacagcattt
    10261 acatgatgat cttcagattg aagaaaaagg tgactttaca gacttggtaa cacaccttga
    10321 taaacaagtg caagaaatgt tgactcaaca gattcaatct cgctatcaag gtgatgggat
    10381 tttaggcgag gaaggtgggg acgtttcttc tattcatgag ggaaatgttt gggttattga
    10441 tccaattgat ggcaccacaa attttattgc tcagaaaact gattttgcca ttatgattgc
    10501 ctattttgaa aatggagtag gaaagtttgg tatcatttat gatgtcatgc gagacaaact
    10561 tttccatgga ggagattcat tccctgttta ttgcaatcaa gaacgtttaa agaaaccaga
    10621 gctgcgccct cttcgccaga gtttgatagg aatcaatgca aagttgtacg caggcaatgc
    10681 acatggagtg gcagaattag caaatcaaag tttaggtaca agatctgtag gaagcgctgg
    10741 gattggtttc acccatgttc tggaaggaag attatttgcc tatgcttctt atctgtgtcc
    10801 atgggactac gcagcagcca gtattattgg ttcctcttta ggcttagtca tgttaagttt
    10861 tgagggtccg agtctgacct atgataagcg agagtttgtc atgctagtat cagaatccca
    10921 tcttaaggaa gtgaaggggt atatattcta atgcaattac cacaggattt tatagataaa
    10981 tataatcatt tgcttggtag tgaagcccaa gcattttttg agagttttga acaagaccca
    11041 atctcaggat ttcgcacaaa tcctcttaaa gaaagccaat tggactttga gcaaccaatc
    11101 cctaatatga cttggtcata ctatggtaag gtctcaggaa agtctccaga acatgtaact
    11161 ggcttagtct actcacaaga gccagctgca caggttgtag gtcagattgc agccccggaa
    11221 aaagggatga aagtattgga cttggcagcg gcgccaggag gaaagtctac ccacttactt
    11281 tcatacttag aaaatacggg gctcttggtt agtaatgaaa tttctaacaa acgagcaaaa
    11341 atattggctg aaaatattga gcgtttcggg gcgagaaatg tagtcgtgac caatgaatct
    11401 gccgatcgtt tggccaaggt ctttccagga tatttcgata tgattgtcct agacgctccc
    11461 tgttcaggtg aagggatgtt tcgaaaagac cccgatgcga ttcaatattg gtccaaacat
    11521 tatccagcac aatgtgcaag cttacagcgt gaaattttag aatcggcctt gaatatgttg
    11581 gctccaggtg gtcaattgat ttattctact tgcacttggt caccagagga aaatgaagaa
    11641 gtagtgacgt ggctcttaga acagtatgat ttggaactaa tagacattcc taaaatcaat
    11701 ggaatggtag aaggagtcgg ttatccagaa gttgctcgta tgtatcccca ccattttgca
    11761 ggagaaggtc agttcgttgc caaatttaga tttctaggag aagtcagtca gaaaaaatta
    11821 aaaccaggaa aatccaattt gaatagagaa cagcaagctc tctggaaaga atttgaacag
    11881 aagcatttaa gggttcaatt ggatggttta ctgcaggttt ttggcgacaa tctctaccta
    11941 ctgccacacg gccttcctga tttgtcaaaa gtaaagattg ctcgaaatgg tcttcacttg
    12001 gggacgtata agaaaaaaag atttgaacca agttttgctt taggtctagc actccatagt
    12061 tcggaggttg aaaatcgagt ggaaatcagc ttagaagaat tctcgtccta tactgcagga
    12121 cataccatag ctgtgtccaa ggatttagct aatggctggt atcaggtagc agtccagggg
    12181 aacggtcttg gtttcgcaaa aattgtatct ggaacgctta aaaacggctt tccaaaaggg
    12241 ttgagatttt aaaataaatc agcaaatttt tttgcaactt gtctttttat atgatatagt
    12301 ggatgagtgg aaatatcttg taaattctat gtaaaataca gagttaattt taggagattc
    12361 cactgaaaaa tgtaaaggaa aaaacgaaat gaaaaaaaag cagaagcttg gaattgcagc
    12421 tttgtttctt ttgagtagta tggctctttc tggttgttct tcttggatcg atcgaggaga
    12481 gtctattaca gccgtaggtt cgacggcgct tcaaccactt gttgaggcag cagcagatga
    12541 atttggtagt gtcaatatcg gtaaatccgt caacgttcag ggtggtggat caggtactgg
    12601 cttgtcacaa gttcagtctg gagctgttca gattggtaac tctgacgtat ttgctgagga
    12661 aaaagatggt attgatgcaa gtaaacttgt tgaccatcaa gtagcagtag ctggtttggc
    12721 cgttattgta aatgagcagg tatcggttaa agatattact acagaagatc tgcgtaaaat
    12781 ttttactggt gagattacca actggaaaga actaggtggt aaggatttgg aaatttcaat
    12841 cattaaccgt gcagctagtt caggttcacg tgcgaccttt gatagtgtta tcatggatgg
    12901 acaaaccgca actcagagtc aagaacaaga ttctaacggt atggtaaaat ctattgttgc
    12961 tcagacacca ggtgcaattt cctacctatc atttgcttat ctagatgatt ccgttaaaac
    13021 aattaagtta aacggttttg aaccaactgc tgaaaatgta gcaacaaatg attggccaat
    13081 ttggtcctat gagcatatgt atacgatggg aaaagcgact gatttgacag aagaattttt
    13141 agattacatg atgtcagatg aagttcaaaa tggtatcgtt gaaagtatgg gatacatttc
    13201 tatcaacgat atgaaagttg tgaaaagtgc tgatggcacg gtgactgaga aggagtaagc
    13261 atgaaaaacg aacaactagc aaaagagtta tcgtctccat ctaaaaattc tcgattagag
    13321 aagtttggta aggtaattac attcctttgt ctatcattga ttgtctttat tgttgcaatg
    13381 attttgattt tcgttgcgca gcgtggatta tcgactttct ttgttgacgg tgtgagcatc
    13441 actgatttct tatttggaag caagtgggaa ccaagctcga aaatatttgg tgccctgcca
    13501 atgatttcag gttctttcat tgttacgatt ctgtcagctg ttgttgcgac ccctatcgca
    13561 atcggtgcag cagtctttat gacagaaata tcaccaaaac gtggtgcaaa aatcttacaa
    13621 cctgttatcg aacttttggt tggtattcca tcggttgtat atggtttcat tggtttgcaa
    13681 gtagttgtac catttgtacg ttcaatcttc ggtggaacag gtttcggtat cttgtcgggt
    13741 gtatttgtac ttttcgttat gattttgcca acagtaacct ttatgacggt tgatagtttg
    13801 cgtgcagtac ctcgtcacta ccgcgaagca agtttggcta tgggagctac ccgctggcag
    13861 acgatttggc gtgttatttt gaacgcggct aagccaggta tttttacggc cgttatcttt
    13921 ggtatggcgc gtgcctttgg tgaagccttg gcgattcaga tggtagttgg taactcagcg
    13981 gttatcccga cttccttgac aacgccagca gcgactttga cttctgtttt gacaatgggt
    14041 atcggtaata cggttatggg aactgttcag aacaacgtcc tatggtcctt ggccctagtc
    14101 ttgctcttga tgagccttat ctttaacatg attatgaaat ttattacgag agagggtaag
    14161 aaaaactatg cacgctaaaa aatttgataa attggcgact ggtattcttt actcaatcgc
    14221 tggtgtgatt gtcgttatct taacgtcact gattctttac atcttgctac gtggtctgcc
    14281 acatgtaagc tgggattttt tgacaagtaa atcttcatct tacaaagctg gcggtggtat
    14341 tggaattcag ctctacaact cattcttcct attggttgta acgctcttaa tttctgttcc
    14401 tttgtcaacc ggtgcaggta tttatttggc tgaatatgct aaaaaaggtc cattgactaa
    14461 ctttatccgt acctgtattg agattttgtc atccctaccg tccgttgtcg ttggtttgtt
    14521 tggttacttg attttcgttg ttcagtttga gtacggtttt tctatcttat caggtgcctt
    14581 ggctcttaca gtcttcaact taccacagat gacgcgtaac gtagaggaca gccttcgtca
    14641 cgttcaccat acacaacgtg aagcaggatt ggctcttggt ctatcacgtt gggaaactgt
    14701 tctccatgtt gttattccag aagcacttcc agggattgta acaggtatcg tcttggcatc
    14761 tggtcgtatc ttcggtgagg cagcagctct tatctacacg gctggtcagt cagctccagc
    14821 tcttgactgg tcaaactgga atccattgag tgttactagt ccgatttcta tcttccgtca
    14881 atcagaaacc cttgcggttc atatctggaa ggtaaacagc gaaggtacaa tcccagatgc
    14941 aacccaagtt tctgctggtt cagcagctat tctcttggtc ttcatcttga ttttcaacct
    15001 ttctgcacgt tatattggta agaaacttca tgcgaaactg acttcagcag cctagaagtc
    15061 tgacttataa ggagaaaaaa tggcagatta caactggaat gaacgccata tcattacttt
    15121 ttcagaggag aatatcgccc tttcaacaaa agatgttcat gtttactatg gtaaaaatga
    15181 agcgatcaag ggcattgata tgcagttcga aaaaaataaa attacagcct tgattggacc
    15241 atcagggtgt gggaaatcaa cctatcttcg cagtcttaat cgtatgaatg atactattga
    15301 tattgctaag gtaaccggtc aaattcttta tgaaggaatc gacatcaata gaccagatat
    15361 gaacgtttac gaaattcgta agcatatcgg aatggtattc caacgtccca acccatttgc
    15421 aaaatctatt taccgtaaca tcacattcgc acatgaacgt gcaggcgtga aagataagca
    15481 ggttttggat gagattgttg aaacttctct gaaacaagca gccctttggg atcaggtaaa
    15541 agatgacttg cacaagtcag cctttacctt atcaggtgga cagcaacagc gtttgtgtat
    15601 tgcacgagct atctctgtaa aaccacaaat cttgcttatg gatgagcctg catcagcctt
    15661 ggacccgatt gcgactatgc agttagaaga aactatgttt gaattgaaga aggactacac
    15721 cattatcatt gttacgcaca atatgcaaca agcagctcgt gcaagtgatt acacagcttt
    15781 cttctatctg ggtgacttga ttgaatatga taagacagca aacgtcttcc agaatgcaaa
    15841 actcaagtca actagtgact acgtttcagg acattttgga tagaaaggga acaaatgaca
    15901 tcacctattt tacaagtaaa agatttgtct gtttactata acaagaaaaa agccttgaat
    15961 aatgtgtcaa tcgatttcta tccaaatgaa attactgctt tgattggacc atctggttct
    16021 ggtaagtcaa ccctcttgcg ttctattaac cgtatgggag atttaaaccc agaggtaaca
    16081 ttgacaggtg cgattgatta caacggaaag aacatataca gtcctcgtac ggatactgtt
    16141 gatcttcgta aggaaatcgg tatggtattc caacaaccaa acccattccc gatgtctatc
    16201 tacgagaatg ttgtttatgg tttacgtatt aacggtatca aggacaagca ggtcttggac
    16261 gaagcagttg agcgttcctt gattggtgct tctatttgga atgaagtgaa agatcgcttg
    16321 catgactcag cagttggact ttcaggtggt cagcagcaac gtgtatgtgt ggcccgtgta
    16381 ttggcaacaa gcccaaaaat tattttgttg gatgagccaa cgtcagccct tgacccaatc
    16441 tcagctggta aaattgagga cacactttat ggtctgaaag ataagtatac aatggtgcta
    16501 gtaacacgtt ctatgcaaca ggcatcacgt atttcagatc gaacaggttt cttccttgat
    16561 ggcgatttga tagaatacaa tttgacaaaa gaaatgttct tgaatcctgc caataaagaa
    16621 acagaagact acattacagg taagttcggc taaggcctat aagaaaaaga aagaggttca
    16681 catgttaaga gctcaatttg aagaggaatt aggaaaactt cataaccaat tttacgccat
    16741 gggaaatgaa gtattggctc agattaataa tgctgttcgt gcctttacaa ctcatgatcg
    16801 tgaattagca aaagaagtca ttgaagcaga taagaaaatt aatgctttcg aagtgaaatt
    16861 agagaaaaaa tcacttgaaa tcattgctct ccaacaacct gtctcaacag atcttcgtcg
    16921 cgttatcaca gtcttaaaag caactagtga tttggagcgt atgggagacc atgcagtctc
    16981 agttgctaaa gcagctatcc gtatgaaggg agaagttcgc atcccagttg ttgaagaaga
    17041 aatcaagaaa atgggacgtg aagttcgtca attagtagaa gaaactttgg aactctactt
    17101 aggtgatgct gatatggagc gcgcttatga agtagcagca cgtgatgagg ttgtcaatga
    17161 attctattca actattcaag aattgacaac ggaagaaatc aagaagaatc cagaatcctt
    17221 ggttgctggt cgcgactatt accaagtttt aacctacttg gaacgtgtcg gtgattatgc
    17281 taaaaatatt tgtgagtggg ttgtttatct acgcacaggt gatattacag agttgtaatt
    17341 cagaagtccg agagggctt
//
LOCUS       DBNFEG010000025        17289 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.025, whole genome shotgun sequence.
ACCESSION   DBNFEG010000025 DBNFEG010000000
VERSION     DBNFEG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 17289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17289
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.025"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..1441)
                     /locus_tag="AC1PNG_000591"
     CDS             complement(<1..1441)
                     /locus_tag="AC1PNG_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP6701114.1"
                     /translation="MLLATQHLTLDHQKDLRNLVHDLNFIVNPGDKIAIIGEEGNGKS
                     SLLLTLMDSTLVADYLLISGSIHRYFHSPVYIPQSLLLEMAELTLNDYFFSDLDSDID
                     YGLLYLYADQLQFDSERFSGQQLIESLSGGEKLKIQLIKSLATPSDIIFLDEPSNDLD
                     LDTLLWLENYIITSPKTILFVSHDEDFLSRTATKIIHLESVKKKTLAQTKVEELDYQD
                     YALRRQQAYQKQAQKARNDQKEFDKAMNKHLRQKSQVRNTLRNTHDATMGRLIAKKMK
                     NVLSREKRYERMKEKLTEVPFHEDAISLFFSDISPLPARKQVLHLENFQLNVEERQLV
                     SNIHFNINGQEKIGIIGQNGIGKSSFLKLIYQNLRQRADINLGYMPQRYTEVLDESKT
                     PLDFLLENGNREQERLILTHLASLQFTRQEVHHKICELSGGQKAKLLLLKMVLEQVNF
                     LLLDEPSRNFSPTSQPYIRQLFADYPGGLV"
     gene            1741..2232
                     /locus_tag="AC1PNG_000592"
     CDS             1741..2232
                     /locus_tag="AC1PNG_000592"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6701115.1"
                     /translation="MAITGIEQADILEIDKQVRLRRYDGQHDFAFEWHQDLELVWLID
                     GDQERYSLDLLNRMYDYLSKNGECYFIEIFEDNQFIPIGDVTLFADDFAIAIGDRRYW
                     KKGIGTKVLHRMIERARDVGLEEILVEEIYDWNLGSRKLFEKCGFEALEKTKKGWSYK
                     MIL"
     gene            complement(2288..3028)
                     /locus_tag="AC1PNG_000593"
     CDS             complement(2288..3028)
                     /locus_tag="AC1PNG_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIP6701116.1"
                     /translation="MAQLKINVHDLHKSYGKNEVLKGITAKFYEGDVVCIIGPSGSGK
                     STFLRTMNLLETITSGQVTVDGYDLTDPKTDVDAFRSNVGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMDKSEAEKVGMELLEKVGLADKRDAMPDSLSGGQKQRVAIARALAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARKVANRVIFTDGGQF
                     LEDGTPEQIFDNPQHPRLKDFLDKVLNV"
     gene            complement(3028..5226)
                     /locus_tag="AC1PNG_000594"
     CDS             complement(3028..5226)
                     /locus_tag="AC1PNG_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HIP6701117.1"
                     /translation="MKKKLLMLLASILPVFFIFTGVKADDTIDIVFDNAYAPFEFKDS
                     DQIYKGLDVDIINEVAKRSGWTMNQSFPGFDAAVNAVQAGSADALMAGTTITEARKKV
                     FTFSDPYFDTKIVIATTKANTISSYKDLKGKTVGVKNGTAAQNFLEENKDKYGYNVKT
                     FDTGDLMYNSLSAGAVDAVMDDEAVIQYAIQQGQDLSIDIEGEAIGSFGFSVKKGSQY
                     EYLVEDFNKALAEMKKDGTYETIMNKWLGASTTSAESTDYSSRLSLTGNASAKATPVK
                     SSYTIVADSSFAPFEYQDESGNYVGIDMELIKAIAEQQGFTITIQNPGFDAALNAVQA
                     GQADAVIAGMSITDARKEIFDFSNAYYTSNILLAVKNGSDIASYEDLKGKTVGAKNGT
                     ASYSFLEENKAKYGYTLKAFDEASSMYDSLNSGSIDALMDDEAVLLYAIQQGRNFATP
                     IPGEKSGEYGFAVKKGANPELIEMFNNGLAALVESGKYDEILNKYFNSTEEASTTTST
                     VDETTIVGLLKNNYGQLLSGLGITIGLALLSFAIAIVIGIIFGMFAVSPVKALRVTSS
                     VFVDVVRGIPLMIVAAFIFWGIPNLIESITGQQSPINDFVAGTIALSLNSGAYIAEIV
                     RGGIQAVPAGQMEASRSLGISYGTTMRKIILPQAGKIMLPNFINQFVITLKDTTIISA
                     IGLVELFQAGKIIIARNYQSFRMYAILAIIYLVVITLLTRLARKLEKGGK"
     gene            5427..6086
                     /locus_tag="AC1PNG_000595"
     CDS             5427..6086
                     /locus_tag="AC1PNG_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701118.1"
                     /translation="MIVRYSRWKSLLALGLSLFFGLPLSYVTVDLFISLWGEDVATVI
                     LGGILFLLIFGIPTLASHLVIIVSIQRLFFGLKAFELTEKHFVLYRGIFRRKAVSIPI
                     NEIEFICLEEQTLGAGLSRLFGNVSEKTVVSVGKTIAFATGAKTSARASLSDTYTHLL
                     ILFHDSERAVFYAKQYGIKIKNLLKYTAETSILDIEPGPIEHFSEEKLQDLLEERKLN
                     K"
     gene            6083..8068
                     /gene="uvrB"
                     /locus_tag="AC1PNG_000596"
     CDS             6083..8068
                     /gene="uvrB"
                     /locus_tag="AC1PNG_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIP6701119.1"
                     /translation="MINRNTENQFKLVSKYSPSGDQPQAIETLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVIARVNKPTLVIAHNKTLAGQLYSEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYS
                     DSVVSLRPGQEISRDQLLNSLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREIESLTGKVLGDVDHLAIFPATHFVTNDDHMETAIAKIQAELEEQL
                     KIFEAEGKLLEAQRLKQRTDYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PEDYLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYEMEQTDTVVEQIIRPTGLLDPEVEVRPTMGQMDDLLGEINARVEKG
                     ERTFITTLTKKMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADKVTESMR
                     KAMEETARRRQIQMAYNEEHGIIPQTIKKEIRDLISVTKAVTQDKEEVVDFNALNKDE
                     RKAMIKKLEGQMQEAAEVLDFELAAQIRDMVIELKNM"
     gene            8083..9894
                     /locus_tag="AC1PNG_000597"
     CDS             8083..9894
                     /locus_tag="AC1PNG_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HIP6701120.1"
                     /translation="MKYFDTSKTTNQVVTCIRQLENTELEKELLQFIEECEKADSLSK
                     EYIERFFKIVDAFLEVFPGSSLDSQHLKKLLTLLHEKIKTEEVWAEIFYRDTSNISAY
                     SRLFRELIVRVSTYDQIRDFVDRGSIFGTLLESRESVSHTVTRLNLLILIYFVFTFLF
                     ISDTDKEKFKLQIDSYIQELLQCDKYLDQMALFYLLSSNKHIQILKREDLRIINQYVE
                     INIQDNEKFIQQYLDYLKELGADAIAESQKVLQDLNKYTDSDIMKSQIIELLDLTILP
                     VSEPHSSEEIVEKIKGAIDEIRSALIIDAKKLKENEFYGHYTTIDTLVNFLFKPETEE
                     NKNCPTYLRLSNANQLNDPMEGKALLAILGLDAHMEQYYKPTNVFLSSLTVVKDSLPM
                     WKEYAEESRGAFLEYDQDYLETIVKHDVIEFAKVHYITTDEKQNITGDKLVDQKLIYI
                     QDTIKYLEDEGATDEIREIIDYIAKISYLFKVSDYQYESEYRILVNLDDTHFTNKFTD
                     KLNSGIKDEATRRKLDIGIGLLENKNVNYLNSRKYIHIEKTEQGKCNLYAYINVVPLK
                     YSKIMLGPKVENVDYIAPYIHYIQPDIIVETSSIPYR"
     gene            9937..10035
                     /locus_tag="AC1PNG_000598"
                     /pseudo
     CDS             9937..10035
                     /locus_tag="AC1PNG_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261961.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
     gene            10222..10959
                     /locus_tag="AC1PNG_000599"
     CDS             10222..10959
                     /locus_tag="AC1PNG_000599"
                     /inference="COORDINATES: protein motif:HMM:NF016529.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HIP6701121.1"
                     /translation="MKNIFGEKRQRFGFRKLSIGLVSAAVASLFFASSVAAAPSASAQ
                     SINYSYVTEQELTDAEKELIIRDLPGLAQETDANYYLIYRPATGTTSTPSTSTSQVLP
                     NTGSVETGLLVAGGVSLLLLAVRFGKKGKKELAGVILLTATGASFFGPTSSALTSQIL
                     ARYNHAIEISAGQALPAPAEIDGYVYVGYLKDSKAIEQTTSEGIRTETIVNKTEVIPF
                     EIQTVENPNYLRGQSEWYRKDRMGSVL"
     gene            10923..16256
                     /locus_tag="AC1PNG_000600"
     CDS             10923..16256
                     /locus_tag="AC1PNG_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HIP6701122.1"
                     /translation="MVQEGQDGERTVTIKQVHSKGQIISEEEISSTVTKTAVSKIVEV
                     DTKQATDDIVTEVPDTAPSHELPTLQITEEVSTYTESIPFETQEVADDTLLEGTRQIV
                     QEGKAGQKTIETKKTYIDGILVKTETVSETINQPAAPQIIHVGTKPVTSVPDSAPSHE
                     VPTISLTEETHTHTEAIAFDVQEIYDANLAEGSREVEQKGQAGVRTIETKNYYADGVL
                     IKSEQVSDVVTKEPVTEVVRVGTKTTDVIGSETIVTTEELPFETTVTETEELYVGEEK
                     FITEGKVGSKEVTTTYQTINGVRQPAPTVTEKVLEEPTTKEVLKGTKPIEGTETETNQ
                     VEIAFETEYVDDPTLLESKTKVVTAGVNGSKTVTTTYQTIKGVRQENSTVTEEITKQP
                     VKQVIARGTKVEKVPQVIITDLVENDDAKSATISYKLTDETANFQRAVALLYDNTGAL
                     VQEQTITDPNGQLTLENLDFYTDYTVKTKIFYTMAEQEQSSEQEAILESIRKFDLVYK
                     KIEIKDIDAVTVYRRKNGSYIGQEFLEELPTSTDELFIKVTSDRFKEVYLPVSSIEET
                     TLNGKAVFKLVSSFDELVQDKDAQYVANREFYIPKMATDANTYTSFKALLDAMKANPS
                     GTFKLGAHLDASEVPVGDVASYVTNFSGTLDGLNDGYAFSISNLKAPLFFNLGGKVQN
                     LDIKNASLNTSSKNPLATIAINANGATITNVAVEASIKGPQNVSGLVHSATNTTIKGV
                     SFKGSIEVTGTNASLTGGILGNGTMASVGNAKVDATITLPGTENQVAGGIVGRTMLVY
                     DVPGSVYNSYATGSIVTNGTEATIGGIAGSNQVTGDLAPHVGNLTNVVSTMNGPTSLV
                     GLVVNPTGKIKDGFTTTSDTLGNVKAITNEEAQAKVEAMKIQATSSDSKPLNLNPYSV
                     NYLTLDKAQADHETAYYNMEKILPFYNKELLVYYGNKIAVDDKLNKVRLLDVVPMKDT
                     TFIADVHREKANINKIMLHYADDTVDYKAVSYLEDFKNNHVVEYTISGTDLIYTPESF
                     LNDRSALVNDLVSSLSSVVLDSDAMKAVINYPTKLNADTQTGTAKDFYFGDSYNQVMA
                     NLESNVRKILVASLNGQGQASEDYIKEKITNNKAAFVLGLTYLNRWYDINFGEMNTKD
                     LTIFEPDFFGNDVASALDMILAIGNGGYDVLRAHNNVTTFASIIGKQNNQTKLFDMLE
                     DYRQLFLPEMTNNEWFKQTTKAYVVEGKSLIPEVAAKQETTDTYSKYNVGAYSKIVND
                     TVSNPTWKYNHMLLPLLTLPQENIFIITNMNTIAIGSYEHYVDDYSTVENRDKVRQMV
                     DLAAERQRDNADFWYKILDETNRDKLFRSVLNNEGYMMFGPDGTKAYRNLTADVDAIQ
                     DFYGPINKWYREHPSIKTAYADGSETYYITYDMLSDYGTALYTHEMVHNQDGDIYLKG
                     YGRRIGQGMEVYAQGLLQNVFNVTEMNLGFNAVYNSDDANRVHVGDPVARFNSEADFN
                     EYYHNQFDVLYLLDYLEGTNILAQSDAAKKAWLRKIENYYVQNNGVDTHAGNSARALT
                     DAEVASSKSFNDLIDQSIIVQRQYVNNPTNTSKKWDRNSYVSVPMFAANFSALSNSNG
                     SPGDIMFRRMAFELIAAKGYTDGFVPYASGQLSDLAMEKGSKIYDTWTKKDVGLITDD
                     HVLEYIYQGRYATWADFKKAMFAERLAKVAAGQLKPFTMQYELGVADSTKEVTITNFE
                     QLQTLMKEAMEADIQANSLNLNNSRVHALKVKVYQALMNSTNDFRTSIFNP"
     gene            16330..16833
                     /locus_tag="AC1PNG_000601"
     CDS             16330..16833
                     /locus_tag="AC1PNG_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIP6701123.1"
                     /translation="MMIKPLETKSEILGKAYVHWKSWQEAYADLLPQEFLKNTYTLER
                     CQDWAVRYPQNILIAVMDDKVVGFACYGSSSQEDLQGAGELYALYVLADYYDQKIGYQ
                     LMQAALEKLQSYETVTLWVLEGNARAIAFYEKVGFRFDGVKKTVNLGAERTEYRMIFK
                     QKERENG"
     gene            16826..>17289
                     /locus_tag="AC1PNG_000602"
     CDS             16826..>17289
                     /locus_tag="AC1PNG_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HIP6701124.1"
                     /translation="MARKEMVTLTNMCLIEDKEGKVVVQIRDPKRYRWSGVAFPGGHI
                     EEGENFHDSVVREVQEETGLTVTDARLVGLKHWPDKEGHRYIVFLYKATEFTGTIRST
                     EEGEVRWVEKSDLPQMDLAYDLLEILKVMDEPDLSEFFYTRKNDDGEWDKNF"
BASE COUNT         5340 a         3200 c         3838 g         4911 t
ORIGIN      
        1 ataccaaacc acctggataa tcggcaaata gttgacgaat atatggctga gaagtaggcg
       61 agaagtttcg actgggttca tctaataaga gaaaattgac ttgttcaaga accattttta
      121 acaagagtaa cttggctttt tgtccccccg aaagttcaca gatcttgtga tgaacttctt
      181 gtcgtgtaaa ctgcagactg gctagatgcg ttaagataag tcgttcctgc tcacgattgc
      241 cattttctaa taaaaaatca agtggagttt tagactcatc aaggacttct gtatagcgct
      301 ggggcatgta tcccaagttt atatctgctc gttgcctgag gttctgatag atgagcttta
      361 gaaaactgga tttaccaatt ccattttgtc caataatgcc aattttctct tgtccattta
      421 tgttgaaatg gatattgcta actaattgtc gttcttcaac gttcaattgg aagttttcta
      481 agtgaagtac ttgctttcta gctggtagtg gtgaaatgtc tgagaagaag agggaaatgg
      541 catcttcatg aaaaggaact tctgtgagtt tttccttcat tctttcatag cgtttttcac
      601 gtgataagac attcttcatt ttcttggcaa taaggcgtcc catagtagca tcgtgtgtat
      661 tgcgcaaggt attgcgcacc tgtgattttt gtcgtaggtg cttattcatg gctttgtcaa
      721 attctttttg gtcattccga gctttttgag cttgtttctg ataggcttgt tggcgtctca
      781 atgcgtaatc ctggtaatct agttcttcaa ccttggtctg tgcaagtgtt ttcttcttaa
      841 cagattccag gtgaatgatt tttgtagctg ttctgctaag aaagtcttca tcgtgagaaa
      901 caaatagaat tgtttttggg ctcgtgatga tatagttttc aagccaaagt aaggtatcta
      961 aatccaagtc attggacggc tcatccagaa aaataatatc agatggagtt gctagagatt
     1021 tgattaactg aatttttagt ttttcaccac ctgacaggct ttcaatcaat tgttgaccgg
     1081 aaaatcgctc actgtcaaac tggagctggt cagcatagag gtagagtagg ccgtaatcaa
     1141 tgtcgctgtc caagtcagag aagaagtagt cgttcaaggt tagctctgcc atttccagta
     1201 gaagactttg gggaatatag actggtgagt gaaaatatcg gtgaatgctg cctgatatga
     1261 gtaggtagtc ggcgacaaga gttgaatcca tcaaggttag taatagacta gatttgccat
     1321 ttccttcttc cccgatgatg gctattttat cgccagggtt gacaatgaag tttaggtcat
     1381 gaaccagatt tctcaaatct ttttgatggt cgagggttag gtgttgagtg gctagtagca
     1441 taaggcctcc tttacaagaa aaaacacact acctatttag tagtgtgtcg aaatctgctc
     1501 aaaaagaccc tattcaagga atagagggaa cagaaaaatg gacgcaagaa aactactaaa
     1561 taagtataga acttaaattg agtttacttg ttattgtcca tttctcctta cctcgcatac
     1621 tttatttatt ttaatatagc atatcgatag aatcttgtca atcagcgtgc tataatattc
     1681 atagatttcg atagagatat atgttaaaat gaacttattg taaatgagag aggatccgaa
     1741 atggcaatca ctggaattga gcaagcagat attttagaaa tagacaaaca agtccgttta
     1801 cgtcggtatg atggtcagca tgattttgct tttgaatggc accaagatct agaattggtc
     1861 tggctgattg atggtgatca agaacgttat agtcttgact tactcaatcg tatgtatgat
     1921 tatttgtcta aaaatggcga atgttacttt attgaaattt ttgaagacaa tcagtttatc
     1981 ccaattggtg atgtgacgct gtttgcagat gattttgcca ttgctattgg agataggcgg
     2041 tattggaaaa agggaatcgg gactaaggtg ttacatcgaa tgatcgagcg tgcaagggat
     2101 gtgggtcttg aggaaatact tgtcgaagaa atatacgatt ggaatcttgg ttcacgaaag
     2161 ctatttgaga aatgtggctt tgaagctctt gaaaagacca agaaaggttg gtcctataag
     2221 atgatattgt aaattaattt tgagatacca aaaaaacctt gacgcaagtc aaggcttttc
     2281 gtttagctta gacgttcaag accttatcaa ggaagtcttt gagacgtggg tgttgtgggt
     2341 tgtcaaagat ttgctcagga gtgccatctt caaggaattg accgccatca gtaaagataa
     2401 cgcggttagc aaccttgcgg gcgaagccca tttcgtgtgt gacgatcaac atggtcatac
     2461 cttgttctgc caagtccttc ataacgttga gtacatcgcc aaccatttca gggtcaaggg
     2521 cagaagttgg ttcgtcaaag agcatgatgt ctgggttcat agcgagggca cgagcgatgg
     2581 caacacgttg cttctgacca cctgaaaggc tgtctggcat agcgtcgcgt ttgtctgcca
     2641 atccaacttt ttccaataat tccatgccga ctttttcagc ttcagacttg tccatcaatt
     2701 tgtgttcgat tggtgcaaat gtgatattgt caagtacaga catatgaggg aagagattga
     2761 agtgttggaa taccattccg acatttgaac ggaaggcatc aacatctgtc ttaggatcgg
     2821 ttaagtcata tccatcgact gttacttgac cacttgtaat ggtttcaagt aagttcattg
     2881 tacgaaggaa ggtcgattta cctgaaccag aaggaccgat gatacaaaca acatcgcctt
     2941 catagaactt agcagtaata cctttgagga cctcgttttt accataagac ttgtgtaagt
     3001 catggacgtt gatttttaat tgagccatta tttgcctcct ttttcgagtt tgcgtgctaa
     3061 gcgagtcaag agtgtgataa ctaccaagta aatgatggca agaattgcat acatacggaa
     3121 ggattggtag ttacgtgcaa taatgatttt acctgcttgg aagagttcga ccaaaccaat
     3181 cgcagaaatg atagtagtat ctttcagagt aataacaaac tggttgataa agttaggcaa
     3241 catgatttta ccagcctgtg gcaagataat cttacgcatg gttgttccat aggaaatacc
     3301 caaactacga gatgcttcca tttgacccgc aggaactgcc tgaataccac cacgaacaat
     3361 ctcagcaata taggcaccag agttgaggga tagggcaatc gtaccagcga caaagtcatt
     3421 gattggagat tgttgaccag taatcgactc gattaagttt ggaatgcccc agaagatgaa
     3481 tgcagcgaca atcatgagcg gaataccacg aacaacatct acaaaaacag atgaagtaac
     3541 acggagtgct ttaactgggc tgacagcaaa cataccgaag ataataccga tgacaattgc
     3601 aatagcaaat gatagaaggg caagtccgat tgtgatacca agacctgaga gcaattgacc
     3661 gtagttgttt ttcaaaagac caacaattgt tgtctcatca acagttgaag tcgttgtgct
     3721 tgcttcttca gttgagttga aatacttatt aaggatttca tcatacttgc ctgattcaac
     3781 tagggcagct aagccattgt taaacatttc aattaattct gggttggcac cttttttaac
     3841 ggcaaatccg tactcacctg atttttcgcc aggaattggt gttgcaaagt tgcgaccttg
     3901 ttggatggca tatagaagaa ccgcttcgtc atccataaga gcatcaatag aaccagagtt
     3961 taaactgtca tacatacttg aagcttcgtc gaaggctttt agggtgtaac catattttgc
     4021 cttgttttct tctaagaaac tatatgaagc agtaccattt ttagctccaa cagttttccc
     4081 ttttaagtct tcgtaagaag caatgtcact accattttta acagctaata ggatgtttga
     4141 agtatagtag gcgtttgaga agtcaaatat ttccttacga gcatctgtga tagacatacc
     4201 ggcaataact gcatcagctt gtccagcttg aacagcattt agtgcagcgt cgaaaccagg
     4261 gttttgaatg gtaatagtaa atccttgttg ttcagcgatt gccttgatta attccatgtc
     4321 aatcccgaca tagttacctg attcatcttg gtattcaaat ggagcaaaag aagaatctgc
     4381 cacaattgtg tagcttgatt tgacaggtgt tgctttagct gatgcgtttc ctgttaagct
     4441 taaacgagat gagtaatctg tactttcggc agaagtggta gaagctccta accatttgtt
     4501 catgattgtt tcgtaggtgc catctttctt catctcagct aaagccttgt taaagtcttc
     4561 aacaagatat tcatattggc tacctttttt tacagagaaa ccaaatgaac cgattgcttc
     4621 gccttcgatg tcaatgctta ggtcttgacc ttgttggata gcgtattgaa taaccgcttc
     4681 gtcgtccatg acagcatcaa cagcaccagc agaaaggcta ttatacatca aatcgccagt
     4741 atcaaaagtt tttacgttgt atccgtactt gtctttgttt tcttctagga agttttgggc
     4801 tgcagtaccg tttttgacac cgacagtctt gcctttaagg tccttatatg aagagatggt
     4861 atttgctttt gtggtagcga taacgatttt ggtgtcgaag taagggtctg agaaagtaaa
     4921 tactttctta cgcgcttcgg tgatagttgt acctgccatt agggcgtccg cagatccagc
     4981 ctgtacagcg tttacagctg catcaaatcc tgggaagctt tgattcatgg tccaacctga
     5041 gcgcttggct acttcgttga tgatgtcaac atctaatcct ttataaattt gatctgagtc
     5101 tttaaactcg aatggggcgt aagcattgtc aaatacgata tcaatagtat cgtcagcttt
     5161 aacgccagta aaaataaaga atacaggcaa aatgcttgcc aataacatga gtaatttttt
     5221 cttcatttcc atactccttc tattataaaa taactgtcct agtatatcat attctgaaaa
     5281 ttaatgcaaa atcgaggttt ttataatgtt agaatttctg acatgcaaag tcattaaaat
     5341 gtgagaaaat ttaatgtttt agggcaattg attttgagta agttcaagcg gacttgtggt
     5401 aaaatagaat agtgaaagga atgagtatga ttgttcgtta tagcagatgg aaatctttgt
     5461 tggccttggg tctgagtttg ttttttggct tgccattgtc ttatgtaacg gttgatttat
     5521 ttatcagttt atggggagaa gatgtagcga cagtgatcct cggtgggatt ttatttttac
     5581 tgatctttgg cattccaacc ttggcttcac atctggttat tatcgtttct atacagcgcc
     5641 tcttttttgg attaaaagca tttgaactga cagagaagca ctttgttctc tatcggggaa
     5701 tatttcgaag aaaagctgtg tccattccta taaacgaaat tgagtttatc tgcctagaag
     5761 aacagacact aggagcaggc ttgagccgtc tttttggaaa tgtttctgaa aaaacagtag
     5821 tatctgtagg gaagacaatc gcatttgcga ctggggctaa aacgagtgca agggccagtt
     5881 tgtctgacac ctacacgcac ttattgatat tgtttcatga cagtgagcga gctgtctttt
     5941 atgctaaaca atatggaatt aagataaaga atttattgaa atacacggcg gaaacttcga
     6001 ttcttgatat tgaacccggc ccaattgagc atttttccga agagaagttg caagatttac
     6061 tagaagaaag aaaactgaac aaatgataaa tcgaaatact gaaaaccaat ttaaacttgt
     6121 ttctaaatac tcaccgtctg gtgaccagcc ccaagccatt gaaaccttgg ttgataacat
     6181 cgaggggggc gaaaaagctc agattctcat gggggcgact ggtacgggta agacctacac
     6241 catgagtcag gtcattgccc gtgtcaataa gcccactctt gtcatcgccc acaacaagac
     6301 tctagctggt cagctctaca gcgagttcaa ggaattcttc ccagaaaatg cggtcgaata
     6361 cttcgtatct tactatgatt actaccagcc agaagcctat gtaccgtcta gcgataccta
     6421 tatcgagaag gacagttcgg tcaatgatga gattgacaaa cttcgtcatt cagcgacttc
     6481 agcacttctc gaacgaaatg atgtcatcgt tgtggcttcg gtttcctgta tctacggttt
     6541 gggatcgccc aaggaatatt cagatagcgt ggtcagcctg cgaccaggtc aggagatttc
     6601 ccgtgatcag ttgctcaact ctctggtaga tattcagttt gagcgcaacg acatcgactt
     6661 ccaacggggg cgtttccgtg tacgtggaga tgtggtggag attttcccag cttcccgtga
     6721 tgaacacgcc tttcgtgtgg agttttttgg tgatgaaatc gaccgcattc gtgagattga
     6781 aagcctgaca gggaaggttt tgggcgatgt ggatcacttg gccattttcc ctgccaccca
     6841 tttcgtgacc aacgatgatc acatggaaac ggctattgcc aagattcagg cggaactgga
     6901 agagcagctc aagatctttg aggcagaagg aaaactctta gaagctcagc gattgaaaca
     6961 acggaccgac tacgacatcg agatgcttcg agaaatgggc tacaccaacg gagttgagaa
     7021 ctattcacga catatggacg ggcgaagcga gggcgagcct ccctacaccc tgctggactt
     7081 tttccctgaa gattacctga ttatgattga cgagagtcat atgaccatgg ggcagattaa
     7141 gggaatgtac aatggtgacc gctcacgcaa ggagatgttg gtcaactatg gtttccgcct
     7201 cccgagtgca ctggacaacc gtccgctgcg cagggaagaa tttgagagcc atgtccacca
     7261 gattgtctat gtatctgcga cgccgggtga ctatgaaatg gagcagaccg ataccgttgt
     7321 cgagcagatt attcggccga cagggctttt ggatccagag gtggaagtcc gtccaaccat
     7381 gggccaaatg gacgacctct tgggcgaaat caatgcccgt gttgagaagg gtgagcggac
     7441 ctttatcact accctgacca agaaaatggc agaggacttg accgactacc tgaaagaaat
     7501 gggcgtcaag gtcaagtata tgcactcgga catcaagacc ttggagcgta cggagattat
     7561 ccgtgatctt cgcttgggtg tctttgatgt tttgataggg attaacctgc ttcgtgaagg
     7621 gattgacgtg ccagaagtta gtctagtcgc tatcttagac gcagacaagg agggcttcct
     7681 ccgtaatgaa cgggggctca tccagacaat cggtcgagcg gctcgtaact ctgaaggtca
     7741 tgtgattatg tatgcggaca aggtcaccga gtccatgcgg aaggccatgg aagaaacagc
     7801 ccgccgccgt caaatccaga tggcttataa tgaagaacat ggcatcattc cacaaactat
     7861 taagaaagaa atccgtgacc tgataagcgt gactaaggct gtcactcagg acaaggaaga
     7921 agtagtggac ttcaatgctc ttaataaaga tgaacgcaag gctatgatca agaaactgga
     7981 aggtcagatg caggaagcag cagaagtgct tgactttgaa ctggcagctc aaattaggga
     8041 tatggtcatt gagttgaaga atatgtagat atggaggtag ttatgaaata ttttgatacg
     8101 tcgaaaacaa ctaatcaagt ggtaacctgt attagacaat tggaaaatac agagctagaa
     8161 aaagagttgt tacagtttat tgaagagtgt gagaaagcag actctctatc caaagagtat
     8221 atagaacgtt tttttaaaat tgtcgatgct tttcttgagg tgtttccagg atcatccttg
     8281 gattcgcagc acttaaagaa actcttgact ttgctccatg aaaaaataaa aacagaagag
     8341 gtgtgggcgg aaatctttta tagagatacc tctaacatta gtgcttattc tagattattt
     8401 agggaattaa ttgttcgagt tagcacttat gatcaaatta gagattttgt tgatagggga
     8461 agtatttttg ggacgctatt ggaaagtaga gaaagtgtga gtcatactgt aaccagactc
     8521 aatttactaa tactgatata tttcgtattt acctttttgt ttatttctga tacggataaa
     8581 gaaaagttta aattacagat agattcctat attcaggaat tacttcaatg tgataagtat
     8641 ttagaccaaa tggccttatt ttatttgcta tccagcaata aacatattca aatattgaag
     8701 agagaagact tacgtataat taatcaatat gtagaaataa atatacagga taatgagaag
     8761 tttattcaac agtaccttga ctatctaaag gaattggggg cagatgcaat tgcagaatct
     8821 cagaaagttc tacaagactt gaataagtat acggattctg acattatgaa aagtcagata
     8881 atagagttac tagatttaac tatcttgcct gttagcgaac ctcattcaag cgaggaaatc
     8941 gttgagaaaa taaagggagc tattgatgaa attcgttctg cattgataat tgatgcaaag
     9001 aaattaaaag agaatgaatt ttatggccac tatactacaa tagacacctt ggttaatttc
     9061 ttatttaaac cagagacgga agaaaataaa aattgtccaa cctatttacg tttgtcaaat
     9121 gctaatcaat tgaatgatcc gatggaagga aaagccttac tcgccatcct tggtttagat
     9181 gcccatatgg aacagtatta taaaccaacc aatgtgtttc tttcatcctt gacagttgtt
     9241 aaagatagtt tacctatgtg gaaagaatat gctgaggaat ctaggggagc atttctggaa
     9301 tatgatcaag attacttgga aactattgtc aagcatgatg ttattgaatt tgctaaagtt
     9361 cattatatta ctacagatga aaagcaaaat attactggtg ataaactagt tgatcaaaaa
     9421 ctaatatata ttcaagatac aattaagtat ttagaagatg agggagcaac cgatgaaatt
     9481 cgagaaatca ttgattatat tgctaagatt tcctatcttt ttaaggtttc tgattatcaa
     9541 tatgaatctg aatatcgtat attggtaaac ttagatgata cgcatttcac taacaaattc
     9601 actgataaat tgaactctgg aataaaagat gaagctacac gtaggaaact agatattggc
     9661 ataggattgt tagaaaataa gaatgtgaat tatctgaaca gcagaaaata tattcacata
     9721 gaaaaaacag aacaagggaa atgtaattta tatgcgtaca tcaatgttgt cccattaaaa
     9781 tattcaaaga ttatgttggg gccaaaagtt gaaaatgtag actatatagc tccttatatc
     9841 cattacattc agcctgacat tatcgttgaa acttcaagta ttccgtatcg ataacctctt
     9901 ttgaaaagct acgaagaatt gagttagacc ataagtaaga aactggaagg tcagatgcag
     9961 gaagcggcag aagtgcttga ctttgaactg gcagcccaga ttcgtgatat ggtcatcgag
    10021 ttgaagaata tgtaataaga gttgctaagt tcagtgattt aagtagaatg aaattacaag
    10081 aggcggcagg tagaaattga cctgccacct tttataatac ttaaaatttt tacttggaga
    10141 cttagggaca aaaaatttgt ccctgtccct ataaaaggtt ataatggttt ctatactatt
    10201 tagggaatag ggggagtttt tatgaaaaat atttttggtg aaaaacgaca gcggttcggt
    10261 tttcggaaac tgtcaattgg acttgtctcg gcagctgtag caagtttgtt ttttgcgtca
    10321 tcggtcgcag cagcaccgtc agctagtgct cagtctatca attattccta tgtgacagag
    10381 caagaattga cagatgcaga aaaggagttg attattcgtg acttgcctgg cttagctcag
    10441 gaaacggatg caaattatta cctgatctat cgtccagcta ctggcacaac atctactcca
    10501 tcgacaagca cgtctcaagt tttgccaaat actggctctg tagaaaccgg actattggtg
    10561 gcgggtggag tgtctctcct gttattggct gttagatttg gtaaaaaagg taagaaggag
    10621 ttggcgggtg tcatcctttt gacagctacg ggagcaagct tcttcggtcc aactagctcc
    10681 gcattgacga gtcaaatttt ggctcgatat aaccatgcaa tcgaaatatc tgccggacaa
    10741 gcgttaccgg ccccagctga gattgatggc tacgtttatg taggatattt gaaggatagc
    10801 aaagctattg aacaaactac ttcagaagga atccgtacag aaaccattgt caacaagaca
    10861 gaggtcattc catttgaaat tcaaaccgta gaaaatccca attatctgcg gggacagagc
    10921 gagtggtaca ggaaggacag gatggggagc gtactgtaac gattaaacaa gtccattcaa
    10981 aaggtcaaat tatttcggaa gaagagattt cctcgactgt tacaaaaact gctgtttcaa
    11041 agattgttga agttgataca aaacaagcaa ccgatgatat tgttacagaa gtaccagata
    11101 cagcgcctag tcacgagtta cctaccttac agataacgga agaagtatct acctacacgg
    11161 agtctattcc ttttgaaaca caagaagttg ctgatgatac cttgctagaa ggtacaagac
    11221 agattgtgca agaaggtaaa gctggacaaa agacaattga gacaaagaaa acctacatcg
    11281 acggtatttt agtaaaaaca gaaactgtct ctgaaacgat taaccaacct gctgctccac
    11341 agattatcca tgttggaaca aagccagtca caagtgtacc agattcggca cctagccatg
    11401 aagttccaac gatttcttta acggaagaaa cacatactca tacagaagct attgcctttg
    11461 atgtgcaaga aatctatgat gcaaacttgg cagaaggcag ccgtgaagtt gaacagaagg
    11521 gacaagctgg tgttcggaca atcgagacta agaattatta tgccgatggt gtactaatta
    11581 aatccgaaca agtttcagat gtagtgacaa aagaaccagt aacagaagtt gtaagagtcg
    11641 gtacgaagac aacagatgtt attggatctg aaaccattgt aacaacagaa gagcttccgt
    11701 ttgaaacgac agtcacagaa acagaagaac tttatgttgg ggaagaaaaa ttcattactg
    11761 aaggtaaggt tggttctaag gaagttacca ctacttatca gacaatcaac ggagtccgtc
    11821 aaccagcccc aacagtgact gagaaagtcc ttgaagagcc tacaacgaaa gaggttctca
    11881 aaggaacaaa accgatagag ggtacggaaa ccgagactaa tcaagttgaa attgcctttg
    11941 aaacagagta tgttgatgac ccgacattgt tagaaagtaa gaccaaagtt gtaacggcgg
    12001 gtgtaaatgg ctctaagaca gttacaacaa cttaccagac tatcaagggt gttcgtcagg
    12061 aaaattctac tgtaacagaa gaaattacca aacaacctgt taagcaagtc attgcgcgtg
    12121 gtacaaaggt tgaaaaagta ccacaagtga ttatcacaga tttggtggaa aatgacgatg
    12181 ccaagtcagc gacaatcagc tacaagttga cagacgaaac cgctaatttc caacgagctg
    12241 ttgctctcct ctatgataat acaggtgcct tggttcaaga acagaccatt acagatccaa
    12301 atggccagtt gacacttgaa aatcttgatt tctacacaga ttacactgtt aagactaaga
    12361 ttttctacac aatggcagag caggaacaaa gttcggaaca agaagctatt cttgaaagca
    12421 tacgcaaatt tgatttggtc tacaagaaga ttgaaatcaa ggatattgat gcggtgactg
    12481 tttaccgtcg taaaaatggt agctacatcg gtcaagaatt cttggaagaa ctgccaacat
    12541 ccaccgatga attgtttatc aaggtgactt cagaccgctt caaggaagta tacctacctg
    12601 tgtcaagcat tgaagaaaca accttgaatg gcaaggcagt cttcaaacta gtttcatcct
    12661 ttgatgaatt ggtacaggac aaggatgccc aatacgttgc caaccgtgaa ttctacattc
    12721 ctaagatggc gactgatgca aatacctaca catccttcaa agctttgctc gacgctatga
    12781 aggcaaatcc aagtggtacc tttaaactag gagcgcattt ggacgcaagt gaagttcctg
    12841 ttggagatgt tgcatcgtat gtgaccaact ttagcggtac cttggatggc ttaaatgacg
    12901 gctacgcctt ttctatcagc aacttgaaag caccattgtt ctttaacctt ggtggtaaag
    12961 ttcaaaactt ggacatcaaa aatgcaagcc tgaatacaag ctctaagaac ccacttgcga
    13021 caattgcgat taatgccaat ggagcaacta ttacaaacgt agcagtagaa gcatctatca
    13081 aaggaccgca aaacgtatct ggtttagttc attctgcaac aaatactact attaaaggtg
    13141 tatccttcaa gggtagtatc gaagtaaccg gaaccaatgc aagtctaact ggtggtatct
    13201 tgggtaacgg tacgatggca agtgtcggaa atgccaaggt tgatgcgacc ataaccctcc
    13261 caggtacaga gaatcaagta gctggtggta tcgtcggtag aactatgctc gtttatgacg
    13321 taccaggaag tgtctataat tcgtatgcaa caggttcaat cgtgacaaat ggtacagagg
    13381 caaccattgg tggaattgct ggttccaacc aagtgactgg cgatcttgct ccacacgtag
    13441 gtaatttgac aaatgttgtt agcaccatga acggtcctac aagtctagtt ggtttagttg
    13501 tcaatccaac tggtaagatt aaagacggct tcacaacaac ttcagataca ttgggcaatg
    13561 tcaaggctat cacgaatgaa gaagcacaag caaaagttga agccatgaag attcaagcga
    13621 cctcgtcaga ttccaaacct ctcaatctta acccttattc tgtcaattat ttaacactag
    13681 ataaggctca ggcagaccat gaaacagcct actacaacat ggagaaaatc ttaccattct
    13741 acaacaagga actccttgtt tattatggaa ataagattgc ggttgatgat aagctaaaca
    13801 aggttcgttt actggatgtt gttccaatga aagatactac ctttattgca gatgtccata
    13861 gggaaaaagc aaacatcaat aagattatgc ttcactatgc tgatgacacg gtagattata
    13921 aggcagtttc ataccttgaa gacttcaaga ataatcatgt ggtcgagtac accatttcag
    13981 gtacggactt gatttacacg ccagaatcat tcttgaatga cagaagtgcc cttgtcaatg
    14041 acttggtaag cagcctttct tctgttgtcc tcgactcgga tgctatgaag gcggttatca
    14101 attatccaac aaaattaaat gcggatactc agactggcac agctaaagac ttctactttg
    14161 gtgacagcta taaccaagta atggctaacc tagaaagcaa cgttcgtaag attttggttg
    14221 ctagtctaaa tggtcaaggt caggcttctg aagactacat caaagagaaa atcaccaata
    14281 acaaagcagc ctttgtgctc ggtttgactt acttgaaccg ttggtatgac atcaacttcg
    14341 gagagatgaa taccaaagac ttgaccatct ttgaacctga cttcttcgga aatgatgtag
    14401 catctgccct tgatatgatt cttgcaattg gtaatggtgg ctatgatgtt cttcgtgccc
    14461 acaataacgt gaccactttt gcatccatca ttggtaaaca aaataatcag accaagcttt
    14521 tcgatatgct ggaagattat cgtcagctct tcttgccaga aatgaccaac aatgaatggt
    14581 tcaagcaaac aaccaaggct tatgttgttg aaggtaaatc cttgattcca gaagtagctg
    14641 ctaagcagga aacgacagat acatatagca agtacaatgt tggagcttat agcaagattg
    14701 tgaatgatac tgtatccaat ccaacttgga agtacaacca catgctcttg ccactcttga
    14761 ctttgccaca agaaaatatc tttatcatca ctaacatgaa taccatcgcg attggttcat
    14821 acgaacacta cgtagatgat tattcgacag tggaaaatcg tgataaggtt cgtcagatgg
    14881 ttgatttggc ggcagaacgt cagcgtgata atgcggattt ctggtacaag attttggatg
    14941 aaaccaaccg tgacaagcta ttcagatctg ttctcaataa tgaaggctac atgatgtttg
    15001 gtccagatgg taccaaggct tatcgcaatt taactgctga tgttgatgct attcaagact
    15061 tctacggtcc gatcaacaag tggtatcgtg agcatccatc tattaagact gcttatgcgg
    15121 acggaagtga aacctactat atcacttacg atatgctgtc agattatggt acagcccttt
    15181 atacccacga aatggttcac aaccaagatg gtgatattta tctgaaaggc tatggtcgcc
    15241 gtattggtca aggtatggaa gtttacgccc aaggtcttct acaaaatgtc ttcaatgtta
    15301 ctgagatgaa ccttggattc aacgctgtct ataatagcga tgatgccaac cgtgtccatg
    15361 tcggagaccc agttgcacgt ttcaatagcg aagctgattt caatgagtat taccataacc
    15421 agttcgatgt gctgtacttg ctggattacc tagaaggtac aaacatcttg gctcaatcgg
    15481 atgcagcgaa gaaagcatgg ttgcgtaaga ttgaaaacta ctatgtccaa aataatggtg
    15541 ttgataccca tgctggaaac tctgcgcgtg cattgacaga tgccgaagtg gcaagctcga
    15601 agtccttcaa tgatttgatt gatcagagta ttatcgttca acgccaatat gtcaacaatc
    15661 caactaacac aagcaagaaa tgggatagaa atagctatgt cagcgttccg atgtttgcgg
    15721 ctaacttctc agccctttca aactcaaacg gttcaccagg cgacatcatg ttccgtcgta
    15781 tggcctttga attgattgct gccaaaggct acacagatgg ttttgtgccg tatgcttccg
    15841 gacaattatc cgacctagcc atggaaaaag gttctaagat ttacgataca tggactaaaa
    15901 aagatgttgg tttgattaca gatgaccatg ttctggaata tatctaccaa ggtcgatatg
    15961 ctacatgggc tgacttcaag aaagccatgt ttgctgaacg cttggcaaaa gtagcagctg
    16021 gccagctcaa accattcacc atgcaatacg aattaggtgt tgctgattca accaaggaag
    16081 taaccatcac aaactttgaa cagttgcaaa ccttgatgaa agaagctatg gaggcagaca
    16141 tccaagcaaa tagtttaaac ttgaacaaca gtcgtgttca tgcactcaaa gtgaaagttt
    16201 atcaagcgtt gatgaactct acaaatgact tcagaacatc gatttttaac ccataatcat
    16261 aaacaagttg gtcttgtgcc aacttgtttt ttaatgtata ctagaggaaa aaattcggag
    16321 gtacattaga tgatgattaa accactggaa acgaaaagtg aaatactagg caaggcctat
    16381 gttcattgga aatcgtggca ggaggcctat gctgacttgt tacctcagga atttttaaag
    16441 aatacttaca ccttagaacg ctgtcaagac tgggcagttc ggtatcctca gaatatcttg
    16501 atagctgtga tggatgataa ggtggttgga tttgcctgtt acggctcgtc tagtcaggag
    16561 gatttgcaag gggcagggga gctctacgcc ctctatgtct tggcggacta ttatgatcag
    16621 aagataggct atcagctcat gcaggcagct ttggaaaaat tgcagagcta cgaaaccgtt
    16681 actctctggg tattggaggg gaatgcgcgt gccattgcct tctatgaaaa agtgggcttc
    16741 cgatttgatg gtgtcaaaaa aacggtcaat ttaggtgcag aacgtacaga atatagaatg
    16801 atttttaaac agaaagagag agaaaatggc tagaaaagaa atggtcactt tgaccaacat
    16861 gtgtctaatc gaagataagg aagggaaggt cgttgtacag attcgagatc ccaagcgtta
    16921 tcgttggtct ggtgttgcct ttccaggcgg acatattgaa gaaggcgaga atttccatga
    16981 ttcagttgtt agagaagtgc aagaagaaac aggtttaaca gtcactgatg ctcgcttggt
    17041 tggtcttaaa cattggccgg ataaggaagg gcatcgttac attgtgtttc tctataaggc
    17101 aacagagttt acaggaacta tccgatcaac tgaagaaggt gaggtacgtt gggtcgagaa
    17161 gtcggaccta cctcagatgg atttggccta tgatttgcta gagatactga aagtcatgga
    17221 tgagccagat ttgtccgagt tcttttatac tagaaaaaat gacgatggag agtgggacaa
    17281 gaattttag
//
LOCUS       DBNFEG010000026        17203 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.026, whole genome shotgun sequence.
ACCESSION   DBNFEG010000026 DBNFEG010000000
VERSION     DBNFEG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 17203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17203
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.026"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..845
                     /locus_tag="AC1PNG_000603"
     CDS             <1..845
                     /locus_tag="AC1PNG_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIP6701125.1"
                     /translation="KKLFSLATAALSVGVLVACGNSSSSSDSSATTVRVGVMSLSDSE
                     QARWDKVQEILGDEVKLEFTQFTDYSQPNKAVAENEVDINAFQHYNFLNNWNQENGED
                     LVAIADTYIAPIRLYSGTADGKNKYTKVEEIPDGAEIAVPNDPTNESRALYLLQAAGL
                     IKVGVSGTELATIADITENKKNLKITELDASQTASSLSSVDAAVVNNTFVLEAGLDYK
                     NALYKEQKDENSKQWYNLIAARSDWEKSEQAAAIKKIIEAYHTDEVKKVIEETSDGMD
                     EPVW"
     gene            1002..2369
                     /locus_tag="AC1PNG_000604"
     CDS             1002..2369
                     /locus_tag="AC1PNG_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIP6701126.1"
                     /translation="MADSKVQAFENDAIIQNYFEKLKVLISKKSIFAQQIGLLDVATY
                     LKDMFEEAGADVVLDDSYAAPFVMATFKASVPDAKTLIFYNHYDTVPADADQVWEKGN
                     PFELTISDGYIYGRGVDDDKGHITARLSALKKYQARQNGDLPVNVIFIMEGAEESASV
                     DLDKYLSKYKEYLIGADLLVWEQGHRNSLHQLEIAGGNKGIVTFDLQVKSADLDIHSS
                     YGGVIDSASWYLLSALQSMRAADGEILVDGIYEQVQEPNERELALVEEFALATSQSMT
                     DIYGLTLPTLVEDRREFLKRLYFEPSITIEGLSTGYLGQGVKTIIPAQASAKMEVRLV
                     PGLEPHDVLDKIRQHLDKHGFDKVELVFTLGEMSYRSDMSHPAIVNVIELAKKLTPEG
                     VAVLPTSPGTGPMHTVFHALGVPIAGFGLGNANSRDHAGDENVSIADYYSHVELVEEL
                     IASYE"
     gene            2362..3426
                     /locus_tag="AC1PNG_000605"
     CDS             2362..3426
                     /locus_tag="AC1PNG_000605"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HIP6701127.1"
                     /translation="MSKEMIKLDNIDVTFQQKKRTIEAVKDVTIHINQGDIYGIVGYS
                     GAGKSTLVRVINLLQVPTAGKITIDEDVIYEGEKVTLTPTQLRSKRREIGMIFQHFNL
                     MAQMTAEENVAFALKHSGLSKEEKKEKVAKLLDLVGLSDRAENYPAQLSGGQKQRVAI
                     ARALANDPKILISDESTSALDPKTTKQILALLQELNEKLGLTIVMITHEMQIVKDICN
                     RVAVMQDGRLIEEGSVLEIFSHPKEELTQDFIKTATGIDEALVKIYQQDIVKNLPENS
                     ILVQLKYAGSNTDTAIVNDLYKFYQVSANILYGNIEILDHTPVGEMVVILSGEPGQLH
                     RAIEAVTEARVEVTILKGAN"
     gene            3427..4119
                     /locus_tag="AC1PNG_000606"
     CDS             3427..4119
                     /locus_tag="AC1PNG_000606"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HIP6701128.1"
                     /translation="MLEWIQTNFPDIYKLGWDGQTGWLTHFNLTLYMTFVSFAFGGFM
                     GLVSGLFLVLTGPRGVIANKTAYWILDKVASIFRAIPFIILLAAIAPFTKIIVGKTIG
                     TEAALVPLALSVFPFFARQVEVVLSELDRGVIEAAQASGATFWDIVLVYLREGLPDLI
                     RVTTFALVSLVGYTAMAGAIGAGGLGQVALSYGYLRYNNDVTFLATLLILVIIFVIQF
                     IGDFLTRKISHR"
     gene            4301..5005
                     /locus_tag="AC1PNG_000607"
     CDS             4301..5005
                     /locus_tag="AC1PNG_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B3/B4 domain-containing protein"
                     /protein_id="HIP6701129.1"
                     /translation="MSQFVVDSSFWSLFPDAKIGVLLLKDYKTPAQSPDELVKLLEES
                     NGIAQKFLTEDTFSENEVIRTYRQAYQKFKTKKGARSSIEALLKRSTSDRPVTTISPL
                     VDIYNAASLRFGLPCGAEDLDTFVGNLQLTVTEGGDEFYLIGDETNNPTLPGEVCYKD
                     DKGAVCRCFNWRDGERTMITDETKNAFLVMELVNSDRVEDLENALDFISQQAETFLGV
                     VPEKYLLDCDRPGIAL"
     gene            5356..5751
                     /locus_tag="AC1PNG_000608"
     CDS             5356..5751
                     /locus_tag="AC1PNG_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HIP6701130.1"
                     /translation="MFTLEAINHGHEQFTGPDFPKLIAYFKDLGMVENIVDIQSGQVT
                     YRSQTGQTLEKTGYQVTIPVSDQANLDQFVTILRNHQAGQTDFPTFCQETAEAGVYKW
                     VIDLEAMTCSYLDQAEQAVFVEAVPSVEN"
     gene            5957..6874
                     /gene="glyQ"
                     /locus_tag="AC1PNG_000609"
     CDS             5957..6874
                     /gene="glyQ"
                     /locus_tag="AC1PNG_000609"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HIP6701131.1"
                     /translation="MSKKLTFQEIILTLQQFWNEQGCLLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLER
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEVSDQDMLLENFT
                     KFEKEAERALEEGLVHPAFDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEATRAELLKEDAE"
     gene            6874..8910
                     /gene="glyS"
                     /locus_tag="AC1PNG_000610"
     CDS             6874..8910
                     /gene="glyS"
                     /locus_tag="AC1PNG_000610"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIP6701132.1"
                     /translation="MTKNLLVELGLEEIPAYIVTPSMHQLRDRMATFLTDNRLAFDSI
                     DVFSTPRRLAVRVRGLADQQTDLTEDFKGPAKKIALDADGNFTKAAEGFVRGKGLTTA
                     DIEFREIKGEEYVYVTKHEAGQPAKAVLAGIPEVLKAMTFPVSMNWASNKFAYIRPVH
                     TLTVLLDDEALDMDFLDITSGRISRGHRFLGNETEIASADSYEADLRAQFVITDPAER
                     QNMIVEQIKAIEDKHNVTVEIDEDLLNEVLNLVEYPTAFMGSFDTKYLEVPEEVLVTS
                     MKNHQRYFVVRDKAGKLLPNFISVRNGNDQYLDNVIKGNEKVLVARLEDGEFFWREDQ
                     KLKIADLVERLKVVTFHEKIGSLYEHMERTKVIAEKLADLAGLSAGEKADVARAADIY
                     KFDLLTGMVGEFDELQGIMGEKYALLAGEKPAVAAAIREHYLPNSAEGELPESKVGAV
                     LALADKFDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILEAFGWEIPLDQLIAELYS
                     LNFASLTYDNQPAVMDFIRARVEKMMDKTIPKDIREAVLASSTFVVRLQLAASSAIFQ
                     KAKEADYKEAVENLSRVFNLAEKAEVTAIDEALFENDQEKALAAAVAGLELTEDMAGN
                     LDKLFALSPVIAAFFDNTMVMVDDAAVKANRLTLLKALADKASAVAVFNLLNSK"
     gene            8920..9177
                     /locus_tag="AC1PNG_000611"
     CDS             8920..9177
                     /locus_tag="AC1PNG_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HIP6701133.1"
                     /translation="MEQAKIDRINELARKKKAEGLTEEELQEQAALREEYIEGYRRSV
                     RAHIEGIKVVDEDGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            9316..9687
                     /locus_tag="AC1PNG_000612"
     CDS             9316..9687
                     /locus_tag="AC1PNG_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701134.1"
                     /translation="MKKLLYFLLFLTYIACAYSTIFFLPFMYFSGIVGATGVGLGLSY
                     SLHQEVKKHPEKQLSEYLPWGSKRDKIMVVTILLFSILVSFIPDVNWHRGLAILLDIS
                     YVLLWQAMLIQFLRKYYFKNA"
     gene            complement(9709..11046)
                     /locus_tag="AC1PNG_000613"
     CDS             complement(9709..11046)
                     /locus_tag="AC1PNG_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIP6701135.1"
                     /translation="MNDLTKGKPIAVILQFAIPLLIGSFFQLAYNFADSMIVGHTLGK
                     DAFASVGSTASLIFLIIGFAQGVTNGLTIISAQRFGAGDLEGLKKSFVHGLFYASLIS
                     LLLTVSALAFLKPILVLMQTPVSIIDHSHAFLTAMFGGLTFTIFYNFLSSALRSLGNS
                     KTPLLALVIACFINIGLDFFFILVMNWGVFGAGFATILAQACSVLFLIFYIIHKVPHY
                     HIGLADLKLDRGNLKKHAQLAFPMGFQASIIAIGAMTLQFMVNQLGTDAIAAQAIALR
                     TDQLAMLPMVNLGLAIATFTAQNYGAKLYDRIREGVRHSLLLSIAWAIVFAVILILGN
                     RFFSGLFLPNASQTVLDLALVYYIINGSCYWIVASLFILRSFIQGLGKGFIPTLAGFG
                     ELIFRAAVAIIGMQYFGFYGTAAANPAAWIGSIIVLIPSSIIFMKKLKAGQSI"
     gene            complement(11366..12031)
                     /locus_tag="AC1PNG_000614"
     CDS             complement(11366..12031)
                     /locus_tag="AC1PNG_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIP6701136.1"
                     /translation="MPNYTIGILGLGVFGTTIAKTLHNYDCNIIAIDNHEQRINHLEP
                     ILTRGIVGDITDRSLLRAAGIGNCDAVVVATGENLESSVLAVMHSKVLGVPMVIVKVK
                     GTTAKEVLLRVGADKVISPERETGISLAKQLLHRDTTSLFELEGNVSIVEFHPPVKWI
                     GKTLGELKLRQHYKLNIIGYRSGENQELNIQLTPDYVFKSDELILAVTDHNTVDHFEE
                     LTN"
     gene            complement(12043..13392)
                     /locus_tag="AC1PNG_000615"
     CDS             complement(12043..13392)
                     /locus_tag="AC1PNG_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIP6701137.1"
                     /translation="MSPFRIKLSPSQRIIISFLFVILTGSILLSLPISQISTSTASYW
                     DHLFTSVSMVCVTGLFTKAVAETYSTFGQIICMMLIQIGGLSLIGFIGLFALRGGRKM
                     NFINMATLQEALNRSDTKYFRNFLKSAFGFTLAVEAFGALLLSFHFVPEFGWARGLFT
                     SIFVAISAFCNAGFDNFGATSIMRYVDNPLINITLASLIIMGGLGFSVWFDLQTQIGQ
                     KRSLRKLGFHTKIVLGLTAIILTLGTLTTLLTEWNNPSTIGQLSFGHKLLASFFQTVT
                     MRTAGFASIDYTKAEPITLLLYIFQMMLGGAPGGTAGGIKITAFLTLVLYARSEILGL
                     PHTNFMGRTIDNLSIRKAFATFSVFLLMFIIGLFALALTDDRQPLLFLMFEVMSALAT
                     VGVTANLTPSLTMAGQAVIMALMFFGRIGPLSILASLSVRKLSKTESLQYAKSSMLV"
     gene            complement(13656..14195)
                     /locus_tag="AC1PNG_000616"
     CDS             complement(13656..14195)
                     /locus_tag="AC1PNG_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HIP6701138.1"
                     /translation="MNSKRQTLNFITMLAAQAVVISLIERLITPPFAFAPGAKLGLGN
                     LISLIAIFTLPTKDSLKVVALRLLISTFLGGTFSTFLYGFAGTTLSYVGMLSAKQLGP
                     NRVSPIGISILGGMLHNLGQLLVFATIARSFYVLNYLPILAFTGILSGLLVGLAATYL
                     LQKVGPLRHYHQLVLAEWK"
     gene            14378..16270
                     /locus_tag="AC1PNG_000617"
     CDS             14378..16270
                     /locus_tag="AC1PNG_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIP6701139.1"
                     /translation="MTKNIVIVGAGYAGIAAARLLGKTFKKDQDVTVTLIDKNSFHTY
                     MTELHEVAAGRVEANAIKYDLQRIFKKYPKVQLVTDKVVEIDYDKKQVVAEHQTLDFD
                     YLLLAMGGEANDFGVKGVKEHGFTLWSIEAAERLHDHMIDACYRAMREHDEAKRRALL
                     TFTVIGAGFTGIEMIGELIDWVPILAREFKLDPKEFSLKVVEATPNILAMVTEKEQVK
                     ARKYLEKKGVELVLGDGVASVQEDSLTLSSGRQIPTYTSIWTAGVQANTDASEFGIEK
                     ARAGRLVANEFMEAKGKENVYVAGDLVYFEESEGKPTPQIVQAAEQTGHTAARNIISA
                     IKGGEKHSYKGKYDGFMVSIGARYGVAFLMDKYHMSGFMAMAVKHMVNLLYFFTIRSF
                     FYMGSYVRHEFFDIQNKRNIFGGHTSGKGNLLWSVPMRVLYGSVWLYEGIKKAFGLFG
                     TTSWFGDQVVFPFPWLADPVSGASAAEAVSSASQAATAAAEAAEPIFGLSYAYGEAPM
                     AVLDKMPDWFATIMEFMMPNQEVALFMQKFMTVAEIGIGLALIAGAFVWIVSAATVAL
                     VVMFSLSGMFYWVNIWFIPAAISLMNGAGRAFGLDYWIMPWLGRFLDKKIYGKPKHIY
                     RIKYKK"
     gene            16289..>17203
                     /locus_tag="AC1PNG_000618"
     CDS             16289..>17203
                     /locus_tag="AC1PNG_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIP6701140.1"
                     /translation="MVHQIWNTYPEIERGLEEVKSIILSEMMVLHPAVTSKIVEYVEA
                     PGKYLRAGLCLLFAQMTEGKISRSKLYFAAHLEVLHLATLIHDDVIDGADKRRGVTAA
                     HQQFSNRIAIYTGDYLLAYSSRLFGKGLRLLEVSRDDLDLGNDKLMETILRGELSQLL
                     NQFDANMTMKAYLKQIQGKTAFLFGLACQLGSFVPGQSRKDSSMAFRSGQALGMAFQL
                     RDDLIDYQLDVAESGKPRLQDIQNGIYTAPLLFAMREDKSIQQQLRAYIQSPTAEGLE
                     ILTDRIFATHAIAKTEKLIVSYLEKMMNF"
BASE COUNT         4987 a         3278 c         4145 g         4793 t
ORIGIN      
        1 taaaaaaatt gtttagtttg gcaacagctg ctttatcagt aggtgtactt gttgcatgtg
       61 gtaactcttc ttcaagttca gattcatctg caacaactgt tcgagtaggt gtgatgagct
      121 tgagcgattc tgagcaagcc cgttgggata aagttcaaga aattcttggt gatgaagtga
      181 aattggagtt cactcaattt acagattact cacaaccaaa caaggcagtt gctgaaaatg
      241 aagtagacat caacgcattc cagcactaca acttcttgaa caactggaat caagaaaatg
      301 gtgaagactt ggttgctatt gcggatactt atatcgcacc aatccgtctt tactcaggta
      361 cagctgatgg aaaaaacaag tacactaaag tggaagaaat tccagatggt gcagaaattg
      421 cggttccaaa cgacccgaca aacgaaagtc gtgctcttta ccttcttcaa gcagctggtt
      481 tgatcaaggt tggtgtatct ggtacagaat tggcaactat tgctgatatt actgaaaaca
      541 agaaaaactt gaaaattact gagttggatg caagccaaac agcaagttca cttagctcag
      601 ttgatgcagc agttgtaaac aatacatttg tgttggaagc tggtttggat tacaaaaatg
      661 ccctttacaa agagcaaaaa gatgaaaatt caaaacagtg gtacaacttg attgcagctc
      721 gtagcgattg ggaaaaatca gagcaagcag cagcgattaa gaaaattatc gaagcctacc
      781 acacggatga agtgaaaaaa gttattgaag agacttcaga cggtatggat gagccagttt
      841 ggtaaaatgt aaataggttg aggactgggc agaattgttc agccctctat ctgtttttat
      901 agagataaga aaaaatctct taaccaaagt cagaaatgtg ggaggatatt tgattttggt
      961 tagtagattt aattgatttt cattgagtat gaggagaaag tatggcagat agtaaagttc
     1021 aagcatttga aaatgatgcg attattcaaa actattttga aaagttgaag gtcttgattt
     1081 caaagaaatc tatttttgct cagcagattg gtttgttgga tgtggcgact tatttgaaag
     1141 atatgtttga agaggctggg gcagatgttg ttttagacga cagctatgcc gcaccatttg
     1201 tcatggcaac gtttaaggct tcagtgccag atgcgaaaac attgattttc tacaatcatt
     1261 atgatacagt acctgcagat gccgatcaag tctgggagaa gggcaacccc ttcgaattga
     1321 ccatttccga tggctatatc tatggtcgcg gggtagatga tgataagggc catatcacag
     1381 ctcgtctgtc ggccttgaaa aaatatcaag ctagacagaa tggcgattta ccagtcaatg
     1441 tgatttttat tatggagggg gcagaggagt cagcttctgt cgacttggac aaataccttt
     1501 caaaatataa agaatatttg attggtgctg atttgcttgt ctgggagcag gggcatcgca
     1561 atagcctaca tcagctagaa attgctgggg gaaataaggg aattgtgacc ttcgatcttc
     1621 aggtcaaatc agctgacttg gatattcatt cttcctatgg aggtgtcatt gattcggcaa
     1681 gctggtattt attatcagca cttcaatcca tgcgggctgc agatggggaa attctggtag
     1741 atggcattta cgagcaagtg caagagccaa atgagcgtga attggccttg gtggaagaat
     1801 ttgcactggc taccagtcag tctatgactg atatttatgg actaaccctt ccaaccttgg
     1861 tagaagatcg tcgtgagttt ctgaagcgtt tgtattttga accatcgatt accattgaag
     1921 gcttgtcaac tggctatctt ggtcaaggtg tcaagaccat cataccagct caggcttctg
     1981 ctaagatgga agtccgtctg gtacctgggt tggagccgca tgatgtatta gataagattc
     2041 gacagcattt ggacaaacat ggttttgaca aagttgagct tgtcttcact ctaggagaga
     2101 tgagctaccg aagtgatatg tcgcaccctg ccattgtcaa tgtgattgaa ttggccaaaa
     2161 aattgacacc agaaggagtt gctgtcttgc cgacgtcacc ggggacagga cccatgcaca
     2221 cagtctttca tgctctaggt gtgccgattg caggatttgg tctaggaaat gccaacagtc
     2281 gcgaccatgc aggtgatgaa aatgtcagca tcgctgacta ctatagccat gttgaattag
     2341 tagaggagtt aattgcaagt tatgagtaag gaaatgatta agctagataa tattgatgta
     2401 actttccagc agaaaaaacg tacaatcgaa gcggtaaagg atgtgaccat ccatatcaat
     2461 caaggggata tttacggtat tgtagggtat tctggagcag ggaaatctac ccttgttcga
     2521 gtgattaatt tattgcaggt gccgacagcg ggtaaaatta ctatcgatga ggatgtaatt
     2581 tacgaaggag aaaaggtcac acttacccca actcaattgc ggagcaaacg ccgtgaaata
     2641 ggcatgattt tccagcattt caatctgatg gcgcaaatga cggcagaaga aaacgtagcc
     2701 tttgccctta agcattcggg tttgagcaag gaagagaaga aagaaaaagt agccaaactc
     2761 ttggacttgg ttggactttc tgaccgtgct gaaaactatc cagctcagct gtctggtggt
     2821 caaaagcaac gtgttgccat tgcgcgtgcc ttggccaatg acccaaaaat cttgatttca
     2881 gatgagtcaa cttctgcctt ggatccgaaa acaactaagc agattttggc actcttgcag
     2941 gaattgaatg aaaaattggg cctgaccatt gttatgatta cccatgagat gcagattgta
     3001 aaagatattt gtaaccgtgt ggcggtcatg caggatggtc gcttgattga agaaggttca
     3061 gttttagaaa ttttctctca tccaaaagaa gaattgacac aggatttcat caaaacagca
     3121 acaggaattg atgaggccct ggtgaaaatt taccagcagg atattgttaa gaatttgcct
     3181 gagaacagta tcttagttca attgaaatat gctggttcaa atacggatac ggcgattgtc
     3241 aatgatttgt ataaattcta tcaagtatct gccaatattc tttatggcaa tattgaaatt
     3301 ttagaccata cgccagtcgg tgagatggtg gtgatcttat caggtgaacc tggtcagttg
     3361 caccgtgcga ttgaagcagt gacggaggcg cgtgtggaag tgacgatttt gaaaggagcg
     3421 aactagatgt tagaatggat tcaaacgaat tttccagata tttataaatt gggctgggat
     3481 ggtcagacag gttggttaac acattttaat ttgaccctct atatgacctt tgtttccttt
     3541 gcttttggtg gatttatggg cttggtgtct ggtttgttct tggtcttgac aggtccacgt
     3601 ggggttattg ccaataagac tgcctattgg attttggaca aggtggcttc tatcttccgt
     3661 gccattcctt ttattatctt gttggcggct attgctcctt ttacgaaaat tattgttgga
     3721 aaaaccattg gtacagaggc ggctttggtg ccgctcgccc tttcagtctt tccgttcttt
     3781 gcccgtcagg ttgaagtggt cttatcagaa ttggaccgtg gtgtcattga ggcggctcag
     3841 gcttccggtg ctaccttctg ggatatagtc ctagtctatc tacgtgaggg tctgcctgac
     3901 ttgattcgtg tgacgacctt tgccttggtt tccttggtcg gctatacagc catggcagga
     3961 gctatcggag caggtggttt gggacaagtt gccctatcat acggctacct acggtataat
     4021 aatgatgtga cctttttagc aactcttttg attttagtta tcattttcgt aattcagttt
     4081 attggagatt tcttgacgag aaagattagt catagatagt tatttagctg tgttttttca
     4141 aattttggat agtccttgac ttatttgaag catttttata caataaattt aattaaataa
     4201 acactgccac cgggtagtca tgcaacagca ttttatgaac gtcgttaggt cttagaagat
     4261 gttcataggg tgctgatttt tctataaaag gagtgagaat atgtcacaat ttgttgttga
     4321 ttcatctttt tggagtctat ttccagatgc aaaaattgga gttctattat tgaaagatta
     4381 taaaacgccg gcgcaatcgc ctgatgaatt agtgaaatta ctggaagaaa gcaacggaat
     4441 tgcccagaaa tttcttacgg aagatacctt tagtgagaat gaggtcattc gtacatatcg
     4501 tcaagcctat caaaaattta aaacaaaaaa aggcgcgcgt tctagtatcg aagcactttt
     4561 gaaacgttca actagtgacc gtccagtcac aacaatttct ccattagtgg acatttataa
     4621 tgcggcgagt ttacgttttg gtcttccatg tggcgctgaa gatttagata catttgtagg
     4681 caatctccaa ctaacggtca ctgagggtgg ggatgaattc tatctcattg gtgatgaaac
     4741 caataatcca actttaccag gtgaagtttg ctataaggat gataaaggcg ccgtttgtcg
     4801 ctgtttcaat tggcgcgatg gtgagcgaac catgattacg gatgagacaa agaatgcgtt
     4861 cttggttatg gagttggtca attccgatag agtggaagat ttggaaaatg cccttgactt
     4921 catcagtcag caagcagaga ccttcttggg agtagtgcca gaaaaatatc tactagattg
     4981 tgataggcct ggaatagcct tgtaggattt gtcagtggga ttgtgttcgg atagatagtg
     5041 gtctttctag acaaatgctg atttttctgc tacaatacta agcatgaaag aaggttgttt
     5101 caaaagcctg tctagcgagc ttgggagagt gaaagccaag ccagtcttga aataattggc
     5161 gctttcccag tcgtgaccat gtccgactca atcagaatca tacaaatgaa gtaataaatt
     5221 agggtggaac cgcgttctca acgcccctat gtctgttgac aggggaagag aatgtggttc
     5281 tttttgtctg ccgaccgagt atttcttgtt tttcatctca aggtgaaaaa cagttactat
     5341 aaagaaagga gaccaatgtt caccttagaa gctatcaatc acgggcatga gcagtttact
     5401 gggcctgatt ttcccaagct gattgcctat tttaaggact tgggtatggt ggaaaatatt
     5461 gtggatatcc agtctggaca ggttacctat cgctcacaga cagggcagac tctggaaaag
     5521 acgggctatc aagtaacgat acctgtttcg gaccaagcca atctggacca gtttgtgact
     5581 atcttacgca atcatcaggc tggacaaaca gattttccca ccttctgtca agaaacagca
     5641 gaagcaggcg tttacaagtg ggtcatcgac ctagaagcca tgacctgttc ctatctggac
     5701 caggcggagc aagctgtttt tgtggaagct gtaccgagtg tggagaacta gcaattttga
     5761 aatataaata gaaagaggat gttatgtcaa aaaaacttac ttttcacatc gtcagtggca
     5821 tagtctcctt tcagtcgaat tgccaaacgc tcatcgctat ggcgactgag ctagacgttg
     5881 tactagttcg cctttcctag cggtatcggc taggaaggct cacgtcgcaa tcaatttata
     5941 tagaaagagg aaatatatgt ctaaaaaatt aacattccag gaaatcatcc tgaccttaca
     6001 acaattttgg aatgagcagg gctgcttgct catgcaggct tatgatacgg aaaaaggtgc
     6061 ggggactatg agtccataca ctttcttgcg tgctatcggt cctgagcctt ggaatgcggc
     6121 ttatgttgag ccttcgcgtc gtcctgcgga tggtcgttac ggggaaaacc caaaccgtct
     6181 ctatcaacac catcaattcc aagtggttat gaaaccatct ccatctaaca tccaagaact
     6241 ctacttggaa tctttggagc gtttggggat taatcctttg gagcatgata ttcgtttcgt
     6301 tgaggacaac tgggaaaacc cgtcaacagg ttctgctggt ctgggctggg aagtttggtt
     6361 ggacggtatg gaaatcacac agtttaccta cttccaacag gttggtggtt tggcgacagg
     6421 tccagtcaca gccgaagtaa cttatggttt ggaacgtttg gcatcctata tccaagaagt
     6481 tgactccgtt tatgatattg agtgggctga cggtgtcaaa tacggcgaga tctttatcca
     6541 gccagaatat gagcattcaa aatactcctt tgaagtatcg gaccaagata tgctccttga
     6601 aaatttcact aagtttgaaa aagaagcaga gcgggctttg gaagagggct tggttcaccc
     6661 agcctttgac tatgttctca agtgctctca cacctttaac ctgctcgatg cccgtggagc
     6721 cgtatctgta accgagcgtg caggctacat tgcccgtatc cgtaaccttg cccgtgtcgt
     6781 tgccaagacc tttgtggcag agcggaaaaa actcggtttc ccacttttgg atgaagcaac
     6841 acgagcagaa ttgttgaagg aggacgcaga ataatgacaa agaatttact tgttgaactt
     6901 ggtttggaag aaattcctgc ctacatcgta accccatcca tgcaccagtt gcgtgaccgc
     6961 atggcgactt ttttgacaga caatcgcttg gcttttgata gcattgatgt cttttcaacg
     7021 ccacgccgtt tggctgttcg tgtccgtggt ttagcagacc agcagactga tcttacagaa
     7081 gatttcaagg gtcctgctaa gaagattgcc ttggatgcag acgggaactt cactaaagct
     7141 gcagaaggct ttgtccgtgg caaaggcttg acgacagcgg acatcgaatt ccgcgagatc
     7201 aagggtgaag agtatgtcta cgtgaccaaa cacgaagctg gtcagccagc taaggcagtc
     7261 ttggcaggca ttccagaggt cttgaaggct atgaccttcc ctgtcagcat gaactgggcg
     7321 tccaacaaat tcgcctacat ccgtcctgtc cacaccttga ccgttctttt ggacgatgaa
     7381 gcattggata tggacttctt ggacattaca tctggtcgca tcagccgtgg tcaccgtttc
     7441 cttggaaatg aaactgagat tgcaagtgca gattcttacg aggcagactt gcgtgcacag
     7501 tttgttatca ctgaccccgc agagcgtcaa aatatgatcg tcgagcaaat caaggctatc
     7561 gaggacaaac acaacgtgac agttgaaatt gatgaagatt tgctcaacga agttctcaat
     7621 ctggtcgagt acccaactgc cttcatgggt tcttttgaca ctaaatattt ggaagtacca
     7681 gaagaagttt tggtaacttc catgaaaaac caccagcgtt actttgtagt gcgggataag
     7741 gctggcaagc ttttgccaaa cttcatctcc gtccgaaacg gtaatgacca gtaccttgac
     7801 aatgtcatta aaggaaacga aaaagtcttg gtggcccgtt tggaagatgg tgagttcttc
     7861 tggcgtgagg accaaaaact caagattgct gacttggtag agcgactcaa agttgtgacc
     7921 ttccatgaga aaatcggctc actttacgag cacatggaac gcaccaaagt tatcgcagaa
     7981 aaactggctg acttggcagg tttgtctgcg ggtgaaaaag cagatgtggc gcgtgcggcg
     8041 gacatctaca aatttgacct cttgacaggc atggtcggtg agtttgatga attgcaaggg
     8101 attatgggtg agaagtatgc ccttcttgca ggggaaaaac ctgcggtggc agcggcaatc
     8161 cgtgagcact acttgccaaa ctcagcagaa ggcgaattgc ctgagagcaa ggtcggtgcg
     8221 gtcctggcac tggctgataa gtttgacacc ctcctatcct tcttctcagt tggcttgatt
     8281 ccgtctggtt caaacgaccc ttacgctctt cgtcgtgcaa cacagggtat cgtgcggatt
     8341 ttggaagcat ttggttggga aattccattg gatcagttga ttgcggagct ctacagcttg
     8401 aattttgcta gcttgaccta tgacaaccag ccagctgtta tggactttat ccgtgctcgt
     8461 gtagagaaga tgatggacaa gaccattccg aaagacatcc gcgaggctgt acttgccagc
     8521 tcaacttttg tggtcagact gcaactggca gccagctctg ctattttcca aaaagcaaaa
     8581 gaggctgact acaaggaagc cgtggaaaac ttgtcacgtg tcttcaactt ggctgaaaag
     8641 gcggaggtga ctgcgattga cgaggccctc ttcgaaaacg accaggaaaa agcccttgct
     8701 gctgcagtgg caggcttgga gttgacagaa gatatggcgg gcaacttgga caagctcttt
     8761 gctctcagcc cagtcatcgc ggccttcttc gacaatacca tggttatggt agatgacgca
     8821 gcagtcaaag ccaaccgctt gaccctcttg aaagccttgg cggataaggc aagtgctgtg
     8881 gcggtcttca atctcttgaa tagtaagtag gaggcattca tggaacaagc aaaaatcgat
     8941 cggatcaatg aactggcccg caagaaaaaa gcagaaggct tgacagagga ggagttgcag
     9001 gaacaggcgg ctcttcgtga ggagtacatc gagggctacc gccgtagtgt gcgtgcccac
     9061 atcgaaggta tcaaggtcgt cgatgaagac ggcaatgatg tgacgccaga gaaactccgt
     9121 caagtccaac gtgaaaaagg cttgcacgga cgtagcttgg acgatcccaa ttcctaagag
     9181 taagattgct aaaaaaacaa ggaaagacta ggttttctag tcttttttgt gcttttgaga
     9241 acatatattt acttgttcat tcgattcata aatggtaaaa tgtatataga taaataggct
     9301 caggagggct ttgtcatgaa aaaactactc tattttctct tgtttcttac ctacatcgca
     9361 tgtgcataca gcacgatttt ctttctgcct tttatgtact tttctggaat tgttggggca
     9421 acaggagttg gtctgggcct aagctattcc ctacatcaag aagtcaaaaa gcatcctgaa
     9481 aaacaattgt cagagtattt accgtgggga tctaagaggg acaagattat ggtagtaacc
     9541 attctacttt tctccatttt ggtcagtttc attccagatg tgaattggca tagaggcctg
     9601 gcaatcctct tagatatcag ctatgtactc ctttggcaag ctatgcttat ccaattctta
     9661 cgaaaatatt attttaaaaa cgcatgagag tttcatgcgt tttcttttct aaatactttg
     9721 tccagctttt aattttttca taaagatgat ggaacttgga atcaggacga tgatgcttcc
     9781 gatccaggcg gcggggttag cggcggcggt tccataaaaa ccgaaatact gcatgccgat
     9841 gatggcgact gctgctcgga aaatcagttc tccaaagcct gctagtgttg ggataaagcc
     9901 cttgccaagt ccctgaataa agctacgaag aataaagagg gaagcaacaa tccagtaaca
     9961 agagccgtta atgatgtagt aaacaagagc caagtccagc actgtttggc tggcatttgg
    10021 cagaaagaga ccagagaaga agcgattgcc caagatgaga atgaccgcaa atacaatggc
    10081 ccaagcaatg ctgaggagta atgagtggcg cactccttct cgaatacggt catagagctt
    10141 ggcaccgtag ttctgagcag taaaggttgc gatagccaag cccagattga ccatgggcag
    10201 catagccaac tggtcagtac gaagggcaat ggcctgggcg gcgatggcgt ctgttcctag
    10261 ttggttgacc ataaactgca aggtcatggc tccgatggcg ataatactgg cttgaaatcc
    10321 cattggaaaa gcaagctggg catgtttctt gagattacct ctatccaatt ttaaatcagc
    10381 tagtccgata tgatagtgag gtaccttgtg gataatgtaa aaaataagaa agaggaccga
    10441 acaggcttgg gcaaggatag ttgcaaagcc agctccgaaa acgccccagt tcataactaa
    10501 gataaagaaa aaatccagac cgatattgat aaaacaagcg ataactagag ccagtagagg
    10561 tgttttagag tttccgagac tgcgtagggc tgaagacagg aaattataaa aaatagtaaa
    10621 ggtcaatcct ccaaacatgg ccgttagaaa ggcatgggag tggtcgatga tactcacagg
    10681 cgtttgcatt aagaccaaga taggtttcaa aaaagctaag gctgagactg ttagtaaaag
    10741 gctgatgagg gaagcgtaga agagtccgtg gacaaaagat tttttaagcc cctctaagtc
    10801 gccagctcca aagcgttggg cagaaataat ggttaatcca ttggtaacac cttgggcaaa
    10861 accgatgatt aaaaaaatca ggctggctgt cgaaccgaca cttgcaaagg cgtcctttcc
    10921 caaagtatgc cccacaatca tggaatcagc aaaattatag gccagttgaa aaaaagatcc
    10981 aatcaacaag ggaatagcga attgtaagat gaccgcaatc ggctttcctt tggttaaatc
    11041 attcataacg tctccttttg acagatgata agcccactag ataaatgata gtctagtagg
    11101 ctttctaggg ataaaatatt tctcctgaaa acggagtata ggcgtaaatt tgttatttat
    11161 actcttcgaa aatcaaaatc agacgttgtt gacttgattt gatgagtgaa aggctccagt
    11221 ggagcctttc agcctgttac cttgaaacaa aatagtaaca gggttctatc tgcgtaagta
    11281 gtaacgaact acgttcgccc tatctccaac ctccaaaggt tccccaaacc tttggagcta
    11341 gtctgttttt gattttcatt gagtattaat ttgtcagttc ttcaaaatga tcgacagtgt
    11401 tatggtctgt tactgctaag atgagttcgt cagatttgaa gacatagtca ggagtcagtt
    11461 ggatgtttaa ttcctgattt tcgccacttc ggtaaccgat gatattgagt ttataatgtt
    11521 ggcggagttt taattcgccg agtgtttttc caatccattt tactggcggg tgaaactcga
    11581 caatggaaac attgccttcg agctcaaaga ggctggttgt atcgcggtgg agtagttgtt
    11641 ttgctagaga aatacctgtt tcacgttcag gagaaataac cttatcggca ccgacacgaa
    11701 gaaggacttc tttcgcagtt gttcctttga ctttgacaat gaccatagga acacctagaa
    11761 ctttgctatg catgacagcc agaacactgg attccaagtt ttcgccagta gctacaacaa
    11821 cagcatcgca gttaccgatt cctgctgcac gtaggagcga gcgatcggtg atgtctccga
    11881 caataccgcg tgtgagaata ggttctagat gattgatacg ttgctcatga ttatcgatag
    11941 caatgatatt gcaatcgtaa ttgtgtaatg tttttgcaat ggtagtacca aaaactccca
    12001 ggccgagaat tccgatagta tagtttggca tgatttctcc ttttaaacaa gcatagatga
    12061 ttttgcgtat tgaagacttt cggttttaga taattttcga acagaaaggc ttgcgagaat
    12121 agaaagtgga ccgatacggc cgaagaacat gagcgccata atgactgctt gaccggccat
    12181 ggttaggctt ggagtcagat ttgccgtaac acctacagtg gctaatgctg acatgacctc
    12241 gaacattaaa aatagaagtg gttgtcggtc atctgttagg gcaagtgcga agagccctat
    12301 gataaacata agtaggaaga ctgagaatgt cgcaaaagct ttgcggatgc tgaggttatc
    12361 gatggttcgt cccataaagt tggtatgagg gagaccgaga atctcgctac gagcatagag
    12421 cactaaggtt aggaaggcgg ttattttaat accgccggct gttccacctg gggctccgcc
    12481 aagcatcatc tgaaagatat agagcaagag ggtgattggt tcagccttcg tatagtcgat
    12541 gctggcaaaa ccagcggttc tcatggtgac cgtctggaaa aagctggcta ataatttatg
    12601 gccaaatgat agttgcccga tagtagaagg gttattccat tcagtgagta aggttgtcag
    12661 agttccgagg gttagtataa tggctgttag gcctagaaca atctttgtat ggaaccctag
    12721 ttttcgtagg ctacgttttt gcccgatttg tgtttgaagg tcaaaccaaa ccgagaatcc
    12781 aagacctccc ataataatga gactggctaa tgttatgttg attagaggat tatccacata
    12841 gcgcatgatg ctagttgcgc caaaattatc aaaaccagca ttacaaaagg ctgatatagc
    12901 tacgaaaatg gaggtaaata aacctctcgc ccatccaaat tcaggcacaa aatgaaagga
    12961 cagtaaaagg gctccaaagg cttcgaccgc caaggtaaaa ccaaaagctg atttgaggaa
    13021 gttacgaaaa tattttgtgt cagatcggtt tagagcttct tgtaaggtcg ccatattgat
    13081 gaaattcatt ttacgaccac cgcgtaaagc gaagagaccg atgaatccta tcaagctcaa
    13141 accaccaatt tggattagca tcatacagat aatctgtcca aaagtagaat aggtttcagc
    13201 aacggctttg gtaaatagcc ctgtcacaca aaccatggag acagatgtaa agagatgatc
    13261 ccaataactt gctgtagagg tgctaatttg tgagatggga agacttagta aaattgatcc
    13321 agtcagaatc acgaataaga aactgataat gattcgctga ctgggagaaa gtttgatcct
    13381 aaatggtgac ataattctac cttttctcaa aaaaataaaa gatgaagata ttgtagcaca
    13441 aaagaaagaa aatgtacgac taaaacacgc aaagaacctg atttttttct cttcttgaaa
    13501 gcttcattat tttttattac catggatttt ataagtggtg tttatatgag gtggctcatt
    13561 atatttgtga caccaggatt atggggaaat tattatgggc aatgaaaaag tggtgcggac
    13621 cataaaaaga atctccttag ttgacctagg gagatttatt tccattctgc taagactagt
    13681 tgatgatagt gacgtaaagg tccgaccttt tggaggaggt aggtagcagc cagtccgacc
    13741 aataagcctg ataaaattcc tgtaaaagcg aggattggca ggtagtttag tacatagaaa
    13801 gaacgggcaa tggtagcaaa aacaagaagc tgaccaagat tgtgcaacat cccgcccaga
    13861 atagaaattc caataggact gacacggttt ggaccgagct gcttggcaga gagcattcct
    13921 acataactca aagtagttcc ggcaaagcca tagaggaatg tggaaaaggt gcctcccaga
    13981 aaggttgaga tgagcaagcg tagagcgaca acctttaggc tatcctttgt agggagggta
    14041 aagatagcaa ttaagctaat caaatttccc agtcctagct tggcgcctgg agcaaaagca
    14101 aatggggggg tgatgagtcg ttcaatcaaa gaaatgacaa ctgcctgagc tgctaacatc
    14161 gtaataaagt taagtgtttg acgtttagaa ttcatgggtg tagagaaaaa actctttcta
    14221 gtcgattatc tattttacta tcatacttgt ttttgttcac tatggcaagt tttgtttgtg
    14281 tatattacta attattgaaa tttgcttgtc aactatcttg gaatacgata taatggaaac
    14341 atgtaatttg ttttgatttt cacaaggagg aaacattgtg acgaaaaata ttgtgattgt
    14401 cggtgctggt tatgcgggga ttgcagcagc tcgattgcta ggaaaaactt ttaaaaagga
    14461 tcaagatgta acagttacct tgattgataa gaactctttc catacctata tgacagagtt
    14521 gcatgaagtt gcggctgggc gtgtggaagc caatgcaatc aagtatgacc ttcaacgtat
    14581 cttcaaaaaa tatccaaagg ttcaattggt aacagacaag gttgttgaga ttgactacga
    14641 taagaaacaa gtagttgcgg aacaccaaac tctggatttt gattacctgc tccttgcaat
    14701 gggtggtgag gctaatgact ttggtgtgaa aggtgttaaa gaacacggtt ttacactttg
    14761 gtctatcgaa gcagcagaac gtttgcatga ccacatgatt gatgcttgct atcgtgccat
    14821 gcgtgagcat gatgaagcca aacgtcgcgc tcttttaacc tttaccgtta tcggtgctgg
    14881 cttcacaggt attgagatga ttggtgagtt gattgactgg gtaccaattt tggcgcgtga
    14941 gttcaagttg gatccaaaag aattctcact taaggttgtt gaggcaacgc caaatatctt
    15001 ggctatggtt actgaaaaag agcaagtcaa ggctcgcaaa taccttgaga aaaaaggtgt
    15061 tgaattggtt cttggcgatg gtgtagcaag tgtacaagaa gatagcttga cactttcatc
    15121 aggtcgtcaa attcctacct atacctctat ttggacagct ggtgttcaag ccaatacaga
    15181 cgcaagtgaa tttggtattg aaaaagcacg tgcaggtcgt ttggtagcca atgagttcat
    15241 ggaagcgaaa ggcaaggaaa atgtctatgt agctggcgac ttggtttact ttgaagaatc
    15301 agaaggaaaa ccaactccac agattgtaca agcagctgaa cagacaggcc atactgcggc
    15361 aagaaatatt atttcagcaa tcaagggtgg cgaaaaacat agctacaaag gtaaatatga
    15421 tgggtttatg gtatctattg gagcacgcta cggtgtagcc ttcttgatgg acaaatacca
    15481 catgtcaggc tttatggcaa tggctgtcaa acacatggta aacctactct acttctttac
    15541 tatccgtagt ttcttctaca tgggttctta tgttcgtcat gaattctttg acattcagaa
    15601 taagcgaaac atttttggcg gtcatacttc aggcaaagga aatcttctct ggtctgtgcc
    15661 aatgcgtgtc ctttatggtt cagtatggct ctacgaaggt atcaagaaag cctttggtct
    15721 atttggaaca acatcatggt ttggggatca agttgtcttc ccattccctt ggttagctga
    15781 tccagtatct ggtgcatctg ctgctgaggc ggtttcaagt gcctcacaag cggcaactgc
    15841 tgcggcagaa gcagctgaac caatttttgg tttgagctat gcctatggtg aagcaccgat
    15901 ggctgttttg gacaaaatgc ctgattggtt tgctactatc atggaattta tgatgccaaa
    15961 ccaagaagta gcactcttta tgcaaaaatt tatgacagta gcagaaatcg gtattggtct
    16021 ggctttgatt gctggtgcct ttgtatggat tgtatctgct gcaaccgttg ctttggtagt
    16081 tatgttcagc ctgtcaggca tgttctactg ggtaaatatt tggttcatcc cagcagctat
    16141 ttctcttatg aatggtgcgg gtagagcctt cggtctagat tactggatta tgccatggtt
    16201 gggtcgtttc ctagacaaga agatttatgg aaagccgaag catatttacc gtatcaagta
    16261 taaaaaataa aggaaatata gaaagttagt ggtacatcaa atttggaata cctatcctga
    16321 gattgagagg ggacttgaag aggtaaaatc catcatctta tctgagatga tggtcctgca
    16381 tccagcagtt acatcgaaga ttgttgaata tgtagaggcg ccagggaaat acctgcgtgc
    16441 tggcctctgt ctgctctttg cacaaatgac ggaaggaaag attagccgtt caaagctata
    16501 ttttgctgcc catttagaag tcctgcatct ggcgactctt attcatgacg atgtgattga
    16561 tggcgcggat aaacggcggg gagtcacggc ggctcatcaa caattttcga atcgaatcgc
    16621 tatctacacg ggtgattatc ttctggccta ttcaagtcga ctatttggaa aggggcttcg
    16681 tttgctagaa gtatcacgtg atgatttgga tttgggcaat gataagctga tggaaacaat
    16741 tcttcgaggg gaattatctc agctactcaa tcaatttgat gccaatatga ctatgaaagc
    16801 ctatctcaag caaattcaag gaaaaacagc ttttctattt ggacttgcct gtcagttggg
    16861 gagctttgta ccaggccaat ctaggaaaga cagtagcatg gcttttcggt ctgggcaagc
    16921 acttggcatg gcatttcagc tacgagatga cttaattgac tatcagctgg atgtagcaga
    16981 atcaggaaaa cctagattgc aagatattca aaacggtatc tatactgcac cgctactttt
    17041 tgccatgcga gaagacaagt ctatccagca gcaattgcgg gcttatattc aatcccctac
    17101 ggcagaaggg ttggaaatcc ttacagatag gatttttgca acgcatgcta ttgccaaaac
    17161 agagaaactg attgttagct atttggaaaa aatgatgaat ttt
//
LOCUS       DBNFEG010000027        17046 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.027, whole genome shotgun sequence.
ACCESSION   DBNFEG010000027 DBNFEG010000000
VERSION     DBNFEG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 17046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17046
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.027"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(312..794)
                     /locus_tag="AC1PNG_000619"
     CDS             complement(312..794)
                     /locus_tag="AC1PNG_000619"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HIP6701141.1"
                     /translation="MKKEVTVESFELDHTIVKAPYIRLISEEVGPKGDIITNFDIRLI
                     QPNENAMDTAGLHTIEHLLAKLIRQRIDGLIDCSPFGCRTGFHMIMWGKQDSEKIAQV
                     IKSSLEEIAEGITWEDVPGTTIESCGNYKDHSLHSAKEWAKLILSQGISTDAFERKPI
                     "
     gene            922..2535
                     /locus_tag="AC1PNG_000620"
     CDS             922..2535
                     /locus_tag="AC1PNG_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIP6701142.1"
                     /translation="MEIITVVVTLVSALIGLVLGYFAISLKMKSAKETAELTLLNAEQ
                     EASNVRGRAEEQAEVILKTAERDRQTLKKELLLEAKGEARKYREEIAEEFKSERQELK
                     QIESRLTERATSLDRKDDNLTNKEKTLEQKEQSLTDKAKHIDEREQEVVKLEEQKALE
                     LERVAALSQEEAKEIILTDTRDKLTHEIATRIKEAEREVKERSDKMAKDLLAQAMQRI
                     SGEYVAEQTITSVHLPDDAMKGRIIGREGRNIRTFESLTGIDVIIDDTPEVVVLSGFD
                     PIRREIARMTMETLLQDGRIHPARIEELVEKHRVEMDNRIREYGEAAAYEVGAPNLHP
                     DLIKIMGRLHFRTSYGQNVLRHSIEVAKLAGVLAAELGENVNLARRAGFLHDVGKAID
                     REVDGSHVEIGTELAKKYKENPIVINAIASHHGDVEATSTIAVIVAAADALSAARPGS
                     RRESMEAYIKRLQDLEEIANSFEGIKQSYAIQAGREIRIIVHPNKVTDDQITILAHDV
                     REKIENNLDYPGNIKITVIRETRATDVAK"
     gene            2683..3309
                     /gene="gmk"
                     /locus_tag="AC1PNG_000621"
     CDS             2683..3309
                     /gene="gmk"
                     /locus_tag="AC1PNG_000621"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIP6701143.1"
                     /translation="MKERGLLIVFSGPSGVGKGTVRKEIFESSDNKFEYSVSMTTRPQ
                     RPGEVDGVDYFFRSREEFEDLIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDGVFIFLTPPDLEELQDRLIGRGTDSEEVIAQRIERAKEEIALM
                     REYDYAVVNDEVPLAAERVKRIIEAEHFRVDRVIGRYDEMIREQIAER"
     gene            3333..3647
                     /gene="rpoZ"
                     /locus_tag="AC1PNG_000622"
     CDS             3333..3647
                     /gene="rpoZ"
                     /locus_tag="AC1PNG_000622"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIP6701144.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAKPTQEFTSV
                     KSTLRALEEIESGNVVIHPDPEAKREAVRRRAEEAKRLAEEEERKIKAQIAKEKEEGE
                     KI"
     gene            3991..6381
                     /locus_tag="AC1PNG_000623"
     CDS             3991..6381
                     /locus_tag="AC1PNG_000623"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HIP6701145.1"
                     /translation="MLAQIIVDVPLMQTDQAYSYRVPAELEEGLKVGVRVHVPFGKGN
                     RLIQGIVVDLIDEDDENNLQDLKELAEVLDYSPVLNQEQFWLADQMRKSVFSYKIALL
                     KSMLPSLLNSTYDKLLRPGLGLEMAEQISLFGDKEQIRFSDLDVTEQGKIMRLAQSGK
                     ILVDYVAKDKKQIKTEKWYRVCLEKLREADISNRAKKRQQLREFLLEHSEDQLLSNLK
                     SAYSADTLRYFQEKGYIEVWEEEVSRTQGVFDKVEKTQALDLNPEQAIAVREIVASIG
                     QESQTFLLQGVTGSGKTEVYLQVIDRVLKMGKTAIMLVPEISLTPQMTNRFISRFGQQ
                     VAILHSGLSDGEKYDEWRKIEAGNAQVVVGARSAIFAPLTNLGVIIIDEEHEATYKQD
                     SNPRYHARDVAKLRADYNRATLVLGSATPSLETRARASRGVYGRLTLNQRANPLARIP
                     EVEVVDFRDYIGQQEASNFTPVLIEKIREKLARKEQVVLMLNRRGYSSFVMCRDCGSV
                     DQCPNCDISLTLHMDTKTMNCHYCGFQKGIPQTCPNCQSRSIRYYGTGTQKAYDELQE
                     LLPEARILRMDVDTTKKKGAHEDLLERFGRGEADILLGTQMIAKGLDFPNVTLVGVLN
                     ADTALNLPDFRSSERTFQLLTQVAGRAGRADKEGEVLIQTYNPNHYAIAFAKEQDYEG
                     FYRYEMGIRKNLGYPPYYFTVGLTFSHKSEEFVVKKAYETVAFLRQHLTDAIQILGPT
                     PKPIARTHNLYHYQIILKYRHEDRLEEVLNQILDWTQERENQDLRLIIDNEPQNMM"
     gene            6512..7450
                     /gene="fmt"
                     /locus_tag="AC1PNG_000624"
     CDS             6512..7450
                     /gene="fmt"
                     /locus_tag="AC1PNG_000624"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIP6701146.1"
                     /translation="MTKLIFMGTPAFSAMVLRGLLADGNYNILAVVTQPDRAVGRKKV
                     IQMTPVKEVALEYNLPVYQPEKLSGSQEMDELMNLGADGIVTAAFGQFLPTKLLNSVD
                     FAVNVHASLLPKYRGGAPIHYALINGDERAGVTIMEMVKEMDAGDMISSDSIAIEESD
                     NVGTLFEKLAVVGRDLLLQTLPAYIAGDLKPVAQNPEQVTFSPNIQPEEEVLDWNKTA
                     RQLFNHIRGMYPWPVAHTYWQGERFKIQEAVEAEGEGPVGRVIARSKKELIIATGQGA
                     LSLKTVQPAGKPKMTIADFLNGAGRDIAVGDQFGDQ"
     gene            7437..8750
                     /gene="rsmB"
                     /locus_tag="AC1PNG_000625"
     CDS             7437..8750
                     /gene="rsmB"
                     /locus_tag="AC1PNG_000625"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIP6701147.1"
                     /translation="MVINKHETARSLALSVLEQVFDQGAYSNIALNKALESSRLSAQD
                     KGLATELVYGTVSRKITLEWYLAHFIEDREKLDTWVYYLLMLSLYQLVYLDKLPTHAV
                     VNEAVNIAKRKPGTDKFVNAILRKLSQDSLPKPADIKRKNKRLSVQYSVPVWLVQTLI
                     AEYGDERAEKIFQSLHDRNKASVRVTDGQQVEQLAEELGAQHSQLSPVGLVKSHGHFA
                     GTDYFKKGLITIQDETSQLVAPTLGIQGDEIILDACAAPGGKTCHMASYLTDGKVYAL
                     DLYDHKLNLIEENARRLGLEDKIETKGLDASRVHEEFGPDTFDKILVDAPCSGIGLIR
                     RKPDIRYNKASMDFDSLKTIQLQILDSVCQSLKIGGIITYSTCTIIAKENQEVIQEFL
                     QNHPNFEQVTLEHPQTDIMVDGCLLITPEQYKTNGFFIGQLRRKS"
     gene            8788..9525
                     /locus_tag="AC1PNG_000626"
     CDS             8788..9525
                     /locus_tag="AC1PNG_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIP6701148.1"
                     /translation="MEIALLTDVGQKRSNNQDYINRYKNRAGIDLVVLADGMGGHRAG
                     HIASEMAATDLGAAWVDTQLVTLNDVREWMVAIIDAENQKIHELGQTEEYKGMGTTLE
                     AVVVIDNQMIYAHVGDSRVGLIRDGEYTRLTNDHSLVGALVRAGQLTEEEAQRHPQKN
                     IVTQSIGQAEPIEPDIALKTLEVGDYVLINSDGLTNMIPTEDIRDIVLSDVSIESKVE
                     TLVRFANNAGGLDNITVGLLHITEEAR"
     gene            9525..11054
                     /gene="pknB"
                     /locus_tag="AC1PNG_000627"
                     /pseudo
     CDS             9525..11054
                     /gene="pknB"
                     /locus_tag="AC1PNG_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893497.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
     gene            11031..11519
                     /locus_tag="AC1PNG_000628"
     CDS             11031..11519
                     /locus_tag="AC1PNG_000628"
                     /inference="COORDINATES: protein motif:HMM:NF015734.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIP6701149.1"
                     /translation="MAPQVVPNVVGKSQENATQILQTAGFSIGTITQEYSSSVTAGQV
                     ISTDPVANTELAKGSIINLVISKGKELIMPDLTSGNYTYSQARSQLQALGVNAESIEK
                     QEDRSYYSTTSDIVIGQYPAAGATIDGTVTLYVSVASTRTSSDSSAGSSTSTSTSTGS
                     GQ"
     gene            11940..12635
                     /gene="liaF"
                     /locus_tag="AC1PNG_000629"
     CDS             11940..12635
                     /gene="liaF"
                     /locus_tag="AC1PNG_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HIP6701150.1"
                     /translation="MRKVQFFLLIESMIFTMAAFDVVANEASRTLLLFSALLLVIWYF
                     LGRKVNSVLLVSSLSLLFSVFVLNPFFIIGIMLLVVYMLINFFSRYEKRNQYTHIVFS
                     DYSLQVQKEKNRWFGNHDHSQDRFGFEDINIVRLFGNDVVDLDKTVLVGRDNIVVIRK
                     TFGRTKIIVPIDVEVALSATSIYGRVSFLGESYWDLRNESIAVNSSDYQESHKRVKVV
                     TNNILGDVEVVRV"
     gene            12632..13648
                     /locus_tag="AC1PNG_000630"
     CDS             12632..13648
                     /locus_tag="AC1PNG_000630"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIP6701151.1"
                     /translation="MRKRTYLLLAWFACLIVFVVIASTLPLLNYSLLDVTLWISTEQL
                     VFTLILLVIVLTLFLAVMVQTVSLASTQVMRTKLQAILKNKSIRTDTESDQLLLQLSD
                     KVRNLTRQVQLVDNQDLVKKEEIVEGERKRIARDLHDTVSQELFATSMILSGLSSNLS
                     TLPQETLQEQLMLVKDMIESAQRDLRILLLHLRPSELESKTLVEGFELILREVSDKSN
                     IIVHFQHEVEELPKLIEEHLFRIAQEIISNTLRHAKAKHLDVYLLQKETELQLKMTDD
                     GIGFQQGADDELSYGLQNIRERVEDMAGTIKIRTAPNKGVAIDIRIPLLKGKEDEYDS
                     SDAG"
     gene            13620..14261
                     /locus_tag="AC1PNG_000631"
     CDS             13620..14261
                     /locus_tag="AC1PNG_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIP6701152.1"
                     /translation="MNTIRVMLVDDHEMVRLGLKSYLNLQPDVEVVAEASDGEEGLAK
                     ALEVKPDVVVMDLVMPKMTGVEATLALLKEWPQAQIVILTSYLDNEKIYPVLEAGARG
                     YMLKTSSADEILAAIRKVALGEYAIETEVEKKVEHHKRHPDLHDDLTAREREILTLLA
                     KGYDNQRIADESFISLKTVKTHVSNILSKLAVSDRTQAVVYAFQHGLVAQEDQ"
     gene            14311..15711
                     /locus_tag="AC1PNG_000632"
     CDS             14311..15711
                     /locus_tag="AC1PNG_000632"
                     /EC_number="3.1.3.-"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HIP6701153.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRVKNTGYIPESIQQVFNSLKE
                     KGILTGIASGRTPYGLVPEIKALKPDFFAMINGSYVEDAKGQVVYHQPMPQNIVESVL
                     NWAKEIGIEYGMLGSQKGTLSARTDRISQVIDLIYEGLEINPTFYKENDIYQLLTFEK
                     DGHEVELPEELQAELRSVRWDAISSDIVLKGSSKATGVAKVVEKLGLKPENVLVFGDG
                     LNDIELFDYAGISIAMGHSHPELQKHADYITKKVEEDGIFDALEKLGMVEKEKYFPQL
                     DLENVTGPVAHIKTNHGKLTVKLFPEIAPKTVANFVALSKDGYYDGIIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGTAFEDEFSMEAFNLRGALSMANAGPNTNGSQFFIVQNQNFP
                     YNAKELERGGWPKEVAEAYVKNGGTPHLDQRHTVFGHLVDEESFVVLDAIAAVATDSA
                     DRPHEDVVIETIEIED"
     gene            15713..16084
                     /locus_tag="AC1PNG_000633"
     CDS             15713..16084
                     /locus_tag="AC1PNG_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIP6701154.1"
                     /translation="MKIGDKLKGTVTGIQPYGAFVQLENGTTGLIHISEIKTGFVDNI
                     YDHLKLGQEVLVQVVDFDEYTEKASLSIRTLEEEQQKIPRHHRFTNERNKIGFSPLAK
                     QLPKWIEEAKGYLKEHKEKKV"
     gene            complement(16111..17037)
                     /gene="cysK"
                     /locus_tag="AC1PNG_000634"
     CDS             complement(16111..17037)
                     /gene="cysK"
                     /locus_tag="AC1PNG_000634"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIP6701155.1"
                     /translation="MAIYQNITQLVGKTPVIKLNNIVPEGAAEVYVKLEAFNPGSSVK
                     DRIALAMIEDAEKAGTIKPGDTIVEPTSGNTGIGLAWVGAAKGYKVIIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAKEIAEEKNGWVPFQFANPSNPKVHEDTTGQE
                     ILEDFGTTGLDAFVSGVGTGGTVSGVSHVLKTANPDIAIYAVEADESAVLSGEAPGPH
                     KIQGISAGFIPDTLDTSAYDGIIRVKSDDALATGRAIGGKEGFLVGISSGAAIHAAIE
                     VAKELGTGKKVLAILPDNGERYLSTALYEFND"
BASE COUNT         5145 a         3195 c         3966 g         4740 t
ORIGIN      
        1 taaaccagat gggacctact tgttctctat aaattgtaag acaggctact ttgtcggcaa
       61 tggtgctggt cattctgatg ccttggatta ttaagcaaaa aagcgtttta gccgcttcag
      121 attgaagaaa aagtccattt tggacaattt tcttcaatct tttttcttta tgttgaaaat
      181 aaaaaactct cagaaacgcc gaaatatcaa tgtttctaag agtttaatga cttgctatct
      241 ttcgtaaact tcttcaattt tttatatttt aggattttgt ctacgttctg agcggtgtat
      301 ccgttttttt gttagattgg ttttctttca aaggcatctg tagagattcc ctgagaaagg
      361 attagtttgg cccattcttt agctgaatga aggctgtggt ctttgtagtt accgcatgat
      421 tcaatggttg ttcctgggac atcctcccaa gtgatgcctt ctgcaatttc ttccaaactc
      481 gacttgatga cttgagcaat cttttcagag tcttgttttc cccacataat catgtggaaa
      541 cctgtccgac acccgaaagg agagcagtcg attagcccat cgatgcgctg gcgaattagt
      601 ttagctaata gatgctcaat ggtatgtaga ccagctgtat ccattgcatt ttcgttgggt
      661 tggataaggc gaatatcaaa gtttgtaata atgtctcctt ttggtccgac ttcttcagaa
      721 attaagcgga tgtaaggtgc ttttacaata gtgtggtcta gctcgaagct ttcgacagtg
      781 acttcttttt tcatggtttc ctccaaataa gtgatgaatt tattatacca tatttgacaa
      841 tttggaattg tgaaatcgtt tcaaatatga taaaatagat acagattttg tgaaaatcga
      901 attataaaaa agaggtagaa tatggaaatc attacagttg tagtgactct tgtttctgct
      961 ctcattggtt tagtattagg ttactttgcc atttctttga agatgaaatc tgcaaaagaa
     1021 actgcagaat tgacactttt aaacgcagaa caagaagcta gtaatgttcg tggtcgagca
     1081 gaagagcaag ctgaggttat tctaaaaaca gctgagcgcg atcgccagac cttgaaaaaa
     1141 gaattactct tggaagctaa gggagaagct cgtaagtacc gagaagaaat tgcagaagaa
     1201 tttaagtcag aacgtcaaga gttgaagcaa atcgaatcac gcttgacaga gcgtgcgacc
     1261 agcttggacc gcaaagacga taatttgacc aacaaggaaa agactttgga acaaaaggaa
     1321 caaagtctga ccgataaagc taagcacatt gatgagcgtg aacaagaagt tgtaaaactc
     1381 gaagaacaaa aagctttgga gttagaacga gtggcggccc tcagtcaaga agaggcgaag
     1441 gaaattatcc ttaccgatac acgcgataaa ctgacgcatg aaattgcgac tcgtatcaag
     1501 gaagcagagc gtgaagtaaa agagcgttct gacaagatgg caaaggactt gttagcccaa
     1561 gcgatgcaac gcatatcagg tgaatatgtg gctgagcaga cgattacatc ggttcattta
     1621 ccggatgatg cgatgaaggg acggattatt ggacgtgagg gtcgaaatat tcgtaccttt
     1681 gaaagtctca caggtatcga tgttatcatt gatgatacac cagaagtggt tgttctatca
     1741 ggttttgatc cgattcgccg tgagattgcc cgtatgacca tggagacctt gcttcaagat
     1801 ggtcgtattc atccagctcg tattgaggag ttggttgaga aacaccgtgt agagatggat
     1861 aaccgtattc gtgagtatgg ggaagctgct gcttatgaag ttggggcacc aaaccttcat
     1921 ccagacttga ttaaaatcat gggacgtttg catttccgta cctcttatgg acaaaatgtt
     1981 cttcgccact caatcgaagt tgctaaatta gcaggtgtac ttgctgctga attgggcgag
     2041 aatgtgaatt tggcacggcg agctggtttc ctacatgacg ttggtaaagc tattgaccgt
     2101 gaggtggatg gaagccacgt tgaaatcggt acagaattgg ctaagaaata taaagagaat
     2161 ccaattgtta tcaatgcgat tgctagtcac catggcgatg tagaagcgac aagtactatt
     2221 gcagtcattg tggctgcggc agatgctctg agcgctgctc gtccaggttc gcgtcgtgaa
     2281 tctatggaag cctacatcaa acgtctgcaa gacttggaag aaatcgccaa cagttttgaa
     2341 gggattaaac aatcctatgc tattcaggct ggtcgtgaaa ttcggattat cgttcatcct
     2401 aataaggtca ctgatgatca gattaccatc ttggcccatg atgttcgtga gaaaattgaa
     2461 aataatctgg attatccagg aaatatcaaa atcacagtta tccgtgaaac aagagcgaca
     2521 gatgttgcta agtaaatgta gaagagtccg aagggctctt tttctactgg ctcaaagttc
     2581 gtttagggtt gggaatagaa aatattctaa tcgtatttaa aagcagttga aattcatgct
     2641 aaattttgtt acactagaat gaaagattta aaaggagata tcatgaaaga gcgaggctta
     2701 ctcattgtct tttctggccc atctggtgtt ggaaaaggaa cagttcgaaa ggaaattttt
     2761 gaaagctctg ataataaatt tgaatattct gtatccatga cgacacgtcc acagagaccg
     2821 ggagaagttg atggagttga ctatttcttc cgtagtcgtg aggaatttga agatttgatt
     2881 cgccagggtc aaatgttgga atatgcagag tacgttggaa attactatgg aacacctctg
     2941 acctatgtca atgaaactct tgataaggga attgatgtct ttttagaaat tgaagtacag
     3001 ggtgccctcc aagtgaagaa aaaggttcca gatggggtgt tcattttctt gacaccacct
     3061 gacttggagg agttgcaaga tcgcttgatt ggtcgcggaa cggatagtga ggaagttatt
     3121 gctcaacgta tcgaacgtgc caaggaagag atcgccttga tgcgtgagta tgattatgca
     3181 gttgtgaacg atgaagtccc gttggctgct gagcgtgtca agcgtattat cgaagcagaa
     3241 catttccgtg tagaccgagt gattggtcgc tacgatgaaa tgattcgtga acagatagca
     3301 gaacgataaa agaaagaaga gagaatcgca ttatgatgtt gaagccgtca attgatacct
     3361 tgttggataa ggtaccatct aagtattctt tggttattct tgaggctaag cgtgcccatg
     3421 aattggaagc aggtgccaaa ccaactcaag aattcacctc agtgaaatcc accctacgcg
     3481 ccttggaaga aattgaatca ggtaatgtag tcattcaccc agacccagaa gcgaaacgtg
     3541 aggctgttcg tcgccgtgcc gaagaggcta aacgtttggc ggaagaagaa gagcgtaaaa
     3601 tcaaagctca aattgctaaa gaaaaagaag aaggcgaaaa gatttagaaa caagactcgg
     3661 ttgggattgc catccgggtt tcttttcact ttcggaatga tgaaagtttt gacagaaagg
     3721 aggcaggtca tttagtttat cttttaatat ttggggagtg ggaaagaact cgaccaagat
     3781 aaaaagagtt cgtcttccca cccccgcaca gttgattagg ctagatttgg agtgcaaaac
     3841 acgaacaaat ctgccaatca accactgcgc tgagatgttg acacgaactc tgagtagtgg
     3901 tcctgggctt tttgcccagc ctcgttatac ataaagtgaa ccttgttcgc atcagtcgtg
     3961 atattagaag gtaaaataaa tggagatggt atgttagcac agattattgt agacgtccct
     4021 ttgatgcaga cagatcaagc ttattcctat cgagttccag ctgagttgga agaggggctc
     4081 aaggttggcg tgcgtgtcca tgtacccttt ggtaagggaa atcggctgat tcaaggtatt
     4141 gtagttgatt tgattgatga agatgatgaa aataacttac aagacttgaa agagcttgcc
     4201 gaggttttgg actatagtcc tgttctcaat caagagcaat tttggttggc ggaccagatg
     4261 agaaagtcgg tcttctccta taaaattgcc ctcttaaaat ccatgttgcc tagcttgctc
     4321 aattcgacct atgacaaatt gctccgtcca gggcttggtt tggaaatggc agagcagatt
     4381 agtctgtttg gtgataagga gcagattcgt ttttctgact tggacgtgac agagcaaggg
     4441 aaaatcatgc gtctggccca gtcaggaaaa atcttggtag actatgtggc caaggataag
     4501 aagcaaatca agacagaaaa atggtaccga gtttgtctgg agaaattacg agaagcagac
     4561 atcagcaatc gagccaagaa acggcagcag ttaagggaat ttcttctgga acactcggaa
     4621 gaccagcttc tttcaaacct aaaatcggcc tattcagctg atacgctccg ctatttccaa
     4681 gaaaaaggct atatagaagt ctgggaggag gaagtgtcgc ggactcaggg agtttttgat
     4741 aaggtggaaa aaacgcaggc cttggatttg aatccagagc aggcgattgc ggttcgtgag
     4801 attgtggcct ctatcggtca ggaaagtcag acatttctac tgcaaggagt gacaggttct
     4861 gggaagacgg aagtctatct ccaggttatt gatagggtct tgaaaatggg aaaaacggct
     4921 attatgctag tgcctgaaat ttccttgacc cctcagatga ccaatcgttt tatttctcgt
     4981 tttggccagc aggttgccat tttacactcg ggcttgtctg atggtgagaa gtatgacgag
     5041 tggcggaaga ttgaggcggg gaatgcccag gtggttgtcg gggctcgttc ggctattttt
     5101 gcaccattga caaacttggg tgttatcatc attgatgagg agcatgaggc gacctacaag
     5161 caggacagca atccacgcta ccatgcgcgt gatgtggcaa agttgcgggc tgattataat
     5221 cgagcgaccc tggtcttggg ttctgctacg ccgagtcttg aaacacgggc tcgggctagt
     5281 cgaggagtct atggaagact gactcttaac cagagggcca atcccttggc tcgtattcca
     5341 gaagtggaag ttgtggattt ccgtgattat atcggtcagc aggaggctag taattttacc
     5401 cctgttctga ttgaaaagat aagagaaaaa ttggctcgta aggaacaggt cgttcttatg
     5461 ttgaataggc gtggttattc ttcttttgtc atgtgtcgtg attgcgggag tgttgaccag
     5521 tgtcccaact gtgatatttc ccttacgctt cacatggata caaaaacgat gaattgccat
     5581 tattgtggtt ttcaaaaggg gattcctcag acctgtccaa actgtcaaag tcgttctatt
     5641 cgttattatg ggacgggtac gcaaaaggcc tacgatgaat tgcaggagct cttgccagaa
     5701 gctagaattt tgcgtatgga tgtggatact accaagaaaa aaggtgctca tgaggacttg
     5761 ctcgaacggt ttggtcgagg cgaagcggat attcttctgg gaactcagat gattgccaag
     5821 ggcttggatt ttccaaatgt gaccctagtt ggcgtgctca atgcggatac ggctctgaat
     5881 ttgcctgatt ttcggtcttc tgagcggacc ttccagttgc taactcaggt agctggtcgg
     5941 gctggtcgag ctgataagga aggggaggtc ctgattcaga cctataaccc caatcattat
     6001 gccattgctt ttgccaaaga gcaggattat gaaggtttct atcgttatga gatgggcatt
     6061 cggaaaaatc ttggttatcc accttactat tttacagttg gcttaacctt ttcgcacaag
     6121 tcagaagaat ttgtcgtgaa aaaagcctat gaaactgtag cctttctccg tcaacactta
     6181 acagatgcaa ttcaaatctt gggaccgacg cccaagccta ttgccagaac tcataatctc
     6241 taccactacc agatcatctt gaaataccgg catgaagacc gtttggaaga ggtattgaat
     6301 cagattttgg attggacaca ggagagagaa aatcaagatt tgcgactgat tattgacaat
     6361 gaaccgcaga atatgatgtg aggtcactat tgaccttgca tttttttgtt ttaagaacct
     6421 ttgattaagg aaaaaataag caaaaatgat ataattaggg gatagtataa ttgatatgaa
     6481 tcagaaaatg aaaaggagat gagacacgaa tatgacaaag ttgattttta tgggaacgcc
     6541 agcgttttca gcgatggtct taagagggct cttagctgat ggaaattata acatactggc
     6601 agttgtaacc cagccagacc gagctgttgg acgaaaaaaa gtcatccaaa tgacacctgt
     6661 gaaagaagta gccttggaat acaatttacc agtctatcaa ccagagaagc tatctggaag
     6721 tcaagaaatg gatgagctga tgaatttagg agcagatggg attgtgacgg cagcttttgg
     6781 gcaattccta ccaaccaaat tattgaactc agttgacttt gcagtcaatg ttcatgcctc
     6841 tcttcttcct aaataccgtg gcggtgcccc tatccattac gctttgatta acggtgatga
     6901 acgtgctggc gtaaccatca tggaaatggt caaggaaatg gatgccggtg acatgatttc
     6961 cagcgatagc attgctattg aagaaagcga caacgtcggc accttgtttg aaaagttggc
     7021 tgttgttggt cgggatttat tattacaaac cttaccagcc tatattgctg gtgacttgaa
     7081 accagtcgct caaaatccag aacaggtaac cttctcacca aatatccagc cagaagaaga
     7141 ggtactggat tggaataaga cagctcgtca actatttaat cacattcgcg gtatgtaccc
     7201 gtggcccgtt gcccacacct attggcaagg ggaacggttt aaaattcaag aagctgtaga
     7261 agcagaaggt gaaggaccag ttggtcgggt tattgctcga agcaagaaag aattgatcat
     7321 tgcgacaggt cagggagccc tttctctgaa aactgtccag ccagctggta agccaaaaat
     7381 gaccattgca gactttttaa atggtgcagg tcgagatatt gcagtaggag atcaatttgg
     7441 tgatcaataa acatgaaaca gcaagaagtc tagccttatc tgtattggaa caagtctttg
     7501 atcaaggtgc ttactcgaac atcgctctca ataaggctct agaatcttct cgtttatcag
     7561 cacaggacaa gggcttggcg accgaattgg tttatgggac ggtttctcgt aaaattaccc
     7621 tagagtggta tttggcgcat tttatcgaag atagggaaaa gttggacact tgggtctact
     7681 atctcctcat gttgagcctc taccagttgg tatatttgga caaattgcct acccacgcag
     7741 ttgtcaatga ggcagttaat attgccaagc gaaagcctgg gacagataag tttgtcaacg
     7801 ctattttgcg taagcttagt caggattcgt tgccgaaacc agcagatatt aaaagaaaga
     7861 acaaacgtct atccgttcag tattctgtgc cagtttggtt ggtccaaacc ctgattgcag
     7921 aatatggcga tgaacgtgca gagaagattt tccaaagctt acatgatcgg aataaggcca
     7981 gcgtacgcgt gacagatggt caacaagtgg agcaattggc agaggagcta ggcgcccaac
     8041 actcacagtt atcaccagtt gggttggtta aatcccatgg acactttgcg gggacagatt
     8101 atttcaagaa aggacttatc accattcaag atgaaaccag tcaattggta gctccgactt
     8161 tgggtattca aggagatgaa atcatcttgg atgcctgtgc agctccgggt ggaaagacat
     8221 gtcatatggc aagttatctt acggatggaa aagtttatgc gttggattta tatgatcata
     8281 aactaaacct gattgaagaa aacgcacgtc gtttaggtct agaagataag attgagacca
     8341 aaggtctgga cgccagtcgt gttcatgagg aatttggtcc agataccttt gataaaatcc
     8401 tagtggacgc cccttgttca ggtattggac tcatccgccg taagccagat attcgttata
     8461 acaaggcatc gatggacttt gattctctaa agactatcca gttgcagata ctggacagtg
     8521 tttgccaaag cttgaaaatt ggtggtataa taacctatag cacatgtacg attattgcta
     8581 aggaaaacca agaggttatt caagaatttc tgcaaaacca tcccaatttt gaacaggtga
     8641 cactagaaca ccctcaaaca gatattatgg tggatggctg cttgttgata acccctgaac
     8701 agtataagac aaatggcttt ttcatcggtc aacttagacg aaaatcctag aataagagga
     8761 ggaagttgct tagcaacgat aaaaattatg gaaattgcat tacttactga tgtaggacag
     8821 aaacgttcga acaaccaaga ctatatcaat cgctacaaaa accgcgcagg gattgatttg
     8881 gtcgtgttag ctgatggaat gggtggtcac cgtgccggcc atattgctag tgagatggca
     8941 gcaacagatt tgggagctgc ttgggttgat actcaattag tgactttgaa tgatgtccgt
     9001 gaatggatgg ttgcaatcat tgatgctgaa aaccaaaaga ttcatgaact tggacagaca
     9061 gaagaataca aggggatggg aacaactctt gaggcagttg ttgtcatcga caatcaaatg
     9121 atctatgccc atgttggaga ttcacgagtt ggattgatta gagatggcga atatacacgt
     9181 ctaactaacg accattcgct ggtaggtgcc cttgttcgag ctggtcagct cacagaagaa
     9241 gaagcacagc gtcatcctca aaaaaatatt gtaacgcagt ccattggaca agccgaacca
     9301 attgaaccag acattgcttt gaaaactctt gaggttggag attatgtcct tattaacagc
     9361 gatggcttaa ccaatatgat accaaccgag gacattcgtg atattgtatt gagtgatgtt
     9421 tcaattgaaa gcaaggtaga aacacttgta cgttttgcca ataatgcagg cggtctagat
     9481 aatattacag taggtttgct tcacattacg gaggaggcta ggtaatgatt caaatcggta
     9541 agatctttgc cggtcggtat cgaatcgttc ggcaaattgg tcgaggcggt atggcagatg
     9601 tttacctagc aagagatttg attttagatg gagaagaggt tgctgttaaa gttcttcgga
     9661 ccaactatca gacagatcaa attgctatcc agcgttttca acgtgaagcg cgtgccatgg
     9721 cagaacttga ccatcccaat atcgttcgta tttcggacat cggtgaagaa gatggtcaac
     9781 agtatctggc tatggaatat gtcaatggcc tcgatttaaa acgttatata aaagaaaatg
     9841 caccactttc caacgatgtt gcggtgcgaa ttatgggaca gattctcctt gctatgcgca
     9901 tggcgcacac gagaggaatt gtccatcgag atttgaaacc tcagaacgtc cttttgactt
     9961 ccaatggagt cgctaaagta acagactttg gtattgcagt tgcctttgct gaaacgagtt
    10021 tgacacaaac caactccatg ttgggttctg ttcactatct ttcacctgag caggcgcgtg
    10081 gttcgaaagc gaccattcaa agtgatattt atgcgatggg aatcatcctt tttgaaatgt
    10141 tgacgggtcg cattccctat gatggagaca gtgctgtgac gatcgctctt cagcatttcc
    10201 aaaaacctct cccatctgtt agagaagaga atgccaacgt acctcaggcc ttagaaaatg
    10261 ttgtactaaa agcaacggca aaaaaattaa acgagcgcta taagtcggtt gcagaaatgt
    10321 atgcagattt agcttcggct ctatccatgg atcgtcagaa tgaacccagg gttgaactag
    10381 aagggaacaa ggttgataca aaaaccttgc cgaaactctc ccaagccaat gttgaaacga
    10441 aagtaccgca tacaaacagt tctgctcaag tttctgcgac ggataagggg tctggtaaga
    10501 aagaagtggc gaagtcggga aataagcctg tttcaaaacc aaggccaggc atacgtacac
    10561 gctacaaagt cttgatagga gctattttgt taacagtcat agcggctgga ctcatgtttt
    10621 tcaacgctcc acgaacagtt acagtccctg atgtatctgg tcaaaccgtt gaaaaagcta
    10681 cagaaatgat tgaagtggca ggattggaag ttggaaatat cacagaagaa gcgacagcga
    10741 ctgtagatga aggcttggta atacggacct ctccggctgc aaagacgacc agacgtcaag
    10801 ggagtaagat tgatattgta gtagcaacgg ctgcgctggc atctattcca gatgtggtgg
    10861 ataaggagtc tgatactgcg cggcaggaat tggaggccct tggtttccaa gtgacaataa
    10921 aagaagaata tagcgaaaaa gttgcccaag gactcgttat caaaacagat ccaggagcaa
    10981 atagttctgc tgagaagggt gcaaaaatta ccctttacgt ttccaaagga gtggcgccac
    11041 aagttgttcc aaacgttgtt ggaaaaagtc aggagaatgc tactcaaatt cttcagacag
    11101 caggcttcag cattggaacc attactcagg aatatagctc atctgttact gcaggtcagg
    11161 ttatcagcac agatcccgtt gccaatacag agttggcaaa aggttctatc atcaatctag
    11221 ttatttcaaa aggaaaagaa ttgattatgc ctgatttaac atctggcaat tacacctatt
    11281 cacaggcgcg tagtcaatta caagccttgg gtgtcaatgc ggaaagtatt gaaaaacaag
    11341 aagatagaag ctattattca acgacaagtg acatcgtcat tggacaatat cctgctgctg
    11401 gagcaacaat tgatggaaca gttaccttgt atgtctctgt tgcatcaacc agaaccagta
    11461 gcgattcttc agcaggaagt tccacaagta caagtacttc aacaggtagc ggacaataaa
    11521 gatgaataag gttggggaga tacccaatgt cattaagtta tctaacctat cacattttgt
    11581 gatgggttag tttttttatg ttacactaaa aataaaaggg gaaaagtcat gctagatcaa
    11641 attaaagctc atttatactc ttcgaaaatc aaaattatcc gttgtcaact tgccttgatg
    11701 aactccagtt ctatcttcga cttcgtttcc taggctactt ttgattttca ttgagtatta
    11761 cttgatagta tagacaatat ttagttttat agccctttag tttctctttg attacttcat
    11821 caacttgctt tgaactaaaa gtaaactaaa actatatatc agtcagtcct atatttccta
    11881 ctttggatgg agttctttta cttatttttt gttacaatag tatagaaagg atggagaaga
    11941 tgcggaaagt tcaatttttt ctactaatag aaagtatgat ttttacgatg gcagcctttg
    12001 acgtggtagc caatgaggcc agtcggaccc tgctactttt ctctgcctta ttactcgtca
    12061 tttggtactt cctaggaaga aaagtcaaca gtgttttact ggtcagctcc ttatctctat
    12121 tattttcggt ctttgtttta aatcccttct tcattattgg gattatgcta ctagtcgtgt
    12181 acatgctgat taatttcttt tcacgttatg aaaagagaaa ccaatatacc cacattgttt
    12241 ttagtgatta ttctctacag gttcaaaaag agaaaaatag atggtttggc aaccatgacc
    12301 actcacaaga taggtttggt tttgaagata ttaacattgt acgcttgttt ggcaatgatg
    12361 tagttgattt ggataagact gtattagttg gcagagataa tattgttgta attcgtaaaa
    12421 cgtttggacg gacaaaaatt attgttccaa ttgatgttga ggtcgcactt tcagcaacta
    12481 gtatctatgg acgagtaagt ttcctgggtg aatcatactg ggatttgcgg aatgaaagta
    12541 tagcggtcaa tagttccgat tatcaagaat cacacaaacg tgtaaaagtc gttactaata
    12601 atatactggg agatgtggag gtggttcgcg tatgagaaaa cgtacctacc tactcctcgc
    12661 ctggtttgcc tgtcttattg tttttgtggt gattgcttcc accctcccac ttttaaatta
    12721 ttcattgtta gatgttacgc tctggatttc aacggagcag ctcgtgttta ctctcattct
    12781 actagttatt gttttgaccc tttttctagc tgtaatggta caaactgtca gcctagcctc
    12841 cactcaggtt atgcggacga agttacaagc tatcttgaag aataaaagta ttcgaacaga
    12901 taccgaaagt gatcagctct tgctccagtt gtctgataag gtcagaaacc tcacgcgtca
    12961 ggtgcagctg gttgacaatc aagatttagt caagaaagaa gagattgttg agggagaacg
    13021 gaaacggatt gccagggatt tgcatgatac ggttagtcaa gagttatttg caacgagcat
    13081 gattttgtca ggattatcgt ccaatctatc cactcttccg caggaaactt tgcaggaaca
    13141 gctgatgctc gtgaaagaca tgattgagtc tgcccaaaga gacctacgca tcctattatt
    13201 acatctacgc ccaagtgaac ttgaaagtaa aaccttggtt gaaggatttg agctgatttt
    13261 gcgtgaagtt agcgacaaaa gtaatattat cgttcatttc caacatgaag tagaggagtt
    13321 accgaaacta attgaagaac atctcttccg cattgcgcaa gaaattatca gtaatacctt
    13381 gcgacatgcc aaagccaagc atttggatgt ttacctcttg cagaaggaaa cagaacttca
    13441 gttgaaaatg acagacgatg gtatcggctt ccaacaaggg gcagatgatg aattaagtta
    13501 cggtcttcaa aacatacgtg agcgggttga agacatggct ggaacgataa aaattcgcac
    13561 tgctccaaat aagggagtgg cgattgatat tcgtatccca ctattgaaag gaaaagaaga
    13621 tgaatacgat tcgagtgatg ctggttgacg accatgagat ggttcgtcta ggattgaaaa
    13681 gttacttaaa tttacagcca gatgttgaag tagttgcaga agctagtgat ggcgaggaag
    13741 gtttggctaa agcattagag gttaaacccg atgttgtagt gatggacttg gttatgccta
    13801 agatgactgg cgtagaagca accctggcct tgctgaagga atggccgcaa gcgcagattg
    13861 tcatcctgac ctcctaccta gataatgaga aaatctatcc tgtgctggaa gcaggagctc
    13921 gtggctatat gttgaaaacg tcaagtgctg acgaaatctt agccgctatc cgcaaggtag
    13981 ctcttggaga atatgctatt gagacagaag tagagaaaaa agtagagcac cataaacgcc
    14041 acccagattt acatgatgac ttgacagctc gtgaacgcga aatcttgacg ctgttagcca
    14101 agggatatga taatcaacga attgcagatg agtctttcat ttccctaaaa actgttaaga
    14161 cccatgtttc caatatctta tccaaactag ctgttagcga tcgtactcaa gcagttgttt
    14221 atgctttcca gcatggtttg gtggcgcaag aggaccaata gtggtataat gaaaattagt
    14281 taattttcaa ccaaaggata gtggaattat atggatgcaa aactcagata taaggcaaaa
    14341 aaaataaaaa tagtcttttt tgacattgac gacacgctac gtgtaaagaa tacaggctac
    14401 attcctgaat ccattcagca ggttttcaac tcactgaaag aaaagggaat tttgacagga
    14461 attgcgtcag gacgaacgcc ttatggtttg gtgccagaaa tcaaggcctt aaagccagac
    14521 tttttcgcta tgattaacgg ctcctacgta gaagatgcca aaggtcaggt agtgtaccat
    14581 cagccaatgc cccaaaatat agtagaatct gtattaaatt gggctaaaga aattggaatc
    14641 gaatatggta tgcttggtag tcaaaaaggg acactttctg cacgaactga ccgcattagt
    14701 caagtaattg acttgattta tgaaggtttg gaaataaacc caacttttta taaagaaaat
    14761 gacatctatc aattgttaac tttcgagaaa gatggtcacg aagtagaact tccagaagag
    14821 ttgcaagcgg aattgcgtag tgttcgttgg gatgctattt catcggacat tgtgttaaaa
    14881 ggctcttcca aagcaactgg tgttgctaag gttgttgaaa aactaggcct gaaacctgaa
    14941 aatgtcctag tgttcggaga tggactcaac gatattgagt tattcgatta tgcaggaatt
    15001 agtatcgcta tggggcattc gcatccagaa ctgcaaaagc atgcagatta tatcacaaaa
    15061 aaagtagaag aagatggcat ttttgatgcc ttggagaaat taggtatggt agaaaaagaa
    15121 aaatatttcc cgcaattaga tttggaaaat gttacaggac cagttgcgca tatcaagact
    15181 aaccatggta agttaacagt aaaactcttt cctgaaattg cacccaaaac tgtagcaaat
    15241 ttcgtagctc tttctaaaga tggctattat gacgggatta tctttcatcg tattatcaaa
    15301 gactttatga ttcaaggtgg cgatccaacc ggaacaggta tgggtggtga atctatttat
    15361 ggtactgcgt ttgaagatga attttcaatg gaagctttta atctcagagg agccttgtca
    15421 atggccaatg caggaccaaa tacaaatgga agccaattct ttatcgttca aaaccaaaat
    15481 ttcccataca atgcgaaaga attagaacga ggcggttggc ctaaagaagt tgcagaagcc
    15541 tatgtcaaaa atggcgggac accacatctg gatcaacgcc acacagtgtt tggtcatcta
    15601 gttgatgaag aatccttcgt cgtcttggat gctatcgcag cggttgcaac agattcagca
    15661 gatcgtccac atgaagatgt tgtaattgag accatcgaaa tagaggatta agatgaaaat
    15721 cggagataaa ctcaagggga cggtaacggg tattcaaccg tatggagcct ttgtacaatt
    15781 agagaatggg acgacgggcc taatccatat ctcggaaatc aaaacaggtt ttgttgataa
    15841 tatttatgat catttaaaat tggggcaaga ggttttggta caggtagttg actttgatga
    15901 atatactgaa aaggctagtt tatcaatccg gaccttggaa gaagaacaac aaaaaattcc
    15961 gcgtcatcat cgttttacaa atgaacggaa taaaattggt ttttcccctt tagcaaaaca
    16021 acttccgaaa tggattgagg aagctaaagg ctatctcaaa gaacacaaag aaaagaaggt
    16081 ataggatgca ttcctaacct tctttctttt ttaatcattg aactcataaa gagctgttga
    16141 caagtaacgt tctccgttat caggtaagat tgccaacact ttttttccag ttcccaactc
    16201 cttagcgact tcgatagcag cgtgaattgc tgcacctgaa gaaataccaa ctaagaatcc
    16261 ttcttttcca ccgatagcac gacctgtagc taaggcatca tcggatttga cacgaataat
    16321 gccatcatag gcgctagtgt ccaaagtatc tggaataaaa cctgccgaaa taccttggat
    16381 cttgtgcgga ccaggtgctt caccagataa gacagctgat tcatcagctt cgacagcata
    16441 gatagcaata tctggatttg ctgtcttcaa aacatgcgaa acacctgaaa cggtaccacc
    16501 agtaccaacg ccagatacaa aggcatctaa accagttgtt ccaaagtctt ccaaaatttc
    16561 ctgtcctgtt gtatcttcgt gaacttttgg attggatgga ttagcaaact ggaaaggaac
    16621 ccagccattt ttttcttcag caatctcttt tgctttggcg atagccccct tcatcccttc
    16681 gctaccagga gtcaagacaa gctcagctcc ataagcttgg ataatcttac gacgctcaac
    16741 actcatagtt tcaggcatga cgataatcac cttataacct tttgctgctc caacccaagc
    16801 aagaccgata ccagtgttac cacttgttgg ctcaacaata gtatctccag gtttgattgt
    16861 accagccttc tcagcatctt caatcatagc caaggcgatc cggtctttta cagaagatcc
    16921 tgggttaaag gcctctaatt tcacatagac ttccgctgcc ccctctggaa cgatattatt
    16981 gagtttaata acaggtgttt ttccaactaa ttgagtaatg ttttgataaa tagccatatg
    17041 gcacct
//
LOCUS       DBNFEG010000028        16806 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.028, whole genome shotgun sequence.
ACCESSION   DBNFEG010000028 DBNFEG010000000
VERSION     DBNFEG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16806
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.028"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(316..786)
                     /gene="rpsG"
                     /locus_tag="AC1PNG_000635"
     CDS             complement(316..786)
                     /gene="rpsG"
                     /locus_tag="AC1PNG_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIP6701156.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKLVTRLINRVMLDGKRGTAASIVY
                     GAFDQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARNRGEHTMIDRLAKEIMDAANNTGAAVKKREDTHKMAEANRAFAHFRW"
     gene            complement(803..1216)
                     /gene="rpsL"
                     /locus_tag="AC1PNG_000636"
     CDS             complement(803..1216)
                     /gene="rpsL"
                     /locus_tag="AC1PNG_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIP6701157.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSRKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKG"
     gene            complement(1448..3070)
                     /gene="groL"
                     /locus_tag="AC1PNG_000637"
     CDS             complement(1448..3070)
                     /gene="groL"
                     /locus_tag="AC1PNG_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014633644.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIP6701158.1"
                     /translation="MAKEIKFAADARESMVRGVDILADTVKVTLGPKGRNVVLEKAYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPIGIRRGIEAAVATAVESLKAQASPVSNKAEIAQVAAVSSRSEKVGEYI
                     SEAMERVGTDGVITIEESRGMETELDVVEGMQFDRGYLSQYMVTDNEKMVAELENPFI
                     LITDKKISHIQDILPLLESILQTSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLDLKDATIEALGQAAKVVVDKDGTTIVEGV
                     GNAEAIANRVAVIKSQIEGTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALVNVIDSVAKLELKGDDETGRNIVLRALEEPVRQ
                     IAYNAGYEGSVIIDKLKNSELGTGFNAATGEWVNMMDAGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPAAPAMPQGMDGMGMGY"
     gene            complement(3082..3363)
                     /gene="groES"
                     /locus_tag="AC1PNG_000638"
     CDS             complement(3082..3363)
                     /gene="groES"
                     /locus_tag="AC1PNG_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIP6701159.1"
                     /translation="MLKPLGDRIVVKIEEKEQTVGGFVLAGASQEKTKEASVLAIGQG
                     IRTLNGDLVAPAVAVGDTVLIDAHAGLEVKDGEQTVHIIREADILAIVE"
     gene            3561..3806
                     /locus_tag="AC1PNG_000639"
     CDS             3561..3806
                     /locus_tag="AC1PNG_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701160.1"
                     /translation="MKFFRELKTDYLESRFSVHESFAEWFLKRKLGFLGKIMFAYLLW
                     LVWLLLFSHPHYIIFFFYGVLLLSLIIMLIEWWKYRK"
     gene            complement(3920..4315)
                     /locus_tag="AC1PNG_000640"
     CDS             complement(3920..4315)
                     /locus_tag="AC1PNG_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIP6701161.1"
                     /translation="MYNKVILIGRLTAQPELTQTPNGKNLTRVTVAVNRRFKTENGER
                     EADFLNVIFWGKLAETLVSYGNKGSLISIDGELRTRKYEKDGSNHYVTEILGQSFQLL
                     ESRAQRAMRENNAGDDLADLVLEEEELPF"
     gene            4370..5149
                     /locus_tag="AC1PNG_000641"
     CDS             4370..5149
                     /locus_tag="AC1PNG_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HIP6701162.1"
                     /translation="MKEFLMEKIHTSQPYTNAELAWLLENISHPDPAIRDELVYASFC
                     HIFLEGLITREQAQSLLQFSQETNHLSLESSTLKRSFTCLLYCLLLSVDNERDSVYFA
                     FLSTNDRELLFQQALDYLAIENDWSGYDEKLGWIHTAAHGADFLLATSCHDQFPAEKS
                     KEVWQTIVTCLTRQSKVFSAGEEIRLAQIPVYLLLNEKVTSKELTEWISKLDFPNQEP
                     VDYFRWLNLQHFLSSLYFQLRSHQASTEEIEQAIEGKIEPA"
     gene            complement(5183..5806)
                     /gene="ytpR"
                     /locus_tag="AC1PNG_000642"
     CDS             complement(5183..5806)
                     /gene="ytpR"
                     /locus_tag="AC1PNG_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HIP6701163.1"
                     /translation="MIFTYNKEHVGDVLMVIVAEDKGQPVQFERKGQVARVFLEETGK
                     TVAWNIFEASSLVELAGNGQVFMTDEQVATLNAELAKEGFTESLVNDNSPKFVVGQIV
                     ELVPHPDSYHLNICQVNVGDKTVQIVAGAPNAAQGLKTIVALPGAMMPSGSLIFPGKL
                     RGEDSFGMMCSPRELALPNAPQVRGIIELDDSAVVGEALDPAKHWKG"
     gene            complement(5825..6781)
                     /locus_tag="AC1PNG_000643"
     CDS             complement(5825..6781)
                     /locus_tag="AC1PNG_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HIP6701164.1"
                     /translation="MKKSLGKILLAPVVLGLSLSLAPLVQAEIQTDVINEKWGKPTLV
                     YGGGLSDAQATEVNNLFGISDVNNVSRQVVVGTDIDQYLGTSEADTASLYSSVLVQKQ
                     DAGKGVVVDIKTPQNITLITETQYANAAITAGATDVLIDVAAPIQVTGESALTGVYKA
                     LAANGDTVDTARTEVAQQELTTVNEVATAHAGDEQFDSSALDKAVAEIKTALADYKKS
                     NGQVASEADINTIINDVLTQNGLENVITADEISKLVTFAKAYQETSAIDSAEVAAQLN
                     QFKQQAEQQISEAYKNLQDSGILEKIGAFFENLWKGLTGLFA"
     gene            complement(6826..7146)
                     /locus_tag="AC1PNG_000644"
     CDS             complement(6826..7146)
                     /locus_tag="AC1PNG_000644"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIP6701165.1"
                     /translation="MIFPTNLEEVAALIEDGKPTVFLFVTTWCGDCHYIKPHLSAIEE
                     AFPDFRFVQLDRDDFMPLAQEWAILGIPSLVVLENGQEIGRFVDKNRKTKEEIMNFLS
                     GLGR"
     gene            complement(7143..7427)
                     /locus_tag="AC1PNG_000645"
     CDS             complement(7143..7427)
                     /locus_tag="AC1PNG_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HIP6701166.1"
                     /translation="MKKKKTALLAGLLGAGVLTASAQFYRKIQEDRKKAQALQEVRDF
                     FADLGEIATVYVDETASTQTTLIGGVVMEAGQVFLFENTKGSIQYREEER"
     gene            7574..8635
                     /gene="pepA"
                     /locus_tag="AC1PNG_000646"
     CDS             7574..8635
                     /gene="pepA"
                     /locus_tag="AC1PNG_000646"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HIP6701167.1"
                     /translation="MTLFEKIKEVTELQSLPGFEGQVRNHIRQKITPHVDRIETDGLG
                     GIFGIKDTAVENAPRILVAAHMDEVGFMISQIKPDGTFRVVELGGWNPLVVSSQAFTL
                     QLQDGRTIPAISGSVPPHLSRGANASGMPAVADIIFDAGFANYDEAWAFGVRPGDVLI
                     PKNETILTANGKNVISKAWDNRFGVLMVTELLESLSGQALPNQLIAGANVQEEVGLRG
                     AHASTTKFNPDIFLAVDCSPAGDIYGDQGKIGDGTLLRFYDPGHIMLKNMKDFLLTTA
                     EEAGVKFQYYCGKGGTDAGAAHLKNHGVPSTTIGVCARYIHSHQTLYSMDDFLEAQAF
                     LQAIVKKLDRSTVDLIKNY"
     gene            complement(8680..9936)
                     /locus_tag="AC1PNG_000647"
     CDS             complement(8680..9936)
                     /locus_tag="AC1PNG_000647"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIP6701168.1"
                     /translation="MNYLEMRQWLSSRPASDLEHGVARVNWILERLDNPQLQVSTIHF
                     VGTNGKGSTLNALQSILQASGYIVGCFTSPSIIDFREQIVYQQEMISEEDFARIVTDL
                     QPLIEDLDQTAGLDAISEFEIVVVAMFVYFAHYQRPDILLVEAGMGGLQDATNVLAPL
                     AVVCPSIGLDHQAFLGETHAAIARHKVAVLRERVPLIYATDLPEVETVFEEHACQLQS
                     PTYAVGRDILLENSRAGFAVSSPLGRVEDLRLQMQGRHQEVNAALAVTTTQLLSLDFP
                     KITKETIRQGLSQAIWPGRLELIRPNLIIDGAHNNESIAVLTQLLEEKYADRDIEILF
                     AAINTKPVDQMLSQLSQFGPVSVTTFDDFRAVQLEDYPSGYERVQTYQEWLEQADLDN
                     PQKLYLITGSLYFITYVRKYILEELV"
     gene            complement(9990..10541)
                     /locus_tag="AC1PNG_000648"
     CDS             complement(9990..10541)
                     /locus_tag="AC1PNG_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HIP6701169.1"
                     /translation="MEKKIPKLTVQLLAAIAMTLALVMIVENYFSIRISDTLQVQFTF
                     VPNTILGAIAGPVWAAVFAAISDPVFVLFSGQTVLFTWILIEAVSAFIYGWFFYRKPL
                     DTKNKADWLYVAGVVVLIQVVISFIMTPIALHFHFGTPWIVLYSSRLIKAVFEIPLRI
                     VVTMLVLPSLQKIPELAKLMGIK"
     regulatory      complement(10586..10730)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            complement(10856..12043)
                     /locus_tag="AC1PNG_000649"
     CDS             complement(10856..12043)
                     /locus_tag="AC1PNG_000649"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HIP6701170.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVLAAGIVERIGLNDSISTV
                     KYDGKKESEVLDIPNHTVAVKILLEDLLKHNIIAAYEEITGIGHRVVAGGEIFKESAV
                     IGPKEVEQIEELSALAPLHNPAHVAAMRAFEEVLPEVLNVAVFDTAFHTTMPKHAYLY
                     PIPQKYYTDHKVRKYGAHGTSHYYVAHEAAKVLGRPIEELKLITAHIGNGVSITANYH
                     GQSVDTSMGFTPLAGPMMGTRSGDIDPAIIPYLIANVDELKDAADVVNMLNKQSGLLG
                     VSGYSSDMRDIESGLEAKNPDAVLAYNIFIDRIKKFIGQYFAVLNGADALVFTAGMGE
                     NAPLMRNDVVEGLSWFGVEIDPAKNVRGANGDISTPESKVRVLVIPTDEELVIAREVE
                     RLK"
     gene            complement(12093..13046)
                     /locus_tag="AC1PNG_000650"
     CDS             complement(12093..13046)
                     /locus_tag="AC1PNG_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIP6701171.1"
                     /translation="MNFEKIEQAYDLLLENVQTIQNQLGTNIYDAMIEQNAAYVANQH
                     ETDLVVNNNKTLKQLDLTKEEWRRAYQFLLIKANQTEPMQYNHQFTPDSIGFILSFLV
                     DQLVPTQKVTVLEIGSGTGNLAQTILNASQKELDYLGIEVDDLLIDLSASIADVMQAD
                     ISFAQGDAVRPQILKESQVILGDLPIGYYPDDQIASRYQVASPNEHTYAHHLLMEQSL
                     KYLEKNGFAILLAPNDLLTSPQSDLLKGWLQEQANIVAMIALPPNLFGKAAMAKSIFV
                     LQKKAARSLTPFVYPLQSLQEPEAIQKFMLNFKNWKQENAI"
     gene            complement(13103..13513)
                     /gene="comGG"
                     /locus_tag="AC1PNG_000651"
     CDS             complement(13103..13513)
                     /gene="comGG"
                     /locus_tag="AC1PNG_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HIP6701172.1"
                     /translation="MLLKKKVKAGILLYALLMLAVFSLLLQFYLHHQEAESRLVQVAR
                     QETTAYIMAQMVLDKVEQDLQVEKIVTGAVVNEKEANLTTGSKQKENRGRVSFQEGQA
                     RYQLKGDQLLVTVELTSDGLYTYQFPLGLSSEGG"
     gene            complement(13491..13925)
                     /gene="comGF"
                     /locus_tag="AC1PNG_000652"
     CDS             complement(13491..13925)
                     /gene="comGF"
                     /locus_tag="AC1PNG_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HIP6701173.1"
                     /translation="MLKSKIPAFTLLECLVALVILSGSLLVFEGLSKLLIQEAHYQRQ
                     SVQKEWLVFSSQLRSEWDRSELVKVENGKVYVDKGGQSLAFGKSRSDDFRKTNDRGQG
                     YQPMLYQVDSAAISQENQLVRIDFSFKNGEERTFIYAFKEKS"
     gene            complement(13912..14205)
                     /gene="comGE"
                     /locus_tag="AC1PNG_000653"
     CDS             complement(13912..14205)
                     /gene="comGE"
                     /locus_tag="AC1PNG_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002282213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGE"
                     /protein_id="HIP6701174.1"
                     /translation="MVAIRKQKNKAYILLESLVALATLVTICSLILSAVDAGRRRQAW
                     ELEQQEVLNLAQMAVQTEQDNLALNGVTVQVKRTADRVAVFHEGKEVLSVVKK"
     gene            complement(14177..14584)
                     /gene="comGD"
                     /locus_tag="AC1PNG_000654"
     CDS             complement(14177..14584)
                     /gene="comGD"
                     /locus_tag="AC1PNG_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HIP6701175.1"
                     /translation="MLRRKNKAFTLFESLLTLFVVSFLAVSLSGTVQTVFRSVQEEIF
                     LWEFEAIYKDSQKLAASSHQSVSLTIGEQEVANGRQAVQVPRNVEVLEGKTITFEEDG
                     GNSSLTKIRFRLSRKTVTYQLYIGSGRYKKTEE"
     gene            complement(14565..14846)
                     /gene="comGC"
                     /locus_tag="AC1PNG_000655"
     CDS             complement(14565..14846)
                     /gene="comGC"
                     /locus_tag="AC1PNG_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HIP6701176.1"
                     /translation="MKKLVEMKVKGFTLVEMLVVLGIISLLLLLFVPNLSQQKDAIQK
                     KGDAAVVKVVESQMELYELEHDEEATVADLQAKGYITEKQAKQYATAKK"
     gene            complement(14848..15885)
                     /gene="comGB"
                     /locus_tag="AC1PNG_000656"
     CDS             complement(14848..15885)
                     /gene="comGB"
                     /locus_tag="AC1PNG_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HIP6701177.1"
                     /translation="MRKLIAFLQQDISVLGRQKQKKLPLARQRKVIELFNNLFASGFH
                     LGEIVDFLKRSQLLADLYTQVLSDGLLAGKPFSSLLADLRFSDAVVTQVALAEVHGNT
                     SLSLSHIQSYLENVSKVRKKLIEVATYPVILLGFLLLIMLGLKNYLLPQLEEGNAATV
                     LINHLPTIFLSLSGLSLVAVLAGMVWFRKTNKIKVFSCLAALPFFGKLIQTYLTAYYA
                     REWGSLIGQGLDLPQIVGLMQEQQSQLFREIGQDLEQSLSNGQSFHEHIKTYAFFKRE
                     LSLIVEYGQVKSKLGSELTVYAAECWEDFFSRVNRAMQLIQPLVFLFVALMVVLIYAA
                     MLLPIYQNMEL"
     gene            complement(15797..16747)
                     /gene="comGA"
                     /locus_tag="AC1PNG_000657"
     CDS             complement(15797..16747)
                     /gene="comGA"
                     /locus_tag="AC1PNG_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HIP6701178.1"
                     /translation="MIQEKARKLIEKAVADRVSDIYLVPRGESYQVYHRIMDEREFVQ
                     DLAEEEVTAIISHFKFLAGLNVGEKRRSQQGSCDYDYGSGEISLRLSTVGDYRGKESL
                     VIRLLYDNDKELKFWFEAAERLAEEIKGRGLYLFSGPVGSGKTTLMYHLARLKFPDKQ
                     ILTIEDPVEIKQEDMLQLQLNEAIGATYDNLIKLSLRHRPDLLIIGEIRDAETARAVI
                     RASLTGATVFSTVHARSISGVYARMLELGVSPEELNNALQGIAYQRLIRGGGVVDFAK
                     GNYQNHSADQWNAQIDRLFAAGHISLRQAETEKIALGSPA"
BASE COUNT         4630 a         4090 c         3310 g         4776 t
ORIGIN      
        1 tccgatttgt gatggttgaa cgataaattg ctaccataat ctcaattgat ttatgtagaa
       61 ctgacttaac gtcctcacat cctaattgaa ctcgggctac aactcactga caaaaagatg
      121 aacctcatcg attgcacagc aatctgcttc gcttccctat tttgtctagg agttgcttga
      181 cgccctcgta tcttaatctt agttgaattt tagctacaaa tgtctgccaa aaagataaaa
      241 tctcctagaa actgaagttt ctgcgtcaat tttcctattt tggctagcat ttgtttaacg
      301 ctaaaatatc ctatcttacc agcggaagtg tgcgaatgcg cggtttgctt ctgccatttt
      361 gtgagtatct tcacgtttct taacagctgc acctgtgttg tttgctgcat ccatgatttc
      421 tttcgcaagg cgatcgatca tagtgtgctc accacggtta cgagcgattg ttaccaacca
      481 acgaagacca agtgttgtac gacgctctgg acgaacttca actggaactt ggtagttaga
      541 accaccgaca cgacgtgcac gtacttcaag tacaggcatg atgttttcca ttgctgtttc
      601 aaatacttca agtgcatcgt taccagttgc ttctttgatt tgatcaaagg caccgtaaac
      661 gattgatgca gcagtaccac gtttaccgtc caacataaca cggttaatca aacgagttac
      721 caattttgag ttatacaatg gatctggcaa tacttcgcgc ttaggcgctt gatttttacg
      781 actcatttat atttcccctt tcttagcctt ttggacgttt agtaccgtat ttagaacggc
      841 cttgcttacg atcgttaaca cctgctgtat caagtgcacc acgaacgata tggtaacgta
      901 cccctggaag gtcttttaca cgtccaccac gaagaagaac aacactgtgc tcttgcaagt
      961 tgtgaccgat acctgggatg taagcagtaa cctcgataag gttgctcaaa cgtacacgag
     1021 caaatttacg aagggctgag ttaggttttt taggtgtcat agttccgaca cgagttgcaa
     1081 caccgcgttt ttgtggtgat gaaacgtttg tttgaacttt tttacggctg ttgtaaccaa
     1141 cgttcaaagc tggtgattta gatttttcta ctttagactt acgtggttta cgtaccaact
     1201 ggttaattgt aggcatctac attctcctgt atatattttt tatttttggt gatagggcat
     1261 tggtgacaac ccctatctgt gtgtactttt gcaacatttg tcagcacgtc cctgtacact
     1321 cttgagagac caaaagtaaa aagtaccgtc taatatagta ccacatccca aagccattgt
     1381 caaatagata tgtttgaaat tttcaagaaa aaaacacagc caatcagctg tgtcattttg
     1441 ctattcctta gtaacccata cccattccgt ccataccttg tggcatagct ggagcggctg
     1501 gttctggctt gttggcaacg actgcttctg tcgtcaaaat caagctggct acagaagctg
     1561 cgttttgaag ggccgaacga gtcaccttaa cagggtcaat aatacctgca tccatcatat
     1621 tcacccattc acctgtcgca gcattaaagc ctgttccaag ctcagaattc ttcaatttat
     1681 caatgataac tgatccctca taaccggcgt tataggcaat ctgacgtact ggttcttcca
     1741 aggcacgaag gacaatattg cgtcctgttt catcgtcgcc tttcaactcc aatttcgcta
     1801 cgctatcgat aacattgaca agggctgtac caccaccggc aacgatgcct tcttcaactg
     1861 cggcacgtgt tgcgttgagg gcatcttcga tacggagttt catttctttc aactcagtct
     1921 cagtagcggc accgactttg ataacagcaa caccgccaga caatttagcc aaacgctctt
     1981 gtaatttttc acggtcaaat tctgaagttg ttccctcaat ttgtgacttg ataacagcca
     2041 cacggttggc aatggcttca gcattgccaa caccttcaac aatagttgta ccatccttgt
     2101 caaccacaac cttggcagct tgaccaagtg cttcaatagt cgcatctttc aagtccaaac
     2161 caagatcttc tgtaatcact gtcccacctg tcaagatcgc gatgtcttcc aacatagctt
     2221 tacgacggtc gccaaagcct ggcgccttaa cagcaacaac gttgaacgta ccacgaatct
     2281 tattgagaac aagggtagga agtgcttcac catccacatc gtcagcaata atcaaaagcg
     2341 gacgactagt ctgaaggatg ctttccaaaa gtggcaaaat atcttggatg tgagaaattt
     2401 tcttatcagt aatcaagata aatggatttt caagctccgc aaccatcttc tcattatctg
     2461 ttaccatgta ttgtgacaga taaccacggt cgaattgcat gccttctacc acatccaact
     2521 cagtttccat accgcgagat tcttcgatag tgattacgcc gtctgttccc acgcgctcca
     2581 tagcttctga aatgtactca ccaacttttt cagaacgtga agataccgct gcaacttgag
     2641 caatttcagc tttattggat acaggacttg cttgtgcttt caaggattca accgcagtcg
     2701 caactgctgc ttcaatcccg cgacggatac caattgggtt ggcacctgca gttacgtttt
     2761 tcaatccttc acgaacaata gcctgagtca acacagtggc cgttgttgta ccgtcaccag
     2821 cgatgtcatt ggtttttgat gccacttcag ataccaactt ggcacccata ttttcaaagt
     2881 ggtcttccaa ttcgatttct ttggcaatgg ttacaccatc atttgtaatg agtggtgagc
     2941 cataagcttt ttccaagaca acattgcgac cttttggacc caaggttact ttgactgtat
     3001 cagccaaaat atcaacacca cggaccatgc tttcacgtgc atctgctgca aatttgattt
     3061 cttttgccat tttcctacct cttattctac aattgccaaa atgtctgctt cacggatgat
     3121 gtgaacggtc tgttctccgt ctttgacttc caatcctgcg tgagcatcaa tcaaaacagt
     3181 atctccaact gctaccgctg gggcaaccaa gtcaccattc aaggtacgaa tcccttgacc
     3241 gatcgctagg acacttgctt cttttgtctt ttcttgactt gcacctgcaa ggacaaagcc
     3301 accaactgtt tgttcttttt cttcaatttt tacgacgata cgatcgccca atggtttcaa
     3361 catcttatta cctcctggtc tttttagcac tctacactcg cgagtgctaa ctcacatttc
     3421 ctattgtatc acttggtcaa aaatagtcaa gaaaattctt ttttaattta cctaaaatcc
     3481 gtctagggac tgaggaaacg caacctgtta attatggcaa aactatgcta tactattatt
     3541 aaaaagttaa ggggggattt atgaaatttt ttagagagct aaaaacagat tacctagaaa
     3601 gccgtttttc cgttcacgaa agttttgcag aatggttttt aaaacgtaaa ttaggctttt
     3661 tggggaaaat aatgttcgcc tatctactct ggctagtctg gttgcttctc ttttcccacc
     3721 ctcattacat catctttttc ttctacggag ttctcctact ttctctaatt atcatgctga
     3781 tagaatggtg gaaatataga aaataagtag caaaaaacgc caactttcgt cagcgttttt
     3841 aaggagatat gaaaaatatt taggattgac tatagtatag cctatttttc aatacgctcc
     3901 atcggtcttg ggactgatat taaaacggta attcctcctc ttctaagacc aaatcagcta
     3961 gatcatcgcc agcattattt tcacgcatag cacgttgggc ccgactttcg agcagctgga
     4021 aggattgtcc taagatttct gtcacatagt ggttgctgcc gtctttttcg tattttcgcg
     4081 ttcgcaattc accatcaatg gaaattagac tacctttatt accataggaa acaagcgttt
     4141 ccgccagttt gccccagaaa atgacattaa gaaaatctgc ttcacgttcg ccattttccg
     4201 tcttaaaacg gcggttgact gcgacagtaa cacgggtcag atttttgccg ttaggggttt
     4261 gagtgagttc aggttgggcc gtcaagcgac cgattaaaat aactttatta tacattttat
     4321 atcctcctac ttatttattc gtagaaggat tagaaaagga gcgaaaatta tgaaagaatt
     4381 tttgatggaa aagatacaca cctcccaacc atatactaat gccgaactag cttggttgct
     4441 ggaaaacatc agtcatcctg atcctgctat acgcgacgaa ctggtttatg ctagtttttg
     4501 ccacatcttt ttggagggct taatcacaag ggagcaagca cagtcgctac tacaattctc
     4561 tcaagaaacc aatcaccttt ccctagaaag ttccacacta aaacgaagtt ttacttgctt
     4621 gctctactgt cttttgctat ctgttgataa tgagcgagat tctgtgtact ttgcattttt
     4681 aagcactaac gaccgtgagc tactcttcca gcaggccttg gattatttgg cgattgaaaa
     4741 tgactggtct ggctacgatg aaaaactcgg ctggatccac accgctgccc acggagcaga
     4801 ttttcttctg gccacaagct gtcatgatca atttcccgct gaaaaaagca aagaagtctg
     4861 gcaaactatt gtcacttgtc taactagaca aagtaaagtt ttctcagctg gcgaggaaat
     4921 ccgtctggca caaattcctg tatatttact actgaatgaa aaagtaacca gcaaagagtt
     4981 gacagaatgg attagcaagc tggactttcc aaatcaagaa ccagtagact acttccggtg
     5041 gctcaatctc caacattttc tgtctagtct ttacttccaa cttaggtcac atcaagcatc
     5101 gaccgaagaa atcgaacagg ctattgaagg aaaaattgaa ccagcataaa agaattggcc
     5161 aaagcgacca attcttcttt ttctatccct tccagtgttt agctggatca agtgcttctc
     5221 cgactactgc ggagtcgtcc aattcgataa tgccacggac ttgtggtgcg tttggcagag
     5281 ccaattcacg cggactacac atcataccaa agctatcttc accacgcaat ttccctggga
     5341 aaatcaggct accgctcggc atcattgccc ccggaagagc cacaatagtt ttcaatcctt
     5401 gggcagcatt tggtgctcca gctacgattt gaacggtttt gtcaccaaca ttgacctgac
     5461 agatattgag gtggtagctg tctggatgcg gaaccagttc cacaatctga ccgacaacga
     5521 atttcggact attgtcgttg actaagcttt cagtaaaacc ttccttagcc aattcagcat
     5581 tcaaggtcgc aacttgctcg tctgtcatga aaacctgacc atttccagca agctcaacca
     5641 aacttgatgc ttcaaagata ttccaggcta ctgtttttcc tgtttcctct aggaaaacac
     5701 gcgccacttg acccttgcgt tcaaactgaa caggctgacc cttgtcttca gccacaatca
     5761 ccataagaac atcgccgacg tgttccttat tatatgtaaa aatcatttct ttttcatttc
     5821 tcctttatgc aaatagaccg gtcaagcctt tccaaagatt ttcaaagaag gcaccgattt
     5881 tttctaaaat ccctgaatct tgtagattct tataggcttc tgaaatttgt tgctccgcct
     5941 gttgcttgaa ctggttgagt tgggcagcaa cctcagccga atcaatagca gaggtttcct
     6001 gataagcttt ggcaaatgtg actagcttag aaatttcatc tgctgtgatg acattttcta
     6061 aaccgttttg ggtcaaaaca tcattgatga tggtgttgat gtcagcctca gaagccacct
     6121 gaccattgga ctttttgtag tcggcaagag cagtcttgat ttccgccact gccttatcca
     6181 aagcagagct gtcaaattgt tcatcaccag catgggctgt cgctacttca ttaacagttg
     6241 ttaattcttg ttgagctact tcagttcgag cggtatcaac tgtgtcacca ttggctgcaa
     6301 gagccttgta aacacctgtc agagcggact ctcccgtcac ctgaatgggt gccgcaacgt
     6361 caatcaagac atctgtcgca ccagctgtaa tagccgcatt ggcatactgc gtttcggtaa
     6421 tcaaggtgat gttctgtggc gtcttaatat ccaccacaac ccctttgcca gcgtcctgct
     6481 tttgcaccaa gacagatgaa tagagggagg ctgtgtccgc ttcagatgta cctaaatatt
     6541 ggtctatatc cgtaccaaca accacctgac gactgacatt attgacatcc gaaataccaa
     6601 atagattgtt aacctctgtc gcctgcgcat ctgacaaacc tccaccgtag actagtgttg
     6661 gcttgcccca tttttcattg ataacatctg tttgaatctc agcctgcacc aagggagcaa
     6721 gactcaaact caaccccagc accacaggtg ctagaaggat ttttcctagt gatttcttca
     6781 tgttctttcc tttcttttgg gaaatggcag agcttctctg ctaatttatc tacccagtcc
     6841 agataagaag ttcatgattt cttccttggt cttgcgattc ttatcgacaa aacgaccgat
     6901 ttcctgacca ttttccaaaa caactagact tggaatgcct aaaatagccc attcctgcgc
     6961 caaaggcata aagtcatctc ggtctagttg cacaaaacgg aaatctggaa aagcctcttc
     7021 gatagctgac aaatgcggtt tgatatagtg gcagtctcca caccaagtcg tcacaaatag
     7081 aaaaacagtc ggcttgccat cttcaatcaa ggcagctact tcttcaagat ttgttggaaa
     7141 aatcatcttt cttcctccct gtattgaata gaacctttgg tattttcaaa caaaaatacc
     7201 tgtcctgcct ccatcacaac ccctccgata agagtggtct gcgtagaagc cgtttcatcc
     7261 acatagaccg tggcaatctc tcctaaatca gcgaaaaagt cacgcacctc ctgcaaggcc
     7321 tgtgcttttt tacggtcttc ttggatttta cggtaaaatt gagcactagc ggtgagcaca
     7381 cctgccccca ataagcctgc tagcaaggct gtctttttct ttttcatgtt gactatttta
     7441 tcatatttcc caggaatttt cccaaaaatg ccattggact tgtgacagct gagaagatcc
     7501 tgttgacata atagtaggac aagtctagct cttcgtgata aaatagtagt aatcatgtca
     7561 tggaggaaga agaatgactc tttttgaaaa aattaaagaa gtaaccgaat tgcaaagttt
     7621 accaggattt gaaggacaag tccgcaacca tatccgccaa aaaatcacgc cacatgtgga
     7681 ccgcattgaa acagatgggc taggtggaat ttttggtatc aaggatacgg ctgttgaaaa
     7741 tgctcctcgt atcctagtgg ccgcccacat ggatgaagtc ggctttatga ttagccaaat
     7801 caagcctgac ggtactttcc gtgttgttga gctaggtggc tggaatcctc tggttgtttc
     7861 tagccaagcc ttcactcttc agttgcagga cggtcgtacc attccagcca tctcaggctc
     7921 agtaccacct cacctttcac ggggggctaa cgcttctggc atgcctgccg ttgctgatat
     7981 tattttcgat gctggttttg ccaattatga cgaagcttgg gcgttcggcg ttcgtccagg
     8041 ggatgtcctc attcccaaaa atgaaaccat tctaacagcc aacggaaaaa atgttatttc
     8101 taaggcttgg gacaaccgct tcggtgtcct tatggtgacg gaattgctag aaagcctatc
     8161 cggtcaagct ctaccaaatc agttgattgc tggggccaat gtgcaagaag aggttggtct
     8221 tcgtggtgct catgcctcta caaccaagtt caatccagac attttcctcg ctgttgattg
     8281 ctcgcccgca ggtgatattt atggcgacca aggaaaaatt ggtgacggaa ctctgcttcg
     8341 tttctatgac ccaggtcata tcatgttaaa aaatatgaag gatttccttc taactactgc
     8401 tgaagaagct ggtgttaaat tccaatacta ctgtggtaaa ggtggaacag atgccggtgc
     8461 agcccacttg aaaaatcatg gtgttccatc aacaactatc ggtgtctgcg ctcgttacat
     8521 ccactcccat caaactctct acagcatgga tgacttctta gaagctcaag ccttcttgca
     8581 ggctatcgtt aaaaaactag accgctcaac ggttgatttg attaaaaatt attaaaacaa
     8641 aagactttgc tgggttgaat gcccggcaaa gccttttttc tatacaagtt cttctaaaat
     8701 gtacttcctc acataggtaa tgaaatatag cgagcctgta atcaggtaga gtttttgggg
     8761 attgtccaag tccgcctgct ccaaccactc ctgataggtc tgaactcgtt cataacctga
     8821 cggataatct tctaactgta ccgctctgaa atcgtcaaat gtcgtaacgc tgacaggtcc
     8881 aaattggcta agctgggaca acatctggtc cactggcttg gtattgatgg ctgcaaagag
     8941 gatttcaata tccctgtcag catacttttc ttccaagagt tgtgtcagga cggcgatact
     9001 ttcattattg tgggcaccgt caattatgag attaggccta atcaactcta agcggcccgg
     9061 ccagatggct tgggacaagc cctggcggat ggtttccttg gtaatctttg gaaaatcaag
     9121 gctgagaagc tgagttgttg tcactgccaa ggctgcattg acctcctggt gacgcccctg
     9181 catctgtagc ctcaaatcct caacacggcc gagaggactt gaaactgcaa agcctgctct
     9241 gctattttcc aaaagaatat cccgtcccac cgcataggtc ggactctgaa gctgacaggc
     9301 atgctcctca aatactgtct ccacttctgg caaatcggtc gcatagatga gcggaaccct
     9361 ctcgcgcaag acagcaacct tgtgacgggc tatagcagcg tgggtctctc ccaaaaaagc
     9421 ctgatggtcc aagccgatgg acgggcaaac tactgccaag ggagcaagga cattggtcgc
     9481 atcctgcaaa ccgcccatgc ctgcctccac caagagaata tcgggacgct ggtagtgggc
     9541 aaagtagaca aacatagcca ctactacaat ctcaaactcc gagatggcat ccagtccagc
     9601 cgtctggtcc aagtcctcaa tcaagggttg caagtctgtc acaatcctcg caaaatcttc
     9661 ctccgaaatc atctcttgct ggtagacaat ctgctctcga aaatcaatga tagacggtga
     9721 tgtaaagcag ccgacgatgt agccagaagc ctgtaggatg gactgtaagg cgttgagggt
     9781 cgagcccttg ccatttgtac caacaaagtg aatagtcgac acttgaagct ggggattgtc
     9841 caagcgttcc aaaatccagt tgacacgtgc aacgccatgt tctaaatcag aggccgggcg
     9901 actagacaac cactgcctca tttctaaata attcatgttt ccctctctat aaaaagcaac
     9961 agggggatga cctgttgctt gatactgttt tatttaatgc ccattaactt ggccaattca
    10021 ggtatttttt gtaaacttgg taagacaagc atagtcacga caatccgtaa tggaatttca
    10081 aaaactgcct taatcaagcg actgctatac agaacaatcc aaggtgttcc aaaatggaaa
    10141 tggagggcaa tcggtgtcat gataaaggaa atcacaacct gaatcaagac aacaacccct
    10201 gcaacataga gccaatcagc cttgttcttg gtgtctagcg gttttcgata gaagaaccag
    10261 ccgtagataa atgccgacac cgcctcaatc aaaatccaag tgaagaggac cgtttgcccg
    10321 ctaaacaaga caaagactgg gtctgaaata gccgcaaaga cagctgccca aaccggacca
    10381 gcaatagctc ccaaaatagt attgggaacg aaggtaaact gaacctgtaa agtatcagaa
    10441 atccgaatag agaaatagtt ctccacaatc atgaccaagg caagggtcat cgcaatagca
    10501 gccaacagct gcaccgttag ttttggaatt ttcttttcca taaaaaatgc tcctttttca
    10561 aaaaatggaa ctgtcgtgtg tattcgacag tggatgcaat ggtttaccgc gctactctca
    10621 gccagatcca atgttcctac tctaggagac atcagataca agcaaagatc agcttcgtta
    10681 tgtggcaaca tcccatccac atacacttga cgcaaaccat ctactctgta caaatttctt
    10741 tgctagtcta ttgtatcaca cactatatat tttgaccact tgctaaaaac tatacctttc
    10801 taaaggtata aactataaga gaaactggca gtctccgact accagttagt ttttcttatt
    10861 tcaaacgttc aacttcacgc gcaataacca attcttcatc cgttggaata accaagacac
    10921 gaacttttga ttctggcgtt gaaatgtcac cattcgcacc acgaacattc ttagctgggt
    10981 ctatttcgac accgaaccaa gacaaacctt ctactacgtc attgcgcata agcggtgcat
    11041 tttcacccat acctgctgtg aagacaaggg catctgcacc attcaaaaca gcaaaatatt
    11101 gaccaatgaa tttcttgata cggtcgataa aaatgttata agccaaaaca gcatctgggt
    11161 tcttagcttc caaacctgat tcaatgtcac gcatatcact tgaataaccc gatacaccaa
    11221 gcaaacctga ttgtttattc agcatgttaa caacatccgc agcatccttc aactcatcaa
    11281 catttgcaat caagtaagga ataatcgctg gatcaatatc tcccgaacga gttcccatca
    11341 ttggaccagc tagaggagtg aagcccatag aagtgtcaac tgattgaccg tggtagttgg
    11401 cagtaataga tacaccgttt ccgatgtgag ccgtaatcaa ttttaattcc tcaatcggac
    11461 gtcccaatac ttttgctgcc tcatgcgcta cataatagtg actagttccg tgagccccat
    11521 acttacgaac cttgtggtcg gtatagtact tttgtggaat cggataaaga taggcatgct
    11581 ttggcatcgt agtgtgaaag gctgtatcaa aaacagctac attcaaaact tctggtagta
    11641 cttcctcaaa tgcacgcatt gctgctacgt gtgctgggtt atgaagcggt gccaaagcac
    11701 ttagttcttc aatttgctca acctcttttg gtccaataac agctgattct ttgaaaatct
    11761 ctccacctgc aacaacacgg tgtccgatac cagtaatttc ctcgtaagca gcgatgatgt
    11821 tgtgtttaag caagtcttct aggagaatct taaccgcaac cgtatgatta ggaatatcta
    11881 aaacttcact ttctttcttc ccatcatact taactgttga gatggaatcg ttcaaaccga
    11941 tgcgttcaac aatccctgcc gctaatactg tttcttctgg catttgataa agttgccatt
    12001 ttagacttga actacctgca ttaattgcaa ttgtttttga catatcttac ctcttttaaa
    12061 gcgttttcct aactagtata acagattttc acttaaattg cattctcttg cttccaattt
    12121 ttgaaattga gcatgaactt ctgaatagct tctggttctt gaagactttg taagggataa
    12181 acaaacggcg tcaatgatct tgcagctttc ttttgcaaga caaaaataga cttggccata
    12241 gcagccttcc caaagagatt tggtggtagg gcaatcatgg caacaatatt ggcttgctcc
    12301 tgtaaccaac ctttcagcaa atcgctttgc ggactagtca ataaatcatt tggagccaac
    12361 aaaatcgcaa aaccattttt ttccagatat ttgagggatt gttccatgag taaatgatgg
    12421 gcgtaggtat gttcatttgg gctggcgacc tgatagcggc tagcaatctg gtcatctgga
    12481 tagtagccaa taggcaaatc tcccaaaatt acttgacttt ccttcaaaat ctgcggacgt
    12541 accgcatctc cctgagcaaa agaaatatct gcctgcatga catccgcaat acttgccgac
    12601 aaatcaatca agaggtcgtc cacttcgatc cccaagtaat ccaattcttt ctggctggcg
    12661 ttgagaatgg tctgagctag attgcctgtc cccgaaccaa tttccagaac cgtcaccttt
    12721 tgagtcggca ccaattggtc tactagaaaa gataggataa atccgataga gtctggtgtg
    12781 aactggtgat tatactgcat gggttcagtc tgattggcct tgatgagcaa gaattgatag
    12841 gcacgacgcc attcttcctt ggttaaatct agttgtttca aggtcttgtt attattgaca
    12901 acaaggtccg tctcatgctg attagctacg taagcagcat tttgctcaat catggcatca
    12961 tagatattgg tacctagctg gttttggata gtctgtacgt tttctaatag caggtcgtaa
    13021 gcctgttcga tcttttcaaa attcatactc ctatcttatc aaaatttcac agactttgca
    13081 ccaaaaagga gagcgagtca actcaccctc cttctgatga tagccccaaa ggaaattgat
    13141 aggtatacag accatcactt gttaactcaa ctgtaaccaa tagttggtct cctttaagtt
    13201 gataacgagc ctgtccctct tgaaaggaga cccttcctct attctccttc tgcttagaac
    13261 ctgtggttaa atttgcttcc ttttcattca ctaccgctcc cgtaactatt ttttctactt
    13321 gcaggtcctg ctcgacctta tccaagacca tctgagccat gatgtaggca gtcgtttctt
    13381 gcctggccac ctgaaccaag cgactttcgg cttcttggtg gtggagataa aactggagca
    13441 agagactaaa aactgccagc atcaagaggg cgtagagcag gatgccagcc ttaacttttt
    13501 tctttaaaag catagataaa ggtccgctcc tctccgtttt taaagctaaa gtcgatacgc
    13561 accagctggt tttcttggga aatagctgca ctgtccacct gatagagcat gggctggtag
    13621 ccctgtcccc tgtcatttgt tttccgaaaa tcatccgatc gtgacttacc aaaggccaac
    13681 gactgaccac ctttatcaac atagaccttg ccattttcaa ccttgaccaa ttccgaccgg
    13741 tcccactctg accgcaactg actagaaaat accagccact ccttttggac agattgacgt
    13801 tggtaatgtg cttcttgaat caacaacttt gacaagcctt caaagaccag aagacttcct
    13861 gataggatga ctaaggctac caagcattcc aggagagtaa aagcgggaat tttacttttt
    13921 aacaaccgat aaaacctcct tcccctcatg gaaaaccgca accctgtccg ccgttcgttt
    13981 cacctgaact gtaacaccgt ttaaggctaa gttatcttgc tcggtctgga cagccatctg
    14041 agctagattg agaacctcct gttgttccaa ttcccaagcc tgtcgcctac gacctgcatc
    14101 caccgctgac aaaatcaagc tgcaaatggt taccagggtc gcaagggcaa ccaaactttc
    14161 tagcaaaata taagccttat tcttctgttt tcttatagcg accactccct atatataatt
    14221 gatacgtgac tgtctttcga ctaagccgaa agcgtatctt ggtcagagaa gaatttccac
    14281 catcttcttc aaatgtgatg gtcttcccct ctaatacttc tacatttctg ggtacctgaa
    14341 ctgcctgacg accatttgcc acttcttgtt cgccgatagt caagctaact gactggtgag
    14401 aactagcagc caatttctga ctgtctttat agatggcctc aaattcccag agaaaaattt
    14461 cttcctgaac agaccgaaag accgtttgta ctgttcctga tagggaaaca gctaaaaaac
    14521 tgacaacaaa caaggtcagc aggctttcaa acagggtaaa agccttattt tttcgccgta
    14581 gcatactgtt ttgcttgttt ttcagtaata taacctttcg cctgcaaatc tgccaccgtt
    14641 gcttcctcgt catgttccaa ttcatagagc tccatctgac tctccactac tttgacaaca
    14701 gccgcatccc ccttcttctg aatagcatcc ttctgctggc tcaaatttgg cacaaaaagg
    14761 agcaagagca ggctaatgat ccccaaaacg actaacattt ccaccagagt gaaccctttt
    14821 accttcattt caactaattt tttcatttta caactccata ttttgataaa tcggcagcaa
    14881 catggctgcg tagatgagaa cgaccattaa ggccacaaag agaaagacca gcggttggat
    14941 tagctgcatg gctctattga cccgagagaa aaaatcctcc caacattcag ccgcataaac
    15001 tgtcaactcg ctacccaact tggacttgac ctgaccgtac tcgacaatca aactcagctc
    15061 ccgcttaaaa aaggcgtagg tcttaatgtg ttcgtgaaag ctctgaccat tggaaagcga
    15121 ctgctccagg tcctggccaa tctcccgaaa aagctgcgac tgctgctctt gcatcaagcc
    15181 cacaatctgc ggcaggtcca agccttgccc aatcaaactc ccccactccc tggcgtaata
    15241 ggccgtcagg taggtttgga tgagttttcc gaaaaatggc agagctgcca agcaggaaaa
    15301 gaccttaatt ttattggttt tgcgaaacca aaccatacca gctaagaccg ccaccaaact
    15361 aagtccactt agagataaaa agatggtcgg tagatgatta atcagcactg tcgctgcatt
    15421 accttcctcc aactggggca gaagatagtt tttcaaaccc agcataatca agagcagaaa
    15481 acctagcaag ataactggat aggtcgccac ctcaatcagc tttttacgaa ccttgctgac
    15541 attttccaga taggactgga tatggctcaa actcaggctg gtattaccat gaacttctgc
    15601 cagagccacc tgtgtgacca ccgcatctga aaaccgcaaa tctgccagta aactcgaaaa
    15661 gggtttgcct gccagcaacc cgtctgacaa gacctgggta tagagatctg ccagaagctg
    15721 actgcgtttg aggaaatcaa caatctcccc cagatgaaaa ccactagcaa aaagattatt
    15781 gaaaagctca atgaccttac gctggcgagc caagggcaat tttttctgtt tctgcctgcc
    15841 taagactgat atgtcctgct gcaaaaaggc gatcaatttg cgcattccac tggtctgcgg
    15901 aatggttttg gtaatttccc tttgcaaaat ctaccacacc tcctcccctg attaaacgtt
    15961 gataggcaat accttgaagg gcattgttta actcctcagg actgaccccc aattccaaca
    16021 tacgagcata gacacccgaa atagaccttg cgtgaaccgt tgagaagacc gtcgctcctg
    16081 tcagactggc tcgaatgact gctcgagcgg tttccgcatc ccgaatttca ccgatgatga
    16141 gcaagtctgg tcgatgacgg agggacagtt tgatcagatt gtcgtaggtg gctccgatgg
    16201 cttcattgag ctggagttgc agcatgtcct cctgtttgat ttcgacagga tcctcgatgg
    16261 tcaaaatctg cttgtctggg aatttcagcc tggcaagatg gtacataagt gtggtcttac
    16321 cagagccgac tggacccgaa aaaaggtaga gccctcgtcc cttgatttct tctgcaagcc
    16381 gctcagccgc ctcaaaccag aacttgagtt ccttgtcatt gtcatagagc aagcggatca
    16441 ccagactttc cttgccacga taatctccga cagttgataa gcgaagtgaa atctctccgc
    16501 taccataatc atagtcacag gaaccctgct ggctacggcg tttttcacca acatttaaac
    16561 ctgctaaaaa cttgaaatgg ctgatgatgg ctgttacttc ctcctcagcc aagtcttgca
    16621 caaactcccg ttcatccatg atgcgatgat aaacttgata gctttccccg cgaggaacca
    16681 gataaatgtc actgacccta tccgccactg ccttttcaat caactttctt gctttttctt
    16741 gaatcataaa acctcctcac cctatctatt cgcaaggaaa cttgattttc catacttttt
    16801 tgttaa
//
LOCUS       DBNFEG010000029        16733 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.029, whole genome shotgun sequence.
ACCESSION   DBNFEG010000029 DBNFEG010000000
VERSION     DBNFEG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16733
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.029"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            416..5311
                     /locus_tag="AC1PNG_000658"
     CDS             416..5311
                     /locus_tag="AC1PNG_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0018262 - isopeptide cross-linking
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SspB-related isopeptide-forming adhesin"
                     /protein_id="HIP6701179.1"
                     /translation="MTKTCNHHFLVNQEKGEKHVFRKSKKYRTLCSVALGTMVTAVVA
                     WGGTVAHADEVSSSVDTTIQRTENPATNLPEAQPNPVSEQTESLGSTGQSNGTIAVTV
                     PHDTVTQAVEEAKAEGVSTVEDSPMDLGNTRSATETSQQISKAEADAQNQVVAINEVT
                     ETYKADKATYESNKARIEQENKVLSQAYEGANQTGKETNTWVDTKVNDLKSQYADADV
                     TLIEQVVSAGNGTSVLDYTNYGKTVETIQSTNEQAVADYLTKKTKADDIVAKNQAIQK
                     ENEAGLAKAKADNEAIERRNQAGQAAVDAENRAGQAAVDQANQEKQQLVSDRAAEIEA
                     ITKRNKEKEAAVRKENEAIDAYNTKELERYQRDLAEISKGEEGYISEALAQALNLNNG
                     EPQAQHGAITRNPNQIISTGDTMLGGYSRILDSTGFFVYDSFKTGETLSFNYQNLQNA
                     RFDGKKISRVTYDITNLVSPAGTDAVKLVVPNDPTEGFIAYRNDGNGDWRTDKMEFRV
                     VAKYFLEDGSQVTFSKEKPGVFTHSSLNHNDIGLEYVKDSSGKFVPINGSTVQVTNEG
                     LARSLGSNRASDLNLPEEWDTTSSRYAYKGAIVSTVTSGNTYTVTFGQGDMPQNVGLS
                     YWFALNTLPVARTVTPYSPKPHVTVELEPIPEPITVTPDVFSPKTFTPEKPVTFTPKP
                     LEEVVQPSLSLTKVTLPVKPIPKELPTPPQVPTVHYHAYRLTTTPEIMKEVVNSDQAN
                     LHEKTVAKDSTVIYPLTVDAISPNRAQTTSLIFEDYLPAGYLFDKETTQKENGNYVLS
                     FDETKNFVTLTAKENLLQEVNKDLTKVYQLNAPKLYGSVQNDGATYSNSYKLLLNKGT
                     TNAYTVTSNVVTVRTPGDGETTTLITPDKNNENADGVLINDTVVALGTTNHYRLIWDL
                     DQYKGDRSAKETIARGFFFVDDYPEEVLDVVENGTAVTTLDGQKVSGITAKNYASLNE
                     APKDLQDKLARAKITPTGAFQVFLPDDNQAFYDQYVQTGISLALLTKMTVKDSLYGQT
                     KTYTNKAYQVDFGNGYETKEVTNTLVSPEPKKQNLNKDKVDINGKPMLVGTQNHYTLS
                     WDLDQYRGIKADNSQIAQGFYFVDDYPEEALLPDEAGIQFVTSDGKTVSGITVKAYSQ
                     LSEAPKTLQAALSKQKIQPKGAFQVFMPEDPQAFFESYVTKGENITIVTPMTVLETML
                     NSGKSYENVAYQVDFGQAYETNTVTNFVPKVTPHKSNTNQEGISIDGKTVLPNTVNYY
                     KFVLDYSQYKDMVVTDDVLAKGFYMVDDYPEEALTLNPDGIQVLDKAGNRVSGISVST
                     YASLSEAPEVVQDAMAKRQFTPKGAIQVLSSDDPKTFYETYVKTGQTLVVTLPMTVKN
                     ELTKTGGQYENTAYQIDFGLAYVTETVVNNVPKLDPQKDVVIDLSHKDASLDGKEVAL
                     HQTFNYRLVGALIPSNRATALFEYGFEDNYDEKHDEYNGVYRSYLMTDVILKDGSVLK
                     EGTEVTKYTLQQVDTENGLVLISFDKSFLETISDDSAFQADVYLQMKRIAAGQVENTY
                     LHTVNGYVISSNTVVTHTPQPEEPSPNQPTPPQPPIETIEPPVPASVLPNTGEQETLL
                     GLIGAGILLGTAYGLKKKEEK"
     gene            5312..5503
                     /locus_tag="AC1PNG_000659"
     CDS             5312..5503
                     /locus_tag="AC1PNG_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIP6701180.1"
                     /translation="MNPKTIYEKDSDQDGLTDAQELALGTNPQSVDTDGDGQADLEEL
                     QSGHSPLVPQKELCDGLEL"
     gene            5487..6038
                     /locus_tag="AC1PNG_000660"
     CDS             5487..6038
                     /locus_tag="AC1PNG_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701181.1"
                     /translation="MAWNFDTMKEALSEMEKVDYQEFIKAFLSLELSISDRTILNQVY
                     QDYMDEDDLSLISDELRVKVDSYQDEVQVDMTDILEKLYRTGEGSSFIMDLMSSNSLS
                     DTLEQYEVLDSDDYSPLSFETLQAMIQQELAISSQDYFGDLVHLALQKDLLDQKSHFL
                     QHYVATVMEGIPQESDQRALVLD"
     gene            6087..12911
                     /locus_tag="AC1PNG_000661"
     CDS             6087..12911
                     /locus_tag="AC1PNG_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HIP6701182.1"
                     /translation="MNQEVLLQMMRATIPRDRALLEAFLYYQAEHFDEEWDSLIRQFM
                     TNRQGIKRSVQVLHFETDVSAFVQASPYDTAHDLLTYTQVFGQTGLQKLDKLSPSEKD
                     LVIEVALFNLATRFQLLDSNGHYQTISPDSLLQKSRGANLVNVYRVANNLADRISRDI
                     EQFLLTYEPELETRADETVLENEETVDEHKVSSNQAISFREDGSLIIASLDVDLSQLN
                     VQTGKTSHLPAYEELSLRRKFEILTYFDQIRNERSKVPSFRRGDFDTEMEMTPIFEGE
                     ELLTYLEADGSPNELKRTLTTVEEKELEKIGQAIRIENQEKLTQVGIDLSQFEPDQVG
                     ILLDAAGRFRLKNSDLALLGGYPKASVTQLALATELLQMGLSHEKVEFFFGSQLSLEE
                     LRQVAYAFLHQELSREDAEQFEMDKGNQPDLTFRDWKSKLEKAESKVVVDEELAENPL
                     VQRVLDTYPLGSLVSYKGQDFEVVSVSDARLNGLIRIELVNDFSDIIEQNPVLYVRTW
                     EEVSQALHQPKAEPQTELEEADQELNLFSFLEEEPVQSIGLLEPDGSEKGQNDTDLEE
                     TDSQIPEEVVVETIPEIPVTDFYFPEDMTDFYPKTARDKVETNIAAIRLVKNLEVEHR
                     NASSSEQELLAKYVGWGGLANDFFDDYNPKFSKEREELKSLVTDKEYSDMKQSSLTAY
                     YTAPALIRQMWDKLERDGFTGGKILDPSMGTGNFFAAMPKHLREKSELYGVELDTITG
                     AIAKHLHPNSHIEIKGFETVAFNDNSFDLVISNVPFANIRIADNRYDRPYMIHDYFVK
                     KSLDLVHDGGQVAIISSTGTMDKRTENILQDIRETTEFLGGVRLPDSAFKAIAGTSVT
                     TDMLFFQKHLDKGYVADDLAFSGSIRYEKDSRIWLNPYFDREYNSQVLGTYEVRNFNG
                     GTLSVKGTSDNLIASVEAALHQVKAPREIDRNEVIINPDVLTKQVIDTSIPAEMRENL
                     GQYSFGYQGTMIYYRDNKGIRVGTKTEEISYYVDEEGNFKAWDTKHSQKQIDRFKSLE
                     VTDSTALDVYVTDDAAKRGQFKGYYKKTVFYEAPLSDKEVARIKGMVDIRNAYQEVIA
                     IQRHYDYDKGTFNHLLGKLNRTYDSFVKRYGYLNSAVNRNLFDSDDKYSLLASLEDES
                     LDPSGKSVIYTKSLAFEKALVRPEKEVKKVHTALDALNSSLADGRGVDFAYMMSIYQV
                     ESQMTLIEELGDLIMPDPEKYLNGELTYVSRQDFLSGDVVTKSEVVDLFVKQDNQDFN
                     WSHYAGLLEAIKPARITLADIDYRIGSRWIPLSVYGKFAQETFMGKAYELSDQEVATV
                     LEVSPIDGVITYQSKFAYTYSNATDRSLGVPASRYDSGRKIFENLLNSNQPTITKQVV
                     EGDKKKNVTDVEKTTVLRAKETHLQELFQGFVAKYPEVQQMIEDTYNRLYNRTVSKSY
                     DGSHLTIDGLAQNISLRPHQKNAIQRIVEEKRALLAHEVGSGKTLTMLGAGFKLKELG
                     MVHKPLYVVPSSLTAQFGQEIMKFFPTKKVYVTTKKDFAKAKRKQFVSRIITGDYDTI
                     VIGDSQFEKIPMSREKQVTYINDKLEQLREIKLGSDSDYTVKEAERSIKGLEHQLEEL
                     QKLERDTFIEFENLGIDFLFVDEAHHFKNIRPITGLGNVAGITNTTSKKNVDMEMKVR
                     QVQAEHGDRNVVFATGTPVSNSISELFTMMNYIQPDVLERYQVSNFDSWVGAFGNIEN
                     SMELAPTGDKYQPKKRFKKFVNLPELMRIYKETADIQTSDMLDLPVPEAKIIAVESEL
                     TQAQKYYLEELVERSDAIKSGSVDPSRDNMLKITGEARKLAIDMRLIDLAYSLSDNQK
                     ILQVVDNVERIYREGAEYKATQMIFTDIGTPKSKEEGFDVYNELKALLVDRGIPKEEI
                     AFVHDANTDEKKNSLSRKVNSGEVRILMASTEKGGTGLNVQSRMKAVHHLDVPWRPSD
                     IVQRNGRLIRQGNMHQEVDIYHYITKGSFDNYLWQTQENKLKYITQIMTSKDPVRSAE
                     DIDEQTMTASDFKALATGNPYLKLKMELENELTVLENQKRAFNRSKDEYRHAISYCEK
                     HLPIMEKRLSQYDKDIAQSLATKSQDFVMRFDNQKMDNPTEAGDYLRKLITYNRSETK
                     EVRTLASFRGFDLKMTTRGPSEPLPETVSLMIVGDNQYTVALDLKSDVGTIQRISNAI
                     DHILDDQEKTQELVKDLKDKLQVAKVEVEKIFPKEEEYQLVKAKYDVLAPLVEKEAEI
                     EEIDAALAKFSEDTTAQKKQQLALEI"
     gene            12982..13281
                     /locus_tag="AC1PNG_000662"
     CDS             12982..13281
                     /locus_tag="AC1PNG_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5962 family protein"
                     /protein_id="HIP6701183.1"
                     /translation="MTKTVEEMRYQLEEWLSQGFTSSEDRANYQTLKEQYEDETLDYS
                     FSKREITGQLELIITTRENDFPSLDEVTKAEYLDLVAQLDDLDKRQADYYRKQLA"
     gene            13295..13585
                     /locus_tag="AC1PNG_000663"
     CDS             13295..13585
                     /locus_tag="AC1PNG_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5966 family protein"
                     /protein_id="HIP6701184.1"
                     /translation="MLEQILQSLLIIAAIGLMLLVLYRIVKVSGALFLIGLISGLVFI
                     EIYGVYLFFTERYLYSEDLATNGVWSFTGFFIALNLFLVLSFIIKWWRNRIV"
     gene            13681..14319
                     /locus_tag="AC1PNG_000664"
     CDS             13681..14319
                     /locus_tag="AC1PNG_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIP6701185.1"
                     /translation="MLNKVKARFLIGVGGLIAVSFMVMIGYTIGSQSVSKQTEHQIRA
                     EANKLVTKEKQEERATVLSDELVKEFLTQYFTKVQLGENNTRIKPYMTDSAFSEEETN
                     QNKAINQVYKDYMLDYRFESASIFVNTESNVALAEVSYQVTYVSDLSEQQQRTTQTET
                     KTVMLSYSKVSDKLLVNQLTIWNGKLEDMKEATDGANSSIPTIQGTTTSENN"
     gene            14358..15434
                     /locus_tag="AC1PNG_000665"
     CDS             14358..15434
                     /locus_tag="AC1PNG_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIP6701186.1"
                     /translation="MTRIKAVKQKAILNVAESLGYSFRRLSGHIYEHPDHDSFRIFAD
                     TNTFKWFSRDIQGDVIDFVQLVAGVTFKEAVSYLETGDFEQAKLIEETYQPFQYYLHE
                     EPFQQARIYLKDIRGLSDQTINTFGRQGLLAQATYQSEPVLVLKSYDHNGTLQAASLQ
                     GLVKNEEKHDRGYLKKIMKGSHGYVGISFDIGNPKRLIFCESVIDMMSYYQIYQKQLS
                     DVRLISMEGLKLSVIAYQTLRLAAEEQGKLAFLDTVNPSRLSHYLQAIQETTTFFQTH
                     SNALTLAVDNDEAGREFCQKLSDKGLPLSQDLPPLQGLETKLDWNDIVKRQKELSLRD
                     LIQSAQTKVIRDHPPPKREHTFEL"
     gene            15488..15715
                     /locus_tag="AC1PNG_000666"
     CDS             15488..15715
                     /locus_tag="AC1PNG_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5965 family protein"
                     /protein_id="HIP6701187.1"
                     /translation="MSVIERLAEKVARQEEKVSRETEKLEHYRDQLQTAMYSTFIKRQ
                     QSSQLSFHEALEQAFGKETTLHPNYRNEDTE"
     gene            15712..16101
                     /locus_tag="AC1PNG_000667"
     CDS             15712..16101
                     /locus_tag="AC1PNG_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5945 family protein"
                     /protein_id="HIP6701188.1"
                     /translation="MSKTWNFDQPLDDVKPTSSHEERAKIAALFHKQDEKPIEEVDYV
                     AAFEQQQKESESKDVQTPESKVKQSQPKKVNITSDYKQHLADTIAQNNKDISACQKQI
                     EELHQLIDEKKIQNKKLQAISVAIDDL"
     gene            16151..16447
                     /locus_tag="AC1PNG_000668"
     CDS             16151..16447
                     /locus_tag="AC1PNG_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701189.1"
                     /translation="MKFFQKKKQNQDQFKRLIHRLSEMSDTELTKVEQLLDVVFDTNF
                     KPDRNVELSQSVITDEEQSLDKSIQEAKNKLNTEQLEKNIERFRQGRRESNNNK"
BASE COUNT         5555 a         3167 c         3555 g         4456 t
ORIGIN      
        1 tcacatgaac aaggaaatca atactttcca agaagtctta aaatcaacga caccttaatt
       61 acaaattgta gagaaataga gctgaaactt gttgacaaga aacgctttaa gactgctaaa
      121 gttagaaagg ttaattggcc gtcttaatgt aaaaattgat atgctgataa tcaggtcatc
      181 cacttttatg ggtggctttt ttcttttccc tcaatttatt gtgataacaa aatcaagcct
      241 tcttttttat gatggtattg aacttgggct acgagctgta caaaaaagag agtttctcct
      301 agaacggaaa gttcttcgtc aaaactccta tttttgtcgt gctcgcttaa cgccctcgta
      361 tctaataaag agtgacagtt tgttgctact tatcaaaaga aaagaaggac tatttatgac
      421 caaaacatgt aatcatcact ttcttgtcaa tcaggaaaaa ggcgagaaac acgtctttcg
      481 caagagtaaa aaatatcgca ctctatgttc cgttgccctt ggaaccatgg tgacggctgt
      541 tgttgcttgg ggaggaacgg ttgcacatgc tgatgaagtc tcatcatcag ttgacaccac
      601 cattcaacga acggagaatc cagccacaaa tttaccagaa gcacagccaa accctgtatc
      661 tgaacaaact gaaagtttag ggtcaactgg acaatctaac ggtacaattg cagtcaccgt
      721 accacatgat acggtaacac aagcagttga agaggcaaag gctgaaggtg tttctacggt
      781 tgaagatagt ccaatggatt taggaaatac aagatctgcg acagagacca gccaacaaat
      841 ttcaaaagca gaagcagatg cccaaaacca agttgtggct atcaatgaag ttactgaaac
      901 ctacaaagct gacaaagcga catacgaatc aaataaagca cgcattgaac aggaaaataa
      961 ggtgctgtca caggcctacg aaggggccaa ccaaactggt aaagagacaa atacttgggt
     1021 tgataccaaa gtcaatgacc taaagtccca gtatgcagat gctgatgtga cattaattga
     1081 acaagtagtt tcagcaggaa atgggacatc tgtccttgac tatacaaact atggcaagac
     1141 tgttgaaacc attcaatcaa ctaacgaaca agctgtagcg gattatctaa caaagaaaac
     1201 taaggcagat gatattgttg cgaaaaatca ggccattcaa aaagaaaatg aagctggact
     1261 tgctaaggca aaggcagata atgaagccat tgaaaggcgt aatcaggctg gtcaagcagc
     1321 tgttgatgct gaaaaccgtg caggtcaagc cgcagtagat caagcgaatc aggagaaaca
     1381 acaattagtt tcagatcgag cagctgagat tgaagctatt acaaaacgca ataaagaaaa
     1441 agaagccgca gtcagaaaag agaatgaagc gattgatgcc tataatacca aagaattgga
     1501 acgctatcaa cgtgacttag ccgagatttc aaaaggtgaa gaaggttata tttctgaagc
     1561 ccttgctcaa gccctcaatc tcaataacgg tgagccacaa gcacaacatg gagcaattac
     1621 ccgaaatcct aatcaaatca tttcaactgg cgataccatg ctgggtggct actcaagaat
     1681 attggattca acaggattct ttgtctatga tagcttcaaa acaggtgaga cccttagttt
     1741 taactatcaa aatcttcaaa atgcacgatt tgatggtaaa aagattagtc gagtgactta
     1801 cgatattacc aaccttgtat caccagctgg aaccgatgct gtgaaattgg ttgtaccaaa
     1861 cgatcccaca gagggcttta ttgcctatcg aaatgacgga aacggtgatt ggcgaacaga
     1921 caagatggag tttcgtgtag ttgccaagta tttcttggaa gacggttcac aagttacctt
     1981 ctccaaagaa aaaccaggtg tcttcacgca ttcttccctc aatcataatg acattggtct
     2041 agaatatgtc aaagattcat ctgggaaatt tgtgccgatt aatggttcta ccgttcaagt
     2101 gactaatgaa ggtctggcac gttctttggg ttccaaccgt gcaagtgatt tgaatttacc
     2161 tgaggaatgg gataccacat caagtcgtta tgcttataaa ggagctattg tctcaacagt
     2221 tacatcaggt aatacctaca cagtaacctt tggccaaggc gatatgccac agaatgttgg
     2281 cttgtcatac tggttcgcct taaataccct accagttgca cgtacagtaa caccgtatag
     2341 tcccaaacct catgtaacgg tggaacttga acccattcca gaacctatta cggtaacacc
     2401 agatgtcttt tctcctaaaa catttacacc agaaaagcct gtgaccttca cgccaaagcc
     2461 tttggaagaa gtagtgcagc ctagtctgtc cttgaccaaa gtaaccttac ctgtcaaacc
     2521 tatcccaaaa gaacttccaa cgccaccaca agtaccgaca gtccattatc atgcgtaccg
     2581 tttgacgaca actccagaga ttatgaaaga agtggtcaat agtgaccaag ctaatcttca
     2641 tgagaaaact gtcgcaaaag attcaactgt gatttatccc ttaacagttg atgctatatc
     2701 gcccaatcgt gcccaaacga ctagtctcat ttttgaggac tacttgcctg ctggttacct
     2761 atttgataag gaaacaacac aaaaagagaa tggaaactat gtccttagct ttgatgagac
     2821 taagaatttt gtgaccttga ccgcaaagga aaacttgttg caggaggtaa ataaagattt
     2881 aaccaaggtt tatcaactga acgctccaaa actctatggt tctgttcaaa atgatggggc
     2941 aacctattcc aatagttaca aactcctttt gaacaagggt acaaccaatg cttacacagt
     3001 cacttcaaat gttgtaacgg ttcgtacacc aggtgatggg gagacaacca cactcattac
     3061 accagataaa aacaacgaaa atgcggacgg tgtcctcatt aatgacacgg tcgtagccct
     3121 tggcacaacc aaccactacc gattgatttg ggatttggac cagtataagg gagatcgttc
     3181 tgctaaagag acaattgcac gaggcttctt ctttgtggac gattacccag aggaagtgct
     3241 cgatgtggtg gaaaatggca cggctgttac aacccttgac ggtcaaaagg tatcaggaat
     3301 aacggctaaa aactatgctt cactaaatga agctcctaaa gaccttcaag ataaattagc
     3361 tcgtgctaag attacaccga caggtgcctt tcaagtcttt ttgccggatg acaaccaagc
     3421 cttttatgac cagtatgttc aaacaggaat ttctttagct cttttgacca aaatgacggt
     3481 gaaagatagt ctctatggtc aaactaagac ttatacaaac aaggcttatc aagttgattt
     3541 tggaaatggc tatgaaacca aggaagtgac caacaccctt gtttctccag aacccaaaaa
     3601 acagaacctc aataaagaca aagtggacat caatgggaag cccatgctag tgggaactca
     3661 aaatcactat actctctcat gggacttgga ccaataccga gggattaaag cagacaactc
     3721 tcagattgca caaggttttt actttgtgga tgattatcca gaagaagctt tattgccgga
     3781 tgaagcaggt attcagtttg tcacatctga tggcaaaaca gtttcaggaa tcacggtgaa
     3841 ggcttattct caattatcag aagctcctaa aacgctacaa gcagcccttt cgaaacaaaa
     3901 aattcagcct aaaggagctt ttcaagtttt tatgcctgag gacccacaag ccttttttga
     3961 atcttatgtg accaaggggg agaatattac cattgtcact ccgatgacgg ttttggaaac
     4021 catgcttaat tcagggaagt cttatgaaaa cgtggcttat caggtggact ttgggcaagc
     4081 ctatgaaacc aacacggtga ccaattttgt ccctaaagta actccacata agtctaatac
     4141 caatcaagaa ggtatttcaa ttgatggaaa gactgttctt ccgaatacgg tcaattatta
     4201 caaatttgtc ttggattaca gtcaatacaa ggacatggtc gtgacggatg atgttcttgc
     4261 caagggattt tacatggtag acgattaccc agaagaagcc cttaccctaa atcctgatgg
     4321 cattcaagtt ttggataagg ctggcaatcg tgtatctggt atctctgtca gcacctacgc
     4381 tagtttgtca gaagctccgg aagtcgtcca agatgccatg gctaaacgtc agtttacacc
     4441 taaaggagcc attcaggtcc ttagtagcga tgatcccaaa accttttatg agacctatgt
     4501 gaagactggt caaaccttag ttgtcacgct tccgatgacg gtaaaaaatg agttgaccaa
     4561 aacaggtggt cagtatgaaa atacagccta tcagattgat tttggcttgg cctatgtcac
     4621 ggaaacagtg gttaataatg ttccaaaatt agacccacaa aaagatgtgg tgattgactt
     4681 gtctcataaa gatgccagcc ttgacgggaa agaagtggcc ttgcatcaaa cctttaacta
     4741 tcgcttggtt ggagcattga ttccaagcaa tcgtgcgact gctttatttg aatatggttt
     4801 tgaagataac tatgatgaaa agcatgatga gtataatggt gtttatcgta gctatctgat
     4861 gacggatgtc atcctcaaag acggttctgt cttaaaagaa ggaacggagg ttacgaaata
     4921 taccttgcaa caggtagata cagaaaatgg cctcgtgtta atttcatttg ataaatcctt
     4981 cttagagact atctctgatg attcagcttt tcaggcagat gtttaccttc aaatgaaacg
     5041 gattgcggct ggtcaggtgg agaataccta tcttcataca gtaaatggct atgtcatcag
     5101 ttcaaataca gttgtaacac atacacctca acctgaagaa ccaagtccaa atcaacccac
     5161 accacctcaa ccaccaattg agactataga accacctgtt ccagctagtg ttttgccaaa
     5221 tacaggggaa caggaaaccc ttttgggctt gattggagcc ggtattctac ttggtacagc
     5281 ttatggacta aagaaaaagg aggagaagta gatgaatcca aaaactattt atgaaaagga
     5341 ttcagaccaa gatggtttga cagatgctca ggaactagct ttgggaacca atccgcagtc
     5401 tgttgacaca gatggtgatg gtcaagctga tttagaagag ctacaatctg gacattcacc
     5461 actagtccca caaaaggagt tgtgtgatgg cttggaactt tgacaccatg aaagaggctt
     5521 tgtctgaaat ggagaaggtc gattaccaag agtttatcaa agcctttctc tctttggaat
     5581 taagtatttc tgatagaaca atcctcaatc aggtttatca agattatatg gatgaagatg
     5641 acctatcctt gattagtgat gagctacgag ttaaagttga tagttatcag gatgaagtac
     5701 aggtagatat gactgacatt ctggaaaagt tgtaccgaac aggtgaaggt tctagtttta
     5761 ttatggattt aatgtcctca aatagtctct cagatacctt ggaacagtat gaggttttgg
     5821 atagtgacga ttattcacca cttagctttg aaaccttaca ggctatgatt caacaggaat
     5881 tggctatttc cagtcaggat tattttggag atttggttca ccttgccttg caaaaagatt
     5941 tactagacca gaaaagtcat ttcttacaac actatgtggc aactgtaatg gaaggtattc
     6001 cacaagaaag tgaccaacga gccttggtcc tagattaaca caaagggctg agaaacactc
     6061 tcagcctttt taaaatggga gggaaaatga atcaagaagt cttactacaa atgatgagag
     6121 ccaccattcc tcgtgataga gccttgcttg aggcgttttt atattaccaa gcagagcatt
     6181 ttgatgagga gtgggatagt cttattcgtc agtttatgac caataggcag ggaatcaaaa
     6241 gatctgttca ggtacttcac tttgagacag atgtttcagc ttttgtccag gctagtcctt
     6301 atgatactgc tcatgatcta ttgacctata cacaagtttt cggccaaact ggtctccaaa
     6361 aactcgacaa actatcgccg tctgaaaaag acttggtgat tgaagtggcc ttgttcaatc
     6421 tggccactcg ttttcaacta ttagactcaa atggacacta ccaaactatt tcaccagatt
     6481 ctctattaca aaagagtagg ggagctaatt tggtcaatgt gtatcgtgtg gctaataatt
     6541 tagcggatcg gattagccga gatattgaac agtttctctt gacttacgag cctgagcttg
     6601 aaacaagagc tgatgaaact gttctagaaa atgaagaaac tgttgatgag cacaaagtga
     6661 gtagtaatca agcaatatca tttagagaag atggctctct catcattgct agtttggatg
     6721 tagatttgtc tcaactgaat gttcaaacag gaaaaaccag tcatctgcct gcctatgaag
     6781 agttatcttt acgacgtaaa tttgagatac taacatattt tgaccaaatt cgaaatgaac
     6841 gttccaaagt cccaagtttt agacgaggtg attttgacac agagatggaa atgacaccaa
     6901 tctttgaggg cgaggaatta cttacttatc tagaagctga tggcagtccc aatgagctga
     6961 aacgaacgct gaccacagtc gaagaaaagg aattagaaaa aattggacaa gccattagga
     7021 tagaaaatca agaaaaattg actcaggtgg ggattgattt atctcagttt gaaccagacc
     7081 aagtcggtat tttattggat gcggcaggtc gttttcgttt gaaaaattcg gaccttgctt
     7141 tactaggtgg ctatcccaaa gcctcggtaa ctcaactagc ccttgcgaca gaactactcc
     7201 aaatgggatt gagtcatgaa aaggttgaat ttttctttgg tagccagctt tcccttgaag
     7261 agctacgaca agttgcctac gcctttttac accaagaact cagcagagaa gatgcggaac
     7321 aatttgaaat ggataagggc aatcagccag atttaacctt cagagattgg aaaagcaagc
     7381 tagagaaagc tgagtcgaaa gtagtggttg atgaagagtt agcggaaaat ccactagtcc
     7441 agagagtatt ggacacttat cctctggggt cattggtttc ctataaggga caggactttg
     7501 aggtcgtgtc ggtcagcgat gctagattga atggtttgat tcggattgag ttagtcaatg
     7561 atttttcgga tatcattgaa caaaatccag ttctttatgt gaggacctgg gaagaagtca
     7621 gtcaggcact tcatcagcca aaggctgaac cacaaacaga gttagaagaa gcggaccaag
     7681 aactaaatct tttttcattt ctggaagagg agccagttca gagtattgga ctattggaac
     7741 ccgatggttc tgaaaaaggt caaaacgata ctgatcttga agaaacagat agtcaaattc
     7801 ctgaagaggt agtagtcgaa acaattccag agattccagt aacggacttt tattttccag
     7861 aagatatgac ggacttttat ccaaagactg ctagagataa ggttgagaca aacattgcgg
     7921 ccattcgttt ggtaaaaaat ctagaagtag agcaccgcaa tgcttcatca agtgaacaag
     7981 aactccttgc caagtatgtg ggctggggtg gacttgccaa tgatttcttt gatgactata
     8041 atccaaaatt ttctaaggaa cgagaagaac tgaaaagcct agtcacagat aaagagtatt
     8101 cggatatgaa acagtcctct ctgacagcct attatacagc ccctgccctg atccgtcaga
     8161 tgtgggataa gttggaaaga gatggcttta caggtggcaa aatcctagat ccttccatgg
     8221 gaacagggaa tttttttgcg gctatgccca aacacttaag agaaaagagt gagttatatg
     8281 gtgtggaatt agataccatt acaggagcta ttgccaaaca ccttcatcca aatagtcaca
     8341 ttgaaattaa gggctttgag acggtggctt ttaacgacaa tagttttgat ttggtgattt
     8401 caaatgtgcc ctttgccaat atacgaattg cggataacag gtacgatagg ccttacatga
     8461 ttcatgatta ctttgtcaaa aagtcacttg atttggtcca tgatggtgga caagtagcga
     8521 ttatctcttc aacaggaacc atggataagc gaacagaaaa catcttacaa gatattcgtg
     8581 agacaactga atttcttggt ggggttcgac tgcctgactc tgcctttaaa gccattgcgg
     8641 gaacgagtgt cacaacggat atgttattct tccagaaaca cttagacaag ggatatgtgg
     8701 cagacgactt agccttttca ggttccattc gttatgaaaa ggatagtcgc atttggctca
     8761 atccttattt tgatagggaa tacaatagcc aagtgctagg aacctacgag gtcagaaatt
     8821 ttaacggagg aacactttct gttaagggga ctagtgataa cttgattgca agtgttgaag
     8881 cagctctaca tcaagtaaaa gccccaagag agattgatag aaatgaggtc atcattaacc
     8941 cagatgtgtt gaccaaacaa gtcattgata cctccattcc agctgaaatg agggagaatc
     9001 taggtcagta cagttttggt taccagggta ctatgatcta ctatcgagat aataaaggca
     9061 ttcgagtcgg aaccaagacg gaagaaatca gttactatgt cgatgaagag ggtaacttca
     9121 aagcatggga caccaaacat tcccaaaaac agattgatag atttaaaagc ttagaagtta
     9181 cagatagcac agctctggat gtctatgtga ccgatgatgc agcaaaacgc ggacaattta
     9241 aggggtatta taaaaagaca gttttctatg aagctccttt gtctgataaa gaagtggcac
     9301 gaatcaaagg aatggtcgat attcgcaatg cttaccaaga ggtcattgcc attcaacgcc
     9361 attatgacta cgataagggg acctttaacc atttgttagg caaactcaat cgtacctatg
     9421 atagctttgt caaacgctat gggtatttga atagtgctgt gaaccgcaat ctttttgata
     9481 gtgatgataa gtattcgctt cttgctagtt tggaagatga aagtctggat ccaagtggaa
     9541 agtctgttat ctatactaaa tcccttgcct ttgagaaggc tctagtgcgt cctgaaaaag
     9601 aggttaaaaa ggtgcatact gcccttgatg ccttaaattc gagcttggct gacggacgag
     9661 gtgttgattt cgcttatatg atgtctatct atcaggttga atcgcagatg accttgattg
     9721 aggagttagg tgacctcatt atgcctgatc ctgagaagta tttgaatgga gaattgacct
     9781 atgtttctcg ccaagacttt ctgtcagggg atgtcgttac caagtcagaa gtggtggacc
     9841 tattcgtcaa acaagacaat caggacttta actggtcaca ttatgcagga cttctagaag
     9901 ctattaaacc agcacgtatt actttggcag acattgatta tcgaatcggt tcacgctgga
     9961 ttcctttgtc tgtttacgga aaatttgccc aagaaacctt tatggggaaa gcctatgaac
    10021 tgtcagacca agaagtagca acagtccttg aagtcagtcc cattgacggg gttatcactt
    10081 accaatctaa gtttgcctac acctattcca acgcaacgga taggagttta ggtgtccctg
    10141 cttcacgcta tgatagtggt cgaaaaatct tcgaaaatct cctgaattcc aatcaaccaa
    10201 cgatcacaaa acaagttgtc gaaggggata agaaaaagaa tgtgacagat gtggagaaaa
    10261 caacggtcct acgtgccaag gaaacacacc tacaagaact ctttcaaggt tttgtagcaa
    10321 agtatccaga agtccaacaa atgattgaag acacctataa taggctctac aatcgtacag
    10381 tatcaaagtc ctatgatggt agtcatttaa ccattgatgg acttgcccag aatatctcgt
    10441 tacgtcctca ccaaaagaat gccattcaac gaattgtaga ggaaaaacgt gctctactag
    10501 cccacgaggt agggtctgga aaaactttga ccatgcttgg agctggtttc aagttgaaag
    10561 aattagggat ggtgcataaa cctctctatg tggttccttc tagtttgact gcccagtttg
    10621 gtcaagaaat catgaaattt ttccctacca agaaagtcta tgtgactact aagaaagact
    10681 ttgccaaagc caaacgtaag cagtttgtgt ctcggattat tacaggggac tatgatacca
    10741 ttgtcattgg ggattcacaa tttgaaaaaa ttccaatgag tcgtgagaaa caggtcacct
    10801 atatcaatga caaacttgag caactcagag aaattaaact aggaagtgac agtgattaca
    10861 cggtgaagga agcagagcgt tcaattaagg gattagaaca ccaactagaa gaactccaaa
    10921 aactagagcg agataccttt atcgaatttg aaaaccttgg cattgatttt ctttttgtgg
    10981 atgaggctca tcacttcaag aatatccgtc caatcactgg acttgggaat gtagctggaa
    11041 tcaccaatac cacttcaaaa aagaacgtag atatggagat gaaggtgaga caggtacagg
    11101 cagagcatgg agatagaaat gtcgtttttg cgacaggaac accagtttct aactctatta
    11161 gtgaactttt caccatgatg aattacattc aacctgatgt tttagagcga taccaggtat
    11221 ccaattttga ttcatgggtt ggggcttttg ggaatattga aaactctatg gaactagccc
    11281 cgacaggaga taagtaccaa cccaagaaac ggttcaagaa atttgtcaac cttcctgaac
    11341 tcatgcgaat ctacaaggaa actgccgata ttcagacctc agatatgctc gacttacctg
    11401 tacctgaagc taagattatt gcggtggaaa gcgagttaac gcaagctcag aaatactatt
    11461 tagaagagct ggtagagcgt tcggacgcta tcaagtcagg tagtgttgat ccaagtagag
    11521 ataacatgct taaaatcaca ggagaagcca gaaaactagc cattgatatg cggttgattg
    11581 accttgctta ctccttatcg gataatcaga aaatccttca agtagtcgat aatgtcgagc
    11641 ggatttaccg tgaaggagct gaatacaaag ccactcagat gattttcact gatattggaa
    11701 cacctaaaag taaggaagaa ggctttgatg tctacaatga actgaaagct ttgctggttg
    11761 atcgagggat tccaaaagaa gaaattgcct ttgtccatga tgccaatact gatgagaaga
    11821 aaaactctct gtcacgtaag gtcaatagcg gagaagtacg gattctcatg gcttcgactg
    11881 aaaaaggtgg aacgggtcta aacgtgcaat cacgcatgaa agctgtccac cacttagatg
    11941 ttccgtggcg tccctcagac attgtccagc gaaatggacg actaattcga caaggaaaca
    12001 tgcaccagga ggtagatatt tatcactata ttactaaagg gagcttcgac aattacctct
    12061 ggcagacaca ggagaataag ctcaaataca ttacgcagat aatgacctca aaagatcctg
    12121 tgagatcagc tgaagacatt gatgaacaaa ccatgactgc ttctgacttt aaggctttag
    12181 ccactggtaa tccctacctt aagctcaaaa tggagttgga aaatgaactg acagttttag
    12241 aaaatcaaaa acgcgccttt aatcgctcca aagacgagta tcgccatgca atttcctatt
    12301 gcgagaagca cctccctatt atggaaaaac ggttgagtca atatgataaa gatattgccc
    12361 aatctttggc aacaaagtcg caagattttg tcatgcgatt tgacaatcag aaaatggata
    12421 atcctacaga agcaggggac tacctgcgaa aactcattac ctataaccgc tcagagacca
    12481 aggaagtcag aacacttgcc agctttagag gatttgactt aaaaatgact acacgaggtc
    12541 ctagtgagcc cttgccagag actgtctctt taatgattgt aggagataat cagtatactg
    12601 tcgcccttga tttgaaatca gacgtgggaa ccattcaacg aattagtaat gccattgacc
    12661 atattttgga tgaccaagaa aagacgcaag agctggtaaa ggatttaaaa gataagctac
    12721 aagtagccaa agttgaagtt gagaaaatct ttcccaagga agaggaatat cagcttgtaa
    12781 aggctaagta tgatgtcttg gctcccttgg ttgaaaaaga agcagagatt gaagagatag
    12841 atgcagcttt ggccaagttt agtgaagata caacagccca aaagaagcaa caactagcac
    12901 tcgagatata agaaaaaagg ctgacaaata tttttgaacg cggtaaaatg aagtcaagaa
    12961 atcacaaaag gagaacagac catgaccaaa acagtagaag aaatgcgtta tcaacttgaa
    13021 gaatggttgt cacaaggctt tacgagttca gaagataggg caaactacca aactttaaag
    13081 gaacagtatg aagatgaaac tcttgattat agcttttcaa agcgtgaaat tactggacag
    13141 ctggaactca tcatcacaac tcgtgagaat gattttccaa gcttagatga ggtaacgaag
    13201 gcagaatacc ttgatttggt tgcccaactt gatgatttag acaagagaca ggctgactac
    13261 tatcgcaagc aattagccta gaaagaggtg taagatgttg gagcaaattc tacaaagcct
    13321 tttgattatc gcagcaatag gactgatgtt gttagtcctt tatcggattg tgaaagtttc
    13381 aggtgcttta tttcttatcg gactcatcag tggattagtc tttattgaaa tctatggagt
    13441 ttacctcttc tttactgaga gatacctcta ttcagaagat ttagcaacta atggagtttg
    13501 gagttttaca ggatttttta ttgctttgaa tctatttctc gttttaagct ttattataaa
    13561 gtggtggaga aatagaatag tttaacacgg aggattaccg attggtagtc ctttttcttg
    13621 tgataaattt tccaagataa tcttgttatg atttggctat cactaataaa ggagtaagcc
    13681 atgttaaata aagtgaaagc tcgatttctg attggagtag gaggtttaat cgcagtcagt
    13741 tttatggtca tgattggcta tacgattggc tcacaatccg tttcaaaaca aactgagcac
    13801 caaatacgag cagaagccaa taaacttgtg acaaaggaaa agcaggagga aagagcgaca
    13861 gtcctatcag atgagcttgt caaagaattt ctcacacaat atttcaccaa ggtccaacta
    13921 ggagaaaata acactcgtat caagccctac atgacggact cggcattttc agaagaggaa
    13981 acaaatcaga ataaagcgat taatcaagtt tataaagatt atatgcttga ctaccgattt
    14041 gaatcagcca gtatatttgt caatacagaa agcaatgttg cacttgcgga agtttcctat
    14101 caagtgacct atgtttctga tttgagtgag caacaacagc gaactactca gacagaaacc
    14161 aagacggtta tgttatccta ctccaaagtt tccgacaagc tcctggttaa tcagttgacc
    14221 atttggaatg ggaaactgga ggacatgaaa gaagcaacag atggagccaa ttccagcata
    14281 ccaacgatcc aaggaactac aacaagtgag aacaactagc aggagacatt cttctgcttt
    14341 ttttataggg agacaagatg acacgaatta aagcagtaaa acaaaaggcc attttaaatg
    14401 tggctgaaag tctgggttat tccttcagac gtttatcagg acacatttat gaacacccag
    14461 accatgattc ctttcggatt tttgcggata ccaatacttt caaatggttt tcaagagata
    14521 tacaagggga tgtgatagac tttgttcaat tagtggcagg cgttactttc aaagaggctg
    14581 tatcctatct tgaaactggg gattttgaac aagctaagtt gatagaagaa acttatcaac
    14641 cgtttcaata ttatttgcat gaagaaccct ttcagcaagc acgtatttac ttaaaagaca
    14701 tccgtggcct aagtgatcag actatcaata cctttggtag acaaggattg cttgctcaag
    14761 ctacttatca atcggagccc gtattagtgt taaaaagcta tgaccacaac gggaccttac
    14821 aggccgcaag ccttcaaggt ctcgtcaaaa atgaagaaaa acacgatcga ggttatctca
    14881 aaaaaatcat gaaaggatct catggctatg tcggtattag tttcgatatt gggaatccta
    14941 agcgactcat tttttgtgaa tcagttatcg atatgatgag ttattatcag atttaccaaa
    15001 agcaattatc cgatgttcgc ctgatttcaa tggaaggttt aaaactttct gtgattgctt
    15061 atcagacctt gcgtctagca gcagaggaac aggggaaatt ggcatttcta gatacagtaa
    15121 atccaagcag acttagtcat tatcttcagg caatacaaga gacgacaacc ttcttccaaa
    15181 ctcattcaaa tgcattaaca ttggctgttg ataacgatga ggcaggaaga gaattttgtc
    15241 agaaactgtc agataaagga cttccgcttt ctcaagattt accaccattg caaggacttg
    15301 aaacaaagtt agattggaat gatattgtga aacgacaaaa ggaattatct ttaagagatc
    15361 ttatccagtc agcccaaaca aaagtcatta gagatcatcc tccacctaaa cgagagcaca
    15421 cttttgaatt gtgataacaa aatcaagtcc gctatcataa tctagaatgt gacaaggagg
    15481 acaccctatg agtgtgattg aacgtctggc tgaaaaagta gctagacaag aagaaaaggt
    15541 ctcacgtgag acggagaaat tggaacacta tcgtgaccaa ctacaaacag ctatgtacag
    15601 tacctttatc aaacggcaac aatctagtca gttgtcattt catgaagcac tagagcaagc
    15661 ctttggtaaa gaaaccacac tacacccaaa ttacagaaat gaggatacag aatgagtaaa
    15721 acatggaatt ttgatcagcc actagatgat gtgaaaccaa catcatccca tgaagaacga
    15781 gcaaaaattg cagcactttt ccataaacaa gatgaaaaac caattgaaga agtagactat
    15841 gtggctgctt ttgaacagca acaaaaggag tcagaatcta aagatgtaca gacgcccgaa
    15901 tcaaaagtta aacaaagcca gccgaagaaa gtaaatatca caagcgacta taaacaacac
    15961 ttggcagaca ctattgcaca aaacaacaag gatatttcag cctgtcaaaa gcaaattgaa
    16021 gaacttcatc aattgattga tgaaaagaaa atccaaaata aaaagttgca ggctatttca
    16081 gtagccattg atgatttata agtcaggtag tcctatgcgg gctacctttt tataattgaa
    16141 ggagaagata atgaaatttt ttcaaaagaa aaaacagaac caggatcaat ttaaacgact
    16201 gattcatcga ctatctgaga tgtcagatac tgaactaacc aaagtagaac aactcctaga
    16261 tgtggttttt gatacaaatt ttaagcctga tagaaacgtt gaattatctc aaagcgttat
    16321 aaccgatgag gaacaaagtc ttgacaaatc gatccaagaa gcgaaaaata aactgaatac
    16381 tgagcagttg gaaaaaaaca ttgagagatt tagacaaggt aggcgagaga gcaataataa
    16441 caaataacag accaattatt ttggcctgtt ttttgaaata tgtgtcatcg ttgaatcaaa
    16501 cgaagtaaaa atatttcttg cttagtagga ggtgttgtgg tataatttag ttagattaga
    16561 agtcatcgaa ggtacagcaa tgtataacta ggtggccttt tttcatattg cggagagata
    16621 ttttatgcct aataatgtat ttgaattaat aaattttaag gactattttc cgttagattt
    16681 agatattact gataaaacta ggttggttta tagaccagta ttagcaccaa ctc
//
LOCUS       DBNFEG010000030        16703 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.030, whole genome shotgun sequence.
ACCESSION   DBNFEG010000030 DBNFEG010000000
VERSION     DBNFEG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16703
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.030"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1200
                     /locus_tag="AC1PNG_000669"
     CDS             <1..1200
                     /locus_tag="AC1PNG_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIP6701190.1"
                     /translation="CAFASFLVACGNASSSDKVEIEYFSQKPEMQATLQEIIDDFEKE
                     NPTIDVKFSNVPDAGTVLKTRMANNEAPDVINIYPQNADFKAYAADGRFLEIGDDAGL
                     SHLKDGAVTPYLVNEKNYTLPLTANAYGIYYNKDKFKELGLEVPTTYAEFVALVDKIK
                     ADGSAAPFALSLNDAWSLNGYHQLAWVTVAGGFDGAEDILIRSAKGAIQDDATTKAVL
                     ERLQLLKDNGQKGATGALYADAVAAFAAGDALMLPQGTWAATAVNQQEPEFEYGMFTF
                     PGDKEGGDYTIGAADLALSISADTEHPEESKKFLEYLSRPEVIQKYYDVDGSSTSVEG
                     VDTEGKFEETAGVTQYAFTDKHVVWLQSEWESEDEFWNITVEMVKNPNSAELVKKLNA
                     FFDPMKK"
     gene            1337..2209
                     /locus_tag="AC1PNG_000670"
     CDS             1337..2209
                     /locus_tag="AC1PNG_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIP6701191.1"
                     /translation="MNQKGFIAKYWPYLFVAIPISLQLIFFFYPLFTGIFYSLTDWNG
                     LTSDFSLIGVQNYLDILKNPDFYTSMTFTIIFTIGLVIGEIVIGIWLASLLNRKIKAV
                     GFFRAWYFFPAVLSTVTLGLIFVQLFNYGFTQIGEILHIDWLMENLLVQENTVIPAVL
                     FVALWQGLAMPVIIFLSGLQSIPEDVKEAAAIDGATRSQQFFNIELPFLLPSISMVFI
                     LAMKSGLTAFDLIFALTSGGPDGKTESLGLLVYNYAFVDNKFSYANALAVVLFIFIIV
                     ISLIQMRISKKFEI"
     gene            2220..3050
                     /locus_tag="AC1PNG_000671"
     CDS             2220..3050
                     /locus_tag="AC1PNG_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIP6701192.1"
                     /translation="MNTQKQKNWWVYLVLFSGILFMFIPLLVTIISSFKPTKEITSNF
                     FGLPENFTLNNYERLFNDGIVQYFGNSALITIVAVGLILLVIPMAAFAVARQMKRQTV
                     FNFIYFFLIIGIFVPFQVIMLPMTKLMSSLGLNNIVGLIILYLTYAVPQALFLYVGYI
                     KTIVPEEMDEAAAIDGCDKFTMYWKIIFPLMKPMHATVLIINALWVWNDFLLPLLVLN
                     RDQSMWTLPLFQYNYQGMYFSDYGPSFASYVVGIIPILLVYLVFQKHIISGMTSGSVK
                     "
     gene            3119..4567
                     /gene="gtfA"
                     /locus_tag="AC1PNG_000672"
     CDS             3119..4567
                     /gene="gtfA"
                     /locus_tag="AC1PNG_000672"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HIP6701193.1"
                     /translation="MKIKNQAMLITYSDSLGKNLKDLKKVLEGPLKDVVGGIHILPFF
                     PSSGDRGFAPMDYTKVDPAFGDWSDIEALSKDYYLMFDFMINHISAKSPYFLDFLEKK
                     DESAYADLFIRYKNFWPNGEPTQEDVDLIYKRKPRAPYRIATFADGSEEKVWCTFDEQ
                     QIDLDVTTETTRRFIQENLEQLAEHGASIIRLDAFAYANKKLGTNCFFVEPDIWEMLH
                     FPRQLLAPKDVEILPEIHEHYTIQQKIAEQDYYVYDFALPMLVLHALYSGHVNRLVHW
                     MEICPRKQFTTLDTHDGIGVVDVKDLLTDEETEETREALYAQGANVKKIYSTEAYNNL
                     DIYQINCTYYSALGNNDQAYLLARVLQCFAPGIPQIYYVGLLAGENDIELLESSKEGR
                     NINRHYYDLEEIEQEVQRPVVQSLFKLLKFRNTSPAFDGEFSVKMLDETSMEIFWNNQ
                     DAGVSARLTANLKEKSFEIVEIEEGKITTIEL"
     gene            4640..6256
                     /locus_tag="AC1PNG_000673"
     CDS             4640..6256
                     /locus_tag="AC1PNG_000673"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_function: GO:0004574 - oligo-1,6-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009311 - oligosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIP6701194.1"
                     /translation="MSEIQKTDWWKKSVIYQIYPRSFQDSNGDGIGDIRGIISRLDYL
                     HELGIDAIWLSPVYQSPMDDNGYDISDYQGIAPEFGTMEDMEELIAEGHKRNIKIIMD
                     LVLNHTSDEHFWFQEALKGPDNPYYDYYVWADEPNELRSTFSGSAWEYVPHLNKYYLH
                     QFSVKQPDLNWKNPALKQEIWDMINFWIEKGVGGFRLDVIDLIGKEPEKLITADGPTL
                     HPLIQELNEKTFGAYDLVTVGETWSANPENAQLYSHPDRKEFSMIFQFEHVGLDQQEG
                     KEKWDLAPLDPARLHKVLSKWQTELVGKGWNSLFWNNHDLPRAISRFGDDRPAFRELS
                     GKMLAIYLHFMSGTPYIYQGEEIGMINTPITDISQADDIETRRMYAERIENGFTKEEL
                     ITSINAKGRDNARRPMQWTAEEGAGFSTGQAWLALGDTVKDINVEKALADKQSLFYTY
                     QKLIALRKEYPCMSEGKYEVMETGNDLVMAYRKYDEQDDFIILVNFTSEEQAYEFSLE
                     DYSVFMHNYDNKVIFDKSVLRPYEAIVFKK"
     gene            6339..6968
                     /gene="upp"
                     /locus_tag="AC1PNG_000674"
     CDS             6339..6968
                     /gene="upp"
                     /locus_tag="AC1PNG_000674"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIP6701195.1"
                     /translation="MGKFQVISHPLIQHKLSILRRTSTSTKDFRELVNEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQIAGKKLAIVPILRAGVGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRHIFVVDPMLATGGSAILAVDSLKKRGASNIKFVAL
                     VSAPEGVKALQEAHPDIDIYTAALDEKLNEKGYIVPGLGDAGDRLFGTK"
     gene            7076..7675
                     /locus_tag="AC1PNG_000675"
                     /pseudo
     CDS             7076..7675
                     /locus_tag="AC1PNG_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
     gene            7802..8074
                     /locus_tag="AC1PNG_000676"
     CDS             7802..8074
                     /locus_tag="AC1PNG_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HIP6701196.1"
                     /translation="MFEKKNRTCLTVYLHYNRDARKLSQYGDIVYHSKRLRYVLVYMD
                     QELVEATILKLKKERFVKKVVPSYIKELDQNFVGNLWRDEEPSVVG"
     gene            8205..9374
                     /locus_tag="AC1PNG_000677"
     CDS             8205..9374
                     /locus_tag="AC1PNG_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP6701197.1"
                     /translation="MKTRKFAVALATFASAALLAACGNVSTTNTSAAGSSVGDTFKIG
                     YNLELSGAVSSYGQTEEKGANLAVKEINAAGGIDGKKIEVITKDNKSETAEAATVATS
                     LASEGANVVIGPATSGASAASIPALTSAGVPMITPSGTQTNLVVNDKGEVQEYFFRTT
                     FTDGYQGQIMAKYATENLSAKKVVLYFDNSSDYGKGVAEAFKKAYTGEIVSEITFASG
                     DKDFQAALTKLKDKEFDAIIMPGYYNETGTIVKQARGLGIDKPILGSDGFDSPQFTEL
                     ATASAASNVYYLSGFVTSASEKAKAFYDAYVKEYNEEPSMFSALAYDSVYMAAEAAKG
                     AADSAAVKANLAALKDFKGVTGTMSVDKEHNVIKSVYVVSLTNGEESSVDTISID"
     gene            9580..10467
                     /locus_tag="AC1PNG_000678"
     CDS             9580..10467
                     /locus_tag="AC1PNG_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIP6701198.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDLYMMGA
                     FMGYFLLNNLNFIADGGTRFFVALILAMVGTAILGVVIEFLAYRPLRNSTRIAALITA
                     IGVSFFLEYTMIKFFTADVKAFPQAIEAVTFNLGPVSVTNIQLIILGVSLALMVALQL
                     IVKRTKMGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLLGLYYNQIE
                     PLMGMTPGLKAFVAAVLGGIGIIPGAALGGFVIGIIETFTYVIEFDTFRDAIVYTVLI
                     IILLVRPSGILGKNVKEKV"
     gene            10470..11417
                     /locus_tag="AC1PNG_000679"
     CDS             10470..11417
                     /locus_tag="AC1PNG_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIP6701199.1"
                     /translation="MKQNLKVNAIWLAILLAGFGLIQGLVSAGILNFYYIQIVQQIGI
                     QIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAVGILGKMMPTYGGFAIALIVGMLISG
                     AVALLVGIPTLRLKGDYLAIATLGVAEIIRILIINGGSVTNGAAGIMSIPLFTSWPLV
                     YIFVIITTLVTVNFLRSPMGRATISVREDEIAAESVGVNPTFVKVSAFVFGAMTAAIA
                     GGLHAGYVGTIVPKDFAFMTSVNVLIIIVLGGLGSITGTFVAAIVLGILNVFLKSYSD
                     ISMIIYSLALILLMIFRPGGLLGTKEFSVAALLKKKEVK"
     gene            11417..12181
                     /locus_tag="AC1PNG_000680"
     CDS             11417..12181
                     /locus_tag="AC1PNG_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6701200.1"
                     /translation="MALLEVKDLTKNFGGLTAVGDVTMELHEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTISLAGTILNGKAPSKIASLGLGRTFQNIRLFKNMTVLENVLIG
                     LGNHGKSEVFASFFRLPAFYKNEEALKTKAIELLKIFDLDGDADTLAKNLPYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTQLIRKIKEEFGITIILIEHDMSLVMEV
                     TERIYVLEYGRLIAHGTPEEIRNNKRVIEAYLGGEA"
     gene            12181..12891
                     /locus_tag="AC1PNG_000681"
     CDS             12181..12891
                     /locus_tag="AC1PNG_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6701201.1"
                     /translation="MAMLEVKNLSVNYGVIEAVKDVSFEVNEGEVVTLIGANGAGKTS
                     ILRTISGLVRPSAGTISFLGNEIQKVPARKIVADGLSQVPEGRHVFAGLTVMENLEMG
                     AFLRNNREENQANLKKIFARFPRLEERKNQDAATLSGGEQQMLAMGRALMSQPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAIADRGYVLETGKVVLSG
                     TGKELLASEEVKKAYLGG"
     gene            13262..14017
                     /locus_tag="AC1PNG_000682"
     CDS             13262..14017
                     /locus_tag="AC1PNG_000682"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HIP6701202.1"
                     /translation="MIKVEEILSTLSPNQKVNYDKVFQKMASTWQEAGLRPKILMHVC
                     CAPCSTYTLEYLTQYADVTIYFANSNIHPKAEYQRRAAVTAQFVKEFNQKTGQSVGYL
                     EADYSPQEFFKKTQHLANEPEGGDRCKVCYDYRLDKTAEKAVELGFDYFGSALTISPH
                     KNSQTINAAGIEVQKLYGTQYLPSDFKKNGGYQRSVQMCEEYDIYRQCYCGCVYGANA
                     QGVNLVQVKKEALEFLKTHRDYSHIPFKVLDKN"
     gene            14125..14715
                     /locus_tag="AC1PNG_000683"
     CDS             14125..14715
                     /locus_tag="AC1PNG_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701203.1"
                     /translation="MKLRKVSFILVTFVIGLLSLSSVKAETSMFVPVGQQNVPGVEVA
                     PFRTGSDSIHVHVGSFGYVATYINGERLKVEPVKHELKCPSYTTENCQTKEWYTSGER
                     ADGSGDYVVKLNKKLEEGDVVTLKFADDGNLYFGQLVYKSEKKRVEQTQKEQEDEYAD
                     ALFKRSIEEEENKTWRDRIKDTFQDAWWNFKGWWNS"
     gene            14865..15896
                     /gene="holA"
                     /locus_tag="AC1PNG_000684"
     CDS             14865..15896
                     /gene="holA"
                     /locus_tag="AC1PNG_000684"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIP6701204.1"
                     /translation="MTVIEQIEKLKKEKLELLTVLCGEDIGQYQIAKDLLLRKLDFDA
                     ADLGFAYFDMSEADYHQVDLDLVSLPFFSDEKVVILDYFADLTTDKKRHLTDDELKQF
                     EVYLENPVETTCLVILAPGKLDSKRRLVKLLKRDGLVLEANPLKEMDLKNHFQKEIMR
                     LGLQMGGEVFQYLLEKSNFDFAEISKNLVFLQSYKGKETIEISDIDAAIPKTLQDNIF
                     DLTQLILQSKVDEARNLVRDLRLQGEEEVKLIAIMLTQFRTYLQVQILLEQGRGEQQI
                     VAELSTIMGRKVNPYQVKYALRDSRHLSIGFLKKVVKLLIDTDFQIKTGQFDKDYLFD
                     LLLLKIATA"
     gene            16009..16614
                     /gene="sodA"
                     /locus_tag="AC1PNG_000685"
     CDS             16009..16614
                     /gene="sodA"
                     /locus_tag="AC1PNG_000685"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase SodA"
                     /protein_id="HIP6701205.1"
                     /translation="MAIILPDLPYAYDALEPHIDAETMTLHHDKHHATYVANANAALE
                     KHPEIGEDLVALLSDVEQIPADIRQALINNGGGHLNHALFWELLSPEKTEISAELAAD
                     IDATFGSFDAFKEAFTAAATTRFGSGWAFLVVNKEGKLEVISTANQDTPIMQGLKPIL
                     ALDVWEHAYYLNYRNVRPNYIKAFFEVINWDKVNELYKAAK"
BASE COUNT         4989 a         2984 c         3616 g         5114 t
ORIGIN      
        1 tgtgcctttg ccagcttctt ggttgcttgt gggaatgcaa gtagtagcga taaggtagaa
       61 atagaatatt tctcacaaaa acctgaaatg caggctactc ttcaggaaat tattgatgat
      121 ttcgaaaaag aaaatcctac gattgatgtt aaattttcaa atgtaccaga tgcaggaact
      181 gttctgaaaa cccgtatggc aaataacgaa gcgccagatg tcatcaacat ttatccacaa
      241 aatgcggact tcaaagcata cgcagcggat ggtcgtttcc tagaaattgg agatgatgca
      301 ggtctcagtc atctaaaaga tggtgctgta acgccatatc ttgttaatga aaaaaattac
      361 acccttcctt tgacagcaaa tgcctatggt atttactata ataaggataa gttcaaagaa
      421 ttggggctcg aagttccaac tacctatgca gaatttgtgg ctttggtaga caaaatcaag
      481 gctgatggca gtgcagcacc ttttgccctt tcattgaacg atgcttggtc attgaacggc
      541 taccatcagt tagcttgggt aactgttgcg ggtggatttg atggtgcaga agatattctg
      601 attcgtagtg ccaaaggcgc aattcaagat gatgcgacaa caaaagcagt tttagaacgc
      661 ttgcaattgt tgaaagacaa tggacaaaaa ggggcaacag gcgcgctcta tgcagatgcg
      721 gtggcagcct ttgcagcggg tgatgctctc atgcttccac aaggtacatg ggcagctaca
      781 gctgtaaacc aacaagaacc agaatttgaa tatggtatgt ttacattccc tggcgataaa
      841 gaaggtggcg actataccat cggtgcagct gaccttgctc tatctatttc agcagataca
      901 gagcacccag aagagtctaa aaaattccta gaataccttt ctcgtccaga agttatccag
      961 aaatactatg atgtagatgg ctcgtcaact tcagttgaag gtgtagatac agaaggtaag
     1021 tttgaagaaa ctgctggagt tacacaatat gccttcactg ataaacacgt agtttggttg
     1081 caatctgaat gggaatcaga agatgagttc tggaacatta ctgttgaaat ggttaaaaat
     1141 ccaaactcag cagaattagt gaaaaaactg aacgcattct ttgacccaat gaaaaaataa
     1201 ttcaagaaac tataaggagg gctagaacag cctagctctt ctttatagtt gcttagggtc
     1261 agtttgaata ccccctaacc actccattca aactgaccct aagtaactat acattttaga
     1321 aaaaaggagt accatgatga atcaaaaagg ttttattgcc aaatattggc cctatctatt
     1381 tgttgccata ccaatcagtc tacagttaat ctttttcttc tatcccctgt ttacaggtat
     1441 attttacagt ttgacagact ggaatggatt gacatctgat ttcagcttga ttggcgttca
     1501 aaactatcta gatatcttga agaatcctga tttttatacg tctatgactt ttaccattat
     1561 cttcaccatt ggtttggtca tcggagaaat tgttataggg atttggttgg ctagtctcct
     1621 caatcgtaag attaaagctg ttggtttctt tagagcctgg tatttcttcc ctgcggttct
     1681 ttcaaccgtt actttaggct tgatttttgt tcaattgttc aactatggtt ttacacagat
     1741 tggagaaatc cttcatatcg attggttgat ggaaaactta ttggtacaag aaaatactgt
     1801 aattccagcc gtactttttg tggctctctg gcaaggattg gctatgccag tcatcatctt
     1861 cctgtctggt ttacaaagta ttccagaaga tgtgaaagaa gcagcagcga ttgatggtgc
     1921 tactcgttct caacaattct tcaatattga acttccattc ttattaccat ctatcagtat
     1981 ggttttcatc ttggcaatga aatcaggttt gacagcattt gaccttatct ttgcattgac
     2041 aagtggtggc ccagacggca aaacagagtc cttgggttta ctcgtttaca actatgcctt
     2101 tgtagacaat aaattctctt atgccaatgc tttagctgta gtactcttca tcttcattat
     2161 tgtcatctca ttgattcaga tgagaatttc gaagaaattt gaaatttagg aggacagaca
     2221 tgaatactca aaaacagaaa aattggtggg tctatcttgt gctcttcagc ggtattctct
     2281 tcatgtttat tccgctactt gtaacgatta ttagctcatt caaaccaacc aaggaaatca
     2341 cgagcaactt ctttggtctt cctgaaaatt tcaccttaaa taactacgaa cgcttgttta
     2401 atgatgggat tgtacaatat tttggcaatt cagccttgat tacgattgta gcagttggct
     2461 tgattctact tgtaattcca atggcagctt tcgccgtagc ccgtcaaatg aaacgccaga
     2521 cagtgtttaa ctttatctac tttttcttga ttattgggat ttttgtacct ttccaagtga
     2581 ttatgctccc aatgaccaaa ttaatgtcaa gccttggttt gaacaatatt gtcggtttga
     2641 ttattctata tttgacctat gcagttcctc aggccctctt cctctatgtc ggctatatta
     2701 agactattgt tcctgaggaa atggatgagg cggcagctat tgatggctgt gataaattta
     2761 ccatgtactg gaaaatcatt ttcccactca tgaaacctat gcacgcaacc gttttgatta
     2821 tcaatgccct ctgggtctgg aatgattttc tcttaccgct cttggttttg aatcgtgatc
     2881 aaagcatgtg gacgttacct cttttccaat acaattacca aggcatgtat tttagtgact
     2941 atggaccatc atttgcttcc tacgttgtag gaattatccc aatcttattg gtataccttg
     3001 tcttccagaa acatatcatt tcaggtatga caagtggttc tgtcaaataa ttacaaaggt
     3061 tgacttgttc aaccttttct aaaaataaaa aagaaaaggc ttacaaagga gaatatcgat
     3121 gaaaattaaa aatcaagcta tgttgattac ttactcagac agtttgggta agaatctcaa
     3181 ggatttgaaa aaagttcttg agggaccatt aaaggatgtt gtgggaggta ttcacattct
     3241 accattcttc ccatcatcag gtgaccgtgg gtttgcacca atggattata caaaggtaga
     3301 tcctgcattt ggagattggt cagatattga agcattgagt aaggactact atctgatgtt
     3361 cgattttatg attaatcata tttcagccaa gtcaccgtat ttccttgatt ttcttgaaaa
     3421 gaaagatgaa tcagcttatg cggatttgtt tattcgttat aaaaactttt ggccaaatgg
     3481 tgagccaacg caagaagatg ttgatttgat ctataagcgt aaaccccgtg ctccatatcg
     3541 aattgcaaca tttgctgatg gtagtgaaga gaaggtgtgg tgtacctttg atgagcaaca
     3601 gattgactta gatgtgacaa cggagacaac ccgtcgtttc atccaagaaa atctggaaca
     3661 attggcagaa catggtgctt cgatcattcg tttggatgcc tttgcttatg ccaataaaaa
     3721 actcggaacc aattgtttct ttgtggagcc tgatatctgg gaaatgctac attttccacg
     3781 tcagttattg gcgccaaaag atgtggaaat cttaccagaa attcatgaac actataccat
     3841 tcagcagaaa attgccgagc aagattatta tgtttatgat tttgccttgc caatgctagt
     3901 tctccacgct ttgtattctg gtcatgtcaa tcgcctggtt cactggatgg agatttgccc
     3961 tcgcaagcaa tttacaactc ttgatacaca tgatggaata ggcgttgtgg acgttaagga
     4021 tttgctgacg gatgaggaga cagaggaaac gcgtgaagct ttgtatgcac aaggtgccaa
     4081 tgtgaagaaa atttatagta cagaagccta taataatttg gatatttatc agattaactg
     4141 tacttattat tctgcccttg gcaataacga tcaagcttac ctattagcgc gtgttctcca
     4201 atgttttgca ccaggtattc cacagattta ctatgttggt ttacttgcag gtgaaaatga
     4261 tattgaactt ttagaatcaa gtaaagaagg acgcaatatc aatcgccatt actatgattt
     4321 ggaagaaatt gagcaggaag tgcaacgtcc tgtggtacaa tccttgttca aactccttaa
     4381 attccgcaat acaagtccag ctttcgacgg agagttctct gttaagatgc tggatgaaac
     4441 aagtatggaa attttctgga ataatcaaga tgcaggggtg tctgcccgtc taacagcaaa
     4501 cctaaaagaa aaatcctttg aaattgttga aatagaagag ggcaaaatta ccacaattga
     4561 gttataatat gattatatag aaataggggg attcctcttt ttcttgcgta aataagattg
     4621 tacagaaagg atcaatcaaa tgtcagaaat acaaaagaca gattggtgga agaagtccgt
     4681 catttaccaa atttatcctc gtagttttca agattcaaat ggagatggaa ttggagatat
     4741 tcgaggaatt atcagccgat tagattatct ccatgagctt ggaattgatg ctatttggct
     4801 cagtcctgtt tatcagtccc ctatggatga caatggttac gacatcagtg actaccaagg
     4861 aattgctcca gaatttggga ccatggaaga tatggaagag ctgattgcag aaggccataa
     4921 gcgcaatatc aaaataatca tggatttagt gctcaatcat acctcggatg aacatttctg
     4981 gtttcaagaa gctctcaaag gtccagataa tccctattat gattattatg tttgggctga
     5041 tgagccgaat gaattacgct cgaccttctc aggttctgct tgggaatacg ttccacatct
     5101 gaacaaatac tatctccacc aattctctgt taagcaaccg gatttgaact ggaaaaatcc
     5161 tgctctgaaa caagaaatct gggatatgat caatttttgg attgaaaaag gtgttggcgg
     5221 tttcaggcta gatgtaattg atttgattgg aaaagaaccg gaaaaattaa ttacagccga
     5281 tggaccgact cttcatcctc ttattcagga attaaatgag aaaacctttg gtgcttacga
     5341 cttagtgact gttggggaaa cctggagtgc caatccagaa aatgctcagt tatattcaca
     5401 tcctgaccga aaagaatttt cgatgatttt ccaatttgaa catgtcggac ttgatcagca
     5461 ggaagggaaa gaaaagtggg atttggcacc gcttgatcca gcacgtttgc ataaggttct
     5521 atccaaatgg cagactgaac tggttggtaa aggctggaac tcgcttttct ggaataacca
     5581 cgatttacct cgtgccattt ctcgcttcgg tgatgatcga cctgcatttc gtgaactaag
     5641 tggtaaaatg ttagctattt atcttcattt tatgtcagga acaccttata tctaccaagg
     5701 tgaggaaatc ggtatgatca atactccgat tacggatatt agccaagcgg acgatattga
     5761 aacacgtcgc atgtatgcgg aacggattga aaatggcttc accaaggagg aattgattac
     5821 ttctataaat gccaaaggtc gtgataatgc tcgccgtcca atgcaatgga cagcagaaga
     5881 aggtgcaggt ttcagtacag gtcaagcctg gctggctttg ggagatactg tcaaagacat
     5941 caacgttgaa aaagcacttg cagacaaaca atcgcttttc tatacctatc aaaaattaat
     6001 tgctcttcgg aaagagtatc cttgtatgtc tgaaggaaaa tatgaagtga tggagacagg
     6061 gaatgacttg gtaatggcct accgtaaata tgatgaacag gatgatttca ttattttagt
     6121 caatttcaca agcgaagagc aagcgtacga gttttcactt gaggactatt ccgtgtttat
     6181 gcataattac gataataaag taatttttga taagtctgta ttgagacctt acgaagctat
     6241 cgttttcaaa aaataagtgg tacttcattt cttttcccat gcttttttca tgaatttttg
     6301 gtaaaataga aaaaacaaaa aagagaggtt ttatcatcat gggtaaattc caagtcattt
     6361 cacacccact cattcagcat aagttgtcta tccttcgtcg tacatctact tcaacaaagg
     6421 attttcgtga gttggttaat gaaatcgcga tgctgatggg ttacgaagtt ttgcgtgact
     6481 tgccacttga agatgtggag attgaaacac caatcactaa aacagttcaa aaacaaattg
     6541 caggtaaaaa attagctata gtaccaattc ttcgtgcagg tgttggtatg gttgatggtt
     6601 tgctaagctt ggtcccagca gctaaagttg gtcacatcgg tatgtaccgc gatgaagaaa
     6661 cccttcaacc agttgagtat cttgtgaaat taccagaaga tattgatcaa cgtcatattt
     6721 ttgtagttga cccaatgctt gctacaggtg gttcagccat ccttgcagtg gattcattga
     6781 aaaaacgtgg tgcttcaaat atcaaatttg ttgctcttgt ttcagctcca gaaggtgtaa
     6841 aagctcttca agaagcacat ccagatattg atatctatac ggcagccttg gatgaaaaac
     6901 tcaatgaaaa aggctacatc gtaccaggtt tgggagatgc aggtgaccgc ttgttcggta
     6961 caaaataatt aagaaatcag ctctgctggt ttctttttca tttcctgaaa tcgtgttttt
     7021 tatttgacca attttgacta ttcatatata atgggtacag aaaacctcga aaaggagtaa
     7081 ttttatgatt ccagtagtta ttgaacaaac tagccgtggt gagcgttctt atgatattta
     7141 ctcacgcctt ttgaaagacc gcattatcat gttgacagga ccagttgagg acaacatggc
     7201 aaactctatc attgcacaat tgcttttcct tgatgcccaa gaccctacaa aggatattta
     7261 cctctatgtt aatacgccag gaggatcggt gtcagcaggt cttgccattg tagacacgat
     7321 gaatttcatt aaagctgatg ttcaaaccat cgttatggga acagctgcga gcatgggaac
     7381 catcattgca tcaagcggtg ccaagggcaa acgtttcatg ttgccaaatg cagagtacat
     7441 gattcaccag ccaatgggtg gaactggtgg tggtactcag caaacagata tggctattgc
     7501 tgcagaacac ctattaaaaa cacgtaataa gctagaaaaa atcttggcag acaactcagg
     7561 taagacagtc aagcaaatcc acaaggatgc agaacgtgat tactggatgt cagctgaaga
     7621 aaccttggct tatggattta ttgaccagat tatggacaat acaaaagtca aataagcagt
     7681 gacaaatatg taatagtact gactattcct attagctcca actcttttac aggtagttgg
     7741 agcttttcat ttatcattga aaacggtata atatagaaag agaattttta gaggagaaga
     7801 catgtttgag aagaaaaatc gcacttgctt aactgtctat ttacactaca atcgtgatgc
     7861 acgtaaactc agccaatatg gagatattgt ttaccattcc aaacgcttgc gatatgtttt
     7921 ggtgtatatg gatcaagaac tagtagaagc taccatatta aaattgaaaa aggaacgttt
     7981 tgtaaaaaaa gtggttcctt catacattaa ggaattagac caaaactttg taggtaatct
     8041 atggagagat gaagaaccat cagtcgtagg atagtcggaa ttagcaggta tgtattccct
     8101 gctaattttt tattttaaaa tttttttctt ttttgttgac aattttcaga taattcagta
     8161 tattagatac atcgtaaaat ttaagaaaga tttaaggagt tttcatgaaa acaagaaaat
     8221 ttgcagtagc gcttgctaca tttgcctctg cagctctact cgctgcatgt ggtaatgttt
     8281 cgacaaccaa tacttcagcc gcaggttctt ctgtaggtga tacgtttaaa attggctaca
     8341 acttggagtt gtcaggtgct gtttcatctt atggtcaaac agaggaaaaa ggtgccaacc
     8401 ttgccgtgaa agaaatcaat gctgcgggtg gtattgacgg caaaaaaatc gaagtgatca
     8461 caaaagacaa caagtctgaa acagcagagg cagctactgt tgcaacaagc ttggcaagtg
     8521 aaggggcaaa cgttgtgatt ggtcctgcta catcaggagc ttcagcagcc tctattccag
     8581 cattgacatc tgctggtgtt cctatgatta ctccttcagg aactcaaaca aacttggttg
     8641 tgaacgataa aggtgaggtc caagaatact tcttccgtac aacctttaca gatgggtatc
     8701 aaggtcaaat tatggctaaa tacgctactg agaacctgtc agctaagaaa gttgttcttt
     8761 actttgacaa ctcttctgat tatggtaagg gtgtagcaga ggcgttcaag aaagcataca
     8821 caggagaaat cgtttcagaa atcacatttg cttctggcga taaagatttc caagctgctt
     8881 tgacaaaatt gaaagataag gaatttgatg caatcatcat gcctggttac tacaatgaaa
     8941 caggtacaat tgtgaagcaa gcacgcggtt tgggtattga caagccaatt cttggttcag
     9001 atggttttga ctcaccacaa tttactgaat tggcaactgc atcagcagca tcaaatgttt
     9061 actacctttc tggatttgta acatcagcaa gcgaaaaagc aaaagcattc tatgatgcct
     9121 atgtgaaaga atacaatgaa gagccatcaa tgttctcagc tttagcttac gactcggttt
     9181 acatggcagc agaagcagca aaaggtgcag ctgattcagc agcagtgaag gctaatcttg
     9241 cagctctgaa agactttaaa ggtgtgactg gtacaatgtc tgttgacaaa gagcacaatg
     9301 tcattaaatc agtttatgtt gttagtttaa caaacggaga agaatcatct gtagatacta
     9361 tctctattga ctaatagtgg atgaaaagag ggaattccct ctttttcact tgtcagaatg
     9421 tatagaatag tatacatgcg gatataaaat ttgggttggt taaattttct gtaattttaa
     9481 tgattaaact ctctgtctaa cttgcttatt ttcagaaaat tttgtataat gtaacaatgc
     9541 tattgagctc gaaaaatatt tagaaagatt ggtgaaccta tgcttcaaca acttgtcaat
     9601 ggtttgattc ttggttctgt ttatgccctt ttggcccttg gttataccat ggtgtacgga
     9661 attatcaaac tcattaactt cgcccatggt gatttgtata tgatgggtgc ttttatgggg
     9721 tacttcctat taaataattt aaattttatt gcagatggtg gaacgcgttt ctttgttgct
     9781 ctcattctag cgatggtagg gacggctatt ctcggtgtag ttatagagtt tttagcctat
     9841 cgtcctttgc gtaattcaac gcgtatagca gctttgatta cggctatcgg tgtatccttc
     9901 ttcttagaat acactatgat taaattcttt acagcagacg taaaagcctt tccacaagca
     9961 atcgaagctg tgacttttaa ccttggtcca gtttctgtta cgaatatcca gttgattatt
    10021 cttggtgtat cgcttgcgtt gatggttgca cttcaattaa ttgtcaaacg tacaaaaatg
    10081 gggaaagcca tgcgtgcggt ctctgtagat agcgatgcag ctcagcttat ggggatcaat
    10141 gtaaaccgta caatcagctt tacttttgct cttgggtcgg ctcttgcggg tgctgcgggt
    10201 gttctcctag gtctctacta caaccagatt gagcctttga tggggatgac cccaggtttg
    10261 aaagcctttg tagcggcagt attgggcggt atcggtatta ttccaggtgc tgcgcttggt
    10321 ggcttcgtta tcggtatcat cgaaacattc acctatgtga ttgaattcga tactttccgc
    10381 gatgcgatcg tatatactgt tttgatcatc atcctcttgg tgcgtccaag tggtattctt
    10441 ggtaaaaatg tgaaagagaa ggtgtaacca tgaagcaaaa tttaaaagtt aatgcgattt
    10501 ggttggctat cttattggct ggttttggac ttattcaagg tcttgtatca gcagggattc
    10561 tgaattttta ctacatccag attgttcagc aaatcggtat tcaaattatc ttggcagttg
    10621 gtttaaactt gattgttggt ttctcaggtc aattttcact tggtcatgct ggttttatgg
    10681 ctattggtgc ttatgcagta ggtatccttg ggaaaatgat gccaacttat ggtggatttg
    10741 ctatcgcctt gattgttggg atgttaatct caggtgcagt agctctttta gtcggtattc
    10801 caacccttcg tttgaaaggt gactaccttg caattgcgac tctcggtgtt gcagagatta
    10861 ttcgtatttt aattatcaat ggtggtagtg taacgaatgg tgcagcaggt attatgtcta
    10921 ttccactctt tacaagctgg cctttagttt acatttttgt aatcatcact accttggtga
    10981 cagttaactt tttgcgcagt ccgatgggac gtgctacaat ttctgttcgt gaggatgaaa
    11041 ttgcggcaga atctgtaggt gtcaatccaa catttgttaa agtttcagcc tttgtatttg
    11101 gtgctatgac ggcagcaatt gcaggtggtc tccatgcagg atatgttggg acaattgttc
    11161 cgaaagactt tgcctttatg acctccgtta acgttttgat tatcattgta ttgggtggtt
    11221 tgggctctat cacaggtacc tttgtagcag ccattgtact tggtattttg aacgtattcc
    11281 tcaagagtta ctctgatatt tcaatgatta tctattcatt agctcttatt ctcttaatga
    11341 ttttccgtcc tggaggtctt ttgggaacta aggaattcag tgtagcggct ctactcaaga
    11401 agaaggaggt taagtaatgg cattacttga agtaaaagat ttaaccaaga acttcggtgg
    11461 cctgactgcc gttggtgacg tgaccatgga acttcatgaa ggggaattgg ttggtcttat
    11521 cggtccaaac ggtgctggta aaacgactct tttcaacctc ttaacaggtg tttatgagcc
    11581 aagcgagggg acaatttctc ttgcagggac tattttgaac ggtaaagcac catcaaaaat
    11641 tgcttcgctt ggtcttggtc gtactttcca gaacattcgt ttgttcaaaa atatgaccgt
    11701 tttggaaaat gttttgattg gtcttggcaa ccatggtaaa tcagaagtat ttgcaagttt
    11761 cttccgtctt ccagcattct ataagaatga agaagcgttg aaaacaaaag cgattgaact
    11821 tttgaaaatc ttcgatttgg atggagatgc agatacatta gctaagaacc ttccttacgg
    11881 tcaacaacgt cgtttggaaa tcgttcgtgc ccttgcgact gaaccaaaaa ttcttttctt
    11941 ggatgagcca gcggctggta tgaacccaca agaaacggca gaattgaccc aacttatccg
    12001 taaaatcaaa gaagaatttg gcattactat catcttgatt gaacacgata tgagcttggt
    12061 tatggaagta acggagcgga tatacgtatt ggagtacggt cgtttgattg cccatggtac
    12121 accagaagaa attcgtaata acaagcgtgt aattgaagcc taccttggag gtgaagctta
    12181 atggcaatgt tagaagtaaa aaatctttcc gttaactacg gtgttatcga agctgttaaa
    12241 gatgttagct ttgaggttaa tgaaggtgaa gttgtaacac ttatcggcgc taatggtgct
    12301 ggtaaaacct ctatccttcg tactatttca ggacttgttc gtccatcagc tggaacgatt
    12361 tctttccttg gaaatgagat tcaaaaagtt cctgctcgta aaatcgttgc ggacggtttg
    12421 tcgcaagttc cagaaggtcg acatgtcttt gcaggcttga ctgttatgga aaacttggaa
    12481 atgggtgcct tccttcgcaa caatcgtgaa gaaaaccaag caaacttgaa aaaaatcttt
    12541 gctcgttttc cacgtttgga agaacgtaaa aaccaggatg cagctaccct ttcaggtggg
    12601 gagcaacaga tgttggccat gggacgtgcc ctaatgagcc aaccaaagct cttgctcctt
    12661 gatgaaccat caatgggctt ggctccgatc tttatccaag aaatttttga tatcatccaa
    12721 gatattcaaa aacaaggaac aactgtcctc ttgattgagc aaaatgccaa caaagccctt
    12781 gctatcgcag accgtggcta cgtcctagaa acaggaaaag tcgtcctctc aggaacagga
    12841 aaagaactcc tcgcctcaga agaagtcaaa aaagcatatc tgggtggtta aaacgtgggt
    12901 taccccacgt tttaagttgg agatgagaaa cgccggcagg cgtttttctt tatatcaagg
    12961 tctgggggac ctttttaggt tggaaatgag aaccgctggt aagcggtttc ctttataagt
    13021 gggttacccc acgttttaag ttggagatag gaaaagaaaa agtctttaga ggctttttct
    13081 ttgtccccca accctgacgc cttattttag gttggaaata ggaaacgccg ggaggcgttt
    13141 ttctttatat caaggtctgg gggacctttt taggtggaaa tagggagtgt tctctcatgt
    13201 tattgctatc tatgcagtgg tcttcaaata tagtataatg aatgtatatt tggaggtttg
    13261 tatgattaaa gttgaagaga ttttatcgac tctatcaccg aatcaaaagg ttaactatga
    13321 taaagtgttt caaaaaatgg caagcacgtg gcaggaagct gggcttcgac cgaaaattct
    13381 catgcatgtc tgttgtgccc catgctcgac ctatacattg gagtacttga ctcaatatgc
    13441 agatgtaacc atctattttg cgaattccaa tattcatcca aaggcggaat accagcggcg
    13501 ggcagccgtg acggcccagt ttgtcaaaga atttaatcag aaaactggac agtcggtagg
    13561 ctatctagaa gctgactata gccctcagga atttttcaag aaaactcagc atcttgccaa
    13621 tgagccagag ggaggagatc ggtgtaaggt ttgctatgat tatcggttgg ataagactgc
    13681 ggagaaggca gttgagcttg gctttgacta ttttggttcg gctttgacaa tcagtccaca
    13741 taagaattcg cagaccatca atgctgctgg gattgaggtg cagaaattat acggtaccca
    13801 gtatcttccg agtgacttta agaaaaatgg aggctatcag cgttcagttc aaatgtgtga
    13861 agagtatgac atttatcgcc agtgctattg tggctgtgtc tatggggcca atgcccaagg
    13921 tgtcaatctc gtacaggtaa aaaaagaagc cttggaattt ttaaaaacgc acagagatta
    13981 ttcccacatt cctttcaagg tgttggataa gaattgagtt tttgagttta gtttttactt
    14041 aaggagcgtt ttttcttgct ttctctatga aattggtata tactaattcc ggttaatata
    14101 ttgtactact aggagaaaag caaaatgaaa ttgagaaaag tatcgtttat tttagttacc
    14161 tttgtcatcg gtttactatc gttatcaagt gtaaaggcgg agacttcgat gtttgtacca
    14221 gtaggtcaac agaatgttcc gggggtagaa gtagctcctt ttagaactgg ttcagactct
    14281 atccatgtcc atgttggttc atttggttat gttgctacat atataaatgg agaacgattg
    14341 aaggtcgagc ctgtaaagca tgaactcaag tgtccgagtt atactacaga gaattgccaa
    14401 actaaagagt ggtatacaag tggggaacgt gcggacggca gtggagatta tgttgtaaaa
    14461 ttgaataaga aactcgaaga aggtgatgtt gttacattaa aatttgcaga cgatgggaat
    14521 ttgtattttg gtcaattagt atataaaagt gaaaagaagc gagttgaaca gactcaaaag
    14581 gaacaagagg atgagtacgc agatgccttg ttcaaacgtt ctattgagga agaagaaaat
    14641 aagacttgga gagatcggat aaaggatact tttcaagatg cttggtggaa tttcaaaggg
    14701 tggtggaata gctaacaaag gaactgctat ttggaaaata ggtcatttgg agtgattatc
    14761 aattaatttt ttctaccttc ctgatataaa tttcaatgaa agagtccaga ctttatgtct
    14821 aggctctttt tgtggtacaa tgagaaaaga attttgaggt ttttatgaca gtaattgaac
    14881 agattgaaaa attaaaaaaa gagaaattag agcttctgac cgttttatgc ggtgaagaca
    14941 tcggacaata ccaaatagcg aaagacttac ttctgcggaa gcttgatttt gatgcggcag
    15001 atttaggttt tgcctatttt gatatgtcgg aggctgatta tcatcaggta gacttggacc
    15061 tagtttctct tcccttcttt tcagatgaaa aagtagtgat tcttgattat tttgcagatt
    15121 taacgacaga taaaaaacgg catttgacag atgatgaact caaacagttt gaggtctatt
    15181 tggaaaatcc tgtcgaaaca acatgtttgg tcattctagc accgggtaag ttggacagta
    15241 agcgtcgtct ggtcaaactc ctcaaacgag atggactagt attagaagcc aatcctttga
    15301 aggagatgga tttgaaaaat catttccaaa aggagataat gcgtcttgga ttgcagatgg
    15361 gaggagaggt ttttcaatat cttttagaaa agtccaattt tgattttgca gaaatcagta
    15421 agaacctagt ctttcttcaa tcttataagg gaaaagagac gattgaaatt tcagatattg
    15481 atgcggctat ccccaaaaca ctacaagata atatctttga tttaacacag ttaattctac
    15541 aatccaaggt agacgaggct cgcaacttag ttcgtgactt gcggttgcaa ggggaggaag
    15601 aggttaagtt gattgccatc atgttaaccc aatttcgaac ctatctacag gtgcaaatac
    15661 tactagagca aggccgaggg gaacagcaga tagtagctga gttatcaact attatggggc
    15721 gtaaggtgaa tccctaccaa gtaaagtatg ctttgcgtga ttcacgccat ctgtccattg
    15781 gatttttgaa aaaagtcgtc aagctcctaa tcgatacaga ttttcaaatt aaaacaggcc
    15841 aatttgataa ggattattta tttgatttgc tattattaaa aatcgcgact gcatagtaaa
    15901 aagagtattc ttagatcgag ggtcaaccgt ggtttcagac aaataaattg aaaaggctct
    15961 cacattggag tagaatagaa ctatcactta gtataggaga aaacgaaaat ggcaattatt
    16021 ttaccagacc ttccatacgc atacgatgcc ttggaaccac atattgatgc agaaacaatg
    16081 acccttcacc acgataagca ccatgcgact tatgttgcaa atgctaatgc agcacttgaa
    16141 aaacatcctg aaattggcga agacttggta gctcttttgt cagatgtgga gcaaattcca
    16201 gctgacatcc gccaagcttt gataaacaac ggtggtggtc atttgaacca tgctcttttc
    16261 tgggaattgc tttcacctga aaaaacagaa atttcagcag aattggcagc agatattgat
    16321 gcaacctttg gttcatttga tgctttcaaa gaagccttca ctgcagcagc aacaactcgt
    16381 ttcggttcag gttgggcttt cttggttgtt aataaagaag gtaagttgga agttatctca
    16441 actgctaacc aagacacacc aatcatgcaa ggtttgaaac caatcttggc attggatgtt
    16501 tgggaacatg cttattacct caactaccgc aatgtgcgtc caaactacat caaagccttc
    16561 tttgaagtaa tcaactggga caaggttaac gaactttata aagcagctaa ataagaatag
    16621 ttctcttcct ttgggaagag attttttagt tgccaggatt atttttgaga gaattgtgta
    16681 agggaagaag gggaattttc gta
//
LOCUS       DBNFEG010000031        15782 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.031, whole genome shotgun sequence.
ACCESSION   DBNFEG010000031 DBNFEG010000000
VERSION     DBNFEG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 15782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15782
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.031"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..473)
                     /locus_tag="AC1PNG_000686"
     CDS             complement(<1..473)
                     /locus_tag="AC1PNG_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HIP6701206.1"
                     /translation="MKNNSIKTVVATGIGAALFVVIGHLINIPTFVPNTSIQLQYAVQ
                     SLLAVVFGPVVGFLVGFIGHTLKDSLTYGPWWSWILASGVFGLVVGLTKKRLRIQEGI
                     FEGKDILFFNLVQIAANVLAWGVIAPVLDILIYSEAANKVFAQGLVAGIANSITVA"
     gene            complement(494..1342)
                     /locus_tag="AC1PNG_000687"
     CDS             complement(494..1342)
                     /locus_tag="AC1PNG_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM hydrolase/SAM-dependent halogenase family
                     protein"
                     /protein_id="HIP6701207.1"
                     /translation="MSNNLLVLQSDFGLVDGAVSAMIGVALQESRDLVVHHLTHDITP
                     YNIFEGSYRLFQTVEYWPEGTTFVSVVDPGVGSKRKSVVALTEQNHYIVTPDNGTLSF
                     IKKYVGIKAVREISEVANRRANTEHSYTFHGRDVYAYTGAKLASGHISFEEVGPELSV
                     DKIVEIPTVPTEVGSDYVKGAIDILDVRFGSLWTSITREEFYTLQPQFEDRFEVTIYN
                     NDMLVYQNQVTYGKSFADVRIGQPLIYINSLYRVGVAINQGSFAKAYNVGVGQNWHIE
                     IKRISN"
     gene            complement(1774..2490)
                     /locus_tag="AC1PNG_000688"
     CDS             complement(1774..2490)
                     /locus_tag="AC1PNG_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIP6701208.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPETN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRSAFGKNGGNMGASGSVSFMFDKKGVVVFAGDDADAIFELLLEADVEVDD
                     VEAEDGTITVYTAPTDLHKAIVALKESGIQEFNVTELEMIPQSEVSLEGDDLATFEKL
                     YDALEDDEDVQKIYTNVDGF"
     gene            2651..2962
                     /locus_tag="AC1PNG_000689"
     CDS             2651..2962
                     /locus_tag="AC1PNG_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIP6701209.1"
                     /translation="MKRNKHLPLTETTYYILLALLEPAHGYHIMQKVEDMSDGDVKIA
                     AGTMYGAIENLLKLKWICSVPSSDKRRKVYQVTATGQEIIKLETQRIQKLHHIAKELG
                     F"
     gene            2973..3599
                     /locus_tag="AC1PNG_000690"
     CDS             2973..3599
                     /locus_tag="AC1PNG_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HIP6701210.1"
                     /translation="MIRHKLFTSLQEEEHWINSIQSEGYQLVKVTPWTAAYHFEKCSR
                     TPHPVRLDFHEHIAKGEYSNYLSLFEDCGWQPIQGSRRCGIHYFQQTIEASSTEIFSD
                     TESKKAFYSRYQNYAYSYFGLFLTLFFIHYQVGLQNGYTLWNPKSWYLTPELWERTGT
                     SFWFGFLFETPFALFRSGIIPLFFLAWSIYFLHIAEKGKKEAKKLEKY"
     gene            complement(3671..5839)
                     /locus_tag="AC1PNG_000691"
     CDS             complement(3671..5839)
                     /locus_tag="AC1PNG_000691"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HIP6701211.1"
                     /translation="MKKFLSFEFWQKFGKCLMVVIAVMPAAGLMVSIGNSIPLISPES
                     ELLIRIGNIIAQIGWGIIGNLHLLFALAIGGSWAKEKAGGAFSAGLAFILINLITGHF
                     FGVTTDMLADAAATVTTVFGTEIPVSGYFVNILGQPALNMGVFVGIIAGFVGATTYNK
                     YYNYRKLPDVLTFFNGKRFVPFVVIYRSVLVALGLAIFWPVVQTGINSFGKWIASSQD
                     TAPILAPFVYGTLERLLLPFGLHHMLTIPMNYTSLGGTYDILTGAQAGAQVFGQDPLW
                     LAWITDLINLKDAGDMAQYNDLLANITPARFKVGQMIGSSGILMGLTYAMYRNVDEDK
                     KHKYKGMFISSALAVFLTGVTEPIEFMFMFAAMPLYVVYAFVQGAAFAMADVVNLRMH
                     SFGNIEFLTRTPMAIKAGIGMDVINFIWVTALFAVGMYFIANFMIKKFNLATAGRNGN
                     YDTETTDVVSNSNVDTADANSQVVQIINLLGGRDNIADVDACMTRLRVSVKDVAQVGD
                     ENAWKQAGAMGLIIKDSGVQAVYGPKADVLKSDIQDLLESGVAIPRTEIVATEEVAPE
                     TQFKGVTETVYSVAEGQAIAITEVKDPVFSQKMMGDGYAVEPSSGNVYAPVSGIVTSV
                     FPTKHAVGILSDKGVEVLVHVGLDTVALNGAPFSAKVTDGQRVEAGDLLLVADLDAIR
                     SAGRETTIVVAFTNTAEIKSVSLGNLGQVSQDSQVATVEL"
     gene            complement(5873..6664)
                     /locus_tag="AC1PNG_000692"
     CDS             complement(5873..6664)
                     /locus_tag="AC1PNG_000692"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIP6701212.1"
                     /translation="MKLLTINVHAWLEERQDEKLDVLAKTIAEKAYDVIALQEVNQLI
                     TADLVTKDLKADNYGLILLEKLRALGQFDYSYYWSNSHIGYDKYEEGIAFLTKLPVYE
                     VDPFYCSQNKSVDSILSRKIMGLTVFYQDQLIDLYSCHINLPGSEEEDQLDNVRTIVE
                     RTGNRRFKILMGDFNTDAFSDREAYEAITELGLYDSYNLAMEKDQGITVEKAIDGWAG
                     HSQEKRLDYIFLNQKKEVLSSLVIFNGENRPIISDHFGVEVEINV"
     gene            6863..7570
                     /locus_tag="AC1PNG_000693"
     CDS             6863..7570
                     /locus_tag="AC1PNG_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018367901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HIP6701213.1"
                     /translation="MSKYKKVYQDIKKKIEEQVWSTGQALPTENELMEVYSYSKDTIR
                     KALSLLEMDGYIQKKQGKSSIVIEHGLMKDQYLSEIKTAGELNKRSQHQIQTQLTSLY
                     IIQGQEDLMSTFEVDDKVDFYRVSRVRTIDGERLEYDISYFDRRVVPYLSKEIAESSI
                     YRYLEEELHLTISHSRREISFRFANEEEKQLMDLGDYDMVVVVTSITYLSNGQAFQYG
                     TISYRPDKVSFVSMAKR"
     gene            complement(7642..8586)
                     /locus_tag="AC1PNG_000694"
     CDS             complement(7642..8586)
                     /locus_tag="AC1PNG_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HIP6701214.1"
                     /translation="MTYTHLTTNELVMIEAYYKEGIAVSHICQSLKRSRQTIHKVIAY
                     LKAGHTAYDYYKDYKANKKRCGRRQTQLTQSEQDFIQRHLELNWSLDVVKGTYPDKIP
                     CSMRTLYRLADRGIFKKVDLPWKGKRKPNGIAEKRGKQAFRRDIRERSEIYPDFDTEF
                     GHLEGDTIVGKHHKSAVVTLVEKQSKAIITLQTNGRKASDIEQSLNRWLSQFPKHLFK
                     SVTFDCGKEFSNWKTIANQHDIDIFFADPGCPGQRGLNEHSNGLLRRHGLPKQMDFNG
                     IPQKYLSAITDKRNRIPRKSLNYRTPYQVFLSYVKCLA"
     gene            complement(8848..11112)
                     /gene="glgP"
                     /locus_tag="AC1PNG_000695"
     CDS             complement(8848..11112)
                     /gene="glgP"
                     /locus_tag="AC1PNG_000695"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HIP6701215.1"
                     /translation="MTKFTTFAETNTSKKLADLTNEEIYLQLLNYVKDAAASKPKNTG
                     KRKVYYISAEFLIGKLLSNNLINLGVYKDVQAELAAAGKSLAQVEDVEPEPSLGNGGL
                     GRLASCFVDSMSTLAINGEGVGLNYHCGLFRQVFKKNEQEAEPNFWIENDSWLIPTTI
                     SYDVPFKNFTLKSKLDRLDILGYKKETKNYLNLFDIESVNYDLITDGISFDKTDIKEN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLLIDEAVERGSNLHDLADYAYVQINDT
                     HPSMVIPELIRLLTEKHGIEFAEAVAIVKNMTGYTNHTILAEALEKWPLEFLEEVVPH
                     LVDIIKELDALIRAEIKDPAVQIIDESGRVHMAHMDIHFSNSVNGVAALHTEILKNSE
                     LKAFYELYPEKFNNKTNGITFRRWLEFANQDLADYIKELIGDEYLTDATKLEKLLAFA
                     DDKEVHAKLAEIKFNNKLALKRYLKDNKGIELDENSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKNGNLPKRKITVIFGGKAAPAYIIAQDIIHLILCLSELINNDPEVSKYLNV
                     HLVENYNVTVAEKLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGMDNIYTFGKDSDTIIDLYDKAGYVSADYYNGDANIKRAVDFIVSDEVKALGNEE
                     RLGRLHHELISKDWFMTLIDLAEYIEVKEQVFADYEDQDSWNKKVVHNIAKAGFFSSD
                     RTIEQYNQDIWHSN"
     gene            complement(11105..12613)
                     /gene="malQ"
                     /locus_tag="AC1PNG_000696"
     CDS             complement(11105..12613)
                     /gene="malQ"
                     /locus_tag="AC1PNG_000696"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIP6701216.1"
                     /translation="MTNRTSGILMHITSLPGKFGIGTFGQSAYDFVDFLVETKQTYWQ
                     ILPLTTTSYGDSPYQSFSAIAGNTHLIDFDLLVEDGLLATEDFQDVNFGDNPEKVDYA
                     LIYQVRRPILEKAVQAFLQDDKKKAAFLEFEKANSSWLTDYAEFMAIKEYFGNKALQE
                     WEDKKVVARNEEALDKYRLELANQIDYYKVTQYFFFSQWKQLKEYANKHHIKIIGDMP
                     IYVSADSVEVWTKPQLFKLDSERKPLYVAGVPADNFSADGQLWGNPIYDWPEHEKTGY
                     NWWIYRIHESFKLYDVLRIDHFKGFSDFWQVDGKAEVAKVGTWEPGPGYNLFKAVKET
                     LGDLPIIAEDLGNIDAKARKLLADCGYPGMKILEFGFFDVTGKSIDAPHRCIPNSVAY
                     TGTHDNEVVNGWYNNLEPEQQEYVDAYSNRKPIEKVSQAMLRMLFATVSDTAIATMQD
                     VLDLGEESRMNMPSTIGGNWEWRMKAEDLTQERKDFLTKMTLLYQRGNENHD"
     gene            complement(12795..14894)
                     /locus_tag="AC1PNG_000697"
     CDS             complement(12795..14894)
                     /locus_tag="AC1PNG_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIP6701217.1"
                     /translation="MNNNFNNFNNMDDIFNQLMGNMGGYSTERRRYSINGREVTPEEF
                     AMYRQTGRLPQTEEVAQTQAKGQIKSDGILAKLGRNLTQDAREGKLDPVIGRNKEIQE
                     TAEILARRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGLEA
                     GTQYRGAFEENVQNLVDEVKKAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKPAL
                     SRGELTVIGATTQDEYRNTIHKNAALARRFNEVKVNAPSAEDTYQILKGIKPLYEAHH
                     NIELPDEVLRAAVDYSVQYIPQRSLPDKAIDLIDVTAAHLAAQHPVTDIQTLEAEMAE
                     AKQLQLEAAEKEDYEKALNEKVRIDKLQKQIDNHTEQQKVVATVNDVAQAVERMTGIP
                     VSQMGASDIERLKELKNRLAANVIGQDDAVEAVSRAIRRNRAGFDDGNRPIGSFLFVG
                     PTGVGKTELAKQLALDLFGNKDAIIRLDMSEYSDRTAVSKLIGTTAGYIGYDDNSHTL
                     TERVRRNPYSIVLLDEIEKADPQVITLLLQVLDDGHLTDGQGNQVNFKNTIIIATSNA
                     GFGYGMAEGEENQDIMDRIAPFFRPEFLNRFNAVIEFKHLDKDDLKAIVELLLAQVNN
                     TLAKKGIQLTVTEAAKGFLMEEGYDKAMGARPLRRVIENQIRDKVTDYYLDHVDVKYL
                     EADVVDGSIQIKEQSFA"
     gene            complement(15309..15719)
                     /locus_tag="AC1PNG_000698"
     CDS             complement(15309..15719)
                     /locus_tag="AC1PNG_000698"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIP6701218.1"
                     /translation="MIQPIMKDIFFLQQKSEPATQLDVQVGQDLQDTLSANAHACVGM
                     AANMIGIKKRIIIVNMGFTNLVMYNPVLISKAKPYQTEEGCLSLEGTRPTTRYQEIEV
                     EFLDASWKKISLKLTDFQAQIVQHELDHLEGIII"
BASE COUNT         4553 a         3404 c         3009 g         4816 t
ORIGIN      
        1 gcaactgtaa tactgtttgc aatacctgcc acaagtccct gtgcaaaaac cttattcgcc
       61 gcctcactgt agataagaat atctaaaact ggggcaatga ctccccaagc aagcacattc
      121 gccgcaatct gaacaaggtt aaagaaaaga atatctttcc cttcaaaaat cccttcttga
      181 atacgtagac gttttttggt aagaccaaca accaaaccaa agacccctga tgccaaaatc
      241 catgaccacc atggaccgta agtcaacgaa tccttaagcg tgtgcccaat gaaaccgacc
      301 aagaagccaa ctacaggacc aaatacaact gccaatagcg attgaacagc gtattgaagc
      361 tggatagaag tatttgggac aaaggttgga atattgatta aatgaccaat aaccacaaag
      421 agggctgcgc cgataccagt agcaacgact gttttgattg aattattttt cataactatc
      481 tcctaaaatt tctttaatta ctaatacgtt tgatttcaat atgccagttt tgacctacac
      541 cgacattata agccttggca aatgagccct gattaatcgc aacacctaca cgatagaggg
      601 aattgatata aatcaatggc tgtccgatac gtacatccgc aaaggacttc ccgtaggtta
      661 cttggttttg atacacaagc atgtcgttat tgtatatggt cacttcaaag cgatcttcaa
      721 actgcggttg caaggtgtaa aattcttccc tcgtaatcga ggtccaaagc gagccgaatc
      781 gaacatccag aatatcaatt gcacctttta catagtccga accaacttcc gttgggactg
      841 ttggaatttc cacaatctta tccacgctta attctggacc aacttcttca aagctaatgt
      901 gtccagaagc caatttagct cccgtatagg cataaacatc tcgaccatgg aaggtgtacg
      961 aatgttctgt attggcacgg cgattggcca cctctgaaat ttctcgaacc gcctttatcc
     1021 ccacatattt tttgatgaag gatagcgtcc cattatctgg cgttacaatg tagtgatttt
     1081 gttcggtcaa agctactaca ctcttacgtt ttgaaccaac tcccggatca acaaccgaaa
     1141 caaatgttgt tccctccggc cagtattcga ccgtttgaaa gaggcgatat gaaccctcaa
     1201 agatattgta cggagtaata tcatgagtta gatggtgaac aactaaatct ctcgattctt
     1261 ggagagcaac accaatcatc gctgaaactg caccatcaac taaaccaaaa tctgattgta
     1321 aaacaagtaa attatttgac atacattctc ctaatcttat taccgttcta tgataacaga
     1381 aaacctgtga ccattcaaat agatatttcc aaagcctggc tatagttttt ccttatacct
     1441 catctatcaa gcactctact atcagccacc gaaaactcgc aaatttttcc atcgtacctt
     1501 cttcgaactt aagctaaaat atccccactt ctctagttcc aacaactatc gtctttgagt
     1561 aattggatat aaagaaaacc gcctagcgca gttccaattt ccaaactttt aagccaaaaa
     1621 ttgtccttgt ggacagctga aaataaagaa aaccagctac cgctggttct catttccaac
     1681 cttcgacgtc ccacgtacaa tgagaatcat ctaccgacga tttcaatttc cagcctttta
     1741 gtataaaaaa agtgctacca gcactttttt atactaaaac ccatccacat tcgtgtagat
     1801 tttttggacg tcttcgtcgt cttctagggc atcgtagagt ttttcaaatg ttgcgaggtc
     1861 atcgccttca agtgatactt ctgattgtgg aatcatttca agttcagtga cgttgaactc
     1921 ttggattcct gattctttaa gtgccacgat tgccttgtgc aaatcagttg gggctgtata
     1981 gacagtgatt gtgccgtctt ctgcttctac gtcatccact tcgacatctg cttcaaggag
     2041 caattcgaag atggcgtctg catcatcacc tgcaaagaca accacacctt ttttatcaaa
     2101 catgaatgat acagaaccag atgcccccat gttaccgccg tttttaccaa aagcagagcg
     2161 cacgttagct gccgtacggt ttacgtttga tgtcaaagta tcaacgataa tcatagaacc
     2221 atttggtcca aagccttcgt aacgaccttc cacgaaggtt tcgtctgtgt ttccttttgc
     2281 cttgtcaatg gccttgtcaa tgatgtgttt tggaacctgc gcttgcttag cacggtcgat
     2341 aacaaatttc agcgctgagt ttgtttctgg atctgggtca ccctttttcg ctgctacata
     2401 gatttcaaca ccaaatttgg cgtaaacttt tgagttagca ccgtcttttg cggttttctt
     2461 ggctacaata ttggcccatt tacgtcccat ggggaacctc ctacatttta taaaatcgct
     2521 ttattatacc ataaatggca aggattgaga agatttttaa aatcttcttc actatacagt
     2581 tgacatgtat agtttctcga tatataatat atagtacaac tatacatttg aactatatag
     2641 gagaagccaa atgaaaagga acaagcatct ccccttaacg gagacaacct actatattct
     2701 attagctctc ttggaacctg cccatggcta ccatatcatg caaaaagtag aggatatgag
     2761 tgatggtgat gtgaaaatcg ctgcaggaac catgtatgga gcgattgaga atcttttgaa
     2821 actaaaatgg atttgttccg ttccaagttc cgataaaaga agaaaagtct atcaagtaac
     2881 agcaacgggc caagagatta ttaaactgga aacacagcgt attcaaaaac tacatcatat
     2941 tgccaaagaa ttaggttttt aaggaggaag ccatgataag acacaaatta ttcaccagtt
     3001 tacaagagga agaacattgg attaattcga ttcaatccga aggttatcag cttgtcaaag
     3061 taactccgtg gacagcagcc tatcattttg aaaaatgtag tcgaacccct catcccgtac
     3121 gccttgattt tcatgaacat attgcaaaag gagaatattc aaattacctc tctctatttg
     3181 aagactgtgg ttggcaacct atacaaggta gtcgtagatg tggcatccat tattttcagc
     3241 aaacaatcga agcttcaagc actgaaattt tttctgatac cgaatcgaaa aaagcattct
     3301 attcacgcta ccaaaattat gcctactcct atttcggatt atttctgact ttatttttta
     3361 tccattacca agttggactc caaaatggat acacactttg gaatcctaaa tcctggtacc
     3421 taactccaga actatgggaa cgaactggta ccagtttttg gtttggtttt ctctttgaaa
     3481 ccccattcgc attgtttaga agtggcatta ttcccttatt tttccttgct tggtctattt
     3541 atttcctcca tatcgctgaa aaaggtaaaa aagaagcgaa gaaattagaa aaatactgat
     3601 ggcatactat ccaaaagaaa aagattgaaa agagttgact acaatcatct tttcaatctt
     3661 tttttacttt ttacaactca actgtcgcaa cttgactatc ttgactaacc tgtccaagat
     3721 ttccaaggct gacagatttg atttcagcag tatttgtgaa ggcaacaacg atggttgttt
     3781 cacgtcctgc tgagcgaatg gcatccagat ctgcgacaag gagcaagtct cctgcttcaa
     3841 cgcgttgccc atctgttacc ttagctgaga atggggcacc gtttagtgca accgtatcta
     3901 ggccgacgtg caccaagact tctacaccct tgtcagacaa aatcccgaca gcgtgtttgg
     3961 ttgggaagac actggttacg atacctgaaa ctggtgcgta aacattgccg gagctaggct
     4021 caactgcata accgtcgccc atcatttttt gtgagaagac cggatctttc acttctgtga
     4081 tggcaatggc ttgcccttca gcaacagagt aaactgtctc cgtcacacct ttgaactgag
     4141 tctcaggagc aacttcttct gtcgcaacaa tttccgtacg agggatggct acgcctgatt
     4201 ctagtaagtc ttgaatgtct gatttgagaa catctgcttt tggtccgtag actgcttgaa
     4261 cacctgagtc tttgataatc aagcccatag caccagcctg tttccaagca ttttcatctc
     4321 caacctgtgc cacatcttta acactaacgc gaagacgagt catacaagca tccacatctg
     4381 cgatattatc acgaccacca agcaagttga tgatttgaac cacttgtgaa ttggcatccg
     4441 cagtatctac gtttgagttt gaaaccacat ccgttgtttc tgtatcatag ttaccattgc
     4501 gtccagctgt tgctaggttg aatttcttaa tcataaagtt ggcaatgaag tacataccaa
     4561 ccgcaaagag ggctgttacc cagataaagt taataacatc cataccgata ccagctttga
     4621 tcgccatcgg tgtacgtgta aggaattcga tattaccaaa tgagtgcata cgcaagttga
     4681 caacgtcagc catggcaaaa gccgcaccct gtacaaaagc ataaacgaca tagagtggca
     4741 ttgctgcaaa catgaacata aactcaattg gttctgttac acctgtcaag aatacggcaa
     4801 gtgctgaaga aataaacata cctttgtact tatgtttttt atcttcatcc acattgcggt
     4861 acatagcgta agtcaatccc atcaaaatcc ctgatgaacc aatcatttga ccaactttga
     4921 aacgggctgg agtaatgttt gcaagaaggt cattgtactg agccatatca ccagcgtctt
     4981 tgaggttgat caagtctgta atccaagcca accagagcgg atcttgtcca aatacttgtg
     5041 cacctgcttg tgcacctgtc aagatgtcat aggtaccacc aagtgatgta tagttcattg
     5101 gaatggtcaa catgtggtgc aaaccaaatg gaagaagcaa acgttccaag gtaccataaa
     5161 caaaaggtgc aagaattggt gctgtatcct gtgagcttgc aatccactta ccaaagctgt
     5221 tgattcctgt ttgaacaact ggccaaaaga tagctaatcc tagcgctaca agaactgagc
     5281 gatagataac cacaaatggt acgaagcgtt taccgttaaa gaaggtcaaa acatcaggta
     5341 atttgcggta attgtagtat ttgttatagg ttgttgcccc tacaaaacca gcgatgatac
     5401 caacgaagac acccatgttc agagcaggct gacccaagat gttgacgaag tagcctgaaa
     5461 ccggaatctc tgttccaaaa acagtggtta cggttgccgc tgcatctgca agcatgtcag
     5521 tcgtcactcc aaagaagtga cctgtaatca agttaataag gatgaaggca agacctgctg
     5581 agaaggcacc accagccttt tccttagccc agctaccacc gatagccaag gcaaagagca
     5641 aatgaaggtt tccgataatc ccccaaccga tttgggcgat aatattcccg atacgaatca
     5701 agagttctga ttctgggctg attaacggaa tagagtttcc gatagaaacc atcagacctg
     5761 ctgcaggcat aactgcaatc acgaccatta aacacttacc gaatttttgc caaaattcga
     5821 aactaagaaa ttttttcatt ttttctcctt tattttttgg ctctgtgcca ttttatacat
     5881 taatttcaac ttccacacca aaatggtctg aaataatcgg acgattctct ccattgaaaa
     5941 tcactaagct ggataaaact tcctttttct gatttaagaa aatgtaatcc aatcgttttt
     6001 cctgactgtg accagcccag ccatcgatag ccttttcaac cgtaatcccc tggtcttttt
     6061 ccatcgctag attgtatgaa tcataaagcc cgagctctgt aatcgcttcg taggcctcgc
     6121 gatctgagaa ggcatcggta ttaaaatctc ccatgagaat cttaaaacgt ctattgccag
     6181 tccgttccac aatcgtacga acgttatcaa gctggtcttc ctcttcactc cctggaagat
     6241 tgatgtgaca agaatacagg tcgatcaact ggtcttgata gaagaccgtc aagcccataa
     6301 tttttcgtga taggatggaa tcaacagatt tattctgact acaataaaat ggatccactt
     6361 cataaactgg caacttggta agaaaggcaa tcccttcttc gtacttatcg tagccgatat
     6421 gtgagttgct ccaataatat gaataatcaa attgtcccaa ggctcttaat ttttctaata
     6481 aaatcaagcc ataattatcc gccttcaagt ctttcgttac caaatcagca gttatgagct
     6541 gattgacctc ttgaagagcg atgacatcat aagccttttc agctattgtt ttggctaaaa
     6601 catctagttt ttcatcctgc ctttcttcta accaggcatg gacattaatt gttagtaatt
     6661 tcattgctcc ccctctctct gaaaatgcgt aaccgttttc ttttatagaa atagtataac
     6721 gtaaacgttt gcattagtca atagcttttc cgtaatattt caaactttta cgtaaaagtt
     6781 gtgtattcta ctaaataaaa tagatgtatt gcccctttct ggctctttta tgatacacta
     6841 atgggtagaa agacaggaaa ctatgagtaa gtataaaaaa gtttatcaag atattaagaa
     6901 aaaaatcgaa gagcaggttt ggtcaactgg acaggcccta ccgacagaaa atgaattaat
     6961 ggaagtgtat tcctactcca aggacaccat tcgcaaagca ctatctcttc ttgaaatgga
     7021 tggttatatt caaaagaaac aggggaaatc ttctattgtt atcgaacatg gtctaatgaa
     7081 ggaccaatat ctttctgaaa ttaaaacagc gggcgaattg aataaaagat cacagcacca
     7141 aattcagacc cagcttactt cgctttatat tattcaaggc caagaagatt taatgtcaac
     7201 tttcgaagtt gatgataagg tagatttcta tcgagtgagt cgggtacgga caattgacgg
     7261 tgagcggttg gaatatgaca tttcctattt cgaccgtagg gtcgttccat atctcagtaa
     7321 agaaattgcc gaaagctcta tttaccgcta cttagaagaa gaactccatt taacaatttc
     7381 tcattccaga cgtgaaatct ccttccgttt tgcaaacgaa gaagaaaaac aattgatgga
     7441 cttaggagac tatgacatgg tcgtcgtggt cactagtatc acctatcttt caaatggtca
     7501 agctttccag tatggaacaa ttagttaccg tccagacaaa gttagctttg tatcaatggc
     7561 gaaacggtaa aaggaaactt tcgatttttt tgaccaagtg tacaaaactt ctagaatttt
     7621 atagttaccc aaattgtcaa attaagctag acattttacg taactcagaa aaacctgata
     7681 tggtgttcga taatttaaag atttcctagg aattctattt cgtttatcag tgatagctga
     7741 taaatatttc tgaggaatac cattgaaatc catttgcttt ggtagtccat gacgtcttaa
     7801 taaaccattt gaatgttcat ttaacccacg ttgaccaggg caacctggat ccgcaaagaa
     7861 aatatcaatg tcgtgttggt tggctattgt cttccaatta gagaattctt tcccacaatc
     7921 aaaagtgaca gatttaaaga gatgttttgg gaattgtgat agccatctat ttagtgactg
     7981 ctcaatgtcg ctagcttttc gaccattggt ttgaagtgtg ataatagctt tagattgctt
     8041 ctccaccaag gtgacaacag cacttttgtg atgtttacca acgattgtat ctccttcaag
     8101 atgtccaaat tctgtatcaa aatctggata gatttctgaa cgctcacgga tatccctcct
     8161 aaaagcttgt ttgccacgtt tttcggctat accatttggt ttgcgttttc ccttccatgg
     8221 taaatcaact ttcttgaaga taccacggtc agctaggcga taaagagttc tcatagaaca
     8281 agggatttta tcgggataag ttcctttgac aacatccaaa ctccaattaa gttctaaatg
     8341 cctttgaata aaatcttgtt cactttgagt gagttgggtc tgacgacgac cacaacgttt
     8401 cttgttagct ttgtagtcct tgtagtaatc ataagcagtg tggccagcct tgagataagc
     8461 aatgacctta tgaatcgttt gtcttgatct tttgagggat tgacaaatat ggctaacggc
     8521 tattccttct ttgtaataag cctctatcat gacaagttca tttgtggtaa gatgggtata
     8581 ggtcatttat gttagttcct ttcagacaaa tgtggtgttt atctgagcct aacataaatg
     8641 gcttttttct gtctagctta attttacaaa ttaggttata aaaagaatgt ctccgctttt
     8701 gcagagacat tctttgcatt tatatactaa ggagagcacc aataatggta aaaactggta
     8761 gtttttattc ttcaacacat aggaagaatt gatctgataa gttttggagt ggttcaatca
     8821 actttcctac taattgttgg aattcaatta gttactgtgc cagatgtctt gattgtactg
     8881 ttcaatggta cggtcagatg agaagaatcc agctttggca atgttatgaa caactttctt
     8941 gttccatgaa tcttgatctt cgtaatctgc aaagacttgt tcttttactt caatgtattc
     9001 agccaagtca atcaaggtca tgaaccagtc tttcgaaatc aattcatggt gcagacgacc
     9061 aagacgttct tcgttaccaa gtgcttttac ctcatcgcta acgataaagt caactgcacg
     9121 tttgatattg gcatcgccat tgtagtagtc cgcagataca tatccagcct tatcgtacaa
     9181 gtcgatgatg gtatctgaat ctttaccaaa tgtatagatg ttgtccatac cagccaactc
     9241 agcaatctca acgttggcac catccatggt accaagtgtc aacgcaccgt tgagcatgaa
     9301 tttcatgtta ccagtacctg acgcttcttt agaagccaat gagatttgtt ctgaaatatc
     9361 tgttgcaggg atgagtttct cagcaactgt tacgttatag ttttccacca aatgaacgtt
     9421 gaggtacttg ctcacttctg ggtcattgtt aatcaactct gacaagcaaa gaatcaagtg
     9481 aatgatgtct tgtgcaatga tgtaggcagg tgccgcttta ccaccgaaga taacagtgat
     9541 tttacgtttc ggaaggttac cattcttgat ttcaaggtat ttatggataa catacaaggc
     9601 attcatttgt tggcgtttgt actcgtggaa acgtttgatt tgtgtatcga tgatagagtt
     9661 ttcatccagc tcgatacctt tattgtcttt aaggtaacgt ttgagagcca acttattgtt
     9721 gaacttgatt tctgccaact tagcatgcac ttccttgtca tcagcaaagg caagcaattt
     9781 ttcaagttta gtcgcatctg tcaagtattc atcgccaatc aattctttga tgtagtcagc
     9841 gaggtcttgg ttggcaaatt ccaaccaacg gcggaaagtg ataccgtttg ttttattgtt
     9901 gaatttttct gggtaaagct cgtagaaagc tttcaattca gagtttttga ggatttctgt
     9961 gtgaagcgca gctacaccgt tcactgagtt agagaagtgg atatccatgt gagccatgtg
    10021 tacacgacct gattcatcaa tgatttggac tgctgggtct ttaatttctg cacgaataag
    10081 ggcatccaat tctttgatga tatcaaccaa gtgtggcacc acttcttcaa ggaactcaag
    10141 tggccatttt tcaagtgctt ccgccaagat agtgtggttt gtgtaaccag tcatattctt
    10201 aacgatggca actgcttccg caaattcaat accatgcttt tcagtcaaaa gacggatcaa
    10261 ctcaggaatc accattgatg ggtgagtatc gttgatttgc acataagcgt agtcagccaa
    10321 gtcatgcaag tttgaaccgc gctcaactgc ttcatcaatc aaaagttgag cagcatttga
    10381 taccatgaaa tattgttggt agatacggag caattcacca ttcttatcag aatcatctgg
    10441 gtacaagaag agagtcaagt tttctttaat atcagtcttg tcaaatgaaa taccgtctgt
    10501 aatcaagtca tagtttactg actcaatatc aaacaaattg aggtagttct tagtctcttt
    10561 tttatagcca agaatatcaa gacgatccaa ttttgatttc aaagtgaagt ttttgaatgg
    10621 aacatcatag ctgatagttg ttggaatcaa ccaagaatca ttttcaatcc agaagtttgg
    10681 ctctgcttct tgctcatttt tcttaaatac ttggcggaaa agaccacagt ggtagttcag
    10741 accaacacct tcaccgttga tagcaagtgt tgacatagaa tcaacgaaac atgaagccag
    10801 acgtcccaaa ccaccgttac caagtgatgg ttctggttca acatcttcaa cttgcgccaa
    10861 tgatttacca gctgctgcca attctgcttg aacgtctttg tacacgccca agttaatcaa
    10921 gttgtttgac aagagtttac cgataaggaa ctcagctgag atatagtaaa ctttacgctt
    10981 tcctgtgttt tttggttttg aagctgctgc atcttttacg tagttcaaca gttgaagata
    11041 gatttcttca tttgtcaaat cagctaattt cttcgaagta tttgtttctg caaatgttgt
    11101 aaatttagtc atggttttca tttcctcgtt ggtatagtaa tgtcattttt gtcaagaagt
    11161 ctttgcgttc ttgggtcaag tcttcagctt tcatgcgcca ttcccagttt ccaccgatag
    11221 ttgacggcat attcattcga ctttcttcac ccaagtctag gacgtcttgc atggttgcaa
    11281 tagctgtatc actcactgtt gcaaacagca tacggagcat agcttggcta actttttcaa
    11341 tcggcttacg gttgctgtag gcgtccacgt attcttgttg ttctggctca agattgttgt
    11401 accagccatt gacaacctca ttatcgtgag ttccagtgta ggcaactgaa tttggaatgc
    11461 aacggtgtgg agcatcgata ctcttacctg ttacatcaaa gaaaccaaat tctaagattt
    11521 tcatgcctgg atagccacaa tccgcaagca atttgcgagc tttagcgtca atgtttccaa
    11581 ggtcttctgc aatgattgga aggtctccaa gtgtttcttt gacagccttg aagagattgt
    11641 agccaggacc tggttcccaa gtacctactt ttgccacctc agccttacca tcgacttgcc
    11701 agaagtctga gaaacctttg aagtggtcaa tccgaaggac atcataaagt ttgaaacttt
    11761 catggatacg ataaatccac cagttatagc ctgttttttc atgttctggc caatcgtaaa
    11821 taggatttcc ccataattga ccatctgccg agaagttatc agctggtaca ccagctacat
    11881 aaagcggctt gcgttcactg tccaatttga atagttgggg ttttgtccac acttcaacgc
    11941 tatcagcaga aacatagatt ggcatgtctc cgatgatttt gatgtggtgt ttgttggcat
    12001 attctttcaa ttgtttccat tggctgaaga agaaatactg tgtgactttg tagtagtcga
    12061 tttgatttgc caattctaag cggtatttat caagagcttc ctcgttgcga gctacaactt
    12121 ttttatcttc ccattcttgg agagctttat taccaaagta ttccttgata gccatgaact
    12181 ccgcataatc tgtcaaccaa gatgagttag ccttttcaaa ctctagaaaa gcagctttct
    12241 tcttatcatc ttgtaaaaat gcttgaactg ccttctccaa aattggtcgg cgaacttgat
    12301 agataagagc atagtctact ttttcaggat tatctccaaa atttacatct tggaaatctt
    12361 ctgttgccaa taagccatct tcaaccaaga ggtcaaagtc tatcaagtgt gtatttcctg
    12421 caatagcaga gaaagactgg taaggagaat ctccatagct tgtagttgtt agtggtaaaa
    12481 tctgccagta ggtttgcttg gtttccacca agaaatctac aaaatcatag gcagactgtc
    12541 caaaagtacc aataccaaac ttacctggaa gtgaggtaat atgcatcaaa attccactgg
    12601 tacgatttgt cataaaaacg ttttcctttc tgtcggttac gaatatagtt taacgcaaac
    12661 gtttgcactt gtcaaggatt ttataaaaaa agttttaatg tactctagaa agcctatttt
    12721 atagcatttg ttagcgaata acaaaaaaga aaaatcagat atttctatcc gatttttcaa
    12781 attcttcact atttttaagc aaagctttgc tcttttattt ggatgcttcc atcaaccaca
    12841 tccgcttcca gatatttgac atcaacatga tccaaatagt aatctgttac cttgtctcga
    12901 atctgatttt caatcacacg acgcagagga cgggctccca tggccttgtc atagccttct
    12961 tccatcaaga atcctttggc ggcttccgtt accgttaatt ggatgccttt cttcgccagg
    13021 gtattattga cctgggccaa gagtaattct acaatggctt tgagatcatc tttatccaag
    13081 tgcttgaatt cgataactgc gttaaaacga ttaaggaact ccggacggaa gaatggtgcg
    13141 atacggtcca tgatgtcttg attttcttca ccttctgcca ttccataacc aaaacctgca
    13201 ttagaagtcg cgataataat ggtattttta aagttaactt ggttgccctg tccatcagtc
    13261 aaatgcccgt catctagcac ttgcaagaga agggtaatca cttgtggatc agctttttca
    13321 atctcatcca gaagaacgat tgagtacgga ttgcggcgga cacgctcggt aagagtatgg
    13381 gaattgtcat cgtagccaat gtagccagct gtggtaccga ttaatttgga aacggctgtc
    13441 cgatcgctgt attcagacat atccaaacga ataatggcat ccttgttacc aaacaagtct
    13501 agtgctagtt gcttagccag ctctgtctta cccacacctg tcggaccgac aaagaggaag
    13561 gaaccaatcg gacggttgcc atcatcaaaa cctgcacgat tccgacgaat ggcacgagat
    13621 acagcttcca ccgcatcgtc ttgaccgata acgttagctg ccagacgatt tttcaattcc
    13681 ttgagtcgtt caatgtcaga agctcccatt tgagagactg gaattcctgt catccgctca
    13741 accgcctgag ccacatcatt aacagtcgcc accactttct gttgctccgt atgattatca
    13801 atctgttttt gtagcttatc aatacggact ttttcattca aggctttttc ataatcctct
    13861 ttctcagccg cttccaactg caactgtttt gcctcagcca tttctgcttc caaggtctga
    13921 atatctgtca caggatgttg tgcagccaag tgtgcagcgg tcacatcgat caggtcaata
    13981 gccttatcag gcaagctacg ttgtggaata tattggacag aataatcaac cgcagctctc
    14041 aaaacctcat ctggcaattc aatattgtgg tgggcttcat aaagcggctt aatccctttc
    14101 aaaatctggt aggtgtcttc tgcggacgga gcattaacct tgacttcatt gaaacgacga
    14161 gccagtgcgg cgttcttatg aatggtattg cggtattcgt cctgtgtagt ggcaccgata
    14221 accgtcaatt ccccacggga taaagctggt ttgagaatat ccgctaaacc cttggaacct
    14281 gagtctccgc cagtgctacc agctcccaaa atctgatgaa tttcatcaaa gaagagaatg
    14341 atatttccag cttttttgac ttcatccaca agattttgca cattttcctc aaaagctcca
    14401 cggtactggg tccctgcctc caagccagaa atgtcaatgg agatgatttc cttgttctta
    14461 atggctgctg gcacatctcc gttgacaatg gcttgagcca atccttctac taccgcagtt
    14521 ttaccaacac cagcatcacc gaccaaaaca ggattatttt tcgtacggcg agccaaaatt
    14581 tccgctgttt cttggatttc cttgtttcgt ccaatgactg gatccaactt accttcacgc
    14641 gcatcttggg tcaaattgcg ccccagtttt gcaagaatcc catctgactt tatctgacct
    14701 ttagcctgtg tttgagccac ttcttcggtc tgtggcaggc gaccagtctg acggtacata
    14761 gcgaattcct ccggtgtcac ttcacgccca ttgatagaat agcgacgtcg ttctgtactg
    14821 tatccaccca tgttccccat aagttgattg aaaatatcgt ccatattgtt aaaattattg
    14881 aaattgttgt tcatagtagt tacctcgttt aagtttcagt tatttttgac ttttactgac
    14941 ctttgtcaga aaataaaatc taatagggta taatcctgtc tttcatagca acctcctcag
    15001 aagtttgtct tttactgacc aataggttca agagtaagtc aaaccgttct cgtttgacct
    15061 aaactgacct tttgttcaaa gaatttgtaa ggccctttac cttacgaatc ttattttaca
    15121 tcattggtca gtaaaagtca atgattttgt ttgacctttt ttgacttttt atagtcgaat
    15181 gaattagctt tcagacaagg aactgaggcg caggctatac taaagtatgg caaggcaaaa
    15241 gtgacgatgt atcaaagtta attcaaatga ctataaaaaa atctacctga cggtagattt
    15301 ggtagctact agataataat cccttccaga tgatctagtt catgctgaac aatctgggct
    15361 tggaagtctg tcagctttag gctgatcttt ttccaagaag catccaaaaa ctccacttca
    15421 atctcctgat agcgtgtggt cgggcgagta ccttccagcg acaggcagcc ctcttctgtc
    15481 tgatagggct tggccttgct gatgaggacg ggattgtaca tgactaggtt ggtaaaaccc
    15541 atattgacga tgataatccg ctttttgatg ccaatcatgt tggctgccat gcccacgcag
    15601 gcatgagcgt tggctgacaa agtatcctgc aaatcctgac cgacctgcac atctagctga
    15661 gtcgcaggct cagacttctg ctgcaagaag aaaatatctt tcataatagg ttgaatcatg
    15721 gggtagctcc ttgctttgtt atgaatatgt actttctatt aatcggagag tatcgttatc
    15781 aa
//
LOCUS       DBNFEG010000032        15574 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.032, whole genome shotgun sequence.
ACCESSION   DBNFEG010000032 DBNFEG010000000
VERSION     DBNFEG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 15574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15574
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.032"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            5..1273
                     /locus_tag="AC1PNG_000699"
     CDS             5..1273
                     /locus_tag="AC1PNG_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP6701219.1"
                     /translation="MKKLIHNKLFLSSFVADLISNFGDTLYYLALMNHVLLLADTKFA
                     LAMITASETLPILAGLFIGIWADKTKNKLDTILATLVVRVGLYAFVGFLMGFAPALWV
                     VAVVCVINFLSDLAGQYENGLYMPVSLRVVAAEDRETAMAFKMTVKSLLQIAFQASGA
                     ILIGFMSYQNLAFFNAGTFLVSLAIMATLRPALAKLLKENPIQEAEQTETEAGLIKGM
                     RNSLKESYQAVQNILVLKASIVTIASLNAIFTALSPLVILNMKEFSDFVIVNAGTTVA
                     LISIFFSVGSILGSSIGMALFKNVSLINLLKFSTLMPVLIFSGFFLHNIYVVLTVIFV
                     TAMTLGVFNPKMNALVLNELPEDKLATVGGGIDAFCQIGMVAGQALVALMVTILSVTS
                     ISLIFLLLSVGLVSYTILVGCKPKNEVAQV"
     gene            1285..2325
                     /locus_tag="AC1PNG_000700"
     CDS             1285..2325
                     /locus_tag="AC1PNG_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIP6701220.1"
                     /translation="MKLDYRDYRSEIVEKLFIPLIVKEREEPFEADLSQKEKDILKDA
                     LDIRNEVENKLADFRQEVNEVFVWGHIFTILHTLYFYLLDQGQDPKTVEEACQLILTL
                     SQEEVEDAMRTMLASENDGHREKDLSLMELLEKTDKKPAEKWYWSLALRNPLETVERS
                     VDLLNKLLPIYQPYFEGARAEREAFARDFDIEQLYRESKQLAMTSLDSLGVETAQFFV
                     LSPWNYWFAYYGNEQFDYMKVALLASCRIDQIMLSNDELDLDDLTTALKVISDSTRYQ
                     VLVELTKPHAKSKDIAERMAITGAAVSFHTQKLINGDLLLFNTKNSDVKYSVNRELLQ
                     EVIAKLTEDFDL"
     gene            complement(2462..3451)
                     /locus_tag="AC1PNG_000701"
     CDS             complement(2462..3451)
                     /locus_tag="AC1PNG_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIP6701221.1"
                     /translation="MQNKTKQLNTSLLSLIQFIGAILVVALHCRRLFEADQLHFIQKS
                     IFSRMVVPYFMVTASFFLRRNYPSLSKQYLIRYSKQYLTWSILYLPFYLIYLTLQEIP
                     FLYYPLALLVGLTYTGTSYQLWYMPAFFLGLILVHLSLEKWGWRITACLACLLYLLGS
                     VETYSAYLAESIFLTAFDNYKTFFFTSRNGLFYAPIFVLIGFYLANKLNHPIFQHRQK
                     TKLLVCTALLAFEATVIYLNQGYDKNFLFSLIPFTAYLVAWTLTTDLFRKKKFQFLKE
                     YASYYYFIHVIPVEISFFFLETSSLTKIQQGWLVFLITIITCQLLSWLIINTQ"
     gene            3640..4326
                     /gene="rnc"
                     /locus_tag="AC1PNG_000702"
     CDS             3640..4326
                     /gene="rnc"
                     /locus_tag="AC1PNG_000702"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIP6701222.1"
                     /translation="MKDLHAKLLADFGIDFSDLNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLMISKYLYQKYPDRPEGEMSKLRSTFVREESLAGFSRACGFDRFLRL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKGEKTVEEFIHKVMIPRLEKGNFERVTD
                     YKTALQEILQVNGEIAISYQVIAESGPAHDKTFEVEVSADRRVIGRGRGRSKKLAEQA
                     AAKNAVEARG"
     gene            4329..7862
                     /gene="smc"
                     /locus_tag="AC1PNG_000703"
     CDS             4329..7862
                     /gene="smc"
                     /locus_tag="AC1PNG_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIP6701223.1"
                     /translation="MYLKSIEMQGFKSFADKTKVVFDRGVTAVVGPNGSGKSNITESL
                     RWALGESSAKSLRGGKMPDVIFSGTESRKALNYASVVVTLDNSTGFIANKQKEIKVER
                     HIYRSGDSEYLIDGQKVRLRDIHDLFMDTGLGRDSFSIISQGRVEAIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETESKLAQAQGNLDRLDDIIYELDNQVKPLEKQAQTAKKFLEL
                     DGQRKELYLDVLVAQLLLGKEKLSEKEAELESVKTELTSYYKQRSELEQENQNLKEKR
                     HRLSEQLEREQAVLLDLTKLISDLERKIEVHKLESSQNESSRQEAQARLENLLTRREQ
                     LAEQIEQKQETLAQLDSSLSSLKDDIAAVDKEISYFSEDPDQVLDHLREQYVALMQEE
                     AEASNSLTKIQQDIANQISLSESKSEDLARLQTEKQTAQEVLDKSRKSLEEADHVLRQ
                     LLESYQIKKSELDQTQTAYQAEQGRLFDLLDQLKGKQARQSSLEAILKNHSNFYAGVK
                     AVLQAAPSLGGIIGAVSEQLTFDTRYQTALEIALGGASQNVIVEDEATAKRAIAFLKE
                     KRQGRATFLPLTTIKPRQLSGQQVSLLESSEGFLGLASDLVTYQPNLDAIFQNLLGTI
                     AIFDSIDHANQAARATKFQVRMVTMDGAEIRPGGAFAGGSNRINSTTFIKPELDALLG
                     EIAELSSQLQEQESLVAAKKTSLDQTREALETIKAEGEEARLNQQSARIHQEQAENRL
                     AQLSAQYDLQMSQVSPTILTELEEAAAKEEVNVQALNEKKLALDQQINQVRDNRDSIQ
                     ESLQKLQTQKGQLTLEQAELTSQLRFEQTDLRRLQEEKVVADKEISLLEDMIDQKLEA
                     LEDTSIEILEEQLQAASDKQNQTNQILIRLKFELEDIDGQFEDLEERLQQARTKNDDL
                     IRKQAKLEADCEQAGDKLRTLLGNLTEHFKLSFEAAQSQAKEVENLAVAEQSLKDLER
                     AIRALGPVNLDAIEQYDEVNNRLTFLNEQRTDILSARDLLLDTIHEMDDEVKERFKVT
                     FEAIRESFKQTFKQMFGGGSADLILTEGNLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKESQFIVVTHRKGT
                     MAAADAMYGVTMQESGVSKIVSVKLKDVEKL"
     gene            7859..8668
                     /locus_tag="AC1PNG_000704"
     CDS             7859..8668
                     /locus_tag="AC1PNG_000704"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIP6701224.1"
                     /translation="MINKIYASDMDGTFLREDHSFDKERFRRILNQFKEKGYLFVATS
                     GRSMQSLKLVFEDFVDEIGFVAENGSIVEYQGQTIFMDDPISPEIYLPIIAGIDAGPF
                     GSRRSMVLSGLENFYLLKNAEPQFLEAMTNYYAHFRLVDTFEEVREEIIKINAKFSPE
                     EMDDARRWLNDTFEGVTAMTTGFDNIDIIPNGSNKSVGLSHLCAHFGITRQDVVAFGD
                     NQNDLDMFDFAGLALATENAREEVKAQADWMIGHCNDGAVLAYLEEEVNGN"
     gene            8658..9470
                     /locus_tag="AC1PNG_000705"
     CDS             8658..9470
                     /locus_tag="AC1PNG_000705"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIP6701225.1"
                     /translation="MAIKLIALDLDGTLLTSDKRISEANKKALQAARERGVYVVLTTG
                     RPLQAIGTFLEELDLLGENQYSITFNGGLVQENTGRVLDKVGFTIEDVRTIRRVTNDL
                     DLPLDVLYGGDVYSLPSAHESLYLTCNPLLNKIVASDEEMPEDFVYNKAVSAVAADFL
                     DGQIPKIPAELHERFEIFKSRDILLEWSPKGVHKANGLAKLIGHLGIDQSEVMACGDE
                     GNDLSMIEWAGLGVAMANATDEIKSAANLVLPKNNDEDGIAWVIEHYVLNED"
     gene            9472..10746
                     /gene="ftsY"
                     /locus_tag="AC1PNG_000706"
     CDS             9472..10746
                     /gene="ftsY"
                     /locus_tag="AC1PNG_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIP6701226.1"
                     /translation="MGLFDRLFGQKKQEEQVALVEEVEREQSASSEDTVTTEVESATQ
                     EMEKFQSEVAETELTAEQEAQKQRTLDMMAQYYAAKEAAAARVKEAQEQGFDPAIQTK
                     VKEEPVEEVAPVGQESEQDKYQRTLKKTRTGFGARLNEFFANFRSVDEEFFEELEEML
                     ILSDVGVQVASTLTEELRYEAKLQNAKKTDELRRVIIEKLVDIYEKDGQFSERINFQD
                     DLTVMLFVGVNGVGKTTSIGKLAYKYKQAGKKVMLVAADTFRAGAVAQLVEWGRRVDV
                     PVVTGPEKSDPASVVFDGVKRAVAEGVDILMIDTAGRLQNKENLMAELEKIGRIIKRT
                     LPDAPHETLLALDASTGQNALSQAKEFAKITPLTGLVLTKLDGTAKGGVVLAIRQELD
                     IPVKLIGFGEKIDDIGEFKSEEFMRGLLEGLV"
     gene            complement(10770..11327)
                     /locus_tag="AC1PNG_000707"
     CDS             complement(10770..11327)
                     /locus_tag="AC1PNG_000707"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6701227.1"
                     /translation="MQHKGTQILETERLILRPFQASDVESVFQNWTSDEKVTTYLTWP
                     THQTLQDSEDYVQFCLQSYSQEKTYRWVIELKENQQPIGDISVVSLDERVQAAELGWV
                     LGSKWWGQSYMAEALEAVNHYLLEEVGCLRITAVHDSENRPSGRVMEKVGMTYEGTLR
                     QAARNNRGIVDIAIYSLLHTDRKSR"
     gene            complement(11329..12003)
                     /locus_tag="AC1PNG_000708"
     CDS             complement(11329..12003)
                     /locus_tag="AC1PNG_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIP6701228.1"
                     /translation="MTELNTILTQLQAASHAGTLKRYEKIGETKPYYGVPMGAISGIA
                     KAYKNRLDLFAPLWQTGVLEAQYLAIQIAKNKPDQLPQADLENCLNEQISVNVLDKLA
                     SIILSKRKDSRVWEEDLLAKDQAIFQRLGWFLRAKYFAGKTAKNQEIEETLDHIRQHL
                     QTADPLIQWTMNQCLVEIAVAYPDYLEQGLVIGQELAVYSDMKVPKGCTSAYAPDWIE
                     ALLRRK"
     gene            complement(12145..13632)
                     /gene="zwf"
                     /locus_tag="AC1PNG_000709"
     CDS             complement(12145..13632)
                     /gene="zwf"
                     /locus_tag="AC1PNG_000709"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIP6701229.1"
                     /translation="MSSHVLFTIFGASGDLAKRKLYPSLFRLYKAGHIKENFAVIGTA
                     RRPWTKEFFEQTVIESLGDLPDTPRQAHEFASHFYYQSHDVNDTEHYVALRKLQDDLC
                     EKYNTQHNKVFFLSMAPEFFGTIAKHLKSEQIVDGQGFERLIIEKPFGTSLETASKLN
                     DELAAAFNEDQIYRIDHYLGKEMVQNIFAVRFANIIFEHIWNRDYIDNVQITFAEAIG
                     VEDRGGYYDHSGALKDMVQNHALQVLSLLAMDKPASFKEEDVRAEKIKVFQHLRQPSD
                     EDLKRNFIRGQYAAGSIDGKDYVSYLDEPNIAEGSQTETFAGGVFFVDTDRFRDVPFF
                     FRTGKRLTEKGTRVTITFKHAEDIFGQPSEANVLTIFIQPTEGFMLSINGKEVGSHFA
                     LTPAKLNFRHNATALGNSPEAYEKLFFDVLNGDSTNFSHWEEVKASWSLIDRIVDLWA
                     SNQVPLHTYPAGTMGPEATFDLLESYGCKWVWTPDVWYRERGLLK"
     gene            complement(13711..14526)
                     /locus_tag="AC1PNG_000710"
     CDS             complement(13711..14526)
                     /locus_tag="AC1PNG_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family putative permease subunit"
                     /protein_id="HIP6701230.1"
                     /translation="MIRFLILAGYFEMTMYLYISGKLDQYINLHYSYLAYLSMVLSFI
                     LAIVQLYVWVKEIRVHSHLKSKIAKLSSVGLLLIPLAIAWLFPTVSLDSTTVAAKGYH
                     FPLAAENDTNTQNQEGTTVQYLKPDTSIYFTKSAYQNQMREIADRYLAEETIIVTNEN
                     YMEVMEAIYDYPTEFSGKTIEMIGFVYNDPDNSQQFFLFRFGIIHCIADSGVYGLLST
                     GATTEFPNNTWVKARGTIKVSYHTSLKQNLPTLELQSMVQIEQPDSPYVYRVF"
     gene            complement(14523..15428)
                     /locus_tag="AC1PNG_000711"
     CDS             complement(14523..15428)
                     /locus_tag="AC1PNG_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HIP6701231.1"
                     /translation="MFGLDQLPTSVLQAGAIFLSIMIEALPFVLIGAIISGFIDVFIT
                     PDKVRKLLPKNKFLAILFGTFIGFIFPSCECGIVPIVNRLLEKKVPSYTAVPFLVTAP
                     VINPIVLFATFTAFGNSLLFAFYRALGAIIVSLVLGIILGFFVKGPILKGSKFDHHQH
                     GYSDKTSWQKVGFALIHAIDEFFDTGRYLVFGCLFASLVQVYIPTAVLTTIGHSPLTA
                     ILLMMLLAFLLSLCSEADAFIGASLLSSFGFAPVMAFLVIGPMVDVKNLLMMKHYFKG
                     KFIVGFIITITLVILGYSLILGGVV"
     gene            complement(15430..>15574)
                     /locus_tag="AC1PNG_000712"
     CDS             complement(15430..>15574)
                     /locus_tag="AC1PNG_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HIP6701232.1"
                     /translation="AVTYKRQDDLEKMLEEFASFEKLEEIEYPDPKSKENSEKNNLNQ
                     DKK"
BASE COUNT         4526 a         2900 c         3777 g         4371 t
ORIGIN      
        1 gtttatgaaa aagttgattc acaataaatt atttctatct agctttgtag cagacttgat
       61 ttctaacttt ggagatactc tttactatct ggctttgatg aaccatgttt tactcttagc
      121 agataccaag tttgccctgg ctatgattac cgcatctgaa accttgccga ttttggctgg
      181 tctctttatc gggatttggg cagacaagac caaaaacaaa ttggatacca ttcttgcaac
      241 tctggtggtt cgtgtcggtc tctatgcctt tgttggcttc ttgatgggat ttgcaccagc
      301 cctctgggtt gtggcggtgg tctgcgtcat caacttcctg tctgaccttg ctggtcaata
      361 cgaaaacggt ctctatatgc cagtcagctt gcgggttgtg gctgctgaag accgtgaaac
      421 agctatggct tttaagatga cggtgaaaag tcttttgcaa attgccttcc aagctagcgg
      481 tgctatcctc atcggattca tgtcttacca gaatctcgcc ttcttcaatg cagggacctt
      541 cttggtcagt cttgccatca tggcaaccct ccgtccagct cttgccaagc tactgaagga
      601 aaatcctatc caagaagctg agcaaacaga aacagaagca ggacttatca agggtatgcg
      661 taacagtctc aaagaatcct accaagctgt tcagaatatt cttgttctca aagcgtcaat
      721 tgtgaccatt gccagcctga atgccatttt tacagcactt tcaccattag ttattctcaa
      781 tatgaaggag ttctcagact ttgtgattgt caatgcagga acaaccgttg ctttgatttc
      841 cattttcttc tcagttggta gtatcttggg gtcaagtatc ggtatggctt tgttcaaaaa
      901 tgttagcttg atcaatctcc tcaaattctc tacattgatg ccagtcttga ttttctcggg
      961 tttctttctc cacaatattt atgtggtatt gacggttatt tttgtgacag ctatgacttt
     1021 gggtgtcttc aatccgaaaa tgaatgcctt ggtcttaaat gaactgcctg aagataaatt
     1081 ggcgacagtc ggtggaggga ttgatgcttt ctgccagatt ggtatggtag caggtcaagc
     1141 cttggtagcc cttatggtaa caatcttatc tgtcacaagt atttccctca tcttcctctt
     1201 gctttctgtg ggactagtaa gctatactat tcttgtgggt tgtaaaccta aaaatgaagt
     1261 agctcaagtt tagaaagtat gcgtatgaaa ttagattatc gagattaccg cagtgaaatt
     1321 gtggaaaaac tgttcattcc cttgattgtc aaggagcgtg aggagccgtt tgaagcggat
     1381 ttgtcacaga aggagaaaga cattctcaaa gatgccttgg acattcgcaa tgaagtagaa
     1441 aacaagctgg cggattttcg tcaggaagtc aatgaggtct ttgtttgggg ccatatcttt
     1501 acaatcctgc acaccttgta tttctacctc ttggatcaag gacaggatcc aaagacagtc
     1561 gaggaggctt gtcagctgat tttgacctta tcgcaagaag aggtagaaga tgctatgcgg
     1621 accatgctag cgtctgaaaa tgacggtcat cgtgaaaagg acctcagcct catggaactc
     1681 ttggaaaaga cagataaaaa gccagcagaa aagtggtatt ggtcactagc ccttcgcaat
     1741 cccttggaaa ctgttgagcg gtctgtcgat ctcctgaaca agctactccc aatctatcag
     1801 ccgtattttg aaggggcaag agcagagcgg gaagcctttg ctagagactt tgacattgaa
     1861 caactctaca gagaatccaa gcagttagcc atgaccagct tggatagctt gggtgtagaa
     1921 actgcccaat tcttcgttct cagtccatgg aattattggt ttgcttatta tggcaatgaa
     1981 caatttgact atatgaaagt tgctctttta gcttcctgcc gaattgatca gattatgctg
     2041 tcaaatgacg agttggatct ggatgatttg acaacggctc ttaaagtcat cagcgatagt
     2101 acgcgttatc aggtcttggt ggagctgacc aagcctcatg ccaagagcaa ggacattgca
     2161 gaacgcatgg ctattacagg ggcggccgta tccttccaca cccaaaagct aattaacggt
     2221 gacttacttc tcttcaatac aaaaaatagc gatgtcaaat acagtgtcaa tcgtgaactt
     2281 ctccaagagg tcattgccaa attgacagag gattttgatt tgtaaaaaaa gccttgtaag
     2341 ttagatctta caaggttctt ttgtgcttat actcttcgaa aatcgaaatc agacgttgtt
     2401 gacttgattt gatgaacttc agttctatcc gcatctgcgt cgcctagtct gtttttgatt
     2461 ttcattgagt attaatgatt aaccagctga gtagttggca agtgatgatg gtgataagga
     2521 agactaacca cccttgctgt attttggtca gggaactagt ttctaggaag aaaaagctga
     2581 tttcgactgg gataacatgg ataaaataat aatagctggc gtattctttt agaaattgga
     2641 attttttctt gcggaagagg tctgtggtta aggtccaagc gactagatag gctgtgaacg
     2701 gaatcaggct aaatagaaag ttcttgtcgt agccctgatt gagatagatg acagtggctt
     2761 caaaagctaa aagggctgtg cagacaagta gtttggtttt ttgtcggtgt tggaaaatgg
     2821 ggtgattgag tttgttggct agataaaagc ctatcaggac aaaaatcggt gcgtagaaga
     2881 ggccgttgcg actggtgaaa aagaaggttt tgtagttgtc aaatgcggtc aggaaaatcg
     2941 attctgctag gtaggcggag taggtttcga ctgagcctag aagatagaga aggcaggcta
     3001 gacaagctgt gatgcgccag ccccatttct ccaaggaaag atggacgagg ataagaccta
     3061 gaaagaaagc gggcatgtac cagagttggt agctggtgcc ggtataggtc agtccgacta
     3121 ggagtgcgag gggatagtag aggaagggga tttcttggag tgttaggtag atgaggtaga
     3181 agggcaggta gaggatggac caggtcaggt attgtttgct gtagcggatg aggtattgtt
     3241 tggagaggct ggggtagttg cgtcggagga agaagctggc ggtgaccatg aagtagggaa
     3301 cgaccatgcg gctaaagatt gatttttgga tgaagtggag ctggtctgct tcaaatagtc
     3361 ttcgacagtg aagggcaacg acgaggatgg ctccgatgaa ctggatgagg gagagcagtg
     3421 atgtattgag ttgttttgtc ttattttgca tgctaacgtt cttctttttt cttctatttt
     3481 atcgaaatct gatgaatatg taaatgtttg caaaaagatg tggagaggtg ctggctgaaa
     3541 cttagttgac aagagaagtc cagtggaaaa tatggtataa tgttaggacg gagtaaatgt
     3601 aaaccatcta tttcgtttaa gttagaaagg agcatataga tgaaagattt acatgcaaaa
     3661 ttattggctg attttgggat tgatttttcg gatttgaatt tattagaaac ggcttttacc
     3721 catacttctt atgccaatga gcatcgcctt ctaaaaattt cacataatga gcgcttggaa
     3781 tttttaggag acgctgttct ccaattaatg atttccaaat atctctatca aaaatatcct
     3841 gatagacccg aaggtgagat gtccaagttg cgttcgacct ttgtacgtga ggagtctttg
     3901 gctggttttt cacgtgcttg tggttttgat cggtttcttc gtcttggtaa gggggaagag
     3961 aagtctggag ggcgaaatcg cgatactatt ttaggagatg ctttcgaggc gttcttagga
     4021 gctctgctat tggacaaagg ggaaaagacc gtggaggaat ttatccataa ggtcatgatt
     4081 cctcgtttag aaaaagggaa ttttgagcgc gtgaccgact ataagactgc tttgcaggaa
     4141 attttgcagg tcaatggtga gattgctatt tcgtatcaag taatagctga gtctggacct
     4201 gcccatgata agacctttga agtggaggta tctgctgatc ggcgtgtgat tggtcgtggt
     4261 cgtggtcgct ctaagaaatt agctgaacaa gccgccgcca aaaatgctgt ggaggctaga
     4321 ggataggtat gtatctaaaa tcaattgaaa tgcagggctt taagtccttt gcggataaga
     4381 ctaaggtagt ctttgatcgt ggagtgactg ctgttgtagg gccgaatggt tctggcaagt
     4441 ccaatattac agaaagtcta cgttgggcct tgggagagtc gtctgctaaa agcctacgtg
     4501 gaggcaagat gccagacgtc atcttttcgg gaacagaaag ccgcaaggct cttaactatg
     4561 cctcggttgt cgtgaccttg gataatagca caggttttat cgccaataaa cagaaagaaa
     4621 tcaaggtaga acgccacatt tatcgatctg gtgatagtga gtacttgatt gatgggcaaa
     4681 aggttcgtct acgggatatt catgatcttt tcatggatac gggcttggga cgtgattctt
     4741 tctctatcat ttcacaaggg cgggttgaag caattttcaa ttccaagcct gaggaacgcc
     4801 gtgctatttt tgaagaggcg gcaggggtct taaaatacaa gacgcgtaaa aaagaaacag
     4861 aaagtaagtt ggcacaggcc cagggcaact tggaccgctt ggatgatatt atctatgaat
     4921 tggataatca ggtcaaacca ttagagaaac aggcacaaac tgctaagaaa tttctagaat
     4981 tagatggtca acgtaaggaa ttatacttag atgttttggt ggctcagttg ttgcttggta
     5041 aggaaaagtt atcggaaaaa gaagccgaac tagagtctgt caaaactgaa ttgactagct
     5101 actacaaaca gcgttctgag ctggagcaag aaaatcagaa cttgaaagag aagcgccacc
     5161 gcctttcaga acaattggag cgtgaacagg cagttctgtt ggatttgacc aagttaatca
     5221 gtgatttgga gcgaaaaatt gaggtccata aactggaatc tagtcaaaac gaatcaagtc
     5281 gtcaagaagc gcaggcgcgc ttggaaaact tgttgactag acgagagcag ttggcagaac
     5341 agattgagca aaagcaagaa acgcttgcgc aattagatag ttccttgtct agcttgaaag
     5401 acgatattgc tgcggtagac aaagaaattt cctacttttc agaagatcct gatcaggttt
     5461 tagatcatct gcgtgaacag tatgttgctc tgatgcagga agaggcagaa gcttcaaaca
     5521 gtctgaccaa gattcagcag gatattgcca atcaaatcag tctttctgag agtaaatctg
     5581 aagacttggc acgcttgcag acggaaaaac aaacagctca agaagtactt gataagagtc
     5641 ggaaaagttt agaagaagca gatcacgttt tacgccagtt gcttgagagc tatcagatta
     5701 aaaaatcgga attagatcaa acccagactg cttatcaggc tgagcaaggt cgcttgtttg
     5761 atttgctgga tcagttgaaa ggcaagcagg ctcgccagtc tagtttagaa gctattttaa
     5821 aaaatcactc caatttctac gcgggggtta aggctgtctt acaggcggca ccaagcttag
     5881 gaggaattat tggagcagtc agtgaacaat tgacctttga tactcgttac caaacggctt
     5941 tggaaattgc tcttggcggt gctagtcaaa acgtcatcgt tgaagatgaa gcgactgcta
     6001 aacgagctat cgcatttttg aaagaaaaac ggcaaggacg ggcaaccttc ttacctctaa
     6061 caactatcaa gccacgtcag ctttctggac agcaagtctc tttactagaa tcttcagaag
     6121 gattcttggg gcttgcaagt gatttggtga cttatcagcc aaatttagat gcgattttcc
     6181 aaaatttgct aggcactatt gcgatttttg atagcattga ccatgccaac caggcggcgc
     6241 gtgcgaccaa gtttcaagtg cgtatggtga ccatggatgg agcggaaatc cgtcctggtg
     6301 gagcctttgc tggtggtagc aatcgaatta acagcacgac ctttatcaaa ccagaactgg
     6361 atgctctttt aggagaaata gcagagctgt ctagccagtt gcaggagcaa gaaagcttgg
     6421 ttgcagctaa gaaaactagc ttagaccaaa ccagagaagc cttggaaacg attaaggcgg
     6481 agggggaaga agctagattg aaccagcaga gcgcaaggat tcatcaggaa caggcagaaa
     6541 atagactggc tcagctatca gcccagtatg acctgcaaat gagtcaggtt agtccgacca
     6601 ttttgactga gctagaagag gctgctgcta aggaagaagt aaatgtccaa gccttgaatg
     6661 agaaaaaact agccttggat caacaaatca atcaagtccg tgataatcgc gacagtattc
     6721 aagaaagctt gcagaaacta cagactcaga agggtcaact aactttggaa caggctgaac
     6781 tgactagcca acttcgtttt gaacagacag atctgagacg tttgcaagaa gaaaaagtgg
     6841 ttgcagataa ggaaatttct cttttagagg atatgattga ccagaagcta gaagctcttg
     6901 aagatactag cattgagatt cttgaagaac aattgcaggc cgcatctgat aagcaaaacc
     6961 agaccaatca gattctaatt cgcttaaagt ttgagttgga agatattgat gggcagtttg
     7021 aagacttgga agaaagattg caacaagctc ggactaagaa tgatgatttg atccgtaagc
     7081 aggctaagtt agaggcggat tgtgagcaag cgggcgataa attacggact ttacttggta
     7141 atttgaccga gcatttcaaa ttgagttttg aagctgctca gtctcaggcc aaggaagttg
     7201 agaatcttgc agttgctgaa caaagcctca aagacctaga aagagctatt cgtgctttgg
     7261 gtccagtcaa tttggatgct attgagcagt atgacgaggt caataaccgt ctgaccttcc
     7321 tcaatgagca acggacagat attttgtcgg cgcgtgattt actactagat actatccacg
     7381 agatggatga tgaagtcaaa gagcgcttca aggttacttt tgaagctatt cgtgaaagct
     7441 ttaagcagac attcaaacaa atgtttggcg gtggttcagc ggatttgatt ttgacagaag
     7501 gtaacctatt gactgcgggt gtggagattt ctgttcaacc tccgggtaag aaaattcaat
     7561 ctctcaatct tatgtcaggt ggcgaaaaag ccctttcggc cctcgctctg ctattttcca
     7621 ttatccgtgt taagaccatt ccttttgtaa ttctcgatga ggtggaggca gccttggatg
     7681 aggcgaatgt caaacgtttt ggcgactacc tcaaccgttt tgataaggag agtcagttta
     7741 tcgtcgtgac ccaccgtaaa gggaccatgg cggcagcgga tgccatgtat ggggtgacca
     7801 tgcaggaatc tggcgtgtcc aagattgttt ccgttaagct gaaagatgtg gagaaattat
     7861 gattaacaaa atttacgcca gtgatatgga tggtaccttt ttacgagaag accatagttt
     7921 tgataaggaa cggtttcgtc gtatactgaa tcagtttaaa gaaaaaggtt atttatttgt
     7981 ggcaacaagt gggcgatcta tgcaaagcct gaaacttgtt tttgaagatt ttgtagatga
     8041 aatcggtttt gtggcagaaa atggttcaat cgttgaatat caggggcaga ctatctttat
     8101 ggatgatcca attagtcctg aaatttatct gccaattatc gcgggtattg atgcaggtcc
     8161 ctttggtagc agacgttcca tggtgctgtc tggacttgaa aacttttatt tattaaaaaa
     8221 tgctgagcct cagtttttag aagcgatgac gaattattac gcacattttc gattggtaga
     8281 cacatttgaa gaagttcgag aagagattat aaaaatcaat gccaaatttt ctcctgagga
     8341 aatggacgat gctagacgat ggttaaatga tacatttgaa ggtgtaacgg ccatgacaac
     8401 tggttttgac aatattgata taattccaaa tggttcaaat aaatcagttg gattgagtca
     8461 tctctgtgct cattttggaa ttacccgtca ggatgtagtg gcatttggtg ataatcaaaa
     8521 tgatttggat atgtttgact ttgcaggtct ggctcttgcg acagaaaatg caagagagga
     8581 agtgaaggcc caggcagatt ggatgattgg tcattgcaat gatggagcag tattagccta
     8641 tttagaggag gaagtaaatg gcaattaagt tgattgcatt ggatttagat ggaacacttc
     8701 tgacatcgga caaacggatt tcagaagcta ataaaaaggc cttgcaggca gcgcgtgagc
     8761 gtggagttta tgtagttctg acaacagggc gtcctttaca agctatcggt acttttctag
     8821 aagaactaga tctgctaggg gagaatcaat attcgattac atttaacggt ggtctagtcc
     8881 aggaaaatac aggtcgggtt ttggataagg ttggcttcac cattgaagat gtgcgcacca
     8941 tccgtcgtgt gacaaatgat ttggacctgc ctttggatgt cctgtatggt ggcgatgtct
     9001 attctctacc atcagcccat gaatcacttt atctaacttg taatccattg cttaataaaa
     9061 ttgtggcaag cgatgaggaa atgccagaag attttgtcta caataaggca gtttcagctg
     9121 tggcagcaga ctttttggat ggacaaattc caaaaattcc tgcggaactt cacgagcgtt
     9181 tcgagatttt caaatcgcgt gatattctgc tcgaatggag tccaaaaggt gttcataaag
     9241 caaatggttt ggctaagttg attggacatc tggggattga tcagtcagaa gttatggcct
     9301 gtggcgatga aggaaatgat ctttcgatga ttgaatgggc tggtctgggt gttgccatgg
     9361 ctaatgcgac tgatgaaatc aagtctgcgg ccaaccttgt cctgccaaaa aacaatgatg
     9421 aagatggcat tgcttgggtc atcgagcact atgtattgaa tgaggactag tatgggatta
     9481 tttgatcgat tatttggaca aaaaaaacaa gaggagcaag tcgctcttgt tgaagaagtg
     9541 gaaagggagc agtcggcttc tagcgaagat acagtaacta ctgaagttga gtcagctact
     9601 caagaaatgg aaaagttcca gtctgaagta gcagagactg aattgacagc cgaacaagaa
     9661 gctcagaaac aacggacctt agacatgatg gctcaatatt atgcagccaa agaagccgct
     9721 gcggctcgag tgaaggaagc gcaggaacaa ggctttgacc ctgctattca aaccaaggtc
     9781 aaggaagagc ctgttgagga agttgctcca gtagggcaag aaagtgaaca ggacaagtac
     9841 cagcgaacct tgaaaaaaac tcgtacagga tttggggcaa ggctcaatga attctttgcc
     9901 aatttccgtt cggttgatga agaatttttc gaagagttgg aagaaatgct gattttatca
     9961 gacgtaggtg tgcaagttgc ttctacgctg acagaggagc tccgctatga agccaagcta
    10021 caaaatgcca agaagacaga tgaactccgt cgggttatca tcgaaaaatt ggtggatatt
    10081 tacgaaaaag atggtcaatt cagcgagcga atcaatttcc aagatgattt gacagtcatg
    10141 ctctttgtcg gtgtcaacgg tgttgggaag acgacgtcta tcggtaagtt agcctacaag
    10201 tacaagcaag ctggcaagaa ggtcatgttg gtcgcagcgg acaccttccg tgcgggtgcg
    10261 gtagcccagc tggtcgagtg gggtcgccgt gtcgatgtgc cagtagtcac aggacctgaa
    10321 aagtcagacc cagctagtgt ggtttttgat ggggtaaaac gtgccgtggc agaaggcgtg
    10381 gacattctca tgattgacac agcaggtcgc ttgcaaaata aagaaaacct tatggcagag
    10441 ctggaaaaaa tcggtcgcat catcaaacga accctacctg atgcaccgca tgaaaccctc
    10501 ttagcactag atgcctccac aggtcaaaac gccctcagtc aagccaagga atttgcaaaa
    10561 attactccat tgactgggct agtcttgact aagctagacg gtacagcaaa aggtggtgtt
    10621 gtccttgcca tccgtcagga actggatatt ccagtcaagc tgattggttt cggtgaaaaa
    10681 attgatgaca taggtgaatt caaatccgaa gaattcatgc gtgggctttt ggaaggattg
    10741 gtatagaaaa aagtcgttag ttcaatgaac tagcgacttt ttctatctgt atgcaacagt
    10801 gagtaaatgg caatatccac aataccccga ttatttcgag cggcttggcg gagagttcct
    10861 tcataggtca taccgacttt ttccatgaca cgaccagaag gacgattttc actatcgtga
    10921 acagctgtaa tccgtaaaca gcccacttcc tccaataaat agtgattaac agcttcaaga
    10981 gcttcggcca tataactttg cccccaccat ttactgccca acacccaacc taattcggca
    11041 gcttgaactc tttcatcaag gctgacaacg gaaatatctc caatgggttg ctgattttct
    11101 ttgagctcaa ttacccacct gtatgttttt tcctgtgaat aggattgaag acagaactgg
    11161 acatagtctt cactatcttg gagtgtctga tgagttggcc aggtcagata ggtggtaacc
    11221 ttttcatctg aagtccagtt ttggaaaact gattcaacat cgcttgcttg aaaagggcgt
    11281 aaaattagac gttctgtttc taaaatctga gttcctttat gttgcatctt atttcctcct
    11341 caacaatgcc tcaatccagt ctggtgcata ggcagaagta cagccttttg gcaccttcat
    11401 atcggaatag acagcgagtt cctgaccgat tacaagtccc tgttccaggt agtcgggata
    11461 ggcaacggca atctccacca agcattggtt catggtccat tggataagcg ggtctgcggt
    11521 ttgtaaatgc tggcgaatat ggtctagagt ttcttcaatt tcttggtttt ttgctgtttt
    11581 tccagcaaag tatttggcac gaaggaacca acctaggcgt tgaaaaatag cttggtcttt
    11641 ggcaagcaaa tcttcttccc aaaccctgct gtcttttcgt ttgctgagaa tgatggaagc
    11701 cagcttatcg agcacattga ccgaaatttg ttcattgagg caattctcta ggtcagcttg
    11761 gggaagctgg tcaggcttat tttttgcaat ttgaatagct aaatattgtg cttcaaggac
    11821 acctgtttgc cagagtgggg caaataagtc cagtcgattt ttataggcct tggcgatacc
    11881 agaaatagct cccattggaa cgccgtagta gggcttagtt tcgccaattt tttcataacg
    11941 cttgagggtg ccagcatggc tggctgcttg gagttgtgtg agaatggtgt ttaattcagt
    12001 cataaatatc tcccaaaaaa actggattgc tccagttttt atatgtagtc gtttagtttg
    12061 cttttagtac aaggcaacga gctgtaggct gtactaaagt acggcaaagc gagttaacga
    12121 agtaatcaaa gataaactaa atgactattt caataagcca cgttcacgat accatacatc
    12181 tggtgtccaa acccacttgc aaccgtagct ttctagcaaa tcaaaggttg cctcaggtcc
    12241 catagttcca gctggatagg tgtggagtgg gacttggttg cttgcccaca aatcaacaat
    12301 gcggtcaatc aagctccagc ttgctttcac ttcttcccaa tggctaaagt tggtagagtc
    12361 gccgttcaag acatcgaaaa atagtttttc gtaggcttcg ggtgaatttc caagagcagt
    12421 tgcattgtgg cggaagttga gtttggcagg agtaagagca aagtgggaac caacttcctt
    12481 gccgttgata gagagcatga agccttctgt tggctggatg aagatggtca atacattggc
    12541 ctcagaaggc tgaccaaaaa tatcttccgc atgcttgaag gtgatggtca cgcgcgtgcc
    12601 cttttctgtc agacgtttac ctgtacggaa gaagaaaggt acatcacgga aacggtcagt
    12661 atcaacgaag aagacacctc ctgcaaaggt ttctgtctga gaaccttcgg caatatttgg
    12721 ttcatccaag tagctaacgt agtcttttcc gtcgatggaa cctgctgcat attgaccacg
    12781 gatgaagttg cgtttgaggt cctcatcaga aggctgacga aggtgttgga agaccttaat
    12841 cttttcagcg cggacatctt cctctttgaa gctggctggc ttatccattg ctagaaggga
    12901 taggacttga agggcatggt tttgcaccat gtcttttaag gcgccagaat gatcgtagta
    12961 gcccccacga tcctcaacac caatcgcttc agcaaatgta atttggacgt tatcgatgta
    13021 atcgcggttc caaatatgct caaaaataat attggcaaag cgaaccgcaa agatattttg
    13081 taccatttcc ttgcctaggt aatggtcgat acggtagatt tggtcttcat tgaaggcagc
    13141 ggctaattca tcattgagtt tgcttgcagt ttcaaggctt gttccgaaag gtttttcgat
    13201 aatcaaacgc tcaaaacctt gtccatcaac gatttgctca gatttgaggt gtttggcaat
    13261 ggttccgaaa aattcaggcg ccatagagag gaagaagacc ttgttgtgct gggtattgta
    13321 tttttcacac aagtcgtcct gcaatttacg aagagctaca taatgttccg tgtcattgac
    13381 atcgtggctt tggtagtaga aatgacttgc aaattcatga gcttgacgtg gtgtgtcagg
    13441 cagatcacct agtgattcaa tgacggtttg ctcaaaaaat tccttggtcc agggtctgcg
    13501 agctgtacca attactgcaa agttttcctt gatgtgtcca gccttataga ggcgaaataa
    13561 agatgggtaa agtttgcgtt tggcaaggtc gccactagca ccaaaaattg taaacaatac
    13621 atgtgatgac ataatttcat tcctatctgt tgaatatcat taatagtata ccacaaattg
    13681 tcagaaaatt cactttttga gttggaatta ttagaaaaca cggtagacat acggggaatc
    13741 tggctgttca atctgaacca tcgattgtaa ttcaagtgtt gggagatttt gtttcaacga
    13801 tgtgtggtag gaaaccttga ttgttcctct ggcttttacc caagtattgt taggaaattc
    13861 tgtggtagcc cccgtagaga gtaagccata taccccagaa tcagcaatgc agtgaataat
    13921 accaaagcgg aaaaggaaaa attgctggct attgtcaggg tcattgtaga caaatccaat
    13981 catttcaatc gtttttcctg aaaattccgt aggatagtcg tagattgctt ccatcacttc
    14041 catatagttt tcattagtaa cgataatggt ttcttcagcc aagtatctat cagcaatttc
    14101 acgcatctgg ttttgatagg cagattttgt aaaataaata gaagtatcag gcttgagata
    14161 ctgaacagtc gtcccttctt ggttttgtgt attagtatcg ttttctgctg ctagtgggaa
    14221 atgatagccc ttggcagcaa cagttgttga atccaggctt accgtaggaa agagccaggc
    14281 tatagcaaga ggaatgagca gtagtccaac gcttgatagt ttggcaattt tgctttttaa
    14341 atggctatgt actcgaattt cttttaccca gacatacaat tgtacgatgg caagtatgaa
    14401 agataaaacc atggatagat aggctagata gctatagtgc agattaatgt attggtctaa
    14461 ttttccagag atataaagat acatggtcat ttcaaagtaa cctgcaagaa ttaaaaaacg
    14521 aatcatacta cacctccaag tatcagacta tatcccaaaa taacgagagt gatagtaatg
    14581 ataaaaccga ctataaattt ccctttgaaa taatgtttca tcatgagcaa gtttttaaca
    14641 tccaccatgg gtccgataac taagaaagcc attactgggg cgaatccgaa gctggatagt
    14701 agtgatgcac caataaaggc atcggcttcg gaacataggg aaagaagaaa ggccagcaac
    14761 atcatcagta aaatagctgt taatggactg tgaccgatgg tagtcaggac agcggttgga
    14821 atatagactt gtacgagact ggcaaataga caaccaaaga ctaggtaacg tcccgtatca
    14881 aaaaattcat caatggcatg aataagtgcg aagccaactt tttgccaact ggttttatct
    14941 gaataaccat gctggtgatg gtcaaacttg cttcctttaa gaattggtcc ttttacgaaa
    15001 aaaccaagaa tgattcccaa tactagggat acaatgatgg ctcctagggc tctatagaag
    15061 gcgaaaagta gggaatttcc aaaagcggta aaggttgcaa aaaggacgat aggattaatc
    15121 acaggcgccg ttactaaaaa gggaacagca gtataactcg gcactttttt ctctaatagc
    15181 cgattgacaa tagggacaat accacattcg caagacggaa aaatgaagcc gataaaggtt
    15241 ccaaatagaa tggcaagaaa cttgttttta ggtaagagtt tacgaacctt atctggtgtt
    15301 ataaacacat cgataaaacc tgaaataata gctccaatca agacaaaggg caaggcttca
    15361 atcatgatag acaggaaaat agcccccgcc tgtaggacgg aggtaggtaa ttgatcaaga
    15421 ccaaacatat tattttttat cctgattcaa gttgtttttc tcggaatttt cttttgattt
    15481 tggatctgga tactcaattt cttccaattt ttcaaatgaa gcaaattctt ccagcatctt
    15541 ttctaaatca tcttgtcgtt tataagttac agcc
//
LOCUS       DBNFEG010000033        15255 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.033, whole genome shotgun sequence.
ACCESSION   DBNFEG010000033 DBNFEG010000000
VERSION     DBNFEG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.033"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            211..480
                     /gene="rpsO"
                     /locus_tag="AC1PNG_000713"
     CDS             211..480
                     /gene="rpsO"
                     /locus_tag="AC1PNG_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIP6701233.1"
                     /translation="MAISKEKKNEIMAQYARHEGDTGSVEVQVAVLTWEINHLNDHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRRTDVNRYRELIHSLGLRR"
     gene            495..620
                     /locus_tag="AC1PNG_000714"
     CDS             495..620
                     /locus_tag="AC1PNG_000714"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701234.1"
                     /translation="MSSDIGSFSYVPLWKCSARKKLGVLRLIEEYVIRSVVYIAG"
     gene            783..3002
                     /gene="pnp"
                     /locus_tag="AC1PNG_000715"
     CDS             783..3002
                     /gene="pnp"
                     /locus_tag="AC1PNG_000715"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIP6701235.1"
                     /translation="MSKQVFETVFAGKKLVVETGQVAKQANGAVVVRYGDSTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGWMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDPDASAPMAAMFGSSLALAISDIPFNGPIAGVQVGYVNGELI
                     INPDKAQMEESLLDLTVAGNKDAINMVESGAKELSEDIMLEALLKGHEAIQELLDFQN
                     QIVAAVGKEKADVELLQVDPELQAEIVAAYNDDLKKAVQVEEKLAREDATNAVRETVI
                     AAYEEKYAEHEEFDRIMRDVHEILELMEHAEVRRLITEDKVRPDGRRVDEIRPLDAEV
                     DFLPNVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLGDEYKKRFLHHYNFPQYSVGS
                     TGRYGAPGRREIGHGALGERALEQVLPSLEDFPYAIRLVAEVLESNGSSSQASITAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIDGITPQILEEALAQAKKARFEILDVIEATIPEVRPDLAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEDGLVAIFSPDRDAIERTKEIIAGLVREAKV
                     DEVFQAKVVRLEKFGAFVNLFDKTDALVHVSEMAWTRVNKPEDLVEIGDIVDVKVIKI
                     DDKGRIDASMKALLPKPEGYVEPEKRERSDRPRRHKDNKEKKDNNFGEFKFHKVEKK"
     gene            3056..3673
                     /gene="cysE"
                     /locus_tag="AC1PNG_000716"
     CDS             3056..3673
                     /gene="cysE"
                     /locus_tag="AC1PNG_000716"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HIP6701236.1"
                     /translation="MGWWKDTIEIVKEKDPAARTTLEVLLTYPGVKALAAHRLSHWMW
                     KKGFKLLARMHSQFWRFWTNIEIHPGADIASGVFIDHGAGLVIGETAIIESGVMLYHG
                     VTLGGTGKDTGKRHPTVRKGALVSAHAQVIGPIEIGENAKVGAAAVVLADVPADVTVV
                     GMPAKIVRVHGQKDEKVIHDMEDGREHYTTKLAELREASHRSSHL"
     gene            3688..4461
                     /locus_tag="AC1PNG_000717"
     CDS             3688..4461
                     /locus_tag="AC1PNG_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HIP6701237.1"
                     /translation="MLLEEFENTSAVIEPTDMAIRGGGEVCETMIFSFNGEIVDRVRQ
                     LPESREGGYLESINGRHPWYILERDGVRVAVMLAVIGAPMAVGHLEELKACGFENFIV
                     LGSCGVLDKSLVADKIILPSSALRDEGTSYHYAPASDEISYDPALLLTMEKALDQAGI
                     EHVRAKTWTTDAFYRETSAKVKRRLAAGAMVVDMEASAIMAWANFRQAKVYQFFYTAD
                     YVDYHKNEWDARREERTADSMTFFEVAMAIARKLESTSC"
     gene            4455..4640
                     /locus_tag="AC1PNG_000718"
     CDS             4455..4640
                     /locus_tag="AC1PNG_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701238.1"
                     /translation="MLKKLVRQNWPYVLTAIAGTIMFILKFSQGSWQVGMIWLAATAY
                     WLVKLYQKYQVLKNTQK"
     gene            4651..5994
                     /gene="cysS"
                     /locus_tag="AC1PNG_000719"
     CDS             4651..5994
                     /gene="cysS"
                     /locus_tag="AC1PNG_000719"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIP6701239.1"
                     /translation="MIKIYDTMTRSLRDFVPLEEGKVKMYVCGPTVYNYIHIGNARSV
                     VAFDTIRRYFEYRGYDVTYISNFTDVDDKIIKAANEAGMTTKELSDKFIAAFKEDVAK
                     LGVKPATKNPRVIDYMGEIIDFVQVLIDKGYAYEASGDVYFRVEKAENYARLANKTLS
                     DLEAGASGRVEGEGQLKEHPFDFALWKTAKPGEVSWESPWGAGRPGWHIECSVMATEI
                     LGATIDIHGGGADLEFPHHTNEIAQSECKTGQTFANYWMHNAMLNINDEKMSKSLGNF
                     LTAHDMLEQIDGQVLRFFLSTQHYRRPLNYTEKAISDAEINLKYLKNTYTQPVQNSVD
                     KSALELHLAAFEAAMDDDFNAANGITAIFDFAKWINSGNYDETVKEAFGKMLEVFGIV
                     FEEEVLDSEIEALIAERQAARANRDFATADRIRDELAAQGIKLLDTKDGVRWTRD"
     gene            5987..6385
                     /locus_tag="AC1PNG_000720"
     CDS             5987..6385
                     /locus_tag="AC1PNG_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HIP6701240.1"
                     /translation="MTKAVDVNLINGIALAFEGDAVYSMYVRRHLIFKGLTKPNKLHG
                     EANKYVSAKAQAGLISALLEAQLLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGF
                     EAVLGYLHMTEQTERLDELVGWCIDNIERG"
     gene            6387..7241
                     /locus_tag="AC1PNG_000721"
     CDS             6387..7241
                     /locus_tag="AC1PNG_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6701241.1"
                     /translation="MNEKELAGWFPQAKLSQEADAQSLALGDGRYLNLADADLTERER
                     YLLSLLSHPQEQKPSSPWQSYLHGQSSLPQAVEAIQFIHVHLWSQKDAQLDAWQTMMT
                     DLLPSQLAQVQLSSQDYLFIIEQSRWTDYQTILADTLSALEFDFGLRLTFLVGQVWSR
                     QLADRWSQLYQAEAALFAHWRAHYHQSTVLSFSQLFLWGQGRAGLEFGMIKASMLQLI
                     DQQDMQDIILALWTEGAVVTKTAQILYIHRNTLQYRLDKWQDLTGLQLKDLTDLSFCY
                     QVLLDRLF"
     gene            7351..8481
                     /locus_tag="AC1PNG_000722"
     CDS             7351..8481
                     /locus_tag="AC1PNG_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6701242.1"
                     /translation="MVQLNLKNIYKKYPNSEHYSVENFNLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGEAYIDGVLMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYSKDEIKKRVEEAAQILGLTEFLQRKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRTEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNEAGT
                     GTIGRIEQIGSPEELYNKPVNKFVASFIGSPAMNFFTVTLQEGVLVGDGFKVDLPEGR
                     RKHLEEKGYNGKSFTLGIRPEDIKASQLELDTYPSSIVEAEVVVSELLGAETMLYSKV
                     GNTEFVSRVDARDYHNPGDKVRLTFNLNKAHFFDDETDQAIV"
     gene            complement(8521..9489)
                     /locus_tag="AC1PNG_000723"
     CDS             complement(8521..9489)
                     /locus_tag="AC1PNG_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HIP6701243.1"
                     /translation="MSLSLRTIKLIFATVLAIYLATVLGLSYTTAAGIIAILSVLDTR
                     KSSFKMARNRLFSTLLALTIAVLTFALFGFGIWTLGIYLALYVPLAYRFNWEAGIAPS
                     TVLVTHLLLEQDISLIFLGNELALFLIGAGLALLFNLYMPSQEKKIQAYHDQVEDLLK
                     QILLRFEAFLLNGDGRNEAELITQLDQTLEQALKVVYLDRHNQLFQQTNYQVHYFEMR
                     AAQNKILRTMAGNINKCLLEGRENVILSSLFERAAQQLSRENSAKELLLDIELFHATF
                     RERPLPQTREEFETRATLFQLLHDMEAFIRLKVDFYEVYKDEKDPS"
     gene            complement(9564..9953)
                     /locus_tag="AC1PNG_000724"
     CDS             complement(9564..9953)
                     /locus_tag="AC1PNG_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HIP6701244.1"
                     /translation="MTLIFGIVAGLIAFAIGGLWYGLIFRDAWIKASGIDMAKVEADR
                     QAGKNGQKEMVISLVIEVLTAVVAIFFIKTLGVSPLHAAGGMGVIAVLASLKNYVFEQ
                     RPINLILINESYKLVCYLVVGILALFA"
     gene            10091..10819
                     /gene="rlmB"
                     /locus_tag="AC1PNG_000725"
     CDS             10091..10819
                     /gene="rlmB"
                     /locus_tag="AC1PNG_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIP6701245.1"
                     /translation="MEHNDIVYGVHAVVESLEANTGNKLYIQDDLRGKNVEKIKALAA
                     EKKVSISWTPKKTLSDMTEGAVHQGFVLRVSEFAYADLSVILEKAEQEDNPLILILDG
                     LTDPHNFGSILRTADATQVAGIIIPKHRAVGVTPVVAKTSTGAVAHVPIARVTNLSQT
                     LDKLKEAGFWVFGTDMNGTPSHKWNTAGKLALIIGNEGKGISSNIKKQVDEMITIPMK
                     GHVQSLNASVAAAVLMYEVFRKKI"
     gene            10820..11341
                     /locus_tag="AC1PNG_000726"
     CDS             10820..11341
                     /locus_tag="AC1PNG_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIP6701246.1"
                     /translation="MKEKVLIVDGYNMIAFWQATKQDFKKGDLDAARTTLLRTLSHYA
                     AFEQIEVICVFDAHHVPGLRQQYDEFKVSVIFTEEEETADSYIERLSAQLNSDRRKQV
                     SVATSDLNEQWVVFSQGALRVSARELEERTRIIKKDLDKFVDQVELYTPRLNPWSDGN
                     FQALKEMMEEMKE"
     gene            11338..12201
                     /locus_tag="AC1PNG_000727"
     CDS             11338..12201
                     /locus_tag="AC1PNG_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIP6701247.1"
                     /translation="MTFTIVTDSTSDLPISWVQENDVTVLGLTINLDGVTYETVGENR
                     LTSADLLEKMASGSQPTTSQVNVGQFEEVFEEAAKEGHEVLYLAFSAALSGTYQSAVI
                     AREMVVDQYPDAVITIVDTKAATIGEGYLVMKAVEARAAGKTLAETKAIVEDLVPRLR
                     TYLLVDDLNHLVRGGRLSKAAALIGGLVNIKPLLALNAEGKLEAIAKIRGRKKGIKEM
                     LNLTLDNLDHSTVMVAYTGDIEAAEGVKSSLLEDDRISDVLLTELGPVIATHTGTGVL
                     AILSIGTAMRA"
     gene            12665..13111
                     /gene="rplM"
                     /locus_tag="AC1PNG_000728"
     CDS             12665..13111
                     /gene="rplM"
                     /locus_tag="AC1PNG_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIP6701248.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKSISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            13131..13523
                     /gene="rpsI"
                     /locus_tag="AC1PNG_000729"
     CDS             13131..13523
                     /gene="rpsI"
                     /locus_tag="AC1PNG_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIP6701249.1"
                     /translation="MAQAQYTGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTQGSYDVFVNVNGGGYGGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(13632..14852)
                     /locus_tag="AC1PNG_000730"
     CDS             complement(13632..14852)
                     /locus_tag="AC1PNG_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIP6701250.1"
                     /translation="MTITKTKNGTYRLKVYIPTETRMPLGIVNNNYFDKRFKTRKEAR
                     QAEIDLLTKINQIENNEFTGIGKGDILFKDFYESIWWEAYKAGQTTSTTKPPSKSTVA
                     NTKTCFRKHILPMLGNYTVQFLNQNKQVILNLMAAKASEYANFKTLRSYVISIFDWAE
                     ELEYIESNKIAKTLRRIKSTKKIQLAEARRDEDLYLTQEQLQEWFSAFQDDLASGKIS
                     LKDYVLFYLTFFLGDRKSESYALQWKHIDFEKSQIQLLQALDRYGDVKSTKGNKKTIF
                     AISTDLLQLLQGWKSQQKQELAKFGIITNPEQFVFTYIDTKGNINKPLHADYLNNKMK
                     TIKKRHPKLTHATPHKLRHTGATLAKKAGMSLEAISEALTHSDTLTTKTYVNTSNIVP
                     LSAGHVAYQHLKNE"
     gene            complement(14864..15181)
                     /locus_tag="AC1PNG_000731"
     CDS             complement(14864..15181)
                     /locus_tag="AC1PNG_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6701251.1"
                     /translation="MQVILPDDQIKQIQILISSLITSEIEKQIHHRNIDNSYLNKKQA
                     CDYLGISNNTLDEWIRKGLPSIKIGKTIRFSKQAIDSWLLSQNYKYPHFCFIMVMIFS
                     GST"
BASE COUNT         4291 a         3173 c         3689 g         4102 t
ORIGIN      
        1 actattttga cggtacggct aatttgtaaa ggtgaatttg aaaaagaata gagaaagtct
       61 actcggttta ttggaagata atatcagttg acaaaagagt tattccttga tagaataaat
      121 gaggtccaaa aaggacaaat ctagcttttt cttggttaca ggtcgccaac ctgtcactcg
      181 gattaggctc aaaacaaagg agaacatatc atggcaatct caaaagagaa aaaaaatgaa
      241 atcatggcac aatatgctcg tcatgaaggc gacacaggtt cagttgaagt acaagtagca
      301 gtacttactt gggaaatcaa ccaccttaac gaccacatca aacaacacaa aaaagaccac
      361 gcaacttacc gtggtttgat gaaaaaaatc ggtcgccgtc gtaacttgtt ggcataccta
      421 cgccgcacag acgtaaaccg ttaccgtgaa ttgattcact cactcggact ccgtcgttaa
      481 tataggaaac tgccatgtct tcggacattg gcagtttttc ttatgttcca ttatggaagt
      541 gtagtgctcg gaaaaagctg ggtgttcttc gtttgattga agagtatgtg attcgttcgg
      601 ttgtttatat agcaggttag attttccctc aaattccttc ctcaattccc cacattttcc
      661 gtcaaatatg ataaaataag aagtatgcgt ggttttgtgc aaaagagagt gaaacaagct
      721 tgtttgattg tcttttgtag aaaatacgca ggaaaaattt cagaaaaaat aggagacaat
      781 tcatgtcaaa acaagtattt gaaacggttt ttgctggcaa gaaattggtc gttgaaacag
      841 gccaggttgc caaacaagcc aatggtgcgg ttgttgttcg ctatggcgac tcaacagtcc
      901 ttacagcggc agttatgtcc aagaaaatgg caacgggtga tttcttcccg ctccaagtca
      961 actatgaaga gaagatgtac gctgcaggga aattcccagg tggctggatg aagcgtgaag
     1021 gccgtccgtc aaccgatgcg accttgacag ctcgtttgat tgaccgtccg atccgtccaa
     1081 tgtttgcgga aggcttccgt aacgaagtcc aagtcatcaa cacagtcctt tcttacgatc
     1141 cagatgcctc agctccaatg gcagccatgt ttggttcttc attggcattg gcaatctcag
     1201 acatcccatt caacggccca atcgcaggtg tccaggttgg ttatgtcaat ggtgaactca
     1261 tcatcaaccc tgacaaggct caaatggaag agtctctcct tgacttgaca gtagctggta
     1321 acaaggatgc tatcaacatg gtagagtctg gtgccaaaga attgtcagaa gacatcatgt
     1381 tggaggcact cctcaaaggt catgaggcta ttcaagaact ccttgacttc caaaatcaaa
     1441 tcgtagcagc agtcggcaag gaaaaagcag atgtggaact tcttcaagtg gacccagaat
     1501 tgcaggctga gattgtcgca gcttacaatg acgatttgaa aaaagcggtg caggtagaag
     1561 aaaaattggc ccgtgaagat gcgaccaatg ccgtgcgtga gaccgtcatc gcagcctacg
     1621 aagaaaaata tgctgaacac gaagaatttg accgtatcat gcgtgatgtc catgaaatct
     1681 tggaactcat ggagcatgca gaggttcgtc gtttgattac agaagacaag gttcgtccag
     1741 acggtcgtcg tgtggatgaa attcgtccgc tggacgcaga agtggacttc ttgccaaacg
     1801 ttcatggctc aggtctcttt acccgtggtc aaactcaggc actttcagtc ttgaccttgg
     1861 ccccaatggg tgaaactcaa atcatcgacg gattgggcga cgagtacaag aaacgcttct
     1921 tgcaccatta caatttccca caatattcag ttggttcaac tggtcgctat ggtgcaccag
     1981 gtcgtcgtga aattggtcac ggtgctctcg gtgagcgtgc tcttgagcaa gtcttgccaa
     2041 gcttggaaga cttcccttac gctatccgct tggtagcaga agttttggaa tcaaacggtt
     2101 cttcatcaca ggcttctatc acagctggta cccttgccct tatggcaggt ggtgtgccaa
     2161 tcaaggcacc agttgcaggg attgccatgg gtctgatttc agacggtacc aactacaccg
     2221 tcttgaccga tattcaaggt cttgaggacc acttcggcga catggacttc aaggtggctg
     2281 gtactcgtga cggtatcaca gcccttcaaa tggacatcaa gattgacggt atcacaccgc
     2341 aaatcttgga agaagccttg gcacaggcta agaaggcacg ttttgaaatc ctcgatgtca
     2401 tcgaagcgac gatcccagaa gttcgtcctg acttggcacc aactgcaccg aagattgaca
     2461 ctatcaagat tgatgtagat aagatcaaga ttgttatcgg taagggtggc gaaaccatcg
     2521 ataagattat tgcagaaact ggcgtgaaaa tcgatattga cgaagacggt cttgtggcga
     2581 tcttctcacc agatcgcgat gcgattgagc ggaccaagga aatcatcgca ggtcttgttc
     2641 gtgaggcaaa agtggacgaa gtctttcagg caaaagttgt tcgtttggag aaattcggtg
     2701 cctttgttaa cctctttgac aagacagatg cccttgttca cgtttctgaa atggcttgga
     2761 ctcgtgtcaa taaaccagaa gacctagttg aaatcggcga tattgtcgat gttaaagtca
     2821 tcaagattga tgataaagga cgtattgacg cttctatgaa ggctcttctt ccaaaaccag
     2881 aaggttatgt ggagccagaa aaacgcgagc gttctgacag accacgccgt cataaagaca
     2941 acaaagagaa aaaagataac aactttggtg aattcaaatt ccacaaggtt gaaaagaaat
     3001 aagctgagta gacaagttcg gtagcgggct tgtcttctcg ctagaaggag tgactatggg
     3061 ttggtggaag gacaccattg agattgtaaa agaaaaagat ccggcagcac ggacgacctt
     3121 ggaagttctt ttgacctacc caggtgtcaa ggccttggct gcccaccgtc tgtcccattg
     3181 gatgtggaaa aaaggcttta aattactagc taggatgcac agccaatttt ggcgtttttg
     3241 gactaatatt gaaattcatc caggggcgga tattgcttca ggtgtcttca tcgaccatgg
     3301 agctggtttg gttatcggag aaaccgccat tattgaatca ggtgtcatgc tttaccacgg
     3361 agttacgctt ggtggaacgg gcaaggatac aggcaagcgc cacccgactg tgcgaaaagg
     3421 ggctctggtt tctgcccacg ctcaagtcat tggtcccatt gagattgggg aaaatgccaa
     3481 agttggtgca gctgccgttg tcctagccga tgttccagca gatgtgacag tggtcggtat
     3541 gccagccaaa atcgtccgtg tacacggtca aaaagatgag aaggtcattc atgacatgga
     3601 agatggtcgt gaacactaca cgactaaatt agcagagctg agagaagcta gtcaccgttc
     3661 atcgcacttg taggaaagaa ggttgacgtg ctactagaag aatttgaaaa tacctctgca
     3721 gtcattgaac cgacggatat ggccattcgt ggtggcggag aggtttgtga gaccatgatt
     3781 ttctccttca atggggaaat tgtggaccgt gtccgtcagt tacctgagag cagagaaggt
     3841 ggctatttag aaagcattaa tggtcgtcat ccttggtata ttttggaaag agatggggtg
     3901 cgagttgcgg tcatgttggc agtaatcggt gctcccatgg cagttggtca cttggaggaa
     3961 ctgaaagcct gtggctttga gaattttatc gttcttggct cttgtggggt gctggataag
     4021 tcgcttgttg cggataagat tatccttcct agctctgccc tgcgggacga gggaactagt
     4081 taccactacg ctcctgccag tgatgaaatc agctatgacc ctgctctgct attaaccatg
     4141 gaaaaggcct tggatcaagc gggaatcgag cacgtgcggg ccaagacctg gacgacggac
     4201 gctttctatc gggaaacgtc tgccaaggtc aaacgtcgcc tagccgctgg tgctatggtg
     4261 gtcgatatgg aagcttctgc tatcatggcc tgggcaaact ttcgtcaggc caaggtctat
     4321 caatttttct acacggcaga ctatgtggac taccacaaga atgaatggga tgcccgtcga
     4381 gaggaacgga cggctgacag tatgaccttc tttgaagtgg ccatggcgat tgcaagaaaa
     4441 ttagaaagta catcatgtta aaaaaattag taagacaaaa ctggccttat gtgttgaccg
     4501 ccattgctgg gacaatcatg tttatcctta aattctccca aggaagctgg caggtaggga
     4561 tgatttggtt ggcagcgaca gcttattggc tagtaaaatt gtatcaaaag tatcaagtcc
     4621 taaagaatac tcagaagtaa tggagaaact atgattaaaa tttacgatac catgactcgc
     4681 agtctgcgtg actttgtgcc cttggaagag ggtaaggtca agatgtatgt ctgcggtccg
     4741 acggtttaca actatatcca tatcggtaat gcccgtagtg tcgtggcttt tgataccatc
     4801 cgccgctatt ttgaataccg tggctatgat gtgacctaca tttcaaactt tacggatgtg
     4861 gatgataaga ttatcaaggc tgctaatgaa gctggcatga caacaaagga actttctgat
     4921 aaatttattg cagctttcaa ggaagatgtg gcaaaactgg gtgtcaagcc tgctaccaaa
     4981 aatcctcgtg tcatcgacta catgggtgaa atcatcgatt ttgtgcaggt cttgattgac
     5041 aagggctacg cctatgaagc ttctggagat gtctatttcc gtgtggaaaa ggcggagaat
     5101 tatgctcgtt tggccaacaa aaccctgtct gacttggagg ctggtgcttc tggtcgagtt
     5161 gagggagaag gtcaactgaa agaacatcct ttcgactttg ccctgtggaa aactgccaag
     5221 ccaggtgaag tgtcttggga aagtccctgg ggggcaggtc gtccaggttg gcatatcgag
     5281 tgttcggtta tggcgacaga gattttggga gctaccattg acattcacgg tgggggagcg
     5341 gacttggaat tccctcacca taccaatgaa atcgcccagt ctgagtgcaa aacaggtcaa
     5401 acctttgcca actactggat gcacaatgcc atgctcaata tcaacgatga gaagatgtcc
     5461 aagtccttgg gcaatttcct gacagcccat gacatgttgg aacaaattga cggtcaggtc
     5521 ttgcgcttct tcctatctac ccagcactac cgccgtccac tcaactacac tgagaaggcc
     5581 atttcagatg cggaaatcaa tctaaaatat ctgaaaaata cttatacaca acctgtgcaa
     5641 aactctgtgg ataagtcagc tcttgaatta catctggcgg cctttgaagc ggcaatggat
     5701 gatgatttca atgcggcaaa tgggattacg gctatctttg actttgccaa atggatcaac
     5761 tcaggcaatt acgatgaaac tgtaaaagaa gcctttggca aaatgctaga agtgttcggt
     5821 attgtctttg aagaagaagt attggacagc gaaattgaag ccttgatagc agaacgtcaa
     5881 gcagcgcgtg ccaatcgaga ctttgcgacg gctgaccgta ttcgtgatga attggcagcc
     5941 cagggcatca agctcttgga taccaaggat ggtgtgaggt ggacacgtga ctaaggcagt
     6001 ggatgtcaat ctcatcaacg gaattgccct ggcttttgaa ggggatgctg tctattccat
     6061 gtatgtcaga cggcacttga ttttcaaggg gctgaccaag cctaacaaat tgcatggcga
     6121 ggctaacaaa tacgtctcgg ccaaggctca ggcaggcttg atttctgccc tcttagaagc
     6181 tcagctattg accgaaaaag aggaagagat ctacaagcgg gggcgcaata ccaatagcca
     6241 caccaaggcc aagaatgcgg acgtggtcac ctatcggatg tccacaggct ttgaggcggt
     6301 cttgggatac ctgcacatga cagagcagac ggagcgcctg gatgaactgg taggttggtg
     6361 tattgacaat atagaaagag gataagatga acgagaagga acttgcaggt tggtttccgc
     6421 aggctaagtt atcgcaggaa gcggacgcac agtcgcttgc tttaggcgac ggtcgctatc
     6481 tgaatctggc ggatgcggat ttgacagagc gagaacgcta tctcttgtcc ttgctgtccc
     6541 atcctcaaga gcagaaaccg tcaagccctt ggcagtccta tctacatgga cagagctctc
     6601 tgccccaggc ggttgaagct attcaattta tccatgtcca cctttggtcg cagaaggacg
     6661 cccagttgga tgcatggcag accatgatga ccgacctcct acccagtcaa ttggctcagg
     6721 tacaactgtc gagtcaggat taccttttta tcatcgaaca aagtcgttgg acggactatc
     6781 aaaccattct ggcagacacc ctatcagcct tggagtttga ctttggcttg cggttgacat
     6841 tcctagtcgg tcaggtttgg tctcgtcaac ttgcagatcg ttggtctcaa ctctatcaag
     6901 cagaagcagc cctctttgct cactggcgag cgcactacca tcaatcaacc gtcctatcct
     6961 ttagtcaact ctttctttgg ggacaaggca gagcaggttt ggagtttggt atgataaaag
     7021 ctagtatgct acagttgatt gaccagcaag atatgcagga catcattcta gccctgtgga
     7081 cagaaggagc cgtggtcacc aagaccgctc agatcctcta tatccatcgt aataccctcc
     7141 aataccgatt ggacaagtgg caggatttga ccgggctcca gctcaaagac ctgactgatt
     7201 taagtttttg ttatcaagta ttactagata ggctttttta atcttttgtg caacttgtac
     7261 aaaagattgt gttctttttg ttcaccttga cctagaaaaa taaaacgctt tcatttataa
     7321 taaaactatc aaagaaagtg aggcttgaac atggttcaac ttaatttaaa aaatatttat
     7381 aaaaaatatc caaatagcga gcattattcc gttgaaaact ttaatctaga tattaaagat
     7441 aaagagttta ttgttttcgt gggcccatca ggatgcggta aatcaactac tcttcgtatg
     7501 attgctggat tggaagatat tacagaaggt gaagcttaca tcgacggtgt gttgatgaac
     7561 gatgtagcac caaaagaccg tgacattgcc atggtattcc agaactatgc cctttaccca
     7621 cacatgactg tatttgacaa catggctttt ggtctgaaat tgcgtaaata cagcaaggat
     7681 gaaatcaaaa aacgtgtaga agaagcagca caaattctag gcttgactga attcttgcaa
     7741 cgtaaacctg ctgacctttc tggtggtcaa cgccaacgtg ttgccatggg ccgtgccatt
     7801 gtccgcgatg caaaagtatt cttgatggat gagcctttgt caaacttgga tgccaaactg
     7861 cgtgtatcca tgcgtacaga gattgctaaa atccaccgtc gtattggtgc aacaacgatc
     7921 tacgtaacgc acgaccaaac agaagccatg accttggctg accgtattgt tatcatgtct
     7981 gcgactaaga atgaagcagg tacaggtaca atcggtcgta tcgaacaaat tggtagccca
     8041 gaagagcttt acaataaacc tgtaaacaaa tttgttgcca gcttcatcgg tagccctgca
     8101 atgaacttct ttacagtgac tcttcaagag ggcgtgcttg ttggagatgg tttcaaagta
     8161 gatcttccag aaggtcgtcg caagcatttg gaagaaaaag gctacaacgg caaatcattt
     8221 acattgggta tccgtccgga agacatcaaa gcttcacaat tggagttgga cacttatcca
     8281 tcttctatcg tggaagcaga agttgtcgtt tcagagcttc ttggtgcgga aaccatgctc
     8341 tattctaaag tagggaatac agaatttgta tctcgtgtag atgcgcgtga ttaccacaat
     8401 ccaggagaca aggttcgctt gaccttcaac ctcaacaaag ctcatttctt tgatgatgaa
     8461 acagatcaag ccattgttta atcttttata atttgttcat aaaacaccaa agacccgcta
     8521 ttatgacggg tctttttcat ctttatagac ttcataaaaa tcaaccttga ggcgaataaa
     8581 ggcttccata tcatgcagga gttggaagag ggtggcacgg gtttcaaatt cttcccttgt
     8641 ttgtggcaga ggtcgctcgc ggaaggtggc atggaagagc tcgatgtcta ggagcagttc
     8701 cttggcggag ttttctcgac tcagttgctg ggctgctcgc tcgaagaggc tggacaggat
     8761 gacattttct ctgccttcca gtaaacattt gttgatattt cctgccatgg tccgcaggat
     8821 tttattttgt gcggcccgca tttcaaagta atggacctgg taattggttt gctggaagag
     8881 ttggttgtgg cggtccagat agacgacttt cagagcttgc tccaaggtct gatccaactg
     8941 cgtaatcaat tcagcctcgt ttcgtccgtc tccgttaagt agaaaggctt caaagcgaag
     9001 cagaatctgc ttgagcaggt cttcgacttg gtcgtggtag gcctggattt tcttttcttg
     9061 cgagggcata tagaggttaa acaggagtgc cagtcctgcc ccaatcagaa aaagcgccag
     9121 ctcatttccc aaaaaaatca gcgaaatatc ttgctccagc aagaggtggg taacgaggac
     9181 ggtcgagggg gcgatgccag cctcccagtt gaaacggtag gccaggggga cgtagagggc
     9241 taggtaaatg cccagggtcc agatgccaaa gccaaagagg gcgaaggtca ggacggctat
     9301 ggtcagggcc aggagggtgg aaaagaggcg gttgcgagcc attttgaagc tggacttgcg
     9361 ggtgtcgagg acgctgagaa tggcgatgat gcctgccgct gtcgtataag acaggcccag
     9421 tacggtcgct aggtaaatag ccagaacggt ggcaaatatc agtttgatgg tgcggaggga
     9481 gagggacatg agggctcctt tggttttagt ctatttctat tgtatcataa aataaaaacc
     9541 tcctaaggat aggagggaat tacttatgca aatagagcca gaataccgac tactaggtag
     9601 cagactagtt tgtagctttc gttgatgagg ataaggttga tagggcgttg ctcaaagacg
     9661 tagtttttca atgaagcgag gaccgcaatt acgcccatgc caccagctgc atgaagtggt
     9721 gaaacaccga gggtcttgat gaagaaaata gccacaacag cagtcagtac ttcaatgaca
     9781 agggaaatca ccatttcctt ctgtccattt ttgccagcct gacgatctgc ttctacctta
     9841 gccatgtcaa tgcctgaagc cttgatccaa gcatcgcgga aaatcaaacc ataccacaaa
     9901 ccaccaatag caaaggcaat cagccccgcc acaattccaa aaataagtgt cataaaatct
     9961 cctttttcga ttccgtttgt acggacaaat tgatagaact aaggataaca tagattgtat
    10021 aggcggtcaa gtttttcagc tggaacacag tgagcgccta ttttcagtca acctatgcta
    10081 taataaaacc atggaacata atgatatcgt atatggcgta catgctgtag tggagagttt
    10141 ggaggccaac accggcaaca aactttacat tcaggatgat ttgcgcggaa aaaatgtaga
    10201 aaaaatcaag gcgctggcgg cagagaagaa ggtgtcgatt tcttggacgc ccaagaagac
    10261 cctgtctgac atgacagaag gtgccgttca ccaaggcttt gtcctgcggg tctctgagtt
    10321 cgcctatgcg gacctgtcgg tgattttgga aaaggcagag caggaagaca atcccctcat
    10381 tctcatcttg gacggtctga cagaccccca caatttcggc tcgattttgc ggaccgcaga
    10441 tgccactcag gtagctggca tcatcattcc caagcaccga gcagttggtg tgacgcccgt
    10501 tgtggcaaag acctcaacag gtgcggtggc ccatgtccca attgcccgag tgaccaatct
    10561 tagccaaacc ctcgacaaac tcaaggaggc tggtttctgg gtatttggta ccgatatgaa
    10621 cggaacccca agccacaagt ggaatacggc tggcaagcta gctctcatta tcggcaacga
    10681 aggcaagggc atctccagca atatcaaaaa gcaggtggat gagatgatta ccattcctat
    10741 gaagggccat gtccaatctc tcaatgctag cgtagcagca gctgtactga tgtacgaagt
    10801 atttcggaag aaaatctaaa tgaaagaaaa agttctcatt gtagatggct acaacatgat
    10861 tgccttttgg caggccacca agcaagattt taaaaaggga gacttggatg cggccagaac
    10921 gaccttgctt cgcaccttgt ctcactacgc agcctttgaa cagattgagg taatttgtgt
    10981 ctttgatgcc caccatgtgc caggtcttcg ccagcagtat gacgaattca aggtatcggt
    11041 catttttaca gaggaggaag agacggcaga tagttatatc gagcggctca gcgcccagct
    11101 caatagtgac cgccgcaagc aggtttccgt cgcaaccagc gacctcaacg aacaatgggt
    11161 ggtcttttca caaggggcct tacgggtttc ggcgcgtgaa ttagaggaga gaaccaggat
    11221 tatcaagaag gacttggaca agtttgtgga ccaggtagaa ctctacaccc cacgactcaa
    11281 tccctggtca gatgggaatt ttcaagcctt aaaagaaatg atggaggaaa tgaaagagtg
    11341 acatttacaa tcgtaaccga ttcgacatcg gatttgccaa tcagctgggt ccaagaaaac
    11401 gatgtgaccg tgcttggttt gaccattaac ctagacggcg tgacttatga aacagtcggt
    11461 gaaaaccgct tgacctcagc agatttactg gagaaaatgg cttctggcag ccaacctacg
    11521 accagtcagg tcaatgttgg tcagtttgaa gaagtttttg aagaggcagc caaggaaggt
    11581 catgaggtac tttatctcgc tttctcagca gctctttcag ggacctacca gagtgcagtc
    11641 attgcacgag agatggtagt ggaccaatat ccagatgcag tcatcaccat cgttgataca
    11701 aaggcagcga ccattggaga aggctactta gtcatgaaag ccgttgaggc acgtgctgca
    11761 ggtaagactc tggcagaaac aaaggcaatc gtagaagatt tggtgcctcg cttacggact
    11821 tacctcttgg tcgacgacct taatcatttg gtgcgaggtg gacgcctgtc taaagcggca
    11881 gccctcatcg gtggattggt caatatcaag cctctccttg cactcaatgc ggaagggaag
    11941 ttagaagcca ttgccaaaat ccgtggtcgt aaaaaaggaa ttaaggaaat gctgaatctg
    12001 accttggata acttggatca ttctacggtc atggtggcct atacgggtga tattgaggcg
    12061 gcagagggcg tcaaatctag tttattggaa gacgaccgta tttctgatgt tctattgaca
    12121 gaattaggac cagttattgc cactcataca gggactggag tcttagctat tttatcaatt
    12181 gggacagcaa tgagagccta gattctaagg gatatttctt gtaaagatgt gtctcttttg
    12241 tacgatgacg acttatgtag catagggtaa tcaaaaggag atacgataat gctcattgtc
    12301 aagttgttcc gaccagaagc cttggatggc tgaaactggt gttttacagg tcttctggtc
    12361 tatacggtgg atgaattggt cttggaagaa tttgataaaa cctttcctta taaggagaag
    12421 ctggtcttgc ctggacaact agggcagtag ggaaagtaaa cctgataagg ctttcattct
    12481 cagcagctct gctatctttc aaaaaataaa aaaagtaaag taaaatagct tgacggagac
    12541 tagtagattt gataaaatgg tagacggtat tgtttacccc atttgaaagg ccccggaacc
    12601 ttccaaatac ccgcggaccg gaacatccgc ccagtaaaca aaaacgattc gtataggaga
    12661 aatcatgaac aaaacaacat ttatggctaa accaggccaa gttgaacgta aatggtatgt
    12721 agtagacgct actgatgtac ctcttggacg cctttcagca gtagttgcta gcgttcttcg
    12781 cggtaaaaac aaaccaactt tcacacctca cactgatact ggtgacttcg ttatcgttat
    12841 caacgctgag aaagtgaaat tgactggtaa aaaagcaact gataagattt attacactca
    12901 ctcaaaccac ccaggcggat tgaaatcaat ctcagctggt gaattgcgtt ctaaaaacgc
    12961 tgttcgtttg attgaaaaat cagttaaagg tatgcttcca cacaacacac ttggtcgtgc
    13021 acaaggcatg aaattgaaag tgtttgtggg tggcgagcac actcacgctg cacaacaacc
    13081 agaagtactt gatatttcag gtcttatcta agggaaagga cgtaatctaa atggcacaag
    13141 cacaatacac aggtactggt cgtcgtaaaa acgcggttgc acgcgtacgt ttggtcccag
    13201 gtactggtaa aatcacagta aacaaaaaag atgtagaaga gtacatccca cacgctgacc
    13261 ttcgtttggt tatcaaccaa ccattcgcag taacttcaac tcaaggttca tacgacgttt
    13321 tcgttaacgt gaacggtggt ggttacggtg gtcaatcagg tgcgatccgt cacggtatcg
    13381 cgcgtgcatt gcttcaagta gacccagact tccgcgattc attgaaacgc gcaggccttc
    13441 ttacacgtga cgctcgtatg gttgaacgta agaaaccagg tcttaagaaa gcacgtaaag
    13501 ctagccagtt ctctaaacgt taattatcag cgatatatca acgtttcaaa gcactcgcag
    13561 ggttttccct acgggtgctt ttttctgtgc tttttacaaa agtttgccct aaaatttgcc
    13621 ctaaatttgc actactcatt tttaagatgt tgataggcga catgaccagc agatagaggg
    13681 acaatattag aagtgttgac ataggtctta gttgtgagag tatcactatg agttaatgct
    13741 tctgagatag cttctaagct cattccagct ttcttagcaa gtgttgctcc agtgtgtcgg
    13801 agtttatgag gtgtggcatg tgttagctta ggatgtcgct tcttaatggt cttcatttta
    13861 ttattgagat agtcagcatg caaaggctta ttgatgtttc ctttggtatc aatataagtg
    13921 aagacgaact gttcaggatt cgtaataatt ccaaactttg caagttcctg tttttgttgt
    13981 gatttccaac cttggagaag ttgaagcaag tcagttgaaa tagcaaagat tgttttcttg
    14041 ttccctttgg ttgatttcac atctccatac ctatctagtg cttggagtaa ttgaatttgg
    14101 gatttttcaa aatcaatgtg tttccactgc aaagcataag attcagattt tcggtcgcct
    14161 aaaaagaagg taagataaaa tagaacatag tccttaagtg aaatttttcc gctagccaaa
    14221 tcatcttgga aggctgagaa ccattcctgt aattgctctt gtgttaggta gaggtcttca
    14281 tctcgcctag cttctgccaa ctggattttt ttagttgatt tgatacgacg taaggtttta
    14341 gcgattttat ttgattctat gtactctaat tcttctgccc aatcaaagat ggatatgaca
    14401 tagctacgta aagttttgaa gttcgcatat tcacttgctt ttgctgccat taaatttaga
    14461 ataacctgct tgttctgatt gagaaattga acagtataat tacctaacat aggtaaaata
    14521 tgttttctga aacaagtttt tgtattagct accgttgatt tgctaggtgg tttagttgtt
    14581 gaagtagtct gtcctgcttt gtaggcttcc caccaaatag attcgtagaa atctttgaat
    14641 agaatgtctc ccttacctat ccctgtaaac tcgttgttct caatctgatt gatttttgtt
    14701 aaaaggtcta tctctgcttg ccgtgcctcc tttctggtct tgaagcgctt atcaaaatag
    14761 ttattattga cgataccaag tggcatccgt gtttccgttg gaatatacac ttttaagcga
    14821 tatgttccat tttttgtctt agtgatagtc atgatattct cctttatgtc gaaccagaaa
    14881 atatcatgac cattataaaa caaaaatgtg gatatttata gttttgtgaa agtagccatg
    14941 agtcgatggc ttgtttgctg aaacgaatcg tttttccgat cttaatagat ggaagtcctt
    15001 tgcgaatcca ttcatccaac gtgttatttg aaattccaag gtagtcacat gcttgttttt
    15061 tgtttaagta tgaattgtct atatttcgat gatgaatttg tttttcaatt tctgatgtaa
    15121 ttaaacttga aattaatatc tgaatttgtt tgatttgatc gtctggtaag ataacttgca
    15181 ttttaacctc ttttctcgta aaatttgcaa ttgttttttg acattagtta ttatgccata
    15241 gtgtggacta aaatc
//
LOCUS       DBNFEG010000034        15051 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.034, whole genome shotgun sequence.
ACCESSION   DBNFEG010000034 DBNFEG010000000
VERSION     DBNFEG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 15051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15051
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.034"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            105..554
                     /locus_tag="AC1PNG_000732"
     CDS             105..554
                     /locus_tag="AC1PNG_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIP6701252.1"
                     /translation="MGHTIADFRNLLNQIEQISETIAKEYDVEHLAGPQGWALRFIAE
                     RSEVETFVKDIEAELRISKSVASNLVKRMEKNDFIRVLPSQFDRRYKQLVLTEKGRSK
                     ICHLKSFHEEMHHSLFRGIQKEEFDLIRQVADQLKDNIQHYKEKNHV"
     gene            547..2253
                     /locus_tag="AC1PNG_000733"
     CDS             547..2253
                     /locus_tag="AC1PNG_000733"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6701253.1"
                     /translation="MFKEAILRYKWYALASVLLTSIVVATTLMQPSYLQDVLTAVLAN
                     DKEEIVRVGKFLLLIAGIGLLAGLTNTIMAAKISQGVSADIREKTFRKIQSFSYANVE
                     EFNAGNLVVRMTNDVNQIQNLVMMLFTILMRVPLLFVGAFIMAVRTLPELWWIIVLMV
                     IIIMGIMAVVMGLMGPRFGKFQSLMDRMNSIAKENLRGIRVVKSFVQEKNQYAKFKEV
                     SNELLDLNLFIGYGFSILQPLMMFISYMAIFVSLYLVSGMLDTNIEAVGGFTSFMSYL
                     MQIMFAIIMTSFMGMQASRAAISIKRLSEVLDTEPAMTFKDGAKEDLDGRIVFDNVSF
                     TYPHDTEPTLKNISFEIESGQMVGVVGATGAGKSTLAQLIPRLFDPQEGTVSIGGRDL
                     KDISQETLRDTVSIVLQKAILFSGTIADNLRQGAPGADLERLERAAGIAQAKEFIDRL
                     DDTYESQVEERGNNFSGGQKQRMSIARGVISDPKVLVLDDSTSALDAKSEKLVQEALN
                     HDLKGTTTVIVAQKISSVVKADKILVLDEGRLIGQGTHAELVATNDVYREIYETQKGR
                     EE"
     gene            2254..4038
                     /locus_tag="AC1PNG_000734"
     CDS             2254..4038
                     /locus_tag="AC1PNG_000734"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6701254.1"
                     /translation="MKTLRFFWFYFKRYKLSFAVIFLAIVAATYLQVKTPVFLGNAIA
                     EMGKIGQAYFMANQAGQADFQPDMADFNGVMLNLFFAYAATVVASLIYTLLFTRIVAH
                     STNRMRKGLFGKLERLTVAFFDSHKDGDILSRFTSDLDNIQNAFNQSLTQVVTNIALY
                     VGMVIMMFRQDTRLALVTIASTPVALIALVIIIRLSRKYTDKQQAAVSKLNAYMDEKI
                     SGQKAIIVQGVQEETIDGFLELNEEVRRTTFKGRLFGGILFPFMNGMSLVNTAIVIFA
                     GSSIVLNDSSLETAAALGLVVTFVQYSQQYYQPIMQVAASWAELQLAFTGAHRIQEMF
                     DEPEEVRPQNAPLFIELKEGVEIKDIDFGYLPGQKVLDKVSISAPKGKMVAVVGPTGS
                     GKTTIMNLINRFYDVNGGSVAFDGRDIREYDLDSLRDKVGIVLQESVLFSGTIADNIR
                     FGDESISQEMVETAARATHIHDFIMSLPEGYETFVTDDENIFSTGQKQLISIARTLLT
                     DPQVLILDEATSNVDTVTEAKIQKAMEAIIAGRTSFVIAHRLKTILNADEIIVLKDGK
                     VIEQGNHSQLLKLNGFYAELYHNQFVFE"
     gene            4140..4583
                     /locus_tag="AC1PNG_000735"
     CDS             4140..4583
                     /locus_tag="AC1PNG_000735"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIP6701255.1"
                     /translation="MKIRGFELVSQFTDENLLPKRETAHAAGYDLKAAERISLEPGEI
                     KLVPTGVKAYMQANEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPANEGHIFAQM
                     RNITEETVVVEAGERMVQAVFAPFLLADGDQADGVRTGGFGSTGK"
     gene            4590..5123
                     /locus_tag="AC1PNG_000736"
     CDS             4590..5123
                     /locus_tag="AC1PNG_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIP6701256.1"
                     /translation="MQILFVRHSEPDYSMFDQHDNPRLYAGFGRDLAPLTEKGRTLAQ
                     EIASNPIFSQAQVVVASSVTRALETATYIAHAQQLPLLVEPFFHEWRPDMTGQNASQD
                     EAVLAHRLFLENSGAVPESSPVRYETAAEMRERFLQALEKYKAYDRIVIVCHGMLIRQ
                     FVPKETIAYCEILEYTL"
     gene            5130..6500
                     /gene="radA"
                     /locus_tag="AC1PNG_000737"
     CDS             5130..6500
                     /gene="radA"
                     /locus_tag="AC1PNG_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIP6701257.1"
                     /translation="MTIAKKKTTFVCQSCEYHSPKYLGRCPNCGSWSSFVEEVEVAEV
                     KNERVSLTGEKTRPMKLNEVSSIQVARTKTNMEEFNRVLGGGVVPGSLVLIGGDPGIG
                     KSTLLLQVSTQLSTIGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSIRTE
                     IEKIKPDFLIIDSIQTIMSPDISSVQGSVSQVREVTNELMQIAKTNNIATFIVGHMTK
                     EGTLAGPRTLEHMVDTVLYFEGERQHTFRILRAVKNRFGSTNEIGIFEMQSQGLVEVL
                     NPSEVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRAS
                     LIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVALASSYKDKPTNPQECFI
                     GEIGLTGEIRRVNRIEQRINEAAKLGFTKVYAPKNSLTGIKVPKEITVISVTTIGEVL
                     QKVFNL"
     gene            6621..7115
                     /locus_tag="AC1PNG_000738"
     CDS             6621..7115
                     /locus_tag="AC1PNG_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HIP6701258.1"
                     /translation="MSYFQNFMKANKAYVDLHGDYHLPLRPKTKVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQLGTREIVVLHHTDCGAQTFTNEEF
                     AVQLKRDLGVDVAGQDFLPFTDIEESVREDIALLKQSPLIPDDVEISGAVYDVDTGRM
                     TEVI"
     gene            7323..8081
                     /locus_tag="AC1PNG_000739"
     CDS             7323..8081
                     /locus_tag="AC1PNG_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HIP6701259.1"
                     /translation="MSDNRMKYTIDSNMQFPLVEIALEAGESAYIQQGSMVYHTPSVT
                     LNTKLNARGGSGFGKLMGAIGRSMVSGESMFITQAISNSDDGKIALAPSTPGQVIALE
                     LGAEQYRLNDGAFLALDGSAQYKMERQSVGKAFFGGQGGLFVMTTEGRGTLLANAFGS
                     IKKLTLDGGSITIDNAHVVAWSRDLDYNIHMENGFLQSIGTGEGIVNTFTGYGEIYVQ
                     SLNIETFAQVIGNHIVGAGGDGGGGSTSLLDAIF"
     gene            8259..8705
                     /locus_tag="AC1PNG_000740"
     CDS             8259..8705
                     /locus_tag="AC1PNG_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HIP6701260.1"
                     /translation="MKVRIEFDDSLDHVEVVIRTSQLGPEIEQIQQALQQVSRPSLVF
                     YKGSSEYFLSLGDILFFETDGTKIFAHTGDDAYEVKMKLYELGEHLPIYFCRVSKSTI
                     ANARAVYSLDKSFSGTSRISFYQTHKEVHVSRHYYHLLKEKLREVR"
     gene            8710..9369
                     /locus_tag="AC1PNG_000741"
     CDS             8710..9369
                     /locus_tag="AC1PNG_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF transmembrane domain-containing protein"
                     /protein_id="HIP6701261.1"
                     /translation="MKRLLIGAAVIGLAVLVLLRDSLGLHLAMPFWTTVWFVLTFVTG
                     FHRLVKLDWLASIFCFGLAFIAANSVYEWVEFGTWNMFLSLVLLCLGLKLIFKPRKRR
                     LAHGVVTGVIGDKGRDTAFGSSTRYIHEENFIHDSVDVAFGSSTVYFDQAVILGNKAD
                     FHIDAAFSNITLYLPADWQAVVELDSAMSTTNNYASEPKEKVLLVTGDMAFSTLNIYS
                     M"
     gene            9461..10741
                     /locus_tag="AC1PNG_000742"
     CDS             9461..10741
                     /locus_tag="AC1PNG_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP6701262.1"
                     /translation="MKKILKNATYLWVLTADMLSNFGDVVYYLALMNYVLLVPNSRLA
                     LAIVTFSEIFPSFMGLFTGYLADKTVNKISTIKLTLLFRVLLYLILGFCMGFEPALWI
                     VVVAATFNVFADFAGFYENGLYTPLGLRVAPKEEREQYSAFRQTVTSLLNIAFQALSA
                     LLVGLLSYQNLAFLNAGTFLAALLIMQLLTPVFRKLLTEQPLKIAEQSSQTEQKTKRP
                     GILASFKQAIVELRKIPEFRLVLITTPLINACGAILYPILVLLISEDPGLVFLNVETT
                     LALTILIFFIGHILGSTLVFTLFKNTSMVTLEVAATFSLLGVFVGMLLHQLPIIFFFL
                     TTMGISSGASGPKFNAKFVNSMPEEQLATIGGAVSTYFMLGQAFTRLLVSGLVLILTV
                     NQISGLFLAATGLLVLYILYWLARNQKTPQNQSV"
     gene            10813..12267
                     /gene="gltX"
                     /locus_tag="AC1PNG_000743"
     CDS             10813..12267
                     /gene="gltX"
                     /locus_tag="AC1PNG_000743"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIP6701263.1"
                     /translation="MTKPIRVRYAPSPTGLLHIGNARTALFNYLFARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGIDWDESPETHEQYRQSERLDIYQKYVDQLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEYLGMSEDEKAAYIAEREAAGIVPTVRLAV
                     NETGIYKWNDMVKGEIEFEGGNIGGDWVIQKRDGYPTYNFAVVVDDYLMKISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRRKGYM
                     PEAVFNFIGLLGWNPGGEEEIFSREQFIQLFDENRLSKSPAAFDQKKMDWMSNEYIKN
                     ADLETIFNLAKPFLEEAGRLTDKAEKLVELYKPQLSSADEIVGLTDLFFSDFPELTAE
                     EKEVMAGETVPTVLNALKEKLEAMTDEDFQPDNIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPNTIYLLGREKSIQHIDNMLKSL"
     gene            complement(12322..12822)
                     /locus_tag="AC1PNG_000744"
     CDS             complement(12322..12822)
                     /locus_tag="AC1PNG_000744"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIP6701264.1"
                     /translation="MEIWNTYTADGHLTDHTLTRGESIPDGLYHLVVECIIRHHDGST
                     LFMKRDSTKPSYPDYYEATAGGSALFGEIAEQAILREVREETGIELTADQLRHHTHFV
                     AHDDQCIFHCYWAETDWDKSTIQLQSNETSNYIWVPQENLKNFLETELVIPRQKDYVE
                     RLFLKS"
     gene            12943..13818
                     /locus_tag="AC1PNG_000745"
     CDS             12943..13818
                     /locus_tag="AC1PNG_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIP6701265.1"
                     /translation="MQTIIPHLWYDTEAKEAVAFYVELFGGKIDWTYTITDTPSGDSD
                     LIQFQLGDMTLAAISAGPYFKLNESMSLMVNVASKDEVTRLYQVLSEGGRVLMPLGEC
                     PFSPYYVWLEDRFGLSWQLSYAPDLDKPYQFDICLLFSQEQVGLAQPMLDYYKDKLHQ
                     ARLGRLSYYGEGEASVAAAKLNYAELFIGDQKIIAMDHGYGGVASFNEAFSFMVYVDS
                     QEEAESWYEKVSAVPEAEICGWAKDQFGISWQIVPRILMEAYDTANPEKVKAVNAAVM
                     TMKRLDIAAIQALLN"
     gene            13924..14442
                     /locus_tag="AC1PNG_000746"
     CDS             13924..14442
                     /locus_tag="AC1PNG_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HIP6701266.1"
                     /translation="MKATERRKKILSLLRESPYPLAANYLAKEFGVSRQVIVGDVALL
                     RAEGEAVLATPRGYLLQSSLPSTEGAYIRKIACQHGPEEVEKELTILLEGGAQILDVE
                     IEHPIYGLLSGKLNIASLEDRDHFLDALGQYQGVLLSNLTEGVHTHTVSFPSVQAYNE
                     LSAALKHAGILL"
     gene            14522..>15051
                     /locus_tag="AC1PNG_000747"
     CDS             14522..>15051
                     /locus_tag="AC1PNG_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701267.1"
                     /translation="MSQLTLSSKNSVKQLSISAILTAFAILIPLMMPIKIIIGPASYT
                     LASHIPLFIAMFISPATAIFVALGSSLGFFLAGFPIVIVFRALTHLFFLTLGAVLVKR
                     FPTLMDSKRFLLLGIGLNLLHGLGEYIVVMVLTSGQQTSATYWITMLGLVGVGSAIHG
                     LLDFSLAYYFWKILKER"
BASE COUNT         3876 a         3180 c         3708 g         4287 t
ORIGIN      
        1 ataacaaaat attttcagaa aagagcaaac ggaataattg acaatagttc ctaagagaac
       61 tataatagtt ctcatggtaa ttattttcgt agaaaggagc tattatggga catactattg
      121 cagattttcg gaatctatta aatcagattg aacaaatcag tgaaaccatt gcaaaggaat
      181 atgatgtgga gcacctagct ggaccacagg gatgggctct gcgtttcata gcagaacggt
      241 ctgaagttga aacctttgtc aaagacattg aagcggagtt gaggatttcc aaatccgttg
      301 ccagcaatct agttaagcga atggagaaaa atgactttat tcgggtttta ccttctcagt
      361 ttgacagacg ctataagcag ttggtcttga cagagaaagg tcggagcaag atttgtcacc
      421 taaaatcctt ccatgaggag atgcaccatt ctctctttcg aggtattcaa aaggaagaat
      481 ttgatttgat cagacaggta gccgatcaat taaaagataa tattcaacac tataaggaga
      541 agaatcatgt ttaaagaagc cattttacgt tacaaatggt atgccttggc atcggtcttg
      601 ttgacgtcta ttgtcgtagc aacgaccttg atgcaaccta gttatttaca ggatgtattg
      661 actgctgtct tggccaatga taaggaagaa attgttcgtg tcggtaagtt cctcttgctg
      721 attgcgggaa tcggtctctt ggctggtctg accaatacaa tcatggcagc taagatttct
      781 cagggagtgt ctgcggatat tcgtgagaag acctttcgga aaattcagag cttttcttat
      841 gccaatgtag aggaatttaa cgcagggaat ttggtggttc gtatgaccaa cgacgtcaac
      901 caaattcaga accttgtcat gatgctcttt acgatcttga tgcgggtacc tctgctcttt
      961 gttggtgcct ttatcatggc ggtgcgcact ttgccagaac tttggtggat tatcgtgctc
     1021 atggtcatca tcatcatggg aatcatggca gtcgtgatgg gcttgatggg accgcgcttt
     1081 ggtaaattcc agtccctgat ggacaggatg aacagcattg caaaggaaaa cctccgtggg
     1141 attcgtgtgg ttaagtcctt tgtacaggag aagaatcaat acgctaaatt taaagaagta
     1201 tccaatgagc ttttggatct taacctcttt attggttatg gtttttctat cttgcaacct
     1261 ttgatgatgt ttatctccta catggcgatt tttgtgtcgc tttatctcgt ttcagggatg
     1321 ttagacacta atatcgaagc agttgggggc tttacttcct ttatgagtta cctcatgcaa
     1381 atcatgtttg ccattatcat gactagcttt atggggatgc aggcttcacg tgctgctatt
     1441 tccattaaac gtttgagtga ggttttagat acagagccag cgatgacctt taaagatggt
     1501 gcaaaagagg acttggatgg tcggattgtc tttgacaatg ttagcttcac ctatccgcat
     1561 gataccgagc cgaccttgaa aaatatctcc tttgagattg agtctggtca gatggtcggt
     1621 gtggtcggtg caacgggtgc gggtaaatca acactagctc agctcattcc gcgtttgttt
     1681 gatccacagg aagggactgt gtcaattggc ggtcgtgatt tgaaagacat cagtcaggaa
     1741 accttgagag atacagtgtc tatcgttttg caaaaagcta ttctcttctc agggaccatt
     1801 gcagacaacc tgcgtcaagg tgcacctggt gcagatttgg aacgtttgga gcgggccgca
     1861 ggtattgccc aagccaaaga atttatcgac cgcttggatg atacctatga gagccaagta
     1921 gaagaacgtg gaaataactt ctcaggtggt cagaaacagc ggatgtccat tgcgcgtggg
     1981 gtgattagtg atcctaaagt cttggtgcta gatgattcga catcagcctt ggatgccaag
     2041 tctgagaagc tggtccaaga ggccctcaac catgacttga aggggacaac gacggtcatc
     2101 gttgctcaga agatttcttc tgttgtcaaa gctgataaga ttttggttct tgacgaaggc
     2161 cgtctgatcg gtcaaggtac ccacgcggag ttggtagcga cgaacgatgt ttaccgtgaa
     2221 atctacgaaa cacagaaagg gagggaggaa tagatgaaga cattacgttt tttctggttt
     2281 tattttaaac gctataaact gtcctttgct gtgatttttc tagctattgt ggcagcgacc
     2341 tacttgcagg tcaagacacc tgttttcctt ggaaatgcca ttgcggagat ggggaaaatt
     2401 gggcaggctt actttatggc caatcaagct ggtcaggctg actttcagcc agatatggct
     2461 gattttaacg gggttatgct caatcttttc tttgcctatg cggcgacggt tgtggcttcc
     2521 ttgatttaca ccctcctctt cacgcgtatc gtggctcatt cgaccaaccg tatgcgtaag
     2581 ggcttgtttg gcaagctgga acgcttgaca gttgccttct ttgatagcca caaggacggg
     2641 gatattcttt ctcgctttac cagtgatttg gacaatatcc aaaacgcttt taaccagtcc
     2701 ttgacccaag tggtgaccaa catcgctctt tatgttggta tggtcatcat gatgttccgt
     2761 caggatactc gcttggcctt ggtgaccatt gcttctacgc cagttgcttt gattgcccta
     2821 gtgattatca ttcgcctgtc acggaaatat actgataagc aacaggctgc ggtatctaag
     2881 cttaatgcat acatggatga gaagatttcc ggacaaaaag cgattattgt acaaggtgtg
     2941 caggaagaga caattgatgg tttcttggaa ctcaatgaag aagttcgtcg tacgaccttc
     3001 aagggacgct tgtttggtgg gattctcttc ccatttatga atgggatgag tttggtcaat
     3061 actgccattg ttatctttgc aggttccagc attgttctca acgatagttc gctagaaaca
     3121 gctgccgcac ttggtctggt ggtgactttt gttcaatatt cgcaacagta ttaccagcca
     3181 atcatgcagg ttgctgccag ctgggcagaa ttgcagctag ctttcacagg agctcatcgt
     3241 attcaggaaa tgtttgatga gcctgaggaa gttcgccctc aaaacgctcc gctatttatc
     3301 gaattaaaag aaggtgttga aatcaaggac attgactttg gctacctgcc gggtcagaag
     3361 gtcttggaca aggtgtcaat ctctgctcct aagggcaaaa tggtggcagt cgttggtccg
     3421 acgggatctg gtaagactac cattatgaac ttgattaacc gcttctacga tgtcaatggt
     3481 ggtagtgtgg cttttgacgg tcgtgatatt cgggaatatg acttggacag cttgcgggat
     3541 aaggtcggca ttgtcttgca ggagtcggtt cttttctcag gtactattgc ggacaatatt
     3601 cgctttggtg atgagagcat ttcgcaggaa atggttgaaa ccgcagctcg tgccacccat
     3661 atccacgact ttatcatgag cttgccagag ggttatgaaa cctttgtgac tgatgatgag
     3721 aatatcttct cgacaggtca gaaacagctg atttccattg cccgtacgct cttgacagat
     3781 ccacaagtct tgattttgga cgaagcaacc tcaaacgttg atacggtgac ggaagccaaa
     3841 attcaaaagg ctatggaggc tattatcgct ggacggacca gcttcgtcat tgcccaccgc
     3901 ctcaaaacca tcctcaatgc ggacgaaatc atcgtcctca aagacggaaa agtgatcgag
     3961 caaggcaacc acagccaact cctcaagctc aacggcttct atgccgaact ctaccacaac
     4021 cagtttgtgt ttgagtaaag ttgaaaaccc cagccactag gttggagttt tgcttttagc
     4081 ctacgaataa attataatag agagcgtatt atgcaggaag aaattgaagg gaaactacta
     4141 tgaaaatccg tggttttgaa ctggtcagcc agtttactga tgaaaattta ttgccaaaac
     4201 gtgagaccgc tcatgcagca ggttatgacc tgaaagctgc ggagaggatc agcttagagc
     4261 ctggtgagat taagctggtt ccaacgggtg tcaaggccta tatgcaggca aacgaggtcc
     4321 tctatctcta cgaccgttcg tccaaccctc gcaagaaggg gctggttttg attaactcag
     4381 ttggagttat cgacggcgac tactatggca atccagccaa tgaaggtcat atctttgccc
     4441 agatgcgaaa catcacagag gaaactgtcg tggtggaagc tggcgagcga atggtgcagg
     4501 ctgtttttgc acccttcctc ttggctgacg gcgaccaggc cgacggcgtt cggacaggtg
     4561 gatttggctc gacggggaaa tagactacta tgcagattct ctttgtgcgg cattccgaac
     4621 ccgactacag tatgtttgac cagcatgata atcctcggct gtatgcaggt tttggacggg
     4681 atttggctcc tttaactgaa aaggggcgga ctttagctca ggaaattgct agtaatccta
     4741 tcttttccca agctcaagta gtcgtcgctt caagcgtcac acgggccttg gaaactgcga
     4801 cctacattgc tcatgcccag caactacctc ttctggtcga gccctttttt catgaatggc
     4861 gaccagatat gacggggcaa aatgccagtc aagacgaggc tgtcttagct catcgattat
     4921 ttttggaaaa tagcggagcg gtgcctgagt cttcacctgt tcgctatgag actgctgcgg
     4981 agatgagaga gcgattttta caggcccttg aaaaatacaa agcctatgac cgaatcgtta
     5041 tcgtctgtca cggtatgctg atacgccagt ttgttcccaa agaaactata gcctactgcg
     5101 aaatcctaga atacactcta taggaggaaa tgaccatcgc taagaaaaaa acaacctttg
     5161 tctgtcaatc ctgcgagtac cactcgccca agtatctggg ccgttgcccc aactgtggct
     5221 cctggtctag ctttgtcgag gaagtggaag tcgctgaagt caagaacgag cgggtcagtc
     5281 tgacaggtga gaagacccgt cctatgaagc tcaatgaagt gtcgtccatt caagtggccc
     5341 gcaccaagac caatatggaa gagtttaacc gtgtccttgg tggcggcgtg gtgccgggca
     5401 gtctggtcct gattggaggc gatccaggga ttggcaaatc gaccttgctc ctgcaagtat
     5461 cgacccaact ttcgactatt ggcaccgtcc tctatgtgtc gggggaagag tctgcccagc
     5521 agattaagtt gcgagcggag cgtttgggcg acattgacag cgagttctat ctctatgcgg
     5581 agaccaatat gcagagcatt cggactgaga ttgagaaaat caagccagat ttcctgatta
     5641 tcgactctat ccagaccatt atgagccctg acatttctag cgtgcaaggt tctgtcagtc
     5701 aggtccgtga agtgaccaat gagcttatgc agattgccaa gaccaacaat atcgcaacct
     5761 ttatcgtcgg ccacatgacc aaggaaggca ccctggctgg accgcggacc ttggagcaca
     5821 tggtagacac cgttctctat tttgagggcg agcgtcaaca caccttccgt atcttgaggg
     5881 cggtcaaaaa tcgctttggc tccaccaacg aaatcggtat ttttgaaatg cagtcgcagg
     5941 gcttggtcga agttctcaat ccaagtgagg tctttctgga agagcgtctg gacggagcga
     6001 ctggctcggc tattgtcgtg accatggagg gcacccgccc tatccttgcc gaagtgcagg
     6061 ctctggtgac cccgaccatg tttggcaatg ccaagcggac cacgacaggc ttggatttca
     6121 accgtgccag cttgattatg gcggttttgg aaaaacgggc cggtctgctc ctccaaaacc
     6181 aagatgccta ccttaagtca gcaggcggtg tcaaactgga tgagccagct attgacctag
     6241 cggtcgcagt tgcccttgcc tccagttaca aggacaagcc aaccaaccca caagagtgct
     6301 ttataggcga gattggcctg acaggggaaa tccgccgcgt caatcggatt gaacaacgga
     6361 ttaacgaagc cgccaaattg ggctttacca aggtctatgc cccaaaaaat tccctgacag
     6421 gtatcaaggt gcccaaggaa atcaccgtta tcagcgtgac caccattggc gaagtcttgc
     6481 agaaagtatt caacttatga aatcattcgg gagtgacagc tagtcgctcc tctttttgcg
     6541 acaaatagtc gggaatgtga taagctaggt ctaacctatt tctgaaaatt tcagaaattc
     6601 tcatcagaca aggaggtcct atgtcttatt ttcaaaactt tatgaaggct aacaaggcct
     6661 atgttgacct gcatggtgat taccatttgc ctcttcgtcc aaagaccaag gtggctatcg
     6721 tgacctgtat ggactcacgg cttcacgtgg ctcaggcctt gggcttggct ttgggggatg
     6781 ctcacattct gcggaatgca ggtgggcgcg tgacggagga catgattcgc tcgctggtta
     6841 tttcccagca gcagctcggc acacgggaaa tcgtggtgct ccaccataca gactgcggtg
     6901 cccagacctt taccaatgaa gaatttgcgg tccaactcaa gcgagatttg ggagtggatg
     6961 tggctggtca ggattttctt ccttttacag acattgaaga aagcgtgcgg gaggatattg
     7021 ctctcttgaa acaatctccg ctcatacctg acgacgttga aatttctggt gcggtttatg
     7081 atgtggatac aggtcgtatg acagaagtta tataggaatg ctcaggaagt tcggtttttc
     7141 ctgtaaattt tatctgaatg accgagaaat ccttgacaag tttttttgaa tgagataaaa
     7201 ttgtattatc acaacagaac aagcggagag ataaatgatg cttggtttat tctagcttgt
     7261 tcttggttta ttatggctat cctgtggtta acaggacctt tcgtaaaaaa taggagtcaa
     7321 ctatgtcaga caatcggatg aaatatacta tcgatagtaa tatgcagttt cctttggtgg
     7381 aaatcgcctt ggaagctggt gaatcagctt atattcaaca ggggagcatg gtctaccata
     7441 cgccgagtgt gaccttgaat accaagctca atgcgcgtgg tggttcaggt tttggtaaat
     7501 tgatgggggc catcggtcgg tcaatggtat caggcgaatc tatgtttatc acgcaagcca
     7561 tttccaatag tgacgatgga aaaattgccc ttgcgccttc aacaccaggt caggttattg
     7621 ccttggaact tggggcagag caatatcgac taaatgatgg tgccttcctg gcccttgatg
     7681 gttctgctca atataaaatg gaacgccaga gtgttgggaa agctttcttt ggtggtcagg
     7741 gtggactctt tgtgatgacg acagagggac gcggaacact tttggcgaat gcgtttggtt
     7801 ctattaagaa gctaactttg gatggtggaa gtatcaccat tgacaatgct catgtggttg
     7861 cttggagtcg tgatttggac tacaatatcc atatggaaaa tggtttcctc caatctatcg
     7921 gtacaggtga aggaatcgtt aataccttta ctggttatgg agaaatctat gtacagagtt
     7981 tgaacattga gacctttgct caggtcattg gcaatcatat tgtcggtgca ggtggtgatg
     8041 gtggtggtgg aagcacatcg ttgctggatg cgatttttta agaagaaaaa ctctgcaatt
     8101 ttcaaaatca tataataaaa ataaagtccc tcgcgtaatg caagggactt ttcttagttt
     8161 atttctacca cttacctgtc taattttgcc acttactgaa atgcccttgt aattggatgt
     8221 cgaactggta taatagagct atcaaaaagg agggagctat gaaagttagg attgaatttg
     8281 atgacagctt agatcatgtt gaagttgtta tccgaaccag ccagctggga ccagagattg
     8341 aacagattca gcaagctttg caacaagtta gtcgtccctc gcttgttttt tacaagggga
     8401 gcagcgagta ttttctttca ttgggtgaca tacttttctt tgaaacggac ggaaccaaga
     8461 tttttgctca tacaggcgat gatgcctacg aggttaaaat gaaactctat gaacttgggg
     8521 agcatttgcc catctacttt tgtcgggttt ctaaatcaac gattgccaat gctagagctg
     8581 tgtattcatt ggataagtct ttttcaggaa ctagtcggat ttccttctat cagacccaca
     8641 aggaagtcca cgtgtcgcgg cattattatc atttattaaa agaaaagtta cgagaagtga
     8701 ggtagggaga tgaaacgtct attaattgga gctgcagtta taggactggc ggttttggtc
     8761 ttgttgaggg attcattggg tttgcatttg gcaatgccct tttggaccac tgtctggttt
     8821 gtattaacct ttgtgacagg ttttcaccgc ttggtaaagt tggattggct tgccagtatc
     8881 ttttgtttcg gcctagcttt tatagctgcc aactctgtct atgaatgggt ggagtttggg
     8941 acttggaaca tgtttctgtc gcttgtctta ctctgtcttg gcttgaaact aatttttaaa
     9001 ccaaggaagc gcagactggc tcatggagtt gtcacagggg tgattggcga caagggacgc
     9061 gatacagctt ttggttcctc gactcgttat atccatgagg agaattttat tcatgatagt
     9121 gtggatgtag cctttggatc tagtacagtc tattttgatc aggcggtgat tctgggtaat
     9181 aaggcagatt ttcatatcga tgcggccttc tcaaatatca cactctatct gcctgctgac
     9241 tggcaagcgg ttgtagagtt ggatagcgcc atgtcaacaa ctaacaacta cgcatctgag
     9301 ccaaaagaaa aggttctctt ggtgacgggt gatatggcat tttctacatt aaatatttac
     9361 tctatgtaaa agaataatcg cagaggaaag tctatccagg ttcctctgta aaataaaaat
     9421 tcggaatatt ctggaaatta ttcaaaagaa agggattatc atgaaaaaga ttttgaaaaa
     9481 tgctacctac ctatgggtac tgacggcgga tatgctgtcc aattttggcg atgtggtcta
     9541 ttatctggcc ttgatgaact atgttttgct ggtgcccaac agtcgtctgg ctctggcgat
     9601 tgtgaccttc tcggaaatct tccctagctt tatggggctc tttacaggct atctggcaga
     9661 caagacggtc aacaaaatca gcactatcaa gctgacccta ctctttcgtg ttcttctata
     9721 cctcatcctc ggattctgca tgggctttga gccagccctc tggattgtcg tggtggctgc
     9781 aactttcaat gtctttgcag acttcgctgg cttctatgaa aacggcctct atacacctct
     9841 gggattacgc gtagcgccca aagaagagcg ggagcagtac tcggcctttc gccagaccgt
     9901 gaccagcctc ctcaatatcg cctttcaggc cctctctgcc ctcttggtcg gcctcctctc
     9961 ctaccaaaac ctggcctttc tcaatgccgg caccttttta gccgccctcc tcatcatgca
    10021 gctgctgacg ccagtctttc gcaaactcct gacggagcag cctttgaaaa tagcagagca
    10081 gtcgagccag actgagcaaa aaaccaagag accaggcatt ctagcttcct tcaagcaagc
    10141 catcgtggag ctgcgcaaga tacccgagtt ccgcctggtc ctgattacca cccctcttat
    10201 caacgcctgt ggagcgattc tctatcctat tctggtcctg ctgattagcg aagacccagg
    10261 ccttgtcttc ctcaatgttg aaacaaccct tgccctgacc attctgatct tctttatcgg
    10321 acatattctg ggctcgacct tggtctttac ccttttcaaa aataccagca tggtgacgct
    10381 ggaagtggca gcaaccttct ctctacttgg cgtttttgtc ggaatgttgc tacatcaact
    10441 gccaattatt ttcttcttcc ttactacaat ggggattagt tcaggagcta gtggacctaa
    10501 gtttaacgct aagtttgtca attctatgcc agaggagcag ttggcgacca ttggcggagc
    10561 ggtatcaacc tattttatgc taggtcaagc ctttactcga ctgttggttt cgggtttggt
    10621 cttaatcttg actgtcaatc agattagcgg acttttccta gctgcaactg gtttgttagt
    10681 cctctatatc ctctactggc tggcacgtaa ccaaaaaaca cctcaaaatc agtctgttta
    10741 gactgatttt tctgttatct gggggtgttc tatgctataa tgttcttgat tgaaatttta
    10801 caggagagaa acatgactaa accaattcgt gtgcgttacg caccaagtcc aacgggactt
    10861 ttgcatatcg gaaatgctcg tacagcattg tttaactatc tttttgcccg tcatcacggc
    10921 ggtactttcc ttattcgtat cgaggacaca gaccgcaagc gccatgtgga agacggcgag
    10981 cgttctcagc ttgaaaacct gcgttggttg gggattgact gggatgagag tccagaaact
    11041 catgagcaat accgtcagtc tgagcgtttg gatatttacc aaaagtatgt tgatcagctc
    11101 ttggcagaag gcaaggccta caagtcttat gtgaccgaag aagagttggc ggcggagcgt
    11161 gagcgtcagg aagctgcggg tgaaactcct cgctacatca acgaatacct tggtatgtct
    11221 gaagatgaaa aagcggccta catcgcagag cgtgaggcgg ctggcattgt cccaacggtt
    11281 cgcttggctg tcaacgaaac aggtatctac aagtggaatg acatggttaa gggcgaaatc
    11341 gagttcgaag gtggcaacat cggtggtgac tgggttatcc agaaacgtga cggctatcca
    11401 acttacaact ttgccgtggt ggtggacgat tatctcatga aaatctcgca cgttatccgt
    11461 ggggacgacc acattgccaa tacacccaag cagctcatgg tctacgaagc acttggttgg
    11521 gaagcaccag aatttggtca catgacgctt attatcaatt ctgaaactgg taaaaaactg
    11581 tctaaacgtg atactaacac ccttcagttc atagaagatt accgtcgtaa aggttacatg
    11641 cctgaagctg ttttcaactt tatcggtctt ttgggttgga acccgggtgg cgaggaagaa
    11701 atcttctccc gtgagcaatt tatccaactc tttgacgaga accgcctcag caaatcgcca
    11761 gctgcctttg accagaagaa aatggactgg atgagcaatg agtacatcaa aaatgctgat
    11821 ttagaaacta ttttcaacct tgctaagcca ttcttggaag aagcggggcg tttgacagac
    11881 aaagcagaaa aattggtgga actttacaag ccacaactgt catctgcgga tgaaatcgtt
    11941 ggtttgacag acctcttctt ctcagatttc ccagaattga ctgcggaaga aaaagaagtg
    12001 atggcaggcg aaacagtacc gactgttctc aatgccctca aggaaaaatt ggaagccatg
    12061 acggacgaag atttccaacc agataacatc ttcccgcaaa tcaaggctgt ccaaaaagaa
    12121 acaggcatca agggcaaaaa cctcttcatg ccaatccgta tcgccgtttc aggcgaaatg
    12181 cacggaccag agttgccaaa caccatttac ctgctcggcc gtgagaaatc catccagcat
    12241 attgacaata tgttgaagag tttgtaatga aataatctcc gctgtcctgt gatagtgggg
    12301 atttgtctgt taaggggggg gttagctttt taaaaacaac ctctccacat agtctttctg
    12361 ccttggaatg actagctctg tttccaggaa atttttcaaa ttttcttgtg ggacccagat
    12421 ataattgctg gtttcgttgg attggagttg gattgtggat ttgtcccagt cggtttcagc
    12481 ccagtagcag tggaaaatgc actggtcgtc atgggcgaca aagtgtgtat ggtggcggag
    12541 ttggtcagct gtcagctcaa tgcctgtctc ttcgcggact tcgcgcaaaa tggcctgctc
    12601 tgctatttcc ccaaacaaag ctgaacctcc tgctgttgct tcataatagt cagggtagga
    12661 gggcttggtg ctgtcgcgtt tcatgaagag ggtactgccg tcgtggtggc ggatgatgca
    12721 ctcgacgacc aggtggtaga ggccgtcggg aatggattct ccgcgtgtga gggtgtggtc
    12781 ggtcaagtga ccgtcggctg tgtaggtgtt ccagatttcc atttcattct cctgtcaatt
    12841 ttgtagttat tcagtatagc acatctgtaa gtgcaaagca acatgttttg tttttgtacg
    12901 gacaagtgtt atactggggc tactaactca aaggagaaat caatgcaaac tataatccca
    12961 catctctggt atgatacaga ggcaaaagaa gcggtggctt tctatgtaga actcttcggt
    13021 ggcaagattg actggaccta caccattacg gatacgcctt cgggtgattc ggatttgatt
    13081 cagtttcaac tgggcgatat gaccttggcg gctattagtg cgggtccata ctttaagctc
    13141 aatgagtcca tgtccctcat ggtcaatgta gctagcaagg atgaagtgac acgtttgtac
    13201 caagtcttgt cagaaggcgg tcgtgtgctc atgcctttgg gagaatgtcc tttcagccct
    13261 tactatgtct ggttggaaga ccgctttggt ctgtcttggc aactgtctta tgcaccggac
    13321 ttggacaagc cttatcagtt tgacatctgc ctgctcttct cgcaggaaca ggttggtttg
    13381 gcccagccta tgttggatta ctacaaggat aagctgcatc aagctcgtct tggtcgcttg
    13441 tcttactatg gcgaaggtga ggcttcggtg gcggctgcca agttgaatta tgcggagctt
    13501 tttatcggag accaaaaaat tatcgctatg gaccacggct acggtggtgt ggcttctttc
    13561 aacgaagcct tctcattcat ggtctatgta gacagtcagg aagaagcgga gagctggtat
    13621 gaaaaagtgt ctgctgtgcc agaagcggag atttgcggtt gggccaagga ccagtttggg
    13681 atttcttggc agatcgtgcc gcgtattttg atggaggcat acgatacggc taatccagaa
    13741 aaggtcaagg cagtcaatgc agcggtgatg accatgaagc gtttggacat tgcggctatt
    13801 caagcattgc taaactagca gatgataacg gagactgttc tgcagtttcc gttttttggt
    13861 tgaggaaagg ttgatttata ggaattttga tataatggaa aaaagtgtac gaaaggaatg
    13921 tgtatgaaag caacagaacg acgtaagaaa atcctatccc ttttacgaga aagcccctat
    13981 ccccttgcag ctaattatct ggcgaaagaa tttggagtga gtcggcaagt aattgttgga
    14041 gatgtagcct tgcttcgtgc ggagggagaa gctgtcttgg caactccaag agggtactta
    14101 ctccaatctt ctttgccatc tactgaagga gcttacattc gaaagatagc ttgtcaacat
    14161 ggtccagaag aggtagaaaa agaattgaca atcttgctag agggtggagc tcaaatacta
    14221 gatgttgaaa ttgagcatcc catttacggc ttattatcag gcaagctgaa tattgcaagt
    14281 ttagaagata gggatcattt tttggatgcc ttgggtcaat accaaggtgt gttactgagt
    14341 aatttaacag aaggagttca tacacataca gtctctttcc catctgtgca agcctataat
    14401 gaactttcag cagctttaaa acatgcaggt attttactat aaaatcaata aacattacag
    14461 gtgtcttgac acatgtaatg tttttgtttt ataatcaaac caatagaaag agaggtgcct
    14521 aatgtctcaa ttaacactat catctaaaaa ttcagttaag cagctatcaa ttagtgcaat
    14581 tttaaccgca tttgccattt taataccttt aatgatgccg attaaaatca ttattggccc
    14641 agcatcctat acacttgcta gtcacattcc tttgtttata gccatgttta tttcaccagc
    14701 tactgctatt tttgttgctt taggttctag tttaggcttt tttctcgccg gttttccaat
    14761 cgttattgta tttcgggcgc ttacgcatct gttcttcctt accttaggtg cggtcttggt
    14821 caagcgtttt ccgactctca tggattctaa gcgcttttta ctattaggaa ttgggcttaa
    14881 tctcctgcat ggtctaggag aatatattgt ggtgatggtg cttacttcgg ggcaacaaac
    14941 ttctgctacc tactggataa cgatgcttgg cttggtgggt gttggctcgg ctattcatgg
    15001 attgcttgat tttagtttag cttattattt ctggaaaatt ttaaaagaac g
//
LOCUS       DBNFEG010000035        14293 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.035, whole genome shotgun sequence.
ACCESSION   DBNFEG010000035 DBNFEG010000000
VERSION     DBNFEG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14293
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.035"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            140..2086
                     /locus_tag="AC1PNG_000748"
     CDS             140..2086
                     /locus_tag="AC1PNG_000748"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HIP6701268.1"
                     /translation="MDYKKVSAEVIEAVGKDNLVAAAHCATRLRLVLKDESKVNQKAL
                     DDNADVKGTFSTNGQYQVIIGPGDVNFVYAELIKATGLKEVSTDDLKQIAAQDKKFNP
                     LMDLIKLLSDIFVPIIPALVAGGLLMALRNFLTSPDLFGPKSIEEMYPAIQGISAMIQ
                     LMSAAPFMFLPVLVGISAAKRFGANQFLGAAIGMIMTTPDLGGKEAFWDIFGFQVAQT
                     NYAYQVIPVLAAVWLLATLEKFFHKKLPSAVDFTFTPLLSVMITGFLTFTVIGPVMLT
                     VSDAITNAIVWLYDTTGALGMSIFGGTYSLIVMTGLHQSFPAIETQLLSAYNNNGTGY
                     GDYIFVVASMANVAQGAATLAIYFLTKNAKTKGLSGSAAVSAFLGITEPALFGVNLKY
                     RFPFFCALAGSAIGALFAGLTHVIAVSLGAAGFIGFLSIKASSIPMYVVAEVIAFAAA
                     FAFTYVYGKTKASAVFADEAATAAAAAVTASVVETSEEVEETAEIFTAQETLVSPLSG
                     DVVALENVNDPVFSSGAMGKGLAVKPSEGVVYAPADAEVTIAFETGHAYGLKTASGAE
                     LLIHIGIDTVSMNGNGFEKLVAAGDKVKAGTPIAKFDATKIAEAGLDDTTMIIVTNTA
                     DFAEVSPLAEGTIAHGVEFLKVAK"
     gene            2214..3098
                     /gene="scrK"
                     /locus_tag="AC1PNG_000749"
     CDS             2214..3098
                     /gene="scrK"
                     /locus_tag="AC1PNG_000749"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HIP6701269.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFQVVEKTQFPTTTPYETIERT
                     VEFFKRYEDRLAGIAIGSFGPIDIDQNSQTYGYITSTPKPHWSNIDLLGLIAKEFNIP
                     FYFTTDVNSSAFGETLVRKGVKSLVYYTIGTGIGAGAIQNGEFIGGLGHTEAGHTYVA
                     LHPYDVKHEFKGMCPFHNGCLEGLAAGPSLEGRTGIRGELIELNSDVWDVQAYYIAQA
                     AVQATLLYRPQVIVFGGGVMAQEHMLNRVREKFVGLMNDYLPTPDVKEYIVTPAVAEN
                     GSATLGNFALAKQVSRNS"
     gene            3712..6465
                     /locus_tag="AC1PNG_000750"
     CDS             3712..6465
                     /locus_tag="AC1PNG_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIP6701270.1"
                     /translation="MIPIQQHGKRWLILTLTLSTSVLALASIGASQPVVFADENSHLQ
                     SQKNINKIEVLDWKTFSKKLSEYSSDQRQFHVLKLGFENRLGTLSTWDELEEFGKNNN
                     FLVINGKVTQNIHDFPHILVMNKGDVIAHNEEDYHNQMRELRFSGNGDLHNSMEPKRI
                     HALFKIELDSNKRQLLNAAGLGTAENSLKNINGMTIYSHGLTVDNKYYEDYSKYTHNS
                     VKNINVTKERFIANDDLIHKLIESSEAMKQSSERDKVKAFVQYVANHTTYDWEAANKA
                     VQNYADINYYLGSDLFAVTERQKAMCVGFSTTAARAFNMLGLPAYVVVGKNAEGVPHA
                     TARVYYDKKWHTIDGTGFITDITDSNKHQRSAKYSEKHFSTIGEDSYDVVEAGQEPKA
                     ERNYMIIDSNYESWAMKQKTADLLLFNKEKSLVGLDHIAYVEPTYITENKKNHLLDIY
                     KALKGKVEEAKATDTDDKDDKQEGYNRVLQFVNSDIDKLSTLSKITEEEFKALENSMD
                     LARVFLGQMNAKAGKEFSEGETYQSYLKNRQKNNTNSDDDRNRNELQENQANSDEVSQ
                     NSKDASAPSVNSAQHSEELEGTPSTQETISAAPSQQTPVAPKALQAKTELEDKTETSS
                     GSFERESNEVSANTVLSPEITKNTGSLASVESRVEEAVDRTTPSEKVSAESEEVEKTI
                     VSEVSLDASLANENKNSDAQEDTPRLEKSAEVVTTSQEVAEAPVSAPQVSSEIPTSPE
                     TNSTEAVTTSQEMAESRVSAPQVSFVSPTLEVPKNERLDEKADATMPNGVGANTHEAG
                     SVPTSDIYVQDAGSDTVPQPYSLSATLFEEAISTVEPVGVATSSQERSAVAGKVKVPA
                     SLERSNNSVEEEKVVDSNATIEKRATEKKEVLTSENVLNSLVTIWVALSTSFFMRYFS
                     RGK"
     gene            6785..9310
                     /gene="secA"
                     /locus_tag="AC1PNG_000751"
     CDS             6785..9310
                     /gene="secA"
                     /locus_tag="AC1PNG_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIP6701271.1"
                     /translation="MVTNVLRSLIENDKGELRKLEKMADKVFSYADEMEALTDEQLQA
                     KTAEFKERYNNGESLDDLLYEAYAVVREGARRVLGLYPYKVQVMGGIVLHNGDVPEMR
                     TGEGKTLTATMPVYLNALSGQGVHVVTVNEYLSTRDATEMGELYSWLGLSVGINLAAK
                     SPLEKREAYNCDITYSTNSEIGFDYLRDNMVVRAEDMVQRPLNYALVDEVDSILIDEA
                     RTPLIVSGAQGSETNQLYFLADNLVKSLTTEDYIIDIPSKTIGLSDSGIDKAEKFFKL
                     DNLYDIENVAITHFLDNALRANYIMTYDIDYLVNEDQEVMIIDPFTGRTMEGRRYSDG
                     LHQAIEAKEGVPVQNESKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVV
                     PIPTNRPIARVDHEDLLYPSLEYKFNAVIADVKRRYEKGQPVLVGTVAVETSDLISQK
                     LVAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGPGVRELGGLC
                     VIGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERIKVFMERMNLT
                     EEESVIKSKMLTRQVESAQKRVEGNNYDSRKQVLQYDDVMREQREIIYRQRQDVITAD
                     RDLAPEIKAMMKRTIERQVAGHFLGSKDEAIDGIIKFAHANLVEDDTLSKATFEAMNQ
                     KEIVEELYERALRVYDSQVKKLRDEERVREFQKVLILRVVDNKWTDHIDALDQLRNAV
                     SLRGYAQNNPIVEYQSEAFTMFNDMIGAIEFEVTRLMMKAQIHDNIERERTSQEAHTT
                     AVKNIMPNQSHAIQENVSFEGVDRNDPCPCQSGKKFKNCHGRK"
     gene            9425..9784
                     /gene="acpS"
                     /locus_tag="AC1PNG_000752"
     CDS             9425..9784
                     /gene="acpS"
                     /locus_tag="AC1PNG_000752"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIP6701272.1"
                     /translation="MIVGHGIDLQEMEAIESARIKHQGFPKKILTEKEFERYQSLSGR
                     RQLEYLAGRWSVKEALTKALGTGIGKIGFHDIEILNTSKGVPYVTKSPFDGNIWLSIS
                     HSGNFVQASVILEEKDD"
     gene            9777..10880
                     /gene="alr"
                     /locus_tag="AC1PNG_000753"
     CDS             9777..10880
                     /gene="alr"
                     /locus_tag="AC1PNG_000753"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIP6701273.1"
                     /translation="MIESEHRPTQIRVNLDAIAENFEQVMTNLPHKTEAFAVVKANAY
                     GHGAVAVAKKLSSQAAGFCVSNLDEALELRKAGIEHPILILGVVPVRFLPVAHQLNIS
                     VTVASLDWLQDAKDLEADLSGLTVHLKLDTGMGRIGFRKIADLLQAIAILEELEYDIE
                     GVYTHFATADEVDQAHFEYQLSIFKEFLDVLPITPRWIHASNSATSIWHADTVFNLVR
                     LGNILYGLNPSGRVLELPFDVQPALSLVSEIVHVKQVETGTTIGYGATYHSADSEWIA
                     TVPIGYADGLVRSLQGLSVLVDGQACEIVGRISMDQITIRLPHAYPLGQKVTLIGQDG
                     DKTISVQEWADCIGTINYEVVCLLTDRLPRIFE"
     gene            complement(11636..12991)
                     /locus_tag="AC1PNG_000754"
     CDS             complement(11636..12991)
                     /locus_tag="AC1PNG_000754"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIP6701274.1"
                     /translation="MKVIKFGGSSLASAEQLEKVFNIVQSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYKEYVNGKDVTASQEWIINRYQAMVDELGFTANSMKKIADSIISLAS
                     LPIDDNEFLYDAFLAAGEDNNAKLIAEYFTHRGLPARYVHPKKAGIIVSSEPGNARIL
                     PSSYDKIEELRDTDEVLIIPGFFGVTVDNQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIKNPHSIKELTYREMRELAYAGFSVLHDEALLPAYRGRIPLVIKN
                     TNNPTHPGTRIVHKHTEQTVPVVGIAADDGFVSINLSKYLMNREVGFGRKVLQILEDL
                     NIRWEHMPTGIDDLSIVVRERELTPIKEEEILRQLNTKMEVDKAEIEHGLSIIMIVGE
                     NMKSHVGVTATATTALSKQNVNLAMISQGASEVSVMFVVKTEEKKRALHALYEAFFNE
                     Q"
     gene            13168..13809
                     /locus_tag="AC1PNG_000755"
     CDS             13168..13809
                     /locus_tag="AC1PNG_000755"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIP6701275.1"
                     /translation="MVKAIIFDMDGVLFDTETFYFQRRADFLATKGLSVDHLDPSIFV
                     GGRASQIWQRILGDDYDNWDVPALEEEYRVYKEKRPTPYAERIFPEVKAVLERLTIQG
                     IPVVLASNTDRSEIERALLDAGIRFHFSHVFSAMDCEAPKPDPTVYMKAASATGVAKS
                     DILVFEDSAKGIEAAKKAGLTVWAIRDQHYGIDQSKADKLVDNLEEAFDLLRF"
BASE COUNT         4380 a         2561 c         3108 g         4244 t
ORIGIN      
        1 atcaagtttt aacaatgttt tttgataaaa tttaaataaa atgtgataaa cgtttgacat
       61 attttgtaaa agtgatataa taaaaccatc gaaagtttga aaacactttc aaaaatatac
      121 aaaaaggaga tttttgtaaa tggattataa aaaagtttct gccgaagtga tcgaagcggt
      181 tgggaaagat aatctagttg cggcagcaca ctgtgctact cgtcttcgtt tggttctgaa
      241 ggatgaatcc aaagtgaacc aaaaagccct cgatgataat gcagatgtca aagggacatt
      301 cagtacgaat ggtcagtatc aagttatcat cggacctggt gatgtaaact ttgtatatgc
      361 agagctcatc aaggctacag gcttaaaaga agtttcaaca gatgatttga aacagattgc
      421 agcgcaagat aagaagttca atccattgat ggacttgatc aagctcttgt cagatatctt
      481 tgtgccaatc attccggctc tcgttgctgg tggtctattg atggcgcttc gtaacttctt
      541 gacttcccca gatttatttg gtccaaaatc aattgaggaa atgtatcctg ctatccaagg
      601 catttctgcg atgattcaat tgatgtcagc agctccattc atgttcttgc cagtccttgt
      661 aggtatctcc gctgcgaaac gctttggtgc aaaccaattc ctcggtgcgg ctattggtat
      721 gatcatgacc actccagatt tgggtggtaa agaagctttt tgggatattt ttggtttcca
      781 agtagctcaa acaaactatg catatcaggt tattccggtt cttgcagcag tatggcttct
      841 tgcgacgctt gaaaaattct tccacaagaa gttgccatca gcagttgact ttacattcac
      901 accattattg tcagtaatga ttacaggttt cttgacattt acagtcatcg gtcctgtgat
      961 gttaaccgta tctgatgcga ttactaacgc aattgtttgg ttgtacgata caactggtgc
     1021 gttgggtatg agtatctttg gtggtactta ctcattgatc gtaatgactg gtcttcacca
     1081 atcattccca gccatcgaga cacaactttt gtctgcttac aacaataatg gtacaggata
     1141 cggagactac atctttgtag ttgcttcaat ggctaacgtt gcacaaggtg ctgcaactct
     1201 tgctatctat ttcttgacaa aaaatgcaaa aacaaaaggt ttgtccggtt cagcagcagt
     1261 ttctgccttc cttggaatta cagaaccggc cctcttcggg gttaacttga agtacagatt
     1321 cccattcttt tgtgctcttg caggttcggc tatcggtgcc ctctttgcag gtttgactca
     1381 tgttatcgct gtatctcttg gggcagcagg tttcattgga ttcctctcaa tcaaagcatc
     1441 ttctatccca atgtatgtag ttgctgaggt gattgctttt gctgctgcct ttgcctttac
     1501 ttacgtttat ggcaagacaa aagcatcagc agttttcgct gatgaagcag caacagcagc
     1561 tgcggcagca gtgacagcgt ctgtagttga aacttcagaa gaagttgaag aaactgcaga
     1621 aatctttact gcacaagaaa ctcttgtatc tccactttca ggtgacgttg ttgcccttga
     1681 aaatgttaat gacccagtct tctcatcagg agctatgggt aaaggtttgg ctgttaaacc
     1741 gtctgagggt gttgtttacg ctccagcaga tgcagaagta acaattgctt ttgaaacagg
     1801 ccatgcttac ggattgaaaa cagcgtctgg tgctgaactt ttaattcaca tcggtattga
     1861 tactgtatca atgaacggta atggatttga aaaattggtg gcagcaggtg ataaagtaaa
     1921 agctggtact ccgattgcta aatttgatgc aactaagatt gcagaagcag gtcttgatga
     1981 tacaaccatg atcatcgtga ccaacacagc tgactttgca gaagtatctc cacttgcaga
     2041 gggaacaatc gctcacggtg tagaattctt gaaagtagct aagtaatctt atataaaatc
     2101 gatttgtaga gtctgcctct actacataat taacaggaaa aaggctttca gcctttttct
     2161 tctgtttgct tatatgttat aataaaagtg ataaaaattt tggagacaat caaatgacaa
     2221 aattatatgg tagtttagaa gctggtggta caaagtttgt ttgtgcagta ggtgatgaga
     2281 actttcaagt tgttgaaaaa acgcaatttc caacgacaac tccttatgaa acgattgaga
     2341 gaacagttga attcttcaag cgctatgaag atcgtttggc aggtattgcg ataggttctt
     2401 tcggtccaat tgatattgat caaaactcac agacttatgg ttacatcact tcgacaccaa
     2461 aaccacactg gtcaaatatt gatttacttg gtctgattgc taaggaattt aacattccat
     2521 tctacttcac gacagatgtg aattcatcag cttttggtga aaccctggtt cgtaaagggg
     2581 taaagagctt ggtttactat acaatcggta caggtattgg tgcaggtgct attcagaatg
     2641 gtgagtttat cggtggcttg ggtcataccg aagcgggtca tacttacgtt gccctacacc
     2701 cttacgatgt aaaacatgaa tttaaaggga tgtgtccatt ccacaatggg tgtttggaag
     2761 gcttagctgc aggtccttct cttgaaggcc gtacaggtat tcgtggtgaa ttaatcgagt
     2821 taaattcaga cgtttgggat gtgcaggctt actacattgc tcaagcagca gtacaggcaa
     2881 ccttgcttta tcgtccgcaa gtcattgtat ttggtggagg agttatggct caagagcaca
     2941 tgcttaatcg tgtacgtgag aagtttgtcg gtttgatgaa cgattatctc ccaacaccag
     3001 atgtcaaaga atatatcgta acgccagctg ttgctgaaaa tggttctgca actcttggga
     3061 attttgcttt ggcaaaacag gtgtcaagaa attcataatt gtttatgtga acagattcat
     3121 ttgtactttg gcgatagata tattgaagaa gaatattggg tgaagttgtt ttattatcac
     3181 aataacttga cagtgaggtg agagttttta tgtaactctc acctcttttt acctaaaaaa
     3241 ctgtattcat aagagatagc cgtttgttaa tcaaggcagt ctttgggagt aggaaagaat
     3301 cagaccggct taaaaagagt tcgtcaacaa ctctttaatt taagtttttg agccgaaaca
     3361 gcgcctcaaa aaactaacga tgactataaa agattagagt ttgccattgt tggagttcat
     3421 cgggtatgaa aaagagaaac gagttccaca acttttgatg attattcata agcaaggttg
     3481 aaaatctttt tattggcgat agtgtagtga tattttttaa ccagaggaaa gttgttgttt
     3541 ctcaattgta tgaaaacgct atatctaaag taatgatgcg gagacactaa agtctataaa
     3601 aacaggaaaa gcacagatag atagcagaaa aaagatagtt aaaagtgtta caattgggat
     3661 gcaaatttta ggctgggaaa ttattcttag aagtatttat atggaggact tatgattcca
     3721 attcaacagc atggtaagcg ctggcttatt ttgacactta ccctttctac ttcagttctt
     3781 gcgcttgcat ctatcggagc atcacaaccg gttgtttttg cagatgaaaa ctcacattta
     3841 caatcacaaa aaaacattaa taaaatagag gttctggact ggaaaacatt ttctaaaaaa
     3901 ttgagcgaat actcctcgga ccaacgacaa tttcatgttt tgaaattggg atttgaaaat
     3961 cgtcttggga cattatcaac ttgggatgaa ttggaagaat ttggtaaaaa caacaatttt
     4021 ttggtaatca atggtaaagt tactcaaaat attcatgact ttccacatat ccttgttatg
     4081 aacaagggtg atgttattgc tcataatgaa gaagattatc ataatcagat gagagagttg
     4141 aggttttcgg gaaatggcga cttacataat agtatggagc caaaacggat tcatgcacta
     4201 tttaagattg aacttgactc caataaacgg cagttattaa atgctgcggg tctaggaact
     4261 gctgagaata gtttgaaaaa tattaatgga atgactattt actcgcatgg tcttacggtt
     4321 gataataagt actatgagga ttatagtaaa tatactcata attcagttaa aaatataaac
     4381 gttaccaaag agcgttttat agcaaatgat gacttgattc ataagttgat agagtcctca
     4441 gaagctatga agcaatcaag tgaacgcgat aaagttaagg catttgttca gtatgttgcc
     4501 aatcatacaa catatgattg ggaagctgct aataaggctg ttcaaaatta tgcagacatc
     4561 aattactact taggatcaga tttgttcgcg gttactgaac gtcagaaagc gatgtgtgta
     4621 ggtttcagca ctactgcggc ccgtgcattt aatatgttag ggcttcctgc ttatgttgtt
     4681 gttgggaaaa atgcagaagg agtcccgcat gctacagcgc gtgtttatta tgataaaaag
     4741 tggcacacca ttgacggtac aggttttatt acagatatta ctgattctaa taagcatcag
     4801 cgctcagcta aatatagtga aaaacacttt tctactatcg gtgaagatag ttatgatgtt
     4861 gttgaggcag gacaggagcc taaggcggag cgtaactaca tgattattga tagtaattat
     4921 gagagttggg ctatgaaaca aaaaacagca gacttactgt tgttcaacaa agaaaagtcc
     4981 ttggttggtt tggatcatat tgcatatgtt gagccaactt atataacaga aaataagaaa
     5041 aatcatttgc tagacatcta taaagctttg aaaggtaagg tagaagaagc gaaagcaacg
     5101 gatacagatg ataaagatga taaacaagag gggtataatc gtgttttaca atttgtcaat
     5161 tctgatattg ataaattgag tactctctcc aagattacgg aagaagaatt taaagcactc
     5221 gaaaattcaa tggatttagc tcgagtgttc ttggggcaaa tgaatgcgaa ggctggaaaa
     5281 gaattttcgg aaggagaaac ttatcaaagt tatctaaaga atcgtcagaa aaataacaca
     5341 aattctgatg atgatagaaa ccgaaatgaa ttgcaagaaa atcaagctaa ttctgatgaa
     5401 gtcagccaaa attccaagga tgcttcagct ccaagtgtta attctgcaca gcactctgag
     5461 gagcttgaag gaactccttc aacacaggaa acaatctcag ctgctccttc tcaacaaaca
     5521 ccagtagctc ctaaggcact acaagctaaa actgagttag aggataaaac agagacttca
     5581 tctggttcct ttgagagaga atcgaatgaa gtgtcggcta atacagtttt gtctcctgaa
     5641 ataactaaga acacgggaag tctagcttct gtagaatctc gagtggagga ggctgtggat
     5701 aggacaactc catcagaaaa agtttctgct gaatcagaag aagtagaaaa aacaattgta
     5761 tctgaggtat cattggatgc ttcattagca aatgagaaca aaaattctga tgctcaagaa
     5821 gatactccac gtttggagaa atctgccgag gtggtaacaa ctagtcaaga agtagctgaa
     5881 gcaccggttt ctgctccaca agtaagttct gaaatcccaa cttcaccgga aactaattct
     5941 acagaggcag taacaactag tcaagaaatg gctgaatcac gggtttctgc tccacaagtg
     6001 agttttgtat cgcctacttt agaggttcct aaaaatgagc gtttagacga gaaagcagat
     6061 gcaactatgc caaatggagt tggagcaaat actcatgagg caggttctgt tccgacatca
     6121 gatatttatg ttcaagatgc tggttcagac acggtgcctc aaccttactc tttatctgcg
     6181 acattgtttg aagaagcaat ttcaacagtt gagcctgtag gtgttgcaac ttcttctcaa
     6241 gagagaagtg cagtagctgg caaggtgaaa gtgcctgcct ctcttgaacg tagtaacaat
     6301 tctgttgagg aggaaaaagt agttgattcg aatgcaacaa ttgagaaacg agcgacagag
     6361 aaaaaagagg tccttacttc ggaaaatgta cttaatagct tagtaacaat ttgggtagct
     6421 ttatctacta gtttcttcat gagatatttt tctcgaggaa aataatatat taacacaggt
     6481 tgaagataat atttatcttt gtgtagaaaa ttattcatgt gagcttttat taaccgaata
     6541 atatgacaac tgagctttta atgaatttct aaatctccga tatatcgcag attcctcatt
     6601 taaaatttta taaatttttc acctcctagg agttattgtg actaggaggt gttttgtggt
     6661 aaaaatagta atattaagct agcattttca cttatttagg ctgtatccta gtgccaaacc
     6721 ttgaaaattg cctagttttc tgctacaata tattattgac taaaactgaa ataggatgaa
     6781 tgatatggta acaaatgtac ttcgttctct cattgaaaat gataagggag agctaagaaa
     6841 acttgagaaa atggcagaca aggtcttctc gtatgccgat gaaatggaag cgctgactga
     6901 tgaacagttg caagctaaga cagcagaatt taaggagcga tacaataacg gtgaatccct
     6961 cgacgatctt ttgtacgaag cttatgcggt agtccgtgaa ggagctcgtc gcgtattggg
     7021 tctctatccc tacaaggttc aggttatggg aggaatcgtt cttcacaacg gtgacgttcc
     7081 ggaaatgcgt acaggtgaag gtaaaactct gactgcaacg atgcctgttt atttgaatgc
     7141 cttgtctggt caaggtgttc acgtagtgac ggttaacgag tatctttcta ctcgtgatgc
     7201 gactgaaatg ggtgaattgt attcgtggct tggtctatca gttggtatca acttagctgc
     7261 taaatcaccc ttggaaaaac gtgaggccta taactgcgat attacttatt cgaccaactc
     7321 agagattggt tttgactatc tccgagacaa tatggttgtt cgcgcagaag acatggttca
     7381 acgtcctctc aattatgcct tggtcgatga agttgactca atcttgattg acgaagctcg
     7441 tacgccgttg attgtatctg gtgcacaagg ttcggaaaca aaccagttgt atttcttggc
     7501 agataatctt gttaaatctt tgacaacaga agattacatc attgacattc catctaaaac
     7561 gattggtctg tctgattcag gtattgacaa agcagagaaa ttcttcaagt tagataacct
     7621 ctacgatatt gaaaatgtag ccattaccca tttcttggat aatgcacttc gtgccaacta
     7681 catcatgacc tatgatattg actatctggt caatgaagac caagaggtca tgattatcga
     7741 tccatttaca ggtcgtacga tggaaggtcg tcgttattct gatggacttc accaagccat
     7801 cgaagctaag gaaggtgtac cggttcaaaa cgagtctaag acaagtgcct ctattaccta
     7861 ccaaaacctt ttccgtatgt ataaaaaatt atcagggatg acgggtactg gtaaaacaga
     7921 agaggaagaa ttccgtgaaa tctacaacat ccgtgtcgta ccaatcccaa ccaaccgccc
     7981 aatcgcgcgt gttgaccacg aagatctact ctatccaagt ctagaataca aatttaatgc
     8041 cgttattgct gatgtgaaga gacgctatga gaaagggcaa cctgtcttgg tcggtacagt
     8101 tgcggttgaa acttctgatt tgatttctca aaaattggta gctgcaggtg tccctcacga
     8161 agtattaaat gcaaaaaacc actatcgtga agcgcagatt attatgaatg ctggtcagcg
     8221 tggtgcggtc actattgcga ccaacatggc gggacgcggt accgatatta agttaggtcc
     8281 aggtgtacgt gaacttggtg ggctgtgtgt tattggtact gagcgccatg aaagtcgtcg
     8341 tattgataac cagcttcgtg gacgttcagg tcgtcaaggt gacccaggtg aatcacaatt
     8401 ctatctatct ctcgaagatg acttgatgaa acgctttggt tcagaacgca tcaaagtatt
     8461 tatggagcgt atgaatctaa cagaagaaga gtccgtcatc aagtctaaaa tgttgacacg
     8521 tcaagttgaa tctgctcaga agcgtgtgga agggaacaac tacgactcac gtaaacaagt
     8581 ccttcaatat gacgatgtga tgcgtgagca acgtgaaatc atctatcgcc aacgccagga
     8641 tgttattact gctgaccgtg atttggctcc tgaaatcaaa gcgatgatga aacggacgat
     8701 tgaacgccaa gttgcgggtc acttccttgg ttcgaaagat gaagctattg atggaattat
     8761 caaatttgcg catgcaaatc tcgttgaaga tgataccttg agtaaagcta cttttgaagc
     8821 aatgaatcag aaagagattg tcgaagagct ctatgaacgt gctttgagag tctatgattc
     8881 gcaagtgaaa aaattacgcg atgaagaacg tgtacgcgaa ttccagaaag ttcttatcct
     8941 tcgtgttgtg gataacaaat ggacagacca tattgatgcc ttggatcaat tgcgcaatgc
     9001 agtcagtctt cgtggatacg ctcaaaacaa tccaatcgtt gaatatcaat cagaagcatt
     9061 tactatgttc aacgatatga ttggggctat cgagtttgaa gtaacccgct tgatgatgaa
     9121 ggcgcaaatt catgacaata ttgagcgtga gcgtacaagt caagaagcac atacgacagc
     9181 tgttaagaat attatgccga accaatcgca tgcaattcag gagaatgtat cttttgaagg
     9241 tgttgatcgt aacgatccat gcccatgcca gtctggtaag aaatttaaaa attgtcacgg
     9301 ccgtaaatag gaatgacgca tggactggac ggctgtccag tccttttgca atttttagac
     9361 aagttactag agtcagttcg tttattttaa taaatgaaat gactatgcaa aaggagaccg
     9421 attaatgata gtaggacatg gcattgacct tcaggagatg gaggcgattg aaagtgccag
     9481 aatcaaacac caagggtttc ccaaaaagat tctcacagag aaggaatttg aacggtatca
     9541 aagtctgtcc ggtcgccgtc agctagaata tttagctgga cgttggtcag tcaaagaagc
     9601 gctgaccaag gccttgggga caggtattgg taagatagga tttcatgata ttgaaattct
     9661 gaataccagc aaaggagttc cctatgtgac gaagagtccc tttgatggga atatttggct
     9721 cagcattagc cattcaggaa attttgtaca agctagtgtg attttggagg agaaagatga
     9781 ttgaaagtga acatcgaccc acgcaaattc gggttaactt agatgcgatt gcggaaaatt
     9841 tcgagcaagt aatgaccaac ttgccccata agaccgaagc atttgcagtt gttaaggcga
     9901 atgcttatgg tcatggagct gttgcggtag cgaaaaagtt atccagtcaa gcagctggtt
     9961 tttgtgtttc gaatttggat gaagcattag agttacgtaa agcgggtatc gagcatccta
    10021 tcttgatttt aggagtggtt cctgtacgtt ttttaccagt agctcatcaa ctcaatatca
    10081 gtgtgacagt tgctagtctg gactggttgc aagatgcaaa agacttagag gctgatttat
    10141 ctggtctaac tgtccatctt aagctagata caggtatggg gcggattggt tttagaaaaa
    10201 tagccgattt gctacaagcg attgctatcc ttgaagaatt agagtatgac atcgaaggag
    10261 tttatactca ttttgctact gcggatgagg ttgatcaggc gcacttcgag tatcaactgt
    10321 ctatttttaa agaatttttg gatgttctac caattactcc gcgctggatt catgccagca
    10381 attcagcaac aagcatttgg catgctgata cagtatttaa cctggttcgt ttgggaaaca
    10441 ttctttatgg acttaatcca agtggacgag tactagaact cccgtttgat gttcaaccag
    10501 ccttatctct cgtatctgaa attgttcatg taaagcaggt tgaaactggc acaacaattg
    10561 gctacggagc tacttatcac agcgccgatt cagaatggat tgcgaccgtt ccgataggat
    10621 atgcagatgg tctcgtacga agcttacaag gcttatcagt cctagtcgat gggcaggcct
    10681 gtgaaattgt agggcgtatt tcgatggacc agatcaccat tcgcttacct catgcttatc
    10741 cacttggtca aaaagtcact ctaatcggtc aagatggaga taaaacgatc tctgttcagg
    10801 agtgggctga ttgcatcgga accataaatt acgaggtggt ttgtcttttg acagatcgcc
    10861 tacctcgaat ttttgagtaa acgtgacgat aatccgcctc tttcactagg atagaaggct
    10921 aaaatattag agaggtattg cctagagatt gtctataata caaatagcgt catttagttg
    10981 acttttatta cttcgacaat cgaggaaata tcgtttccat atttccaact tcaaacactc
    11041 cactggattg tttgaactcg ccttgcctga ttttgtaaaa gtgacttgct ttgcaagcaa
    11101 tctgggggat acgaatagtc cagcggacta ttcgtacctg agcctgaaaa caaaaaagcg
    11161 aagtgcatca gctggtcacc tagaaataag aaagcattga gttatgcctg cggcttttga
    11221 tgtgaacatc gttcactttg tccccagtct ccaactatct cccatatagt tggagcgagt
    11281 gctaaaagta aactaaaaga ctataatgag tctggtcaaa aagtctaata ctcttcgaaa
    11341 atcaaaatta tccgttgtca acttgccttg atgaacttca gttctatctt cgacttcgtt
    11401 tcctaggcta cttttgattt tcattgagta taaaaaataa aaaaatagct tagaattgca
    11461 tcgtcagaaa cgttgattct aagctatttt tgtttattca aattttaatt gctactcaat
    11521 gaaaattaaa atcagactag ctccaatgga gcttgatact catcaaatca agtcaacaac
    11581 gtctggtttt gattttcgaa gagtatgaag agggaactga ccgacctagt tgtgattatt
    11641 gctcgttaaa aaaagcttca tagagtgcgt gcagagcgcg tttcttttct tcggttttga
    11701 caacgaacat aacagaaact tcactggctc cctgtgaaat cattgccaag ttgacatttt
    11761 gtttagagag ggcagtggta gcggtagctg ttacaccgac atggctcttc atattttcac
    11821 caacaatcat gataatagat aaaccatgtt cgatttcggc cttgtcaact tccattttcg
    11881 tattgagttg acgaaggatc tcttcttctt taataggagt caattcacgc tcacggacta
    11941 cgatggataa atcgtcaata ccggtcggca tgtgctccca gcggatattg agatcttcga
    12001 gaatttgcaa caccttgcga ccaaaaccaa cttcgcggtt catcaagtat ttagacaagt
    12061 taatgctgac aaaaccatcg tctgcagcaa taccgacaac aggaactgtt tgctcggtat
    12121 gtttgtgtac aattcgggta ccagggtgag ttgggttatt ggtgtttttg ataaccaatg
    12181 gaatccgccc gcgataggca ggaaggaggg cttcatcgtg gaggacggag aaacctgcat
    12241 aagctaattc tcgcatttca cgataggtca attctttgat tgaatgtgga tttttaatga
    12301 taccaggatg ggcagcaaaa ataccatcga catctgtaaa gttttcatag aggtcagcct
    12361 tgactccggc agcaatgatg gaacctgtaa tatccgatcc acctcgtgag aaggtacaaa
    12421 tctggttatc aacagttaca ccgaagaatc ctgggataat gagtacttca tctgtatcac
    12481 gaagctcttc gattttgtca taacttgaag gcaaaatgcg agcatttcct ggttcggagc
    12541 tgacaataat accagctttt ttaggatgga catagcgtgc gggcaatcct ctgtgggtaa
    12601 aatattcggc aatcaatttg gcattattat cttcaccagc tgctaagaat gcgtcgtaaa
    12661 ggaattcgtt atcatcaatt ggtaggctgg ccaaagagat gatgctgtct gcgattttct
    12721 tcatgctgtt agctgtgaaa ccaagttcgt caaccattgc ctgataacgg ttgatgatcc
    12781 attcttgact ggcagtcaca tcttttccgt taacatattc tttataatat ttaatcaatg
    12841 catcagtaac tttagtatcc tcggcattgc gttttccagg tgcagacacc acaacaaagc
    12901 gacgttctga atcagattga acaatattga aaactttttc taactgttca gctgaagcaa
    12961 gtgagcttcc cccaaattta ataactttca ttctcatctc cttggatatt tttttctatt
    13021 atagcagaat gatatgaaat tgtcagtatg ttcagacaaa tcttgccaag aattttatcg
    13081 aaaaacgaac aagagacaag tatgaaaatt tcagaaaaac atttttttct ttcaactgtg
    13141 ttacaattaa agtaatagga ggcgagtatg gtaaaagcaa ttattttcga catggatggt
    13201 gtactttttg atacagaaac attctatttt caacgtcgag cggatttttt agcgacaaaa
    13261 ggtttatcgg ttgatcattt ggatccatct atatttgtcg gtggaagggc tagtcaaatt
    13321 tggcaacgga ttttagggga tgattatgat aactgggatg tccctgcctt ggaagaggaa
    13381 tatcgtgtat ataaagaaaa gcgaccaacc ccgtatgcag agaggatttt tcctgaagtt
    13441 aaagcggtct tggagagact aactatccaa ggtattcctg tagttttggc ttcaaatacc
    13501 gaccgttcag aaatcgaacg tgctctacta gatgctggta tacgctttca ctttagtcat
    13561 gttttctcag cgatggattg tgaggcccca aaaccagatc ccacagttta tatgaaagca
    13621 gctagtgcaa caggagtagc taaatcggat attttagtat ttgaagatag tgcgaagggg
    13681 attgaagcgg ctaaaaaagc aggtttgaca gtttgggcta ttcgagacca gcattatgga
    13741 attgatcagt cgaaagcaga caaattggta gacaatcttg aagaggcctt tgacttattg
    13801 cgattttaag aaatgttata ttttagtcca agtattagtt ctcggtgaaa aaatggtatt
    13861 tctagctcag aatccagtta gattttccaa aagtattaac gtgaaaaaat gaaagttgta
    13921 aattaaactt agagcctata tttacagttc agacttgcat attgaggttg ttttttcaaa
    13981 atacagtcag tatttcccgc aaaaactact ttgatgagtg aaaaagccaa ggcgctttgc
    14041 gtgtgaatat aggttcttag acacgttatt ttctagcata tctaaatttt atattgcgat
    14101 gatagccccc atctttttcg gacgtcacac tttacttgct agaaaaaaag tgacaattat
    14161 aaaaactgtg ttacaatgga aaacaagaat ttgaagtttg atattcaaat cttttaatta
    14221 tcaaactatt ttgaaaggag caagagaatg gcctaccaaa caattcgtta tgaagtaatt
    14281 gattccgtag cga
//
LOCUS       DBNFEG010000036        13908 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.036, whole genome shotgun sequence.
ACCESSION   DBNFEG010000036 DBNFEG010000000
VERSION     DBNFEG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13908
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.036"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..362
                     /gene="mscL"
                     /locus_tag="AC1PNG_000756"
     CDS             <1..362
                     /gene="mscL"
                     /locus_tag="AC1PNG_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIP6701276.1"
                     /translation="KDLKAFLFRGNIVDLAVAVVIGGAFGAIIKSFVNDIITPLILNP
                     ALKAANVQNIADLAWNGVKYGSFLSAVINFLIIGTSMFFVVKAAEKAMPKKVEEKAPA
                     GPTQEELLAEIRDLLKK"
     gene            complement(595..1608)
                     /gene="galE"
                     /locus_tag="AC1PNG_000757"
     CDS             complement(595..1608)
                     /gene="galE"
                     /locus_tag="AC1PNG_000757"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIP6701277.1"
                     /translation="MSILVTGGAGYIGSHTVVELLKLGKEVVIVDNLSNSSILVLDRI
                     ETITGKRPTFYELDVADKEALRQVFENENIEAAIHFAGYKAVGESVAKPIMYYENNIM
                     STLALVEVMTEFSVKKIVFSSSATVYGLNNPSPLVETMPTSATNPYGYTKVMLEQILR
                     DVEVADKEWSIALLRYFNPIGAHESGLIGEDPAGIPNNLMPFISQVAVGKREELSVFG
                     NDYDTIDGTGVRDYIHVIDLALGHIKALEKISTTTGVHTYNLGSGQGTSVLELVKAFE
                     KVNGVPVHYKLVDRRPGDVATCYANADKAWKELNWKTEKTIEDMCRDTWNWQSKNPNG
                     YEG"
     gene            complement(1712..1888)
                     /gene="rpsU"
                     /locus_tag="AC1PNG_000758"
     CDS             complement(1712..1888)
                     /gene="rpsU"
                     /locus_tag="AC1PNG_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIP6701278.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEAARKRKKF"
     gene            complement(1986..2615)
                     /locus_tag="AC1PNG_000759"
     CDS             complement(1986..2615)
                     /locus_tag="AC1PNG_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HIP6701279.1"
                     /translation="MRTKLQIIGTILFVLSAAVLGTIYLAWLVYPLEISFLGLEKVVY
                     MKVADISYNFNILMNYLTNPFASVLDMPNFSSSADGLKHFADVKHLFHLTQGIFILTL
                     PAFVLFVKNILLKGYGDLVKKVIFWTMLTPMIIGLLGVLVGFDQFFALFHTVLFPGDS
                     TWLFDPAKDPVIYILPQEFFLHCFVLFFVLYEFFFWTILAWTGKKNRIG"
     gene            complement(2605..3378)
                     /locus_tag="AC1PNG_000760"
     CDS             complement(2605..3378)
                     /locus_tag="AC1PNG_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HIP6701280.1"
                     /translation="MTYTGYLIDLDGTIYEGKKRIPAGERFIHRLQECQIPYLFVTNN
                     TTRRPEMVQAMLAENFNIETPLETIYTASLATVDYMNDLGKEKTVYVIGEDGLKSAIF
                     EAGYVEDTENPAYVVVGLDTQLTYEKLTIATLAIQKGATFIGTNPDLNIPTERGHLPG
                     AGSLIALLKAATRVEPTFIGKPEVIIMDKALEILGTERSQTVMVGDNYLTDIRAGIDN
                     GFPTLLVLTGFTKPEEVADLPLAPTHVLNSLDEWSFDEN"
     gene            complement(3388..4005)
                     /locus_tag="AC1PNG_000761"
     CDS             complement(3388..4005)
                     /locus_tag="AC1PNG_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HIP6701281.1"
                     /translation="MSQDKWLEWAVRLQALAQTGLAYGKDVYDMERFEEIRQIAAEML
                     VEPSGQPLEVVKDLFCNETGYQTPKLDTRAAIFQEDKILLVQENDGLWSLPGGWCDVD
                     QSVKDNVVKEVKEEAGLDVEAQRVVAILDKHKNNPAKSAHRVTKVFILCRLLGGEFQP
                     NSETVASGFFSLDDLPPLYLGKNTAEQLALCLEASRSEHWETRFD"
     gene            complement(3992..4732)
                     /locus_tag="AC1PNG_000762"
     CDS             complement(3992..4732)
                     /locus_tag="AC1PNG_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HIP6701282.1"
                     /translation="MGLTYQEEFTIPFDMVDVKQEIKLPDFISYCLGVSGRQSEELGR
                     SDLYVFQEFGLIWVVTDYELTIQRLPKYNETITIKTEAVAYNKFFCHRMFYIYDAAGN
                     LLLDILCYFVLIDFETRKVAPVPEDLIAPYQSEQVKKLPRAPKYQLLENPSVQEFPVR
                     YFDLDMNGHVNNGKYLEWMYEALGYDFLLCHVPKKIQLKYLKEVEATSLVSSRMVAEA
                     GGSQHEIVVDGHIHAQAVIEWRERHVAG"
     gene            complement(4963..6090)
                     /gene="hemW"
                     /locus_tag="AC1PNG_000763"
     CDS             complement(4963..6090)
                     /gene="hemW"
                     /locus_tag="AC1PNG_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIP6701283.1"
                     /translation="MKKRPTSAYIHIPFCTQICYYCDFSKVFIKNQPVDEYLAALMEE
                     VKFYDLPALRTLYIGGGTPSALSADQLDYLLTNLEDLLDLSQLEEFTIEANPGDLTAD
                     KIAVLKKSKCNRVSLGVQTFDDRMLKKIGRSHNQAQIYETIATLKEAGFYNISIDLIY
                     ALPGQTMEQVVDNVAKALELDIPHMSLYSLILENHTVFMNRQRRGNLHLPNEDVESDM
                     FDYILQELEKNGFEHYEISNFTKPGFESRHNLMYWDNSEYYGLGAGASGYIDGMRYRN
                     RGPIQHYLKSIREKGHSRLHEEFLSQTEQMEEEMFLGLRKKTGVSIERFEEKFGISFE
                     DRYGQVVRDLKNEGLLQEEDRWLRMTKKGLFLGDTVAERFI"
     gene            6185..6763
                     /locus_tag="AC1PNG_000764"
     CDS             6185..6763
                     /locus_tag="AC1PNG_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIP6701284.1"
                     /translation="MKKEIIYDCINLILSFLLCRWLFMTFLFYQFSNIFMTIDFWPTL
                     AAILLMTGFCFTLFRLVYKPHISRLTLWFFYACYGLLLVYLLFFKSMGVRGVNWNLLS
                     TFSQDLFLNPTILVFNLLLFLPLGLLFSFSWKKLSLFVGAILLVEACQFFFSLGFFDL
                     GDILLNTSGFALGNFLGQSAIAQSFKNRIQKK"
     gene            complement(6898..9165)
                     /gene="glgP"
                     /locus_tag="AC1PNG_000765"
     CDS             complement(6898..9165)
                     /gene="glgP"
                     /locus_tag="AC1PNG_000765"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HIP6701285.1"
                     /translation="MINLQNFVQSMYAKRIEDCTDQELYYALLAFTKQQSEAKYTNDQ
                     KKKVYYISAEFLIGKLLSNNLINLGLYDEVKSQLVAAGKDLIAIEDMELEPSLGNGGL
                     GRLAACFLDSIASLGLNGDGVGLNYHFGLFRQLFEYHQQSAVPNEWITPRSWLTESPI
                     SYQVPFANFTLTSKLYDIDVPGYKMERKNRLRLFDLGSVDDNIIYDGIEFDKEDIFRN
                     LTLFLYPDDSTDEGKVLRIFQQYFMVSNAAQLLIDEAVAKGSNLHDLPDYAVVQINDT
                     HPSLVIPELIRLLELRGISFDEAVEIVKKMTAYTNHTILSEALEKWPLDFLNYVVPHL
                     VPFIVELDRRAKEVKDDPAVNIIDEYGRVHMAHMDIHYGYSINGVAALHTEILKTSEL
                     KAFYELYPEKFNNKTNGITFRRWLMHANPSLASYLDGLLGHGYHHDASELEKLLDYKN
                     DKELKSWLEKTKQHNKRKLQRHIAKTQGVHVNPESIFDVQIKRMHEYKRQQMNVLYVI
                     HKYLDIKAGNIPTRPLTVFFGGKAAPAYTTAQDIIHLILVLSQVIKNDSEVAPHLQLV
                     MIENYNVTEASFIIPAADISEQISLASKEASGTGNMKFMLNGALTIGTDDGANVEIHE
                     LVGDENIYIFGQDSQTVIEHYANRTYHPYSFYEREVIRPLIDFITSDTIRHAGGIDER
                     LWRLQDNLKHNDHFMTLLDLEDYIVTKERMLADYEDRDSWLEKVIVNIAKAGFFSSDR
                     TIQQYNEDIWHLEAK"
     gene            complement(9342..10055)
                     /gene="deoD"
                     /locus_tag="AC1PNG_000766"
     CDS             complement(9342..10055)
                     /gene="deoD"
                     /locus_tag="AC1PNG_000766"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIP6701286.1"
                     /translation="MSIHISAKPGEIADKILLPGDPLRAKFIAENFLEDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYAHELINEYGVKKLIRVGTAGSLNEDVHVRE
                     LVLAQAAATNSRMINIDWPEYDLPQIADFDLLDKAYHIAKELNMTTHVGNVLSSDSFY
                     SPKLFSRNLELGQLGVKAVEMEAAALYYLGAKFGVQTLAIMTISDSLVSPEENTTAEE
                     RQNTFTDMMKVGLETLIAE"
     gene            complement(10088..10897)
                     /locus_tag="AC1PNG_000767"
     CDS             complement(10088..10897)
                     /locus_tag="AC1PNG_000767"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIP6701287.1"
                     /translation="MSLLAKINETKTFLEEKGLVKPEFGLILGSGLGELAQEVENAIV
                     IDYADIPNWGKSTVVGHAGKLVYGDLAGRKVLALQGRFHFYEGNPMEVVTFPVRVMKA
                     LGCEGVIVTNAAGGIGYGPGTLMAITDHINMTGQNPLIGENLEEFGPRFPDMSNAYTK
                     EYREKAHAVAEKLGIKLDDGVYLGVTGPTYETPAEILAFKTMGAHAVGMSTVPEVIVA
                     AHSGMKVLGISAITNFAAGFQSELNHEEVVEVTEQIKGNFKGLVKAILAEL"
     gene            complement(10909..11265)
                     /locus_tag="AC1PNG_000768"
     CDS             complement(10909..11265)
                     /locus_tag="AC1PNG_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HIP6701288.1"
                     /translation="MEKLTVYYNPNCSKCKKLRILLSSQDMEVKWVNYLETPLRGAEL
                     TALLEKMGSQPSAVTRLKEEERLALSEEEIFERLVEEPATLNRPIIEREQTAFLCRPL
                     EIIKEKMPEYDWSDYL"
     gene            complement(11265..12476)
                     /locus_tag="AC1PNG_000769"
     CDS             complement(11265..12476)
                     /locus_tag="AC1PNG_000769"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIP6701289.1"
                     /translation="MPKFKRVHLVVMDSVGIGAAPDSDKFFNAGVADTESDTLGHISE
                     KAGLEVPNMTKIGLGNIPRDTALATVPAESHPTGYVTKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWDGFPEEILTKIEEFSGRKIIREANKPYSGTAVIDDFGPRQMETGELIV
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFSRT
                     ANRHDYAVSPFKATVLNKLADAGVPTYSVGKISDIFNGSGITNDMGHTKSNMHGVDVL
                     IDTLKLSDFEEGFSFTNLVDFDAVYGHRRNIEGYRDCLQEFDARIPEIIDNMREDDLL
                     LITADHGNDPSYAGTDHTREYVPLLAYSKAFKGSGVLPVGHFADISATVAENFGVDTA
                     MIGESFLSQLV"
     gene            complement(12805..13479)
                     /gene="rpiA"
                     /locus_tag="AC1PNG_000770"
     CDS             complement(12805..13479)
                     /gene="rpiA"
                     /locus_tag="AC1PNG_000770"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HIP6701290.1"
                     /translation="MTNLKEQVGIKAAEFVTDGMIVGLGTGSTAYYFVQEIGRRVAEE
                     GLQITGVTTSHATAEHAASLGIPLKNIDEVEYVDLTVDGADEVDGDFNGIKGGGAALL
                     MEKVVAVNSKDCIWIVDESKVVQTLGAFKLPVEVVQYGAENLFRLFEKKGYRPSFRMR
                     NGEKHITDMQNFIIDLDLRRIEDTYALAEELDRTVGVVEHGLFIGLISKVIVGTLEGP
                     NIIEKK"
BASE COUNT         3960 a         3158 c         2689 g         4101 t
ORIGIN      
        1 tgaaagattt aaaagcattt ttgttccgtg ggaacattgt tgaccttgct gtagcagtgg
       61 taattggtgg agcgttcggc gcaattatca aatcatttgt aaatgatatc atcactccat
      121 tgattttgaa cccagctttg aaagcggcaa atgtacaaaa tattgctgac cttgcatgga
      181 atggtgtgaa gtacggtagt ttcttgagcg ctgttattaa cttcctaatc attggcactt
      241 caatgttctt tgttgttaaa gcagctgaga aagcaatgcc taagaaagtt gaagagaaag
      301 ctcctgctgg tccaactcaa gaagagttgc ttgcagaaat ccgcgatttg ttgaaaaaat
      361 aatcaataat atattgggag tgggaaagaa ctcgaccagt ctaaaaagag ttcgtcttcc
      421 cacccccgca cagttgatta ggtcagattt ggagtatgaa acacgaacaa atctgccaat
      481 caaccactgc gctgagatgt tgacacgaac tctgagaagc gaggatggac tttctgccca
      541 gcctctcttc cataattaca aaaataagat gtgagcctcc actcacatct tttcttatcc
      601 ttcgtatcca tttggatttt tcgattgcca gttccatgtg tcgcggcaca tatcttcaat
      661 tgttttttct gttttccagt tcaactcttt ccaagccttg tctgcgttag cgtaacaggt
      721 tgccacatca cctggacgac ggtcaacaag cttataatgt actggaacgc catttacctt
      781 ttcaaaggct ttgaccaact ctaatacact tgttccttga cctgaaccaa gattgtaagt
      841 atgtacacct gtagtagttg aaattttttc aagcgccttg atatgaccga gtgccaaatc
      901 aatcacatgg atataatcac gcactccagt accatcaatt gtatcataat cgttaccaaa
      961 aacgctgagc tcctcacgtt taccaactgc tacctgtgaa ataaacggca tcaaattatt
     1021 aggaatacca gctggatctt ccccaatcaa gccagattca tgggcaccga ttggattgaa
     1081 ataacggagc aatgcaatac tccactcctt atctgctact tccacatcac gcaagatttg
     1141 ttccaacata actttggtat agccgtacgg attcgttgcg ctagttggca tagtttcaac
     1201 caaaggcgat ggattattga ggccataaac agtcgcactt gaggagaaga caattttctt
     1261 aacactaaat tctgtcatga cttccaccaa agctagggtt gacatgatat tgttttcata
     1321 gtacataatt ggcttagcca ctgattctcc tactgccttg tagccagcaa agtgaatggc
     1381 tgcctcaatg ttttcatttt caaaaacttg gcgcaaggct tccttatctg ccacgtccaa
     1441 ctcatagaaa gttgggcgct tgccagtgat ggtttcaatg cgatctaaaa ccaaaatact
     1501 tgagtttgaa agattatcca cgataaccac ttctttcccc aacttcaaca attcgacaac
     1561 cgtatgacta ccaatataac ctgcaccacc agtaactaag atactcatct ttttactcct
     1621 tttcacctag acaaaattct atatccctat tatatgttac ctgttgtaaa aagtaaacaa
     1681 aaaacagcct ttcggccgtt ttcaaattca attagaattt tttacgcttg cgagctgctt
     1741 ctgatttacg tttacgtttt acagatggtt tttcgtagaa ttcgcgtttg cgtgtttctt
     1801 gaagagtacc agctttagta accgcacgtt tgaaacgacg aagagcatca tcaagtgatt
     1861 cgttcttacg tactactgtt tttgacattt tttcactccc ctcaatttca aagtctttat
     1921 cattttacta tgattcaact catttgtcaa tatttttttc attctaaaat atgaatttta
     1981 cgttcttatc ctatcctatt cttttttcct gtccacgcca gtattgtcca aaagaagaat
     2041 tcgtaaagga caaaaaataa tacaaagcaa tgcaagaaaa actcttgggg caaaatatat
     2101 atgacaggat cctttgcagg gtcaaatagc catgtagaat ctcctggaaa tagcaccgta
     2161 tgaaacaatg caaaaaactg atcaaagcca acaagaactc ctaataaacc gattatcatt
     2221 ggcgtaagca tggtccagaa aatgaccttt ttaaccaagt ctccataacc ttttagcaga
     2281 atgtttttta caaaaagcac aaaggcaggg agggtgagga taaaaattcc ctgagttaga
     2341 tggaataaat gtttcacatc cgcaaaatgt ttcagaccat cagctgagga tgaaaaattt
     2401 ggcatgtcta aaacacttgc aaaaggattt gttagatagt tcatcaggat attgaaattg
     2461 taggaaatat ctgccacctt catgtagacc actttttcca accccaaaaa agaaatctca
     2521 agcggataaa ctagccaagc taggtagatg gttcctagaa ctgctgccga taagacaaat
     2581 aaaatcgttc cgataatctg taacttagtt ctcatcaaaa ctccattcat ctaggctgtt
     2641 gaggacatgg gttggagcca ggggtaagtc tgctacttct tctggcttgg taaatcctgt
     2701 caggactaaa agagttggaa atccattgtc aatccctgca cgaatatctg tcaggtagtt
     2761 atctcctacc ataactgtct ggctgcgttc tgtgcctaaa atctccagag ccttgtccat
     2821 gataatgact tcaggcttac cgataaaggt cggctctact cgggtagcag ctttcaaaag
     2881 cgcaataagt gagcctgctc ctggcaaatg acctctttca gtcggaatgt tcaaatcagg
     2941 attggtaccg ataaaggtcg ctcccttttg aatagctaag gtggcaatgg tcagtttttc
     3001 ataggtcaac tgagtatcca aaccgacaac aacataggct ggattttccg tgtcttccac
     3061 ataacctgcc tcaaaaatgg cagacttgag cccgtcttct ccaatgacat agactgtctt
     3121 ttccttgccc aagtcattca tataatctac tgtcgccaga ctggctgtat agattgtttc
     3181 aagcggtgtc tcaatgttaa agttttcagc caacatagcc tgcaccatct caggacggcg
     3241 ggtggtattg ttggtcacaa ataaataggg aatctgacat tcttgcaagc gatgaataaa
     3301 acgttcacca gcaggaatcc gctttttccc ttcataaatc gtaccatcca aatcaatcaa
     3361 ataacctgta tatgtcattt ttcctcctta atcaaatcgt gtttcccaat gctcagaccg
     3421 actagcttcc aagcaaagtg ctaactgctc agctgtattt ttcccaaggt agagcggtgg
     3481 taagtcatct agactgaaaa agccactggc aactgtttcc gaattgggct gaaactcccc
     3541 gccaaggaga cgacatagaa tgaagacctt ggtcacgcgg tgggctgact tggcaggatt
     3601 gttcttgtgt ttgtccaaaa tcgccaccac acgttgagcc tccacatcca gccccgcttc
     3661 ttcctttact tccttgacta cattgtcctt gacagactgg tccacatcgc accaaccacc
     3721 tggtaaggac cagagcccat cgttttcctg aaccagtagg attttatcct cttggaaaat
     3781 agctgcgcgg gtatccagct tgggtgtctg atagcctgtt tcattgcaaa acaagtcttt
     3841 cactacttcc aagggctgtc cagatggctc taccaacatt tccgccgcaa tctgtctgat
     3901 ttcctcaaat cgctccatgt cataaacatc ttttccatag gccaagcctg tctgagccaa
     3961 ggcttgtaaa cgtacggccc attccaacca cttatcctgc gacatgacgt tccctccatt
     4021 ctatgacagc ttgcgcatgg atatgcccat ctaccacaat ctcatgctga ctaccaccag
     4081 cttctgccac catacgcgaa ctcaccaagc tagttgcctc tacctcttta agatacttga
     4141 gttgtatttt tttagggaca tggcagagca gaaaatcata gcccagtgct tcatacatcc
     4201 attccaaata cttgccatta ttgacatgcc cattcatatc caagtcaaaa tagcgaacag
     4261 gaaattcttg gacagatgga ttttccaaga gctgatactt gggagcccga ggcaatttct
     4321 tgacctgctc agactgataa ggagcaatca aatcttctgg cacaggcgcc accttgcgag
     4381 tttcaaaatc aatcaggaca aaatagcaga gaatgtccaa caaaagattg cctgctgcat
     4441 cgtagatata gaacatccga tgacagaaaa acttgttgta ggcaaccgct tctgtcttaa
     4501 tggtaatggt ttcattgtac ttaggtaaac gttgaatggt cagttcataa tctgtcacca
     4561 cccaaatcaa gccaaattcc tgaaaaacat agaggtcact gcgacccagt tcttccgact
     4621 gccgacctga tacaccaaga cagtaggaaa taaagtcagg tagtttgatt tcttgtttga
     4681 catccaccat atcaaaagga atggtgaact cttcttggta tgttagtccc ataatgttga
     4741 tctttctagt cttaaagtat agtctttcag ttcacgtcaa cagctgcagg tagcttcaat
     4801 agtccagtgg actattgaag gttggaaatc gtacttacga tagtaagtaa cattcgtaga
     4861 gtacggcaaa gtgagttcaa atactccagt ggagtgtttg aagttggaaa taaggaaacg
     4921 aagtttcttc gctcgtcgaa gtaataaaaa caaactgaat gactatatga aacgctctgc
     4981 aactgtatcg cccagaaaca accccttttt agtcatccga agccaacggt cttcttcttg
     5041 caaaagacct tcatttttca agtctctgac cacctgaccg tagcggtctt caaaggaaat
     5101 gccaaacttt tcctcgaatc gctcaatgga cacgccagtt tttttccgta gccctaaaaa
     5161 catctcttct tccatctgct cggtctggct gaggaattct tcgtgtaagc gagaatggcc
     5221 tttttcgcgg atggacttga ggtagtgctg aatggggccg cggttgcgat agcgcatgcc
     5281 gtcgatgtag ccagatgctc cagctcctag accataatac tccgagttgt cccagtacat
     5341 gagattgtgg cggctttcaa agccgggctt ggtgaagttg gagatttcgt agtgctcaaa
     5401 gccgtttttc tccaactcct gaagaatgta gtcgaacata tcggactcca catcttcgtt
     5461 gggtagatga agattgccac ggcgttggcg gttcatgaag accgtatggt tctccaaaat
     5521 caagctatag aggctcatgt ggggaatgtc cagctccaag gccttggcga cattatcgac
     5581 cacctgctcc atggtctgac caggcagggc ataaatcagg tcgatggaaa tattgtaaaa
     5641 tcctgcttcc ttgagggtag caatggtttc ataaatctgg gcctgattgt ggctgcgacc
     5701 gatttttttc aacatgcggt cgtcaaaggt ctgaacgccc agcgataccc gattgcactt
     5761 ggattttttc agaactgcaa tcttgtctgc cgtcaagtca cctggattgg cctctatagt
     5821 gaattcttcc agctgtgaca agtccagcag gtcctccaga ttggtcagga gataatctag
     5881 ctggtctgcc gagagagctg acggtgttcc gcctccaata taaagtgtgc ggagagctgg
     5941 caggtcgtaa aacttgacct cttccataag agctgccaaa tactcgtcaa caggctgatt
     6001 cttaataaag accttggaaa aatcacagta gtaacaaatc tgggtacaaa atggaatgtg
     6061 gatataggca gatgtcggtc gttttttcat accaactatt atagcacact gtctataaaa
     6121 atattttcta cagccttgac ctcccctcaa ataatgatat aatgaaataa aaaggagatc
     6181 ctctatgaaa aaagagatta tctacgactg tattaacctg atcctgtcat ttctgctttg
     6241 ccgctggctc ttcatgacct ttctttttta ccaattttca aacattttca tgacgattga
     6301 tttttggccg acacttgccg caatcctgtt aatgaccgga ttttgcttta ccctatttcg
     6361 acttgtctat aaaccccaca tttcgcgact gacactttgg tttttctacg cttgctacgg
     6421 gcttctgcta gtctatcttc tctttttcaa aagcatgggc gtcagaggtg tcaactggaa
     6481 tctccttagc actttttctc aagacctatt tctcaatcct actatattgg tttttaacct
     6541 cttactcttt ttaccacttg ggttgctttt ttcattttct tggaaaaaac tttctctctt
     6601 tgtaggggcc atattgctag ttgaagcctg tcaattcttc ttttcactgg gcttctttga
     6661 ccttggtgac atcttgctta acacttctgg ctttgccctt ggaaacttct tgggacaatc
     6721 cgcaatcgct caatctttta aaaatagaat acagaaaaaa tgaccccgca aggtcatttt
     6781 tagtttattt atactcaatg aaaatcaaaa gtagcctagg aaacgaagcc gacgatagaa
     6841 ctgaagttca tctaggctct tgacaacgga taattttgat tttcgaagag tattagctta
     6901 cttagcttcc aaatgccaaa tatcttcatt gtactgttga atcgtacggt ctgatgagaa
     6961 gaaacctgct ttagcgatgt tgacgatgac tttttccaac cagctgtcgc ggtcttcata
     7021 gtcagccaac attctttcct tagtcacaat atagtcttcc aaatctagca aggtcatgaa
     7081 gtggtcattg tgcttgaggt tgtcctgcaa acgccagaga cgctcatcga tcccaccagc
     7141 atgacgaatg gtatctgatg taataaagtc aatcaatgga cgaatgactt cacgttcgta
     7201 gaaggaatat ggatggtagg tcctatttgc atagtgttcg ataactgttt ggctatcttg
     7261 accaaagata taaatatttt catcacctac caactcatga atttccacat tggccccatc
     7321 atctgtaccg atggtcaagg ctccattgag catgaatttc atgttacctg taccagatgc
     7381 ttccttagat gcaagtgaaa tttgctcaga aatatccgct gctggaatga taaagctagc
     7441 ttcggttacg ttgtagttct caatcataac tagctgcaaa tgtggtgcca cttctgaatc
     7501 atttttgata acctgtgata agaccagaat caagtggatg atgtcttgag ctgttgtata
     7561 ggctggagct gcttttcctc caaagaagac tgttaacggg cgagtcggga tattgcccgc
     7621 cttgatatcc aaatacttat ggataacata gaggacattc atctgttgac gcttgtactc
     7681 gtgcatgcgc ttgatttgta catcaaagat ggattcagga ttgacatgta caccttgagt
     7741 tttggcaata tgacgttgca acttccgctt gttgtgttgc tttgtctttt ccaaccaaga
     7801 tttcaattcc ttatcatttt tgtaatccaa cagcttttct agttcacttg catcgtgatg
     7861 gtagccatgt ccaagtaggc cgtccaggta gcttgccaaa cttggattag catgcatgag
     7921 ccagcgacgg aaggtaatcc catttgtctt attattgaat ttttcaggat atagctcata
     7981 gaaagctttc aactctgaag ttttcaaaat ctctgtatga agtgccgcca ccccattgat
     8041 agaataacca taatgaatat ccatgtgtgc catgtgaaca cggccatatt catcgatgat
     8101 attgacagca ggatcatcct tcacttcttt ggcacgacga tccaattcta cgatgaacgg
     8161 taccaagtgc ggaaccacat agttcagaaa atcaagcggc catttttcca gcgcctctga
     8221 caagatggtg tggttggtat aagcagtcat tttcttaaca atttcaactg cctcatcaaa
     8281 tgaaatacca cgaagctcca aaaggcgaat caattctgga ataacaagtg atgggtgagt
     8341 atcgttgatt tgaacaacag cataatcagg caggtcatgt aagtttgacc ccttagcaac
     8401 tgcttcgtca atcaatagtt gcgcagcgtt agacaccatg aaatactgct ggaagatgcg
     8461 gagaactttt ccttcatcgg tcgaatcatc tggatagagg aagagggtca agtttcggaa
     8521 aatatcttcc ttatcaaatt ctattccgtc ataaatgata ttgtcatcaa ctgaaccaag
     8581 gtcaaacaag cgaagacgat tcttacgctc cattttataa ccaggtacat cgatgtcata
     8641 caacttagat gtcaaggtaa agttagcaaa tggcacctga taagaaatcg gtgactctgt
     8701 taaccatgaa cgtggtgtaa tccactcgtt tggcacagca gactgctgat ggtattcaaa
     8761 taattgtcta aacagtccaa agtgataatt caagccgacc ccatcaccgt taagacctag
     8821 actagcaatt gaatcaagga agcaggcagc caagcgcccc aaacctccat taccaagaga
     8881 cggttcaagc tccatgtctt caatagcaat caaatcctta ccagccgcca ccaactggct
     8941 tttaacctca tcataaagac caaggttgat taaattatta gacaagagct taccaattaa
     9001 gaactctgct gaaatatagt agaccttttt cttttggtca tttgtatact tagcttcact
     9061 ctgctgtttt gtaaaagcaa gcaaggcata atagagttct tggtctgtac aatcttcaat
     9121 gcgtttggca tacatagact gaacaaaatt ttgtaaatta atcatgagga ctctcctaga
     9181 tatattaatg taatcattca gaaaggtttc gctttgcctt tgtaaacgtt ttcttaatta
     9241 taccctattt ataaaaaaaa agacaagcca acttccgtac aaatagaaat tagttcgtct
     9301 tttaaacgat tgcaggttac tacaatcaga attattctta attattctgc aatcagagtt
     9361 tccaaaccaa ctttcatcat atccgtgaaa gtattttgac gctcttctgc tgtggtgttt
     9421 tcctctggac taaccaagct atctgaaatc gtcataatgg caagcgtttg aacgccaaac
     9481 ttagcaccta ggtaataaag tgcagctgct tccatttcta cagccttgac acctagttga
     9541 cctaattcta agttgcgact aaacaatttt ggagaataga aactatctga tgataaaaca
     9601 ttgccaacat gcgttgtcat gttgagttct ttggcgatat gataagcctt atctaaaaga
     9661 tcaaaatcag cgatttgtgg caaatcgtac tctggccagt caatattaat catgcgagag
     9721 ttggtcgcgg ctgcttgcgc caaaaccaat tcacggacat ggacatcctc attcaaagaa
     9781 cctgctgttc ccacacgaat tagttttttc acaccatatt cgttaatcaa ttcatgagca
     9841 tagatagaga tggatggcat tcccatacct gttcccatga cagaaacacg gtgacctttg
     9901 taagtaccag tatagccaaa catattgcgg acttcattga agcaaactgc gtcttcaaga
     9961 aaattttcag cgataaattt agcacgaagc ggatcaccag gaagcaaaat cttatcagcg
    10021 atttcgcctg gttttgcgga aatatggata gacatgtaat gtacctcttt gttctatttt
    10081 cgaatgctta caactcagcc aagatagctt tcaccaagcc tttgaagttg cccttgattt
    10141 gctcggttac ttctacaact tcttcgtgat tgagttcaga ttggaaacca gctgcaaagt
    10201 ttgtgatagc agaaataccc aagactttca tacctgagtg agctgccacg ataacttctg
    10261 gtactgttga cataccgact gcgtgggcac ccatagtctt gaaggccaag atttcagcag
    10321 gtgtttcata ggtagggcct gtcacaccga gatagacacc atcgtccaac ttgataccca
    10381 atttttccgc aacggcatgg gctttttcac ggtattcttt agtgtaggca ttggacatat
    10441 ctgggaaacg tggaccaaat tcttccaagt tttcgccaat caatggattt tggccagtca
    10501 tgttgatgtg gtctgtaatg gccataaggg tacctggacc gtagccaata ccacctgcgg
    10561 cattagtaac aatcacgcct tcacaaccaa gagctttcat gacacggact gggaaagtca
    10621 cgacctccat aggattgcct tcgtagaagt ggaatcgacc ttgaagagct aagaccttac
    10681 gtcctgccaa atcaccgtac accaacttac cagcatgacc aacaactgtt gacttacccc
    10741 agtttggaat atctgcatag tcaatgacaa tggcattttc aacttcttgg gccaattcac
    10801 ccaagccaga acccaaaatc aaaccgaatt caggttttac caaacctttt tcttccaaga
    10861 acgtttttgt ttcattgatt tttgcaagta aactcatatt ttctcctttt ataaataatc
    10921 cgaccagtca tattctggca ttttttcttt aatgatttca agcggacgac agaggaaggc
    10981 tgtctgctct cgctcgataa tggggcgatt gagagtggca ggctcttcca ctaaacgctc
    11041 aaagatttct tcttctgaca aggctagtcg ttcttcttcc ttcaatcgag taactgctga
    11101 aggttgactc cccatttttt ccaagagagc tgtcaattca gctcccctta atggcgtttc
    11161 caaataatta acccacttga cttccatatc ctgactggac agcaatattc ttaatttttt
    11221 acacttgcta cagttaggat tgtaatagac cgttagcttc tccattaaac caattgtgac
    11281 aagaagcttt cgccaatcat ggctgtgtcc actccgaagt tttccgcaac ggttgctgaa
    11341 atgtccgcaa aatggcctac tggcaaaaca ccgctacctt tgaaagcctt gctgtatgct
    11401 aagagtggca catattcgcg tgtgtgatct gttccagcat aggatggatc attgccgtgg
    11461 tctgcagtaa tcaagagcag gtcatcttca cgcatattgt caataatctc tggaatacgt
    11521 gcatcaaact cttgcaaaca atcgcggtag ccttcgatat tgcgacggtg accatacaca
    11581 gcatcaaagt caactaagtt tgtgaaggag aaaccttcct cgaagtctga taacttcaga
    11641 gtatcaatca acacatcgac accgtgcatg ttggacttgg tgtgacccat atcatttgta
    11701 atacctgaac cattgaaaat atcactgatt ttaccaactg agtaagttgg tacacccgca
    11761 tctgccagtt tgttgagtac tgttgcctta aaaggtgata ctgcatagtc gtgacggttg
    11821 gcagttcgtg agaaattgcc tggctcacca acgtaaggac gggcgatgat acggcctaga
    11881 agagctgggc gttcaagcgt aatggaacga gcgtattcac agatacggta caattcatcc
    11941 agcggaataa tgtcttcgtg ggctgcaatc tgaaggactg ggtctgcgga ggtatagaca
    12001 attaactccc cagtttccat ttggcgaggg ccaaagtcat caatgacagc tgtacctgaa
    12061 tacggcttgt ttgcttcgcg gatgatttta cgacctgaga attcttcgat ttttgtcaaa
    12121 atctcttcag ggaaaccatc ccagaatgta tcaaatggct cagtaatatt gagacccata
    12181 atttcccagt gtccagtcat ggtatcctta ccaagcgata cttcttccaa cttggtcacg
    12241 taaccagttg gatggctttc agcaggaact gtcgcaagcg ctgtatctcg agggatattt
    12301 ccaagaccaa tcttggtcat attaggcacc tcaagcccag ctttttcaga aatgtggccg
    12361 agagtgtcag attctgtatc tgctacaccc gcattgaaaa atttatctga gtctggtgct
    12421 gcgccgattc cgacagagtc cataactacc aagtgaacac gtttaaattt aggcataatt
    12481 gtctccttta tttgatatag ttagattcaa ccaaaatcag cgtccaacca agttgctaga
    12541 ctggaaatta tcattaccac acctaaacat tttaatgggg tgcaatactt atacagtcaa
    12601 agctatcggc atcaaacttc ttaaccatca aaactagata ctgttactcg cttcactgcc
    12661 tgctgttcag ctatcgcttt ttcagtacct attgtctgac tgctacgagg taaggggata
    12721 gatttttgaa gagtctaaac gttcgtgttt tcattatacc ataaaaaagc ctctcattgt
    12781 accaaaatga gaagcgtttt caatttattt cttttcgatg atgttcggtc cttctagtgt
    12841 accaacaatt accttggaaa tcaatccaat aaagagaccg tgttctacaa caccgacggt
    12901 ccgatccagt tcttccgcca atgcatatgt gtcttctatt ctgcgcaaat caaggtcaat
    12961 gataaagttt tgcatatccg tgatatgttt ttctccatta cgcatacgaa aacttggacg
    13021 ataccctttt ttctcaaata gtcggaaaag attttccgca ccgtactgta caacttccac
    13081 cggcaacttg aaggctccta gggtttgaac caccttagac tcatccacaa tccagatgca
    13141 gtccttgcta ttgactgcta ccactttctc catgagaagc gctgcgccac cgcctttaat
    13201 cccattgaag tccccatcaa cctcatctgc tccatccacc gtcaagtcca catattcgac
    13261 ttcgtcgata ttttttaggg gaatcccaag ggacgcagca tgttctgccg tagcatgaga
    13321 ggtcgttacg cctgtgattt gtaacccttc ttctgcaacc cggcggccaa tctcctgcac
    13381 gaaatagtat gccgtcgaac cagtccccag cccaacaatc ataccgtccg tcacaaactc
    13441 cgccgcctta atcccaacct gctcttttag gtttgtcatc tttgtctcct tttaaaaaac
    13501 tgatacaact attatagcat gaagcaagca agacaaaaca agaattttag aaaattgtgg
    13561 gggagatgta ctatcgctta tgattacgat aatataaatc ttgagagtac aaaaactaca
    13621 aatgttatgg ggataactac cagaaggttt tactgttact taaatcttga gagtacaaaa
    13681 accctgcaga tgttaaccca ctcaataatc cgttttactg ttacttaaat cttgagagta
    13741 caaaaactgg ccacgatgcg gtccaaagag ataattagtt ttactgttac ttaaatcttg
    13801 agagtacaaa aacctcaaat ggtttttgta ctctcttccg taattataat atttatcgaa
    13861 aaacttgtca aatttaggaa gaaaggtaat tttcctctaa ttttgata
//
LOCUS       DBNFEG010000037        13795 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.037, whole genome shotgun sequence.
ACCESSION   DBNFEG010000037 DBNFEG010000000
VERSION     DBNFEG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13795
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.037"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(17..1684)
                     /gene="dnaX"
                     /locus_tag="AC1PNG_000771"
     CDS             complement(17..1684)
                     /gene="dnaX"
                     /locus_tag="AC1PNG_000771"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIP6701291.1"
                     /translation="MYQALYRKYRSQTFGEMVGQEVVATTLKQAIEQGKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVGGEPCNDCYICQAITEGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSLATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKSIKVTDIQEHLAAILTKEGLIFDEQALTIIARRAEGGMRDALS
                     ILDQALSLSQDQQVTLEIAEEITGSISLRALDDYVASLRQGDSVTALQHLQTLFDQGK
                     SMSRFATDLLHYLRDILIVQTGGEDTHLTTGFSENLALEQARIFTMIEMVTRGLADIK
                     SSPQPKIYAEMMTIRLAEDSSPSGAMAELPADLLGQLASLQQQVADLQKQLGQLSSQP
                     SAVKPVSRKPQAPKKYRLDTSKVHAILQEAMENPTLARENLTRLQNAWGEIIESLSGA
                     DRALLVGSQPVAANENHAILAFDSPLNAEQTMKRDNLNTMFGNILSKAAGFSPQILAI
                     AQEDWVSIRAEFSAKAKGQKTQEIEKEASPVPEEFGFLADTVEIRND"
     gene            complement(1684..2181)
                     /locus_tag="AC1PNG_000772"
     CDS             complement(1684..2181)
                     /locus_tag="AC1PNG_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIP6701292.1"
                     /translation="MTKQEKISNYQLLLAQLEALLEGETNALANLSNASALLNQALPN
                     SVFTGFYLYDGSELILGPFQGGVSCVHIALGKGVCGEAAAKRQTILVDDVRLHDNYIS
                     CDATALSEIVVPMVKNDQLLGVLDLDSRLVADYDTIDQDYLEKFVALLVEKTDWNFAM
                     FGEKR"
     gene            complement(2246..2515)
                     /locus_tag="AC1PNG_000773"
     CDS             complement(2246..2515)
                     /locus_tag="AC1PNG_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HIP6701293.1"
                     /translation="MSIKQMVSMALVVWNLVVFITYGMDKGKARKNAYRISEKTLLLM
                     SYFGGGLGAWAGGTHFRHKTQKKYFQLAWAIGVLIDAVIMYWMWK"
     gene            complement(2603..3141)
                     /locus_tag="AC1PNG_000774"
                     /pseudo
     CDS             complement(2603..3141)
                     /locus_tag="AC1PNG_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
     gene            complement(3143..3772)
                     /gene="udk"
                     /locus_tag="AC1PNG_000775"
     CDS             complement(3143..3772)
                     /gene="udk"
                     /locus_tag="AC1PNG_000775"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HIP6701294.1"
                     /translation="MTQKPIIIGVTGGSGGGKTSVSRAILDNFPNARISMIEHDSYYK
                     NQSHLTFEERILTNYDHPLAFDTDLMIYHISELLAGRSVDIPIYDYTQHTRSEKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDIRIIRRIKRDMEERGRSLDSIIEQ
                     YTSVVKPMYHQFIEPTKRYADIVIPEGVSNVVAIDLINTKVESILRERG"
     gene            3855..4841
                     /locus_tag="AC1PNG_000776"
     CDS             3855..4841
                     /locus_tag="AC1PNG_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIP6701295.1"
                     /translation="MLNFGIVGTSDISHRFIEAAHLSGHYQLQAVFSRKLETAAAFSE
                     QYEGVDLYTEWVKFLSAPIDVVYIASPNALHFEQAKAVLAAGKHAIVEKPMVSTPQEL
                     EQLRRVAQENNVFLFEAARNYHEQAQAIIREFLTDKTILGGSFGYAKYSSKMPELLAG
                     QMPNIFSTDFSGGALMDLGVYTLYAAIGILGTPRTARYVAQQLPSSIDLNGSGQLIYD
                     NYIVHIQAGKNITSNLASEIYTNDGTLTLNACQYISSAIFTKHDGEQIVLPIQAAQHP
                     MLEEALEIAQAIKQQNRKLVDQWLAVAESVHQTLYTMRQDAGIIFKADNNEI"
     gene            4831..5913
                     /locus_tag="AC1PNG_000777"
     CDS             4831..5913
                     /locus_tag="AC1PNG_000777"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIP6701296.1"
                     /translation="MKSKFPSSWTDQLTLLGFEDFTPIQVQAFEPIANGKSLLAISPT
                     GTGKTLAYLWPSLLALTPKKAQQLLILAPNTELAGQIFDVCKTWSETIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNINTIVLDEFDQLFSDSQYQFV
                     EKIINYVPRDHQLIYMSATAKFDRQKIAEDIESIDLSEQKLDNIQHCYMMVDKRERLE
                     TLRKFANIPDFRALAFFNSLSDLGASEDKLLYNGVNAVSLASDVNVKFRKVIIERFKN
                     HELNLLLATDMVARGIDIDNLECVLNFEVPFDQEAYTHRSGRTGRMGKEGLVITLVSS
                     PSELKQLKKYASVQEVILKNQELYKI"
     gene            complement(5980..7377)
                     /locus_tag="AC1PNG_000778"
     CDS             complement(5980..7377)
                     /locus_tag="AC1PNG_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIP6701297.1"
                     /translation="MKKVSFWLGINIALLGIMVSLAVWLFAGLQERQVSQFIEEKQQT
                     ILAKGKGKIQEGNIDTTHVVAALPTDDAGHVLGPVESRMISYVQRRFGHKKPAGKIQN
                     LVFVSSIEGKTNFKNVTAREIQAEQYKVDNLQIKKQDKLPSERVLLTQDNKLFTLEDL
                     LPNLSSTASIIVGHLREALLAQGMKETDVEAIVKKFETLDLNAISFSYGDSQLTLQLP
                     DGYGINQLVLPISDLYPVVKSDYLVDADKVGYDEYMAAQVVDKKHLRQVALTFDDGPN
                     PNTTPVVLDLLKKYNAKATFFVVGKAVAGNEAILRRMVAEGHVIANHTWNHPNLVTIS
                     GEQVQREIQDTQAAITEATGIVPTMVRPPYGSVNQAVVNQIGLPSIYWSVDSKDWKSR
                     NPQAILKEIKEQTCPGSIILMHDIHQSTVDSLESVLQYLTGEGYNMVTVTDLLASPLN
                     PQLIYYSQELSGPAQ"
     gene            complement(7667..8050)
                     /gene="gloA"
                     /locus_tag="AC1PNG_000779"
                     /gene_synonym="lguL"
     CDS             complement(7667..8050)
                     /gene="gloA"
                     /locus_tag="AC1PNG_000779"
                     /gene_synonym="lguL"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HIP6701298.1"
                     /translation="MASKKMLHACLRVENLEASQKFYEEAFGFKETRRKDYPEHKFTL
                     VYLALEGDGFEIELTYNYDHGPYIVGDGFSHLALSSDDLEGDNAKHKELGYPTTDIKS
                     LPGSPGRYYFVTAPDGYRVEVIRAD"
     gene            complement(8156..8848)
                     /locus_tag="AC1PNG_000780"
     CDS             complement(8156..8848)
                     /locus_tag="AC1PNG_000780"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HIP6701299.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDLAFTSVLKRAIKTTNLALEAADQLWVPVEKSWRLNERHYGGLTGLNKAEAA
                     AEFGDEQVHIWRRSYDTLPPEMAKDHEHSAHTDRRYAHLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKQLSDDEIMDVEIPNFPPLVFELDE
                     NLNIVKEYYLEA"
     gene            9074..10258
                     /locus_tag="AC1PNG_000781"
     CDS             9074..10258
                     /locus_tag="AC1PNG_000781"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIP6701300.1"
                     /translation="MKKVAIVSAYRSAIGSFGGSLKDIEIADLGAQVLETALANKNIP
                     ADSVDEVIFGNVLSAGQGQNIARQIAIRAGIPQTASAYAVNKVCGSGLKSVLLAAQSI
                     MLGDNDVVVAGGIEIMSQAPYLSKTSRFGSKFGHISLEDSMLTDGLTDAFNDYHMGIT
                     AENVAERYHVSRAEQDAFTYSSQEKAAKAIAEGRFVDEIVPIRLKNRKGETIFATDEY
                     PRLTPIEKLATLRPSFKKEGTVTAANASGINDGCAVLVLMSDEKASELNIQPLAYIEA
                     YATSGLDPALMGLGPITASQKALQKLNKTVEDIDLFELNEAFAAQSIPVVKQLGIDPA
                     KVNVNGGAIALGHPIGASGSRILVTLIHELIKQEKELGLCSLCIGGGQGISLIVSNAQ
                     TN"
     gene            10287..11441
                     /locus_tag="AC1PNG_000782"
     CDS             10287..11441
                     /locus_tag="AC1PNG_000782"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HIP6701301.1"
                     /translation="MNIGIDKIGFAAPDYVLDLADLAQARNVDPNKFKIGLLQSEMAV
                     APVTQDIISLGAKAAEAILTEEDKQTIDMVIVGTESSVDQSKAAAVTIHGLLGIQPFA
                     RSIEMKEACYGATAGLSLAKSHIAQFPESKVLVIASDIAKYGVASGGEPTQGAGAVAM
                     LVTANPQILVLNNDNVCQTRDIYDFWRPNYDKYPRVDGKFSTEQYTDCLTTTFDYYQQ
                     KTGKTLNDFAAMCLHIPFSKQGLKGLQAIAQDEETLSRLTERFQEAIVYNKVVGNIYT
                     GSIFLSLLSLLENSRALETGDQILFYSYGSGAVCEIFSGQLVEDYRDHLQENRLEQLN
                     QRTKLSVKEYEQVFFEEITLDETGSSLNLPEDQSPFALIKVDNHKRIYRK"
     gene            11702..12961
                     /locus_tag="AC1PNG_000783"
     CDS             11702..12961
                     /locus_tag="AC1PNG_000783"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HIP6701302.1"
                     /translation="MSTFSGFYKKSRQERIDILRQNRSLSEDSLDILYKDENLPEAIA
                     GKMAENHLGTFSLPFSVLPELLVDGQTYSVPMVTEEPSVVAAASFGAKIIAKSGGFTT
                     TIHNRIMIGQVALYDISDHSRATQAILDHKESILETANQAHPSIVKRGGGARELTVES
                     KDEFLIVYLQVDVQEAMGANILNNMLEAIKDDLEELSKGQALMGILSNYATESLVTAQ
                     CHIAISSLATSSAIAQETAQKIALASKLAQVDPYRAATHNKGIFNGIDAVVIATGNDW
                     RAVEAGAHAYASRDGQYKGLSTWSIDGEHLVGSITLPLPIASVGGSIGLNPKVAVAFD
                     LLQQPKARQLASIIASVGLCQNFAALRALVTSGIQAGHMKLHAKSLALLAGAEEHEVD
                     QLAQLLRKEKHSNLETAQNLLAKMREH"
     gene            complement(12983..13450)
                     /locus_tag="AC1PNG_000784"
     CDS             complement(12983..13450)
                     /locus_tag="AC1PNG_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIP6701303.1"
                     /translation="MTIRKARQSDISVLNELLQDILQVHHQVRPDIFKSAGQKFSEAE
                     LVALLENPDKPIFVFELEGQVVGHLFCELSTTTGDVLEPVKTLFIDDLCVANSARGQK
                     IGEQLYEFALSYAKEQGCHNLTLDVWADNAGAVRFYERQGMKPQKFRMEQIIN"
     gene            complement(13570..>13795)
                     /locus_tag="AC1PNG_000785"
     CDS             complement(13570..>13795)
                     /locus_tag="AC1PNG_000785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701304.1"
                     /translation="FFLFKGQIRGKTLYSMKIKSSLGNEAEDRTGVHQGKLTTDNFDF
                     RRVLVVFYTTHSRFKTTKNQPWPADFLGDL"
BASE COUNT         3991 a         3165 c         2649 g         3990 t
ORIGIN      
        1 ttaggactga tattttctaa tcatttctaa tctcaactgt atctgcgaga aagccaaatt
       61 cttctgggac tggactggct tctttttcga tttcctgtgt cttttgccct tttgctttcg
      121 ccgaaaattc agcacgaata cttacccaat cttcctgagc aatagctaaa atctgtggcg
      181 aaaaacctgc ggccttgctg agaatatttc caaacatggt attgagattg tcacgcttca
      241 tcgtctgttc agcattaagc ggagaatcaa aggctagaat cgcatgattt tcattggcag
      301 caactggttg agaaccaaca agcaaggcac gatctgctcc tgacagactc tcgataattt
      361 ctccccaagc attctgcaag cgagtcaaat tttcacgcgc caaggttgga ttttccatag
      421 cttcctgcaa aattgcatga accttgctgg tatccaatcg gtacttcttc ggagcctgtg
      481 gcttgcgtga aactggctta acagcacttg gttgactaga taattgacct agttgtttct
      541 gtaaatccgc aacttgttgt tgcagactgg ctagttgccc cagtaaatca gctggcagct
      601 ctgccatggc ccccgaagga gaactatctt cagccaaacg aatggtcatc atttcagcgt
      661 aaatcttcgg ttgaggactg gacttgatgt ccgccaaacc cctggtcacc atctctatca
      721 tggtaaaaat ccgtgcctgc tcaagagcta gattttctga aaaacctgtt gttaaatgcg
      781 tatcctctcc acccgtctgc acgataagga tatctctcaa atagtgcaaa agatctgtcg
      841 caaaacggct catactcttt ccttggtcaa acaaggtttg tagatgctgg agagctgtga
      901 cactatcccc ttgacgcaga cttgccacat agtcatccaa ggcacgcaag ctgatagaac
      961 ccgtaatttc ctcagcaatt tccaaggtca cttgctggtc ctgactaagg ctgagagcct
     1021 gatccaaaat ggatagggca tcccgcatcc ccccttctgc ccggcgcgca atgattgtca
     1081 gagcctgctc gtcaaagatt agcccttcct tggtcaaaat tgcagccaag tgctcctgaa
     1141 tgtccgtcac cttgatggac ttaaattcaa atcgttgcac ccttgacaaa atagtagcag
     1201 gaatcttgtg caattccgtc gttgctagaa taaagaccac attttcagtc ggctcttcca
     1261 aggtcttcaa gagggcattg aaggctccag tagacagcat gtggacctcg tcgatgatat
     1321 agaccttata ggtcgcaaga ctaggtgcat aggtcgattt atcacgaata tcacgaatct
     1381 catccacacc attgttggaa gccgcatcaa tctcaatgac atcttccagg ctaccttccg
     1441 taatagcctg acagatataa caatcgttac aaggctcgcc tccaacctga ttgggacagt
     1501 tcatggcctt ggcaaaaatc ttagctgccg atgtcttacc cgtaccacga ggacctgaaa
     1561 agagataggc atgactgatt tttccctgtt caatggcctg tttgagcgtg gtcgcaacca
     1621 cctcctgccc caccatctcc ccaaaggtct ggcttcggta cttcctatat aaagcttggt
     1681 acattagcgt ttttctccaa acatagcaaa attccagtcc gtcttctcaa ccaagagagc
     1741 aacgaacttt tctagatagt cttggtcaat cgtatcataa tcagctacca atctcgaatc
     1801 caagtccaaa acgcccagca actggtcgtt tttcaccatt ggcacaacga tttctgatag
     1861 ggctgtcgca tcgcaggaaa tatagttatc atgtagacga acatcgtcta cgagaatagt
     1921 ctggcgctta gctgcagcct caccgcagac accctttcca agtgcaatat gaacacagga
     1981 aacgccaccc tgaaaaggcc ccaaaatcaa ttcactacca tcatacaagt aaaatcctgt
     2041 aaagacagag tttggtagag cttgattgag caaagcagaa gcatttgaca gattggctaa
     2101 agcattggtt tcgccctcta aaagcgcctc caattgggcc aataatagtt gatagtttga
     2161 aattttttct tgttttgtca taggaacatt atagcatagt taagccaact tttatagtcc
     2221 gagcgataaa gaaaaaggct tacgcctatt tccacatcca atacataatc actgcatcaa
     2281 tcaatacccc tatagcccag gctagttgaa aatacttttt ctgtgtctta tgccgaaaat
     2341 gggttccacc tgcccaggca cccagcccgc caccaaaata ggacatgagt aataaggtct
     2401 tttctgaaat ccgataggcg ttctttcttg ccttcccttt atccatacca taggtaatga
     2461 agacaactaa attccaaact actagcgcca tactaaccat ctgtttgatt gacatacatt
     2521 cctccaatta agtcacattt cttcttactc taacataatc caaaacagga cacaaggaaa
     2581 aacgattcgg aaatccgaat cgttacattt ccgccagtgt atcctctaac catttgagct
     2641 gggcacggtt gcgttcaata gcccgtgtca aaatcaaata gtgtccataa ttgtcttgga
     2701 tactttcctt ggttgagaac agctcaactt tacgactttc taggtgtttt aagtgcttgg
     2761 tgactagttc aatttgactc tgcaacaaac caggaatccg tgggtcatcc ttttcacgga
     2821 taaaaaacat cttgattgaa aacaagtctc tttgctgagg cgtttcttca tttggaatgg
     2881 atagccattc gtctaaaatc tgacgaccag tagccgtcat cgcatactgc ttttcctttt
     2941 catttccagg tactgcatga caagtaatca attcttcctt ggtcatacgt tttaattctg
     3001 gatagacttg gctatggcaa cctgccaaaa ttcgcctaaa tcacgttgaa catagtcggt
     3061 aatctgcttg ccagttacca tttgaccgct agcacccata atccctaata tgatatgagg
     3121 taatattctt tgcttcggca tcttaaccac gctctcttaa tatactctcc accttggtgt
     3181 taatcaagtc gatagctaca acgttgctaa cgccttcagg aatgacgata tctgcatagc
     3241 gcttggttgg ctcgataaac tggtgataca ttggtttcac caccgaggtg tactgctcaa
     3301 tgatactatc taagctacgg ccacgctctt ccatgtctct cttaatgcgg cgaatgatac
     3361 gaatatcgtc atcagtatcg acgaatatct taatatccat taaatcccgc aaccgtttgt
     3421 cttccaatac taaaatacct tccacaataa atacatcttg tggttcctga cgataggtct
     3481 tctctgaacg tgtgtgctga gtgtaatcat aaatcggaat atccactgaa cgtcctgcta
     3541 atagctcact gatatgataa atcatcaaat ccgtatcaaa agccagtgga tggtcataat
     3601 tggttagtat ccgttcttca aaggtcaggt gggactgatt cttataataa gaatcgtgtt
     3661 caatcataga tatacgagca tttgggaaat tatccagaat ggcacgtgac acacttgtct
     3721 tgccaccccc agatccgccg gttacgccaa taataatggg tttctgagtc atcatcatct
     3781 ccttctattt tcaatactat tttaccatga aacatggtat aatgaaaagg ataacacaaa
     3841 agaaatgaga atttatgctt aattttggta ttgttggcac ctcagacatc tctcatcgtt
     3901 ttatagaggc ggcccattta agtggacatt atcagctaca ggctgttttc tcccgtaaac
     3961 tagaaactgc cgctgccttt tcagagcagt atgaaggagt cgatttatac acagaatggg
     4021 taaaatttct ttctgccccc attgatgttg tctatattgc cagtccaaat gccctgcact
     4081 ttgaacaggc aaaagctgtt ctagctgctg gtaaacatgc tattgttgag aagcccatgg
     4141 tttctacacc tcaggaatta gaacaattgc gtcgcgttgc ccaagaaaat aatgttttcc
     4201 tctttgaggc tgctcgaaac tatcacgaac aagctcaagc cattatccgt gaatttttga
     4261 cggataaaac gattttaggt ggttcatttg ggtatgccaa atattcttct aagatgcctg
     4321 aattgttagc tgggcaaatg ccaaatatct tttcaactga tttttccggt ggtgctctca
     4381 tggatttagg tgtctatacg ctctacgccg ctatcggcat tttgggaact cccagaacgg
     4441 cccgctatgt agcacaacag ctaccttcta gtattgactt aaatggttcg ggacaactca
     4501 tttatgacaa ctatatcgtc cacattcaag caggaaaaaa tatcacgagc aatctagcga
     4561 gcgaaatcta tacaaatgat ggaacactaa cactcaacgc atgccagtat atcagctctg
     4621 ctattttcac aaaacatgac ggagagcaaa ttgttttacc aatccaagcg gcccagcatc
     4681 ctatgctcga agaagcgcta gaaattgcac aggcaatcaa gcaacaaaac cgcaagcttg
     4741 tggaccagtg gttggctgtg gcagaaagcg tccatcaaac actttacacc atgcgtcaag
     4801 acgcaggaat aatctttaag gcggataaca atgaaatcta aatttccctc tagctggacg
     4861 gaccagctta ccctacttgg ctttgaggac tttactccta tccaagtcca ggccttcgag
     4921 cctattgcaa acggcaagtc ccttctagct atcagcccaa caggcacagg aaaaaccctt
     4981 gcctacctct ggcctagcct attagcccta actcctaaaa aagcccaaca attgctcatc
     5041 ctcgcaccca atacagagct ggccggccaa atatttgatg tttgtaagac ttggtcggag
     5101 acaattgggt tgactgctca actattcttg tctggttcca gtcaaaaacg ccagattgag
     5161 cgactaaaaa aagggccaga aatcctgatt ggaacacctg gtcgtatttt cgagttgatt
     5221 aaattgaaaa aaatcaagat gatgaatatc aacaccatcg tcctagatga gtttgatcag
     5281 ctattctccg attctcaata tcagtttgtt gaaaagatta tcaactatgt acctcgtgac
     5341 caccagttaa tctacatgag tgcaacagct aaatttgacc gtcaaaaaat tgctgaagac
     5401 attgaaagca ttgatttatc tgagcagaaa ttggataata tccagcattg ctatatgatg
     5461 gtagataagc gtgaacgttt ggaaactcta cgtaagtttg ccaatatccc tgattttcgt
     5521 gctcttgcat tttttaacag cctatcagac cttggtgcaa gcgaagataa actactctat
     5581 aatggtgtaa atgcagtttc attggcttct gatgtcaacg taaaattccg caaggtcatc
     5641 atcgaacgtt tcaagaatca cgaactcaat cttctacttg ctacagatat ggtcgcacgc
     5701 ggtatcgata ttgataactt agaatgtgtc ctcaactttg aagttccttt tgaccaagaa
     5761 gcctacactc atcgttctgg acgtactgga cgcatgggca aggaaggtct tgtcatcacc
     5821 ctagtatcta gtcctagcga attaaaacaa ttgaaaaagt atgcctccgt ccaagaagtc
     5881 attctcaaga atcaagagct ctacaaaata taatgaacaa tatacaaaag gcttagaatc
     5941 atgttagtag aaacactggt tctaagcctt ttatcattct tactgggctg ggccagataa
     6001 ttcttgtgaa taatagatta attgaggatt gagaggactg gcgagtaagt ctgttacagt
     6061 aaccatattg tagccctcac ctgttaaata ttgcaataca gactcgaggc tatcaactgt
     6121 tgactgatga atatcatgca ttaggataat acttcctgga caagtctgtt cttttatttc
     6181 tttcaaaatg gcttggggat tgcgactctt ccaatcttta gaatcgaccg accaataaat
     6241 cgatggaagt cctatctgat tgacaacagc ctggttaacc gaaccataag gagggcgaac
     6301 cattgttggt acaatacctg tagcttcagt aatagctgcc tgtgtatctt gaatttctct
     6361 ctgtacttgc tccccagaaa tagtgactag attgggatga ttccacgtat ggttggcgat
     6421 gacgtgccct tctgctacca ttctacggag aatagcttca ttcccagcta cagccttacc
     6481 aacaacaaag aaagttgctt tggcattata ttttttcagt aaatctaaaa ctaccggtgt
     6541 tgtgttcgga tttggtccat catcaaaggt tagagcgacc tgtctcaaat gtttcttatc
     6601 cacaacttgt gcagccatat actcatcata gccaacctta tcagcatcta ctagataatc
     6661 actcttcact actgggtaca aatctgaaat tggtagaacc aactggttga tgccgtaacc
     6721 atctggtaat tgtaaggtta attggctgtc tccataacta aaggaaatag catttaagtc
     6781 tagtgtttca aattttttga caatcgcttc tacatcagtc tctttcattc cttgagcaag
     6841 tagggcttct cttagatgac caacaataat acttgctgta gaggataaat taggtaataa
     6901 atcttccagg gtaaacaatt tattgtcttg cgtcaacaac acacgctcag acgggagttt
     6961 atcctgcttt ttaatttgca agttgtctac tttatactgc tccgcttgaa tttcacgagc
     7021 tgttacattt ttaaaatttg tttttccctc tattgatgaa acaaagacca ggttttgaat
     7081 ttttcctgct ggttttttat gaccaaagcg tctttgaaca taggaaatca ttcgactttc
     7141 tacaggtcct aagacatgac ctgcatcatc tgtaggtaag gcagccacaa catgggtcgt
     7201 atcaatattt ccctcttgaa ttttgccctt gcctttagcg aggatggttt gttgtttttc
     7261 ctcgataaac tgactaacct gtctttcttg aagtcctgca aataaccata cagccaaact
     7321 taccattatg ccgagcaagg cgatgttgat tcccaaccaa aaactaactt ttttcatatc
     7381 cagcctccca ttcaaaacat atataaacta tgctaaatca tttaaaggct cttgtcaaga
     7441 cagttctata aaaactcata aaaagattag gtaatactct tcgaaaatca aaattatccg
     7501 ttgtcaactt gccttgatga actccagttc tatcttcggc ttcgttttct aggctacttt
     7561 tgattttcat taagtataaa taattgattt gcttcctaat ccctcccttc tgccattttc
     7621 tgattttttt gaaaagaaaa agactgccta ggcaatcttt tctaaattaa tctgcacgaa
     7681 tgacttccac acggtagcca tctggagctg tcacaaaata ataacgacct ggacttcctg
     7741 gcaagctctt gatgtctgtc gttggatagc ccagctcctt atgcttagca ttgtctcctt
     7801 ctaaatcatc tgaagaaaga gctagatgcg agaaaccgtc gcctacgata tagggaccat
     7861 gatcataatt gtaggtcaat tcaatctcaa agccatctcc ttcaagtgct aagtaaacca
     7921 aggtaaactt gtgctctgga tagtccttac gacgggtttc cttaaatcca aatgcttctt
     7981 cataaaattt ttgagatgct tctaagttct caacacgcaa acaagcgtgc aacatttttt
     8041 tacttgccat aataaattcc tcctaccatt taataccacc agtataccat actctctatg
     8101 aaaaatccga tattcaaaac taaaaaagcc tgagcgaact caggccttaa atgaattatg
     8161 cttccaagta gtactctttc acaatgttca agttttcatc caattcgaat acaagtggtg
     8221 ggaagtttgg aatttccaca tccatgattt cgtcatcaga caattgtttg atgtgtttta
     8281 caagggcacg gattgagtta ccgtgtgcac ccacgaatac gtttttaccg tctttcaatg
     8341 ctggagcaat tttatcttcc cagaatggaa gggcacgctc aagcgttact ttcaagtttt
     8401 ctgcatctgg aataactgaa tcatcaaggt gtgcatagcg acggtcagtg tgtgctgaat
     8461 gctcatgatc tttagccatt tctggtggca aagtatcata agaacgacgc cagatgtgaa
     8521 cttgttcatc accaaattca gcagctgctt cagctttgtt caagccagtc aaaccaccgt
     8581 agtgacgttc gttcaaacgc catgattttt caactggtac ccacaattga tctgcagctt
     8641 caagagccaa gtttgtagtc ttgatagcac gtttcaatac agatgtgaaa gcaaggtcaa
     8701 attcgatacc tgcttctttg atcaatttac ctgcatcgat tgcttgttgt gtaccttttt
     8761 cagacaaatc aacatcagcc caaccagtga aaaggttagc tttgttccat tcagactcac
     8821 catggcgagc aaaaaccaat tttaccattt atggattctc ctttaaattt tgaggttccc
     8881 ctcttatact tctctatttt acacgaaaag gtaaaaaaat gccagccttt ccacaatctg
     8941 tgtacgcttt cattactctt tcatgtaaat tcagtctcta gacctatctt tcttcttaga
     9001 aaaaatacaa aataatggta taatagtaag gctgaacacg acagttcaaa acgataccac
     9061 tataggagtt atcatgaaaa aagtagctat cgtgtctgcc tatcgttctg ccatcggcag
     9121 tttcggaggt agtctgaaag atattgaaat cgctgattta ggggcgcaag tcctcgaaac
     9181 agccttagct aataaaaaca tccctgcaga cagcgttgat gaagttatct tcggaaatgt
     9241 tctttcagca ggacaaggac aaaatattgc acgtcaaatt gctattcgtg caggcattcc
     9301 tcagacagcc tctgcctatg cagtcaataa agtctgtggt tccggtctca aatcagtcct
     9361 cctagctgcc cagtcaatca tgctaggtga caatgatgta gttgtggctg gaggcattga
     9421 aattatgagc caggcacctt atctatccaa aactagccgg tttggtagta aatttggtca
     9481 tattagtctg gaagattcca tgttaacaga tggtttgaca gatgccttca acgactatca
     9541 catgggcatt actgcagaaa atgttgctga acgctatcat gtcagtcgcg cagagcaaga
     9601 tgcttttaca tacagtagcc aagagaaggc tgcaaaagct attgctgagg gaagatttgt
     9661 tgacgaaatt gttcctatta gattaaaaaa tcggaaaggc gaaactattt ttgccacaga
     9721 tgagtaccct cgtctgacgc caattgaaaa attggcaaca cttcggccat cttttaaaaa
     9781 ggaaggaacc gtcaccgcag ccaatgcttc tggcatcaac gacggctgtg ctgtcttggt
     9841 actcatgtct gatgaaaaag ccagtgagct aaatatccag ccactcgcct atattgaggc
     9901 ctatgcaaca agcggtttag atcctgcatt gatggggctt ggacctatta ctgctagcca
     9961 aaaagcgcta caaaaactga ataaaacagt cgaggatatc gatctattcg aactcaatga
    10021 agcattcgca gcccaatcaa ttccagttgt caaacaactc ggcattgatc ctgcaaaagt
    10081 caatgtcaat ggtggtgcta tagcccttgg acatccaatc ggagctagtg gtagtcgaat
    10141 cctagtcact ctgattcacg aattaatcaa gcaagaaaaa gagcttggtc tttgctcact
    10201 ttgtatcgga ggcggtcaag gcatctccct aatcgtttcc aatgcgcaaa caaattaacc
    10261 tatcatttgt acacaaagga tatattatga acatcggtat tgataaaatc ggatttgcgg
    10321 cacccgatta tgttttagat ttggctgatt tagcccaagc ccgcaatgtg gacccaaata
    10381 aatttaagat tggtcttctt cagtctgaaa tggctgtagc tcctgtcact caagatatta
    10441 tcagtcttgg ggcaaaggct gccgaagcca ttctgacaga agaagataaa caaaccattg
    10501 acatggttat tgtcggcaca gaatctagcg tagatcaaag taaggctgcc gccgtcacca
    10561 ttcatggatt actcggtatt cagcccttcg ctcgctccat cgagatgaag gaggcttgtt
    10621 acggtgcaac ggctggacta agtcttgcca aaagccatat cgctcaattc ccagaatcaa
    10681 aagtcctagt cattgctagt gatattgcaa agtatggagt cgcttctggc ggtgaaccta
    10741 cccaaggtgc tggggcagtt gccatgttgg taaccgcaaa cccacaaatt ttggtcctca
    10801 acaatgataa tgtctgccag acacgtgaca tctacgactt ctggcgtcct aactacgaca
    10861 aatatccacg tgttgatggt aaattttcaa ctgaacagta caccgattgc ttgacgacaa
    10921 ctttcgacta ctatcaacaa aaaacaggca aaaccctcaa cgattttgct gccatgtgct
    10981 tacatattcc tttctccaaa caaggtctta aaggattgca agctattgct caggatgaag
    11041 aaactcttag tcgcttgaca gaacgtttcc aggaagctat cgtctataat aaagtcgtcg
    11101 gcaacatcta cactggctct atcttcctct ctcttctttc ccttttggaa aatagcagag
    11161 cgttagagac aggggatcaa atcctctttt atagctatgg tagcggcgct gtctgtgaaa
    11221 ttttcagcgg tcaactcgtt gaagattatc gcgaccactt acaagaaaat cgtttagaac
    11281 aactcaatca gcgtacaaaa ctcagcgtta aggaatatga acaagtcttc tttgaagaaa
    11341 ttacccttga tgaaaccggt tcatccctta acctaccaga agaccaaagt ccctttgccc
    11401 ttatcaaagt ggacaatcac aaacgtatct atcgtaaata agcattttac ctatcattca
    11461 ttggaataat gaatgatagg ctgatcgaat ttccagcaca cttttcacag ttagcgtcaa
    11521 catctcagcg cagtggttga ttggcagatt tattcgtgtt accacactct aaatctgacc
    11581 taatcaactg tgcgggggcg ggaagacgaa ctcttttcta tcagtcaagt tctttcccgc
    11641 tccccaaaac ggcgcccttt ggctctcacc aaggggcgct ttcctataga aagaggaacc
    11701 tatgtcaact ttttccggat tttataaaaa atcacgccaa gagcgcattg atattctccg
    11761 tcagaatcgt tccctatccg aagatagctt agacattcta tacaaagacg aaaacctgcc
    11821 agaagcaatt gcgggaaaga tggcagaaaa tcatctagga acgttttcct tacctttttc
    11881 tgtacttcca gaacttctag tggatggaca aacttactct gtaccgatgg ttactgaaga
    11941 accttctgtg gttgcagctg catctttcgg agccaaaatc atcgctaaat ctggcggttt
    12001 tacaacgact attcacaacc gtatcatgat tggacaagtt gctctctacg atatttcgga
    12061 ccatagcaga gcgacacagg cgattcttga ccataaggag agtattttag aaactgccaa
    12121 tcaagcacac ccttccattg tcaaacgagg gggcggtgca agagaattga ctgttgagag
    12181 taaggatgag tttttgattg tctatcttca ggttgatgta caagaagcca tgggagccaa
    12241 tatcctcaat aacatgttag aagctattaa ggatgactta gaagagctca gcaagggaca
    12301 ggctttgatg ggaatcctct ccaactatgc aacggagtca ctggttaccg cccaatgcca
    12361 tattgccatc tctagtctgg ctaccagctc tgccattgct caagaaactg ctcaaaaaat
    12421 agccctggca agcaaactag cacaagtgga cccttatcgt gctgcgacac ataacaaggg
    12481 gatttttaat ggtatagatg ctgtcgttat cgctacagga aatgactggc gggcagtcga
    12541 agccggggcc catgcttatg ctagccgtga tgggcaatac aaggggcttt ctacttggtc
    12601 cattgatggc gagcacttgg tcggctccat caccctacct cttccaattg cctccgtcgg
    12661 tggctctatc gggctcaatc caaaggtagc agttgctttt gaccttctcc aacaaccaaa
    12721 ggcacgccaa ttagcttcta tcatcgcttc tgtcggtctc tgtcagaact ttgctgccct
    12781 gcgtgctctt gttacttcag ggattcaggc tggtcacatg aagctccacg caaaatccct
    12841 agcgctactt gctggcgccg aggagcatga agtagatcaa ctagcccaac tcctgcggaa
    12901 agaaaagcac agcaacctag aaactgctca aaacttatta gcaaaaatga gagaacatta
    12961 aaaaagaggg actccctctt ttttaattta ttatttgctc catacggaac ttttgtggtt
    13021 tcattccttg gcgttcataa aaacggacag ctcctgcatt gtcagcccat acatccaagg
    13081 taagattgtg gcaaccctgc tccttagcat aggaaagagc aaattcatac agttgctcac
    13141 caattttttg accacgcgcc gaattggcta cacacaaatc atcaataaac aaggttttaa
    13201 ctggttctaa aacatcgcct gttgtggtag ataattcaca aaaaagatgc ccaaccactt
    13261 gtccctctaa ctcaaagaca aaaataggct tatctggatt ttccaaaagt gctactaatt
    13321 cagcctcaga aaatttttga cctgcacttt taaaaatatc tggtcgtacc tggtgatgca
    13381 cctgtaaaat atcttgaagc aattcattta ataccgaaat atcagactgt ctagctttac
    13441 ggattgtcat aatactctcc ttttgagttt ttattattat agcacatgtt cattttttaa
    13501 caaaataggt ctacagaccg atgaagaatt tgaaaattcg agtatactat aggtgtatcc
    13561 taaaattttt cataaatctc ctaaaaaatc agctggccaa ggctggtttt ttgttgtctt
    13621 aaaacgagaa tgtgtagtgt agaaaactac taatactctt cgaaaatcaa aattatccgt
    13681 tgtcaacttg ccttgatgaa ctccagttct atcttcggct tcgtttccta ggctactttt
    13741 gattttcatt gagtataaag ttttgcccct tatttgcccc ttaaacaaga aaaaa
//
LOCUS       DBNFEG010000038        13601 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.038, whole genome shotgun sequence.
ACCESSION   DBNFEG010000038 DBNFEG010000000
VERSION     DBNFEG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13601
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.038"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(1..229)
                     /locus_tag="AC1PNG_000786"
                     /pseudo
     CDS             complement(1..229)
                     /locus_tag="AC1PNG_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(433..1775)
                     /gene="tnpC"
                     /locus_tag="AC1PNG_000787"
                     /pseudo
     CDS             complement(433..1775)
                     /gene="tnpC"
                     /locus_tag="AC1PNG_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(1729..1935)
                     /locus_tag="AC1PNG_000788"
     CDS             complement(1729..1935)
                     /locus_tag="AC1PNG_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HIP6701305.1"
                     /translation="MDELLAIIKQQTSTIEQLTNELTLLREQVDYLRQKLYGKSSEKV
                     VYQPGQLSLFGEESLLEEEADLPS"
     gene            complement(2052..2339)
                     /gene="tnpB"
                     /locus_tag="AC1PNG_000789"
     CDS             complement(2052..2339)
                     /gene="tnpB"
                     /locus_tag="AC1PNG_000789"
                     /inference="COORDINATES: protein motif:HMM:NF017528.6"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HIP6701306.1"
                     /translation="MTIRLSDLGQVFLVCGKTDMRQGIDSLAYLVKSQFNLDPFSGQV
                     YLFCGGRKDRFKALYWDGQGFWLLYKRFENGKLTWPNNEEEVKSLTSEQVD"
     gene            complement(2320..2505)
                     /locus_tag="AC1PNG_000790"
     CDS             complement(2320..2505)
                     /locus_tag="AC1PNG_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701307.1"
                     /translation="MSQPVVQLTIPQSRRFEKKSRNDILMKIRLGKTEVTFFQSINPE
                     VMETILDKVLHYDHPTQ"
     gene            complement(2741..3614)
                     /locus_tag="AC1PNG_000791"
                     /pseudo
     CDS             complement(2741..3614)
                     /locus_tag="AC1PNG_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681261.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(3811..5043)
                     /locus_tag="AC1PNG_000792"
     CDS             complement(3811..5043)
                     /locus_tag="AC1PNG_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylyltransferase domain-containing protein"
                     /protein_id="HIP6701308.1"
                     /translation="MEPICLIPARSGSKGLPNKNMLFLDGVPMIFHTIRAAIESGCFK
                     KENIYVSTDSEVYKEICETTGVQVLMRPADLATDFTTSFQLNEHFLQDFSDDQVFVLL
                     QVTSPLRSGKHVKEAMELYGKGQADHVVSFTKVDKSPTLFSTLDENGFAKDIAGLGGS
                     YRRQDEKTLYYPNGAIYISSKQAYLADKTYFSEKTAAYVMTKEDSIDVDDHFDFTGVI
                     GRIYFDYQRREQQNKPFYKRELKRLCEQRVHDNLVIGDSRLLALSLDGFDNISIGGMT
                     ASTALENQGLFLATPIKKVLLSLGVNDLITDHPLHMIEDTIRQLMESLVSKAEQVFVT
                     TIAYTLFRDSVSNEEIVQLNDVIVQSASELGISVIDLNEVVEKEAMLDYQYTNDGLHF
                     NQIGQERVNQLILTSLTR"
     gene            complement(5052..5702)
                     /locus_tag="AC1PNG_000793"
     CDS             complement(5052..5702)
                     /locus_tag="AC1PNG_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HIP6701309.1"
                     /translation="MIDRGESLMKKVAFLGAGTFSDGVLPWLDRSRYELIGYFEDKPI
                     SDYRGYPVFGPLQDVLTYLDDGKVDAVFVTIGDNVKRKEIFDLLAKDHYDALFNIISE
                     QANIFSPDSIKGRGVFIGFSSFVGADSYVYDNCIINTGAIVEHHTTVEAHCNITPGVT
                     INGLCRIGEGTYIGSGSTVIQCIEIAPYTTLGAGTVVLKSLTESGTYVGVPARKIK"
     gene            complement(5692..6825)
                     /gene="neuC"
                     /locus_tag="AC1PNG_000794"
     CDS             complement(5692..6825)
                     /gene="neuC"
                     /locus_tag="AC1PNG_000794"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HIP6701310.1"
                     /translation="MKKICFVTGSRAEYGIMRRLLSYLQDDPEMELDLVVTAMHLEEK
                     YGMTVKDIEADKRRIVKRIPLHLTDTSKQTIVKSLATLTEQLTVLFEEVQYDLVLILG
                     DRYEMLPVANAALLYNIPICHIHGGEKTMGNFDESIRHAITKMSHLHLTSTDEFRNRV
                     IQLGENPNHVLNIGAMGVENVLKQDFLTREELAMELGIDFAEDYYVVLFHPVTLEDNT
                     AEEQTQALLDALKEDGSQCLIIGSNSDTHADKIMELMHEFVKQDSDSYIFTSLPTRYY
                     HSLVKHSQGLIGNSSSGLIEVPSLQVPTLNIGNRQFGRLSGPSVVHVGTSKEAIVGGL
                     GQLRDVIDFTNPFEQPDSALQGYRAIKEFLSVQASTMKEFYDR"
     gene            complement(6837..7853)
                     /gene="neuB"
                     /locus_tag="AC1PNG_000795"
     CDS             complement(6837..7853)
                     /gene="neuB"
                     /locus_tag="AC1PNG_000795"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HIP6701311.1"
                     /translation="MVYIIAEIGCNHNGDVHLARKMVEVAVDCGVDAVKFQTFKADLL
                     ISKYAPKAEYQKITTGESDSQLEMTRRLELSFEEYLDLRDYCLEKGVDVFSTPFDEES
                     LDFLISTDMPVYKIPSGEITNLPYLEKIGRQAKKVILSTGMAVMDEIHQAVKILQENG
                     TTDISILHCTTEYPTPYPALNLNVLHTLKKEFPNLTIGYSDHSVGSEVPIAAAAMGAE
                     LIEKHFTLDNEMEGPDHKASATPDILAALVKGVRIVEQSLGKFEKEPEEVEVRNKIVA
                     RKSIVAKKAIAKGEVFTEENITVKRPGNGISPMEWYKVLGQVSEQDFEEDQNICHSAF
                     ENQM"
     gene            complement(8375..9781)
                     /locus_tag="AC1PNG_000796"
     CDS             complement(8375..9781)
                     /locus_tag="AC1PNG_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIP6701312.1"
                     /translation="MSKKSIVVSGLVYTIGTILVQGLAFITLPIYTRVISQEVYGQFS
                     LYNSWVGLVGLFIGLQLGGAFGPGWVHFREKFDDFVSTLMVSSIAFFLPIFGLSFLLS
                     QPLSLLFGLPDWVVPLIFLQSFMSVVQGFFTTYLVQRQQSMWTLLLSVLSAVINTALS
                     LFLIFSMENDFIARVMANSATTGVFACVSLLFFYKKIGLHFRKDYLRYGLSISIPLIF
                     HGLGHNVLNQFDRIMLGKMLTLSDVALYSFGYTLASILQIVFSSLNTVWCPWYFEKKR
                     GADKDLLSYVSYYLAIGLFVTFGFLTIYPELAMLLGGSEYRFSMGFIPMIIVGVFFVF
                     LYSFPANIQFYSGNTKFLPIGTFIAGVLNISVNFVLIPTIGIYGAALATTASYLLLLV
                     LHYFVAKKKYAYDEVAISTFVKVIALVVVYTGLMTVFVGSIWIRWSLGIAVLVVYAYI
                     FRKELTVALNTFREKRSK"
     gene            complement(9790..10761)
                     /locus_tag="AC1PNG_000797"
     CDS             complement(9790..10761)
                     /locus_tag="AC1PNG_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 52"
                     /protein_id="HIP6701313.1"
                     /translation="MRRIYICHTMYQILISLLKMDVERDSLMSVDIIGHFPDVREQLQ
                     QHVHLIEETERSFDLYSLIARSKTKERLSLLQSYDEVIIFQDHRQVGHFLNKHRIPYS
                     LLEDGYNFFKDKRVCDLESIQSSVWKRLFYQWYFKPTYLIGSSLYCQSIEVNDLSLVQ
                     FDEAYKPFVEVPRKQLFDQASPEKVQALLQIFGARAIVADEESSQKRLLLLTQPLSWD
                     YHVTEEELLEIYVAGLAPYREDYTIYIKPHPRDGVDYSFLGKAVVLLPQGIPFELFEM
                     AGSIRFDIGMTYSSSALDFLNCFEEKVYLKDTFPLLSKNDILREGIE"
     gene            complement(11198..11311)
                     /locus_tag="AC1PNG_000798"
                     /pseudo
     CDS             complement(11198..11311)
                     /locus_tag="AC1PNG_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936306.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylyltransferase domain-containing protein"
     gene            complement(11545..11784)
                     /locus_tag="AC1PNG_000799"
     CDS             complement(11545..11784)
                     /locus_tag="AC1PNG_000799"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701314.1"
                     /translation="MIKYGQKIPATLLSKLGVASYHIYLTQMLYFSVVAPFLAVQFKV
                     SSLNLWNGLFTFLICLFGGYIFYKVDLFMRVRGKR"
     gene            complement(12253..12564)
                     /locus_tag="AC1PNG_000800"
     CDS             complement(12253..12564)
                     /locus_tag="AC1PNG_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701315.1"
                     /translation="MNPTNSRIALFDTIKCIMVLCVIFTHLDWSVEQRQWFIFPYFVD
                     MAVPIFLLLSAYFRTNKWNTKQETLKLKFSSGIKESINMLCLYAIVMAVNVLLSYSRT
                     I"
     gene            complement(12604..13599)
                     /locus_tag="AC1PNG_000801"
     CDS             complement(12604..13599)
                     /locus_tag="AC1PNG_000801"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIP6701316.1"
                     /translation="MSIIVPIYNVEQYLSKCINSIVNQTYKHIEILLVNDGSTDNSEE
                     ICLAYAKKDSRIRYFKKENGGLSDARNYGISRAKGDYLAFIDSDDFIHSEFIQRLHEA
                     IERENALVAVAGYDRVDASGHFLTAEPLPTNQAVLSGRNVCKKLLEADGHRFVVACNK
                     LYKKELFEDFRFEKGKIHEDEYFTYRLLYELEKVAIVKECLYYYVDRENSITTSSMTD
                     HRFHCLLEFQNERMDFYESRGDKELLLECYRSFLAFAVLFLGKYNHWLSKQQKKLLQT
                     LFRIVYKQLKQNKRLALLMNAYYLVGCLHLNFSVFLKTGKDKIQERLRRSESSTR"
BASE COUNT         4191 a         3004 c         2391 g         4015 t
ORIGIN      
        1 tggttgaaat acccactttc attttcttct taaccatcat ttcaggagaa aatttttgct
       61 tagtatagtg taaaattttc tctttcaatt ctttcgttag gattatcttt tttaccgatc
      121 gttttcgatt agtttggtat accgtttgtg catagtcagc agaatagact tttttgtaca
      181 gcccttttcg gacttgttgt aaagttgtcc cacgtttgac ttcattatag aactttcata
      241 agtagcttag acttgctgtt tttgattttt attgatactc ttcgaaaatc aaaattatcc
      301 gttgtcaact tgcctttatg aactccaact ccagttctat cttcggcttc gtttcctagg
      361 ctactttgta accgcccaat aataaggtat attgaaaagg ctccaaagtc ctgttgactc
      421 tggaaccttt ttctatttac atgctctctt aattttcttc tcccatggca aataagcctc
      481 tagaacttct tttttagcga gtgtttcctc gttaggtaga ttgtctagaa gataggtcat
      541 atatttctct gtatcaagtt cgtgcctctt ggccgtttcc aaaagactca aaatgatagc
      601 tgtcgacttg gctccctcaa aactttggga gaacaaccaa ttttttctgc ccatcaccaa
      661 ggtcttcata gccctttcag ccacgttgtt agacaggacc aagtcaccat ccgagagaac
      721 ggttcggaag gtggtttcgt atttgaggct atactccaaa gcagtaccca atttggagcc
      781 tggcaaaaca gattgctcac gacaccagtc aaagaactcg tccatcaagg gagccagcgt
      841 ttatgcagtc gctcctcgct agacaagtca gcccagtcgc tctccaaggc aaacaggcgg
      901 tcgcaatagg ctaagccctt ggctcctaaa gaagtcttgt ctgtcttctt aggagtcgcc
      961 tcaaagaact ttcgtttgac gggagcccaa caaccaacca gctgagcctt gtctaattga
     1021 cgataagcac tccacatgtc acaatgtacg tagcccgcat agtccccaag aacctcctcc
     1081 acaaccaagc cactccgtcg tttgtcatga tgatagaggg taattccatt ttcctcatgt
     1141 ttcccagaca agaaggtcca gtagaaggtc aactgaccat cattttctaa gaccttgtag
     1201 gcggtctcat ccgcatggag aataggctgc tccaacaatt tctcgtgcaa caggttgtaa
     1261 atcggctcga aataatactg actagacttg atatgccagt tggctatttc cttccgactg
     1321 atgggcaggc caagcttatg ccaatcctct tcctgacggt aatttggcac cttcaaattg
     1381 aacttctgat gaatggtgtg agcgatgata gaggctgaac ccaagctgtg tgccaaaggt
     1441 gccttaggaa cgggagcctt gataatctta tcgctcagat tcttctggct acatgcctga
     1501 tacttgtatg cgtgttgaac atggtcaatc cgctttaatt gtgcaggaat gaagaccaac
     1561 tcttgccgtt gaacagctga gccaatctct ttcaactgac catgacagtc tggacaagtg
     1621 cagtcttcgc cctgcaattc gtgatgcact atctctggag tgaactgact aaaaatagcc
     1681 tgacgaactc ccttagtttt cttgcgttta taagtgatga tctctgtttc aactgggtaa
     1741 gtcagcttct tcttcaagga ggctttcctc cccaaacaag ctcagctgac cgggttgata
     1801 cacaactttc tctgatgatt ttccgtagag tttctgtctc agatagtcaa cctgttctcg
     1861 aaggagggtt agctcattgg taagttgttc aatcgttgaa gtctgttgtt taataattgc
     1921 caacaactca tccatacgct cgctcctcct tctttttatc tcattataca caaagaaaag
     1981 ccatgatttc aatagaaatc acgacttttt gaaacattta tttttggaat gatagaaaat
     2041 cctttcatga gctaatctac ttgttcggaa gtgagggact taacctcctc ttcattgttt
     2101 ggccaggtca atttcccatt ttcaaaacgt ttatacaata accaaaatcc ctgtccatcc
     2161 cagtaaagag ctttgaatcg gtcttttcga cctccgcaga agagatagac ctgacctgaa
     2221 aagggatcca gattaaattg acttttgaca agataggcga gagagtcaat cccctgacgc
     2281 atatccgttt tcccacagac caagaagact tgacctaaat cactgagtcg gatggtcata
     2341 atgcagcacc ttatccaaga ttgtttccat tacttcaggg ttgatagatt ggaagaatgt
     2401 gacttccgtt tttccgagac gaattttcat cagaatatcg tttctgcttt tcttttcaaa
     2461 acggcgagat tggggaatag tcaattggac gacgggctgt gacataatga atcctccagt
     2521 ttgtttttta taacagtata atggaggaat gtgtgattgg atagatacct tgttattagg
     2581 cggttacgct ttaaacatca aaaatgagag aacgaatttc gtcctctcta ggtgttagct
     2641 tttcgtctac ccactacagt tgacaaagag ccgttttttt attgtgagaa actagttttt
     2701 gcccagcctc tttttgctta ttttcctatt tttgggcgca ctaagcccac gatggtaatc
     2761 ccagtcgttt gatttgctca aaacgacaga tattgtagag caggttggtt aaggtcacat
     2821 tggttttagc tcgtgccttc ccaattgctc gacagatgtt acctttcatg ttagtttcaa
     2881 tgaagccaaa accatgctca acgcgacaac ggactttggt aatatgtcga ttaatgacct
     2941 tatcagtctc agtcaacggt ttatttctaa aagcacgacg aatcgtgtgg tggcgacaac
     3001 cttctggtac tgattttcca acataagcac tgtcatcaaa aaccgcttca ttggcatcac
     3061 aaagaggagc taggacattg gagtcgtgta cactagctgt tgtgacctca taattttcga
     3121 taagttttga ttttctgatt gtcctcacgt gaattgcgtt gtttagggca ttcgacaaag
     3181 gtagcatcca caatctgacc tgaatgggca atcacccctt catctgtgag atgggcatag
     3241 aacaaatcga acaactcctt ttcacgacct gacttggtta atttctcacg atagagccag
     3301 atagttttcg catcgggaac agtgtcttca tgacaaccaa caaaacgacg aaaagatata
     3361 cgatccagca gttgatattc catggcatcg tcagataggt tatgaagacg ttgaagcaag
     3421 agcgctttga acatcatgag atagtctagg tgaggacgac cgccacgact gatgacttta
     3481 tctttacgac tgaataactc tgacaataat ggaagaaaga tattccaatc cataacggca
     3541 tccaaacgtt ctaagggatt gcctttctct gatagttttg acaagatttt ttcatcgtct
     3601 gtgaataaat gcatgacagc tctcctcatt actttttatt ctattatctc atttttggga
     3661 aatttaggga gtttttagag gtccccttat ataattaaac aactaaatgg ttgtcaattc
     3721 tcaaggctag catcctctac agatgttatt ccttggctct aaaacacata ccaacatagt
     3781 ctagccactg aaatagcttc tatcaccaaa ttatcttgtc aaacttgtca aaatcagctg
     3841 attcacacgc tcttgtccaa tctgattgaa atgcaatcca tcattggtat actgatagtc
     3901 aagcatcgcc tctttttcaa caacttcatt tagatcaatc actgaaatac ccagttcact
     3961 tgctgactga acaataacgt cattcagctg cacaatttct tcatttgaaa cgctatcacg
     4021 aaacagcgtg taggcaatcg tcgtcacaaa aacctgctct gctttggaaa caagactttc
     4081 catcagctgg cgaatagtat cctcaatcat atgcaaggga tggtcagtaa tcaaatcatt
     4141 cacaccaaga gaaagcaaaa ctttctttat cggagtagcc aaaaagagac cttggttttc
     4201 aagtgctgtc gaagctgtca tcccaccgat gctgatatta tcgaaaccat ccagtgacaa
     4261 ggctaacaga cgactatcgc caatcacaag attatcatgg actcgttgct cacataaacg
     4321 ctttaactct cttttataaa atggtttgtt ttgttgctca cgacgctggt aatcaaagta
     4381 aattcgacca ataacaccag taaaatcaaa gtgatcatct acatcaatcg aatcttcctt
     4441 cgtcatcaca taggccgctg ttttttcaga aaaataagtt ttatccgcta aataagcctg
     4501 cttagaagaa atataaatcg ctccattagg atagtagagt gttttctcat cttgacgacg
     4561 ataactgcca cctaatcctg caatatcctt agcgaatccg ttttcgtcta aagttgaaaa
     4621 caatgttgga gacttatcga ctttggtaaa gctaacaacg tggtcagctt gacctttccc
     4681 atataactcc atcgcctcct tgacatgttt tcccgatctt aatggggacg taacttgcag
     4741 gagaacaaat acttggtcat cagaaaaatc ttgtaaaaaa tgttcgttca gttgaaaaga
     4801 ggttgtaaaa tctgtcgcca agtcagctgg acgcatgagg acttgaaccc cagttgtttc
     4861 acaaatttcc ttgtaaacct ctgaatcagt actgacatat atattttctt tcttaaaaca
     4921 tccagactca atcgcagctc gaatggtatg gaaaatcatc ggtacaccat ctaaaaataa
     4981 catgttttta tttggtaaac cttttgatcc tgaccgagca ggaatcagac aaattggttc
     5041 catcaattca cctatttaat ctttctagca ggtacaccaa cataggtccc tgactccgtc
     5101 aacgatttca aaacaactgt ccctgccccc aatgttgtat aaggtgcaat ctcgatacat
     5161 tgaatcactg ttgaaccact tccaatataa gtgccttctc cgatacggca caagccattt
     5221 atggtcactc ctggagtaat gttacaatgg gcctccaccg tggtatgatg ttccacaatg
     5281 gcacccgtat tgatgataca attgtcatag acataggaat cggctcctac aaaacttgaa
     5341 aaacctatga aaacccctcg tcccttgata ctatctgggg aaaaaatatt ggcttgctcg
     5401 ctaatgatgt tgaacaaagc atcataatga tctttggcaa gcaagtcaaa gatttccttg
     5461 cgcttgacat tgtcacctat agtgacgaag acagcatcta cttttccatc atccaaatag
     5521 gttaggacat cttgcaaggg accaaataca ggatagccac gatagtcact gatcggttta
     5581 tcttcaaaat atccaatgag ttcatatcga cttctatcca accaaggaag gacaccatct
     5641 gaaaaggtac ccgctcctag aaaggctact tttttcatca aactttctcc cctatctatc
     5701 ataaaactct ttcatggttg aggcctgtac agataaaaat tccttgatag ctcgataacc
     5761 ttgtaaagca gaatcaggtt gttcaaatgg attggtaaaa tctatcacat cacgtaattg
     5821 ccccaaacca ccaacaatcg cttccttaga agttccaaca tgtaccacac tcggtcctga
     5881 caaacgtcca aattggcgat ttccaatatt taaggttgga acctgtaatg agggcacttc
     5941 aatcaaacct gacgaagaat tccctattaa accttgtgaa tgcttgacca aggaatggta
     6001 ataacgagtt ggaagcgaag taaagatgta agaatcagag tcttgtttta caaattcatg
     6061 catcaattcc attatcttat cggcatgtgt atccgaattg gatccaatta tcaaacactg
     6121 gctaccatct tcttttagag catctaataa ggcctgcgtt tgttcttcgg ctgtgttatc
     6181 ctccaaggta acagggtgaa agagtacaac atagtaatcc tcggcaaaat caattccaag
     6241 ttccatcgcc aactcttctc ttgtcaaaaa gtcttgtttt aaaacatttt caacacccat
     6301 agctccgatg ttcagtacat ggttgggatt ttctcctagt tgaatgacac gatttctaaa
     6361 ttcatccgtt gatgtcagat gaaggtgact catcttggta atggcatggc gaatcgactc
     6421 atcaaaattt cccatggttt tttcaccacc atgaatatgg caaataggaa tattataaag
     6481 caacgcagca ttggcaactg gtagcatctc atagcgatcc cccagaatca acaccaagtc
     6541 atactggact tcttcaaaaa gaaccgtgag ttgctctgtc aaggtcgcta aagatttgac
     6601 gattgtctgc ttagacgtat ccgtcaaatg caatggaatc cgcttgacaa tcctacgctt
     6661 gtccgcttcg atgtctttga ccgtcatccc atatttttct tctagatgca tggctgtcac
     6721 tacaagatcc agctccattt ctggatcatc ctgtagatag ctcaataagc gacgcataat
     6781 cccatattcg gcacgagagc ctgtcacaaa acaaattttt ttcatcctta ctccgcttac
     6841 atttgatttt caaaagcact atggcaaata ttttggtctt cctcaaaatc ctgctcactc
     6901 acctgcccca agactttgta ccattccatt ggcgaaattc catttcctgg tcttttgaca
     6961 gtgatgtttt cttctgtaaa gacttcgcct ttagcaattg cttttttggc aacaatagat
     7021 tttctagcta caattttatt tcgtacttca acttcttctg gctctttttc aaatttacca
     7081 agagattgtt ccactatcct cactcctttt accaaggctg ctaagatatc aggagtagca
     7141 ctcgctttat gatctggtcc ttccatttca ttgtccagag taaagtgctt ttcaatcaat
     7201 tcagctccca ttgctgcagc agcgatgggt acttctgaac caacactatg gtctgaatag
     7261 ccaattgtta agtttggaaa ttcttttttc aaggtatgca agacattcaa attcaaagca
     7321 gggtaagggg ttggatactc ggttgtacaa tgcaaaatcg aaatatcggt cgttccattt
     7381 tcctgcaaaa tcttcaccgc ttgatgaatt tcatccataa cagccatacc agttgaaaga
     7441 ataactttct tagcttgacg accaattttt tccaaatagg gaagattggt aatctcacca
     7501 gatggaatct tataaacggg catatctgtg ctaatcaaga agtccaatga ttcctcatca
     7561 aaaggtgtcg aaaacacatc aactcccttt tcaagacagt aatcacgcaa atcaagatac
     7621 tcttcaaagc tcaattccaa acgacgagtc atttcgagct gagaatctga ctctcctgtt
     7681 gtaatttttt ggtattctgc ctttggtgcg tattttgaaa tcaacaaatc tgccttaaat
     7741 gtctgaaatt taacggcatc cacaccacaa tcaacggcaa cttctaccat tttccgtgct
     7801 agatgaacat caccgttgtg attacaacca atttctgcaa taatataaac cattttattt
     7861 cctttctaaa aatgactgta aatcgctccc ataaagtctt gagggagcag cttggttttc
     7921 cttaagtagt aaaagaacac taataaaaat cgctacttca cttctgatag ccgattagaa
     7981 aaaatagccc tagatttctt tcgaatagtc tcagggcttg ttgcttctaa agcctgcttc
     8041 ttcacacgat cttcaataga ttggtggaag tttaatctgc ttttctaccc aatttttagg
     8101 gagatttacc tcatgacttc tggcataagc agaggcgcat acgtccttta agaaacattc
     8161 ccttctttat acttgattat agcacaattt tcatggtaga gaatacttga actttgggta
     8221 gatggcagtg atggtcaatt cttatgagca cctctcattt aggacgtttt tatatgggga
     8281 cctctaaaaa ctaattttct aaaatttaaa atgttgattt aacaaggctt tcgtaactcc
     8341 aaattcgcaa ttttgggagt ttatagaggt gcccttattt agaccgtttt tccctgaatg
     8401 tattgagggc aactgttaat tcctttctaa aaatgtaggc ataaacgact agaaccgcta
     8461 ttcctagtga ccaacgaatc cagattgaac cgacaaatac tgtcatcaag cctgtataga
     8521 cgacaacaag agcaattacc ttaacaaatg ttgaaatcgc aacttcatcg taagcatact
     8581 ttttcttagc aacaaaataa tgcaagacta gcaacaacag ataggaagca gtcgttgcca
     8641 aagcagcacc ataaattcct atcgtcggta tcaaaacaaa gttgactgaa atatttagta
     8701 cacctgctat aaaagtacca attggcaaaa actttgtatt tccactataa aactggatat
     8761 tggctggaaa actataaaga aatacaaaga acaccccgac aataatcatg ggaataaatc
     8821 ccatactgaa acgatactca gatccaccta acaacatcgc taattcaggg taaattgtta
     8881 gaaatccaaa agtcacaaac aggccaatcg ccagatagta actgacataa ctgagcaaat
     8941 ctttatctgc acctctcttt ttctcaaaat accacggaca ccataccgta ttcaagctcg
     9001 aaaacacaat ttgtaagata gacgcaagtg tgtagccgaa actgtatagg gctacatctg
     9061 acagtgttag catcttgccg agcatgattc tgtcaaattg attgagtaca ttatgaccta
     9121 atccatgaaa aataagagga atcgatatac ttaaaccata ccgaagatag tcctttcgaa
     9181 aatgaagccc aatcttctta tagaaaaaca acaaggacac acaagcaaaa acaccagtcg
     9241 ttgccgagtt tgccattaca cgagcgatga aatcattctc catcgaaaag atgagaaata
     9301 aagataaagc agtgttaata acagcgctca gtaccgatag gagtaaagtc cacatggact
     9361 gctgccgctg cactaaatag gtcgtaaaaa atccttgcac aacactcata aaactttgca
     9421 aaaagataag cggaacgacc caatcaggca aaccaaatag gagcgatagg ggctgactga
     9481 ggagaaaaga tagcccaaaa attggtaaaa agaaagcgat agaagagacc atcaaggtgg
     9541 atacgaaatc atcaaatttc tcgcggaagt gtacccatcc cgggccaaaa gccccaccta
     9601 actgtagacc gataaagaga ccaactagcc ccacccacga attatacaag ctaaactgcc
     9661 catatacttc ctgagaaatg acacgagtat agatggggag ggtaatgaag gctaatccct
     9721 gaacgaggat ggttccaatc gtatagacga gacctgagac aactattgat tttttagaca
     9781 tgaatcctcc tattctatcc cctcacgcaa aatatcattt tttgaaagaa gaggaaaagt
     9841 gtcctttaaa tacactttct cttcaaaaca atttaaaaaa tctaaagcag acgaactata
     9901 ggtcataccg atatcaaaac ggatactacc tgccatttcg aacaactcaa acggaatacc
     9961 ttgaggcaga agcaccacag ccttacccag aaatgaataa tcaaccccat ctcgtgggtg
    10021 cggttttatg tagattgtat agtcttctcg ataaggggca agacctgcta cataaatctc
    10081 caacaactcc tcttcggtca catgataatc ccaagacaag ggctgggtca ataatagcaa
    10141 tcgtttttga gaagactcct catccgctac tatcgccctt gctccaaata tctgcagcag
    10201 cgcttgcacc ttctctggcg atgcttgatc aaataattgc tttctcggaa cttctacaaa
    10261 gggtttataa gcctcgtcaa attgtacgag cgacagatca ttgacctcaa tggattgaca
    10321 atagagactt gaaccaatca aatatgttgg tttaaaatac cattgataaa agagtctttt
    10381 ccagacagat gattgaattg actccaaatc gcacactctt ttatccttga aaaaattata
    10441 accatcctcc aaaagagaat agggaatccg atgtttattt aaaaaatgac cgacttgacg
    10501 gtgatcttga aaaatgatca cctcgtcata gctctgtaac aaggaaaggc gttcttttgt
    10561 ttttgatcta gctatcaaag aatatagatc aaatgaacgc tccgtctcct cgattagatg
    10621 aacatgctgc tgcagttgct ccctgacatc tggaaaatgc ccgatgatat caacggacat
    10681 caaactatct ctctcaacgt ccatctttaa caaggaaatc aggatctgat acatcgtatg
    10741 gcaaatataa attcgtctca atttttctcc ttctacatac agactttaag gataagtaac
    10801 ccctttttat aacccctgta cagcttcgat aatgtactga ttcactcttt cttgcccttc
    10861 atcattgaaa tgcaaaacat tttctgagtc cccctgtaag tacatattaa tggcttcttt
    10921 tatctgttcg ccagtccgta acggtgaggt gacttgcaga agaacaaata tacaatcttc
    10981 ataaaatcca gattcgtaaa aaagtgcgta ctgacttcat tcgatggagt atgataagta
    11041 gatagttctt tgggtcttaa caatacattg atgcctcttg tctcgcaaat ccccttgggg
    11101 acctctaaaa actaattttc taaaatttaa gatgttgatt taacaaggct ttcgtaactc
    11161 caaattcgca attttgggaa tttatagagg tgcccccctt atacatttct gaatccgtac
    11221 tgacatagat gtcttctttc tcaaaacaac ctgattcaat tgccacatca atcgtgtgaa
    11281 aaatcatggg tttcccgtcc aaaaatagca tgtttttatt cgataagcct ttcgaaccag
    11341 atcgtgctgg gattaaacaa acagttctct taaaaattct ccttgttcag ttaataaata
    11401 tagctttgaa aataaagggg gatactgggg tttagaaaca gataccactc ctctctcgtc
    11461 aacctatttg aatttctttg tcggaatatt aacatgcgaa tctgctaata aacgaaatgg
    11521 catctgctaa tctgaaatga gtcattatcg ttttccacgt actctcataa acagatccac
    11581 tttgtagaaa atatagccac caaacaggca aattagaaag gtaaacaagc cgttccacaa
    11641 attcaacgaa gataccttaa attgcactgc taaaaatggt gcgactactg aaaaatacag
    11701 catctgggtc aagtagatat gataagaagc aactcccaat tttgacaaca gtgttgctgg
    11761 aatcttctgt ccatacttta tcataaaaaa tagcatagca tacgcaaatg ggacgcatag
    11821 aaaggaagta gacttccaac cataaaacca ggagaagggc tctatagaat gattcacaaa
    11881 aatcagaatt gctccaataa tcccaaatag ggtcgcaatg aaagtatcta ccttggaacg
    11941 cacatcctgc tttgaaagaa aaaaccaagc cctagaacaa aaagataacg aagtgatatt
    12001 agtctatata tatatgccgt gttcagccat gttagcaaat atggcatcca ctgaaaagtt
    12061 tactaaaaaa caagtaagca agcccaacca tttatttttc tcgaaaagaa catacaaaat
    12121 tggtaataaa aaaactacct gaatcaacaa cggaacatag taactcccaa ctccctggac
    12181 ccgattctcc agaaagtagc cacaggacaa atgaacggag cgatgaagaa cctgaaaaag
    12241 gctttactcc tatcagatgg ttctcgaata gctcaataaa acattaacag ccatcacgat
    12301 agcatagaga caaagcatgt ttatactttc ttttatacca ctgctgaact tgagctttag
    12361 cgtctcttgt tttgtattcc acttattcgt tcgaaaatag gcagaaagca acagaaaaat
    12421 tggaacagcc atgtcaacga aatacggaaa gataaaccat tgacgctgct caacagacca
    12481 atccagatgt gtaaaaataa cacaaagtac catgatacat ttaatcgtat caaagagtgc
    12541 tattctacta tttgttggat tcattttaat ccccttttct ttcaaccatt tattacaaca
    12601 ttcttaccga gtactacttt cacttcttct caatctttct tgaattttat ctttccccgt
    12661 tttcagaaag acactaaaat taagatgaag acaccctacc aaataataag cattcattag
    12721 taaagcaagt cgcttatttt gcttcaattg tttatataca attctaaata gcgtttggag
    12781 aagcttcttt tgctgtttgc tcaaccaatg attatatttg cctaaaaaca aaacagcaaa
    12841 ggctaaaaat gaacgataac actctagtaa gagctcttta tctcctctac tttcatagaa
    12901 gtccattcgt tcattttgaa attccagtag gcaatggaag cgatggtcag tcatgctaga
    12961 agttgtgata ctattttctc ggtcaacata atagtacaag cactccttaa ctattgcaac
    13021 tttttctaac tcatagagca agcgataagt gaagtattca tcttcatgaa tcttaccctt
    13081 ttcaaatcga aaatcttcaa atagttcttt tttatagagt ttattacagg ccaccacaaa
    13141 gcgatgacca tccgcctcta gcagcttttt acaaacattc ctgccgctca gaacagcctg
    13201 atttgtagga agcggctctg ctgttaagaa atgccccgaa gcatctaccc tatcataacc
    13261 agcaactgcc acaagggcat tctctctctc aattgcttcg tgtaaacgtt ggatgaactc
    13321 cgaatgaata aaatcatctg agtctataaa agctaagtag tcacccttgg cgcgacttat
    13381 gccataatta cgggcatctg atagcccgcc gttctctttt ttaaaataac gaatgcgact
    13441 atctttcttc gcatatgcta aacaaatttc ttccgaatta tccgtactac cgtcattcac
    13501 cagaagaatc tctatatgtt tgtaggtctg atttacaatg ctatttatac acttggatag
    13561 atattgttca acattgtaaa ttgggacgat gatagaaatg t
//
LOCUS       DBNFEG010000039        13492 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.039, whole genome shotgun sequence.
ACCESSION   DBNFEG010000039 DBNFEG010000000
VERSION     DBNFEG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13492
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.039"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            248..388
                     /locus_tag="AC1PNG_000802"
     CDS             248..388
                     /locus_tag="AC1PNG_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701317.1"
                     /translation="MKKKEQSSRQIVICHLVTILGVDIVKTTQLIDEFGNVGILVLEG
                     QS"
     gene            385..1188
                     /locus_tag="AC1PNG_000803"
     CDS             385..1188
                     /locus_tag="AC1PNG_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HIP6701318.1"
                     /translation="MKRITANQYQTSERYYKLPKVLFESERYKDMKLEVKVAYAVLKD
                     RLELSLSKGWIDEDGAIYLIYSNPNLMALLGCSKSKLLSIKKTLREYGLIDEVQQSSS
                     ERGRMANKIYLGELEHEPTPVLHTDGASVKKTLGGSQRKTGPVLYSAPSETEGSETKG
                     SDFFIEDEEERQLVDEKQEENFTSKVDGVTKYDRDYIWGLVHDQLRQTGLSQSASDYA
                     MIYFSDRYQYALEHMRFARSAEVIAEYVFNGVLSEWTKQLRRQEVKGGE"
     gene            1337..2692
                     /gene="dcm"
                     /locus_tag="AC1PNG_000804"
     CDS             1337..2692
                     /gene="dcm"
                     /locus_tag="AC1PNG_000804"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HIP6701319.1"
                     /translation="MKFLDLFAGIGGFRLGMESQGHKCVGFCEIDKFARTSYKAMFNT
                     EGEIEYHDIKEVTDHDFRQFRGQVDIICGGFPCQAFSLAGRRLGFEDTRGTLFFEIAR
                     AAKQIQPRFLFLENVKGLLNHDEGRTFATILSTMDELGYDVEWQVLNSKDFQVPQNRE
                     RVFIIGHSRRYRSRFIFPLRRENSPAHLERLGNINPSKRGLNGEVYLTSGLAPTLTRG
                     NGEGAKIAIPVLTPDRLEKRQHGRRFKDNQDPMFTLTSQDRHGVVVAGNLPTSFDQTG
                     RVFDISGLSPTLTTMQGGDKVPKILLREELPFLKIKEATKTGYAKATLGDSVNLAYPD
                     STKRRGRVGKGISNTLTTSDNMGVVVAALEYRQDKWYEVTGIVLEGKLYRLRIRRLTP
                     RECFRLQGFPDWAYERAESVSSKSQLYKQAGNSVTVTVIEAIAREFRRTEEEEKHEPT
                     T"
     gene            2676..3104
                     /locus_tag="AC1PNG_000805"
     CDS             2676..3104
                     /locus_tag="AC1PNG_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701320.1"
                     /translation="MNLLHKKSILDCTELEEHIHQVETKQLLQKILSLPNFDCDFEVT
                     FEDDYHKEMNDPLFYESNLHRISDFLETRDIKNGVDTLLTKDNHLAFRAFGENYSARG
                     KEGILTTLVTVKCFGEGRMPIDMSRYFSTPEPTVENSLTL"
     gene            3115..3498
                     /locus_tag="AC1PNG_000806"
     CDS             3115..3498
                     /locus_tag="AC1PNG_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701321.1"
                     /translation="MLEVYLGNNANTNQDLLTILTTYGVTYRCIKACDVNREILLSLF
                     AKTMDCFELLSPRFLRFKRQYSISLNEMIQLILQKPDQNLRLPLIVCQNHVYPAIGLD
                     EVRTFLPRQVKEELFQASLMKQVTG"
     gene            3507..3740
                     /locus_tag="AC1PNG_000807"
     CDS             3507..3740
                     /locus_tag="AC1PNG_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIP6701322.1"
                     /translation="MNERFWDNLEIILAEKDLTWAELARKVFNGQYVYPSEFNRLYQK
                     LRHYKSNRLMPQTRWVERIVLVLEIDYEDLFKR"
     gene            3743..4327
                     /locus_tag="AC1PNG_000808"
     CDS             3743..4327
                     /locus_tag="AC1PNG_000808"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIP6701323.1"
                     /translation="MIRIVVFYLAIQLNGFLLSLYLQEYMTIEGIVLLQLVLLSVTCL
                     EIARHKTVQAKNITLRNRLRWFLLGFVAMVAFAVFISFLFPGQTRNQAVLVQVGKQVP
                     PIIFLLFLVNASLLEEIVYRQLLWEKLTFPFEQIGVTSFLFVLSHGPNQLGSWLMYSC
                     LGLTLAVVRLKTDCMTAIALHLLWNSLAYVVTFL"
     gene            4405..4893
                     /locus_tag="AC1PNG_000809"
     CDS             4405..4893
                     /locus_tag="AC1PNG_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701324.1"
                     /translation="MSSEQQERQAMQYVERSSLLTVRTLLKLLEWSARQALAQDSAYK
                     IGVQKLEELLQSPYAIEAIDVSKDILDKPIDVEKFKELMESEKLPIAISWQKDYLYFY
                     AKDKTLLDHHLDELLKKLMSNPEKLEGLIFDKTLDQEIELAKEKIRVTEPSAVKTKEV
                     TL"
     gene            4893..6710
                     /locus_tag="AC1PNG_000810"
     CDS             4893..6710
                     /locus_tag="AC1PNG_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HIP6701325.1"
                     /translation="MYSRQKAFVFGLLGLAFGYFCHRLTLLYDSLTNAPPMERFAYLL
                     GEGGNQVFNPLWLFSFTQKSLLAFILGVLTMTLVYLYVSTGQKVYREGEEYGSARFGN
                     SKEKRNFYSKNPFNDTILARDVRLTLLEKKKNQFDRNKNLIVIGGSGAGKTFRFVKPN
                     LIQLNCSNIVVDPKDHLAEKTGKLFLENGYQVKVLDLVNMTNSDGFNPFRYVETENDL
                     NRMLTVYFNNTRGSGSRSDPFWDEASMTLVRAIASYLVDFYNPPGSSKQEQEARRKRG
                     RYPAFSEIGKLIKLLSKGDNQDKSVLEVLFEDYAKKYGHENFTMRNWADFQNYKDKTL
                     DSVIAVTTAKFALFNIQSVIDLTQRDTMDLKTWGTQKTMVYLVIPDNDTTFRFLSALF
                     FSTVFSTLTRQADVDFKGQLPIHVRSYLDEFANVGEIPDFAEQTSTVRSRNMSLVPIL
                     QNIAQLQGLYKEKEAWKTILGNCDSLLYLGGNDEETFKFMSGLLGKQTIDVRSTSRSF
                     GQTGSSSTSHQKIARDLMTADEVGNMKRDECLVRIAGVPVFRSKKYFPLKHKNWKWLA
                     DKESDERWWHYRINTLAAEEELDLSGHTIRDLSTETSLH"
     gene            6728..6970
                     /locus_tag="AC1PNG_000811"
     CDS             6728..6970
                     /locus_tag="AC1PNG_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701326.1"
                     /translation="MNQKLTGFVYGVDASSMFSQAMSLLQKGLIAVGAFLVVMGIINL
                     STNIKDGGPGVRNAILEIVGGVMVGAAGAFVTQITI"
     gene            6989..7843
                     /locus_tag="AC1PNG_000812"
     CDS             6989..7843
                     /locus_tag="AC1PNG_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL"
                     /protein_id="HIP6701327.1"
                     /translation="MMMNFTSPFVFLASEKVSSDSLFEGFQVDLESTANLVKSLADFN
                     PTVWSYMTAITKGIMQPLGVAILAVVLVLEFSKMAKKIANSGGAMTFEAMAPMIVSYI
                     MVAVVITNTTVIVEAIIALASYVIEQVAGLVTNGGANYDTISGIKGSGIVGKMIIGFF
                     AILIWLVRMASIMVVNILITIRFIQLYLMIPFAPVTIPTFLSDDWRSVGIGYLKNIMV
                     YAVQGILIFLIVSLVPLFESAGKIAVSNGAGVMESLAIMFGGLVQAILLIIALVGSQR
                     TARSILGM"
     gene            7905..8258
                     /locus_tag="AC1PNG_000813"
     CDS             7905..8258
                     /locus_tag="AC1PNG_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HIP6701328.1"
                     /translation="MNTRVFKDISKVQHRAWLGFTTRQVIFVFPAIALTILVLGLNLF
                     YWQFGDWFVYGFVFSFTIPLMLFGVYRPNDLPFETYLKYRFHYELTVPDRTFTGRKGD
                     QREKIKTLNETKDLF"
     gene            8215..10566
                     /locus_tag="AC1PNG_000814"
     CDS             8215..10566
                     /locus_tag="AC1PNG_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HIP6701329.1"
                     /translation="MKKLKHSMKPKTSSNDKKQKTKAQKQEIKPSTVNTLAYQGLFQN
                     GLMQVSPSYFSQTYLLGDVNYQTVGLDDKGAIVEKYSDLINSLDDKTNFQLTIFNQKV
                     NLEKFRKSILYPLQEDGFDAYRDELNRMMDANLEAGENNFSAVKFLSFGKSDQTPKLA
                     FRSLSQIGEYFKSGFSEIDVSLGLLGGEERVNVLADMLRGENHLPFSYKDLTLSGQST
                     KHFMAPTYLSFKHKNHIELDDRLLQIVYVRDYGMELGDKFIRDLMQSDLEVMISLHAK
                     GSTKSETMTKLRTKKTLMESQKIGEQQKMARTGIYLEKVGHVLENNIDEAEALLQTMT
                     QTGDKLFDTVFLIGVLADTEDQLKQSLDIIKQVAGSNDMIIDNLTYMQEAAFNSLLPF
                     GKNYLEGVSRSLLTSNIAVNAPWTSVDIQDKGGKFYGINQISSNIISIDRGKLNTPSG
                     LILGTSGAGKGMATKHEIISTKLKEADSDTEIIIVDPENEYSIIGQAFGGESIDIAPD
                     STTFLNVLDLSDENMDEDPVKVKSEFLLSWIGKLLDRKMDGREKSLIDRVTRLTYKHF
                     VTPSLVEWVFVLAQQPEQEAKDLALDMELYVEGSLDIFSHRTNIKTDSHFLIYNVKKL
                     GDELKQIALMVTFDQIWNRVVKNQKLGKKTWIYFDEMQLLLLDKYASDFFFKLWSRVR
                     KYGAIPTGITQNVETLLLDANGRRIIANSEFMILLKQAKSDREELVHMLGLSKELEKY
                     LVNPEKGAGLIKAGSTVVPFKNKIPQHTKLFDIMSTDPEKMRT"
     gene            10568..13369
                     /locus_tag="AC1PNG_000815"
     CDS             10568..13369
                     /locus_tag="AC1PNG_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HIP6701330.1"
                     /translation="MKEDKKLVKQARQNFRSNLKSARMHYRKEVRALKQTVPKKGRFR
                     KSAQNSLFQEKKTELKENLLSSQKEAEEKFLKEITYVSPRLLKVKEIKNYRLPQAQER
                     LRTARKHLSEVKLNEKQQAVNPKFTFQKENPSLKSRFQFHQEKSFDRLSAEKDVSSAK
                     REVKQLKKVRKSKKSSTKVKAGLGLAASESLDLIAQDDDLDGLRTLTDTSLKVRRYGR
                     FTFQAGKVVVKSGQTSVRFTKTKFSHGKERFQNFKKGKGFTRQKPLKPRRRYQTFLKL
                     ARKQSVAGFKGIVQAIKGSMTFFSVLAGNPLTWIVSGILLILLLMMSFFMSVSGSSVI
                     QQDEMELTKAYTHITWEDAEHTRTNEKGITFYTKIDEVMVYMNHQYQDYKLDDFMEAG
                     GVTYKEFLSQLWSDLNGRDSIKSMSDLYKEPAYKLSDEDQEELKELTEEGNYLALQEL
                     DNPFQGQTDEDSLNMTYRYGYEVIDEKPTLHHHIILEAKEGQVIVSPMDGKVSLDGEN
                     VILISGKGVNKTKLTLFGIHSGRVSENQQVLAGDIIGQTKDGMGLKVTYQKVDDDTDK
                     LVYVNPAFYFPKVIQVQTTILPTIGQFGGDEFERAKAIYDYLKSKGATDQAIAAILGN
                     WSVESSINPKRAEGDYLSPPVGATDGSWDDEGWLSLNGPTIYNGRYPNILKRGLGLGQ
                     WTDTSDGSRRHTLLLEYAKGKHQKWYDLGLQLDFMLHGDSPYYTNWLKDFFKNSGSPA
                     SLAQLFLIYWEGNSGDKLLERQTRASEWYYQVEKGFSQPNGGTAQSDPKALEAVRGDL
                     FENSIPGGGDGMGYAYGQCTWGVAARINQLGLKLKGKNGEKIPIISTMGNGQDWVRTA
                     ASLGGETGTSPQAGAILSFAGGGHGTPTKYGHVAFVEKVYPDGSFLISETNYNGNPNY
                     TFRKLSGVDSSLSFAYTTK"
BASE COUNT         4152 a         2478 c         3039 g         3823 t
ORIGIN      
        1 ggagtgcgta gtgttgctac aacgaagcaa cgggtaaaaa tcctttattt taagcacttt
       61 ttcaaacatt ttgtctttat tgaaaagagt gattttgaca taaaaaaggt tcaaaaaaag
      121 tacattgatg tgaacgtctg tttggatgtc gactgcgtag acaaaaaata gatacgtcat
      181 aaaatataat agttcagcga gaacatttta ataagtgttc tcgttttttt attttcagga
      241 ggaattcatg aaaaagaagg aacaatcatc acgccaaatc gtgatatgtc atctcgtgac
      301 tattttagga gttgatattg tgaaaacaac tcagctaatt gatgaatttg gaaatgttgg
      361 aatattagtc ttggagggac aatcgtgaaa cgtattaccg ctaatcaata tcagacatca
      421 gagcgttatt ataaactccc aaaagtcttg tttgagagtg aacgttataa agatatgaag
      481 ctggaagtta aggtagccta cgcggtttta aaggatcggt tggagttgtc tttgagtaaa
      541 ggttggattg atgaggatgg ggctatatat ttgatttatt ccaatccaaa tctgatggca
      601 cttttaggct gttcaaagtc aaaactactc tctatcaaga aaaccttacg cgaatatggc
      661 ttaatcgatg aagtccaaca gtcctctagt gaaagaggac gaatggccaa taagatttac
      721 ttgggggagt tggaacatga acctacccca gtcttacata cagacggggc tagtgttaaa
      781 aaaacactag gggggtctca aagaaagacg gggccggtct tatattcagc ccctagtgag
      841 actgaaggaa gtgaaactaa agggagtgat ttctttattg aggacgagga ggagaggcag
      901 ttagtagatg agaaacaaga agaaaacttt acttcaaaag tcgatggcgt gaccaagtac
      961 gatcgagact atatttgggg tttggttcat gaccagttaa gacagacagg tctatctcag
     1021 tcggctagtg actatgccat gatttatttt agtgaccgtt atcagtatgc tttggaacat
     1081 atgcgatttg ctcggtcagc ggaagtaata gctgaatatg tatttaatgg tgtgctatcg
     1141 gagtggacca agcaactgag acgacaagaa gtaaagggag gtgaataagg tgatttggtg
     1201 gatactaggt ggaatctatc tgatttctat cgtcattttg atttttgaaa tcatccgtgc
     1261 accagaaatg gatgatcata tataggcaag agttttacaa gtgtaaggct cttttttagt
     1321 tggaaagtga ggaaaaatga aatttttaga tttatttgct gggataggtg gttttaggct
     1381 aggaatggaa tcacagggtc ataaatgcgt gggcttttgt gaaattgata aattcgccag
     1441 aacatcttat aaagccatgt ttaacacaga aggggaaata gaataccatg acattaaaga
     1501 ggtcacagac catgacttta gacaatttag agggcaagtg gacatcatct gcgggggatt
     1561 tccttgccaa gcattttcac tcgcaggcag acgattggga tttgaagata ctcgagggac
     1621 tctctttttt gagattgctc gagcggccaa acaaatccaa ccacgttttc tatttttgga
     1681 aaacgtcaaa ggcctactca atcacgacga gggacggacg ttcgccacta tcctctccac
     1741 gatggatgaa ttggggtatg atgtcgaatg gcaggtgctt aacagtaagg acttccaagt
     1801 cccgcaaaac agagagcggg tctttattat cggacattct agaagatacc gttccagatt
     1861 catatttcct ctcagaagag aaaacagccc agctcatctt gaaaggctag gaaatatcaa
     1921 tccctctaaa cgtggtttga atggtgaagt ctatctgacg agtggacttg ctcctacact
     1981 aacaagaggt aatggagagg gagctaaaat cgccattcca gtcttaacac cagatagact
     2041 agaaaaacgg caacatggtc gtcgatttaa ggacaatcaa gaccctatgt ttactttgac
     2101 cagtcaagac agacatgggg ttgttgtcgc aggaaatctg ccgactagct ttgaccagac
     2161 tggaagagta ttcgatatat ctggcttgtc accgactttg accaccatgc aaggtggaga
     2221 caaggtgcca aaaattttgc tgagggagga gctgccattt ctgaaaatca aggaagccac
     2281 aaaaacaggg tacgcaaagg caactcttgg agactctgtc aatctggctt atccagactc
     2341 aaccaaacgt aggggacgtg tgggaaaggg aatatccaat actctgacga cttcagacaa
     2401 tatgggagtg gtggttgctg ctctggaata tcgacaggat aagtggtatg aagtcacagg
     2461 cattgtctta gaggggaaac tttatcgcct gagaataaga cgactgactc caagagagtg
     2521 tttcagactt caaggctttc cagactgggc ttatgaaaga gcagagagtg tttctagtaa
     2581 gagccagcta tacaaacagg ccggcaatag cgtgactgtc acagttattg aagctattgc
     2641 cagagaattt agaagaacgg aagaggaaga aaaacatgaa cctactacat aagaaaagta
     2701 ttctagattg tactgaatta gaagaacata ttcaccaagt tgagaccaaa cagttattac
     2761 aaaagatact gtcgctcccc aattttgatt gtgactttga ggtgactttt gaagatgatt
     2821 accacaaaga gatgaatgat cccctattct acgaatccaa tcttcatcgg atttcggatt
     2881 ttttggaaac tagggatatt aaaaatggtg tagatacact actgacgaaa gacaatcacc
     2941 tggcctttcg tgcctttggt gaaaattatt ctgctagggg aaaggagggc attttaacga
     3001 ctttagtgac ggtcaagtgc tttggtgaag gacggatgcc cattgatatg agtcgctatt
     3061 tctcaactcc tgaaccaaca gttgaaaata gcctaaccct ataaggaggt gcctatgctt
     3121 gaggtatatc taggaaataa tgccaataca aatcaagatt tactaaccat tttgacgacc
     3181 tatggtgtga cttatcgttg tataaaagcg tgtgatgtaa accgtgaaat cttactgtca
     3241 ctctttgcca aaacaatgga ttgttttgag ttattgtcgc cacgatttct tcgctttaag
     3301 cgtcaatatt cgataagttt gaatgagatg attcagctta ttctccaaaa gccagaccag
     3361 aatcttcggt tgccactcat tgtttgtcag aatcatgtct atccagctat tgggctagac
     3421 gaagtgcgaa cctttcttcc cagacaggta aaagaagagc tgtttcaggc cagtctgatg
     3481 aaacaggtga cagggtaggt gtgaagatga atgaacgatt ttgggacaat ttagaaatca
     3541 ttctggcaga aaaagacctc acttgggcag aactagctcg caaagtattc aacggtcaat
     3601 atgtttatcc aagtgagttt aatcgcctct atcaaaaatt acggcattac aaatccaatc
     3661 gtctgatgcc acaaacaaga tgggttgagc gtattgttct agtcttagaa attgattatg
     3721 aagatttatt taagaggtga caatgataag gatagtggtg ttttatctag ctatacagct
     3781 taacggtttt ctattaagtt tatacctaca agagtatatg acgattgagg gtatagtctt
     3841 gctacaattg gtcctattaa gtgtgacttg cttagagatt gcccgtcata aaactgttca
     3901 agcaaaaaat ataaccttga gaaatcgcct aagatggttt cttcttggct ttgtggctat
     3961 ggttgctttt gcagtcttca tcagcttcct atttccaggt cagactagga atcaagcggt
     4021 attggtacaa gtaggaaaac aggttcctcc tattatcttt ttattgtttc tggtcaatgc
     4081 aagtctcctt gaagagattg tttataggca attgctgtgg gaaaaattga cattcccttt
     4141 tgaacaaata ggcgtgacca gttttctctt tgttctgtcc catggaccta atcagttagg
     4201 gagttggctc atgtatagct gtcttggctt gaccttggct gttgttcgat tgaaaactga
     4261 ttgtatgacg gcaatcgcct tacatttact ttggaatagt ttggcttatg tcgtaacttt
     4321 cttgtgatac caaaatcaag agttcttccg tattatggaa gcaccttatg tttgataggg
     4381 aatcgaaaaa aagaggaggt caccatgtca tctgaacaac aagaacgtca agcgatgcaa
     4441 tacgtggaaa gaagtagttt attgacagtc agaaccctct taaagctctt agaatggtcg
     4501 gctaggcaag ccttagctca agattcagct tataagattg gggttcaaaa actggaagaa
     4561 ctccttcaaa gcccttacgc aattgaagcg atagatgtta gtaaagacat actggataag
     4621 ccaatcgatg tggagaaatt taaggagttg atggagtcag aaaaactgcc aatcgcaatt
     4681 agttggcaaa aagattatct ctacttttat gccaaggata agaccttgct cgatcatcac
     4741 ttggatgaat tgttgaagaa actgatgtct aatccagaga agctagaagg tttaatcttt
     4801 gataaaacct tggaccaaga gatagaacta gccaaagaga aaattagagt gacagaacct
     4861 tcggcagtca aaaccaagga ggtgaccttg taatgtattc taggcaaaaa gcatttgtct
     4921 ttggactctt gggtctcgcc tttggctatt tctgccatcg tctaacccta ctctatgata
     4981 gtttaaccaa tgccccacct atggaacgtt ttgcctacct cttaggagag gggggaaatc
     5041 aggttttcaa tcctttatgg ctattttcct ttactcaaaa atctcttctt gcctttatcc
     5101 ttggggtttt aacgatgacg ctagtctatc tttatgtatc gacaggacag aaggtctatc
     5161 gagaagggga agagtacggt tctgcacgat ttggaaacag taaggaaaag cggaactttt
     5221 acagtaagaa tccttttaat gacacgattt tggctcgtga tgttcgtcta accttgttgg
     5281 aaaagaagaa aaaccagttt gaccgaaata aaaatttgat tgtcattggg ggttctgggg
     5341 caggtaagac ctttcgcttt gtgaaaccca accttatcca acttaattgt tccaatattg
     5401 tcgtagatcc taaagaccat ttggctgaga agacaggtaa actcttttta gaaaacggtt
     5461 atcaggtcaa ggttttagac ttggttaata tgaccaattc ggacggtttt aatccctttc
     5521 gttatgtaga aacagagaat gatttgaacc gcatgttaac ggtctatttt aacaataccc
     5581 gtgggtctgg ctctcgcagt gatccatttt gggacgaggc ttccatgaca ttggtgagag
     5641 ctattgcctc ttatttggta gatttttaca atcctccagg aagttccaag caagagcagg
     5701 aagcaagacg taagcgtggc cgttatccag ccttttctga gattgggaaa ctcatcaaac
     5761 tcttatcaaa gggagataat caggacaaaa gtgttcttga agtcctgttt gaagattacg
     5821 ctaaaaaata cggtcatgag aactttacca tgagaaactg ggcagatttt caaaactaca
     5881 aggacaagac cttggattcg gtgattgcgg tcacaacagc taaatttgcc ctctttaata
     5941 tccaatcggt gattgatttg acgcaaagag acactatgga tttgaaaacg tggggcactc
     6001 aaaagaccat ggtatatctt gttattccag acaatgatac gacctttcgt ttcctatctg
     6061 ctttattttt ctctacggtt ttctctactt tgaccagaca agctgatgtg gactttaaag
     6121 gacaactgcc tattcatgtt agaagctatc tagatgagtt tgccaatgtc ggagaaatcc
     6181 cagattttgc cgaacaaacc tcaacagttc gttctaggaa tatgagtcta gtaccaatct
     6241 tgcaaaatat tgcccaactc caaggactgt ataaggaaaa agaagcttgg aaaactatac
     6301 tggggaactg tgacagtctc ctctatttgg gtggaaatga cgaggaaact ttcaaattca
     6361 tgagtggtct tctaggtaaa caaaccattg atgtcagaag taccagtcgt tcttttgggc
     6421 agactggctc aagttctact tctcaccaga aaattgctcg tgacttgatg acggctgatg
     6481 aagtcgggaa tatgaaacga gatgagtgcc tcgtacgaat tgcaggagtg ccagtattta
     6541 gatcaaagaa atattttcca ctcaaacata agaattggaa atggcttgcg gataaggaat
     6601 ctgatgaacg ctggtggcat tatcgcatca ataccctagc tgctgaggaa gagctagatt
     6661 tgtcagggca tacaataagg gatttaagca cagaaacgtc actacattaa tagaaatgag
     6721 gaaaaatatg aatcaaaaac taacaggctt tgtttacgga gtggacgcca gctccatgtt
     6781 ctcccaagcc atgtctctat tacaaaaagg cttaattgca gtaggagcct ttcttgttgt
     6841 catgggtatt atcaaccttt caaccaacat taaagatggt gggccaggtg tccgaaatgc
     6901 cattctagaa attgttggtg gtgtcatggt gggagccgca ggtgcttttg tgacacagat
     6961 taccatttag aggaggtcag aacatgacat gatgatgaat tttacgtcac cttttgtatt
     7021 tctagcctct gaaaaagtgt caagcgatag cctttttgaa gggtttcagg tcgatttaga
     7081 atcaaccgct aacttggtta aatcactggc agactttaac ccgacagttt ggtcttacat
     7141 gacagccatt actaagggga taatgcagcc attgggtgtg gctattctag cagttgttct
     7201 tgtacttgaa ttttcaaaaa tggccaagaa aatcgcaaac tcaggtggtg cgatgacctt
     7261 tgaagccatg gcacctatga ttgtcagcta cattatggtc gcggtcgtga ttaccaatac
     7321 gacggttatt gtggaagcca ttatcgctct tgcctcttat gttattgaac aagtggcagg
     7381 gcttgtcaca aatggtggtg ctaattacga taccatctca ggcatcaagg gatctgggat
     7441 tgtaggaaaa atgattattg gcttttttgc gattctcatt tggttggtga gaatggccag
     7501 tatcatggtt gtcaatatct tgattaccat tcgctttatc caactctatc tgatgattcc
     7561 ttttgcccct gttaccattc caaccttcct tagtgatgac tggagaagtg ttgggattgg
     7621 ctaccttaag aacatcatgg tctatgccgt tcaaggtatt ttgatttttc taattgtatc
     7681 gcttgttccc ttgtttgagt cggctggaaa gattgccgta tcaaatggag ctggagttat
     7741 ggaatcactc gccattatgt ttggtggctt ggtacaggcc atcttgttaa tcattgcctt
     7801 agttggtagt caacgaaccg cccgaagtat tttggggatg taggaggaag ggagtcatta
     7861 tctcgcctcc tctttttata gcttacttag attggagaac atttatgaac actcgtgtct
     7921 ttaaggacat ctcaaaggtt caacacaggg catggctggg atttacaact agacaggtta
     7981 tttttgtatt tccagccatc gccctaacca ttttggtatt gggcttgaac ttattttact
     8041 ggcaatttgg ggattggttt gtctatggtt ttgtttttag ctttaccatt ccactcatgt
     8101 tatttggggt ctatcgccct aatgatttac catttgaaac ctatctcaag taccgatttc
     8161 attatgaact aacagtacca gatcgtacat ttactggaag aaaaggagac caacgtgaaa
     8221 aaattaaaac actcaatgaa accaaagacc tcttctaatg acaaaaagca aaagacgaaa
     8281 gcacagaagc aggagatcaa accctctacc gtaaatacgt tagcctatca agggcttttt
     8341 cagaatggcc tcatgcaggt cagtccaagt tatttctcac aaacctatct cttaggagat
     8401 gttaattatc aaacagttgg cttagatgat aaaggagcta ttgttgagaa atattccgat
     8461 ttaatcaatt cactagatga taagaccaat ttccaactga ccattttcaa tcaaaaggtc
     8521 aatttggaaa aattccgtaa gagtattctc tatcccttac aggaagatgg gtttgatgct
     8581 tatcgtgatg aattgaatcg catgatggat gccaacttag aggcgggtga gaacaacttt
     8641 tcggctgtca agtttctttc atttggcaag agcgaccaaa caccaaaact agcttttcgt
     8701 tctctgtcac agattgggga atatttcaag agtggctttt ctgagattga tgtgtctctt
     8761 ggtctgctcg gtggagagga gcgagtgaat gtccttgcgg atatgttgcg gggtgaaaat
     8821 catttaccgt tctcttacaa ggatttgacc ctgtcaggtc aatccaccaa acactttatg
     8881 gccccaacct acctttcctt taaacacaag aatcatattg aattggacga tcgattatta
     8941 cagattgtct atgttcgtga ctatggtatg gagctagggg ataaatttat tcgtgacctc
     9001 atgcagtcag atttggaagt gatgattagc ctacatgcta aaggctctac taagtctgaa
     9061 accatgacca agctacgaac caagaaaact ttgatggaat cacaaaagat tggtgaacaa
     9121 caaaaaatgg ctcggactgg aatctatttg gagaaggtcg gccatgttct tgaaaacaat
     9181 atcgatgaag cggaggctct tcttcaaacc atgacccaaa caggagataa actgtttgac
     9241 accgtatttc taattggcgt gctggcggat actgaagacc aactcaaaca atctcttgat
     9301 attatcaagc aagttgcagg gtctaatgat atgattattg ataatttgac ctatatgcaa
     9361 gaagctgctt ttaatagtct cttgccattt gggaagaact atcttgaagg tgtttctcgg
     9421 tctctattga cttcaaacat tgccgtgaat gcaccttgga cttccgttga tattcaggac
     9481 aagggtggga aattttatgg catcaatcaa atctcaagta atatcatcag tattgaccgt
     9541 ggcaagttaa atactccgtc aggtttgatt ttagggactt ctggagcggg caaagggatg
     9601 gcgacaaaac atgaaattat ctctactaag ctcaaggaag cagatagtga tactgaaatt
     9661 attattgttg acccagaaaa tgagtacagt attattggtc aagcctttgg tggggagagt
     9721 attgatattg ctccagattc cactaccttc ttaaatgtct tagatctatc tgatgagaat
     9781 atggatgagg atccggttaa ggttaagtcc gaatttctct tgtcttggat tggaaagctc
     9841 ttggaccgca aaatggatgg tcgtgaaaaa tccttgatag atcgtgtgac acgcttgacc
     9901 tataagcatt ttgtcacacc gtccttggtc gaatgggtct ttgtcttagc tcaacaacct
     9961 gaacaggaag ctaaggactt ggcactggat atggaactct atgtggaagg gtcgctggac
    10021 atattttcac atcggaccaa tattaaaaca gacagtcatt tcctgattta caatgtcaaa
    10081 aagttaggcg atgaattaaa acaaattgcc ctcatggtta cctttgacca gatttggaat
    10141 cgggtggtaa aaaatcaaaa actagggaag aagacctgga tttactttga tgaaatgcag
    10201 ctactcttat tggacaagta tgcctctgat tttttcttta aactctggag tcgtgtccgt
    10261 aagtatgggg cgataccgac aggtattact cagaatgtag aaacgcttct tttggatgcc
    10321 aacggcagac gcattattgc caatagtgag ttcatgatac ttttaaaaca ggctaagagt
    10381 gatcgtgaag agttggtgca tatgcttgga ctttctaaag agctggagaa gtacttagtc
    10441 aatcctgaaa aaggggctgg gctaatcaag gccggttcaa ccgttgttcc tttcaaaaat
    10501 aagattccac aacacaccaa gctctttgac atcatgagta cggaccctga aaaaatgagg
    10561 acatgagatg aaagaggata aaaagctagt caaacaggct aggcaaaact ttcgaagcaa
    10621 cttaaaatca gcccgtatgc attatcggaa agaagttaga gccttaaaac agactgttcc
    10681 taaaaaagga agatttcgaa aatcagctca aaattctctg tttcaggaaa agaaaacaga
    10741 gttaaaagaa aatctactca gtagccaaaa ggaagcagaa gagaagttcc taaaggaaat
    10801 tacctatgtt tctcctagac tgttgaaggt aaaggaaatt aagaactatc gacttcctca
    10861 agctcaagaa cgtttgcgga cggcaagaaa acatttgtca gaagtgaaac tgaatgaaaa
    10921 acaacaggca gttaatccca agtttacttt ccaaaaagaa aatccttccc tgaagtctcg
    10981 ctttcagttt caccaagaaa aatcatttga tcgactaagt gcagaaaaag acgtaagttc
    11041 tgccaagcgt gaggttaaac aactcaagaa agtccgaaag tctaagaaaa gttctaccaa
    11101 agtcaaagct ggattaggct tagctgcatc tgaatccctt gacctgatag cacaggatga
    11161 tgatttagat ggacttagaa ccctaacgga tactagcctg aaagtcagac gctatggaag
    11221 gtttaccttt caagcaggta aggtggtagt aaaaagtgga cagactagtg tgcggtttac
    11281 caaaacaaaa ttttctcatg gaaaggaacg attccagaac ttcaaaaagg gaaaaggatt
    11341 cacacgccag aaacctctta aaccaagaag acgctaccaa acctttttaa agcttgccag
    11401 aaaacaaagt gtcgcaggtt tcaaaggaat cgtccaagcc attaagggaa gtatgacttt
    11461 cttttctgtc cttgcgggaa atcctttgac ttggattgtc tcaggcattc tcttgatact
    11521 ccttttgatg atgagctttt tcatgagtgt ctcaggtagc agtgtcattc aacaagatga
    11581 gatggaattg accaaggcct atacccacat aacgtgggaa gatgcggaac atacaagaac
    11641 caacgaaaag ggaattacct tttacactaa gattgatgag gtcatggttt acatgaatca
    11701 tcaataccaa gactacaagc ttgacgattt catggaagca ggtggtgtga cctacaaaga
    11761 atttctcagt caactgtggt cggacttaaa cggtagagat tctattaaat ccatgtctga
    11821 cttgtataag gaacctgctt acaagctgtc tgatgaggac caagaagaac taaaggagtt
    11881 gaccgaagaa ggcaactatt tagcccttca agaattggac aatccctttc agggacagac
    11941 cgatgaggat agcttaaaca tgacctaccg atatggttat gaggtcattg atgagaaacc
    12001 aacgcttcat caccatatca tcttagaagc aaaagaaggt caagtcattg tgtctccgat
    12061 ggatggcaag gtatctcttg atggagagaa tgttattttg atatctggta agggagttaa
    12121 taagactaaa ctaaccttgt ttggcattca ttcaggccga gtgagcgaaa atcaacaagt
    12181 cttggcagga gacattattg gtcaaaccaa ggacggaatg ggtctaaaag tcacctatca
    12241 aaaggttgat gatgacacgg ataagttggt ctatgtcaat ccagcattct actttccaaa
    12301 agtgattcag gttcagacca ccattcttcc aactatcggg cagtttggcg gcgatgagtt
    12361 cgagagagcc aaggcaattt atgactatct taaaagcaaa ggtgcgaccg atcaagctat
    12421 cgcagctatt ttaggaaatt ggtcggtaga atcctctatt aatcccaagc gtgcggaggg
    12481 cgactatcta tctccacctg ttggtgcgac agacggttcg tgggatgatg agggctggct
    12541 ttcactcaat ggtccaacta tatataatgg gcgttaccca aatattctca aacgtggttt
    12601 aggcttagga caatggacag atacctcgga tgggtcacgc agacatactt tattgttgga
    12661 atatgccaaa ggaaaacatc aaaaatggta tgacttaggg ttacaactgg atttcatgtt
    12721 gcatggggat agtccttatt acaccaactg gttaaaggac ttcttcaaaa attcaggaag
    12781 tccagctagt cttgcccaac tatttctcat ttactgggaa ggaaatagtg gtgataagct
    12841 acttgaacgt cagacacgag caagtgagtg gtattaccaa gttgaaaaag gctttagtca
    12901 acccaacggt gggacagcac aaagtgaccc caaagctctt gaagctgtac gaggcgacct
    12961 ctttgaaaac tctattccag gaggtggtga cggtatggga tatgcttacg gccaatgtac
    13021 ttggggagtt gcagcccgta tcaaccaatt ggggctaaaa ctcaaaggta aaaatggtga
    13081 gaagattcca attatcagta ccatgggcaa tggccaagat tgggtacgaa cagccgcaag
    13141 tctcggtgga gagacaggga caagtccaca agcaggagct atcctttcct ttgcgggagg
    13201 aggacatggc acaccaacaa aatacggaca tgtggctttt gtggagaaag tctacccaga
    13261 tggctcattc cttatctccg aaaccaacta caatggcaat ccaaactata ccttccgtaa
    13321 attatctgga gtggatagta gcttgagttt tgcttatacg acgaaataaa aaaggatatg
    13381 ctaacaaaat gaataaatgt tagcatatcc ttttttattt tttgtcacaa ttaatcagaa
    13441 tttatagaaa tgagtactgt tataagacga atcatctcat caagaacatc tg
//
LOCUS       DBNFEG010000040        13402 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.040, whole genome shotgun sequence.
ACCESSION   DBNFEG010000040 DBNFEG010000000
VERSION     DBNFEG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13402
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.040"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..1050)
                     /locus_tag="AC1PNG_000816"
     CDS             complement(<1..1050)
                     /locus_tag="AC1PNG_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy0128 family protein"
                     /protein_id="HIP6701331.1"
                     /translation="MNTKKWRTSLLIPGIVLFGTVALVNNVSAQEVKNTIISAKQPDG
                     RQATSKAVNVKIPAVVRLFGRELLENEFKFELREANSEKLIDTTQNTKEGQVSFKNLS
                     FNKPGEYWYTISEVKNELSGVEFDSKYIVAKITVEDRNNGQLQAMIEFIADDNVFNNF
                     YTPAPAAASLSIKKVLEGRTLNTSEFEFVLKNEKGDEIEKVSNQADGSVNFSALTFTK
                     EGTYTYTVSEVDGGLGDIIYDKSDIKATVTVKDNNHGQLVSTVTYENSDQIFENILNP
                     GKLITPTTDSVITDNEVSKEAMTDKEKGNIEPPKEQIATEEKDNIEASEKQMPSIVND
                     MVVTPEKQMTNKENDN"
     gene            1360..2358
                     /locus_tag="AC1PNG_000817"
     CDS             1360..2358
                     /locus_tag="AC1PNG_000817"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIP6701332.1"
                     /translation="MKVLLYLEGKTVLEKSGIGRALQHQMEALDMAGIPYTTDLLGDY
                     DVIHINTYGPRSWLLLHAAKRSGKKVIMHGHSTKEDFQNSFIGSNTLAPFVGKYLASM
                     YQKADFVITPSEYSKKLIQSYGVTTPIIAVSNGIDLDKYKKDERKEAIFREHFKIEEG
                     QPVVVCAGLYFRRKGIEDFVKVAEKMPHVRFIWLGSINKWLIPSYIRKIVEGDHPANV
                     EFPGYFKGAVFQGAMSGADAFFFPSYEETEGIVVLEALASHQHVVLRDIPVYDGWVDE
                     NVAELGHTVDDFVDSIQKILDKQVDKREAGYQVAVSRSIDNVSHQLVDAYRQVLEM"
     gene            2359..3672
                     /locus_tag="AC1PNG_000818"
     CDS             2359..3672
                     /locus_tag="AC1PNG_000818"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIP6701333.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKLGHTVFIFTTTDED
                     VNRYEDWDIIRIPSVPFFAFKDRRVAYAGFTDALKIASRYKLDIIHTHTEFSLGILGK
                     MIAKELDIPVVHTYHTQYEDYVHYIAKGRIIRPGMVKYLVRAFLKDLDGVICPSEIVE
                     DLLMSYNVPISKRVIPTGIDLAKFDRPEIGKDETDELREQLGIASDETMLLSLSRISH
                     EKNIQAIVQAMPKILLENPKVKLVIVGGGPYTDDLQEMIAELNLDEHVQLTGMIAPSD
                     TALYYKAADFFISASTSETQGLTFLESLASGTPILAHSNPYLKNVVTDKMFGTLFVRE
                     QELADAVVEAIVATPPMNPYVLDKKLYEISSTNFGRKVFEYYLDLKISYDFRKEHTNQ
                     DSVAEALIKEAIYLPQKVVVKSTDTTAKILKKSVEQVKSIRNFFE"
     gene            3687..4061
                     /locus_tag="AC1PNG_000819"
     CDS             3687..4061
                     /locus_tag="AC1PNG_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701334.1"
                     /translation="MEKLRKESWKNLVLATLIILSMYAGIYLIAFHLISDTMLSKELV
                     VGNILLYTPIVLFLTGLLYGFFHGFSPSLLALVLLLYPVTIFFFKEWIILYQIVYTLC
                     ALIGNGVGALLFSLRKKMRKNP"
     gene            4325..4696
                     /locus_tag="AC1PNG_000820"
                     /pseudo
     CDS             4325..4696
                     /locus_tag="AC1PNG_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
     gene            4714..6663
                     /gene="thrS"
                     /locus_tag="AC1PNG_000821"
     CDS             4714..6663
                     /gene="thrS"
                     /locus_tag="AC1PNG_000821"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIP6701335.1"
                     /translation="MIKITFPDGAVREYQAGVTTFEIAESISKSLAKKALAGKFNGKL
                     IDTTRAIEEDGTLEIVTPDHEDALDILRHSAAHLFAQAARRLFPDIKLGVGPAIQDGF
                     YYDTDNAAGQISNEDLPRIQEEMMKIVKENFPSERREVTKEEAIEIFKNDPYKLELIQ
                     EHSDDEGGLTIYTQGEYIDLCRGPHVPSTGRIQIFELLNVAGAYWRGKSENPMMQRVY
                     GTAWFDKKDLKAYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRT
                     LERYITDKELTSGYQHVYTPPLASVELYKTSGHWEHYSEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVYKNHVRSYRELPVRIAELGMMHRYEKSGALTGLQRVREMTLNDGHIFVTP
                     EQIQEEFKKALQLIIDVYADFNLNDYRFRLSYRDPEDKEKYYDNDEMWENAQRMLKGA
                     MDEMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFGLTYIGADG
                     EEHRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPTQVTMIPISVEAHLDYAWKVA
                     KELQDRGVRVHVDERNEKMQYKIRQSQTSKIPYQLIVGDKEMEDNAVNVRRYGSKATQ
                     TQSVAEFVDHILADIARKSRPAEAE"
     gene            complement(6735..7394)
                     /locus_tag="AC1PNG_000822"
     CDS             complement(6735..7394)
                     /locus_tag="AC1PNG_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIP6701336.1"
                     /translation="MNYVLELLKPSTFQLLWNGLKLTLYISSISVILSILFGMILAIM
                     RNGKNPIFKWIATIYIEFVRNVPNLLWIFTVFLVFQMKSTPAGITAFTIFTTAAMAEI
                     IRGGLNAIGPGQTEAGLSQGFTPVQIMVYIIMPQAIRKMLPAIISQIVTVIKDTSFLY
                     SVIALQELFGSSQILMGRYFEAEQVFALYLMVALIYFSINFAISSLSRYVAKSWASSI
                     E"
     gene            complement(7409..8107)
                     /locus_tag="AC1PNG_000823"
     CDS             complement(7409..8107)
                     /locus_tag="AC1PNG_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIP6701337.1"
                     /translation="MTVLTTSPYAWENWVSYFKDFPTFFQGFLFTLAISVGAFIMAMF
                     LGIVFGSLSSSKNKVLKIIARVYVEYYQNTPLLVQFMIVYYGLPLISNYVLIPSIYWT
                     AVICVGLYHGAYIAEVIRAGIEAVPTGQTEAALSQGFTQSETMRIIILPQAIPTILPP
                     LTNQVVNLIKNTATVAIISGADIMFMTKSWSAMNANYIPAFAGAAFLYFIMCFPVASW
                     GRRIEEKNKSAFSH"
     gene            complement(8122..8961)
                     /locus_tag="AC1PNG_000824"
     CDS             complement(8122..8961)
                     /locus_tag="AC1PNG_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIP6701338.1"
                     /translation="MDKKKINIFFAIRLLVFGLILFVFAKQPVAQTETPASITNSDQV
                     QAIIDRGVLRVGVKQDVPNFGYKNPDTGEFEGMEIDIARKIADELGVDIEFTPVTAQT
                     RGPLLDNGQVDMVIATFTITEERKLLYNFTTPYYTDAVGFLVNKDSGIKTFTDLDGKT
                     IGVAQGSITRTLISKLADKYGISVNFAELGSYPELSVSLRAHRTDAFSVDQSILSGYI
                     SSKSELMDFSFSASDYGIVTKLSNKDLNNYLNSLVEKWTADGSLQSIYDANGLKPVTE
                     TDE"
     gene            complement(8972..9733)
                     /locus_tag="AC1PNG_000825"
     CDS             complement(8972..9733)
                     /locus_tag="AC1PNG_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIP6701339.1"
                     /translation="MALVEFKDVNKYYGDYHALRNINLEFEPGQVVVLLGPSGSGKST
                     LIRTINGLEAIDEGTLIVNGHDISSTSIKELVSLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKATAEKTAQKYLEFVNLWDRKDSYPAMLSGGQKQRVAIARGLAMKPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDGMNMIVVTHEMGFAREVADRIIFMAEGEVL
                     VDTTDVNGFFDNPTEPRAQQFLSKIINHESDKVKL"
     gene            9927..10631
                     /gene="yycF"
                     /locus_tag="AC1PNG_000826"
     CDS             9927..10631
                     /gene="yycF"
                     /locus_tag="AC1PNG_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HIP6701340.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTREGYEVVTAFDGREALEVFEAE
                     FPDIVILDLMLPELDGLEVARTIRKTSNVPILMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELQARVKALLRRSELAETQTNIESTGTPELVIGDLVILPDAFVAKKHGKELELT
                     HREFELLHHLAKHLGQVMTREHLLETVWGYDYFGDVRTVDVTIRRLREKIEDTPSRPE
                     YILTRRGVGYFIKGND"
     gene            10624..11973
                     /gene="vicK"
                     /locus_tag="AC1PNG_000827"
     CDS             10624..11973
                     /gene="vicK"
                     /locus_tag="AC1PNG_000827"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HIP6701341.1"
                     /translation="MINQLRYLMTTAEFWFVVILIGFLIALTVLLIENYRDNKQIKQL
                     NQKVNALIEGNYADVLDMRGSPEITDMANSLNDLSEVIRLTHDNLEQEKTRLTSILSY
                     MSDGVIATDRIGRIIMINDMAQKQLGLSSQKQEQYHLLEVLDLSDRYTLRDLLAQTPE
                     IVIDHTNENEEFLTLRANFATIRSESGLISGLVVVLHDMTEQAKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALTESVAPSFVKVSLDETNRMMRMITDLLSLSRIDNQVG
                     QIDVELINFTAFVTFILNRFDQMKNTDSDKVYTIVRDYQISPIWVEIDTDKMTQVLDN
                     ILNNAIKYSPDGGTITFSMKTTDSQLIVSVSDEGLGIPKTDLPRIFDRFYRVDKARSR
                     AQGGTGLGLAIAKEIVKQHKGFIWAKSEYGHGSTFTIVLPYSKDIALDEWDDSDEEE"
     gene            11980..12783
                     /locus_tag="AC1PNG_000828"
     CDS             11980..12783
                     /locus_tag="AC1PNG_000828"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIP6701342.1"
                     /translation="MIGKGFNYSILASGSSGNCFYLETDKKKILVDAGLSGKKVTSLL
                     AEIDRKPEDIDAILVTHEHSDHIHGIGVLARKYGMDIYANELTWQAMESKLGKIDVAQ
                     KHIFELGAMKTFGDLDIESFGVSHDAACPQFYRFMKDDKSFVMLTDTGYVSDRMVGIV
                     ENADAYLIESNHDIEILRSGSYSWNLKQRILSDKGHLCNEDGADAMIRSLGNRTKKIY
                     LGHLSKENNIKELAHMTMVNQLAQADLGVGVDFQVYDTSPDTATPLTKI"
BASE COUNT         3902 a         2486 c         3038 g         3976 t
ORIGIN      
        1 attatcgttc tctttattag tcatttgctt ttcaggtgtt acgaccatgt cgttcacaat
       61 gcttggcatc tgtttttcag aggcttcaat attatccttc tcttcagtag ctatttgctc
      121 tttagggggt tcgatatttc ccttctcttt atcggtcatt gcttccttag agacttcatt
      181 atcagtaata acgctatccg tggttggcgt tattaacttc ccaggattca aaatattctc
      241 gaagatttga tcgctatttt cataagtcac tgttgagact agttgtccgt gattgttatc
      301 tttcacagtg acagtggcct taatatctga tttgtcatag ataatatcgc caagtccacc
      361 atcaacttct gaaacagtgt aggtataggt tccctctttt gtaaatgtta gggcactaaa
      421 gtttacagaa ccatctgctt gattgcttac cttttcgatt tcatcgcctt tttcattttt
      481 taaaacaaat tcgaattcac tggtgtttaa ggtacgtccc tcgaggactt ttttaatcga
      541 aagactagca gcagctggag caggtgtata gaaattgtta aagacattgt catcagcaat
      601 aaattcaatc attgcctgta attgtccgtt gtttcgatct tctacagtta tttttgctac
      661 aatatatttc gaatcaaact caacaccact aagctcattt tttacttctg aaattgtata
      721 ccaatattcg ccgggcttat tgaatgatag atttttaaaa ctaacttgac cctcttttgt
      781 attttgagtt gtatcaataa gtttctcact attcgcttct ctaagctcaa atttaaattc
      841 attttctaga agctcacgac caaatagtcg tactactgct ggtattttga cattaaccgc
      901 ctttgaagta gcctgtctcc catcaggttg ttttgcgctg atgatggtat tttttacttc
      961 ttgtgccgat acattattca ctagagcaac agttccaaat aatactattc ctggtattag
     1021 gagcgatgtt ctccatttct tagtattcat tatccttttt cctttctcaa agcatgattt
     1081 atgtagctaa tctgtgtcgt tataacatgc aaattttaca tctaaagtcc tcaataaatt
     1141 ttgaacgctt agtgccagaa tgtgcaccat gcgtaaatta taacatcttc taaaaaacat
     1201 tcaagctgtc cttctgtttt taaaatctgt tttatctgtt ttttatgtaa tcatatattt
     1261 gggattatat tctttagtat tggctttaat tgatacaaaa ttcgtgtaga atacagttta
     1321 aaatatgaaa aaaatgtaaa aaagggctat aatggtatta tgaaagtact attgtattta
     1381 gaaggtaaaa ctgttttaga gaaatcagga attggccggg cacttcaaca ccagatggaa
     1441 gcgctagata tggcaggcat tccttataca acagacctgt tgggagatta cgatgtcatt
     1501 catatcaata cctatggtcc acgctcctgg ttattgctac atgcagccaa acggagtgga
     1561 aaaaaggtaa tcatgcatgg acattccacg aaggaagatt ttcaaaattc ctttatcggt
     1621 tcgaataccc tagctccttt tgtagggaaa tatctagcaa gcatgtacca aaaggcagac
     1681 tttgtcatca ctccttcaga gtattcaaaa aaactcatcc agtcttacgg tgtgacaacg
     1741 ccaattatag ccgtttcgaa tggtattgat ttagataaat ataagaagga tgaacgcaag
     1801 gaagcaatct ttcgagagca ttttaaaatt gaagaagggc agccggttgt tgtctgtgca
     1861 ggactctact tccgacgtaa aggaattgaa gactttgtca aggtagccga aaagatgccg
     1921 catgtccgct tcatttggct cggttcaatc aataagtggc tgattccgtc atatattaga
     1981 aaaattgttg agggagatca tccagcgaat gtcgagtttc caggttattt taaaggtgct
     2041 gtttttcaag gtgcaatgag cggagcggat gcctttttct tcccatcgta tgaggaaaca
     2101 gaagggattg ttgttctgga agctcttgct agtcatcagc atgttgttct aagagatatt
     2161 ccagtttacg atggttgggt tgacgaaaac gttgccgaat tgggacatac agtggatgat
     2221 tttgtcgatt ctattcagaa aatcttagac aagcaggttg ataaacgtga ggcgggctac
     2281 caggttgcgg ttagtcgctc gattgacaat gtttcccacc aattggtgga tgcctatcga
     2341 caagtattgg agatgtaaat gcggattgga ttgtttacgg atacctattt tccacaggtt
     2401 tcaggagtcg cgacttcgat tcggactctt aagacagagt tggagaaatt gggacatacg
     2461 gtatttattt ttacgacgac agatgaggat gtgaatcgct acgaagattg ggacattatc
     2521 cgaattccta gcgtaccttt ttttgccttt aaagatcgtc gagtggccta cgcaggtttt
     2581 acagatgcct taaaaattgc aagtcgctat aaactagata ttattcatac tcatacagaa
     2641 ttttctttgg ggattttggg gaaaatgatt gccaaggaac tagatattcc ggtggtacat
     2701 acttatcaca cccagtatga agactacgta cactatattg ccaagggtag gattattcgt
     2761 cccgggatgg ttaaatattt agtacgggca tttttaaaag atttggatgg ggtaatttgt
     2821 cctagtgaga ttgttgaaga tcttttaatg agttacaatg ttccgatttc taaacgagta
     2881 attccgacag gaattgactt ggcaaagttt gatagaccag aaattggcaa ggatgaaaca
     2941 gatgagttgc gggaacagct aggaattgct agtgatgaaa cgatgttgtt aagtctgtct
     3001 aggatttctc acgagaagaa tatccaagca atcgttcagg caatgccaaa aattttgcta
     3061 gaaaatccaa aggtgaagtt ggtcattgtc ggaggtggac cttatacaga tgatcttcag
     3121 gaaatgatag cggagcttaa tctagatgaa cacgtccagt tgacgggaat gattgcacca
     3181 agtgatacag cactttatta taaagctgcg gatttcttta tttctgcttc gaccagtgaa
     3241 acacagggac taactttctt agaaagttta gcttctggaa ctcctatttt ggcacatagc
     3301 aatccctact taaaaaatgt tgtaacagat aagatgtttg ggacactctt cgttcgagaa
     3361 caggagttgg cagatgccgt cgttgaggcg attgttgcta caccaccaat gaatccgtat
     3421 gtgcttgata agaagctcta tgagatttct tctacgaatt ttgggcgcaa ggtttttgaa
     3481 tattatctag atttgaagat ttcgtatgat tttagaaaag aacatacgaa tcaagatagc
     3541 gtagcagagg cattgataaa agaggctatt tacctaccgc aaaaagtagt agttaaatca
     3601 acagacacga cagcaaaaat attgaaaaaa tcagtcgaac aagtaaaatc aatccggaat
     3661 ttctttgaat aatttggagg tttcaaatgg aaaagcttcg gaaagagagt tggaagaatc
     3721 tggttttggc aactcttatt attctgtcca tgtatgcagg tatttatctg attgcctttc
     3781 atctgattag tgatactatg ctttctaagg aactggtggt tgggaatata ttgctctata
     3841 ctcccattgt actattccta acaggtcttc tttatggatt ttttcacggt ttttctccta
     3901 gccttcttgc cttggtgtta ctgctttatc ctgtaaccat ctttttcttt aaggaatgga
     3961 ttattctcta tcagattgtc tacaccttat gtgctctgat tggaaatggg gtaggagctc
     4021 tgctattttc tctaagaaag aaaatgagga aaaatccttg atatatcgga aaatccttgt
     4081 tataatagct tttagagact tatcaattct aggaaatctt tgagagagtg tgcggtgggt
     4141 gcaagcacat agaggatagg attgacacta ctcagtatga ttttatgcaa acataaaagg
     4201 gtggttccct tatcaccaat ctgaggtagt caggcttttt agcgtgactg cgaatgaagg
     4261 tggaaccacg ttacgacgtc cttttgtgag ggcgtcgtat tttctttttt gacaaaacaa
     4321 ggagatgaag ttgaccagca ttccacaaga aacgattgat cgttgcctag ccgttcgcaa
     4381 ggcttacaga gagttggaac tcaaacacca cgacaaagag tggtccattg aggagtgaaa
     4441 cagtctgggg atagactgtt tcagctcaac aacttaaaat aaagacttgt tgaccaatga
     4501 tattggcaac atgaaccgtc tgattatgac caagcagggt cgttactatg atgaaacgcc
     4561 ttataccttg gagcataaaa tggctgaaaa tatctggtgg ttgattgaac tggctgaccg
     4621 cctggatatt gatattcaaa aagaaatgga aaccttttta actcagaaag aagagttatt
     4681 ggggattaaa aaataaataa aaaggagata aacatgatta aaatcacttt cccagatggt
     4741 gctgttcgtg agtaccaggc tggcgtgaca acttttgaaa ttgcggagag tatcagcaag
     4801 tctttggcga aaaaggcgct tgctggtaag ttcaatggta aattgattga caccactcgt
     4861 gccattgaag aggatggcac gcttgaaatc gtaacacctg accatgagga tgccttggac
     4921 atcttgcgtc actcagcagc tcacttgttt gcccaagcgg ctcgtcgcct tttcccagac
     4981 atcaagttgg gggttggtcc tgctattcag gatggtttct actatgacac ggacaatgcg
     5041 gctggtcaga tttcaaatga agacctgcca cgcattcagg aagaaatgat gaagattgtc
     5101 aaggaaaact tcccttcaga acgtcgtgag gtgaccaagg aagaagctat tgagattttc
     5161 aaaaacgatc cttacaagtt ggaattgatt caagaacact ctgatgacga gggcggtttg
     5221 accatttata cccagggcga atacattgac ctctgccgtg gcccacacgt tccgtcaaca
     5281 ggtcgtatcc agatttttga attgctcaat gtggctggtg cttactggcg tggtaagagc
     5341 gaaaatccaa tgatgcaacg ggtttatggt acagcttggt ttgacaagaa agaccttaag
     5401 gcttaccttc aaatgcgtga agaggcaaaa gaacgtgacc accgtaaact tggtaaggaa
     5461 ttggatctct tcatgatcag ccaagaagtt ggtcaaggct tgccattctg gttgccaaat
     5521 ggagcgacca tccgtcgtac cttagagcgt tacatcacag ataaagagtt gacttcaggt
     5581 taccagcacg tttatacgcc accattggct tctgttgaac tctataagac ttcgggtcac
     5641 tgggagcatt attcagaaga tatgttccca actatggaca tgggcgacgg tgaagagttt
     5701 gttcttcgtc cgatgaactg tccgcaccac attcaagttt ataaaaatca tgtgcgttca
     5761 taccgtgaat tgccagtgcg tatcgccgag cttggtatga tgcaccgcta tgaaaaatct
     5821 ggtgcattga ctggtttgca acgggtgcgt gagatgaccc tcaacgacgg tcatatcttt
     5881 gtcacacctg agcaaatcca agaagagttc aaaaaagctc tccagttgat catcgatgtg
     5941 tacgcagatt tcaacctaaa tgactaccgt ttccgcctgt cttatcgtga cccagaggac
     6001 aaagagaagt actatgacaa cgatgagatg tgggaaaatg cccaacgcat gttgaaaggt
     6061 gccatggatg aaatgggcgt agactacttt gaggcagagg gtgaagctgc cttctacggt
     6121 ccgaaattgg atattcaagt gaagactgcc cttggtaacg aagaaaccct gtcaactatt
     6181 cagttagact tcctcttgcc agaacgcttt ggtttgactt atatcggtgc tgatggtgag
     6241 gagcaccgcc cagtcatgat tcaccgtggt gttatctcaa ccatggaacg cttcacggct
     6301 atcttgattg aaacctacaa gggtgccttc ccaacttggt tggctccaac ccaagtgact
     6361 atgattccga tctctgtgga agctcacttg gactacgctt ggaaggttgc taaggaattg
     6421 caagaccgtg gcgttcgcgt acacgtggat gagcggaacg agaagatgca atacaagatc
     6481 cgtcagagcc agaccagcaa aattccatac cagctcatcg tcggtgacaa ggaaatggaa
     6541 gacaatgctg tcaacgtccg tcgctacgga agtaaggcaa cgcaaactca gtctgtcgca
     6601 gaatttgtgg accatatcct agcagatatt gcccgcaaat cacgtccagc tgaagctgaa
     6661 taaataaagt aagaagtccg aaaaggcttc ttttttgaca gcaaaaactc ccaaccaact
     6721 ggctgggaga aattttattc tatactgctc gcccaagact tagccacata acgtgacagg
     6781 cttgaaatgg caaagttgat gctaaagtaa atcaaggcta ccatgagata gagtgcaaaa
     6841 acttgttctg cctcaaagta gcgtcccatg aggatttgac tcgaaccgaa gagttcttgc
     6901 agggcgatga cggagtagag gaaactagtg tccttgatga ccgttacaat ctgcgagatg
     6961 atggctggca gcatcttgcg aatggcctgc ggcatgatga tatagaccat gatttgcact
     7021 ggggtaaaac cctgtgacaa gccagcttcg gtctggcccg gtccgatggc gtttagacca
     7081 ccacggataa tctcggccat ggctgccgtg gtaaaaatag taaaagcagt aataccagca
     7141 ggcgttgact tcatttggaa gactaagaag acggtgaaaa tccagagcaa gtttggtaca
     7201 tttcgcacaa actcgatata gatagttgca atccacttga aaattggatt ttttccattg
     7261 cgcatgatgg cgagaatcat tccgaaaaga atggacagga ttaccgaaat gcttgagata
     7321 tagagtgtta acttcaaacc attccacaag agttggaagg ttgaaggttt gagtaattct
     7381 aaaacataat tcatcgttca acctcctttt aatgtgaaaa ggcagactta ttcttttctt
     7441 caatgcgacg tccccagctt gcaacaggga agcacatgat gaagtaaagg aaagcagcac
     7501 cagcaaaggc tgggatgtag ttggcattca tggcagacca agattttgtc ataaacataa
     7561 tatcagctcc agagataatg gcaacagtag ccgtattctt aattaagttt actacttggt
     7621 tggtcaaagg tggaagaatg gtcggaatgg cctgtggcaa gatgatgatt cgcatggttt
     7681 cagactgagt aaatccttgt gagagtgcgg cttcagtttg cccagtaggc acagcctcaa
     7741 tccctgcacg aatgacctct gcgatatagg caccgtgata gagtccgacg cagataacag
     7801 ctgtccagta gatggacggt atgagaacat aatttgaaat cagcggaagt ccataataaa
     7861 caatcatgaa ctgaaccaaa agcggcgtgt tctgatagta ttcaacatag acacgcgcga
     7921 tgatttttaa aactttattt ttacttgaag agagactacc aaagacaatt cctaaaaaca
     7981 tggccatgat aaaggcaccg acagaaatgg ctaaggtaaa gagaaaacct tggaaaaagg
     8041 ttggaaaatc tttgaaataa ctgacccaat tctcccaagc gtaaggacta gttgttaaaa
     8101 ctgtcatatt gtttcacctt tctattcgtc agtttctgtg actggtttga gaccattggc
     8161 atcatagatg ctttgtaggc taccatcggc agtccatttt tctacgagac tatttaagta
     8221 gttgttaagg tctttgttag ataatttagt aacaattcca tagtcagaag ctgagaagct
     8281 aaagtccatg agttcagatt tagagctgat atacccagaa aggatagatt ggtctactga
     8341 aaatgcgtct gtacggtgag cacgtagcga gacagagagt tctggataag aaccaagctc
     8401 tgcaaaattg acagagatac catatttgtc tgctagttta gagatgaggg tacgtgtaat
     8461 ggaaccctga gctacaccga ttgtctttcc atccaggtcg gtaaatgtct taattccact
     8521 atccttattg accaagaagc caacggcatc tgtgtagtaa ggagttgtga aattgtagag
     8581 gagtttacgt tcctcagtga tagtgaaggt tgcgatgacc atatccactt gaccgttatc
     8641 taagagtggt cctcgggttt gcgctgtaac gggagtaaat tcgatatcta cacctaattc
     8701 atctgcaatt ttacgagcaa tatcaatctc cattccctca aattcaccag tatcagggtt
     8761 cttgtagcca aagtttggga cgtcctgttt cactccgact cggagaacgc ctctatcgat
     8821 gatagcttga acttgatcac tattggtaat ggatgcgggt gtttctgttt gagctactgg
     8881 ttgttttgcg aaaacgaaaa gaataagtcc aaagaccaat aatctaattg caaagaaaat
     8941 gttgattttt ttcttatcca tagacttcct cctagagttt tactttgtca gattcgtggt
     9001 tgataatctt gctgaggaat tgttgggcac gaggctctgt cggattgtca aagaagccat
     9061 ttacatctgt tgtatcaact aaaacttctc cctcagccat gaagatgatg cgatcagcta
     9121 cttcacgggc aaagcccatt tcgtgtgtta cgacaatcat gttcatcccg tcacgtgcca
     9181 atttttgcat aactgcaagt acatcgccga tagtttctgg gtccaaagca gatgttggtt
     9241 catcgaagag tagaagctca ggtttcatag ctaagccgcg agcgatggcg acacgttgtt
     9301 tttggcctcc ggatagcata gcagggtagg aatccttccg atcccagagg ttaacaaact
     9361 cgagatactt ttgagcagtt ttttcagccg tagctttatc gattcccaaa accttaatcg
     9421 gtgccaaagt aacattttct aacacggttt tgtgtgggta aaggttgaaa tgctggaaaa
     9481 ccatgccaac ttctttgcga agtgatacga gctctttgat agaagtagag gaaatatcat
     9541 gtccattgac gatgagcgtt ccttcatcaa ttgcctctaa accgttaatc gtgcgaatga
     9601 gagtagattt tccggatcca gaaggtccaa ggagaacgac aacctgtcca ggttcgaatt
     9661 ccaagttgat attgcgtagg gcgtggtagt cgccatagta tttgttgaca tctttaaatt
     9721 ctactaaagc cataaaattc tccttgcata gtgtttttta ggtattccca tcttagcatt
     9781 gcctatatac aatgtcaagt aaattctgac aatttaatgt attgtatgta ataaaatatg
     9841 acatagctgt gtcagatggt tttgaaatat gaatttttta ggtaagtctg atataataga
     9901 agaaataaag caaaggtaag atactaatga aaaaaatatt aattgtagat gatgaaaaac
     9961 caatctcaga tattattaag tttaatatga cgcgtgaggg atatgaagtt gtgacagctt
    10021 tcgatggacg tgaagccttg gaagtgtttg aggctgagtt tcctgacatt gtcattttgg
    10081 acttgatgtt gccagaattg gacggactag aggttgctcg aaccattcgt aagaccagca
    10141 atgttccaat cttgatgtta tctgctaaag atagcgaatt tgataaggtt atcgggcttg
    10201 aaatcggggc ggatgattat gtgaccaaac ccttctctaa tcgcgaattg caggcgcgtg
    10261 ttaaggctct tcttcgtcgt agtgaattgg cagagacgca gacaaatatt gagtcaacag
    10321 gaactccaga gttggtgatt ggcgatttgg tcattctgcc tgatgcgttt gtcgctaaga
    10381 agcatggcaa agagctggag ctgacccatc gtgagtttga attgctccac catctggcca
    10441 aacacttagg tcaggttatg actcgagaac atctattgga aacagtttgg ggttatgatt
    10501 actttggtga tgtccgcacg gtggatgtaa cgattcgtcg tctgcgtgag aaaattgaag
    10561 atacaccaag cagaccagaa tacattctta ctcgtcgcgg agtgggatat tttataaaag
    10621 gaaatgatta atcaattacg ttatttaatg accacggcag aattttggtt tgttgtcatt
    10681 ttgattggct ttctaattgc tctgacggtc cttttgattg aaaactatcg ggataataag
    10741 caaattaaac aactcaatca aaaagtcaat gccttaattg aggggaatta tgcagatgtg
    10801 ttagacatga ggggaagtcc tgaaatcaca gatatggcga attctcttaa tgatctgtcc
    10861 gaagtcatcc gcttaactca tgataattta gaacaagaaa agacgcgtct gacttccatc
    10921 ctgtcctata tgagtgacgg tgttattgct acagaccgta tcggacggat catcatgatt
    10981 aacgatatgg cccagaaaca gttggggctt tctagccaaa agcaggagca gtatcacctg
    11041 ctcgaggttt tagatttatc agaccgttat acattgagag atttgttggc tcagacacct
    11101 gagattgtga ttgatcacac caatgaaaac gaagaatttt tgactttacg tgctaatttt
    11161 gccacgattc gtagtgagag tggcctgatt tctggcttgg tagtcgtctt gcatgatatg
    11221 accgagcagg ccaaggaaga acgggaacgt aggctgtttg tgtcaaatgt gagtcatgaa
    11281 ttgcgtacgc cattgacttc cgttaaatcc tatctagagg ctctggatga gggggcgctg
    11341 acggagtctg ttgcaccgag ttttgttaag gtatccttag atgagaccaa ccgcatgatg
    11401 cggatgatta cagacctcct aagcttatcg cgtattgata atcaagttgg tcagatagat
    11461 gttgaactga taaactttac tgcttttgtg acctttattc ttaaccgttt tgaccaaatg
    11521 aaaaatactg attctgataa ggtctatacg attgttcgtg actatcaaat cagtcctatt
    11581 tgggttgaga ttgatacaga taagatgact caggttttag ataatatctt aaataatgcc
    11641 attaaatatt cgccagatgg cgggacaatt accttcagca tgaagaccac agatagccag
    11701 ttgattgtat ccgtatcaga cgaaggtctg gggattccga aaacagattt acctagaatt
    11761 tttgaccgat tttatcgcgt agacaaggca cgatctcgtg cccaaggcgg tacaggtctt
    11821 ggtttagcta ttgcaaaaga aatcgtgaaa caacataagg gctttatctg ggctaaaagc
    11881 gaatatggtc atggctcaac gtttacaatt gtcttgccgt acagcaagga catcgcactg
    11941 gatgagtggg atgattcaga tgaggaagaa taggagaata tgatcggaaa aggttttaat
    12001 tatagtattt tagcttcggg gtccagcggg aattgttttt acctagaaac agataaaaag
    12061 aaaattttag tggatgctgg cttatcaggg aaaaaggtta ccagtctttt ggcggaaatt
    12121 gatcgaaagc ctgaggatat tgatgctatc ctggtaacgc atgaacatag cgatcacatc
    12181 catggtatcg gtgttttagc acgtaagtat ggcatggata tttatgccaa tgaattgacc
    12241 tggcaggcta tggagagcaa attgggcaag attgatgtgg ctcaaaaaca tatcttcgaa
    12301 ttgggtgcta tgaagacttt tggtgaccta gatattgagt cctttggagt tagccatgat
    12361 gctgcatgtc cgcaatttta ccgttttatg aaggatgaca aatcctttgt tatgttgacg
    12421 gatacaggct atgtcagtga ccgcatggtt ggaattgtag aaaatgccga tgcttatttg
    12481 attgaatcga accatgatat tgaaattttg cgctcaggtt cttattcatg gaatttgaag
    12541 caacggattc tatccgataa gggacatctt tgtaacgaag atggagctga tgccatgatt
    12601 cgctcgttgg gaaatcggac caaaaagatt tacctagggc atttgtcaaa ggaaaacaat
    12661 atcaaggaat tggctcacat gaccatggtt aatcaattgg ctcaggctga tttaggagtt
    12721 ggagtggatt tccaagtcta tgacacatcg ccagatacag cgactccctt gaccaagatt
    12781 tgacaattga tacaaactaa gttatactga aattactaat tatacgaggt gaagttatat
    12841 gaataattaa gctgcttatt agtcgttctt aaaacttgta gtgaggttgt attaaccgat
    12901 gatacaaggg ataagactgc tcataagggc ttactattcg tgtgactttt tccataggga
    12961 aaggtcatgt tttgcatggc ttttttcttg ttcattgagc agaatagggg ccctttttgt
    13021 gaagaaaaga ggtctatatg gaagtaataa aatgtattcg attagagaag gaatttgcgg
    13081 gatgtagagg ctgggcaaaa agcttaacct cgcttctcag ggttcgtgtc aacatctcag
    13141 cgcagtggtt gattggcaga tttgttcgtg ttttacactc caaatctgac cttacgatcg
    13201 ttgcgaacaa agttcgcttt atctccaacc tcaaactgtc tcccagacag tttgagctgt
    13261 gcgggggtgg gagtgaaaca gtctggggat agactgtttc agctcaacaa ctggaaataa
    13321 ggacttgttg acgaactctt ttttctacca tttgtcaagt ccatcaaggt atttgacgaa
    13381 aaatattttg tgtctcgtaa tc
//
LOCUS       DBNFEG010000041        13254 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.041, whole genome shotgun sequence.
ACCESSION   DBNFEG010000041 DBNFEG010000000
VERSION     DBNFEG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13254
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.041"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(143..700)
                     /locus_tag="AC1PNG_000829"
     CDS             complement(143..700)
                     /locus_tag="AC1PNG_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIP6701343.1"
                     /translation="MTQAFFPIHTLETVSPELRENLATVKKNNGGYIPNLIGLLANSP
                     TALETYQTVSGINRRSSLNPTEREVVQITAAVANGCGFCVAGHTAISIKQVKMPDVIL
                     QALRQGTPIETDAKLDALARFTLAVIHEKGKVEQPLLEEFFQEGYTAENALDVVLGVS
                     LATLCNYANNLINTPINPELQAYAL"
     gene            complement(866..1561)
                     /locus_tag="AC1PNG_000830"
     CDS             complement(866..1561)
                     /locus_tag="AC1PNG_000830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIP6701344.1"
                     /translation="MKALIFDVDDTLYDQIQPFERALERHIEVAREQIEPLYLSFRRY
                     ADEVFEATAIGKMSLKDSHIYRMKHALADFGYQVSDATALAIQIDYDYFQGQIELSPV
                     FPEIFSWCQAQGIAMGIITNGPYRHQLRKIRTMGLVNWFELEHVLISGQVGITKPNPA
                     IFQLMEERLGMSGEDICYLGDSFENDVVGAKAANWKAIWFNHRKRVEPTAPYQADKVV
                     TDWDELVEVIQSF"
     gene            complement(1615..3354)
                     /locus_tag="AC1PNG_000831"
     CDS             complement(1615..3354)
                     /locus_tag="AC1PNG_000831"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6701345.1"
                     /translation="MTILGFLLKQIRRVKWLFIVAVGFYLLASTMVRLAPLLIQQAID
                     GPITDLSKGLPFDEAVFLNQSAQYMGMIILGAIGFYLSMRLLMHCANRIAENLRNQAY
                     DVMQRLPISYFDDKPAGKIATRIVNDTETLRTQFYGTLVNAFNNIVRLLFTYGVLFYM
                     NRSLGWLMLLLIPLYIGIQFAYKKMTDKPMKDFYDARSDVNTQVNETMNGASLIQLFG
                     QEERIMKEFEVTADKMRRADNKIIWAQSLATWNLSGFLQNLVIAAILTVVGYQFLAGQ
                     YGVTAGKLFVYVNYIEGVFIALGALVQQFPNMLRLFETGKRLMALLEEEVEDDCDRIL
                     EVEQGQVVFEHVNFSYEENRPVLKDITIRAEKGETVALVGHTGSGKSSIMNLLYRFYD
                     PQEGRVLIDGKNIRDYSRESLRSHMGIVLQDPYLFTGTIASNVSMNEEEADRTRIMQA
                     LEKVGAGPMLSRLEKGIDEPVVEKGAAFSSGERQLIAFARTLYSDPKILILDEATSHI
                     DTETEEIIQHAMEVVKEGRTTFIIAHRLSTIQNADQILVLDQGRIIEHGKHEELVARG
                     GVYAQMHEIQAKV"
     gene            complement(3354..5081)
                     /locus_tag="AC1PNG_000832"
     CDS             complement(3354..5081)
                     /locus_tag="AC1PNG_000832"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6701346.1"
                     /translation="MFRLIFDYIKRHKWLYLLVAVTLIIYDATLLLPTQIIQRMVDIL
                     TKNELTQAILVQEMTLLLLVTVLNYATAFIWHLKLFQASVNFKFDMQQRAFKKLVTMR
                     TPFYEKFRSGDVMTRFSTDVDGLMEMVGYGLMIVVYAGGMLAFIIPTMFFIDWKISLV
                     ALLPMLFMTLCIFFIGRKQDKAIDANREAVAQLNNEVLEVIEGIRVTRAYSKKATQKA
                     QFQARTKQLAQGGDRITSLQSLYNPLATVCLGLSTIFVLLMGAQAVKAGQLTLGQVIA
                     LQLYVGSLLEPFWTLADFILVYQTGKTSFEKLQELIETGDDLEADGSKEIAELSSISF
                     KNYSFSYPQAERASLQDINWTLKAGQTVGIVGKTGSGKTTLVRQFLRQYPIGQGNFFI
                     NHQSVLDFKRSSIEEKIGYVPQEHILFSRSVGENIALGKVASSSEEIEQAIATAAFSQ
                     DLKRMTDGLDTMIGERGVSISGGQKQRISIARAFLREPDLLILDDSLSAVDARTERQI
                     IQNIQKERAGKTNVIVTHRLSAVNHADWVLVLDEGRIVEEGRPADLLAQRGWYYEQYQ
                     RQQSQEGGE"
     gene            complement(5650..5722)
                     /locus_tag="AC1PNG_000833"
     tRNA            complement(5650..5722)
                     /locus_tag="AC1PNG_000833"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5687..5689),aa:Thr,seq:ggt)
     gene            5879..6349
                     /locus_tag="AC1PNG_000834"
     CDS             5879..6349
                     /locus_tag="AC1PNG_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIP6701347.1"
                     /translation="MKLQNLQVGQSYLVKDCLTQDDIRKHLAHLGLKVGEEIRIISKT
                     KTNAIFQVKASRLALDREIIESLVLTEKSATEIINLSEAPIGSSAKVMDIYATGALRR
                     RLMDMGLTKNTQLFLKKVAPLGDPIEITLRGYELTLRKSEAQMIGVQITSEVRK"
     gene            6346..8490
                     /gene="feoB"
                     /locus_tag="AC1PNG_000835"
     CDS             6346..8490
                     /gene="feoB"
                     /locus_tag="AC1PNG_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIP6701348.1"
                     /translation="MTKHIALAGNPNSGKTTLFNLLTGTNQRVGNWPGVTVERKSGTI
                     KKRKHLLIQDLPGIYSLSPYTPEERVARDYLIADQPAAILNVLDATNLERNLYLTLQL
                     LEMGLPIAIALNMTDALKSQGRSINSDQLSYQLGVPVQPISALKKLGISQLLHEVEKI
                     TNQQAEPIYPQYDKQFEAGLAQVIDLLSDSIPNHQKRFYAIKLLEQDQVILDQLQLNA
                     QDMLDLTEIIAILEKIYADDMEAIIVNQRYQFIEKITELVTKDSDKAFNLSDNIDRLV
                     TNRFLALPIFAAVMWLTYFLSIQTVGTMGTDWVNDVLFGELVPGLIQANLDRFEIAGW
                     LQSLVLDGIIAGCGAILGFVPQIFVLFVCLGILEDIGYMSRVAFVMDRIFRRFGLSGK
                     SFIPMLISTGCGVPGVMASRTIENEQDRKITIMTATFMPCSAKLPIISLVAGAFFPDN
                     PWIAPSAYFVGMAAIVLSGMALKKTKQLGGVASPFIMELPSYHLPKLSTVLRYAFDKA
                     LSFIKRAGTIIFVTNIIIWFSSSYNWSLQMVETDESILASIGHSFSLLFAPLGFENWR
                     ATVAAITGLLAKETVIATFGILYKLGETTEENPELWGLLQQDYTALSAYSFLVFNLLC
                     APCFAAIGAIHREMGEAKWTWIAIGFQTGLAYASSLVIYQIGLVLIHGQLPTVWTFIA
                     IFLIITAIYSLVKKPTNTLPIVTLKTLEKGEY"
     gene            8490..8642
                     /locus_tag="AC1PNG_000836"
     CDS             8490..8642
                     /locus_tag="AC1PNG_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HIP6701349.1"
                     /translation="MPTLILFLIIVILFSLAIRSLIKGKGSCGDCSCDCTIKQEMNES
                     SHHRHP"
     gene            complement(8695..9624)
                     /gene="ftsX"
                     /locus_tag="AC1PNG_000837"
     CDS             complement(8695..9624)
                     /gene="ftsX"
                     /locus_tag="AC1PNG_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIP6701350.1"
                     /translation="MSNRFFRHFIESLKSLKRNGWMTIAAISSVAITLTLVGLFASVI
                     LNTAKLASDLEQNVRINVYLRANSTDQAETIVNEAGKTVANPDYKKVYNQITALENVQ
                     SVTYSSKDEQLQKLTATLGDTWNLFQGDANPLYDAYIIDTTEPQYVKTVAAEIAKIDG
                     VTEVRDGEVETERIFKLANLVRTWGLAATGLLLFTAVFLISNTIRITIISRSREIQIM
                     RLVGAKNSYIRGPFLWEGAWVGLLGAILPSALVYSFYKMIYTSVNASLASQDLSLISM
                     DVFVPGMIGALFVIGIIIGSLGSVISMNRYLKI"
     gene            complement(9617..10309)
                     /gene="ftsE"
                     /locus_tag="AC1PNG_000838"
     CDS             complement(9617..10309)
                     /gene="ftsE"
                     /locus_tag="AC1PNG_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIP6701351.1"
                     /translation="MGIIEMKDVSKKYGNGTTALRGVSVNVEAGEFAYIVGPSGAGKS
                     TFIKLLYREEKLDKGSLKVGKFDLAKIKKRDVPLLRRSVGVVFQDYKLLPKKTVFENI
                     AYAMEVIGEKPRNIKKRVMEVLDLVGLKHKIRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPENSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIEDGRVV
                     RDEAEGEYGYDE"
     gene            complement(10452..11547)
                     /gene="prfB"
                     /locus_tag="AC1PNG_000839"
     CDS             complement(join(10452..11474,11476..11547))
                     /gene="prfB"
                     /locus_tag="AC1PNG_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775232.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIP6701352.1"
                     /translation="MDTAEIRQKIEELGKKLTSFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNLAAQKTSQELNELKNTYGNFHQMLDLYDESEILLDFLAEDESVRDELVEKLVE
                     LDKTMTAYEMTLLLSDPYDHNNAILEIHPGSGGTEAQDWGEMLLRMYQRYGNAKGFIV
                     ETLDYQAGDEAGIKSVTLSFTGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEIEIRDDEVKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVTQSTVDRT
                     QYGNRDRAMKLLQAKLYQLEQEKKAAEVDSLKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTGYEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            complement(11582..12709)
                     /gene="queG"
                     /locus_tag="AC1PNG_000840"
     CDS             complement(11582..12709)
                     /gene="queG"
                     /locus_tag="AC1PNG_000840"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIP6701353.1"
                     /translation="MNIKEEIIKLSQEIGISKIGFTTADDFAYLEKSLRASQEEGRSS
                     GFEHKNIEERIRPKLSLESAKTIISIAVAYPRHLPVKPQKTQYKRGKITPSSWGLDYH
                     YILQDKMERLARGIERLTDGLEYKAMVDTGALVDTAVARRAGIGFIGKNGLVISKEFG
                     SYMFLGELVTNLDIEPDQPVDYDCGDCNRCVAACPTSCLLGDTTMNARRCLSFQTQDK
                     GMMDLEFRKKIKTVIYGCDICQICCPYNKGISSPPVVEIDPDLAEPELIPFLDLSNGQ
                     FKEKFGMIAGSWRGKNILQRNAIIALANSNDRSAIPKLLEIIDKKQNPVHMATAIWAL
                     GQLVKQPNDEMVSFIAGISSDNDDVIAEQAAFLNMVKDLQM"
     gene            complement(12750..13238)
                     /locus_tag="AC1PNG_000841"
     CDS             complement(12750..13238)
                     /locus_tag="AC1PNG_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIP6701354.1"
                     /translation="MQTKKMSMFLFFAYLLLLTWMIIFKMDLSIVYGRYGYASINLIP
                     FAGTAVYDGVLDFPEILFNIVSFIPFGIYMEMLFRKASWVTNLCLIMLVSLCFEVLQY
                     LLLLGVADITDLLANGLGGAIGINIMYVLTSIWREKAYVRMNIFCFVLTFFVILITYL
                     AM"
BASE COUNT         4028 a         3094 c         2350 g         3782 t
ORIGIN      
        1 aggcaagttg acaacggata attttgattt tcgaagagta tcaaatgtat attaggaaaa
       61 tcacacttgg ttaattcaaa aaaactagtt aggataatgt aaacgtccta actagtttgc
      121 gatacatatt ctgctggaac tcctacagag cataggcctg taattctgga ttgataggag
      181 tgttgatgag attgttggca taattacaaa gagttgcaag actgactcct aacaccacat
      241 ccaaggcatt ttcagccgtg tagccctctt ggaaaaattc ctctagcaag ggctgttcaa
      301 ccttgccttt ttcatggata acagccagag taaatcgagc gagagcatcc aattttgcat
      361 ccgtttcgat gggagttccc tggcgtagag cctgtagaat aacatcaggc atcttaacct
      421 gcttgataga aatagccgta tgacctgcca cgcaaaatcc acagccattg gcaacggcag
      481 ctgtaatttg taccacttca cgctccgtcg gattcagact actacggcga ttgataccac
      541 taacagtttg gtaggtttct agggctgtag gagaattggc taaaagaccg atgagattag
      601 gaatataacc gccattattt ttcttgacag tcgcaaggtt ttctcgtaat tctggactaa
      661 cagtctctag agtatggata ggaaagaaag cttgtgtcat aggtaactcc atttcaaaaa
      721 atttcataga gtaggaataa aaatctgctt ggattcacac ctatacttct atgcttgcat
      781 tttatactat cataaagaca ggcatcctgc taatatattt tacctatcac acctataaaa
      841 aattgataat cccttgtaga aaccactaga aagactggat aacttcgacc agttcatccc
      901 aatctgtcac cactttatct gcctgatacg gagctgttgg ctctactctc tttctatgat
      961 taaaccaaat tgccttccaa ttcgcagctt tcgctcctac cacatcattt tcaaacgaat
     1021 cccctagata acaaatatct tcgcctgaca tccccaaacg ttcttccatt agttgaaaaa
     1081 tagcaggatt tggctttgtg ataccaactt gtccagaaat taggacatgt tctagctcaa
     1141 accaattaac taaccccatc gtgcgtatct ttctcaactg atgtctataa ggtccattcg
     1201 taataatccc cattgcaatt ccttgcgcct gacaccatga aaatatttct ggaaacactg
     1261 gactgagctc tatctgccct tgaaaataat cataatctat ttgaatagct aaggctgttg
     1321 catcagacac ctgataccca aaatctgcca aagcatgttt catccgataa atatgactgt
     1381 ctttcagact catctttcca attgctgtcg cttcaaatac ttcatccgca tatctgcgaa
     1441 aggaaaggta gagtggttca atttgctccc ttgctacttc aatgtgtctt tccaaagcac
     1501 gttcaaacgg ttgaatctga tcataaagcg tatcatctac atcaaaaatt aaggctttca
     1561 tgagtcaatt atagcaaaaa attccagcca aagcttggaa tttttaactg cttattacac
     1621 ctttgcttga atctcatgca tttgagcata tactccacca cgtgcaacca attcttcatg
     1681 cttcccatgt tcaataattc ggccttgatc taagacaaga atctggtctg cattttgaat
     1741 ggtagaaagt cggtgggcaa tgataaaggt tgtccgacct tctttcacca cttccatagc
     1801 atgttggata atttcttccg tctccgtatc aatatgagaa gttgcttcat ccaatatcag
     1861 aattttagga tcagagtaga gtgtccgcgc aaaggcaatc aactgacgtt ccccacttga
     1921 aaaggccgcc cccttctcaa caaccggttc atcaatccct ttctctaagc gagatagcat
     1981 aggccctgca ccaacttttt ctaacgcctg catgattctt gttctatctg cttcctcttc
     2041 attcatggaa acattgctag caatcgtccc tgtaaagaga tagggatctt gcaaaacaat
     2101 ccccatgtga ctgcgcaggc tctcacgaga ataatcacgg atgtttttcc catcaatcag
     2161 tacacgtccc tcctgtggat catagaaacg atagaggagg ttcataatag aagacttacc
     2221 agacccagta tgcccaacca gagcaactgt ttctcctttt tctgcccgaa tggtaatatc
     2281 tttcaaaact ggtctgtttt cttcatagct aaagttcacg tgttcaaaca cgacttgccc
     2341 ttgttcaact tccaaaatac gatcacagtc gtcctccacc tcttcttcta gaagggccat
     2401 tagacgctta cctgtttcaa acaagcgaag catatttggg aactgttgaa ccagcgctcc
     2461 caaagcaatg aagactcctt cgatatagtt gacataaaca aagagtttcc cagctgtaac
     2521 tccatactgt cccgcaagaa actgatagcc gactaccgtt aaaatcgcag caatgaccaa
     2581 attttgaaga aaaccactca gattccacgt ggcaagagac tgggcccaga taatcttatt
     2641 atctgcccga cgcatcttgt cagcagtcac ttcaaattct ttcatgatgc gctcttcttg
     2701 tccaaaaagt tgaatcaaac ttgcaccatt catggtctca ttcacctgcg tattgacgtc
     2761 actccttgca tcataaaaat ccttcatcgg cttgtctgtc attttcttgt aagcaaattg
     2821 aataccgata tagagtggta tcaagagtaa catcagccaa ccaagactcc tattcatgta
     2881 aaataggaca ccgtatgtaa agagcaaacg aacaatattg ttaaaggcat taactaaggt
     2941 cccataaaat tgtgtccgca gagtttctgt atcattgaca attctagttg caatttttcc
     3001 agcgggctta tcatcaaaat aagaaatagg caaccgttgc ataacatcat aagcttgatt
     3061 acgtagattt tcagcaatac gattggcgca atgcatgagt agacgcatag ataagtagaa
     3121 gccaatagct cccagaataa tcattcccat gtattgagct gactggttta agaaaacagc
     3181 ttcatcaaaa ggcaaaccct tgctcaaatc cgtaatcggc ccatcaatcg cctgctgaat
     3241 cagtagagga gccaatcgaa ccatggtcga agccaataga tagaagccga cagctactat
     3301 aaatagccac ttaacacgac ggatttgttt taataaaaaa cctaaaatag tcattactct
     3361 cctccttcct gactttgttg tcgttggtat tgttcatagt accaacctct ttgggcaagc
     3421 aaatcagctg gacgcccttc ctcaacgata cgcccctcat ccaagactag tacccagtct
     3481 gcatggttga cagcagatag acgatgggtc acgatgacat tggttttgcc tgcacgttct
     3541 ttttgaatat tttggataat ctggcgttct gtcctagcat ccactgccga caaagaatca
     3601 tctagaatca gcaaatctgg ctctcgcagg aaagcacggg caatggaaat ccgttgtttc
     3661 tgaccgccag aaatagacac accacgctca ccaatcatgg tatcaagacc atcagtcatc
     3721 cgttttaaat cttgactaaa agctgctgtg gcaatggctt gttcaatctc ctcagagcta
     3781 ctagctactt tcccaagggc aatattttct ccaacagacc ttgaaaacaa aatgtgttct
     3841 tggggaacat agccaatttt ttcctctata ctagaacgtt taaagtctaa aacagattga
     3901 tgattgatga aaaaatttcc ctgaccaatc ggatactggc gtaagaattg gcgaaccaag
     3961 gtcgtttttc ccgaaccagt tttcccaacg atacctacag tttgccctgc tttcagtgtc
     4021 caattgatgt cctgtaggct ggctctttcc gcctgtggat aactaaagct ataatttttg
     4081 aaggaaatac tagacagctc tgcaatttcc ttagaaccat cagcctccaa atcatcaccc
     4141 gtctcaatca gctcttgcag tttttcgaaa gatgtcttac cagtctgata aacaaggatg
     4201 aaatctgcta gtgtccaaaa cggctccaaa agcgaaccga catacaattg aagggcaatt
     4261 acttgtccca aggtcagttg ccctgctttc acggcttgtg cccccatgag aagcacgaaa
     4321 atagtagaca atcccaaaca gactgtggct aatggattat acaatgattg caaggaagtg
     4381 atgcgatccc caccttgagc caattgcttg gtacgagctt gaaattgagc cttttgagtt
     4441 gcttttttac tataagcccg agtcactcga atgccttcga tgacctccaa aacttcatta
     4501 ttgagctggg ctacagcttc tctattggca tcaatcgcct tatcttgttt tctcccgata
     4561 aaaaatatac atagtgtcat gaacagcatc ggtagtaaag ctactagcga aattttccaa
     4621 tcaataaaga acatcgtggg aatgataaaa gccaacatac cgcctgcata gacgacaatc
     4681 ataagaccgt aacccaccat ctccatcagg ccatccacat ccgttgaaaa acgagtcatg
     4741 acatctcctg agcggaattt ttcataaaat ggagtccgca tggtcactaa ttttttaaag
     4801 gcacgttgct gcatatcaaa cttgaaattg acagaagcct gaaataattt cagatgccaa
     4861 atgaaggctg ttgcatagtt cagtactgtt accaataaca ataaggtcat ctcctgaacc
     4921 aatatggcct gtgttaattc attcttggtt agtatatcta ccatccgctg aatgatttgc
     4981 gttggtagca aaagtgtggc gtcataaata atcaaggtca cagcaaccaa tagatagagc
     5041 cacttatgac gcttgatata gtcaaaaatt aatcgaaaca tatatctcct ttacaaatct
     5101 ataacctatc atttgccgct ttattcaata agcaacaatt gatagacgtt atactcaatg
     5161 aaaatcaaaa gtagcctaga aaacgatgtc gaagatagaa ctggagttca tcaaggcaag
     5221 ttgacaacgg ataattttga ttttcgaaga gtattacctt tctaagcgca caaaaaagcc
     5281 tagggaagca tctcactcca cctaggcttg cgtaagtcca caaaaataga ccagctcttg
     5341 aacatagtcg acaattttta tcacaccata cccacagtgc ttgattgtct caatgtttca
     5401 aaatatagcc cacgaaaaac gacaaaagtt catcaatgag ctttgttttc ctgcttagaa
     5461 aggagtgtat gataaaaaat agcttgctga ataacaagtt gcttgttcat ttgttttact
     5521 cctttcatat ttctaagccc tattctacca taataaacat tcatgtcaag ggattttttg
     5581 acaataaggg cgaatttagg acaaaaataa gaaaaacctt gtcaaacaag gcctttcgat
     5641 agtattttat gctagttgcc gggattgaac cggcgacctc atccttacca tggatgcgct
     5701 ctaccgactg agctaaacca gcagtacttg actagtatat catatctcta aaaaaagtca
     5761 atggattttt tggaaatatt gagaaaaata aagagaaaat aagccattcc taaaaattgt
     5821 ctgacacttt taaggaattg tgctatacta ttgttataaa aatcacaaag gaaacgatat
     5881 gaaactgcaa aaccttcaag ttggacaatc ttatttagtc aaagattgcc taacgcaaga
     5941 tgatattcgt aaacacttag cgcacttggg tttaaaagtc ggagaagaga ttcgcatcat
     6001 ttcaaaaacc aagaccaatg caattttcca agtcaaggca agccgtctag ccctagacag
     6061 agaaattatt gaatctctgg ttttaacaga aaagtcagct acggaaataa tcaatctatc
     6121 tgaggcacct attggtagct cagccaaggt catggacatc tatgcaacag gtgcactccg
     6181 tcgtcgcctc atggatatgg gacttacaaa gaacacacag ctatttctca aaaaagtcgc
     6241 tcccctaggc gatccaatag aaattacctt gagaggatac gagctgacac tacgcaaatc
     6301 cgaagcgcaa atgattggtg ttcaaatcac aagcgaggtg aggaaatgac aaaacacatc
     6361 gcccttgcag gaaatccaaa tagcggaaaa acaactcttt tcaacctcct aacaggaacc
     6421 aaccaacgtg ttggtaactg gcccggagtc accgttgaaa gaaaatctgg aaccatcaaa
     6481 aagcgaaaac atttactaat tcaagattta ccaggtatct actcgctgtc tccctacaca
     6541 ccagaagaac gggttgccag agattatttg atagcggacc aaccagctgc tatcctcaac
     6601 gtccttgatg caacaaactt ggagcggaat ctctatctga ccttgcagtt attagaaatg
     6661 ggccttccta tagctatcgc cctcaatatg acagacgccc tgaaaagtca aggtcgctca
     6721 ataaacagcg accagctctc ctatcaactg ggagttcctg tacagcctat cagtgcacta
     6781 aaaaaactag gtatatccca gctcctccat gaagtagaaa aaattaccaa tcagcaagcc
     6841 gagccaatct atcctcagta tgataagcaa tttgaagccg gtcttgccca agttattgac
     6901 ctattatcgg attccatccc aaatcatcaa aaacgcttct acgccattaa gctactcgaa
     6961 caagatcaag tcattctaga ccaattgcaa ctaaatgcac aagacatgct tgatttgacc
     7021 gaaatcattg ccattttgga aaaaatttac gcagacgata tggaagccat tatcgtcaat
     7081 caacgctacc aatttatcga gaaaatcaca gaactggtca caaaagacag cgataaggct
     7141 tttaacctgt ctgataacat agaccgactt gttaccaatc gttttcttgc cctccctatc
     7201 tttgcggcag tcatgtggct gacctacttc ctgtctattc aaacggtagg tacaatgggg
     7261 actgactggg tgaatgatgt cctcttcgga gagcttgttc caggccttat tcaggcaaat
     7321 ctagatcgat ttgaaatcgc tggctggcta caatctctgg tccttgatgg cattattgca
     7381 ggatgcggag ccattctagg ttttgtacca caaattttcg tcctcttcgt ctgcttggga
     7441 atcttggaag acatcggcta tatgagccga gtcgcctttg tcatggatag gatttttaga
     7501 cgttttggtc tatcaggcaa atcctttatt cccatgttga tttccacagg ctgtggcgtt
     7561 cctggagtca tggcaagccg gactattgaa aatgaacagg accgaaaaat caccattatg
     7621 acagcaacct tcatgccctg ttctgccaaa cttcctatca tttcactggt agccggtgct
     7681 ttctttcctg acaatccttg gattgctcca agcgcctatt ttgtcggtat ggctgccatc
     7741 gtcctatccg gaatggcact aaaaaagacc aagcaacttg gtggagtcgc aagccccttt
     7801 atcatggaat taccatctta tcacttacca aaactcagta cggttctccg gtatgcattc
     7861 gataaggcac ttagttttat caaacgagct ggtaccatta tatttgtgac caacatcatt
     7921 atttggttca gcagttccta taattggtct ttacaaatgg tagaaacaga tgagagtatc
     7981 ttagcctcca ttggacacag tttttctctc ctctttgccc cacttggttt cgaaaattgg
     8041 cgagcaactg ttgcagccat tacaggtctc ttagccaagg aaaccgtcat tgccaccttt
     8101 ggtatcctct ataaattagg agaaacaacc gaagaaaatc ccgaactatg ggggctttta
     8161 caacaagact atacagcgtt atcagcctac tccttcctag tcttcaatct cctctgtgcc
     8221 ccttgttttg cggctattgg tgccattcac cgtgaaatgg gagaagccaa gtggacatgg
     8281 attgctatcg gtttccaaac gggtttagcc tatgcaagta gtttggttat ctatcagatt
     8341 ggtcttgtac ttatccacgg acaattacca accgtctgga catttatcgc tatctttcta
     8401 attatcacag ccatctacag cctagtgaaa aaaccaacca acactcttcc aatcgtcacg
     8461 ctaaaaacat tagaaaaagg agaatattaa tgcccacact tatcctcttt ctcatcatcg
     8521 tcatcttatt ttctcttgcg attcgcagtc tcatcaaagg aaaaggatcc tgcggagatt
     8581 gttcctgcga ctgtactatt aagcaagaga tgaacgaatc ttctcaccat agacatccat
     8641 aacaaaaagg tctgcctcat tattgtgcag acctttttac atgtagcttg tctattaaat
     8701 ttttaagtag cggttcatgg aaatgactga tcccaaggaa ccaataataa taccaattac
     8761 aaaaagtgcg ccaatcattc ctggaacaaa gacatccata ctaatcagag acaaatcttg
     8821 actagcaaga ctagcattta ccgaagtata aatcatctta taaaaagaat agactaaagc
     8881 agaaggcaaa atagctccga ggagacctac ccaggcacct tcccagagga atggtccacg
     8941 gatataactg tttttagcac caaccagacg cataatctga atttcacggc tacgagaaat
     9001 aatggtaatt cgaatagtat tggaaatcaa gaaaacagca gtaaagagca ataagccagt
     9061 tgctgccaat ccccatgtac gaactagatt agctaattta aaaatacgtt ccgtttcaac
     9121 ttccccatcc cgtacctctg taacaccatc aattttagca atctctgctg caacagtttt
     9181 tacatattgc ggttcagtcg tatcaatgat ataagcatcg taaagtggat tggcatctcc
     9241 ttggaatagg ttccaagtat ctcccaaggt agctgttaat ttctgtaact gttcatcctt
     9301 acttgaataa gtgacagatt gtacattttc taaggctgta atctgattat acaccttttt
     9361 ataatctgga ttagctaccg tctttcctgc ttcgttcaca atcgtctcag cttgatcggt
     9421 tgagttcgct ctcaaataca cattgatgcg tacattctgt tccaaatccg aagcgagctt
     9481 tgctgtgttc aaaataactg aagcaaatag accaaccaag gtaagggtaa tggctacaga
     9541 tgaaatggct gcaatcgtca tccaaccatt tcgcttcaaa ctcttcaatg actcaataaa
     9601 gtgtctaaaa aatcgattac tcatcgtatc catattctcc ttccgcttca tcacgtacta
     9661 cacgaccatc ttcaatcgca ataacacgat gacgaagtgt atttacaatc tgactattgt
     9721 gtgtcgccat caaaatcgtt gtcccctgta agttaattcg ctccaataga ttcatgattt
     9781 cccaagagtt ttcagggtcc aagttaccag ttggttcatc agcaatcaat actttaggat
     9841 tatttacaat ggcacgggca atagcaatcc gctgttgctc accacctgaa agttcatttg
     9901 ggaaagaacg aattttgtgc ttcaaaccaa ccaaatctaa aacttccatt acacgtttct
     9961 tgatattccg tggtttctca ccgataactt ccatcgcata ggcaatattt tcaaagacag
    10021 tctttttagg taagagtttg taatcttgga agaccactcc tacactacga cgtagaagag
    10081 gaacatctct ctttttaatt tttgcaaggt caaacttacc aacctttaga ctccccttat
    10141 cgagcttttc ctcccgatac aagagtttga taaaggttga cttacctgca ccagaaggac
    10201 ctacaatgta ggcaaattct cccgcttcaa cattgactga tactccgcga agggcagtcg
    10261 ttccgtttcc atatttcttg gaaacgtctt tcatttctat tattcccata ggatttcctg
    10321 tcttagacag tcctttcttt agatttgaga acgttttttt caaaatacag tccgtattct
    10381 ctaaaaaaac ttccttgatg agcgaaaaac tatctcttat ttacaagcac ttcacttgta
    10441 aatacaggtt cttagctaat tcgccatttt aaataagcat ctataaagcc gtccaagtct
    10501 ccgtccataa ccttatcgac ttgagccacc tcataaccag tacggtggtc ttttaccatt
    10561 gtataaggtg taaagacata agaacgaatc tgacttcccc aagtgatctc tttcttgtca
    10621 cccttaagtg agtctacttc tgccgctttc ttctcctgct ccaattgata caatttagcc
    10681 tgcaagagtt tcattgcacg gtctcggttt ccatactgag tacggtcaac tgtggactga
    10741 gtcacaatcc ctgtcggaat gtgcgtcaat cgaacacccg ttgaaacctt attgacgttc
    10801 tgtccaccag cacctcctga ccggaaggta tccatcttca cttcatcatc tctgatttca
    10861 atttcaatgg tatcatccaa ctcaggcatt acctccacag atgtaaagga agtatgacga
    10921 cgcttagctg agtcaaaagg tgagatgcgc accaaacgat gaacacccat ttctgactta
    10981 agcaagccat aggcgtttgg accagtaaag cttagagtca cagacttaat acctgcctca
    11041 tcaccagctt gataatccaa tgtttctact ataaaacctt tggcatttcc ataacgctga
    11101 tacatccgca ggagcatttc tccccagtct tgtgcctcgg taccacccga acctggatgg
    11161 atttccaaga tagcgttatt gtgatcatag gggtctgata agagcaaggt catttcgtaa
    11221 gcagtcatgg ttttgtccaa ttccactaac ttttcaacca actcatcccg cacagactca
    11281 tcttctgcta gaaaatctag caaaatttca gattcatcat ataaatccaa catctgatgg
    11341 aaatttccat aagtattttt taattcattc aactcctgag acgtcttctg ggctgccaaa
    11401 ttatcattcc agaaatctgg ctccgtcatc ttgttctcaa gaatggcaat ctcttcttcc
    11461 agaccttcta agtcaaagag accccctaaa agaagttaat tttttgccaa gttcttcaat
    11521 cttttggcga atttctgctg tatccatata cacctcattt ttattcataa tctttattat
    11581 atcacatttg caagtccttg accatgttga gaaaggccgc ttgctctgct atgacatcat
    11641 cattgtctga tgaaatacct gcgataaagg aaaccatctc atcattgggc tgtttgacca
    11701 attgccccaa ggcccagata gccgttgcca tgtggaccgg attttgtttt ttatcgatga
    11761 tttctaggag tttgggaatg gcagagcgat cattactatt agccaaggct atgatggcat
    11821 tacgctggag aatatttttc ccccgccaag aacctgcaat cataccaaat ttttccttga
    11881 attgtccatt tgacaagtcc aagaatggaa tcagctcagg ctcagccaaa tcagggtcaa
    11941 tctccaccac aggtggacta gaaattccct tgttgtaagg acaacaaatc tggcaaatat
    12001 cacaaccata gatgaccgtc ttgattttct tacgaaactc caagtccatc atacccttat
    12061 cctgggtctg aaaggacaga cagcgacggg cattcatggt cgtgtcacct aatagacaag
    12121 acgtcggaca ggctgcaaca cagcgattgc aatcaccaca atcataatct actggttgat
    12181 ctggttcgat gtccaggttg gtcactaatt ctcctagaaa catataggaa ccaaattcct
    12241 tagaaatgac tagcccattc ttaccgataa agccaattcc tgctcttctg gcaacagctg
    12301 tatccaccaa agctcctgta tctaccatag ccttgtattc caaaccatcc gtcagtctct
    12361 caattccccg tgccaaacgc tccatcttat cttgcaagat atagtgataa tcaagccccc
    12421 atgaactagg ggtaatcttc ccacgtttgt attgggtctt ctgcggttta acaggcaaat
    12481 gtcgaggata agctaccgcg atagaaatga tggttttggc agattctaac gacagtttgg
    12541 gacggatacg ttcttcaata ttcttgtgtt caaatcctga cgaacgccct tcttcctgac
    12601 tagcacgaag tgatttctcc aaataagcaa aatcatcagc cgttgtaaaa ccaatcttcg
    12661 agattccgat ttcctgagat aatttaataa tctcttcctt aatattcatt atattcattt
    12721 tagcaaactt tgaatatatt tagcaagacc tacatggcta aataagttat taatataaca
    12781 aaaaacgtta gcacaaaaca aaaaatattc atacgaacat aggccttctc ccgccaaata
    12841 ctggtcaaga catacatgat attaataccg attgctcccc ctaagccgtt agccagtaaa
    12901 tcagttatat ctgcaacccc aagtaaaaga agatattgca gaacctcaaa gcagagactc
    12961 actaacataa tcaaacatag attagtcacc cacgatgcct tgcgaaatag catttccata
    13021 taaataccaa agggaataaa acttacaata ttaaatagaa tttctggaaa atccagcact
    13081 ccatcataga ccgctgttcc tgcaaacgga attaagttta tactagcata accatagcgt
    13141 ccatatacga tgctcaggtc catcttaaag ataatcatcc aagtaagtaa aaggagatag
    13201 gcaaaaaata aaaacatact cattttcttt gtctgcatag aaattctcct ctca
//
LOCUS       DBNFEG010000042        13010 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.042, whole genome shotgun sequence.
ACCESSION   DBNFEG010000042 DBNFEG010000000
VERSION     DBNFEG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13010
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.042"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(48..383)
                     /locus_tag="AC1PNG_000842"
     CDS             complement(48..383)
                     /locus_tag="AC1PNG_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701355.1"
                     /translation="MKVLVMSYMVIYLLVTLGAALFSYLKTKKMNTLRLILTILSMIL
                     LTSTLYFYSQSYHDLQMVGFALGFTFISTLFLYNGTKEGSNFTTVMLFSIGRFILHIQ
                     FLILLYLFR"
     gene            complement(384..2252)
                     /locus_tag="AC1PNG_000843"
     CDS             complement(384..2252)
                     /locus_tag="AC1PNG_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIP6701356.1"
                     /translation="MSDFIVEHLTKSVGDKTVFADLSFIIHQGDRIGIIGVNGTGKTT
                     LLDVLSGRIGFDGDVSPFRTKNAYKIAYLTQEPDFDESKTVLDTVLSSDLRETQLIRE
                     YELLLSHYDEASQARLEKVMAEMDSLNAWEIESQVKTVLSKLGLTDLNKTVVELSGGL
                     RRRVQLAQVLLGNADLLLLDEPTNHLDIDTIEWLTTFLKNTKKSVLFITHDRYFLDNV
                     ATRIFELDRASLTEYQGNYQDYVRLKAEQDERDAALRHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKGDLANKIDDSELEINFETSRIGKKVINFENVSFSYPDKPILAD
                     FNLLIQNKDRIGIVGDNGKGKSTLLNLIAGDLQADSGKVDIGETIRIGYFSQTIKGLD
                     ESKRVINFLQEVAEEAKTTSGMVSIAELLEQFLFPRNTHGTLIEKLSGGEKKRLYLLK
                     ILLQRPNVLLLDEPTNDLDIATLTVLEHFLQSFTGPVITVSHDRYFLDKVANKILAFE
                     DSGIETFFGNYTDYLDEKAFLASSSAISLEKPKEKTEKIKENKKRMSYFEKQEWATIE
                     EDIAGLEERIAAIEAEMLTCGSDFTKLSDLQKELDEKNDLLLEKYERYEYLSELEG"
     gene            complement(2245..3456)
                     /locus_tag="AC1PNG_000844"
     CDS             complement(2245..3456)
                     /locus_tag="AC1PNG_000844"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIP6701357.1"
                     /translation="MKLNNLPSEFQEALPILEKIKAAGYEAYFVGGSVRDAILGRPIH
                     DVDIATSSYPQETKQIFSRTIDVGIEHGTVLVLEGKKEYEITTFRTEEEYVDFRRPSQ
                     VSFVRSLEEDLKRRDFTVNAFALDEEAQIIDLFEGMTDLENRTLRAVGIPAERFNEDA
                     LRIMRGFRFAATLDFEIEPTTFEAMVKTAPLLEKISVERSFIEFDKLLMADFWRKGLR
                     AMIDSKAYNFLPDLAGKSDELETMLTSLTEEFRFSTSEQAWAMLFVCLGIDSIKSFLK
                     KWKTSNDFQRSVVKLVEIYQLRQAGPVTKQICFKYGKEFLYLVEELRQAQGLVTDFVA
                     IDKIDQALTIHDKHEIVVNGGHLMKAFDLKPGPVLGELLKEVEFQIVEGQLENEEQAI
                     MTFVKGILENE"
     gene            complement(3453..4220)
                     /gene="dapB"
                     /locus_tag="AC1PNG_000845"
     CDS             complement(3453..4220)
                     /gene="dapB"
                     /locus_tag="AC1PNG_000845"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIP6701358.1"
                     /translation="MTIKVIIAGFKGKMGSTAVEMVKGDAALSLAALVDPFATETEVD
                     GVPVFKTKEEVASLEADVWVDFTTPKFAYENTRFALENGFAPVVGTTGFTPEEIEELT
                     ALSAEKGLGGLIAPNFAIGAILLMQFAAQAAKYFPNLEIIELHHDKKKDAPSGTAVKT
                     AELISQVRQSQTQGAADEEELIAGARGAAFDGFRIHSVRLPGLVAHQEVIFGAQGEGL
                     TIRHDSYDRISFMGGVNLGIKEVVKRSQLVYGLEHLL"
     gene            complement(4229..5080)
                     /locus_tag="AC1PNG_000846"
     CDS             complement(4229..5080)
                     /locus_tag="AC1PNG_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIP6701359.1"
                     /translation="MKLAVITDSSAILNVQSIERDDLFVLSIPVSIDGENYIEGQNLT
                     VEEFYQKMASSKELPKTSQPSLMELEEILAKLTEKGYTHALGLFLSSGISGFYQNIQY
                     LADEFEGLTVAFPDTKITSAPLGMMVENVLKWADAGLSFEEITGKLDQEIGKTTAFIM
                     VDDLNHLVKGGRLSNGAALLGNLLSIKPILYFTGEGKIEVYEKVRTEKKAIKRLLEIL
                     QEQTTEGQYQIAIIHANAPQKAENFKQQLEEAGVGSDLPIVSFGSVIGTHLGEGAVAF
                     GISPIIE"
     gene            complement(5082..5456)
                     /locus_tag="AC1PNG_000847"
     CDS             complement(5082..5456)
                     /locus_tag="AC1PNG_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HIP6701360.1"
                     /translation="MEIIRDKEFVNQYHFDARNHAWEKENGIPETKLKVDFQLIEQNR
                     AENRTSMITILRFMIVLDHFVISGAMSQAVHLPNRLVEEPTEFTDEEKRVLVEPLLDI
                     LKRMTYEVTEIAFDAPGVNLEF"
     gene            complement(5552..7507)
                     /locus_tag="AC1PNG_000848"
     CDS             complement(5552..7507)
                     /locus_tag="AC1PNG_000848"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HIP6701361.1"
                     /translation="MKIQDVLRKDVMLLDLQATSKEAVIDEMIASLVDKGYVTDFDVF
                     KTGIMNREAQTTTGLGDGIAMPHAKNAAVKEATVLFAKSNNGVDYASLDGQPTDLFFM
                     IAAPEGANDTHLAALAELSKYLMKAGFADRLRAATNPEEVIAVFDTAEAADKAVEEVV
                     AAPSGDRPFIVAVTACTTGIAHTYMAEEALKKQAAEMGVGIKVETNGASGVGNKLTAE
                     DIKNAAGVIIAADKAVDMPRFNGKPLVSRPVAAGIKQPEELINIILNGKAAAYTASEG
                     AATVESSEKLSLGKAFYKHLMSGVSQMLPFVIGGGILIALAFLFDGALGVPQDSLGSL
                     GSYHEIAAMFMKIGGAAFGFMLPLLAGYIAYSIAEKPGLVAGFVAGAIASSGLAFGKI
                     PYAAGGEETLALAGVSSGFLGALVGGFLAGGVVLVLRNALRNLPKSLQGLNAILLLPL
                     LGTAITGFLMFFVNIPMAAINTGMNNFLAGLEGSSAILLGLVLGGMMAVDMGGPVNKA
                     AYVFGTGTLAATVADGGSVAMAAVMAGGMVPPLAVFVATLLFKDKFTQEERNSGLTNI
                     VMGLSFITEGAIPFGAADPARAIPSFIAGSALAGALVGLSGIQLMAPHGGIFVIALTS
                     NPLLYIAYVLIGAVVSGVLYGALRKAK"
     gene            complement(7504..8415)
                     /gene="pfkB"
                     /locus_tag="AC1PNG_000849"
     CDS             complement(7504..8415)
                     /gene="pfkB"
                     /locus_tag="AC1PNG_000849"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HIP6701362.1"
                     /translation="MIYTVTLNPAIDYIVRLEHVETGSVNRMESDDKYAGGKGINVSR
                     VLKRLGYPNTATGFLGGFTGQFIKDGLIAEGIDTDFVQVDQDTRINVKIKADQETEIN
                     GLGPIVTDTQLAELEMVLAGLTKEDTVVFAGSAPASLGNEVYNRLIPVAKKAGAQVVC
                     DFEGQTLLDSLAHQPLLVKPNNHELEAIFNVELNDIADIETYAKKILEMGAQNVIISM
                     AGDGALLVTPAATYFAKPIKGTVKNSVGAGDSMVAGFTGEYVRSQDPIEALKWGVACG
                     TATAFSDDLASIEFIKETYEKVEVETL"
     gene            complement(8412..9161)
                     /locus_tag="AC1PNG_000850"
     CDS             complement(8412..9161)
                     /locus_tag="AC1PNG_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIP6701363.1"
                     /translation="MNILKSERKQIILERIHSQQFVRLEELIEVLDTSESTVRRDLDE
                     LENEGKLRRVHGGAEAPRNLQLEESILEKSVKNVQEKREIAERAAQQIVNGDVIFLDA
                     GTTTGVLIDYLQQENLTVVTNSIHHAVRLVERKIKTIIIGGFVKQSTDASVGAVALEQ
                     IRQLNFDKAFLGMNGIDKNYFTTPDIEEATIKRTIIDNAKESYVLADASKIGQFSFVK
                     VAAIEKSNIICQSSESSLLDIIKEKTRVIEV"
     gene            complement(9265..10320)
                     /locus_tag="AC1PNG_000851"
     CDS             complement(9265..10320)
                     /locus_tag="AC1PNG_000851"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIP6701364.1"
                     /translation="MKRKVFEHYQPYKIKQKARFDLGNNENRIIDWSFLPQETLENLD
                     VNQLSFYGDNTYSELIEKYAAYLGVNPKQVTVGVGSDHLIHMIVSTFMEKDDTFLTVN
                     PDFFMYETYNHMHGSRFETIDLEAKNDTLVLPVEKLLARAEEVKAKIIMLSNPNNPSS
                     VAYSLEDLEKLAISFKGLLVVDEAYVEFANVESFISRLQQFDNVLVLRTLSKAFGLAG
                     LRVGFAVGSEELIYELDKVIPPFSLSNLTAKMAAVALNYTDKVLETVGVIQNLREEMI
                     LFLQGLDNCQVLPSQASFVTFKAPWAEEFYQQALAQDWNFKYYPTGKLEGHIRLSIGR
                     PEEMDMMKKMIEKLVEA"
     gene            complement(10351..11076)
                     /gene="trmD"
                     /locus_tag="AC1PNG_000852"
     CDS             complement(10351..11076)
                     /gene="trmD"
                     /locus_tag="AC1PNG_000852"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIP6701365.1"
                     /translation="MRIDILTLFPEMFAPLEHSIVGKARDKGLLEINYHNFREKAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDTMDSIEQAKPRVILLDPAGRTFNQAYAEELAQEEQ
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKEVSH
                     TDDSFSSGLLEYPQYTRPYEYRGMVVPDVLMSGHHENIRKWRLEESLRKTYQRRPDLL
                     ENYNFTAEELTIFEKIKAEDTVD"
     gene            complement(11066..11584)
                     /gene="rimM"
                     /locus_tag="AC1PNG_000853"
     CDS             complement(11066..11584)
                     /gene="rimM"
                     /locus_tag="AC1PNG_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIP6701366.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAKLALFDDK
                     DQFVMEVEIANHRKAKNFDIIKFKGMYHINDIEKYKGFSLKIAEENLTDLEDGEFYYH
                     EIIGLDVYENDILIGQIKEILQPGANDVWVVKRKGKKDLLLPYIPPVVLNIDIPNNRV
                     DVELLEGLDDEN"
     gene            complement(11682..>13010)
                     /locus_tag="AC1PNG_000854"
     CDS             complement(11682..>13010)
                     /locus_tag="AC1PNG_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIP6701367.1"
                     /translation="KEKLWLFIKIFLPILIYQFANYSASFIDTMMTGQYSTMDLAGVS
                     MATSLWNPLFSFLTGIVSALVPIIAQYLGKGERDKIREEFHQFVYLAIGLTILLLCSV
                     LFIAVPALAQFGLDESIFQVGRQYLYFISIGILPLLLFSVCRSFFDALGLTQLSMYLM
                     LLLVPFNSLFNYLLIYGKMGLPALGGAGAGLGTALAYWAVLLVIVIVMCKNKTIASYQ
                     IWQWSPIDLSLLKEGLKIGLPIGLQVFAEVAIFAVVGLYMAKFSAQIIAAHQSAMNFA
                     TLLYAFPASVSSALAIVIAYEVGAKRPQDVKAYSRLGRLVALGFAVLTLTFLYFFRSK
                     VAYLYGNDAEFIRLTSHFLSFALMFQLADAFTAPIQGILRGYKDTTVPFILGLVAYWS
                     MTFPVAFLLERFFQMGPEAYWIGLISGIFVCGIALNLRLIIIAHSHLSVK"
BASE COUNT         3791 a         3017 c         2501 g         3701 t
ORIGIN      
        1 ttgcgtaaag cctttgcaaa aatttgtacg gataaaaatt aattgctcta tcgaaagaga
       61 tagagcaaaa ttaaaaattg aatatgtaag ataaaccttc caatggaaaa gagcataact
      121 gtcgtaaaat tactaccttc tttcgttcca ttatagagaa aaagagtaga aataaaggta
      181 aatcctagag caaaccccac catctgcaag tcgtgataag actgactata aaaatacaag
      241 gtgcttgtca gcaatatcat ggataggata gttaaaatca agcgcaatgt attcattttt
      301 ttggtcttta gatagctaaa caaagccgct cccaaggtca ccaagagata gatgaccata
      361 tatgacatga caagaacttt catctagccc tccagttcgc tcagatactc ataccgctca
      421 tatttttcca agagcagatc gtttttctca tccaattcct tctgcaaatc ggataatttt
      481 gtaaaatcac taccacaggt cagcatttcc gcctcgattg ccgcaatccg ctcctccaag
      541 ccagcaatat cctcttcgat ggttgcccat tcctgcttct caaagtagga catccgcttc
      601 ttgttctctt tgattttctc ggttttctcc tttggttttt ccaaagaaat ggcagagctg
      661 gaagccagaa aggccttctc atccagatag tctgtgtagt taccaaagaa ggtctctata
      721 ccgctatctt cgaaagccaa gattttattg gctaccttgt ctaggaaata acggtcgtga
      781 ctaacggtaa tgactggacc agtaaagctt tgcaagaaat gttccaagac tgtcagagtc
      841 gctatgtcca agtcgttggt cggctcatct agtaagagaa cattgggccg ttgcaagaga
      901 atcttcagca aataaagtcg cttcttctcc ccaccagaca atttctcaat cagggtacca
      961 tgggtattac gtgggaagag gaattgctct aagagttcgg caattgaaac cataccagaa
     1021 gtcgtcttag cttcttctgc cacttcctgc aagaaattga tgacccgctt gctttcgtcc
     1081 aagcccttga tggtctgaga aaaataaccg atacggatgg tttcaccaat atctacctta
     1141 ccgctgtctg cctgcaaatc acctgcgata agattgagta aggtagattt tccttttcca
     1201 ttatcaccga caattcctat gcggtccttg ttttgaatga gcaggttaaa gtctgctaaa
     1261 atgggcttat caggatagga aaagctgaca ttttcaaagt taatgacttt tttaccgata
     1321 cgagaggttt caaaattgat ttccaattcg ctatcgtcta tcttgttggc taaatcgcct
     1381 ttaaggtcat ggaaacgatt gatacgggcc tgttgcttgg tagcacgagc ttgaggttgt
     1441 ctgcgcatcc aagccaactc ttgtttgtag agctgctctt tcttatggcg aagggcggca
     1501 tctcgctcgt cctgttctgc ctttaagcga acatagtcct ggtaatttcc ctgatattcg
     1561 gttaagcttg cgcgatccaa ttcaaaaatc cgtgtcgcca cattgtccaa gaaatagcga
     1621 tcgtgggtaa taaagagaac agacttttta gtatttttca agaaagtcgt caaccactca
     1681 atggtatcaa tgtccaggtg gttagtcggt tcatccagca agagcaagtc agcattgcca
     1741 aggaggactt gggccaattg cactcgtcta cgtagaccac ctgataactc aacaacggtt
     1801 ttgttgaggt ctgtcagacc gagttttgac agaacagtct tgacctgact ttcaatctcc
     1861 caagcattga gagaatccat ctctgccatg actttctcca gtcttgcttg acttgcttca
     1921 tcgtaatgag ataagagcaa ctcgtattca cgaatcagct gggtttcgcg taggtcagat
     1981 gaaagaacgg tgtctaaaac tgtcttactt tcatcaaaat caggttcctg ggtcagatag
     2041 gcgattttat aggcattttt tgtacgaaaa ggtgatacat ctccgtcaaa accgatacga
     2101 cctgacagga catccaacaa agtcgtcttt cctgtaccat tgaccccaat aatcccgatt
     2161 ctgtcgcctt gatggatgat gaaagacaga tcagcaaaga ccgttttatc tccgacagat
     2221 ttagtcaggt gttcaacgat aaaatcactc attctctaag attcctttca caaatgtcat
     2281 aatcgcttgc tcttcgtttt ccagctgtcc ttccacaatc tggaattcca cctctttgag
     2341 tagttctccc aatactggac ctggtttgag gtcaaatgcc ttcatcagat gtcctccatt
     2401 gacaacaatt tcatgcttgt cgtggatagt caaggcttgg tcaatcttat cgattgctac
     2461 aaaatctgtg acaagacctt gtgcctgacg aagttcctct accaagtata agaattcttt
     2521 gccatacttg aaacagattt gtttagtgac aggtcctgct tggcggagct gataaatctc
     2581 tactaatttg accacgctac gttggaaatc attgctggtt ttccattttt tcaggaagga
     2641 tttgatgcta tcaataccta ggcagacaaa gagcatggcc caagcttgtt cagatgtcga
     2701 aaagcgaaac tcttctgtca gacttgttag catggtctct aattcatcac tttttccagc
     2761 taaatcaggc aagaaattgt aagccttgga atcaatcata gcgcgcaagc cttttcgcca
     2821 aaaatccgcc atcaagagtt tatcaaactc gataaagcta cgttccaccg agattttttc
     2881 taaaagcggt gcagttttaa ccatggcttc aaaggtagtc ggctcaattt caaagtccaa
     2941 ggtcgctgca aagcgaaatc cacgcatgat acggagagca tcttcgttga aacgctcagc
     3001 cgggatgcct acagcccgta gggtgcggtt ttccaagtca gtcatccctt caaaaaggtc
     3061 aatgatttga gcctcttcat caagggcaaa ggcattaact gtgaagtcgc gacgtttgag
     3121 atcctcttct aaggaacgca caaaagaaac ctgactcggc ctacggaagt cgacatactc
     3181 ctcttctgtc cgaaaggtgg taatttcata ctcctttttc ccctctaata ccaacaccgt
     3241 accgtgttca attccgacat caatagtccg tgaaaagatt tgtttcgttt cctgagggta
     3301 gctggatgtc gcaatatcga catcatgaat aggcctaccg agaatggcat cacgaacgga
     3361 accgccaacg aaataagcct cgtaaccagc tgctttaatt ttctctaaaa tcggcaaagc
     3421 ctcctgaaac tcagaaggca gattgtttaa tttcataata agtgttccaa accataaacg
     3481 agttgtgagc gtttgactac ctctttaatg ccgagattga caccgcccat aaaggaaata
     3541 cgatcgtaag agtcatgacg aatggtcaag ccttctccct gtgccccaaa aatcacttcc
     3601 tgatgggcaa caaggcctgg caagcgtact gagtggatac ggaagccatc aaatgctgca
     3661 ccgcgagcgc cagcaatcag ttcttcttca tccgctgcac cttgagtctg tgactgacgg
     3721 acttgtgaaa tgagttcggc agttttgaca gccgtaccac tcggtgcatc cttcttctta
     3781 tcgtggtgta attcaatgat ttcaagattt gggaaatact tggctgcctg tgctgcaaat
     3841 tgcatgagca agatagcgcc aatagcaaag ttaggagcaa tcaagccgcc caaacccttc
     3901 tcagcagata aggcggtcaa ttcctcgatt tcttcagggg taaatcctgt cgttccaacc
     3961 acaggtgcga aaccattttc caaggcaaaa cgggtgtttt cataggcaaa ttttggcgtt
     4021 gtaaaatcca cccagacatc agcttctagg ctggcaactt cttctttggt cttgaaaaca
     4081 ggaacaccat ctacttctgt ttctgtcgca aagggatcca ccaaggcagc caaactaagt
     4141 gctgcgtcac ctttgaccat ctcaacagcc gttgagccca tttttccttt gaatcctgcg
     4201 ataattacct taattgtcat gcaaactcct attcaataat gggagaaata ccaaaggcca
     4261 ctgccccttc tcccaagtgc gtaccgatga ccgaaccaaa tgacacgatt ggtaaatcgc
     4321 taccgacacc tgcttcttcc aactgctgtt taaagttttc agccttctga ggtgcgttgg
     4381 cgtgtatgat agcaatctga tactgtcctt cggtcgtttg ttcttggagg atttccaaga
     4441 ggcgtttaat ggcctttttc tccgtccgaa ctttttcata cacttcaatc ttaccctcgc
     4501 ctgtaaaata cagaatcggt ttgatgctca aaagattgcc aagcaaggct gcaccattgg
     4561 acaagcgacc accttttacc aaatgattga ggtcatcaac catgatgaag gctgtagtct
     4621 tcccaatttc ttggtccaat ttcccagtaa tctcttcaaa actaagtcct gcatcagccc
     4681 atttgagaac attttcgacc atcatgccta aaggagccga tgtaattttg gtatctggaa
     4741 aggcaacggt taatccttca aattcatcag ccaaatattg aatattttga taaaaacctg
     4801 aaatacctga tgacaagaat aagccaagtg cgtgtgtata gcctttctct gttaatttag
     4861 ccagaatttc ttctagttcc atcaaactcg gttgacttgt tttaggtaat tctttcgatg
     4921 aagccatttt ttgataaaac tcttcaaccg tcaaattttg cccttctata taattttccc
     4981 catcgatgct gactggaata cttaggacaa acaggtcatc ccgctcaata ctttgaacat
     5041 tcaaaatagc cgatgagtca gttataactg ctaacttcat attaaaactc caaattcact
     5101 cctggtgcat caaaggcgat ttcagtaacc tcataggtca ttcgttttaa aatgtccaaa
     5161 agaggctcta ccaaaacacg tttttcctca tcagtaaatt cagttggctc ttctaccaat
     5221 cgatttggta agtgaacggc ctgactcatg gcacctgaaa tcacaaaatg atctagtaca
     5281 atcataaagc gcaaaatggt aatcatcgat gttcgatttt ccgcacgatt ttgctcaatc
     5341 aattgaaagt caactttcaa ttttgtttcc ggaattccgt tctctttttc ccacgcgtga
     5401 ttgcgggcat caaaatgata ctgattgaca aattctttat cgcgaatgat ttccatttca
     5461 agactcctct aataacaata atactcacat tataccataa tcctcgtgaa atagctaaaa
     5521 aaccttgcca gaagcaaggt ttcaatcaaa attatttagc tttacgaagt gctccgtaaa
     5581 ggacacctga tacaaccgca ccgatcaaaa cataggcgat gtaaagcaat ggattagatg
     5641 tcaaagcgat aacgaagata ccaccgtgag gagccatcaa ttggatgcct gacaaaccaa
     5701 ccaaggcacc tgcaagggca gaaccagcga taaagcttgg gattgcacga gctgggtcag
     5761 cagcaccaaa tggaatcgca ccttcagtga tgaatgaaag acccataacg atgtttgtca
     5821 aacctgagtt acgctcttcc tgtgtgaatt tgtctttgaa aagaagggtt gcaacgaata
     5881 ctgccaacgg tggaaccata ccacctgcca ttaccgctgc catggcaaca gaaccaccat
     5941 ctgcaacagt tgctgcaagt gtacctgtac cgaagacata agctgccttg ttgactggac
     6001 cacccatatc gactgccatc attccaccga ggacaagtcc aaggagaata gcagagctac
     6061 cttcaagacc tgcaaggaag ttattcatgc ctgtgttgat agctgccatt gggatgttaa
     6121 cgaagaacat caagaaacca gtgattgctg ttcccaaaag tggcaagagc aagatagcgt
     6181 tcaaaccttg aagtgatttt ggaaggttgc gcaaagcgtt gcgaagaacg agcactacac
     6241 caccagcaag gaaaccacca acaagggcac ccaagaaacc agatgataca cctgcaagag
     6301 caagcgtttc ttcaccacca gctgcgtaag gaattttacc gaacgcaaga ccgctactag
     6361 caattgcacc agctacgaaa cctgcaacca aacctggttt ttcagcgatt gagtaagcaa
     6421 tgtaacctgc tagaagtggc aacatgaagc cgaaggctgc tccaccgatc ttcatgaaca
     6481 tagctgcgat ttcatggtat gaaccaagac taccaaggct gtcttgtggt acacctaaag
     6541 ctccatcgaa taggaaggca agggcaatca agataccgcc accgataacg aatggcaaca
     6601 tttgagaaac accactcatc aagtgtttat agaacgcttt gccaagactg agtttttctg
     6661 aagattccac agtagctgct ccttctgacg ctgtgtaggc tgctgcttta ccgtttaaga
     6721 tgatgttaat caattcttct ggttgtttga taccagctgc aactggacga gataccaatg
     6781 gtttgccatt gaaacgaggc atgtcaacag ccttatctgc tgcgatgata acacccgccg
     6841 cattcttgat gtcttcagca gtcagtttgt taccaacccc agacgcaccg tttgtttcaa
     6901 ccttgatgcc aacacccatc tcagcagcct gcttcttaag agcttcttct gccatataag
     6961 tgtgagcaat accagttgta catgctgtaa ctgcaacgat aaatggacgg tcgccactag
     7021 gagctgcaac cacttcttct actgccttgt cagctgcttc agctgtatcg aagactgcaa
     7081 tcacttcttc aggatttgtt gccgcacgaa gtctatcagc aaatccagct ttcatcaagt
     7141 atttagacaa ttccgcaagg gctgccaagt gcgtgtcgtt ggcaccttct ggagctgcaa
     7201 tcatgaagaa caagtcagtt ggttgaccat caagacttgc ataatcaaca ccattgtttg
     7261 attttgcaaa gagaacagtc gcttctttta cagccgcatt cttagcgtgt ggcattgcga
     7321 taccgtcacc aagtcctgtt gtcgtttgtg cttcacggtt catgatacct gttttgaaaa
     7381 catcaaaatc cgttacataa cctttatcaa ccaaactagc aatcatttca tcaataactg
     7441 cttccttgct tgtcgcttgc aaatcaagca acatgacatc ttttctcaaa acgtcttgaa
     7501 ttttcatagt gtttctacct caactttttc ataagtttcc ttaataaatt cgatacttgc
     7561 caaatcatct gaaaaggctg ttgccgtacc gcaagcaact ccccatttta gggcttcaat
     7621 tgggtcttgt gagcgaacgt attcgccagt gaatcctgcc accatggaat ctccagctcc
     7681 gacagagttt ttcactgtac ccttgattgg tttggcaaag taagtagctg ctggagtgac
     7741 caacaaagcc ccatcccctg ccatggaaat gataacattc tgagcaccca tttctaagat
     7801 tttcttagcg taggtctcga tatctgcaat gtcattcaac tcgacgttga agattgcttc
     7861 aagttcatgg ttgtttggct tgaccaagag tggctggtgt gctagtgagt ctagaagtgt
     7921 ttgaccttca aagtcacaga ccacttgcgc tcctgctttt ttagccacag gaatcagcct
     7981 attatagact tcatttccaa gactagctgg agcagaccca gcaaagacaa cggtgtcttc
     8041 tttggtcaat cctgctaaga ccatttctaa ttcggccaac tgtgtatctg tcacaatcgg
     8101 acccaaaccg ttgatttctg tttcttggtc agctttgatt ttcacgttaa tacgtgtatc
     8161 ttgatcaacc tgaacaaaat cagtatcaat tccttcggca atcaatccat ctttgatgaa
     8221 ctgacctgtg aatcccccaa ggaaacccgt tgctgtatta ggatagccca atcgcttcaa
     8281 aacacggcta acgttaattc ccttaccgcc ggcatactta tcgtcgctct ccatacgatt
     8341 gacactgccc gtctcgacat gttctaaacg aacgatatag tcaatcgctg gattgagcgt
     8401 aactgtataa atcatacctc gattaccctc gttttctctt ttatgatgtc caacaaactg
     8461 ctttctgaag attggcagat aatgttggat ttttcgattg ccgcaacttt aacaaaggag
     8521 aattgaccaa ttttagaagc atctgccaaa acatagcttt ctttagcatt gtctatgata
     8581 gtgcgtttga tggtcgcttc ttcaatatct ggcgttgtaa aatagttttt atctatacca
     8641 ttcatcccca gaaatgcctt atcaaaattg agctgtcgaa tttgttccaa tgccactgct
     8701 ccaacggaag catctgttga ttgcttaaca aaaccaccta taatgattgt ttttatcttg
     8761 cgttcaacca gcctcactgc gtggtgaata gaattggtaa cgactgtcag attttcctgc
     8821 tgaagataat caatcagtac accggttgtt gtacctgcat ctaaaaaaat tacgtctccg
     8881 ttaacaattt gttgagcagc tctttccgca atctctcgtt tttcttgaac gtttttgaca
     8941 gatttttcta gaatagactc ttcgagttgc aaattccttg gggcttcagc accgccgtgt
     9001 acacgacgaa gcttaccttc gttttccaac tcgtccaaat ccctacgtac agtagactca
     9061 gatgtatcaa gaacttcgat taattcctct agccgaacaa actgttgaga gtgtattcgc
     9121 tccaatataa tctgttttcg ttctgatttg agtatgttca acacctcctg caatcgttta
     9181 caaaaatcat tatacacgat tttctgccat tgtcaatcat tttctatcat tttctttcaa
     9241 aaaaacaaaa aaactagcag aaatctacgc ttccactagt ttctcaatca tttttttcat
     9301 catatccatt tcttctggac gaccaataga taggcgaata tgcccctcta atttacctgt
     9361 tggataatat ttgaaattcc aatcctgtgc caaggcttgc tgataaaatt cttctgccca
     9421 tggtgcttta aacgttacaa aactagcctg actaggtaga acttgacagt tatctaaccc
     9481 ttgcaagaat aaaatcattt cttctcgcaa attctgaatt actcccactg tttctaaaac
     9541 cttgtctgta taattcaatg caacggctgc catttttgca gtcaagtttg acaagctgaa
     9601 tgggggaatg accttgtcca attcataaat taactcttcg cttcctaccg caaagccaac
     9661 tcgtaaacct gcaagtccaa aagcctttga taaggtccgt aaaactaaaa cattgtcaaa
     9721 ttgttgcaga cgactgatga agctttcgac atttgcaaat tcaacataag cctcatctac
     9781 aacgagtagc cctttaaaag aaatagccaa tttctccaaa tcttctagtg aataagctac
     9841 tgaagaagga ttgttaggat tggaaagcat gattatcttt gccttcacct cctctgcacg
     9901 cgccaacaat ttctctactg gcaggacgag cgtatcgttt tttgcttcca aatcaatggt
     9961 ttcaaaacgg ctaccatgca tgtgattata ggtttcatac atgaagaagt ctggattgac
    10021 agtcaaaaaa gtatcatcct tttccataaa ggtggaaaca atcatgtgaa taaggtgatc
    10081 agagcccaca cctactgtta cttgtttggg attcactcca agataggccg catatttctc
    10141 gatcagttcg gagtaagtgt tatcaccata aaaacttaat tgattgacat ccaaattttc
    10201 caaggtctct tgtggtaaaa aggaccaatc aataatgcga ttttcattat ttcccaagtc
    10261 aaacctagcc ttttgtttga ttttataagg ttggtaatgt tcaaatacct tgcgtttcat
    10321 catttctcct tctagcaaga aaaacacagt ctaatcaact gtgtcttctg ccttgatttt
    10381 ttcaaaaata gttaactctt cagctgtaaa gttgtagttt tccaacaagt ctggacgacg
    10441 ttgataggtt ttgcgtagac tttcttccaa acgccattta cgaatatttt catggtgacc
    10501 gctcattaat acatctggaa ctaccattcc acgatactcg tatggtcggg tgtattgagg
    10561 atattccagt agaccagatg aaaaactatc atccgtatga ctgacttctt tgccaataac
    10621 ctctggaatc agacgaacgg tagcatcaat catggtcata gctgccaact ctccacctgt
    10681 cagaacatag tctccgagag aaatctcatc tgttaccaag gtcttaatcc gctcatcata
    10741 tccttcatag tgaccgcaaa tgaaaatcag ctgctcttcc tgtgccaatt cttcggcata
    10801 ggcctgattg aaagtacgcc cagcgggatc caataggata acacgtggct tggcttgttc
    10861 aatgctatcc atggtatcaa aaattggctg ggcacggagc aacatgcctt gtcctccacc
    10921 atagggttca tcgtccacat ggcgagcctt ttcagctttc tctcggaaat tgtggtagtt
    10981 gatttctagc aagcccttgt cacgcgcctt acccacaata gagtgttcta gaggtgcaaa
    11041 catctcaggg aagagggtca aaatatcaat tctcatcgtc taggccctcg agcaattcca
    11101 catcgacacg gttatttgga atatcaatgt tcaagacaac aggtggaata tatggcaaga
    11161 gcaggtcttt cttacctttt cgtttcacca cccagacatc attggcacct ggctgcaaga
    11221 tttccttgat ttgtccgatc agaatatcat tctcatagac atccaagccg ataatttcat
    11281 ggtaataaaa ctcaccatct tcaaggtcag tcaagttttc ctcggcaatt ttcaaactaa
    11341 agcccttgta cttttcaata tcgttgatat ggtacatgcc cttaaacttg ataatatcaa
    11401 agttcttagc cttacggtga ttagcaattt ccacttccat gacaaactga tccttgtcat
    11461 caaaaagtgc aagctttgcc ccttttttaa aacgttcttc tgcaaaatcg gtcacagaca
    11521 aaacacgcat ttccccttgt aggccttgcg tgttaacaat ctttcctacg ttaaaataat
    11581 tcataatttc tccatttgat tttctatcct tttattttaa catgagcaag atattttcac
    11641 aagagagaaa aagccggatg tctccgactt tcatagttaa tttatttcac ggacaaatga
    11701 gaatgagcta ttatgatcaa gcgcaaatta agagctattc cgcagacaaa aatccctgaa
    11761 ataagaccga tccaataggc ttctggaccc atttggaaaa atctttcaag caagaatgcg
    11821 actgggaagg tcatggacca ataagcaacc aaaccaagta taaatggaac agtcgtatcc
    11881 ttgtaaccac gtaaaatccc ctgaataggt gctgtaaagg catctgctaa ctggaacatg
    11941 agggcaaagc tcaggaaatg cgaggtcaaa cggataaatt ctgcgtcatt accataaagg
    12001 taggcaactt tggagcggaa aaaatatagg aaagtgagtg ttaggactgc aaaaccaagt
    12061 gcaaccaagc gtcctagacg gctgtaggct ttcacatcct gtggtcgttt agcccctact
    12121 tcataagcaa tgacaatagc tagggctgaa gaaactgagg caggaaaggc atataataat
    12181 gtcgcaaagt tcatagctga ctggtgggct gctataatct gtgctgaaaa ttttgccatg
    12241 tagagcccta ctactgcaaa aattgctact tctgcaaaga cttgtaaacc gatgggaaga
    12301 ccgattttca aaccttcctt caataaagaa aggtcaatcg gactccactg ccaaatctga
    12361 taagatgcaa tagtcttatt tttgcacatg acaatgacga ttactagcaa taccgcccag
    12421 taagctaaag cagtacccaa tccagctcca gctccgccca gggccggtag gcccattttc
    12481 ccataaatta agagatagtt aaatagactg ttaaatggga caaggagcaa catgaggtac
    12541 atggatagct gagtcaaacc gagagcgtcg aaaaaagagc gacagacact aaagagcaag
    12601 agaggcagga tgccaatgga aataaaatag aggtactgcc gaccaacttg aaaaatagat
    12661 tcatcaagtc caaactgagc caaggcggga acagcgataa ataggaccga gcacaagagt
    12721 aaaatggtta atccgatggc tagatagaca aactgatgaa attcctcacg aatcttatct
    12781 ctttcacctt ttcctaaata ttgagcgatg attgggacca acgcagatac aatccctgtt
    12841 agaaatgaaa aaagcggatt ccaaagactt gttgccatgg aaacacctgc caaatccatg
    12901 gtactatact gaccagtcat catagtatca atgaaggagg ctgaataatt agcaaattga
    12961 taaattaaaa taggcaaaaa tattttgata aagagccaga gcttctcttt
//
LOCUS       DBNFEG010000043        12818 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.043, whole genome shotgun sequence.
ACCESSION   DBNFEG010000043 DBNFEG010000000
VERSION     DBNFEG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12818
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.043"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..495
                     /locus_tag="AC1PNG_000855"
     CDS             <1..495
                     /locus_tag="AC1PNG_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIP6701368.1"
                     /translation="GIGKTTLANILHKRIENSYLYDPELLGDFLQHQLPKTVCPEDFQ
                     DYSVWRRSTYKIVFDLATKTDKIIIIPMTIYKKEYYQEITQQLIQDKIPLEHYILLAD
                     KTTILERLDNRVNEDNIWAKRHLDVCLKAFESHIPGQRLNTDSLKPEEVAEEILMLSE
                     FTKK"
     gene            662..1732
                     /gene="xerS"
                     /locus_tag="AC1PNG_000856"
     CDS             662..1732
                     /gene="xerS"
                     /locus_tag="AC1PNG_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HIP6701369.1"
                     /translation="MRRELLLEKIDQLKEIMPWFVLEYYQSKLAVPYSFTTLYEYLKE
                     YRRFFEWLQDSDLVSVERIADIPLDILEHLTKKDMEAFILYLRERPLLNANTTQNGVS
                     QTTINRTLSALSSLFKYLTEEVENEQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETMEFLDYVDKEYQVNLSKRALSSFQKNKERDLAILALLLASGVRLSEAVNLD
                     LRDVNLNMMIIEVTRKGGKRDSVNVAGFAKPYLEAYMSIRQQRYKAEKTDTAFFLSEY
                     RGLPNRIDASSIEKMVAKYSADFKIRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAN
                     TQVTDLYTHIVNDEQKNALDQL"
     gene            complement(1802..3310)
                     /locus_tag="AC1PNG_000857"
     CDS             complement(1802..3310)
                     /locus_tag="AC1PNG_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HIP6701370.1"
                     /translation="MSHHHVLQNGSDIRGIAIATDEYAVNLTPQATQEVVRGLIHWLT
                     QKPELAQTYQKGQLTIGIGRDSRLSGPDLVAAFTEEAVRLGVQLIDFGMATTPALFMS
                     TQYPQFKCHAGVMITASHLPHYFNGIKIFSENGGAEHEDIDFILSSSEDLPASDLGSV
                     TKADLITPYAQDLVGKIRTACGGQEKPLTGLNIIVDAGNGAGGFFAEKVLAELGADTT
                     GSQFLDPDGTFPNHVPNPDNKEAMESIRQAVLKQGADLGIIFDTDVDRAALVTKSGEI
                     LNRNNLIAVLSQIVLAEHPGTSIVTNSPTTEHLKVFIESLGGKQIRYISGYRNVINRA
                     ILANQEGVDCQLAIETSGHAAFKENYFLDDGTYVAAKILMLLPKLQAEGKSLDDLIAQ
                     LKQPLETQEVRFKLEAAGYRALGEQVIADFRNTSLEGFAFNPENEEGVRFDLTELYGD
                     GWLLLRMSLHEPLLVLQVENDQTGHIPAVLRTLSAFLDQYPAVNQEKLKALI"
     gene            complement(3386..4327)
                     /locus_tag="AC1PNG_000858"
     CDS             complement(3386..4327)
                     /locus_tag="AC1PNG_000858"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIP6701371.1"
                     /translation="MIAKFIREYRSFYFSYIVLATSFILLFFLYQLPTEFLQNALLLS
                     LTLLILLTVGLFWKFRNRMRALEDYVHEEALPLLNKPVDLAYLNLIEAEKEATREQLL
                     VYKSREEDLQAMVKMWSHQMKVPLAALSLMSQTDNLNQQDVNHQLLRLDNYMANLLNY
                     LKLTNHASDFRFEQVEVREVVVDLVKKYRNQFLQKDISVTIDGNWLLKSDRKWLSFAL
                     SQIIDNALKYNKTGGKVAIELNEGISISDTGIGILAEDIPRLFDEGFTGFNGREHQKA
                     TGFGLYLTKRVLNQLELAISVESQLEVGTSVTITKVQ"
     gene            complement(4324..4989)
                     /locus_tag="AC1PNG_000859"
     CDS             complement(4324..4989)
                     /locus_tag="AC1PNG_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HIP6701372.1"
                     /translation="MKKEKIYIVEDDEMIVQLLKQHLGKSYQVESVQNFRAVSQEVAE
                     IKPDLVLMDISLPYYNGFYWTTEIRKTMTMPIIFISSSDDEMNAVMAMNMGGDDFVSK
                     PFSLGILDAKIGAFLRRVNQFSKSTDLSIDQFQLSLDGRFSNELEQVQLSPTETKILS
                     ALLERQGQIVSKEELLEKLWENEEFIDQNTLNVNMTRLRKKVSEVGFERIHTVRGVGY
                     LVK"
     gene            complement(5028..5285)
                     /locus_tag="AC1PNG_000860"
     CDS             complement(5028..5285)
                     /locus_tag="AC1PNG_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE family
                     toxin"
                     /protein_id="HIP6701373.1"
                     /translation="MSYKVILSAKAQKQIRKMDKTAASLILRYLYKHIDGCDNPRQYG
                     KALTANRSGQWRYRIGNYRVIVNIEDDKLIVTSIKMRNGSI"
     gene            complement(5275..5514)
                     /gene="relB"
                     /locus_tag="AC1PNG_000861"
     CDS             complement(5275..5514)
                     /gene="relB"
                     /locus_tag="AC1PNG_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HIP6701374.1"
                     /translation="MAAITLKVSEQDKLFMQAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADVAWAEYQADLANRIEPMTLEQMAEELGIEL"
     gene            complement(5606..7576)
                     /locus_tag="AC1PNG_000862"
     CDS             complement(5606..7576)
                     /locus_tag="AC1PNG_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIP6701375.1"
                     /translation="MFYLKLAGQNIRKSIGVFAPFVLASLVLFTLICSMFLLMLSPVM
                     KTMGTGAVSIGLGVVVLTIFSVIMEIYSFNFLMKQRAREFGLYNMLGMNKKKVALIAS
                     IELMMIFLLVVVLGSVLAGVFANVLYLIFVNITHYSDLHFGIEPLAFVMTAFLFAGFF
                     FLLELLAIRTIGKTSPLILFRASEKGEREPKGNVLLAALGVLSLGMGYYLSLSSKAEG
                     LAVIYRFFIAVLFVIAGTYLFYISFMTWYLKARRKNKRYFYTPEHFITTSQMIFRMKQ
                     HATGLANITLLAVMAFVTIATTTSLYTGMANMTSGLYPKETSITYKVSYRSQGVAAYQ
                     QSVLSHFPEKANDNLSYLTYQAGLVYDGGSDIVINPETIANPDFSKMAFTYFITQDDF
                     RKLGNDLPELAANQVAFMRPSEMPSLKKLVLGDQTFENVANLDQAIFPDITNTLNAAV
                     MVVSDDTVLDAVREYYESNNPQGYTVSLDYRVFTDLTDEEVASIGQQTGDSYNINDAN
                     GEFLGYAMQKSDFNNLLMGFTGSFLFTGFLLGISFLLGAALIIYYKQYSEGHEDKKSY
                     KILQEVGMSQQAVKKTINSQTLLVFFMPLAMATLHFIVALVMLKQMLMVFGVVSSSMI
                     YTVSGITLLAVVLIYFVIYKWTSRTYYRIIER"
     gene            complement(7577..8329)
                     /locus_tag="AC1PNG_000863"
     CDS             complement(7577..8329)
                     /locus_tag="AC1PNG_000863"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6701376.1"
                     /translation="MLLEINHLEKVYRTRFSKEETRALQDVDFKVEEGEFIAIMGESG
                     SGKTTLLNILATLDKPTRGSVILNNQDITKIKESKLADFRLRNLGFVFQDFNLLDTLS
                     IQDNIFLPLVLARESYEEMEKRLKVLAPKLRIQDLLKKRPFELSGGQKQRVAIARSLI
                     TQPQIVLADEPTAALDYRNSEDLLNLFEDINLDGQTILMVTHSANAASHAKRVLFIKD
                     GRIFHQLYKAGKSNQDFAKEISLNMSALLGGE"
     gene            complement(8346..8729)
                     /locus_tag="AC1PNG_000864"
     CDS             complement(8346..8729)
                     /locus_tag="AC1PNG_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NisI/SpaI family lantibiotic immunity
                     lipoprotein"
                     /protein_id="HIP6701377.1"
                     /translation="MKKKVFGLLALGSIILTACSFSSAGLSFDEGYQTFHYKGKDYAV
                     SKQEVAENNIQEVEVKFMEWPVVKEEGAVKKTVSLNNLYITTSKEWAIGVQDGYYKVD
                     LEDNIAESDRIDYQAVLDQVDLDSE"
     gene            complement(8742..8882)
                     /locus_tag="AC1PNG_000865"
     CDS             complement(8742..8882)
                     /locus_tag="AC1PNG_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701378.1"
                     /translation="MSFKFLQVKRMEEKAFTEKEKTELLEQAEFLEELALDLQTMRFM
                     AY"
     gene            complement(8981..9715)
                     /locus_tag="AC1PNG_000866"
     CDS             complement(8981..9715)
                     /locus_tag="AC1PNG_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HIP6701379.1"
                     /translation="MKSYNAYQLKLAMAGLMVLDHLKIIPDFIPDQLALVFHLATRCV
                     AVFFAYMLVEGFLHTRNVKAYLLRLYGAAGLMATGNTLLNSLYQSKGIYISNNIFLTL
                     AVGLTLLICLQNFSEHTRAKQIIQVLLAGLLTIIGALFTEGGTVVLPFILITYLGRES
                     ATKRTIAYGFLALVLLAMSYQPYERLEMTIQMMLYNADWLFILVLPILSLYNGQAGPR
                     TVFSRYFFYIFYPLHLWILATIGYFL"
     gene            complement(9728..9865)
                     /locus_tag="AC1PNG_000867"
     CDS             complement(9728..9865)
                     /locus_tag="AC1PNG_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701380.1"
                     /translation="MSFKFLQAKQIFGKSQESKRLEEQDKQAEFLFELEHELFQRNWM
                     Y"
     gene            complement(10004..11170)
                     /locus_tag="AC1PNG_000868"
     CDS             complement(10004..11170)
                     /locus_tag="AC1PNG_000868"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIP6701381.1"
                     /translation="MNYLVISPFYPENFQPFTIELAKKEGVTVLGIGQEPYDQLPEEL
                     RNALTEYFRVEDLENIDEVKRAAAFLIYKHGPIDRVESHNEYWLENDAALREQFNIFG
                     AKPKHLKKTKFKSKMKKYFKKAGVPVVPGVVVKSERDFGRALKNVGFPMIAKPDNGVG
                     ASGTFKIENKEDFDRFVETWDGATIYFLEKFVNSSIITTYDGLLDHEGNVVFETGLTY
                     VHTPLELMLSRKDNAYFIEKELDPQLVKYGRAIIKAFGMRERFFHIEFFKDGDDYIAI
                     EYNNRMAGGFTVEAYNYAHSIDLFRDYANVATGGTVEERRFEPQYCLVATRRDTTEYV
                     HSADDIHQKFAGKIKTVKRMPDAFAELQGNDAYLLVTESKKELDEMIAYIGLTK"
     gene            complement(11189..11935)
                     /locus_tag="AC1PNG_000869"
     CDS             complement(11189..11935)
                     /locus_tag="AC1PNG_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002909732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HIP6701382.1"
                     /translation="MHVEFLSHWSGNLGREMNLNRYGHAGIPVVVFASSGGSYNEYAD
                     FGMIEACRGSIEAGQVQFFTLTSYDSESWLADWKSIHDKAEAHRAYERYVIEEAIPFI
                     KHKTGWFDGMMTTGCSMGAYHALNFFLQHPDVFTKVIALSGVYDARFFNNNHDYGHDD
                     AVYQNSPSDYIWNQNDGWFIDKYRQADIIVCTGLGDWEQDGLDSFYNLKRAFEEKNIP
                     AWFDTWGTDVAHDWVWWRKQMPFFLHSIGL"
     gene            complement(11966..12793)
                     /locus_tag="AC1PNG_000870"
     CDS             complement(11966..12793)
                     /locus_tag="AC1PNG_000870"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIP6701383.1"
                     /translation="MNKSYFYLDMRTHELDVPFTGRKRRVRVLLPKGYAENTEETYPV
                     VYFHDGQNVLYSKEAFSGHSWKVIPTIKRNPDIAKMIVVAIDNDGADRMHEYAAWKFS
                     EMGIPGVQFGGKGTLYAEFVMDVVKPFIDKEYRTKSDKVHTAMIGSSLGGNITQFMGL
                     AYQNQIGCLGVFSSANWLHQDAFDRYIERQQLDSEQRVYIYVGTEEADDTDKTLMAGN
                     IKQAYINSSLTYYRQLIAGGVDLDNIALEVVSGAVHNEEAWALYLPACLRFLSEHWS"
BASE COUNT         3696 a         2851 c         2592 g         3679 t
ORIGIN      
        1 ggtatcggaa agactacttt agcaaatata ttacacaaac gaatagaaaa ctcatatctc
       61 tatgatcccg agttactagg tgatttctta caacatcaat tacctaagac tgtatgtcct
      121 gaagattttc aagattattc cgtttggcga cggagtactt ataaaatagt atttgattta
      181 gcgactaaaa ctgataagat tattattatt cccatgacaa tctataagaa agaatattat
      241 caggagatta ctcagcaatt gatacaggat aagattcctt tggaacatta tattttgtta
      301 gctgacaaaa caaccatttt ggaacgttta gataatcgag tcaatgaaga caacatctgg
      361 gccaagagac atcttgatgt ttgtttaaaa gcttttgaaa gtcatattcc ggggcaaagg
      421 ttaaacacag acagtttgaa gccagaagag gttgcggagg aaattctaat gctaagtgag
      481 tttaccaaga aataatgtag acttttggga ttttgggtgt gaaaaaaaat ttccagttat
      541 ttatgttttt tggttatccc tctgatatcc ccacaaacat ctttccgaaa aactataatt
      601 ttcttgaaac ttatgtaaga ttatgctata ctactcatat agctaaaatc aggagaaaat
      661 gatgagacgc gagttattac tagaaaaaat tgatcaacta aaagaaatta tgccttggtt
      721 tgttctggaa tattatcagt caaaattggc tgtgccttac agttttacta ccttatatga
      781 atacttgaaa gaataccgtc gattctttga gtggttacag gattcggatt tggtatctgt
      841 ggaacggatt gctgacattc ctttggatat tctggaacat ttgacaaaaa aagatatgga
      901 agcctttatt ctttatctgc gggagcgtcc cttgctgaac gccaatacca cgcagaatgg
      961 tgtgtcgcag accaccatta accgtaccct gtcggctctt tctagtctct tcaagtattt
     1021 aaccgaagaa gtggaaaatg agcagggcga gccctacttc taccgcaatg tcatgaaaaa
     1081 ggtatctacc aagaagaaga aggaaacctt ggcagcgcgg gctgagaaca tcaagcaaaa
     1141 gctctttttg ggcgatgaaa ccatggagtt cttggactat gtggacaagg aataccaagt
     1201 caatctctct aaacgtgccc tctcctcttt ccagaaaaat aaggagcgag atttggcgat
     1261 tctggcactt ctcttggctt ctggcgtccg tctgtcagaa gcggtgaatt tggatcttcg
     1321 agatgtaaat ctcaatatga tgattatcga agtaacccgt aagggtggta agcgggactc
     1381 ggtcaatgtg gctgggtttg ctaagcccta tctggaagcc tatatgagca tccgtcagca
     1441 acgctacaag gctgaaaaaa cggatacggc cttcttcctg tccgaatacc gtggtctacc
     1501 caatcgtatc gatgcttctt ctattgaaaa aatggttgcc aagtactctg cggacttcaa
     1561 gatacgcgta accccccaca aactccgtca taccttggca actcgcctat acgacgccac
     1621 aaagtcgcaa gttctggtca gtcatcagct gggccacgcc aatactcagg tcactgacct
     1681 ctacacccat atagtcaatg atgagcaaaa aaatgcactg gatcaattat agtgttacgt
     1741 aatattaatt atgtaaataa attaaggagt aagcgaaaac ttaccccttt ttctcttaaa
     1801 tctaaatcaa tgctttcaat ttttcctgat tgacagctgg gtactggtcc aaaaaggctg
     1861 agagagttcg taaaacggca ggtatgtgtc cagtctggtc attctccacc tgcaagacca
     1921 agagtggctc gtggagactc atacggagca agagccagcc gtcaccataa agctcagtca
     1981 ggtcaaaccg cactccttct tcgttttctg ggttaaaagc aaagccttcg aggctggtgt
     2041 ttctgaaatc agcaataacc tgttcaccaa gtgctcgata gcctgccgct tctagtttga
     2101 accgtacttc ttgggtttca agtggttgct tgagctgggc aatcaagtcg tccaaggact
     2161 tgccttccgc ttgcagtttt ggtaagagca taaggatctt agccgctaca taggtcccgt
     2221 catcgaggaa gtaattttcc ttaaaggcag catgtccaga tgtttcgata gccaactggc
     2281 aatcaactcc ctcttgattg gctaaaatag cacggttgat gacattacgg tagcctgaaa
     2341 tatagcgaat ttgtttgcct cccaggcttt ctataaagac cttaaggtgt tcagtcgttg
     2401 gggaattggt cacaatgctg gttcctggat gttcagctag gacaatctgg ctgagaacgg
     2461 caatcaggtt gttacgattg agaatttctc ctgacttggt aaccagggcg gcacggtcaa
     2521 cgtcggtatc aaagataatg cctaggtctg caccttgctt caagacagcc tgacgaatgc
     2581 tttccatagc ttccttgtta tctgggtttg gaacatggtt tgggaaggtg ccgtctgggt
     2641 ctaggaattg tgaaccagtg gtatctgctc ccaattctgc caaaactttc tcagcaaaaa
     2701 atccgcctgc accgtttcca gcatcgacga tgatattaag acctgtcagt ggtttttctt
     2761 gtccaccaca ggcggtacga attttgccaa ctaagtcttg ggcataaggc gtaatcaaat
     2821 ctgctttggt cacacttcca aggtcagaag ccggcaagtc ttcactactg gagaggatga
     2881 agtcaatatc ttcatgttct gctccaccat tttccgaaaa aatcttaatg ccgttgaagt
     2941 aatgcggcag atggctggca gtgatcatga caccagcatg gcacttgaac tgaggatact
     3001 gggtgctcat gaagagagct ggcgtggtag ccataccaaa gtcaatcagc tgaacgccca
     3061 gacgaacggc ttcttctgta aaggctgcga ccaaatctgg accgctgagg cggctgtctc
     3121 gaccgatacc gatggtcaat tggccttttt gataggtctg cgccagttcc ggcttctgag
     3181 ttagccaatg aataagtccg cgaactactt cctgggtggc ttgcggagtg agattgacag
     3241 catattcgtc tgtagcgata gcaattccac gaatatctga tccattttgc aagacgtgat
     3301 gatgggacat agtaacctcc taattcaaac taaatttagt atatcatatt ttcaaagcgg
     3361 tttcaaaatg aaaaaagcac agcttctact gtacttttgt gatggttacg ctggtcccaa
     3421 cttccagttg actttccacg gaaatagcta gttccaactg attcaggacc cgcttggtca
     3481 gatacaatcc aaatcctgtc gctttttggt gttctcggcc attaaaacct gtaaatccct
     3541 cgtcaaacag gcgtggaata tcttctgcca gaatcccaat tcccgtatcg gaaatggaaa
     3601 ttccctcatt cagttcaatg gctacctttc caccagtctt attgtacttg agggcgttgt
     3661 cgattatctg tgacaaggca aagctgagcc attttctgtc ggacttcagt agccaatttc
     3721 catcgatggt cactgaaatg tctttttgta agaactgatt gcggtatttt ttgaccaaat
     3781 ccaccaccac ttctcttact tccacctgct caaatcgaaa atcgctggca tggttggtca
     3841 gcttgaggta gttgaggaga tttgccatat aattgtccaa gcgtaagagc tggtgattga
     3901 catcctgctg gttgagattg tcagtctggc tcatcaaaga cagggcagcc agaggcactt
     3961 tcatctggtg ggaccacatt ttgaccatgg cttgcaggtc ctcttctcgt gacttataga
     4021 ccaaaagctg ctctcgtgtc gcctccttct ccgcttcaat caagttgaga taggcaaggt
     4081 ccacaggctt attgagcaag ggcaaggctt cctcatgaac atagtcttcc aaggctctca
     4141 tgcggttgcg aaacttccag aaaagcccta cagtcagtag gattaaaagg gttagggaca
     4201 atagcagagc gttttgcagg aattcggtgg gcagttgata gagaaagaaa agcaagataa
     4261 aactagtagc cagaacaatg taggagaagt aaaaactccg gtattcacgg ataaattttg
     4321 ctatcatttg accaagtacc ccacgccacg aaccgtatga attcgttcaa acccgacttc
     4381 gctcaccttc ttacgcagtc tggtcatgtt gacattgagc gtgttctggt caataaattc
     4441 ttcattttcc cagagttttt ccaggagttc ttccttgctg acaatctgcc cttgtctttc
     4501 tagtaaggcg gacaaaatct tggtttccgt aggagacaat tgaacctgtt ccaattcgtt
     4561 tgaaaatcgc ccgtctaaac taagttggaa ctggtcaatt gacaaatcag ttgatttgct
     4621 gaattgatta acccgacgca agaaggcgcc gattttggca tccaaaatcc ccagagaaaa
     4681 cggcttagac acaaaatcat cgccacccat gttcatggcc atgaccgcat tcatctcatc
     4741 atcgctagag gagataaaga taatgggcat ggtcatggtt ttacggattt ctgtcgtcca
     4801 gtaaaaaccg ttgtaataag gcaggctaat gtccatcagc accaagtctg gtttgatttc
     4861 agcgacttcc tgactgacag ctcggaaatt ctgcacactt tccacctgat acgattttcc
     4921 caaatgctgc ttcaagagct ggacaatcat ctcatcatct tcaacaatat aaatcttctc
     4981 ttttttcatc ttcttcattc tgcaaaagcc aggacacagg tcttggctta tatacttcca
     5041 tttctcattt ttatgcttgt cacaatcaat ttatcatctt caatattaac gataaccctg
     5101 taattgccga tacgatagcg ccattggcca gaacgattag cagtcaaggc ttttccgtat
     5161 tgtctagggt tgtcgcaacc gtcaatatgc ttgtagagat agcgcaaaat caaacttgca
     5221 gcggttttat ccatctttcg aatttgcttt tgggccttag cagacaaaat aaccttataa
     5281 ctcaatgccc aactcctcag ccatttgttc taatgtcatc ggctcaattc tattcgccaa
     5341 gtctgcttgg tattctgccc aagcaacgtc tgccacacgc gcatcgtact cgtcttcaag
     5401 ggcttcaagg gttttagtcc gaatgagttc tgacaaactg acaccttcaa actttgccat
     5461 agcctgcata aagagcttgt cctgctcaga aactttcaat gtaatagctg ccattttctt
     5521 acctctttga gtattccttt ggtattacca ttgtaacacc atttccctga aaaagcaaga
     5581 aatctcgcac aaggcgagac ttctgctatc gttcaataat acgataatag gttcggctgg
     5641 tccacttgta aatgacaaag tagatgagga caacagcaag caaagtaata ccactaaccg
     5701 tataaatcat gctggaagaa actacaccga acaccatcaa catttgcttg agcatgacta
     5761 gtgcgacgat aaagtgcagg gtcgccatag ccagtggcat aaagaagacc aagagtgttt
     5821 gagagttgat ggttttctta acagcctgct ggctcatacc cacttcctgc aaaatcttgt
     5881 aggatttctt gtcctcatgt ccttccgagt actgtttgta gtagataatc aaggctgcac
     5941 caagtaggaa actaatgccc agcaagaaac ctgtaaagag gaaactgcct gtaaagccca
     6001 tcagtaaatt gttaaaatct gatttttgca tagcatatcc taggaattct ccgtttgcat
     6061 cgttgatatt ataagagtcg ccagtttgtt gaccaatcga agcaacctct tcatcagtca
     6121 agtcggtaaa gacacggtag tccagactga ctgtataccc ttgcggatta ttggactcgt
     6181 aatactcacg aacagcatcc aacacagtgt catcactgac taccataacg gcggcattta
     6241 gcgtattggt gatgtctggg aaaatagcct ggtcaagatt ggctacattc tcaaaggttt
     6301 gatcgcccaa aacaagcttc ttaagcgacg gcatttcact cggacgcata aaggcaactt
     6361 gattggcagc cagctctggc aggtcattcc ccaatttcct gaaatcatct tgggtgatga
     6421 agtaggtaaa ggccattttg ctaaagtctg gattggcaat ggtttctgga ttgatgacaa
     6481 tatcgctccc accatcatag accaaacctg cttggtaggt cagataggat agattgtcat
     6541 tggctttttc tggaaaatgg ctgagcaccg attgctgata ggcagctaca ccttgacttc
     6601 tatacgaaac tttataggta atagatgttt ccttgggata gagtccactt gtcatatttg
     6661 ccatacctgt atagagggat gtcgttgtcg caatggtcac aaaggccatg accgcaagta
     6721 gcgtaatatt agccaaaccc gttgcgtgct gcttcatccg gaaaatcatc tgcgaagtcg
     6781 taatgaaatg ctccggcgta tagaaatacc gcttgttttt ccgacgagcc ttcaaatacc
     6841 aagtcataaa actgatgtag aacagatagg tccccgcaat gacaaatagc acagcgataa
     6901 agaaccgata gataacagct agaccttctg ctttcgatga aagcgatagg tagtagccca
     6961 tcccaagact aaggacacca agagcagcta gaagaacatt tcctttcggc tctctctctc
     7021 ctttttcact agctcggaac aaaatcagcg gactggtctt accaatggtt cgaatggcca
     7081 gcaattccaa gaggaaaaag aaaccagcaa agagaaaggc agtcatgaca aaggccagag
     7141 gctcaattcc aaaatgtaaa tcactataat gtgttatatt gacaaaaatc aagtagagaa
     7201 catttgcaaa cacaccagct aggacagaac caagaaccac aaccagtaag aatatcatca
     7261 tcaattcgat gctggctatt aaggctactt ttttcttgtt catccccagc atgttataga
     7321 gaccaaattc cctcgcccgt tgtttcatca ggaaattaaa actataaatc tccataatga
     7381 ctgagaaaat ggtcagaaca acgacaccca gcccgataga cacggcacct gttcccatgg
     7441 ttttcatgac tggactaagc atgagcaaga acatagaaca aatcaaggta aagaggacca
     7501 gactagctaa aacgaaggga gcaaaaacac ctatggattt tcggatattt tgtccagcaa
     7561 gtttgaggta aaacatctac tcacccccta aaagtgcaga catattgagc gaaatctctt
     7621 tggcaaagtc ttgattgctc ttacctgcct tgtacaattg gtggaaaatc cgaccatcct
     7681 tgatgaagag gacgcgtttg gcatggctgg ctgcgttggc tgagtgagtt accatgagaa
     7741 ttgtttgtcc atctaggttg atgtcttcaa acaaattcag caagtcttcc gaattgcggt
     7801 aatctagggc agccgtcggt tcatccgcta gaacaatctg cggctgggta atcaaactac
     7861 gggcaatggc cacgcgctgc ttctgtccac cagacagctc aaaaggacgt tttttcagca
     7921 aatcttgaat ccgcagtttt ggcgccaaaa ctttcaaccg tttctccatt tcctcatagc
     7981 tctcacgcgc caataccaga ggtaaaaaga tattgtcttg gatagacagg gtgtccaaga
     8041 ggttaaaatc ttggaagaca aagcctagat tacgcaaacg gaaatctgcc agcttgcttt
     8101 ccttgatttt ggtgatgtct tgattattta agataacaga gcccctcgtc ggcttgtcca
     8161 gggttgctag aatgttgagc aaagtcgtct tacctgaacc actttcaccc atgatggcga
     8221 taaactcacc ctcttctacc ttaaaatcca catcttgcaa agcacgagtt tcctcttttg
     8281 aaaagcgcgt gcggtaaact ttttctaaat gattgatttc taataacata gttactccta
     8341 attccttatt cactgtctaa atcaacttgg tcaagaactg cttgatagtc gattcggtct
     8401 gattcagcaa tattatcttc taaatccacc ttataataac catcctggac accgatggcc
     8461 cattccttgc tagtggttat atagagatta ttcaaggata ctgtcttttt cactgccccc
     8521 tcttctttga caacaggcca ttccataaac ttgacttcta cttcttgaat attattctcg
     8581 gcaacttcct gtttgctaac ggcatagtct tttcccttgt aatggaaagt ttgatagccc
     8641 tcatcaaagc tcaggcctgc tgaagagaaa gaacaagcgg ttaggattat gctgcccaaa
     8701 gccagcaagc caaatacttt ctttttcata gtttcctccc tctagtatgc cataaatcgc
     8761 attgtttgca aatcaagtgc cagctcctct aaaaattctg cctgttccaa gagttcagtc
     8821 ttttcttttt ctgtgaatgc cttttcttcc attctcttta cttgtaaaaa tttaaatgac
     8881 atatgatttc cttctttctg tttcttatga gtctattata gaaaatatgt cgctcatgtt
     8941 ctcttacagt tctgattaaa aaacttacaa gagtgtaagt ttagagaaaa taaccaatcg
     9001 tggccagtat ccacaaatga agcggataga agatgtaaaa gaaatagcgt gaaaatactg
     9061 tgcggggccc tgcttgtcca ttataaagac taagaattgg aaggactaaa ataaatagcc
     9121 agtctgcatt gtagagcatc atctgaatgg tcatctcaag cctttcgtag ggctggtagg
     9181 acatggctaa taaaactaga gccagaaaac cataggcaat ggttcgctta gtagcagact
     9241 cacgacccaa ataggtgatg aggatgaatg gcaagaccac cgtgccccct tctgtgaaca
     9301 aggcacctat gattgtcaat aaccctgcca gcaggacctg aatgatttgc ttcgctctcg
     9361 tgtgctcact aaaattttga agacaaatca gcagagttag gccgactgct aaggtcaaaa
     9421 agatattatt gctaatgtaa atccctttag actggtagag actgtttagc aaagtatttc
     9481 cagtggccat tagtcctgct gcaccataca gtcgtaacag ataggctttg acatttctcg
     9541 tatgaagaaa cccttccacc aacatgtagg caaagaaaac cgcgacgcac cgtgttgcta
     9601 aatgaaacac taaagcgagt tgatcaggga taaaatcagg tatgattttc agatgatcca
     9661 aaaccatcaa gcctgccata gcgagtttca gttgataagc attatatgat ttcaccagtc
     9721 aaacctccta gtacatccag ttgcgttgga acagttcgtg ttctagctcg aatagaaatt
     9781 cagcttgttt gtcttgttct tctagtcgtt tgctttcttg tgatttacca aaaatttgtt
     9841 ttgcttgtag aaatttgaat gacatctgat ttccttcttt ctatttctta tgagtctatt
     9901 atagaaaata tgtcgctcat gttctcttac aattctgaac taaaaactta caggtttgta
     9961 agaaaagaaa aaagacacca ttttaggcat ctttcaattc attctatttt gttaaaccaa
    10021 tgtaggcaat catttcatcc aactctttct tgctttccgt tacaagtaga taggcatcat
    10081 ttccctgaag ttccgcaaag gcatctggca tgcgtttgac ggttttaatc ttaccagcga
    10141 atttctgatg aatgtcatct gctgagtgaa catattcggt cgtatcacgg cgagttgcga
    10201 ccaagcagta ttgtggttca aatctacgtt cctcaactgt accacctgtt gcgacattgg
    10261 cataatcacg gaaaagatca atggaatggg cgtagttata ggcttcaaca gtaaagccac
    10321 ccgccatgcg gttgttgtat tcaatggcaa tgtagtcgtc cccatccttg aaaaattcaa
    10381 tatggaaaaa acgctctctc ataccaaagg ccttgataat cgcgcgacca tattttacta
    10441 attgaggatc caactccttc tcaatgaagt aggcattgtc ttttctagac aacatcagtt
    10501 ccagcggagt atgaacataa gtcaaacctg tttcaaagac aacattgcct tcgtggtcca
    10561 acaaaccatc ataggttgtg ataatgcttg agttgacaaa tttttcgagg aaataaatag
    10621 ttgctccatc ccatgtctcc acaaaccgat caaaatcttc tttattttcg attttaaagg
    10681 taccggatgc cccaacaccg ttgtctggtt tagcaatcat tgggaaacca acattcttca
    10741 aagctctgcc aaaatcacgt tccgatttaa caaccacacc tggaacgact ggaacacctg
    10801 cttttttgaa atattttttc attttagact taaatttagt ctttttcagg tgtttgggtt
    10861 tggctccaaa aatgttgaat tgttcacgga gggctgcatc gttttcaagc caatattcat
    10921 tgtgggattc tacacggtca attggaccat gcttgtaaat aagaaaggcc gctgcacgct
    10981 tgacttcatc gatattttcc aaatcttcca cacgaaagta ttctgtaaga gcgttacgaa
    11041 gttcttcagg caattgatcg taaggttctt gcccaatacc tagaaccgta acgccttctt
    11101 ttttagccaa ttcaatggtg aacggttgaa aattttctgg ataaaaaggt gaaataacga
    11161 gataattcat aggtagtcct ttctaatctt aaagtccgat agaatgaagg aagaatggca
    11221 tttgctttct ccaccacacc cagtcgtggg ccacatctgt tccccaagta tcaaaccaag
    11281 ctgggatatt cttctcttca aaggcacgtt ttaggttgta gaatgaatcc agaccgtctt
    11341 gctcccaatc gcccaaacct gtgcagacga taatatctgc ttgacggtat ttgtcaataa
    11401 accaaccatc attctggttc cagatgtaat cagacggtga gttttgatag accgcatcat
    11461 catgaccata atcatggttg ttgttgaaga atcgagcatc atagacacct gaaagtgcaa
    11521 taactttcgt gaagacatct ggatgctgga ggaagaaatt gagcgcgtgg taggccccca
    11581 ttgagcaacc agttgtcatc ataccatcga accagccagt tttatgtttg ataaagggaa
    11641 tcgcttcttc aatcacatac cgttcatagg cgcggtgagc ctctgcctta tcatgaatgg
    11701 atttccagtc tgccaaccag ctttcactat cgtagctagt tagagtaaaa aattggacct
    11761 gaccagcttc aatagatcca cgacaagctt caatcatacc aaaatcagca tattcattgt
    11821 aagaaccacc tgatgaagca aatacgacaa caggaatccc tgcgtgacca taacgattta
    11881 gattcatttc tcggccaaga tttccagacc aatgacttaa aaattcaacg tgcatagtaa
    11941 agcctcctat ttgtatattt ttgttttatg accaatgttc gcttaggaat cgtaggcaag
    12001 ctggcaaata gagcgcccaa gcctcttcat tatggaccgc acccgaaacg acttccaagg
    12061 cgatattgtc caaatccact ccgccagcga tcaattgacg atagtaagtc aatgaagaat
    12121 tgatataggc ctgcttgata tttcctgcca tcaatgtttt atctgtatca tctgcttctt
    12181 ctgtcccaac gtagatgtag actcgttgct ctgagtctaa ttgttgacgt tcaatataac
    12241 ggtcgaaagc atcttgatga agccaattgg ccgatgaaaa gacacccaga cagccaatct
    12301 ggttctgata agccaagccc ataaattgtg tgatattgcc tcccaaggat gagccaatca
    12361 ttgcagtgtg aactttatca gattttgtcc ggtattcttt atcgataaag ggtttgacga
    12421 catccatcac aaattcagca tacaaggttc ccttaccacc gaactgcacc ccaggaatcc
    12481 ccatctcact aaacttccaa gcagcgtatt catgcatgcg atcagcacca tcgttatcaa
    12541 tagctactac gatcattttt gcaatgtctg gattacgctt aatagttggg atgactttcc
    12601 atgaatgacc tgaaaaagct tccttactgt agagtacatt ttgcccatcg tggaaataaa
    12661 caacgggata ggtctcttct gtattttcag catatccttt cggcaataaa acacggactc
    12721 gacgttttct tcctgtaaat ggtacatcta attcatgagt tctcatgtcc aaataaaagt
    12781 aagacttatt catatctatc tcttccttat gtatttta
//
LOCUS       DBNFEG010000044        12637 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.044, whole genome shotgun sequence.
ACCESSION   DBNFEG010000044 DBNFEG010000000
VERSION     DBNFEG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12637
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.044"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            51..1640
                     /locus_tag="AC1PNG_000871"
     CDS             51..1640
                     /locus_tag="AC1PNG_000871"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HIP6701384.1"
                     /translation="MSKTIQAITNQIWSMANELRGNMDASEYKNYILAFMFYRYLSEH
                     QERFLVEDEIISPAPGETVQDAYAREAVGNDLVEHLENTASHLGYAIEPEDTWARLVA
                     KIDNSEIVASDYQTIFDHFNANVELNRDAMEDFRGVFNDINLGDSRLGNSTVARAKSL
                     NSIVKLIDSIEYKNDEGKDILGEIYEYLIGQFAASAGKKGGEFYTPHQVSKILAKIVT
                     LGLEKSDTSFSVYDPTMGSGSLLLTVRNELPQGQHIKFYGQEMNTTTYNLARMNLMMH
                     QVSYSNMILNNADTLESDWPDGVDELGIDQPRSFDAVVANPPYSAKWDNRESKLKDPR
                     FMEYGKLAPASKADFAFILHSLYHLNNTGTMAIVLPHGVLFRGAAEGHIRKLIIEKNY
                     LDAVIGLPANLFYGTGIPTTILVFKKNRQTKDVFFIDASKEFEKGKNQNHLSDDMVEK
                     IVETYHNRQSVDKYAHLASIEEIVENDYNLNIPRYVDTFEEEEEIDLGQVTQQLEQDR
                     LEIRALEDKIRQQLKTLGVDF"
     gene            complement(1691..2965)
                     /gene="serS"
                     /locus_tag="AC1PNG_000872"
     CDS             complement(1691..2965)
                     /gene="serS"
                     /locus_tag="AC1PNG_000872"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIP6701385.1"
                     /translation="MLDIKRIRTDFDGVAAKLATRGVAAETLANIKELDAKRREILVT
                     VEELKAERNTVSAEIAQAKRNKENADDKIAAMQKLSADVKNLDAQLLEIDEELNAIIT
                     VLPNTPHDSVPVGADEEENVEVRRWGTPREFTFEPKAHWDLGEDLDILDWERGAKVTG
                     ARFLFYKNLGARLERALYNFMLDEHIAEGYQEIITPYMVNHDSMFGTGQYPKFKEDTF
                     ELADTNFVLIPTAEVPLTNYYRNEILEEKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFATPEQSYDELEKMTANAENILQKLGLPYRVLALCTGDMGFSAAKTY
                     DLEVWIPAQNAYREISSCSNTEDFQARRAQIRYRDAADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGGVEVIKP"
     gene            complement(3183..3554)
                     /locus_tag="AC1PNG_000873"
     CDS             complement(3183..3554)
                     /locus_tag="AC1PNG_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HIP6701386.1"
                     /translation="MAQSQNTSVELQTTGVSYMGFGGKVGKFLIGDKALEFYPDSNVE
                     RYIQIPWSEMTSIGANVSGKAISRHFEIYTEKSRFLFASKDSGKILKIAREHIGNEKV
                     VKLPTLMQTIGRKISNLFAKK"
     gene            complement(3614..4525)
                     /locus_tag="AC1PNG_000874"
     CDS             complement(3614..4525)
                     /locus_tag="AC1PNG_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIP6701387.1"
                     /translation="MSEKIQLSVSDRKKVWWRSTFLQGSWNYERMQNLGWAYAMIPAI
                     KKLYTKKEDQAAALERHLEFFNTHPYVASPILGVTLALEEERANGAEIDDTAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLALSGNIMGPIIFFLGWNIIRMAFLWYTQE
                     FGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVSITFAVDLPAKQLSEGA
                     YIVFPEGTVTGAELKGILGQALSGLSLEPTQAQTLQGQLDSLIPGLMGLLLTFLCMHL
                     LKKKVTPITMIIGLFIVGILARFFGIM"
     gene            complement(4543..5352)
                     /locus_tag="AC1PNG_000875"
     CDS             complement(4543..5352)
                     /locus_tag="AC1PNG_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HIP6701388.1"
                     /translation="MSDITLISAILVVIVAFFAGLEGILDEFQFHQPLVACTLIGLVT
                     GNLAAGVMLGGSLQMIALGWANIGAAVAPDAALASVAAAIIMIKGGDFSTEGIAVATT
                     TAIPLAVAGLFLTMIVRTISVGLVHGADNAAKDANFAAVERYHLFALLLQGLRIAVPA
                     ALLLAIPAEAVQAVLESMPAWLSGGMAVGGGMVVAVGYAMVINMMATREVWPFFALGF
                     AFAAISKLTLIALGVIGVAIALIYLNLSKQGGSGNGGASSNDPIGDILEDY"
     gene            complement(5384..6376)
                     /locus_tag="AC1PNG_000876"
     CDS             complement(5384..6376)
                     /locus_tag="AC1PNG_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIP6701389.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPSEGPDD
                     LYAKLNAAVDAFDADDEVLVLADLWSGSPFNQASRVAGENPDRKFAIITGLNLPMLIQ
                     AYTERMINAAAGVEEVAANIIKEAKDGIKVLPEELQPAEATPVADAAPVALQGAIPPG
                     TVIGDGKLKINLARIDTRLLHGQVATGWTPASKADRIIVVSDTVAKDELRKQLIKQAA
                     PGNVKANVVPIKKLIEVSKDPRFGNTHALILFETPQEALEAIEGGVEIKELNVGSMAH
                     STGKTMVNNVLSMDKDDVATFEKLRDLGVEFDVRKVPNDSKKDLFELINKANVQ"
     gene            complement(6598..7410)
                     /locus_tag="AC1PNG_000877"
     CDS             complement(6598..7410)
                     /locus_tag="AC1PNG_000877"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIP6701390.1"
                     /translation="MKKKIIAIDLDGTLLNNDSQLSDYTISTIKKVRQAGHTVLIATG
                     RPYRMAEKFYQQLELDTPMINFNGSLVHIPGKKWQWEQNILIDKKYLLEFLKEEETFE
                     ADFIAGEYKNKFYITQKNLDKIDPALMGVEQITPDTLIKPELITSDPHSILMQTRATD
                     KYELAKEMNAYFKDELEINTWGGPLNILETCAKGVNKATALSYVLNLYQATPSDLIAF
                     GDEHNDVEMLDLAGTAYAMKNCSDTLRPHADRLTAFANFEDGVARELENLFL"
     gene            7756..9215
                     /locus_tag="AC1PNG_000878"
                     /pseudo
     CDS             7756..9215
                     /locus_tag="AC1PNG_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
     gene            complement(9563..10423)
                     /gene="metF"
                     /locus_tag="AC1PNG_000879"
     CDS             complement(9563..10423)
                     /gene="metF"
                     /locus_tag="AC1PNG_000879"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HIP6701391.1"
                     /translation="MIGQTPSLSFEIFPPKPEVGNEKIIQTLDEMQGLAPHFISVTCS
                     NNNLNVEETTVKLANHVRNELHIPTIAHLPAAYLTKEKVRSVLHSLDEIGVHQILALR
                     GDIIEGLPPKEDFQYATDLISFIKEEAPHFDIIGACYPEVHPESPNSVSDIKNLKKKV
                     DAGCSTLVTQLFFDNEAFYNFQEKCSLADIEVPIIAGIMPIINRNQALRLLKTCENIR
                     LPRKFKAILEKYEHNPESLRAAGLAYAVDQIVDLVTNDVAGIHLYTMNNAETARTIHQ
                     ATHSLFKHYS"
     gene            complement(10427..>12637)
                     /gene="metE"
                     /locus_tag="AC1PNG_000880"
     CDS             complement(10427..>12637)
                     /gene="metE"
                     /locus_tag="AC1PNG_000880"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HIP6701392.1"
                     /translation="HRELKFITEKYFRNEIPQEELLEVAKDLRAKHWNIVKEAGITEI
                     PSNDFSHYDNVLDAAVLFNIVPKAVQNLDLTDLEKYFALARGYQGEKGDVRARPMKKW
                     FHTNYHYIVPAIEKDTEIKLAGHKIFDEFQEAKNLGITTRPVLIGPFTLLQLTDFEEG
                     LTATDFADSLVAAYGQVFEKLAELGAEKIQLDEPSLVKDLTAEEKALFLRIYQTLLAD
                     KKGLQVLIQTYFGDVRDIYTELTSLPVDAIGLDFVEGKETAALVATGFPANKTLYAGI
                     VNGKNIWRNNYEKSLAVLDAIPAEHVVITTSCSLLHVPFTTANEEFEPAILNHFAFAV
                     EKLSELRDLDAIRNSQGEVALTANKELFALERVGRDAALADRLAGLTDADYTRLPVFA
                     EREAIQREKLNLPLLPTTTIGSFPQTKEVRSTRLAFRKGNISEEEYDTFVKAQTDEWI
                     AWQEEVDFDVLVHGEFERNDMVEYFGENLSGYLFSKNGWVQSYGMRGVKPPIIWGDVT
                     RLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKESTLQIALAIKEEV
                     LDLEAAGIKIIQIDEAALREKLPLRRSDWYSEYLDWAIPAFRLVHSTVAPETQIHTHM
                     CYSEFTDIIPAIDNMDADVISFEASRSNLVILDELKAKNFQTQVGPGVYDIHSPRVPA
                     VDEIAHTIQAILAKVPKEKVWINPDCGLKTRGESETKASLIHLTQAAKTARKEL"
BASE COUNT         3527 a         2512 c         2770 g         3828 t
ORIGIN      
        1 aaatgagatt tcgagtaaaa aatgaaacaa catgaaagga ttacaaaaac atgtcaaaaa
       61 ctattcaggc cattactaat cagatttggt ccatggccaa tgaattgcga ggaaatatgg
      121 atgcttcgga atataagaac tatatcctag ccttcatgtt ctatcgctat ctatctgagc
      181 atcaagagcg tttcttggtg gaagatgaaa ttatcagtcc tgctccagga gaaactgttc
      241 aggatgccta tgcgcgagaa gcagttggga atgacttggt agagcatttg gaaaatacag
      301 cttctcacct tgggtatgcc atcgagcctg aagatacctg ggcaagactg gtagccaaaa
      361 ttgataacag tgagattgta gcgagtgact accagacgat tttcgatcat tttaacgcca
      421 atgtagaact caatcgggat gctatggaag atttcagagg cgtctttaat gacatcaatc
      481 taggggactc tcgtttgggc aattcaacgg ttgcgcgtgc aaaatccttg aacagtattg
      541 tgaagttgat tgatagcatt gagtataaaa atgacgaagg aaaggatatt ctgggagaaa
      601 tctatgagta tcttattgga cagtttgccg cttcggcagg taaaaaaggt ggagaatttt
      661 ataccccgca tcaagtgagt aagattttag ccaagattgt tactttaggt ttggaaaaat
      721 cagatactag cttctctgtt tacgatccaa ccatgggttc agggtctttg ctactaacag
      781 tgcgtaatga actacctcag ggccagcata tcaaattcta tggccaagaa atgaacacaa
      841 ctacttataa cttggcgcgg atgaacctga tgatgcatca ggtcagctac agcaatatga
      901 ttttgaataa tgctgatacc ttagaaagtg actggccgga cggggtcgat gaacttggaa
      961 tcgatcagcc acgtagtttt gatgcggtcg tcgcaaaccc accctactct gctaaatggg
     1021 acaatcgtga gtccaaattg aaggatccac ggtttatgga atacggtaag ctagcaccag
     1081 cctctaaagc ggactttgct tttatcttac acagtcttta tcatctgaat aatacaggga
     1141 ccatggccat tgttttacca cacggagtgc tctttagggg agcagcagaa ggtcatattc
     1201 gcaaactgat tattgagaaa aattacctgg atgcagttat tggcttgcca gctaacctgt
     1261 tttatggcac aggcattcca accactattc tggtcttcaa aaaaaatcgt cagaccaagg
     1321 atgtcttctt tatcgatgcg agcaaggagt ttgaaaaagg gaagaaccag aatcaccttt
     1381 ccgatgatat ggtagaaaaa atagtagaga cctatcacaa tcgccagtct gttgacaaat
     1441 atgctcattt ggcatcaata gaagaaattg tagaaaacga ctacaatcta aatattcctc
     1501 gttacgtcga tacttttgag gaagaggaag agattgatct cgggcaagta acgcagcaac
     1561 tagaacaaga tagactagag attcgggctc tagaagataa gattcgtcaa caattaaaga
     1621 ctttgggagt agatttttag ataaatgaaa agagcgagta ggatttttcc tcgctctttc
     1681 tttgtatgtc ttaaggttta atcacttcta ccccacccat gtatggacgg agaacttctg
     1741 gaatggtgac agaaccgtct tcattttgat agttttcaag gattgccgca acggtacgac
     1801 caactgctag accagaaccg ttgagggtgt ggagcaattt gaccttgccg tcagcagcat
     1861 cgcggtagcg gatttgggca cgacgagctt ggaaatcctc ggtatttgaa cagcttgaga
     1921 tttcacggta ggcattttgg gcaggaatcc agacttccaa gtcgtaggtc ttagcagctg
     1981 agaagcccat atcacctgta cagagggcca aaacacggta aggcaagcct agtttttgaa
     2041 ggatattttc tgcgtttgcg gtcatttttt ccaattcatc ataagactgt tctggtgtgg
     2101 caaatttgac catttcaacc ttgtggaatt ggtgaaggcg gataagacca cgggtgtcac
     2161 gaccagcaga accagcttca gaacggaaag atgggctcat ggctgtgaag tagattggca
     2221 ggtctttttc ttccagaatc tcgttgcggt agtagttagt caacggcact tccgcagttg
     2281 ggatgaggac gaagttagtg tctgccaact caaaagtatc ttccttgaac ttaggatatt
     2341 gaccagtacc gaacatagag tcgtggttga ccatgtaagg tgtgatgatt tcttggtagc
     2401 cttctgcgat atgctcgtcc aacatgaagt tgtagagggc acgttctaag cgagcaccca
     2461 agttcttgta gaaaaggaag cgtgcaccag ttacttttgc gccacgttcc cagtccaaaa
     2521 tatccaggtc ttcgcccaaa tcccagtgag cttttggctc gaaagtgaat tcacgaggtg
     2581 ttccccaacg gcgaacctct acgttttctt cttcatccgc accaactgga acagaatcgt
     2641 gcggcgtatt tggaagaacg gtgatgatgg cgtttaattc ttcatcgatt tccaaaagct
     2701 gagcgtctaa gtttttcaca tccgcagaca gtttctgcat ggcagcaatc ttgtcgtcag
     2761 cgttttcctt attgcgtttt gcttgggcaa tctccgcaga aacggtgttg cgctctgctt
     2821 ttaattcttc tacagtaacc aagatttcac ggcgttttgc atccaattcc ttgatgtttg
     2881 ccaaggtttc agcagccaca ccacgagtag ctaatttggc agccactccg tcaaaatcag
     2941 tacgaatacg tttgatatct agcatagatg tctcctttag aataaaaaag cacagaatga
     3001 gagatgctgg agcgatattt accgcggttc catccagctt cacattctgt gcacttgatt
     3061 gtattttgat aatttcataa cggtagaatt tcacaacttt ttcctatctg ctcacagcga
     3121 ccgcagactt tctggaagga gccagatgct acttatccgt ttctactatt atacttgaat
     3181 ttttattttt tggcaaatag attcgaaatt tttctgccga ttgtttgcat aagagtcggt
     3241 aatttcacaa ctttttcatt gccgatatgc tcacgggcaa ttttaagaat cttaccagaa
     3301 tctttagatg caaacaagaa tcgacttttc tctgtataaa tttcaaaatg acggctgatt
     3361 gctttgccag aaacgtttgc gccaatgcta gtcatttctg accaaggaat ctggatatag
     3421 cgttcaacgt tgctatctgg gtagaattca agtgccttgt caccaattag aaatttacca
     3481 accttgccac caaagcccat gtaggaaacc cctgtggttt gtagttcaac gcttgtattt
     3541 tgtgattgtg ccatagtgct ctctccctag atattcatta tatcataaaa ccccgcaaga
     3601 gcggggtcct tgattacata attccgaaga aacgagcaag aatacctacg atgaagagac
     3661 cgataatcat tgtgattggt gttactttct tcttaagcaa gtgcatgcag aggaaagtaa
     3721 ggagaagtcc catcaaacca ggaatcaagc tgtccaactg accttgtaat gtttgagctt
     3781 gagttggttc aaggctaagt ccgctaagag cttgaccaag aatacctttc aactcagcac
     3841 cagtaacagt accttctggg aagacgatgt aagcaccttc tgacaattgt ttagcaggaa
     3901 gatctacagc gaaagtaata gatacccaac gttgaacaag aacggcaagg atgaacatac
     3961 caaggattga agcaccttta gtgatgtctt gaaggatacc accagacatg tctttagtga
     4021 tttcgctacc agctttgtaa ccaaattctt gtgtatacca taggaaggcc atacgaatga
     4081 tgttccagcc aaggaagaag atgattggtc ccatgatatt tccagatagt gcaagtgaag
     4141 caccaagagc accaaggatt ggacgaacag taaaccagaa gactgggtca ccgataccag
     4201 caagaggtcc catcataccg attttcacac cttggatagc tgtgtcatcg atttcagcac
     4261 cattagcacg ctcttcttca agggcaagag ttacaccaag gattggtgaa gcaacgtatg
     4321 ggtgtgtgtt gaagaattcc aagtggcgct caagagcagc agcttgatct tcttttttag
     4381 tgtaaagttt tttgatagct gggatcattg cataagccca acctaagttt tgcatacgct
     4441 catagttcca tgaaccttga aggaaggttg aacgccacca aactttttta cgatcgctta
     4501 cggaaagttg aattttttct gacatgatga atctcccttc tcttagtagt cttcaaggat
     4561 gtcgccgatt gggtcgttag atgaagcacc accgttacct gatccgcctt gttttgacaa
     4621 gttgaggtag ataagagcga ttgcaacacc gataacacca agtgcgataa gtgtcaattt
     4681 gctgatagca gcaaatgcaa aaccaagtgc gaagaatggc catacttcac gagttgccat
     4741 catgttgata accatagcat aacctacggc aacaaccata ccaccaccaa cggccatacc
     4801 acctgacaac caagctggca tagattcaag aactgcttga acagcttctg caggaattgc
     4861 aagaagaaga gccgcaggaa ctgcgatacg caaaccttga aggagaagag caaacaagtg
     4921 gtaacgttct acagcagcga agtttgcatc tttagcagca ttgtcagcac cgtgtacaag
     4981 accgactgag atagtacgaa cgatcattgt aaggaaaaga ccagctacgg caagagggat
     5041 agcagttgtt gttgcaacgg caataccttc agttgagaag tcgccacctt tgatcatgat
     5101 gattgcagca gctacagaag caagagcagc gtcaggtgca acagctgcac caatgtttgc
     5161 ccaaccaagt gcaatcattt gtagagaacc accaagcata acacctgcag ccaagttacc
     5221 agtcacaaga ccgataaggg tacaagctac caatggttga tggaattgga attcgtcaag
     5281 gataccttca agaccagcga agaaggcaac gatgacaacg agaattgcag aaataagtgt
     5341 aatatctgac atgttgtaat cctttctata ctaacagatc gatttattga acgtttgcct
     5401 tgttgataag ttcaaacaag tctttcttcg aatcgtttgg tactttacgc acatcaaatt
     5461 caacgccaag gtcacgcaat ttttcaaagg ttgcaacgtc atctttgtcc attgacaata
     5521 cgttgttaac cattgtttta ccagttgagt gagccataga accaacgttc aattctttga
     5581 tttcaacacc accttcaatt gcttcaagag cttcttgagg agtttcgaaa agaatcaagg
     5641 cgtgagtgtt accgaatcga gggtctttag aaacctcaat caatttcttg attgggacaa
     5701 cgttcgcttt tacgttacca ggtgcagctt gtttgatgag ttgtttacgc aattcatctt
     5761 tggcaactgt atctgataca acgatgatac gatctgcttt ggaagcagga gtccaaccag
     5821 tcgccacttg accgtgcaag agacgagtgt cgatacgggc taagttgatt ttcaacttgc
     5881 catcaccgat aacagtacct ggagggatag caccttgtag tgcaacagga gcagcatcgg
     5941 caacaggagt agcttcagct ggttgaagtt cttctggaag aactttgata ccatctttgg
     6001 cttccttgat aatgtttgcc gcaacttctt ccacgccagc agcagcgtta atcatacgct
     6061 ctgtgtaagc ttggataagc attggcaagt ttagaccggt gatgatagca aacttacgat
     6121 ctggattttc accagctaca cggcttgctt ggttgaatgg tgaaccactc caaaggtcag
     6181 ccaaaaccaa aacttcatca tcggcatcga atgcgtccac ggcagcattt agcttagcgt
     6241 ataaatcatc tggaccttca cttggcataa aagtaacaac ttgtactttt tcttgttcgc
     6301 caaagatcat tgaacctgac tgatgaatac cagcagcaaa ttcgccatga cttgcaataa
     6361 tgattccaat actcattatt gatcctccta aatttttctt gttcatgtaa aaaccattga
     6421 atgggctttc atgttcgaac tataccatta taaaacgttt tcagtacatt tgtcaagtag
     6481 gtttgtgtta aaagtgcaaa atatttccaa aacttttcat aatcatgttt cagaaaagtg
     6541 ataaaaatat gcaacgaata tgacaaaaac ctgacagttt atcaggtttt ttgatgtcta
     6601 taaaaatagg ttttctagtt cacgtgcgac accatcttca aaattagcaa atgctgtcaa
     6661 tctgtcggca tgtggacgaa gtgtatcgct acaatttttc atggcatagg ctgttcctgc
     6721 taggtctagc atctcaacat cgttgtgctc atcgccgaag gcaattaagt cgctaggagt
     6781 ggcttggtag aggttaagaa catagctcag agcggttgcc ttattgacac cttttgcaca
     6841 agtttcgagg atatttaatg gtccgcccca cgtattgatt tctaactcat ctttgaaata
     6901 ggcattcatt tcttttgcaa gttcatactt atcagttgca cgagtctgca taaggatgga
     6961 atgaggatca ctagtgatta attcaggttt gatgagtgta tctggagtaa tctgttcaac
     7021 ccccatcaga gccggatcaa tcttatctaa gttcttttga gtgatgtaga atttattctt
     7081 gtattcacca gcaataaaat ctgcctcgaa tgtttcttct tcttttagaa attcgagtag
     7141 gtattttttg tcaattaaaa tattttgttc ccattgccat ttctttccag ggatgtgaac
     7201 taaagaaccg ttgaaattaa tcatgggagt atccagttct agttgttggt aaaatttttc
     7261 agccattcga taggggcgac ctgtggcgat aagaacggtg tgtcctgctt ggcggacctt
     7321 ctttatagtt gagatggtat aatcagataa ttggctatca ttattgagca atgtgccgtc
     7381 caaatcgata gctatgattt ttttcttcat atttaatagc attcctttct agagttgtta
     7441 tactagtttt taatagtaaa taatgtttag ctagtagcta gattgctgag aaattgtttt
     7501 atgcgtcaaa gaatagtact tgtaggtaac catatcttga actaataact tccagtataa
     7561 caaaaggaga taataaaagc aaagaagcga taaattttgt atagattttt gtaagtctgc
     7621 aacgttcggg atttgcgaat gtttaaagtt gaaaatcaat aaaataacgc tttcaagatt
     7681 aaaaaacttg aaattataat gttttttgat tataatgttt ttattgttcg taaattaaaa
     7741 ggagaaatga aaaaaatgga tcaatttttt aaattgaaag aacatggaac gactgtttct
     7801 acggaaatta tggcaggcct tacgactttc tttgccatgt catatatctt gtttgttaac
     7861 cctagtgtgt taagtgttgc tggaatgcca gcgcaggctg ttttcttagc gactattatt
     7921 gccagtgcag tttcaacgct tgttatggga ttatttgcta acgtacctta cgccttggca
     7981 caggtatggg gttgaatgcc ttctttactt atacagtggt tattggtctt ggttttactt
     8041 ggcaagaagc tttggcaatg gttttcctct gcggtttgtt taacgttttt atcacagtta
     8101 caaaggttcg taagagtatt atcaaggcga ttccagttag tttacaacat gccatcggtg
     8161 gaggtatcgg ggttttcgtt gcctatcttg gttttaaaaa ctctaattta atcaccttct
     8221 tgacatctgg ttcagatatt attactgtaa atggtgttgc tccggcagat gcaacagccg
     8281 aaacattctc aaacggtgtt ttctcagttt ttgctggtgg tggtgtcgta cctggtattt
     8341 caacctttac tgatccaagt gttctcttga cagtgttcgg tcttcttttg acagctgttc
     8401 tggttattaa gaacgtgcgt ggtgcaattt tgattggtat tattgcaacg actttagctg
     8461 gaattccagc aggtgttgtg gacctgtcta ccatcgattt tgcaaataac aatatcggta
     8521 cagcctttgc ggagcttggt acaactttct tagcggcttt tgatggtcta tcttcgctct
     8581 ttgctgactc aagccgtcta ccacttgttt tgatgactat ctttgcattc agcttgtcag
     8641 atacctttga tacacttggt acctttatcg gtacaggtcg taagactggt attttctcag
     8701 aagaagatga gaaagctctc gaaaatggta caggctttaa ctctaagatg gacaaggcgc
     8761 tctttgcaga tgcgattggt acctctatcg gtgcactatt tggtacttca aatacaacga
     8821 catatgttga atcggctgca ggtatctctg caggtggtcg tactggtctg acagctgtta
     8881 caactgccgt tctcttcttg ctatcaattt tgattttacc attcattgga attgtgccaa
     8941 atgctgcaac agctccagcc ttgattatcg tcggtgtcat gatggtttca tcctttcttg
     9001 atgttgattg gagccagttt gaagacgctc ttccagcctt ctttgccgcc ttcttcatgg
     9061 cgctttgctt ctctatctca tacggtattg cagccgcatt tatcttctat tgcttagtaa
     9121 aagtttcaac tggtaaggct aaagaaattc atccaatcct ttggggtgcc acagctctat
     9181 ttattctgaa tttcatcatt ctagccatct tgtaatctgt ttattatcct tgaactgatc
     9241 ttcatttgaa ggtcagtttt tttatttatg attaaaatat atgggaatca atgtgattta
     9301 ttcagcatat gcggtattga acgttgtaat ttgaaaacgg atggagttta tttttccatt
     9361 tcgttgcttc tctgtctcct cttagtgcca tttttactcc ttgtgactgt attttaaatg
     9421 gctgttcgtt attgtaagca agatgtctca tacctagaca tccgctggat taatgtcatg
     9481 atggggtgaa aaaattatca tgtagtcttt gggagtcaca gggtatagaa taacccctga
     9541 gcttgggttc agggggagtt ttctaactat aatgtttgaa taacgaatga gttgcttgat
     9601 ggatggtgcg ggccgtttcg gcattgttca tagtgtagag atggatacca gccacatcat
     9661 tggtcactaa gtcaacaatt tgatctacag cataggccag acctgctgca cgaagtgact
     9721 caggattgtg ctcatatttt tccaggatag ctttaaattt acgtggtaag cgaatgttct
     9781 cgcaggtttt caagagacga agtgcctgat tcctgttgat gataggcata atacctgcga
     9841 taataggcac ttcgatgtct gctagagaac atttctcctg aaagttataa aatgcttcgt
     9901 tatcgaagaa gagttgagtt accaaggttg agcaaccggc atcaactttc tttttcaaat
     9961 ttttgatgtc tgagacagaa tttggtgatt ctggatgtac ttctggataa caggctccaa
    10021 taatgtcaaa atgtggggct tcttccttga taaaggaaat caagtcagtt gcatactgga
    10081 agtcctcttt agggggaaga ccttcaataa tatcgccacg aagtgctaaa atctgatgta
    10141 caccaatttc atcgagagaa tgaagaacgg atcggacctt ttcttttgtc aagtaagcag
    10201 ctggcaaatg tgcgatagtt ggaatatgca attcattgcg aacatggtta gccaacttga
    10261 cagtagtctc ttctacgttc aaattgttat tgctgcatgt aacactgata aaatgagggg
    10321 ccaaaccttg catctcatcg agagtttgga taatcttttc attgcctact tctggttttg
    10381 gaggaaaaat ttcaaatgag agacttgggg tttgaccaat cataaattag agttcctttc
    10441 tggctgtctt agctgcttga gtcaagtgga taagactagc ttttgtttct gattcgccac
    10501 gagtcttgag tccgcagtct gggttaatcc aaactttttc tttcggtact ttagcaagga
    10561 tggcttgaat ggtgtgtgca atttcatcaa cggcagggac acgtggagag tggatgtcat
    10621 agacaccagg acctacctga gtttggaagt ttttagcctt gagttcatct agaataacaa
    10681 gatttgaacg gctggcttca aaggagataa cgtctgcatc catattgtcg atggcaggaa
    10741 tgatgtcagt aaattcgcta tagcacatgt gagtgtgaat ttgtgtctct ggtgcaacag
    10801 ttgagtgaac taatcggaag gctggaatcg cccaatccaa atattcactg taccagtcgc
    10861 tacgacgaag tggtaatttt tcacgtaaag ctgcttcgtc aatttggata atcttgatac
    10921 ccgctgcttc aaggtcaaga acttcttcct tgatagcaag ggcgatttga agagttgatt
    10981 ctttaataga aatatcttca cgagggaatg accagttgag aatggttact ggccctgtca
    11041 acataccttt aactggtttg tttgtacggc tttgtgcata gcttgaccat ttaacagtga
    11101 ttgggttcaa gcgcgtcaca tccccccaga tgattggtgg ttttacccca cgcataccat
    11161 atgattggac ccatccgttc ttactgaaga gataaccaga aaggttttca ccgaagtatt
    11221 ctaccatgtc gtttcgttca aactcaccat gaaccaagac atcgaagtca acttcttcct
    11281 gccatgcaat ccattcgtct gtttgagctt taacaaatgt atcgtattct tcttctgaga
    11341 tatttccttt gcggaaagcc aagcgtgtgc tacggacttc cttggtctga gggaaggaac
    11401 cgatagtggt tgttggaaga agtggtaagt ttagcttctc gcgttgaatg gcttcacgct
    11461 ctgcaaagac tggtaggcgt gtataatcgg cgtctgtcag tcctgcaaga cggtctgcaa
    11521 gggctgcatc acgaccaaca cgctcaaggg caaagagttc cttgttagct gtgagagcaa
    11581 cttcgccttg actgttacgg atggcatcca aatcacgcaa ttcgctcagt ttttcaactg
    11641 caaaagcaaa gtggttgagg atagctggtt caaattcttc atttgctgtt gtgaaaggta
    11701 catgaagaag ggagcaagaa gttgttataa cgacatgttc agctggaata gcatccaaaa
    11761 cagccaggct cttttcgtag ttgttgcgcc agatattttt accgttgacg ataccagcgt
    11821 agagggtctt gttagctggg aaaccagttg caacaagtgc agcagtttcc ttaccttcta
    11881 caaagtcaag accgatggca tcaactggta ggctggtcaa ttctgtgtaa atatcacgaa
    11941 catcaccgaa gtaggtttgg atgagaactt gcaaaccttt cttatctgcc aagagtgttt
    12001 ggtagatacg taggaaaagg gctttttctt cagcagtcaa atccttgacc aagctcggtt
    12061 cgtccagctg gattttttca gcaccaagct ctgccaattt ctcaaagact tgtccgtagg
    12121 ctgcaactaa gctatcagcg aaatcagtag cagttaaacc ttcttcaaaa tcagttaatt
    12181 gtaagagtgt gaatggacca atcaaaactg gacgagttgt gatgcccaag ttctttgctt
    12241 cttggaattc atcgaaaatc ttatgaccgg ctagtttgat ttctgtatct ttttcaatag
    12301 ctggaacgat atagtggtag ttggtgtgga accatttttt catcggtcta gcacgaacgt
    12361 cccctttttc tccttgataa ccacgtgcaa gagcgaagta tttttcaagg tcagtcaagt
    12421 caaggttctg tactgcttta ggtacgatgt tgaagagaac agctgcatcc aaaacattgt
    12481 catagtggga gaagtcgttg cttgggattt cagtaatgcc tgcttcttta acaatattcc
    12541 agtgcttagc acgtaggtct ttagctactt ccaaaagctc ttcttgtgga atttcatttc
    12601 taaagtattt ttcagtgata aattttaact cacggtg
//
LOCUS       DBNFEG010000045        12600 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.045, whole genome shotgun sequence.
ACCESSION   DBNFEG010000045 DBNFEG010000000
VERSION     DBNFEG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12600
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.045"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            148..408
                     /locus_tag="AC1PNG_000881"
     CDS             148..408
                     /locus_tag="AC1PNG_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HIP6701393.1"
                     /translation="MDVKNLRKLKRIELYEIMLAQSEEIDCLRNQLEKIKTELADRRI
                     MISQSGSIAEASMKLTNIFEEAQKAADLYLYNVKNKIGDVHE"
     gene            401..991
                     /gene="lepB"
                     /locus_tag="AC1PNG_000882"
     CDS             401..991
                     /gene="lepB"
                     /locus_tag="AC1PNG_000882"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIP6701394.1"
                     /translation="MNSSNLRGKKLPKTSDLQAVYQQAKYKKTFWETIRSTVFMLVVV
                     AAFAVLIAVLFLPILRIYGNSMKGTLNSGDIVVSVKSNDFESSDVVAFYYNNNILVKH
                     VIAEAGDWVDMDKQGNVYVNNQRLDEPYLANRDYSHTDIEFPYQVPENRIFVMGDNRK
                     ESIDSRNNAIGTVSNEQIVGKLVFKIWPLPELGWIE"
     gene            1023..1622
                     /locus_tag="AC1PNG_000883"
     CDS             1023..1622
                     /locus_tag="AC1PNG_000883"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701395.1"
                     /translation="MRDWLNLEKIQGWRRKYKKLVNIIAFLVVFATTYALILPAITLD
                     QEKAEQTAGIEVQETPVTTEISKLTEPMVTTQQTPIVGSSADEVQSPATEAVPDPAEQ
                     VSTESASKPQTDQERITEPTTIVHKTNDYEVTATFDASAQFPKDVEFNVRELDSQSEE
                     YRTHYEKTKEKLGVKNLYMPDSLIFIFHHRAKRSSLLPL"
     gene            1868..6010
                     /locus_tag="AC1PNG_000884"
     CDS             1868..6010
                     /locus_tag="AC1PNG_000884"
                     /inference="COORDINATES: protein motif:HMM:NF037639.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIP6701396.1"
                     /translation="MIDKTVGTSLGSLPEAPFKNGYVFSHWINKDTGDRIDENTVITG
                     DLTAVAVFNNINIYTIDVTYFYHNVSKNQDITIDKETVQLEASDAPYQLTPPASTEVS
                     NKEDTTLPADAIYYPEKPLLEFTADQLERLDVADGVDDNHISERVQYVPYNAEYDVIY
                     RLKNLTGSGYSDIQTVHVRGVLGSTVTPQILTYNYADFERADTAKIENTSGQKLYVYY
                     TRKTFTLSYNSNGGSNIPQQQGLYDSQIKISTTTPTKEGYTFEGWYDNPGLTGNKITG
                     SLTLKKDTTLYAKWTPNRVRYIIGYYKEVYDNNTGTTSYVYDSSAAGWGLVGTTVKGT
                     DSNIGPPPPTQTTIGSIGYEKDVTKSAASSIEIAADGTSVLKVYYKLKRYTFEFFLND
                     NNANITINGKTYSDRNRYMIRDVVLGQDVSKLWPASITNPKEVYDDKVYYDSYGRPIR
                     YFFDYWIFGNLLFKTDRFEVVEPMLPTSGTTSKFTAYWNTQGTQANVEYWLQQPDGSY
                     KKSDIYSQSFLKTAGLLPKEIFGYSYYTDASFTEAGIRYYGTQGNTYRFYYKRQAFKI
                     DYYYNSTGLDSKSNILFESDINTATYNYQPPRSTGVDADYTWGGWYTDSLLTEKYNFN
                     KMPANNLVLYAKWIAPKFKVTFDINGGNGTAPVDQSVEKYKYAKAPADPTREHYNFLG
                     WSTEDGKLYSWSDQVTKDVKLTAKWELKPLTYTVRYLEAGTNTQLAADKVITSPALVL
                     NQAITEKALGITGYRPDKSSQTINLNFRGNEITFYYTPKVKDVVYTIKYVLKDNPAIE
                     VAPTVTRKVDGSIIRAKEAAADVNKQHLQSQSGVTAEMLEMDYYPTVNTQSLILTSND
                     ANNVIVFEYVGFDTQTFTVNYLDMDGKKIDGQEPLVVYRKKPGSFVVDHKTINGYTYD
                     HSLDSENAADKTVYRVSKGGHITIDLYYKKNLTLTALDKSKVYDGTALSSSGLSDLNP
                     AYSVDLKAGDKLVAIGYDGSQTNAGSSATTPKLANISNALGADRTNFYHITYQAGNLR
                     VDQQPVVVVINGEKKSKIYDGKAEAVGYKVMEIRDTSGLYKETDIHFNGSSSEQSVKK
                     KDAGIYPLLLNNRFTNTNQNFQVDFLISDGELRIDRRQLTITSDSASKDYDGSELRAD
                     NITVSVPAGVDYTGFVDGEGVVPTFLGGPTEPGFMPNYFTYQPKDGTNLANYELETQF
                     GQLKVKEVIKIQKTDLAWQALSGGKFELTKWDGLNWAQVDGAQEFAITSKDGSNIPVG
                     LEPGRYRLQELAAPDGFIILDSYIYFTIKDNFNEDQTMSFYTVTLSDEMGNDATPERA
                     QLERTTGDASHRIKVANEQGRALPSTGGSGQKWFILSGLVLMVTSYLIHLFVQRNRKM
                     RS"
     gene            6108..7544
                     /locus_tag="AC1PNG_000885"
     CDS             6108..7544
                     /locus_tag="AC1PNG_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HIP6701397.1"
                     /translation="MNKFKSLFMTVLAVLAMVLPTTVASAQEVDTNAGGTGTITVRNA
                     SQSQKYTIYKLFDATVTEDGSGISYKVPTGKILPADNPYFAVDSKGNITAKEGATVSS
                     EAFAAWAKNFGTEVTSATANDNSLVFTHLTFGYYFVTSSLGAVLTVDSTTPNATVIDK
                     NTTNPTIPDSNNGGGKKILSNGATTSETTAKIGDTINFQIKFNATNYVTKDRQTKQIV
                     SYAIEDSPTALAIDQNSVNVKVDGVDITAKISKTFDATGKMNLVLTWADAAASNKTIY
                     NSPAEVIITYSAVVTKDAKEGEATNSATIGYNTIDNPTTPPTPVDPDKPTETTKVTTH
                     RFTLKKTNNVGETLTGAEFKLYDAANNGTEIKVVKESDGVYRVAQADEQGVVIEAGEV
                     VIKGLKHSTTYYLEEMKAPNGYNILTERQSIEVKENNTAQANIVNKKGGVLPSTGAIG
                     ATLFYLVGSILLLVALVYTISKRRMNNI"
     gene            7658..8527
                     /locus_tag="AC1PNG_000886"
     CDS             7658..8527
                     /locus_tag="AC1PNG_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIP6701398.1"
                     /translation="MIKWLKKKGPSFIMPLIFILGIGLLAYPSVSDYWNSFHQSEAIM
                     SYSDTVSDMTADQYKEIIDSARQYNQEKPLNWNVTASDIEAYNQELNFNKDGIMGYIE
                     IPKIDVKLSIFHGTDETVLETSIGHLEGTSLPVGGLGTHSVLSGHRGLPSARLFSDLD
                     KLREGDIFTIHVLNETLTYKVDQIRVVEPSDLSALTIEPQKDLLTLVTCTPYGINTHR
                     LLVRGYRIENVNGSALVTSDAIQIKAIFIAPFIATPILFVILIYIFITTSKTFRSWNR
                     NQVLDDFLVPKKF"
     gene            complement(8641..8918)
                     /locus_tag="AC1PNG_000887"
                     /pseudo
     CDS             complement(8641..8918)
                     /locus_tag="AC1PNG_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018958.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            9089..10438
                     /gene="murD"
                     /locus_tag="AC1PNG_000888"
     CDS             9089..10438
                     /gene="murD"
                     /locus_tag="AC1PNG_000888"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIP6701399.1"
                     /translation="MKMIDIVKNKKVLVLGLAKSGESAARLLDKLSAIVTVNDGKPFE
                     ENPTAQSLLEDGIRVICGSHPLELLDEDFALMVKNPGIRYDNPMVEKAIGKGIPVWTE
                     VELAYLVSDAPIVGITGSNGKTTTTTMIAEVLNAGNKPAKLCGNIGYPASSVAQTATA
                     EDTLVMELSSFQLMGTESFRPHIAVVTNLIASHIDYHGTFEDYVAAKWMIQGQMAAED
                     FVVLNFNQKEAKELAEQTKATVVPLSTLEVVDGAYLADGKLYFKGEYIMDADQIGVPG
                     SHNVENALATIVVAKLLGVDRQAIQESLSAFGGVKHRLQFVGEINGVSFYNDSKSTNI
                     LATQKALSGFDNSKVILIAGGLDRGNEFDELVPDLVGLKKMVILGQSAPRVQRAADKA
                     EVETIEALDIADATRKAFAIAEKGDIVLLSPANASWDMYANFEVRGDVFLQTFEELK"
     gene            10441..11505
                     /locus_tag="AC1PNG_000889"
     CDS             10441..11505
                     /locus_tag="AC1PNG_000889"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIP6701400.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLIPRFLEEGWEVHYIGDGNGIEHE
                     QVVKSGLDVHFHSISTGKLRRYFSFQNMLDVFKVGFGVLQSVAIIAKIRPQALFSKGG
                     FVSVPPVIAANLLRVPVFIHESDLTMGLANKIAYKFATTMYSTFEQPASLTKVKHVGA
                     VTKVGKTNDKVTPIQLPEILSHFDKSLPTLLFVGGSGGAKVFNDLISQNSAALTERFN
                     IINLTGDSSLNQLDKNLYRVDYVTELYQPLMDLADVVITRGGSNTLFELIAMQQLHLI
                     VPLGRQASRGDQIENALYAEKKGYSKQIDESQLTFASLLVEVDELLKNKEFYIQNMTN
                     SNEIQSVDSFYNLLREDMGR"
     gene            11511..>12600
                     /locus_tag="AC1PNG_000890"
     CDS             11511..>12600
                     /locus_tag="AC1PNG_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIP6701401.1"
                     /translation="MTEKDSNVEESVLEVEQASQVELDSEQISPAEKESVLAEEKGLS
                     TDVSTDVDIPEMTAPDDEKSAFFEQWKARHQAYLAHKDAAEIQAVDEGQTEQENPEAK
                     KSKRVLFQGIERKTESLKSKKETGEKVQTLKVDIPSKVVWKAIPVLVTSLLLAALALY
                     FISPTSKKKQIEVVGNERLTAEQVENYSLISPDDYNVTVALHADAYAKNIKKNSSSVE
                     TATIKFQFPATFTIHIKEYAIIGYIQQQSQWYPVLSSGEIGGEPISQDSMPEGYTTIN
                     LSDKELIKELAIELGKIDAGIRSAIQTINLTPSKVTVDLLTFNMRDGNTVLVPLSEIS
                     QKVSYYTKIAAEVTVPTTIDMEVGIYRYA"
BASE COUNT         4054 a         2270 c         2739 g         3537 t
ORIGIN      
        1 ttttataaaa tattgctaac agttttccct tagacaaact catattacta gacaataaat
       61 ggaaactata gtaaaatacc aagtattgtt attaagttgt gccttaactt aattttataa
      121 tattttagta aaagagagga aacgcagatg gatgtaaaaa atcttcggaa attaaagcga
      181 attgagttat atgagattat gttggctcaa agtgaagaga ttgattgctt gagaaatcaa
      241 cttgagaaga ttaaaacaga gttggctgat agaagaatta tgattagtca atctggctcc
      301 atagcggaag cttcaatgaa actgactaat atttttgaag aagcgcaaaa agctgcggat
      361 ctatatctct ataatgtgaa aaataaaatt ggtgatgttc atgaatagtt caaacctcag
      421 gggaaagaaa cttccgaaaa cttccgattt acaagcagtt taccaacaag ctaaatataa
      481 aaagaccttt tgggaaacca tccgtagtac tgtatttatg cttgtagttg tagctgcttt
      541 tgctgtgctg attgctgtcc tttttcttcc cattctaagg atttacggga actcaatgaa
      601 agggaccctg aacagtgggg acatcgttgt gtccgtaaaa agtaatgatt ttgaaagcag
      661 tgatgttgtt gctttttatt acaataacaa tattctggtc aagcacgtca tcgctgaagc
      721 tggggattgg gtggatatgg ataaacaagg gaacgtttat gtcaataatc aaagattgga
      781 cgagccatat ttggcaaata gggattatag tcatacggat attgagtttc cttatcaggt
      841 accggaaaat cgaatctttg tcatgggaga taaccggaaa gagtctattg attcgcgaaa
      901 caacgctatt ggtacagtct ctaatgaaca gattgttgga aagttggtat ttaagatttg
      961 gccactgcct gaattgggat ggattgaata aatgaaagtg aaaaaataga aaggaggatc
     1021 ttgtgagaga ctggttaaat ttagaaaaaa tccaaggttg gcgacgaaag tacaagaagc
     1081 tcgtgaatat cattgctttc ctcgtagtat ttgcaacgac ctatgctttg attctacccg
     1141 ctatcacatt agaccaagaa aaggctgaac aaactgctgg gattgaagtg caagaaacac
     1201 cagtaacaac ggaaattagt aaattaacag aaccgatggt gacaactcag caaactccga
     1261 tagtgggtag ctctgctgat gaagttcaat ctcctgcgac agaagcggtt cctgaccctg
     1321 ctgagcaagt gtcaacagag tctgctagca aaccccagac cgaccaagaa cggattactg
     1381 agccaactac catagttcac aaaacgaatg attatgaggt gactgcaaca tttgatgcga
     1441 gtgctcaatt tcccaaagat gttgagttta atgttagaga actagatagc caatcagaag
     1501 aatatcggac acattatgaa aaaaccaagg agaagcttgg agtaaaaaac ttgtatatgc
     1561 ccgattcttt gatattcatt tttcatcaca gggccaagag gtcgagcctg ctgcccctat
     1621 aaagattacg attagaaaca aggaattagt tcaacggcca gatgatagta aactgaaaat
     1681 tgtccacttt gaagagaata gccaaataga agttttagag gccgaaacga aggaaaatga
     1741 acaacaaatc tccgagatca gttttgacgc tagccatttt tctgtttatg gagatatttt
     1801 agcaaactat tataccgtca aatttaccta cactgacaca gcgggtcaag aacaagtcat
     1861 tacgagtttg attgataaga ctgtggggac ttcacttggt agtttgcctg aagctccttt
     1921 taaaaacggt tatgtatttt ctcattggat taataaagat actggtgatc ggattgatga
     1981 aaatacggtg ataactggag acctgactgc tgtagcagta tttaataata tcaatatcta
     2041 caccattgat gtcacgtatt tctaccacaa tgtttctaaa aaccaagata ttacgattga
     2101 taaggaaacg gtgcaactgg aagcaagtga tgctccatat caactgacac cacctgcttc
     2161 tactgaggtg agcaacaaag aggatacaac tttaccagca gatgctattt actaccctga
     2221 aaaaccactt cttgagttta cagcagatca actagagcga ctggacgtag cagatggagt
     2281 ggatgataat catattagcg aaagagtaca atatgttcct tacaatgcag agtatgatgt
     2341 catttatagg ctaaagaatt tgacggggtc aggttattct gatatccaga ctgttcatgt
     2401 ccgtggggta ctgggttcaa ctgttacacc gcaaattcta acctacaact atgctgactt
     2461 tgaacgtgct gatacagcaa agattgagaa tactagcggc caaaaattgt atgtttatta
     2521 tacacggaaa acctttactc tttcttataa ttccaatggc ggtagtaata ttccccaaca
     2581 acagggtcta tacgatagcc agattaagat ttctacaact actccaacaa aagaggggta
     2641 tacatttgaa ggttggtatg ataatcctgg actgactgga aacaagatta caggctctct
     2701 caccttaaag aaagatacaa ctttgtatgc gaagtggaca ccgaacaggg ttcgctacat
     2761 cattgggtat tacaaggaag tctatgacaa caacactgga acaacctcct atgtatatga
     2821 tagttcggca gctggttggg gtctggttgg gactactgta aaaggaacag attcaaatat
     2881 tggacctcct ccgccaaccc aaacaaccat tggcagcatt ggctacgaga aggatgttac
     2941 taaaagtgct gcttcaagta ttgaaattgc agctgatgga acatctgttt tgaaagttta
     3001 ctataaactg aagcgctaca cttttgaatt tttcttaaat gataacaatg ccaatatcac
     3061 cataaatggt aagacctact ctgataggaa taggtacatg atacgtgatg ttgtgcttgg
     3121 tcaagatgtc tcaaagttat ggccagcttc tataacaaat cctaaagaag tatacgatga
     3181 taaagtctac tatgatagct atggtaggcc gataagatac ttttttgact actggatttt
     3241 tgggaatcta ctcttcaaaa cagatcggtt cgaagttgtt gagcctatgt tgccaacgag
     3301 tggaacaacc agtaagttca cagcatattg gaatacccaa ggtacacagg ctaacgttga
     3361 atattggtta caacaaccgg atggttctta taagaagtct gatatatata gtcaatcttt
     3421 cctaaagact gcaggactgc taccgaaaga aatttttgga tattcttatt atacagatgc
     3481 tagttttact gaagcaggca ttaggtatta cggaactcaa ggaaatacat atcgtttcta
     3541 ctataaacgc caagccttca aaattgacta ttattacaat agtactgggt tagattcaaa
     3601 gagtaacatt ctctttgaga gtgacatcaa tacagcaact tataattacc aaccaccacg
     3661 ttctactgga gttgatgctg attatacatg gggtggatgg tatacagatt cgttattgac
     3721 agagaagtat aactttaata agatgcctgc caataacttg gttctctatg ctaagtggat
     3781 tgctccaaaa tttaaggtta cttttgatat caacggtggt aatggaactg cccctgtcga
     3841 tcaaagtgtt gaaaaataca aatacgctaa ggcccctgct gatccaacga gggaacatta
     3901 caatttcctt ggttggtcta cagaagatgg gaaactctat agttggagcg accaagtaac
     3961 caaggatgtc aaattgactg cgaagtggga attgaaacca ttaacttata ctgttcgcta
     4021 tcttgaagca ggtacaaata cgcaattggc tgctgataaa gtgattacga gtccagcgct
     4081 tgtattgaat caagctatca cagaaaaggc attgggaatt acaggttatc gaccagataa
     4141 gagtagtcaa acaattaacc tgaattttag agggaacgaa ataactttct actatacacc
     4201 gaaggtaaaa gatgttgttt acaccatcaa gtatgtcttg aaggataacc cagctattga
     4261 ggtagcacct acagttactc gtaaggttga tggaagtatc atccgtgcga aagaagctgc
     4321 tgcagatgtg aataaacagc atttgcagag tcagtctggt gttactgctg aaatgctgga
     4381 aatggattac tatccaactg taaatactca aagtttgatt ctaacttcta atgatgcaaa
     4441 taatgtgatt gtttttgaat atgtcggatt cgatactcaa acctttacgg taaattattt
     4501 ggatatggat ggtaagaaaa tcgatggtca agaaccattg gtagtttacc gaaagaaacc
     4561 aggtagtttc gtggtagatc ataagactat aaatggttat acctatgatc attcgttaga
     4621 cagtgagaat gcagctgata agacagtata ccgtgtaagt aagggtggtc acataaccat
     4681 tgatctctat tataagaaaa acctaacact gaccgcatta gataagtcca aagtttatga
     4741 tggcactgcc ctatcaagtt cgggtctatc tgatctaaac cctgcttaca gtgtagacct
     4801 gaaagcagga gacaaattag ttgccatcgg ctatgatggt agccaaacca atgcgggtag
     4861 cagcgcaacg acacctaaac ttgctaacat cagcaatgct cttggtgctg accggacgaa
     4921 tttctatcac ataacctatc aagcgggtaa cttgagagtt gaccaacagc cagtcgtggt
     4981 tgtcatcaac ggtgaaaaga aatcgaaaat ctatgatggt aaagcggaag cggttggtta
     5041 taaggtgatg gagataagag atacaagtgg tctctataaa gaaacagata tccattttaa
     5101 tggttcaagt tcagagcagt cggtcaagaa aaaagatgca ggaatttatc cgctcctttt
     5161 aaataacaga ttcacaaata ccaatcagaa tttccaagta gatttcttga tttctgatgg
     5221 ggaattaaga atagatagac ggcaactgac cataacatct gattcagcaa gcaaggatta
     5281 tgatggtagt gagctccgtg ctgacaatat cactgtatca gtacctgctg gtgttgacta
     5341 cacaggtttt gtcgatggag aaggagttgt tccaaccttc ctaggcggac caaccgaacc
     5401 tggcttcatg cccaattact tcacctatca gcctaaagat ggaactaatc tggctaatta
     5461 tgagttggaa acacaatttg gtcagttgaa agtcaaagaa gttatcaaga ttcaaaagac
     5521 tgacttggct tggcaggctc tgtcaggtgg taagtttgaa cttaccaagt gggatggcct
     5581 caattgggct caggtagatg gtgcacaaga atttgcgatt accagcaaag atggcagcaa
     5641 tattcctgtg ggcttggaac ctggtcgtta ccgactccag gagttggcag caccggatgg
     5701 cttcattatc ctagatagct atatctactt caccatcaag gataacttca atgaagatca
     5761 gacaatgtcc ttctacacgg tcaccctatc tgatgaaatg ggcaatgatg ccactccaga
     5821 acgggcccag ttagaaagaa caacaggtga tgccagtcat cgtatcaagg ttgctaatga
     5881 gcaaggccga gctctgccaa gcactggcgg tagtggacag aaatggttta tcctttcagg
     5941 acttgttctc atggtcacct cctatctgat acatctgttt gttcagcgta atcggaaaat
     6001 gaggtcttag aactgagttt ccgcttgctt taatttcgtg tcgcaatgcg agttgcagga
     6061 tttcatgtct gcctctccat aataaaaaaa taaaaaagga aaaaatcatg aataaattta
     6121 aaagtctctt catgaccgtg ttggcagtgc tagctatggt cttgcctacc actgtcgcta
     6181 gtgcgcaaga ggtagataca aatgcaggtg gtacaggaac aattactgtg cgcaatgctt
     6241 cgcaaagcca aaaatatacc atctataaac tctttgatgc tactgtaaca gaagatggtt
     6301 caggtatttc ctacaaggtt ccgactggaa aaatacttcc agcagataat ccctactttg
     6361 ccgtggacag caaaggtaat attactgcaa aggaaggtgc tactgtctct tcagaagcat
     6421 ttgcggcttg ggcaaagaat ttcggtacag aagttacaag tgctacagcg aatgataact
     6481 ctcttgtgtt tacacatttg acatttggtt actattttgt aacatcatca cttggtgcag
     6541 tgcttacagt agatagtaca acaccaaatg caactgttat cgacaaaaat accactaatc
     6601 caaccattcc agattcaaat aatggtggcg gtaagaaaat tcttagcaat ggtgctacaa
     6661 ctagtgaaac aactgcaaaa attggcgata ccatcaactt ccaaatcaag tttaatgcta
     6721 ccaactacgt tactaaagat agacaaacca agcagattgt ttcttacgct atcgaagata
     6781 gcccaacagc cctcgcaatt gatcaaaatt ctgtcaacgt taaagtggat ggtgtggata
     6841 tcacagcaaa aatttcgaag acttttgatg caacaggcaa aatgaatctt gtccttacat
     6901 gggcggatgc ggcagctagt aataaaacca tctataactc tccagctgaa gttattatca
     6961 cctactcagc agtggtgact aaagatgcca aggagggaga agcaaccaac tcagcgacta
     7021 ttggttacaa cacaattgat aatccaacaa ctcctccaac accagttgat ccagacaagc
     7081 caactgaaac aacaaaagtg actactcacc gtttcacttt gaaaaaaaca aacaatgtag
     7141 gtgaaacttt gacaggtgca gagttcaaac tctatgatgc agccaacaat ggaactgaaa
     7201 tcaaggttgt caaggaatct gacggagtgt atcgtgttgc gcaggctgat gagcaaggtg
     7261 ttgtcatcga agctggcgaa gttgtgatta aaggtctgaa acatagcacg acctattatc
     7321 ttgaagaaat gaaagcaccg aatggttaca atatcttgac agaacgtcaa tccattgaag
     7381 tgaaagagaa taacacagca caagcaaata tcgtcaataa aaagggtggt gtcttgccaa
     7441 gcactggtgc gattggagca actttattct atctagttgg ttcaatccta cttttggtag
     7501 cattggttta tactatttca aaacgtcgta tgaacaatat ctaattgtcc atacaatacc
     7561 taatatttag aatctatctt cggaagtgca aaagcaagag tggaagtatt tccgaagata
     7621 tttctttggt ctcaacacaa ggaaaggagg ggatttcatg atcaaatggc tcaaaaagaa
     7681 aggtccgtct tttatcatgc cattaatttt tatattgggg attggactat tggcttatcc
     7741 atcggtcagt gattactgga attccttcca ccaatcagag gccatcatgt cctattctga
     7801 cacagtatca gatatgacag ctgaccaata caaagaaatc attgatagtg cgcgccagta
     7861 taatcaagaa aaacccttaa actggaatgt gactgcatcc gacattgaag cctataatca
     7921 ggaattgaat ttcaataaag atggcatcat gggctatatt gagattccta aaattgatgt
     7981 caagttatct atatttcatg ggacagacga aacggtttta gagacttcta ttggacatct
     8041 agaaggaaca tcgttgcctg taggaggttt gggaactcat agtgtacttt cagggcatcg
     8101 tgggctccca tctgcccgtc tgttttctga tttagataag ttgagagaag gcgatatatt
     8161 taccattcat gttctcaatg aaacactaac ttacaaagtg gaccagattc gagtggttga
     8221 gccatcagat ttatcggctc ttaccataga acctcagaaa gacttattga ccttggtaac
     8281 ctgtacaccc tacggtatta atacacatag gctattagtt cgtggctatc gtattgaaaa
     8341 tgttaatggt agtgctttgg ttacctctga tgctatccaa attaaagcaa tttttattgc
     8401 tccatttatc gcaactccaa ttctatttgt aatcttaatt tatattttta taacaactag
     8461 taaaacattt cgttcttgga ataggaatca agtgttggat gattttttag ttcccaagaa
     8521 attttgaact attgaaaatg tttcaaagtc gatttgactc tggaacattt ttctattata
     8581 gacattagac aatatggctg aatgaggtaa ttgttcaact tctaacctcc tgtcaactca
     8641 aatcaattgc ctagaccgac gattgaaacg acttctttct agtaacggac cacgaggaaa
     8701 gaggtggcag atattgtaga aatgtcgttg agatgtcata cctagttcag cttgtaacaa
     8761 aagcaagaca aggagtaatc tatctgaaag cctacattac gccgaagtta aaaggaatct
     8821 gggcaatagt tttggtagaa atgatgagaa atttgtgata attggcgtac attccattgc
     8881 aaatgatgag atttagcagt atactgtaag tggctcattt ggactacccc tctgtatgtt
     8941 tcgtcgctta cagtatagtc catttgggct ttttattgta ttttgaaatg aactagcgct
     9001 acgggtagag tttacttttc tgtcctctgg cttgaaaaag gtcttcgtaa agggtaaaat
     9061 aaggtgttag aaagtaaagg taattgagat gaagatgatt gatattgtaa aaaataagaa
     9121 agtgctagtg ctaggtttag ctaaatcagg ggaatctgca gcccgtctcc tagataaatt
     9181 gagtgctatc gtcactgtta acgatggaaa gccttttgaa gaaaatccga ctgctcaatc
     9241 tttgttggaa gatggtatcc gtgttatttg cgggagccat cccttggaat tattggatga
     9301 agatttcgct ttgatggtaa aaaatccagg aattcgctat gataatccta tggttgaaaa
     9361 agcaattggc aaaggaattc cagtatggac agaggttgaa ttggcctact tggtatcaga
     9421 tgctccaatc gtggggataa caggttcgaa cggcaagaca acgacaacga ccatgattgc
     9481 agaagtcttg aatgcaggaa ataaacctgc aaaattatgt ggaaatattg gctatccagc
     9541 atcttcggtc gctcaaactg cgactgcaga ggataccttg gtgatggaat tatcatcttt
     9601 ccagttaatg gggacagaat ctttccgccc gcatatcgcc gttgtgacca acctcattgc
     9661 cagtcatatt gattatcatg ggacttttga agactatgta gcagctaaat ggatgattca
     9721 gggtcagatg gcggctgagg attttgtggt gctcaacttc aaccagaaag aagctaagga
     9781 attggctgaa cagaccaaag caactgtagt tcctctttct acgctggaag tagttgatgg
     9841 agcttacttg gcagatggaa aactctattt caagggtgaa tacatcatgg atgctgacca
     9901 aattggtgtt ccaggttcac acaatgttga aaatgccttg gcaacaattg tggttgcaaa
     9961 attgttggga gtagatcgcc aagcgattca ggaaagtttg tctgcctttg gaggtgtcaa
    10021 acaccgcttg caatttgtag gtgaaatcaa cggggtttcc ttctacaatg atagtaagtc
    10081 taccaatatc ttggcgactc aaaaagctct ttcagggttt gacaatagca aggtcatttt
    10141 gattgcaggt ggcttggacc gtgggaatga atttgatgaa ttagtccctg atttggtcgg
    10201 actaaagaaa atggtgatcc ttggtcaatc agctccccgt gttcaacgtg ctgcagataa
    10261 ggcagaagtg gaaacgatag aagctctcga tattgcggat gcgactagaa aagcctttgc
    10321 cattgcagaa aaaggagata ttgtcctttt aagtccagcc aatgcaagct gggatatgta
    10381 tgccaatttt gaagtgcgtg gcgacgtctt cttacagacc tttgaggagt tgaaataatc
    10441 atgaaaaaaa ttgtttttac aggcggaggg actgttgggc acgtgacgct caatctcctt
    10501 ttgattccgc gatttttgga agaaggctgg gaagtccact atattggtga tgggaacggt
    10561 attgagcatg agcaggttgt gaaatcaggt ttagatgtcc attttcattc tatttcgact
    10621 ggaaaattgc gccgttattt ctctttccaa aatatgttgg acgtgtttaa agttggtttt
    10681 ggtgttcttc aatccgtggc cattattgct aaaattcgtc cgcaagctct tttttcaaaa
    10741 ggtgggtttg tatcggttcc gccagttatt gctgctaatt tgctgcgggt tccggtcttt
    10801 atccatgaat cagacttaac aatgggactg gccaataaaa tagcctataa atttgcgaca
    10861 accatgtata gtacttttga gcaacctgct agcttgacta aagtaaaaca tgttggtgcg
    10921 gttacaaaag tggggaaaac gaatgacaaa gttacaccaa tccagttgcc agaaattctc
    10981 agtcacttcg ataaaagtct gccgacccta ctgtttgtag gtggttcggg cggtgctaag
    11041 gtctttaatg atttaatcag tcagaatagt gctgctctga ccgaacgatt caatattatc
    11101 aatttgacag gggatagtag tctcaaccaa ttagataaaa acctatatag agttgactat
    11161 gtgacagaac tttatcaacc gttgatggat ttagcggatg tcgttattac tcgtggtggt
    11221 tccaatactc tgtttgagtt gattgccatg caacaactcc atttgattgt gcccctagga
    11281 cgacaggcca gtcgagggga tcagattgaa aatgctctct atgcagagaa aaagggatat
    11341 tctaagcaga ttgatgaaag tcaattgaca tttgccagcc tgttagtaga agtagatgag
    11401 ctattgaaaa ataaggaatt ttatatccaa aacatgacaa attctaatga aattcagtca
    11461 gtagatagtt tttacaattt gcttagagaa gatatgggaa ggtaggtcct atgacggaaa
    11521 aagattcaaa tgtagaagaa tcagtgctag aagtggaaca ggcatctcaa gtagaattgg
    11581 attcagaaca aatctcacca gccgagaaag aatctgtatt agccgaagaa aaaggacttt
    11641 ctacggatgt ttctacggat gtagacatac ctgagatgac tgctccggat gatgaaaaaa
    11701 gtgccttttt cgaacaatgg aaagcacgcc atcaagccta ccttgctcat aaagatgctg
    11761 cggaaattca ggcagttgac gagggacaga cagaacagga gaacccagaa gctaaaaaga
    11821 gtaaaagggt cctttttcag ggaatagaaa gaaaaacaga gagtctgaaa tctaaaaagg
    11881 aaacagggga aaaggttcaa actttaaaag ttgatattcc ttccaaagta gtctggaaag
    11941 ctattccggt tcttgtgacg agtttgttac tggcagctct agccttgtat tttatttctc
    12001 caaccagcaa gaaaaaacaa atagaggttg tcggaaatga gcgactaact gctgagcaag
    12061 tagaaaatta tagccttatc tctccggatg attacaatgt gactgtagct ttgcatgcag
    12121 atgcttacgc aaagaatatt aagaagaata gttcgtccgt tgagacagcg acaatcaagt
    12181 tccagtttcc agctaccttt accattcaca tcaaagaata cgcaataata ggctacattc
    12241 agcaacaaag tcagtggtat ccagtattgt ctagtggaga gattggcggt gaaccgattt
    12301 cacaggattc catgccagaa ggctacacaa cgataaattt atccgacaag gaattaatca
    12361 aggaactggc cattgaattg ggtaaaatag atgcaggaat tcgttctgct attcagacaa
    12421 taaatttaac gccaagtaaa gtgaccgtgg atttgctgac attcaatatg agagatggaa
    12481 atactgtatt agtcccgttg agtgagatta gccagaaggt gtcctactat acaaaaatag
    12541 cagcagaagt gactgttcca acaacaattg atatggaagt tggtatttat agatatgcga
//
LOCUS       DBNFEG010000046        12457 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.046, whole genome shotgun sequence.
ACCESSION   DBNFEG010000046 DBNFEG010000000
VERSION     DBNFEG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12457
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.046"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(310..1404)
                     /locus_tag="AC1PNG_000891"
     CDS             complement(310..1404)
                     /locus_tag="AC1PNG_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIP6701402.1"
                     /translation="MFHFTTLFIGMDVHKESFLLCYYDMMANQFKHSTKVGPNVSYIV
                     NYVNELRRLYGQDAEVLCGYEAGCLGFTLYHQLQAHGIPCIVMAPTTVMKEGSKRVKT
                     DKKDAAQLAKALAFRSYRPVHIPTAEDEQVKEYIRMRTDHKVALKKIKQQLLAFCLRH
                     DFRYTEGRNNWTQKHVRWLHSLNPKGLYAEILTEYLLTYEKLVDQIARYDARIEQLGQ
                     SDSYQEEVSQLSCFIGVKTLTALSIVTEIGDFNRFATAQHSASYLGLTPSENSSGDKE
                     RRGAITKAGNSHVRRLLIEAAQSLAKGTIGYKSKELKRRQSGNRVEVIAYADKANERL
                     RRRYRTLVLGKNKKQNVAKVAIARELSGFI"
     gene            complement(1512..2186)
                     /locus_tag="AC1PNG_000892"
     CDS             complement(1512..2186)
                     /locus_tag="AC1PNG_000892"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIP6701403.1"
                     /translation="MLKFIKHIASLFLFFVAYQIISGFLMVGPSLQAIPEFPAQLIEN
                     MIWICAIIGIVLGIAFTIVLWKFVYSRYTIDYSVSSSWFHKIQWPILLYIAFFIFQLL
                     VPISESQNQTLVIQFVSAYPLVSFLSVVIFAPILEELIFRGLLATYFFPKMVDMKAVG
                     IYLAVTGSLFSLVHMPTTILQFLIYFTMGLNLGWLYLIRRDIRYPIALHMLNNGISYL
                     MILFLV"
     gene            complement(2196..2513)
                     /locus_tag="AC1PNG_000893"
     CDS             complement(2196..2513)
                     /locus_tag="AC1PNG_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701404.1"
                     /translation="MKLFLWILVGFLFFNSLSDRKKVKQLQARVKKLQKTTKGENQMS
                     VLLKELVGSKAKIRFDEEISIACEYTILAVDEEWVKISRELAEGEIETKLVRVDNIVD
                     LSF"
     gene            complement(2523..3212)
                     /locus_tag="AC1PNG_000894"
     CDS             complement(2523..3212)
                     /locus_tag="AC1PNG_000894"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIP6701405.1"
                     /translation="MNRLGNVKDWNVVKNWKFLLVGLAVVALNVLTQLAMFALPNFDG
                     NNLLIAAVLLLLAVVLGLILTGKLGLWKGEQKWGMVANVGFVVLAFIVMFGLKIIGGQ
                     LIMLEEGYGQTTANQEIINNSGLPTLVLFLFAVFFAPVLEELLFRGILMGRVFGKDSI
                     VGLLLSSFLFGLIHNPTNIGSWVVYGGMGLVLGLAYRISGKYTNALILHSLNNLLGFL
                     LMLLMQSLGLI"
     gene            complement(3238..3444)
                     /locus_tag="AC1PNG_000895"
     CDS             complement(3238..3444)
                     /locus_tag="AC1PNG_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701406.1"
                     /translation="MKENKKFISVLKIVYFVGLIGLGLYAVGYIFGEWLATGQIDLSN
                     LNILLPMVLGLPALLLFKKESDKN"
     gene            complement(3466..4224)
                     /locus_tag="AC1PNG_000896"
     CDS             complement(3466..4224)
                     /locus_tag="AC1PNG_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HIP6701407.1"
                     /translation="MKQGKQISTKQRWMRYSAYMLISAILGGFIGFFGVLIFKFSLPS
                     YLNLDNFLIFLRIITFLIFAGTVYFGVKANQNYKLYHSISDEDEEHVDELYKKMYRNL
                     EYATISFNVAVSLTLLNLVLGFGVTFFEDGAIMYGSIFDVVFYVILLISQIFIMKLTQ
                     KIRDYKLSAFATVKEMKDFAEAMDEGEKQANYEMSFQIVFTLNQIVLPGLYLFLFVLS
                     MLLQERQITAFLVVAFLHIYINVMQVRMIRRYFK"
     gene            complement(4221..4436)
                     /locus_tag="AC1PNG_000897"
     CDS             complement(4221..4436)
                     /locus_tag="AC1PNG_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIP6701408.1"
                     /translation="MEYVLKNRLKELRARDSLNQTELAKLAEVSRQTISLIERGEYTP
                     SVVIAMRIAQIFNENVENVFQLVEVGE"
     gene            complement(4449..5015)
                     /locus_tag="AC1PNG_000898"
     CDS             complement(4449..5015)
                     /locus_tag="AC1PNG_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HIP6701409.1"
                     /translation="MEAKKKLFEVNIMKNKKFVWGLNILAVALILPFAFVFGMIGSWL
                     IANGSQYWELSVSEVWLLFPLYILLIVVHEAIHGIFFKVFCPENPVKYGFKWKSGMAY
                     ATSPGSLYNRMQMLVISLAPFVVISLGLTMLAGFGMMNTTLYFMLATMHAASCAGDFY
                     YVYLLLVKFAKENIAVEDTETGLIIYQA"
     gene            complement(5166..5360)
                     /locus_tag="AC1PNG_000899"
     CDS             complement(5166..5360)
                     /locus_tag="AC1PNG_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701410.1"
                     /translation="MKQPFIITGTTIARAVEIGSILLLAYILLFDLLGWSDMDTALMT
                     TLIASITCIEASLQMEKKRR"
     gene            complement(5564..6016)
                     /locus_tag="AC1PNG_000900"
     CDS             complement(5564..6016)
                     /locus_tag="AC1PNG_000900"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase family protein"
                     /protein_id="HIP6701411.1"
                     /translation="MEHIKDIWQEMYEKAKGHYAPHYVSPFIYSNHVVAAIEAEDGQI
                     YTGYCFEATAGVFHLCAERAAAFNMFQDSGQTTIKRIIAFRNQAPSGEGSGMPCGACR
                     EFLMQLSAENAQTEIMVDFDKRETITLGELMPYWWGEERMQAGTDHLE"
     gene            complement(6091..8709)
                     /gene="alaS"
                     /locus_tag="AC1PNG_000901"
     CDS             complement(6091..8709)
                     /gene="alaS"
                     /locus_tag="AC1PNG_000901"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIP6701412.1"
                     /translation="MKNLSSAQIRQMWLDFWKSKGHSVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPDNPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPEWFAFPKDKLYMTYYPDDMDSYNRWIALGVEPSHLIPLEDNFWEI
                     GAGPSGPDTEIFFDRGTDFDPENIGIRLLEEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAIFQGAKTNFETDLFLPIIREVEKISGKTYDQDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRASMHGQRLGITEPFLYKLVETV
                     GNIMESYYPEVLEKRAFIEKIVKSEEESFARTIHTGSQFAEQLMDKLAAEGKSEIDGR
                     DIFKLYDTYGFQVELTEELAEHRGMTLDIAGFEAAMKEQQDRARASVVKGGSMGMQNE
                     TLAAITEESTFVYGQTALEASLSVIVADNARTEAVSEGQALLVFDQTPFYAEMGGQVA
                     DHGFVKNATGDIVATVIDVQKAPNGQPLHTVELSASISVGQTYTLEIETKRRKGVEKN
                     HTATHLLHAALHNIIGEHATQAGSLNEQDFLRFDFTHFEAVTAEELRRIEEEVNEQIW
                     NAIPVVTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSVELCGGTHVGNTAEIGI
                     FKILKEEGIGSGTRRIIAVTGREAFLAYRDQEDALKEVAATIKSPQIKEVPNKVESLA
                     QQVRDLQKENAALKEKAAAAAAGDVFKDVKDANGIRYIASQVQVSDAGALRTFADNWK
                     QKDYSDVLVLVAAIGDKVNVLVASKSSDVHAGNLIKVLAPIVAGRGGGKPDMAMAGGS
                     DSSAIQTLLNSVAENL"
     gene            complement(8989..9474)
                     /locus_tag="AC1PNG_000902"
     CDS             complement(8989..9474)
                     /locus_tag="AC1PNG_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HIP6701413.1"
                     /translation="MKQFQVKQRFTFGGEKFDIRDSFGNLAYQVEGSFLEIPKRFIVT
                     NSRGGEVCQITKKFLTFLPQFTIDMVEGHSFYLQKEFTFFKDRYTVENLGLILDGNIW
                     DLDFRLKRPDGMLVAEISKELFHLTSHYSVTVLEDSYADLVISLVVAIDYIEMMEDAS
                     N"
     gene            complement(9739..10740)
                     /gene="prsA"
                     /locus_tag="AC1PNG_000903"
     CDS             complement(9739..10740)
                     /gene="prsA"
                     /locus_tag="AC1PNG_000903"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HIP6701414.1"
                     /translation="MKQTKKILAGAVTLFAAVTLAACSNAADKDIITMKGNTITVSEF
                     YEKVKTNSQAQQVLLSMVISNVFENQYGDKVSAEEVNKEYDKKAEQLGASFNAALSSA
                     GLTEESYKEQIRTNKLVEYAVKQAAEKELTDENYKAAYDAYTPEVTARVIKLADEAKA
                     KEVLAAAQAEGADFAQLAKDNSTDTTTKDNGGEVKFDSTSTTVPAEVQKAVFALDAGQ
                     VGASVISSVDMKTYTTSYYVVKLDAKSEKSAKWEDYKDKLKEIILAQKQRDSSFVATV
                     LKEALQKANVKVKDSAFQNLLSQYVTTEESSSSSTKSSSSSTESSSSTTESSSSSGQ"
     gene            complement(10801..11520)
                     /locus_tag="AC1PNG_000904"
     CDS             complement(10801..11520)
                     /locus_tag="AC1PNG_000904"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIP6701415.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDFMRTRQAQNTGFLKELEEFA
                     HQENIPVIPHETVAYFRLLMQTLQPKRILEVGTAIGFSALLMADNSPHSKITTIDRNE
                     EMIGFAKENFAKYDYRQQIELLEGEAMDILPTLPDNTYDFIFMDSAKSKYIVFLPEVL
                     KKVKVGGLIILDDIFQGGDVAKDIKDIRRGQRTIYKGLQRLFDATLDNPDVTASLVSM
                     SDGLLMLRKNVENVDLKHIEI"
     gene            complement(11595..12143)
                     /locus_tag="AC1PNG_000905"
     CDS             complement(11595..12143)
                     /locus_tag="AC1PNG_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6630 family protein"
                     /protein_id="HIP6701416.1"
                     /translation="MLTEEQLQDIFELADLMSNGDRELFLQLREVVFASDPHEILNFM
                     ERILDSESFDDFLDRVGESEKENLWLILIKLLEHLNYICVRDYKDNLEDFIYFFDCLQ
                     QVRNAGISLKLDSGGLSPIASISEWARVIDNKYLDENFCLGAVDMDTDSYYLFFSKQA
                     TFIRLQELAGNLGYRIDYAKNM"
     gene            complement(12159..>12457)
                     /locus_tag="AC1PNG_000906"
     CDS             complement(12159..>12457)
                     /locus_tag="AC1PNG_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701417.1"
                     /translation="LYILRKQKAVFILTKQYLEFQEHFWSERQRHSLEEIAEIRYIVS
                     PVYRGYQSKRLRIVGNKGGLLAVVSLNRLDGADFNDIYHFVEQVAPHIRWDFSN"
BASE COUNT         3809 a         2789 c         2169 g         3690 t
ORIGIN      
        1 tagcaacctg tagctttggt tacctacagg tcaatggttc tgccgcgacg cgagtccgac
       61 tctctataag cgtggattac aattgtgcca cttagtcgtt tcgtagatga ctcaaacctt
      121 gaagtcctaa actccttcaa gatactttcc attcaaacat tatttcaatc cttatttctg
      181 tccaaattca cccagtgatt tgggatagaa atagggattc ctcatcgctc tgcgatgata
      241 aaacttaatt gtcaaaagtg tacatgaaaa taagcgctaa cttcaagcta ttcttcctgt
      301 catcatcccc taaataaaac cagataattc ccgtgcaata gctactttag caacattttg
      361 tttcttattt tttcctagaa caagtgtgcg ataacgtctt cttaagcgtt cattagcctt
      421 atccgcataa gcaatcacct ccactcggtt tccactttgt ctccttttca attctttgga
      481 tttataccca atcgttccct tggccaatga ttgtgcagct tctatcagaa gtcgtctcac
      541 atggctattc ccagctttgg tgatagcacc tcttctctcc ttgtcgccac tagagttttc
      601 gctaggagtc agcccaagat aagaagcaga atgttgagct gtcgcaaagc ggttaaaatc
      661 accgatttct gtcacaatgg aaagagcggt tagtgttttg acgccaataa agcaagaaag
      721 ctgagagacc tcctcttgat aactgtcgct ttgacccagt tgctcaattc gcgcatcata
      781 ccgtgctatt tgatctacta atttctcata ggtcaatagg tattctgtca aaatctctgc
      841 gtaaagtccc ttaggattta gggaatggag ccagcggaca tgtttctgtg tccaattatt
      901 tcttccctcg gtatagcgaa aatcatgtcg gagacagaag gcaagaagtt gttgtttgat
      961 tttcttcaga gccactttgt ggtctgttct catgcggata tattctttga cttgttcatc
     1021 ctcagcagta ggaatatgaa caggccgata gctacgaaag gccagagctt ttgcgagctg
     1081 agctgcatct ttcttatcag tcttgacacg cttagatcct tccttcatca ccgttgtagg
     1141 cgccatcacg atacagggaa tcccgtgagc ttgtagctgg tgatataggg taaatccaag
     1201 acatccggct tcgtagccac ataacacttc tgcatcttga ccatataaac gacgaagctc
     1261 attcacatag ttcacaatat agctaacatt tggaccaact ttagtgctat gtttgaattg
     1321 attcgccatc atatcataat agcagagtaa aaaactttct ttgtgaacat ccattccgat
     1381 gaaaagtgtg gtaaaatgaa acatataaga cctccaattg agtgtggtaa ttcctgttag
     1441 aagttgattg ttttttattc tagtgtacag gtaaatccac gaattctcaa ctgggggtct
     1501 ttacatattg tctataccaa aaatagaatc atcaaatagg aaattccatt atttaacata
     1561 tgtagcgcaa tcggatagcg aatatctcgt ctaattaaat agagccaacc aagattgaga
     1621 cccatagtga aataaattaa aaactgcaga atggttgttg gcatatggac caaactaaac
     1681 aaagaacccg ttacagcaag atagattcca acagccttca tatctaccat tttagggaag
     1741 aaataggtcg ccaacaaacc tctaaaaatc aattcctcta aaatcggagc aaagattacg
     1801 actgataaaa atgaaaccaa ggggtaggca gacacaaatt ggatgaccaa ggtttgattt
     1861 tgactttcag atataggtac caaaagttgg aagatgaaaa atgcaatata gagcaaaata
     1921 ggccattgaa tcttatggaa ccatgatgaa gatacactat agtcaatggt ataccgagaa
     1981 tacacaaatt tccataatac aattgtaaat gcaattccga gaacaatgcc aataatcgca
     2041 caaatccaaa tcatattctc aatcaattga gctggaaatt ctggaatagc ttgtaatgac
     2101 ggccctacca tcaaaaaccc agaaataatt tgataagcaa caaaaaataa aaacaatgat
     2161 gcaatgtgtt ttataaattt taacatactc cctccctaaa aactcaaatc aacaatatta
     2221 tcaactcgta ccaacttggt ttcaatctct ccttccgcta attcacgaga aattttaacc
     2281 cattcctcat caactgccaa aatggtgtat tcacaagcaa tagaaatttc ctcatcaaag
     2341 cggattttgg ccttacttcc gaccaattct ttcaataaaa ctgacatctg attttctcct
     2401 tttgtagttt tctgcaactt ttttacccta gcttgcaact gcttcacttt tttcctatct
     2461 gatagagaat tgaaaaatag aaatcctact agaatccata aaaatagttt catcgtatcc
     2521 tcctatatca agcccagact ctgcatcagt agcatcaaca aaaatccaag aagattattc
     2581 agactgtgta gaataagtgc attggtatat ttgccagaaa tacgataggc gagtcccagg
     2641 accaggccca tacctccgta gacaacccaa gatccgatat tggttggatt gtgaataagt
     2701 ccaaacaaaa aacttgatag caataatccc acaatcgaat ccttaccaaa tactctcccc
     2761 ataagaatcc ctctgaaaag cagttcctcc aaaactggag caaagaaaac tgcaaagaga
     2821 aacaaaacta aggttggtaa gcctgaattg ttgatgattt cctgattggc agtcgtttga
     2881 ccatatccct cttccaacat aatcaattgc ccaccaatta ttttcagacc aaacataacg
     2941 ataaaagcga ggactacaaa cccaacattc gcaaccatcc cccatttctg ctcacctttc
     3001 catagaccta atttaccagt caagataaga ccaagcacca cggctaacaa taaaagaact
     3061 gcagcaatca gtagattatt accgtcaaaa tttggtaggg caaacatggc caactgggtc
     3121 aatacattca gtgcaacgac tgccagtccc actaagagaa acttccaatt tttaaccaca
     3181 ttccaatctt tgacatttcc aagtctattc atagcaatct ccttcacata cactaaatca
     3241 attcttatca ctttcttttt tgaacaatag cagagctggc aagcccaaaa ccatcggcaa
     3301 gaggatattc agattagaca aatcaatctg cccagtagcc agccattctc cgaaaatata
     3361 gccaactgcg tacagaccga gaccgattaa ccccacgaag taaacaattt ttagaaccga
     3421 tataaacttt ttattctctt tcataatcac taccaaaacc ttcccttatt taaaataccg
     3481 acggatcatg cgaacctgca tcacattgat ataaatgtgc aaaaacgcca caactagaaa
     3541 tgccgtaatt tgtcgttcct gcaatagcat gctaagtaca aataggaata gatacaaacc
     3601 tggtaacacg atttgattca aagtgaagac gatttggaaa ctcatttcgt agtttgcttg
     3661 tttctcacct tcgtccatcg cctcagcaaa gtccttcatt tcttttaccg tagcaaaagc
     3721 ggataactta tagtcacgaa ttttctgagt taatttcatg ataaaaatct gagaaatgag
     3781 aagaatgaca tagaaaacta catcaaaaat tgacccgtac atgatcgccc cgtcttcgaa
     3841 gaaggtaaca ccaaatccta gaaccaaatt taacaatgtc aatgatacag caacattgaa
     3901 tgaaatggtc gcgtactcta ggttacgata cattttctta tatagttcat caacatgttc
     3961 ctcatcctca tctgaaatgg aatgataaag tttataattt tgattagctt taacaccaaa
     4021 atatacggtt ccagcgaaaa ttaaaaatgt aataatcctc aaaaaaatta gaaaattatc
     4081 taaattgaga taagaaggta ggctgaattt aaagattaat actccaaaga aaccgataaa
     4141 gccacccaaa attgctgaaa tcagcatata cgctgaatat ctcatccatc tttgtttcgt
     4201 tgaaatttgt ttcccctgtt tcattctcct acctccacta attgaaaaac attctccaca
     4261 ttttcgttaa aaatttgagc aattcgcatg gcaatgacca ccgagggcgt gtactccccc
     4321 cgctctatca gactaatggt ctgccgtgat acttctgcta actttgccaa ctcagtctgg
     4381 ttgaggctat ctcgtgcccg caactccttg agccgatttt ttaagacata ttccatgatg
     4441 tttcctttct aggcttggta gataatcaag cctgtttctg tgtcttcgac agctatattt
     4501 tccttggcaa acttgaccaa taagagatag acataataga aatcgcctgc acaagaggcc
     4561 gcatgcatgg ttgccagcat gaagtaaaga gtggtattca tcatcccaaa tcctgctaac
     4621 atggtcaagc ccaaactaat cacaacaaag ggagccaagc tgattactag catttgcatc
     4681 cgattgtaga gcgaacctgg actggtcgca taggccatac ccgatttcca tttaaacccg
     4741 tatttgacag gattttcagg acaaaaaact ttgaagaaga tgccgtggat agcctcatgc
     4801 acaactatta aaagaatata gagtgggaaa agcaaccata cctccgacac gctcagttcc
     4861 cagtattgcg aaccgttagc tatcaaccaa ctgcctatca ttccaaatac gaaggcaaat
     4921 ggaagaatga gtgccacagc aagaatattc aatccccaca caaacttctt atttttcatg
     4981 atattgactt caaataattt ctttttcgct tccatgtttc ttctccttca tttgcctaca
     5041 agctagacta aactatatag tcctagagca actccaatca gtaagccgat aaataattgc
     5101 ccctgcaatc ataatcgcta ccatatctgc tgaaatatta cgtttcatgg tctatctcct
     5161 attacttatc tccttttttt ctccatttgt aaacttgctt caatacaagt aattgaagca
     5221 attagagtag tcattagagc tgtatccata tcgctccatc ccagcaaatc aaaaaggaga
     5281 atgtaagcca ataatagaat ggaccctatt tcgactgcac gcgcaatggt tgttcctgta
     5341 attataaaag gttgtttcat ttggtgactc cttatctgtt catcgtatca gatgaacctt
     5401 ttcgattttc atctcaatat cattgtaaca cttctttttc tttttgacaa ctatctttgt
     5461 caaaaaaata ataatttttg tcaaaaagtt tatgaatgtt gtcaaattga ctagaatgcc
     5521 atacaaaaaa ctcctttatc cagaggataa aagagttcat ttattattcc aagtgatctg
     5581 taccagcttg cattctttct tcaccccacc agtaaggcat gagttcgcct aatgtgatgg
     5641 tttctcgttt gtcaaaatca accatgattt ctgtctgtgc attctcagcc gacagttgca
     5701 tcaaaaattc acggcaagct ccacaaggca ttcctgaacc ctctccagag ggagcttgat
     5761 ttcgaaaggc aataatgcgt tttatcgtcg tctgcccact atcctgaaac atattaaagg
     5821 cagcggctct ctctgcacaa agatgaaata cacctgctgt tgcctcaaag caataaccgg
     5881 tgtaaatttg tccatcttct gcctcaattg cagcaacaac atggtttgaa taaatgaaag
     5941 gcgaaacata atgtggcgca taatgacctt ttgctttctc atacatctct tgccaaatat
     6001 ctttgatgtg ttccatacgt actcctcatt ttagaataga aagatatccc cttgctagtt
     6061 gctttaacaa agggatatac actgactata ttacaagttt tcagctacac tattcaagag
     6121 ggtttggata gcagaggaat cgctaccacc ggccatggcc atgtccggct taccaccacc
     6181 acgacctgct acgattggcg caaggacttt gatgaggtta cctgcatgaa catcgcttga
     6241 cttgcttgct acaaggacat tgaccttgtc gccgatggcc gcaaccaaca ccaagacatc
     6301 agagtagtct ttttgcttcc agttgtcagc gaaggtacgg agggcacctg cgtctgaaac
     6361 ttggacttgg ctagcaatgt agcgaatgcc gttagcatct ttgacatctt tgaagacatc
     6421 accagcagcg gctgccgctg ctttttcttt cagagctgca ttttcttttt gcaggtcacg
     6481 aacttgctga gcaagacttt caaccttgtt tggcacttcc ttgatctgcg gcgacttgat
     6541 ggttgccgct acttctttga gggcatcttc ttggtcacga taggcaagga aggcttcacg
     6601 gcctgttacc gcaatgatac ggcgagtacc tgaaccgata ccttcttctt tcaaaatctt
     6661 gaagataccg atttcagcgg tgttgccaac gtgggtacca ccacagagtt ccacagagta
     6721 gtcaccgata gtaacaacgc ggacttcttt accgtatttt tcaccgaaga gggccatggc
     6781 acccatttcc ttggctgtgt caatatctgt ctcaactgtc acaactggga tagcattcca
     6841 aatttgctca ttgacttctt cttcaatacg acgaagttct tccgcagtaa ctgcctcaaa
     6901 gtgcgtaaag tcaaagcgga ggaaatcttg ctcgttcaag gaaccagcct gtgttgcatg
     6961 ctcaccgata atattgtgaa gagctgcatg gagcaagtga gttgctgtgt ggttcttctc
     7021 aacaccctta cggcgttttg tttcgatttc aagtgtgtag gtttgaccaa ctgaaatact
     7081 tgcggacagt tcaactgtgt gcaatggctg accattcggt gctttctgca catcgatgac
     7141 agtcgcaaca atgtctccag tagcattttt cacaaaacca tggtctgcaa cctgaccacc
     7201 catttctgca tagaatggtg tttggtcaaa gacaaggagg gcttgacctt ctgagactgc
     7261 ttctgtacgc gcattatcag ccacaatgac agataagctt gcttcaagag ctgtttgccc
     7321 ataaacaaag gtcgactctt ctgtgatagc agccagggtt tcattttgca tccccattga
     7381 accacctttg acaactgaag cacgggcacg gtcttgttgc tctttcatag ccgcttcaaa
     7441 accagcaatg tcaagggtca taccacggtg ctcagccaat tcttctgtca attctacttg
     7501 gaaaccatag gtatcataga gtttgaaaat gtcacgacca tcaatctcag acttaccttc
     7561 tgctgccaat ttatccatca attgctcagc aaattgtgaa cctgtatgga tagtacgagc
     7621 aaatgactct tcttcactct taacaatctt ctcaatgaag gcacgttttt caagtacttc
     7681 tggatagtaa ctttccatga tattaccaac tgtctccacc aatttgtaca agaatggctc
     7741 tgtaatgccc aaacgttgac catgcataga agcacgacga aggagacgac gaaggacata
     7801 accacgacct tcatttccag gaagggcacc gtcaccaata gcaaaagaaa gggcacggat
     7861 gtggtctgca ataaccttga agctcatgtt gtcgccatct tggtcatagg tcttaccaga
     7921 aatcttttct acttcacgaa tgattggcaa gaagagatcc gtttcaaagt tggtcttagc
     7981 cccttggaaa atagctgcta aacgctccaa acctgcgccc gtatcgatgt ttttgtttgg
     8041 caattccttg tactcagagc gaggaacagc tgggtctgcg ttgaactggg acaatacgat
     8101 attccaaatc tcaatgtaac ggtcattttc aatatcttct tccaagagac ggataccaat
     8161 gttttcaggg tcaaagtcag taccacggtc aaagaaaatc tccgtatctg gaccagaagg
     8221 acctgcaccg atttcccaga agttatcttc aagcggaatc aagtggcttg gctcaacacc
     8281 aagggcaatc caacgattgt atgaatccat atcatcagga tagtaagtca tgtagagctt
     8341 gtcttttgga aaagcaaacc attctgggct tgtcaagagc tcaaagcccc attcaatcgc
     8401 ttcatcacgg aaataatcac caatagagaa gttaccaagc atttcaaaca tggtgtggtg
     8461 gcgggctgtc ttaccaacgt tctcgatgtc gttggtacgg attgattttt gggcatttgt
     8521 aatccgtggg ttatctggaa tgacagagcc atcaaagtat ttcttgaggg ttgccacacc
     8581 agagttgatc cacaaaagag tgggatcgtt tacagggaca agatttgctg aaggctcaac
     8641 agagtgaccc tttgatttcc agaaatccaa ccacatttga cgaatttgag cagaagataa
     8701 atttttcata aactaagata ccaagcccga ttagaaggcg actgaaaaat aggaaactga
     8761 caggaaatct tgatttccta ggcagtttat cttttttccg agccttccga gcgggttcaa
     8821 tttaacgaaa cagttaaatt aaacatttcc tttcatatag taataattga ggggaaagtc
     8881 cgtagcccga gtccatttca gaataaactc gatacccttt cctttcataa gtgatagtta
     8941 gacgggttca atttaacgga acagttaaac tgaatgatta tttcacgatt aattcgatgc
     9001 atcttccatc atttcgatgt agtcaattgc gaccaccaag gaaatgacca aatctgcata
     9061 ggagtcctcc aatacagtaa cggaataatg agaagttaaa tgaaagagtt ctttggagat
     9121 ttcagctacg agcataccat ctggcctttt caagcgaaaa tccaaatccc agatatttcc
     9181 gtctaggatt aaacctagat tttccacagt ataacgatcc ttgaaaaaag taaattcctt
     9241 ttgcaagtag aaagaatgtc cctctaccat atcaatggta aactggggca ggaaggtcaa
     9301 aaacttctta gtaatctgac aaacttcccc acctctggaa ttggttacga taaatcgttt
     9361 tggaatttcg agaaaagatc cctccacttg ataagcaaga ttcccaaaac tatcacgaat
     9421 atcaaatttt tcaccaccaa aggtaaagcg ctgtttcact tgaaattgct tcatgataac
     9481 ctccaaaaaa ataacaagaa atcaaacgaa gggcgaaaaa ccgcggtacc accttcattc
     9541 aatgacttgt cattctcatt taagaaactg tatccacaag caaaaaactt ttatatataa
     9601 gccacaaact gtgaatacag gctctactat tcaattgtaa tattaagtag caaaatactt
     9661 ggcttagatg gctcgcaaca accgccaatt ttctgaacta aggtgtcaag taccaattct
     9721 taataagtaa atcgtcaatt actgacctga tgaactagaa gattctgttg tagaagaaga
     9781 actttcagtc gacgaagagc tgctcttcgt tgaggaagaa ctgctttctt ctgttgtcac
     9841 atactgtgaa agaagatttt ggaaagcact gtctttaacc ttaacgtttg ctttttggag
     9901 tgcttctttc agcacagtag caacaaatga tgaatctctt tgcttttgtg ccaaaataat
     9961 ttctttcagc ttatctttat aatcttccca tttagcagat ttttctgatt tagcatccaa
    10021 ttttacaacg taataactcg ttgtatacgt cttcatatct accgaagaaa tcaccgacgc
    10081 tccgacttga cctgcatcca aggcaaatac cgctttctgt acctctgctg gaacagttgt
    10141 tgaagttgaa tcgaatttaa cttcgcctcc attgtctttt gtagtagtat ccgtggaatt
    10201 gtctttcgcc aattgagcaa aatctgcacc ttcagcttgt gcagcagcta aaacttcttt
    10261 tgctttagct tcgtctgcca acttgatgac acgtgctgtt acttcaggtg tataggcgtc
    10321 ataagctgcc ttgtagtttt catctgtcaa ttctttctca gcagcttgtt ttacagcata
    10381 ctcaaccaat ttattggtac gaatttgttc cttataggat tcttctgtca atcctgccga
    10441 agaaagtgcc gcgttaaaag aagcaccaag ttgttctgcc ttcttatcat attccttatt
    10501 tacttcttct gcagagactt tgtcaccata ctgattttca aatacattac tgataaccat
    10561 tgacagcaaa acttgttgag cttgtgagtt tgtttttact ttctcataaa actcagacac
    10621 agtaatcgtg ttacctttca tcgtaatgat atctttatca gctgcattgg aacaagcagc
    10681 gagtgttact gcagcaaaga gtgttactgc acctgcgaga atttttttag tttgtttcat
    10741 taaagactct ctcctttttt cattaacccc tttattataa cacaaaagct taaaaacttc
    10801 ttaaatttct atatgtttta agtcaacatt ctctacattt ttcctcaaca taaggagacc
    10861 gtcactcatg gaaacaagac tagcagtcac atctggatta tccaaagtcg catcgaataa
    10921 acgttgcaat cccttataaa tcgttcgctg ccctcttctg atatccttga tgtccttggc
    10981 aacatctcct ccttggaaga tgtcatccaa aatgattaaa ccacctactt tgaccttttt
    11041 caagacttct ggtaaaaata caatatattt tgatttagca gaatccatga aaataaaatc
    11101 atatgtgtta tctggtaaag taggaagaat atccatagct tctccttcca gcaattcaat
    11161 ctgctggcga taatcatact tggcaaagtt ttccttagca aatccaatca tttcttcatt
    11221 tcgatcaatc gtcgtaatct tcgagtgcgg gctattgtca gccatcagta acgcagagaa
    11281 gccaatagca gttccaactt ctaaaatacg tttaggttgg agtgtctgca tcagcaagcg
    11341 gaaataagct accgtttcat gagggataac tggaatgttt tcttgatgag caaactcttc
    11401 cagttctttc aaaaaccctg tattttgcgc ctgacgtgtt cgcataaaat ccacaatttc
    11461 ttccttgaca accggacggc gcatattgtg atttgcgttt ttactatatg attcaaccat
    11521 agaatccatt atatcataag ccgacaagat tttcttcctc aacacactaa aaggtaggtt
    11581 tccccacctt ttctttacat atttttggcg taatcaattc gataccctaa atttccagct
    11641 agttcttgaa gtcgaataaa agtcgcctgc ttactgaaaa agagatagta actatccgtg
    11701 tccatatcta ctgcccctag acaaaagttt tcatcaagat atttgttatc aatgactctt
    11761 gcccattctg aaatagatgc aattggactt aatccacctg aatccaattt aagagaaatt
    11821 cccgcattgc ggacctgctg aaggcaatca aaaaagtaga taaaatcttc aagattgtcc
    11881 ttataatcac gcacacaaat atagttcaaa tgctctagta attttatcaa gattaaccaa
    11941 agattttcct tttcagactc tccaacacga tccaagaaat catcgaatga ctctgaatct
    12001 aaaatacgtt ccataaaatt gagaatctcg tgtggatcac tggcgaagac tacttctcta
    12061 agttggagaa atagttccct atcaccattt gacattaaat ctgctaattc aaaaatatct
    12121 tgtagttgtt cttctgttaa catagtcccc tctctttatt aatttgaaaa atcccatctt
    12181 atatgcggtg ctacttgctc tacaaaatgg taaatatcat taaaatctgc accatcaagc
    12241 ctattcaagc tgacgactgc aagtagtccc cccttatttc ctacaattcg caaacgtttc
    12301 gactgatagc cacgatagac aggagaaacg atgtatcgaa tctctgcaat ctcttctaac
    12361 gaatggcgtt gacgttcact ccaaaagtgt tcttgaaatt ctaggtactg ttttgtcaag
    12421 ataaaaactg ccttttgctt tctcaatata tataaat
//
LOCUS       DBNFEG010000047        12405 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.047, whole genome shotgun sequence.
ACCESSION   DBNFEG010000047 DBNFEG010000000
VERSION     DBNFEG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12405
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.047"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..310
                     /locus_tag="AC1PNG_000907"
     CDS             <1..310
                     /locus_tag="AC1PNG_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIP6701418.1"
                     /translation="ILRGKGETDSIEIGTILIQPTSGAVYMEEEEVQLTKKEYEVLLY
                     LAKRRGKIVSRDNLMMGIWGYTELDSRVLDNHIKNIRKKLPSLPLKTIVGRGYQIEGT
                     "
     gene            312..1700
                     /locus_tag="AC1PNG_000908"
     CDS             312..1700
                     /locus_tag="AC1PNG_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIP6701419.1"
                     /translation="MGIARKNFIIVAAMISFISVTLLGLLYYAMPIYYNQVKKQELRH
                     DYMTVAKQLDGMPEAKIISEIDDFDIKTPNIIISLFSSDRKIIYPDPNDEMAKKHENE
                     YLENGDFDEIGSWGAEITSAEGVTYYLLFNYGFHSLSDVSQTLVTFYPFILLLIIVLA
                     VSVAFVYSRLSTRRIARISETTRRMQSLESGISCAVAGTDEITILAQDINSLYSKLLS
                     SIDELRTENERAMAREKEKSDFLRITSHELKTPIASMLGLVEGMIYNVGPFKDHDTYL
                     KKCKEILQEQSELVHSILEATNLDMALKESREQIRLDQLIDSSLTSYQSLAEVKGYQF
                     EVELSPVLIEANPVYFLKAIKNILDNAFRYSCKEAIIRVVLRQNQLIIENQVERVLSD
                     EELDQVFRPFYRPDYSRDKKDGGIGIGLFIVQKILEKHGFPYTFYASDTQTMRFMIHL
                     QSQSTANSNELI"
     gene            2142..2693
                     /locus_tag="AC1PNG_000909"
     CDS             2142..2693
                     /locus_tag="AC1PNG_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HIP6701420.1"
                     /translation="MIVFLVICIFLVRIYFLKISIKNEKRILKNGGSEFGVQNTKLLT
                     VVHILFYLSCLVEAVVRHTKFDTLSSLGLIFLLFSMGMLFWVTRLLGDIWTVKLMLVK
                     DHRFVDHWLFRVVKHPNYFLNIIPELIGLALLCHATYSFLILFPVYMVILYRRIHEEN
                     NLLKTVIIPNGTVQSAAEITGNN"
     gene            complement(2902..3810)
                     /gene="ypfJ"
                     /locus_tag="AC1PNG_000910"
     CDS             complement(2902..3810)
                     /gene="ypfJ"
                     /locus_tag="AC1PNG_000910"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_02809 family neutral zinc metallopeptidase"
                     /protein_id="HIP6701421.1"
                     /translation="MKIDDLRKSSNIEDRRGQSSSNSSFSSGSSGGNVLLGPIFSRLG
                     WKGKLLVLVLLVTFGGMSNLGGLLSPSTGTNPYQSSQVTTTGTDVSDADAEFMSKVLG
                     STEDFWVQEFKANGLTYNAPTMVFYTNQTQTGCGVGSAQAGPFYCSADRKIYIDTSFY
                     QELSSKYKAAGDFAMAYVIAHEVGHHVQNELEVLGAYHQKRAQLSDKEGNALNVRLEL
                     QADYYAGAWAKYVDGQGILDAGDIDEAMNAAHAVGDDTLQQEAYGRTVPDSFTHGTSE
                     QRKKWFNLGYTYGDLAHANTFSVEKP"
     gene            3978..5321
                     /gene="xseA"
                     /locus_tag="AC1PNG_000911"
     CDS             3978..5321
                     /gene="xseA"
                     /locus_tag="AC1PNG_000911"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIP6701422.1"
                     /translation="MVEYLSVSHLTKYLKLKFDRDPYLEKVYLTGQVSNFRKRPSHQY
                     FSLKDDKAVIQATMWAGVYKNLGFELEEGMKINVIGRIQLYEPNGSYSIVIEKAEPDG
                     IGALAIKFEQLKQALTQEGLFKEEFKQALPRFTKKIGVITSPSGAVIQDIITTVSRRF
                     PGVEIVLYPTKVQGDGAAQEVVTNIQRANERDDLDVLIVGRGGGSIEDLWAFNEEIVV
                     RAIFESRIPIISSVGHETDTTLADFVADKRAATPTAAAELATPVTKADLLGYLNQQEN
                     RAYQAMTNRLRFLRGQLEKISQSVMFRQPERLYDGYLQKLDRLTNQLQTRMKELYSQQ
                     KQACLLLQRRLQGVHPGRKVESFQERIIQQERLLKSNMANIYDNKMAKADKLIEALTM
                     LDTSRIVARGYAMIRQDGRVIDSVENVQMGENLTIQMKDGQLDVEVKHVQTDENI"
     gene            5296..5511
                     /locus_tag="AC1PNG_000912"
     CDS             5296..5511
                     /locus_tag="AC1PNG_000912"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIP6701423.1"
                     /translation="MSKQTKTFEENLAELEGIVTKLERGDVALEEALAEFQKGMVLSK
                     DLQKTLAEAEKTLVKVMQADGSEAEMD"
     gene            5511..6380
                     /locus_tag="AC1PNG_000913"
     CDS             5511..6380
                     /locus_tag="AC1PNG_000913"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIP6701424.1"
                     /translation="MVASKLQRLEEAMADFYQPQVAERLNQSVLYSLQAGGKRIRPLL
                     LLTILESFGMELKQAHYQVAACVELIHTGSLIHDDLPAMDNDDYRRGRLTNHKVYDEA
                     TAILAGDSLFLDPFGFLAQVDLPAETILALVRELSQASGTFGMVAGQVLDMAGEGQNL
                     TLEQLQAIHANKTGKLLTFPFVAAGLIAGQSDVVINHLRQAGQLIGLAFQVRDDILDV
                     TTTFEEIGKTPQKDVLAEKSTYPALMGLEASKVFLEDSLNQAINELDQVAKQVPFSTE
                     KIKEIIESLRING"
     gene            6373..7194
                     /locus_tag="AC1PNG_000914"
     CDS             6373..7194
                     /locus_tag="AC1PNG_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HIP6701425.1"
                     /translation="MAKERVDVLAFKQGLFETREQAKRGVMAGLVISVINGERYDKPG
                     EKIDEGIELKLKGEKLKYVSRGGLKLEKALQVFHLSVEGQTTIDIGASTGGFTDVMLQ
                     AGAKQVYAVDVGTNQLAWKLRQDERVISMEQYNFRYAELGDFELGQPSFASIDVSFIS
                     LSLILPALHRILAEGGQVVALVKPQFEAGREQIGKNGIVKDKSVHLKVLEEVTKMAVE
                     TGFSVKALSFSPVQGGHGNIEFLAHLEKSTQPNQIDKEVITTTVYQAHEEFKKDE"
     gene            7187..7612
                     /locus_tag="AC1PNG_000915"
     CDS             7187..7612
                     /locus_tag="AC1PNG_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIP6701426.1"
                     /translation="MNKRERLEVIKDLVVRFPIDTQEEIVERLEAMGVNATQATVSRD
                     IKELGIIKVPSADKGYIYGLPKAGLVKVKSQNVFEVSVMDKMINLRLVPGSSAVVKRQ
                     ILEQFQEHVFSVIADDDSILLIVTNEGIISQIEQTVRGW"
     gene            7615..9276
                     /gene="recN"
                     /locus_tag="AC1PNG_000916"
     CDS             7615..9276
                     /gene="recN"
                     /locus_tag="AC1PNG_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIP6701427.1"
                     /translation="MLLEVSIKNFAIIEQVSLNFENGMTILSGETGAGKSIIIDAMNL
                     MLGARATTDVIRHGAAKAEIEGLFSFENSRALEQILLEQGIEVADELIIRREILQNGR
                     SVSRVNGQMVNLSVLKQIGQYLVDIHGQHDQEELMKSQHHIRLLDSFGEDEFWSLKDR
                     YQTTFDAYRSLRKRVLEKQKNEQEHKARIEMLEYQIAEIEAADLKSGEDIQLNQERDK
                     LLNHKQIADTLTNAYALLDNEDFSSLNNLRSAMSDLQSLEEFDPDYKQLSSSLTEAYY
                     VVEDITKRLSDVVDNLDFDGNRLMQLESRLDLLNTITKKYGGTVDDVLDYFSKISEEY
                     NLLTGNDLSGDDLEVQLKNLEKELVERAGQLSQSRHELAVVLEDIIRQELQDLYMEKA
                     RFQVRFTKGKFNREGNETVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFARKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIYKIGQYGQVLAISHLPQVIAIADYQFFIEKIS
                     DEHSTVSRVRLLTKEERIEEIAKMLAGDKITDAARNQAKELVEKG"
     gene            9370..10203
                     /locus_tag="AC1PNG_000917"
     CDS             9370..10203
                     /locus_tag="AC1PNG_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIP6701428.1"
                     /translation="MSKIKIVTDSSTTIEPSLVEELNITVVPLSVMVDGVVYSDNDLK
                     EGEFLELMRGSKNLPKTSQPPVGLFAETFADLAKDGSQIIAILLSHALSGTVEAARQG
                     ATLSGADVTILDSSFTDQAEKFQVVEAARMAQAGASKEEILAAIETVKEKTELYIGVS
                     TLENLVKGGRIGRVQGMLSSLLNIRVIMEMKDHQLTPIVKGRGNKTFKKWLEEFTATL
                     ADKKVAEIGISYSGTADFANEMKSQLQEYVSKEISVLETGSIIQTHTGEDAWAILIRY
                     E"
     gene            10196..11029
                     /locus_tag="AC1PNG_000918"
     CDS             10196..11029
                     /locus_tag="AC1PNG_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIP6701429.1"
                     /translation="MNNRKLIQGFVFFFLAILGSIFLFHQLIPVASSRISRQELTVSE
                     KISFRYVALGDSLTEGVGDTTGQGGFVPLLAQSLTNDYGYEVDYKNFGVSGNTSNQIL
                     KRMKEDGELISYLKTADLLTLTVGGNDLRKAIIKNIANLKVSTFDKPAKDYGKRLDTI
                     IKTARKNNPNLPIYVVGIYNPLYLNFPELTEMQTIVDNWNTMTEQITEKYQHVYFVPI
                     NDLLYKGLEGEAGISQNGSQVTNNLLYEEDSFHPNNTGYEIMKKAILERMNETTESWE
                     P"
     gene            11001..11597
                     /locus_tag="AC1PNG_000919"
     CDS             11001..11597
                     /locus_tag="AC1PNG_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HIP6701430.1"
                     /translation="MRPRKAGNLNIWKWLFLAQLALLIGCGIVLYTRIQTDREDLTKL
                     VQTSTEDTKVGTFSTNREQLNQTLTNYLKEYQTEEFSYQLFVTSQQVVFEGSYQIFGV
                     TIPLYIYFQPSKMEDGSILLRITEISVGSLSLPKAEVLAYLQKNYKLPAFVKIDSEQA
                     QVQVQLTELKNKFGLYGKANTIDLYNDQFIVDIYRKRQ"
     gene            11687..11962
                     /locus_tag="AC1PNG_000920"
     CDS             11687..11962
                     /locus_tag="AC1PNG_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIP6701431.1"
                     /translation="MANKQDLIAKVAEATSLTKKDSAAAVDAVFGAVADYLAAGEKVQ
                     LIGFGNFEVRERSARTGRNPQTGAEIKIAASKVPAFKAGKALKDAVK"
     gene            complement(12055..>12405)
                     /locus_tag="AC1PNG_000921"
     CDS             complement(12055..>12405)
                     /locus_tag="AC1PNG_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIP6701432.1"
                     /translation="LSFSEELRQHYELYQLLLFHFQEKNSDHFFDIVELEIATVNPIF
                     QTVFKTFLKDKDKVLNALELPYSNAKLEATNNLIKVIKRNAFGFRNFENFKKRILIAL
                     NIKKERTKFVLSRC"
BASE COUNT         3908 a         2201 c         2745 g         3551 t
ORIGIN      
        1 tattttgaga ggaaaaggag agacagatag cattgagata ggaacaattc ttattcaacc
       61 aactagcggg gcagtttata tggaagaaga agaagttcaa ttgacgaaaa aagaatatga
      121 agttttacta tatttagcta aacgacgtgg gaaaattgtt agtcgtgata acttgatgat
      181 gggaatatgg ggatatacgg aattggatag tcgtgtctta gataatcata tcaagaatat
      241 ccgtaagaaa ttgccgtcac ttcctttgaa gacaatagtt ggtcgtggtt atcaaataga
      301 gggtacttaa tatggggatt gccagaaaaa attttattat tgttgctgcg atgatttctt
      361 tcatctctgt aacattgtta gggttattgt attatgctat gcctatatat tataatcagg
      421 ttaagaaaca ggaattgaga cacgattata tgactgtggc taaacaattg gatgggatgc
      481 cagaagctaa gattatatct gagattgatg atttcgacat aaaaactccg aatattatta
      541 ttagtctttt ttcgagtgat agaaaaatta tttatccaga ccccaatgat gaaatggcta
      601 aaaagcatga gaacgagtat ttagaaaatg gggattttga tgaaattggt tcttggggag
      661 ccgagataac atctgcagaa ggagttacat attatctgtt atttaattat ggatttcact
      721 cgttatctga tgtcagtcaa acattggtaa ctttttatcc attcatattg ctgttgatta
      781 ttgttctggc agtttcggta gcttttgttt acagtagact gtccactcgt agaatagcaa
      841 gaatttcaga aacaacaaga cgaatgcaat cgttagagtc tggaatcagt tgtgctgttg
      901 caggaactga tgaaataaca atattggctc aggatattaa cagcctgtat agcaagttat
      961 tgtcaagtat cgatgaatta agaacggaaa atgaacgtgc tatggcacgt gagaaggaga
     1021 agtctgattt cctccgtatc acatcacacg aattaaaaac tccaatagct agtatgttgg
     1081 ggttagtcga aggcatgatt tataatgtgg ggccatttaa ggaccatgat acatatttaa
     1141 aaaaatgcaa ggaaatcttg caggaacaat ctgagttagt tcattctatt ttagaagcga
     1201 caaatcttga tatggcttta aaggaaagca gagaacaaat ccgattagat cagttgattg
     1261 attcctcttt aacaagttat cagagtttgg cagaggttaa gggctatcaa tttgaagtag
     1321 aactatcacc ggttctcatt gaggcaaatc ctgtttattt cttaaaagct attaagaata
     1381 ttttagataa tgcctttcgg tactcatgta aagaggctat tattcgtgta gtactacgcc
     1441 aaaatcaatt aataatcgaa aatcaggttg aacgtgtttt aagtgacgag gaattggatc
     1501 aagtctttcg tccattctat cgtccagact acagtcgtga taagaaagat ggtggcatag
     1561 ggattggttt gtttattgta cagaaaatat tagaaaaaca cggctttccg tacacttttt
     1621 atgcttctga tacacaaact atgcgtttta tgattcattt gcaatcacaa agtacagcga
     1681 atagtaatga attgatatag gtaaaatata gacaatattt agtttttctt ttattacttc
     1741 gacgtaagag gaaactccgt ttccttattt ccaacttcaa acactccact ggattgtttg
     1801 aactcgcttt gctgtactct gcaggggtga cttgctccgc aagtgctatc tccaaccttg
     1861 cacagtcact cgactgtaca agctgtctgc agctattgaa gcgaacttgc ttcgccctat
     1921 ctccaacctc caaaggttcc ccaaaccttt ggagcgagta ctaaaagtaa actaaaagac
     1981 tataaaagag acatgatttc atccattttt gaaaacaggt ctcttttatt gtagtctcaa
     2041 atggaaaata gaaaaaaaac aggagaggaa tagaatggcg acagtctaac tttgtataat
     2101 ggatgtgttt gataaaatct gttaaaatac aggagggatt tatgattgtt tttttggtta
     2161 tttgtatttt tttggtgaga atctattttt tgaagatttc aattaaaaac gaaaaacgga
     2221 ttttgaaaaa cggcggttca gaatttggtg ttcaaaatac taaattatta acagttgtac
     2281 atattctatt ttatttatca tgtttggttg aagcagtagt gcgacacacc aagtttgata
     2341 cgcttagtag cttaggtttg atattcttgc tgttttcaat gggaatgtta ttttgggtaa
     2401 cacgattgtt aggtgatatt tggacagtta agctcatgtt ggtaaaagat catcgttttg
     2461 ttgatcattg gttgtttcgt gttgtaaagc atcctaacta tttcctaaat attataccag
     2521 aattaatcgg tttagcttta ttgtgtcatg caacatatag ttttctcatc ttattcccag
     2581 tctacatggt tatcttatac agacgtattc acgaggaaaa taatttgttg aaaacagtga
     2641 ttattccaaa cggaactgtg cagtctgcag cagagataac tggaaataat taaagtttgc
     2701 taaataataa ttgtgatatt gtttgaaact aggataccgc tctaattaga gttgatgagt
     2761 aagtttacat gattcagttt tcatatttaa aagtggtacc gtatgtgtaa attggctttt
     2821 gtcagttgta tattataaag aaactcttgc aaaagtgtat gttgcaagag tttctttatt
     2881 gctacgagag gctatggatg tttacggttt ctctacggag aatgtattgg cgtgagcaag
     2941 gtcaccatag gtataaccga gattaaacca ttttttcctt tgctcagaag tgccgtgggt
     3001 aaaactgtct ggaacagtac ggccgtaggc ttcctgttga agagtatcgt caccaactgc
     3061 atgggcagca ttcatggctt cgtcaatatc accagcatcc aagatgcctt gtccatcaac
     3121 atatttggcc caggctcctg cataatagtc agcttgcaat tctaaacgaa catttagcgc
     3181 attgccttcc ttatcggata gttgagcccg tttttgatgg taggccccaa gaacttctaa
     3241 ttcattctga acatggtgac caacttcatg agcaatgaca taggccatgg caaagtcacc
     3301 agcagcctta tacttgctag ataattcttg atagaaagaa gtatcaatat aaatcttgcg
     3361 gtcagcagag caatagaatg gaccggcttg ggcggaacca actccacatc cggtttgggt
     3421 ctggttagta tagaaaacca tagtcggtgc attgtaggtt aaaccattgg ctttaaattc
     3481 ttggacccaa aaatcttctg tcgatcctaa aaccttactc atgaattcag catctgcatc
     3541 agacacatct gtaccagtcg ttgtgacttg actcgattgg taaggattag tccctgttga
     3601 tggggaaagc aacccaccaa ggtttgacat gcctccgaag gttactaaaa ggacaagaac
     3661 aagtagtttt cctttccaac caagtcgtga aaagattggt cccaatagaa catttccgcc
     3721 agatgaacca gacgagaaag atgagttaga actggattgt cccctgcggt cctcaatgtt
     3781 agaacttttt cgcaaatcat caattttcat ataaatctcc ttagaacctg tcaacaagtt
     3841 cttattttct cacaaactat tatatagcaa attgttgaaa aaaaacatag gaaagctatc
     3901 ggaaataata ggcctagttc tgtataaaag tgcaaaattt ggtataatag tagggataca
     3961 atatagaagg agtggacatg gttgaatatt tatcggttag tcatctaact aagtatttaa
     4021 aattaaagtt tgaccgcgat ccgtatttgg agaaggtgta tttgacaggt caagtgtcca
     4081 acttccgtaa acgccctagc catcaatatt tttcgctcaa ggatgacaag gcagtcatcc
     4141 aagcgaccat gtgggcaggc gtctataaga atctgggctt tgagcttgaa gagggaatga
     4201 aaatcaatgt cattgggcgc attcagctct atgaaccaaa tggttcttat tctattgtca
     4261 ttgaaaaagc tgagccagat ggtattggag ccttggcaat caagtttgaa cagctcaaac
     4321 aagctctgac acaggaaggc ttatttaaag aagaattcaa gcaggccttg ccacggttta
     4381 ccaagaaaat cggtgtcatt accagtccga gtggagctgt tattcaggat attattacaa
     4441 ccgttagccg acgttttcca ggcgtagaga ttgttctcta tccgaccaag gtgcaaggag
     4501 atggtgctgc tcaggaggtt gttactaata ttcaaagagc caatgaacgg gatgacttgg
     4561 atgttctgat tgttggtcgt ggcggtggtt ctatcgaaga cctttgggct ttcaatgaag
     4621 aaattgtggt tcgggctatt tttgaatccc gtattccaat catttccagc gtaggtcatg
     4681 aaacagatac gaccttggct gattttgtag cagataaacg agctgctacc ccaacagctg
     4741 ccgcagaatt agcaacacct gtgaccaagg cggacttact gggctacctc aatcagcagg
     4801 aaaatagagc ctatcaggcc atgaccaatc gtttgagatt cctaagaggt cagcttgaga
     4861 aaattagcca gtcagtcatg ttccgccagc ctgaacgctt gtatgatggt tatttgcaaa
     4921 aattagaccg cttgaccaat caattgcaga cacggatgaa agaactctac agccagcaga
     4981 aacaggcttg tctcctacta cagcgacgat tacaaggtgt gcatccaggt cgtaaagtag
     5041 aaagtttcca agagagaatt atccagcagg aacgactctt gaagagcaat atggctaata
     5101 tctatgacaa taagatggcc aaggcggaca agctgattga ggccctgact atgctggata
     5161 ccagtcgcat cgtagcccgt ggctacgcca tgattcgtca agatggtcgg gtgatcgata
     5221 gtgtagaaaa tgtacaaatg ggtgaaaacc taaccatcca gatgaaggat ggtcaacttg
     5281 atgtggaggt aaaacatgtc caaacagacg aaaacatttg aagaaaactt agctgaatta
     5341 gaaggaatcg taaccaaatt ggagcgaggc gatgtggctc tggaagaagc actggccgag
     5401 tttcaaaaag gaatggttct atcgaaagac ctacaaaaga cactagcaga agctgaaaag
     5461 accttggtca aggtcatgca ggcggatggc agtgaagcgg agatggatta atggttgcca
     5521 gtaaattgca gcgacttgag gaagccatgg cagactttta ccaaccccag gtagctgaac
     5581 gcctcaacca atcggttctt tattcgctac aggctggtgg taaacgaatc cgccccttgc
     5641 ttttattgac catcttagag tcctttggga tggaattaaa acaagctcat taccaagtgg
     5701 cagcatgtgt cgagttgatc catacgggaa gtttgattca cgatgacctg cctgctatgg
     5761 ataatgatga ttaccgtcgt ggtcgcttga ccaaccacaa ggtttacgat gaagcaacgg
     5821 ctattttagc aggggacagt ctctttctag atccatttgg tttcctagcc caagttgatt
     5881 taccagctga gaccatcctt gctctcgtga gagagctttc ccaggcatct gggacctttg
     5941 gaatggttgc tggtcaagtc ttggatatgg ctggtgaagg gcagaatttg accttagaac
     6001 aattacaagc catccatgcc aataagactg gaaaattatt gacatttccc tttgtagcag
     6061 caggtttgat tgcaggacaa tcagatgtgg taatcaacca tttgcgacaa gcgggtcaat
     6121 taattggtct agcctttcaa gttcgggatg atattttgga tgtgacaacc acttttgagg
     6181 aaatcggaaa aactccccaa aaagatgtat tggctgagaa gtctacctac cctgccctaa
     6241 tgggactgga agcctcaaaa gtctttctag aagatagctt gaatcaagca atcaacgagc
     6301 tagatcaagt ggctaaacaa gtcccatttt caacagaaaa aatcaaagaa atcatagaaa
     6361 gtttacgaat caatggctaa ggaaagagta gatgtactag cttttaaaca ggggcttttc
     6421 gagacgcggg agcaggccaa acgcggtgtt atggctggct tagttatatc cgtcattaac
     6481 ggggaacgct acgataaacc aggtgagaag attgacgagg gaattgaact caagctcaag
     6541 ggggaaaaac tcaagtatgt cagccgtggt ggtctcaaat tggagaaagc cttgcaggtt
     6601 ttccacttat cagttgaggg tcaaacaacg attgatattg gggcttcaac aggcggattt
     6661 acggatgtta tgttgcaggc tggtgccaag caggtctatg ctgtggatgt agggaccaac
     6721 caactcgctt ggaaattacg tcaggacgaa cgtgtaatca gcatggaaca gtacaatttc
     6781 cgctatgctg agttgggaga tttcgaactt ggtcaaccaa gttttgcgtc cattgacgtc
     6841 agctttatct cattgagtct gattttgcca gcactccatc gtattttagc ggagggtggc
     6901 caggttgttg cccttgtcaa accccaattc gaggctggac gtgaacagat tgggaaaaat
     6961 ggcattgtta aggataaatc cgttcacttg aaagtcttgg aagaagtgac aaaaatggcc
     7021 gtggaaacag gcttctcagt caaagccctt agcttttccc ctgttcaagg cggtcatggc
     7081 aatatcgaat ttttagcgca cttagaaaaa tcaacacaac caaaccagat tgataaagag
     7141 gtcataacaa ctacagtcta tcaagcacat gaggaattta aaaaggatga ataaacgaga
     7201 acgattagaa gtcattaaag atttggtagt ccgatttcct attgatacac aggaagaaat
     7261 tgttgaacgt ctagaagcaa tgggtgtcaa tgcgacacaa gcaacggttt cacgtgatat
     7321 caaggaactt ggcattatta aggttccatc agcagataag ggatatattt atggtttacc
     7381 aaaggcaggt cttgttaagg taaaatcgca gaatgttttt gaagtctcag tgatggataa
     7441 aatgattaat ctccgtctgg tacctggaag ctctgctgtg gtcaaacgcc agattttgga
     7501 acaatttcaa gaacatgttt tttcagtgat tgctgatgat gacagtattc tgctcattgt
     7561 tacaaacgaa ggtattattt cgcaaatcga acagacggtt agagggtggt agccatgtta
     7621 ctagaagtgt ccattaaaaa cttcgccatt attgagcagg tatcactgaa ttttgaaaac
     7681 gggatgacca tcctatctgg tgaaacaggt gcaggtaaat ccattatcat tgatgccatg
     7741 aacctgatgc taggggcgcg tgcgacgaca gatgttatca gacatggggc cgccaaggct
     7801 gagattgaag ggctcttttc ctttgaaaat agcagagctc tcgagcaaat tttgcttgaa
     7861 caggggattg aagtagctga cgaactcatt atccgccgtg aaatcctgca aaatggccgt
     7921 tcggtcagcc gtgtcaatgg acaaatggtc aatttatctg tcttgaaaca gattgggcaa
     7981 tatttagtag atattcatgg tcaacatgac caggaagaat tgatgaaatc ccagcaccat
     8041 atccgtcttt tggacagttt cggagaagat gaattttgga gtcttaaaga ccgttatcag
     8101 acaacctttg atgcctatcg tagtcttcgc aaacgagttc ttgaaaagca aaaaaatgag
     8161 caagaacaca aggcgcggat tgagatgcta gaataccaaa ttgctgaaat cgaagcagcg
     8221 gatttgaagt ctggggaaga tattcaactc aatcaggaac gcgataaact gctcaaccac
     8281 aaacaaattg cagatacact gaccaatgcc tatgcactgt tagacaatga agatttttca
     8341 agcttgaaca acctacgctc agctatgagt gacttgcaaa gtctggaaga atttgatcca
     8401 gactacaaac agctctcttc tagcctgaca gaagcttatt atgtcgttga agatataacc
     8461 aagcgtctca gcgatgtggt cgataatcta gattttgacg gcaatcgtct catgcaattg
     8521 gaaagtcgtc tggatttgct caataccatt acgaagaaat atggtggaac tgtcgatgat
     8581 gttttggact attttagtaa aatcagcgaa gaatacaatc tattgacagg caatgattta
     8641 tctggagatg atttggaagt tcagcttaag aacttagaga aagaattggt tgaacgagca
     8701 ggtcagctca gccaatcacg ccatgaattg gcagttgttt tagaagatat tattcgccaa
     8761 gaattgcaag acttgtatat ggagaaagct cgtttccaag ttcgatttac taaaggaaaa
     8821 tttaatcgag aaggaaatga aacagtagaa ttttacattt ctaccaaccc aggtgaagac
     8881 ttcaaaccac tggttaaggt agcttctgga ggagaattgt ctcgcttgat gttggccatt
     8941 aagtcagcct ttgcccgtaa agaaggtaaa acttctattg tctttgatga ggtggataca
     9001 ggggtgtctg gacgtgtggc ccaggcaatt gcccagaaaa tttataaaat cggacaatat
     9061 ggtcaagtat tggcgatttc tcatctacca caagttattg ctattgcaga ttatcagttc
     9121 tttattgaga aaatatccga cgaacattcg actgtttctc gtgtccgtct cttgaccaaa
     9181 gaagagcgta ttgaggaaat tgccaagatg ttagccggtg acaaaatcac agatgcagcc
     9241 cgtaatcaag caaaagaatt agtagaaaaa ggttaggcga ctagcctttt tgcttatgtc
     9301 caaagtttat tttttagtta aaatttgtta taatagatgg caaaattgaa gagaattgta
     9361 ggatgaaata tgtcaaagat taagattgtt acggattcaa gtacgactat tgaacccagt
     9421 ttggtcgaag aattgaatat aacagttgtt ccattatctg taatggttga cggagtcgta
     9481 tactctgaca acgatttaaa agaaggagaa ttcttagagc tcatgcgtgg gtcgaaaaac
     9541 ttgccaaaaa caagtcagcc cccagtagga ctctttgcgg aaacttttgc tgatttggct
     9601 aaagatggca gtcagattat tgctattctc ttgtcgcatg ccttgtcagg gacagtagaa
     9661 gctgctagac aaggagcaac cctatcaggt gctgatgtga caatactcga ttcaagcttt
     9721 acagatcagg ctgaaaaatt ccaagttgta gaagcagcac gcatggcaca agctggtgcc
     9781 agcaaagaag aaatccttgc agctattgaa acagtcaaag aaaaaacgga attatacatc
     9841 ggtgtatcaa ccctagaaaa tctggtcaag ggtggtcgta ttggacgtgt tcaagggatg
     9901 ctgagtagcc tactcaatat ccgtgtcatc atggaaatga aagaccatca gctgactccg
     9961 attgtcaaag gacgaggtaa taagaccttt aaaaaatggc tggaagagtt cacggcaact
    10021 ctagcagata aaaaagtagc cgagattggt atttcctatt caggaacggc tgattttgca
    10081 aatgaaatga agagtcaatt acaagagtac gtctcaaaag aaatttctgt tttagagaca
    10141 ggttccatca ttcaaactca tactggtgag gatgcctggg ctattttgat tcgttatgaa
    10201 taacaggaag ttgattcaag gctttgtttt cttttttctg gcaattctag ggtcgatttt
    10261 tctatttcat caactcattc ctgttgcctc atctcgcatc tccagacaag agcttaccgt
    10321 atcagaaaaa atcagttttc gatatgtggc acttggtgat tctttgacag aaggtgttgg
    10381 agatacaact ggacaggggg gctttgttcc cttgttggct caatctctaa ctaacgatta
    10441 tggttatgaa gtagattata aaaatttcgg agtttctggg aataccagta atcagattct
    10501 taagcgaatg aaggaagatg gagagctgat aagttactta aaaacagcag atctgttaac
    10561 tctgacagtt ggtggcaatg atttgcgcaa ggctattatt aaaaacattg ccaatctcaa
    10621 agtttcaact tttgataagc ccgcaaaaga ttacggaaaa cgattggata caattattaa
    10681 gacagcgcgt aagaataatc caaatcttcc catctatgtt gtcgggattt acaatccact
    10741 ctacctcaat tttcctgaat tgacagaaat gcagacaatt gttgataatt ggaatacgat
    10801 gacagaacag ataactgaaa aatatcaaca tgtctacttt gttccaatca atgatttgct
    10861 ctataaagga ttggaagggg aggctggtat tagtcaaaat ggtagccaag ttaccaataa
    10921 tttactgtat gaggaagaca gtttccatcc caataatact ggatatgaga ttatgaaaaa
    10981 agcgatattg gagagaatga atgagaccac ggaaagctgg gaaccttaat atttggaaat
    11041 ggctattttt agcccagttg gctttattga ttggttgcgg aatagtcctc tatacacgga
    11101 ttcaaacaga tagagaggat ttaacaaagc tcgttcagac tagtacagaa gacacaaagg
    11161 ttgggacctt ctctactaat agggaacagc tcaaccaaac cctcactaac tatctaaaag
    11221 agtatcagac ggaagagttt tcctaccaat tattcgtaac gagtcagcaa gttgtttttg
    11281 aaggcagcta tcagattttt ggtgtgacta tcccactcta tatttatttt caacctagca
    11341 aaatggaaga tggcagtatt ttgttacgga taacagaaat atcagttgga agtttatcct
    11401 tgccgaaagc agaggtatta gcttatttac aaaaaaatta taaactacct gcctttgtaa
    11461 aaatagactc agaacaagcg caagtacagg ttcaactgac agaactgaag aacaaatttg
    11521 gtctgtatgg aaaggcgaat acgattgatt tgtacaacga ccaattcatt gttgatatct
    11581 acagaaaaag acagtaaaat agcattaaaa acttgaccaa acgcctaaaa ttcggtacta
    11641 tatagaagtg agtaataact cagaaaatta ttggaggact tagcaaatgg ctaataaaca
    11701 agatttgatt gcaaaagttg cagaagcaac ttcattgact aaaaaagact cagcagcagc
    11761 tgttgatgca gtattcggag cagtagcaga ctaccttgca gctggtgaaa aagtacaatt
    11821 gatcggtttc ggtaactttg aagttcgtga gcgttcagca cgtacaggtc gcaaccctca
    11881 aacaggtgca gaaatcaaaa tcgcagcatc aaaagttcca gcattcaaag ctggtaaagc
    11941 tcttaaagac gctgttaaat aattcagcat taaaaagccc gtcatatcaa gctttctagc
    12001 ttgtccgacg ggcttttttc ggctctttgt caactgtagt gggtagataa aaagctaaca
    12061 cctagagagg acgaacttcg ttctctcttt ctttatgttc aaagcaatca aaatgcgttt
    12121 tttaaagttc tcaaagttcc tgaaaccaaa ggcatttctc ttaatgactt tgatgagatt
    12181 attagtagct tccagtttgg cgttggaata aggcaattct agggcattca aaaccttgtc
    12241 tttatccttt agaaatgtct taaataccgt ctggaaaata ggattaacag tggctatttc
    12301 cagttcgacg atgtcaaaga aatggtctga gttcttctct tggaaatgga ataagagcaa
    12361 ttgatagagt tcatagtgct gtcgtagttc ctcggagaaa gacag
//
LOCUS       DBNFEG010000048        12063 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.048, whole genome shotgun sequence.
ACCESSION   DBNFEG010000048 DBNFEG010000000
VERSION     DBNFEG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12063
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.048"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(196..4455)
                     /locus_tag="AC1PNG_000922"
     CDS             complement(196..4455)
                     /locus_tag="AC1PNG_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HIP6701433.1"
                     /translation="MKKETSKFTKKVILGQLLICTSFLVWAGQTVAAEEVSQGEPVAI
                     NNETPPAIEAKPLVEATPSTETEPSTPEEKQEEPQVADKNVVEGKKPVTNSQHQMVSE
                     LSTVPSVSIKNPDAATDGSAYGSSDDANSNTKIIAGKETGESDNGYSKWDHVYVQYDF
                     EKEVPVHTVKIHRNTYDNAVSTFKDVKVELSTSVDFSPETTEVIFKQADVEETIANKG
                     QPQVIQLPAPINARYIRIWGRGHYIQNTNSSWKGYSNGVLFNEIEVLAQVEADAGTNE
                     EPQTELVNLAKLKKPYFYGKAPTNPEAINDGKFDENYTTQESIGNQAYLQYEFRNVYT
                     IEEIKVQLAPGEYRNFRIDLDDDYNDGNNYNTIYKNENFTIDKEQIITIKTPKNSKAR
                     YVRFRGTRIDDTPLRYSEIQIMGRGKSYDESAPEYIPPKSEYDTLVWSDEFNGDKIDE
                     SKWQIIDGMWNHAAIYNRKAVSIKKDGDKSYLSIRSTNHKDKKTLVEAVGHDRYDDKE
                     LPDKVTWSSGRLESKDKYSFQFGRMAVRAKVNDSKGIWPAIWMLAQDETGHDEIDVLE
                     YLGQNPWDAWTTNHFGILAKNKKSDGSAYKNYEAWSQEFHVYEVEWTPDVIKWYIDGK
                     FVFQTDRGKDRDGMHTRPMFPILETQVGDGWVGDVDYKRNMTKQDSEYLVDWVRIYQK
                     ADQSQVHFDNLDDAKPNDYVIKPQQKLGHLVTVSNGKAAHENKNNFYYGGQPRYETSR
                     LYADGDGENALVYKIANPEALHLTTYYKTLEDYSVYNKEAGANEGKSVRKHLINAKDG
                     EIDFTVYSSVDGKSWNKENVTVVDNFVEATPAYARTTFDIRNIRKGTKFVKIVFPTVK
                     GISYKLEDGTEKGLQASDVQLAKVTFVATHPEAEPEKPIVDQEKLALSKVFSLDAGRK
                     YFSVNQIKEIIDLISSYGYTDMNLLLGNDGLRFLLDDMNLTVGDKVYDSEAVRKAIIN
                     GNKHYYDDPNGTTLSQSEMDDILAYAKEKNISIIPTINSPGHMDAIVEAMEELGIKSP
                     KFSYNGKTSARTVDLKNDQAVAFTKALIDKYASYFAGKSKIFNIGLDEYANDATNASG
                     WHILQTQGDYGKFIQYANDLAAIVKKHGLEPMAFNDGIYYNHDTSSGTFDKDIIASFW
                     TGGWGGYDVASSKSLHEKGHKILNTNDAWYYVIGREAEGLGWYNLDQGLRGTKSTRFD
                     QVPKSENTKVPIIGSMVAVWADDPSKPYSYDSIKKLLHSFTENNSDYFLAEYRDLREE
                     VGKIPTDLNSYTPESVATLKEVLNKIDWKLSRNNQAFVDGYLAEVKAAREALVPVTAK
                     GDSVKAESLPEGKLPLITAKGKSVQAEPRPAFKGYLTSNYGPSVTPLSLVSKPSSEQS
                     RSQQAASQKKELPNTGAVDGLGFSLLGMLGLAFASRRRKEG"
     gene            complement(4826..6166)
                     /locus_tag="AC1PNG_000923"
     CDS             complement(4826..6166)
                     /locus_tag="AC1PNG_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIP6701434.1"
                     /translation="MRTSFIQSSHQEACIQAIQELVAFPSVLQEHQADTPFGQAIQDV
                     LEHTLALTEKMGFKTYLDPAGYYGYAEIGQGEELLAILCHLDVVPAGDLSQWQTPPFE
                     AVVEGDYIIGRGVQDDKGPSMAALFAVKALLDAGVQFNKRIRFIFGTDEETLWRCMNR
                     YNQLEEVATMGFAPDSSFPLTYAEKGLLQAKLHGPGHPCLSIEAGTAYNVVPAKASYS
                     GHLLAGVIAELDQLGFDYETKDDQVTVLGISRHAKDAAEGVNAIVRLAKALEHFESHP
                     ALDFIVNAVGEDATGFKLFGDVTDEPSGTLSFNIAGLTISAKKSEIRLDIRIPVTADK
                     EALVSTLQAKAQSCGLTYEEYDYLASLYVPLDSQLVSTLMSVYQDKTGDLTSEPISSG
                     GATFARTMPNCVAFGACFPDTEQTEHQENERMPLEDLYKTMDIYAEAVYRLAAE"
     gene            complement(6285..7787)
                     /locus_tag="AC1PNG_000924"
     CDS             complement(6285..7787)
                     /locus_tag="AC1PNG_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HIP6701435.1"
                     /translation="MSEKVKKGFKLPSSYTILMLIIAFMAVMTWIIPAGQYQVDEAGR
                     LVAGTYEKVAQNPQGIYDVFMAPVRAMLGHGATEAAINVAFFIIMVGAFLGVVNETGA
                     LDVGIASIVKRFKGREKMLIYILMPLFALGGSTYGMGEETMAFFPLLVPVMMAVGFDS
                     ITGVAIILLGSQIGCLASTVNPFATVVASDAAGVSVADGMIWRLVFFVVILLMGVLFV
                     ANYAEKVKNDPTKSLVYKQREADMQHFNVTATQEVNAELSPAQKRVLWVFVLTFVLMI
                     CSFIPWEDLGVTIFTQFKDWLIGLPFIGQVIGSSTAALGTWYFPEGAMLFGIAGIVVG
                     LVYGMSEERLVKSFMFGAADIFSVALICAIARGIQVIMNDGMITATILHMGEEGLQGL
                     SSQVFIILTYLFYLPMSFLIPSSSGLAGASMGIMAPLGEFVNVPASLVITAFQAASGL
                     LNLVAPTSGIVIGALALGRVEIGTWYKFVGKLIVGIMIASIAILVIATFF"
     gene            complement(8130..9077)
                     /gene="arcC"
                     /locus_tag="AC1PNG_000925"
     CDS             complement(8130..9077)
                     /gene="arcC"
                     /locus_tag="AC1PNG_000925"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HIP6701436.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQKEALVQTAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQNLAADSEKNPAFPLDSLVAMTEGSIGFWLQNALENELHKE
                     GIEKDVASVVTQVIVDKNDPAFENLTKPIGPFYTEEEAKAEAEKTGATFKEDAGRGWR
                     KVVASPKPVGIKEIGTIRTLLNAGEVVVAAGGGGIPVVQEADGTLTGVEAVIDKDFAS
                     QCLAELVDADLFIVLTGVDYVFVNYNKPDQEKLETVTVAELEEYIKQNQFAPGSMLPK
                     VEAAIAFVNNKPQSKAVITSLENLGALIESDSGTIIVKG"
     gene            complement(9195..10208)
                     /gene="argF"
                     /locus_tag="AC1PNG_000926"
     CDS             complement(9195..10208)
                     /gene="argF"
                     /locus_tag="AC1PNG_000926"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIP6701437.1"
                     /translation="MTNVFKGRHFLAEKDFTRAELEWLIDFSAHLKDLKKRNIPHRYL
                     EGKNIALLFEKTSTRTRAAFTVASIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQKMVEELAEFSGVPVWNGLTDAWHPTQMLADYLTVKENFGKLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEEEVVALAEVFAKESGARVLITDNA
                     DEAVKGADVLYTDVWVSMGEEDKFAERVALLKPYQVNMELVKKAENENLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGDPFV
                     PRV"
     gene            complement(10227..10667)
                     /locus_tag="AC1PNG_000927"
     CDS             complement(10227..10667)
                     /locus_tag="AC1PNG_000927"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6701438.1"
                     /translation="MPSIERTTIGSEEWQRAASIYVRYKVFVLERGIAKEGEFDQNDT
                     EGRVYANLFIGEEPVSTGRFLPVGPGQARLTRIATLPNFRGKGYGKMIISSLEAYARE
                     AGFHQLNIHSELTAKTFYESVGYQATSEVYQEDGEWCQALTKFI"
     gene            complement(10667..11896)
                     /gene="arcA"
                     /locus_tag="AC1PNG_000928"
     CDS             complement(10667..11896)
                     /gene="arcA"
                     /locus_tag="AC1PNG_000928"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HIP6701439.1"
                     /translation="MSNHPIHVFSEIGKLKKVMLHRPGKEIENLMPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGVEVLYLEKLAAESLVTPEIREQFIDEYLEEANIRGRAT
                     KKAIRKLLLSIKDNQELVEKTMAGIQKAELPEIPAEEKGLTDLVESSYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMYSETRNRETLYGKYIFTHHPEYGGKVPLVYNREETTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFEQHVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLRVFSVTYENDTLHIEEEHGDLAELLAANLGLEKVELIR
                     CGGGDMVAAGREQWNDGSNTLTIAPGVVVVYKRNTITNAILESKGLRLIKIGGSELVR
                     GRGGPRCMSMPFEREDI"
BASE COUNT         3491 a         2690 c         2184 g         3698 t
ORIGIN      
        1 ataagattta ttacggggga tttttagtag cctataaaaa attatcctgt tattcatagc
       61 cttctagttt actttttgta caaggcaatg agctgcttgc tatacttagg tacggcaaag
      121 cgagtgaaag aaaaactaaa tgactgatat gtaattacaa ttagaaagcc ctgaacattc
      181 actcaaatgg ctatattacc cctccttccg ccggcgacta gcaaatgcca gaccaagcat
      241 accaaggaga gagaatccaa gaccatctac tgcgcctgta tttggcaatt ctttcttctg
      301 agaagctgct tgctgggagc gtgactgctc actgcttggt tttgatacga gagataaagg
      361 tgtgacagat ggtccatagt ttgatgtcag gtatccctta aaggcagggc gtggttctgc
      421 ttggacactt tttcctttag cagtaatcag aggtagcttg ccttctggta aggactctgc
      481 tttgacgctg tctccttttg ctgtcacagg aactaacgct tcacgcgctg cttttacttc
      541 tgccaagtaa ccatcaacaa acgcttggtt atttctactt agtttccaat caatcttgtt
      601 caagacctct tttaaagtcg ccacgctctc tggcgtatag gaatttaaat cagtaggaat
      661 cttcccaact tcttctcgca aatctcggta ctctgctaaa aaataatcag aattgttttc
      721 cgtaaagctg tgcaaaagtt ttttaattga gtcatagctg taaggcttgc ttgggtcatc
      781 tgcccatacc gcaaccatgc taccgataat gggtacctta gtattctcag atttcggtac
      841 ttgatcaaac cttgttgatt tagttcctcg aagaccttga tcaaggttat accatcccaa
      901 accttctgct tcacgtccaa taacatagta ccaagcatca tttgtattaa gaattttatg
      961 ccctttttca tgaagggatt ttgaggaagc aacatcatat cctccccaac ctcctgtcca
     1021 gaaagaagca ataatgtctt tatcaaaagt acccgaacta gtatcgtggt tataatagat
     1081 accatcattg aaagccattg gctctaaacc atgcttcttg acgatagctg ctaaatcatt
     1141 ggcatattga ataaacttac cataatcacc ttgtgtctga agaatgtgcc atccagaagc
     1201 atttgtagca tcgttagcat actcatctaa gccaatattg aaaattttag atttgcctgc
     1261 aaagtaacta gcatatttat caattaaggc tttagtaaaa gcaactgctt ggtcattttt
     1321 taaatcaact gtacgtgcag atgttttgcc attataacta aattttggcg attttatccc
     1381 caattcttcc attgcttcta caatcgcatc catatgacct gggctattga tagttggaat
     1441 aatggaaata tttttttcct tagcataagc aagaatatca tccatctcac tctgacttaa
     1501 ggttgtaccg tttggatcgt cataataatg cttattacca tttattattg cttttctaac
     1561 tgcttcactg tcatacacct tatctccaac agtcaaattc atatcatcta ataaaaatct
     1621 cagaccatca tttcccaata agaggttcat atccgtgtaa ccataagaag aaattaaatc
     1681 aataatctcc tttatctgat ttacagagaa atacttgcga ccggcatcga gtgaaaaaac
     1741 tttggacaaa gctagttttt cttgatcaac aataggtttt tctggttctg cttcaggatg
     1801 agtagctaca aaagttactt ttgctaactg gacatcactg gcttgcaggc ctttctctgt
     1861 tccatcttcc aacttatagg aaataccttt gaccgttgga aagacgatct tcacaaattt
     1921 agtacctttt cgaatgttgc gaatatcaaa tgtagtgcgt gcataagctg gggttgcttc
     1981 tacaaaatta tccactacag taacattctc tttattccag cttttgccgt caacagatga
     2041 atacacagtg aagtcaattt caccgtcttt tgcatttatc aaatgtttgc gaacagattt
     2101 tccttcatta gcccctgctt ctttattata gacagaatag tcttcaagtg ttttgtaata
     2161 agtcgttaag tgcaaagcct ctggattagc aatcttgtaa actagtgcat tttcgccatc
     2221 accatcagca tagagcctgc ttgtttcgta acgtggttga cctccatagt agaaattgtt
     2281 tttgttttca tgagctgctt tcccgttaga gactgttacg aggtgaccaa gtttttgctg
     2341 cggcttaata acataatcat taggcttggc atcatctaga ttatcaaaat gtacctgact
     2401 ttgatctgct ttttgataaa ttcgaaccca atcaaccaag tactcactat cctgtttagt
     2461 catgttgcgt ttgtaatcaa catcaccaac ccagccatca ccaacttggg tttccagtat
     2521 tgggaacata gggcgggtgt gcataccatc cctatcctta ccacggtcgg tttgaaaaac
     2581 aaattttcca tcaatatacc atttgatgac atctggagtc cattcgactt cgtagacatg
     2641 gaattcctga ctccaagctt cgtaattctt ataagctgag ccatcagatt ttttattttt
     2701 tgccaaaata ccaaaatggt tagttgtcca tgcatcccat ggattttgac caaggtattc
     2761 caaaacgtca atttcgtcat gcccggtctc atcctgtgcc aacatccaaa tagcaggcca
     2821 gattccctta gagtcattta ccttagctcg aacagccatt cgaccaaatt ggaaagaata
     2881 cttatcttta gattctaggc gaccagaaga ccatgttact ttatctggta attctttatc
     2941 atcataccga tcgtgcccaa ctgcttcaac caaagttttt ttatccttat ggttcgtgct
     3001 acggatagac aaatagctct tgtcgccgtc ttttttgata gaaactgctt tacgattgta
     3061 tatagccgca tgattccaca tgccatcaat aatttgccat ttactttcat caatcttgtc
     3121 gccattaaat tcatcagacc agactaatgt atcgtattca gattttggtg gaatgtattc
     3181 tggagcagat tcgtcataag attttcctcg tcccataatc tgaatttctg aatagcgaag
     3241 aggagtatca tctatgcgcg tacctctaaa gcgaacataa cgcgccttag aattttttgg
     3301 agttttaatc gttatgatct gttccttgtc aatggtgaaa ttttcattct tatagattgt
     3361 attataatta ttaccatcat tataatcatc atccaaatct atccggaaat tacgatactc
     3421 tccaggagct aactgaacct tgatttcctc aatagtatag acatttctaa attcatattg
     3481 aagataggcc tggttcccta ttgattcttg agtagtataa ttctcgtcga actttccatc
     3541 gttaatggct tctggattag ttggagcctt accatagaaa tatggttttt tcaatttagc
     3601 gaggttgact agctcggttt gtggttcttc attcgtacca gcatctgctt caacctgcgc
     3661 caagacttct atctcgttaa agagaacgcc attactatat cccttccatg aactgtttgt
     3721 attttggata taatgccccc gtccccaaat acgaatataa cgagcattga ttggtgctgg
     3781 taattggata acttgaggtt gtcctttatt tgcaattgtt tcttctacat ctgcctgttt
     3841 gaaaatgact tctgtggttt caggagaaaa atctacactt gtagacaatt ccactttaac
     3901 atctttaaaa gtagagactg cattatcata agtattacga tgaattttta cagtatggac
     3961 gggaacttcc ttttcaaaat catactgaac gtaaacatga tcccacttgg agtaaccgtt
     4021 atctgattcg ccagtttctt taccagcaat gattttggta ttgctattgg catcgtcgct
     4081 actaccgtaa gcactcccat cggtagctgc atctggattt tttatagaaa cagaagggac
     4141 cgttgataat tccgaaacca tctgatgctg gctgttagtt acgggctttt tcccttctac
     4201 cacattttta tcagctactt gtggttcctc ctgtttttct tctggagttg aaggttctgt
     4261 ttcagttgat ggagtagctt ccacaagtgg ttttgcttca atagcaggtg gagtttcatt
     4321 gttgattgct actggttccc cctgagatac ttcctctgct gcaacggtct gccctgccca
     4381 taccaaaaaa ctggtacaga tgagaagctg accaaggatg acttttttcg taaacttact
     4441 tgtttctttt ttcattattt cctcctataa agtctgcaag tttatcctac ctatagccaa
     4501 aataaagcag tttttaaacc tatttagtct cgtataagac tcaaaattca gtttactggt
     4561 tgatgtgcga tcgcatttct gacacaaata taacatgtaa gcgttttatt gtagaatagt
     4621 ctaacttgac ttattctata tgcagatggg ttctatttct aaaacagcat ataataagtc
     4681 acactttcat atatttttgt atacttatcg taaatagtga cacatttcta tctcatcttt
     4741 ctaatgatat tcttcataag aaaagccctt agatatttca ctaagagctt tgaaactgta
     4801 atttactatt caatactgat aatggctatt ccgcagctag gcgatagact gcctcagcat
     4861 aaatatccat tgttttatat aagtcttcca atggcatgcg ttcattttcc tgatgctctg
     4921 tttgttctgt atctgggaaa catgcaccaa aggccacaca gtttggcata gtacgagcaa
     4981 aggtagcccc acctgacgag attggctcgc ttgttaaatc tcctgtctta tcttgataaa
     5041 cagacatgag cgtagatacc agctggctgt ctaatggcac atacaaggaa gcaaggtagt
     5101 cgtactcttc ataggtcaag ccacatgatt gcgctttggc ttgtaaagta gaaactaggg
     5161 cttctttatc cgcagtgaca ggaatacgaa tgtccaagcg aatttctgat ttttttgcgc
     5221 taatggttag acctgcaata ttgaaactga gtgtccctga tggttcatcg gtcacatcac
     5281 caaaaagctt aaatcctgtg gcatcttcac caactgcatt gacgataaag tccaaggctg
     5341 gatgactttc gaaatgttcc aaagcctttg ccaaacgaac gatggcattg accccctcgg
     5401 cagcatcctt ggcgtgccgc gaaataccaa gaaccgttac ttgatcatct ttggtttcat
     5461 aatcaaagcc taattgatct aattccgcaa taactccggc taataaatga ccagaatagc
     5521 ttgccttggc aggtactacg ttataggcag ttcccgcttc gatactcaga caagggtgac
     5581 ctggaccatg caatttggcc tgtagcaatc ctttttcagc ataagtcaaa gggaaggaag
     5641 aatctggtgc aaagcccata gttgctactt cttccaactg attgtagcgg ttcatacacc
     5701 gccataaggt ctcttcatct gttccaaaaa tgaaacgaat ccgtttgtta aattggactc
     5761 ccgcatcaag aagtgctttt actgcaaata aggccgccat cgaaggacct ttatcatcct
     5821 gaaccccgcg tccaataata taatctccct ctaccacagc ctcaaaaggc ggcgtctgcc
     5881 attgactaag atcaccagca ggtaccacat ctaagtggca aaggatagct aataattctt
     5941 cgccttgccc aatttcagca tagccgtaat aaccagcagg gtctaaataa gttttaaaac
     6001 ccattttttc cgtcaaagcc aacgtgtgct ccagaacatc ctgaatcgct tgaccaaatg
     6061 gcgtatctgc ctgatgttct tgcaaaacag aaggaaaagc aaccagttct tggatggctt
     6121 gaatacatgc ctcctgatgc gatgactgaa taaaacttgt tctcattgtg tcccctttca
     6181 acataataag atatgatata ttttctagca ctattctatc ataaaaggaa aaactgagcc
     6241 aaattttatc tgtattcgtg ataaaatctg acccagaatt tcgattagaa gaatgtcgca
     6301 atgaccaaaa tagctatgga ggcaatcatg atgcctacga ttaacttacc tacaaattta
     6361 taccaagtac caatttcaac acgaccaagt gccaaggctc ctattacaat acctgatgtt
     6421 ggagccacca agttcaagag acctgatgca gcttggaaag ctgtgataac caagcttgct
     6481 ggtacattta caaactcacc aagaggtgcc atgataccca tagaagcccc tgcaagacct
     6541 gatgaagatg gaatcaagaa ggacattggt aggtagaaga gataggtcaa aatgatgaat
     6601 acttgtgaag ataaaccttg caatccttct tcacccatgt ggagaatagt ggcagtaatc
     6661 ataccatcgt tcatgatgac ttgaatacca cgcgcgatag cacaaatcaa agctacgctg
     6721 aaaatatcag ccgcaccgaa catgaaggat tttaccaaac gttcttcact cataccataa
     6781 actaaaccaa ctacaatccc tgcgatacca aagagcattg ctccttctgg gaagtaccaa
     6841 gtaccaagag cagctgttga agaaccaatc acttgaccga tgaacggaag accaatcaac
     6901 caatctttaa attgtgtaaa gatagtaaca cccaaatctt cccatggaat aaagctacaa
     6961 atcatgagaa caaatgttaa tacaaatacc cagagaacac gtttttgagc tggtgataat
     7021 tctgcattca cttcttgagt tgctgttaca ttaaagtgtt gcatgtccgc ttcacgttgt
     7081 ttataaacca aggacttagt cggatcgttt ttcactttct cagcatagtt ggctacaaag
     7141 agaaccccca tcaagagaat aacaacgaag aagaccaagc gccaaatcat accgtccgca
     7201 acacttacac ctgctgcatc agaggctaca accgttgcaa atgggtttac agtagaagcc
     7261 aaacagccga tttgagaacc tagcaagatg atggctacac cagtaatact atcaaaacca
     7321 accgccatca taacaggaac aaggagcggg aagaaagcca ttgtttcttc acccatacca
     7381 taggttgagc ctcctaaggc aaacaaaggc atgaggatgt aaatcaacat tttttcacga
     7441 cctttgaagc gttttacaat agaagcaatc cctacatcaa gtgcacctgt ttcgttaact
     7501 acaccaagaa aggcgccaac catgatgatg aagaatgcta cgttaatcgc tgcttcagtc
     7561 gcaccgtgac caagcatggc acgaactgga gccataaaaa catcgtaaat cccttgtgga
     7621 ttttgagcaa ccttctcata ggtaccagcc accaaacgtc cggcttcatc cacctgatat
     7681 tgaccagctg gaataatcca tgtcatcact gccataaagg caataatgag catcaatatt
     7741 gtataagacg aaggcaattt aaagcctttt ttcacttttt cgctcatcgc aaaaacctcc
     7801 tataatattt taaaagttta tcccctattt aagggaaatt gtatccgtaa taaccgtatc
     7861 aaatacccac ctatcttcaa gagatgtcta tctccttcag tctattatct gaaatcattg
     7921 cttatagata gatacttgat aggaaatgga tgagagggga gggagcttag aagcccagac
     7981 cttcatgttt agattaaagg gaacccttgt gggactatca acactttcac tcccctaact
     8041 ccatcaataa ataacccgtc tagtttaaaa actatatttg catgcagagt acatcaataa
     8101 atagaagcct caacgataca tacagctgct tagcctttga caatgatagt gccgctatcg
     8161 gattcaatca aagcacctaa gttttctaat gaggtaatga ctgcttttga ttgcggctta
     8221 ttgtttacaa aagcaatagc tgcttcaact ttcggtagca tggaacctgg tgcgaattga
     8281 ttttgtttga tatattcttc caattcagcc acagtaacgg tttctaattt ttcttggtct
     8341 ggtttgttgt agttaacaaa gacatagtcc acacctgtca agacgataaa caagtctgca
     8401 tcaaccaatt ctgccaaaca ttgagaagca aagtccttat cgataactgc ttccacaccg
     8461 gtaagagtac cgtctgcttc ttggacaact ggaatacctc cgcctccagc agctacgact
     8521 acctcacctg cattgagaag ggtacgaatg gtaccaattt ctttgattcc tacaggtttt
     8581 ggcgaagcaa cgactttgcg ccagccacga ccggcatctt ctttgaaggt tgcaccagtc
     8641 ttttctgctt ctgctttcgc ttcttcttct gtatagaatg gtccaatcgg cttggtcaaa
     8701 ttttcgaagg caggatcatt cttatccacg ataacctggg tcacaacaga tgctacatct
     8761 ttttcaatgc cttctttgtg caattcattt tccaaggcat tttgcaacca gaaaccgatc
     8821 gaaccttctg tcatggctac caaactgtct agtggaaaag ctgggttctt ttccgaatca
     8881 gcagcaaggt tttgaagaag taagtttcca acctgaggac cattaccatg cgtgataata
     8941 aggtcgtccc cattttttat caattttaca aggtgcttgg ctgtttgtac caaggcctct
     9001 ttttgtgctt tagccgatgg gtcggacgat aaaatggcat tgccacccaa ggctactaca
     9061 attttacgat ttgccatatc tcttgctttt tcctcacttt cgcgaaatag taatcgctta
     9121 cacatctatc ctcaaaatat gggggctggg tgccagcccc cggaggttgc gatgcgttat
     9181 tagatcgtta attattacac acgtggaaca aatggatccc ctaaagtagc cgccatcact
     9241 gctttaatag tgtgcatacg gttttcagct tggtcgaagt gacgagcata tttgctacgg
     9301 aatacttcgt ctgttacttc catttcttct acgccgaatt tttcagcgac atctttacca
     9361 taaacagtgt ttgtatcatg gaaggcaggc aagcagtgca agaagatgag attttcattt
     9421 tctgcttttt tcaccaattc catattcact tggtatggtt tcaagagggc aacgcgttct
     9481 gcaaatttat cttcttcacc cattgatacc caaacgtcag tataaagaac atctgcacct
     9541 ttgactgctt cgtcagcatt atcagtaatg agaacacgtg ctccactttc tttcgcaaaa
     9601 acttcagcca aggcaactac ttcttcttct gggaagagtt cttttggaga gaagatatgg
     9661 acattgacac caaggatagc acctgtcacc aagagagagt tggcaacgtt gttacgacca
     9721 tcaccacagt aaaccagagt caaaccttcc aacttgccaa agttttcttt gacagtcaag
     9781 tagtccgcca acatttgagt tgggtgccat gcatctgtca aaccattcca tactggcaca
     9841 ccagagaatt ctgccaattc ttccaccatt ttttgactga aaccacggaa ttcaataccg
     9901 tcgaacatac gccccaaaac tttagcagta tcttctgtag attctttctt accaagttgg
     9961 atatcatttg caccaagata ttctggatgg gcaccaaggt caatagatgc tactgtgaag
    10021 gcagcacgag tacgtgttga tgttttttca aacaagaggg caatattttt accttccaaa
    10081 taacggtgtg gaatattgcg ttttttcaaa tctttcaaat gagctgagaa atcaatcaac
    10141 cattccaatt ctgcgcgtgt gaaatctttc tctgcaagga aatgtctacc tttaaatacg
    10201 tttgtcattg tctcttctcc taatgtttaa ataaattttg tcaatgcctg gcaccattct
    10261 ccatcttctt gataaacttc agaagttgct tggtagccaa ctgactcata aaaagtcttt
    10321 gcagttaact ccgaatgtat attcaactga tgaaagcctg cttcacgtgc ataagcttcc
    10381 aaagagctga taatcatttt tccatagcct tttccacgaa aattcggtag agtggctatt
    10441 cgggttagac gagcttgacc aggaccgaca ggaagaaaac gtcccgtcga aacaggctct
    10501 tcaccaataa agagattggc gtaaactctt ccttccgtat cattctggtc aaattcacct
    10561 tcctttgcaa taccacgctc aaggacaaat accttatagc ggacatagat agaggctgcg
    10621 cgttgccatt cttccgaacc gattgttgtt cgttctatac ttggcattag atgtcttccc
    10681 gttcaaacgg cattgacata caacgaggtc caccacgacc gcgaaccaat tcgcttccgc
    10741 caatcttaat caaacgaagc cctttggact cgaggatagc attggtaatc gtattgcgtt
    10801 tgtaaacaac taccacacct ggtgcaatag tcaatgtatt tgaaccatca ttccattgct
    10861 cacgaccagc agcaaccata tcaccgccac cacaacggat caattctact ttttcaagac
    10921 caaggttcgc agccaaaagt tctgccaaat caccatgttc ttcttcaata tgaagcgtgt
    10981 cattttcata ggttacagag aagacacgaa ggtcaccttc gatttctggg tggatagtaa
    11041 acttatcata gtcaaccatg gtaaataccg tatccaaatg catgaattta cggttgttag
    11101 caaattcgaa tgccaatact ttcttgaagc caacatgctg ttcaaagata tttaccaata
    11161 gtttttcaat tgatgctgcg tcagtacgtt gtgaaatacc tactgccaaa acatcttttg
    11221 aaagaaccaa ttcatcccca ccctcgatac gagtagtttc ttcacgattg taaaccaatg
    11281 gaacttttcc accatattct gggtgatgag tgaagatgta tttaccatac aaggtttcac
    11341 ggttccgtgt ttctgaatac atgtggttaa gagaaacagc attaccaatc gtagcaaatg
    11401 gatcccgtgt gaagtaaagg tttggcattg ggtctattgc aaatgggtaa gaggattcaa
    11461 ccaaatctgt caaacctttt tcttcagcag ggatttctgg caattctgcc ttttgaatac
    11521 cggccatcgt tttctcaacc agttcttgat tatctttaat agacaacaac aacttgcgaa
    11581 ttgccttctt agtcgcccga ccacgaatgt tagcttcttc gagatattca tcaataaatt
    11641 gttcacgaat ttcaggtgta acaagtgact cagcagctaa tttttccaag tagagcactt
    11701 ctacaccttc atcacgaaga gcttgagcaa aggcatcatg ctctttctgt gcatcttcta
    11761 agaatggaat atcatcgaag agtaggcgtt ctaggtagtc aggcataagg ttttcaattt
    11821 ccttacctgg tctgtgtaac ataacttttt tcagcttacc aatttctgaa aaaacatgaa
    11881 ttggatggtt tgacatgatc tgtcctcctt gtagatttat acatgatggg aaacgaggag
    11941 gaccgtacgc tttcctgcct ctcatttcct ctttacatcc atagtttaac agagtaaaaa
    12001 aacaaaaact tgaaaacgct cacatttatt ttgagaaatt cttcacattt ttaaccttat
    12061 tta
//
LOCUS       DBNFEG010000049        11926 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.049, whole genome shotgun sequence.
ACCESSION   DBNFEG010000049 DBNFEG010000000
VERSION     DBNFEG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11926
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.049"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            47..1393
                     /gene="gdhA"
                     /locus_tag="AC1PNG_000929"
     CDS             47..1393
                     /gene="gdhA"
                     /locus_tag="AC1PNG_000929"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HIP6701440.1"
                     /translation="MSNAKAYIQASFEAVKARNPHETEFLQAVEELFSTLEPVFEAHP
                     EYIEENILARIVEPERIISFRVPWTDKYGNVQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQSILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEIMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYMYGQYKRLRQFDAGVLTGKSLGFGGSLIRPEATGYGLVY
                     FTDNMLAANGKSFKDQTVLISGSGNVAQYAVQKATELGAKVISVSDSNGYIIDETGID
                     FDLLVDIKEKRRARLTEYATEKATAKYFEGSVWNYDGKADIALPCATQNEINGEQAAA
                     LVKNGVYCVAEGANMPSDLDAIKVYKENGVLYGLAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMANIFNTAKETAEKYDLGTDYLAGANIAAFEQIADSMIAQGLV"
     gene            complement(1628..2215)
                     /locus_tag="AC1PNG_000930"
     CDS             complement(1628..2215)
                     /locus_tag="AC1PNG_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HIP6701441.1"
                     /translation="MQTKDFVVENGQMYYKKKPLHKQPLFWTTIAGAVLSFILGVTCF
                     IFMIGLSSTNFDNWDTGSDGYIDETVEYTEYQVGDSVDFSDGLHVTVTSMGKDDSIEL
                     VDSYYSTAYVVEMEVENSTAKELYFDEYYFSLMDPVSQIPFTLDLRTYDVNLVEKLKP
                     GEKVQVKLIYGIDNETNFGFTYEDAMWTELVAEGI"
     gene            complement(2226..3074)
                     /locus_tag="AC1PNG_000931"
     CDS             complement(2226..3074)
                     /locus_tag="AC1PNG_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIP6701442.1"
                     /translation="MNDFITKYLSQFNIEAIVTAGLNKVLSLVLLFVAFYIIKKIAKA
                     SVKKVLVPSLKVSTQDIGRQKTISRLVESILNYLLYFILIYCILSILGLPVSSLLAGA
                     GIAGVAVGLGAQGFLSDLVNGFFILIERQFDVGDVVKLTNGPITISGTIVSMGIRTTQ
                     VRDADGTLHFIPNRNILVVSNQSRGDMRAQIDIPLKFNTDLEQVYRVIEEVNLREISN
                     FDQITGVTVLGPQNAISGQFVFRVNLFVANGQQNKIYHQFFGFYQDALRQAGIDLPSS
                     GPTVLK"
     gene            complement(3139..4473)
                     /locus_tag="AC1PNG_000932"
     CDS             complement(3139..4473)
                     /locus_tag="AC1PNG_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIP6701443.1"
                     /translation="MLELFKAINNLVWGPPLLLLLVGTGVYLTLRLGVFQIGKLPTAF
                     RLIFSSDQSGQGDVSSFAALCTALAATVGTGNIVGVATAITTGGPGALFWMWVAAFFG
                     MATKYAEGFLAIKYRTKDANGQAAGGPMHYITLGMGQKWKPLAVFFAISGVLVALLGI
                     GTFSQVNSITASLETSFGLEPPLVSVITAISIAFVVFGGIEKISDVSTKIVPFMAILY
                     ILASITVLAVHWDQLLPTLALVFKSAFTPAAAVGGFAGATVQQAIQRGIARGVFSNES
                     GLGSAPIAAAAAKSDNPVEQGLISMTGTFIDTLIICTLTGFTILVTDQWSLEGLAGAP
                     LTQAAFATVFGNTGSIALTISLVLFAFTTILGWSYYGERCIEFLFGTKAILPYRLVFV
                     AMVALGGFLKLDLIWTIADIVNGLMALPNLIALLALSPVIIKETRQYFAKKK"
     regulatory      complement(4579..4683)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(4896..5528)
                     /locus_tag="AC1PNG_000933"
     CDS             complement(4896..5528)
                     /locus_tag="AC1PNG_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6701444.1"
                     /translation="MFSIQSIQKKFGKRSLFSDVTLDLQAGKVYALIGASGSGKTTLL
                     NILAKLESYDAGDILYKGKDLKKLKPHLFFREELGYLFQNFGLLESQTIKENLDLGLV
                     GQKLSKDERKSRQEAALDAVGLSYLDLSQPIYELSGGEAQRVALAKVILKNPPLILAD
                     EPTAALDPKSSQEIMDILLSLKSPERLIVIATHNPLIWERADLIIKMEDL"
     gene            complement(5530..7512)
                     /locus_tag="AC1PNG_000934"
     CDS             complement(5530..7512)
                     /locus_tag="AC1PNG_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HIP6701445.1"
                     /translation="MKGKFVLVSTIILAVFMIWLGVSQKETMLVHYYPSVTSLSVSED
                     VTYSDVRQRLEEYSQQTDSVIARRVIEPSTSGGRTFSYDNFSQSSLPRGLEEFQASEK
                     VESALLTKYFIFQGKATVEELRSLLVSLGFDEVQIRKPSTIATLLAFLTQGGQFLAVL
                     VFLITYMALVVVANVRQLRTAGIRLIAGDSRWHLFLLSLQENAKEIALTIPFAVLPAV
                     GLAYLIGLDSYSVYYLVAALVGYHFLLGLIALFFAATFTLAIRIYHFLPLLKGKMPLQ
                     GILTIMVMGQMLALLVVSLGMAQTVYYSGIWQEYQAGARQWEKEEDYYSLAWNIAADG
                     HSGLNSPENWYPLLKQALEKDGALFVKSNLNAYLMGSQLEDGTSLDSYHPAGNTLYVS
                     PNYLQIQDVDLSDEALPSLQEGEFQLLLPEKLRPESDIYLHLYQDYINRMVRPANQAS
                     SATIKGKLAYLKDGQKQFIYNHRSSQHVQYLTDPILVVLAPSSLGKESADFWLNEVDS
                     SILFKNRDKLLRELKARNLFQFVNEVRSSSSLFTELLDRIRIETISTSMGAILGIVTS
                     IILFNTMNLLYFEEFKRDIFIKEIAGMDFLGIHKKYLTFQLLSLLLALGASMVVTGHM
                     FISSISFALFMVNALCLLRWQARKDGTWNIRILKGA"
     gene            complement(7532..9517)
                     /locus_tag="AC1PNG_000935"
     CDS             complement(7532..9517)
                     /locus_tag="AC1PNG_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HIP6701446.1"
                     /translation="MKKVFTYLSTFSVCLFCIWLVSLQLDSLIYHYYPSVSILSRGEE
                     VTYEEVSDALTNLAEKENSLIAKTITETDDSGRTTTSYDVFGKGELPQGLVKADRLVA
                     SQANLAENYYVVKGNLDIKVVENVLQSLGLVDSYIYRPTLLNSLSLFLGRGAPSLGLI
                     IFILTNLALTVVGQTVYLRSAGIEMLSGVRSWQIASRFLRVDGRDIALSTGGALVFGL
                     IAILILGWPMVYYRFVAIGIAFYACLLFFLSLSTSIGFMIALNISQLQKLIKGALPLK
                     AIFAILLVGQCLAVCIISIGASRTLTYVGVLHTLTIGEKAWQERQDVFSLSLNREAFI
                     ADEAMVQQQRMAWSHVILESYAKNNVMLVQHHLRHNTIKEHTIIESSKEIPLERVLYV
                     TPNYIQSEGGLCDFNQLEEIKQLGKGEIALLLPKSLQNDASVYQAYFEDMVGKLLADG
                     EKSISLYSNVYYISDEKRWFIYNHTPINYEQFLQAPLIVVLSPESFEETSYFWENALP
                     DFVFFKDKEMLEQLLEKYNLRNTIGSLLSSRQQYNTLRKNVQLEILMTLSPTILGIFT
                     SILLFNTMNLLYFETFKREIAIKRIAGMRFIELHGSYLGEQVGSALVGMGLAMFMTKS
                     VIVSLGVVVALLINSWMLLNKQAKKAEKIQLSVLNGR"
     gene            complement(9892..10185)
                     /locus_tag="AC1PNG_000936"
     CDS             complement(9892..10185)
                     /locus_tag="AC1PNG_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701447.1"
                     /translation="MDLNDYYTDIDPDFSFRELRRKIERYRIVMQARVLLDKRDTIKR
                     TGKTDIQKIASETKQTIQTMRGRLETSIKGSVRTGIEEAVDKHSSLYDTITQS"
     gene            complement(10172..10561)
                     /locus_tag="AC1PNG_000937"
     CDS             complement(10172..10561)
                     /locus_tag="AC1PNG_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701448.1"
                     /translation="MESDVIWERIRKREQELFDLEDDYNQEKNKIEARQEDLEQRQNA
                     LKLLIEREQEEMRCFLSRHSLDYDAALSFFQELDQLQEESFYQYRQEMDQLFQQEERL
                     SQQYRTDLYRLEDTISQLRRDYSNGLE"
     gene            complement(10546..11760)
                     /locus_tag="AC1PNG_000938"
     CDS             complement(10546..11760)
                     /locus_tag="AC1PNG_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIP6701449.1"
                     /translation="MISDKDYRDISKVVYDVDLLKAPKTPLIQDTPVVNGSFIIIEPP
                     VDAGNGMQAMAVAPIKGYDTDNKPIPDTSQVVIAYAGTNDGDIKDWATDISMIGGGNT
                     ATFTVPGQNVPYISQSITAVAFADQIVQRYPDATITITGHSLGGSLAMYVALKKDLPA
                     ITFNGPDIHNMISEDEIEYMQQHRAQFRNYRNKYDFLGDITGNKTKSAIYPAVKNIPG
                     DAIADHMLNAWKFNKYGQLIDANGQVVSTFTGAPLEDTRLAMKRWKKQKTALSKGGLS
                     RSEEIFLDSEQARTISAGIASVADAGQMSIFAHCDDYVHQAEELWNSIDFHSYSALSY
                     WEVCALFSEYGVSQETIVREIEETMDIYRQRAEIATEKFTILDTQIIQAINTMLETDK
                     RLAGDFEKWNQM"
BASE COUNT         3624 a         2714 c         2210 g         3378 t
ORIGIN      
        1 aaattgtcag ataatttaat aaataaaaaa gaaatcaggt gatcccatgt caaatgccaa
       61 agcttacatc caagcttctt ttgaagcagt caaagcccgc aacccacatg aaacagaatt
      121 cctccaagct gtagaagagc tcttctctac acttgagcct gtttttgaag cacacccaga
      181 atacatcgaa gaaaacatcc tggctcgtat cgttgagcct gagcgtatca tcagcttccg
      241 tgttccatgg acagataaat atggaaatgt ccaagtcaac cgtggctacc gtgttcagtt
      301 caactcagct gtaggtcctt acaaaggcgg tcttcgcttc cacccaactg taaaccaatc
      361 catcttgaag ttcctcggtt ttgaacaaat cttcaaaaac gtcttgactg gtcttccaat
      421 cggcggtggt aaaggtggtt cagactttga tcctaaaggc aaaacagacg ctgagatcat
      481 gcgcttctgc caaagcttta tgactgaatt gcaaaaacac atcggacctt cacttgacgt
      541 ccctgctggt gacatcggtg tcggtggtcg cgagattggt tacatgtacg gtcaatacaa
      601 acgtctccgc cagtttgatg caggtgtctt gactggtaaa tctcttggct ttggtggttc
      661 attgatccgc ccagaagcaa ctggttacgg tttggtttac ttcactgata acatgttggc
      721 agcaaacggt aaatccttca aagaccaaac agtccttatc tcaggttctg gtaacgttgc
      781 acaatatgct gttcaaaaag cgactgaact tggtgcaaag gttatttctg tttcagactc
      841 aaatggttac atcattgacg aaactggtat tgacttcgac ctcttggtgg acatcaaaga
      901 aaaacgccgc gctcgtttga cagaatacgc tacagaaaaa gcaactgcta agtacttcga
      961 aggttctgta tggaactacg acggaaaggc tgatattgcc cttccatgtg cgactcaaaa
     1021 cgaaatcaac ggtgagcaag ctgctgccct tgtgaaaaat ggcgtttact gtgtggctga
     1081 aggtgccaac atgccatctg acctggatgc tatcaaagtt tacaaagaaa atggcgttct
     1141 ttacggtctt gcaaaagctg ccaacgctgg tggtgtagcc gtatctgccc ttgaaatgag
     1201 ccaaaacagc cttcgcttgt catggactcg tgaagaagta gacggccgtc ttaaagacat
     1261 catggcaaat atcttcaaca cagccaaaga aactgctgaa aaatacgacc ttggtacaga
     1321 ctaccttgcc ggtgctaaca tcgcagcctt tgaacaaatt gcggatagca tgattgccca
     1381 aggtttggta taagcttact tttagtttac taaactataa taaaaacgcc cccaaaattg
     1441 acgtgctaat tttgggggta tttttcatta aaagaagagg agaacagtgg ataaacatgt
     1501 tctccttttt aaaatgtaaa aataacagat aagtcggata tagtacaatt accaagccga
     1561 acaatctgaa ccaaggggcg tataaacccg caaaattgtg caatatttaa aactttttct
     1621 acagcaatta gataccttct gcgactaatt ccgtccacat ggcatcttcg taggtaaaac
     1681 caaagtttgt ttcattatct ataccgtaga taagttttac ttgtactttc tctcctggct
     1741 tcagcttttc aactagattc acatcatagg tccgcaaatc cagggtaaag ggaatctgac
     1801 taacgggatc catgagactg aaatagtatt catcaaaata gagctctttt gcggtcgaat
     1861 tttcaacttc catttcaaca acataagctg tgctgtagta gctatccact agttctatgc
     1921 tatcgtcttt gcccatagat gttactgtta catgaagacc gtcagaaaaa tcaactgagt
     1981 caccaacttg atactccgtg tattccacag tctcatcaat gtatccgtca ctacctgtat
     2041 cccaattatc aaaatttgtt gagcttaggc cgatcataaa aataaaacag gttactccta
     2101 agatgaagga gaggactgca ccagcaatgg tcgtccaaaa gagaggctgt ttgtgcaagg
     2161 gtttcttctt ataatacatc tgcccatttt caacgacaaa atcttttgtt tgcataataa
     2221 actccttatt ttaaaacagt tggcccactt gaaggtaaat ctattcctgc ttgacgcaga
     2281 gcatcttgat aaaaaccaaa gaattgatga tagatcttgt tctgttgccc gttggctacg
     2341 aaaagattaa cacgaaatac aaactgtccc gaaatagcgt tttggggacc taatacagtc
     2401 acacctgtaa tctggtcaaa attgctgatt tctctcaaat tgacctcttc tattacgcga
     2461 tagacttgct ccaaatcggt attaaatttg agcggaatat cgatttgagc acgcatgtcc
     2521 cctcttgact gattggacac cactagaatg ttgcgattgg gaataaaatg caatgtccca
     2581 tctgcatcac gtacttgtgt tgttcggatt cccatgctaa cgatggttcc tgaaatggta
     2641 atgggcccgt tagttaactt aaccacatct ccgacgtcaa actgtcgttc aattaagata
     2701 aagaatccat tgaccaagtc tgagagaaaa ccttgtgcgc ccagcccaac agctacccca
     2761 gcaatcccgg ctcctgcaag aagactggat accggtaaac cgaggatact caaaatacag
     2821 tagataagga taaagtagag taggtaattt aagatgcttt ccaccagacg actgatggtc
     2881 ttctgacgac caatgtcttg tgtagatacc tttagagagg gaaccaaaac ctttttcaca
     2941 gaagctttgg ctatcttctt gataatgtaa aatgcaacaa agagtaaaac aagcgataaa
     3001 accttattta gacctgctgt cacaatggcc tcaatattaa attgagacaa atacttggta
     3061 ataaaatcat tcatgagata attatagcag aaaacaccct aaaagaacaa aaagacagcc
     3121 aagtggctga cttttatctt atttcttctt tgcaaaatac tggcgtgtct ccttgatgat
     3181 aacaggagag agagctagta aggcgatcaa gtttggtagg gccatgagac catttacaat
     3241 atcggcaatg gtccaaatca agtctagttt gaggaaacca cccaaggcaa ccatagctac
     3301 aaagaccaga cggtaaggta agatggcttt ggttccaaat agaaactcaa tacagcgttc
     3361 accatagtaa gaccatccga gaatagttgt aaaagcaaaa agtaccaaac taatcgtcaa
     3421 ggcaatagat cctgtattgc caaaaacagt cgcaaaggct gcctgcgtca gaggagcacc
     3481 tgccaagccc tctagcgacc actggtcagt tactagaatc gtaaaacccg ttaaggtaca
     3541 gatgataagt gtatcaataa aggtacccgt catagaaatc aagccctgtt caactggatt
     3601 atcagacttg gcagccgcag cagcaatagg cgccgaaccc aaaccagatt catttgagaa
     3661 aaccccacgc gcaatcccac gctgaattgc ttgttgcaca gtcgcacctg caaaaccgcc
     3721 cacagccgct gctggagtaa aggcagattt gaaaactaag gccagggttg gcaagagttg
     3781 atcccaatgc acggccaaga cagtaatact agctaagata tacaaaatcg ccatgaaagg
     3841 aacaattttg gtcgaaacgt cagagatttt ttcaatccca ccaaaaacaa cgaaggcaat
     3901 tgaaatagcc gttattacac tgacaagtgg cggttccaat ccaaaactcg tttccaaaga
     3961 agcagtgatg gaattaactt gcgaaaaagt tccaatcccc aagagagcaa ccaaaacacc
     4021 tgaaatagca aagaaaactg ccaagggttt ccacttctgt cccattccca aggtgatata
     4081 atgcataggt ccaccagctg cttgcccatt ggcatccttg gttctatatt tgatggccaa
     4141 aaagccttcc gcatacttgg ttgccatccc gaaaaaggcc gcaacccaca tccagaaaag
     4201 agccccagga ccacctgtgg taatagctgt cgcaactccg acaatatttc ctgtaccaac
     4261 tgtcgctgcc aaagctgtac aaagggccgc aaagctggac acatctccct gaccagactg
     4321 gtcactggag aaaatcagac gaaaagccgt cggcaatttg ccaatctgaa atactcccaa
     4381 acgaagagtg agataaacac ccgttccgac caatagtaac aagaggggcg gcccccaaac
     4441 aagattgttg atagccttaa atagttctaa catagttcct cctaagcgtt tctaatccga
     4501 taaagctgcc ggaaaaacaa aaagaggctg aacacttcaa cctctagaat caacgtgaaa
     4561 atatagaaaa agcctatttt tcaacgctct gtccttttac ctgagagttt cagtagctct
     4621 acttttccaa gcaggactac tttgcccctt cggtgccaaa atggtctctc cagagttccg
     4681 tccattgaac agtcagtttc ccttctaccc aatatcaacc ggttattcag ttttatctag
     4741 ttttatttta atctaatttc acgaaaaaat caagaatatt ttctgatact caatgaaaat
     4801 caaaagtagc ctaggaaacg aagccgaaga tagaactgga gttcatcaag gtaagttgac
     4861 aacggataat tttgattttc gaagagtatg aatttttaca agtcttccat ttttatgata
     4921 aggtctgctc gctcccaaat caaaggatta tgggtcgcaa tcacaattaa ccgttctggg
     4981 ctcttcaagg ataacaaaat atccataatt tcttgggagc tcttaggatc caaggcagcc
     5041 gtcggctcat ctgctaaaat caatggcgga tttttcagga tgactttagc aagagctacc
     5101 cgctgggctt cccctcctga gagttcatag ataggctggg acaggtccag ataagacaga
     5161 ccaactgcat caagtgccgc ttcctgcctc gatttccttt cgtctttgga caatttctga
     5221 ccaaccagtc ccaaatccag attttctttg attgtctgac tttctaaaag accaaaattc
     5281 tgaaagagat agcctaattc ctccctaaaa aagagatggg gcttcaactt tttcaaatcc
     5341 ttccccttgt agagaatgtc acctgcatca taggactcca acttggctaa aatattcagc
     5401 aaggtcgttt ttccgctacc actggcacct atcaaggcat agacctttcc agcctgcaag
     5461 tctagcgtta catcagaaaa caaactgcgt ttcccaaatt tcttttgaat tgattggata
     5521 gaaaacatac taggctcctt tcaaaatacg gatattccaa gttccgtctt tcctcgcctg
     5581 ccacctcaat aaacagaggg cattgaccat aaacaaggca aaactgatac tacttatgaa
     5641 catgtgacct gtcactacca tgcttgctcc aagagccaac aagagggaaa gcagttggaa
     5701 cgttaggtat ttcttatgga ttcccaagaa atccatccct gcaatttctt tgataaaaat
     5761 atcacgttta aactcttcga agtataatag attcatggta ttaaacagga taattgatgt
     5821 cacaatccct aaaatagcac ccatagatgt tgaaatggtc tctatccgaa tacggtctaa
     5881 aagttctgta aagagggagg aactacttct cacttcattg acaaactgaa acagatttct
     5941 tgccttcaat tcgcgcaaaa gtttatccct gtttttaaat aaaatactgc tatctacttc
     6001 attcagccaa aagtcagcag attcttttcc cagacttgac ggtgccagca caactaaaat
     6061 agggtctgtc aggtattgca catgttgact agaacgatga ttgtagataa attgcttctg
     6121 cccatctttt aaataggcaa gttttccctt gatggtagct gagcttgctt gattggcagg
     6181 tctaaccata cgattgatat agtcttggta aagatggaga taaatatcgc tttcagggcg
     6241 caatttttca ggtaagagaa gttgaaattc tccttcttgt agagaaggta atgcttcgtc
     6301 cgacaagtct acatcctgta tctgcaaata atttggacta acgtagaggg tgttgcctgc
     6361 tggatggtag ctatcaaggc ttgtcccatc ttccagttgg gagcccatta aataggcatt
     6421 caaattactt ttgacaaaga gcgcgccgtc tttctccaaa gcttgcttta gtaaagggta
     6481 ccaattttca ggcgaattta gtcctgagtg accatccgca gcaatgttcc aagccaaact
     6541 atagtagtcc tcttctttct cccactgccg ggcacccgct tggtattctt gccaaatccc
     6601 tgaataatag acggtttgag ccatgcccaa tgacaccacc agtagagcca gcatttgccc
     6661 cataaccata atggtcagga ttccttgcag gggcattttc ccttttaaca agggtaaaaa
     6721 gtgatagatt cgaatggcca aggtaaatgt agcagcgaaa aaaagggcaa tcaaaccaag
     6781 caagaaatga tagcctacta gagctgcgac cagatagtag acagaatagc tatctaatcc
     6841 aatgaggtag gccagtccaa ctgctggaag aaccgcaaat gggatagtta gagctatctc
     6901 tttcgcattt tcctgtagag atagtagaaa taaatgccaa cgtgaatctc ctgcgatcaa
     6961 acggatacct gctgtgcgca attgccttac attggcaacc actaccaaag ccatataggt
     7021 aataagaaag accagtacgg ctaagaattg accaccttgt gtcagaaatg cgagtaaagt
     7081 agcaatagtc gaaggcttcc gaatctgaac ctcgtcaaat ccaagcgaaa caagaagaga
     7141 ccgaagctcc tctactgtcg ctttcccttg gaaaataaaa tatttggtta aaagcgcaga
     7201 ctccaccttt tctgatgcct gaaattcctc cagtcctctt ggtaaggaag actgactaaa
     7261 attatcataa gaaaaggtac gtccacctga tgtacttggc tcaatcaccc gcctggcaat
     7321 cacactatcc gtctgttgag aatattcctc taatcgttgt cttacatctg agtaagtcac
     7381 gtcctcagat accgacaagc tggtcacaga aggataataa tgaactagca tagtctcctt
     7441 ttggctgacg cccaaccaaa tcataaagac ggctagaatg atcgtcgata caagtacaaa
     7501 ttttcctttc attctaactt cacctcatta actatctgcc atttaataca ctcaactgga
     7561 ttttttctgc ctttttcgct tgtttgttca gtaacatcca actatttatc aacagagcca
     7621 caactactcc taggctaaca ataactgact ttgtcataaa cattgccaaa cccatcccaa
     7681 caagagcgga gccgacttgt tctcctagat aagaaccatg caattcgata aaacgcatcc
     7741 ctgctatccg cttgatggct atttctcttt tgaaggtctc aaagtataga agattcatcg
     7801 tattgaacaa tagtatggaa gtgaatattc caaggattgt cggagatagg gtcataagaa
     7861 tttcaagttg aacatttttt ctcagggtgt tgtactgttg cctgctagat aacaagctac
     7921 ctattgtgtt tcgtaaattg tatttctcca acagctgttc cagcatctct ttatctttga
     7981 aaaatacaaa atctggtaag gcattttccc aaaaatagga cgtttcttca aaactttcag
     8041 gtgatagaac aacaatcaac ggagcctgta aaaattgctc ataatttatc ggagtatgat
     8101 tgtaaatgaa ccatcgcttc tcgtctgaaa tgtaataaac gtttgaatac aaacttatgg
     8161 atttttctcc atccgcaagc aatttcccta ccatgtcctc aaaatacgct tggtaaacac
     8221 tcgcatcatt ttgcaaactc tttggtaata ataatgcaat ttctccttta cctaattgct
     8281 ttatctcttc cagctgattg aaatcgcaca gtccaccttc tgattgtata taattcggcg
     8341 tcacataaag taccctttct agaggaattt ccttgctgga ttcaatgatg gtatgttctt
     8401 ttattgtgtt atgccgtaaa tggtgctgga ctaacattac attatttttc gcatatgatt
     8461 ctaaaattac atgagaccaa gccattcttt gttgctggac catcgcttca tcagctatga
     8521 atgcttcacg attcaaagac agactaaaaa catcttgtct ttcttgccaa gccttttctc
     8581 ctatcgttag tgtatggaga actccaacgt atgtcaacgt tcgactagca ccaatagaga
     8641 tgatgcaaac cgctaaacac tgacctacta gtaaaatagc aaaaatcgcc ttgagaggca
     8701 aagctccctt aattagcttt tgtaactgag aaatgtttaa tgcaatcatg aatccaatac
     8761 ttgtcgataa agataggaaa aaaagcaaac aagcataaaa ggcgataccg attgctacaa
     8821 aacgataata taccattggc caacccaaaa ttaaaatagc aatcaatcca aaaacaagcg
     8881 ctcctcctgt agacaatgct atatctcttc catccactct taaaaaacga ctagcaatct
     8941 gccaactcct cacccctgaa agcatctcta tacctgccga ccgcaagtag acagtctgcc
     9001 caactactgt caatgcaaga ttggtgagta taaagataat aaggcctaag ctcggagctc
     9061 ccctaccaag aaatagactt aaactgttta atagggtcgg tctataaata tagctatcca
     9121 ccaatcccag tgactgtaag acgttttcta ccactttgat gtccagattc ccttttacaa
     9181 catagtaatt ttcagctaag ttggcctgac tagctactaa cctatccgct ttgaccaagc
     9241 cctgtggaag ctcccccttc ccaaaaacat cataactggt agtagttcgc ccactatcat
     9301 ctgtttctgt tattgtctta gcaattagac tattttcctt ttcagctaaa tttgtcaggg
     9361 catccgacac ttcttcatag gtcacttctt cccctcgact taatatggaa accgatggat
     9421 agtaatgata aattaaagaa tcgagttgta gactcactaa ccatatacag aagagacaga
     9481 cgctaaaagt agataaataa gtaaatactt ttttcataac gaacctcatt tgcacaaaaa
     9541 ggaggtggac tccaccacct ccctatcatt agaacccgta accaaaatca acatgttttc
     9601 caaagtttat tgtatcactt cgctattatc tctatcttaa catataaaca aaacaaaaac
     9661 aaggttgctt gcattcttac catgccgtac gcatgaagaa aatgcgtata accatttcct
     9721 agacatctgc attttcaaag cgttttcatg aaaccaatac ttcaaccagg atcgtctttc
     9781 atttccaaag ctataaacag ttttctacca aaaacggatg ccaacagcaa gaaaatagtt
     9841 ttgaaaacga aaatttcttt attcgcttgt tatgcgtaac tatatctcag cttaactttg
     9901 ggtgattgta tcatacaagc tgctgtgttt atccacggct tcttcaatcc ctgtacgtac
     9961 actccctttg atactggttt ccaaccgtcc tctcatcgtt tgaatcgttt gcttcgtttc
    10021 gctggctatc ttctgaatgt ctgtcttccc tgttcttttt atagtgtcgc gtttatccaa
    10081 caagactcta gcctgcatta ctatgcgata gcgctctatt ttccgccgta gttctcggaa
    10141 agagaaatca ggatctatat cggtatagta atcattcaag tccatttgaa tagtccctcc
    10201 ttaattggct aatagtgtct tctaagcggt agaggtctgt ccgatattgc tgggacagcc
    10261 tctcctcctg ttgaaacagt tgatccatct cttgacggta ctgataaaag gattcttctt
    10321 gtaattgatc caactcttga aaaaaggaca aggccgcatc ataatccaag gaatgacgac
    10381 tgagaaagca acgcatctct tcctgttctc tttctatcaa gagttttaaa gcattctgac
    10441 gctgttctag gtcttcttga cgtgcttcaa tcttattttt ctcttggtta tagtcatcct
    10501 ccagatcaaa taattcttgc tctcgcttac ggattctttc ccatattaca tctgattcca
    10561 tttctcaaaa tctcctgcta gtctcttatc tgtttccagc attgtgttga ttgcttgaat
    10621 aatttgagtg tccaagatgg tgaacttttc tgtagctatc tctgccctct gccgatatat
    10681 atccatagtc tcttcaatct ctctgacaat ggtttcctga ctaacaccat actctgagaa
    10741 aagggcacaa acttcccaat agctgagagc tgaataggaa tgaaaatcaa tcgaattcca
    10801 caactcctct gcttggtgta catagtcatc gcaatgggca aaaatactca tttgccctgc
    10861 atctgcaaca ctagcaatcc ctgctgagat ggttctggcc tgctcactgt ccaaaaaaat
    10921 ctcctcacta cgacttaggc cacctttaga taaggctgtt ttctgctttt tccagcgttt
    10981 catagccaaa cgagtatctt ccaaaggagc accagtaaag gttgatacca cctgaccgtt
    11041 ggcatcaatc agctgaccat atttgttaaa cttccaagca tttagcatgt gatcagctat
    11101 agcatcccct gggatattct tcaccgctgg ataaatagct gatttcgtct tatttcccgt
    11161 aatatctcca aggaagtcat acttatttcg atagttacga aactgtgctc tatgctgctg
    11221 catatactct atttcatctt ctgaaatcat attgtgaatg tcgggaccgt taaaggtaat
    11281 agcaggaaga tctttcttca aagcaacata catcgccaga gagcctccta acgaatgccc
    11341 tgtgattgtg atggttgcat cgggatagcg ttgtactatc tgatctgcaa aagctaccgc
    11401 agttatagac tgagatatat aggggacatt ctgaccggga acggtaaaag tagccgtatt
    11461 ccctccgcct atcatagaaa tatctgttgc ccaatcttta atgtcgccat cattagtccc
    11521 tgcgtaggca ataacgactt gggaagtatc tggaatcggt ttgttgtcag tgtcgtaacc
    11581 cttgatcgga gcaaccgcca tggcctgcat accgttgccc gcatcaacag gtggctcgat
    11641 gatgataaaa ctaccattta caactggagt atcttgtatt agtggagttt taggtgcctt
    11701 taataggtct acatcataaa ccactttaga aatatctcta taatctttat ccgatatcat
    11761 ggcaactcct tatagtattt tacttgtata ttactaattt ctgttactgt ggttgctact
    11821 ttaggaacta agtaatctcc tgtttgagat tttccaccat ttattgtaat atcatcttct
    11881 ccccaagata caaaagtaac atggaatttg tcatttataa ttgcat
//
LOCUS       DBNFEG010000050        11716 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.050, whole genome shotgun sequence.
ACCESSION   DBNFEG010000050 DBNFEG010000000
VERSION     DBNFEG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11716
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.050"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1669
                     /locus_tag="AC1PNG_000939"
     CDS             <1..1669
                     /locus_tag="AC1PNG_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HIP6701450.1"
                     /translation="ANSSQIIAGVGQLQSGASQVEQLVTGANQLQAGLEQLASSTSLS
                     AEQSSQIQALLTGLPQLQAAINQLNDSLSSIGGLAVDTSTLSSLLTEMGAQAQGLLTA
                     AQADKTAGIEALQATATYQHLPADQQAELVGALQNSPSATAAVAQTILIQLSQLSQTL
                     SSLQSLSGMETQMSQLQSAVGQINTAANQALPGATTAIENLSSGLSQVNTALNQQVLP
                     GTQVLTGGVSQLQTQLSSGASQLMSGVTAYTAGVAQLAAGGAQLVANNSSIQSGGGQL
                     TSGLATLASNSSQLVSGSGQLALGSQQLITGADQLASGGQTLTSGITSLRTGSETLTN
                     SLSSASQQLSVVSVEDKNAQAVSQPVTLEHSDQDDVKTNGVGMAPYMVSVALMVAALS
                     ANVIFVKHIDNRSYKNRWDWAKGKLLLNGIIASLAAVILYGVLRLIGIEPAHPMATLG
                     LILLASWTFMALVTALVGWNNRFGSFASLIILLLQLGSSAGTYPIELSPRFFQVIQLY
                     LPMTYSVSGLRQTVSMVGNSSHQVWMLSLFLVGFMGLGLLIYNQKDE"
     gene            complement(1721..2779)
                     /locus_tag="AC1PNG_000940"
     CDS             complement(1721..2779)
                     /locus_tag="AC1PNG_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIP6701451.1"
                     /translation="MKKKLTIAIIIGFVSLIAGLILAGIGFFTGGITRLEEVAAPTEV
                     HKTFTDLNTIKIDFIPHSVYIKESSDNNYHVTYANSDNNIQKPLKLSEKDGVLTLSAQ
                     ELEFAIEGIMQYLGESLAQRRINVFSVTIEVPKGKTLDKLEGHNLHFYDFGGFTIENI
                     HIKEANLSGGVTLDKVTIDSGQIEAGYFQATQSTLKDMHINLQGSSVRLYETSLENVT
                     IEGYSQLDSRQTTLLGDNRFTPSDTYSSTTFLDVTDKSLADSNLLITNQIDKKKLVEA
                     QGYYYEDGNDLGEMVNQDPYLKERLGEVGIFTSDKYTKFPVKTENDLQTLTLENKGSK
                     NKLTIEATNATINLGTPK"
     gene            complement(2776..3531)
                     /locus_tag="AC1PNG_000941"
     CDS             complement(2776..3531)
                     /locus_tag="AC1PNG_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HIP6701452.1"
                     /translation="MTRTEYMEQLEKYLKKLPHKEYFEAISFFNEYFDEAGPERETEI
                     IEELGSPKEAASELINNMLNKQIQEEKEQQEPIQLNWKLWVGLGALSMTGLFSFFLLF
                     IMGEFIGVIPLFATLILGAFFLGRYFRNFSQTKRTLWLAILAVISLPIAIPLLLILLA
                     SLLGLVALILALIVGAFVLGVGLLTSGGYLIWEAFSLMSEGFNIFLMGFGSGLSLIGG
                     AILIYILTGFFAYWSWRLVKACFKWILKRGKRA"
     gene            complement(3518..3844)
                     /locus_tag="AC1PNG_000942"
     CDS             complement(3518..3844)
                     /locus_tag="AC1PNG_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIP6701453.1"
                     /translation="MHFPVPAVLTEFLIMAILESDDSYGYEICQTIKLIANIKESALY
                     PILKRLEQNDFLTTYSQEYQGRMRKYYSLTQLGHEELVRLKDDWDTYTNTINGIIEGS
                     VRHDKN"
     gene            4153..4533
                     /locus_tag="AC1PNG_000943"
     CDS             4153..4533
                     /locus_tag="AC1PNG_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HIP6701454.1"
                     /translation="MKLYVQFMIILVFSFLGEAISTIFHLPIPGSIIGLILLFLALEF
                     KLIRLRHIHTVGNFLLANMTILFLPAAVGIMERFDAIKDYLLPIIIVILGAIFLNILV
                     IGFVVQFVKQKFEGDYIDAEGNHD"
     gene            4526..5221
                     /locus_tag="AC1PNG_000944"
     CDS             4526..5221
                     /locus_tag="AC1PNG_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HIP6701455.1"
                     /translation="MTELFSNPIFGIMLSIAAYLIGMLIYRRFPHPITTPLLVATGLI
                     IVFLKMTGISYKEYYAGGSYLNMLIVPSTVALGIPLYRSFHLMKHHIRSILLGIFVAC
                     IVNTVFTALIAKWFGMDFFLAISLFPKSVTTAMAVGITDKMQGIATVTLVVVVATGIL
                     TSVLGPVFLKLLKIEDPVAVGLALGGTGHAIGTGTAIKYGHTQGAMAGLAIGITGIMY
                     VVISPIVAQIILQ"
     gene            complement(5255..6013)
                     /locus_tag="AC1PNG_000945"
     CDS             complement(5255..6013)
                     /locus_tag="AC1PNG_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HIP6701456.1"
                     /translation="MKRKRLLEFEHSFSAYAVRSILASLYLGLGIVISLYTADKLNHV
                     AEGLGKFSYGLMFGWGLLMILYMNAELGTSNMMYMTVASHRKTIPTKTALRMLATCIL
                     FNFVGAVLVCYLVSLTLPYQHVDAHSYLFEATVAKLSKTPLTQFIEGIFANIVVNIGV
                     FTFLRIKDDAGRLISVIFIIFIFAFLGYEHVIANFSLFSLAFFANGGPVEGMTLLSVL
                     SNFLFSGLGNYVGGGLFIGLLYSWLNNQSKLYVD"
     gene            6101..6946
                     /gene="gla"
                     /locus_tag="AC1PNG_000946"
     CDS             6101..6946
                     /gene="gla"
                     /locus_tag="AC1PNG_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaglyceroporin Gla"
                     /protein_id="HIP6701457.1"
                     /translation="MDVTWTVKYITEFLATALLIIIGNGTVANVDLKGTKGNNSGWIL
                     IAIGYGLAVMMPALMFGNVSGNHINPAFTLGLAVSGLFPWAHVAQYIVAQLLGAMFGQ
                     LVVVAVYKPYFLKTENSNHILGSFSTISALDNGTKESRKAATTNGFLNEFVGSFVLFF
                     GALALTKHFFGSELVGKLIEQGYDKTVAETMTAPYTSGSIAVAHLGIGFLVMALVAAL
                     GGPTGPGLNPARDLGPRIVHALLPKSVLGENKADSKWWYAWVGVCAPIIASIAAVALF
                     KFLYL"
     gene            7075..8607
                     /locus_tag="AC1PNG_000947"
     CDS             7075..8607
                     /locus_tag="AC1PNG_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HIP6701458.1"
                     /translation="MSDVIAFGYGSSRAEMQLKRLNRHGIIAGATGTGKTVTLKVLAE
                     QLSDAGIPVFLSDIKGDLNSLVAANTKEIDPSRLEKTHYLDYSPVGYPVELWDVLGEN
                     GTPIRMTISELGPVLLTRLLGLNDTQESILNIVFSVADERGLLLIDLMDLRAMLNFVA
                     ENAAELSQYYGNIPARSVGAILRSLVVLEQQGGKIFFGEPSLDIADLMRTAEDGRGVI
                     NVLQATQLFNQPTLYSTVLLSLLSELYEVLPEVGDLDKPKMVFFFDEAHVLFKDAPKV
                     LLEKIELIVRLIRSKGVGVFFVTQNPTDIPDSVAAQLGNRIQHGLRAFTPKELKTVAT
                     VAETFRQEGDEDLAKVIQELQVGEAVVSTLQADGTPSFADRVLIYPPKSMLGTVEPSA
                     LLSVINNSPLMEKYADAVNRESAHEQILAMTEAKEAELIKKAEQAEAEKQAEKEAKAA
                     AKMEEKAQKEAAKAAQKTSQPASRKTDSMMDRFTKNLMSQVGREVGRVVTRGIMGMLK
                     GK"
     gene            8719..9327
                     /locus_tag="AC1PNG_000948"
     CDS             8719..9327
                     /locus_tag="AC1PNG_000948"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIP6701459.1"
                     /translation="MTDQLQALLKDYQPQPLGEKRSYAVFLPLVWSDDQWQVLYEIRS
                     ESISQPGEVSFPGGGIDEGETAEEAAIREVIEELDIQQKQIELLGEIDYLVFGRSTIR
                     CFVGRLHLDWQTLQPNDEVARFFTVPLETLLKTAPVYYQLDSQIVPDCDFPFERLRGG
                     VDYPFSHHKRSVPFYENLPENIWGMTAQFTHCFVEIVKSNLS"
     gene            9618..11612
                     /locus_tag="AC1PNG_000949"
     CDS             9618..11612
                     /locus_tag="AC1PNG_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIP6701460.1"
                     /translation="MSKQKVVSSLLLSTVVLGGLAFYSTTTVKAESLEPDVTSVSSSD
                     ATNKLVVSNEEESDFLAEEELEESDAPVETLNEEIKDVTEPSSPEALLQPRAMMSDSM
                     SSGMEEIQVKDEPSDNTAEKVVKQQSDLIQSSSADSFKNGKDKEKLESSVSIDLLSSE
                     NNEIKWKVSFDTSAWSFNYHHGGLYFILPEGLNLKSVKANTNDELFNRFPNKVDSTNN
                     GDGAPFRFFNKEENSDSFNNQWGWSAGQAPNEMINNWKTGNRFSKIYFIDKITDTRQV
                     TYTLTASVTDSNQKSFPLVAVMKSFWYTNSQGTQITSLAGREITLEKEKPLPPKETPA
                     PEAPSTPEKQPESPKEDPKKEDTPEVSPKPEAPTPDAPKQDVPETPDKQPEEMPKVPE
                     APQQDDVQPDAPQVPEVPKQDDVQPDAPQVPEAPKEEVPTPPAPSVPEEQPKETPTPE
                     VPKQDDVQPEAPKSDKVETDKQMPETKQPDMKQPKADDMPKEQKPKADEPKVEQPQME
                     APKKDSEAPKSDKVESDKQLPETKQPDMKQPKADDMPKEQKPKADEPKAEQPQMEAPK
                     KDSEAPKSDKVETDKPMPETKQPDMKQPKADKPEAEKAQMPRTEGMKPESKASMMPKA
                     EAPKATLPNTGEASSAIGWLGGALATLATGLYLFKNKKEE"
BASE COUNT         3479 a         2366 c         2605 g         3266 t
ORIGIN      
        1 tgccaattcc tctcagatca ttgctggggt gggacaattg caatcaggag caagtcaggt
       61 tgaacaactg gtaacagggg ccaatcaatt gcaggcaggc ttggagcaac tcgctagttc
      121 aactagtttg tctgctgaac agtctagtca gatacaggcc ttattgactg gacttcctca
      181 gttgcaggcg gccatcaatc aattaaatga tagcctatca agtatcggag gacttgcagt
      241 tgatacctct actctatcta gcctcttgac ggagatgggg gcccaggctc aaggcttgtt
      301 gacagcagcc caagctgata aaacagccgg cattgaagcc ttgcaagcaa cggctaccta
      361 tcaacacttg ccagctgatc agcaagctga attggttggc gccctccaaa attcaccttc
      421 ggcaacagcg gcagtagccc aaacgatttt gattcaattg tctcagctca gtcaaactct
      481 ttctagctta caaagtcttt ctggcatgga aacacaaatg agccagctac aatcagctgt
      541 cggtcagatc aacacggctg ccaatcaagc attgccaggt gccacaacag ccattgaaaa
      601 cctatcaagt ggcttgagtc aggtcaacac cgccttgaac cagcaagttc taccaggaac
      661 tcaggtgcta acaggtggag taagtcaact tcaaacgcaa ctgtcaagcg gagcgagtca
      721 actcatgtca ggagtaacag cctataccgc tggtgttgcc caattagcag caggtggagc
      781 ccagttggtt gcgaataata gtagtatcca atctgggggt ggccagctaa catcaggatt
      841 ggcaaccttg gcatccaact ccagtcaact tgtcagcggt tcaggacaac tggctttagg
      901 tagtcagcaa ttaattactg gtgcggatca attagctagt ggcggtcaaa ccttgacaag
      961 cggtattact agcctccgca caggtagtga aaccctaacg aattcactca gctcagccag
     1021 tcaacaacta tcagtcgtct ctgtagaaga caaaaatgca caagcagttt ctcaaccagt
     1081 caccttggaa cacagtgacc aagatgatgt gaagaccaac ggagtcggca tggctcctta
     1141 catggtgtct gttgccctaa tggttgcagc cttatcagcg aatgttattt ttgtcaaaca
     1201 cattgataat cgctcctaca agaatcgttg ggactgggcc aaaggaaaac tcttgctcaa
     1261 tgggatcatt gccagtctag cagcagtgat tctgtacggt gttcttcgct tgattggaat
     1321 cgaaccggct catccaatgg caaccttggg attaatcctc ttagcctctt ggaccttcat
     1381 ggccctcgtt acagccttgg tgggctggaa caatcgtttc ggatccttcg caagtttaat
     1441 tatcttgtta ctacaacttg gctcaagcgc aggtacctat ccaattgaac tcagcccacg
     1501 cttcttccaa gtcattcagc tctatcttcc aatgacctat tctgtttcag gtcttcgtca
     1561 aaccgtttct atggttggaa atagtagtca tcaagtttgg atgctgagtc tcttcttagt
     1621 aggctttatg ggactaggtc tcttgattta taatcaaaaa gatgaataat aagcaaacca
     1681 actgctcaat gtgtgaacag ttggtttttg gttgatgtag ttatttgggg gtccctaaat
     1741 tgattgtcgc gttggtggct tcaatggtca gtttgttctt gctacctttg ttttctaggg
     1801 ttagggtctg tagatcgttt tcagtcttga cagggaattt ggtgtactta tcgctagtga
     1861 aaataccgac ttcccctagt cgctctttta agtaagggtc ttggttaacc atctctccca
     1921 aatcattccc atcttcatag tagtaacctt gggcttctac taattttttc ttgtcgatct
     1981 ggttggtgat gagcaagttg ctatctgcca gactcttgtc cgtcacatcc aaaaatgtcg
     2041 tagaggagta ggtgtcggat ggagtgaaac gattatcgcc aagaagagtc gtttgtctgc
     2101 tatccagctg gctatagccc tcgatggtca cattttccaa actggtttca tacaggcgga
     2161 cgctgcttcc ctgtaggttg atgtgcatat cttttagagt ggattgtgtc gcttggaagt
     2221 agcctgcttc aatctgaccg ctgtcaatag tcactttgtc aagagtgaca ccaccagaca
     2281 gatttgcctc tttgatatgg atattctcaa tcgtgaaacc gccaaaatca tagaagtgga
     2341 gattgtgtcc ctctaattta tccagtgtct tccctttagg cacttcaatg gtgacggaga
     2401 agacattgat acgacgctgg gccaagcttt ctcccaaata ctgcatgatt ccctcaatgg
     2461 caaattctag ttcttgtgca gagagagtta agacgccgtc tttctcactg agttttagag
     2521 gtttttggat attgttgtcg gagttggcat aggtcacatg atagttgtta tcactagact
     2581 ctttgatgta gacgctgtgg gggataaaat caattttaat ggtattcaaa tctgtgaagg
     2641 tcttatggac ttccgtcgga gcagcaacct cttctaagcg ggttatacct ccggtgaaaa
     2701 atccaatgcc agctagaatt aagcctgcaa tcaggctgac aaagccgata ataatggcga
     2761 tggtaagttt ctttttcatg ctcgtttacc tcgtttcaag atccatttga agcaagcctt
     2821 gaccagtcgc caagaccagt aggcaaaaaa tccagtcaag atgtagatga gaatagctcc
     2881 gccaatcaaa gacaaacctg acccaaaccc catgaggaag atattgaaac cttctgacat
     2941 aaggctaaag gcttcccaaa ttagatagcc accgctggtg agaagtccga cacccaaaac
     3001 aaaggcaccg acaatgaggg ccaagatgag cgccaccaat cccaatagac tagctaggag
     3061 aatgagcaaa agtggaatgg cgataggcag ggaaatgaca gctaggatag ccaaccaaag
     3121 cgttcgtttt gtttggctaa aattacggaa gtagcgaccg aggaaaaagg cgccgaggat
     3181 aagagtcgca aacagaggaa tgactccgat aaactctccc atgataaaga gtaggaagaa
     3241 agagaagaga cctgtcatac ttaaggctcc cagaccaacc cagagtttcc agttgagttg
     3301 aataggttct tgttgttcct tctcctcctg gatttgcttg ttgagcatgt tgttaatgag
     3361 ttcgctggca gcttcttttg gtgaaccaag ttcttctata atttctgttt cacgctctgg
     3421 tccagcctcg tcaaaatact cattaaagaa gctgatggct tcaaagtatt ctttgtgggg
     3481 cagttttttg agatattttt ctaactgctc catgtattca gttcttgtca tggcggacgc
     3541 tcccttctat gatgccgttt atcgtgtttg tataggtatc ccagtcatct ttgagtcgga
     3601 ccaattcttc gtgacctaac tgagttaggc tataatactt gcgcatacga ccctgatatt
     3661 cttgggaata agtagtcaaa aagtcatttt gctccaatcg ttttaggatg ggatagaggg
     3721 cggactcctt gatgttggca attaatttaa tggtctgaca gatttcatag ccataagaat
     3781 catcggattc caaaatagcc atgatgagaa attctgtcag cactgcgggg actggaaaat
     3841 gcataggacg ctcctttcct ttgttgttag ttaggttcgg ttttgatata tatcgttcag
     3901 ttgtatatat aaaattttgt tatatattaa atatacacct acaaaaataa attgtcaagt
     3961 gattatgtgc aaaataaata aaatagctta agaccaaata gcttaggaca aatttttaag
     4021 gtgtaaacgt tttatagcct tgaaaaatat tttcagagta gtaaacttaa taacagtact
     4081 aacattaagg gaatttctgg actaaaagag aatgacctca gatggattag tagtatttaa
     4141 ggtggtgtaa agatgaaact atatgttcag tttatgatta ttctggtatt ttcattttta
     4201 ggggaagcta tttcgactat ttttcatctt cctattccgg gaagtatcat tggtttaatc
     4261 ctcctattct tagccctaga gttcaaatta attcgtcttc gccacattca tacggttgga
     4321 aattttttgc tggctaacat gaccattctt tttttacctg cagcggttgg gattatggag
     4381 agatttgatg caattaaaga ttatttactt cctattatca tcgtcatttt aggcgccatt
     4441 tttttaaata tcttagtaat tggttttgtt gttcaatttg tgaagcaaaa gtttgaaggt
     4501 gactacattg atgcggaggg aaatcatgac tgaacttttt tccaatccta tttttggtat
     4561 tatgttatca attgcagcat atttaattgg aatgctgatt tatcggagat ttcctcatcc
     4621 aatcacaacc ccactgttgg tagctacagg attgattatt gtctttttaa aaatgacagg
     4681 tatttcctat aaggaatatt atgcaggagg gtcctatcta aatatgctta ttgtgccttc
     4741 aacagtagct ctgggtattc ctttgtatcg ttctttccat ctaatgaaac atcacattcg
     4801 aagtattctt ttgggaattt ttgttgcctg tattgtcaat actgtcttta ctgccctaat
     4861 tgcaaaatgg tttgggatgg atttttttct agcaatctct ctttttccaa aatcagtcac
     4921 cactgctatg gctgttggta ttactgataa aatgcaagga atagcaactg ttactcttgt
     4981 agtcgttgtt gcaacaggaa ttctgacaag tgttcttggt ccagtctttt taaagctact
     5041 aaaaattgaa gacccggtag ctgttggtct tgctttggga ggaacaggac atgccattgg
     5101 aacgggtaca gcgataaaat atggacatac gcaaggagcg atggctggct tggctattgg
     5161 gattacaggc atcatgtatg tggtgattag tccaatagtt gcgcagatta tcttacaata
     5221 aaaaactacg attggaattc caaccgtagt ttttttagtc tacataaagt ttggattgat
     5281 tattcaacca actataaaga agtccgataa agagaccgcc acccacgtag tttcctaatc
     5341 cagaaaagag gaaattgctt agtacactta atagagtcat tccctcaact ggtccaccat
     5401 ttgcaaaaaa ggccagggag aagagtgaaa agttcgcaat gacgtgctca tatcccaaga
     5461 aagcaaagat aaagatgatg aaaataacgg aaatgagacg cccagcgtca tctttgatgc
     5521 gtaagaaggt aaatacaccg atattcacga caatattagc aaaaatacct tcaataaatt
     5581 gggtcaaagg tgtctttgaa agcttggcca ccgttgcttc aaataggtag ctgtgagcat
     5641 caacgtgctg ataaggcaga gtcagtgaga ccaagtaaca aacaaggacc gcaccgacaa
     5701 agttgaaaag gatacaggtt gccaacatcc tgagggctgt tttcgtagga atcgtttttc
     5761 gatggctggc tactgtcata tacatcatgt tggatgttcc aagttcggca ttcatatata
     5821 aaatcatgag tagcccccaa ccaaacatca acccatagct aaatttcccg agtccttctg
     5881 cgacatggtt gagtttgtct gctgtataaa gagaaatgac aatccccaat cctaaataaa
     5941 gactggctag tatcgatcgt acagcgtagg cagaaaaact gtgttcaaat tcgagtaaac
     6001 gttttctttt tatcgaatgg ctttactttg tttattatac ttgataaaat ggttgttgtc
     6061 caaaaaaaat tcaacttttg aaaggaaata taagaaaaat atggatgtta catggacagt
     6121 gaaatatatc actgaatttt tagcgactgc actccttatt atcattggta atggtacagt
     6181 tgcaaacgtt gatttaaaag gcacaaaggg aaacaactct ggttggattc tcattgcgat
     6241 tggctatggt ttggcagtta tgatgccagc attgatgttt ggtaacgttt ccggtaacca
     6301 tatcaaccca gccttcacac ttggtttggc tgtttcaggt ctcttccctt gggctcacgt
     6361 tgctcaatac attgttgccc aattgttagg tgcaatgttt ggtcagttag tggttgtagc
     6421 agtgtataaa ccttacttct tgaaaactga aaattctaac catattcttg gctcattctc
     6481 aactatcagt gcgcttgata acggaacgaa agaaagccgt aaagctgcga caactaatgg
     6541 tttcttgaat gagttcgttg gttcatttgt attgttcttc ggtgcccttg ctttgaccaa
     6601 acatttcttt ggttcagagt tggttggtaa attgattgaa cagggctatg ataaaacagt
     6661 cgctgagaca atgactgctc catatacttc aggttcaatt gcagttgctc acttaggcat
     6721 tggttttcta gttatggcac ttgttgcggc tcttggtggt ccaacaggtc ctggtcttaa
     6781 cccagcgcgt gaccttggtc cacgtatcgt ccatgccctt cttccaaaat ctgttcttgg
     6841 tgaaaacaag gctgattcaa aatggtggta tgcttgggtt ggtgtatgtg ctccaattat
     6901 cgcatcaatt gccgctgttg cacttttcaa attcttgtat ctataatata gagaacccgt
     6961 ttcgtcgggt ttttcttttt ctcttggtat atcctatata aagttgtctg ctctgctatt
     7021 ttcctaaaag ttatgctaca ataaggatag aaaaatcgga aaagaggcta gaatatgtca
     7081 gatgttattg cttttggcta tggaagcagt cgagcggaga tgcagctcaa gcggctcaat
     7141 cgtcatggaa tcatcgcagg tgcgacaggt acagggaaga ccgtgacctt aaaggtcttg
     7201 gcagagcagt tgagtgatgc gggaattccc gtctttctat cggacatcaa gggtgatttg
     7261 aatagtttgg ttgcggccaa taccaaggag attgatccta gtcgtctaga gaagactcat
     7321 tatcttgact atagcccagt cggttatcca gttgagttgt gggatgtctt gggggaaaat
     7381 gggactccta tccgcatgac tatttcagag ctgggacctg tcctactgac acgtttgttg
     7441 ggcttgaatg atactcagga gtccatcttg aatatcgttt tcagcgtggc agacgagcgt
     7501 ggccttctct tgattgattt gatggaccta cgggctatgc tcaattttgt ggctgaaaat
     7561 gcggcagagc tgagccaata ttatggaaat attccagctc gttcagttgg tgccatcttg
     7621 cgtagcttgg ttgttttgga gcaacaaggt ggtaagattt tcttcggtga gccaagcctt
     7681 gatattgctg atttgatgcg tacggcagaa gatggccgtg gggttatcaa cgttctccaa
     7741 gctactcagc tattcaatca accaactctt tattcgacag tcctgcttag cctcttatca
     7801 gaactctatg aagtcttgcc tgaggtgggc gatttggaca agcctaagat ggtcttcttc
     7861 tttgacgagg ctcatgttct cttcaaagat gctccaaaag ttcttcttga aaagattgaa
     7921 ttgattgtcc gtttgattcg ttcaaaaggt gtaggtgtct tctttgtaac gcagaatccg
     7981 acggatattc ctgatagcgt ggcagcccag ttgggcaacc gcatccaaca tggtcttcgc
     8041 gcctttacac caaaagagct taagacagtc gcgacagttg ccgaaacctt ccgtcaagaa
     8101 ggtgatgagg atttggccaa ggtcattcag gagttgcagg taggtgaagc ggttgtgtct
     8161 actctgcaag ctgacgggac accaagcttt gcggatcggg ttttgattta tccaccaaag
     8221 agtatgctgg gaacggtgga gccaagcgcc ctcttgtcag ttatcaataa ttctcctttg
     8281 atggaaaaat atgctgacgc ggtcaatcgt gagtcagccc atgagcagat tttagccatg
     8341 acagaggcta aagaagccga actcattaaa aaagcagagc aggccgaagc ggaaaaacag
     8401 gcagaaaaag aagctaaggc agccgcaaaa atggaagaga aagcccagaa agaagcagct
     8461 aaagctgcac aaaaaactag tcagccagcc agtcgcaaaa cagattccat gatggatcgc
     8521 tttaccaaga acctgatgag tcaggtcgga cgggaagttg ggcgcgtggt aactcgtggc
     8581 attatgggca tgttaaaagg gaaataaacg actagactga gaaggaaact tctcagtttt
     8641 ttatctagac aaagttcttg ccagatgcca caaaattcgt taaaataaac gtaaatcact
     8701 agttagtaag gagttaccgt gactgatcaa ttgcaggcat tattgaaaga ctatcagccc
     8761 cagccattag gtgaaaaacg ttcttatgca gttttcttgc ctctagtatg gtcagatgat
     8821 cagtggcaag ttctatatga aattcggagc gagtccattt ctcagccagg agaagtttcc
     8881 tttccaggtg gcggaattga tgaaggcgag acagcagaag aagctgccat tcgtgaggtt
     8941 atcgaagaac ttgacattca acagaaacaa attgagttgc tgggagaaat tgattatctc
     9001 gttttcggtc gctcgacgat tcgctgtttt gtcggtcgac ttcacttgga ctggcagacc
     9061 cttcaaccca atgatgaagt ggctcgattc tttacagttc cactagaaac cttgctgaaa
     9121 acggcccctg tctattatca actggattca cagattgttc cagattgtga ctttcccttt
     9181 gagcgtttga gaggtggggt tgactatcct ttcagtcatc acaaacgctc cgttcccttt
     9241 tatgaaaatc taccagaaaa tatctgggga atgaccgctc aatttaccca ttgttttgtg
     9301 gaaatagtga agagtaatct atcgtgaaat gataggttat ttttaggagg aaaaaccagc
     9361 ctttgcgata tgtatcagcg aaaaaaatgg agataaagct caatgtgagt aatgtataga
     9421 aaaacacaaa aaaaacagat gcatggcaga taaaagatag ctatggctgg tatacttggt
     9481 acggtccata tttttaagta ccatcatcta tccaggaatg cgacaatatt catgtaaacg
     9541 cattcagtgg cttgttgtca tttccatgta gatgagtggg aaaatgtata aatctatatt
     9601 ttaagaagga gccaattatg agcaagcaga aagttgtgtc cagtttatta ttaagcacag
     9661 tcgtattagg gggattagcc ttttattcaa cgacaacggt taaagcagaa tcgctagaac
     9721 cagatgttac atcagttagt tcaagcgatg ctactaataa actggtagta tcaaatgaag
     9781 aggaaagcga ctttttagcc gaagaagagc ttgaagagag cgatgctcca gtagaaactc
     9841 taaatgaaga aatcaaagat gtcacagagc catcttcacc tgaagcactt ctccaacctc
     9901 gtgcgatgat gagcgattct atgagtagcg gtatggaaga aattcaggtg aaagacgagc
     9961 cttcagataa tacggcagaa aaggtagtga agcaacagtc ggacctaatt caatcatcga
    10021 gcgcggactc ctttaagaat ggaaaggaca aagagaaact cgagtcttca gtgtctatag
    10081 acttgttgag ttcagaaaac aatgagataa aatggaaagt aagttttgac acaagtgcat
    10141 ggtcttttaa ttatcatcat ggaggtcttt attttattct tccggaaggt ttgaatttaa
    10201 agagtgttaa agctaatact aatgatgaat tatttaatcg attccctaat aaggtagata
    10261 gtactaataa tggtgatgga gcaccattcc gtttctttaa taaagaagaa aacagcgata
    10321 gctttaataa tcaatggggg tggtcagcag ggcaagctcc taatgaaatg attaataact
    10381 ggaaaacagg aaatcgtttt tctaagattt actttataga taaaataaca gatactagac
    10441 aggtaaccta tactttaact gctagtgtga ctgattcaaa tcaaaaatca ttcccacttg
    10501 tagcagtgat gaagagcttt tggtatacaa actcgcaagg aacgcagatt acttcattag
    10561 ctggtcgtga gattaccctt gagaaagaaa aacctctacc acctaaagag actccagcgc
    10621 cagaagctcc gtcaactcct gaaaagcaac cggaatctcc aaaggaagat cctaagaagg
    10681 aagatactcc ggaggtttca ccaaaaccag aagcgccaac accagacgca ccaaaacagg
    10741 acgttccaga aactccggat aagcaaccag aagaaatgcc taaagttcct gaagcaccac
    10801 aacaagacga tgtccaacca gacgctccac aagtaccgga agtaccaaaa caagacgatg
    10861 tccaaccaga cgctccacaa gtaccggaag caccgaagga agaagttcca accccaccgg
    10921 ctccatcagt accagaagag caaccaaaag aaactccaac accagaagtt ccaaaacaag
    10981 acgatgttca accggaagca ccaaaatctg ataaagtaga aactgacaaa caaatgccag
    11041 aaactaagca accagatatg aagcaaccga aggcagacga catgcctaag gaacaaaaac
    11101 cgaaagccga cgagcccaag gtagagcaac cacaaatgga agctcctaag aaagattcgg
    11161 aagcaccaaa atctgataaa gtagaatctg acaagcaatt gccagaaact aagcaaccag
    11221 atatgaagca accgaaggca gacgacatgc ctaaggaaca aaaaccgaaa gccgacgagc
    11281 caaaggcaga gcaaccacaa atggaagctc ctaagaaaga ttcggaagca ccaaaatctg
    11341 ataaagtaga aactgacaaa ccaatgccag aaactaaaca accagatatg aagcaaccga
    11401 aggcagataa accagaagca gagaaagctc aaatgccacg gacagagggt atgaagcctg
    11461 aatctaaggc ttcaatgatg ccaaaagcag aggcaccaaa agcaactttg ccaaatacag
    11521 gcgaagcaag tagcgcaata ggctggctag gtggtgcttt agccactctt gccacaggct
    11581 tgtatctatt caaaaacaaa aaagaagaat agtcattatt gaataagata tgagaacttc
    11641 cctcttctaa gttcataaaa gactccagat catcgctgta gaagccttgg tttggagtct
    11701 ttttgttaaa tattct
//
LOCUS       DBNFEG010000051        11666 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.051, whole genome shotgun sequence.
ACCESSION   DBNFEG010000051 DBNFEG010000000
VERSION     DBNFEG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11666
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.051"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            180..434
                     /locus_tag="AC1PNG_000950"
     CDS             180..434
                     /locus_tag="AC1PNG_000950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701461.1"
                     /translation="MNKRTKKKYKPFKELWDCMEWVMFRLNRHVTRLDSLENRLENMR
                     VIDSANIQTINRKFEEYEKRVETLEREVKQLKKPWYKRRK"
     gene            434..664
                     /locus_tag="AC1PNG_000951"
     CDS             434..664
                     /locus_tag="AC1PNG_000951"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701462.1"
                     /translation="MAEVVTMDELAKNKERGVGCRGILKAELETVTNIESIVIVSLNK
                     NNQINVSYSRNSSLEALGMLDIGKAILLDEMQ"
     gene            748..2163
                     /locus_tag="AC1PNG_000952"
     CDS             748..2163
                     /locus_tag="AC1PNG_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HIP6701463.1"
                     /translation="MVTKTKTRLGNQRPTQSVTLHYAESLAHEAIELYQKTGRSCYPW
                     QVNLLEHIMGIDTEGLWVHQKYGYAIPRRNGKTEDVYMLELWALHHGLKILHTAHRIS
                     TSHSSFEALKKLLEDMGYVDDVDFVSNKAKGQERIEFKSTGSVIQYRTRTANGGLGEG
                     FDLLIIDEAQEYTAEQESALKYTVTDSDNPMTVMCGTPPTMVSTGTVFTNYRTKVLAG
                     KSEYSGWSEWSVEDIKDVNDTDSWYLTNPSLGYHLTERKIKAEIGDDELDHNIQRLGY
                     WPSFNQKSAISENEWKRLEVEAVPELTGKLFVGIKYGQDGANVSMAIAARTDDKKIFT
                     EVIDCQSVRNGMQWIINFLQTADIEKVVVDGANGQTLLANEMKDFKLRKPIMPTVSEI
                     ITANSVWEQAIVQETLRHANEPSLTDVVTNCDKRNIGSNGGFGYKSLYDDRDISLMDS
                     ALLAHWACYMTKPKRKQRISY"
     gene            2244..2708
                     /locus_tag="AC1PNG_000953"
     CDS             2244..2708
                     /locus_tag="AC1PNG_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid assembly scaffolding protein Gp46 family
                     protein"
                     /protein_id="HIP6701464.1"
                     /translation="MMSFKPIETQEELDRIIGERLGRQKEKYADYDQLKNRVSELEKE
                     NGVLKSAAESTKASTSDLEKQIAELQGQVKTYEGKDLRLRVAVANGLPIELADRLAGD
                     DEEAIKADAERLASFMKPTEPTPPMASTEPNVESGSAEDEALKGMLRKMRGE"
     gene            2712..3602
                     /locus_tag="AC1PNG_000954"
     CDS             2712..3602
                     /locus_tag="AC1PNG_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIP6701465.1"
                     /translation="MATLRSGDMFPEQTVKDIFSKVKGHSTLAKLSTQEPIPFTGTDT
                     FVFNLEGNAEIVGEGEPSNAGNATMKPKVIKPVLITYQARVSEEFVKCSEEKQLNYLK
                     SFIDGLAKKVAQAIDIASFHGLEPKSMTAASFKETNSFDGLITGNVVEYDANTIDECI
                     DAAVATVTANEGEVNGIALSPAAGAALGKIKVNGVVQYPEYRFGQNPEAFYGMKSDVN
                     KTLATVASGAKKDFAIVGDFENALKWGYAENIPLEIIEFGDPDGAGRDLKRYREVCLR
                     TEVYAGWGILDPESFARVEG"
     gene            3604..3822
                     /locus_tag="AC1PNG_000955"
     CDS             3604..3822
                     /locus_tag="AC1PNG_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HeH/LEM domain-containing protein"
                     /protein_id="HIP6701466.1"
                     /translation="MEYINKKTKATVETDAKLAGDWVPASEFEKAIENLTVPQLKGKL
                     DELGIEYNKKAQKAELLELLEAAKSEME"
     gene            3826..4221
                     /locus_tag="AC1PNG_000956"
     CDS             3826..4221
                     /locus_tag="AC1PNG_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015971737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp19/Gp15/Gp42 family protein"
                     /protein_id="HIP6701467.1"
                     /translation="MEPFATIEDLSTLWRGLKPAETKRAEALLKVVSDSLRVEAEKVG
                     KDLDKMVEAKSYFATVVKSVTIDVVARTLMTSTDQEPMTQYSESALGYSVSGSYLVPG
                     GGLFIKDSELKRLGLKKQRFGVIELYDKD"
     gene            4208..4546
                     /locus_tag="AC1PNG_000957"
     CDS             4208..4546
                     /locus_tag="AC1PNG_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701468.1"
                     /translation="MTRINGRPIVLIDKQVIGKDSFGHPKTADVEIEVENVLIAPATT
                     EDITNQINLTGKKVVYTLAIPKGDMHDWTNKEVRFFGQRWRTVGEPLEGLEHLIPLGW
                     NKKVQVERYG"
     gene            4539..4778
                     /locus_tag="AC1PNG_000958"
     CDS             4539..4778
                     /locus_tag="AC1PNG_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701469.1"
                     /translation="MARMKFKLNRAGVRDLLKSPEMQAVLTDKANAIRNRAGDGYESD
                     IYVGKTRANAMVYADSIKAKRDNKKHNTLLKAVKS"
     gene            4775..5110
                     /locus_tag="AC1PNG_000959"
     CDS             4775..5110
                     /locus_tag="AC1PNG_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701470.1"
                     /translation="MIEVITLNFLTEHLSVPVYTEHEEEMPDSFVVFEKTSGGKKNHL
                     NQATLAIQSYGQSLEEAAFLNEEVKQAVEKMVELPSISRVELNSDYNFTDTETKRYRY
                     QAVVDFIYF"
     gene            5126..5713
                     /locus_tag="AC1PNG_000960"
     CDS             5126..5713
                     /locus_tag="AC1PNG_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIP6701471.1"
                     /translation="MADAKLVSSAKPDIAGAISSAPTGTSLPTNATTKLNTAFKNLGY
                     ISEDGLTNEDTRESEELKAWGGDVVDTPQTGKSDKFTYTLIEVLNVDVLKEVYGPENV
                     KGDLESGIAVEVNSKELPTHPLVVDMLLKNGAKKRIVIPNAKVLEVGEITYADSDLAG
                     YETTIQALPDSKGNTHYEYIKGASESTGTSSPSSS"
     gene            5724..6002
                     /locus_tag="AC1PNG_000961"
     CDS             5724..6002
                     /locus_tag="AC1PNG_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701472.1"
                     /translation="MFEVKTSTGLVLSIDQDRLENYELFEAIAAEESGDSGAMIRIVN
                     LLLGDEAKKLKDHVRTEKGLVPISALGAEIKDIFEQVKDLKNSQSSPE"
     gene            5999..6382
                     /locus_tag="AC1PNG_000962"
     CDS             5999..6382
                     /locus_tag="AC1PNG_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5361 domain-containing protein"
                     /protein_id="HIP6701473.1"
                     /translation="MIAVDEDALVCDLAETYGIYDYRQLPINRVAVFACGLSESSRIK
                     KVLSGQKEDLDTLLLAGIYDTVRLLFWAKTKDGQAGRNRPNSVAQALVGSKAEREERV
                     FSSDEEFERAMRALEIEIGGEEHGD"
     gene            6372..8954
                     /locus_tag="AC1PNG_000963"
     CDS             6372..8954
                     /locus_tag="AC1PNG_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIP6701474.1"
                     /translation="MATDLGSAYVQIVPSAKGISGSISKLLGGEVDSAGRSAGSSLGA
                     SLVSALSGALAAAGIGKIIGSALGAGADLQQSFGGLDTIYDGAQESAKQFAKEAYKAG
                     ISANTYAEQAVSMGASLKQALGGDSTKAINMANVAIMDMTDNAAKMGTDIGIIQQTYQ
                     SLARGNYAMLDNLKLGFGGTKAEMERLLTTAEGLPSAMGRKFDISNYADVVEAIHLVQ
                     ESMGIAGVAAAEAQNTYSGSLAAMKASWENTLAGLSLGENIAPQLQALATTTSNFLFG
                     NFFPMVGNIFKGLPTMLGTILGDGLGRVFGGEVSGKVMGELNKLNEIILTFYDMTFGS
                     LSEKDNIDMLEKVGFSKETASKIVSLSSQIGSVITSFYDMIFGSLSEKDNIDFMAQMG
                     VDEGTATTIVNFANTIRTGFEGVWSTVQTLFGQVPGFFSSIIGAIGPIITTIMDGISK
                     LDFSGIQTLIESVLPAVQAGFQTFMSIVSPAIDSVVQSFVSMWNAAQPLITILSGALM
                     PVFQILGSFLGGVLKGVLSGVSFAFDALKVAIEFLTPVVDFLVQALNFVQPVLSTIAE
                     WIGVAIGMFGNLGTAGQGLSAFIKSAWTNIQSAIQTAGNIIRTVIDWIKLAFSGAGNA
                     VGVLKNVFSLAWMGIQDAIQVAKGVIDGVISGIKGAFTSFQSVVSSVGGAVNGVITNV
                     ISTIRGIANIDLSGAGSAIMNGFLGGLQSAWEGVKSFVGGIAGWIAENKGPISYDRVL
                     LKPAGLAIMQGLNTNLQVGFKDVMGTVSGMAGAIAKPFENQSLAYDMTSSASVDVRRN
                     LLSSTGDLAGNDGGNSLATRLANIERFLSALVDKELAVYLDGEKLAQNSYMHQGAIMA
                     REGI"
     gene            8954..9649
                     /locus_tag="AC1PNG_000964"
     CDS             8954..9649
                     /locus_tag="AC1PNG_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIP6701475.1"
                     /translation="MNYMIINGLDTSTLADCHVLDFGKAQTSVERSEQVEVFGANGQL
                     HVSEGAYDGYNRTFIITLRHLADAMRLIEVFQSENNTVEFGYLRDSIFYCNLVSSSYV
                     PLGPHRWKVEITVSMHPFRYVKNPADVVLTSSGSVQNPGTVYSEPIIIIEGSGRVTLT
                     IGQQLMELELDTRATIDCRHKRQNIYDKNGAVKNTIRKRGPFFEIAVGRSGIATSGTV
                     SKITIKGNWRYKV"
     gene            9646..>11666
                     /locus_tag="AC1PNG_000965"
     CDS             9646..>11666
                     /locus_tag="AC1PNG_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HIP6701476.1"
                     /translation="MIYLKDGNIPLNLAYDDDIVQEANSTYQLSFKFPLTDGKWNLLR
                     REVFLLADDLHGEQEFFIFEVKKAKGHVQVYAKQVATLLNYYSINSISVDRVPGQTVM
                     TALAGSVKRPCPFTFFSDILDRHTFNESNVSVMAALAKDKHSIVGQWGGDLVRDKYQV
                     KLLKNGGIENESLFIYKKNLSSYEESENINNLKTRLHLKKTIKGQSEGEADRVIAVTV
                     DSPLIGQYRQIYEADIEVNDQDVTDVASLTAYGKRYFSSTLCDLVENSINLDVKGKSD
                     VSVKMFDMVSVFHERFDVDLRLKISSYHFGPMSKRLKSIGFGKVSQSFGSTVASMVAG
                     SVDKATGRLSASFEQKLQKEIDNANRHFDAEFDKRVESINDGIEQAQAEAERYADAIK
                     QEIDTEIAQVNQSMQAQSEEHDRQVADILSKTQSVESLANQAKSDAASALARANQVKT
                     EAIADARAQVATVSQALNTAKSELQTAIASADQKARDSQASATALRNDLNLQASKILE
                     QARAQTALTNRVTTVETLADGTRSTVAELSKTVNKATGDITSVTARTKTVEDTLSQTR
                     TQYEALTQTVNTQTGQIESINRKTADLQSGIDGVTERFENLRVGGTNLFKNSDFSQGE
                     KNWHKLPEIHNEATGKYVRLPARLWTMAQFVEVEQGEDYVISLYAKKIS"
BASE COUNT         3420 a         2165 c         2954 g         3127 t
ORIGIN      
        1 ctaaaacagg tgtcttgcca gtttttgttg cgacgtctag cgtcaaaaga gttctcgact
       61 taacaagata cctctacttt atcataaaga aaaagtagtg agtactttct cccgtcggag
      121 atttcctcac tactaatctt agtatgtttt tgttgtagta aaacagaaag gaggtcgcta
      181 tgaataagcg taccaagaag aaatacaaac cattcaaaga attatgggat tgtatggaat
      241 gggttatgtt tagattgaat agacatgtta ctcgactgga tagcttggag aatcgtttgg
      301 aaaatatgag agttattgat tccgcaaata ttcagaccat caatcgtaag tttgaagagt
      361 acgaaaaacg ggttgaaaca ttagaacgtg aagtcaaaca gcttaagaaa ccatggtata
      421 aacggaggaa gtgatggcag aagtggtcac aatggatgaa ttagccaaaa acaaagaacg
      481 aggagttggt tgccgtggta ttctcaaggc agagctggag acagttacca acattgaatc
      541 aattgttatc gttagcttga acaaaaataa tcagattaat gtttcttata gtcgcaatag
      601 cagcttagag gcattaggta tgttggatat tggtaaagct attttgttag acgaaatgca
      661 ataggaaccg tgcggaatcc catacggttt tcgatttgcc ctgtcgcatg gcgttaaact
      721 aggcaactac gattgaaagg atatgctatg gttactaaga cgaaaacaag gcttggcaat
      781 cagcgtccaa ctcaatcggt aactttacat tatgctgaaa gtctggccca tgaggctata
      841 gaactttatc agaaaacagg tcgcagctgt tatccttggc aggtcaattt gcttgagcat
      901 atcatgggaa ttgataccga aggcctttgg gtacatcaga aatacggcta tgctattcca
      961 cggcgaaacg gtaagacaga agacgtctat atgcttgagt tgtgggcttt gcaccatgga
     1021 ttgaaaatct tgcacactgc acacagaatc agcacgtctc attcttcttt tgaagcattg
     1081 aaaaaacttc tggaggacat gggatacgtg gatgatgttg actttgtttc taacaaggca
     1141 aaggggcagg aacggattga gtttaaatct actgggtcag tgatacaata ccgaactcgg
     1201 acagccaatg gcggtcttgg tgaaggtttt gatttgctga ttatagatga agctcaggaa
     1261 tacacagccg aacaagaatc agctttgaaa tacacggtaa cggacagtga taaccctatg
     1321 acggttatgt gtggaacacc accaaccatg gtatcaacag gaactgtctt taccaattac
     1381 cgaacaaagg ttttagccgg taaaagcgaa tactcgggtt ggtctgagtg gtcagtcgaa
     1441 gacatcaagg atgtcaacga tactgactcc tggtatttga ccaatccgtc tcttggttat
     1501 cacttaaccg agcgtaaaat caaggctgaa attggagatg acgaacttga ccataatatt
     1561 cagcgtttag gttactggcc aagctttaac caaaaatctg ctatctctga aaatgagtgg
     1621 aagaggcttg aggttgaggc ggtgccagag ctaacaggaa aactattcgt tggtatcaag
     1681 tatggtcaag atggggcaaa tgtgtctatg gctattgcag cacgaacgga cgacaagaaa
     1741 atcttcactg aagtgattga ctgtcaatct gtccgaaatg gcatgcagtg gatcatcaac
     1801 tttttgcaaa ccgcagacat tgagaaggtt gtggttgatg gagcgaacgg gcaaacacta
     1861 ttagcaaacg aaatgaaaga tttcaaactt aggaagccaa tcatgccgac agtcagcgaa
     1921 atcatcacgg ccaactctgt ctgggaacaa gctattgttc aggaaactct aagacatgct
     1981 aacgagcctt ctttgactga tgttgtgacc aattgtgata aacggaatat aggctcgaac
     2041 gggggatttg gttacaagtc cctttatgat gaccgagaca tcagcttgat ggatagtgct
     2101 ttgctagcgc actgggcttg ctacatgacg aagcctaaaa gaaagcaaag aatcagttat
     2161 taaaagtagc atccagaaat tgggtgcttt tttaatgcac aaaaattacc gaacgcacgg
     2221 gaaaatgcgg agaaaggacg ttaattatgt catttaaacc aattgaaaca caggaagaac
     2281 ttgatcgcat tatcggtgaa cgcttagggc gtcaaaaaga aaaatacgct gattatgatc
     2341 agttgaaaaa tcgtgtttca gaacttgaaa aagaaaacgg tgtgttgaaa tctgcagctg
     2401 aatcaactaa agcaagtaca tctgatttag aaaaacaaat tgcagagctg caaggtcaag
     2461 tcaaaactta cgaaggcaaa gacttgcgct tgcgtgtagc agttgccaat ggcttaccga
     2521 ttgaacttgc tgaccgtctt gcaggagatg atgaggaagc catcaaggca gatgccgaac
     2581 gtttggctag ctttatgaag ccgacagaac caacaccgcc aatggcatca acggagccga
     2641 atgtagaaag tggctctgca gaagatgaag ctctgaaagg gatgcttcgt aaaatgagag
     2701 gagaataaca gatggcaaca ttgcgatcag gggatatgtt cccagaacaa acagtaaaag
     2761 atatttttag caaagtaaaa ggacactcta cactagctaa actttcgaca caagagccta
     2821 ttccattcac aggaacagat acatttgttt ttaacttgga aggcaatgct gagattgttg
     2881 gtgaaggcga gccatcaaat gcaggcaacg ctacaatgaa gcctaaagta attaaaccag
     2941 ttttaattac ttatcaggcg cgtgtatcag aagaatttgt taaatgctca gaagaaaaac
     3001 agcttaatta cctcaagtcg ttcattgatg ggcttgccaa gaaagttgct caggcaattg
     3061 atattgcctc attccatgga ttggaaccaa aatcaatgac ggcagcttcg tttaaagaaa
     3121 caaactcatt tgatggcttg attactggaa atgtcgttga atatgatgca aacacaattg
     3181 atgaatgtat tgacgcagcg gtagctacgg ttacagcgaa tgagggcgaa gtcaacggta
     3241 ttgcactttc cccagcggct ggtgcagcac ttggaaaaat caaagtcaat ggtgttgttc
     3301 aatatcctga ataccgtttt ggccaaaacc ctgaagcatt ctacggaatg aaatcagatg
     3361 tcaataaaac attagctaca gttgctagcg gagctaagaa agatttcgcc attgtcggtg
     3421 actttgaaaa tgcacttaaa tggggatatg ctgaaaacat tccgcttgaa attattgaat
     3481 ttggtgatcc agatggcgct ggtcgcgact tgaaacgcta ccgtgaagtt tgcttgcgta
     3541 ctgaagtata tgcaggttgg ggaattctag acccagaatc atttgcacgt gtggagggat
     3601 aacatggaat atatcaacaa aaaaacaaaa gcaactgttg aaacagatgc aaaactagct
     3661 ggagactggg tgcctgcttc cgaatttgaa aaagccattg aaaatctaac tgttccacag
     3721 cttaagggaa aacttgatga actcggcatt gagtacaaca agaaagccca gaaggcagaa
     3781 cttttagaat tgctggaagc tgctaaatca gaaatggagt agtccatgga accattcgct
     3841 acaatcgaag acttgtccac attgtggcgg ggactaaaac cggccgaaac caagagagca
     3901 gaggctcttt taaaagtcgt ctccgactct cttagggttg aggctgagaa agtaggtaag
     3961 gacctcgaca agatggtcga agcaaagtct tatttcgcca cagtggtcaa gtctgtgact
     4021 attgatgttg tcgcacgtac ccttatgaca tcgactgacc aggaacctat gacgcagtat
     4081 tccgagtcag cccttggcta ctctgtctct gggtcttatc tggtccctgg tggcggtctc
     4141 tttatcaaag actcggaact taaacgctta ggtctgaaaa agcaaagatt tggggtgatt
     4201 gaactttatg acaaggatta atggtagacc gattgtcttg attgacaaac aggtcattgg
     4261 caaggattcg tttggtcatc caaagactgc agatgtggag attgaggttg agaacgtctt
     4321 gattgcgcct gcaacaaccg aggatattac caatcaaatc aatcttacag gtaaaaaagt
     4381 tgtctatact ctcgctattc ctaaagggga tatgcacgac tggacgaaca aggaagttcg
     4441 attttttggc caacgatgga gaacggttgg cgagcctttg gaagggcttg agcatttgat
     4501 tccgctcggg tggaataaga aagtgcaggt ggaacggtat ggctagaatg aaatttaagt
     4561 taaaccgtgc tggtgtccgt gacttgttaa agtcgcctga gatgcaggca gttttgactg
     4621 acaaggcaaa tgctatccga aaccgtgcag gcgatgggta tgaatcggat atctatgttg
     4681 gtaagactcg tgctaatgct atggtttatg cggatagtat taaagctaag cgggacaaca
     4741 agaagcacaa taccttgtta aaggcggtaa aatcatgatt gaagttatca cgttgaactt
     4801 tttgaccgaa catctttctg tgcctgtcta tacggagcac gaggaagaga tgccggatag
     4861 ctttgtggtc tttgaaaaga ccagcggtgg gaaaaagaac catctgaacc aagcgacttt
     4921 agcgatacaa tcttatgggc agtctttgga agaggctgcc tttttgaacg aagaagtcaa
     4981 acaagctgtt gagaagatgg tggaattgcc gtctatcagc agggttgagt tgaactcgga
     5041 ctataatttc acagatacgg aaaccaaacg ctatcgctat caagcggtag tggattttat
     5101 ttatttttga aaaggagaaa attgaatggc agatgcaaaa cttgtgtcat cggcaaagcc
     5161 tgatattgct ggggcgattt cctcagctcc aacgggaacg agcttgccga ccaatgcgac
     5221 tactaagcta aacacagctt ttaaaaactt aggctacatt tcagaggatg ggttgaccaa
     5281 cgaggacact cgtgaatcgg aagaactgaa agcctggggc ggtgatgtgg ttgatactcc
     5341 tcaaacagga aaatcagaca aatttaccta cacactcatt gaggtgttaa atgtggatgt
     5401 cttaaaagag gtctatggtc ctgaaaatgt gaaaggcgac cttgaatcag ggattgctgt
     5461 ggaggtcaac tctaaggaat tgcctacgca tccgttggta gtggatatgc tgctgaaaaa
     5521 tggtgcgaag aagcggattg tcatcccaaa tgctaaggtg ctggaagttg gggagattac
     5581 ctacgccgac agtgacttgg ctggttatga aacaactatc caagctctgc cagatagcaa
     5641 aggcaataca cactatgagt acattaaggg agctagcgag tcaacaggaa caagctcgcc
     5701 atcgtcatcg taaggaggtt tgaatgtttg aagtaaaaac tagtacaggt cttgtgctta
     5761 gcattgacca agaccgtttg gaaaactatg agctttttga ggcaatcgct gctgaagaat
     5821 ctggagacag tggtgccatg attcggattg tcaatttatt actcggtgac gaagcgaaga
     5881 aactcaagga ccatgtccgt acagaaaaag ggctggtgcc aatttctgct cttggtgctg
     5941 aaattaagga cattttcgaa caagtcaaag acttaaaaaa ctcgcaatcc tcgccagaat
     6001 gattgcggta gatgaggatg ctcttgtctg tgatttggct gaaacctatg gcatatatga
     6061 ttatcgacag ctacctataa atcgggtagc tgtttttgct tgtggtttaa gtgagtcatc
     6121 acggattaag aaggtcttgt ctggtcagaa ggaagacttg gatactctgc tgcttgcagg
     6181 tatctatgat acggttcgct tactcttttg ggctaaaact aaggacggac aggccggacg
     6241 gaatcgtcca aactctgtcg cccaagcctt ggtaggttcg aaagcggaac gtgaagagag
     6301 ggtcttttca tctgatgagg agtttgaacg tgctatgcgt gcgctagaaa tagagattgg
     6361 aggtgaggag catggcgact gatttgggtt ctgcttatgt gcagatagtt ccgtctgcta
     6421 aaggcattag cgggtcgatt tcaaaattat tgggtggcga agttgatagc gctggtaggt
     6481 cagctgggtc aagtcttggc gcttcactcg tctctgcttt aagtggcgca cttgcggcag
     6541 caggaattgg gaaaattatc ggttcggctc ttggagctgg tgctgatttg caacaatctt
     6601 ttggtggtct ggatactatc tatgatggtg ctcaagagtc tgctaagcag tttgcaaaag
     6661 aggcctacaa ggctggaata tcggcaaaca cctacgcaga gcaagctgta tccatgggtg
     6721 ccagtctgaa acaagctctt ggtggtgatt cgaccaaggc aatcaacatg gcaaacgtgg
     6781 ctatcatgga catgacggac aatgcggcta aaatgggtac cgatatcggt atcatccaac
     6841 agacctatca aagtttagcc cgtggcaact atgccatgct agataatctt aaactcggct
     6901 ttggtggcac aaaggctgaa atggagcgat tgttgacgac tgctgagggc ttgccgtctg
     6961 ctatggggcg caagtttgat atcagcaact atgcggatgt tgtcgaagct atccaccttg
     7021 tgcaagaaag catggggata gctggagttg cagctgccga agctcagaat acttattcag
     7081 ggtccttagc tgctatgaaa gcgagttggg aaaatacact ggcaggcttg tccttgggcg
     7141 agaatatagc tccgcaatta caagccttgg caacgacgac ttctaatttc ttatttggaa
     7201 atttctttcc catggttggt aatatcttca agggtttgcc gacaatgctt gggactattc
     7261 ttggagacgg tttaggtaga gtctttggcg gagaagtgtc tgggaaggtc atgggcgagt
     7321 taaataagct aaacgaaatc atcttgacat tttacgatat gacttttggt tccttgagcg
     7381 agaaagacaa tatcgatatg ctcgaaaagg ttggctttag taaagaaaca gcttcaaaga
     7441 ttgtgtcttt atccagtcaa attgggtcgg tcattacgtc gttttatgac atgatttttg
     7501 ggtcgttgag tgagaaagac aatattgatt ttatggctca aatgggggtc gatgaaggta
     7561 ctgcaacaac gattgtcaat tttgcaaata caattcgaac tggctttgag ggtgtttggt
     7621 ctactgtaca gacattattt ggacaagttc ctggtttctt ctcttcgatt attggagcga
     7681 ttggaccaat cataacgact atcatggatg ggatttcaaa actggacttt tcaggtatcc
     7741 aaacgttgat agagtctgtt ttgccagcag ttcaagcagg tttccagacc ttcatgagca
     7801 ttgtcagtcc tgccattgat tcggtcgttc aatcgtttgt gtctatgtgg aatgcggcac
     7861 agcctttgat aaccattttg agcggtgctt tgatgcctgt ttttcaaatt ctggggtcat
     7921 tccttggagg agtcctcaaa ggtgtcttgt cgggtgtgtc gtttgcattt gatgctttga
     7981 aagttgctat tgagttcttg acacccgtag tcgatttttt ggtacaggct ttgaatttcg
     8041 ttcagcctgt gctgagcacg attgctgaat ggattggtgt ggctatcggt atgtttggta
     8101 atctgggtac agctgggcaa ggtttgagtg cctttatcaa gtcggcttgg accaatattc
     8161 aatcagctat tcagacggct gggaatatca tccgaacggt cattgactgg atcaagttgg
     8221 ctttttctgg tgctggaaat gctgttggtg tgcttaagaa tgtcttttcc ttggcttgga
     8281 tgggtattca agatgccatt caagttgcga aaggggtcat tgatggtgtt atctctggca
     8341 tcaagggtgc ttttacaagt tttcaaagcg ttgtatctag cgtcggcggt gctgtcaatg
     8401 gggttatcac taatgttatt tctaccatta gaggaattgc taatattgac ctttctgggg
     8461 caggttccgc catcatgaat ggcttcttgg gtggtttgca gtctgcttgg gagggtgtca
     8521 agagtttcgt aggcggtatt gctggctgga ttgcggaaaa caagggtcct atctcatacg
     8581 accgtgtact tttgaaacct gctggtcttg ctatcatgca aggtttaaac accaacttgc
     8641 aagtgggatt caaggacgtc atgggaacgg tatccggtat ggctggagca attgctaagc
     8701 cttttgaaaa tcaatctttg gcctatgata tgacgtcaag tgcatcggtg gatgtacgtc
     8761 gaaacttgct gtcttctact ggtgatttgg ctggaaatga tggcggaaat agtttagcta
     8821 ctcgtctggc gaacatcgag cgattcctat ctgctttggt ggataaggag ttggcagttt
     8881 atctggacgg tgagaaattg gctcagaata gttacatgca tcaaggagcg attatggcaa
     8941 gggagggtat ttaatgaact atatgattat caatggctta gatacttcga ccttggcaga
     9001 ttgccatgtt cttgactttg gtaaagcaca aacttcagtc gagcggtctg aacaagtcga
     9061 ggtcttcggt gccaatggtc aactacatgt cagcgagggt gcgtatgatg gctacaacag
     9121 gacatttatc atcacgctac gacatttggc agatgctatg cgcttgattg aggtttttca
     9181 gtcggaaaat aatacagtag aattcggcta tctgagggat agcatttttt actgtaattt
     9241 ggtatctagt agctacgtgc cacttggtcc acatcgttgg aaggtggaaa ttacggtgtc
     9301 catgcatccg tttcggtatg ttaaaaatcc agctgacgtc gttttgacct catcaggttc
     9361 tgtgcagaat ccaggtacgg tctattctga acctatcatc atcatagagg gttcgggacg
     9421 ggtgaccttg accattggtc agcaattgat ggagttagaa ctagatactc gtgcgaccat
     9481 cgattgtcgg cataagcgac aaaatatcta tgacaagaat ggtgctgtaa agaacaccat
     9541 tcgcaaacgt ggtccattct ttgagatagc tgttggaaga agcggtattg caacaagtgg
     9601 aactgtctca aaaataacaa tcaaagggaa ttggaggtac aaggtttgat ttatctaaaa
     9661 gacggtaata tcccgcttaa tcttgcttac gatgatgaca tcgtgcagga ggccaatagc
     9721 acctaccaac tttcctttaa atttccgctg actgatggga agtggaatct gcttagacgg
     9781 gaagtttttc tgctggctga tgatctacac ggtgaacagg aattttttat ttttgaagta
     9841 aaaaaagcca aaggtcatgt gcaggtctat gctaagcagg tcgcaacatt attgaattac
     9901 tactctatca actctatttc ggttgacagg gtaccagggc agacggttat gactgctttg
     9961 gcaggtagcg ttaaacgacc atgtccattt acgtttttta gtgatatatt agaccgtcat
    10021 acgtttaatg agtccaatgt atctgtaatg gctgctttgg ccaaggataa acactctatt
    10081 gtcggtcagt ggggtggtga cttggtgcgt gacaagtacc aggttaaatt gttgaaaaat
    10141 ggcggcattg agaatgagtc gctatttata tataaaaaga atctcagtag ctatgaagag
    10201 tctgagaaca ttaacaactt aaagacacga ttgcatctca aaaagacgat caaaggacag
    10261 tctgagggtg aggcagaccg tgtaattgca gtgactgtgg atagtccgtt gattgggcaa
    10321 tatcgtcaaa tctacgaagc agatattgag gtcaatgacc aagatgtgac agatgtggct
    10381 agcttaactg cctacgggaa acgttatttc agttcaacac tttgcgattt ggttgaaaat
    10441 tctatcaatc tggatgtcaa aggcaagtct gatgtatctg ttaagatgtt cgacatggta
    10501 agtgtattcc acgagcgatt cgatgtggat ttgcgtttga aaatttctag ctaccatttc
    10561 ggaccgatgt ctaagcgatt gaagtcaatt ggttttggca aggtgtcgca gtcatttggc
    10621 tcgacagtag cgagcatggt cgctggcagt gttgataaag caactggaag attgtcagca
    10681 tcctttgaac agaaactgca gaaggaaatt gacaatgcaa accgccattt cgacgctgaa
    10741 ttcgacaagc gagttgaatc catcaatgac ggtatcgagc aagcccaagc tgaggctgag
    10801 cggtatgctg acgctattaa acaggaaatt gatactgaaa tcgcccaagt caaccaatcc
    10861 atgcaagcac aatccgagga acacgacaga caggtcgcag acatcttgtc caagactcaa
    10921 tctgtcgaat cacttgccaa ccaggcaaag tcggatgctg ctagtgcatt ggctagagct
    10981 aaccaggtaa agaccgaggc tatcgcagat gcgagagcgc aggtagcgac agtcagtcaa
    11041 gccttaaaca ctgccaagtc cgaactgcag acggctatcg ctagtgctga ccaaaaggcc
    11101 agagatagcc aagcgagtgc tacagctttg cgaaacgacc tcaacttgca agctagcaag
    11161 attttggagc aagcacgagc acagacagct ttgaccaatc gtgtgacgac cgttgaaacc
    11221 ttggcagatg gtacgaggtc aacagtcgca gaactctcta aaacggttaa taaagcgaca
    11281 ggggacatca ctagtgttac agctaggacc aagacagtag aagacactct gagccaaacg
    11341 aggacccaat acgaagctct aacacagacc gtcaatactc agacaggaca gattgagagt
    11401 atcaatcgaa agactgctga cttgcagagt ggcatagatg gcgtgacgga gcggtttgag
    11461 aatctgaggg ttggtgggac taacttattc aaaaatagtg atttcagtca aggggaaaaa
    11521 aattggcata aattgcctga aatccataat gaagctacag gtaagtatgt aagattgcca
    11581 gctcgtcttt ggacaatggc acaatttgtt gaagtcgagc aaggtgaaga ttatgttatt
    11641 agcctttatg caaagaaaat atcaga
//
LOCUS       DBNFEG010000052        11459 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.052, whole genome shotgun sequence.
ACCESSION   DBNFEG010000052 DBNFEG010000000
VERSION     DBNFEG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11459
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.052"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(251..1630)
                     /locus_tag="AC1PNG_000966"
     CDS             complement(251..1630)
                     /locus_tag="AC1PNG_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HIP6701477.1"
                     /translation="MAVIGDVVNISVKQFTRTKKKVLDENGEKVEIDAIVSDSPRNVL
                     LAMKSDNKLNDFLRHNEFTGEHEIVADVKLDAISMRKGQLPSAFESYLSVYLENHFKV
                     VFKTRALQDGIEAFFAEKTYNPVQEYMEHAYDSWDHKERLNQVFQTWLGAEDSIYVQK
                     IAEMFFVGAVSKVFNPWVKFDYTLDLVGGQGAGKTTFLQKIAVDWYTDSAKDFMDKDN
                     YEIMLKSLIVNDDEMVASRKTTFDELKAFVTKTDLTFRRSYGRRAEKFPKNFVIARTS
                     NKVEYLGDKTGERRFLPILVDAAKQFVKPFDMTDNDVLQLWGEAVAIYKKGFTLTFDE
                     DFEDELAVYKERFTYRDEAENQIYDYLDMLVPEEWESMSVVQQHQYTWAYFNNGVYRN
                     DAGFIYEGVKPQESVSSKQILKNVFDIDVAKGDKLARKIKLIMDNNQDWEYKVKKVNG
                     KAIRGYFRK"
     gene            complement(1614..2441)
                     /locus_tag="AC1PNG_000967"
     CDS             complement(1614..2441)
                     /locus_tag="AC1PNG_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HIP6701478.1"
                     /translation="MGMKKAALAYQKKGFSVIPISPSNKQPMIKFADKPAMTAQEIED
                     FWSQYPDSNIAVRTDKFFVIDIDLHGKHNGYESLANWEHLNLITPTLQAKTASGGKHI
                     FYFKHPDVSMTQMIGFLPGVDVKAHPNNYVLVAPSKTPKGEYAWDLEKSKEGGTMVTA
                     SRALVMAIKQEYLKKNNRSELDDIYYQIRNGAGKRNRTTEVFEMIVKGFGEEGSRNDT
                     AAKFAGTLLARSVDPNCVLELARIANNNSADPLSDRELSRTVDSMVQKHMRGGGSDW"
     gene            complement(2452..2850)
                     /gene="ssb"
                     /locus_tag="AC1PNG_000968"
     CDS             complement(2452..2850)
                     /gene="ssb"
                     /locus_tag="AC1PNG_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019778239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIP6701479.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNQAVATFTLAVNRNFKNQDGER
                     EADFINVVIWRQQAENLANWAKKGALIGVTGRIQTRSYDNQQGQRVYVTEVVAESFQL
                     LESRGQQSNSQDGSFGNSSPMDIQDEDLPF"
     gene            complement(2847..3143)
                     /locus_tag="AC1PNG_000969"
     CDS             complement(2847..3143)
                     /locus_tag="AC1PNG_000969"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701480.1"
                     /translation="MGFTELGRKRPIKIQGDTYDEILIYKYKGEVWGVCYKGREIDCV
                     YNYTKEDFFYSRIWGMTLKEIVKKIIQPLKRTEPGIFAMIDYELVRKALKVEKL"
     gene            complement(3143..3841)
                     /locus_tag="AC1PNG_000970"
     CDS             complement(3143..3841)
                     /locus_tag="AC1PNG_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HIP6701481.1"
                     /translation="MTVKESPIFATLCSIQTELVAPKGQYNSFGKYNYRSAEDILEAL
                     KPLLKKHDASLVLNDDIEQIGDRYYVRATVTLFACGSSISAQASAREEDTKKGMDGSQ
                     ITGTASSYARKYALNGLFAIDDNKDPDTDEYANQTGKQAQAQKAAQSKSTSNPKQNQN
                     ASGSVKYITGAQAKKLREDIKNIAEASGGPVNTVGVWFIGQLGVDKIESIPADRLKEA
                     QDLIAKTKKAKGME"
     gene            complement(3851..4117)
                     /locus_tag="AC1PNG_000971"
     CDS             complement(3851..4117)
                     /locus_tag="AC1PNG_000971"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701482.1"
                     /translation="MSEIKNNPHSLATKAGLVEEIRGALSLPYEYYLKQESEIKALAK
                     IHDCEIILVDAEYTLTYPNGEEVSKIIPKESNLFDDLMEAFKQL"
     gene            complement(4191..5363)
                     /locus_tag="AC1PNG_000972"
     CDS             complement(4191..5363)
                     /locus_tag="AC1PNG_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIP6701483.1"
                     /translation="MEIELRKWQAEAVKRSDRDCPGIFLEGLGGRGKTICAFEIAKHK
                     GAKKVLVLNNRLSILNGWIDTHNKLYSQDFRLEAMTDKRMQNIVANGEVLEFDVLIVD
                     EWQNMSSEANYKAYKKIRRDYSIGLSATPIRKKGQNFYPLEKTIFGQAEPNNKFDWQK
                     QHGKMKYDRFSYSKEKWEDFRDYESYVSGLPNFFRWEEIEEIEGAEENNGFEVIFEPM
                     WCLPANPEELDQLRRVNIVGKNGKYAMAKQSFGRKTFERYLTQTGFEVDFPKLKAVNA
                     DTPMLLQLDILLAKQSEMLIVSKSKQIVEVIYERHPEIGIWTGDKKEGHDRTNMVATS
                     QVLGVGVDGLQHRFKTIVVLDPSQPSDGDYDDYRQLLWRITGSRQQHDVRVVEFYF"
     gene            complement(5332..5796)
                     /locus_tag="AC1PNG_000973"
     CDS             complement(5332..5796)
                     /locus_tag="AC1PNG_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701484.1"
                     /translation="MRILAIDPSSNRIETSTTGIVLLDNAGLVSYWVVPFGARNFSRW
                     FREVGRDLEYDVVIVEEYQVRDNDYSRDNSVAETVEAVQACFPNVELVRNAGYVSDIP
                     DQLLRKLGLWTFDKSHHQDVRAAARLALFWAQRKDIEEVIQDIGNRITQMAS"
     gene            complement(5777..6022)
                     /locus_tag="AC1PNG_000974"
     CDS             complement(5777..6022)
                     /locus_tag="AC1PNG_000974"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701485.1"
                     /translation="MMGTLFEQRPRFEQLSTPGYRIALMANELTDLFGISFKEALKCI
                     ELDHKINDYDVKDGQLAGFGELLRDYLEVKQNENPSN"
     gene            complement(6019..6498)
                     /locus_tag="AC1PNG_000975"
     CDS             complement(6019..6498)
                     /locus_tag="AC1PNG_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HIP6701486.1"
                     /translation="MASIYELTGIFKQIAEMEGIDEETKLDTLESIDWTEQFEEKVEN
                     TVKVIKNKEADVDQLKEEIDRLTKRKKSIENDITRLKTGLQGAFEITGHDKIKTLLFT
                     VSLANNQPSVVVDEDLLPKKYFIQTLKPDKTAIKELLKAGKKVKGAVLQESRSLRIR"
     gene            complement(6498..6839)
                     /locus_tag="AC1PNG_000976"
     CDS             complement(6498..6839)
                     /locus_tag="AC1PNG_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701487.1"
                     /translation="MTQAERIREYYKQHPAASYDEVAEALKTSNSNVRANVSKDIKAG
                     RCVRLEDKSLDYSMHYIKNEALADLINWKNDTRREWVDMLTRAAEKETDNNTMRLLIK
                     EANKLMKEVTE"
     gene            complement(6844..6996)
                     /locus_tag="AC1PNG_000977"
     CDS             complement(6844..6996)
                     /locus_tag="AC1PNG_000977"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701488.1"
                     /translation="MQDNHTTIEISVEEYIQLRNKVNDLQTENRFLRTIVDSVAVVMK
                     NSGMVR"
     gene            complement(6986..7219)
                     /locus_tag="AC1PNG_000978"
     CDS             complement(6986..7219)
                     /locus_tag="AC1PNG_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701489.1"
                     /translation="MTLSLFAVPLLAVGIVEHRKAEKRRMHKEFEEIRRRDYLYGFKA
                     GMGYQSTCDTEKARNGLKRDAQQVDKEMARYAG"
     gene            complement(7275..7529)
                     /locus_tag="AC1PNG_000979"
     CDS             complement(7275..7529)
                     /locus_tag="AC1PNG_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701490.1"
                     /translation="MDKIFVELSDWIKSIIRDVVNEILLEKDNDDGFPEMMNRKDCIR
                     FLKVDGTVFDKYRKLPNFPKEQEGTKWKKRAIKIWLSEKD"
     gene            complement(7603..7929)
                     /locus_tag="AC1PNG_000980"
     CDS             complement(7603..7929)
                     /locus_tag="AC1PNG_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701491.1"
                     /translation="MVENLLGIPCGHNPVVLGSVVDGNTKIFGEIVTVFSGDIIFGNL
                     KKLDVYTEGEILKLSDLYDKYKDYISPNDSLYVVVTNGLSGTIYDCGHTRRGIWHIFA
                     KTAGYA"
     gene            complement(7998..8198)
                     /locus_tag="AC1PNG_000981"
     CDS             complement(7998..8198)
                     /locus_tag="AC1PNG_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6701492.1"
                     /translation="MAKPKITIAELRAKNNKMSQKELASKIGVAYQTIGAWEDDITVI
                     KGDNLLKLCEFFGVSSSDLLGR"
     gene            8215..8763
                     /locus_tag="AC1PNG_000982"
     CDS             8215..8763
                     /locus_tag="AC1PNG_000982"
                     /inference="COORDINATES: protein motif:HMM:NF024252.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6701493.1"
                     /translation="MILSHFLDLDYMIHDYKSSIKRFFDFFSSFFGICTKSLDIKSLF
                     AYNIPMNREVKTMADAKIKYPEVGQRIRELREMRGFEQLDIANQLGYKSQSTISKWES
                     GVNLPTGKKLILLAEMLDTSTDYILHGKISDTSTKDTTPTIDFKEMAAESMSYDGMPL
                     NDEDIDLIASILETRMKNRDKE"
     gene            8770..9153
                     /locus_tag="AC1PNG_000983"
     CDS             8770..9153
                     /locus_tag="AC1PNG_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIP6701494.1"
                     /translation="MMTPESVCAERGIDLVYFDGRDSDKKGIYNKRANMIAVDAYLDE
                     IQHKKVIYHELGHENHDPAQYDRRREQYELQADRNMIHYLVKEELSLMDDVKEFNYVH
                     FMEKYDLKTIVNETMVIEEFNNLVN"
     gene            9201..9932
                     /locus_tag="AC1PNG_000984"
     CDS             9201..9932
                     /locus_tag="AC1PNG_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701495.1"
                     /translation="MKKNNFVKIILIIFAIALVVAIAAMLMPVAFVGGLGAVWYFTKK
                     KPDSNKRNISIAFAVVGLLGSIFITPAMLNNQKNTSTTVATAPSISSTSSTETSTSTS
                     STTEASSSTEESTEPTTKNDGPEYTEESNAAFATAFMNALNQSLADNGVDINVRVQYY
                     GKNLIHVIVPQDFKYEPNTSIQKVADSVFQAKENYFTEWAIDNGYDLGYTYAPKLYIN
                     AEDDTTLAEEEGILERKMELKIDNN"
     gene            10129..11286
                     /locus_tag="AC1PNG_000985"
     CDS             10129..11286
                     /locus_tag="AC1PNG_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIP6701496.1"
                     /translation="MKYTKTKYPNIFTYETQKGLRYYVRRGYFVNGDKKEFSKSGLRS
                     LKDAQRILRDIEERIYHDEMDVNLELTLNEYWEIYSAKKEKTGQWNDTSIYTNAGIYR
                     TMIKEKWGNLPLKKINRNDYEEHLAEMLGQYRRNSVLTSNRLLNSILNDAVKNGNLRQ
                     NKLSGIYLGESELEPLNKELKIDDFQTWIKTAEELLSTTHFAFVYLTIFGLRRGEVCG
                     IRFMDVTFDSNNRAVLNIRDSRSNRTKDGAGRTKTESSVRYVVLNDRGSELLLQIMKT
                     AKQVKKKADVIVKQEKDYLSIRIKNNKYYLERPAFLNKIFKRVSEECGIYITPHIMRH
                     FFTTQSLIAGARPEDVMHFLGHASLQTTKQYTHIKEERAHNVTDLFDKKVL"
BASE COUNT         3249 a         2589 c         2051 g         3570 t
ORIGIN      
        1 ttattaactt aaatatttat ataaatgatg taactaacag tagagatgac ttataaaccc
       61 aatattacca agggtttagg gcggttacac cactttttta tttttttcta aaaaaacacc
      121 tttaaaccct tgatacgatt gagtttttca acggttacat cattggtgta accttgatgt
      181 aacttgatgt aaccttttga ctaaaagtta catcaaaaca cgttttcggt tacatcactt
      241 tttctgcata ttatttccgg aaatatcctc gaattgcttt gccatttacc ttcttaactt
      301 tgtactccca atcctggttg ttgtccatga tcagcttaat cttccgagct aatttatctc
      361 ctttggcaac atcgatgtca aaaacattct tcaggatctg cttcgatgac acactttctt
      421 gcggtttgac accttcgtaa ataaaaccgg catcattccg atacacacca ttgttgaaat
      481 aagcccatgt gtactgatgc tgttgaacta cggacatgct ttcccattct tccggaacca
      541 gcatatccaa ataatcatag atttggtttt ctgcctcatc gcgataagtg aagcgttctt
      601 tatagactgc cagctcatcc tcgaagtctt cgtcaaaggt caacgtgaag ccttttttgt
      661 aaattgcaac tgcctcaccc cacaactgca ggacatcatt gtccgtcatg tcgaatggct
      721 tgacgaattg tttagctgca tccaccaaga tcggtaggaa acgacgctca ccagttttat
      781 caccaagata ctcgaccttg ttgctggtcc gagcaatcac gaagtttttg ggaaactttt
      841 cagcacgacg accataggac ctacggaatg tcaaatcggt cttggtcaca aaagccttga
      901 gctcatcaaa cgtcgtcttc ctactagcca ccatctcgtc gtcattgaca atcagggatt
      961 ttagcatgat ctcgtagttg tccttgtcca tgaaatcctt agcagaatcc gtgtaccagt
     1021 caactgcgat cttttgcagg aatgttgtct taccagcacc ctgcccaccg accaggtcta
     1081 gagtgtagtc aaactttacc catggattga agaccttgga cacagccccg acgaaaaaca
     1141 tctccgctat tttctggaca tagatgctgt cctcagcacc tagccaggtc tggaagactt
     1201 gattcagacg ttccttgtgg tcccaactgt cataggcatg ttccatgtat tcctgaacag
     1261 gattataggt tttttctgca aagaatgcct caataccatc ttgcaaagcc ctggtcttga
     1321 acacaacctt gaaatgattt tccaggtaaa cactgagata agattcaaat gcggagggca
     1381 gctgaccttt cctcatgctg atagcatcca atttgacatc ggccacaatc tcatgttctc
     1441 cagtaaattc attgtgccgg aggaagtcat tgagcttgtt atcactcttc atggccaaca
     1501 gcacgtttcg aggactgtct gacacaatgg catcaatctc aaccttttct ccgttttcat
     1561 ccaagacctt tttcttcgtc ctagtaaact gcttgacaga gatatttaca acatcaccaa
     1621 tcactgccac ctcccctcat gtgtttctgt accatactat ctactgtccg actaagctct
     1681 ctgtcactca gcggatcagc agagttatta ttcgcaatcc gtgccaattc caaaacacag
     1741 tttggatcaa cacttctggc caataatgtt ccagcaaact tggctgccgt atcgtttcga
     1801 ctaccttcct caccaaagcc tttgacaatc atttcaaaga cctccgtggt ccgatttcgc
     1861 ttgcctgcac cattccgaat ttgataataa atgtcatcaa gttcgcttcg gttgttcttt
     1921 tttagatact cctgcttaat ggccatgact agtgctcgac tagcagtgac catggtcccg
     1981 ccttctttcg acttttccaa gtcccaggca tactcccctt tgggtgtttt tgatggagcg
     2041 accagaacat aattatttgg atgtgctttg acatcgacac caggtaggaa tccaatcatc
     2101 tgcgtcatgg aaacatctgg atgcttaaag taaaagatat gcttgccacc gctggcagtt
     2161 tttgcttgca aagtcggggt aatcaagttg agatgttccc agttggccag actttcatat
     2221 ccattatgtt ttccgtgcag gtcgatgtcg attacgaaga atttgtcagt ccggacagca
     2281 atattactat ccggatactg actccaaaaa tcttcaattt cctgtgcggt cattgctggt
     2341 ttatcagcaa attttatcat cggttgctta ttagaagggc ttataggaat gaccgaaaag
     2401 ccctttttct gataagccaa agccgctttc ttcataccca tctagcacct cctagaacgg
     2461 caaatcttcg tcttggatat ccataggact tgaatttcca aatgatccgt cttgagaatt
     2521 cgactgttgt ccacgacttt ccaagagttg gaaactttct gcaactacct cagtaacgta
     2581 gacacgttgc ccttgttgat tgtcatagct acgagtctga atgcgacctg taacaccaat
     2641 cagagcacct ttcttagccc aattcgccaa attctcagct tgttgacgcc aaatgactac
     2701 gttgataaag tccgcttcac gctccccatc ttgatttttg aagtttcgat tgacagccaa
     2761 ggtaaaagtc gcaaccgcct ggtttgacgg agtgtaacga agttctgcat cacgagtcat
     2821 gcgtcccact agtactacat tgttaatcat aatttttcca ccttcaaggc ctttctcacc
     2881 aattcgtaat caatcattgc aaaaattcct ggctcagttc tttttaatgg ctgaatgatt
     2941 ttcttaacaa tttctttcaa agtcataccc caaattcttg agtaaaagaa gtcttctttc
     3001 gtgtaattat aaacgcaatc aatctctctg cctttataac atacgcccca aacttcgcct
     3061 ttatatttgt aaatcaagat ttcgtcatag gtatcacctt gaattttgat tggacgtttt
     3121 cgcccaagtt ccgtaaatcc cattattcca ttcccttcgc tttcttagtt tttgcaatca
     3181 gatcctgtgc ttctttcaag cggtctgctg gaatactttc gattttatcc acgcccagct
     3241 ggccaatgaa ccacactcca acggtattaa ccggaccgcc tgaagcctcc gcaatattct
     3301 tgatatcttc acgtagcttc ttcgcctggg ctcccgtgat gtacttaaca ctgccagatg
     3361 cgttctggtt ctgtttcgga tttgatgtag acttactttg tgctgccttc tgagcttgtg
     3421 cttgctttcc agtttggttc gcatattcgt ccgtatcagg gtccttgtta tcatcaatgg
     3481 caaacaagcc gttaagagca tattttcgag cgtaactgga tgcagttccg gtgatctggc
     3541 tgccatccat tcctttcttt gtatcttctt cccttgctga cgcttgggca ctgattgaac
     3601 tgccgcaggc aaataatgtt actgtagcac gaacatagta gcggtcccca atctgttcga
     3661 tatcgtcatt taataccagg gatgcatcat gctttttgag taaaggcttt agtgcttcca
     3721 aaatatcctc tgcactacga tagttgtatt ttccgaaaga attgtactgt cctttcggtg
     3781 caaccagctc tgtctgaata ctgcacaggg ttgcaaatat tggcgattct tttactgtca
     3841 tgtattctcc ttaaagttgt ttgaatgctt ccatcaaatc atcaaataaa tttgattctt
     3901 tgggaataat tttagaaacc tcttctccat taggataagt aagtgtatac tcagcatcta
     3961 ccaagatgat ttcacaatca tgaattttcg caagagcttt aatttcggat tcttgtttca
     4021 aataatactc gtatggaagt gatagggcac cacggatttc ttctacaaga cctgctttcg
     4081 ttgcaagtga atggggatta tttttgattt ctgacataaa atacccattt ttttctcacg
     4141 caatacgatt aacttgtttg aaattttcat ttttttgctc cttttgtttt ttaaaaataa
     4201 aattctacga ccctaacgtc gtgttgttga cgactaccag taatccgcca aagaagttgc
     4261 cgatagtcat cgtaatctcc gtcactaggc tgggacggat ccagaactac aattgtttta
     4321 aaacggtgct ggagcccatc gaccccaact cctaatactt ggctagtggc gaccatattg
     4381 gtcctgtcat gcccttcttt cttatcaccg gtccaaatac cgatttcagg gtgccgttca
     4441 tagataacct caacgatctg cttggacttg ctgacaatta acatttccga ttgcttggcc
     4501 aagagaatgt ctagttgaag caacattggt gtatctgcat taaccgcttt aagcttcgga
     4561 aaatcaactt caaatcctgt ctgagtcaga tacctctcga aagtcttgcg gccaaatgac
     4621 tgcttggcca ttgcatactt accattcttt ccgacgatgt tcacccgtcg aagttgatca
     4681 agttcttctg gatttgctgg caggcaccac atcggctcaa agatgacttc aaatccattg
     4741 ttttcctcag ctccctcaat ttcctcaatc tcttcccaac ggaagaaatt tggcaaccct
     4801 gacacataac tttcatagtc acggaagtct tcccactttt ctttggaata actgaatcgg
     4861 tcatacttca tcttgccgtg ctgtttctgc caatcaaatt tattgtttgg ctccgcctgg
     4921 ccaaagattg ttttttctag cggatagaag ttttgacctt tctttcggat tggagttgca
     4981 gacaagccga ttgaatagtc acgtcggatt ttcttgtaag ctttatagtt agcttcactg
     5041 gacatattct gccactcgtc aacgattaag acatcaaact ccaggacctc accgtttgcc
     5101 acgatattct gcattcgctt gtctgtcatg gcttctagtc gaaaatcttg tgaatagagc
     5161 ttgttgtgag tgtcaatcca gccattcagg attgacagcc gattgttcag aaccaagacc
     5221 ttcttggctc ccttgtgctt tgcaatctca aaggcacaga tagttttccc acgaccgcca
     5281 agaccttcca gaaaaatacc aggacaatcg cggtcgcttc gtttcacggc ctcagcttgc
     5341 catttgcgta attcgatttc caatgtcttg aataacctcc tcgatgtctt ttctctgcgc
     5401 ccagaatagt gccaagcgag cagcagccct cacatcctga tgatgtgatt tgtcaaaagt
     5461 ccaaagacca agcttcctaa gtaactgatc aggaatatct gacacatagc cagcgttgcg
     5521 aacgagttcc acgtttggaa agcaggcttg aacagcttcc actgtttcag caactgagtt
     5581 atctcgagaa taatcattgt cacgtacctg gtattcttct acaatgacaa catcgtattc
     5641 caggtcacga ccaacttcac gaaaccaccg actgaaattt ctggcaccaa atgggaccac
     5701 ccaatagctg accagtccgg cattgtccaa cagcacaatt cctgtagtgg atgtttcaat
     5761 tctgttgctt gacggatcaa ttgctaggat tctcattttg tttgacctct aggtagtctc
     5821 tcaacaactc cccaaatcca gctaattgtc catcttttac gtcatagtca tttattttgt
     5881 gatcgagttc aatacatttt aaggcttcct taaagctaat cccgaacagg tcagttaatt
     5941 cattagccat taaagcaatc ctataccctg gtgttgacaa ttgttcaaat cttggccttt
     6001 gttcaaataa tgttcccatc atctaatcct caaacttcta ctctcttgca acactgcacc
     6061 cttgaccttc ttaccagctt tcagcaactc tttgatagct gttttgtctg gtttcaaagt
     6121 ttgaataaaa tatttcttag gcagcaggtc ttcatctaca accacagatg gttgattatt
     6181 ggccaagctg acagtaaaca gcaatgtttt gattttgtca tgccctgtga tttcaaatgc
     6241 accttgcagc cctgttttga gtcgtgtgat gtcattttca atagattttt ttcgtttggt
     6301 taagcggtcg atttcttctt tgagttgatc cacatctgct tctttattct tgatgacctt
     6361 gacggtgttt tcaacctttt cttcaaattg ttccgtccag tcaatagatt caagcgtatc
     6421 cagcttggtt tcttcatcaa taccttccat ttctgcaatc tgcttaaaga ttccagttag
     6481 ttcataaata ctagccatta ttccgttacc tctttcatta gtttattagc ttccttgata
     6541 agcaatcgca tggtattgtt atcagtttct ttttctgctg ctcttgtcag catatcaacc
     6601 cactccctcc tggtgtcatt cttccagttg attaggtctg ccaatgcttc attcttgatg
     6661 tagtgcatcg agtagtccaa tgacttatct tccaagcgga cacaccgtcc agccttgata
     6721 tctttggaca cgttcgcccg tacattacta tttgatgttt tgagtgcttc agccacttca
     6781 tcatagctag cagctggatg ttgtttataa tattccctaa tgcgttccgc ttgtgtcatc
     6841 ttcctacctc accattccac tatttttcat cacaaccgcc acagaatcaa caatcgttct
     6901 caaaaaacga ttttctgtct gcaagtcgtt taccttgttc cgaagttgaa tatattcttc
     6961 aacgctgatt tcaattgttg tatgattatc ctgcataccg tgccatctcc ttatctactt
     7021 gctgagcgtc tctctttagc ccgttacgag ctttttcggt gtcacaggta ctctgatacc
     7081 ccatgcccgc cttaaagccg tacaggtagt ctctgcgacg aatttcttca aactctttgt
     7141 gcatccgtct tttctctgcc ttccgatgtt ccacaatgcc tactgccagc aaaggcactg
     7201 cgaataaact taatgtcata attgcttctg tcataaattt tttcctcgtt tcagaccttg
     7261 tccagattgg tcttttagtc tttttcgcta agccaaattt taatagctct tttcttccat
     7321 tttgttccct cttgttcttt ggggaaattc ggtaattttc tatatttatc aaagacggtc
     7381 ccgtctacct ttagaaatct gatgcaatcc tttcggttca tcatttccgg gaaaccatcg
     7441 tcgttgtctt tttccagtaa aatctcgttt acaacgtctc ttattatcga ttttatccag
     7501 tcggacaact caacaaatat cttgtccata gtagcctcct gtgttataat ttgagtaagt
     7561 taattttgta agccactgtt cccgcagtgg tttttttgcg tgtcaggcat aacctgcggt
     7621 ttttgcaaat atatgccata tcccacgtct ggtatgtccg caatcgtaaa tagttccaga
     7681 taagccatta gtgacaacga catataaact atcgttcggc gagatatagt ctttatactt
     7741 atcgtacaaa tcagataact ttaaaatttc accttccgtg tatacatcca attttttcaa
     7801 atttccgaag ataatatcgc cactaaacac agtcacgatt tcaccgaaaa tcttggtatt
     7861 tccgtcgacc acactaccta aaacaactgg attgtgtccg catggaatcc ctagcaagtt
     7921 ttcaaccatc ccttctcctt tctagtttgt tagttgggta gtttctacat gataaaaaat
     7981 catgtatttt ttaaaaatta acgtccaagt aaatcagacg aacttactcc aaagaattcg
     8041 cacaatttta atagattatc gcctttaatg actgtaatgt catcttccca tgctccaatt
     8101 gtttgataag caacaccgat ttttgaagca agttctttct ggctcatctt attattcttt
     8161 gcacgaagtt ctgcaattgt gatttttggt tttgccattc agttctcctt tcacatgatt
     8221 ttaagtcatt tccttgacct tgactatatg atacatgatt ataaatcatc tatcaagcgt
     8281 ttttttgatt ttttttcatc tttttttggt atttgcacaa aatcacttga tataaaatca
     8341 ctttttgctt ataatatacc tatgaataga gaggtaaaaa ccatggctga tgcaaagata
     8401 aaataccctg aagtagggca aagaattaga gagctgagag aaatgagagg gttcgagcaa
     8461 ttagacatag ctaatcaact tggttacaaa tctcaaagca caatttcaaa atgggagagc
     8521 ggagtgaatt tgccaactgg caaaaaacta attttattgg ctgaaatgct agatacttcc
     8581 acagactaca ttcttcatgg aaaaattagt gacacttcca caaaagacac cactcccaca
     8641 atcgatttca aggaaatggc ggcagagtcc atgtcttatg acggcatgcc tctcaatgat
     8701 gaagatatcg acctcattgc ctcaatactc gaaactcgca tgaaaaacag agataaggaa
     8761 taattgccta tgatgacacc agaatcagtc tgcgcagagc gtggaattga tttggtctat
     8821 tttgacggta gagattcgga caagaaaggc atatacaaca agcgtgcgaa catgattgcg
     8881 gttgacgcct atttggacga aatccaacac aagaaagtca tctaccacga attgggccat
     8941 gaaaatcacg acccggcaca atacgaccga agacgtgaac agtatgaatt acaagcggat
     9001 aggaatatga tccattatct ggtcaaagaa gaattgtctt tgatggacga tgtcaaagaa
     9061 tttaattatg ttcactttat ggaaaaatac gacttaaaaa ccatagttaa tgagactatg
     9121 gtgattgagg agtttaataa cttagttaat taaaaatacg tgcacgccca ccgcacgaaa
     9181 aacgggcagg aggttgtttt atgaagaaga ataacttcgt aaaaataatt ttaattatct
     9241 ttgcgatagc gctggtagta gcaattgctg caatgttaat gcctgtagca tttgtaggtg
     9301 gtcttggagc agtttggtat tttactaaaa agaaacctga tagtaataaa cgcaacatct
     9361 ctatcgcttt tgctgtagtt ggattgttag gaagcatatt tattacacct gcaatgctaa
     9421 acaaccaaaa gaacacctca acaacagtag caactgcgcc ttcaatcagc tctacaagta
     9481 gcacagagac ttctacttca acaagtagta ccacagaagc ttcttcgtca actgaggaat
     9541 cgacagagcc gacaacaaaa aatgacggtc cagaatatac cgaagaatct aacgcagctt
     9601 ttgcaacagc atttatgaac gctctcaatc aatcccttgc agacaatgga gtagatatta
     9661 atgttagagt gcagtattac ggaaaaaatc tcattcatgt aattgtgcct caggatttta
     9721 aatatgaacc aaatactagt atccaaaaag tagcagatag cgtcttccaa gccaaagaaa
     9781 attactttac agaatgggct attgacaacg gctatgacct tggatacaca tacgctccta
     9841 aactatacat aaacgccgaa gacgatacta cgctggctga agaagaaggt atcctagaaa
     9901 gaaaaatgga attgaaaatc gacaacaact aatatttaaa cgtcgataaa taaaaaaatc
     9961 cccacacttc caaccgacca aagccgaagt gtagggtagt ccaataagca aagaccagct
    10021 tttcagcagg actttttgcg tactctaatt atatcacttt aaggaggtga tgccaatatc
    10081 ctttcaaaaa aaccttgtcc agattggtct tacagaaagg aaaagagaat gaaatataca
    10141 aaaacaaaat acccaaatat ttttacatac gaaacccaaa aaggattgcg ttactatgtc
    10201 cgcagaggat actttgttaa tggcgacaag aaagaattta gcaagagcgg actacgaagc
    10261 ttgaaggacg cccagagaat tctaagggat attgaagaaa gaatttatca tgatgaaatg
    10321 gatgtcaacc tagaactgac tttaaatgaa tactgggaaa tctactcagc aaagaaagaa
    10381 aaaacaggtc aatggaatga tacatccatc tacactaacg caggcattta tcgtaccatg
    10441 ataaaagaaa agtggggaaa cctcccgcta aaaaaaataa atcgcaacga ttatgaagaa
    10501 catctggcag aaatgcttgg ccagtaccgc agaaatagtg tgcttaccag taatcgactt
    10561 ttgaattcca ttttaaacga tgctgtcaaa aatggaaatc ttagacaaaa taaattgtct
    10621 ggtatttatc ttggggaatc agagttagaa cctcttaata aagagttaaa aatagatgat
    10681 tttcaaacat ggataaaaac cgcagaagaa ttactatcga ccacacattt tgcatttgtc
    10741 tacctgacta tttttggact tagacgcggg gaagtctgtg gtatcaggtt catggacgtt
    10801 actttcgatt cgaataatcg agcagtctta aatataagag actctcgttc caacaggaca
    10861 aaagatggtg ctggaagaac aaaaacagaa agttctgtgc gttacgttgt tttaaatgac
    10921 agaggcagtg agctgttgtt acagatcatg aaaacagcaa aacaagtcaa aaagaaagca
    10981 gacgtcattg ttaagcagga aaaggattac ctgagcatcc gaataaagaa taataaatac
    11041 tatttggaaa gacctgcttt tttaaacaaa atttttaaac gagtaagtga ggaatgtggt
    11101 atctatatca ctccacacat tatgcgtcac ttctttacca ctcaatccct tattgctgga
    11161 gccagaccag aagatgtcat gcacttcctg ggtcatgcaa gccttcaaac aaccaaacaa
    11221 tatactcaca tcaaagaaga acgagcacat aatgttaccg atctatttga caagaaagtt
    11281 ctataatttt ccccgacttc ttcccgacaa ttccccgaca actacaccaa gttatgacaa
    11341 tatcaacaat aaacattttt tcaaaaagct agtagtatca acgttttcaa gttgttacat
    11401 aaataccatg attggctggg tatagctttt tcttaacggc gagatagttt ttttatatt
//
LOCUS       DBNFEG010000053        11443 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.053, whole genome shotgun sequence.
ACCESSION   DBNFEG010000053 DBNFEG010000000
VERSION     DBNFEG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11443
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.053"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..428
                     /locus_tag="AC1PNG_000986"
     CDS             <1..428
                     /locus_tag="AC1PNG_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP6701497.1"
                     /translation="SQAVEPSSWSKGWFAQDLINLDEEKTAFENVSCTSLQDRMTILN
                     LLGMLGLSYDKAQQKVANLSGGERVRLSLAKVLLADANLLILDEPTNFLDMAAIEALE
                     TFLKEYEGSVLLVSHDQQFVETSVHRQWEIVQACLVKKS"
     gene            520..2595
                     /gene="pbp2b"
                     /locus_tag="AC1PNG_000987"
     CDS             520..2595
                     /gene="pbp2b"
                     /locus_tag="AC1PNG_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2B"
                     /protein_id="HIP6701498.1"
                     /translation="MKKRKNKPLEHDGNFISLRLNILFSIVIFLFLVLILRLADMQII
                     NHDFYSNKLSTASQKIISNGSIRGQIYDAQGKPLVENEIQQVVSFTRPNKMSAQEMKE
                     VANKLLQWVNVSDVTITQRDKADYYLADEEVYRQVVEALPKDKKYDSDGNYLSESTIY
                     SNAVDSIPAEALQYSEQDNKAIELFKQMNGATYFSTVNLVTDTLTSEQVAHIVANESQ
                     LPGISTTNNWQRTILPTSLSSIIGTVTSEQAGLPAEDAEYYLSKGYSSNDRVGTAYLE
                     KQYEEVLQGQREKKQINLDRNGNIESIETIQEGQQGNNIKLTIDLAFQDGVNAILRKY
                     FESELATGSALYSEGVYAVALNPSTGAVLAMSGYSHEKGSSTITEDALGTITSVFTPG
                     SIVKGATISSGWENGVISGNQVQLDEPIYFAGSAPITSWYGAYGSFNIDATEALEYSS
                     NIYMVKIALGLLGQTYSANMYLNDGDTLKNAMTKLRSTFAEYGLGASTGIDLPLESTG
                     FLPDKYTTANFITNAFGQFDNYTPMQMAQYAATIANNGTRISPHLVEGIYGNNAQGGL
                     GELIETVSGKEMNQVNISAEDMSLLRQGFYQVVNGSGRFNTGSAIGRGAAVTISAKTG
                     TAETYTTTASGDVVTAINTNVVAYAPSDNPQIAVAVVLPNLTNQSSTTTKTITQEIIN
                     LYQSLHPMN"
     gene            2604..3200
                     /gene="recR"
                     /locus_tag="AC1PNG_000988"
     CDS             2604..3200
                     /gene="recR"
                     /locus_tag="AC1PNG_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIP6701499.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMEDDVVNEFA
                     KNLLAAKRDLSYCSICGNLTDQDPCAICQDSTRDQSTILIVEDSRDVTALENIQEYHG
                     LYHVLHGLISPMNGIGPDDINLKTLLTRLMDNEVTEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            3305..4351
                     /locus_tag="AC1PNG_000989"
     CDS             3305..4351
                     /locus_tag="AC1PNG_000989"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIP6701500.1"
                     /translation="MSKETLILLYGGRSAEREVSVLSAESVMRAINYDKFFVKTYFIS
                     QTGDFIKTQEFSQTPSADEKLTTNATIVEEQKIRPSDIYEEKAVVFPVLHGPMGEDGS
                     IQGFLEVLRMPYVGTNILSSSVAMDKITTKRVLESAGISQVPYVAVIEGENIDDKIAE
                     IEGKLTYPVFVKPANMGSSVGISKAEDLAGLHKALDLAFKYDSRVLVEQGVNAREIEV
                     GLLGNADVKTTLPGEVVKDVAFYDYDAKYIDNKITMEIPAKIDEAIMDTMRDNAAKAF
                     RAIGGCGLSRCDFFLTEDGDIFLNELNTMPGFTQWSMYPLLWDNMGLAYPDLIEELVR
                     LAKEMFEKRESHLI"
     gene            4439..4819
                     /locus_tag="AC1PNG_000990"
     CDS             4439..4819
                     /locus_tag="AC1PNG_000990"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIP6701501.1"
                     /translation="MYQTIVKGEGLFQALSQGYGESIRTFGVTEKGETPVSLVNIGLA
                     ACITMCVQGYYASQEGNKTMPVQVESRLDNQRFEVLIGIGESVSAQKQKEILAYVEKK
                     CKVKALLREDLQFETSFYVLESVE"
     gene            4821..5897
                     /locus_tag="AC1PNG_000991"
     CDS             4821..5897
                     /locus_tag="AC1PNG_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6701502.1"
                     /translation="MFLAIEEMRQNKLRYGLILGLLILISYLVFFLTGLAYGLMQENR
                     TAVDKWQADYVLLNSESNRLITASKIDTSLLDQVDASDKALIRQQAGVAYVDENATSD
                     EKEKVNIFAVESDSFIVPNIVEGRLYEKTGEVLVDKTLSEVEGFGLGDQIYLSGSDEK
                     VTIVGYTDNAYFGVAPVVYMDFTAFAELMQADKQQAATSNLASAIVVRGDAASLPNEL
                     EKVAIADFIENLPGYKAQNMTFGFMIGFLIVISAIVIGIFIFVLTTQKSPIFGLMKIQ
                     GLSNGYISGSVLAQTFLLAGVGTVLGLAGTYLSSLVLPSAVPFENNWTFYIAIGLALV
                     VFALLGASFSVRSIFKVDPLRNLS"
     gene            5907..6575
                     /locus_tag="AC1PNG_000992"
     CDS             5907..6575
                     /locus_tag="AC1PNG_000992"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6701503.1"
                     /translation="MKTLIFENISKTFQDGSQTITALKPINFSIEEGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPTTGRVLINQSDYSDLPEKKRAKLRYKDIGFVLQASNLIPFLTV
                     EKQLTLVDKVNKKADPSKRNELLSELGVDHLKNKFPKDLSGGERQRVAIARALYNDPA
                     LILADEPTASLDSNRAFEVVELLAKEAKERNKSIIMVTHDQRMIEKCDKVYEMKDGVL
                     TQIR"
     gene            6944..8305
                     /locus_tag="AC1PNG_000993"
     CDS             6944..8305
                     /locus_tag="AC1PNG_000993"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIP6701504.1"
                     /translation="MKLTLHEVAQVLGAKNDISLYPDRAFNKVEFDSRLITEGDLFVP
                     LKGARDGHDFIPVAFENGCVVTLSEVSLDVPHILVEDCLAALQQLAAYYLEKTGVEVI
                     AVTGSNGKTTTKDMIHDVLATTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKLVLEMGQ
                     DHLGDIHLLSEIAKPSLSVITLFGEAHLEFFGSREEIAKGKLQIADGMEPGSKLLIPA
                     YPIADKLLPSHVDLIRFGEQADLQVTSLVESKDSLTFTVNFMDGDIFLPVTGKYNATN
                     AMVASYVGKTLGVSNEAIKSALASLNLTRNRTEWKKAANGADILSDVYNANPTAMRLI
                     LETFSSIPANEGGKKIAVLADMKELGSQSVELHNQMILSLSPDVLDTVIFYGQDIEGL
                     AQLASQMFPIGKVYFFRKNAEQDQFGDLVKQVKEVLGGKDQILFKGSNSMNLGKVVEA
                     VEK"
     gene            8396..9094
                     /locus_tag="AC1PNG_000994"
     CDS             8396..9094
                     /locus_tag="AC1PNG_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HIP6701505.1"
                     /translation="MSDFFSNQISEAPNLNVLEHLFLMIIWFFMLHLCFRCYQKRWFH
                     IVFWWLQFIQIVSLYTWYIVADSPLSESLPFYHCRLAMLFILWAKPGPLKRYFAYLGL
                     FGSLAAFIHPVFDPFAFPHLTFFTFVIGHYALTVNCMLYLLSDLEGEMLKGKEVVKYT
                     LIMNMLILGVALLTGGNYGFLRQAPLVNTNNLPLNFFLVTCLLCFSILSIQAMLIAYL
                     KKESKQMNSHILKK"
     gene            complement(9125..>11443)
                     /locus_tag="AC1PNG_000995"
     CDS             complement(9125..>11443)
                     /locus_tag="AC1PNG_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIP6701506.1"
                     /translation="GLSTKEVQERIQNQQTNHFKTKTSASNWEIFRRNVFTSFNALNF
                     AIFLALLAVQAWSNLFFFGVIVLNAVSGMLTEWRARRMIDKLNLMNKDFIRVVRDSKT
                     LSLAPEDIVLDDVLLLSAGEQVPSDAIVLEGIAEANEAMLTGESDLIVKNTGAELLSG
                     SYLVSGQIYAKVIHVGAENYANKLMLEAKTHKPIVSRILYNMDKIAKFTGKIIIPFGL
                     ALFLEAFFIKLLPLKDSVITSSTALLGMLPKGIALLTITSLLTAVIKLGMKNVLVQEM
                     YSVETLARVDVLCLDKTGTITQGKMTVENLLPLTNHYSLDTIQQILATYIQTSEDTNS
                     TAQAIRKEYGDLEHHYKASHIIPFSSDRKWGAMTIENIGRIFLGAPEMLLTQNPPSVS
                     EAQARGSRVLILALSQQSLPSSQNQLPENIEPLALLEIADPIREDAAETLAYLRSQEV
                     TLKIISGDNPVTVSHIAKEAGFADYDSYIDCSKVDDEELIARAETTAIFGRVSPHQKK
                     LLIQTLKAQGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLDSEFRDI
                     PEILFEGRRVVNNISHIAPIFLIKTIYSFLLGLICIASIALGKAEYLLVFPFIQVQMT
                     LIGQFVEGFPPFILTFERNIRPVEKHFLRKSLLLALPNALMVVLSVLIFHLMQVFGYL
                     NLHDMQTLSYYVLGSTGLLAVIRACLPLTKARLALIIYSVFGFFISSHFLHGLIEIHP
                     LNSHTLPIYSGLMLIIIPVFFWISYKQGAFKN"
BASE COUNT         3356 a         2135 c         2585 g         3367 t
ORIGIN      
        1 gctcccaagc agttgagcct tcaagctggt caaaaggttg gtttgcacag gatttgataa
       61 accttgatga agaaaagact gcttttgaaa atgttagttg tacctctctt caagatagga
      121 tgacgattct caatttatta ggtatgttag gactttctta cgataaggct cagcagaagg
      181 tagcaaatct gtctggtggt gagcgtgttc gtctctcctt agctaaggta ttgttggcgg
      241 atgcgaatct tttgatatta gatgaaccga caaattttct agatatggca gctattgagg
      301 ctttggaaac attcttgaaa gaatatgaag gaagtgtcct cttggtttcc catgatcaac
      361 agtttgtgga aacatctgtt cacaggcaat gggagatagt gcaagcttgt ctcgtgaaga
      421 aatcataaga aaatattttc tgaaaaggag gctctgtcta aaaaatattt tttgcctgtg
      481 gtataataag aaggatattt ctagaatgag gtctttgaag tgaaaaaaag aaagaacaag
      541 cctttggaac acgatggaaa ttttatttcc cttcgtttaa atattttatt ttctatcgtg
      601 attttccttt tcctagtttt gatattgcgt ctggcagata tgcagatcat caatcatgat
      661 ttttatagta ataaactgtc tactgccagt cagaaaatca tcagcaatgg ctctattcgc
      721 gggcagattt acgatgcgca gggaaaacct ttggttgaaa acgagattca gcaggtcgtc
      781 tccttcacac gtcccaacaa gatgtctgct caggaaatga aagaagtagc taataaactc
      841 ttgcagtggg tcaatgtttc agacgtaaca attactcaac gtgataaggc tgattattat
      901 ttggcagatg aagaggttta tcgtcaggta gtcgaggcct tgccaaaaga taaaaaatac
      961 gattccgatg gtaactactt gagcgaatca acaatttatt ctaatgcagt tgatagtatc
     1021 cctgccgaag cattacaata ttctgagcaa gacaataaag cgattgaact cttcaagcag
     1081 atgaatggag ctacttactt ctctactgtt aatttggtaa cggatactct gacttctgaa
     1141 caggttgctc atatcgtagc caatgaaagt caacttccag gcatttcaac gaccaataac
     1201 tggcaacgga cgattttacc aacttccctc agctccatta tcggtacagt aacgagtgaa
     1261 caggctggtt tgcctgcaga agatgcagaa tactatctat ccaagggcta ctcttcaaat
     1321 gaccgtgtgg gaactgccta cctcgagaaa caatatgaag aagtcttgca aggtcaacgt
     1381 gagaaaaaac aaataaacct agatagaaat ggaaatatcg aaagtattga gacaatccaa
     1441 gaaggacagc agggaaataa tattaaactg actattgatt tagcctttca agatggggtc
     1501 aatgctattt tgagaaaata ctttgaaagt gagctagcta ctggtagtgc gctttactcg
     1561 gaaggtgttt atgcagttgc attaaatcct tctacaggtg cagtacttgc tatgtcagga
     1621 tatagtcacg aaaaaggttc aagtactatt acggaggatg ctcttggtac cattacaagt
     1681 gtctttacac caggttccat cgtcaaagga gcgacaatca gctctggttg ggaaaatgga
     1741 gttatcagtg gtaatcaggt tcaattagat gaaccaatct attttgcagg ttcagcacca
     1801 attacatctt ggtatggggc ctatggtagt tttaatattg atgcgacaga ggctctggaa
     1861 tattcttcta acatctatat ggttaagatt gctttgggac tactcggtca gacctactca
     1921 gctaacatgt atttgaatga tggtgataca ttgaaaaacg ccatgactaa gcttcgttcg
     1981 acttttgcag aatatggtct tggtgcttct acaggtattg accttccact cgaatctaca
     2041 ggatttttac cagataagta tacgacagcc aactttatta ccaacgcttt tggacagttt
     2101 gataactata caccgatgca gatggcacag tatgcagcga ccattgcgaa taatgggaca
     2161 cggatttctc ctcacctcgt tgagggaatt tatgggaata atgcccaagg tggtttggga
     2221 gaattaattg agaccgtatc tggtaaggag atgaatcagg ttaatatctc cgctgaggat
     2281 atgtcacttc ttcgtcaagg tttctaccaa gttgtcaatg gtagtgggcg ctttaataca
     2341 ggtagtgcta tcggtcgtgg tgcggctgtg accatcagtg ccaagactgg tacagccgaa
     2401 acctatacaa caacagcttc aggagatgtg gttactgcga tcaacaccaa cgttgttgct
     2461 tatgcgccta gtgataatcc tcaaattgcg gtagcagttg tcctaccaaa tttgaccaat
     2521 caaagttcaa caactactaa aacaattact caagaaatta tcaatttata tcaatcactt
     2581 cacccaatga attaaggagg cttatgcttt accctacacc tattgccaag ttaattgata
     2641 gctattctaa attgccaggt atcggtataa aaacggctac ccgtttagct ttttatacta
     2701 ttggcatgga ggatgatgtc gttaatgagt ttgcaaaaaa tttattggca gccaagcgag
     2761 acctatctta ttgctctatt tgtggtaatt tgacagatca ggacccctgt gctatttgcc
     2821 aggactcaac gcgagaccaa tctactattt tgattgtaga ggatagtcga gatgttacag
     2881 ccttggaaaa tattcaagaa taccacggtc tttatcatgt cttgcatggc ttgatttctc
     2941 ccatgaatgg tattggacca gatgatatta atttgaaaac cctcctcact cgcctgatgg
     3001 acaatgaggt tacagaagtt attgtggcga ccaatgcaac agcagatgga gaagctacat
     3061 ctatgtatat ctcacgtgtc ctcaaacctg cgggaatcaa agtaacccga ctagctcgtg
     3121 gcttagcggt aggcagtgat attgaatatg cagatgaagt gaccctgttg agggctattg
     3181 aaaaccgcac agagctatag ctgttctgta gttagatacg ttcctaattt taagatagga
     3241 tagatatgat ttgttaaatt atgctatact aagagtaata gcgttgatac aggaaggaaa
     3301 atatatgtca aaagaaacct tgattttact atatggtggg cgttctgcag agcgtgaggt
     3361 atctgttctg tctgccgaaa gcgtcatgcg tgctatcaat tatgataaat tttttgtcaa
     3421 aacctatttc attagccaga caggggactt tattaagacg caagagtttt ctcaaacacc
     3481 gtctgccgat gaaaaactca cgacaaacgc gacgattgtt gaagagcaaa aaatccgtcc
     3541 gagcgatatt tatgaagaaa aggcggttgt tttccctgtt cttcatggcc caatgggaga
     3601 agacggatct attcaaggct tcttggaagt gcttcgtatg ccttatgttg gcactaatat
     3661 cttgtcttca agcgttgcta tggacaaaat cactaccaag cgcgtacttg aatcagcagg
     3721 aatctcccaa gtaccttatg tggcagttat cgagggtgaa aatattgacg ataagatcgc
     3781 agagattgaa ggaaaattga cttatccggt ctttgtaaaa ccagctaata tggggtcaag
     3841 tgtcggtatc tcaaaagctg aggatttagc tggcttgcat aaggcacttg atctggcctt
     3901 caagtatgac agccgtgtcc tcgttgagca gggtgttaat gcgcgtgaga ttgaggtcgg
     3961 tcttttggga aatgctgacg tgaaaaccac ccttcctggc gaagtagtca aagacgttgc
     4021 tttttatgac tacgatgcca aatacatcga caacaaaatc accatggaaa tcccagctaa
     4081 gattgacgag gctatcatgg acaccatgcg tgataatgct gccaaggcct tccgtgccat
     4141 cggtggttgt ggactttctc gctgtgattt cttcctgaca gaagacggcg acatcttcct
     4201 gaatgagctc aataccatgc caggctttac ccagtggtct atgtatccac tcctttggga
     4261 taatatgggc ctagcttatc cagacttgat tgaagagctt gttcgtttgg ccaaagaaat
     4321 gtttgaaaaa cgcgaaagtc acctcattta attattatca agattggaat cattccaatc
     4381 ttttttcttg caaaggtttg caaaaaggaa taaaatggaa caagttctgg aggtgtttat
     4441 gtatcaaacg atagtaaaag gggaaggtct gtttcaggct ctttctcaag gttatggcga
     4501 atcaattcgt acctttgggg taacggagaa gggtgagact ccggttagtc tagttaatat
     4561 tggcttagct gcctgtatca ccatgtgtgt gcagggctac tatgccagcc aagaaggcaa
     4621 taaaaccatg cctgttcagg tggagagtcg cttggacaat cagcgttttg aggtgttgat
     4681 tggaattggt gagtcggttt cagcgcagaa gcagaaagag attcttgcct atgtggaaaa
     4741 gaaatgtaaa gtcaaagctc ttttgaggga agacttgcag tttgagacaa gtttttatgt
     4801 cttagaaagt gtggagtaag atgtttttag caattgaaga aatgcgacaa aataagctgc
     4861 gttatggctt gattttagga ttattaatcc tgatttccta cctggtcttt ttcctgacgg
     4921 gtttggctta tggtctcatg caggaaaata gaacagcagt tgataaatgg caggcagatt
     4981 atgtcttgct taattcagaa agcaatcgct tgataacagc atccaaaatt gatacgagtc
     5041 tgcttgatca ggtggatgct agtgacaagg ccttgattcg ccaacaagct ggagttgcct
     5101 atgtggatga aaatgccacc tcagacgaaa aagaaaaggt aaacatcttt gctgttgaat
     5161 cagatagttt tatcgttcca aatattgtag aaggtcgttt gtatgaaaaa acgggagaag
     5221 tccttgtaga caaaaccttg tcagaagtag aaggttttgg gcttggagat caaatctatc
     5281 tgtcaggttc tgatgaaaaa gtgactattg taggttatac agacaatgct tattttggag
     5341 tggcgcctgt tgtatatatg gactttacgg catttgcaga attgatgcag gctgataaac
     5401 aacaagctgc aacgagtaat cttgctagtg ccattgtcgt tcgtggcgat gccgcttctc
     5461 taccaaatga gctggaaaaa gttgccattg cagactttat tgaaaatcta ccaggatata
     5521 aggcacaaaa tatgaccttt ggctttatga taggattctt gattgtcatt tctgccattg
     5581 ttattggtat ttttatcttt gttttgacaa cacagaaatc tccgattttt ggcttgatga
     5641 aaattcaagg tctttcaaat ggctatattt caggttctgt attggctcaa accttcctac
     5701 ttgctggtgt gggaacagtc cttggattgg ctgggactta tttgtcatcc cttgtcttgc
     5761 caagcgctgt tccatttgag aataactgga ccttctatat tgcaatcggc ctagcactag
     5821 ttgtatttgc cctattgggt gctagtttct ctgtacgttc tatctttaag gtagatccat
     5881 tacgaaatct atcttaggag gttgttatga aaacacttat ttttgaaaat attagtaaga
     5941 ccttccaaga tggcagccaa accattacgg ctcttaaacc aataaatttt agtattgaag
     6001 aaggcgaatt tgttgccatc attggaccat ctggttctgg gaagtcaaca tttttgacct
     6061 tggcaggtgg tttacaaaca cctacaacgg gtcgtgtttt aatcaatcaa tcggactatt
     6121 ctgatttacc tgagaaaaaa cgtgccaaat tgcgctataa ggacattggt tttgtcttgc
     6181 aggcttccaa tttgattcca tttctaacag tcgaaaaaca attaaccttg gttgataagg
     6241 tcaataagaa agctgatccg agcaagcgaa acgaactcct atctgaattg ggtgtggacc
     6301 atctcaaaaa caagttccct aaggatttgt caggcggaga acggcagcgt gtagctattg
     6361 ctcgtgcact ttataacgat ccagctttga ttctagctga tgaaccaaca gccagtctag
     6421 acagtaatcg cgcttttgaa gtggttgagt tattagctaa ggaagccaaa gaacggaaca
     6481 aatccatcat catggtaaca catgaccagc gcatgattga aaaatgcgac aaggtttacg
     6541 agatgaaaga tggtgtcctc actcaaattc gataaaaaat ggtataatgg gagcgggaaa
     6601 gaactcgact tgtcaaaaaa gagttcgtca acaagttttt atttctagtt gttgagctga
     6661 aacagtctat tcccagactg tttcactccc acccccgcac agctcaaact gtctgggaga
     6721 cagtttgagg ttggaaataa agcgaacact gatcgcatca gtaggtagga cagatttgga
     6781 gtgtaattac gaacagttga aatttgcttc gcaaatccca tctccaacct ttaatagtcc
     6841 actggactat taaagccaat caaccactgc gctgggatgt tgacgcaaat tttgagacgc
     6901 ggtgtggggg tggaaactag cctttagttt attggagaaa acaatgaaat taaccctaca
     6961 cgaagtcgct caggtcttgg gcgcaaaaaa tgatattagt ctttatcctg atagagcatt
     7021 caacaaggtc gagttcgaca gtcgcctgat tacagaaggg gatctttttg ttcccctcaa
     7081 gggagcgcgt gacggtcatg actttattcc tgtggctttt gaaaatggct gcgtagtaac
     7141 cctgtctgaa gttagccttg atgttcctca cattctagta gaggactgct tggctgcgct
     7201 tcaacaatta gcggcttact acctagagaa aacaggggta gaagtcattg ctgtgacagg
     7261 atcaaacggt aaaacgacca ccaaggacat gattcacgat gttctggcga cgacttacaa
     7321 gacttataag acacaaggca actacaacaa cgaaatcggt ctgccataca ccgtcctcca
     7381 catgcctgat gatacagaaa aactggtctt ggaaatgggc caagaccact tgggagatat
     7441 tcatcttcta tccgaaattg ccaaaccaag tctatcagtt attacccttt ttggtgaagc
     7501 gcatttagaa tttttcggct cacgagaaga gattgccaaa ggaaaattac agattgcaga
     7561 tggaatggag ccaggtagta aactcttgat cccagcatat ccgattgcgg ataagctctt
     7621 gccgagccat gtagacctca ttcgcttcgg tgagcaggct gatttgcagg tgactagtct
     7681 ggtcgagtca aaagatagcc tgaccttcac cgtcaatttt atggacggcg acattttcct
     7741 cccggtaacg ggcaagtaca atgccaccaa tgctatggta gcaagctatg tgggcaagac
     7801 cttgggtgtg tctaatgagg ccatcaagtc agccctagcc agtctaaact tgacccgcaa
     7861 ccgtaccgag tggaagaagg cggcaaatgg agcagatatt ctcagcgatg tctacaatgc
     7921 caatccaacg gctatgcgct tgattttaga aaccttctct agtattccag ccaatgaagg
     7981 cggtaagaaa attgctgtgt tagcagacat gaaagaactt ggcagtcagt cggttgagct
     8041 tcataatcag atgattttga gcttgtcgcc tgatgtgcta gacaccgtca ttttctacgg
     8101 tcaggatatt gaaggcctgg cccagttggc tagtcagatg ttcccaatag gaaaagtcta
     8161 tttctttagg aaaaatgcag agcaggacca atttggagac ctggtgaaac aagtcaagga
     8221 agtcctcggt gggaaagacc aaatcctctt taaaggctct aattccatga atcttgggaa
     8281 agtagtggaa gcagtagaaa agtagagagg cgagacctct cttttttggc aagtcttgtc
     8341 aaaacatcga aaagttgata tactgacttt tggggaaaga gaggaattta ataagatgag
     8401 tgattttttc agcaatcaga tttctgaagc accgaacctg aacgtattgg aacatctgtt
     8461 tctcatgatt atttggtttt tcatgctcca tctttgtttt cgttgttatc aaaaaagatg
     8521 gtttcatatt gttttttggt ggcttcaatt tatacagatt gtctcccttt atacttggta
     8581 tatcgtagct gattctcctc tttcagagag tcttcctttt tatcattgtc gcttggctat
     8641 gctttttatt ctttgggcga agcccggacc tttgaaacgt tattttgcct atctcggtct
     8701 ttttggatcg cttgcggctt ttattcatcc agtatttgac ccgtttgctt ttccacacct
     8761 taccttcttt acttttgtta ttggtcacta cgccttgacg gttaattgta tgttgtacct
     8821 gttatctgat ttagaagggg agatgttgaa gggaaaagag gtagtgaaat atacgcttat
     8881 tatgaatatg ctgattctag gagtagctct tttaactggc ggaaattatg gatttttgag
     8941 acaggcacct ttagtgaata ctaataatct tcctttgaat ttcttcttgg taacctgcct
     9001 actctgtttc tctattttga gtatacaagc tatgttaatt gcctatttga aaaaagaaag
     9061 caaacaaatg aattcgcata tattgaaaaa ataagacttg aaattagttt ttcaagtctt
     9121 atttttagtt tttaaatgcg ccttgtttgt agctaatcca gaaaaagaca gggataataa
     9181 tgagcattaa tccagaatag attggcaatg tgtgactgtt tagggggtgg atttcaatca
     9241 gaccatgtag gaaatgggag ctgatgaaga aaccgaaaac ggaataaata ataagagcca
     9301 gtcgagcttt tgtcagtggc aaacaggcac gaatgacagc cagtagacct gttgaaccaa
     9361 gaacataata agagagggtt tgcatgtcat gcaagtttaa gtatccaaat acttgcatta
     9421 aatggaaaat aaggacactg agaacaacca tgagagcatt tggtagggct aagagaagag
     9481 attttctgag aaaatgtttt tctacggggc ggatatttcg ttcaaaagtt aggataaaag
     9541 gaggaaaacc ttcgacaaat tgaccaatga gggtcatttg tacttgaata aatgggaaaa
     9601 ctaacaaata ttctgcttta cctagggcaa tactagcgat acagataagg ccaagtaaga
     9661 aagagtagat tgttttgata agaaagatgg gagcaatgtg ggagatgtta ttaacaacgc
     9721 gtcgtccctc aaagagaatc tcaggaatat cgcgaaattc agaatctagg aggactagat
     9781 tagcgatttg tctagtagcc ggatcgcctt ctgccataac aatggaacaa tcagcttcgc
     9841 gtagtgcaag aatatcattg accccatcac cggtcattgc agtagtatgt ccctgtgctt
     9901 tgagggtttg gatgagcaat tttttctgat gtggggatac gcgaccgaaa atggcggttg
     9961 tttcagctct ggcaattagt tcttcgtcgt caactttgga acaatcgata tagctatcgt
    10021 agtcagcaaa tccagcttcc ttagcaatgt gtgagaccgt cacaggattg tcaccagaga
    10081 ttatttttag tgtcacttct tgcgagcgaa gataggccag tgtttctgcg gcgtcttctc
    10141 ggatagggtc agcaatttct aatagagcta gaggttcaat attctcaggt agctgatttt
    10201 gagaagaagg aagggactgt tggctaagcg caagaatcag gacacgggaa ccacgagcct
    10261 gtgcctcaga aacagaagga ggattttgtg ttagtaacat ttctggtgcc cctagaaaaa
    10321 tgcgaccaat gttttcgatt gtcattgctc cccattttct atcgctagaa aatggaatga
    10381 tgtgactagc cttatagtgg tgctctaaat caccatattc ttttcgaatg gcctgagctg
    10441 ttgaattagt atcctcactt gtctggatat aggtagcgag tatctgttgg atagtgtcaa
    10501 gtgaataatg gttggttaag gggagcaggt tttcgacggt cattttcccc tgagtaatcg
    10561 ttccggtctt atctaaacaa agaacatcaa ctcgggcaag tgtttcgacg gaatacattt
    10621 cttgtacgag gacatttttc attcccagtt tgataacagc agttaagaga gaagtgatgg
    10681 tcagcagagc aattcccttt ggtaacatac ctagtagggc agtagagcta gtaataactg
    10741 agtcttttaa gggaagaagt ttgatgaaaa atgcttctag aaaaagagca agtccgaatg
    10801 gaatgataat ttttcctgta aatttagcaa ttttatccat attgtaaaga atacgagaga
    10861 cgataggttt gtgagttttc gcctcaagca tgagcttatt ggcatagttt tccgcgccaa
    10921 catggatgac ttttgcataa atctgtccgc taactagata gctaccagac aataattcag
    10981 ccccagtatt tttcacaatc aagtcgcttt caccggttaa catggcctcg tttgcctcgg
    11041 ctataccttc taaaacaata gcatcactag gaacttgttc gccagcagac agcaagagaa
    11101 catcatctaa aacaatatct tctggtgcaa gagagagtgt cttactgtca cgaacaactc
    11161 ggataaaatc cttgttcatc aggttgagtt tgtcaatcat tcgtcgtgct cgccattctg
    11221 tcagcattcc tgaaacagcg ttgaggacaa taactccgaa gaagaaaagg ttggaccatg
    11281 cctgaacagc tagtaaggct aagaaaatgg caaaattgag tgcgttaaaa gatgtaaaca
    11341 catttcgtct gaagatttcc caattactag cactggtctt tgtcttaaaa tgattggttt
    11401 gctggttttg aatacgttct tgtacttcct tggttgataa acc
//
LOCUS       DBNFEG010000054        11424 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.054, whole genome shotgun sequence.
ACCESSION   DBNFEG010000054 DBNFEG010000000
VERSION     DBNFEG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11424
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.054"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(75..713)
                     /locus_tag="AC1PNG_000996"
     CDS             complement(75..713)
                     /locus_tag="AC1PNG_000996"
                     /inference="COORDINATES: protein motif:HMM:NF012651.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIP6701507.1"
                     /translation="MNFEEMKSTLENMLNENYADFTKAIIAIEKDISDPDLLQQVYQA
                     YMDDDSISGLLHSNIDAIIETLESDKSQLSTDNVELVGNVTKDVEVKVFPGEDGKEFK
                     VANFSIARNDEQGNSHFTNVTAYNGRADFADQLKKGDFVKLTGQMKYEEYEGKLYKNL
                     VVKTIKILKTKEKQLEEAGKGEKPSTLGALKEYSQHTSSEPSKPKQDKGMEL"
     gene            complement(710..6267)
                     /locus_tag="AC1PNG_000997"
                     /pseudo
     CDS             complement(710..6267)
                     /locus_tag="AC1PNG_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989360.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(6753..7187)
                     /locus_tag="AC1PNG_000998"
     CDS             complement(6753..7187)
                     /locus_tag="AC1PNG_000998"
                     /inference="COORDINATES: protein motif:HMM:NF025561.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HIP6701508.1"
                     /translation="MNQALVIEKMNTTMTANEFSHCQTTEEIVEMIYQQTLHTLQTNP
                     QIQREIGTAPYAKERLEYLSKEESNVEMLLELYQTAQLIPHLMEVEKEAQTMGVILEQ
                     QLIQEWNLTEELKSKDMLKWTAQMNNLRSSIKEQVRYNLIYI"
     gene            complement(7978..8430)
                     /locus_tag="AC1PNG_000999"
     CDS             complement(7978..8430)
                     /locus_tag="AC1PNG_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701509.1"
                     /translation="MKLLHQKSIYESNYDEALQHDIEIDNIMSKLFSLPDYNSEFQLR
                     FEDDYHKEMNVPLDYESYLHNIFDFIAEQDIKNGVDVHLTDEGNLCFMAYGQSYTIRS
                     TGVSDVVRTSVTVISKDEAGNQVDFSQHFTIPVQEKEQMNKIKSEQVL"
     gene            complement(8427..9386)
                     /locus_tag="AC1PNG_001000"
     CDS             complement(8427..9386)
                     /locus_tag="AC1PNG_001000"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HIP6701510.1"
                     /translation="MKTLTIVELFGGIGALRKALLNQEIPHQLIDYVEIDRNCVKSYN
                     VLYNENFIPQSVKGYSLPNMQIDLLMHGSPCQDFSRSGLKRGGKRGSYTRSSLLFETI
                     LILKNAVQKPKWVVWENVVGVLDKKMKPTFEAYLTELEYLGYTSCYWILDSFDFGIPQ
                     KRKRIFVVSYLGRENQFDFSKLERTQAPAIEEFLECKVAEKYTVTQPSILKYIEKACQ
                     SIFTARVHIIQEYCYTISTKQTRVPNAGFIRLGTGKYRYLTERECFRLMGFTDKDFNS
                     LRTIFPEKISKKSSILYKQAGNSIVVPVLEAIVKQIYQKELNS"
     gene            complement(9383..10567)
                     /gene="dcm"
                     /locus_tag="AC1PNG_001001"
     CDS             complement(9383..10567)
                     /gene="dcm"
                     /locus_tag="AC1PNG_001001"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HIP6701511.1"
                     /translation="MYFFDLFAGIGGFRLGLEQAGHICVGFCEIDRFARNSYQAIFDT
                     EGEVEYHDIRKISNHAFRKLRGKVDLICGGFPCQAFSLAGRRLGFEDTRGTLFFEIAR
                     AAQQIQPQFLLLENVKGLLNHDQGRTFRIILSTLNELGYDAEWQVLDSKYFGVPQHRE
                     RVYIIGHSRRRSRQFLFPIGRKSKVSPLSGPKCMGNINSSGYGLNGKVYSSSAISPTL
                     TTNKGEGVKIAYPILTPERIEKRQKGRRVKQESGPMFTLTSQDRHGVVLEKRLPPSRG
                     VYVRSNTNKGFEAAFEGDSVNFAYPSSKVRRGRVGKQVAQTIVTGNSLGVVTNQLQIR
                     KLTPRECFRLQGFPDWAFNRASRVTSNSQLYKQAGNAVTVPVAFEIGKRLAAIEKEGE
                     SE"
     gene            complement(10644..10874)
                     /locus_tag="AC1PNG_001002"
     CDS             complement(10644..10874)
                     /locus_tag="AC1PNG_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701512.1"
                     /translation="MAKTMISPVELYSNELAQALLETSKYRLEASVAHQIARQYASQV
                     DFEDPILMHVGVNSIASTLIDKIKPEYFQTTS"
     gene            complement(10859..>11424)
                     /locus_tag="AC1PNG_001003"
     CDS             complement(10859..>11424)
                     /locus_tag="AC1PNG_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HIP6701513.1"
                     /translation="SSLLEGIKVDYNLGHELDFGAGFYITSDFQQAQKYINRLVEFLN
                     TNKAGAEFLGNMDEEGIILEFELTDFPSIFTTSNYKCHYFKSHNISDDVIDFAEFVFQ
                     NRLRSKERIHDFDFIYGVQTDDNPTGLLQKYKAGLVSKADVLNEFRKPFSFKQLSIHN
                     QEFCDIMKVTKIYLSKDGKELDQWQKQ"
BASE COUNT         3238 a         2502 c         1848 g         3836 t
ORIGIN      
        1 tcagaaaacg acaacaagtt tagagtaaat aaaaaaacaa gctagacctc cgaaaatggt
       61 ggtctagctt tttgtcacaa ctccatccct ttatcctgtt ttggtttact tggttctgaa
      121 cttgtatgtt gggagtattc cttcaaagct ccgagagttg agggcttttc tccttttcct
      181 gcctcctcta gttgtttttc ttttgtttta aggattttaa tagttttaac aacaagattt
      241 ttgtagagtt tcccttcata ctcttcatac ttcatctggc cagttagctt gacaaaatcc
      301 cctttcttga gttggtctgc aaagtcggca cgtccgttat aagctgtcac atttgtaaag
      361 tgactattcc cttgttcatc atttcgagca attgaaaaat tggcaacttt aaattccttt
      421 ccatcttctc cagggaagac tttaacctcg acatctttag ttacattgcc aaccaattct
      481 acattatctg ttgataattg acttttatcg gactccaaag tttcaattat tgcatcaata
      541 ttggaatgaa gtaaaccaga tatagaatca tcatccatgt atgcttgata gacttgctgc
      601 aacaagtctg gatctgaaat atctttctca atcgcaataa tggccttagt aaagtccgca
      661 taattctcat ttaacatatt ctctaaagta gatttcattt cttcaaaatt catattactt
      721 gctcctcttc tatttcttct tttgtttggc ttttgtagtc acgttcttca acttctgcct
      781 tataggcttg aatttgtgct aaagtagatg gccgtttttc tttctcgtcg acatcatagt
      841 ttgattcaaa agcatcacta tctttaaaat catggtcata tcggtctacg ataccatcat
      901 tatccaaatc cttgtcaagc aaatctctag tataaggtaa acctagcgtt tgacaagcct
      961 ccttttcatc aattgagagt aagtcctcat tggtggaatg ctccaaaaat agtttaaagt
     1021 gttgagcttg ctcctctggt gtacttcctt caaactggcc tgacattata tctgaatccg
     1081 caaaggataa tttccaggaa aaattggaca gttcaacact atactcaata tcatagtctc
     1141 caacctcgct ttttcccttt gctagataca ccacatctcc agtcgaaaag atgtctttaa
     1201 attgttccgt tctactttct tcagaaagtt ctgtatctcc atattctctg gctaagaagt
     1261 caacaattgc tgccttaaca tcttcaagta aaggattcgg attctctaac tcttccacat
     1321 cccccatatc caggatgcga ttgacctctg ctaaacgcaa agtcttttct tttagtaagt
     1381 ccgccttttc aaaaggtttc gttacctctt ctttcgcaac ctggagcttg tattggacct
     1441 ctgacattct tcatctatcc tatccaaaac attgtctata cgttgaatat tgccctcagg
     1501 gctatcaccg aaatgaccaa cgtaggataa attaccctca atcacaaaat ccattgcttg
     1561 agcaatttgg ttaaagaatg cggaaacttc aaatccacga tagtgaccaa ttgacattgt
     1621 acctgatgca ggaactagtg gtaaaatttt cattaattct tctgctgctg atttcttatc
     1681 tacaaagtgt tttcctaaaa tttccatacc attaaattga ccgtcacctt gtacttttga
     1741 aacctttctc tccatatctt gcttggcaaa tgcaatttgt tctgttagac gagcgatttc
     1801 gaacggatag tgttttgcta tcttatcttc taactgatat aaattggact tgtagtttga
     1861 ttccagcatc cgtaatttag atacttcaac atctaaatcc atcttctctt tgataagtgg
     1921 attaccagtc gcaagtgcct tgatttccgc ataatttaag gtattctcat ccacatcatc
     1981 agccacacgc acaggcgttt tcgaggtcat aatttgagag ataaacttct gtttattctc
     2041 aatggtctgc caaagatatg cgtcgaatgt attctccgtc acataacgat agatatagac
     2101 gtccttattc tcatttccct gacgaacgat ccgaccagac cgttgctcca aatctgctgg
     2161 tctccagggt acatccaagt cgtggagggc aatgagtttg ctttgcacgt ttgtacctgc
     2221 ccccatcttc gatgtggaac caaaaagaat acgaatatcg ccacttctca ccttggcaaa
     2281 caactcagct ttctgctttt cattattcgc atcatgaata aaggcgattt cattttctgg
     2341 aattccaaga ttaactaact tggttttcat atcatcgtag aggttaaaac ttccatcccc
     2401 cttcggtgta gacatgtcac aaaatacgag ctgagcggaa cgtcgctcac ttgattgttc
     2461 ccagattgcg aaaatgttct tggtacagac atttaccttg ctctctggat catctggtaa
     2521 aaaggaatta accaaacgct ggtctaaagc aatcttctta ccatcattcg taatccgcaa
     2581 catattatct tcctggggct cgactttcct atttctcacc ttatcagcac gctcggaaaa
     2641 gctttttaaa atttctttct gatgacctgt tggcttggta agtatggttt cataatgagc
     2701 ctgtggagtt ggtaaattca actgatctgc agtcttaata tctgccatct cttttacaag
     2761 agacatcagt tcaggaagat tgtaaaactt tgagaagcgt gtcttactac gataggagtc
     2821 tccttcagga gaaagttcta tggcagttac ggtttcgcca aaggtcgaag cccaggaatc
     2881 aaaatgatgt agcccttttc gttccagttc atcaaattgt agatatcgtt gcatggtgta
     2941 aagttcgctc atagaattcg acacaggtgt tcctgtcgca aaaacgacac cctttcctcc
     3001 tgttaattca tccaaatagc gacacttcat aaacatatcc gaagatttca tggcttcaga
     3061 ttgaccaatc ccagccacat ttctcatttt ggtgtaaaga taaagattct tatagttatg
     3121 ggcttcatca atgtagagtt tatcaacacc caattcctcg aaagtcacaa catcatcctt
     3181 acgaaaatcg tcatttagtt tttttagctt tgcctctagt ttcttcttcg tcccctcaag
     3241 ctgttttaca gtaaagtttt ggtctctctc atacttatat tcttcaatga actcgactat
     3301 atcctgaatt tgatgtttca gatgttgttc ctggtattct ctactcattg gaatcttctc
     3361 gaattgagtg tgaccaataa tgaccgcgtc gtattcgcca gtcgctatcc tacctacaaa
     3421 ccgttttcga ttagcaggtt caaaatcttt tttagtggct accataatat tggctgctgg
     3481 atacaactcc ataaattccc ttcccatctg ttctgtcaaa tggtttggaa caacaaacag
     3541 ggctttatta ctcatcccaa gtcgcttact ttccatagca cttgctacca tctcaaacgt
     3601 tttacctgct ccgactacat gagctaaaag ggtatttcct ccataaagcg ttcgtgctat
     3661 ggcattcttt tggtgtggac gtaggctaat atctgatgtc atcccctcaa gagagaggtg
     3721 ttggccatca tattctcggt ttcggattga gttaaatttt tcattgtaga gtttttcaag
     3781 cctatgccta cgactttggt ctttgaaaat ccagtctttg aaagattctt tgagaagttc
     3841 ctgcttttca ccggccaaca tggtttcttt cttgttcagg acttgttttt tatttcctgc
     3901 atcatcataa actgtatcgt agactcgtgt atctcgtaaa tttagtgaat cctcgattaa
     3961 ccgataggca ttgactcgac ttgtaccaaa agtcagattg gcaaggtcat ttcgactatc
     4021 ctgtgttttc ccatcaatct tccattccga tgtataggga gagaactgta ctttaattgc
     4081 ttttttcgca tagtagctag gttgaagcgt atcaaagata aactgctcaa tatctgaagt
     4141 tggaatccag gtagccccta gtcggactga aatttcactt gctgtcaatt ccttgggcat
     4201 aacttcttca agtctatcac gttgaaattt tagttcgtta atattctcaa gaacttcctt
     4261 atctggattg tcgattgatt gatacctatc aatgtaaaaa tccattatag aaatcttctc
     4321 acggatgtta ccgcttaaga attggtcagc aggaacataa gataatccca tttcttccat
     4381 cccttgctca gaaaatggat gaagcatact ctcctcatag gcaaaaggaa tatccaaata
     4441 gatttcccct tttaattctt ttatcaattc ctccctgggc ttgtcagtca actgtgtcat
     4501 atagtcgaag tcaactcttc ccttttcaga aattgaaaga atcaaggcct catgactagt
     4561 atcacaatgc tcgacaacct ttgcttttgc aattgtccgc ttaaagaaaa tatcgccctt
     4621 ccctacaaac ttacctttgt ctagcttctc aatagaagat actaagggat aatgactatc
     4681 ctcccgtaag ttcctgcgat taagtgtaga gttgataaaa tcatatttcg ctttaaaaga
     4741 atcatatgcc ttgttcagtt gctcttgact ttcttgaatc aaaacatcgc tggcattttc
     4801 tttttggagt tgaataactt ttttcaaact atccactacg tagatgtaag ctcttaatct
     4861 atttagttct ttaagtgata acttttgacg aatcatgaca gagtcttctc tgtaatatgg
     4921 aactccatca accaaagtat aagagaaatt cttaacatga tccattgctg gaagaatgtc
     4981 ctgtccatca tctaaattgt gaacttgttc ggcacgtggg atttctccct gaatggattg
     5041 aatagctgcg ctaagttgct cctgtaggtc actcccatct gaaagacagg ctaaactagg
     5101 accaaatcga cctgagactt ctgccatttg ccccaagacc atctctggat gctctacaaa
     5161 atacttattg taagtaaggc cattctcatc tctagctaaa tgtatccagt ccacatcctg
     5221 ctctctcaca acatcccttt tttgtaagac tagaatatca cttgtaacct cagtacctgc
     5281 taacccctta aaggtatcat ttggcaatcg aatagcaccc aacaagtcac atcgattggc
     5341 caaatattgt cgaacacgac tgtcttgttt atccattgtt ccagaagaag ttacaaatgc
     5401 aataatacct ccactacgaa ttttgtcgat tgacttggca aaaaagtagt catggataag
     5461 aaagttttct cggtcataat ctcgatcagt tagtttgaaa tctccaaacg gcacattccc
     5521 aaccgctaca tcaaagaaat tatttgaaaa tcttgtttct tcaaatccct ttatctgcac
     5581 atttgatttt tgataaagtt cctgggctat cctaccactc aacttatcca gctcaactcc
     5641 atacacctta gaacgattca tatcttttgg tagacgaccg ataaaattcc ctacaccaca
     5701 agagggttcc agaatattgc ctttctcaaa accaagattc tgcaagactt tataaataga
     5761 atcaatcacg agtggtggag tataaaaagc agtcaaagtt gattccttgg ctgcttgata
     5821 ttcttctggt gataataatt cttgtaattt acttcgagca tcagcccatt gacctgtctt
     5881 ctcctcattg aagacatctg caagacctcc ccaaccaaca tataatgaaa gagtttcttg
     5941 ttcttgtcgg ttggctctcc tgccttctgt ctcaatattc tttatgattt gaattgcggc
     6001 aagattattt tctaagcgtt gtgacggagg taatgaacct ggtagatctt caagtacaaa
     6061 attattcaat ggtctttctt cagtcttaac tgtggtggac tctgcaattc tgtatttttc
     6121 agaattctcg tgttctactt ctgcagtttc atcagatgtt tcctctccta aggcatcaaa
     6181 atcaaagagg tctaattgct catgctcctt cattttttca agcaatagac tgttttctaa
     6241 gtttcgtgca tactcatcaa tttcgtatct gaatcgttct tctaattcca gtacatagtt
     6301 aatctgacca accgtatcaa tgcgagcaac catttcatct tccatgtaga cgatgatttg
     6361 tccattttct tgtttatcaa attcaaataa tggtgaggac tcaaagtcag aaaaaatatg
     6421 gtcattaaaa cgataaagtt tattttcatc atcagaaaga taggtttctt cttccaatag
     6481 agcatctatt tcacccgcaa cttgtcgcca actgaatgat tccctatgtc catcggattt
     6541 acgtgttaaa tgtaatccct ttgaatcata ttgataggaa attggaaaac ttggaccact
     6601 tgctccaccc cagccgtatt ccttttgtaa aaactgggct tgaaatttta tatcttcttg
     6661 actagcaaaa aagtgagcaa ctcttagtcg cccaccttgt gttcctgttc ctttacgaag
     6721 aacatcatgc aattcttctt tagttatttt tactaaatgt aaatcagatt gtaccttacc
     6781 tgttccttga tcgaggagcg taggttgttc atctgagctg tccacttcag catgtccttg
     6841 gacttcaatt cctcggtcaa attccactct tgtatcaatt gctgttcgag tattaccccc
     6901 atcgtctgag cctctttctc tacttccatc aagtgaggaa tcaactgggc tgtctgatac
     6961 aattctagca acatttccac attgctctcc tccttgctca gatattccaa tcgttccttg
     7021 gcgtagggag ctgtcccaat ctctctctga atctgtggat tggtttgaag tgtatgaaga
     7081 gtttgttgat aaatcatctc tactatctcc tctgttgtct gacaatgact gaactcgttg
     7141 gctgtcatgg tcgtattcat cttctcgata actaatgctt gattcatttg gttgacctcc
     7201 tctatcttct acaccatcat tataccgctc aataatctct tttgtctgct cttttaatgc
     7261 aattttctca atcttttttt ggtgaataat acgaaaaatt ttttgggaca ttcttgataa
     7321 gtaatcacca aaaagcagaa actgtctagg actaaattga cctaacccat tctcaagtaa
     7381 atcattgttt aggaccatct caaccccaag tctaccagca aatactgctt gaaaactttg
     7441 ttcaataaaa cgaagtaaat agttttcact ttccacatct aaattcattc ctgctagaac
     7501 ttttggacca atgcctttgg tagctaatcc tgccaaataa cgaatcgctg cctcttcatt
     7561 ttccggtcta agtgacaatc tctcatcaat aatatctgta aggatggata aatcttttcc
     7621 tgattcatat ctccattgtt caattggtcg tctatcctta ttcactagga ctgtctggct
     7681 aatatcaaag acatacccta cgctagtcgt tgattgatta gataataata tcggtacccc
     7741 tttttgacca cgtctaacaa tatagtcaac ggacttccaa gattgaaaat ctgagcataa
     7801 gctagctgtc ggtttctgat gaataatact caattggttt tgaaagtcaa aaccaacgac
     7861 ataacctagc cgccctagca tcctttgata agcatctaca tctgaaagat aatgagtgga
     7921 taagtctccg acatagttgg catattcttc aactcgcatg acttctcctt tctttcgcta
     7981 taaaacctgt tctgacttga ttttgttcat ctgctctttt tcctgtactg gaatagtaaa
     8041 gtgctgacta aagtccacct ggtttcccgc ttcatcttta gagatgactg tgacagaagt
     8101 tcgtaccaca tctgacactc cagtagagcg aatcgtgtaa gattgaccgt aagccataaa
     8161 acatagattt ccttcatcag tcaaatgaac gtctacccca ttcttaatat cttgttctgc
     8221 tataaaatcg aagatgttat ggagatatga ttcataatca agtggaacgt tcatttcctt
     8281 atgataatca tcctcaaacc tcagttgaaa ctcagaatta taatctggaa gtgaaaacag
     8341 ttttgacata atattatcaa tttctatgtc atgttgtaag gcttcatcat aattactttc
     8401 gtaaattgat ttttgatgta atagtttcat gagtttaact ccttctgata aatttgtttt
     8461 acaattgctt caagtaccgg tactacaata gaattgcctg cttgcttgta caagatactg
     8521 gatttcttac taattttctc tggaaaaatt gttcgtaatg aattaaaatc cttgtcagta
     8581 aatcccatca atcggaaaca ttctctttct gttaagtaac gatattttcc agtaccgagc
     8641 ctaataaatc cagcattagg tactcgtgtt tgcttagtcg aaatcgtata acaatattct
     8701 tgaataatgt gaactcgtgc tgtgaaaatt gactgacatg ccttttctat gtacttaaga
     8761 atggacggtt gagtgacagt atatttttca gctaccttgc attctaaaaa ttcttcaata
     8821 gctggtgctt gtgttcgctc tagtttagaa aaatcaaatt gattttccct acctagataa
     8881 ctgaccacaa aaatccgttt ccttttttga ggtatcccaa agtcaaagct atccagtatc
     8941 cagtaacacg atgtatagcc taaatattcc aattccgtta gataggcttc aaatgttggt
     9001 ttcattttct tatctaacac gccaacaaca ttctcccaaa caacccattt aggtttttga
     9061 accgcatttt ttaatatcaa gattgtttca aacaagagag aacttctagt ataggagcct
     9121 ctcttcccac ctcttttaag tcctgagcgt gaaaaatctt gacaaggact gccatgcatt
     9181 aacaagtcaa tctgcatatt gggtagagaa taccctttaa cagactgtgg aataaagttc
     9241 tcattataga gtacgttgta gctctttaca caattcctat ctatttctac atagtcaatc
     9301 aattgatgag gaatttcctg attaagaagt gcttttcgga gggctcctat tcctccgaat
     9361 aattcaacaa ttgtcagagt cttcattcac tctccccctc cttctcaatt gctgctaaac
     9421 gtttgccaat ttcaaatgca acaggtacag tcactgcatt tcctgcttgc ttatacaatt
     9481 gtgaatttga agttactcta cttgctcgat taaatgccca atcaggaaat ccctgtaaac
     9541 gaaaacactc ccttggagtt agcttacgaa tctgtaattg attcgttaca accccaagag
     9601 agtttccagt aactattgtt tgagcaacct gcttaccaac tcgacctctt ctaactttag
     9661 atgacggata agcgaaattg acagaatctc cttcaaatgc agcttcaaat cccttattgg
     9721 tatttgagcg tacataaact cccctactcg gaggtaaacg tttttctaaa acaacaccgt
     9781 gcctgtcctg agaagtaagt gtaaacatgg gtcctgattc ttgttttacc ctcctacctt
     9841 tctgtctttt ctcaattctt tcaggagtta gaattggata agcaatctta acgccctcgc
     9901 ctttattcgt tgtaagagtg ggagaaatag cactagaaga atacaccttt ccattcaatc
     9961 catatcccga tgaatttatg tttcccatgc attttggacc tgatagagga gagactttac
    10021 tttttcgtcc gataggaaaa aggaactgtc tacttcgtct tctagaatgt ccaataatat
    10081 agactcgttc cctgtgttga gggacaccaa aatatttgct gtcaagtacc tgccattcag
    10141 catcataccc caattcatta agcgtggaaa ggataattct gaacgtcctc ccttggtcat
    10201 gattgagtaa gcctttgacg ttttcaagca ataaaaactg tggttggatt tgttgggccg
    10261 ctcgagcaat ctcgaagaac aaagttcctc tagtatcctc aaatcccaat cttcttcctg
    10321 caagcgaaaa tgcctggcaa gggaatccac cacagatgag atcaactttt cctcggagct
    10381 ttcggaaagc atgattggat atttttcgta tgtcatgata ttcgacttct ccttctgtat
    10441 caaaaatagc ctgataggag ttacgagcga atctgtctat ctcacaaaag ccaacacaaa
    10501 tatgtccagc ttgttcaaga cctaatcgaa aacctcctat cccagcaaat aaatcaaaaa
    10561 aatacattta tcctcctttt tgacaaagaa aaaagcgact agatttctct aatcgctaaa
    10621 atcatcattc aagtttaaaa actttatgaa gttgtttgaa aatattcagg tttaattttg
    10681 tcaatcaagg tgcttgcaat ggagttaact ccaacgtgca tcaaaattgg atcttcaaaa
    10741 tctacctgac ttgcatattg tcttgctatc tgatgcgcaa cactagcctc aagtctatat
    10801 tttgatgttt ctaacaaagc ctgtgctaac tcattgctat acaattcaac aggactaatc
    10861 attgtttttg ccattggtct aactcctttc catcttttga taaataaatc tttgttacct
    10921 tcattatatc acaaaactct tgattgtgaa tggataactg tttaaaagag aacggcttac
    10981 gaaattcgtt caatacatca gcttttgaaa ccaatccagc cttatatttt tggagtaaac
    11041 cagttggatt gtcatccgtc tgaactccat aaataaaatc gaaatcatga atgcgttcct
    11101 ttgagcgaag ccgattctga aaaacgaact cagcgaaatc tatcacatca tcagaaatat
    11161 tgtggctttt gaaataatga cacttataat ttgatgttgt aaaaattgaa ggaaaatctg
    11221 tcaactcaaa ctctagaata atcccttcct catccatatt ccccaaaaat tcagcaccag
    11281 ctttattggt atttaaaaat tctaccaaac ggtttatata cttctgtgcc tgttgaaaat
    11341 ctgatgtaat ataaaaacca gcaccaaaat ccagttcatg tcctaaattg tagtctactt
    11401 ttatcccttc taataaggaa gaga
//
LOCUS       DBNFEG010000055        11404 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.055, whole genome shotgun sequence.
ACCESSION   DBNFEG010000055 DBNFEG010000000
VERSION     DBNFEG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11404
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.055"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(3..911)
                     /locus_tag="AC1PNG_001004"
     CDS             complement(3..911)
                     /locus_tag="AC1PNG_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIP6701514.1"
                     /translation="MAEKRFGILITLIAGIAWGLSGVSGQYLMSRGVSVDMLTSLRLL
                     VSGFFLVGLAYATAKEQLLIVLKNRKALLGIFVFAMLGLVLNQMAYLQAIYHTNAGTA
                     TVLQYLCPILVLAYTCLKNREKPSGIELISIILAVAGTFLIATHGKLDELAVTPIGLF
                     RGIFSAFTYALYIILPGKLIRQYGSITVIGLGMLMGGFVVTLGLQTWQHRLPIDGGSF
                     LGLLGIVGVGTIFAYTAFLKGVSMVGPVNGSLLASIEPIASVFFAVWLVNEQFYAIDF
                     VGMLFILLAVLLISLKDILVAQKSIG"
     gene            complement(986..2467)
                     /gene="galT"
                     /locus_tag="AC1PNG_001005"
     CDS             complement(986..2467)
                     /gene="galT"
                     /locus_tag="AC1PNG_001005"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HIP6701515.1"
                     /translation="MMAGLVDRFVEQVIANSDFEEMDALYLHNRVLALVGDQVLTVQT
                     DLEDLIELKDELLAHGVRTGFVGELLEEQDMVGACLMDLMTPSPSQVNRDFWQTYQDS
                     PEQAIGDFYELSKRNDYIKMAAIAKNIYYQVSTEYGDLEITINLSKPEKDPKSIAAAT
                     KAEASNYPKCLLCMENEGYQGRINHPARANHRIIRLELGQEKWGFQYSPYAYYNEHAI
                     FLNQEHVPMVISPRTFEQLLDLLDLLPGYFVGSNSDLPISGGSILTHNHYQGGRHSFA
                     MEKAPIERQLVFDGFESVSAGIVKWPMSVIRLSSADKPSLLGLATKILEKWRSYSDDN
                     VQIKAETDGTPHHTITPIARKRGDLYELDLVLRDNQTSEEFPDGIYHPHPDVQHIKKE
                     NIGLIEVMGLAILPPRLKAELAEVEKFLLGQDSQVADYHQPWAESLKTAHPDVTEETV
                     EQVVRESVGQIFARVLEDAGVYKRTPEGQAAFLRFVEFVGLAT"
     gene            complement(2477..3649)
                     /locus_tag="AC1PNG_001006"
     CDS             complement(2477..3649)
                     /locus_tag="AC1PNG_001006"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIP6701516.1"
                     /translation="MNTEQLKQAFLDVFGQEADATFFSPGRINLIGEHTDYNGGHVFP
                     AAITLGTYGAARKRDDQLLRFYSANFEELGIIEVDLNNLVFDKADNWTNYAKGVLKFL
                     QEAGHAIDTGMEVFVYGNIPNGSGLSSSASLELLIGIIAEELYGLELTRLDLVKIGKQ
                     TENHFIGVNSGIMDQFAIGMGADNRAIYLDTNTLEYDLVPLDLGDHVIVIMNTNKRRE
                     LADSKYNERRAECEKAVEELNAVLNIQTLGELDEWTFDQYSYLIKDENRIKRARHAVL
                     ENQRTLQARKALEEGDLATFGRLVNASHVSLEHDYEVTGLELDTLAHTAWEQEGVLGA
                     RMTGAGFGGCGIAIVHKDKVEAFTENVGKTYTEVVGYEPSFYVAEIAGGSRVLSRK"
     gene            3782..4780
                     /locus_tag="AC1PNG_001007"
     CDS             3782..4780
                     /locus_tag="AC1PNG_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIP6701517.1"
                     /translation="MVTIKDIAKQAHLSSATVSRVLKGDLTLSVSQETRDRIFNLAQD
                     LGYTKHLKKQAPQQKGTIGIVQWYTESEELADLYYYSIRASIEQTASLLGYQIIRSFN
                     DLTHPLLQEVDGIIAVGKFSSGQIAELTNLSSKLIFVDSDTLTEGFSCITTDFEHSVQ
                     TVIDHFRSQGLTDIGLLVGQEETTDGYQLPTDPRLTAFRDYLDTLGMLQEDYIYQGKF
                     STQSGYELMSQAIEDLGDQLPPAFFMANDTLAVGALRALQERQIAVPGRVQLITFNDT
                     AITRQVYPALSSISVFTEEMGQEAMQLLDRVIASPQPHHPRKIKLGTQLVIRESSY"
     gene            complement(5083..5238)
                     /locus_tag="AC1PNG_001008"
     CDS             complement(5083..5238)
                     /locus_tag="AC1PNG_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HIP6701518.1"
                     /translation="MPMGNSSKSDGKEEHLLTAFEVVQTILGFGSFTIALIGLCYKIF
                     KDNDKKK"
     gene            complement(5556..6995)
                     /gene="gatB"
                     /locus_tag="AC1PNG_001009"
     CDS             complement(5556..6995)
                     /gene="gatB"
                     /locus_tag="AC1PNG_001009"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIP6701519.1"
                     /translation="MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKDMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIELEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FNYVRKGLQHEVERQAKILRSGGQIQQETRRYDESTGETILMRVKEGSADYRYFPEPD
                     LPLYEIDDSWIDEVRAELPVFPKARRAHYVENLGLTTYDAGQLTSTKALSDFFEAAVA
                     AGGDAKQVSNWLQGEVAQFLNAEGKTIEQIALTPENLVEMIALIADGTISSKIAKKVF
                     VHLAKEGGSAKAYVEKAGLVQISDPAVLIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVAQQLLAQELAKLLD"
     gene            complement(6995..8461)
                     /gene="gatA"
                     /locus_tag="AC1PNG_001010"
     CDS             complement(6995..8461)
                     /gene="gatA"
                     /locus_tag="AC1PNG_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIP6701520.1"
                     /translation="MTFNNKTIDELHDLLVKKEISALELTKATLEDIKSREGAVDAFL
                     TITEDAALAQAAALDEKGIDADNVMAGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATSVSQAYAKDMIVVGKTNMDEFAMGGSNENSAFKPTKNAWDQTKVPGGSSGGSAAAV
                     AAGQVRLSLGSDTGGSIRQPAAFNGIVGMKPTYGTVSRFGLIAFGSSLDQIGPFSQTV
                     KENAQLLNVISGHDVKDATSTINEIADFTSKIGQDIKGMKIALPKEYMGEGIDPQVKE
                     TILKAAKHLESLGAIIEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGFRAEDA
                     TNLEEIYVKTRSQGFGEEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGLSIPAGFVEGLPVG
                     LQLIGPKYSEETIYQVAAAFEATTDYHKQQPVIFGGAN"
     gene            complement(8461..8763)
                     /gene="gatC"
                     /locus_tag="AC1PNG_001011"
     CDS             complement(8461..8763)
                     /gene="gatC"
                     /locus_tag="AC1PNG_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIP6701521.1"
                     /translation="MKISEAEVRHVAKLSKLEFSDQETAEFATSLSKIVDMVELLNEV
                     DTTGVAVTTTMADRKNVLRADIAQKGESREELFKNVPETQDNFIKVPAILDGGGDA"
     gene            complement(8981..9655)
                     /locus_tag="AC1PNG_001012"
     CDS             complement(8981..9655)
                     /locus_tag="AC1PNG_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIP6701522.1"
                     /translation="MSEQFIPSVLSDLRQDIVQMPEVIKECSGIRIYGRRIRSVLFTT
                     DVSIIANHNADAILAVYPFTPSPAIIKSIMLVASVPVLAGVGGGLTTGMRSANMSLLS
                     ESEGAYAVVVNGPTDVKTIEAINKVVDIPIIYTVVSEKSDLTSRIKAGVDILNVSCGM
                     ETPRVVKKIREAFPDFPIIATGGPTDDSIRRVIEAGANAVSYTAPSNGELFKGKMEKY
                     RKHAKD"
     gene            complement(9837..10184)
                     /gene="rplS"
                     /locus_tag="AC1PNG_001013"
     CDS             complement(9837..10184)
                     /gene="rplS"
                     /locus_tag="AC1PNG_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIP6701523.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGVGVERTFPIHTPRVDKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(10742..>11404)
                     /locus_tag="AC1PNG_001014"
     CDS             complement(10742..>11404)
                     /locus_tag="AC1PNG_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSsu7 family transposase"
                     /protein_id="HIP6701524.1"
                     /translation="FVLDLSKDELSESIRQSTSKRISDKRVAYLAEKLIALANQSYCA
                     VKKTSPMLEEVRYYAKELFRLSEQRQTILDEMVELAQPLPEYDILLSIPGIAETTATS
                     IIGELGDIRRFQSANQINAFIGIDLRHYESGNFLAKEHITKRGNPYARKILFKCIHNI
                     ASASHTNPCHIADFYEKRKRQSQTTSTKPHTIASIHRLIRTMYYLIMHNKLYDYASTQ
                     NR"
BASE COUNT         3336 a         2782 c         2162 g         3124 t
ORIGIN      
        1 tattaaccaa tactcttttg ggcaaccaat atatctttta aagaaataag caaaacagca
       61 agtaaaataa acaacattcc aacaaaatca attgcataaa attgttcatt gaccaaccat
      121 actgcaaaaa agacagatgc aatcggctca atcgaggcca aaagactgcc attgacagga
      181 ccaaccatac tgacgccttt aagaaaggca gtataggcaa aaatcgtccc gactccgaca
      241 atacctaaca ggcccaggaa acttccacca tcaataggaa gacgatgctg ccaagtttgc
      301 aaaccaagcg tgactacaaa accacccatt aacatcccta aaccaatcac tgttatactg
      361 ccgtattgac ggatcaattt acctggcaaa ataatatata gcgcataagt aaaggctgaa
      421 aagattcccc tgaacaaacc gattggagtc actgccaatt catctaattt tccatgagtc
      481 gcaatcaaaa aagttcctgc tacggctaag ataattgaaa tcaactcaat ccctgatggt
      541 ttttccctat ttttcaaaca ggtataagcc aagactaaga tagggcaaag gtattgtaaa
      601 accgtagccg ttccagcatt ggtatgatag atggcttgta aataagccat ctggttaaga
      661 accagaccca acatcgcaaa aacaaaaatt cccaacaagg cttttctatt tttcaacact
      721 atcaacagct gttctttcgc tgtagcataa gccaggccaa cgagaaagaa tcccgatacc
      781 aacaaacgca gggaggtaag catatccact gaaacaccac gtgacatcaa gtactgtcca
      841 ctcactccag acaagcccca ggctatacct gctattaatg taatcagtat cccaaatctc
      901 ttttccgcca ttatatctcc ttctcacact gtgaatactt tcattatacc aaaagagcag
      961 gacaccgtcc cactctttct tgcaattatg ttgctaaacc tacaaattct acgaatcgta
     1021 gaaaggctgc ctgtccttct ggtgttcgtt tgtagacccc tgcatcttcc aatacacgcg
     1081 caaaaatttg tcccactgac tcacgaacaa cttgttcgac agtttcctct gtcacatctg
     1141 ggtgagctgt tttcaggctt tccgcccaag gctgatgata gtcagccacc tgactatctt
     1201 gacccagtaa gaatttttct acttcagcca attcggcctt caagcgtgga ggtaaaatcg
     1261 ccaagcccat tacctcaatc aagccgatgt tttctttctt tatatgttgc acatctggat
     1321 gtggatggta gatgccatct ggaaactctt ctgaggtctg attgtcgcgg agaaccaaat
     1381 ccagctcgta caaatctcct cgtttccgag caatcggggt gatggtatga tgaggagttc
     1441 catccgtctc agcctttatc tgaacgttat catctgagta gctccgccat ttttccaaaa
     1501 tcttagtcgc taaaccaaga agcgatggct tatctgccga actcaagcga atcaccgaca
     1561 taggccattt gacaattcct gccgaaacgg actcaaagcc gtcgaagacc agctggcgct
     1621 caatcggcgc cttttccatg gcaaaactgt gccgcccacc ctgatagtga ttgtgggtca
     1681 agatggagcc acccgagata ggtaaatcag agttggaacc aacaaaatag cctggcaaca
     1741 aatccaacaa gtccaagagc tgctcaaatg ttcgcggact aatgaccatc ggcacatgtt
     1801 cctgatttag gaaaatagca tgttcattgt agtaagcata gggcgaatac tggaagcccc
     1861 acttttcctg tccaagctcc aagcgaataa tgcggtggtt ggcgcgtgct ggatggttaa
     1921 tccgaccctg atagccctca ttttccatac agagtaggca tttgggataa ttgcttgctt
     1981 cagctttcgt agcagctgca atagacttgg gatccttctc aggtttagac agattgattg
     2041 tgatttccaa atccccatac tctgtcgaaa cttgataata gatatttttc gcaatcgcag
     2101 ccatcttgat atagtcattg cgcttgctca attcatagaa gtcaccaatc gcttgctctg
     2161 gactgtcctg ataggtctgc caaaaatcac gattgacctg acttggactt ggtgtcatca
     2221 aatccatcaa acaagcccca accatgtcct gctcttccag caattcaccc acaaaaccag
     2281 ttcgaactcc atgagccaat agttcatctt ttaattcaat caagtcctct aaatctgttt
     2341 gaacagtcag aacctggtct cccaccaaag ccaagacccg attgtgcagg taaagagcat
     2401 ccatttcctc aaaatcactg ttggcaataa cctgctcaac aaaacgatcc accaatccag
     2461 ccatcatttc tccaatctat tttcgagata aaacgcgaga gcctccagca atctccgcta
     2521 catagaagct tggttcatag ccaaccactt cagtataggt cttgccgaca ttttccgtga
     2581 aggcttcaac cttatccttg tggacaatgg caataccaca gccgccgaag ccagctcctg
     2641 tcatccgagc accaagaact ccttcttgct cccaagctgt gtgagccaag gtatccaatt
     2701 ctagacctgt cacttcataa tcatgctcca aagaaacatg ggaagcattg accagacgac
     2761 caaaggtagc caaatctcct tcttctaaag ccttacgagc ttgcaaggtc cgttgatttt
     2821 ctaagacagc atggcgagca cgcttgatac ggttctcatc tttaatcaaa taactatatt
     2881 ggtcaaaggt ccactcatcc aattcaccta gagtttgaat attcaagact gcattcaatt
     2941 cttccacagc tttttcacat tctgcacgac gctcattgta cttagaatct gccaattcac
     3001 ggcgtttatt ggtgttcata atgacaatca catggtcccc taaatccagc ggtaccaagt
     3061 catattccaa ggtattggta tcaaggtaaa tcgcccgatt atctgctccc ataccgatcg
     3121 caaactggtc catgatccca gaattgacac cgataaagtg attttccgtt tgtttcccaa
     3181 ttttcaccaa atcaagtcga gtcaattcaa gtccatacaa ctcttctgcg ataatgccaa
     3241 tcaagagttc caaggaagct gatgatgaca agcctgaacc atttggaatg ttaccgtaaa
     3301 caaagacttc catccctgta tcaatggcat gccctgcttc ttgcaagaat ttgagaacac
     3361 ccttggcata gttggtccag ttatctgcct tatcaaagac cagattgttc aaatccactt
     3421 cgataattcc aagttcttca aagttagcag aataaaaacg caaaagctgg tcgtcacgtt
     3481 tgcgagcagc cccgtaggtc cccaaggtaa tagccgcagg gaaaacatga ccaccattgt
     3541 agtccgtgtg ctcaccaatc aaattaatcc gacctggtga gaagaaagtc gcatctgcct
     3601 cttgaccaaa cacatcgaga aaggcctgct tgagttgttc tgtgttcata agaacctcct
     3661 agattttgta atcgttttct tttattgtac aactcaacaa tttaaaagtc aatattttta
     3721 ctaaattttt actaattttg ttttttatgc tatactagag acaaataaac aggagaagaa
     3781 catggttaca atcaaagaca tagcaaaaca agcccacctt tcctctgcca ccgtttcccg
     3841 cgttctcaag ggagacctga ccctatccgt cagccaagaa acccgcgacc gcattttcaa
     3901 cttggcacag gacctgggct acaccaaaca cctcaaaaaa caagctcccc aacaaaaagg
     3961 caccatcggt attgttcagt ggtacaccga gagcgaagag ctggctgacc tctactacta
     4021 ttctattcga gctagcatcg agcaaaccgc cagcctcctg ggctaccaaa tcatccgctc
     4081 ctttaacgac ctgacccatc ccctcctcca agaggttgac ggcatcatcg cggttggtaa
     4141 gttttcttct gggcaaatag cagagctaac caacttgtct tccaagctca tttttgtgga
     4201 ctctgataca ctgacggagg gattctcctg tatcacgacc gactttgaac actctgtaca
     4261 gaccgttatc gaccacttcc gttctcaagg tctgacagac attggcctcc tagttggaca
     4321 ggaagaaacc acagacggct accaactgcc aacagatccc cgcctgactg cctttcgtga
     4381 ctacctagac accttgggca tgctccaaga agactacatc taccaaggaa aattttccac
     4441 ccaatcagga tatgagctga tgagccaggc tattgaggac ctaggcgacc agctaccccc
     4501 tgcctttttc atggcaaatg ataccctggc agtcggagcc ctgcgtgctt tacaagagcg
     4561 tcaaatcgca gtgcccggta gagtccaact catcaccttt aacgacacag ccatcacccg
     4621 ccaggtctat ccagccctgt cttccatcag cgtcttcacc gaggaaatgg gccaggaagc
     4681 tatgcaactg ctggaccgtg tgattgcgag tccgcagccc caccatcccc gtaagattaa
     4741 gttgggtacc cagctagtaa tacgcgagag ttcttattga taaggaaatc ttccatcatt
     4801 ccgttactta tattgaattt tatacaagtg gtttgatacc gagtacggct tttcaataag
     4861 tattaaagat gttaaacttc ttgtaaaact tgtactaaaa tttgcatagc acatgctcat
     4921 agccaacatg aacagattat ttgcatttta caagtaatct ggtataatgg atatagaaaa
     4981 aggagctgga cggcaatcca gccccaaatg tagaaccgtt aaaaagacga tggctcagtt
     5041 tagattattt agaaataacc gttactgtcc aaagtttgac ggttatttct ttttgtcatt
     5101 atctttgaag attttatagc acaagccaat caatgcaata gtaaaactac caaagcctag
     5161 aatagtttgc acaacttcaa aagctgtcaa aagatgctcc tcctttccgt cagattttga
     5221 tgaattgccc ataggcatca cctcactttc agaaaatcaa gagccaccgt cttcactttt
     5281 ctacccatct agtataacat actcattcct aaaaattatt ggaatagtgc agtattcccg
     5341 aaatctttga gaacccccaa aaaattacaa ctcttctata gtgatgatat tttttcattt
     5401 tagctaacta tcctttaaca gtcaataccc gcgctacctt ctaatacttt ataccgtttt
     5461 taaaatatag cataaaaata agatagaagc acctagttta caagtaacag taaaaaccgc
     5521 taagattcaa tcctagcggt tttcttctat tcaatttaat ccaacaactt agccaattcc
     5581 tgagccaaga gttgttgtgc aacttgcggg ttagcttgtc ccttagtcgc tttcatcaag
     5641 aaaccagtaa aggccttgtc tgcattgcgt ttacctgact tgaagtcagc tacagccgct
     5701 tcgttatccg caaagacttg gtggataatc ggaatcaaga ccgcagggtc tgaaatctgt
     5761 accaaaccag ccttctcaac ataagcctta gcagagccgc cttccttagc caagtgaaca
     5821 aagactttct tagcaatttt ggatgaaata gtgccatccg caatcaaggc aatcatctca
     5881 accaagtttt ctggtgtcaa agcgatttgc tcaatagtct taccttcagc attgaggaac
     5941 tgagccacct caccctgcaa ccagttggat acctgcttag catcgccacc tgctgccact
     6001 gctgcttcaa agaagtcaga cagagccttg gttgatgtca actgaccagc atcgtaggta
     6061 gtcaagccca agttttccac atagtgagca cggcgagcct ttggaaagac tggcaattct
     6121 gcacgcactt catcaatcca gctatcatca atctcgtaga gcggtaggtc tggctctggg
     6181 aagtaacggt agtccgctga accttctttg acacgcatga gaatggtttc accagtagat
     6241 tcatcgtaac gacgcgtttc ctgctggatt tgaccacctg aacgcaagat tttggcctga
     6301 cgttctactt cgtgttgcaa gcccttgcga acatagttga aggagttgag gtttttcaac
     6361 tcagtcttgg taccaaattt ttcttgacca tagggacgaa gagagatgtt ggcatccacg
     6421 cgcatagaac cctcttccat cttcacgtct gaaataccag tgtattggat aatttccttg
     6481 agagcagtca agtaggcata ggcctcctca ggcgaacgca tatcggcttc tgatacaatc
     6541 tcaatcagtg gcactccttg acggttgagg tccacataag aataaccgtc tgtcccgtgg
     6601 gtattcttac cagcatcctc ttccaagtgg gcacgctcga tacggatttt cttggtcgaa
     6661 ccatcttcta actcaatctc aatccagcca ttgtagccaa tcggctcgtc aaactgggaa
     6721 atctgatagg ctttagggtt atcaggatag aaatagttct tacggtcaaa gtgcatgtcc
     6781 ttatgaatgt ccatgttcaa agccaaggca gccttgattc ctgcgtccac aacgccctta
     6841 ttaagaacag gaaggacacc tgggaaagac cagtcaatca cgttggtatt ggcatttgga
     6901 tcctcaccaa aatgagcaga tgaaggtgag aaaattttcg agttggtatt caactccaca
     6961 tggacttcta gaccaataat cgtttcaaag ttcattagtt agcacctcca aaaatcactg
     7021 gttgttgctt gtggtagtct gttgtcgctt caaaggcagc agccacttgg taaatggtct
     7081 cttctgaata ttttggacca atcaactgca aaccgactgg caagccttca acaaaaccag
     7141 caggaataga aagaccagga agacctgcta agtttacagg aattgttaac aagtccgcca
     7201 agtacatagc cacagggtca tggttgagcg tgtccaaacc aaaggcaacc gtcggagctg
     7261 ttggccccaa aatcaagtca tagtctgcaa agactttttc aaaatcctgg ataatcaagg
     7321 tccgtacctg accagccttc ttgaagtagg catcgtagta accagatgac aagctaaatg
     7381 tacctaacat aatacgacgt ttgacttcct caccaaaacc ttggctacgg gttttcacat
     7441 agatttcttc caagttggtc gcatcttctg cacggaaacc ataacggata ccgtcaaaac
     7501 gttgcaagtt agaagacgcc tctgatgaag caatgatgta gtaaacagca accccatact
     7561 tagaatgtgg caggctgact tcttcaatga ttgctcccaa gctttccaag tgcttagccg
     7621 ccttgagaat agtttccttc acctgcggat caatcccttc tcccatgtat tcttttggca
     7681 gagcaatctt catacccttg atgtcctgac caatcttgct agtaaagtct gcaatttcat
     7741 tgatggttga tgttgcatcc ttgacatcat gaccagagat gacattgagc aactgggcat
     7801 tttccttaac cgtctgtgag aatggaccga tctggtcaag agatgaacca aaggcaatta
     7861 aaccaaaacg tgacaccgtt ccataggtcg gtttcatacc gacgatacca ttaaaggctg
     7921 ctggttgacg gatagagcca cctgtgtcag aaccgagtga taaacggact tgaccagccg
     7981 caactgcagc agcggagcca cctgaagaac caccaggaac ttttgtctgg tcccaagcat
     8041 ttttggtcgg tttgaaggct gagttctcat tagaaccacc catagcaaac tcatccatgt
     8101 tggtcttacc aacaacaatc atatccttgg cataggcttg ggaaaccgat gtcgcatcga
     8161 aaatcggctc gtagttatag agcatttttg aagctgccgt ggtcaaaatc cccttagtag
     8221 agatattgtc cttgactgcc aaaggaatcc ctgccataac attgtccgca tcaatccctt
     8281 tttcatcaag ggcagcagcc tgcgccaagg ccgcatcttc tgtgatagtc aagaaagcat
     8341 ccactgctcc ctcacggctc ttaatgtctt ccaaagttgc cttggtcaac tcaagagccg
     8401 aaatctcctt tttgacaagg aggtcatgca attcatcaat ggttttattg ttaaaggtca
     8461 ttaggcatct cctcccccgt ctagaatagc tggtaccttg ataaagttat cttgggtctc
     8521 aggcacattt ttaaagagct cctcacggct ctcacctttt tgggcaatat ctgctcgcaa
     8581 gacattctta cggtcagcca tggtcgttgt caccgcaaca cctgtcgtat ccacttcatt
     8641 gagcaactca accatatcga caatcttgct caaacttgtc gcaaattccg ctgtttcttg
     8701 atccgaaaat tccagcttag acagcttggc aacgtgacgg acttcagctt cagaaatctt
     8761 cattcttact ttcctcctaa tggaaatcta tcttctcatt ataccatatt tccagcccca
     8821 gacaaaggta gaaatgacct aatatgttac caatttcctc ctaaaaatcc attctttaaa
     8881 ttgcgaagta ttcctaatct cacaccgtaa aacgataaaa aacagcaagt ttcatctagc
     8941 aataaaactt actgtttgct tagcctatct tttttatgag ctaatccttt gcatgtttgc
     9001 ggtatttttc cattttccct ttaaaaagct ccccattact tggtgctgta taggagacag
     9061 cattggctcc agcttcgata acccgacgaa tagagtcatc agtcggacca ccagtcgcaa
     9121 taattggaaa gtcagggaaa gcttctcgaa tcttcttcac aacccttggc gtttccatac
     9181 cgcaagaaac atttaaaata tctactccag ctttaatccg tgaggttaaa tcagactttt
     9241 cagaaacaac ggtataaatg ataggaatat ctactacctt attgattgcc tcaattgttt
     9301 ttacatccgt tggtccattt accacaacag cgtatgctcc ctccgactca gacaatagac
     9361 tcatattagc tgaacgcata cctgtcgtca agcctccacc aacaccagcc aagactggaa
     9421 ctgacgcaac caacataata cttttgataa tagctggact gggcgtaaat ggataaacag
     9481 ccaaaatagc atctgcattg tgattggcaa taatagacac atccgttgta aataagactg
     9541 aacgaatgcg acgaccataa atacgaattc cactacattc cttgataact tctggcatct
     9601 gaacaatatc ctgccgtaaa tctgataaaa cagatggaat aaactgttcg ctcatccgca
     9661 acctcctcta tactagctac aataatatat agtataacat attatttcat ggaagataca
     9721 caaaaacagt ccattttacc actgctttca caattaaata ctttttcaac agattaaaaa
     9781 aaaagacagc tttcgctgcc tcccattctc cgaccaaaat agtctgtaga actatcttaa
     9841 cgacggattt ctttgatacg tgctgcttta ccttgcaatg cacgtaggta gtacaattta
     9901 gcacgacgta ctttaccgta acgtactact tcaatcttat caacacgtgg agtgtggatt
     9961 gggaatgtac gttcaacacc tacaccacca gagattttac gtacagtgta catttctgag
    10021 atgccttgac ctttacgaga gataacaaca ccctcgaaaa tctggatacg ttcgcggtta
    10081 ccttcgacaa ccttagcgtg aacacgcaca gtgtcaccag gacggaatga agggatgtca
    10141 gtacgaagtt gaccttctgt caaactttgg atcaatggat tcattttatt ctcctatctt
    10201 tgtcaatcat aggtgctttt aaacccagcg gataaactgt atttttgtgc gtccattaca
    10261 cacagactat attatacacc aagtaatcac ctatgtaaag cattttgata aatttatcat
    10321 ggtcaatcac accgcttctt ttacaagtta agtcaacaag gtctgagttt gattttcgaa
    10381 gagtgttaat aatacagatt cctattggag aaaaatgaaa agaggctgag caaaaagacc
    10441 aacctcgctt ctcagggttc gtgtcaacat ctcagcgcag tggttgattg gcagatttgt
    10501 tcgtgtttta cactccaaat ctgacctaat caactgtgcg agggggcggg aagacgaact
    10561 ctttttctac catttgtcaa gtccatcaag gtatttgacg aaaaatattt tgagttcaat
    10621 agtcaaaata aggaaattgt tttattttgg actaaagttt agtgtaaaaa gtgcatacaa
    10681 aaccaacacc ttatgttgaa attttttgat aaggtgttac aatgatagag cataaacagt
    10741 tttaccgatt ttgggttgaa gcgtaatcgt aaagtttgtt atgcataatg aggtaataca
    10801 ttgtccgaat gagacgatgt atggaggcaa tcgtgtgcgg cttcgttgaa gtcgtttgcg
    10861 attgtctttt tcgtttctca taaaagtctg cgatatggca aggattggta tgactagctg
    10921 aagcgatatt gtggatacac ttgaacagaa tctttctagc gtagggatta ccacgcttgg
    10981 taatgtgttc cttagcgagg aagttaccag attcatagtg tctcaggtca ataccgataa
    11041 aggcattgat ttgattggct gactgaaaac ggcgaatatc tcccagttca ccaataatac
    11101 ttgttgcagt agtctcagct attccaggaa tagagagcag aatgtcatat tcaggtaatg
    11161 gctgagctag ttccaccatt tcgtctagga tagtttgtct ctgttcagaa agccgaaaca
    11221 attcttttgc atagtaacgg acctcttcca gcattggaga ggttttcttg acagcacaat
    11281 acgattgatt agctagtgct atcaacttct cagctaaata cgccacacgc ttgtccgaaa
    11341 tacgttttga ggtggactga cgaatgctct ctgagagttc gtccttgctt aaatcaagca
    11401 cgaa
//
LOCUS       DBNFEG010000056        11286 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.056, whole genome shotgun sequence.
ACCESSION   DBNFEG010000056 DBNFEG010000000
VERSION     DBNFEG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11286
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.056"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(76..1944)
                     /locus_tag="AC1PNG_001015"
     CDS             complement(76..1944)
                     /locus_tag="AC1PNG_001015"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIP6701525.1"
                     /translation="MTLVNNLKNHLHIITTSLCLLLILIGIFLLQTNQGATPILFISA
                     FVIGGYQSAKEGISELIFDKHLSVDLLMILAAIGSGLIGYWMEGALLIFIFSLSSTLE
                     ELAMEKSKNAIAALMNMTPPTARKIEENGDITVSDTAAIRIGDLLQVRKGDTVPLDAT
                     LISPQSIFDESMITGEPLPAEKAAGAAVIGGTINQGPTVTVQVTAEKGDALFDKIVQM
                     VENAQESKSQTATFIENMEDTYVKVVLVVVPLFILFAHFALGWDWLTAFYRGMILLTI
                     ASPCALVASSSPATLSAISRAARKGMIIKGGDIADNIANLEAIVFDKTGTLTIGKPEV
                     VGATYLGDEKLIKEVVQAVEKQSSHPIAQALMTYTADSSAIALQSLEDVTGKGLVAGY
                     QDDSWKIGKAGFVVDSLVSPLSADLVAQIDEAESTGKTLVYVSQNDVLVAIFMVEDSL
                     KPESKQLIAQLKEMGVTPILLTGDQEKTAHYVASQVGIERVIANCLPTDKATIIQELQ
                     TEFASVGMVGDGINDAPALAQANVSYAMGSGTDIAMESADIVLMEDLTRIPYSIRLSK
                     KMRGIIKQNIVFALSVIALLIISNLFQSINLPLGVVGHEGSTILVILNGLRLLYFK"
     gene            complement(2093..2554)
                     /locus_tag="AC1PNG_001016"
     CDS             complement(2093..2554)
                     /locus_tag="AC1PNG_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIP6701526.1"
                     /translation="MELHSHLNAENQTAFEHVIQHLKEKGVRITETRKAVVAYIIESD
                     DHPSAEMIYKDLLPNYPNMSLATVYNNLKVLLEEGFVTELKRTNDTTTYYDFMGHEHL
                     NVICEKCGKITDFMDIEIPSLKREAHEQTGYKITKEVLSVYGICPDCQFNS"
     gene            2714..3463
                     /gene="truA"
                     /locus_tag="AC1PNG_001017"
     CDS             2714..3463
                     /gene="truA"
                     /locus_tag="AC1PNG_001017"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIP6701527.1"
                     /translation="MTRYKAIISYDGHDFSGFQRQPHARTVQEEIEKTLVRLNSGQPV
                     TVHGAGRTDAGVHAYGQVIHFDLAGSRDAEKLRFALDTQTPEDIDVVSVEQVADDFHC
                     RYAKHSKTYEFLVDIGRPKNPMMRHYATFYPYDLDLSLIEEAIQDLVGTHDFTGFTAS
                     GTSVEDKVRTITAATMEYDQRRQFLIFTFSGNGFLYKQVRNMVGTLLKIGNGRMPVGQ
                     IKRILAEKDRGLAGPTAAGNGLYLKEIIYED"
     gene            3453..4214
                     /locus_tag="AC1PNG_001018"
     CDS             3453..4214
                     /locus_tag="AC1PNG_001018"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIP6701528.1"
                     /translation="MKTKYILALSGNDIFSGGGLHADLTTYAVHKLHGFVAVTCLTAM
                     TEKGFEVIPTDAAVFAQQLASLKDVPFSAIKIGLLPTVEIAEQALAFVKAHQEIPVVL
                     DPVLVCKETHDTEVSQLRDELLKFLPYVSMITPNLAEAQLLLQKDIKTVEEMKQAAVA
                     LYDFGAKAVVIKGGNRLGGPEAVDVFYDGQEIVVLQSPLLSENNVGAGCTFASSIASQ
                     LLLGQEPLEAVRLSKDFVHAAIEKSDDYGVIQYEK"
     gene            4204..4689
                     /locus_tag="AC1PNG_001019"
     CDS             4204..4689
                     /locus_tag="AC1PNG_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIP6701529.1"
                     /translation="MRNKKTQELVLLAILTALSLVLAFIHVPTPTGFFTLLDVGVFFT
                     AFYLGKKEGAIVGGLAGLLIDFLLGYPQWAFFSLLFHGAQGYFAGWTGKKRILGLVLA
                     TLSMVGGYYLASRIYFNDLKAIESVLSNTLQNTVGLVVGYILAQAIERIGAVNHVIKG
                     N"
     gene            4670..5236
                     /locus_tag="AC1PNG_001020"
     CDS             4670..5236
                     /locus_tag="AC1PNG_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HIP6701530.1"
                     /translation="MSLKEIKEQTQQVVEEVLDLSNLQKGQIFVLGLSSSEVIGGHIG
                     KNSSLEVGEVVVETILEILEPKGIYLAVQGCEHLNRALVVERELAVQKDLEMVNVLPT
                     LHAGGSGQLAAFKYMKDPVEVEFITAQAGVDIGDTAIGMHIKHVQVPIRPSLREIGQA
                     HVTALASRPKLIGGARAAYQEDKIRKGS"
     gene            5236..5856
                     /locus_tag="AC1PNG_001021"
     CDS             5236..5856
                     /locus_tag="AC1PNG_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HIP6701531.1"
                     /translation="MKEFTVKDLVWTRPPRQFTISDEEITITTHPQTDLWQKTYYHFV
                     NDNAPVLQMETEEDYFTFTVKTDFAGSHTRFDQCGIVLYQDSENWLKCSIEYENDDFQ
                     HLGSVVTNHGFSDWATTEISSQIKSMWFRLSRRGADYCFENSEDGIHFKQMRVCHFHQ
                     GAGKIAFGLYACSPENSSFTARFTNMELSDCKWQAHDGQEPDEIFR"
     gene            complement(6420..6929)
                     /locus_tag="AC1PNG_001022"
     CDS             complement(6420..6929)
                     /locus_tag="AC1PNG_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIP6701532.1"
                     /translation="MEKVKRKESLFVESVVEALWELLEDKSFEKISVSELVERAGIGR
                     VTFYRNFSNKEEVLERSFNMELQAWLSERQIDLSDWSDAHLLLALRQFFDFWYEQQDK
                     IRLLTANHLDYLLEEVLDGYFKERLGDLTDDFHLQFIVGGFFRVLKTWVARGCKESPD
                     DIMRLMGEQ"
     gene            7071..8189
                     /locus_tag="AC1PNG_001023"
     CDS             7071..8189
                     /locus_tag="AC1PNG_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HIP6701533.1"
                     /translation="MKKVWKIVAAVTVVLALLGFIAYKKTFGWSAPELVDQTPQVNEW
                     YRLSPEGVVDSQGNQAHGLLRIGKEKNKVMVYFFGGGVSINEETASGGTQYYATTTGH
                     QDYVATWGIGSPQEDNPFKDWTMIVLPYGTGDFHAGTQNFSYIDDKGKEQVVHHQGYS
                     NLMSILAAAKAHIGNPDTLLVTGFSAGGFATSLLADDVISHFPTAKNVTVAVDSSFLR
                     HDNWKSIAENVWKTPTSISDRLHSDNIVLDSLVALHEKRGDSVKILFDISYRDGILQQ
                     YQAYIDHEKLADATEESSDHFQKELKKMIQELQSKIDGVGIFIWNYGQDEKTTATQHT
                     TINFSTFFTPMAQDKSVAEWLIDAVNGQVNSYGLELID"
     gene            8199..9218
                     /locus_tag="AC1PNG_001024"
     CDS             8199..9218
                     /locus_tag="AC1PNG_001024"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIP6701534.1"
                     /translation="MKKIKKIILWILGIVASLGIIAYVSVTNHPQLVVGVIQSFMYQE
                     SSPNSYSPLYDPIDGLKENGQYLKSEIAYSKDYPNSFLDITYPDQNFEADRPTLFYFH
                     GGGFFGGSKNMGDPLAASQATYLIDDIVSKGYNVVNVDYALVPDYHFPTPVIQMNQAI
                     AYIKEHAEEYHLNMDNVVLMGSSAGAIMVAQYGSVLANQEYAQLLNIEPSIAKEHVKA
                     LVIDDAPLDYAKFSLAAKMLVGNYVSETIYLSEEEINAYNPISHVNKDYPASFLLGSE
                     YRTDMMSLNKALKQAGVENELVDPLAEEGLEKPHGFVANLRTDLVSAKAFERMITFID
                     DRTKE"
     gene            9220..9819
                     /locus_tag="AC1PNG_001025"
     CDS             9220..9819
                     /locus_tag="AC1PNG_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HIP6701535.1"
                     /translation="MNTKNFYWVREPENYQISEKEIRIITQAHTDLWQKTYYHFVNDN
                     APLLLMDTEEEYFSFTVKTDFSNSHTRFDQCGVVVYQDSENWIKGSIEYENEDFQHLG
                     SVVTNQGFSDWATQEIPASVKSIYCRLSRRGSDFCIENSKDGIHFEQMRICHLHEGGG
                     KIHFGIYACSPEDSSFEAVFTEMTLTDCKWLAHDGQQPD"
     gene            9934..11217
                     /gene="tig"
                     /locus_tag="AC1PNG_001026"
     CDS             9934..11217
                     /gene="tig"
                     /locus_tag="AC1PNG_001026"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIP6701536.1"
                     /translation="MSVSFEAKETNRGVLTFTIGQDAIKPELDRVFNKVKKDINLPGF
                     RKGHLPRAVFNQKFGEEALYQDVVNALLPAAYEAAVAEAGLEVVAQPKIDVVSMEKGQ
                     DWTITAEVVTKPEVKLGDYKNLAVSVEATKEVTDEEVDAKIEAARNNLAELVIKEGPA
                     AEGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEAQLVGHAAGEEVNVEVT
                     FPEDYQAADLAGKPALFVTKIHEVKAKEVPALDDELAKDIDEEVETLDELKAKYRKEL
                     EAAKEVAFDDAVESAALELAVENAEIVELPEEMIHEEVHRAINEFLGGMQQQGISPDM
                     YFQITGTTREDLHKQYEADAEKRTKTNLVVEAVAKAEGFEATEEEINKEIEDLAATYN
                     MEVAQVRSLLSPEMLKHDIAVKKAVEVITSTATVK"
BASE COUNT         3207 a         2335 c         2666 g         3078 t
ORIGIN      
        1 caataatttc tgaaatgcaa gcgttttttt aaaaaagaat aataagaaaa ccgccaatcg
       61 cttggcggct ttatgctatt taaaatacaa gagtcttaac ccattcaaaa tcaccaaaat
      121 cgttgatcct tcgtggccca ccacaccgag tgggaggttg atagactgga agaggttgga
      181 gatgataaga agggcaatga cagatagggc aaagacgata ttttgcttga tgatgccccg
      241 cattttctta gagagacgaa tggagtaagg aatccgtgtc agatcttcca tgagcacgat
      301 gtcggctgac tccatagcga tgtccgttcc gcttcccata gcatagctga cattggcttg
      361 ggcaagggca ggagcgtcgt tgataccgtc gcccaccata ccgacagacg caaactcggt
      421 ttgcagttct tggataatag tagccttgtc cgtcggcaag cagttggcaa tgacccgctc
      481 gataccgacc tgactcgcca cgtaatgagc agtcttttct tggtcgcccg tcaggagaat
      541 tggcgtcact cccatttctt tcaactgggc aataagctgc ttgctctcag gcttcaaact
      601 atcttccacc atgaagatcg ccacaaggac atcattttga ctaacataga ccagggtttt
      661 tccagtgctt tctgcctcat caatctgtgc aactaagtcc gcagacaggg gactgaccaa
      721 gctatctacc acaaagcccg ccttaccgat tttccagctg tcgtcctgat agcctgccac
      781 caagcctttt ccggtcacgt cttctaggct ttggagggca atagcggagc tgtcagccgt
      841 ataggtcatc agagcctgag cgatgggatg gctagattgt ttttcaactg cctgaacaac
      901 ttccttgatt agtttttcat cgccaagata ggtcgccccg acaacttcag gtttaccaat
      961 ggtcaatgtg cccgtcttat caaagacgat ggcttctaag ttggcgatat tgtccgcaat
     1021 gtcacctccc ttgataatca tgcccttgcg tgccgcccgg ctgatggcag aaagtgtcgc
     1081 cggtgaagaa gaagctacga gggcacaagg agaagcgatg gtcaagagaa tcataccacg
     1141 gtagaaggca gttaaccagt cccagcccag tgcaaaatgg gcaaagagaa tgaagagcgg
     1201 tactaccacc aaaacaacct tgacataggt atcttccata ttttcaatga aagtcgctgt
     1261 ttgtgattta gattcttggg cgttttcaac catttggacg attttatcga atagggcatc
     1321 gcctttttca gctgtaactt gaacggtcac agttggacct tggttaatgg ttccgccgat
     1381 aaccgcagca ccagctgctt tttccgcagg cagaggctcg cctgtaatca tggattcgtc
     1441 aaagatggat tgcggactga tgagggtagc atccagcggt acagtgtccc ccttacgaac
     1501 ttgcaagagg tcaccgatgc gaatggctgc cgtgtctgag acagtgatgt caccattttc
     1561 ctcgatttta cgagcagttg ggggagtcat gttcatgagg gcggcgattg cattcttgct
     1621 tttctccata gccaattctt ccaaagtaga agaaagggag aagatgaata tgagcagagc
     1681 cccttccatc cagtagccga taaggccaga gccgatagca gccaaaatca tgagcaggtc
     1741 aaccgacagg tgcttgtcga aaatcagctc agaaatgcct tccttggctg actggtagcc
     1801 cccgatgaca aaggctgaaa tgaagaggat gggtgtggct ccctgattag tttgaagtaa
     1861 gaaaatcccg atgaggatca acaacagaca gagagaagtt gtgatgatgt gaagatggtt
     1921 ttttaggttg tttacaagag tcatagttag ttccttattt ataatcatta tcgtttcgat
     1981 aagttaatta taacagttct aaatagaaaa tcaagagttt tttagaataa ttataaataa
     2041 gatttgtcag acaattcaaa gagaaaagaa aaaagcaacc agtggctgct ttctagctat
     2101 tgaattgaca gtcggggcag ataccataca cagacaagac ttccttggta atcttataac
     2161 ctgtttgttc gtgggcttcg cgtttgaggc tagggatttc gatgtccatg aagtccgtta
     2221 ttttcccgca tttttcacag atgacattga gatgttcgtg gcccatgaaa tcataatagg
     2281 ttgtcgtgtc attggtacgc ttgagttcag tgacaaatcc ttcttccaag agaactttga
     2341 ggttgttata gacagttgcc aaactcatgt taggatagtt gggcaggagg tctttgtata
     2401 tcatttctgc gcttgggtgg tcatcgcttt cgataatgta ggcaacgact gcttttctag
     2461 tttctgtaat gcgaacacct ttttccttta agtgttgaat aacatgctca aaggcagttt
     2521 ggttttctgc gttaaggtga gagtgtagtt ccattcgtgc ctcctgttct tttcaagaat
     2581 aattctaatt tacttttctt cattataatt ctttttatga aagctgtcaa acttagaagg
     2641 ggttcatctg tgctagtaca gtttttgttt tataggcttg tttttctaag tttgcttgtg
     2701 ctacaatcag aacatgacac gatataaggc aattatttcc tacgacggac atgacttttc
     2761 tggtttccaa cgccagcccc atgcccgtac agttcaggaa gaaatcgaaa aaacactagt
     2821 aagactaaac agtggccagc cagtgacagt gcatggtgca ggtcggacag acgcaggagt
     2881 gcacgcctat ggtcaggtga ttcactttga cttggctggc agtcgagatg ctgagaagct
     2941 ccgctttgcc ctagatactc aaacccctga ggatattgat gtggtcagtg tggagcaggt
     3001 ggcggatgat tttcattgcc gctatgctaa gcacagcaag acctacgagt ttctggtgga
     3061 tattgggcgg cctaagaatc ccatgatgcg ccactatgcg accttctatc cctatgattt
     3121 ggatttgagc ttgatagaag aagccattca ggacttggtg gggacccatg attttacagg
     3181 ctttacggct tcagggacct cggtcgagga caaggtgcgg accattacgg cggcgactat
     3241 ggaatacgac cagcggcggc agttcttgat ttttaccttt tcaggtaacg gttttttgta
     3301 caagcaggtg cgcaatatgg tcgggacctt actgaaaatt ggcaatggtc gcatgcctgt
     3361 gggacagatt aagcggattt tggcagaaaa agatcgtggt ttggcaggtc caactgcggc
     3421 gggcaacggc ctctatctaa aggagattat ctatgaagac taagtatatt ttagcacttt
     3481 ctggcaacga tattttcagc ggtggcggtc tccatgcgga tctgacgacc tacgctgtcc
     3541 acaagctgca tgggtttgtg gcggtgacct gcttgacagc tatgacggaa aaaggttttg
     3601 aggtcattcc gacggatgcg gcggtgtttg cccagcaact ggctagtttg aaggatgtac
     3661 ccttttctgc gattaagatt ggcttgcttc ccacggtgga aatagcagag caggctttgg
     3721 cgtttgtcaa ggctcaccag gagattcctg tggtcttgga cccagtcttg gtctgcaagg
     3781 aaacgcatga tactgaggtc agccagttgc gagatgaact cttgaaattc cttccctatg
     3841 tcagcatgat cacgccaaat ctggcagaag ctcagctatt gctccaaaaa gacatcaaaa
     3901 cggtggagga gatgaagcag gcggcagtgg ccttgtatga ctttggtgcc aaggcggtgg
     3961 tcatcaaggg cggcaatcgt ctgggtggcc cagaggcggt cgatgttttc tacgatggtc
     4021 aggaaatcgt ggtgctccag tcgcctctgt tgtcagaaaa caacgtgggg gcgggctgta
     4081 cctttgcgtc cagcattgcc agtcagcttt tgctgggtca agagcctttg gaggcggtca
     4141 gactgtccaa ggactttgtc catgcggcga ttgaaaaatc agatgattat ggggtgattc
     4201 aatatgagaa ataaaaaaac gcaggaattg gtcttgctcg ctattttaac agccttgagc
     4261 ttggttttgg cctttattca tgtgccaaca ccgacgggat tttttacttt gttggatgtg
     4321 ggtgtcttct ttaccgcctt ttacttgggc aagaaagagg gagccattgt tggcggtttg
     4381 gcagggctat tgattgactt cttgttgggc tatccccagt gggctttctt tagcctgctg
     4441 ttccacgggg ctcaaggtta ctttgcgggt tggacaggta agaaacggat tctcggttta
     4501 gtcttggcga cgctctctat ggttgggggt tattacctag catctcggat ttattttaat
     4561 gacctcaaag ccattgaaag tgttctaagc aataccttgc aaaacacagt tggtctggta
     4621 gttggttaca tcttggctca ggcaatagaa agaattggtg cagtaaatca tgtcattaaa
     4681 ggaaattaag gaacagaccc agcaggttgt cgaggaagtt ttagacctca gtaatcttca
     4741 aaagggacag atttttgtcc ttggattgtc ctctagtgaa gtcatcggag ggcacatcgg
     4801 gaaaaattcc agtctggaag ttggtgaggt ggttgttgaa accatcttgg aaattctgga
     4861 gccaaaaggt atttatctag ctgtccaagg ttgtgaacac ctcaatcgtg ccttggtggt
     4921 agagcgagaa ttggcagttc aaaaggattt ggaaatggtt aatgtcctcc caactcttca
     4981 tgcaggtggt tctggtcagt tggcggcctt caagtatatg aaggatccgg tggaagtcga
     5041 gtttatcacc gctcaggctg gtgtagacat cggcgacaca gctatcggca tgcacatcaa
     5101 gcatgtccaa gttccgatta ggcctagcct gcgtgagatc ggtcaggccc atgtcactgc
     5161 tcttgccagc cgaccaaaac taatcggcgg agcccgtgca gcttaccagg aagataagat
     5221 acggaaaggt tcataatgaa agaatttacg gtcaaggatt tagtgtggac ccgtccaccg
     5281 agacaattca ctatttcaga tgaagaaatt accattacca cccaccccca gacggatttg
     5341 tggcagaaga cctattacca ttttgtgaat gacaatgctc ctgtcttaca gatggaaaca
     5401 gaggaggact attttacctt tactgtgaag acagattttg cgggtagtca tactcgattt
     5461 gaccagtgtg gaattgtcct ttatcaagat tcggagaatt ggctaaagtg ttcgatagaa
     5521 tatgaaaatg atgacttcca acacttggga agtgtggtga ctaatcacgg cttttctgac
     5581 tgggcaacta ctgaaatcag ttcacaaatc aaatccatgt ggtttaggct cagtcgcagg
     5641 ggagctgatt actgttttga aaattccgaa gatggtatcc acttcaaaca aatgcgggtt
     5701 tgccattttc atcaaggggc ggggaaaata gcttttgggc tatatgcctg ttcaccagaa
     5761 aactccagtt ttacagctcg ctttaccaat atggaattga gcgactgcaa atggcaagcc
     5821 catgatggcc aagagccaga tgaaatattt aggtaagtag taacagaaaa atagttgaaa
     5881 ctcatactct tcgaaaatca aaatgatccg tagtcaactt gccttgaaga actcctgttc
     5941 aatcttcgcc ttcgtttcct aggcgacttt tgattttcat tgagtgtcag gggtagtggg
     6001 aaagaactcg accaagataa aaagagttcg tcaacaagtc cttatttcca gttgttgagc
     6061 tgaaacagtc tatccccaga ctgtttcact cccacccccg cacagctcaa actgtctggg
     6121 agacagtttg aggttggaaa tagagcgaac tttgttcgca acagtcgtaa tggtcagatt
     6181 tggagtgcaa aacacgaatt gctgagattt gcttcgcaaa tcctatctcc aacctttaac
     6241 agtccactgg actgttaaac ccaatcaacc actgcgctga gatgttgaca cgaactctga
     6301 gtagtggtcc tgggcttttt gcccatcctc actatacaca aaaagactta gagtgactga
     6361 aaaacagttg tctaagtctt tttttgcttg gtttggtcaa gattgtcgat aagcttgctc
     6421 tattgctcac ccatcaaccg catgatgtcg tcagggcttt ccttacagcc tctagcaacc
     6481 caggttttga gcactcggaa aaatcctcca acaatgaatt gcaggtgaaa atcatcagtc
     6541 aagtccccca gcctttcttt gaaatagcca tccaagacct cctcgagcaa gtagtccaaa
     6601 tggttggcag tcaataagcg aatcttatcc tgttgctcat accaaaagtc aaaaaattgc
     6661 cgtaaagcta gtaagagatg agcatctgac caatccgaca agtcaatctg ccgttctgac
     6721 aaccaggctt gtaactccat gttgaaggac cgttcgagca cctcttcctt attggaaaag
     6781 tttcggtaaa aggtcacccg accgatgcca gctcgctcca ccaactcaga cacactaatc
     6841 ttttcgaagg atttgtcttc cagcaactcc cacaaagcct ccacgacact ctccacaaat
     6901 aacgactctt tccgcttcac tttttccatg cgaaacactt cctttcattt gtttcctttt
     6961 gggaaaatag cagatctggg actagttttc ccactcttca tctgttatac taataaaaaa
     7021 cgaaacactt gtttccaaaa atataccata aaggacagaa aaaagaaagt atgaaaaaag
     7081 tttggaaaat agtagcggct gtaacagttg ttctagcctt gcttggtttt attgcctaca
     7141 aaaagacatt tggctggtca gctcctgagc ttgttgatca gaccccacaa gtgaatgaat
     7201 ggtaccgcct cagtcccgaa ggggttgtag attctcaagg caatcaggca cacggcctgc
     7261 ttcgtatcgg taaagagaag aataaggtga tggtctattt cttcggtggt ggtgtgagta
     7321 tcaatgaaga gactgctagt ggagggactc aatattatgc gaccacaact ggccatcaag
     7381 attatgttgc aacttgggga attggaagtc cgcaggaaga caatccattt aaggactgga
     7441 ctatgattgt ccttccttat gggacaggag attttcatgc aggtacccaa aacttttctt
     7501 atatagatga taagggaaaa gagcaggtag tccatcacca aggttattcc aatctgatga
     7561 gtattttagc agcagccaag gctcacatag gcaatccaga tacgcttctg gtaactggat
     7621 tttcagctgg aggatttgcg acctcactgc tagcggatga tgtcatttct cattttccaa
     7681 cagcgaaaaa tgtcaccgtt gcagtggaca gttccttttt gagacatgat aattggaaaa
     7741 gcattgcgga aaatgtctgg aaaacgccga cttctattag tgaccgcctg cattcagata
     7801 atattgtttt agatagctta gtagccctcc atgagaaacg cggggatagc gttaagattt
     7861 tatttgacat ttcctatcga gatggaattc ttcagcaata tcaggcctat attgaccacg
     7921 agaaactagc agatgcaaca gaagagagca gtgatcactt tcaaaaagaa cttaaaaaaa
     7981 tgattcaaga actgcaatct aagattgatg gcgtgggtat ttttatctgg aattatgggc
     8041 aagatgaaaa aactaccgct actcagcaca caaccattaa cttttcaaca ttcttcacgc
     8101 cgatggcgca agataagagt gtggcagaat ggctgattga tgccgttaac ggacaggtaa
     8161 acagttatgg attagaacta atagattagg agaacaatat gaaaaaaatc aaaaaaatca
     8221 tcttatggat ccttgggatt gtagctagtc tcggtatcat tgcctatgtg tctgttacca
     8281 atcatccaca gttggtagtc ggagtcattc aatcgtttat gtaccaggag agttcgccga
     8341 actcttacag tcccctttat gatcccattg atggtctgaa agaaaatggt cagtatttga
     8401 aaagtgagat tgcctatagc aaggactatc ctaacagctt tttagatatt acctatccag
     8461 accaaaattt tgaagctgac cgtccaaccc tcttctattt ccacggtggt ggattctttg
     8521 gagggagtaa aaatatgggg gatcctctgg cagctagcca agcgacctat ctcattgacg
     8581 atatcgtgtc aaaaggatat aatgtggtca atgttgacta tgctcttgtt ccagactatc
     8641 attttccaac gccggtgatt cagatgaatc aggctatcgc ttacataaag gaacacgctg
     8701 aagaatacca tttgaacatg gacaatgtcg tcttgatggg ttcatcagca ggtgccatca
     8761 tggtagctca atatggtagt gtcttagcca atcaggagta cgctcagcta ttgaacattg
     8821 aaccaagcat tgcaaaagaa catgtcaagg ctctggtcat cgacgatgct cccttagatt
     8881 atgccaaatt tagcttagca gccaagatgt tggttggaaa ttatgtttca gaaaccattt
     8941 atctaagtga ggaagaaatc aatgcctata acccgatttc ccatgtgaat aaagactatc
     9001 cagctagttt ccttctggga agtgagtatc ggacagatat gatgtcgctc aataaggcct
     9061 tgaagcaagc gggagttgaa aatgaattgg tcgatccttt agcagaagaa gggcttgaaa
     9121 agccacatgg ttttgtggcg aatttacgaa cagaccttgt gtctgccaaa gcatttgagc
     9181 ggatgataac ttttatagac gatagaacca aggagtaaca tgaacaccaa gaatttttac
     9241 tgggtacgag aaccagaaaa ttatcagatt tcggaaaaag aaattagaat cataacccaa
     9301 gctcatacag acttgtggca gaagacctat taccattttg tcaatgacaa tgctcccctg
     9361 ctcctgatgg atacggagga agagtatttt tcctttacgg ttaagacgga tttttccaat
     9421 agccatactc gttttgatca gtgcggtgta gtagtctatc aggattcgga aaactggatt
     9481 aagggctcta ttgagtatga aaatgaagat tttcagcatt tgggcagcgt tgtgaccaat
     9541 cagggctttt cagactgggc aactcaagaa atccctgctt ctgtcaagtc aatctactgc
     9601 cgtttgagcc gccggggcag tgatttttgc attgaaaact ccaaagacgg tattcatttt
     9661 gagcaaatgc ggatctgcca cctgcatgaa ggcggcggga aaattcattt cggtatctat
     9721 gcctgttcac cagaagactc ttcttttgaa gctgtcttta cagaaatgac cttgacagac
     9781 tgcaaatggc tagcccacga cggtcagcaa ccagattaga agcttgggca gaagctctgc
     9841 catttcccaa aagtacgtta taaaatcgtt gaaatccctg tcaattgtgt tataatgaag
     9901 agtatgcaat aaaattttaa ggagaatgac agaatgtctg tatcatttga agcaaaagaa
     9961 acaaaccgcg gtgttttgac tttcacaatc ggtcaagatg ctatcaaacc agaattggac
    10021 cgcgttttca acaaagttaa gaaagatatc aatcttccag gtttccgtaa aggtcacttg
    10081 ccacgtgccg ttttcaacca aaaatttggt gaagaagcac tttatcaaga tgttgtaaat
    10141 gcattgttac cagcagctta tgaagcagcg gttgcagaag ctggtcttga agtagttgca
    10201 caaccaaaaa tcgacgttgt gtctatggaa aaaggtcaag actggacaat cactgcggaa
    10261 gttgtgacaa aacctgaagt aaaattgggt gactataaaa acttggcagt ttcagtagaa
    10321 gctactaaag aagtaacaga tgaagaagtt gatgcaaaaa tcgaagctgc acgcaacaac
    10381 ttggctgaat tggttatcaa agaaggtcca gcagctgaag gcgatacagt tgttattgac
    10441 tttgtaggtt caatcgacgg cgttgaattt gacggcggta aaggtgagaa cttctcactt
    10501 ggacttggtt caggtcaatt catcccaggt tttgaagcac aattggtagg ccacgcagct
    10561 ggtgaagaag taaatgttga agtaactttc ccagaagact atcaagcagc tgaccttgct
    10621 ggtaaaccag ccctcttcgt aacaaaaatc cacgaagtaa aagcaaaaga agttccagcc
    10681 ttggatgacg aattggcaaa agacatcgac gaagaagtgg aaacacttga tgagttgaaa
    10741 gctaaatacc gtaaagaatt ggaagctgca aaagaagttg catttgacga tgcagttgaa
    10801 tcagcagctc ttgaattggc tgtagaaaac gctgaaatcg ttgaattgcc agaagaaatg
    10861 attcacgaag aagttcaccg tgcaatcaat gaattcttgg gtggtatgca acaacaaggt
    10921 atctcaccag atatgtactt ccaaatcact ggtacaactc gtgaagacct tcataaacaa
    10981 tacgaagcag atgctgaaaa acgtactaag acaaacttgg ttgttgaagc agtagcgaaa
    11041 gcagaaggtt tcgaagcaac tgaagaggaa atcaacaaag aaatcgaaga cttggcagca
    11101 acttacaaca tggaagtcgc acaagtacgt agcttgcttt caccagaaat gcttaaacac
    11161 gacatcgctg ttaagaaagc tgtagaagtg atcacaagca ctgcaactgt aaaataatgc
    11221 aaattggaaa ctcgacttgg tcgggttttt ctttttgttt tttgataaat tagtatatat
    11281 ttttaa
//
LOCUS       DBNFEG010000057        11226 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.057, whole genome shotgun sequence.
ACCESSION   DBNFEG010000057 DBNFEG010000000
VERSION     DBNFEG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11226
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.057"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            77..892
                     /locus_tag="AC1PNG_001027"
     CDS             77..892
                     /locus_tag="AC1PNG_001027"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HIP6701537.1"
                     /translation="MNQQFSLDQFSLKGKIALVTGASYGIGFAIASAYAKAGATIVFN
                     DINQELVDRGMAAYRAAGINAHGYVCDVTDEAGIQAMVAQIESEVGIIDILVNNAGII
                     RRVPMIEMTAAQFRQVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRETV
                     SAYAAAKGGLKMLTKNIASEYGEANIQCNGIGPGYIATPQTAPLRELQKDGSRHPFDQ
                     FIIAKTPAARWGEAEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPEA"
     gene            944..1585
                     /locus_tag="AC1PNG_001028"
     CDS             944..1585
                     /locus_tag="AC1PNG_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HIP6701538.1"
                     /translation="MKIALINENSQAAKNSLIFETLKSVTDAKGYHVFNYGMYGKEGE
                     SQLTYVQNGLLASILLVSKAADFVITGCGTGVGAMLAANSFPGVVCGFAVDPTDAYLF
                     SQINGGNALSLPFAKGFGWGAELNLKLLFERLFAEEMGGGYPRERVIPEQRNARILNE
                     MKAVTYKDLLTVLQEIDQDFLKETISGEHFQEYFFANCQDESIAAYLKTVLES"
     gene            1602..2603
                     /locus_tag="AC1PNG_001029"
     CDS             1602..2603
                     /locus_tag="AC1PNG_001029"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HIP6701539.1"
                     /translation="MKKVLLIGEPLIRITPTNYQELTDGVESRVFFGGSEINVACNLQ
                     GFGCETKLLTALPQGSLGDRFLDFLDGKGIDTSSVQRKGERVGVYYMEAGFGCRSSQV
                     YYDRGQSSLEAIQVEHFDMEQLFLEVGLVHFSGITVAIGSAVRAWLVQVLKEAKRRKI
                     PISMDLNWRSKMIGLRDAKKVFSQFATYADYCFGLEPIMANEKDWELFERDTATVHTI
                     RERMQTLKEAYKFQAIFHTIRQTDELGRNLYKAYGLADEFCCSQELKTQVLQRVGSGD
                     AFVAGALYKLLEGTSLKAVLDFAVASGTYKCSIEGDHMRQSLSKVESLLEGKNDIIR"
     gene            2614..3255
                     /locus_tag="AC1PNG_001030"
     CDS             2614..3255
                     /locus_tag="AC1PNG_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIP6701540.1"
                     /translation="MSPSQIISKLKEQGLVVVVRGDDKEVGFKTAQACIAGGITAIEV
                     AYTNPDANMIITELSRCHKDDVSVLIGAGTVLDSVTARQAIMAGASYIVSPSFNIEVA
                     KICHLYAVPYLPGCMTLTEITNALEAGCEMVKLFPGSVLGTNYITAIKGPLPHVSIMV
                     TGGVKLDNVSEWFNAGATVLGIGGEFNQLATKGEFGTIQELASSYASVRDKMK"
     gene            3541..3975
                     /locus_tag="AC1PNG_001031"
     CDS             3541..3975
                     /locus_tag="AC1PNG_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIP6701541.1"
                     /translation="MIKVIVVAHGQFASGILTSLKLIAGEVENIEAIDFSEGMSAQEL
                     KARIKSAILGEREVLILTDLLGGTPFKVSVELAAEQKEQNVVVLSGLNLAMILEANFS
                     RLTDDLEQLVGKLITTSKDGIVDSGSLLAEDNHDEELFEDGI"
     gene            3987..5177
                     /locus_tag="AC1PNG_001032"
     CDS             3987..5177
                     /locus_tag="AC1PNG_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HIP6701542.1"
                     /translation="MIKDVLIESIAKKREFRNSTLLSKKEVEAAIELAIQQLVINCEY
                     FGESFPTPATFDNHYPKMDNTEWTNGFWTGELWLGHEYSLNNEMKALAKKNTTSFLER
                     VKQRIELDHHDLGFLYSPSTVADYKLTGSKEALEASLLAADKLMERYQEKGGFIQAWG
                     QLGKAEDYRLIIDCLLNLQLLFFAYEETGEQKYYDVALSHFYASVNTVIRDDASTFHT
                     YYFDPETGKPLKGVTRQGFSDDSSWARGQSWGIYGIPLAYRKIKDPACLNLFKGVTNY
                     FLNRLPEDNVSYWDLIFNDGSGHARDSSATAIAVCGIHEMLKYLPEVDEDKETYKYAM
                     HTMLRSLIDNYANRELAPGRPLLLHGVYSWHSGKGVNEGNTWGDYYYLEALLRFYKDW
                     ELYW"
     gene            5187..5678
                     /locus_tag="AC1PNG_001033"
     CDS             5187..5678
                     /locus_tag="AC1PNG_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIP6701543.1"
                     /translation="MATPNIVMARIDERLIHGQGQLWVKSLGCNTVIVANDKVSEDKI
                     QQTLMKTVIPDSVAMRFFSLQKVIDVIHKANPAQTIFLVIKDLQDALTLVKGGVPITE
                     LNIGNIHNAPGKTQVTRSIFFGSEDKAIVRELNDVYKIRFNTKTTPSGNDGAVEVNIL
                     DYI"
     gene            5698..6474
                     /locus_tag="AC1PNG_001034"
     CDS             5698..6474
                     /locus_tag="AC1PNG_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HIP6701544.1"
                     /translation="MTISLIQAALIGLWTAFCFSGLLLGLYTNRCIVLSFGVGIILGD
                     LPTALAMGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVTPEAALALST
                     PIAVGIQFLQTSAYTLHAGAPESAIKHLRAGNLSKFKRVANGTVITFAFLGFALGFLG
                     AYSMDTLLKLVEMIPPVLLTGLSVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLQLPVIGIAIVGSIFALKEFFNKPEQVAVKQEEAHDDWI"
     gene            6461..7276
                     /locus_tag="AC1PNG_001035"
     CDS             6461..7276
                     /locus_tag="AC1PNG_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIP6701545.1"
                     /translation="MTGSKLTKKDYLNTSLRAFFLQNGFNYSNYQGIGYANVMYPAFK
                     KHFGDDKEGLTKALEENCEFYNTNPHFLPFITSLHLVMLENDLPEEETRGIKMALMGP
                     LAGIGDSLSQFVLAPLLSTIAASLAGQGLVMGPILFFLGMNIILTSIKLLTGLYGYKF
                     GTSVIDKLSDQMATISKVANIIGVTVISGLAVTFVKISVPITFAAGELTEGADQKIVT
                     IQGMLDKIAPALLPALFTILVYYLIKNKKWTTYKLVILTVIIGILGSWLGILA"
     gene            7283..7591
                     /locus_tag="AC1PNG_001036"
     CDS             7283..7591
                     /locus_tag="AC1PNG_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIP6701546.1"
                     /translation="MEQQTLYYVLIILVALSTILPVLTSYINRKRQQKALEEQLEKRQ
                     RYLSTLQAGDEVILFSGLHGKIIKLTDSLVELQIAEGVLVYVEKESIMGKAKELLFTK
                     "
     gene            7688..11182
                     /gene="hylA"
                     /locus_tag="AC1PNG_001037"
     CDS             7688..11182
                     /gene="hylA"
                     /locus_tag="AC1PNG_001037"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HIP6701547.1"
                     /translation="MGFFISQSKQHYGIRKYKVGVCSALIALSILGTRVAANQLPSAE
                     TASPQSSQLVETTPETTEAVMTSEVSSEVSPVTSTETQPSSTTAETLASPQAVQATKE
                     EKNLVANGEFISTTAPSGNWKELAATNWETWIPANVKKENGQVRIDEGRLHISSTASY
                     RVAVHQTVDVDPNKRYLFSYDVETKDLKGSGVRVRLRSLTAEGKDLSPQEFAYTPYKN
                     GSQAEHIEQILTVSPETRKLKVELFFENSVGQAWLDNISLVEYVEKTPETPEQSPELV
                     QPEIGQISLASNKVYLPVRPDLTYRIADAAVATVEKNMIRPLAAGKTQVDVYDKDTKL
                     SSFELIVTEHQATVFDTLRNNWEDISLANKRYQSNDAQMKAFLGRLDAGVASSLEKWV
                     EPTEQSKTIFNDIDFSKSSHLTTVYRRLEQMAQVVENPDSAYYHDRSLIDLVRKGMNW
                     LYTNVYNENKSIDGNWWDYEIGTPRAVVNTLIYMHPYFSQEEILTYTKPISKFVPDPT
                     TIRKTLTNPVPAVGGNQTDLSKVAILEGALREDADRVRAGAQGLTTIMKFVDKGEGFY
                     RDGSFIDHTNVAYTGAYGNVLIEGFSQLLPVIQPTEFALKEEQTNILYEWIEKAFMPI
                     LVRGELMDMTRGRSISRATGESHVQAMEILRSLVRIAESAQPEQKTKLLSFVKAQLTS
                     DTFYDSYRSLKSYKDIDLVNKLLADNQIPAEVDKDYIAAFNNMDKFVYRSAQEGFTFA
                     LSMYSSRTQNYEDMNNENRKGWYTADGMVYLYNDDLSHYSNHYWATVDPYRLPGTTTT
                     KDKREDGSGEVTLASDFVGASQLGNRLATIAMDFNNWNNSLTARKAWIVLGNKIVFLG
                     TDIQHQSAQGAETTIENRKLLTGEKYSYYINGQPVDLSKEVVTDKTQSFYMTNGKDNQ
                     SIGYVFLNQLPTHAKLDQRTGKWSDINYNQSKEEVSNSFVSLWHEHAQTSSNYAYVLV
                     PNQSMEKVNQAAASVKLLHQDRDLQVVYDQEQNVWGVVKYTDTAYKLTDDITLTDAGL
                     YTIQKVEGGYRIAFYNPSTRTVKNGIELTKAGSSLTVEMEPTAAYPSTVWKVTMPEGS
                     DKQTGSVEKTEKEEKQLKENQPSSEVKQVVHHAAEKTKPSKPRLPQTGEEASLGLGFL
                     GLLTLGAVVDFKCRRSHS"
BASE COUNT         3406 a         1972 c         2628 g         3220 t
ORIGIN      
        1 ttgacaaaac cggtttacta tattacaata caatttgtaa acgctttaca aatcaaaata
       61 caaaggagtt acgaatatga atcagcaatt ttcattggat caattttctc taaagggaaa
      121 aattgctttg gtaacagggg cgtcgtatgg gattggcttt gcgattgcat cggcctatgc
      181 aaaagcaggg gcgacaattg tttttaatga tattaatcaa gagttggttg ataggggaat
      241 ggcagcctat agggcagctg gtattaatgc gcatggctat gtttgcgatg taactgatga
      301 agctggaata caggctatgg ttgctcaaat tgaatcagag gttggaatta ttgatattct
      361 agtgaataat gctggtatta ttcgtagagt tccaatgatt gaaatgacag cggctcaatt
      421 tcgccaagtt attgatattg acctaaatgc tccgtttatt gtttctaaag cggttattcc
      481 atctatgatt aaaaaggggc atgggaagat tatcaatatt tgttccatga tgagtgagtt
      541 aggacgagaa acagtgtctg cttatgcagc ggctaagggt gggcttaaga tgttgacgaa
      601 gaatattgcg tcggaatacg gtgaagccaa tatacaatgt aacggcattg gtcctgggta
      661 tattgcaact ccacaaacag ctccgttacg tgaactccag aaggatggtt ctcgtcatcc
      721 atttgatcaa tttattatcg cgaagacccc tgcagctcgt tggggtgaag ctgaggattt
      781 gatgggtcca gcagtctttt tagcaagtga tgctagtaat tttgtgaatg ggcacattct
      841 ttatgttgat gggggaattc tagcttacat tggtaaacag ccggaagcgt aataaacgaa
      901 ttaacaaatt taacaaattt tgatataaga agggaaaaaa acgatgaaaa ttgcacttat
      961 taatgaaaat agtcaggctg ctaaaaatag tcttattttt gaaacattga agtcagtaac
     1021 agatgctaaa gggtatcatg tttttaacta tggaatgtat ggtaaagagg gagaaagtca
     1081 gttgacttat gtacaaaatg gtctgttggc ttcaatcctt ctagttagta aggcagcgga
     1141 ttttgttatt acaggttgtg gcacgggggt aggtgccatg ttggcggcta atagttttcc
     1201 aggggtagtt tgtggcttcg cggtagatcc gacggatgca tacctctttt cacagataaa
     1261 tggaggaaat gctttgtctc tgccatttgc aaagggattc ggttggggag ctgaattaaa
     1321 tctcaaatta ctttttgagc gactgtttgc agaggaaatg gggggcggat atccccgtga
     1381 acgcgttatt ccggaacaac gaaatgctcg tattttgaat gaaatgaaag cagtcaccta
     1441 taaagactta ctgacagtac ttcaggaaat tgatcaagat tttttgaaag aaactatttc
     1501 aggtgaacat ttccaagaat atttctttgc caactgtcag gacgagtcaa ttgcagcata
     1561 tttaaaaaca gtattagaaa gttaaagagt ggggggcggt tatgaagaag gtattactga
     1621 ttggtgaacc tttaattcgc attacaccaa caaattatca agaattgaca gatggtgtag
     1681 aaagtcgtgt gttttttgga ggttcagaaa tcaatgtcgc ttgtaatctt caaggatttg
     1741 gatgtgaaac caagcttttg accgcactac cacaaggctc tctaggtgat agatttttag
     1801 atttcttaga tgggaaaggg attgatactt ccagtgttca acgtaagggg gaacgtgtgg
     1861 gggtatacta catggaagca ggatttggtt gccgttctag tcaagtctac tatgatcgtg
     1921 gccagtctag tttggaagct attcaagtcg aacattttga tatggaacaa ttatttttag
     1981 aggttgggtt ggttcacttt agtggtatca ccgtggctat tggttcagct gttagggcat
     2041 ggcttgttca agtattaaaa gaagcaaagc gtcgtaaaat tccgatttcg atggatctta
     2101 attggcgttc taagatgatt ggattaagag atgcaaaaaa agtgttctcg caatttgcga
     2161 cctatgcaga ctattgtttt ggtctggaac ctattatggc taatgaaaaa gattgggaat
     2221 tatttgaaag agatacggct actgtccata caataagaga acggatgcaa actttaaagg
     2281 aagcctataa gttccaagca attttccata cgattaggca gacagatgaa ctaggcagaa
     2341 atttgtataa ggcttacgga ttggctgatg aattttgttg ctctcaagaa ttgaaaactc
     2401 aggtgttaca acgtgttggc agtggtgatg cgtttgttgc tggggcatta tataaattgt
     2461 tagaggggac atcgttaaag gctgttttag attttgcagt tgctagcggg acttataaat
     2521 gcagcataga aggggatcac atgcggcaat ctcttagcaa agttgagtct ttattagagg
     2581 ggaaaaatga cattatacga taaaggagaa aaaatgagtc catcacaaat tatttcaaaa
     2641 ttgaaggagc aagggcttgt tgtagtcgtt agaggagatg acaaagaagt cggatttaag
     2701 acggcacagg cctgtattgc aggaggaatt acagctatag aggttgctta tacaaatcct
     2761 gacgcgaata tgattattac agaacttagt cgatgccata aagatgatgt ttctgtttta
     2821 attggggctg gaacggttct agattctgta acggcacggc aagccattat ggcaggagca
     2881 agttatattg tttcaccatc ttttaatata gaagttgcaa aaatctgtca tctttatgca
     2941 gtgccatatc ttccgggctg tatgacttta acggagatta caaatgcttt ggaagcaggc
     3001 tgtgaaatgg ttaaactgtt tcctggtagt gttttgggga caaactatat tactgcaatc
     3061 aaaggaccat taccgcatgt ttcaatcatg gtaaccggtg gagtaaagct tgataatgtt
     3121 tcagaatggt ttaacgctgg tgcaactgtt cttggtattg gtggggaatt taatcaatta
     3181 gctactaaag gagagttcgg tacaattcaa gaattagcgt cctcttatgc aagtgttaga
     3241 gataagatga agtagtttat ttcaaaataa ggactgtatt tttaacgttt tcactttctc
     3301 ctgcctccaa cggggttgaa tgtgggagaa agtgagatgt tacaggggaa attttgaacg
     3361 gaaacatgga gatatttggt actcctgttt atgaaaagag ggagctggag aatatttttt
     3421 aaataaaagt ccaacaaaat ataaaacgct tacatttttt ataaaatcta ttgacaagtg
     3481 gttgtcaata gtctacaata caaagtgtag gtaaaccggt aaagttaaaa ggagagaatt
     3541 atgattaaag tgattgtcgt tgctcatgga cagtttgcta gtgggattct tacttcacta
     3601 aagttaattg caggagaggt ggagaatatt gaggcaattg attttagtga aggaatgtcg
     3661 gcgcaagaac taaaagcacg catcaaatca gctatattag gcgaaagaga agttttgatt
     3721 ttaactgatt tactaggagg tacgcctttt aaagttagtg tagaattagc agcagaacag
     3781 aaagagcaga atgttgtcgt tctttcaggt ttaaatcttg cgatgatttt ggaagcgaat
     3841 ttttcacggt taacagatga tttagaacaa ttggttggta aattgattac cacctctaaa
     3901 gatggaatcg ttgattcggg cagtctgtta gcagaggata accatgatga agagttattt
     3961 gaagatggta tatgagggag aaatgcatga ttaaggatgt tttgattgag tcaattgcta
     4021 aaaaaagaga gtttagaaat tccactctat tgagtaagaa agaagttgag gctgcaatag
     4081 aattggcgat tcaacaactt gttatcaatt gtgaatattt tggtgagtcg ttcccgactc
     4141 cggccacatt tgataaccat tatccaaaga tggataatac agagtggaca aatggttttt
     4201 ggacgggcga gctttggtta ggacatgagt attctttgaa caatgaaatg aaagcgttag
     4261 caaaaaagaa tacgacatct ttcttggaac gtgtcaaaca aagaattgaa ttagatcatc
     4321 atgatttggg atttctgtat tcaccatcaa cggtagctga ttataaattg acaggtagta
     4381 aagaggcttt agaggcttcg ctcttggctg ctgataaatt aatggagcgc tatcaagaaa
     4441 aaggaggttt tattcaggcc tgggggcaac ttggtaaagc agaagattat cggttgatta
     4501 ttgattgttt gctgaattta caattacttt tctttgctta tgaggagaca ggtgagcaga
     4561 aatattatga tgtagctctt agtcatttct acgcttctgt taatacagtt attcgagatg
     4621 atgcttcaac atttcatacc tattactttg accctgaaac aggaaaaccg ctcaaagggg
     4681 ttacccgaca aggatttagt gatgactctt cctgggcgcg tggtcaatct tggggtattt
     4741 acggtattcc gttagcttac cgtaaaatta aagatcccgc atgtttaaat ttgtttaaag
     4801 gagtaaccaa ctactttctt aatcgtttgc cagaggataa cgtttcctat tgggatttaa
     4861 tttttaatga tggtagtggg cacgctcgtg attcttctgc gacagcaata gcggtttgtg
     4921 gtattcacga gatgcttaaa tatttgccag aagttgatga ggacaaggaa acttataaat
     4981 atgctatgca cacgatgcta cggtccttga ttgataacta tgctaatcgt gagttagctc
     5041 caggtcgtcc attgctttta catggggtct actcttggca ttcaggaaaa ggtgtcaatg
     5101 aaggaaatac ttggggcgat tattattact tagaagcttt gcttcgtttt tataaagatt
     5161 gggaattata ttggtaggag gttaaaatgg caacaccaaa tattgtaatg gctcgtattg
     5221 atgagcgact aattcatgga cagggacaac tatgggttaa atccttaggt tgtaatactg
     5281 tcatcgtggc aaatgataag gttagtgaag ataagattca gcagacatta atgaaaacgg
     5341 ttattccaga ttcagtagct atgcgtttct tctctttgca gaaagtgatt gatgtgattc
     5401 ataaggctaa tcctgctcaa actattttct tagttattaa agatttgcaa gatgcactaa
     5461 ctttggttaa agggggagta ccaatcacag aattaaatat tggaaatatt cataatgcgc
     5521 ctggaaaaac gcaagtaaca cggtctatct tttttggttc agaagataag gctatagtga
     5581 gagagttgaa cgatgtttat aagattcgct tcaacacaaa gacaacgcct tctggtaatg
     5641 atggggcagt tgaagttaat atattggatt atatttaaaa aaataaggag aattatcatg
     5701 acgatcagtc taattcaagc agctcttatt ggtctgtgga cagctttctg ttttagcggt
     5761 ctattattgg ggctttacac aaaccgatgt atcgtgcttt cttttggggt cggaatcatt
     5821 ttaggagatc taccaacggc tttagctatg ggcgctatca gtgagctggc atacatggga
     5881 tttggtgttg gtgctggggg gaccgttcct ccaaatccaa tcggacctgg tatttttggt
     5941 actttgatgg ctattacaag tgctggaaaa gtaacaccag aagcagcttt agcactttca
     6001 acacctattg cggtaggtat tcaattctta caaacttcag catatacttt acatgcgggt
     6061 gctccagaat cagctatcaa acatcttcgt gcaggaaacc tttctaaatt taaacgtgtt
     6121 gcaaatggta cagtgattac tttcgccttt ttaggatttg ctcttggttt cttgggtgcc
     6181 tattcaatgg atactctttt gaaattggtt gaaatgattc caccagtatt attaaccgga
     6241 ttgagtgtag caggtaagat gcttccagct atcggttttg caatgatttt gtctgtaatg
     6301 gctaagaaag aattgatccc gtttgtattg cttggctatg tttgtgcagc ttatctacaa
     6361 ctgcctgtaa ttggtatcgc aattgtcggt agtatctttg cacttaaaga attctttaat
     6421 aaaccagaac aagtggcagt gaaacaggag gaagcacacg atgactggat ctaaactaac
     6481 taaaaaagat tatctcaaca cttctttacg ggccttcttc ttgcagaatg gtttcaatta
     6541 cagtaattat caagggattg gctatgcaaa tgtcatgtat ccagctttta agaagcattt
     6601 tggtgacgac aaagaaggat taactaaagc tttagaagaa aactgtgagt tttacaatac
     6661 caatccacat tttcttccat tcattactag tcttcatttg gtcatgttag aaaatgatct
     6721 cccagaagaa gagactcgtg ggattaagat ggctctgatg gggccgttgg caggaattgg
     6781 tgactcactt tcacaatttg tattagcccc acttttatct acgattgctg cctctctagc
     6841 aggtcaagga ctggttatgg gaccgattct tttcttcctt ggaatgaaca ttatcttgac
     6901 ctctatcaag ttgttgactg gtttgtatgg ctataaattt ggtaccagcg tgattgataa
     6961 gttgagtgac caaatggcaa caatttctaa ggtagccaat atcatcggtg tgacagttat
     7021 ctctggttta gcagtaacct ttgtaaaaat ttcggtgcca atcacctttg cggcgggtga
     7081 attaacagaa ggagcagatc agaaaattgt aaccatccag ggaatgttag acaagattgc
     7141 ccctgcgttg cttccagcct tgtttacgat tttggtttat tatctcatca agaacaagaa
     7201 atggacaacc tacaaactag ttatcctgac agttatcatc ggtattttgg gtagctggct
     7261 gggcatcctt gcataagcgc ctatggaaca acaaactctc tactatgtac taattatttt
     7321 agtagctcta tcgaccatac ttcctgtact cacttcttat atcaatcgta agcgacaaca
     7381 aaaggcactt gaggagcaac tggaaaaacg ccaacgctac ttatcaacct tacaggctgg
     7441 agatgaggtt atcctcttct ctgggctgca tgggaaaatc atcaaactaa cagacagctt
     7501 ggtagaattg cagattgcag aaggtgttct tgtttacgtt gaaaaagagt cgattatggg
     7561 aaaagccaaa gaactacttt ttacgaagta gttcttcttt cttgcctccg caaggaagcg
     7621 gttgcaggat taaaattata cccctgggac attgttttgc cccgcataaa aaggaaagga
     7681 aacaccaatg ggatttttta tcagtcaaag caagcagcat tacggcatcc ggaaatataa
     7741 ggtgggtgtc tgctccgccc ttatcgcgct gtctatcttg ggaacaaggg ttgcagccaa
     7801 tcaactgcca agtgcagaaa cagctagtcc acaaagcagt cagttagtgg aaacaactcc
     7861 agaaacgaca gaagcagtaa tgacaagcga ggtcagctct gaggttagtc ctgttacatc
     7921 aacagaaacc cagccttcaa gcaccactgc agaaactctt gccagtccgc aagctgttca
     7981 ggcgacaaaa gaagagaaga acctagtagc caatggggag ttcatcagta caacggcacc
     8041 atcaggaaac tggaaagaat tggcagctac aaactgggaa acatggattc ctgcaaatgt
     8101 gaagaaagaa aacggacagg ttcgcattga tgagggacgg ctgcatatct cttcaacagc
     8161 tagctatcga gtggcagtgc accaaacagt ggatgtagac cctaacaagc gctatctctt
     8221 tagctatgat gtagaaacca aggacctgaa ggggagcggt gttcgtgttc gcttgcgcag
     8281 tttgactgca gaaggcaagg acttgagtcc acaagaattt gcctatacac cttataaaaa
     8341 tggaagtcag gcggagcaca tagagcagat cttgacggta tctccggaaa ctcgtaaatt
     8401 gaaggtggaa ctgttttttg agaatagtgt cggccaagcc tggctggata acatctcgct
     8461 ggtcgaatac gtagaaaaga caccagaaac accagagcaa agcccagaac ttgtccaacc
     8521 tgaaataggg caaataagtc ttgccagcaa caaggtctac ctgcctgttc ggccagacct
     8581 gacctatcgg attgcggatg cggctgtggc cacagtagag aaaaatatga ttcgtccgct
     8641 tgcggctgga aagactcagg tagatgtcta tgataaggac accaagctat ctagtttcga
     8701 gctgatagtt acagagcatc aggcaacagt ctttgatacc ttaaggaaca actgggaaga
     8761 tatttcttta gccaataaac gctaccaatc taacgatgct cagatgaaag cctttctagg
     8821 gcgtttagat gccggtgtag cttcttctct tgaaaaatgg gttgaaccaa cagagcagag
     8881 caaaacgatt ttcaacgata ttgactttag taaatccagc catctgacaa cagtctatcg
     8941 ccgcttggag cagatggctc aagtagttga gaatccagac tcagcctatt accacgaccg
     9001 ctcacttatc gatttggtca gaaaaggaat gaactggctt tacaccaatg tttataatga
     9061 aaataagtca attgatggga attggtggga ttatgaaatt ggaactccgc gtgcggttgt
     9121 caacaccttg atctacatgc atccctactt tagtcaggag gagattctaa cctataccaa
     9181 accgatttca aaatttgtac cagatccaac cactatccgt aaaacattga ccaatccagt
     9241 cccagcagtt ggcggtaatc aaacggattt gagcaaggtg gccatcttgg aaggagctct
     9301 tcgcgaagat gctgaccgtg tgagggctgg tgcgcaaggt ttgacaacga ttatgaaatt
     9361 tgtagacaag ggagagggat tctacaggga tggctccttt atcgaccaca ccaatgttgc
     9421 ctacacaggg gcgtacggaa atgtcctcat tgaaggtttt tcacagttac taccagttat
     9481 tcagccaacg gagtttgccc tcaaggaaga acagaccaac attctttatg aatggattga
     9541 aaaagccttt atgccaatat tggttcgcgg tgaactgatg gatatgaccc gcggtcgttc
     9601 catcagtcgg gcaactggtg aatcacatgt tcaggccatg gagattttaa ggtctttggt
     9661 ccgcattgca gaatcagctc agcctgagca aaaaaccaaa cttctctcct ttgttaaggc
     9721 acagctgacc tctgatacct tctatgacag ctaccgcagc ctcaaatcct ataaggatat
     9781 tgatttggtc aacaagcttt tagctgataa tcaaatccct gctgaagtgg ataaagatta
     9841 tattgcagcc ttcaacaata tggataagtt tgtttatcga agtgctcaag aaggctttac
     9901 ctttgccttg tctatgtact cttctagaac tcaaaactat gaagatatga acaatgaaaa
     9961 ccgcaagggt tggtatacag cagatggcat ggtttacctc tacaatgatg atttgagtca
    10021 ttacagtaac cactactggg caacagttga cccgtatcgc ttaccgggaa cgacaacgac
    10081 caaggacaaa cgagaagatg gcagtggcga ggtaactctt gcaagtgact ttgttggagc
    10141 aagccagtta ggcaaccgct tagcgacgat tgcaatggat ttcaacaact ggaacaatag
    10201 tctgactgct cgtaaggctt ggattgttct gggaaataag attgttttcc taggtactga
    10261 tatacagcat caatctgctc aaggcgccga gacaacgatt gaaaaccgta aactcttgac
    10321 cggagaaaaa tatagctatt acataaatgg ccagccagtt gatttgtcca aagaagtagt
    10381 gacagataag actcaatcct tttacatgac caatggcaag gacaaccagt ccatcggtta
    10441 cgtcttcttg aaccaactgc cgacccatgc caaattagac cagcgaactg gcaaatggag
    10501 tgacatcaac tacaaccagt cgaaagaaga agtgagcaat tcctttgtta gcctatggca
    10561 tgaacatgca cagacttcaa gtaactatgc ttacgtcctt gttccaaacc aatctatgga
    10621 aaaagtgaat caggcagctg ctagtgtgaa attgcttcat caagacaggg atttgcaagt
    10681 ggtatacgac caagaacaga atgtctgggg cgttgtgaaa tacacggata cagcctacaa
    10741 gctgacagat gatattacct taacagatgc cggtttgtat accattcaga aagtcgaagg
    10801 tggctatcgc atcgcctttt acaatccgtc tacccgaacc gtaaagaatg gcattgagtt
    10861 gacaaaggca ggttctagtc tgactgttga gatggagccg actgcggctt atccatccac
    10921 tgtctggaag gttactatgc cagaaggttc tgataaacaa actggaagtg tcgaaaaaac
    10981 agaaaaagaa gaaaaacaac tcaaggaaaa ccaaccaagt tcagaggtga aacaagtggt
    11041 tcatcatgct gccgaaaaaa caaagccgag taagccccgt ctgccccaga caggagaaga
    11101 agccagtctg ggtcttggtt tcttaggttt gctaaccttg ggggcagtgg tggatttcaa
    11161 gtgtcgcaga tcgcactcct aaatgaaatt cgtgggtgaa gggggagcgg gaaagaactc
    11221 gactag
//
LOCUS       DBNFEG010000058        11160 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.058, whole genome shotgun sequence.
ACCESSION   DBNFEG010000058 DBNFEG010000000
VERSION     DBNFEG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11160
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.058"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..293)
                     /locus_tag="AC1PNG_001038"
     CDS             complement(<1..293)
                     /locus_tag="AC1PNG_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HIP6701548.1"
                     /translation="MILFYEYPKCSTCRAAKAELKSLGLEFEAIDIKSTPPSAEELKA
                     WMEATGLELKKYFNTSGNSYRELGLKDKFDSLSLEQALNLLANDGMLIKRPLL"
     gene            complement(277..783)
                     /locus_tag="AC1PNG_001039"
     CDS             complement(277..783)
                     /locus_tag="AC1PNG_001039"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIP6701549.1"
                     /translation="MLYKQVYHSPLGPMSLIASDKGLRGAWFEGQKYFERGLNEKAIL
                     ASHPILDQTVRLLDQYFSGVQVDFSNLPLEAVGTNFQEHVWQLLKMIPIGQTTTYGQL
                     AQQLGLRSGQAVGGAVGRNPYSIIVPCHRVLNQKGQLTGYAGGLDKKIWLLRHENPEF
                     EVKDDFIL"
     gene            complement(786..1967)
                     /locus_tag="AC1PNG_001040"
     CDS             complement(786..1967)
                     /locus_tag="AC1PNG_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIP6701550.1"
                     /translation="MVFSVRTFNNINQIGLKELGNKFQIDGDHAANPDAFIIRSENLH
                     GFDFPENLKAIARAGAGTNNIPIEEATEKGIVVFNTPGANANAVKEAVIASILLSARD
                     YIGATAWANTLSGDDVPKQVEAGKKQFAGTEISGKTLGVIGLGAIGARIANDARRLGM
                     NVLGYDPYVSVETAWTISSHVKRVDDLKEIFTNADYITVHVPLTDKTRGLFNADSFGQ
                     MRKGTTLINFARGELVDNADLFEAIEAGVIKNYITDFGTEEVLNKDNIIVFPHVGGST
                     EEAELNCAIAAGQTIRRFMETGEIINSVNFPNVKQFLDAPYRITLINKNIPNMVATIT
                     TAVSELGINIDNIINKSKGDYAYTLLDLDEADKVKIEQLVAKFEATDAIVKVRVIQNK
                     N"
     gene            complement(2341..2898)
                     /locus_tag="AC1PNG_001041"
     CDS             complement(2341..2898)
                     /locus_tag="AC1PNG_001041"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6701551.1"
                     /translation="MEIRLAHPNEMEAIVDILEDARAFLATSGINQWQGEYPNKENIF
                     DDILSGRGYVGIVDGKVAAYAAVHRGQEAEYEAIYEGKWQHNHPVYTTFHRIAAADEF
                     RGQGIIQTFLQGLIEGQKGPDFRCDTHEQNKTMQHILKKLGYVYCGKVPIDGERLAYQ
                     KIKQKSERSLYQEISEDDRWLLGNN"
     gene            complement(2898..3989)
                     /gene="serC"
                     /locus_tag="AC1PNG_001042"
     CDS             complement(2898..3989)
                     /gene="serC"
                     /locus_tag="AC1PNG_001042"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIP6701552.1"
                     /translation="MTIYNFSAGPAVLPKPVLERAQAEFLDYNGSGMSVLEMSHRSKD
                     FDDIIKGAEATLRELMAIPDNYKVIFLQGGASLEFTMIPLNFAQGKKAYYLVGGSWGK
                     KAYTEAVKLSKTIAFEPILLGSTEDITYAELPTFDKNDIDPTAAYVHLTTNNTIEGTT
                     VYGIPDTNGVPVIADMSSNILAARYNVEDFAMIYAGAQKNIGPAGVTVVIVREDFLND
                     QPMLSSMLDYRIQAENDSLYNTPPAYSIYISKLVFEWVKEIGGVDEMEKINREKSGLL
                     YDYIDQSNFYTNPVRKKEERSVANIPFVSPSEELDAKFVKEATTAGFKNIKGHRSVGG
                     MRASLYNAFPHQGVVELIEFMKKFAAENA"
     gene            complement(4459..5322)
                     /gene="rsmI"
                     /locus_tag="AC1PNG_001043"
     CDS             complement(4459..5322)
                     /gene="rsmI"
                     /locus_tag="AC1PNG_001043"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIP6701553.1"
                     /translation="MKVQKSFKGQTSFGTLYLVPTPIGNLQDMTFRAVQILKEVDIIA
                     AEDTRNTGLLLKHFEVETKQISFHEHNAHEKIPVLIDWLKSGQSIAQVSDAGLPSISD
                     PGHDLVGAAIAEDIPVVALPGASAGITALIASGLAPQPHIFYGFLPRKSGQQKEFFQE
                     KKTYPETQIFYESPYRVADTLENMLTVYGDRQVTVVRELTKLYEEYQRGSISELLEYL
                     EENPLKGECLIIVEGAGEEVYPSADEVDLKAEVEREIASGIKPNQAIKEVAKRYQLKK
                     QEVYDIYHGLG"
     gene            complement(5324..5641)
                     /gene="yabA"
                     /locus_tag="AC1PNG_001044"
     CDS             complement(5324..5641)
                     /gene="yabA"
                     /locus_tag="AC1PNG_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HIP6701554.1"
                     /translation="MDKKEIFDALDDFSQNLLTTLAEVDAIKKHLQGVIDENTTLRLE
                     NSKLRERLEKEDKTGHKSSNFGKENLENIYEDGFHICTFSYGQRRDNDEPCMFCVELL
                     NRD"
     gene            complement(5661..6545)
                     /locus_tag="AC1PNG_001045"
     CDS             complement(5661..6545)
                     /locus_tag="AC1PNG_001045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HIP6701555.1"
                     /translation="MKIEELRNLQPNVFQRFVTILEQRRLVHAYLFSGDFASFDMAIF
                     LSQSLFCQEKEGVLPCQKCRSCRLIEANEFSDLTVIAPQGNVIKTDTIRELVKNFSQS
                     GFESNKQVFIIRDAEKMHANAANSLLKVIEEPQSAIHILLLTNQEEAVLPTIKSRTQI
                     IGFPKNIPAMERMLEEEGLLKTQANLLAQLVSSQEEAKKLAENKNFLDLMGQTKKFTD
                     LLLVEPTRAYLQVGILVSLAVEKAEQGRLFDLLSLILSEKMMESANVREKMDAVLKAK
                     KMWQANVSLQNSLEYITL"
     gene            complement(6542..7180)
                     /gene="tmk"
                     /locus_tag="AC1PNG_001046"
     CDS             complement(6542..7180)
                     /gene="tmk"
                     /locus_tag="AC1PNG_001046"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIP6701556.1"
                     /translation="MRNGFFITFEGPDGAGKTTVLQQLLPALQELGPEVVTTREPGGV
                     AIAEEIRNIILNPANTEMDDKTELLLFIAARRQHLKEKILPPLAQGKLLLIDRFIDSS
                     IAYQGFGRGLDIGDIHWLNQFATDGLKPDLTLYFDIDTEEGLARIARNADRDVDRLDM
                     EKADMHRRVRQGYLNILEQEPERFVKIDASQPLEAVVADALAVIKKRFAERT"
     gene            7355..8221
                     /locus_tag="AC1PNG_001047"
     CDS             7355..8221
                     /locus_tag="AC1PNG_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIP6701557.1"
                     /translation="MVRLKNILLILLGAGLFAFGLNYLIMPNRLFEGGATGLTLIIYY
                     LFHIQPWIMNIVINIPLFILGWKILGKKTLYLSILGTFSVTFWLAIFEKIHFSINLQQ
                     DLILVSILGGILMGLGLGTIFRAGGTTGGSDIIARIGHKFLPYSIGQIILAVDILILT
                     LIVIVFKDLRTVLYTLMMVAIASKVIDFVTEGGYGSKGVMIVSQKSDQLAQAIDCEIE
                     RGVTFIKAQGFYSKADVNMIYSVIYKSQLQEMKELIHRIDPHAFITITDAHEVLGEGF
                     TLDRDKKPIERH"
     gene            complement(8258..9529)
                     /locus_tag="AC1PNG_001048"
     CDS             complement(8258..9529)
                     /locus_tag="AC1PNG_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HIP6701558.1"
                     /translation="MSGFNFDIDQIANNSLSTKDKTTELIQATPTGDNSKVSFLAQLT
                     PEQQTGIRAKAPQLVDNFLANQNALLDFGKEAVEEVNATVNHILSEQKKIEIPQVDEL
                     LANTNRELNGFVAKYKDISTTAELEKKPGFFQRLFKQTKNDLQEFYFDSQTIEKKMDS
                     MAASVVKQEEVLARNIVSAELLIENNTKSIENLVGVIAFIEATGQEAAERAKLAQENL
                     ATLVPTTVDYQVASEKLARVTEVANILEQQHSEYMSRLYVAWTTTPQMRNLVKVSSDM
                     KQRLGMLRRNTIPTMKLSIAQLGILQQSIKSSQTADAIVNANNAALQMLADTSKEAIP
                     MMERTAQNPTLSVASVTKLAESLVAQNNGIIAAIDEGRQKRRELEAAIVRSAETINDS
                     VKLRDQKIIAALLEQGKEAQSEAEQPLETNQ"
     gene            complement(9535..10362)
                     /locus_tag="AC1PNG_001049"
     CDS             complement(9535..10362)
                     /locus_tag="AC1PNG_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701559.1"
                     /translation="MGHKYKKYKKYGKNNSGLVALLVLLAIGFVMSFWYILLPTGLLI
                     GYFYKREAIQRFLNRDSIKRSQVLALSIRSGYEKHQELFVEKGEDKVVLQLRNRLLGQ
                     LFELDQLYQKVGKYLNGYESKEVVDTLQLKYHLKLPEAKTEKNTDTQVSTGGNAVMDE
                     QGMIADLAPEILETYCNIQRDNLVILEKLEKATDKREELTAIHEANMNRFNDILQGYL
                     KIKKSPKDYFNAEERLVQAKSAMEMFDKDLDDTIKQFNEAGMKDFEVSLRMMKREEE"
     gene            complement(10456..11112)
                     /locus_tag="AC1PNG_001050"
     CDS             complement(10456..11112)
                     /locus_tag="AC1PNG_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIP6701560.1"
                     /translation="MSVKDFMTRKVVYISPDTTIAHAADIMREQDLHRLPVIENDKLV
                     GLVTEGTIAEASPSKATSLSIYEMNYLLNKTKVKDVMIKNVITVSGYASLEDAAYLMY
                     KNKVGILPVVDNGQLYGVITDRDIFAAFLHVSGYGEEGVRARFLVENKAGELEKIIRL
                     VADKDYNIVSTVQLATKSGKVVIEVQIDGKVDVEEVRTLFEGAGIQVDSLVPTVAKNI
                     "
BASE COUNT         3167 a         2504 c         1969 g         3520 t
ORIGIN      
        1 attagtaggg gacgcttaat cagcatacca tcattagcta gaagattaag tgcctgctcc
       61 aagctaagac tatcaaactt atctttcaaa cctagttcac gatagctatt cccagaagta
      121 ttaaaatact ttttcaattc tagtcctgtc gcctccatcc aagcttttag ctcttcagca
      181 ctaggcggtg ttgacttaat gtcaatagct tcaaattcca atcccaaact cttcagctcc
      241 gcctttgctg ctcggcaagt cgaacatttt ggatactcat aaaataaaat catctttcac
      301 ctcaaattct ggattctcat gtcgcaagag ccagattttc ttatccagtc cacctgcata
      361 acctgtcaac tgtcctttct gattgagaac tcggtgacaa ggaacaataa tcgaataagg
      421 attacgccca actgctcctc ctactgcctg accagaacga aggcccagtt gctgagccag
      481 ttgtccatag gtagtcgtct gaccaatcgg aatcatcttc aataattgcc agacatgctc
      541 ttggaaattt gttcccacag cttctaatgg aaggtttgaa aagtccacct gaacccctga
      601 aaaatactgg tcaagcaatc gaactgtctg gtctaaaatg ggatgggagg ctaaaatagc
      661 tttctcattc aagcctcgtt caaaatattt ttgtccctca aaccaggccc ctctcaatcc
      721 cttatcacta gcaattaatg acatggggcc aagtggactg tgatagactt gtttatacag
      781 catgattagt ttttattctg aatgacacgc actttgacaa ttgcatccgt tgcttcaaat
      841 ttagcaacca actgctcaat tttcacttta tccgcttcat ccaaatcaag taaagtatag
      901 gcatagtcac ctttagactt attgatgata ttatcgatat tgattcccaa ctcagataca
      961 gccgttgtga tcgttgccac catattagga atattcttat taatcaaggt aatacgatac
     1021 ggtgcatcta ggaattgttt cacatttggg aagttaactg agttgataat ttcacccgtc
     1081 tccataaaac gacgaatggt ctgaccagca gcaatggcac agttgagctc tgcttcctct
     1141 gttgacccac caacgtgcgg gaagacgata atgttgtcct tgttgagcac ttcttctgtc
     1201 ccaaaatccg tgatatagtt cttgataact cctgcctcaa ttgcttcaaa caaatcagca
     1261 ttatcaacca attcacctcg ggcaaagttt atcaaggttg ttccttttct catttgacca
     1321 aagctatccg cattgaagag tcctcgtgtc ttatctgtca aaggaacatg aacagtaatg
     1381 taatccgcat tggtaaagat ttctttcaag tcatccacac gtttgacatg gcttgaaatg
     1441 gtccatgcag tttctactga tacataaggg tcgtaaccga gaacattcat ccccaaacgg
     1501 cgggcatcgt tggcaatacg cgcaccgata gcaccaagtc cgataacacc aagagtctta
     1561 cctgaaattt ctgtcccagc aaactgcttc ttacctgctt caacctgctt aggtacatca
     1621 tctcctgaca atgtatttgc ccatgcagtc gctccgatat agtcacgtgc tgacaaaaga
     1681 atggaagcaa tgactgcctc ttttacggca ttggcattgg cacctggagt gttaaagaca
     1741 acaatacctt tctctgttgc ctcttcgatt ggaatattgt tagtccctgc accagcacgg
     1801 gcaatagctt ttagattttc aggaaaatca aatccatgca aattttcaga tcgaataata
     1861 aaggcatctg gatttgctgc atgatctccg tcaatctgga atttattgcc taattcctta
     1921 agaccaattt gattgatatt gttaaaagta cgtacgctaa ataccattta tttttaccta
     1981 aaaaggggag tgggaaagaa ctcaaccact cctagaagag ttcgtcaaca agtctttatt
     2041 tctatttgtt gagctgaaac agtctattcc cagactgttt cactcccacc cccgcacagc
     2101 tcaaactgtc tgggagacag tttgaggttg gagataaggt gaactctgtt cgcatcagtc
     2161 gtagaggtca gatttggagt ataaaatacg aactgctgag atttgcttcg caaatcttat
     2221 ctccaacctt taacagtcca ctggactgtt aaacccaatc aaccactgtg ctgagatgtt
     2281 gactcgaacc ttgagaagtg atatgaagct ttttcccggt ccttttcttt ctttatttca
     2341 ttaattattt ccgagtaacc aacggtcgtc ttcactgatt tcctggtaaa gactccgctc
     2401 gctcttttgc ttgatttttt gataggctag acgttctcca tctattggga ctttaccaca
     2461 atagacatag cctaattttt ttagaatgtg ctgcattgtc ttattctgct cgtgggtatc
     2521 acagcggaaa tccggtcctt tttgaccttc aattaaacct tgcaaaaaag tttgtatgat
     2581 tccttgacca cgaaattcgt cagctgctgc aattctatga aatgttgtat atacaggatg
     2641 attgtgctgc cattttcctt catatatggc ctcatattct gcttcttgac cacgatgaac
     2701 ggcggcatag gctgccacct taccgtcaac tataccaacg taacctctgc cagaaagaat
     2761 gtcatcaaag atattctctt tattaggata ctctccctgc cattgattga taccagaggt
     2821 tgctagaaat gcacgagcat cttcaagaat atcgacaata gcctccatct catttggatg
     2881 tgctaatcta atttccatta agcattctcc gctgcaaatt ttttcatgaa ttcaatcaat
     2941 tcaaccacac cttgatgtgg gaaagcatta tagagcgacg cccgcatccc acctactgaa
     3001 cggtgacctt tgatgttttt aaaaccagct gttgttgctt ctttgacaaa cttggcatct
     3061 aattcttcac ttggtgagac aaaaggaatg ttcgctactg aacgctcttc ttttttgcga
     3121 acagggtttg tatagaaatt agactggtcg atatagtcat aaagcaaacc agatttttca
     3181 cggttgattt tttccatctc atcaacacca ccaatttctt ttacccattc aaagacgagt
     3241 ttagaaatat agatagaata agctggtggc gtattataaa gagagtcatt ttctgcttga
     3301 atacggtagt ccaacatgct tgaaagcatt ggttgatcat tgaggaagtc ctcacgaaca
     3361 atcaccaccg tcacaccagc aggaccaata tttttctgag cgccagcata aatcatggca
     3421 aaatcttcaa cattatagcg agctgctaag atgtttgaag acatgtcggc aatgactggc
     3481 acaccattgg tgtcaggaat accataaact gttgttcctt caatagtgtt atttgttgtc
     3541 aaatgcacgt aagcagcagt tggatcaata tcatttttat cgaaggttgg caattctgca
     3601 taggtaatat cttccgttga tccaagcaaa attggttcaa aggcaattgt tttagacagc
     3661 ttcacagcct cagtataggc ttttttaccc catgagccac ctacaagata gtaggctttc
     3721 ttaccttgag cgaagttgag aggaatcatt gtgaattcca aagaagctcc accttgcaag
     3781 aaaataacct tgtaattatc tggaatggcc atcaattcac gaagcgttgc ctcagcacct
     3841 ttaataatat catcgaagtc cttggagcga tgcgacatct ccaaaacgct cattcccgaa
     3901 ccattgtagt ccaagaattc agcttgggcg cgttcaagta ctggttttgg caatactgca
     3961 ggacctgcag agaagttgta gattgtcata aaatccctcc attaagttag tctgttcatt
     4021 atatcacaaa tattcagaca attccataaa atatgattat tttgtaaaaa cattcggatt
     4081 tcttatattt attctacttt ctaaagaacc tcttactttt agccataaac gaggcgcttg
     4141 cctcacaggg attcatcagg tttcaatgaa aattttatga ccagatttga ctgtttgggg
     4201 agtgtcgcca attgctatct gcatattaat actcaatgaa aatcaaaagt agcttagctg
     4261 ggatatagtt gaaggctgga agtatagaga actgtgttcg cgtcattaag ccgaagatag
     4321 aactctgttc ctttctcatt tcaaggtaac agactgaaaa gatccactgg agcttttcac
     4381 tcatcaaggc tcttgacaac ggataatttt gattttcgaa gagtataagc ttgcataaac
     4441 atacaaaaaa actaggtctt aacctagtcc atgatatata tcgtatactt cttgtttttt
     4501 gagttggtag cgtttggcca cttctttgat agcctgatta ggttttatgc cacttgcaat
     4561 ttccctttct acctcagcct tcaagtctac ttcgtcagca gaaggataga cttcctcacc
     4621 agcaccctcg acaataatca agcattctcc cttaagtgga ttttcttcca aatactctag
     4681 taattcggaa atgcttcctc tctggtattc ttcgtataac ttggtcaatt ctcgaacaac
     4741 tgttacctga cgatcaccat aaactgtcaa catattttct aaggtatctg ccacacgata
     4801 tggagattca tagaaaattt gcgtctcagg ataggtcttt ttttcttgga aaaattcttt
     4861 ttgctgcccc gattttctcg gtaaaaatcc ataaaaaata tgtggctggg gtgccaaacc
     4921 agatgcaatc aaggctgtaa ttcctgccga tgctcctgga agtgctacaa caggaatatc
     4981 ttccgcaatc gctgccccta ccaggtcatg accaggatcc gaaatggaag gcaggcctgc
     5041 atcagaaact tgcgcaatgg actgaccaga tttgagccaa tcaatcaaaa ctggaatttt
     5101 ttcatgggca ttgtgttcat gaaaggatat ttgcttggtt tctacttcaa aatgtttgag
     5161 taggagtcct gtatttcttg tatcttccgc tgcaattata tccacttctt tcaaaatctg
     5221 aacagctcgg aatgtcatat cctgtaagtt tccaataggc gttggcacca gatataaagt
     5281 cccaaaactc gtctgtccct taaatgattt ttgtactttc atcctaatct cgattcaata
     5341 attctacaca gaacatacaa ggttcatcat tatccctacg ttgaccataa gagaaggtac
     5401 aaatatggaa accatcttcg taaatattct ccaaattctc cttaccaaag ttggaagatt
     5461 tatggcctgt cttatcttct ttttccaaac gttcgcgcaa cttggaattt tccaagcgga
     5521 gggtcgtatt ttcatcaata actccctgta aatgcttttt gatagcatcc acttctgcca
     5581 acgtggttag aagattctgc gaaaaatcat ctagggcatc aaaaatttct tttttatcca
     5641 ttatctccct ttcttttatt ttacagagtt atatactcta gactattttg tagacttacg
     5701 tttgcctgcc acatcttctt agccttaaga acagcatcca ttttttcacg tacgtttgcc
     5761 gattccatca ttttctcaga taatatcaga ctcagaaggt caaacaaacg tccctgctca
     5821 gctttttcca cagctaaaga cactaggatt ccaacttgca aataagcacg tgtaggctcc
     5881 accaagagca agtctgtaaa tttctttgtc tgtcccatca aatctaaaaa gtttttattt
     5941 tctgctaact ttttcgcttc ttcttgactt gaaaccagtt gagctaagag attcgcttgt
     6001 gtttttaaca agccctcctc ttctaacatt cgctccatgg ccggaatatt tttaggaaaa
     6061 cctataatct gtgtccgact cttgatagta ggaaggacag cttcttcctg attggtcaat
     6121 aataagatgt gaatagctga ctgaggttct tcaatcactt ttagcaaaga gttggctgcg
     6181 ttagcatgca ttttctcagc atcacgaata atgaaaacct gcttattgga ttcaaatcca
     6241 gattgtgaaa aattcttaac taattcacgt atcgtatctg ttttgataac atttccctgc
     6301 ggtgcaataa cagtaagatc tgaaaactca tttgcttcaa ttagacgaca agaccgacac
     6361 ttctgacaag gaaggacgcc ttctttctcc tgacaaaata acgattgact gagaaaaatt
     6421 gccatatcaa aactagcaaa gtcacctgaa aatagatagg catgcaccaa acgtctctgc
     6481 tctaaaattg tcacaaaacg ttgaaataca ttgggttgta agtttctaag ttcttcaatc
     6541 ttcatgttct ttctgcaaac cgtttcttaa tgaccgccag agcatctgca acaacagcct
     6601 ctagtggctg actggcatca atctttacaa accgctctgg ttcctgctct aaaatattaa
     6661 gatagccttg acgcacccgt ctatgcatat ctgctttttc catatccaag cgatccacat
     6721 cacggtcagc attgcgggca attcgagcca gcccctcttc tgtatcaata tcaaaataga
     6781 gggttaaatc tggctttaaa ccatctgttg caaactggtt gagccaatga atgtcaccta
     6841 tatccaaccc ccgtccaaat ccttgatagg caatagagga atcgataaaa cgatcaatca
     6901 agagcaattt cccctgtgcc aagggtggaa gaatcttctc tttcaaatgc tgacgtcgtg
     6961 ctgcaatgaa caataacagc tccgtcttat catccatttc agtatttgca gggttcagaa
     7021 taatattgcg aatttcttct gcaatagcca ccccacctgg ctctctagtt gtgactactt
     7081 ctggccccaa ttcctgcaag gctggcaata actgttgcaa taccgtcgtt ttccctgccc
     7141 catctggacc ttcaaatgta ataaaaaaac cgtttctcat ctttccctcg ctcttagtac
     7201 cagtatgcac agttcagcac ttcacttgtg aagactggtt cttagatatt cattccattt
     7261 taccaaaaat tagtcaaaaa atcacttgaa aattgacttg tttttaagga gaaaatcttt
     7321 taaaattgat atatacacat attcggagat gcttatggtt cgtttgaaaa atattttact
     7381 tattttgctt ggtgcagggc tatttgcctt tgggctaaat tatttaatca tgcccaatcg
     7441 tctgttcgag ggtggcgcaa ctggattgac gctgattatt tactaccttt tccatattca
     7501 accttggata atgaacattg tgattaatat tccattgttc atcctcgggt ggaaaatact
     7561 cggaaagaag actctctatc tcagtatctt aggaaccttt agcgttacct tttggttggc
     7621 catttttgaa aaaatccatt tttcaatcaa tctacaacaa gatttgatac tcgttagtat
     7681 cctgggtggg attcttatgg gactaggatt aggaaccatc ttccgtgctg gcggaacgac
     7741 tgggggaagc gatatcattg ctcgtatagg tcataaattc ctaccttact ccattggaca
     7801 aatcattctt gcagttgaca tcctcatcct aactctgatt gttattgttt ttaaagacct
     7861 ccgaacagtt ctatatactt taatgatggt tgctattgct tccaaggtta ttgattttgt
     7921 cactgagggc ggatatggta gcaagggggt tatgattgtt tctcaaaaat ctgaccaatt
     7981 ggctcaggct attgattgtg aaatcgagcg tggtgtcacc tttatcaaag cacaaggttt
     8041 ctacagcaaa gccgatgtca acatgattta ctcggtcata tataagagcc aactccaaga
     8101 gatgaaggag cttattcatc gtattgaccc ccacgcattt atcaccatta cagacgcaca
     8161 tgaagtactc ggtgagggat ttactttaga tcgagataaa aaaccaatag aacgccatta
     8221 aacaaacctg cagtttcgct gaactgcagg ttttatatta ttgattggtt tcaagtggtt
     8281 gctctgcctc agactgtgct tctttgcctt gctccagcaa agcagcaatg attttttgat
     8341 cgcgtagctt aacagaatcg ttgatagtct ctgctgaccg tacaatagct gcctccaact
     8401 cacgacgttt ctgacgccct tcatctatag ctgcaataat gccattattt tgggcaacca
     8461 aactttctgc caatttggta acagatgcta ctgaaagggt cggattctga gctgtgcgtt
     8521 ccatcatcgg gatggcttct tttgatgtat ccgcaagcat ttgaagggca gcattattag
     8581 cattgacaat cgcatctgcc gtctggctag acttgataga ttgttgtaag atgcccaact
     8641 gtgcaatgga caatttcatt gttggaatag tattgcgacg taacatccca aggcgctgtt
     8701 tcatatccga agaaaccttg accagattac gcatctgcgg tgtcgttgtc cacgcaacat
     8761 acaagcgact catatactca gaatgttgtt gttcgaggat attagcaacc tctgttacac
     8821 gtgccaactt ctcagaagct acttgataat cgactgtagt cggtactaaa gttgcgaggt
     8881 tttcttgtgc caattttgca cgctctgccg cttcctgacc agtcgcttca ataaaggcga
     8941 tgacccccac caaattctcg atagacttgg tattattttc aatcaaaagc tctgcagaaa
     9001 caatattgcg cgccaacacc tcttcctgct tgaccacact ggctgccatg ctatccattt
     9061 tcttttcaat ggtctgcgag tcgaaataaa attcctgcaa atcatttttg gtctgcttga
     9121 acaggcgttg gaagaaacct ggtttctttt ctaattcagc agttgtggaa atgtccttat
     9181 acttagccac aaaaccattc aattcacggt tggtattggc caaaagctca tccacctgtg
     9241 gaatttcaat cttcttctgt tctgacaaaa tgtgattgac cgtggcattg acttcttcta
     9301 ccgcttcctt accaaaatcc aagagggcat tttgattagc taagaaatta tccaccaatt
     9361 ggggagcctt agcacgaata cctgtttgtt gttccggtgt caactgtgcc aagaaagaca
     9421 cttttgaatt atctccagtc ggagtcgctt gaataagctc tgttgtctta tctttggttg
     9481 ataaactatt attagcaatc tgatcaatat cgaaattaaa tcctgacatc ttctctattc
     9541 ctcttctctt ttcatcatac gcagactgac ttcaaaatct ttcatacccg cttcgttaaa
     9601 ttgtttgatg gtatcatcca aatccttatc aaacatttcc atggctgatt ttgcctgcac
     9661 caatcgttct tctgcgttaa aataatcttt cggtgatttt ttgattttca aataaccttg
     9721 taaaatatcg ttaaaccgat tcatattggc ctcatggata gctgtcaatt cttccctctt
     9781 atctgtcgcc ttttccagct tttcaagaat aacaagattg tctctttgaa tattacaata
     9841 ggtttctaga atttcaggag ctaaatccgc tatcatgcct tgttcatcca tcacagcatt
     9901 accaccagta gaaacctgtg tgtcagtatt tttttctgtt ttagcttctg gcagttttaa
     9961 atgatatttt agttgcaagg tatctacaac ttctttactt tcatatccat tcagatattt
    10021 tccaactttt tgatagagct gatctaattc aaataattgt cctaacagtc tatttctcaa
    10081 ctgtaacaca accttatcct cacccttttc cacaaagagc tcttgatgct tttcatagcc
    10141 cgaacgaata gatagcgcta atacctgcga gcgttttata gaatctctat tcaaaaaacg
    10201 ctggatagcc tctctcttgt aaaaatagcc aattaagagc ccagtaggca ataagatata
    10261 ccagaaactc atgacaaaac cgattgccaa taaaacaagg agggccacaa gtcctgaatt
    10321 atttttacca tatttcttat actttttata tttgtgcccc atagagcctc ctcatacgtg
    10381 atatacttta ttatatcaca ttcccctaaa atactagaat gatagctaaa gaaaaaaacc
    10441 gagaaatctc ggtttttaaa tatttttagc aacagtcgga accaagctat ccacttggat
    10501 acctgcacct tcgaaaagag ttcgaacttc ttcaacatca acttttccgt caatctgcac
    10561 ttcgatgaca acttttccag acttggtagc aagttgcaca gtagatacaa tattataatc
    10621 cttatctgcc accaatcgaa taattttttc taactcccct gccttatttt caacaaggaa
    10681 ccgagcgcgt actccttctt ctccataacc tgagacatga agaaaagctg caaaaatatc
    10741 acgatcagta atgaccccat atagttggcc gttgtcaaca actggaagaa tccccacttt
    10801 gtttttatac atgagataag cagcatcttc aagactagca taacctgaga ctgtgataac
    10861 attcttaatc atcacatcct tgaccttagt cttattcaaa aggtagttca tctcgtaaat
    10921 agataggcta gttgccttcg atggactagc ttctgcgatt gttccctcag tgaccaaacc
    10981 aaccaactta tcattttcaa taacaggtag acggtgcaaa tcctgttcac gcataatatc
    11041 tgcagcgtgt gcaatggttg tatctggtga aatataaaca actttacggg tcataaaatc
    11101 tttaacagac ataggacttc ctcctttggt aaatcatact ttattatatc acaaatccga
//
LOCUS       DBNFEG010000059        11095 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.059, whole genome shotgun sequence.
ACCESSION   DBNFEG010000059 DBNFEG010000000
VERSION     DBNFEG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11095
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.059"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            64..1101
                     /locus_tag="AC1PNG_001051"
     CDS             64..1101
                     /locus_tag="AC1PNG_001051"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIP6701561.1"
                     /translation="MKEGDLLFTPISEKIDINQVREHSKLSPETLAKKQARDRELEAI
                     LKGEDNRLLLVIGPCSSDNEEAVLDYAHRLAKLQEEVKDKIFMVMRVYTAKPRTNGDG
                     YKGLMHQPDTDAAPSLINGIKAVRNLHYRVITETGLTTADEMLYPENLPLVDDLVSYI
                     AIGARSVENQQHRFVASGIDVPTGLKNPTSGNLKVMFNGLFAAQKKQSFLFNNTEVET
                     SGNPLTHVILRGAVNENGKYLPNYYYDNLLETIDLYDQFGLKNPFIIIDTNHDNSGKN
                     YLEQIRIVRQTLINRDWNESIRNYVRGFMIESYIEDGRQDNPDVYGKSITDPCLGWEK
                     TQELIREIYNR"
     gene            1111..2136
                     /locus_tag="AC1PNG_001052"
     CDS             1111..2136
                     /locus_tag="AC1PNG_001052"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIP6701562.1"
                     /translation="MGIHKRSTSLDIDKVKELSKLEGEFLAAKNRRDEELKRIIRGED
                     DRLLLVIGPCSSDNEEAVLEYARRLSKLQEEVKDKIFMVMRVYTAKPRTNGEGYKGLV
                     HQPDTSKLPDLINGIAAVRNLHYRVITETGLTTADEMLYSANYPLVEDLVSYHAIGAR
                     SVEDQEHRFVASGVDMPTGMKNPTSGNLTVMFNGIYAAQNKQNFIYRDAEVDTDGNPL
                     AHAILRGANTEKGGYEPNYYYDILLKTIKQYEQFGLKNPFIVIDTNHDNSGKTYLEQI
                     RIVRQTLINRAWNESIRKYVRGFMIESYLEDGRQDSPEVFGKSITDPCLGWKKTEALI
                     REIHQTV"
     gene            2182..3051
                     /locus_tag="AC1PNG_001053"
     CDS             2182..3051
                     /locus_tag="AC1PNG_001053"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIP6701563.1"
                     /translation="MNIDGYTRLAAVVAKPIKHSISPFIHNLAFKETGVNGVYVAWEI
                     PEEDLAVTLENIKRYDMFGINLSMPYKQAVIPYLDDLTASARLIGAVNTVIHKDGKLI
                     GHNTDGIGFFKSLERLKGFQVKNKRLTILGGGGASTAIIAQATLDGAKEITIFCRQQS
                     LERTQASLTPIAQATGVPMQVLALDDSQLLQEHITNSDLLVNGTSVGMDGYSQPVPST
                     IRFPENLLVADVIYQPFETPLLKLAQSQGNPTINGLGMLLFQAAEAFQAWTGKEMPTD
                     LIWDQLVQKYDIK"
     gene            3064..4131
                     /gene="aroB"
                     /locus_tag="AC1PNG_001054"
     CDS             3064..4131
                     /gene="aroB"
                     /locus_tag="AC1PNG_001054"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIP6701564.1"
                     /translation="MKLTVNLPNTPYDILIQRGSLAQTGTWIKELWKPQKIAIITDDH
                     VGSLYRETVQSSLEQAGFETIVFEFPEGEASKNLDTVNQAYEFLVKNGMTRSDGIIAL
                     GGGVVGDLAGFVASTYMRGIHFLQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFCQP
                     DGVLIDPDTLKTLGKRELIEGMGEVVKYGLIDDVELWETLDQLDGSVESILEHADYII
                     YHSCEVKRKVVVEDELDNGVRLYLNFGHTIGHAIEVTAGYGQVMHGEAVAIGMVQISR
                     AAEKKGLMPAGMTEKIIAMCEKFGLPTTHQPWNVDELYATLTHDKKARGKTIKLVIVP
                     QLGQAAIHQIPMEEMLEFLQV"
     gene            complement(4335..5135)
                     /locus_tag="AC1PNG_001055"
     CDS             complement(4335..5135)
                     /locus_tag="AC1PNG_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6701565.1"
                     /translation="MSTILSIQNIHKTFEAGTVNENHVLRGLNLDVKEGDFISIIGGN
                     GAGKSTFMNSLAGALTVDSGDILLEGKSIKHVSAAKRSKDISRVFQDPKMGTASRLTI
                     EENMAIAYRRGLSRGLGWGVKDSERAILKEALKELGLGLENRMKVDTQFLSGGQRQAL
                     TLMMASLVKPKVLLLDEHTAALDPKNSDMVMELTKKIVESHQLTALMITHNMENAIEY
                     GNRLVMLHRGQIVVDVEGEEKKNLTVQNLMDLFYKNSGEKLTDDEMIL"
     gene            complement(5145..6038)
                     /locus_tag="AC1PNG_001056"
     CDS             complement(5145..6038)
                     /locus_tag="AC1PNG_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6701566.1"
                     /translation="MDIILSSISQGLLWSVMAIGVYLTFRILDIADMTAEGSYPLGAA
                     VCATGIVNGVNPLLATFMAMVAGMGAGLISGLLHTKLKIPALLTGIVTLTGLYSINLK
                     ILGKANVALLKQETLVTQLQDFGLTKTNAVLVIGLVFVLAVVGLLTLLLNTQIGLAIR
                     STGDNIPMSEANGINVDNMKIYGYMLSNGLIALCGALLTQNNGYADLNSGTGTIVIGL
                     ASVIIAEVILRNLRLGWRLLSVVLGAVVYRLIILAILEIPSMDADLVKLFSAILLATV
                     LYVPELQKKLNIRRPKLNGNA"
     gene            complement(6093..7067)
                     /locus_tag="AC1PNG_001057"
     CDS             complement(6093..7067)
                     /locus_tag="AC1PNG_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HIP6701567.1"
                     /translation="MYYKLVKKLATISVASMGLLTLAACSSSSEQASSDVVKVGVLQY
                     MEHESLTAAREGFVAELEANGYKEGEKLVLDYQNAQGDQANLQTISEQLIDGNDIVLA
                     IATPSAQSLATVSNETPIVFTAVTDPLSAGLVESIEKPGGLLTGTSDQAPIDKQVELL
                     GQAVPDAKTVGILYTTSERNSEVQVEQAKELLEKAGYKVVVKGITSSNEVQDATTSLM
                     KDVDALFIPTDNTVASTMTMIGELSVEHKVPVIGGSTDMVDEGGLLTYGTNYEALGRQ
                     TAKMAIKIIEGANVSETAVEYPETVSLHVNEEMAQKLGIDTSKLAVSE"
     gene            complement(7091..8050)
                     /locus_tag="AC1PNG_001058"
     CDS             complement(7091..8050)
                     /locus_tag="AC1PNG_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP6701568.1"
                     /translation="MGFKKIILSSVALLSAVTLAACSSNSSDSSSVNVGIIQYAEHEA
                     LSSAREGFVEVLEEAGYKEGENLTIDLQNAQGDQANLQTMVEKLAGKNDLNFAIATPA
                     AQAMLNADSETSSVFTAVTDPVEAGLVESLEKPGGTMTGSTDATDVEGQIEMLLKVVP
                     TAKTVGIFYNSSEVNSEVQAEQAKKALEAKGVKVEVTTVTTTNDVQQAITSLAGKVDA
                     IYLPTDNTVASTASTIGDILKEAKVPAMGSDAAVIDAALFTYGVDYHAIGVQAGELAV
                     KILKGEKPADLAVEKPNTAAITVNEEMAKAVGIDAATIEALEE"
     gene            complement(8791..9792)
                     /gene="lacD"
                     /locus_tag="AC1PNG_001059"
     CDS             complement(8791..9792)
                     /gene="lacD"
                     /locus_tag="AC1PNG_001059"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HIP6701569.1"
                     /translation="MTKLQLSQAKFNHMTRLSNSDQVIAALAIDQRGALKRLLAAAAG
                     GGEFGDDILIDFKKVISSDLTPYASSILLDAEYGVPASELRHADCGFIAAYEKTGYDA
                     STPGRLPDLLPNWSAKRIKELGADAVKILLYYDVDDKPEINDIKHAWVERIGSECIAE
                     DIPYFVEILTYDASDMDVTSREYAALKPHKVNGAMREFSKSRYNADVLKVEVPVNMNF
                     VEGYTDEEPVYTVEEAKAFFKEQTDSTHLPFIYLSAGVSAKLFQETLVFAKEAGSSFN
                     GVLCGRATWKDAVAIFASEGEEAAKAWLADQGRRNVEELNEVLATTACPWTEKVEVK"
     gene            complement(9816..10961)
                     /gene="nagA"
                     /locus_tag="AC1PNG_001060"
     CDS             complement(9816..10961)
                     /gene="nagA"
                     /locus_tag="AC1PNG_001060"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIP6701570.1"
                     /translation="MTVFIKAKSIILPESEVESAYLEITDKGQFGRIVTEKPEGEIVD
                     YSDFHLAPGLVDTHIHGYASHDVMDNDFEGIKVISEGLLSCGVTSWLPTTLTDTTENL
                     DAVCETIGTYAGQETGAKIQGIFLEGPFFTEKYKGAQNPKYMSDPSIEKLDNWNSLSK
                     GLVNKIAIAPEREGVKEFIEFANSKAIHTALAHSDATYTQAEAAVEAGANIFVHVYNG
                     MSGLHHREPGMVGAALNLKNVYAEMICDGHHVHPVAAEIVVKARGAEETVLITDCMRA
                     GGMGEGESRLGEFEVVVKDGTARLKESGSLAGSILELIEAVQNVVKWGLVSLPDALRM
                     ASLAPARSVNIDHICGRIAEGRAADFIVVDDAGRLQATYLDGVKRFG"
BASE COUNT         3303 a         2525 c         2096 g         3171 t
ORIGIN      
        1 cataaaatca tggatattga ttttaaattc ttaaaaataa tggtataatt gaggaaccta
       61 taaatgaaag agggagatct attgtttaca ccaatcagcg aaaaaattga catcaatcaa
      121 gtacgagaac attcaaaact ttctccagaa acacttgcta aaaaacaagc tcgtgaccgt
      181 gaacttgaag ccatcttaaa aggtgaagat aatcgtcttc tcctagtaat tggaccatgt
      241 tcttctgaca atgaagaggc tgtcttagat tatgctcacc gcttggccaa gctccaagaa
      301 gaggtcaaag ataaaatttt tatggtcatg cgcgtctata ctgccaagcc acgtaccaat
      361 ggtgatggtt ataaaggact catgcaccaa ccagatacag atgctgcacc aagcctcatc
      421 aacggaatca aagcagttcg taacctccac tatcgtgtca ttacggaaac aggtttaact
      481 actgctgacg agatgctcta ccctgaaaac ctgcctttgg ttgatgactt ggtttcctac
      541 atcgctatcg gagctcgttc ggttgaaaat cagcagcacc gttttgtagc gtcaggcatc
      601 gatgttccaa caggcttgaa aaatccaaca tctggtaacc tcaaagtcat gttcaacggt
      661 ctttttgctg ctcagaagaa gcaatccttc ctcttcaaca atactgaagt tgaaacatct
      721 ggcaacccac ttactcacgt tattcttcgt ggcgcagtca atgaaaatgg taaataccta
      781 ccaaactatt actatgacaa tcttctagaa accattgacc tctatgatca atttggtttg
      841 aaaaatccat ttattattat cgataccaac catgacaatt ctgggaaaaa ttatttggaa
      901 caaatccgta tcgtccgtca aaccttaatc aaccgtgact ggaacgaatc cattcgtaac
      961 tacgttcgcg gattcatgat tgaatcctac attgaagacg gtcgccaaga caatccagat
     1021 gtctatggaa aatccatcac agacccttgc ttaggctggg aaaaaacaca agaactcatc
     1081 cgagaaattt acaacagata ggaagatatt atgggaattc acaaacgtag caccagttta
     1141 gacatcgata aagtaaagga actttccaag ttagaaggag aatttctagc tgcaaaaaat
     1201 cggcgcgatg aagaactgaa aagaattatc cgaggcgaag atgatcgtct gctcttggtt
     1261 atcggtcctt gctcatcaga taatgaagag gctgttctag aatatgcacg tcgtctctct
     1321 aagcttcaag aggaagtcaa agataaaatc ttcatggtca tgcgggttta cacagctaag
     1381 ccacgtacca atggggaagg ctataaagga ctggttcatc agccagatac ttctaaatta
     1441 ccagacctca ttaacggaat tgcagccgtt cgtaatttgc attaccgtgt cattactgag
     1501 accggactca caactgctga tgaaatgctc tactctgcca actatccact ggtggaagac
     1561 ttggtgtcct accatgctat cggagcacgt tcggttgaag accaagaaca tagatttgtt
     1621 gcatcaggtg tcgatatgcc aactggtatg aaaaacccaa cttctggtaa tttgaccgtt
     1681 atgttcaatg gtatctacgc cgcacaaaac aaacagaact ttatttaccg tgatgcagaa
     1741 gttgatacag atggcaaccc tctagctcat gctatccttc gtggtgccaa tactgaaaag
     1801 ggcggttatg agccaaacta ctactatgat attctcttga aaaccatcaa acagtatgaa
     1861 caatttggtc ttaaaaatcc atttatcgta attgatacca accacgacaa ttctggtaaa
     1921 acttacctgg aacaaatccg tatcgtccgt caaaccctca tcaaccgagc ttggaacgaa
     1981 tctattcgca aatatgtccg tggtttcatg attgaatcct acttagaaga cggccgtcaa
     2041 gatagccctg aggtattcgg caaatccatt accgaccctt gcttaggttg gaaaaaaacg
     2101 gaagctctga ttcgtgaaat tcatcaaacc gtctaattct ttccttaatt ttttatacga
     2161 taaataattt ggaggtaagc tatgaacatt gatggttaca ctcgtcttgc tgccgttgtt
     2221 gcaaagccaa tcaaacactc catctccccc ttcattcata atctggcttt taaggaaaca
     2281 ggtgtaaatg gtgtctatgt cgcttgggaa attcccgagg aagatttggc tgtaacgctt
     2341 gaaaatatca aacgatatga tatgtttggt atcaaccttt ccatgcccta taaacaagct
     2401 gtcattcctt atctagatga tttaacagcc tctgcacgcc tgattggcgc agtcaatacc
     2461 gtgattcata aagatggaaa actaatcggt cacaacaccg atggaattgg atttttcaag
     2521 agtttggaaa gactaaaagg ctttcaagtt aagaacaagc gactaaccat cctaggtggt
     2581 ggtggtgctt ctactgctat catagctcag gctacattgg atggtgcaaa ggaaattacc
     2641 attttctgcc gtcaacaaag cttggaaaga acacaggcga gtcttacacc tattgctcaa
     2701 gcaactggag ttcccatgca ggtcctagcg cttgatgata gccaattact acaggaacat
     2761 atcaccaact ccgacttact agtcaacgga accagtgtag gcatggatgg gtattctcaa
     2821 cctgttcctt ctaccattcg ctttcccgaa aatcttttgg ttgcggatgt gatttatcaa
     2881 ccattcgaaa cacctttatt aaaactagcc caatcacaag gcaaccctac tattaacggt
     2941 ttgggcatgc tccttttcca agcggctgaa gcattccaag cctggactgg taaggaaatg
     3001 ccgactgact tgatttggga ccaattagtc cagaaatacg acatcaaata gagaggaaat
     3061 ctgatgaaac tgaccgtcaa tcttcccaac actccctacg acatccttat ccaaagagga
     3121 agtttagcac agacaggtac atggatcaaa gaactttgga aacctcagaa aatcgccatt
     3181 attacagatg accacgttgg ctcactctat cgcgaaacag ttcagtcaag cttagaacaa
     3241 gctggttttg aaaccattgt ttttgaattt ccagaaggtg aagcctcaaa aaatcttgat
     3301 accgtcaacc aggcctacga atttctcgta aaaaatggta tgacacgaag tgatggaatc
     3361 attgctctcg gcgggggagt cgttggcgat ttggcaggtt ttgtcgcttc tacctatatg
     3421 cgaggtatcc atttcctaca aatcccaacc agcctaactg cccaagtcga ttcttctatc
     3481 gggggaaaaa ctggcgtgaa caccccattt gccaaaaata tggtggggac cttctgccag
     3541 ccagacggcg ttctcattga ccctgatacc ctaaaaacac tcggcaaacg agaattgatt
     3601 gaaggaatgg gcgaggttgt caaatatggc ttaatagatg atgtggagct ctgggaaacc
     3661 ctagaccaat tagacggctc tgtcgagagc attctggagc atgctgacta tatcatctac
     3721 cactcctgtg aagtcaaacg caaggttgtt gtcgaagatg aattagacaa cggtgtcaga
     3781 ctctacctca acttcggtca cacgattgga catgctattg aagtgaccgc tggctacggc
     3841 caggtcatgc acggtgaagc tgttgccatt ggtatggtac aaatctcacg cgcagctgag
     3901 aaaaaaggac tgatgccagc tggtatgaca gaaaagatta tcgccatgtg tgaaaaattc
     3961 ggcttgccga ctacacacca gccttggaat gtagatgaat tatatgccac cctaactcac
     4021 gataaaaaag cacgtggcaa gaccatcaaa cttgtcatcg ttcctcaatt gggacaggct
     4081 gccatccatc aaattccaat ggaagaaatg ctagaatttc tacaagtata gtcatttagt
     4141 tgacttttac gtcgaagtaa taaaagtcaa ctaatactca atgaaaatca aaaacagact
     4201 aggcgacgca gatgcagata gaactgaagt tcatcaaatc aagtcaacaa cgtctgattt
     4261 tgattttcga agagtataaa tgacgatata agaaaaaaga ctactgactt gcatgaaatc
     4321 agtagtctta ttttttataa aatcatctca tcgtcagtta gcttttcacc gctgtttttg
     4381 tagaaaaggt ccatcaagtt ttgaacagtc aagttcttct tctcttctcc ttctacatcg
     4441 acaacaatct gaccacggtg caacatgacc aaacgattac catattcaat ggcgttttcc
     4501 atattatgcg taatcatcag agctgtcaac tggtggctct cgacaatctt cttagtcaat
     4561 tccataacca tatcgctgtt ttttggatca agggcagcag tgtgttcgtc caggagcaga
     4621 acttttggtt taaccagcga agccatcatc aaggtcagag cctgacgttg accacctgaa
     4681 agaaattgcg tgtccacctt catacggttt tctagtccca ggccaagctc tttcaaggct
     4741 tccttaagaa tagcacgttc actatccttc acaccccagc caagaccacg tgacaatcca
     4801 cgacgatagg caatagccat gttttcttca atggtcaatc gagaagctgt tcccatcttt
     4861 ggatcttgaa atactcgact aatgtcctta gagcgtttgg cagctgatac atgcttaatg
     4921 gatttacctt caagcaaaat atcgcccgaa tcaaccgtca aggcacctgc aagtgagttc
     4981 atgaaagttg atttaccagc accattacca ccgatgatag agataaaatc accttctttt
     5041 acatctaagt tcaacccacg aaggacatgg ttctcattaa ctgttccagc ttcgaaggtt
     5101 ttatgaatat tttggattga taaaattgta gacataactt cctcctaagc atttccattc
     5161 aattttggac gacgaatatt caatttcttc tgcaattctg gcacatagag aacggttgcc
     5221 agaaggatag ctgagaagag tttaacaagg tctgcatcca tacttggaat ttccaaaatc
     5281 gctaagataa tcaaacggta aacaactgca ccgagaacaa ccgataggag tctccaaccc
     5341 aagcgtaagt tacgcaagat aacttctgca ataatgacag atgctagacc gataacaatg
     5401 gtaccagtac ctgagttgag atctgcatag ccattattct gagtaagaag agcaccacaa
     5461 agggcaatta gaccattcga caacatatag ccgtagattt tcatattgtc tacattgata
     5521 ccattggcct cactcattgg aatattatcc cctgttgaac gaatagctag accaatttgt
     5581 gtattgagca agagcgtcaa taagcctaca actgctagta caaagaccag accaatcacc
     5641 aaaacagcat ttgtttttgt caaaccgaaa tcctgcaatt gcgtcaccag tgtttcttgt
     5701 ttgagcaggg ctacgttggc tttgcctaag attttcaagt tgatagagta gagacctgtc
     5761 aaggttacaa taccggtcaa aagggctgga atttttagtt ttgtatgaag taaaccagaa
     5821 atcaagcctg cacccatacc agctaccata gccataaagg ttgccagcaa gggattcact
     5881 ccgttcacaa tccctgtcgc acaaacagca gctccaagcg gataagatcc ctcagctgtc
     5941 atatcagcaa tatctaaaat acgaaacgtc aagtaaacac caatcgccat aactgaccaa
     6001 agcaagcctt gcgagatact tgataaaata atatccacga ttttctcctt tttctaaatt
     6061 cgtagtcaat actaggttct atcttaccga tcttattcag atacagcaag cttagaagta
     6121 tcgataccta atttctgagc catttcttca ttaacgtgaa gactgacggt ctctgggtat
     6181 tctactgctg tttctgacac attggctccc tcgataatct taatagccat ttttgcagtt
     6241 tgacgaccta aagcttcata gttggtaccg taagtcaaaa gacctccttc atccaccatg
     6301 tccgttgaac caccgattac tggaacctta tgctccacag ataattcacc gatcatggtc
     6361 atggttgaag cgacagtatt gtcggttgga ataaagagag catctacatc cttcatcaag
     6421 ctagttgtcg catcttggac ttcatttgaa gatgtgattc cttttactac taccttataa
     6481 cctgcttttt ctagcaattc cttagcttgc tctacctgaa cttctgaatt acgttcgcta
     6541 gtagtgtaca aaataccaac ggtcttagca tctggaacag cttgacctag caattcaact
     6601 tgtttatcaa ttggagcttg gtcagatgta cctgtcaaaa gtccacctgg tttttcaatg
     6661 gattctacca agccagctga caagggatct gtaaccgcag taaagacaat cggcgtttca
     6721 ttagacacag tcgctaaact ctgggcagac ggcgtagcga ttgccaagac aatatcattt
     6781 ccatcgatta attgttctga aatggtttga aggttagctt gatcgccttg ggcattttga
     6841 taatccaaaa ccaatttttc accctcttta taaccatttg cttctaattc cgccacaaaa
     6901 ccctcacggg cagctgtcaa tgattcatgc tccatatatt ggagaactcc aactttgacc
     6961 acatctgagg aagcttgttc tgatgatgag gaacaagctg caagtgttag caaacccata
     7021 cttgctactg aaatagttgc caattttttt actagcttat aatacataaa cgtttcttct
     7081 cctaaatata ttactcttct aatgcttcta tagtcgctgc atcaatacca acagctttag
     7141 ccatttcttc attgacggta atagctgctg tatttggttt ttcaactgct aagtcagcag
     7201 gcttttcacc cttcaaaatc ttcacagcca attcaccagc ttgaacaccg atagcatggt
     7261 agtcgacacc gtaggtaaat agagctgcgt caataactgc cgcgtcactt cccatagctg
     7321 gaacttttgc ttcttttaaa atatcaccga ttgttgaagc tgtagaagca actgtgttat
     7381 cagttggaag atagatagca tcgacttttc ctgcaagtga agtaatagct tgctgaacat
     7441 catttgtcgt cgtaactgtt gtcacttcta cttttacacc ttttgcttcg agtgcttttt
     7501 tagcctgctc agcttgaact tctgagttta cctcgctaga attgtagaaa ataccgacag
     7561 tcttcgcagt tggtacaact ttgagcaaca tctcgatttg accttccaca tcagttgcat
     7621 cagtcgatcc cgtcatggtc ccacctggtt tttccaatga ttcaaccaaa cctgcttcaa
     7681 ctggatctgt gacagctgtg aatacacttg atgtttcact atctgcatta agcatggctt
     7741 gggctgccgg tgttgcgatg gcaaagttaa gatcattttt tcctgccaat ttctcaacca
     7801 ttgtctgtaa gtttgcttga tcaccttggg cattttgcaa atcaatcgtc agattttcac
     7861 cttccttgta gcctgcttct tcgagcacct ctacaaagcc ttcacgagct gagctcaacg
     7921 cttcatgttc agcatattga ataataccaa cattgactga acttgaatct gacgaattgg
     7981 aactgcatgc agcaagcgtt actgctgaca aaagtgctac tgaactaagt ataatttttt
     8041 taaatcccat gattattctc cttttataaa tataatagca aacaagaaag gaccgcttag
     8101 actacactct aaacggtcct tatatttcat ctgtatagga aaacagaatg aattatccgt
     8161 gccatttaga tgtatcatct atgaggcaca tgtcaaaata gctacagcca catagataca
     8221 aactttacgt tcccgtgttt gcatccatgt ttaccatgtc tacaaacact atctaaaata
     8281 gccgtagaaa taagtatgat tagctgaatg attttgacac atttgtaaca tctctgtttc
     8341 cctttctcat ttatttgtaa ctatcataac tgttttcaaa ttatttgtca ataggaaatt
     8401 gcgtaaattt tcagattttt caagtttttt cgaatttttc taaaaatagc cccctatatt
     8461 attagctata aaacgacctg tcatcatgtt tatgtagcct tttagtttac ttttagtact
     8521 tgctccaact atttcacaaa tagtcagagg ttggaaataa ggggaacaag gtttgcgtca
     8581 acaaccgcag ctataactca atttctagca ttgacttgaa tcagtcccct agattgcttg
     8641 cacagtaaat ggactgttca aggtgagaga tataagagcg gattgcaaaa tcaggtcaac
     8701 aacgactgat tttgattttc gaagagtata aatgactata tttgatgtaa caaaaaagtc
     8761 tgaacctaag cccagaccct actaaaacaa ttattttact tcaacttttt cagtccaagg
     8821 acacgctgta gttgctagaa cctcgtttag ttcttctacg ttgcggcggc cttgatccgc
     8881 caaccaagct ttggctgctt cttcaccctc gcttgcaaag atggctactg catccttcca
     8941 agtcgcccga ccacaaagaa caccattaaa gcttgaacca gcttctttgg caaagacaag
     9001 ggtttcttgg aaaagtttag cagatacgcc agcgctcaaa taaatgaatg gcaagtgagt
     9061 gctatctgtt tgttccttga agaaggcctt agcttcctca actgtataga ctggctcttc
     9121 atctgtgtaa ccttctacaa agttcatatt gacaggtact tcaaccttga gcacgtcggc
     9181 attataacgt gacttgctaa attcacgcat ggcaccattg accttgtgtg gtttgagggc
     9241 tgcgtattca cgagaagtca catccatatc gctggcatca taagtcaaga tttcgacgaa
     9301 gtaaggaata tcttctgcga tacactcact accaatgcgt tctacccaag catgcttaat
     9361 gtcgttgatt tctggcttgt catctacatc atagtaaagc aagattttta cggcatccgc
     9421 ccccaattct ttgatacgtt tggctgacca atttggcaaa aggtctggca aacgacctgg
     9481 tgtggacgca tcataacctg ttttttcata agctgcaatg aaaccgcaat ctgcatggcg
     9541 caattctgaa gctggaacac cgtattccgc atccaagaga atggagcttg catacggagt
     9601 caaatcgctt gagatgactt tcttgaagtc aatcaaaatg tcatctccaa attcgccacc
     9661 acctgctgca gcagcaagca ggcgtttcaa tgcaccacgt tggtcaatcg ccaaggccgc
     9721 aatcacctga tcgctgtttg acaagcgggt catgtgattg aattttgcct gagaaagttg
     9781 taattttgtc attagtttcc tcctaaggaa ttttattaac caaatcgttt cacgccatct
     9841 agataggtcg cttgtaagcg tcctgcatca tctacgacga taaagtctgc agctcggcct
     9901 tctgcaatcc gaccacaaat atggtcaata ttgacagagc gagctggtgc cagagaggcc
     9961 atacgaaggg catcaggtag ggataccaag ccccatttca caacattttg cactgcttcg
    10021 atcaattcta agatagaacc tgccaaacta ccactttctt ttaaacgagc agttccatct
    10081 tttacaacaa cttcaaattc accgaggcgg gactcccctt cacccatacc gccagctcgc
    10141 atacagtccg taatcaaaac agtttcctct gctccacgtg ctttgaccac gatttctgcc
    10201 gcaactggat gaacatggtg tccatcacaa atcatttccg catagacatt ttttaaattc
    10261 aaagcagcac cgaccatacc tggttcacgg tgatgaagcc cactcatccc attgtaaaca
    10321 tggacgaaga tattggcacc agcttctact gccgcttccg cttgcgtata ggtcgcatcg
    10381 ctgtgagcca aggcagtatg aattgccttg ctgttagcaa actcgatgaa ttctttgacg
    10441 ccttcacgtt ctggagcaat agcaatttta ttgactaaac ctttagataa gctgttccag
    10501 ttgtccaact tttcaatgga cggatcgctc atgtacttag gattttgtgc tcccttgtat
    10561 ttttctgtaa agaagggacc ttctaagaaa ataccttgaa tcttggcgcc tgtttcctgc
    10621 cctgcataag tcccaatcgt ttcacaaact gcatctaaat tttcagtcgt atctgttaag
    10681 gtcgttggaa gccaagaggt caccccacaa gacaagagac cttctgaaat taccttgatc
    10741 ccttcaaaat cattgtccat aacatcgtga gaagcgtagc cgtggatatg tgtatcaact
    10801 agccctggtg ctaaatgaaa gtcagaataa tcaacaatct ccccttcagg cttttcagtc
    10861 acaatccgtc caaactgacc cttgtcagta atttccaaat aggctgattc gacctcactc
    10921 tctggtaaaa taatagattt tgcttttata aatactgtca tattatctcc tttgttgata
    10981 tatatatact tatcaaacta gttataacca tatactaaat atattgtaga acttctatta
    11041 gaaaatgtca agtaattacc ataactttat aagaaatgga tgctgtgaga aggta
//
LOCUS       DBNFEG010000060        11057 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.060, whole genome shotgun sequence.
ACCESSION   DBNFEG010000060 DBNFEG010000000
VERSION     DBNFEG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11057
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.060"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..888
                     /locus_tag="AC1PNG_001061"
     CDS             <1..888
                     /locus_tag="AC1PNG_001061"
                     /inference="COORDINATES: protein motif:HMM:NF017927.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HIP6701571.1"
                     /translation="NAMSNSDIRAKATVIREQTDGMITLFLAPVLISFLSGVAEFGLK
                     QIWGQTGTFSWGTTIVKDGVTWTHNFISVGPSIIFDFIAQCLIVTACFQLIRLVRKQR
                     TKVNFTECFTLLDGKNFLPVFSTTLLKMLVLYLASFPAALGMILIGLAFYSTIVIFGA
                     PIDYQPDFSAFFSSSYFQTGLILVCVGITCSLFVGYGLSQVSFLLYDYLENNTYSSPF
                     KLFSQSWQLMKGNKWRRFLLDLSFIGWFIGVLLTFGFLGLYVYPYYWTCQTIFYEDLK
                     AKNPLMFSTYKSVISSTVA"
     gene            1081..1917
                     /locus_tag="AC1PNG_001062"
     CDS             1081..1917
                     /locus_tag="AC1PNG_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HIP6701572.1"
                     /translation="MMTSDIVSRAMMTRERTRGTTLLFLPSILLSIVYAVPVTIFRLL
                     TFLELRQNSAVVEGRLVSSIRLTYLGELGFALLLQLFIGLSCFHLLELLRGDRQEFSL
                     KDSFSFLKTEWKKPICTTFLYYYLLLFMAGLPANLGAALFIKGFAMSSLMVLSPGAMA
                     ELAVHQPSQLMKIGAPIFLLGMIIYLFVYYTYSQVVFVLYDHLDNASYTGPLAVFRES
                     RFLMKGNYWQRIWLDLSFLGWFIGEVFTLHLLSFFVTPYYHLSRTIFYEKLKKQRLSA
                     VE"
     gene            complement(1935..2810)
                     /locus_tag="AC1PNG_001063"
     CDS             complement(1935..2810)
                     /locus_tag="AC1PNG_001063"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIP6701573.1"
                     /translation="MKTEFINEKAQSAFNLNIITSPIVAIILLLVGDSLGAVIFTPIS
                     FLLPFSETLSVSFELFAFAFISLMVILWARYIEKSPWVGLGFTKKGAGKDFLLGWGIG
                     AAMLTTCTLIMWGLGAIEFTSIQFSTKLVGEFIILILAWSIQGTTEEILTRGWMFSSL
                     SAKHNIPIGIIVSSLFFTFLHLGNNAISLIPILDLTLFAVLACLIMLKTGNIWVIGGL
                     HAAWNCFQGNVFAFPVSGTDAGQAFVQIGITGPEWLSGGKFGVEGSIISLLVQGIMIS
                     WLVYDLYFKPKTSSL"
     gene            2933..3544
                     /locus_tag="AC1PNG_001064"
     CDS             2933..3544
                     /locus_tag="AC1PNG_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HIP6701574.1"
                     /translation="MELNQWLKSFQLKDNRLVGPFFYGTLLALRFEIGPADEAEELSR
                     AIYLERAYARAVELFEQASSEYDYVLLSLLRQEDRDIDTYLWHFSSKFNFDKVPEPEL
                     IEVEDWTGDVLVFECYLFPVTDQDLKALLREIVKADHGGFHYLSASVIFLSSKDKVLY
                     HCYDDRGVDIAVVDDDKRRQLFTDCHDLLFDYDMEEMVRRMDF"
     gene            3745..6505
                     /locus_tag="AC1PNG_001065"
                     /pseudo
     CDS             3745..6505
                     /locus_tag="AC1PNG_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006532944.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
     gene            6502..8424
                     /locus_tag="AC1PNG_001066"
     CDS             6502..8424
                     /locus_tag="AC1PNG_001066"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIP6701575.1"
                     /translation="MSQLFEVTYGQKKTSVNTNELGMREMQARVCEKRNSPYLLVKAP
                     PASGKSRALMFVALDKLKRQGLKKAIVAVPEKSIGKSFRSTKLSDFGYYWDWIVKDEN
                     NLTTSGTAKSKVEKFINFIQSTDETDDVLIVTHATLRFASEQLDASDFDACLLAIDEF
                     HHVSQDDNSVLGNALREVLQKSTAHIFAMTGSYFRGDSVPILDHADEARFERVTYTYY
                     EQLEGYQYLKSFVMNYKFYRGRYTDAFPDLLPEFIDKKTIIHIPNVNSGESSKDKYGE
                     VDEILDCLGDYLHKDEHGIIHLRSKTGKILKIADLVTEDGRDLVSNYLAKMDSEDELD
                     MIIALGMAKEGFDWPFAEYALTIGYRSSLTEIVQIIGRVTRDSSNKTKAQFTNLIAEP
                     DAEDEEVQFSVNNIMKAITASLLMENVLSPSFKFKRKENDGQKANGAEIFIKGLAKPS
                     TERTQKIIEQDITDLKASVLQDDVVQAAISAGAESKVITKQLIPRVIATRYPDLTQTE
                     VEEVRQHLVADLTLSNAKKDSENNKLLKMADKFINIDDLSIDLIDSVNPFQGAYEVLS
                     RDVDSRTLSLIARVFDAKKVEFTTDELRMLYPQIKAFQAEHGHSPRRNSSDLEEERLY
                     FALAKLDELRRERDNQ"
     gene            8421..8711
                     /locus_tag="AC1PNG_001067"
     CDS             8421..8711
                     /locus_tag="AC1PNG_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701576.1"
                     /translation="MIENFATLEDIFADEAFESLVAGIRVVKVERLDPEIEKFMEICQ
                     WVKEHGREPQRSTQIKERQLFSRLKAIRADEGRRAQVSAYDELDLLGDRHDG"
     gene            8701..9579
                     /locus_tag="AC1PNG_001068"
     CDS             8701..9579
                     /locus_tag="AC1PNG_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006532941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIP6701577.1"
                     /translation="MMDDLDDILNDDSLFENFETDRTLFSTKRYQGTVRARSKSSSRT
                     RVQTGFDTYRKLFTQVHADIACGKRQIKSIEETSQGNRISRKNPIKQGNFYVDNGVML
                     YVVKIYDPQTNVEVGQSTERKYKVHIVYENGTENHVWLLSLISSLYDKKRSGRLVTEP
                     LDTISMLGEEAAKYHTTGYIYVVKYAGTDNRFLELDNLYKIGVATDIKKRLSNSQNEA
                     TYLYAPVHLVASYEIQNVSASKLEKFLHDAFSDKRVQLETASPTGKKVSVTEWFVVEL
                     EQIEQKIYELFKQLNL"
     gene            9904..10650
                     /locus_tag="AC1PNG_001069"
     CDS             9904..10650
                     /locus_tag="AC1PNG_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701578.1"
                     /translation="MPYRKLTSRDRSFIKGQTDRLLNQMNSNAAYLGTVQTYEAAKAR
                     ANQAYLAMELEDDPEKKVRLAKLVENYTAQAQAILDEWEEKNSRTEKTTRNVKLILWL
                     TVLFTLSFVFPVNIILAILFSVWFYRNYLRSSRTDQVIKAANSQSLTEEERFEDWLTE
                     KIVVLNKDKRQWTEGIIAKYDSLDEIAAGIGEMDEIDKVAGEKLLRRYQDILADIEKL
                     PDSEQKTYYLDFHQEIIADLEDLFQQLHFG"
     gene            10808..11057
                     /gene="tnpA"
                     /locus_tag="AC1PNG_001070"
                     /pseudo
     CDS             10808..11057
                     /gene="tnpA"
                     /locus_tag="AC1PNG_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148688.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         3368 a         1987 c         2438 g         3264 t
ORIGIN      
        1 aacgcaatgt ctaatagcga tattcgagct aaagcaacgg ttattcgtga acaaacagat
       61 gggatgataa ccttgttttt ggcacctgtt ttgatttctt ttctgtcagg agtggcagaa
      121 tttggcttga aacaaatctg ggggcaaact ggtactttct cctgggggac aactattgtg
      181 aaagatggtg ttacttggac gcataatttt atttctgtgg gcccgtcaat catatttgat
      241 tttattgcac aatgtttaat tgtgacagct tgttttcaac taataagact ggtgagaaag
      301 caaagaacca aggttaactt tacggagtgt ttcactttac tagatgggaa gaatttttta
      361 ccagttttta gtacgacatt attgaaaatg ctggtcttgt atttagcaag tttccctgct
      421 gctcttggaa tgatattgat aggtttagct ttctatagta ctattgtcat ctttggtgct
      481 cctattgatt atcagcctga tttttcagct tttttcagtt cgtcttattt tcaaacagga
      541 ttaatactgg tatgtgtagg gattacttgt agtttattcg taggatatgg gctcagtcag
      601 gtctcttttc ttctctatga ttatttggaa aacaatacct attcttcacc atttaaactc
      661 ttcagtcaga gctggcagtt gatgaagggg aataaatggc gccgtttcct cttggattta
      721 tcttttatcg gctggtttat tggagttctt ttgacgtttg ggtttttggg actttacgta
      781 tatccatact attggacttg tcagactata ttttatgaag atttgaaggc caagaatcca
      841 ttgatgtttt caacatataa atcagttatc tcttccacag tagcctaggg agtatggaga
      901 taacaaatta ttaggaatcc cttcgtattt tggagggatt ttcctgtttt taagagatgt
      961 tctggaaaat taatggataa agttttatac taatagtagg atggtaaagt agtccgatgt
     1021 tgatggaata tttgcgacgt gacttcataa ataaggagag aaaattggag gggaattgtg
     1081 atgatgacga gtgatatagt atcaagagcg atgatgactc gcgagcggac aagaggtaca
     1141 acactactat ttttgccctc cattttgcta tctattgttt atgctgtacc agtaacaatt
     1201 tttcgcctac taacatttct agagctccgt caaaacagtg ctgttgtgga agggcgattg
     1261 gtttcttcaa tcaggttgac ttatttaggt gagttaggct tcgctctgct gttacagctg
     1321 ttcatcggtc tgtcctgttt tcatttattg gaattgttga gaggggatag acaggagttt
     1381 tctttaaaag atagtttttc ctttctaaag acagaatgga agaagcctat ctgtaccact
     1441 tttctttatt attatttgct tctttttatg gctggacttc cagctaatct aggggctgct
     1501 ttatttatta aaggcttcgc aatgagtagt ttgatggtct tatctccggg agcaatggca
     1561 gagctggcag ttcatcagcc tagtcaatta atgaagatag gggctccgat tttcctgcta
     1621 ggaatgatta tttacctttt tgtctactat acctactcac aggttgtttt tgtcttgtat
     1681 gaccatttgg ataatgctag ttatactggt cccctggctg tatttaggga aagtcgtttc
     1741 ttgatgaaag ggaactattg gcaacgtatt tggttagatt tatccttttt aggatggttt
     1801 atcggggaag tatttaccct tcatctgtta tctttttttg tgactcctta ctatcattta
     1861 tccaggacca ttttctacga aaaattgaaa aaacaaagac tgtcagctgt agagtagcta
     1921 acagtctttt atggttatag ggagcttgtt ttgggcttga aatagagatc gtaaaccaac
     1981 caggaaatca ttattccttg gacaagcaag ctgatgatgg agccctctac gccaaacttt
     2041 ccaccagata gccactcagg accagtgata ccgatttgaa caaaggcctg accagcatcg
     2101 gtgccgctga ctgggaaagc aaaaacattc ccttggaaac agttccacgc agcgtgtaat
     2161 ccaccaatca cccaaatgtt gcctgttttg agcataatga ggcaggcaag aacggcaaat
     2221 agggttaaat ccaaaattgg aatgagtgaa atagcattgt ttccgaggtg gagaaatgtg
     2281 aagaagaggg aagaaacaat gataccgata ggaatattgt gtttagctga aagtgatgag
     2341 aacatccatc ctcttgttag aatttcttct gtcgtacctt ggatagacca ggcaaggata
     2401 aggataataa attctccgac taattttgta gagaactgga tgcttgtaaa ttcaatcgcg
     2461 cctaatcccc acatgatgag tgtacaagtg gtcagcattg cagctccgat tccccacccg
     2521 agtagaaaat cttttcccgc acctttttta gtaaaaccaa gtcctaccca cgggcttttt
     2581 tcaatatacc gtgcccataa aatgaccatc aggctgataa aagcgaaagc gaatagttca
     2641 aaacttacag aaagggtctc gctaaatggg agtaaaaagc ttattggagt gaaaataact
     2701 gctcctagtg aatctcctac tagcaacaaa ataatagcta caataggaga ggtgatgatg
     2761 ttgagattga aagctgattg agccttctca ttgataaatt cggttttcat aggacctcct
     2821 tttactagat actttactag ttcactattc tatcatattt ttccaaagaa gactagacct
     2881 aatttcctag tatatggtag aatagtacaa agctatataa ggagaatagt atatggaatt
     2941 gaatcaatgg ctgaagtcct ttcaactgaa agataatcga ctagtgggcc cattctttta
     3001 tggcacactt cttgctctta gatttgaaat cgggcctgca gacgaagcag aagaattatc
     3061 aagagcaatc tatcttgaaa gagcctatgc acgtgcagta gaactttttg agcaggctag
     3121 ttcagaatac gactatgtgc tcctttcgct ccttcgacag gaagataggg atatagacac
     3181 ctacctctgg catttttcat cgaaatttaa cttcgacaag gtccctgagc ctgagttgat
     3241 agaagtggag gattggacgg gagatgtgct agtctttgag tgctatctct ttccagttac
     3301 cgaccaggac ctgaaggctt tgttgaggga gattgtcaaa gccgaccatg gaggttttca
     3361 ctacctttct gcttcagtta tctttctatc tagcaaggac aaggttctgt atcattgcta
     3421 tgacgatagg ggagtggata tcgcggtggt agatgatgac aaacgacgtc agctttttac
     3481 ggactgtcat gacctgcttt ttgactatga tatggaggag atggtgagga ggatggattt
     3541 ttaaaaaaac taatataaaa atgcccatga tatactacgt ataccatggg tttgactttc
     3601 agtcaaaccc taacaacaac agtcctatca caaaataaga ttaggaaact gaatggtgtg
     3661 tttagtataa taacgtgggt tattttttca tcaaacgctc cttttgtact aatacaaatg
     3721 tgttaggaaa agctaggaga tcttatggca aagtttgata ttattacaat cgaagacaag
     3781 attcagcagt tattggagga tggttttgac caagcttctt ttatctatga attcttgtct
     3841 ttttatgata tttcaaaaac agctatcacc cgaatccgta aagaacaaag tggtgaaatc
     3901 aagaacaaat tgtattacaa gacggttcct actgataagt cggttgtggc aggtgttgct
     3961 gagattgaag aagcaatcaa ggataaaaaa tcacaacccc gttatatcat tgtgaccaac
     4021 tttgtggact ttgcagccaa ggataccaag actttcgata ccttacagat cccttttaag
     4081 gatttgccga cctatgccga tttcttcttg gcttggaatg gtgtagagaa ggtagattat
     4141 gccaaggaaa atcctgcgga tattaaggct gcggaacggt tcacacgttt gtatgatgaa
     4201 ttaaagaaaa tcaatcactt tgatgaccgt agtcaagagg aaaaagagtt taacctgttt
     4261 ttgattcgct tactttttct tttatttgcc gaagatacag aaatcatgaa aaaaggtatt
     4321 tttaccaatg ccatcaaaac tcgctcgcga gaggacggca gtgatctgaa tactcttttt
     4381 aatcagatat ttgccagtct ggatattgct aatcgtctta gccaagaaaa ctggttacag
     4441 gaatttccat atgtcaatgg aaaacttttt acagatgcat caaccgatct agtttttgat
     4501 aagaaaacaa gaaaattaat cttggaagca ggcgagctcc tcaactggaa tgaaatcaat
     4561 ccggatattt taggggccat gattcagaca gtggctagca gtgagcaccg ttcaagctct
     4621 ggtatgcact atacttcggt tgaaaacatc atgaaagtga ttaaaccgct ctttcttgat
     4681 gaactgactg caaatttcca gactattgta gataaaatca atgagaacga agacaaggac
     4741 ataaccgagc gaaccaggcg agagaatagg gcggcttacg aaaaagaatt gtccacactt
     4801 cacgaacacc tatccaagat aaaattcttt gacccagcct gtggctcagg gaatttcctt
     4861 atcatcacct ataaagagct tcgtcgtttg gaaataagac tattaaaagc actacgagaa
     4921 ctacgacaag atggaaaaat tgatttcttc gaaacgtcga agattagctt gaatcagttt
     4981 tatggaattg aattagatga ttttgcacat gaagtagcta gattatctct gtggattgct
     5041 gaatatcaaa tgaatttaga agcactaaat gaaatcaatt tacaatctgc ctttctccct
     5101 ctgcgtgatg ctggaaacat cactcaagga aatgccctga gacttgactg ggataaagtt
     5161 gtccctcata ccccaaccga tgaaatatac ctgataggaa atccgcctta tattggggca
     5221 aaaattgcaa aataaagaac agaaagaaga tttacgcctg acgattggtg aacaattcaa
     5281 gtataagaaa atggactaca ttgcaggctg gttctacaaa gggattgatt tcataaatac
     5341 ttcgaatagc aaacttgctt ttgtaacaac caactctatt tttcaagggg aacaagttgc
     5401 ttttatttgg caacctgtat tatataaaat ggagatagag tttgcttata catcattcaa
     5461 gtgggggaac agcgctgcaa ataatgctgg ggtaactgtt gctatagttg gattggcaaa
     5521 taagaacaat gcaagttacc aaaaatatat ttttgatgaa accttggtac gtcaaacggt
     5581 tactcatatc aatccctact tagctagcgg ggaagatata ctggttgaga gtagtaatag
     5641 tccgattaat agattgccta aaatggtgtt cggtagtatg cctagagatg gcggtcatct
     5701 gattatttct gacttaaatg aaaggaataa gatagtagaa ggaaaccctg atcttgagcc
     5761 gtttattaag agatatatcg gcagtgaaga gtatatcaaa gataaaccac gatatgtgtt
     5821 ttggctgaat aaagaagaat atcaaactgt tcagaataac tcattgttag gtttaaggat
     5881 taagggtgta gcgcaggcta ggatgagtag ccctgcaaag tccactcaag atgcagcaaa
     5941 aacaccttat gcatttgttc aaaaaggtga gtgggacgaa gcttactcga ccttcaaaca
     6001 atcaggtgca gatgagtttg ttcagctctt ggttcctcga gtatcatctg aaagtcgtga
     6061 ctatgttcct atgggatttg ttggagaaga taccatcatt tctgattctg ccatggctgt
     6121 ctacaacgcc cctgtctatc tgcttggtct tctcatgtca cggcttcaca tgacctggtt
     6181 gcgggctatc ggtgggaagt tagaaactcg ttatcgatac tctgctggtc tggtttacaa
     6241 caccttccct gtaccagagt tatccactaa gcgaaaaaat cagattgaag aacaagtttt
     6301 tgaaatcctt gacttacggg aagaattagg tggtacctta gcagagcttt atcatcgtga
     6361 taccatgcca caagcactcc gagaagccca tcaggtacta gatagcattg ttgaacgcgc
     6421 ctataaggaa acaccattta ccagcgatga agaacgcctc agccacctct tgaaacgcta
     6481 taagaaaatg aaggagcaat catgagccag ctttttgaag tgacctatgg tcagaagaaa
     6541 accagcgtca ataccaatga attgggcatg cgtgagatgc aggcgcgtgt ctgtgaaaag
     6601 agaaacagcc cctacctctt ggtcaaggcc ccgccagcat ccggtaagtc tagggcactg
     6661 atgtttgtag ccttggataa gttgaaacgc cagggcttga aaaaagctat cgtagccgta
     6721 ccagaaaagt ccatcggcaa gtcttttcgt tcgaccaaac tttcggactt tggctattac
     6781 tgggattgga tagtcaaaga tgagaacaac ttaaccacat caggtaccgc caaaagcaag
     6841 gtagaaaaat ttatcaactt tatccaatct actgatgaga cagatgatgt cttgatcgta
     6901 acccatgcca cgctccgctt tgcttcggag caactggatg ccagtgattt tgatgcttgt
     6961 ttgcttgcca ttgacgagtt tcatcatgtc tcccaggatg ataattctgt attgggcaat
     7021 gccctgcggg aagtcttgca aaaatcaacg gcccatatct ttgccatgac cggttcctat
     7081 ttccgtggag atagtgtacc tatcttggat catgcagatg aggcacgttt tgagcgtgtg
     7141 acctatactt actatgaaca gttggaaggc tatcaatacc tcaagtcctt cgtcatgaac
     7201 tacaagtttt accgtggtcg ctacacggat gcctttccag acttattgcc tgagtttatc
     7261 gacaagaaaa ccattattca tattcccaat gttaattcag gtgagtcttc aaaggataag
     7321 tatggagagg tggatgagat tttagattgt ctaggagatt acctgcataa agatgagcat
     7381 gggattattc atcttcgttc taagacaggt aagatcttga agattgcgga cttggtgact
     7441 gaggacggtc gtgatttagt cagcaattat ctggcgaaaa tggatagtga agacgagctg
     7501 gatatgatta ttgctcttgg gatggctaag gaaggtttcg actggccatt tgcagaatat
     7561 gccctgacta tcggctaccg ctcttcgctg acagagattg tccagattat cggacgggtc
     7621 acgagggaca gctcaaacaa gaccaaagcc caattcacca atctaattgc agaaccagat
     7681 gccgaagatg aggaggtcca attctctgta aataacatca tgaaagctat tactgctagc
     7741 ttgttgatgg aaaatgtttt atctccttca ttcaagttta agcgtaagga aaatgacggc
     7801 cagaaagcta acggagctga gatttttatc aagggtctag caaagccatc aacggaacgt
     7861 actcaaaaaa tcattgagca agatataact gacttaaaag cctctgtctt gcaagatgat
     7921 gtggtacagg cagctatttc tgcaggtgca gagagcaagg tcatcaccaa acagttgatt
     7981 ccaagagtga ttgcgactcg ttatccagat ttgacacaga cagaggttga agaggttcgt
     8041 cagcatttag tcgctgattt gaccttatcc aatgctaaga aagacagcga aaataataag
     8101 ctgttaaaaa tggcggataa atttatcaat attgacgact taagtataga tttgattgat
     8161 agtgtcaatc catttcaagg tgcttatgag gttctgtcca gggatgtaga tagtcgtacc
     8221 ttaagcctga ttgcacgggt atttgatgcc aaaaaagtag agtttacaac cgacgaattg
     8281 cgcatgcttt atccccagat taaggctttt caggctgagc atggacattc accaagacga
     8341 aattcctcag atttagagga agaacgactt tactttgcct tagctaagct tgatgaactc
     8401 cgtcgtgaaa gggataatca atgatagaaa attttgcaac cttagaagat atttttgcgg
     8461 atgaagcctt tgaaagcttg gttgctggga ttcgtgttgt caaagttgaa agattagacc
     8521 cagaaataga gaaatttatg gaaatctgtc aatgggttaa ggaacatgga cgagagccgc
     8581 aaagaagtac tcaaatcaaa gaacgacaat tattcagtcg actgaaggct attcgtgcag
     8641 atgaggggag aagagctcaa gttagcgcct atgatgaatt ggatttgtta ggagatagac
     8701 atgatggatg acttagatga tattttaaat gatgattcct tgtttgaaaa tttcgaaaca
     8761 gatagaactt tatttagcac taaacgctat cagggaacag ttagagctag gagtaaatct
     8821 agtagtcgta cacgtgttca aactggcttt gacacctatc ggaaattgtt tacacaggtt
     8881 catgctgata ttgcttgtgg taaacgtcag ataaaatcca tcgaggaaac cagtcaagga
     8941 aaccgtatca gtcggaaaaa tccaattaaa cagggaaatt tctatgttga taatggtgtt
     9001 atgctgtatg ttgtcaaaat ttatgaccct cagaccaatg tggaagttgg ccagtctaca
     9061 gagcgtaagt acaaggtgca tatcgtatat gaaaatggaa cggagaatca tgtttggctc
     9121 ttgagtttga tttcatcact atatgataaa aaacgttcgg gtagattggt aacagaaccc
     9181 ttggacacaa tttctatgct aggtgaggaa gccgcaaaat atcacacaac aggctacatt
     9241 tatgttgtca aatacgctgg aacagataat cgatttttag aattggataa cctctacaag
     9301 ataggggtag ctacagacat caaaaaacgg ctttcaaata gccaaaacga agcgacttac
     9361 ctgtatgctc cagtccatct ggtagccagt tatgagatcc agaatgtttc cgcaagtaag
     9421 ttagagaaat ttctacatga tgcatttagt gataaacggg ttcaactgga gactgcctca
     9481 ccaactggca aaaaagtttc tgttactgag tggtttgtgg tagaattaga acagattgaa
     9541 caaaaaattt atgagctttt taagcaacta aatctctgac atcttatcag ctaaaccagt
     9601 ggagaaatat attgttcgtt tctattttca catttgcgat ttgttatctg ctaggcatta
     9661 tcgccatagc catagctttc acattctttc gcaggaagta gaaggacgga cagatggaca
     9721 aacttgttat ttggtttttg attttactct gggtttcacc agtgattatt gttgccttgg
     9781 caggtggatt cattattttc agtctcttat ttggtagacg gaaataatgt ctttaatcat
     9841 tgcaaattca cacaacctat cgtaaaatag taatatatct aggatataca ggaaggggtt
     9901 gctatgcctt accgtaaatt aacatcaaga gatcggtctt ttatcaaagg gcagacggac
     9961 cgtttgctga atcagatgaa tagcaatgcg gcctatttgg ggacagttca gacctatgaa
    10021 gcagccaagg ctagggccaa ccaagcttat ctggcaatgg aactggaaga cgacccagag
    10081 aaaaaggtgc gactggcaaa attggttgaa aactatactg ctcaagccca ggctatattg
    10141 gatgagtggg aggagaaaaa cagtcgtaca gagaaaacga ccagaaatgt caagttgatc
    10201 ttgtggctga ccgtactgtt cacccttagc tttgtcttcc ccgtcaatat cattctagcc
    10261 attctttttt ccgtttggtt ttatcggaat taccttcgtt caagtagaac tgaccaagtg
    10321 attaaggctg ctaatagcca atctttgaca gaagaggaaa ggtttgaaga ctggttgaca
    10381 gaaaagattg tcgtcctcaa caaggacaag cgccagtgga cagaggggat aatcgcaaaa
    10441 tatgacagtt tagatgaaat tgcagcaggt atcggtgaaa tggatgagat agataaagta
    10501 gctggtgaga agcttcttcg acgctatcag gacattttag cagatattga aaaactgcca
    10561 gattctgaac agaaaaccta ttatttggat tttcatcagg aaatcattgc cgacttggaa
    10621 gatctattcc aacaactcca ttttggataa gaaaaaccgg taacagtaca aggctgttgc
    10681 cggttttgtt atgtgacttt tagtccgtcg cgctccgtaa ggtgcgcgac gaaggttata
    10741 ccaaaaaaac tccaaacgcg atacaataaa ggtgttcaag ccaattgtaa agcgagagga
    10801 gaaaagtatg gcacaaaagg cacatagttt atcacatata aagtggttgt gttctaccac
    10861 attgtcttca cacctaagta tagacgaaaa atcatctata atcaatatcg aagtagtctt
    10921 ggagagatat ttcgacgttt gtgtagttat aaaggtgtag aaattatcga aggacatctg
    10981 atgccggatc atgttcatat gttagtcagt attcctccgc gattaagtgt atcaagcttt
    11041 atgggctatt taaaagg
//
LOCUS       DBNFEG010000061        10971 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.061, whole genome shotgun sequence.
ACCESSION   DBNFEG010000061 DBNFEG010000000
VERSION     DBNFEG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10971
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.061"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(3..485)
                     /locus_tag="AC1PNG_001071"
     CDS             complement(3..485)
                     /locus_tag="AC1PNG_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIP6701579.1"
                     /translation="MVAYKQDKEYMDYVGHLIATPKVQKLGKIPHHYYSTRLEHSINV
                     SYTSYKIAKKFGWDAKSTARGGLLHDLFFYDWRDTKFNKSHAWVHPRIAKRNAQKLIQ
                     LNKLEEDIIVKHMFGATIAPPRYKESWIVTCVDKYWAVREWSLPMQHKWKNRKVFRFQ
                     "
     gene            complement(533..1273)
                     /locus_tag="AC1PNG_001072"
     CDS             complement(533..1273)
                     /locus_tag="AC1PNG_001072"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIP6701580.1"
                     /translation="MDIIRSKTNNLVKQVKKLQQKKYRTSSYLIEGWHLLEEAMEAGA
                     NIEHIFVVEEYFEKVAGLANVTVVSPEIMQDLADSKTPQGVVAQLALPSQLLPETLDG
                     KFLVLEDVQDPGNVGTMIRTADAAGFDGVFLSDKSADIYNMKVLRSMQGSHFHLPVYR
                     MPISSILTALKSNQIKILATTLSSQSVDYKEITPHSSFALLMGNEGQGISDLVADEAD
                     QLVHITMPGQAESLNVAIAAGILLFSFI"
     gene            1438..1719
                     /locus_tag="AC1PNG_001073"
     CDS             1438..1719
                     /locus_tag="AC1PNG_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIP6701581.1"
                     /translation="MRKVKMIASGIVQGVGFRWSVQYLAMEIGDIAGRVWNNEDGTVT
                     ILAQSENAEKLSHFIHEIRKGPSRMAKVTYLDVTLANFEDYKDFQVSYR"
     gene            1789..2703
                     /gene="yidC"
                     /locus_tag="AC1PNG_001074"
     CDS             1789..2703
                     /gene="yidC"
                     /locus_tag="AC1PNG_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIP6701582.1"
                     /translation="MKNKRLFMLSGMALSTLLFLSGCVQTDKSGNPTGLIWDWLGQPM
                     SNLIRFFAEDQALGFGLAIIIVTLLVRFIILPLGIYQSWKAAIQSEKMNYLKPILGPI
                     QERMKNASSQEEQLAAQQEYFATQKQYGVSMLGGIGCLPMLIQMPFFSAIYFAARHTP
                     GISEATFLGINLGSTSLILTVVAGILYYAQSLLMQVGMDEEQKKQMKTMALMNPIMIM
                     MFSFGSPAGVTLYWVVGGLFGILQQAITNFILKPRIRKQVEEEFKNITVSATPRKTKD
                     VTPTASAVIEEKTSNKKRNRNAGKQRSR"
     gene            complement(2787..3269)
                     /gene="greA"
                     /locus_tag="AC1PNG_001075"
     CDS             complement(2787..3269)
                     /gene="greA"
                     /locus_tag="AC1PNG_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIP6701583.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEIVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISTIETKIRYAEIVNSDAVAADEVAIGKTVTVQEVGETE
                     EEVYHIVGAAGADAFANKISNESPIGHALIGKKTGDVATIETPAGSYDVKILKVEKTK
                     "
     gene            complement(3343..5160)
                     /gene="mltG"
                     /locus_tag="AC1PNG_001076"
     CDS             complement(3343..5160)
                     /gene="mltG"
                     /locus_tag="AC1PNG_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIP6701584.1"
                     /translation="MTKDTNEKNTQSSGFRDQILRDLEELKVKRLAEQSADVLVDKRN
                     EVINELQPSLAEEKAPHRETEIVKVEGIAFPDSYLSEEQQESEVMTESIEEPTSETLA
                     TPETFVIEKEVVSSPVPQDTVERNLEKLRNLVAANTAGFDEVPSSSSVSEPVESVLPG
                     NSPLEDTFLEFPTEDVSAVTGDTEIISLEETIIAPKTTAIVPEVDKQMQETQPRRRTT
                     HRTSKQRRKKQDNAAKHIVSVIMSIVVVAVLVTGVTGYMWVKSSLEPVDAKATETIQV
                     EIPEGTSTLEIGKILVDNKLIKNATIFNYYSKIKSYNNFQSGFYNLKQNMSVDDIAKA
                     LQESGTPTAQKEAAGKVLIVEGYTLTQIAQAITDNTNTKDKNDKTPFTAEQFMATVTN
                     QDFINRMVATYPKLFASLPAADSGVIYQLEGYLFPAVYEYSDETTIEELVEQMIAAMD
                     NRLQPYYETITAKNLTVNEVLTLASLVEKEGSTDEDRRNIASVFFNRLNAAMPLQSNI
                     AILYAQGKLGQETTLAEDAAIDTSIESPYNIYWTPGLMPGPVDSPSLSAIEAVINANT
                     TDYLYFVADVTTGNVYFTNNIDEHNQNVAKYVNAHLNNE"
     gene            complement(5232..5717)
                     /locus_tag="AC1PNG_001077"
     CDS             complement(5232..5717)
                     /locus_tag="AC1PNG_001077"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6701585.1"
                     /translation="MEIRFATPSDLEQVVLIENANFSKEERIAESVLATYLNTLSKTC
                     LIMEHDGEIAGYLLSCPSVSQTVTDDIFYLTESDMPTGSHLAIASLSVADSYKGQGVG
                     TLLLAAIKEVALAGGFEGVSLTCKGYLISYYEMNQFEDFGPSLSQFGGQRWSDMYWKA
                     L"
     gene            complement(5773..7104)
                     /gene="murC"
                     /locus_tag="AC1PNG_001078"
     CDS             complement(5773..7104)
                     /gene="murC"
                     /locus_tag="AC1PNG_001078"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIP6701586.1"
                     /translation="MTKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGIQILPFDEKNITADVELIAGNAFRPDNNVEIAYVDAQGYTYKRYHEFLGEFMKG
                     FTSLGVAGAHGKTSTTGLLAHVMRNITDTSFLIGDGTGRGSANAQYFVFESDEYERHF
                     APYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQVKKALLVFGEDEQLRRITANA
                     PIYYYGFEENNDFVAYDLKPSTSGSQFKVRHGEEELGEFQIPTFGKHNVMNATAVIAN
                     LYIAGFDLQLVAEHLKTFGGVKRRFTEKIVNDTVIIDDFAHHPTEIIATIDAARQKYP
                     SKELVAIFQPHTFTRTIALLDEFADALNGADAVYLAQIYGSARETDNGQVKVEDLAAK
                     INKKGGLVTVENTSPLLDHDNAVYVFMGAGDIQSYEYSFERLLSNLTNNVQ"
     gene            complement(7106..7768)
                     /locus_tag="AC1PNG_001079"
     CDS             complement(7106..7768)
                     /locus_tag="AC1PNG_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701587.1"
                     /translation="MIRHEKRFPLVADDEVLVGENPIMSLYDESDLISNIRGLYQEKE
                     FSWSTESQRVASAKPVAQTEDELLPPLFEAKPSHYSRKERLQQLTKTKPSPVKTQGQL
                     AREQAREDLKKKRSATYLRDEKPAPAKVVVKPPVVSTTESKAGRLTRLADNLRQTDYI
                     LADMPAVYSLKKEDREQEQVIKKNSYDFLRKSQVYNYPERRQQRDRQVAQELNLTHIE
                     EE"
     gene            complement(7847..10939)
                     /locus_tag="AC1PNG_001080"
     CDS             complement(7847..10939)
                     /locus_tag="AC1PNG_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HIP6701588.1"
                     /translation="MGRMMPGRIRQEGIDLYEAGKLTVLHSENGKMVLDIAGERFTYG
                     DADSDLQCSCQLFQSKGYCQHLAATEYFLKNDASGKDMKQSLKEDSEQHKETVRRTYF
                     GGLFLDEILRPEPELGLKYQLSVEGSLLPYDRQIDWTLKITRLPDTRSYIVRDIGAFL
                     RIVKANGHYQIGKNYYEQVTYENFDEPSQALLDFLWALVPEKVGIDSDILTHFGRHFR
                     LPQAYFEEGLELLNQLEHFNFSYQQQSYSSLKVLPLTGEEGLYHFEVTVHTQMIEMVI
                     HEKTVRPLFHGRYLLVNGTVYSVNRHQEHLIYQISELVPTESGLRKVQVDFPDQNRLA
                     LSLLDLQTIGTVKAPKRFIIHDFKPEFHIQMEANESLSLQLVLDFDGRKVRSEEDLAL
                     LPFASHFQHLERVYQAIRLAGFRGTYHAQRAALSQQELYPFFQQQLPILQKMGQVHLA
                     ERLQALYIEAKPQLEIVRNGSLLDISFDLSGIEQSEIDQAITALLNQEDHYTSPSGKV
                     FVFDEETKKISQTLIYLRARHGKEGQVQVHALAGYQLAQSLSQFNQVSFSKEFEEMAS
                     YLAQPDLFPLPAVEVTTSLRDYQQTGLKWLTMLDTYGFGGILADDMGLGKTLQTIAFL
                     SSRMTKDSKVLILAPSSLIYNWLDECKRFAPSLDVAVVHGNKEQREEIIANGHQVLVT
                     SYPSFRQDVALYKQERFDYLILDEAQVMKNAQSKIAQLLREFEVGNCFALSGTPIENH
                     LTELWSIFQIVLPGLLPGKQDFGKLAAKDIARTIQPFVLRRHKEDVLQELPDLIEVNV
                     LNELTDEQKAIYLAQLQQMRTQIAGADDAQINRSKIEILSGITRLRQICDTPSLFMEE
                     FSGESGKLNSLKELLLQLKEGEHRVLIFSQFRNMLEKIEEQLVEIGMTSYTLTGSTPA
                     NQRQEMTQAFNAGSRDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAISRAHR
                     MGQTEKVECYRLITRGTIEEKIQELQENKKNLVKTVLDGNESRANLTVEDIREILGIE
                     "
BASE COUNT         3117 a         2432 c         2030 g         3392 t
ORIGIN      
        1 tcttattgaa aacgaaagac cttgcgattt ttccacttgt gttgcatagg cagactccat
       61 tctcttactg cccaatactt gtcaacacaa gtcacaatcc aagactcttt atagcgagga
      121 ggagcaatcg ttgcaccaaa catgtgcttc acaataatat cttcttctaa tttattaagt
      181 tgaatgagtt tctgagcatt gcgcttagca atccgtggat gaacccaagc atgactttta
      241 ttaaacttag tatctctcca gtcatagaaa aagagatcgt gtaatagccc accgcgagcg
      301 gtcgattttg catcccatcc gaactttttc gcaattttat agcttgtata agatacgtta
      361 atggaatgtt ccaaccgtgt ggagtaataa tgatgaggaa tcttccccaa tttttgaact
      421 ttaggtgtag caattaaatg ccccacatag tccatgtatt ctttatcttg tttatatgca
      481 accattcttt gaacctcgtt ggattattat accacactat agtacaattg gcttaaataa
      541 agctaaatag taaaattcct gctgcaatcg ccacattgag actttcagcc tgtcctggca
      601 tggtgatgtg aacaagttga tccgcctcat cagccactaa atcagaaatt ccctgacctt
      661 cgtttcccat gagcaaggca aaactagaat gtggcgtaat ttccttatag tcaaccgact
      721 gactagatag ggtcgttgcc aagattttta tctgattgct tttaagagct gtaagaatgg
      781 aggaaattgg catccgataa actggcaaat ggaagtgact cccctgcatg gaacgaagca
      841 ctttcatatt ataaatatca gccgacttat ccgatagaaa tactccatca aaaccagctg
      901 catcagctgt acgaatcatt gtcccaacat ttccagggtc ctgcacatct tccagaacca
      961 aaaattttcc atccaaagtc tcaggtagaa gttggctcgg caaagcaagt tgagctacta
     1021 ccccctgagg tgtcttagaa tcagctaaat cttgcataat ttcaggactg acaactgtca
     1081 cattggctaa acctgcaacc ttttcaaaat actcttccac cacaaaaata tgctcaatat
     1141 tggctccagc ctccatcgct tcctccagca aatgccagcc ctcaatcaga taagaagatg
     1201 tccgatattt tttctgttgt aattttttaa cttgtttgac caaattgttg gtctttgagc
     1261 gaatgatgtc catgtttgtc ccttttcttt ttctgttcaa caactttatt aataaaatac
     1321 cgggtacgct tgataccaaa tatatcttat tccgtcaaga aatcatcatc aagatggtga
     1381 acaggattac ccaaccatga tataataaag taaaggtctt gtcaaaagga gaaacttatg
     1441 cgtaaggtaa aaatgattgc atctggtatt gtccaaggag ttggttttcg atggtctgtc
     1501 caataccttg ctatggaaat tggtgatata gctggtaggg tctggaacaa cgaagatggt
     1561 actgtaacca tcctcgccca atcagaaaat gctgagaagc tcagccattt tattcatgaa
     1621 atacgaaaag ggccctctcg tatggccaaa gtgacctacc tagatgttac cctggccaat
     1681 tttgaagact ataaggattt tcaggttagc tatagataaa acttgtatat ttccgtcaaa
     1741 aaaagtaaaa tagagagtat actgaaaaaa acaaaggaac gaaaaaaatt gaaaaataaa
     1801 cgtctattca tgttgtcagg catggcctta tcaacacttc ttttcttatc tggttgtgtt
     1861 caaactgata aaagcggaaa tcctacaggt ctcatttggg attggttggg acaaccaatg
     1921 tccaacttga ttcgcttctt tgcagaagat caagctctag gatttggtct tgctattatc
     1981 atcgtcacct tattggttcg attcatcatc ctcccattgg gaatttacca gtcttggaaa
     2041 gctgctatcc aatctgagaa gatgaattat ctcaaaccga ttttaggccc aatccaggag
     2101 cgcatgaaaa atgcaagctc tcaagaagag caacttgctg cacaacaaga gtattttgca
     2161 actcaaaaac aatacggtgt gagcatgctc ggcggaattg gttgtttgcc gatgctgatt
     2221 caaatgccat tcttctctgc aatctacttt gcggctcgcc acacaccagg aatttcagaa
     2281 gctactttcc ttggtattaa tttaggttca actagcctga ttttgacagt tgttgctggt
     2341 attttatact atgcccaatc gctcttaatg caagtcggca tggatgaaga acagaaaaaa
     2401 caaatgaaaa ccatggctct gatgaaccca attatgatca tgatgttctc atttgggtct
     2461 cctgctggtg tgactctcta ctgggttgtc ggtggtctct tcggtattct tcaacaagct
     2521 attacaaact tcattttgaa acctcgtatt cgtaaacaag tggaagagga attcaaaaat
     2581 atcacagtgt cagcaactcc tcgtaaaacg aaggacgtta ctccaactgc ttcagcagtt
     2641 attgaagaaa agacatctaa caagaagaga aaccgtaatg caggaaaaca acgttctaga
     2701 taataaacac taaaagattg gaatttccaa tcttttttag caccttcgtg aataaaaaga
     2761 gagaatcttt cgactctctc cacaccttat ttcgtttttt caaccttcaa gatttttaca
     2821 tcataactac cagcaggtgt ttcaattgtt gcaacatctc ctgttttttt accaatcaaa
     2881 gcatgaccaa ttgggctttc atttgaaatc ttgtttgcaa aggcatctgc acctgcagca
     2941 ccaacgatat gatagacttc ttcctcggtc tcaccaactt cttgaacagt cacagtctta
     3001 ccaattgcta cttcatccgc agcaactgca tcactgttta cgatttcagc atagcgaatt
     3061 tttgtttcga ttgttgagat ttgaccttca acaaaagcct gttcatcttt agctgcttcg
     3121 tactcagagt tttctgaaag gtctccgtaa gaacgagcaa ttttaatacg ttcaacaatt
     3181 tctggtcgac gaactagctt tagttcctct aattcttttt ctagtttttc cttttcttcc
     3241 aaggtcattg gatatgtttt ttctgccatt tgtctttcct tttcttttat ttttcatgaa
     3301 aacaaaatta tgtggaaaac cacataattt tgttttaaga atttactcat tattaagatg
     3361 tgcatttaca tacttagcaa cgttttggtt atgttcatca atattatttg taaagtaaac
     3421 atttccagtt gtaacatctg ctacaaagta aagatagtct gttgtgttgg cattaatcac
     3481 tgcctcgata gccgaaaggc ttggactatc cactggtcca ggcatcaatc ctggtgtcca
     3541 ataaatgttg tatggtgact caattgatgt atcaatcgct gcatcttctg ctagagttgt
     3601 ttcttgacca agtttaccct gagcatacaa gattgcaata ttagattgta gaggcattgc
     3661 tgcattcaag cggttaaaga agacacttgc aatgtttcga cgatcttcat cagtagaacc
     3721 ttctttttca accaaagatg ccaaggtaag aacttcattt acagtcaaat tcttagcagt
     3781 aatcgtttca taatatggtt gtaaacgatt atccatcgcg gcaatcattt gctcaacgag
     3841 ctcttcgata gttgtttcat cgctgtattc ataaaccgct gggaagaggt aaccttccaa
     3901 ctgataaata actccactat cagcagctgg taagctggca aacagttttg gataagtagc
     3961 aaccattcga ttaataaaat cttggttggt cacagtagcc ataaactgtt ctgcagtaaa
     4021 tggtgtttta tcgttcttat cttttgtatt agtattgtct gtaatagcct gagcaatttg
     4081 tgttaatgta tacccttcaa caatcaaaac cttccctgca gcttcttttt gagctgtcgg
     4141 agttccgctt tcttgaagtg ctttggcgat gtcatccaca gacatatttt gttttaaatt
     4201 ataaaaacca ctttggaaat tattataact cttaatttta gagtagtaat taaaaatagt
     4261 agcattctta atcaacttgt tgtcaactaa aattttacca atttctaaag ttgaagttcc
     4321 ttctggaatc tctacctgaa tagtttctgt agcttttgca tcgactggtt ccagactaga
     4381 ttttacccac atataaccag taacacctgt cactagaact gccactacaa caatcgacat
     4441 gataacagag acaatgtgtt tagcggcatt atcttgtttt ttacgacgtt gcttgcttgt
     4501 tctgtgggtt gttcttcttc tgggttgtgt ttcctgcatc tgcttgtcta cttctggaac
     4561 aatagccgtt gtctttggag caataatagt ttcctctagt gagatgattt ctgtatcacc
     4621 agtcacagct gacacgtctt ccgttggaaa ttctaaaaat gtatcttcta aaggagaatt
     4681 tcccggaagg acagactcta ctggctcaga aactgacgag cttgatggta cttcatcaaa
     4741 tcctgccgta ttagcagcca caagattcct taatttctcc aagtttcgtt caacagtatc
     4801 ttgtggaact ggacttgaaa caacttcttt ttcaatcaca aaagtctctg gagtagccaa
     4861 agtttctgat gttggctctt caatactttc cgtcatcact tctgattctt gctgttcttc
     4921 tgacaagtat gaatcaggga aagcaatacc ttctactttt acaatttcag tctctctatg
     4981 gggggctttt tcttcggcaa gactaggctg gagctcgttg ataacttcat tacgcttatc
     5041 aacaagaaca tccgctgact gttccgccaa acgtttcacc ttcaattctt ccaaatctcg
     5101 taaaatttga tcgcggaagc ctgaagactg cgtgtttttt tcattcgtat cctttgtcac
     5161 gctgttactc ctttcacaca gttactatac attatatctt aaaggcttat gaattgcaag
     5221 aacttccctt tctacaaggc tttccagtac atatcactcc acctttgtcc tccaaattgc
     5281 gacaaggagg ggccaaaatc ttcaaactgg ttcatttcat agtagctaat caaatacccc
     5341 ttacaggtca gggaaactcc ttcaaatcct cccgctaaag ccacttcttt tatggctgcc
     5401 aacaaaagag ttcccactcc ctgtccctta taggaatccg ctacggatag actggcaata
     5461 gctaggtgac tgcctgtcgg catgtcactc tctgtcaagt aaaaaatatc gtctgtaaca
     5521 gtctgcgaaa cagagggaca ggataataaa tatcctgcta tttccccatc gtgttccatt
     5581 attaaacagg ttttactcaa agtattcaga taagtagcaa gaacagactc agctatccgt
     5641 tcctctttag aaaaattggc attttcaatc aaaacaactt gttctaaatc cgaaggagta
     5701 gcaaaacgaa tttccataat tttctccctt atatagcgga aaaacagggg ccataggcct
     5761 ccgtctttct ttttattgga cgttatttgt aagatttgac aagagtcttt cgaaggaata
     5821 ttcataggac tgaatatcac cagctcccat aaatacatat actgcattgt catggtctaa
     5881 aagtggtgaa gtgttttcaa ctgtaacaag tccacctttt ttgttgattt tagccgccaa
     5941 atcttctacc ttaacctgac cattgtctgt ttcacgcgct gatccataaa tttgagcaag
     6001 gtaaactgca tctgctccat tcaaagcatc tgcaaactca tcgagaagcg caatggtacg
     6061 agtgaatgta tgtggttgga agatggctac caactccttg ctaggatact tctgacgtgc
     6121 cgcatcaatg gttgcaatga tttccgttgg atggtgagca aagtcatcaa tgataacagt
     6181 atcattgaca attttctccg taaagcgacg tttgacacct ccaaatgtct tcaagtgttc
     6241 tgcaaccagt tgcaaatcaa atccagcaat atagagattg gcaataacag ctgttgcatt
     6301 catcacatta tgcttaccaa aggttggaat ctggaattct cccagttctt cttcaccatg
     6361 gcgaactttg aattgtgaac cacttgtaga aggcttcaag tcataggcaa caaaatcgtt
     6421 gttttcctca aaaccatagt aataaattgg agcatttgcc gtgatacgac gaagttgctc
     6481 atcttcacca aaaacgagga gagctttttt tacttgcttc gcataatcat tgaaagcatt
     6541 gaaaacatcc tctagactag tgaaataatc tggatggtca aaatcaatat tggtaatgat
     6601 gctgtattca ggatggtatg gggcaaagtg acgttcgtat tcatcggatt cgaacacaaa
     6661 atactgagca tttgccgaac cgcgaccagt accatcacca atcaagaagg aagtatctgt
     6721 gatattgcgc ataacatgtg ccaataatcc cgttgtagag gtcttaccat gggcaccagc
     6781 aacaccgagg ctcgtaaaac ctttcataaa ttcacctaga aattcatggt aacgtttgta
     6841 ggtatagccc tgggcatcaa cataagcgat ttccacattg ttatctggac gaaaagcatt
     6901 accagcaatc aattcaacat ctgctgtaat atttttctca tcaaacggta aaatctgaat
     6961 cccagcttgt tcaagcccgc gttgtgtaaa gtagtatttc tctacatcac taccttgaac
     7021 cttgtgcccc atttgatgca acataagagc aagtgcgctc atacctgatc ccttaattcc
     7081 gataaaatga taggtttttg tcatgttatt cctcttctat atgtgtcaaa ttcaattctt
     7141 gtgccacttg tcgatctcgt tgttgtctgc gctcaggata attgtaaacc tgactttttc
     7201 ttaaaaagtc atacgaattt ttctttataa cctgctcctg ttctctatct tctttcttca
     7261 aagaatagac agctggcata tcagcgagaa tatagtctgt ctgacgtaag ttgtccgcca
     7321 aacgtgtcaa ccgacctgcc ttagattcag ttgttgaaac gacaggaggt ttcaccacaa
     7381 ctttagctgg tgctggtttt tcatctctta agtaggttgc cgatcgtttt ttcttcaagt
     7441 cttctctagc ctgttcgcga gccaactgcc cctgtgtttt cactggagaa ggcttcgttt
     7501 tagtcagttg ctgaaggcgt tcctttcgag aataatggct tggcttagct tcaaacagag
     7561 gaggcaggag ctcatcttcc gtttgtgcta ctggttttgc cgacgcaact cgctgtgatt
     7621 cggtagacca gctaaattcc ttttcttgat aaagccccct gatattgcta atcaggtcgc
     7681 tctcatcata taaactcata attggatttt ctccaactag tacctcatca tctgcgacta
     7741 gtggaaatct tttttcatgt ctaatcataa gtcatccttt cgaaccctta ttataccata
     7801 cttaggcctt atttcctaat tctacgctga gtcaactgat tccatatcac tcaatcccta
     7861 aaatctcacg aatatcctca accgtcaaat tcgctcggct ttcattgcca tcaaggactg
     7921 ttttgacaag gttcttttta ttttcttgca attcttgaat cttctcttcg attgtccctc
     7981 tagtaatcag gcgatagcat tcaacctttt cagtctgccc catgcggtga gcccggctga
     8041 tagcctgtgc ttccacggca ggattccacc aaaggtcgac taggatgact gtgtctgcac
     8101 ctgtcaaatt caagccaacg ccacctgctt tcaaggaaat gagaaaggca tctcgacttc
     8161 ctgcattgaa ggcctgtgtc atttcttggc gttggttagc aggtgttgaa ccagtcagag
     8221 tataggatgt catcccaatt tctaccagct gttcttctat tttttccagc atatttctga
     8281 attgggagaa tatcaagacc ctgtgttcac cttcttttag ctgcaagagt aattctttca
     8341 aactattaag tttgccactc tcaccactaa actcttccat gaaaaggctg ggggtatcgc
     8401 agatttgacg taagcgagta attcctgata ggatttctat cttgctacga ttgatttgag
     8461 catcatctgc gccagctatt tgagtccgca tttgttgaag ctgggctagg tagatggctt
     8521 tttgttcatc tgtcagttca ttaagtacat tgacctctat caaatcaggc aattcctgaa
     8581 gaacgtcttc cttatgacgt ctgagaacga agggttgaat ggtccgcgca atgtccttgg
     8641 ctgctaattt accgaagtct tgtttgcctg gcaataaacc tggtaagaca atttggaaaa
     8701 tggaccagag ttccgtcaaa tggttttcaa tcggcgtacc tgagagggca aagcaattcc
     8761 caacctcaaa ctcacgcagt agctgtgcaa tcttagattg ggcattcttc atgacctgcg
     8821 cctcatctaa aatgaggtag tcaaagcgtt cctgtttata gagtgcaaca tcctgtctga
     8881 aggatggata ggaggtaact aagacttggt gaccattggc aatgatctct tcacgctgtt
     8941 ccttgtttcc atggacaaca gccacatcta aacttggagc aaaccgctta cactcatcta
     9001 accagttata aatcaagcta gacggtgcca atattaaaac tttggaatct tttgtcatcc
     9061 gactggacag aaaggcgata gtttgcaagg tctttccaag ccccatgtcg tcagctaaaa
     9121 ttccgccaaa accatacgta tccagcatag tcaaccactt aagccctgtc tgctgataat
     9181 ccctaagcga cgttgttact tccacagcag gaagcgggaa caaatctggt tgagctagat
     9241 agcttgccat ttcctcaaat tccttggaga aactgacttg gttgaattgt gacaaggatt
     9301 gagccaattg gtaaccggca agagcatgga cttgtacctg cccctcctta ccatgacggg
     9361 cccgcaaata gattagggtt tgactgattt tcttggtttc ctcatcaaaa acaaatacct
     9421 taccgctcgg gctagtatag tggtcctctt gattcagcaa ggctgtaatc gcctgatcaa
     9481 tttcagattg ttcaatacct gataagtcaa acgaaatatc caaaagtgag ccgttacgca
     9541 caatttctaa ttgaggtttg gcttcaatat ataaagcctg tagcctttct gctagatgaa
     9601 cctgccccat tttttgtaat ataggcaatt gttgttggaa aaatggatag agctcctgct
     9661 gactaagagc cgctctctgg gcatgatatg tccccctaaa gccagctaaa cgaatagcct
     9721 gatagaccct ctccaaatgt tggaaatggc tggcaaaagg caggagagcc aaatcctctt
     9781 cactccttac cttacgtcca tcaaaatcca aaaccaattg gagggataaa ctttcatttg
     9841 cctccatctg aatatgaaat tctggcttga aatcatgaat gatgaagcgt ttaggtgcct
     9901 taacagtccc aattgtctgc aaatccagca agcttaaagc cagacgattt tggtcaggga
     9961 aatccacctg cacttttctc aaacctgatt cggttggaac aagttcggaa atttggtaaa
    10021 tcagatgttc ttgatggcga ttgacactat aaaccgtgcc atttactaag agatagcgcc
    10081 catgaaaaag aggtcggact gttttttcgt gaataaccat ttcaatcatc tgcgtgtgaa
    10141 cggtcacctc aaaatgatag agcccttcct cccctgtcaa cggaagaacc ttcaaggagg
    10201 aataggactg ctgttgatag gaaaagttaa aatgttctag ctgatttaac agctctaacc
    10261 cctcctcaaa ataggcttgt ggcagacgaa aatgtcgtcc aaaatgagta agaatatccg
    10321 aatctatacc aactttctct ggtaccaagg cccagagaaa atcaaggaga gcctgacttg
    10381 gttcatcaaa attttcataa gtaacttgct cataataatt tttacctatt tgatagtgac
    10441 cgttggcttt cacaatgcgc aaaaaagctc cgatgtcccg cacaatgtac gaacgtgtat
    10501 ctggcaaacg ggtaattttc aaggtccaat ctatctgacg atcatagggc aataagctcc
    10561 cttcaactga caactgatac ttaagcccca gttcaggttc tggccgcaaa atttcatcta
    10621 aaaagagacc gccaaaataa gtccgacgga cggtttcctt atgttgttcg ctatcttctt
    10681 tcaaagactg cttcatatcc ttgcctgagg catcattttt taaaaaatac tccgtggcag
    10741 ctaggtgttg acagtagccc ttactttgaa ataactggca actacactgt aaatcgctat
    10801 ctgcatcgcc gtaagtaaag cgctcaccag caatatccag caccatcttt ccattctcac
    10861 tatgaagaac agtcagtttt ccagcctcgt acaaatcaat tccttcttgg cgaatccgcc
    10921 ctggcatcat gcgacccatt ctaactcctt tatccttcaa ccaatcatca a
//
LOCUS       DBNFEG010000062        10966 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.062, whole genome shotgun sequence.
ACCESSION   DBNFEG010000062 DBNFEG010000000
VERSION     DBNFEG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10966
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.062"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1427
                     /locus_tag="AC1PNG_001081"
     CDS             <1..1427
                     /locus_tag="AC1PNG_001081"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HIP6701589.1"
                     /translation="KTVLAIDLGATSGRGILYSIENGKLISREVHRFNNSIQKVKGRL
                     CWDIESLVKSVKKCIRISQEQSELSSVGIDTWGVDFALLDVNGEMIAQPVSYRDRRTE
                     SILDEIARYSSLNDLYFKTGNQLMEINTLFQLIACKSQTPEEYFKADKLLMISDYLNF
                     VLTGEVAIERSIASTMQMINPLTQDWNHEILKTFEISELLLPKLVSEGNILGSISDEI
                     LNNEITVINVCQHDTASAVAAIPHEEENLLYVSCGTWSLIGTELKGPILTDSALKYNF
                     TNEAGYDGTTRFLKNCTGLWIIEELKKSFLKLGKDFSYDEITEMVNNVESCVPTFDTD
                     ASTFASPGNMIEKIVCYFSDKGIKVSTDPAILFKIVYQSLAEKYKEVIIQLEEITGNI
                     YKRIDLIGGGSKSEYFSQLVADVTGKTVVTGLYEATSIGNALVQFKALGYVEDMKEAK
                     KLVKESITFNHFYPKKEEKNDTLS"
     gene            1411..3198
                     /locus_tag="AC1PNG_001082"
     CDS             1411..3198
                     /locus_tag="AC1PNG_001082"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HIP6701590.1"
                     /translation="MTLYPKIGIRPTIDGRQGGVRESLEEKTMQMAMAAKDLIESNLR
                     YADGTPVQCLLASRTIGGQGDAGIVYDEFARDNVVATLSVTPSWCYGTETMDMDPHTI
                     KAIWGFNGTERPGAVYLAAAMSGYAQKGYPAFKIYGHDVQDLDDNSIPADVAEKILLF
                     AKGAIAAGQMKGKSYVNIGASSMGIAGSQVTTSFFEDYLGMLVEFVDMTEILRRMELE
                     IYDKEEYEKALAWIKEYCKEGVDINAGKEFPEVITKSKVIPADKDWEFIAKQTLIIRD
                     ILFGNPRLAEIGWEEEARGRNAISGGFQGQRQWTDWLPNGDFAEAILASTFDWNGSRP
                     VTAFATENDTLNATAMLFGTLLTNKAPIFSDVRTCWSPEAVKRVTGQELTGRAKDGIL
                     HLINSGASALDGSAAARDEAGQPTMKEFWNMTEEDIKACLEATDWCRANYEYFRGGGY
                     SSHFKTQGELPVTLIRVNLVKGVGPSLQIAEGYTCPLEDEVHRILDERTDKTWPTTWF
                     APNLGEPGFETVYDVMNHWGSNHGAFVHGHIGAEVITLASMLRIPVALHNIPRERIFR
                     PSIFDGVGTKDLEAADFKMCEVLGPLYSK"
     gene            3213..3857
                     /locus_tag="AC1PNG_001083"
     CDS             3213..3857
                     /locus_tag="AC1PNG_001083"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HIP6701591.1"
                     /translation="MFMEKERKELIKYGKKLVTEGLTKGTGGNLSVFNREKGLMAITP
                     SGIDFFEIKEEDIVIMDLEGQVVEGEKLPSSEWYMHLIQYQQREDLDAVIHAHTTYGT
                     VLAVLREPLPASHYMIAVAGKDVRVAEYATYGTKELAENAAEAMKDRRAVFLANHGIL
                     AGAQDLLNAFNIIEEVEYCSKIYCVAKSMGEPVILPDEEMELMAEKFKTYGQRK"
     gene            3874..5172
                     /locus_tag="AC1PNG_001084"
     CDS             3874..5172
                     /locus_tag="AC1PNG_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP6701592.1"
                     /translation="MKKGLLKCGALCLFASVILAACGSSSGTSSSSEKAASGDDTVLE
                     FYHGYHHSEEEWPAAKVMRDLYDEFAKKHATGDVEFKPTPVNGSLTDIMNNKVASGEF
                     PDMIDLAGNDVSLAAIEQSLVLDLKPYIDDNGLEKNVGLNYTQNDVNGKIYTVHDQLL
                     TLGLWYNADIFSKAGAKTPDQWTKWSDFTEAMALVRKQGVYAFGAGEPAIRLFNTVLG
                     SSEAGRKLLEGPLTSEGIDSKEFAEALKLVMTEVQANGSANAGGDANVYSTDFTENKS
                     AVFFNGVWAAGGLKDNAALKPGLYAGNVAISSAGGGITISSKLSEEEEKLALEFVKYM
                     TSDEVQTTIFEKVGANPASQAIDTAQIASGSDDATVKLLGEATSMANNADVTVPTVVS
                     VWGGDVRTAIINALTESAADGVNIDQKVKETQDILKALIG"
     gene            5227..6147
                     /locus_tag="AC1PNG_001085"
     CDS             5227..6147
                     /locus_tag="AC1PNG_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIP6701593.1"
                     /translation="MAKKTMTLQNRKAQKQRFIFLFLLPTLVCFFLFYFYSVVTIFAT
                     SFTKWDYTNLTSPEFLGFGNLFANYKYIFSEYPFFLEALKNSITWAIIGVVVQVPLAL
                     SVAIALSKKLAGWKLSRNLYIIPSIISSAAMGLIFLQIYNPNYGVVNQVIRFFNPEFS
                     DAILLTPGLNIIAMTCAYIFFAGTSTIMILGQIFAIPQEIHEAAILDNITGWRREWYI
                     TIPMIKETLKTVSIMAATSGFLLYNEVFFLTNGAAGTKSISFIIRELAVSSSRTQYAR
                     ANTIGVIQILGGMLIILCINLIFKEGKRRK"
     gene            6158..6997
                     /locus_tag="AC1PNG_001086"
     CDS             6158..6997
                     /locus_tag="AC1PNG_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIP6701594.1"
                     /translation="MDNRLNGLSKSGKLIIYIYMAITCLISVFPILWIFLSSLKADPM
                     KNPGISLPTDLSFDGYIKVFTELHVLDYFWNSFKVVSISVIISIIMISMSSYVIARMD
                     FRGKKLITAMLYSTLFIPATAMTFPVYRLVNVLGIYNTPVALIFVYSCSGIAMSFFII
                     KNYFAIIPKELEEAAEIDGASYAQTFWKVMLPIARPGILTAAILAFINNWNEYYWASM
                     LIINKDELTVPALLGQFTTSFNTNYNGLFSAIVVIILPPILLFAFASKYFIEALGGGA
                     VKG"
     gene            6994..8328
                     /locus_tag="AC1PNG_001087"
     CDS             6994..8328
                     /locus_tag="AC1PNG_001087"
                     /EC_number="3.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HIP6701595.1"
                     /translation="MTKNSLQEINKRTEWFQKDRFGMFIHWGLYSIPGKGEWIRSHQQ
                     LSIDDYQPYFEAFNPVKYNPREWAKAAKAAGMKYMVLTAKHHDGFCLFDSAYTDYKST
                     NTQIRKDLVAEFVESVREEGLKVGLYFSLIDWYHPDYPKYGDMIHPMRENEAFKDEKI
                     DFDNYLTFLHNQVEEIVTKYGKLDILWFDYSYGEMFGEKWRATDLIKLVRKHQPDVVV
                     DNRLETSGEGFGSIVTDNINLYSGDFVSPEQIVPHEGIRNIHGEPIPWELCLTMNNNW
                     AYNPTDYLYKSSRTLIRKLVECVSKNGNMILNVGPDALGIINQESLEILNDFGKWLDK
                     NGESIYGCGVSSLPKPEWGYYTQKGNTVYAHVFEQPIGPLALIGIDESKVKRMSFLHD
                     GSEVKISKSWTTNAYEGICFAQYGDIPHFTYPLPDSVDSVIKIELVGDDSED"
     gene            8318..10195
                     /locus_tag="AC1PNG_001088"
     CDS             8318..10195
                     /locus_tag="AC1PNG_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylgalactosaminidase"
                     /protein_id="HIP6701596.1"
                     /translation="MKIENKQIARYFKLEDGKFYTSHILNKKLDEPIGNEKNTEFLVS
                     FCDGSQLSSEDFTAHVVEQTNESLLVSFYHSEIQLTIFYSKREDVLAKEVTILSSSKT
                     INYIDVEQFGFANLEQVYIPKQQEDIKEMAGFSGYYVELGQPIYAKSFFFGMEFPMGE
                     NRLVDQTYFSRYYVGHKIKEPKKIWPVVIGAASGFEKASIQQEFFTYISGIAQPSYFR
                     KQYNSWYDHMTAIDEGIIVKSFEEISHGFEDNGVKLDAYVVDDGWCDYQSVWEFNEKF
                     PNGLTKVKALVNNLGASLGLWIGPRGGYNGTEVIMSDWLEAHPEFGSKNALSNDVNIG
                     DFNYLEGMKQKMLDYQKKYDISYWKIDGWLLKPDKPDPSGEFAMHTMTPVYEFLIDLL
                     KDLRAERGDRDCWLNLTSYVNPSPWFLQWVNSLWIQISQDVGFTENAGNDIQRMITYR
                     DCQYEEFLAKRDIQLPLWSLYNHEPVYASTAHTWYMDHQMYASVEEFEDYLLFISTRG
                     NAFWEFHYSYTMFDQERWKANARAVKWIENNYSTLKHSQMIGGKPSEFEIYGYRCIDE
                     DVGKEILSLRNPASHSATIQLDNINLSDYQLVRGDYKALTETEYELAPYTILIAEKLG
                     G"
     gene            10197..>10966
                     /locus_tag="AC1PNG_001089"
     CDS             10197..>10966
                     /locus_tag="AC1PNG_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIP6701597.1"
                     /translation="MAVKYALGVDIGGTKVAVGLVDGTGHVAYSLKVPSNKESSETLF
                     QCVCTTIRELLNSQQLNIEDVAGIGVGLPGKVDVENGVAVFQNNIPWENFPVVKRLQK
                     EFGNIPIKIDNDVKVAAYAEYRMLSLEADDMFGYITISTGIAATNIINNTILRGSGFA
                     GEIGFMPVRSFGRTSGLEISCSGPAIERQGKQMYGEDLSTKAVFDNWRKGDITASAII
                     ANARDGMVQAIHNMICLLDPKIIVLGGSVAQNNPDFIE"
BASE COUNT         3437 a         1832 c         2451 g         3246 t
ORIGIN      
        1 tgaaaactgt attggctatt gaccttggag ctacgtcggg aagaggaatc ctatattcta
       61 ttgaaaatgg aaaactgatt tcgagagaag ttcatcgatt caacaattca atacaaaaag
      121 tgaagggaag actgtgttgg gatatagaaa gcttggtaaa aagtgttaaa aaatgtatca
      181 gaatttctca agaacagtca gaattatctt ctgttggaat tgatacatgg ggtgttgatt
      241 ttgcattatt ggatgtaaat ggagaaatga ttgctcaacc agtatcctat cgagataggc
      301 gaactgaatc aatattagat gagattgcaa gatattcttc tctaaatgat ttgtatttta
      361 aaacgggtaa tcagttgatg gagattaaca cactatttca attgattgct tgtaaatctc
      421 aaacccccga agaatatttc aaagctgata aactgttaat gatttcagat tacttaaatt
      481 ttgtattgac aggtgaggtc gcaattgaaa gaagcattgc ttccacaatg caaatgatta
      541 acccattgac acaagactgg aatcatgaaa ttttaaaaac atttgaaatt tcagaattac
      601 ttttaccgaa actagtatcc gaaggaaata tacttggtag tatatcggac gaaatattga
      661 ataatgaaat aacagtaatc aatgtctgtc agcatgatac agcaagtgcg gtagcagcga
      721 ttccacatga agaggaaaac ttactctatg tctcttgtgg aacttggtca cttatcggga
      781 cagaactgaa aggcccaata cttaccgata gtgcactgaa gtataatttc acaaatgaag
      841 ctggctatga tgggactact agatttttga aaaattgtac tggactttgg attattgaag
      901 aattaaaaaa atcgttctta aaattaggaa aagatttttc atacgacgaa attacagaga
      961 tggtgaacaa tgtggaaagt tgtgttccaa catttgacac agatgcttcg acattcgcaa
     1021 gtccaggtaa catgattgaa aaaattgtct gttatttttc agataagggg ataaaagtat
     1081 caacagaccc agcgattcta tttaagatag tttatcaaag tttggctgaa aaatataaag
     1141 aagtcatcat acaattggaa gagataacag ggaacatcta taagagaatt gacttaatag
     1201 gcggtggttc caagtcagaa tatttctcac aattagtagc agatgttaca ggtaagacgg
     1261 tggttactgg tctgtatgaa gcaacctcaa tcggtaatgc cctcgttcaa tttaaggcac
     1321 ttggctatgt tgaagatatg aaagaagcca agaaacttgt aaaagagtct ataacattta
     1381 accattttta tccaaagaag gaggaaaaaa atgacacttt atcctaaaat tggaatccgt
     1441 ccgactattg atggtcgaca gggcggtgta agggaatcct tagaagaaaa aaccatgcaa
     1501 atggctatgg ctgctaagga tttgattgag tccaatttgc gttacgcaga cggaacccct
     1561 gttcaatgtt tgctggccag tcgaaccatt ggtggtcaag gggatgcagg gattgtctat
     1621 gatgagtttg ctcgtgacaa tgtagttgca accttatctg tcacaccaag ttggtgctat
     1681 ggaacagaaa ccatggatat ggatccacat acgattaaag ctatttgggg ctttaatggg
     1741 acagagcggc ctggagcagt ttatctagcc gcagccatga gtggctacgc tcaaaagggt
     1801 tacccagcat tcaagattta tggtcatgat gttcaggatc tggatgacaa tagcattcct
     1861 gctgatgtag ctgaaaaaat cttgctattt gccaagggag ccattgctgc tggtcagatg
     1921 aaaggcaagt cctatgtcaa cattggagct tcttcaatgg gaattgcggg ttctcaggtt
     1981 accacttcct tctttgaaga ctatctggga atgttggtcg aatttgtgga catgactgag
     2041 attcttcgcc gtatggagtt ggaaatctac gataaagaag aatatgaaaa agctcttgct
     2101 tggattaagg aatattgtaa agagggtgtt gacatcaatg caggaaagga attcccagaa
     2161 gtgattacca agtccaaggt gattccagct gacaaggatt gggaatttat tgccaagcaa
     2221 accttgatta tccgcgatat tctctttgga aatcctcgct tggcagagat tggctgggaa
     2281 gaggaagctc gtggcagaaa tgcaatttca ggtggtttcc aaggccaacg gcaatggact
     2341 gactggctac caaatggtga ttttgctgaa gcgattctgg cttccacttt tgactggaat
     2401 ggttcacgtc ctgtgactgc ctttgcaaca gaaaatgata ctttgaatgc gaccgccatg
     2461 ttatttggaa ccctcctaac caataaggct ccaattttct ctgatgttcg gacctgttgg
     2521 agcccagagg cggtcaaacg agtgactggt caagagctaa caggtagggc taaggatggc
     2581 atcctccacc ttattaactc aggagcttca gcccttgatg gaagtgcagc agcacgagat
     2641 gaagctggtc agccaaccat gaaagagttc tggaatatga cagaggagga tataaaagcc
     2701 tgtctggaag cgacagattg gtgtcgagca aactatgaat atttccgagg aggtggctat
     2761 tcttctcact tcaagactca gggggaactt cctgtgacct tgattcgtgt caatctagtc
     2821 aagggtgttg gtccaagttt acaaattgca gaaggctaca cctgtccatt ggaggatgaa
     2881 gtgcaccgga tcttggatga acggacagat aaaacctggc caacaacctg gtttgctccg
     2941 aatcttggag aacctggttt tgaaaccgtc tatgatgtca tgaaccattg gggttctaac
     3001 cacggtgcct ttgttcatgg gcacattggt gcagaagtta tcactctggc tagtatgttg
     3061 cgaattcctg tagccctcca caatattcca agggaacgaa ttttccgtcc aagcattttt
     3121 gatggtgtcg gtactaagga tttggaagcg gcagatttca agatgtgtga ggttttagga
     3181 ccgctatata gtaaatagaa ggagaacact ttatgttcat ggaaaaagaa agaaaggaac
     3241 ttataaagta cggcaaaaag ttagttactg aaggcttaac caaaggtact ggtgggaatc
     3301 tgagcgtatt taaccgtgaa aaaggtctaa tggcaatcac tccatctgga attgatttct
     3361 ttgaaatcaa ggaagaagat atcgtcatca tggacttgga aggacaagta gtcgaaggtg
     3421 aaaagcttcc ttctagtgag tggtatatgc acctgattca ataccagcaa cgagaagacc
     3481 ttgatgcagt cattcatgcc cataccactt atggaactgt tttggcagta ctaagagagc
     3541 cgttaccagc tagccattat atgattgcgg tagctggtaa agatgtccga gtagcagaat
     3601 atgccacata tgggactaag gaattggctg aaaatgctgc ggaggctatg aaagacagac
     3661 gtgcagtctt tcttgccaac cacggtatct tggctggtgc tcaagactta ctaaatgcct
     3721 tcaatatcat tgaggaggtt gaatactgtt caaaaattta ttgtgttgct aaaagcatgg
     3781 gtgaaccagt catcctccca gatgaagaaa tggaattgat ggctgaaaaa tttaaaactt
     3841 acggacaaag gaaataaaac ggaggaacat aaaatgaaaa aaggtttgtt gaaatgcgga
     3901 gcattgtgtt tatttgcttc agtgattcta gctgcttgcg gatcgtcatc aggaactagt
     3961 tcaagttctg aaaaagcagc aagtggtgat gatacagttt tagagttcta tcatggctat
     4021 caccatagcg aagaagaatg gccagctgct aaagttatgc gtgatcttta tgatgaattt
     4081 gcgaaaaaac atgcaaccgg tgatgttgaa tttaaaccaa ctcctgtgaa cggaagcttg
     4141 acagacatta tgaataacaa ggttgcaagt ggcgagtttc cagatatgat tgacttggct
     4201 ggtaacgatg tttcgctagc agctattgaa caaagtttgg ttctggattt gaaaccatat
     4261 atcgatgata atggattgga aaaaaatgtt ggtctaaact atacacaaaa tgatgtgaac
     4321 ggaaaaatct atacagtcca cgaccaatta ttaaccttag gtctttggta caatgcagat
     4381 attttctcaa aagctggggc taaaactcct gaccaatgga ccaagtggag tgattttact
     4441 gaagctatgg ccttggttcg taaacaagga gtatatgcct ttggtgcagg agagccggca
     4501 attcgtctct ttaacactgt acttggctca tctgaagctg gccgtaaact attagaaggt
     4561 cctctaactt ctgaaggaat tgattcaaaa gaatttgcag aagcacttaa attggttatg
     4621 acagaagtgc aagcaaacgg ttcagctaat gcaggcggcg atgccaacgt ttattcaact
     4681 gattttactg aaaataaatc agcagtcttc ttcaacggtg tatgggctgc aggtggtttg
     4741 aaagataatg cagctttgaa accaggattg tacgctggta atgttgcgat tagttcagct
     4801 ggtggcggta ttacaatttc aagtaaacta tctgaggaag aagaaaaact tgcattggaa
     4861 tttgtaaaat acatgaccag tgacgaggtt caaactacaa ttttcgaaaa agtgggagcg
     4921 aacccagcta gccaagcgat tgatactgct caaattgcaa gcggttctga cgacgctact
     4981 gtaaaactct taggtgaagc gacatctatg gcaaacaatg cagatgtaac tgttcctact
     5041 gttgtatctg tttggggtgg tgacgttcgt actgcaatta tcaacgcatt gaccgaaagt
     5101 gctgctgacg gtgtcaatat cgatcagaaa gtaaaagaaa cacaagacat cttgaaagcc
     5161 cttatcggct aaaataacag ggctaaggat gtattcctta gccctttttt agaaagtgag
     5221 tattttatgg caaagaaaac catgacctta caaaatagga aggcacagaa acaacgattt
     5281 atttttctat ttttactccc gacattagtt tgtttctttc tcttctactt ttattcggtg
     5341 gtaactattt ttgcaacatc ctttactaag tgggattata cgaatttaac cagcccagaa
     5401 tttctaggtt ttggcaatct ttttgccaac tataagtaca tttttagtga atatcctttc
     5461 tttttggaag cactgaaaaa ttcaattaca tgggcaatta taggtgtagt agttcaagtc
     5521 ccattagctt tatcagttgc tattgcctta tcaaaaaaat tggctggttg gaaactatct
     5581 cgaaaccttt atatcatacc tagtattatt tctagtgccg caatgggttt gatttttctt
     5641 caaatctaca atccaaatta tggagtagtt aaccaagtta tccgcttttt taaccctgaa
     5701 ttttcggatg cgattttatt gacacctggc ttgaatatta ttgcgatgac ttgtgcttat
     5761 attttctttg cagggacatc gacaataatg atattgggtc aaatcttcgc cattccacaa
     5821 gagatccatg aagcagcaat actagataac attacagggt ggagaagaga atggtatatc
     5881 accattccaa tgataaaaga aacattaaag acagtttcga ttatggccgc aacttcgggt
     5941 ttcctattgt ataacgaagt attcttccta acaaatggtg cagctggaac aaaaagtatc
     6001 agttttatca taagggagtt agcggtatct agttctcgta cacaatatgc cagagctaac
     6061 actataggtg tgattcagat tttaggcgga atgctaatca ttttgtgtat caatctgata
     6121 ttcaaagaag gtaaacgtag aaagtgaggt agagcatatg gacaatcgtt tgaatggcct
     6181 ttctaagtca gggaaattaa ttatttacat ctatatggct attacttgtt taatttcagt
     6241 gtttccaatt ttatggatat ttctatcctc attaaaagcg gatcctatga aaaatccagg
     6301 cattagtttg ccaactgatt taagttttga tggttatatc aaggttttta ctgagttaca
     6361 tgtattggac tatttttgga acagttttaa agttgtttcc atatctgtga ttatcagtat
     6421 tatcatgatt tccatgtcat cttatgttat tgctcggatg gatttccgtg ggaaaaagct
     6481 gataacagca atgttgtact ccactctatt tatccctgca acagctatga ctttccctgt
     6541 ctatcgtttg gtaaatgttt tggggatata taatacgcca gtagctctga tttttgtcta
     6601 ctcttgtagc ggtattgcca tgagtttctt tatcataaag aattactttg caataatacc
     6661 taaggagttg gaagaagctg ctgaaattga tggagcgagc tatgcccaga cattttggaa
     6721 agtaatgtta cctattgcta gacctggtat tttaacagca gcaattttag cttttataaa
     6781 taactggaat gaatactatt gggcatcaat gttgatcatc aataaagatg agctgacggt
     6841 tccagccttg cttggtcaat tcacaactag ctttaatact aactataatg gtctcttttc
     6901 agcaattgtt gtgattatct taccaccaat tttactattt gcatttgcaa gtaaatactt
     6961 tattgaagcc cttggaggag gagcagtaaa aggatgacaa aaaatagttt acaagaaatt
     7021 aacaaacgta cagagtggtt ccaaaaagac cgctttggaa tgttcattca ctgggggctc
     7081 tattcaatcc ctggaaaagg ggagtggatc cgcagtcatc aacaattatc cattgatgac
     7141 tatcagccat attttgaagc atttaatcct gtaaagtaca atccgagaga gtgggcgaag
     7201 gcagcaaaag cagcaggtat gaaatatatg gtcttgaccg ccaagcacca tgatggattt
     7261 tgcttgtttg actcagccta tactgactac aaatctacaa atacacaaat caggaaagac
     7321 ttagtagctg aatttgttga atcagtacga gaagaaggat taaaagtagg tctatacttc
     7381 agtctgatag actggtatca tccagattat ccgaaatatg gcgatatgat tcatcctatg
     7441 cgtgagaatg aagcctttaa agatgagaag attgactttg acaactactt gactttctta
     7501 cacaatcaag tggaagaaat cgtaaccaag tatggcaaac tggatattct atggtttgac
     7561 tattcttacg gagaaatgtt cggcgagaaa tggcgtgcaa cagatttgat taagcttgta
     7621 cgtaagcatc aaccagatgt agttgttgac aatcgtttag aaacatcagg agaaggattt
     7681 ggcagtattg taacggataa tatcaatctt tattctggag actttgtcag tccagagcag
     7741 attgttccgc atgagggaat tcgaaatata catggagagc caattccttg ggagctttgc
     7801 ttaactatga ataataactg ggcctacaat cctacggatt atctctacaa atcgtccagg
     7861 acacttattc gtaagttggt tgagtgtgta agtaaaaatg ggaatatgat tctgaatgta
     7921 ggtccagatg cactaggaat tatcaaccaa gagagtctgg aaattctaaa tgattttgga
     7981 aaatggctgg ataagaatgg ggagtccatt tatggttgtg gcgttagttc attacctaag
     8041 ccagaatggg gctactatac tcaaaaaggg aatacggttt atgcccatgt cttcgaacag
     8101 ccaattggtc cacttgcctt gattggtatc gatgaaagca aagtgaaaag gatgagtttc
     8161 cttcatgatg gtagtgaagt gaagatttct aagagctgga caactaatgc ttacgaaggg
     8221 atttgttttg cccagtacgg cgatattcct cactttactt atcctttgcc agacagcgta
     8281 gatagtgtga ttaagattga attagtaggt gatgatagtg aagattgaaa ataaacagat
     8341 agcacgctat tttaaattag aagatgggaa gttttatact tcccatatct tgaataaaaa
     8401 gcttgatgaa cccattggaa atgaaaaaaa tacggaattc cttgtttctt tctgtgatgg
     8461 aagccagctc agctctgagg attttacagc tcatgtggtc gaacaaacca atgaaagtct
     8521 tttggttagt ttctatcatt cagaaatcca gttaaccatt ttttattcaa aacgtgaaga
     8581 tgtacttgct aaagaggtta ctattctgtc gtcttctaaa acaattaact acattgatgt
     8641 ggagcagttt ggttttgcaa acttagagca ggtatatatt ccgaaacagc aagaagatat
     8701 taaggaaatg gctggatttt ctggctatta tgtagagttg ggacaaccaa tttatgccaa
     8761 gtccttcttc tttggtatgg agtttccaat gggagaaaat agactggtag atcaaaccta
     8821 tttttctcgc tactatgtcg gtcacaaaat taaagagcct aaaaaaatct ggccagttgt
     8881 tattggtgcg gcttctggat ttgagaaagc aagcattcaa caggaattct ttacttacat
     8941 tagtggaatt gctcagccaa gttacttcag aaaacaatat aattcttggt atgaccatat
     9001 gactgccatt gatgaaggga ttattgtaaa gagttttgaa gagatttcac atggctttga
     9061 agacaatggt gtgaagttgg atgcctatgt cgttgatgat ggctggtgtg attaccaaag
     9121 tgtttgggag tttaatgaaa agttccctaa cggattaaca aaggtaaaag ctctcgtaaa
     9181 taatttaggt gcaagtcttg gtctttggat tggaccacgt ggaggctata acggtacaga
     9241 agttatcatg agtgactggt tggaggcaca tccagaattc ggtagcaaga atgccttgtc
     9301 aaatgatgtc aatattggtg acttcaatta tttggaaggc atgaagcaaa aaatgttgga
     9361 ttaccagaaa aagtatgata tcagctattg gaaaattgat ggttggttgc tgaagccaga
     9421 caaaccagac cctagtggcg agtttgccat gcacactatg actcctgtct atgaattctt
     9481 gattgacctc ttaaaagatt taagggcaga gcgaggagat agagattgct ggttgaactt
     9541 gacttcttat gtcaatccaa gcccatggtt cttacaatgg gtcaacagcc tttggataca
     9601 aatttcgcag gatgttggct ttacagaaaa tgctgggaat gatattcaac gaatgattac
     9661 ctaccgtgat tgccaatatg aagaattttt agctaaacgg gatattcaat tgccactttg
     9721 gagtctttat aaccatgaac ctgtctatgc gagcacggct catacctggt acatggacca
     9781 tcaaatgtat gcaagtgtag aagagtttga agattatctc ctatttattt ccactcgagg
     9841 aaatgctttt tgggagtttc attattccta tacaatgttt gatcaggaac gttggaaggc
     9901 caatgctcgt gctgtcaaat ggatagagaa caattactca actttaaaac atagtcaaat
     9961 gattggtggc aaaccgtcag agtttgaaat ttatggttac agatgtattg atgaagacgt
    10021 aggaaaagaa attctttcgc ttcggaaccc agctagtcat tcagcgacta ttcagttaga
    10081 taatatcaat ctttccgact atcaattggt tagaggagat tataaagctc tgacagaaac
    10141 agagtatgaa ctagctccat atacaattct gattgcagag aaactaggag ggtaatatgg
    10201 cagttaaata tgctttaggt gtagatattg gtggtacaaa agttgctgtg ggactggtag
    10261 atggtacagg tcatgtggct tatagtttaa aagtgccaag caataaagaa tcatcagaaa
    10321 ccttatttca atgcgtttgt acaactattc gagaactact aaacagtcag caactgaata
    10381 ttgaggatgt tgcagggatt ggagttggct tgccgggaaa agttgatgtt gaaaatggag
    10441 ttgcagtttt tcaaaacaac attccatggg agaattttcc tgttgtgaag cgacttcaaa
    10501 aagaatttgg aaatattcca ataaaaattg acaatgatgt gaaagtagcg gcctatgcag
    10561 agtatcgaat gctttcatta gaagcagatg atatgtttgg ctatatcact atctctacag
    10621 gtatcgctgc taccaatatc ataaataaca caattctaag aggctctggc tttgcgggcg
    10681 aaatcggttt tatgccagtt cggagtttcg gaagaacaag tggtctagaa atctcctgct
    10741 caggaccagc cattgaacgc caagggaagc aaatgtatgg tgaagatttg tcaaccaagg
    10801 cagtgtttga taattggcgt aaaggagata ttacagcatc ggctattatc gctaatgcga
    10861 gagatggcat ggtccaagcg attcataata tgatttgttt actggatcct aaaataatcg
    10921 ttttgggtgg tagtgttgca caaaataacc cagattttat tgaaga
//
LOCUS       DBNFEG010000063        10915 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.063, whole genome shotgun sequence.
ACCESSION   DBNFEG010000063 DBNFEG010000000
VERSION     DBNFEG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10915
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.063"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(72..1121)
                     /locus_tag="AC1PNG_001090"
     CDS             complement(72..1121)
                     /locus_tag="AC1PNG_001090"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HIP6701598.1"
                     /translation="MKKNRKLTLIASIVLGTLLIWFAVFVRLPYYLESPGGAADIRQV
                     LTVNNQIDQEDGSYNFTYVSVQQATALQLFAAQFDPYTTVRSSEEMTGGADNEEYFRI
                     ARFYMETSQNMAKYQGLTLAGKEVNLDFFGVYVLALTDDSTFKKVLNIADTVVSINGK
                     TFESSPDLVKYVSGLEVGSDVTVGYISAGQEKSADGKIIKLSNGKNGIGITLVDHTEV
                     QSSVPIDFQTGDIGGPSAGLMFTLAIYTQLAEPDLRDGRIIAGTGTIEQDGKVGDIGG
                     ADKKVISAAKSGASIFFVPNNPVDVEVLKKNPKAKTNYEEAKEAAEKAGLDIEVVPVK
                     TVQEAIDYLKKTKGE"
     gene            complement(1108..1596)
                     /gene="coaD"
                     /locus_tag="AC1PNG_001091"
     CDS             complement(1108..1596)
                     /gene="coaD"
                     /locus_tag="AC1PNG_001091"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIP6701599.1"
                     /translation="MSDKIGLFTGSFDPITNGHLDLIERASGLFDKLYVGVFTNPKKA
                     GLLTGLERKAILEKLFVGMENIEVVLSENELVVDVAKRYGVTHLVRGLRNATDLEYES
                     SFDFYNRQLAPDLETIYLIAKPELKFVSSSQVRELLYFKQDIGPYVPEIVSEEIRKNE
                     EK"
     gene            complement(1586..2125)
                     /gene="rsmD"
                     /locus_tag="AC1PNG_001092"
     CDS             complement(1586..2125)
                     /gene="rsmD"
                     /locus_tag="AC1PNG_001092"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIP6701600.1"
                     /translation="MRIVSGSYGGRPLKTLDGKTTRPTSDKVRGAMFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAISRGMESAVLVERDRRAQAIIRENIAMTKEADKFQLLAMEARQ
                     ALNSVGGPFDLVFLDPPYAEQEIEDVITELCRRDLLADDVMVVCETDKAVSLPEEIAE
                     LGIWKEKLYGISKVTVYVR"
     gene            complement(2424..3146)
                     /locus_tag="AC1PNG_001093"
     CDS             complement(2424..3146)
                     /locus_tag="AC1PNG_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HIP6701601.1"
                     /translation="MEKKFEKLSLAEIGIISFMGLILLISLGASWYKNAQSETSDTKT
                     VQQVPNQKKTVDTLFQDAENAVIVLEGTHTEEKLAEAQSAVNKVTDATKKAALQKRID
                     QVKELVGQQTDAQTQAKLKAELVRAEAAQKVADEKKVSETGTSQSTDLAEASETSQES
                     YVVPNQNIYGNQSVSSTSQYTSSVASTSTSASVPPTSEGAPVVETPTVSETPAETTDS
                     TTSLVESVETVPTADESSPDNP"
     gene            complement(3203..3589)
                     /locus_tag="AC1PNG_001094"
     CDS             complement(3203..3589)
                     /locus_tag="AC1PNG_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIP6701602.1"
                     /translation="METFWVFLVFLAVLGAWMGFNYWRLRRAATVIDNAEFAEKIHGG
                     QLIDLRDPSEFNRKHILGARNIPYQQLRQSTAALRKDRPVLIYENDRGQLVTPAALHL
                     KKQGFNEIYILSYGLNGWNGKVKTTK"
     gene            complement(3589..3807)
                     /locus_tag="AC1PNG_001095"
     CDS             complement(3589..3807)
                     /locus_tag="AC1PNG_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HIP6701603.1"
                     /translation="MKRLYDVQQLLKRFGIIVYMGNRLYDIEMMQIELNRIYQAGVLD
                     RLEYMEAELVLRREHRLELEYQKSRENE"
     gene            complement(4107..4709)
                     /locus_tag="AC1PNG_001096"
     CDS             complement(4107..4709)
                     /locus_tag="AC1PNG_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIP6701604.1"
                     /translation="MRILVAEDQAMLRDALCQLLGFQDAVEAVLQAENGSQAIALLEK
                     EPVDVVLLDIEMPEKTGLDVLEWVRGQALPVKVVIMTTFKRPGYFERAVKADVDAYVL
                     KERSIADLMRTIETVLAGRKEYSPELMDILLTQRSPLTNLESQLLKLVAEGLSNKEIA
                     CQLHLSDGTVRNYMTSILSKLGAENRTAAVRIAQEKGWLV"
     gene            complement(4706..5800)
                     /locus_tag="AC1PNG_001097"
     CDS             complement(4706..5800)
                     /locus_tag="AC1PNG_001097"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIP6701605.1"
                     /translation="MIFEKRKVPLMFFVGMIFLYFPLYYAQYTPNPTAVVLATILFAL
                     VYCSLFYTDHKLYSMLAWFYLIGYIAVMSFWGNLQFSLFIFNLSNMLGWYYKENRPTY
                     RTVSYGLLLLAIILWALFGPVPFEMRAFALVIHFFGLALLIFSWIETKRAALQQRLQE
                     QNASINLLLAENERNRIGQDLHDTLGHVFAMLSVKAELVQTLLDHDQIDQAKKEVADL
                     QTLAKDSMQEVRQIVQSLKQHTVAEELQILQQMLDLAGVDLVVLGGEIAEQLSLPVQN
                     KLALVLRELANNLLKHSQASKCRLVFEKDQQELVVYYEDNGVGFAQLTGQELHTIKDR
                     LVTVKGSLEFISLAKPTRLSIRIPFEEVVE"
     gene            complement(5809..6540)
                     /locus_tag="AC1PNG_001098"
     CDS             complement(5809..6540)
                     /locus_tag="AC1PNG_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6701606.1"
                     /translation="MKALVQVEAVKLSRSLGVFLLSIGMPVIFFLIFSSTVQFDDATM
                     QKAFIQSYMLTMTGFSMSGFALFTFPMMLAEDRNNSWLTFIQHSPLPIWQYYLSKLVR
                     VLLCFVSSIAVVFAVGGLVKGVEMTAQEWVISALLLLVTSIVFLAIGLLLVQIQSEQT
                     MSVVANLLYFVLAIMGGSWMPVSLFPDWVQRICKLMPSYHVNQLVTTYAQEGDFLWKS
                     LLIVLGYAIIFLGIALVMNRKTEQQ"
     gene            complement(6537..7415)
                     /locus_tag="AC1PNG_001099"
     CDS             complement(6537..7415)
                     /locus_tag="AC1PNG_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6701607.1"
                     /translation="MIEVRALDKVIKGRTILSDISFEIKSGECVALIGPNGAGKTTLM
                     SCLLGDRKATKGQVLLDGLAPQAQSNKEKVAVLPQENAIPTDLKVKELLRFFQAIYKD
                     SLTDVEIDSLLCFSPEQKNQLAGKLSGGQKRLLAFVICLIGKPKLLFLDEPTAGMDTS
                     TRQRFWEIVHELKEKGVTIFYTSHYIEEVEHTAERILVLHQGRLLRDTTPFAMRSEEQ
                     EKEVTLPIAFAAVVESLAEINEISYKQDTVSFKTRAIEQVWASLQEAGCRISDLEVQN
                     KTLLNSLFDKTREEES"
     gene            complement(7670..8188)
                     /locus_tag="AC1PNG_001100"
     CDS             complement(7670..8188)
                     /locus_tag="AC1PNG_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HIP6701608.1"
                     /translation="MMKQKYYQSPAEIASFSPRPSLADSKAVLNQAVADLSVAHSILH
                     QVHWYMRGRGFMIWHPKMDEYMEEIDGYLDEMSERLITLGGAPFSTLKEFSENSQLKE
                     VPGDYNVTIEEQLVRVVEVFRYLAALFQKGFDVSDEEGDSVTNDIFNVAKASIEKHIW
                     MLQAELGQAPKL"
     gene            8393..8605
                     /locus_tag="AC1PNG_001101"
     CDS             8393..8605
                     /locus_tag="AC1PNG_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HIP6701609.1"
                     /translation="MSEEKFDAKLEQLSGSAKEVIGKLTGDKEIEVEGLVEKGIGKAK
                     ELVEDAKDGLEGAINGIKKAFDKEEK"
     gene            8775..9209
                     /locus_tag="AC1PNG_001102"
     CDS             8775..9209
                     /locus_tag="AC1PNG_001102"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HIP6701610.1"
                     /translation="MKKIVFVCLGNICRSPMAEFVMKDMTSQFHIESRATSNWEHGNP
                     IYSGTQKIFQKHKIPYDKTKTSQQISQADFEEFDVIIGMDSNNVRDLQKMAPAHAQNK
                     IFQFAEKSVPDPWYTGDFDETYEMVKKGCQDWLDRLQSFDQE"
     gene            9341..9763
                     /locus_tag="AC1PNG_001103"
     CDS             9341..9763
                     /locus_tag="AC1PNG_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701611.1"
                     /translation="MKEFKLPSIKWHDITSHFTRPKLEILSLIIILICALSVFTGRIA
                     SKQAMTFNNGALQYNGYVVANKMNGQGKLTFDNGDVYEGQFKNGIFHGQGTYTSASGW
                     VYTGQFKNGYADGKGKLTTEGQATYEGTFKQGIYQYEN"
     gene            9753..>10915
                     /locus_tag="AC1PNG_001104"
     CDS             9753..>10915
                     /locus_tag="AC1PNG_001104"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIP6701612.1"
                     /translation="MKIKWLSVIRVIGLVFVLLYHFFIKYFPGGFVGVDLFFTLSGYL
                     TTALLIDEFAKHKKIDIVSFFRRRFYRILPPLVLTILLVLPLALLVRNDFIANIGNQI
                     AAALGFMTNFFEILSGGSYENQFSPHLFVHTWTLAIEVQYYLVWAGLVWYLTRISKSV
                     GQLRGSIFLTSISLFSLSFLAMSISSFFVDNFSTIYYSSFTHTFPFFLGSVLATIAGI
                     GNTTTAFQQQVNTLSIKQAIGLFLGGLGVELLLLFTLQFSSIWTYLIGFLLSSLATAT
                     MIFAARVLHEKTEDVKEPAIILFFASISYGIYLFHWPLLIIFTQLTNATIAAGLSFVF
                     STILSTISFYILEPYIAGKVGSLFGLPIDLKPYKKWLLGSFACLTIVGLGICL"
BASE COUNT         3156 a         2570 c         2050 g         3139 t
ORIGIN      
        1 attcttctac tttaccattt aggagaaatt tctgacaatt ataatattat tctctagcaa
       61 aaaaggacga tttactcgcc cttcgttttc ttcaaataat caatagcctc ttgaacagtt
      121 ttcacaggaa ctacctcaat atctagacct gctttctcgg ctgcttcctt agcttcttca
      181 tagttcgttt tagcctttgg gtttttcttc aaaacttcta catcaactgg attgtttggt
      241 acaaaaaaaa tacttgcacc tgacttagct gccgaaataa ccttcttatc agccccacca
      301 atgtcaccta cttttccatc ctgctcgata gtccccgtac cagcaataat tcgaccatca
      361 cgtaagtcag gctctgcgag ttgagtatag atggcaagtg taaacatcag accagcactt
      421 ggaccaccaa tatctcccgt ttggaaatca attggaaccg aactttgaac ctctgtatgg
      481 tctacgagag tgatgcctat tccgtttttg ccattagata atttgataat cttaccgtcc
      541 gcagattttt cctgaccagc gctaatataa ccaactgtta catcacttcc aacctccaag
      601 ccactcacat atttaaccaa atcaggagaa gactcaaatg tttttccatt gatactcaca
      661 acagtgtcgg caatattaag aactttttta aaggttgaat catccgtcaa ggcaagcaca
      721 tagacaccaa agaaatcaag attgacttct tttcctgcca aggtcaatcc ttgatacttc
      781 gccatatttt gcgaagtttc catgtaaaac cgtgctatac ggaaatactc ctcattgtcg
      841 gcccctcctg tcatctcttc agaggaacga actgtcgtat agggatcaaa ctgagcagcg
      901 aatagttgta aagctgtagc ttgttggact gatacatagg tgaagttgta agagccatcc
      961 tcttggtcta tttgattgtt caccgttagt acctgacgaa tatcggcagc tccacctgga
     1021 ctttccagat aataaggcag acgaacaaaa acggcaaacc aaatcaaaag agttcctaaa
     1081 acaatagatg caattaaggt taacttccta tttttcttca ttctttctaa tctcctcact
     1141 aacaatttct ggaacataag gacctatgtc ctgtttgaaa tacagcaatt ctcgaacctg
     1201 gctggatgat acgaacttaa gctctggctt ggcgattaaa taaatagttt ccaaatctgg
     1261 cgccaactga cgattgtaaa aatcaaaact tgactcgtat tccaaatcgg tcgcattacg
     1321 caatcctcgg accaaatgtg ttactccata gcgcttagct acatcgacta ccaattcatt
     1381 ttcagacaga acgacctcga tattctccat tcctacaaaa agcttctcca aaatggcttt
     1441 tctctccaaa cctgtcaaga gtcctgcctt tttaggattg gtaaaaactc ccacatataa
     1501 tttatcaaat agtccactcg ctcgttcaat taagtccaag tgtccattgg taatggggtc
     1561 aaatgagccc gtaaacaatc caatcttatc tgacatatac tgttacctta ctaattccgt
     1621 agagtttctc tttccatatc cccagctctg ctatttcctc cggaagagag accgccttat
     1681 ccgtttcgca gactaccata acatcatctg ctagtaagtc acgccgacaa agttctgtta
     1741 tcacatcttc aatttcctgc tctgcataag gagggtccaa gaataccagg tcgaaaggac
     1801 ctcccacact gttcaaagcc tgtctagctt ccattgctag aagctgaaat ttatctgctt
     1861 ccttagtcat tgcaatattt tcacggataa tggcttgtgc tcgtcgatct cgctcaacca
     1921 gaactgctga ctccatgcca cgagatatag cctcgataga taaaccacca ctgccggcgt
     1981 acaagtcaag aactctacca ccctcaaaat agggaccaat catattgaac atggctccac
     2041 ggaccttatc cgatgtcggt ctggttgttt ttccatctaa cgttttcagt ggtcttccac
     2101 catagcttcc cgatacaatt ctcataaggt atattatagc aaaaaatgaa gctcgttgct
     2161 gaaaccaagt gcacttcctg ctttcctttt tgcaccaact ttttcatcct cctatcaaat
     2221 acactctcaa ccgctatagc aggaaatcat tctcacatta ttcaataaaa tgataaaaat
     2281 aaaaagcaag gaaattctgt tttacgcgaa attcctcact cttatcgtat gtatttaaac
     2341 tagatcgtta caagaaaaaa ttgatgttga aatacaaata gctatttttt ccagtatgct
     2401 aatacacccc gtagctaaaa agcctacgga ttatccggcg aactctcgtc agctgttgga
     2461 actgtttcca ctgactcaac caggctagtt gttgaatccg tcgtttccgc cggggtttca
     2521 gaaactgttg gagtttctac cacaggtgct ccttcactag tcggagggac tgaagcactt
     2581 gtactagttg acgccacgct gcttgtatac tggcttgttg aactcacact ttgatttcca
     2641 tatatatttt gattaggcac tacataggat tcttgtgagg tctcactagc ttcagcaagg
     2701 tccgttgact gactagtccc tgtttcagat acttttttct catcagctac tttttgggct
     2761 gcttccgccc tgaccaactc cgcttttagc ttagcctgtg tttgagcatc tgtctgctgt
     2821 cccactagct cttttacttg gtcaatccgt ttctgtaatg ctgctttttt agtcgcatcc
     2881 gtaaccttgt ttactgcaga ttgtgcctct gcaagtttct cttcagtatg tgttccttct
     2941 aatacaatga cagcattctc agcatcctga aataatgtat caaccgtttt tttctgattg
     3001 ggcacttgct gtactgtctt tgtatcactt gtttcgcttt gagcattttt ataccagcta
     3061 gcgcctaagc taatcaataa aataagtccc ataaaagaaa taatgccaat ttctgccaat
     3121 gacaactttt caaatttctt ttccatattt tttcccttat ccgtctcatt gcataagaaa
     3181 atactctagc ctactgacaa tgctacttag ttgtttttac cttaccattc catccgttta
     3241 gaccatagct taaaatatag atttcgttaa agccctgttt cttcagatga agagctgcag
     3301 gcgttaccaa ctgaccacga tcattctcat agattaagac aggtctgtcc ttacgcaaag
     3361 cagctgtaga ctggcgcaat tgctggtaag gaatatttct cgcacctaag atatgcttac
     3421 gattaaactc actcgggtct cgcaggtcaa tcaactgacc tccgtgtatc ttttctgcaa
     3481 attctgcatt atcaatcaca gtcgctgcac gacgcaatct ccagtaatta aaacccatcc
     3541 aggctcccaa tactgccaaa aatactaaaa atacccaaaa tgtttccatc actcattctc
     3601 ccttgatttc tgatactcta attccaaacg atgttcacgc ctcaaaacaa gctctgcttc
     3661 catatattcc aaccgatcta agacacctgc ctgatagatt ctatttaatt ctatctgcat
     3721 catttcaata tcgtagagac ggttgcccat atagacaatt ataccaaacc gtttgagtaa
     3781 ttgttgcaca tcataaagtc ttttcataaa aaaattgtag cagaatttta ggtgtttttc
     3841 aatataatac tcaatgaaaa tcaaaaacag actagctcca aaggtttggg gaacctttgg
     3901 aggttggaga tagagcgaac gtagttcgtt actacttacg cagatagaac cctgttacta
     3961 ttttgtttca aggtaacagg ctccactgga gcctttcact catcaaatca agtcaacaac
     4021 gtctgatttt gattttcgaa gagtataaaa actcagtcaa aaatcaggtc tataaaaaga
     4081 ttggacaaat gcccaatctc attcgtttat actaaccaac ccttttcctg cgcaattctg
     4141 acagcagccg tacgattttc agctcctaat ttggacagga ttgaggtcat atagttgcgg
     4201 actgtcccgt cagacaggtg gagctggcag gcgatttcct tattggacag cccctctgcc
     4261 accagcttga gcagctgact ttccaagttg gttaagggac tgcgttgggt taggagaatg
     4321 tccatcaact ctggtgaata ttcctttcgc cccgccaaga ccgtttcaat ggtccgcatg
     4381 aggtctgcta tggagcgttc cttgagcaca taggcatcca catctgcctt gaccgcccgc
     4441 tcgaaatagc ccggtcgctt gaaggtggtc atgatgacga ccttgacagg caaggcttgc
     4501 ccccgcaccc attccaagac atccaggccc gtcttttcag gcatttctat atctagtagc
     4561 accacatcta ctggctcttt ttccaaaaga gcaatggcct gactgccgtt ttctgcctgt
     4621 aaaacagcct caaccgcatc ttgaaagccc aagagttgac agagggcgtc ccgcagcatt
     4681 gcttgatctt ctgcgactaa gattctcatt ctaccacctc ctcaaacgga attcggatgg
     4741 acaggcgggt gggcttggct agagagataa attccaaact tcctttcacc gttaccaacc
     4801 tgtcctttat agtatgtaat tcctgaccag tcaactgggc aaagcccaca ccgttgtctt
     4861 cgtagtatac aaccaattct tgctggtctt tttcaaaaac caaacggcac ttgctagcct
     4921 ggctgtgctt gagcaggtta ttggccaact ctctcagcac cagagccagc ttgttctgga
     4981 cgggcaagct gagctgctct gctatttccc cgcccaagac aactaagtcc actcctgcca
     5041 ggtccaacat ctgctgtaaa atctggagtt cctctgccac ggtgtgctgc ttgagggact
     5101 ggacaatctg gcggacctcc tgcatggagt ccttggccaa ggtctgaagg tctgccacct
     5161 ccttcttggc ctggtcaatc tgatcatggt ctaaaagtgt ctggaccagc tcggccttga
     5221 cagacagcat ggcaaagaca tggcccagtg tatcgtgcaa gtcctgaccg atacggttgc
     5281 gttcattctc agccagaagg agattgattg aagcattctg ctcctgtagg cgttgctgaa
     5341 gggctgcccg tttggtctca atccagctga aaatcagcaa ggccaagccg aaaaagtgga
     5401 taacaagtgc aaaggctctc atctcaaaag gaacagggcc aaagagagcc cataggataa
     5461 tagccagcaa caaaagcccg taggacaccg tccgataggt cggtcgattc tctttataat
     5521 accagcccaa catattggac aggttaaaga tgaagaggga aaattgcaga ttgccccaaa
     5581 aactcatgac ggcgatatag ccaatcaggt aaaaccaagc cagcatggaa tagagcttgt
     5641 ggtcggtata aaagagggag caataaacca aggcaaagag aatagtcgct aggacaactg
     5701 ccgtcggatt gggagtatac tgggcgtaat aaagtggaaa atagagaaag atcatgccca
     5761 cgaagaacat gagcggtacc tttctttttt caaaaatcat agttcacctc actgttgctc
     5821 agtttttcta ttcataacta gagcaattcc taagaaaatt atggcatatc ctaggacaat
     5881 tagcaaggat ttccagagaa aatctccctc ttgagcatag gtggtcacca gctggttgac
     5941 atggtagctc ggcatgagtt tgcagatccg ctgtacccag tcaggaaaga gggaaacagg
     6001 catccaagag ccacccatga tggccagaac aaaataaagc aagttggcca ccaccgacat
     6061 ggtttgttcc gactgaattt gcaccaaaag caagccaata gctagaaata cgatagaggt
     6121 tactaaaagt aagagagccg aaattaccca ttcttgagct gtcatctcta cacccttaac
     6181 taaaccaccc accgcaaaaa caacggcaat agaagacaca aagcagagca agacccgcac
     6241 gagtttggaa agatagtatt gccagattgg cagaggcgaa tgctggataa aggtcaacca
     6301 actattgttt ctatcctctg ccagcatcat gggaaaggtg aaaagggcaa agcccgacat
     6361 ggaaaagcct gtcatggtca gcatgtagga ctggataaag gccttttgca tggtcgcatc
     6421 atcaaactgg accgtcgatg agaaaatcag gaaaaagatg acgggcatgc cgatagatag
     6481 gagaaaaact cccaaacttc tagataattt gaccgcttct acttgcacca atgctttcat
     6541 gattcttcct ccctcgtctt gtcaaatagg ctgttgagca aggttttgtt ctgaacctct
     6601 agatccgaaa tccgacaacc tgcctcctgc aagctagccc aaacctgctc aatagctcgg
     6661 gtcttaaagc taaccgtatc ttgtttgtag ctgatttcat tgatttcagc caagctctcc
     6721 accaccgccg caaaagcaat cggcaatgtc acttcttttt cttgctcctc actccgcata
     6781 gcaaatggcg tggtatctct tagtagccgc ccctggtgca agaccaaaat ccgctccgcc
     6841 gtatgctcca cttcctcgat atagtggctg gtataaaaaa tagtcacacc cttttccttc
     6901 aactcatgga caatctccca aaaacgctgg cgggtcgaag tatccatgcc cgccgtcggc
     6961 tcgtccaaga aaagcaactt gggcttgcca atcagacaga tgacgaaagc caagagccgc
     7021 ttctgaccac ccgatagctt gccggccagt tgattttttt gctctgggga aaagcagagc
     7081 agactgtcga tttccacatc tgtcagacta tccttgtaaa tagcttggaa aaagcggagt
     7141 aattccttga ccttcaggtc tgtcgggata gcattttctt gaggcaagac tgccaccttt
     7201 tccttattcg actgagcctg tggagccaga ccgtccaaca aaacctggcc tttcgtcgcc
     7261 tttctatcac ccaacaaaca agacataagc gtcgtttttc cagccccgtt tggaccaatc
     7321 aaagccacac attctcctga cttgatttca aaggaaatat cagacaaaat ggtccgtcct
     7381 ttgataacct tgtctagtgc tcgtacttct atcatttttc tacctcctac ttcttgaaca
     7441 tcttacatac atagtataac tagaaaaggg aagccccact agaagccttt gtcatggact
     7501 catgtgacaa atgtcatatt atactcaacg aaaatcaaaa gtagcctagg aaacgaagtc
     7561 gaagatagat ctggagttca tcaaggcaag ttgacaacgg ataattttga tattcgaaga
     7621 gtattaaata atgcagcaaa aaaagagtcc ttctggaccc ttcatctatt tacaacttag
     7681 gtgcttgacc aagttctgcc tgcagcatcc aaatatgttt ttcaatacta gccttagcca
     7741 cattgaaaat gtcgttggtt acgctgtcac cttcttcatc actgacatca aacccctttt
     7801 ggaaaagagc agccagatag cggaacacct cgaccacacg caccaattgc tcttcaatcg
     7861 ttacattgta atcaccagga acttccttga gctgactatt ttcactaaac tctttaaggg
     7921 tagaaaatgg tgccccacct aaggtgatta aacgctcact catctcatcc aaataaccat
     7981 caatttcttc catatattca tccatctttg gatgccaaat cataaagcca cgaccacgca
     8041 tataccagtg aacttgatgg aggattgaat gggctactga taaatcggcc accgcttgat
     8101 tcaaaacagc cttagaatca gccagtgatg gacgcgggct gaaagacgca atttctgctg
     8161 gagattgata atatttttgt ttcatcatat atcacccttt cttttattta taataattat
     8221 aaataaaaga agaaaaaatt gcaagaatta tccctcatcc tcctctggct tattgacaga
     8281 caaatccctt gctatattaa cttatatacg ttagactagt aagtaagaat agtatgtgat
     8341 tttcttttta gaaaatcatc gcccccatat gtgtatatcg aggagggatt ttatgtcaga
     8401 agaaaaattt gacgcaaaac tagaacagct atctggctct gcaaaagagg taattggtaa
     8461 gctgaccggc gacaaggaaa tcgaagttga aggacttgtt gagaaaggaa ttggcaaagc
     8521 aaaagagcta gtcgaagatg caaaagacgg gcttgaaggt gccattaacg gcatcaaaaa
     8581 agctttcgac aaagaagaga aataaacaaa gagttcaata taggtaggat tggtcgtgca
     8641 ctgtttgata atgaacatta cacttaattt ggtcttttac ctatagcccg aacaaaaata
     8701 tgcaagagag gggaactcag acatttacct gtcgagtctg ccttctcttt tcttatgcca
     8761 cgaaaggaga atctatgaaa aaaatcgttt ttgtctgtct tggaaatatt tgtcgtagcc
     8821 ctatggctga atttgtcatg aaagacatga caagtcaatt tcacattgaa agccgtgcca
     8881 cttcaaactg ggaacatggt aatcctatct actctggaac acagaaaatt ttccaaaagc
     8941 acaagattcc ctatgacaag accaaaacct ctcaacaaat ctctcaagca gactttgagg
     9001 aatttgatgt catcattggt atggacagca ataatgtccg agatttacag aaaatggcac
     9061 cagcacatgc ccaaaataag atttttcaat tcgcagaaaa atccgtacca gacccttggt
     9121 atactggaga ttttgacgaa acctacgaaa tggtaaagaa aggttgccaa gactggttgg
     9181 acaggttaca gtcatttgat caagaataag attttctatg taatattttt gtaatatttg
     9241 taaaaaaatg gtaaactatt tctgactgaa ctgataattt cttacagtat cctagaaaga
     9301 actgattagc atcggtcaaa tcctatgaaa gagtaaaacg atgaaagagt tcaaattacc
     9361 tagcataaaa tggcatgata taacaagtca tttcacccgt ccaaaacttg aaatcctctc
     9421 ccttattatc atcctcatct gtgccctctc tgtatttaca gggcgaattg ccagcaagca
     9481 agccatgact ttcaataatg gcgccctgca gtacaacggt tatgtggtcg caaataaaat
     9541 gaatggtcaa ggaaaattga cctttgataa tggagatgtc tacgaggggc aatttaaaaa
     9601 tggaattttc cacggtcaag gaacctacac atctgcaagt gggtgggttt ataccgggca
     9661 atttaaaaac ggctacgccg atggaaaagg aaaattgacc accgaaggac aagctaccta
     9721 cgaaggaact tttaaacagg ggatttatca atatgaaaat taaatggtta tccgttatac
     9781 gtgtgattgg attagtcttt gtcctactct atcatttctt tatcaaatat ttcccaggtg
     9841 gctttgttgg ggttgacctc ttctttaccc tgtctggata tttgacaacc gcccttttga
     9901 ttgatgagtt tgcaaaacat aaaaaaatag atattgtcag ctttttcaga agaaggttct
     9961 atcggattct cccccctctg gttttaacga ttcttttggt ccttccactt gctctgcttg
    10021 tccgcaatga tttcattgcc aatatcggca atcagatagc cgctgcgctc ggattcatga
    10081 caaacttctt tgaaattcta tcaggtggta gttacgaaaa tcaattttca cctcatctat
    10141 ttgtgcatac ttggacacta gccatagaag ttcagtatta ccttgtctgg gctggtttag
    10201 tttggtatct gactcgcatc tcaaaaagtg tggggcaact acgtggatca atttttctaa
    10261 catctataag cctcttctcg ctaagttttt tagcaatgtc tatctcaagt ttctttgtgg
    10321 ataatttctc aaccatttac tactcgagct tcacacatac cttcccattc ttcctcggaa
    10381 gcgttctcgc aaccatcgct ggtatcggaa atacaacaac tgccttccag caacaagtga
    10441 atacattgag tatcaaacaa gctataggtc tgtttcttgg aggactagga gtagaacttc
    10501 ttcttctctt tactcttcaa ttcagttcca tctggaccta tctaattggt tttcttctat
    10561 ccagtttagc tacagcaact atgatttttg ctgctcgggt acttcacgag aaaaccgaag
    10621 atgtcaaaga acctgcaatt atcctcttct ttgcaagtat ttcttatggt atctacctct
    10681 tccattggcc cttgctcatt atctttacac aactgacaaa tgccactatc gctgcgggat
    10741 tatcctttgt tttttctact atcctatcaa ctatttcctt ctatattcta gaaccatata
    10801 ttgctggtaa agtaggaagc ttgtttggat tacccataga tttgaaacca tataaaaaat
    10861 ggcttcttgg tagctttgct tgcttgacca tagttggctt aggtatctgt ctgtt
//
LOCUS       DBNFEG010000064        10697 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.064, whole genome shotgun sequence.
ACCESSION   DBNFEG010000064 DBNFEG010000000
VERSION     DBNFEG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10697
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.064"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1071
                     /gene="proB"
                     /locus_tag="AC1PNG_001105"
     CDS             <1..1071
                     /gene="proB"
                     /locus_tag="AC1PNG_001105"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIP6701613.1"
                     /translation="EKTIVFKVGTSSLTQENGSLDRIKIARITNQLAQLHQKGYQIVL
                     VTSGSIAAGFRRLGFDKRPTKIAEKQASAAVGQGLLIEEYTQNLMKDGIVSAQILLTQ
                     DDFADARRYQNASQALQVLLKQRAIPIINENDTIAIEEIKVGDNDTLSAQVASLLKAD
                     LLVLLTDVDGLYTANPNSDPTAQHLPEIKEITEDLFAMAAGAGSSNGTGGMTTKLQAA
                     QIATKSGVPVFICSSKEDTALLQAVTQANRGTLFLADDHAMNQRKQWMAFYARTDAAV
                     EVDAGAVDAMLHQGRSLLATGVKALEGDFEVGQVVEVYSQADHRLIGKGRVKLSSNDL
                     QDQLANGRAEGVFIHRNDWVSL"
     gene            1136..2374
                     /locus_tag="AC1PNG_001106"
     CDS             1136..2374
                     /locus_tag="AC1PNG_001106"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIP6701614.1"
                     /translation="MTTTQVLLDSLLVHKVSINLATTEQKNQALSAMADQLVAQTEAI
                     LAGNAIDMEHAQGKISQVMQDRLLLTEERIEAMADGIRALIGLPDPVGLVLEESTRAD
                     GLNICKKSIPFGLVGMIYESRPNVTSDAAALAIKSGNAVILRGGKEAFHSAQAIVTAL
                     KAGLEQAGLSPKVIELVQDTSRASATELMTAKGKIDLLVPRGGAGLIQAVVENATVPV
                     IETGTGICHVYVDKDADLDKALRIVVNAKTSRPSVCNAAEVLLVHEEIASQFLPRLEE
                     ALSGQVELRADSQAQALLNQARPAGEQDFDTEFLDYVLAVKVVSNVEEAISHIAQHST
                     GHSEAIVTENSQTAEHFTLYVDSAAVYVNASTRFTDGGEFGLGCELGISTQKMHARGP
                     MGLREMTTYKYIITGDGHIR"
     gene            2384..3169
                     /gene="proC"
                     /locus_tag="AC1PNG_001107"
     CDS             2384..3169
                     /gene="proC"
                     /locus_tag="AC1PNG_001107"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIP6701615.1"
                     /translation="MKIGFIGLGNMGAALAHAVNQLPETQLLLSNHNPAKAQALQEKV
                     GGQLFSNGEIAEQAEVIFLGVKPHLIQTVLSGLQDQISQNPSAIWISMAAGVTLDSLA
                     AFVSADKLIRIMPNTPVAIGQGMTTYSLVNQELAPLLEQILEKSGKVQQVPESLIDAA
                     TAIAGCGPAFVYQMIEALTDAGVENGLTAQDAKVLAAQTLAGTAQMVLTSDKHPAQLR
                     QEVTSPGGSTIAGVVALEKSGFRYAIIRAVAAALKKTRKLGKK"
     gene            complement(3192..3596)
                     /locus_tag="AC1PNG_001108"
     CDS             complement(3192..3596)
                     /locus_tag="AC1PNG_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIP6701616.1"
                     /translation="MAGRKISPQSLKNLYQSNKEANQLTKESIETALLFLLEKKELKQ
                     ISVSELVRKAGVSRNAFYRNYKSKEEILEDYYERTSNNLKKKWHDLQDKVQKDGVKQS
                     FADFVQEQKRKAEQSKALSNVSQWIKEKTKRD"
     gene            3723..4574
                     /locus_tag="AC1PNG_001109"
     CDS             3723..4574
                     /locus_tag="AC1PNG_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIP6701617.1"
                     /translation="MSKWKIVADSGCDYRQLANLAPDTEFISVPLTIQVGEQAFVDDA
                     NLDIDHMMEVMEASKSAAGSACPSPQAYQAAFEGAENIIVVTITGGLSGSFNAARVAR
                     DMYIEEHPNVNIHLIDSLSASGEMDLLVDEINRLIGAGLDFSQVVEAITHYREHSKLL
                     FVLAKVDNLVKNGRLSKLVGTVVGLLNIRMVGEASAEGKLELLQKARGHKKSVTAAFE
                     EMKKAGYDGGRIVMAHRNNAKFFQQFSELVKASFPTAVIDEVATSGLCSFYAEEGGLL
                     MGYEVKA"
     gene            complement(4673..5932)
                     /locus_tag="AC1PNG_001110"
     CDS             complement(4673..5932)
                     /locus_tag="AC1PNG_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIP6701618.1"
                     /translation="MKKYQVIIQDILTGIEEHRFKRGEKLPSIRQLREQYHCSKDTVQ
                     KAMLELKYQNKIYAVEKSGYYILEDRDFQDHTVELNPADFQELPYEDFRICLNESLIG
                     RENYLFNYYHQQEGLAELISSVQSLLMDYHVYTKKDQLVITAGSQQALYILTQMETLA
                     GKTEILIENPTYSRMIELIRHQGIPYQTIERDLDGIDLEELESIFQTGKIKFFYTIPR
                     LHNPLGSTYDIATKTAIVKLAKQYDVYIIEDDYLADFDSSHSLPLHYLDTDNRVIYIK
                     SFTPTLFPALRIGAISLPNQLRDTFIKHKSLIDYDTNLIMQKALSLYIDNGMFARNTQ
                     HLHHIYHAQWNKIKDCLEKYALNIPYRISKGSVTFQLSKGILSPSIQHMFEKCYYFSG
                     QKADFLQIFFEQDFADKLEQFVRYLNE"
     gene            6054..6788
                     /gene="yaaA"
                     /locus_tag="AC1PNG_001111"
     CDS             6054..6788
                     /gene="yaaA"
                     /locus_tag="AC1PNG_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HIP6701619.1"
                     /translation="MKIIIPNAKEVNTNLENASFYLLSDRSKPVLDAISQFDVKKMAA
                     FYKLNEAKAELEADRWYRIRTGQAKTYPAWQLYDGLMYRYMDRRGIDSKEENYLRDHV
                     RVATALYGLIHPFEFISPHRLDFQGSLKIGNQSLKQYWRPYYDQEVGDDELILSLASS
                     EFEQVFSPQIQKRLVKILFMEEKAGQLKVHSTISKKGRGRLLSWLAKNNIQELSDIQD
                     FKVDGFEYCTSESTANQLTFIRSIKM"
     gene            6893..8338
                     /locus_tag="AC1PNG_001112"
     CDS             6893..8338
                     /locus_tag="AC1PNG_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIP6701620.1"
                     /translation="MKKRSGRSKSSKFKLVNFALLGLYSITLCLFLVTMYRYNILDFR
                     YLNYIVTLLLVGVAVLAGLLMWRKKARIFPALLLVFSLVITSVGIYGMQEVVKFSTRL
                     NSNSTFSEYEMSILVPANSDITDVRQLTSILAPAEYDQDNITALLDDISKMESTQLAT
                     SPATSYLTAYQSMLNGESQAMVFNGVFTNILENEDPGFSSKVKKIYSFKVTQTVETAT
                     KQVSGDSFNIYISGIDAYGPISTVSRSDVNIIMTVNRATHKILLTTTPRDSYVAIADG
                     GQNQYDKLTHAGIYGVNASVHTLENLYGIDISNYVRLNFISFLQLIDLVGGIDVYNDQ
                     EFTSLHGNYHFPVGQVHLNSDQALGFVRERYSLTGGDNDRGKNQEKVIAALIKKMSTP
                     ENLKNYQAILSGLEGSIQTDLSLETIMSLVNTQLESGTQFTVESQALTGTGRSDLSSY
                     AMPGSQLYMMEINQDSLEQSKAAIQSVLVEK"
     gene            8356..9045
                     /locus_tag="AC1PNG_001113"
     CDS             8356..9045
                     /locus_tag="AC1PNG_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HIP6701621.1"
                     /translation="MNNQEVNAIEIDVLFLLKTIWRKKFLILLTAVLTAGLAFVYSSF
                     LVTPQYDSTTRIYVVSQNVEAGAGLTNQELQAGTYLAKDYREIILSQDVLTQVATELN
                     LKESLKEKISVSIPVDTRIVSISVRDADPNEAARIANSLRTFAVQKVVEVTKVSDVTT
                     LEEAVPAEEPTTPNTKRNILLGLLAGGILATGLVLVMEVLDDRVKRPQDIEEVMGLTL
                     LGIVPDSKKLK"
     gene            9055..9732
                     /locus_tag="AC1PNG_001114"
     CDS             9055..9732
                     /locus_tag="AC1PNG_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HIP6701622.1"
                     /translation="MAMLEIARTKREGINKTEEYFNAIRTNIQLSGADIKVVGITSVK
                     SNEGKSTTAASLAIAYARSGYKTVLVDADIRNSVMPGFFKPITKITGLTDYLAGTTDL
                     SQGLCDTDIPNLTVIESGKVSPNPTALLQSKNFENLLATLRRYYDYVIVDCPPLGLVI
                     DAAIIAQKCDAMVAVVEAGNVKCSSLKKVKEQLEQTGTPFLGVILNKYDITTEKYSEY
                     GNYGKKA"
     gene            9771..10502
                     /gene="cps4B"
                     /locus_tag="AC1PNG_001115"
     CDS             9771..10502
                     /gene="cps4B"
                     /locus_tag="AC1PNG_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HIP6701623.1"
                     /translation="MIDIHSHIIFGVDDGPKTIEESLSLISEAYRQGVRYIVATSHRR
                     KGMFETPEKIIMINFLQLKEAVAEVYPEIRLCYGAELYYSKDILSKLEKKKVPTLNGS
                     CYILLEFSTNTPWKEIQEAVNEMTLLGLTPVLAHIERYDALAFQSERVEELIDKGCYT
                     QVNSNHVLKPALIGERAKEFKKRTRYFLEQDLVHCVASDMHNLYSRPPFMREAYQLVK
                     KEYGEDRAKALFKKNPLLILKNQVQ"
BASE COUNT         3154 a         2036 c         2492 g         3015 t
ORIGIN      
        1 gaaaaaacaa ttgtatttaa ggtcgggaca agttctctga cgcaagaaaa tgggagtttg
       61 gaccgtatca aaatcgctcg gattaccaat cagctggcac agttgcacca gaagggttat
      121 cagattgtac tagtaacatc tggttcgatc gctgctggtt ttcggagatt gggctttgac
      181 aagcggccga ctaagattgc ggagaaacag gcttctgcgg cggttggtca gggcttgctg
      241 attgaggaat atacgcaaaa tctgatgaaa gatggcattg tgtcggctca gattttgctg
      301 acccaggatg attttgcgga tgctagacgt tatcagaatg cctctcaggc cttgcaggtc
      361 ttgctgaagc agcgtgctat tcctattatc aatgaaaatg ataccattgc cattgaggag
      421 attaaggtgg gggataacga tactttatct gcccaggtgg cttctctctt gaaagcagac
      481 ctattggtgc tcttgacgga tgtagacggg ctttatactg ccaatcccaa cagcgatccg
      541 actgcccagc acttgcctga aatcaaggaa attacggaag atttgtttgc catggcagca
      601 ggagcaggct cgtccaacgg aacaggtggc atgaccacca aattgcaggc agcccagatt
      661 gcgaccaagt caggcgtgcc cgtctttatc tgctcttcaa aagaggatac cgccctctta
      721 caggcggtca cccaagccaa tcgtggaacg ctctttttag cagatgacca tgccatgaac
      781 caacgcaagc agtggatggc cttctatgct cggacagacg cggcggttga agtggatgca
      841 ggagcggttg atgccatgtt gcatcagggg cgcagtttgt tagctactgg tgtcaaagcc
      901 ctcgaaggag actttgaagt gggccaggtc gtagaggttt atagccaagc agaccaccgt
      961 ttgattggta aaggacgggt caaactgtcc tccaatgact tgcaggacca gttggcaaat
     1021 ggcagagcag agggcgtgtt cattcaccgc aacgattggg tgagcttata gttgttcagt
     1081 ttgtctcgat tatgttgacg aacgagcaaa ctgaaagact aaataggaga aatttatgac
     1141 aacaacacaa gtattattag atagtttatt ggtccacaag gtttctatca acctagcgac
     1201 aacagaacag aaaaaccagg ccctatcagc aatggcagac cagttggttg ctcagactga
     1261 ggcaatccta gcaggcaatg ccatcgacat ggaacatgcc caaggcaaga ttagccaggt
     1321 gatgcaggac cgtttgctgc tgacagagga acggattgaa gccatggcag atggtattcg
     1381 ggccttgatt ggcttgccag atcctgtcgg attagtcttg gaagaatcta ctcgggcaga
     1441 cggcttgaac atttgcaaga aatccattcc ctttggtttg gtgggaatga tttacgaaag
     1501 ccgaccaaac gtgacctcag acgccgctgc cttggccatc aagagtggta atgcggtcat
     1561 tttgcgtgga ggaaaggaag cttttcattc ggcccaggct attgtcacag ccctcaaagc
     1621 tggtttggag caggctggtc tttcacctaa ggtcatcgag ctagtccaag ataccagtcg
     1681 tgcctctgcg acagagttga tgaccgcaaa aggcaagatt gacctcttgg tgccacgtgg
     1741 cggtgcagga ctcattcaag ccgtcgttga aaatgcgact gtgccagtta tagaaacagg
     1801 aacaggcatc tgccatgtct atgtggacaa ggatgcggac ttggacaagg ccttgcggat
     1861 tgtggtcaat gccaaaacca gtcgtccctc agtctgcaat gcggcggaag ttttattggt
     1921 tcatgaagaa attgccagcc aattcctacc tcgcttggaa gaagctcttt ctggtcaggt
     1981 agaattgcgt gccgacagtc aggcccaggc tcttctcaac caagccagac cggcaggtga
     2041 gcaggatttt gacacagagt ttctggatta tgtcttggcg gtcaaggtcg tttcaaatgt
     2101 agaagaggct atcagccaca tcgctcaaca ctcgacaggg cacagcgagg ctatcgtaac
     2161 ggaaaacagc cagacagcag agcatttcac actttatgtc gattccgcag cagtctatgt
     2221 caatgcctcg acccgcttca cagacggtgg cgagttcggt ctgggttgtg agctgggtat
     2281 ttccacccag aagatgcacg cccgtggtcc aatgggactg cgtgaaatga ccacttacaa
     2341 gtacatcatc acaggcgacg gccacattcg ttaggaggac aagatgaaga ttggatttat
     2401 cggactgggc aatatgggag cggcattggc tcatgcagtc aaccagctgc cagaaaccca
     2461 gctcctcctc agcaaccata acccagcaaa agcccaggct cttcaagaga aggtaggcgg
     2521 tcagcttttt tctaatgggg aaatagcaga gcaggccgag gtcattttcc ttggggtcaa
     2581 gcctcacctg attcagacag tcttgtcagg attgcaggac cagattagcc agaatccgtc
     2641 agctatttgg atttccatgg cagcgggtgt gacccttgac agcctggcag catttgtatc
     2701 ggcagacaag ctcatccgca tcatgcccaa cacaccagtc gctatcggcc aaggcatgac
     2761 gacctatagc ttggtcaatc aagagctcgc tccgctattg gaacaaatcc tagaaaaatc
     2821 aggcaaggtc caacaggtgc cagagagcct aatcgacgca gcaacggcta tcgcaggctg
     2881 cggaccagcc tttgtctatc agatgattga ggctttgacc gatgcaggag tggaaaacgg
     2941 gctcacggct caggatgcca aggtattggc ggctcagacc ctggcaggta ccgctcagat
     3001 ggtcttgacc agcgacaagc acccggccca gttgagacag gaagtgacct cgccaggagg
     3061 atcgaccatc gcaggtgttg tagccctaga aaaatcaggc ttccgctacg ccatcatcag
     3121 ggcagtcgcc gccgccctca aaaagactag aaaattaggc aaaaaataga agacagcaaa
     3181 aaacgggact tttaatcccg ttttgttttt tccttgatcc attggctgac gttggacagg
     3241 gccttgcttt gctctgcctt gcgtttttgt tcctggacaa aatctgcaaa gctttgcttg
     3301 acgccgtcct tttgaacctt gtcctgcaaa tcatgccatt tctttttaag gttgttagaa
     3361 gtccgttcgt aatagtcttc aaggatttct tccttggact tataattgcg atagaaagcg
     3421 ttgcgagaaa ctcccgcttt acggaccaat tcagataccg aaatttgctt gagctccttt
     3481 ttctctagga gaaacagcag agcagtttct attgattcct tagtcagttg attcgcttct
     3541 ttatttgatt gatagaggtt tttcaaagat tggggtgaaa ttttacgacc tgccatagtt
     3601 ttccttttcc gagtgtcaca tctgaaagaa aatgctttca gatagggtag tttaatgata
     3661 taattgtaag taaatagaga tttattgtca aataaaaaag taaaattgga aatgagaata
     3721 ttatgtcaaa atggaaaatt gttgcagatt cgggctgcga ctatcgtcaa ttagctaatc
     3781 tagctccgga cactgaattt attagtgtgc cacttaccat ccaagttgga gaacaagcat
     3841 ttgttgatga tgcaaacttg gatattgatc acatgatgga ggtgatggaa gcatctaagt
     3901 ctgcagcggg gtcggcgtgt ccaagtccgc aggcttatca ggcagctttt gagggagctg
     3961 agaacattat cgttgtgacg attacaggtg ggttatcggg tagttttaat gcggcacgag
     4021 tagctaggga tatgtatatc gaagagcatc cgaatgtcaa tatccatttg atagatagtt
     4081 tgtcagccag tggggaaatg gatttacttg ttgacgaaat caatcgctta attggtgcag
     4141 gactagattt ttcacaagta gtagaagcaa taactcacta tcgggaacac agtaagctcc
     4201 tctttgtttt agcgaaggtt gataatcttg ttaagaatgg aagactgagc aaattggtag
     4261 gcactgtcgt tggtcttctc aatatccgta tggttggtga ggcaagcgct gaaggaaaat
     4321 tagagttgct tcaaaaggcg cgtggtcata agaaatctgt gacagcagcc tttgaagaaa
     4381 tgaaaaaagc aggttatgat ggtggtcgaa ttgtcatggc ccaccgcaac aatgctaagt
     4441 tcttccaaca attctcagag ttggtaaaag caagttttcc aacggctgtt attgacgaag
     4501 ttgcaacatc aggtctatgc agtttttatg ctgaagaagg tggacttttg atgggctatg
     4561 aagtgaaagc gtgattcaca gagtaacaat tttgggatgt aatttccact atagaataat
     4621 ccccctcgtc ttctaagttc gagggggatt gtttgtatga gactattgga tttcattcat
     4681 tcaaatatct tacgaattgc tccagtttat ctgcaaaatc ttgttcaaag aagatctgta
     4741 agaaatcagc tttctgtccg ctgaaataat aacacttttc aaacatgtgt tggatgctag
     4801 gcgaaagaat ccccttgctt agctgaaagg tcacgctccc tttggaaatt cgatacggga
     4861 tgttcaaagc gtatttctct aaacaatctt ttattttatt ccactgagcg tgataaatgt
     4921 gatgaagatg ctgtgtgttc cgtgcaaaca taccgttatc aatgtagagt gagagagctt
     4981 tttgcatgat aagattggta tcgtagtcga ttagactctt atgtttgatg aaggtatcac
     5041 gtagctggtt aggaaggctg attgcaccga ttcggagggc aggaaagagt gtcggtgtaa
     5101 aagattttat atagatgacg cgattatctg tatcaagata gtgtaaaggt aggctatgac
     5161 tagagtcgaa atccgccaaa tagtcatcct caatgatgta gacatcgtat tgctttgcta
     5221 attttacgat ggctgttttt gttgctatat cataggttga accgagaggg ttgtgcaagc
     5281 gaggaattgt gtagaaaaac ttaatttttc cagtttggaa gatactttcc aattcttcta
     5341 ggtcaattcc atctaaatcc cgttcaattg tttgataggg gattccttga tgtcgaatga
     5401 gctctatcat tcgtgaatag gtagggttct ctatcaagat ttccgttttt ccagccaagg
     5461 tttccatttg tgtgagaata tatagagctt gttgactacc agctgtgata accagttggt
     5521 ctttttttgt atagacatga tagtccatta acagactttg aacggaggaa atcaattctg
     5581 ccaatccctc ttgctggtga tagtagttga ataggtaatt ttcccggcca ataagacttt
     5641 cgtttagaca aatccgaaaa tcttcatagg gtaattcttg aaagtctgca ggattgagct
     5701 ctacagtatg gtcttggaaa tctctatcct ctaagatata ataaccgctt ttttcgacag
     5761 cgtagatctt attttggtat tttaattcca acatagcctt ttggacagtg tctttgctac
     5821 aatgatattg ctcgcggagt tgacggatag aaggtaattt ctctccacgt ttgaatcgat
     5881 gttcctctat tccagtcaaa atatcttgga tgataacttg atattttttc atctaggtcc
     5941 cctttttata gactatgtta ctagctagta tatagaaaaa attgaagaaa gacaatatat
     6001 gaataatggg gttgaggttc aggaattaag ctactctatg gtataattaa gtgatgaaaa
     6061 taattatacc taatgcaaaa gaagtaaata caaatctaga gaatgcctcg ttttatctcc
     6121 tgtctgatcg aagcaagccg gtgctggatg ccataagtca atttgatgta aaaaagatgg
     6181 ctgcctttta taaattgaat gaagcaaagg ctgagttaga agctgaccgt tggtatcgaa
     6241 tcaggacagg tcaagcaaaa acctatccag cctggcagtt atatgatggt ctcatgtatc
     6301 gttatatgga taggcgaggt atagattcga aagaagaaaa ttatctacgt gaccacgttc
     6361 gtgtagcgac agccttatac ggattgattc atccttttga attcatttca cctcaccgct
     6421 tagattttca agggagctta aagataggca atcagtcttt gaaacagtac tggcgaccgt
     6481 attatgacca agaagttggt gatgatgaac tgattctctc actggcttcg tcagaatttg
     6541 agcaggtgtt ttctccacag attcagaaaa gattagttaa aattcttttc atggaagaaa
     6601 aagcaggtca gctaaaagtt cactcgacta tatcaaaaaa aggcagagga agattgctgt
     6661 cctggttggc taagaacaat attcaggaat tatcggacat tcaagatttt aaggtggatg
     6721 gctttgaata ttgtacttcc gaatcaacgg caaaccaact taccttcata cgatcaataa
     6781 aaatgtgaaa ttatgaaaaa gataacgttt tccagcgcta aaaagggtag aaaaatatta
     6841 atttctatga tataatggat gcgttatagg taaaagtcta ggaaggttgt ttatgaaaaa
     6901 gagaagcgga cgaagtaagt cgtccaagtt caaattggta aattttgcgc ttttgggact
     6961 ttattccatt actctatgtt tgttcttagt gaccatgtat cgctataaca tcctagattt
     7021 ccggtattta aactatattg tgacgctttt gctagtagga gtggcagtat tggctggatt
     7081 attgatgtgg cgtaagaaag cgcgcatatt tccagcgctc ttacttgttt tttcactggt
     7141 catcacgtct gttgggatct atggaatgca agaagttgta aaattttcaa cacgactaaa
     7201 ttcaaattcg acattttcag aatatgaaat gagtatcctt gtcccagcaa atagtgatat
     7261 tacggacgtt cgtcagctta ctagtatcct tgctccagcc gaatacgacc aagataacat
     7321 caccgcttta ttggatgaca tatccaaaat ggaatctact caactagcaa ctagccccgc
     7381 gacctcttac ctgacagcat atcaatctat gttgaatggc gagagtcaag cgatggtgtt
     7441 caacggagtt tttaccaata ttttagaaaa tgaagatcca ggcttttctt caaaagtgaa
     7501 aaaaatatat agtttcaaag tgactcagac tgttgaaaca gctactaagc aggtgagtgg
     7561 agatagcttt aatatctata ttagtggtat tgatgcttat ggaccgattt ctacggtctc
     7621 tcgttcagat gtcaatatca ttatgactgt caatcgtgcg acacataaga ttttattgac
     7681 aactactcca cgagattcat acgttgctat cgcagatggc gggcaaaatc aatacgataa
     7741 actaacacat gctggtatct acggtgtcaa tgcttctgtg cacaccttag aaaatctcta
     7801 tgggattgac attagcaatt atgtgcggtt gaacttcatt tccttccttc aattaatcga
     7861 cttggtgggt ggaattgatg tatataacga tcaagaattt acaagtttac atgggaatta
     7921 tcatttccct gttggacaag ttcatttaaa ctcagaccaa gcattaggct tcgttcgaga
     7981 gcgctactct ttaacaggag gtgacaatga ccgtggtaaa aaccaggaaa aagtgattgc
     8041 tgccttgatt aaaaagatga gtacgccaga gaatctaaaa aattaccagg caatcctatc
     8101 tggattggaa ggctcaattc aaacggattt gagcttagaa acgattatga gtttagtgaa
     8161 tacccaacta gaatcaggaa cacaatttac agtagagtca caagcattga caggaacagg
     8221 acgctcagac ttatcttctt atgcgatgcc tggatcacaa ctttatatga tggaaattaa
     8281 ccaagatagt ctggagcaat caaaggcagc gattcagtcc gtacttgttg aaaaataaag
     8341 attttaggag aaaatatgaa caatcaagaa gtaaatgcaa tcgaaatcga tgttttattc
     8401 ttactaaaaa caatttggag aaagaaattt ttaattctct taactgcagt gttgactgcg
     8461 gggttggcat ttgtctacag tagtttttta gtgacacctc aatatgactc cactacccgt
     8521 atctatgtag tgagtcaaaa tgttgaagcc ggtgcgggct tgactaacca agagttacaa
     8581 gcgggtacct atttggcaaa agactatcgg gaaattatcc tatcacaaga tgtattgaca
     8641 caagtagcaa cggaattgaa tctgaaagag agtttgaaag aaaaaatatc agtttctatt
     8701 cctgttgata ctcgtatcgt ttctatttct gtgcgtgatg cggatccaaa tgaagcggca
     8761 cgtattgcaa atagccttcg cacctttgca gtgcaaaagg ttgttgaggt caccaaggta
     8821 agcgatgtga cgacacttga agaagcagtc ccagcggaag aaccaaccac tccaaataca
     8881 aaacgaaata tcttgcttgg tttattagct ggaggtatct tggcaacagg tcttgtactg
     8941 gttatggagg ttttggatga ccgtgtaaaa cgtcctcagg acatcgaaga ggtaatggga
     9001 ttgacattgc taggtatagt accagattcg aagaaattaa aataggagaa caatatggcg
     9061 atgttagaaa ttgcacgtac aaaaagagag ggaataaata aaaccgagga gtatttcaat
     9121 gctatccgta ccaatattca gcttagcgga gcagatatta aggttgttgg tattacctct
     9181 gttaaatcga atgaaggtaa gagtacaact gcggctagtc tcgctattgc ctatgctcgt
     9241 tcaggttata agaccgtctt ggtggatgca gatatccgaa attcagtcat gcctggtttc
     9301 ttcaagccaa ttacaaagat tacaggtttg acggattacc tagcagggac aacagacttg
     9361 tctcaaggat tatgcgatac agatattcca aacttgaccg taattgagtc aggaaaggtt
     9421 tctcccaacc ctactgccct tttacaaagt aagaattttg aaaatctact tgcgactctt
     9481 cgtcgctatt atgattatgt tatcgttgac tgtccaccat taggactggt aattgatgca
     9541 gctatcattg cacaaaaatg tgatgcgatg gttgcagtag tagaagcagg caatgttaag
     9601 tgctcatctt tgaaaaaagt aaaagagcag ttggaacaaa caggcacacc gttcttaggc
     9661 gttatcttga acaaatatga tattaccact gagaagtata gtgaatacgg aaattacggc
     9721 aaaaaagcct aatttctcag ataacataag tttgataagt aggtattaat atgattgata
     9781 tccattcgca tatcatattt ggtgtggatg acggtcccaa aactattgaa gagagcctga
     9841 gtttgataag cgaagcttat cgtcaaggtg ttcgctatat cgtagcgaca tctcatagac
     9901 gaaaagggat gtttgaaaca ccagaaaaaa tcatcatgat taactttctt caacttaaag
     9961 aggcagtagc agaagtttat cctgaaatac gattgtgcta tggtgctgaa ttgtattata
    10021 gtaaagatat cttaagcaaa cttgaaaaaa agaaagtacc cacacttaat ggctcgtgct
    10081 atattctctt ggagttcagt acgaatactc cttggaaaga gattcaagaa gcagtgaacg
    10141 aaatgacgct acttgggcta actcccgtac ttgcccatat agagcgttat gatgctctgg
    10201 catttcagtc agagagagta gaagagctaa ttgacaaggg atgctacact caggtaaata
    10261 gtaaccatgt gttgaagcct gctttaattg gcgaacgagc aaaagaattt aaaaaacgta
    10321 ctcgatattt tttagagcag gatttagtac attgtgttgc tagcgatatg cataatttat
    10381 atagtagacc tccgtttatg agggaggcgt atcagcttgt aaaaaaagag tatggtgagg
    10441 atagagcgaa ggctttgttc aagaaaaatc ctttgttgat attgaaaaat caagtacagt
    10501 aacctcatag aaatagtgga ggagctatga atattgaaat aggatatcgc caaacgaaat
    10561 tggcattgtt tgatatgata gcagttgcga tttctgcaat cttaacaagt catataccaa
    10621 atgctgattt aaatcgttct ggaattttta tcataatgat ggttcattat tttgcatttt
    10681 ttatatctcg tatgccg
//
LOCUS       DBNFEG010000065        10665 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.065, whole genome shotgun sequence.
ACCESSION   DBNFEG010000065 DBNFEG010000000
VERSION     DBNFEG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10665
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.065"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1251
                     /locus_tag="AC1PNG_001116"
     CDS             <1..1251
                     /locus_tag="AC1PNG_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIP6701624.1"
                     /translation="NVQSVQKFAAKISNQRHLIALRDGMATAMPLIIVGSVFMLIADF
                     PIPAFSAWLSDIGISEFLWKATDATFGIAGLVVTFAVAYHLAKRYDVDRLSAGVLSIA
                     SFVLLTPVLSSDAGNGFPLKYLGTAGLFVGIIVALISTEIFSFFVKRNIVLKMPDTVP
                     PNVAKAFSAIIPGLFIISLWLMILILLKATGIDNIHALIADTVAKPLGLVTQTLPGIL
                     VVIFIQCFFWMFGVHGAQVTGPILEPLLLQNSEANRLAYQAGEELPHIIIYEFLYNFV
                     FSGGAGCVMALAILTFFLSRSQENKALGKLSIAPVSFQVAEPILFGFPTILNIKMAIP
                     FVLAPVVTTIVTYFAMDWGLVAKPIGAVVSWTTPPVIAGFLASGGRISGAVIQIVTIL
                     INMAIYYPFFRMDDKAKLEREQGK"
     gene            1710..3272
                     /locus_tag="AC1PNG_001117"
     CDS             1710..3272
                     /locus_tag="AC1PNG_001117"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIP6701625.1"
                     /translation="MRVIEFLDTTLRDGEQTPGVNFSIKEKVTIAKQLEKWGISAIEA
                     GFPAASPDSFEAVRQIAAAMTKTAVTGLARSVKSDIDACYEALKDAKYPQIHVFIATS
                     PIHREFKLQKTKEEILAQITEHVSYARERFEVVEFSPEDATRTELDYLLEVVQTAVDA
                     GATYINIPDTVGFTTPQHYGEIFRYLTTNVKSDREIIFSPHCHNDLGMAVANTLSAIK
                     NGAGRVEGTINGIGERAGNAALEEVAVALEIRKDFYDVTSPIVLKETLNTSELVSRFS
                     GIAIPRNKAVVGGNAFSHESGIHQDGVLKNPLTYEIITPELVGVKKNSLPLGKLSGRH
                     AFVEKLKELDLYFEEGDVASLFACFKKLADKKEKITDADIRALVAGTEISNRDGFQFK
                     DLRLDSQSDGSIQAQVTFINQDEEEVVVVESGKGSVEAVFNAIDQFFHQEISLERYHI
                     DAITDGIDAQARVLVAVENKATETIFNASGIDFDVVKASAIAYIHANVMVQKENSGQI
                     DKKLSEKDLPHI"
     gene            3281..4333
                     /gene="leuB"
                     /locus_tag="AC1PNG_001118"
     CDS             3281..4333
                     /gene="leuB"
                     /locus_tag="AC1PNG_001118"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIP6701626.1"
                     /translation="MTKKIVALAGDGIGPEIMEAGLAVLEAVAGQVGFDYEIEERAFG
                     GAGIDAAGHPLPNATLQACRQADAILLAAIGSPQYDDAAVRPEQGLLQLRKELGLFAN
                     IRPVKIFDSLKDYSPLKADRLDGVDLVMVRELIGGIYFGEHILETDQASDSNTYQAEE
                     IERVVRSAFDLAQKRQKKVTSIDKQNVLATSKLWRKVADEVAKDYPDVTLEHQLVDSA
                     AMLLITNPSRFDVLVTENLFGDILSDEASVLTGTLGVLPSASHAVAGPSLYEPIHGSA
                     PDIAGQGIANPVSMILSVAMMLEESFDLVQAGQTIRQAVEAVFAEGIFTKDLGGTAST
                     KEMTEAIIAQIEREEI"
     gene            4330..5721
                     /gene="leuC"
                     /locus_tag="AC1PNG_001119"
     CDS             4330..5721
                     /gene="leuC"
                     /locus_tag="AC1PNG_001119"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIP6701627.1"
                     /translation="MSGKSIFDKLWDRHVITGQEGQPQLLYVDQHYIHEVTSPQAFDG
                     LREAGRQVRRPELTFATFDHNVPTVDIHNIRDVISKAQMDALAKNIEDFGIPHAAHGS
                     EHQGIVHMVGPETGRTQPGKFIVCGDSHTATHGAFGAIAFGIGTSEVEHVLATQTIWQ
                     VKPKRLLVEFVGQAQRGVYAKDFILALIARYGVDCGLGHVVEFAGPVIDRLSMEERMT
                     ICNMSIEFGSKMGIMAPDQTTFDYLRGRECAPADFDAAVADWKELYSDADTVYDKHLV
                     LDVSDLAPMVTWGTTPAMGVSFDEIFPAIRDHNDERAYAYMDLAPGQKAADIPVGYVF
                     LGSCTNARLSDLQLAARIVKGRKIAEQVTAIVVPGSRPVKRAAEKLGLDKIFMEAGFE
                     WRDPGCSMCLGMNPDKVPAGVHCASTSNRNFEDRQGFGAKTHLCSPAMAAAAALYGRF
                     VDTRQLDILKEGV"
     gene            5721..6314
                     /gene="leuD"
                     /locus_tag="AC1PNG_001120"
     CDS             5721..6314
                     /gene="leuD"
                     /locus_tag="AC1PNG_001120"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIP6701628.1"
                     /translation="MHQFTTWTGTTVPLMNDNIDTDQLLPKQFLKLIDKKGFGKYLLY
                     AWRYLDDNYTDNPDFILNQPDYQGASILISGDNFGAGSSREHAAWALADYGFKVIIAG
                     SFGDIHYNNDLNNGILPIIQPKEVRDQLAQLAPDQEITVDLADQLIRTPFGEFPFDIE
                     QDWKHKLLNGLDDIGITLQYQDLITEYEGNRPSYWQE"
     gene            6377..7162
                     /locus_tag="AC1PNG_001121"
     CDS             6377..7162
                     /locus_tag="AC1PNG_001121"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIP6701629.1"
                     /translation="MTKQIHWDGALSQEGFDIIKGEGGVIVCPTKVGYIIMTADKAGL
                     ERKFDAKERKRNKPGVVLCGSMEELRELAQLTPEIDAFYQKHWDEDILLGCILPWKPE
                     AYAKLQAYGDGREELMTDVRGTSCFVIKFGVAGEQIAKEMWEKEGRMVYASSANPSGK
                     GNRGKVEGIGERIASKVDLIIEADDYVASIQPDKTIETRYEQGVMVSMVDKDGKLIPE
                     QGMDSRSISPCPVVIRKGLDIDKIMMHLSDHFNSWDYRQGEYY"
     gene            complement(7351..7965)
                     /gene="def"
                     /locus_tag="AC1PNG_001122"
     CDS             complement(7351..7965)
                     /gene="def"
                     /locus_tag="AC1PNG_001122"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIP6701630.1"
                     /translation="MSVIERLTKAAHLIDMDDIIREGHPTLRQVAEEVAFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMKLRGGVGLAAPQIDVSKRIIAVLVPNPEDEEGNPPAQ
                     AYSLQEVMYNPKIVAHSVQEAAMEGGEGCLSVDREVQGYVVRHARVTVDYMDKNGEKH
                     RIKLKGFNAIVVQHEIDHLNGIMFYDRIDPEHPFAVKEGMLVIE"
     gene            8095..9258
                     /locus_tag="AC1PNG_001123"
     CDS             8095..9258
                     /locus_tag="AC1PNG_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP6701631.1"
                     /translation="MKRILEKASLLALSTMLVSTFAVSPAIPQMIEHFAQQGIAAAQV
                     ENLITVTSFAIMVTLLANGLVVRFLSERNIIIAGLLLMAIGGALPMFLTAYPLILLAR
                     ILLGLGIGLINARAINIIGNFFTGQERVQMMGLRGSAEVLGSAGLTLLVGWLTQFGWQ
                     PAFLVYLFALVILALFLLFVPKEELLARMEVKVEENAPKVKLDKTMWMMGIYLAFLAF
                     FVINVNTFLTIRIPQIVLAKGIGTAQQASLILSLMQVMGIVAGTVFSSLVGRLKGWLL
                     AVSYVVFGLAVVGIAFADNLWVLGLGGMVSGFFYSIILTIVFSQVTDRAPKALLNAVM
                     TIVLMGCNIGGATSAILPTYLEKLNPTPTGAFGIYAIGCALISAGLIYKQLKK"
     gene            9295..9921
                     /locus_tag="AC1PNG_001124"
     CDS             9295..9921
                     /locus_tag="AC1PNG_001124"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6701632.1"
                     /translation="MLENNRLNELILRFPEDVQLIFKDMIPSYQLGYVDYVYQTDSYA
                     TQNRRIKNLAKNFRRMDYFYIMPLPQDLALEIANQWRYQPPLDAYTISANPETYAEMI
                     SPEARGDRFFAVIRNAALMGYFCIDQDGETVDIRLGMKPSLTGQGNGRAFYQAIEDYL
                     VEHVQPASIKLTVASSHQVAQKLFHALGFTEIEKQSEYIKMEKKLVCD"
     gene            9912..10640
                     /locus_tag="AC1PNG_001125"
     CDS             9912..10640
                     /locus_tag="AC1PNG_001125"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIP6701633.1"
                     /translation="MRLDKFLVDCGVGSRTEVKKLLKNKQVTVNGQVETSPKQQIDEK
                     SDQIAVAGQVLSHETFVYYVLNKPKGVISATEDDRHRTVLDLLDNTARQKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVMLSPKKHVDKLYRAQVDGIMTQEDVERFAAGIELKDLTC
                     QPAQLTILSTDEAKQTSLVDITIREGKFHQVKRMVQACGKTVTDLQRLTMGPLRLDPK
                     LALGEYRRLTAAELKQLEVFGVEL"
BASE COUNT         2787 a         2211 c         2663 g         3004 t
ORIGIN      
        1 aatgttcaat cggttcaaaa gtttgctgca aaaattagca accaaagaca tttaattgcc
       61 ttgcgtgacg ggatggctac tgcaatgcct ctcattattg ttggctcagt ttttatgtta
      121 attgcagatt ttccaattcc tgcgttttca gcctggcttt ctgatatagg gatttcagaa
      181 tttttatgga aagcaactga cgctactttt gggatagctg gcttagtagt aacatttgct
      241 gtcgcttatc atctagctaa acggtacgat gttgatagat tatctgcagg agtgctttct
      301 atagcgtcat ttgtgctttt aaccccagtt ttatcatccg atgcaggaaa tggttttcct
      361 ttgaaatatt taggaactgc tggcttattt gtaggcatca ttgttgcctt aatatccact
      421 gagatattca gtttctttgt taaaagaaac attgttttga aaatgcctga tactgtacca
      481 ccgaatgttg cgaaggcatt ttcagccatt attccaggtt tgtttattat ttcgctttgg
      541 ttgatgatac tgattcttct aaaagcaaca ggaattgata atattcatgc tttgatcgct
      601 gatactgttg cgaaaccact tggtctggtg acacagacac tgccgggaat attggttgtg
      661 atctttattc agtgtttctt ttggatgttt ggtgttcacg gggctcaagt cactggcccg
      721 attttggaac cacttttatt acaaaattca gaagctaacc gtctagccta tcaagcaggt
      781 gaagagttgc cacatatcat tatttatgaa tttctttata attttgtttt ttcaggtgga
      841 gcaggctgtg tgatggcatt ggcgatttta acgtttttcc tttctcgaag tcaagaaaat
      901 aaagctttag gaaaattatc aattgcgcca gttagtttcc aagtggcaga accgattcta
      961 tttggttttc caacaatttt gaatataaaa atggctatcc catttgtctt agcaccagtt
     1021 gtaacaacca ttgtcactta ctttgcaatg gattggggac tggttgcaaa accaattgga
     1081 gcggttgttt cgtggacaac gccaccagta attgcgggtt tcctagcctc aggtggtaga
     1141 attagtggag cggttattca aatcgttacg attttgatta acatggccat ttactatcca
     1201 ttcttccgaa tggatgataa ggcgaaattg gaacgcgaac aaggaaagta gtttgcttac
     1261 ttcctgacat aacaaagcga tagaaagaaa agcccttcgc aaggactatt tggttcaggt
     1321 gcgaaggact ttttttatta aggatgtgga atatatgaaa aatcaaatta agtgttgact
     1381 gcaaggctta aagagagttc ggatttttca gaaaatttcc atttttctgt tgacaagtga
     1441 aaataagtag ggtatcattg taaataatta aaatactgaa aggaaaaagt caaatgaaac
     1501 ctgtcactac aaaattcttt gctttgttgt tgcgacggtc gggctagtgc tgaaagaatg
     1561 cattagtcct gtttggctta ccaagcgggc actgttgaca acatctcgca ggtaatagtc
     1621 ctcgaggtgt tttctttttc ttagtatact ttgaaaacta agaaagagaa cctaatattg
     1681 acgttagggt tatttagaaa ggtattctta tgcgtgtgat tgaattttta gatacgactt
     1741 tacgagatgg tgaacagacg ccgggtgtga atttttcgat taaagaaaaa gtgaccatcg
     1801 ccaagcagtt ggaaaaatgg gggatttctg ccattgaagc gggtttccca gcggccagtc
     1861 ctgattcgtt tgaagcggtt cgtcagattg cggcagcgat gaccaagacg gctgtgacag
     1921 gtttggcacg gtcagtaaaa tcagatattg atgcttgtta tgaggctctg aaagatgcca
     1981 agtatccgca gatccatgtt ttcattgcga ccagccccat ccaccgtgaa tttaaactgc
     2041 aaaagaccaa agaagaaatt ttggcgcaaa tcactgaaca tgtcagctat gcacgtgagc
     2101 gttttgaggt ggtcgagttc tcgccagagg atgcgacgcg gactgagttg gattatttgc
     2161 ttgaagtggt gcagacagcg gtagatgctg gtgcgaccta catcaacatt ccagatacag
     2221 tcggctttac gactcctcag cactacggag aaattttccg ttacttgaca acaaacgtca
     2281 aatcggaccg tgagattatt ttcagtccgc attgccacaa tgacctaggt atggcagttg
     2341 ccaataccct atcggccatt aaaaacggtg ccggtcgtgt cgagggcacc atcaacggta
     2401 tcggtgagcg agcagggaat gcggcccttg aagaagtagc tgttgccctt gaaattcgga
     2461 aagatttcta tgatgtgact agtccgattg ttctaaagga aaccctcaac acttcagaat
     2521 tggtctctcg cttctctgga attgctattc cacgcaataa ggcggtggta ggtggcaatg
     2581 ccttctcaca tgaatctggt atccatcagg atggtgtctt gaaaaatcca ttgacttatg
     2641 aaatcattac acctgagttg gtaggggtca agaaaaattc tctgccactg ggtaaactat
     2701 ctggtcgcca tgcttttgta gaaaagctga aagaactaga cctgtatttt gaagaaggag
     2761 acgttgctag cttgtttgct tgtttcaaaa aactggcaga caagaaggaa aagattacgg
     2821 atgcggatat tcgtgccttg gtagcaggga cagaaatcag caatcgtgat ggtttccaat
     2881 ttaaagattt gcgtttggat agtcagtctg acggaagtat tcaggctcaa gttaccttca
     2941 tcaatcagga tgaggaagaa gtggttgttg tggagtcagg caagggttcg gttgaggctg
     3001 tctttaatgc cattgatcaa ttcttccacc aagagattag cttggaacgc taccatattg
     3061 acgccatcac cgatgggatt gatgcccagg cgcgtgtgct tgtcgctgta gaaaacaagg
     3121 caacggaaac aatctttaat gcctctggta ttgactttga cgtggtgaag gcttctgcca
     3181 ttgcctatat ccatgctaat gtcatggtgc aaaaagaaaa tagtggacag atagataaga
     3241 aactatcaga gaaggacttg ccacacatct aggagggaat atgacgaaaa aaattgtagc
     3301 cttggctggt gatggaatcg gtcctgaaat catggaggct ggcctagcag tcctcgaggc
     3361 tgttgctggg caagtaggat ttgattatga gattgaggag agagcttttg gtggcgctgg
     3421 gattgatgcg gctggccatc ctctaccaaa tgctacctta caagcctgcc gtcaggcgga
     3481 tgcgattttg ttagcagcta tcggtagccc tcagtatgat gatgcagctg ttcgaccaga
     3541 acaaggtctg cttcaacttc ggaaggaatt gggtctcttt gccaatatcc gtccggtgaa
     3601 aatttttgat agcctgaaag actattctcc tctgaaggcc gaccggctgg acggtgtgga
     3661 tttggtcatg gtacgggagt tgataggtgg gatttatttt ggagagcaca ttctggagac
     3721 agaccaagcc agcgatagca atacctatca ggcagaagag attgaacgag ttgtccgctc
     3781 tgcctttgac ctagcccaaa aaagacaaaa aaaagtcacc agcattgata agcagaatgt
     3841 actggcgacg tcaaaattat ggcggaaggt ggcggatgaa gtggcgaagg actacccaga
     3901 tgtgaccttg gagcaccagt tggtggatag cgcagccatg ctcttgatta ccaatcctag
     3961 tcgatttgac gtcttggtga cggaaaatct tttcggagat attttgtcgg atgaggccag
     4021 cgtattaaca ggaacactag gggttctgcc ttctgctagt catgcggtag caggtcccag
     4081 tctctacgaa cctatccatg gttcggcacc ggatattgcc ggtcagggca ttgccaatcc
     4141 tgtcagtatg attctatctg ttgccatgat gctggaagaa agttttgatt tggtccaagc
     4201 aggtcagacc attcgtcaag ctgttgaagc ggtctttgca gagggcattt tcaccaagga
     4261 tctcggtggg accgcttcta ccaaggagat gacggaggcc attattgccc agattgagag
     4321 ggaagagata tgagtggcaa atctattttt gataagctct gggaccgcca tgtgattaca
     4381 gggcaggaag gtcagcccca gctcctctat gtggaccagc actatattca tgaggtgacc
     4441 agtccccagg cttttgatgg tctgcgagag gcaggacgtc aggtccgtcg gccagaattg
     4501 acctttgcga cttttgacca caacgtacca accgttgata ttcacaatat ccgtgatgtc
     4561 atttccaagg ctcagatgga tgccttggct aagaatatag aagatttcgg cattccccac
     4621 gcagcccacg gttcggaaca tcaggggatt gtccacatgg ttggaccaga aacaggcagg
     4681 acccagccag gtaagttcat tgtctgcgga gatagccata cagcgactca cggagcattt
     4741 ggagctattg cctttgggat tggtacttct gaagtggagc acgttctcgc tacccagacc
     4801 atttggcagg tcaaacctaa gcgactctta gtagagtttg tcggtcaggc gcaaagaggt
     4861 gtctatgcca aggactttat cctggctctg attgcccgct acggcgtgga ttgtggtttg
     4921 ggtcatgtgg tcgagttcgc aggacccgtc atcgaccgac tcagcatgga agagcggatg
     4981 actatctgca atatgtccat tgagttcggc tctaagatgg gcattatggc tccagaccag
     5041 accacctttg actacctgcg aggtcgggag tgtgcaccag cggacttcga tgcggcggtg
     5101 gcagactgga aggagctcta ctcggatgcg gacaccgtct atgacaagca cctggtcctt
     5161 gatgtgtcag acttggcacc catggtcact tggggaacga cgcctgctat gggggttagc
     5221 tttgacgaaa tcttcccagc catccgtgac cacaacgacg agcgggccta tgcttacatg
     5281 gacttagctc ctgggcaaaa ggctgcggac attcccgttg gctatgtctt cctaggctcc
     5341 tgtaccaatg ctcgcctcag cgatttgcag ctggcagcta ggattgttaa ggggcggaaa
     5401 atagcagagc aggttacggc tattgtcgta ccaggcagtc gccctgtcaa gcgagctgca
     5461 gaaaagcttg gtctagacaa gatttttatg gaggctggtt ttgagtggcg ggatcctggt
     5521 tgctccatgt gtctgggcat gaatccagac aaggtccctg cgggtgtcca ctgcgcctct
     5581 acaagtaatc gaaactttga agaccgacag ggctttgggg ccaagaccca cttgtgtagt
     5641 cctgcgatgg ctgccgcagc tgcgctttat ggacgttttg tcgacaccag acagctagac
     5701 attctcaagg agggagtcta atgcaccaat ttaccacatg gacagggacg actgttccgc
     5761 tcatgaatga taatattgat accgaccagc tcttgcccaa gcagtttctc aagctgattg
     5821 acaaaaaggg ctttggcaag tatttgctat atgcttggcg gtacttggac gataactaca
     5881 cggacaatcc tgatttcatt ctcaaccagc ctgactatca aggcgctagt atcctcatat
     5941 ctggggataa cttcggagca ggttcttcta gggaacacgc tgcttgggct ctggcagatt
     6001 atggcttcaa ggttatcatt gcaggctcct ttggagatat tcattacaac aatgacttga
     6061 acaatggcat tctgcccatt atccagccca aagaagtcag ggaccaacta gctcagctgg
     6121 ctcctgacca agaaatcacg gttgacttgg cagatcagtt gatacggacg ccttttgggg
     6181 aattcccatt tgacatcgaa caagactgga aacacaagtt gctcaatggc ttagatgata
     6241 ttgggattac ccttcagtat caggatttga ttactgagta tgaggggaat cgcccaagct
     6301 actggcaaga ataaaataaa gattagaaaa caagcagatc gctctgctat ggaacaaaag
     6361 aaaagaggaa actattatga cgaaacaaat tcactgggat ggcgcacttt cacaagaagg
     6421 atttgacatt atcaaggggg agggaggcgt gattgtctgc ccgactaagg ttggttatat
     6481 tatcatgact gccgacaagg ctggtttgga gcgtaagttt gacgctaaag agcgtaagcg
     6541 taataagccg ggcgtagtcc tttgtggtag catggaggag cttcgtgagt tggctcagtt
     6601 gactcctgag attgatgcct tctaccaaaa acattgggat gaggacattt tgttggggtg
     6661 tatcctacct tggaaaccag aggcttatgc taagttgcaa gcctatggcg atggtcgtga
     6721 agagctgatg accgacgttc gtgggacttc ttgctttgtt atcaagtttg gtgtagctgg
     6781 cgagcagatt gccaaggaaa tgtgggaaaa agaaggtcgt atggtctatg cgtcatctgc
     6841 taacccgtca ggtaagggaa atcgtggtaa ggtagaaggt atcggtgagc gtatcgcttc
     6901 taaggtggat ttgattatcg aggcggatga ctatgtggca tctatccagc cagacaagac
     6961 cattgaaact cgctatgagc aaggggttat ggtgtctatg gtagacaaag atggtaaact
     7021 cattccagaa caaggcatgg atagccgttc tatcagccca tgcccagttg ttatccgtaa
     7081 gggtctggac attgataaaa tcatgatgca cttgtctgac catttcaact cttgggacta
     7141 cagacaaggg gaatattact aagatatcgt catttagttt atcttttatt acgtcgttaa
     7201 ctcaccttgc ctaactccag ttatgcctgc ggctcgttgc ctagtactaa aagtaaacta
     7261 aaagactatt attgagatcg atccgaaagg gtcgtttttt cgtactcaca taaaaaactg
     7321 gctgaaaatt tttcagtcag tttcttatgt ttattcaatt accaacatgc cttctttaac
     7381 ggcaaatggg tgttctgggt caatgcggtc gtagaacatg ataccgttga ggtggtcgat
     7441 ttcgtgttgg accacgatgg cattaaagcc tttgagtttg atacggtgct tttctccgtt
     7501 tttgtccatg taatcaacag tcacgcgcgc atggcggaca acgtaacctt ggacttcgcg
     7561 gtcaacggat aggcagccct cgcccccttc catagcagct tcttggacag aatgtgccac
     7621 gattttaggg ttgtacatga cctcttggag ggagtaggct tgggcaggtg gattgccttc
     7681 ctcatcttct ggatttggga ctaagacagc aatgatgcgt ttggaaacgt caatctgagg
     7741 tgcagctaga ccaactccgc cacggagttt cattttttca gccatgacag gatcctgcga
     7801 atgtttgagg aactgcatca ttttttcgcc taaaataatt tcctgatcag atagtggaaa
     7861 tgcaacttct tcggcaactt ggcgtagggt tggatgccct tcacggatga tgtcatccat
     7921 gtcaatcaaa tgggcagctt ttgtcaatcg ttcaataaca gacatgattc tctcctttga
     7981 atatagttaa gataagtata gcatgaaatg ggagagaata aaagtcggac ttttctatcc
     8041 attcgctttc aaatctgtta tactagtaaa agacgaacgt tgaaagggag aaaaatgaaa
     8101 agaattttag aaaaagccag tttgttggcc ctatccacca tgctggtttc gacatttgct
     8161 gtctcgccag ctattccaca gatgattgag cattttgccc agcagggaat tgcagcggcc
     8221 caggtggaaa acttaattac ggtgacctcc tttgctatta tggttactct attggcaaat
     8281 ggtctagttg tccgtttctt gtctgaacga aacattatca ttgcagggct tttgcttatg
     8341 gccatcggtg gagctttgcc catgtttcta actgcttatc ctctgatttt attggctcgc
     8401 atcttgcttg gtttggggat tggcttaatc aatgcgcgtg ccattaacat catcggtaat
     8461 tttttcaccg gtcaggagcg ggtacagatg atgggcttgc gtggttcagc agaggttttg
     8521 ggaagtgcag ggctcaccct ccttgtcggt tggttgaccc agtttggttg gcaacctgcc
     8581 ttcttggtct acctttttgc cttggtcatc ttggctctct tccttctctt tgttcccaag
     8641 gaggaattgc tggctcgcat ggaggtaaag gttgaggaga acgcgcccaa ggtcaaactg
     8701 gacaagacca tgtggatgat gggaatttat ctggcattct tagccttctt tgttatcaac
     8761 gtcaatacct ttctgaccat tcgtattcct cagattgttt tagccaaggg catcggtact
     8821 gctcagcaag ccagtctcat tctcagtctt atgcaggtca tggggattgt ggcagggacg
     8881 gtctttagta gcctggttgg tcgtttgaag ggttggcttt tagctgtttc ctacgtggtc
     8941 tttggactcg cggttgtcgg cattgccttt gcggataatc tctgggtgct gggactcggt
     9001 ggcatggtgt caggattttt ctacagcatc attctaacca ttgtctttag tcaggtaaca
     9061 gaccgtgcac ccaaggcctt gctcaatgcg gtcatgacaa tcgtccttat gggctgcaat
     9121 atcggcggtg cgacatctgc tatcttaccg acttatctgg aaaaactcaa cccaacccca
     9181 acaggtgcct ttggtattta cgccatcggc tgtgccttga taagcgcagg cttgatttat
     9241 aaacaactga agaagtagga gcggatttcc gctccttttt ggtataatat tcttatgtta
     9301 gaaaataacc gcttaaatga attaatacta cgttttcccg aggatgttca gttgattttt
     9361 aaggacatga ttccctccta tcagttgggc tatgtggatt atgtttatca gacagatagt
     9421 tacgccaccc aaaaccgtcg gattaaaaat ttggcaaaaa atttccgaag aatggattac
     9481 ttttacatca tgcccctgcc ccaggatctg gcattggaaa ttgctaacca atggcgctat
     9541 caaccacctt tggatgccta tacgattagt gcaaaccccg agacttatgc tgagatgatc
     9601 tcaccagagg cgcgtgggga ccgctttttt gcagtcatcc gcaatgccgc cctcatgggc
     9661 tatttctgta ttgaccaaga tggcgagacc gttgacattc gtttgggtat gaaaccttct
     9721 ttgacaggtc agggaaatgg cagagctttc tatcaggcta ttgaagacta tttagtagag
     9781 catgttcagc cagctagtat caaactgaca gttgctagct ctcatcaagt tgctcaaaaa
     9841 ctctttcatg ctcttggttt tacagaaata gaaaagcaat cagaatatat caaaatggaa
     9901 aagaaactcg tatgcgatta gataaatttt tagtggattg cggtgttggc agtcggacag
     9961 aagtcaagaa actcctgaaa aacaagcaag tcacagtcaa cggccaagta gaaacctccc
    10021 caaaacagca gattgatgaa aagtcagatc agattgcagt agcaggtcag gtcttgagtc
    10081 atgaaacctt tgtttattat gtattgaaca aacccaaggg agttatttca gcaaccgagg
    10141 atgaccgtca tcggactgtc ttggacctgc tagataatac agctcgccag aaggaagttt
    10201 ttccagttgg acgcttggac attgataccc atggcttgct cctcttaacc aacaacggtc
    10261 agctggctca tgtcatgctt tctccgaaaa aacatgttga taaactctac cgtgcacagg
    10321 tggacgggat catgacccaa gaggatgtcg aacgatttgc ggcaggaatt gagctgaaag
    10381 acttaacctg tcaaccagcc cagttgacca tcctctctac agatgaagct aagcagacct
    10441 cgctcgttga catcaccatc cgagaaggga aatttcacca ggtcaaacgc atggtgcagg
    10501 cctgtggaaa gacggtgaca gacttgcaac gattgaccat ggggcctctt cgcttagatc
    10561 caaaattagc gcttggagaa taccgcaggt tgactgctgc cgaattaaag caattagaag
    10621 tctttggtgt ggaattatag ataaatagaa gagacttgct cccga
//
LOCUS       DBNFEG010000066        10586 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.066, whole genome shotgun sequence.
ACCESSION   DBNFEG010000066 DBNFEG010000000
VERSION     DBNFEG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10586
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.066"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1310
                     /locus_tag="AC1PNG_001126"
     CDS             <1..1310
                     /locus_tag="AC1PNG_001126"
                     /inference="COORDINATES: protein motif:HMM:NF017691.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HIP6701634.1"
                     /translation="MFKKKATQIVSETSIKKSRKFLNRKTAGITGGILLTTFIGSQLM
                     TAELPKKDDLYGQQYVTVVKHLQEAGFKNIQGVELSDLEFGKIGESNLVELVSVDGED
                     WKEGRALKNIPITISYHVPKKDAVEFNLPASKNLADVEKELKDSGFKQVELTPVLLVE
                     EGNADKKDKIDRLQIGNHTYQSNHFYSTSLPVTLTYFDVSKDNIKLPENLAEAKTKPE
                     LEKQLKTAGFTDIKWTAVADKDKAKHEKIQKINLGGAEIQLPTKQEIISKKSTPIVIT
                     YYDFSSFAELPSSISTKTATDTKKLFTDGGFSQVSEVATETNEIAKNGQIIAVEIDGK
                     SFNEMNDTVIKKDSKVIIKYWNAEKAIAEKARKEEEERLAAEAQKVAEAQSQVQQFAA
                     TPSQNTYYPNCKAVRQAGAAPIYRGEPGYGSHLDRDGDGVGCE"
     gene            1372..1596
                     /locus_tag="AC1PNG_001127"
     CDS             1372..1596
                     /locus_tag="AC1PNG_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701635.1"
                     /translation="MAYKYRMILSFLLAGLCLYLVATVFAKSIWEGPLFLAFSFYSLI
                     YGCVMLYKWKPTAAKIIFQCIGEFLSLPWS"
     gene            1695..2276
                     /locus_tag="AC1PNG_001128"
     CDS             1695..2276
                     /locus_tag="AC1PNG_001128"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIP6701636.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     AFGVVSSRIGMRRDAVAIDDDMDIFGFIEKMEPRPYCVLIDEAQFLRRHHVYALARVV
                     DELDVPVMAFGLKNDFRNELFEGSQHLLLLADKLDEIKTICQYCSKKATMVLRTQDGK
                     PTYEGEQIQIGGNETYIPVCRKHYFSPEIKELP"
     gene            2557..3636
                     /gene="prfA"
                     /locus_tag="AC1PNG_001129"
     CDS             2557..3636
                     /gene="prfA"
                     /locus_tag="AC1PNG_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIP6701637.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEAATR
                     DTVTAYREYKTVLQNMVDAEEMIKESSGDADLEEMAKEELKQAKADKEAYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEGQGWRFEVMEASYNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLIMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVVDALVLYDQTQKLEELNK"
     gene            3636..4469
                     /gene="prmC"
                     /locus_tag="AC1PNG_001130"
     CDS             3636..4469
                     /gene="prmC"
                     /locus_tag="AC1PNG_001130"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIP6701638.1"
                     /translation="MNYAQLFAAYEEQLVAIGEEAESLSFTFRGLKGLNFTEFLLLLR
                     QEVTPTDKEEIDAIFQQLSQHRPAQYIIGKADFHGLEFAVDERVLIPRPETEELVNLI
                     LQENSGAGLRILDIGTGSGAIAISLAKARSDWEVVAVDISKDALAVAQENARTNQVSV
                     HFLESDVFQAVTGQFDLIVSNPPYISPDDTDEVGLNVLASEPHLALFAEEDGMAIYRQ
                     IAEQAGAFLKEKGKLYFEIGYKQGQDLTDLLALHFPKKRIRVLKDQFGQDRKVIADDN
                     G"
     gene            4456..5064
                     /locus_tag="AC1PNG_001131"
     CDS             4456..5064
                     /locus_tag="AC1PNG_001131"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIP6701639.1"
                     /translation="MTMDKLRTILENGGAVILPTETVYGLFAQALNEDAVNRVYQLKQ
                     RPRDKAMNLNVSNLNDIYFFSQNTPFFLEKLYNRFMPGPLTIILKANENVPFWVNSGL
                     DTVGFRLPNHEQTLRLISETGPLIGPSANISGNESGKKFSDIQAQFSVDLPGIEDDQA
                     LTGIDSTILDLSGQKARILRQGAISREEIQKAIPEIEFEVVL"
     gene            5061..5492
                     /locus_tag="AC1PNG_001132"
     CDS             5061..5492
                     /locus_tag="AC1PNG_001132"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6701640.1"
                     /translation="MIRPIEKGDLVVIREINAQSLGYDCSLEQTERQFFRCTSTLGHI
                     LLVYIDDISGAVQGYIHAQVYESLYSDTGLNILGLAVLPGHQVQGIGASLLKAVEQIA
                     QKEGYHFIRLNSAESRLQAHLFYEKNGYHSDKMQKRFIKHI"
     gene            5516..6775
                     /gene="glyA"
                     /locus_tag="AC1PNG_001133"
     CDS             5516..6775
                     /gene="glyA"
                     /locus_tag="AC1PNG_001133"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIP6701641.1"
                     /translation="MIFDKVNYKEFDKEVWEAIQAEEKRQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTECVDVVESLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMALIEPGDTVMGMDLAAGGHLTHGASVSFSGQTYNFVAYNVDEETGLLDYDAIL
                     KQAKEVQPKLIVAGASAYARTIDFAKFREIADAVGAKLMVDMAHIAGLVAAGLHPNPV
                     PHAHITTTTTHKTLRGPRGGLILTNDEELIKKINSAIFPGIQGGPLEHVIAAKAVSFK
                     EVLDPDFKDYAQKVIENSKAMAEVFLANPNFKVITGGTDNHLFLVDVTKVVENGKVAQ
                     HLLDEVNITLNKNSIPYEKLSPFKTSGIRIGSAAITARGFGVEEARKVAQLTIKALEN
                     AENEKALEEVRQEVRALTDQFPLYEGL"
     gene            6775..7749
                     /locus_tag="AC1PNG_001134"
     CDS             6775..7749
                     /locus_tag="AC1PNG_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HIP6701642.1"
                     /translation="MDIFVKKAIIHQFSPDDTELVLSDQLLTVSPKIEEYLRKKIERV
                     FSDEAKTGQFNPDNPFLDYLDGDLLTNSVKIANLWKEAFSISENLKTNDLIFIEFERN
                     GVEHFTFLRISLRENLAHVGLESGSPLKITQNNLPGAGSAPDEALIVNLQTRKYHLIE
                     KRIKHNGSFLNYFSDNLLQVTPAISAKKSIKAVEQTAQKIADNFHQGDFQFQSKIKSA
                     IFNNLEEDNELSPEKLADQLFDNNLTARLNFVDQLKEVIPDKISFDEIDSSRQLKKFE
                     NQKLSLSNGIELIVPNAIYEDAESVEFIQNDNGTYSILIKNIEDIKSK"
     gene            7750..8349
                     /locus_tag="AC1PNG_001135"
     CDS             7750..8349
                     /locus_tag="AC1PNG_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HIP6701643.1"
                     /translation="MKKLIRTLILLLLIFLGYKFYQTYSAVKQVMTYQTMVQEVLAEN
                     DTPANEELVLAMIYTETKGQEADVMQSSESATGYANTITDSRESIRQGIIYLTENLRL
                     AEEKGVEVWTAVQAYNFGPAYIDYIAEHGGEHTLSLAKEYSRTVVAPSLGNTTEETYT
                     YFHPLALLNGGKLYVNGGNIYYARQVRFNMHLMRLINLF"
     gene            8450..9208
                     /locus_tag="AC1PNG_001136"
     CDS             8450..9208
                     /locus_tag="AC1PNG_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIP6701644.1"
                     /translation="MKRNVLITGASSGIGKAIAYVFAANGDNIIITGRRLDKLEEIKQ
                     DIEKQYHVQVSVHSFDITNMEQTIVNCQKILEEVGQIHILVNNAGLALGLDKFQDYDL
                     TDMMTMIDTNIKGLLTVTRQLLPQMVENNEGHIINIGSTAGIYAYAGAAVYAATKSAV
                     KVLSDGIRIDTIDKNIKVTTLQPGIVETDFSQVRFHGDKERAATVYQGIDALQANDIA
                     SCTLFVANQPAHVQISDITIMATKQATGFTIHRD"
     gene            9225..9977
                     /locus_tag="AC1PNG_001137"
     CDS             9225..9977
                     /locus_tag="AC1PNG_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6287 domain-containing protein"
                     /protein_id="HIP6701645.1"
                     /translation="MKRKYLALALGLLTIMSLSACQTEKKGSSASTSTADTQVSNANT
                     STSSTIQREISLSSMDIDALMQGNYDSILGTWQNSQGNSLVFNSKGLVADSQSLLGRG
                     KITDGIFETGYVDATIGDVTLLMIPKGTQANTTETDTTDSTRDRMIVISQETTADVVD
                     VYYRLTNSATSGIDPLKNTETGVQLDSGPKTIDYANSILGENNWRVIEGNYTRTESIP
                     YNILEGDDNARYTIYQNGVIINADYQIVYQPQ"
     gene            9974..10306
                     /locus_tag="AC1PNG_001138"
     CDS             9974..10306
                     /locus_tag="AC1PNG_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HIP6701646.1"
                     /translation="MILNVQDFHLAAFAYYDGLDVIDQLKPGTPVQLVGEPSNPHDSE
                     AVAIFYQGTKLGYIPSDKNSLISRLIYFGHGDILEARIQMSNTENHPDRQFRVVVKLK
                     DNRKNDSR"
     gene            complement(10327..>10586)
                     /locus_tag="AC1PNG_001139"
     CDS             complement(10327..>10586)
                     /locus_tag="AC1PNG_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701647.1"
                     /translation="EKQLKISWKPSHSRKSRWRSSLVSVDKLSDRFFFQGELYKKGTY
                     PVFLEDLTIKNKAYQHRLDREFAAFLSIIEEQKRRAQLRVK"
BASE COUNT         3456 a         2066 c         2182 g         2882 t
ORIGIN      
        1 ttatgtttaa gaaaaaagca actcagatag ttagcgaaac gtcaattaaa aaatctagaa
       61 agtttcttaa tcggaaaaca gcaggaataa ctggtggcat cctacttaca acatttatcg
      121 gttcacaatt aatgactgct gagcttccaa aaaaagatga cctttatgga caacagtatg
      181 taacagtcgt aaaacatctt caggaagcag gttttaaaaa tattcagggg gtggaactta
      241 gtgatttaga atttggaaaa ataggtgaaa gcaatcttgt cgaactcgtc tctgttgacg
      301 gagaagattg gaaggaagga cgtgctttaa aaaacatccc aattacaatc tcctaccatg
      361 ttcccaaaaa agatgccgtt gaattcaacc taccagcttc taaaaattta gcagatgtcg
      421 aaaaagagct gaaagattca ggttttaagc aagtcgaact aactcctgtt ctgctagtag
      481 aagaaggcaa tgcggacaaa aaagataaaa ttgatcgcct tcaaatcgga aatcacacct
      541 atcaatccaa tcatttttac tctactagtc tccctgttac attaacctat tttgacgtta
      601 gcaaagataa catcaaacta ccagaaaatc tagcagaggc taaaacaaaa ccagagctcg
      661 aaaaacaact aaaaacagca ggatttacag acatcaagtg gactgctgtt gcagacaagg
      721 ataaagcaaa gcacgaaaaa attcaaaaaa ttaatctagg tggtgcagag attcaattgc
      781 ccaccaaaca ggagattatc agcaaaaaat caaccccaat tgtgattacc tactacgatt
      841 tctctagctt cgctgaactg ccatcatcca tttcaacgaa aacagctaca gatactaaaa
      901 aattgtttac cgatggtggt ttttctcaag tatctgaagt agcaacagag accaatgaaa
      961 ttgctaaaaa cggacaaatc attgcggtcg agattgatgg aaaatctttt aacgagatga
     1021 acgatacggt catcaaaaaa gatagcaaag tcattatcaa atattggaat gctgaaaaag
     1081 ctattgctga gaaagcacgt aaagaggagg aagaacgcct cgctgccgaa gcgcaaaaag
     1141 tagcagaagc acaaagtcaa gttcagcaat ttgctgctac accatctcaa aacacatatt
     1201 atccaaattg taaggcagtt agacaagcag gagctgcacc aatctatcgt ggagaaccag
     1261 gttacggtag tcatttagac cgtgatggag atggagttgg atgcgagtaa gaaaaaacat
     1321 tgacaacgtt tacattgcat gctatactgc tcttataagg agggattact catggcctat
     1381 aaataccgta tgattttatc gttcctactc gctggattat gtttgtatct agtagcaact
     1441 gtttttgcca agagtatctg ggaaggaccg ttgtttcttg cattttcttt ttacagcctg
     1501 atatatggtt gtgttatgct ctacaagtgg aaaccaacgg cagctaaaat cattttccaa
     1561 tgtattggag aatttctctc tttaccatgg tcttaatatc aaacaagctc aaaacgggct
     1621 tgtttttcta tttcctaaat ggtcaattta tgatagaatg gaaggagtga aaatttaaga
     1681 ttggaaagag aaaagtggct caattatatt ataaatatgg gacaatgaac tcaggaaaaa
     1741 ccattgagat tttgaaggtt gcccacaact atgaagaaca aggaaaacct gttgttatca
     1801 tgacatctgc acttgatact cgtgatgcct ttggtgttgt atccagtcgt attggcatga
     1861 gaagagatgc agtggcgatt gacgatgata tggatatctt tggttttatt gaaaaaatgg
     1921 aaccgcgtcc ttactgtgtt ctcattgatg aagcacaatt tcttcgacgt catcatgtct
     1981 acgcacttgc tcgcgtagtc gatgaattag atgtacccgt tatggctttc ggtcttaaaa
     2041 atgatttccg aaatgaacta tttgaaggtt cccaacattt gctcttattg gctgataaat
     2101 tagatgaaat caaaacaatc tgccaatatt gttctaaaaa agcgacaatg gttttgagaa
     2161 cacaggatgg aaaacctact tatgaaggag aacaaatcca aattggtggc aatgaaacct
     2221 acattcctgt ctgtcgcaaa cattattttt caccagaaat taaagaatta ccctaatttt
     2281 tgaaaatgaa atgagaagcg actgtaaatt gagcaactgt atagaactga atttgcctat
     2341 gactctgtgc caattttcat gacttacata ctacggcaaa ggaattgaac acgccctaca
     2401 agctaactag aagtgtgttc tgtcaaatat ttagaaagga attgaatacg ggctacgaac
     2461 tgtgcaaaaa agatagtttt tcctaggacg ctttcgtcct tcgtcaaaac tcctattttt
     2521 gctttgttcg tttaacgccc ttaatatctt aaaattatga acatctacga tcaattacag
     2581 gcggtggagg accgctacga ggaacttggt gaattgttgt ctgaccctga tgtagtcagc
     2641 gataccaagc gttttatgga gttgtctaag gaagaagctg caactcgtga tacagtaaca
     2701 gcctaccgcg aatataagac tgtacttcaa aacatggtcg atgcagaaga gatgattaaa
     2761 gaatcctctg gcgatgctga tttggaggaa atggctaagg aagaactcaa acaagctaag
     2821 gctgataaag aggcttacga agaaaagctg aaaatccttc ttttaccaaa agaccctaac
     2881 gatgacaaaa acattatctt ggaaatccgt ggtgccgcag gtggtgatga agccgccctc
     2941 tttgctggag accttttgac catgtatcaa aagtatgcag aaggtcaagg ctggcgtttt
     3001 gaagtcatgg aggcttctta taatggtgtc ggcggtatca aggaagtggt tgccatggtc
     3061 tctggtcaat ccgtttattc aaaactcaag tatgaatcag gagctcaccg tgtccaacgt
     3121 gtccctgtga cagaaagcca aggtcgtgtc cacacctcaa cagcaactgt cctcatcatg
     3181 ccagaagttg aagaagtgga atatgacatc gatccaaaag atcttcgtgt cgatatttac
     3241 cacgcttctg gtgctggtgg acagaacgtc aacaaggttg caactgccgt gcgtatcgtt
     3301 cacttgccaa ccaacatcaa ggtagaaatg caggaagaac ggacccagca gaaaaaccgt
     3361 gaaaaggcta tgaagattat ccgtgcccgt gtagctgacc actttgcaca gattgcccaa
     3421 gatgagcagg atgcagagcg taaatcaact atcggtacag gtgaccgctc agagcgtatc
     3481 cgtacttaca atttcccaca aaatcgtgtg acagaccacc gtatcggctt aaccttgcaa
     3541 aagctcgata ccatcttgtc aggtaagatg gacgaagtcg tagatgccct tgttctctac
     3601 gatcaaacgc aaaaactaga agagcttaac aaataatgaa ttacgctcaa ttatttgcag
     3661 catacgaaga acaattagtc gcaatcggtg aagaggcaga atccctgtcc ttcacctttc
     3721 gtggcttaaa gggattgaac tttacggaat ttctcctcct gctccgtcag gaagttacac
     3781 cgactgacaa agaagagatt gatgcgattt ttcagcaact ttcccaacac cgacctgccc
     3841 agtacattat cggcaaggct gattttcatg gcttggagtt tgcggtggat gagcgagttt
     3901 tgattccaag acctgaaacg gaagaattag ttaatttgat tttgcaggaa aatagcggag
     3961 cgggtttgcg tatcttagac atcggtacag gaagcggtgc catagctatt tcccttgcaa
     4021 aagctagatc tgattgggaa gtggtagcag ttgacatttc aaaggatgcc ctagcagtgg
     4081 ctcaggaaaa tgcaagaacc aaccaagtct ctgttcactt tctagaatcg gatgtcttcc
     4141 aagcagtgac agggcaattt gacctcattg tttctaatcc gccctatatc tcaccagatg
     4201 acacagacga agttggactc aatgtattgg cttctgagcc tcatctggcc ttatttgcgg
     4261 aagaagatgg catggctatc tatcgacaaa ttgcggagca agcaggggca tttttgaagg
     4321 aaaaaggaaa actctacttt gaaatcggct acaagcaagg gcaagaccta actgacttac
     4381 tcgctctgca tttccccaaa aagcgaatta gagtcctcaa agaccaattt ggacaggata
     4441 gaaaggtcat agcggatgac aatggataaa ctccgaacaa ttcttgaaaa tggtggtgct
     4501 gttatccttc caactgaaac agtctatggg ctttttgccc aagccttgaa tgaagatgca
     4561 gtcaatcgtg tctatcagtt gaaacagcgt ccgagggaca aggcaatgaa cttaaatgtc
     4621 tcaaatctga acgatattta ttttttcagt caaaatacac cattttttct ggagaaattg
     4681 tataatagat ttatgccagg accactaacg attatcctga aagcgaacga aaacgtccct
     4741 ttttgggtta attctggact ggatactgtt ggatttcgtc ttcccaatca cgaacaaacc
     4801 ttacgtttga tttcagagac tggcccactt attggaccgt ccgccaatat ttcaggaaac
     4861 gaaagtggca agaagttttc ggacatccag gcacaatttt cagttgattt gcctggcatt
     4921 gaggatgatc aagctctgac aggcattgat tcaacgattt tggacctttc tggacagaaa
     4981 gcgcggattt tgcgccaagg tgctatcagc cgtgaagaga ttcagaaagc cattcccgaa
     5041 atagaatttg aggtagtttt atgattcgtc ccattgagaa aggtgacttg gtagtcatcc
     5101 gagaaatcaa tgctcaatcc ttagggtatg attgttcatt ggaacaaact gagcgacaat
     5161 tttttagatg tacgagtaca ttggggcata tcttgttggt ctatatagat gacataagtg
     5221 gcgcagtgca aggttacatt cacgcacaag tctatgaaag tctctattcg gatacaggcc
     5281 tcaatattct cggactagct gttttgcctg gtcatcaagt acaagggatt ggagcaagcc
     5341 tgctgaaagc tgttgagcaa attgcacaga aagagggcta ccattttatt cgcctcaatt
     5401 ctgctgaaag tcgtctccaa gcccatcttt tctacgaaaa gaatggctat cactccgata
     5461 aaatgcagaa gcgttttatc aagcacattt agcccaatac aaaggagtac cgtatatgat
     5521 ttttgacaaa gttaactaca aagaatttga taaagaagtt tgggaagcta tccaagctga
     5581 agaaaaacgc caacaaaata atatcgaatt gattgcgtct gaaaacgttg tttctaaagc
     5641 tgttatggca gctcaaggtt ctattttgac caataaatac gcagaaggtt acccaggtcg
     5701 tcgttactat ggcggtacgg aatgcgttga cgtagttgaa agtttagcta ttgaacgtgc
     5761 taaagaaata ttcggtgcga aatttgctaa cgttcagcca cactcaggaa gccaggccaa
     5821 ttgtgctgcc tacatggcct tgattgagcc aggagacact gttatgggga tggacttagc
     5881 tgcaggtggt cacttgactc acggtgcatc agttagcttc tcaggacaaa cttacaactt
     5941 tgtagcttac aatgttgacg aagaaactgg cttgcttgat tacgatgcaa ttttaaaaca
     6001 agcaaaagaa gtacagccaa aattgattgt agcaggtgct tcagcctatg cacgtacaat
     6061 tgactttgct aaattccgcg aaattgcaga tgcagtgggt gccaaactga tggtggatat
     6121 ggctcatatt gctggtcttg tagcagcagg tcttcatccg aacccagtgc cacatgccca
     6181 tattacaaca actacgactc ataaaactct tcgtgggcca cgtggtggtt taattttgac
     6241 caatgatgaa gagcttatca agaaaatcaa ctcagccatc ttcccaggca tccaaggtgg
     6301 tccattagag catgttattg ctgcaaaagc cgtatctttc aaagaagttt tggacccaga
     6361 tttcaaagac tatgctcaaa aagtgattga aaacagcaag gccatggctg aggtcttcct
     6421 tgcaaaccct aactttaaag tcatcacagg aggaacagac aaccatctct tcctcgttga
     6481 cgtgaccaaa gttgttgaaa atggtaaagt tgctcagcat ctcttggatg aagtaaatat
     6541 tactctcaat aaaaactcaa ttccttacga aaaactctca ccattcaaaa caagcggtat
     6601 ccgtattggc tctgcggcca ttactgctcg cggttttgga gtagaggaag ctcgcaaagt
     6661 tgcacaacta actatcaaag cacttgaaaa tgcagaaaat gaaaaagctc ttgaggaagt
     6721 ccgtcaagaa gtgcgtgctt tgacagatca attcccactt tacgaaggtt tataatggat
     6781 atatttgtaa aaaaagccat catacatcaa tttagtcctg atgatacaga gctggtcctt
     6841 tccgaccagc ttttgactgt tagcccaaaa atagaagaat atttgcgtaa aaagattgaa
     6901 cgtgtctttt ctgatgaagc aaaaacagga caatttaacc ccgacaatcc ttttcttgac
     6961 tatttagatg gtgacctact gaccaactct gtcaagattg ctaatctatg gaaagaagca
     7021 ttttcaattt ctgagaattt aaaaaccaat gacctcattt tcatagagtt tgaacgaaat
     7081 ggtgtggaac attttacctt tttgagaatc tcactgcgtg aaaatctcgc ccacgttggt
     7141 ctggaaagcg gaagtccttt aaaaatcacc cagaataatt tacctggagc aggttcagca
     7201 cctgatgaag ccttgattgt caatcttcaa acgcggaagt atcatttgat tgagaaaaga
     7261 atcaaacaca atggatcctt cctcaattat ttctcagata atcttctgca agttacacca
     7321 gctatttctg caaaaaaatc aatcaaagca gtagagcaaa ctgcacagaa gattgctgac
     7381 aatttccatc aaggcgattt tcaatttcaa tcgaaaatca agtcagctat tttcaacaat
     7441 ttagaagaag ataatgagct ttcacccgaa aaattggctg atcagttatt cgataacaac
     7501 ctgaccgctc gtttaaattt cgtcgatcaa ctgaaagagg tcatacctga taagatttcc
     7561 ttcgatgaaa ttgatagtag ccgtcaactt aagaaatttg aaaatcagaa gctatcttta
     7621 tcaaacggaa ttgagttgat tgtgccaaat gcaatctatg aggatgcaga atcggttgag
     7681 tttattcaaa atgacaacgg aacctattct attcttatca aaaatattga agacataaag
     7741 agcaagtaaa tgaaaaaact gatacgtacg ctgatacttc ttttactgat ttttctcggt
     7801 tacaaattct atcaaaccta cagtgcagtc aagcaagtga tgacctatca aactatggta
     7861 caagaagtat tggcagaaaa cgacacacca gctaatgaag aactagtcct agcaatgatt
     7921 tacacagaaa ccaaaggaca agaagcagat gtcatgcaat caagtgagtc cgctactggc
     7981 tatgctaaca ctatcacgga tagtagagag agtatcaggc aaggaattat ctacctaaca
     8041 gaaaacctac ggttggctga ggaaaaagga gtagaggttt ggacagcagt tcaggcatat
     8101 aatttcggtc cagcctatat tgactatatt gctgagcatg gtggagaaca tacactctct
     8161 ttagctaaag aatactccag aacagtagta gcaccaagct tagggaatac tacagaagaa
     8221 acatatacct attttcaccc cttggctttg ctaaatggtg ggaagttata tgtaaatggt
     8281 ggaaatatct actatgctag acaggttcgt tttaatatgc atctgatgcg cctcattaat
     8341 ttattttaat acctatcaaa ctcatcggaa atctgatgag tttttaacta cagcattttt
     8401 acaaactagg taaaattgta gtataataaa gagaatagga gacagagtga tgaaacgaaa
     8461 tgtattgatt acaggtgcaa gtagtggtat tggcaaagca atcgcttatg tgtttgctgc
     8521 taatggtgat aatatcatca ttacaggacg aagattggac aaactagaag aaattaagca
     8581 agatattgaa aagcaatacc atgttcaggt atcagttcat tcatttgata ttacgaatat
     8641 ggaacaaaca attgtaaatt gtcaaaaaat cttagaagaa gttggacaaa ttcatatttt
     8701 ggtaaacaat gctggtctgg cattgggatt agacaaattc caagactatg atttgactga
     8761 catgatgaca atgattgata ccaatattaa aggattacta actgtgacac gtcagttatt
     8821 gccacaaatg gttgaaaaca atgagggtca tattatcaat attggctcaa cggccggtat
     8881 ttacgcctat gcaggagctg ctgtctatgc tgcaaccaaa tcagctgtca aggtgctttc
     8941 agacgggatc cgtattgaca cgattgataa aaatatcaag gtaactacgt tacaacccgg
     9001 tatcgttgag actgatttta gccaagtccg ttttcatgga gataaagaga gagcagcgac
     9061 agtctatcaa ggaattgatg ctcttcaagc aaatgatatt gccagctgta ctctatttgt
     9121 cgcaaatcaa cctgcacacg tgcagatttc tgatataacc attatggcaa ccaaacaggc
     9181 aactggcttt actattcata gagattagag gggagatagc tagaatgaaa agaaaatatt
     9241 tagcactcgc tcttggcttg ctcactatca tgtcgctttc tgcctgtcaa actgaaaaga
     9301 agggcagttc cgcaagtacc tcaactgctg atactcaggt ttctaatgcc aatacaagca
     9361 cgtcatcaac aatccaacgt gagatttctc ttagttctat ggatatagat gctctaatgc
     9421 aaggcaatta cgatagtata ctaggcacct ggcaaaatag ccaaggaaat tcactagtct
     9481 tcaatagtaa aggattagta gcagatagcc agtccttact tggccgcgga aaaataactg
     9541 acggaatttt tgaaacaggc tatgtagacg caacgattgg cgatgtaaca ctattaatga
     9601 ttcccaaagg aacacaagca aatacaactg agacagacac tactgactct acgcgcgata
     9661 gaatgattgt aataagccaa gaaacaacag cagacgtggt agatgtctat tatcgcttga
     9721 ccaattcagc cactagcggt atagacccat taaaaaatac tgaaacgggc gtccagttgg
     9781 atagtggacc aaaaacgatt gattatgcca actcaatttt aggcgaaaac aattggcgcg
     9841 tcattgaagg taactacacg cgcaccgaat ctattcctta taatattttg gaaggcgatg
     9901 ataatgcccg ttacactatt taccaaaacg gtgttattat caatgcagat tatcaaattg
     9961 tttatcaacc acaatgattc tcaatgttca agattttcat ttggcagcct ttgcctacta
    10021 tgatggacta gatgtaatcg accaactcaa gccaggaaca cctgttcagc tagtcggaga
    10081 gccttcaaac ccacatgact cagaagctgt agcaatcttt tatcagggca caaagttagg
    10141 atatatccca tcggataaaa actcactaat cagtcggtta atctattttg gacatggcga
    10201 tattctagaa gcacgtattc agatgtccaa tacagaaaat catcctgatc gccagtttcg
    10261 agtcgttgta aaactaaaag ataatcgtaa aaatgatagt cggtaattta ccaacctacc
    10321 cattttctac ttaacacgga gctgtgctct ccgtttttgt tcttcaataa tacttaaaaa
    10381 ggctgcaaac tccctatcca ggcgatgttg gtaggcctta ttttttattg tgagatcttc
    10441 caggaacact ggataggttc cttttttata caattctccc tgaaagaaga agcggtccga
    10501 taatttatca actgaaacaa gagaagaacg ccagcggctt ttacgagagt gagaaggttt
    10561 ccaagatatt tttaattgct tctcat
//
LOCUS       DBNFEG010000067        10553 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.067, whole genome shotgun sequence.
ACCESSION   DBNFEG010000067 DBNFEG010000000
VERSION     DBNFEG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10553
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.067"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            12..1007
                     /locus_tag="AC1PNG_001140"
     CDS             12..1007
                     /locus_tag="AC1PNG_001140"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIP6701648.1"
                     /translation="MAETKVMALREAINLAQSEEMRKDEKVFLMGEDVGIYGGDFGTS
                     VGMLDEFGPKRVRDTPISEAAIAGSAVGAAQTGLRPIVDLTFMDFITIALDAIVNQAA
                     KTNYMFGGGLKTPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGIKVVAPGTANDAKGL
                     LKSSILDNNPVIFLEPKALYGKKEEVNLDPDFYIPLGKGEIKREGTDVTIISYGRMLE
                     RALKAAEEVAAEGISVEVVDPRTLIPLDKELIIESVKKTGKVILVNDAYKTGGFIGEI
                     ASIITESEAFDYLDAPIIRIASDDVPVPYANILENAVLPNVEKIKAAIYKQVNKG"
     gene            1347..2735
                     /locus_tag="AC1PNG_001141"
     CDS             1347..2735
                     /locus_tag="AC1PNG_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HIP6701649.1"
                     /translation="MAIEIIMPKLGVDMQEGEIIEWKKQEGDFVNEGDVILEMMSDKT
                     SMELEAEESGVLLKIVHGNGATVPVTEVIAYIGAEGETVEAGASSAPAVEPAAAIEEV
                     PAGRTPVIVAPATAAKPQGSGKVRATPAARKLARELGIDLGLVPGTGANGRVHKVDVE
                     DFKGAAPKATPLAARIAADQGVDLSTLTGSGVNGKIVKNDVLAVLAPAAVETAAPAPK
                     AEEKPAKELPEGVEIIKMSPMRKAISKGMVNSYLTAPTFTLNYDIDMTNLMALRKQVL
                     EPIMNKTGLKVTFTDLIGLAVVRTLMKEEHRYMNASLINDAQEIELHKFVNLGIAVGL
                     DDGLVVPVVHGADKMSLSDFVVASKDVIKKAQSGKLKGAEMSGSTFSITNLGMFGTKT
                     FNPIINQPNSAILGVASTVQTPVVIDGEIKIRPIMALCLTIDHRIIDGMNGAKFMVDL
                     KNLLENPLELLI"
     gene            3009..4769
                     /gene="lpdA"
                     /locus_tag="AC1PNG_001142"
     CDS             3009..4769
                     /gene="lpdA"
                     /locus_tag="AC1PNG_001142"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIP6701650.1"
                     /translation="MAIEIIMPKLGVDMQEGEIIEWKKQEGDFVNEGDVILEMMSDKT
                     SMELEAEESGVLLKIVHGNGTTVPVTEVIAYLGAEGESVEVGSAPAPAEVAQATADLK
                     AAGLEVPAAPAAAPQAPKAELAADEYDMVVVGGGPAGYYAAIRGAQLGGKIAIVEKSE
                     FGGTCLNKGCIPTKTYLKNAEILDGLKIAAERGINLASTNYTVDMDKTVDFKNKVVKT
                     LTGGVQGLLKANKVTIFNGLGQVNPDKTVVIGDKVIKGRSIILATGSKVSRINIPGID
                     SKLVLTSDDILDLREIPKSLTVMGGGVVGVELGLVYASYGTEVTVVEMADRIIPGMDR
                     EVSVELQKVLSKKGMKFLTSVGVSEIVEANNQLTIKLNDGSEIISEKALLSIGRVPQL
                     AGLENLHLELDRGRIKVNAYQETSIPGIYAPGDVNGTKMLAHAAYRMGEVAAENAIHG
                     NHHKAKLDFTPAAVYTHPEIAMVGLTEDQAIEKYGKENILIGRNSFTGNGRAIASNEA
                     HGFVKVIADKKYHEILGVHIIGPVAAEMINEAATIMESELTVDDVAASIHGHPTFSEV
                     MYEAFLDVLGVAIHNPPKRK"
     gene            5005..5850
                     /locus_tag="AC1PNG_001143"
     CDS             5005..5850
                     /locus_tag="AC1PNG_001143"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIP6701651.1"
                     /translation="MRKIIFLDVDGTLVDYHNRIPESAIRAIRQARENGHLVYVCTGR
                     SRAEMQPELWEIGLDGMIGGNGSYVEHQGQVIMHQLLSEEDSRAIVDWLHERGLEFYL
                     ESNNGLFASENFRERARETLRIYSMNKGKTAEEVATQEVDDVIHGMIFDSQLYRNDLN
                     KVSFVLDSYQDHLDSKEAFPQLVANTWGGRGESALFGDLGVKDIDKAHAISVLLDHLG
                     ASQADTIAFGDAKIDISMLDYCAVGVAMGNGGAEILAMADMITDDVEEDGLYNAFERL
                     GLLDK"
     gene            6017..6850
                     /gene="licT"
                     /locus_tag="AC1PNG_001144"
     CDS             6017..6850
                     /gene="licT"
                     /locus_tag="AC1PNG_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HIP6701652.1"
                     /translation="MQIEKVYNNNVIQAIDQQGRELIVMGKGLGFQKKAGEELDTSKI
                     EKTFVLQNDYQQSDLSSLYLQMESGEVEVVNAIINRAEETLEVQFDLSLYLALADHLH
                     FVFQRCREGIFIENPLSWEVRKFYPKEYQIGLEALQLVQERLGLELGKGEASSIALHL
                     VNAQKNGAFGKETQTISKIVTQILDIVRLHFGSVTYEEDTSYHRFVTHIQYFAQRVAN
                     GVVEGVNDAFLYEQVMANYPDSFACTTKIRQHIAEVYHFEMSKDEQVYLTIHIQRLKT
                     S"
     gene            7050..8960
                     /locus_tag="AC1PNG_001145"
     CDS             7050..8960
                     /locus_tag="AC1PNG_001145"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HIP6701653.1"
                     /translation="MAKDYTDLATDIVAHVGGKENISSLKHCVTRLRFGLKDESKADT
                     DYLKARDGVVTVVQAGGQYQVVIGNHVPDVYAAVQKVAGISGDGALDIDEGDGPKGNL
                     FDRFVDLVSGVFQPSLGVLAAAGIIKGLVSILAATMGLNNDNSALYAILNTAGDGFFQ
                     FLPIVLAFNAARKFRMNEFTGMAIAMALVYPTLPGTFATLKEAGLNNVFGIPFVLPAA
                     GSYLQTVMPVLLAVWIASHIEKFMKKITPDVVKVFIVPFVTILISVPLTFLVVGPVAN
                     YASDLLSAGFTAVMGFSPLLYGILLGALWQVMVMFGLHWALVPLAILQFSQNGWSNIL
                     LAAALPNFTQTGVLLAIMMKTKESKVKSVAMPAFISSIFGVTEPAIYGVTLPMKTPFY
                     ISCAVSAVIGAGLSFFDLKNYAMGALGIFMYPGYVSPELGVTPMWIMMGFSLAAIVLS
                     FAIQMALPVPYLYGEPTEKKSVEETKESVPELKEIKQEIIASPLIGKVVKLEDVPDAV
                     FASGAMGKGIAIDPLDGILVSPAKAEVTLLFPTKHAIGLRTENGAELLIHIGMDTVSL
                     AGKGFESFVQVGDQVEAGQKLLEFDLHQIKAADLPVITPIIVTNTADYEDILVTQESQ
                     INSGDYLLTTVK"
     gene            complement(9005..9334)
                     /locus_tag="AC1PNG_001146"
     CDS             complement(9005..9334)
                     /locus_tag="AC1PNG_001146"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIP6701654.1"
                     /translation="MITMHLYYTGPADNARAFAQEMEASGIADRIRQQAGNTRYQYFA
                     PLDDPHSILLVDSWTDQAALDIHHASPMMQEILDLRSKYQLTVTAERYKSDEAGIPEQ
                     DKDFLQR"
     gene            complement(9584..10498)
                     /locus_tag="AC1PNG_001147"
     CDS             complement(9584..10498)
                     /locus_tag="AC1PNG_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6701655.1"
                     /translation="MQPPLTDKQFKHTIDYDSRLLPYKYYNTYVTDGIPDILFHWHNE
                     FEINYVYGGTARYHIDYDYFNSQAGDIILIRPNAAHSIHPIEQRGHETDTLLFHLDML
                     GASHLDQTSMLYLQPLQTDVFKCIPRIQPQDPGYAEIRQCLLTIFELVRHQPPYFELP
                     LKSRLFDFFYLLYRYRYIVRKNTDDMYRKNEKLRLLIEYIQQHYAENITIEQLAEQMG
                     YTKTHFMTIFKQQTGTSCMEFIIQVRLRAATELLTNTIKPVLEIATDVGFNNLSNFNR
                     QFKQYYHTTPSSYRKQLRKNRTCTNFDK"
BASE COUNT         3026 a         2055 c         2497 g         2975 t
ORIGIN      
        1 aggagtaaaa aatggctgaa acaaaagtaa tggccttgcg tgaagcgatt aacttggctc
       61 aaagcgaaga aatgcgtaag gatgaaaaag tattcttgat gggtgaagac gtcggtatct
      121 acggcggtga ctttggtaca tctgttggta tgttggacga gtttggtcca aaacgcgttc
      181 gtgacacgcc tatctctgag gctgcaatcg ctggttctgc ggttggtgcg gctcaaacag
      241 gtcttcgtcc aatcgttgac ttgactttca tggacttcat cacaattgcc cttgatgcaa
      301 ttgttaacca ggctgccaaa accaactaca tgtttggtgg tggcttgaag acacctgtaa
      361 ccttccgtgt ggcatctggt tcaggtatcg gttcagcggc acagcactca caatctcttg
      421 aagcttggtt gactcacatt ccaggtatca aggtggttgc accaggtaca gctaacgatg
      481 caaagggtct tttgaaatca tctatccttg ataacaaccc tgttatcttc ttggagccaa
      541 aagctcttta tggtaaaaaa gaagaagtta acttggatcc agatttctac attccacttg
      601 gtaagggtga aatcaaacgt gaaggtacag atgtaaccat catttcatac ggtcgtatgt
      661 tggaacgtgc cttgaaagct gctgaagaag tggctgcaga aggaatcagc gtagaagtgg
      721 ttgacccacg tacccttatc ccattggata aagaattgat catcgaatct gtgaagaaaa
      781 ctggtaaggt tatcttggtt aacgatgctt acaaaacagg tggtttcatc ggtgaaatcg
      841 catcaatcat cacagaaagc gaagcctttg actacttgga tgcaccaatc atccgtatcg
      901 cttcagacga tgtaccagtt ccttacgcaa acattcttga aaacgcagta ttgccaaacg
      961 tagagaaaat caaagcggca atctataagc aagtaaacaa gggttaatct tatcgtcatc
     1021 cgcttatctt ttctggctgt gttgatttgg attgcctaac gtcagttatg cctgcactaa
     1081 atcgctttat cagaaaagca atcggaaaga cgataaccct aatatgctag aaataaaatg
     1141 ttctagcatg actaagaatg cttggaaaag aattgagccg cttcgattat cgaagtctgg
     1201 tttggttctt gacaaggtaa gaaactgaaa gaaaggaatt gaacccgtcc ggaaaactcg
     1261 gaaaaaagat aaatcgttac gaaaatcagg attttcgacg atttcctaga ttccctcgtt
     1321 ttctgttcgg acttggtatc ttaataatgg ccattgaaat cattatgcct aagctcggtg
     1381 tggacatgca agaaggtgaa atcatcgagt ggaaaaaaca agagggcgat ttcgtcaacg
     1441 aaggcgacgt catcttggaa atgatgtctg acaagaccag catggagctg gaagcagaag
     1501 agtcaggtgt cctattgaaa attgttcacg gaaacggtgc taccgttcct gtaacagaag
     1561 ttattgccta catcggtgca gaaggtgaaa ccgtcgaagc aggcgcttca tctgctcctg
     1621 ctgttgaacc agcagcggct attgaagaag tacctgcggg tcgtacacca gtaatcgttg
     1681 ctcctgcaac tgctgccaaa ccacaaggta gtggcaaggt gcgtgcaacg ccagctgcgc
     1741 gtaaattggc gcgtgagctt ggaatcgact tgggtcttgt tccaggaaca ggtgctaacg
     1801 gtcgtgtcca caaggttgac gtcgaagact tcaagggagc agctccaaaa gcaactccgc
     1861 ttgcagcccg tattgcagca gaccaaggtg ttgacttgtc aacccttaca ggttcaggtg
     1921 tcaacggtaa gattgtcaag aacgacgttc ttgcagtact tgctcctgca gctgtagaga
     1981 ctgctgctcc agcaccgaaa gcagaagaaa aaccagctaa agaattgcca gaaggcgttg
     2041 aaatcatcaa gatgagccca atgcgtaagg cgatttcaaa aggtatggtc aactcttact
     2101 tgactgctcc gacatttaca cttaactacg atattgacat gaccaacctc atggcacttc
     2161 gtaagcaagt ccttgagcca atcatgaaca agactggttt gaaagtaacc tttacagact
     2221 tgatcggtct tgcggttgtt cgtactttga tgaaagaaga acaccgctac atgaacgcat
     2281 ctttgattaa cgatgctcaa gaaatcgaat tacacaagtt cgtcaacctt ggtatcgcag
     2341 tgggtctaga tgatggcttg gtggttccag ttgttcacgg cgcagataag atgagcttgt
     2401 ctgactttgt ggtggcttcg aaagatgtca tcaagaaggc tcaatctggt aagttgaagg
     2461 gggctgaaat gtcaggttcg accttctcta tcaccaactt gggtatgttt ggtactaaaa
     2521 cctttaaccc aatcatcaac caaccaaact cagcaatcct tggtgtagca tcaacagttc
     2581 aaacaccagt tgttattgac ggcgaaatca aaatccgtcc aatcatggca ctctgcttga
     2641 ccatcgacca ccgtatcatt gatggtatga atggtgctaa gttcatggtt gacttgaaga
     2701 acttgctgga aaacccattg gaattgttga tctgagataa gatgcaaaag gcgttaagaa
     2761 aaccgtatga aaataggaaa tcaacgatgt gccttgaggc acgaggcgat ttatcttttt
     2821 caataggttt ttagcctgtg ctcaatttgg taaagagaaa aaataaagaa agggaataag
     2881 agcgagagag ttttagcaat tgaacccgag cggaaagctc ggaaaaaaga taaatcgttt
     2941 agagaatcag gattttctac gatttcctat ttttcagtcg ctttctcttc gctcttggta
     3001 tcttaactat ggcaattgaa attattatgc ctaaacttgg tgtagatatg caagaaggcg
     3061 aaatcatcga gtggaaaaaa caagagggtg attttgtcaa cgaaggtgat gttatcttgg
     3121 agatgatgtc agacaagaca agtatggaat tggaagcaga agaatcaggt gtactcttga
     3181 aaatcgttca tggtaacggt acaactgttc ctgtaacaga agtcattgct taccttggtg
     3241 cagaaggcga atcagttgaa gttggtagcg cacctgctcc agctgaggtt gcacaagcaa
     3301 ctgccgactt gaaagcagct ggtttggaag tgcctgcagc tcctgcagca gctccacaag
     3361 ctcctaaggc tgaattggca gctgacgagt acgacatggt tgttgtcggt ggtggtcctg
     3421 ctggttacta cgcagctatt cgtggtgctc aattgggcgg taaaatcgct atcgttgaga
     3481 aatcagaatt tggtggtacc tgcttgaaca agggttgtat cccaactaag acctacctta
     3541 aaaacgctga aattcttgac ggcttgaaga ttgctgctga gcgtggtatc aaccttgctt
     3601 caacaaacta caccgttgat atggacaaga cagttgactt caagaacaag gttgtgaaga
     3661 cattgactgg tggcgttcaa ggtctcttga aagctaacaa ggtaacgatc ttcaacggtc
     3721 ttggtcaagt aaaccctgac aagactgttg tcatcggcga caaggtcatc aaaggtcgca
     3781 gcatcattct tgcaactggc tctaaggttt ctcgtatcaa catcccaggt attgattcta
     3841 aattggtgct aacttctgat gatatccttg acttgcgtga aattcctaag tcactcactg
     3901 ttatgggtgg tggcgtagtc ggtgtggaac ttggtttggt ttacgcgtct tacggtactg
     3961 aagtaacagt tgttgaaatg gctgaccgta tcattccagg tatggaccga gaagtgtctg
     4021 ttgaattgca aaaagtcctt tctaagaaag gtatgaaatt cttgacatca gttggagtat
     4081 ctgaaattgt tgaagccaac aatcaattga caattaaatt gaacgatggt tcagaaatta
     4141 tctctgaaaa agctcttctt tcaattggac gtgtacctca attagctggt cttgaaaatc
     4201 ttcaccttga gttggatcgc ggtcgtatca aggttaatgc ttaccaagaa acttctatcc
     4261 caggaattta tgcacctggt gatgtcaacg gtactaagat gttggcacac gctgcttacc
     4321 gtatgggtga agtggcagct gaaaatgcta ttcacggtaa ccaccacaag gctaaattgg
     4381 acttcacacc agcagcggtt tacacacacc cagaaatcgc tatggttggt ttgactgaag
     4441 atcaggctat cgagaaatat ggtaaagaaa acatccttat cggtcgcaac agctttactg
     4501 gtaacggtcg tgctattgct tctaacgaag cacatggttt cgtaaaagta attgcggaca
     4561 agaaatacca tgaaatcctt ggtgttcata tcatcggtcc agttgcagct gaaatgatca
     4621 atgaagcagc aactatcatg gaatctgagt tgacagttga cgacgtggca gcatccatcc
     4681 acggtcaccc aaccttctca gaggttatgt atgaagcctt cctggatgtg cttggtgttg
     4741 caatccacaa cccaccaaaa cgtaaataat agcattcagt ttatctttta ttacttcgtt
     4801 aactcgattt gccgtactct agtacagcct gcatctcgtt gcctagtact aaaagcaaac
     4861 tgaaatgcta acatctaaaa gaaagtccat atggctttct tttttgtatg ttcatagtat
     4921 agtattagtc tatcgcagtt taggactgtt atttgataga aaaacagact atacttgtag
     4981 tagaaaaagg caaggagaaa acagatgcgc aagattattt ttttagatgt ggacgggacc
     5041 ttggttgact atcataatcg gattccagaa tctgccattc gtgctattcg acaggccaga
     5101 gaaaatggac acctggtcta tgtctgtaca ggtcgaagcc gagcggaaat gcaaccagaa
     5161 ctatgggaga ttggcctgga cggaatgatt ggtggcaacg gctcttatgt cgagcatcaa
     5221 ggtcaggtca tcatgcacca gctcctatca gaagaggata gcagagcgat tgtggactgg
     5281 ctccatgaac gcggcttgga attttatctg gaaagcaaca acgggctctt tgccagtgaa
     5341 aatttccgtg aacgtgcccg tgaaaccctg cgaatttatt ccatgaacaa ggggaaaact
     5401 gcagaagagg tggccactca ggaagtagat gacgttatac acggcatgat ttttgatagt
     5461 caactctacc gcaatgatct caacaaagtc agttttgtac tagactccta tcaggatcat
     5521 ttggactcta aagaggcctt tccgcaactg gtcgcaaaca cctggggagg tcgcggagag
     5581 tctgccctct ttggcgattt gggtgtaaaa gatattgaca aggcccatgc tatttctgtt
     5641 ttgctggatc acttaggagc tagtcaggca gacactatcg cctttggtga cgctaaaatt
     5701 gatatttcca tgctggacta ttgtgcggtt ggggtcgcta tgggcaatgg cggagcggaa
     5761 atcttggcta tggcagatat gataacggac gatgtagaag aagacggcct ctacaatgcc
     5821 tttgaaagat tgggactact tgacaagtag ctctcagaat cccttgcaaa accgcttaca
     5881 atctgataag ataagtgtag gattgttact gataagcagg caaaacctaa gtagatagtg
     5941 aagggtctgg caacagtcgt atttgctatg tgcttgggtt cttttttatc agtcgaaaaa
     6001 agaggcggta aagtagatgc agattgaaaa agtttataac aacaatgtca ttcaagcaat
     6061 agatcagcag ggccgtgagt tgattgtcat ggggaaagga ttaggttttc agaaaaaagc
     6121 aggggaagaa ttagacacca gcaaaatcga gaaaaccttt gtgctacaaa atgactacca
     6181 acaatcagat ttatcgagtc tgtacctgca aatggagagc ggtgaagtcg aagtagtcaa
     6241 tgccattatc aatagggcag aagaaacttt agaagttcag tttgatttgt ctttgtatct
     6301 agccttagca gaccatttac attttgtatt tcagcgatgc cgtgaaggta tctttattga
     6361 gaacccgctg tcttgggaag tacgtaagtt ttatccgaag gaataccaaa ttggtttaga
     6421 agccttgcag ttagtgcagg agcgtttagg cttggaacta ggaaagggag aggcatcgtc
     6481 tatcgctctc catctggtaa atgctcagaa gaatggtgct tttggcaagg aaacccaaac
     6541 cattagtaaa atcgtgactc agattttaga cattgtccgc cttcattttg gtagtgtgac
     6601 ctacgaagaa gataccagct atcatcgatt tgtaacacat attcagtatt ttgctcaacg
     6661 tgtggccaat ggtgtggtcg aaggggtcaa tgatgcattt ttgtatgaac aggtgatggc
     6721 taactatcct gattcatttg cttgtacgac taagattcgt cagcatattg cggaagttta
     6781 ccattttgaa atgtccaaag atgaacaagt ctatttgacc atccatatcc agcgcttaaa
     6841 aacgagttag aaaaaaatca aaaaaacttt agaaaacgct tgcaaaaaaa tttgtaatgt
     6901 gttattatta tttcaacaaa aggattgtta ctggttagca ggcaaaacct aaatatatac
     6961 aagatactct ttttgacgta gttcaagggg cagtgtttgg tatatgttta ggtttttctg
     7021 tttttcccag aaaagaaagg agccgaaaaa tggctaagga ttatacagat ttagctacag
     7081 atattgtagc tcatgttggt ggaaaagaaa acatcagcag tttgaaacac tgcgtgactc
     7141 gtcttcgttt tggtctcaag gatgagagca aggctgatac agactacttg aaggcgcgtg
     7201 atggcgttgt gacagtagtt caagcaggtg gtcaatacca agtggttatc ggcaaccatg
     7261 tacctgatgt ttatgcagcg gtacaaaaag ttgctggtat ctcaggggat ggcgccttag
     7321 atattgatga aggtgacgga ccgaagggaa atctgtttga ccgatttgtt gacctagttt
     7381 caggtgtttt ccagccttct ttaggagtgc ttgcagctgc agggattatc aaaggattgg
     7441 tatccatcct agccgctaca atgggtttga acaatgataa tagcgcccta tatgctattt
     7501 taaacacagc tggagatgga ttcttccaat tcttgccaat cgtcttagct ttcaatgctg
     7561 cacgaaaatt cagaatgaat gaatttactg gtatggcaat tgctatggca ttggtttacc
     7621 caactctgcc agggacattt gcgactctaa aagaagcggg tctaaataat gtttttggca
     7681 taccatttgt acttccagca gcaggtagct acttgcaaac tgtgatgcct gtcctacttg
     7741 ctgtctggat tgcttctcat attgagaaat tcatgaagaa aattacgcct gacgtagtta
     7801 aggtcttcat tgtaccgttt gtgacaattc taatttcagt tccattgaca ttcttggttg
     7861 taggtcctgt cgcgaactac gcttcagatc ttttgtctgc aggctttact gcggttatgg
     7921 gctttagtcc actactttac ggtattctac ttggtgcttt atggcaagta atggttatgt
     7981 tcggacttca ctgggccttg gtaccacttg caatacttca gttttctcaa aacggatggt
     8041 caaatatttt gttagctgca gcgcttccaa actttactca aactggtgtt cttttggcaa
     8101 ttatgatgaa aaccaaagaa tcaaaagtga agtcagttgc gatgcctgca ttcatttctt
     8161 caatctttgg tgtcactgag cctgctatct atggtgttac tcttcctatg aaaacaccat
     8221 tctacatttc ttgtgcagtg tcagcagtaa ttggtgcagg tctctcattc tttgatttga
     8281 aaaactatgc tatgggtgct ctcggtatct ttatgtatcc aggttatgtt agtcctgaac
     8341 taggtgtgac tccaatgtgg attatgatgg gcttctcttt ggcggcaatt gttttatcat
     8401 tcgctatcca aatggctctt ccagttcctt acttatatgg agaaccaact gaaaaaaagt
     8461 ccgttgaaga aacaaaggag tctgttccag aattaaaaga aattaaacaa gagatcattg
     8521 ctagtccact aatcggtaaa gtagttaaat tagaagatgt tcctgatgca gtctttgctt
     8581 caggagctat ggggaaagga attgctatcg atcccctaga tggaatcctt gtatcaccag
     8641 cgaaggctga agtcactctt ctattcccta ccaagcacgc cattggtctt cggacggaaa
     8701 atggggcaga gctactcatc catatcggta tggacacagt ctctctagct ggtaaaggct
     8761 ttgaatcctt cgtccaagta ggtgaccaag ttgaagcagg acaaaaattg ctggaatttg
     8821 acctccacca aatcaaagca gctgacctac cggtcatcac tccaattatc gtgaccaata
     8881 cagctgacta tgaggacatc ttggtaaccc aagaaagcca aatcaacagt ggtgattacc
     8941 tgttgacaac ggtcaaataa aaaataaagt ggttggcagt gccagtcact tttttgaatc
     9001 ttgtttagcg ttgaagaaaa tccttatcct gctctggaat gcccgcctca tcacttttat
     9061 agcgttcagc ggtgacagtt agctggtact tactacgtaa gtctaggatc tcctgcatca
     9121 ttggagaagc atggtggatg tccaaagctg cttggtctgt ccagctatca accaagagga
     9181 ttgaatgagg atcatcgaga ggtgcaaagt attgataacg tgtatttcca gcctgctggc
     9241 gaatgcggtc ggcaatgcca ctagcttcca tttcctgagc gaaagcacgt gcattgtcag
     9301 ctggtccggt gtagtagagg tgcatggtaa tcatagtata agatatagag ggcgctaagc
     9361 ggccacagac aaaatatagt cttttagttt acttttagta ctaggcaatg agctgcaggc
     9421 agtactgaag tacggcaaag cgaatgaacg aagtattaaa agaaaaacta aatgacgtag
     9481 gaaacagaac gacgatgcaa gcgtccagtt ctcgtttatc tttttggttt agtccgtagc
     9541 ccgtattcag ttcaactaaa tacagaggcc ctagtccttt ctattattta tcaaagttag
     9601 tacaggtcct attcttccgc aattgtttgc gatagctgct aggggtggta tggtagtatt
     9661 gtttgaactg gcggttgaag ttggacaggt tattaaagcc aacatcggtc gcaatttcaa
     9721 gcacaggctt gatagtgttt gtcaggagct cggtagctgc tcgcagtcgg acttggatga
     9781 taaattccat acaggaagtt cctgtttgtt gcttgaagat tgtcataaaa tgggtttttg
     9841 tatagcccat ttgctctgct agctgttcga tggtgatatt ttctgcataa tgttgttgga
     9901 tgtattcaat caacaaccgc agtttttcat ttttccgata catatcatcg gtattttttc
     9961 ggacgatgta gcggtagcgg tagagcaggt agaagaagtc aaagagtcgg ctcttgagag
    10021 gtagctcaaa gtaaggcggc tggtgtctga ccaactcaaa aatggtgagc aggcattgtc
    10081 taatctctgc gtagccagga tcttggggct ggatgcgtgg aatacacttg aaaacatctg
    10141 tctgtaaggg ttgtaggtag agcatactgg tctgatctag gtgagacgcc cccagcatat
    10201 ccaagtgaaa gagaagggta tcagtttcgt gcccccgttg ctcaatcgga tgaatagaat
    10261 gcgcagcatt gggtcgaatc aggataatat caccagcttg actattgaaa tagtcatagt
    10321 cgatatggta acgggctgtc cctccgtaaa catagttgat ttcaaattca ttgtgccaat
    10381 gaaagagaat gtcaggaatg ccatccgtga cataggtatt gtaatactta tagggtaaca
    10441 aacgactatc gtagtcaatg gtatgtttaa actgtttatc cgttaatggc ggttgcatac
    10501 tttcctcctt ttcgtaggat agtatcatta tagcatatta tttgagaaga aaa
//
LOCUS       DBNFEG010000068        10415 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.068, whole genome shotgun sequence.
ACCESSION   DBNFEG010000068 DBNFEG010000000
VERSION     DBNFEG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10415
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.068"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(207..1697)
                     /gene="lysS"
                     /locus_tag="AC1PNG_001148"
     CDS             complement(207..1697)
                     /gene="lysS"
                     /locus_tag="AC1PNG_001148"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIP6701656.1"
                     /translation="MSTEHFEELNDQQIVRREKMTALAEQGIDPFGKRFERSANSAEL
                     KAQYEDKSKEDLEELGQTAIIAGRIMTKRGKGKAGFAHIQDREGQIQIYVRKDDVGEE
                     NYEIFKKADLGDFIGVEGDVFKTNVGELSIHARKLTHLSKALRPLPEKFHGLTDIETR
                     YRKRYLDLITNRESFDRFVTRSKIISEIRRYLDGLGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADYLDIMDLTEGIIQHTAKAVVGDGPVTYQGTEIAIHEPFKRIHMVDAIKEQTGVD
                     FWQEMSFEEAKALAAEHKVPVEKHHTEVGQIINSFFEEYVEATLIQPTFVYGHPVAVS
                     PLAKKNDEDPRFTDRFELFIMTKEYGNAFTELNDPIDQLERFEAQAKAKELGDDEATG
                     VDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(1800..2426)
                     /locus_tag="AC1PNG_001149"
     CDS             complement(1800..2426)
                     /locus_tag="AC1PNG_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIP6701657.1"
                     /translation="MKIYFVRHGKTEWNLEGRFQGYSGDSALLPESYQDLEKLGKYLA
                     EIPFDAIYSSDLQRAHSTAVEIAKANHHCQTVLTTQQLREWNFGTLEGSKMAIFRAIY
                     PKQAWALKHNLALFNNDLFEAESVRQVTQRMVDFVQSLKGQDMETVLIVSHGAFLTAS
                     IHRLLGFPPAQLRHRGGLDNASVTILETSDFENFTELAWNDTSYKAKQ"
     gene            complement(2429..2911)
                     /locus_tag="AC1PNG_001150"
     CDS             complement(2429..2911)
                     /locus_tag="AC1PNG_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIP6701658.1"
                     /translation="MAKKVKIKKTLVDQILDKAKINHDSLVLNAFEGQLPPGIEEHEI
                     YKTLALTGDKTGPIIGIVPITEHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNFPIYIDQSALELGHLIVSAGEIGRSIRIDSQVLADFVKADFADLIEGRD
                     "
     gene            complement(2911..3753)
                     /locus_tag="AC1PNG_001151"
     CDS             complement(2911..3753)
                     /locus_tag="AC1PNG_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HIP6701659.1"
                     /translation="MASWISRIIKGMIIALGFILPGVSGGVLAAILGIYERLIGFLAN
                     IRKDFKENFLYFVPVGIGGILGIALFSFPVEFLLQHFQVPVLWAFAGAIVGTIPSLLK
                     ESTQKSKRDSIDLAWLIGTFIISGLLLYNLSDLVGTLPATFASFVLAGALIALGVLVP
                     GLSPSNLLLILGIYTPMLNGFKSLDLFGTFLPIAIGGVLAMVAFSKAMDHALKVYHSR
                     VYHFIIGIVSSSTLLILIPQASNKESISYAGANFVTWIAAIVLFILGAWLGLWMSKLE
                     EKYK"
     gene            complement(3856..4404)
                     /gene="thiT"
                     /locus_tag="AC1PNG_001152"
     CDS             complement(3856..4404)
                     /gene="thiT"
                     /locus_tag="AC1PNG_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HIP6701660.1"
                     /translation="MSTSKLPILAEVAIFSAIALVFDKIPLFTMPQGGSVSLVMLPIL
                     LLALRHGLGVGVLTGGIVGTIQLFYGGYFLNVFQVFLDYALSYAGIGLAGLVAPTLSK
                     QKDLKNATLIITLASFLGGSIRLLATFLSGIIFYADYAPDGMPVWFYSFTYNISYILP
                     STAIASILLILLYRARPGFYNL"
     regulatory      complement(4463..4558)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(4582..5436)
                     /locus_tag="AC1PNG_001153"
     CDS             complement(4582..5436)
                     /locus_tag="AC1PNG_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HIP6701661.1"
                     /translation="MRKKLNPMIVVGFFLSFFALIFITGVTGNTVNKTEVANEPAVTQ
                     SNTSTSSKTTNSSSSYYIANALEMKPIVDVSAWQRPSEIDYDTLSKNISGAIVRIQSG
                     SHTKNENTATDKNGLDKSFDTHIKEFQARNIPVAVYAYVTGNSIENMREEARSFYKAA
                     NKYKPTFYWLDVEEYTMDDMNAGVEAFRKELETLGAKNIGIYVGTYFMEDHSINVDKF
                     DAIWIPTYGDDSGYYNAAPNTDLNYDLHQYTSRGYVNGFEHHLDLNIITTLKDPNEVY
                     QKLFTTPE"
     gene            5549..6052
                     /locus_tag="AC1PNG_001154"
     CDS             5549..6052
                     /locus_tag="AC1PNG_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701662.1"
                     /translation="MHDKSEKVRRVSQLVVLSSLTIVLRVVFGPFPNIKPLMAIFLIV
                     LSYFSLLESWILMILVMVGSGLLFGFGTVVLWQVLSFAFIQFIWWLAIGPFIKKENIP
                     LWLQSLLAGILVFLYGLTISFLSATQFRTSPIVFWLNGLVFDSLHAASTVLFYPIIDY
                     IFRRFYK"
     gene            6049..6414
                     /locus_tag="AC1PNG_001155"
     CDS             6049..6414
                     /locus_tag="AC1PNG_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HIP6701663.1"
                     /translation="MKKISIMLMVVFSLFLAACTNTTEKQSESAIEVTLQITTKDKAN
                     HQEVTAEEGDSVMDVLKEHHDIVEDNGLITEIDGVSQDASTNTYWMYKVNGQMADKGA
                     KDLTVANGDEIEFYLETFE"
     gene            6551..7765
                     /gene="ftsW"
                     /locus_tag="AC1PNG_001156"
     CDS             6551..7765
                     /gene="ftsW"
                     /locus_tag="AC1PNG_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division peptidoglycan polymerase FtsW"
                     /protein_id="HIP6701664.1"
                     /translation="MKIDKRQLLNYSILIPYLILSVIGLVMVYSTTSATQIINGGNPF
                     RTVINQAGFWIVSLVAIYTIYRMKLSFLRKKAVIYSVILVEVFLLAISRLFPPINGAH
                     GWIPLPIAGTLQPAEYLKLIIIWYLAHEFAKQQADIRTYDYVSLIKGSWIPKEFTDWR
                     VVSLLLLGLVATLPDLGNATIIVLIMVVMISVSGIAYRWFSTAVLGIVAASTVILGTI
                     RILGVETVEKIPLFGYIARRFAAYFDPFGNATNSGLQLTHSYYAMSNGGWLGRGLGNS
                     IEKKGHLPEAHTDFAFSIVIEELGFVGASLILALLFFLIIRIIIVGVRARSPFNAMMA
                     LGMAGMLLIQTFVNIGGISGIIPATGVTFPFLSQGGSSLLIISIGIGFVLNIDASEKR
                     RLIEEEIERTLI"
     gene            7786..>10415
                     /gene="ppc"
                     /locus_tag="AC1PNG_001157"
     CDS             7786..>10415
                     /gene="ppc"
                     /locus_tag="AC1PNG_001157"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HIP6701665.1"
                     /translation="MAIQKLENYNNKEAIREEVTILTSILEKVAAQMMPAETFAKIVE
                     LSQLSRQDDYQALIAIISQLNNDELAVISRYFAVLPLLINISEDVDLAYEINHQNNID
                     QDYLGKISTTIDMVSKQENAAEILEKLNVVPVLTAHPTQVQRQSMLDLTKHIHELLRR
                     YRDVKLGLLNKNKWEDELRCYIEIIMQTDMIREKKLKVTNEITNVMEYYNSSFIKAVT
                     KLQREYKRLAAEKGIVLNNPRPITMGMWIGGDRDGNPFVTAETLKLSALTQCEVIMNY
                     YDDQLYKLYRNFSLSTSIVNVSTAVKMLADLSEDSSVYRENEPYRRAFHYIQMKLANT
                     RDYLVHNKPSDVRYSNVAEFKADLLAIKQSLVENKSMALLKGDFTELLEAVEAFGFYL
                     ASIDMRQDSSIHEASVAELLASARIVEDYSSLSEEAKCHVLLKQLETDPRILSATHMP
                     KSEQLEKELAIFAAARELKDKLGEEIIKQHIISHSESVSDLLELAVLLKEVGLVDVDK
                     ARVQIVPLFETIEDLDNAPDTMRQYLQLDLAKRWIAGNKYYQEIMLGYSDSNKDGGYL
                     SSGWTLYKAQNELTQIGQDYGVNITFFHGRGGTVGRGGGPSYDAITSQPFGSIKDRLR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSATLDRMVTQMITDPNEIDGYRETMDEIVSDS
                     YRIYRDLVFNNPHFYDYFFAASPIREVSSLNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QNRVMLPGWYGVGSSFKRFIDKHPDNLSKLQKMYESWPFFRSLLSNVDMVLSKSNMNI
                     AFEYAKMCESEEVRNIFHVILDEWQLTKEIILSIEQNDELLAELPFLKASLDYRMPYF
                     NVLNYIQIELIHRLRRGELS"
BASE COUNT         3008 a         1958 c         2279 g         3170 t
ORIGIN      
        1 cctgactatg tggttgggat ttttttgcat gcaaaaacac tcttggaatt tccaagagtg
       61 ttttaagtaa cggtgttaaa cagtttgtaa tttttatttc atcgtcggga gtgaaaaggt
      121 ctagtagacc ttttcagcca atcgctaaga aactggagag cgattggtac attctagtga
      181 aaacaatcct agcaagctac tcttacttac ttcatagtcg gaaataggag tacatcccga
      241 atagttgttg tgtctgttaa gagcatgcag aggcggtcga taccgattcc aagtccacct
      301 gttggtggca taccgtattc gagggcttcg atgtagtcgt agtcaacgcc tgtcgcttcg
      361 tcgtcaccga gttctttagc tttggcttgg gcttcgaagc gttccaattg gtcgattggg
      421 tcgttcaatt ctgtaaaggc atttccgtat tccttggtca tgatgaagag ctcgaagcga
      481 tctgtgaagc gtgggtcttc atcgtttttc ttagccagtg gggatacggc tactgggtga
      541 ccatagacga aggttggttg gataagagtt gcttcaacgt attcttcaaa gaagctgttg
      601 atgatttgac caacttctgt atggtgtttt tccacaggga ccttgtgctc agctgcaagg
      661 gcttttgctt cctcgaagct catctcttgc cagaagtcca cgccagtttg ttccttaatg
      721 gcatcaacca tgtggatacg cttgaatggc tcgtggatag caatttcagt gccttgataa
      781 gtaacaggac catcgccgac aacagccttg gcagtgtgct gtataatacc ttcggtcaag
      841 tccatgatgt ccaagtaatc tgcataagct tggtagacct cgattgacgt gaactcaggg
      901 ttgtgagtcg catccatacc ctcgttacgg aagatacgtc cgatttcata aacgcgttcc
      961 ataccaccga caatcaagcg tttgaggtgg agttctgtcg cgatacgaag gaccatgtca
     1021 atgttttggg cattgtggtg ggtgatgaat ggacgagctg cagcaccacc agcttcattg
     1081 tgaaggacag gtgtttccac ttccaagaaa ccaagtccgt caaggtaacg gcggatttct
     1141 gagataattt ttgagcgggt cacaaagcga tcaaagctct cgcggttagt aatcaagtcc
     1201 aaataacgtt tgcggtagcg ggtctcgatg tctgtcaaac cgtggaattt ttctggcaat
     1261 gggcggagcg ctttagacaa gtgagtcaac ttgcgagcat ggatagacaa ctcaccaacg
     1321 tttgtcttga agacatctcc ctcgacaccg ataaagtcac caaggtctgc ttttttgaag
     1381 atttcatagt tttcttcgcc aacatcatcc ttacgaacgt agatttgaat ctggccttcg
     1441 cggtcttgaa tgtgagcaaa acctgcctta cccttaccac gcttggtcat aatacgacct
     1501 gcaatgattg ctgtttgccc taattcttcc aagtcttctt ttgatttgtc ttcgtactgg
     1561 gccttcaatt cagctgagtt ggcagaacgc tcaaaacgtt ttccaaatgg gtcaatgcct
     1621 tgttcagcaa gagccgtcat tttctcacga cggacaatct gttggtcatt taattcttca
     1681 aaatgttcag ttgacataaa aattttattc ctccaaagtc tgttatttct attttatcat
     1741 agaagagaga aaaaatcagt ataaattgct aataggatag gaaaaaacag cagaaaattc
     1801 tactgttttg ctttatagct ggtatcattc caagctaatt ctgtaaagtt ttcgaaatcg
     1861 cttgtttcta aaatagtaac cgatgcatta tccaaaccac ctcggtgacg cagttgggct
     1921 ggtggaaaac caagcaggcg atggatagaa gcggttagaa aggcaccatg gctgacgatg
     1981 aggactgttt ccatgtcttg ccctttcagc gattggacaa agtccaccat tcgttgagtt
     2041 acctggcgaa cgctctcagc ttcaaataag tcattgttaa aaagtgctag attatgtttg
     2101 agtgcccagg cttgtttggg gtagatggca cgaaaaattg ccatcttgct tccttctagc
     2161 gttccaaaat tccattcacg tagttgttgc gtggttagaa cggtttggca atggtgattg
     2221 gctttggcaa tttctacggc agtgctatgt gcgcgctgca aatcactaga ataaatagca
     2281 tcaaagggga tttcagcaag gtatttgcct agtttttcta aatcctgata agattcaggc
     2341 aagagagctg aatctccaga atagccttga aagcgtccct ccaaattcca ttcggtctta
     2401 ccgtgacgga caaagtagat tttcaagtct agtccctccc ctcgatcaaa tcggcaaagt
     2461 cagcttttac aaaatcagct aatacctgac tgtcaattcg aatcgaccga ccaatttctc
     2521 cagcagagac aatcaagtga ccgagttcaa gggcagattg atcaatgtag attgggaaat
     2581 tatgcttctg acgaatgcct acgggattgt tggcaccatg gatgtagcca gttgtctttt
     2641 ccaagtcctt ttgcggaatc atactcactt ttttattgcc agagatcttg gctaattttt
     2701 tctcagacag gtgttctgtg attgggacaa tgccgataat tggtccagtt ttatctccag
     2761 ttaatgccag tgttttgtaa atttcgtgtt cttcaattcc tggtggaagt tgaccttcga
     2821 aggcatttaa aacaaggcta tcatgattga tcttagcctt gtccaatatt tggtccacca
     2881 aggttttctt tattttaacc ttttttgcca ttatttatat ttttcctcaa gtttactcat
     2941 ccaaagccct aaccatgctc ctagtataaa taggacaata gcagcaatcc atgtaacaaa
     3001 gttcgctcca gcataggaaa ttgactcttt attacttgct tgtggaatca aaattaggag
     3061 agtagaagat gaaacgatac caataataaa gtgataaaca cgtgaatggt atactttcag
     3121 agcgtggtcc attgcttttg aaaatgcaac cattgcaaga acaccaccga ttgcgattgg
     3181 taggaaggta ccgaatagat caagtgattt gaaaccattt agcattggcg tgtagatacc
     3241 caaaatcaat agtaagtttg aagggctgag tccaggaaca agtaccccta aagcgatcaa
     3301 ggcacctgcc aatacaaaac tagcaaaagt agctggaagt gtcccgacaa gatcgctcaa
     3361 attgtaaagt aggaggccag aaataatgaa tgtcccaatc aaccaagcca aatcaatgct
     3421 atctcgtttg ctcttttgag ttgattcttt aagtaggcta ggaatagttc ccacgattgc
     3481 tcccgcaaat gcccacaaaa ctgggacttg gaaatgttgc aataaaaatt ctacagggaa
     3541 agagaaaagt gcgattccca aaattccacc aattcctaca ggaacaaaat aaagaaagtt
     3601 ttccttgaaa tcttttcgaa tatttgctaa aaatccaatt agtcgctcat agattccgag
     3661 gatagcagcg agtacccccc cagagacacc aggtaagata aagccgagtg cgataatcat
     3721 acctttaata attcttgaaa tccaagaagc cataatttcc tccaaaatgt attataacgt
     3781 tcctattata gcataaagca agtaaaaata attaaaaaga ctgcctgtta gataaaatcc
     3841 agcaggcagt tttggttaaa gattgtaaaa acctggtctt gctctataga gtaaaatgag
     3901 cagaatagag gcaattgcag tggaaggaag aatatagctg atattgtagg taaaggaata
     3961 gaaccagaca ggcataccat ctggagcgta gtctgcgtag aaaataattc ctgataagaa
     4021 ggtggctaac aatcggatgc ttcctcctag aaaactagcc agagtaatga tgagagtagc
     4081 atttttaaga tctttttgtt tggatagggt gggtgccact aaacctgcta aaccaattcc
     4141 ggcgtaagaa agtgcgtagt caagaaaaac ttgaaaaacg tttaggaagt agccaccgta
     4201 gaaaagttga atggttccga caataccacc agtaagaacc cccaccccta gtccgtggcg
     4261 cagagcaagc agtaagattg gaagcatgac taaagacaca gatccacctt ggggcattgt
     4321 aaagagcggg attttgtcaa agacgagggc aattgcagaa aagatagcga cttcggctag
     4381 gatgggtaat tttgatgtgg acataaaaag actcctttgt agatggtcaa caaaggagtg
     4441 tttatagcag agtatagtat cattctataa agcacacaat tcctacgcta gttttaacta
     4501 gatcaggttc gaggatttcg cagttgcgat ctcagccgaa gcactccttt gtgtatatgt
     4561 aattagtgag attgtagcat tttattcggg tgttgtaaat agtttctgat aaacttcatt
     4621 aggatccttc agggtagtaa taatgttgag atctaggtgg tgctcaaagc cgtttacata
     4681 accacgtgag gtatattggt gaagatcgta gttcaagtca gtatttggag cggcattgta
     4741 gtagccagaa tcatcaccgt atgtaggaat ccaaatggcg tcaaatttat ccacattgat
     4801 gctgtggtct tccatgaaat aggttccgac gtaaattcca atgtttttag cgccgagagt
     4861 ttctaactct tttcgaaaag cctccactcc agcattcata tcatccatgg tatattcttc
     4921 aacgtctaac caatagaagg ttggcttgta tttatttgcg gccttataaa agcttcgagc
     4981 ttcctctcgc atgttctcaa tactatttcc cgtgacatag gcatagacag caacaggtat
     5041 atttcttgct tggaattctt taatatgggt atcaaaggat ttatctaacc catttttatc
     5101 agtcgcagta ttttcgtttt tagtgtgtga tccactttga atacgaacga ttgcgccaga
     5161 aatgtttttg ctaagggtat cgtagtcaat ttcagacggt ctctgccaag cagaaacgtc
     5221 gacgataggt ttcatttcga gcgcatttgc aatgtaataa cttgagctag aatttgttgt
     5281 tttagaactt gtactggtgt ttgattgtgt gactgcgggt tcgttagcta cctccgtctt
     5341 atttacagta tttccagtca ccccagtgat gaaaataaga gcaaagaagg ataggaaaaa
     5401 accaacaaca atcatcggat ttagtttctt tctcatctcc tcaattctac cgtaaaatca
     5461 tgaaaaaatc aaaaaaatac gattcaagaa atagtaaaag attatctgaa cttgacataa
     5521 taaagggaat aaggtaggct aaattggtat gcatgacaaa agtgaaaaag taagacgtgt
     5581 tagtcagcta gttgttctat cctctttgac gattgtttta agggtggttt ttgggccatt
     5641 tcccaatata aaacctctca tggctatttt cctcattgtt ctgtcctatt tcagtttgct
     5701 ggagagctgg atattgatga tactagtgat ggtagggagt ggcttgctat ttggatttgg
     5761 cacagttgtt ttgtggcaag ttcttagttt tgcttttatc caatttatat ggtggttggc
     5821 aatcggtcct tttatcaaaa aagagaacat tcccttgtgg ctacagagtc ttctggcggg
     5881 gattcttgtc tttttgtatg ggctaacgat aagtttttta tctgctacac agtttaggac
     5941 ttcccctatc gtcttctggc ttaatggtct agtctttgat agtttacacg ctgcttcaac
     6001 agttttgttt tatccaatta ttgattatat ttttaggaga ttttataaat gaaaaaaatt
     6061 agtattatgt tgatggttgt cttctcgctc ttcttggctg cttgtacaaa cactactgag
     6121 aaacagagtg aatcggctat cgaagtcact ttgcagatta caaccaagga caaagcgaat
     6181 catcaggaag tcactgcgga agaaggtgat agtgtcatgg atgtgcttaa agaacatcat
     6241 gacattgtag aagacaatgg tttgataacg gaaattgatg gtgttagcca agatgcgagt
     6301 accaatacat actggatgta taaagttaat ggccaaatgg ctgataaagg ggcgaaagac
     6361 cttacagttg cgaatgggga tgaaattgaa ttttatcttg aaacatttga atagatatga
     6421 aaggcttcgg cctttttctt gtcgacaaaa gtcagttccg aatgtttgca gagcaactta
     6481 gaaagtgata agtattttta tacaaagtat gctataataa gagctatact cgaagaaggt
     6541 tggataagca atgaagattg acaaacgcca gttgttgaat tattccatac tgattcccta
     6601 tctcatctta tcggtgattg ggttggtcat ggtctattcg acaaccagtg ctacgcaaat
     6661 tattaatggt ggaaatccat ttagaacagt gattaaccag gcaggattct ggattgtcag
     6721 cttggtagca atctatacga tttatcgtat gaaattaagt tttttacgca agaaagccgt
     6781 tatctattcg gttattttgg tagaagtatt tctgttagct atttctcggt tgtttcctcc
     6841 gattaacgga gctcatggct ggattcctct gccaatcgca ggaaccttgc agccggcaga
     6901 atatttgaaa ttaattatca tttggtatct ggcacatgag tttgcaaaac aacaggcgga
     6961 tattcggact tatgactatg tatcgttgat taaaggaagt tggattccga aagagtttac
     7021 agattggcgg gtggtcagtc tgctcctcct tggtctagtt gcgacactgc cagacttggg
     7081 aaatgcgacg attattgtgc tgattatggt cgtgatgatt agtgtgagtg ggattgccta
     7141 tcgctggttt tcaacagcgg ttttgggaat tgtggccgcc tcaacagtta ttctagggac
     7201 gattcgtatt ttgggtgtag aaacggtgga aaaaattcca ctctttggtt atattgctcg
     7261 tcgtttcgcg gcctattttg atccatttgg taatgccacc aactcaggtc ttcaattgac
     7321 tcattcttac tatgcgatga gtaacggtgg atggcttgga cgtggcttgg gaaattcgat
     7381 tgagaaaaag gggcatctac cagaggccca caccgacttt gcgttttcga ttgtcattga
     7441 agaactcggt tttgtcggag ctagtttaat tctagcctta ttgttctttt tgattattcg
     7501 aattattatt gtaggcgtcc gtgctcgtag tccatttaat gcgatgatgg cacttggaat
     7561 ggcaggtatg cttctaattc aaacatttgt caatatcgga ggaatttcag gaattattcc
     7621 tgcaacgggg gtaactttcc ccttcttgtc acagggtgga tcgagtcttt tgattatttc
     7681 catcgggata gggtttgttc tgaatattga tgctagtgaa aaacgtcgat taattgaaga
     7741 agaaatagag cgtacgctaa tttagagata ggaaagaggt aggagatggc tattcaaaaa
     7801 ttagaaaact ataacaacaa ggaagcaatc cgtgaagaag taacgattct aacatctatt
     7861 ttggaaaaag tagcagccca aatgatgcct gcagagacct ttgctaaaat tgtagaatta
     7921 tctcaattgt ctcgtcaaga tgattatcag gcattgattg ctattatcag tcagttgaat
     7981 aatgatgaac tagcggttat ttctcgatac tttgctgttt tgccactttt gattaatata
     8041 tcagaagatg ttgacttggc ctatgaaatc aaccatcaaa acaatattga tcaagattat
     8101 cttggtaaga tttcaacaac aattgatatg gtttccaaac aagaaaatgc agctgaaatc
     8161 cttgaaaagt tgaatgttgt tccagtcttg acagcccatc ctactcaggt tcaacgccag
     8221 tctatgcttg atttaaccaa gcatattcat gagttgcttc gtcgttatcg cgatgtaaaa
     8281 ttgggcttgc tcaataaaaa caaatgggaa gatgagctcc gttgttacat tgaaattatc
     8341 atgcagacgg atatgattcg tgagaagaag ttgaaggtaa ccaacgaaat cacaaacgtt
     8401 atggagtatt ataattcttc ctttattaag gctgtcacta agttgcagcg ggaatacaaa
     8461 cgtttggccg ctgagaaagg aatcgtctta aataatcctc gtccaattac aatgggtatg
     8521 tggattgggg gagaccgtga tggaaatcca tttgttactg cagagacctt gaagctgtcc
     8581 gccttgactc agtgtgaagt cattatgaat tattacgatg atcaactcta taaactctat
     8641 cggaattttt cactctcaac ttccattgta aatgtatcta cagcggttaa gatgctggcg
     8701 gatttatctg aagatagttc tgtctaccgt gaaaatgagc cttatcgtcg tgcttttcac
     8761 tatattcaga tgaaacttgc aaatacaaga gattatctag ttcacaataa gccgtcagat
     8821 gttcgctata gtaatgtagc cgagtttaaa gctgacttat tagccatcaa acaatctttg
     8881 gttgaaaata aatctatggc gctattgaaa ggtgatttta cagaattgct tgaagcggtt
     8941 gaagcgtttg gtttctacct agctagtatc gatatgcgcc aagactcttc tattcatgaa
     9001 gcaagtgttg cggagctatt ggcctctgcc cgtatcgtag aagactactc cagtttatct
     9061 gaggaagcta aatgccatgt tcttttgaaa cagttggaaa ctgatccacg cattctttct
     9121 gcaacgcaca tgccaaagtc agaacaatta gaaaaagaat tggccatctt tgctgctgcg
     9181 cgtgaattga aagataagct aggtgaggaa atcatcaagc aacatattat ttcacactcg
     9241 gagagtgttt ctgatttgct ggaattagct gtcctattaa aagaagttgg tctagtagat
     9301 gtcgataagg cccgtgttca gattgttcca ctttttgaga caatcgagga cttagataat
     9361 gcacctgata ccatgcgtca atatctgcaa ttagatttag cgaaacgttg gattgcaggc
     9421 aataaatact accaagaaat catgcttggc tactcagatt ccaacaagga tggtggctac
     9481 ctatcctccg gctggacact ctacaaagca cagaatgaat tgacgcaaat tggtcaagac
     9541 tacggagtaa acatcacctt cttccacggt cgtggtggta ctgttggtcg tggtgggggt
     9601 ccttcctatg atgcgattac atctcagcca tttggttcca tcaaggaccg gcttcgtttg
     9661 acagaacaag gcgaagtgat aggtaataaa tacggtaaca aagacgcagc ctactataat
     9721 ttagaaatgc tggtatctgc aacgcttgac cgtatggtca cacaaatgat taccgatcca
     9781 aatgaaatcg atggctatcg cgaaaccatg gatgaaattg tatcagatag ctatcgtatt
     9841 tatagagatc ttgtgttcaa taatccacat ttttatgatt atttctttgc cgctagtcca
     9901 attcgtgaag tatcaagtct gaacattggc tcacgtccag cggctcgaaa aaccatcaca
     9961 gaaatcggtg gtttgcgtgc gattccatgg gtattctctt ggtcacaaaa ccgtgtcatg
    10021 ttgccaggtt ggtatggagt tggttcaagt ttcaaacgct ttattgataa gcaccctgat
    10081 aatttgtcta agttacaaaa aatgtacgaa tcttggccat tcttccgttc acttctatca
    10141 aacgtggata tggtattgtc taaatccaat atgaatatcg catttgaata tgctaaaatg
    10201 tgtgaaagcg aagaggtacg aaatatcttc catgtcattt tagatgaatg gcagttgacc
    10261 aaagaaatta ttttgtcaat cgagcaaaat gatgagcttt tagcagaact accgtttcta
    10321 aaagctagtc ttgactatcg catgccttat ttcaatgtgc tcaactatat tcagattgaa
    10381 ttaattcatc gtcttcgtcg tggagaattg agcaa
//
LOCUS       DBNFEG010000069        10120 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.069, whole genome shotgun sequence.
ACCESSION   DBNFEG010000069 DBNFEG010000000
VERSION     DBNFEG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10120
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.069"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            35..1408
                     /locus_tag="AC1PNG_001158"
     CDS             35..1408
                     /locus_tag="AC1PNG_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIP6701666.1"
                     /translation="MFEKILIANRGEIAVRIIRAARELGIATVAVYSEADKEALHTML
                     ADEAVCIGPARSTDSYLNMQAVISAAVVTGAQAIHPGFGFLSENSKFATLCEEVGIKF
                     IGPSGTVMDTMGDKINARAEMIKAQVPVIPGSDGEVLTSQEALAIAEKIGYPVMLKAS
                     AGGGGKGIRKVEKAEDLVPAFESASSEAKAAFGNGAMYMERVVYPARHIEVQILADQH
                     GHVIHLGERDCSLQRNNQKVLEEAPSIAIGQTMRERIGQAAVRAAQSVGYENAGTIEF
                     LLDEAKGEFYFMEMNTRVQVEHPVTEFVTGVDIVKEQIKIAAGQELSVRQEDVRITGH
                     AIECRINAENPAFNFAPSPGKISNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKVI
                     VHGENRFEALMKMQRALYELEIDGVVTNTDFQLDLISDKRVVAGDYDTAYLMEEFLPR
                     YQEDLKK"
     gene            1423..2289
                     /gene="accD"
                     /locus_tag="AC1PNG_001159"
     CDS             1423..2289
                     /gene="accD"
                     /locus_tag="AC1PNG_001159"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIP6701667.1"
                     /translation="MALFRKKDKYIRINPNRSRIESAPQAKPEVPDELFSKCPACKEI
                     LYKKDLGPEKTCQHCSYNFRITAQERLALTVDDGSFEELFTGIETKNPLDFPNYLEKL
                     AATRQKTGLDEAVLTGKATIGGQPVALGIMDSHFIMASMGTVVGEKITRLFELAIEER
                     LPVVLFTASGGARMQEGIMSLMQMAKISAAVKCHSNAGLFYLTVLTDPTTGGVTASFA
                     MEGDIILAEPQTLVGFAGRRVIESTVRENLPDDFQKAEFLQEHGFVDAIVKRQDLPAT
                     IGRLLRMHGGVR"
     gene            2286..3059
                     /locus_tag="AC1PNG_001160"
     CDS             2286..3059
                     /locus_tag="AC1PNG_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HIP6701668.1"
                     /translation="MTSDVARMIRLARDQARLTTLDYATQLFDDFIELHGDRNFRDDG
                     AIVGGIGILAGQPVTVIGIQKGKNLQDNLKRNFGQPHPEGYRKALRFMKQAEKFGRPV
                     VTFINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVAD
                     QVWMLENSMYAVLSPEGFASILWKDGSRAMEAAELMKITSYELEKLQVVDKVILENGR
                     ATKEVLADIKENLVSQLAQLQALPLDKLLENRYQRFRKY"
     gene            3123..3596
                     /locus_tag="AC1PNG_001161"
     CDS             3123..3596
                     /locus_tag="AC1PNG_001161"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6701669.1"
                     /translation="MLVKADLSNAEELLPIQHRAFAALYKTYQDQYNPAIETMDYFQS
                     RFARPNCSYYKIIEEEHTVGLVRTTVAEDAEQGWLGLIGIDPDHRGKGYGHKAMLELE
                     SLYPTVRRWVLCTVLQESKLVSFYEKLGYKAIKTEPEQEGMDMVYMEKWIGDSDA"
     gene            3589..4221
                     /locus_tag="AC1PNG_001162"
     CDS             3589..4221
                     /locus_tag="AC1PNG_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701670.1"
                     /translation="MLEQCRRYLDYQGVHYCKPEKAGALAGDMIALKNVAQAARKEFQ
                     TLSLVLAEHVSPFVPERVSQWMNQAQICRPHFWTYYRFPSASLTDSALAIRLYGKGTE
                     AGISVEVSFIERKKVETSLLQQNRVLNCSIAAPLYYWVQEGQVFYRLDGTEENRQSLL
                     QAVEAGRIRKVLVKYDVPFRTVASEEELLERLIQGFSLLEPYLKMTHLAK"
     gene            4399..5064
                     /locus_tag="AC1PNG_001163"
     CDS             4399..5064
                     /locus_tag="AC1PNG_001163"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIP6701671.1"
                     /translation="MEKLGQSLKWLGRKIWLLLRIFGLLVLAVLPDLILEGSKSQVTL
                     VIVGLVTLGLLVFFWWRAEKNNLPVWDKNVLSWDGLAIVILAFATMQLTDMLGFYLLE
                     LQGATTTANDASIIESLKGVPFGLALLTTAFLPAIAEEVILRGYFFKKLFGSQAVVGI
                     IVSSLLFGALHGPTDLASWLIYGGGGLIFCVLYHKTGYLIYPIAVHFINNAWSVVALY
                     YFQ"
     gene            complement(5144..6070)
                     /locus_tag="AC1PNG_001164"
     CDS             complement(5144..6070)
                     /locus_tag="AC1PNG_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIP6701672.1"
                     /translation="MNLSKEKLLFHHYYANWLRIYKEGAIREVTLKKYRMTLQWLIRL
                     APSLLLEDLNRVRYQELINSYAKTHERQTTMDFHHQLKGAILDAVDEGIIERDPTRKV
                     IIKGRLPRKKKTKYLNQFELHQLIQELRLGEELNWDWLILLIAKTGIRFSEALALTTQ
                     DFDFHRQLLTIDKTWNYKGDGGFLPTKNKSSIRKIQLDWQTSTQFANLLQHTQPDDLL
                     FVKKERIYNSTINAILEKRCLSANLPVVTIHALRHTHASILLFAGVSIASVARRLGHA
                     SITTTQKTYLHIIRELENQDVDLIMRSLSGLC"
     gene            6165..6767
                     /locus_tag="AC1PNG_001165"
     CDS             6165..6767
                     /locus_tag="AC1PNG_001165"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIP6701673.1"
                     /translation="MKNFKEREIMKLEELALFTGGSLQVRLDTTSWEDARDYILYNQQ
                     HHQLDGYEVIEEVIDSRTVTTDREVTLLEEGDLLFSLLSGKAVLVRAEHAGLLYTQNY
                     IKIEPIAKLDKAFLLYLINESSAIRRQFYQSLQGSEVMKYTVKQLKSLQLGALPPLDS
                     QQKLGKIYLDGLALRQKRQAYAQGDFLRLTYLLKEVYVHE"
     gene            6760..9966
                     /locus_tag="AC1PNG_001166"
     CDS             6760..9966
                     /locus_tag="AC1PNG_001166"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HIP6701674.1"
                     /translation="MNELAFETELIQYITTGTITQPEHLEGLEWSVSDSSTLYAVKSK
                     LWTYEPGIKTTEQLWENFRNILWQHNQHRLDKPLSDTEFAQVKQIISDLKTPYEAGQF
                     LYGISGVSELEITLDDGRHTFLTVFDQRKIGGGDTVYQVVNQIRRTPKIPGRRECIFD
                     TTLLINGLPIIQIEEKKDTVDVDKALNQMHQYIQERQYTDIFSTLQILVAIAPNNVRY
                     MANTTAEKFNKDFAFHWQRKSDGSKVRNWKEFADSMLSIPMAHQMATNYMILDGTPQK
                     QMLKVMRPYQVYATQAVIEGIKKVDFELGCNKVGYIWHTTGSGKTITSFKTAWLASRM
                     PKVDKVVFLVDRIALTNQTKDDYLAYDPEAGENSFGSIRETQNTTALARQLTSKDSGI
                     IVTSVQKLDTLVKRKSFKSPDKHIVFIVDEAHRSTGGDSFAGIQKAFKKAAWIGYTGT
                     PNLDDTGASLRTQDIFGPLLHAYTIKNAISDRNVLGFKVDFETTIDQEKLKTTYLPDF
                     YRQKYPKWTSAQIEEKIANLSPDDMDDAVTSSVYDENPEHVRLVVEDVFKHWANRSGS
                     GHYNALFTTHVGGGRASTPMAMMYFREFQRINQLHREQGGLVLKVGVTFALNTQNNDS
                     MVESNTGLLEAITIYNTEFGTSFGLSDVASYTEDLVSRLNRTAKDKNYLDLVIVVDQL
                     LTGFNAPELNTLYVDRTLKGASLIQAYSRTNRVEDMDKKPFGRVVNYRWPAHNEKLMN
                     QALAIYADDSAANLSEAEQKNNNEKNGLVAKPFKEVFAEVKQTVDQLRELTGDFNDIP
                     KSEKKREAMLELLRAYNQGLSKLKQYQPEEVDGVIEGFDYNQPDRFIESLGMSVEEEK
                     LLTTVLANELKRTLAKEQKVSIHQIELKMTHIKEVTIDYDKLTELVELLLNQVHEEKM
                     VEAAATRDELDRFASGLEDLNYADQIRQTGDAIYRGDFPTQDMNFAYPVKGLDSQKII
                     QEASRVYIDKKLLAFRIQWGLTEHIRNQELRELLARHRYGQNDLDDTGHLRTLIKEAS
                     RDYQTWAEGDAVKGLSKIQYRNQLRQALYDLADDLVGQ"
BASE COUNT         2891 a         2018 c         2481 g         2730 t
ORIGIN      
        1 attattctat gttaaaaagg agataggatt catcatgttt gagaagattt tgattgccaa
       61 tcgtggtgag attgcggtgc ggatcattcg tgcggccagg gaattaggga ttgcgacggt
      121 ggctgtctat tcagaggcgg acaaggaagc tctccacacc atgttggcag atgaggctgt
      181 ctgtatcggc ccggcccgtt cgacagactc ctatctcaat atgcaggctg ttatctcggc
      241 ggctgttgtg acaggtgccc aggctattca tcctggcttt ggatttttga gtgaaaactc
      301 taaattcgcc accctctgtg aagaggtcgg tatcaagttt atcggacctt ctgggacggt
      361 catggatacc atgggcgata aaatcaatgc tcgggcagag atgattaagg ctcaagttcc
      421 tgtcattcct ggatcagacg gcgaagtttt gaccagccaa gaagctcttg caattgctga
      481 aaagattggt taccctgtca tgctcaaagc atcggcagga ggtggcggta aggggattcg
      541 taaggtggaa aaagctgaag acctagtgcc tgcctttgaa tcagcttcca gcgaagccaa
      601 ggcagccttt ggcaacggag ccatgtatat ggagcgtgtc gtctatccag cccgccacat
      661 cgaagtacag attttggcgg accagcacgg tcatgtcatt catctgggag aacgggattg
      721 ttcccttcaa cgcaacaatc aaaaggtctt ggaagaagct ccgtcgattg ctatcggcca
      781 gaccatgcgg gaacgtatcg gtcaagcagc agtgcgggct gcccagtcag ttggttatga
      841 aaatgcgggg accattgagt ttctcttgga tgaagccaag ggcgaatttt acttcatgga
      901 aatgaacaca cgggtgcagg tagaacatcc tgttaccgaa tttgtcactg gtgtggatat
      961 tgtcaaggaa caaatcaaga ttgcggctgg tcaggaactc agtgtccgcc aagaagatgt
     1021 gcggattaca ggtcatgcca ttgaatgccg catcaatgct gaaaatccag ccttcaattt
     1081 tgccccaagt ccaggtaaga tttccaatct ctacctacca agcggtggcg ttggtctacg
     1141 tgtagattca gctgtttacc caggctacac cattccgcct tactatgatt ccatgattgc
     1201 caaggtcatc gtgcatgggg aaaatcgctt tgaagccctg atgaagatgc aacgggccct
     1261 ctatgaattg gaaattgacg gagtggtgac caataccgac ttccagctgg acttgatttc
     1321 cgacaagcga gtggtggctg gtgattatga cactgcctac ctcatggaag aatttttacc
     1381 ccgctatcaa gaagatctaa aaaagtaaac taggagaatg ctatggcttt gtttcgcaaa
     1441 aaggacaaat atattcgcat caatcccaat cgttcgcgga tagagtcagc acctcaagca
     1501 aagccagagg tgccagatga actcttttcg aaatgtcctg cttgtaagga aatcctctac
     1561 aagaaggact tgggaccaga gaaaacctgt caacactgtt cctataattt tcggattacg
     1621 gcccaggaac gcttggcttt gacagtggat gacggttcgt ttgaggaatt gtttacaggg
     1681 attgaaacca aaaatccctt ggacttcccc aattatttag agaaattagc agccacccgt
     1741 caaaaaacag gcttggatga agctgttttg acaggaaagg cgacaatcgg cggtcaacca
     1801 gttgcccttg gcatcatgga ttcgcacttt atcatggctt ctatgggcac ggtggtcggt
     1861 gagaaaatta cccgcctctt tgagttggcg attgaagagc gtttgccagt tgtcctcttt
     1921 accgcatctg gtggtgctcg catgcaggaa ggcattatga gtctgatgca aatggccaag
     1981 atttctgcag cagtgaaatg ccattccaat gctggcctct tttacctgac ggtcttgaca
     2041 gacccgacaa caggaggtgt tacggctagt tttgccatgg aaggcgatat tatcctggct
     2101 gagccgcaga ccttggttgg ttttgcagga cgacgggtta ttgagtcaac tgtgcgtgaa
     2161 aatctgcctg atgatttcca gaaggctgaa tttttacaag aacacggctt tgtcgatgcc
     2221 attgtcaaac gccaggactt gccagcgacc attggtcgct tgttgagaat gcatggaggt
     2281 gtcagatgac cagtgatgta gcacgtatga tccgtttggc acgtgaccaa gctcgcctga
     2341 cgaccttgga ctatgccaca cagctctttg atgactttat cgaattgcac ggcgatcgca
     2401 atttccgtga tgatggagca attgtcggtg gaattggcat tttagcaggt cagcctgtga
     2461 cagttatcgg tatccaaaaa gggaaaaatc ttcaggataa cctcaaacgc aactttggtc
     2521 agccccaccc agaaggctac cgcaaggccc ttcgcttcat gaaacaggcg gagaagttcg
     2581 ggcgtcccgt tgtcaccttt atcaatactg ctggagccta tcccggtgtc ggtgccgagg
     2641 agcgtggaca gggtgaggcc attgcccgca acctcatgga gatgagtgac ctcaaggtgc
     2701 ctattatcgc tattatcatc ggcgagggcg gctctggtgg agctctggct ctagcggtgg
     2761 cagatcaggt ctggatgctg gaaaactcca tgtacgccgt cctcagcccc gaagggttcg
     2821 cgtctatcct ctggaaagac ggcagtcggg ccatggaggc ggcagaactc atgaagatta
     2881 cctcctatga gttggagaag ctccaagtgg tcgataaagt tattttggaa aatggcagag
     2941 cgaccaagga agtcttggct gacatcaagg aaaacctggt cagtcagttg gctcaactac
     3001 aagccttgcc acttgataaa ttgctcgaaa accgctatca acgctttaga aaatactagg
     3061 aagacctagt atttttttgc tagatttgat acaatggata aaatcatttc aggaggtttt
     3121 ccatgttagt aaaagcagat ctatcaaacg cagaagaatt gctacctatt cagcaccgag
     3181 catttgcggc tttatataaa acctatcagg accagtacaa ccctgccatt gaaactatgg
     3241 actatttcca atcacgcttt gcacgaccaa attgtagcta ttacaagatt atcgaggaag
     3301 agcacacagt cggactagta cggacaacgg tcgctgaaga cgctgaacag ggctggctag
     3361 gcttgattgg tattgatcca gaccacagag gaaaaggcta tggtcacaag gctatgctcg
     3421 aattggaaag cctttatccg acagttagac gctgggttct ctgcacggtt ttgcaggagt
     3481 ctaaattagt ttctttctac gaaaagctcg gctacaaggc catcaaaaca gagcctgaac
     3541 aagaaggcat ggatatggtc tatatggaga agtggatagg agattcagat gcttgaacag
     3601 tgtcgcagat acctggatta tcaaggagtt cattattgca agcctgaaaa agcaggagct
     3661 ttggcaggcg atatgatagc cttaaaaaat gtagcacagg cagcacgcaa ggagtttcag
     3721 acattaagtt tagtcttagc tgagcacgtc agtccctttg tgcctgagag ggtcagtcaa
     3781 tggatgaatc aagcccaaat ttgcagacca catttttgga cctattaccg ttttccctct
     3841 gctagtctaa cggactcggc tcttgctatt cgtttgtatg gtaagggaac tgaagcgggg
     3901 atttcagtag aagtcagttt tattgaacga aagaaagtgg agacctctct gcttcagcaa
     3961 aatcgtgttc tgaactgctc gattgcggca ccgctttatt attgggtgca agagggacag
     4021 gtattttatc gcctggatgg aacggaggag aatagacaga gcctattaca agccgttgaa
     4081 gcaggacgga ttcgtaaagt attggtcaag tatgatgtac cgtttagaac cgttgcctcc
     4141 gaggaggaac tgctggaacg cttgattcag ggcttttctc tactagagcc ctacttgaag
     4201 atgacacacc tcgctaaata atcagtgggg tgatggcaac aaacatcgta agggtatttg
     4261 ttttttagtg tatattgaca aattcattga atttttgtaa aactgtatcg caacaacaga
     4321 acagtttgat tgacattacg tgaaatccta taatagattg aaggcactct atatgatgaa
     4381 gcggaagagg agaagagtat ggaaaaacta ggtcaaagtt tgaaatggct tggtcggaag
     4441 atttggctat tattaaggat ttttggtctc ttagtgcttg ctgtccttcc agatctgatt
     4501 ttggaagggt ccaaatcgca agtcaccttg gtaatagttg gactggtgac actgggtctt
     4561 ctggtctttt tctggtggcg ggctgagaaa aataatctac cagtctggga caaaaacgtt
     4621 ctttcttggg acggtctggc aatagtcata ctagcctttg ctaccatgca actgacagac
     4681 atgcttggtt tctatttgtt ggaattgcag ggggctacaa cgacagctaa cgacgcctcc
     4741 atcatcgaat ctttgaaggg agttcctttt gggctggcac tcttgacaac agcatttttg
     4801 ccagcaattg cagaagaggt tatattgcgg ggttatttct ttaagaaatt gtttgggtcg
     4861 caagcagttg ttggaatcat tgtttcaagc ctgcttttcg gagcccttca tggaccgacc
     4921 gatcttgcct cttggctgat ttatggaggt ggaggtctta tcttttgtgt tctctatcat
     4981 aagacgggct acttgatcta tcccatcgct gtccatttta tcaacaatgc ctggtcggta
     5041 gtggcactct actattttca ataagcaaca gaaaagacga ttccgagtgg aatcgtcttt
     5101 tgcaatgacc agtacaagct ttatcacgat agagatagga atgtcaacaa agtcctgaaa
     5161 gtgagcgcat aattaaatca acatcttggt tttccaactc acgaatgata tgaagatagg
     5221 ttttctgggt cgttgttatg cttgcatgac caagtcgtct agcaacactt gcgatagaga
     5281 ccccggcaaa taataaaata gatgcatggg tgtgtcgcag ggcgtgaatg gtgacgacag
     5341 gaagatttgc acttaggcac cgcttttcaa gaatggcatt gatggtagag ttgtaaatac
     5401 gttctttctt cacgaaaagt aggtcatcag gttgggtgtg ctgcaataag ttggcgaatt
     5461 gggtgctggt ttgccagtcg agttggattt ttcgaattga agatttattt tttgtaggta
     5521 aaaatccgcc gtctccttta taattccatg ttttatcaat ggtcaatagc tgtcggtgaa
     5581 aatcaaaatc ttgagtagtg agagcaaggg cttcggagaa tcgaatacct gttttagcaa
     5641 tcaataagat gagccaatcc caattcagct cctctccgag acgtaattct tgtatcaact
     5701 gatgaagttc aaattggttg aggtattttg tctttttctt tcgaggaagt cttcctttga
     5761 taatcacctt acgtgtcgga tctcgttcaa taattccttc atctacagca tctaaaatag
     5821 ctcctttcaa ctggtgatga aaatccatgg ttgtctgtcg ttcatgtgtt ttagcatagc
     5881 tgttaatcaa ttcttggtaa cgaacccgat tcaaatcttc caacaacaga ctaggagcta
     5941 gcctgataag ccactgcaat gtcattctat atttcttgag agtgacttct cgaatggctc
     6001 cttccttgta aatccgaagc caatttgcat aatagtgatg gaaaagtagt ttttcttttg
     6061 ataagttcat tgtagaacct ccttttttaa tttttgttaa ttacataatt tattgacaga
     6121 tttccgagga gatgatatac ttattttagc gaaaaaatgt aattatgaaa aatttcaaag
     6181 aaagggaaat tatgaaacta gaagaattgg ctctctttac gggaggtagt ttacaggttc
     6241 gcttggatac taccagctgg gaagatgcta gagactatat cctctacaat cagcaacatc
     6301 atcaattgga tggttatgag gtgatagaag aagtgataga tagtcggacc gtgacgacag
     6361 atagagaagt gaccttgcta gaggagggag acttactctt tagtttacta tcggggaagg
     6421 ctgttttagt gagagcagag cacgcaggct tactctatac acagaactat atcaaaattg
     6481 agcccattgc aaagctggat aaggccttct tactgtatct gatcaatgaa tcctcagcta
     6541 ttagacggca gttttaccaa agcttacagg ggagtgaggt aatgaaatat acagtcaagc
     6601 aactgaaaag tttacagcta ggcgccttgc ctcctttaga tagtcagcag aaactaggaa
     6661 agatttattt agatggatta gccttacggc agaagcggca agcttatgcg caaggagatt
     6721 ttttacgact aacatatttg ttaaaggagg tttacgtaca tgaatgaatt agcatttgaa
     6781 acagaattga ttcaatatat tacgactggg actattacac agccggagca cctagaaggt
     6841 ctagaatggt ctgttagtga ttcttcgacc ctgtatgcag tcaaatctaa actgtggact
     6901 tatgagccgg gtatcaagac aacggagcaa ttatgggaaa attttcgtaa tattctctgg
     6961 caacacaacc aacatcgatt ggacaaaccc ttgagcgata ccgagtttgc tcaggtcaag
     7021 cagattattt cagatctgaa gacaccttat gaggctggtc agtttttata tggaattagc
     7081 ggtgtttccg aattggaaat tacacttgat gatggacggc atacattttt gacagtattc
     7141 gaccaacgta agataggcgg tggggacact gtctaccaag tggtgaacca aatccgtagg
     7201 actcctaaaa tccctggacg acgagaatgt atattcgata caaccttgtt gattaatggc
     7261 ttacctatta ttcagattga agagaaaaag gatacggtag atgtggacaa ggcattgaac
     7321 caaatgcacc aatatatcca ggaacggcaa tatacggata ttttttctac cttgcagatt
     7381 ctagtcgcta tcgcccccaa caatgttcgc tatatggcca ataccactgc ggaaaaattt
     7441 aataaggact ttgctttcca ctggcagcgt aaatctgacg gtagtaaggt tcggaattgg
     7501 aaagaatttg cggattccat gctcagcatt cctatggccc atcagatggc gaccaactac
     7561 atgatcttag acgggacacc gcagaaacaa atgctgaaag tcatgcgtcc ctatcaagtt
     7621 tacgccactc aggcggtcat tgaggggata aaaaaagtcg attttgaact aggctgtaac
     7681 aaggttggct acatctggca tacgacaggc tcagggaaga cgatcactag ttttaaaaca
     7741 gcttggctgg caagtcgaat gcccaaggta gacaaggttg tctttctagt ggatcgtatc
     7801 gcattgacca atcagactaa ggatgattat ctggcttatg atccagaagc tggggagaat
     7861 agttttggca gtattcgaga aactcaaaat accacagcct tggcacggca gttgactagt
     7921 aaggatagtg ggattatcgt tacttctgtg caaaaattgg atacactggt gaagagaaaa
     7981 tccttcaaat caccagataa gcatatcgtg tttatcgttg acgaagctca tcgctcaact
     8041 ggtggagata gttttgcagg gattcaaaag gcctttaaga aagcggcttg gattggctat
     8101 acaggaactc ccaacttaga tgatacaggt gctagtctgc ggacgcaaga tatttttggc
     8161 cctcttcttc atgcctacac tattaaaaat gccattagtg accgcaatgt actagggttt
     8221 aaggtagatt ttgagacaac catcgatcag gaaaaactaa aaacaactta cttaccagat
     8281 ttttatcggc aaaaataccc gaagtggaca agtgcacaga ttgaagaaaa aattgccaat
     8341 ctaagtccag atgatatgga tgatgcggtg acttctagcg tctatgatga aaatccagag
     8401 catgttcgat tggtagtcga agatgtcttc aaacactggg caaatcgctc cggcagtggc
     8461 cattacaatg ccttgttcac cacccacgta ggtggaggca gggcctccac acccatggct
     8521 atgatgtatt tccgagagtt tcaacgaatc aatcaactgc atcgagagca aggaggtctg
     8581 gtcttgaagg tgggagtgac ctttgccttg aatactcaaa ataatgatag catggtcgag
     8641 tcaaatactg gtttgcttga agctattacg atctataaca cagaatttgg gacgtccttt
     8701 ggcttgtcag atgttgcttc ttatacagag gacctggtta gtcgcctcaa tagaacagcc
     8761 aaggacaaga actacttaga tttggtgatt gtcgtggatc agttgttgac aggtttcaat
     8821 gctcctgaac tcaatactct ttatgttgac cgaaccttaa aaggagccag tcttattcag
     8881 gcctattctc gtaccaatcg tgtggaggat atggataaaa aaccttttgg acgggtggtc
     8941 aattaccgct ggccagccca taatgagaaa ttgatgaatc aggccttggc catctatgca
     9001 gatgatagtg ctgccaattt atcagaagcg gaacaaaaaa ataacaatga aaaaaatgga
     9061 ctggtggcta agccttttaa ggaagtattt gcggaagtca aacaaacggt cgaccaattg
     9121 agagagttga cgggtgattt caatgatatt ccgaagtctg agaaaaagcg tgaggctatg
     9181 ttagaattgc tccgtgctta caatcaagga ctgtctaagc tcaaacagta tcagccagag
     9241 gaggttgatg gggtgattga aggatttgat tacaatcagc cggaccgctt tattgaaagt
     9301 ttgggaatgt ctgtagagga agaaaaactt ttgacgacag tcttagccaa tgaattgaaa
     9361 cgaaccttgg cgaaagagca aaaagttagc attcaccaaa ttgaactcaa gatgactcac
     9421 atcaaggaag tgacaattga ctacgataaa ttgaccgaac tggttgagct cttgctgaat
     9481 caggttcacg aagagaagat ggtagaagcc gcagctacca gggatgaatt ggatagattt
     9541 gcaagcggtt tggaagacct caactatgct gaccaaatcc gtcaaacagg ggatgcaatc
     9601 tatcgaggtg attttccgac acaagatatg aactttgctt atccagtcaa ggggcttgat
     9661 agtcagaaga ttatccaaga agcttcgcgt gtttatattg acaagaagct ccttgctttt
     9721 cgaatccaat ggggcttgac ggagcatatc cgcaaccaag aactacgaga gttgttggcg
     9781 agacatcgat atggtcagaa tgacttggat gataccggac acttacgtac cttgataaaa
     9841 gaagctagtc gcgattacca gacctgggca gaaggtgatg ctgttaaagg cttgagcaag
     9901 attcagtacc gcaatcagtt gagacaagca ctatatgatt tggctgacga cttggttggt
     9961 caataggttg cgattttgtt ggatgatagc gtataataga atggaaatcg tccgaaaaat
    10021 tgcaaaaata actttcagaa atagaacaaa aagagagtag aaaaggaggg actatgacaa
    10081 aagaaaaatc aaccgtacca cgattgcgct tccccggatt
//
LOCUS       DBNFEG010000070         9981 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.070, whole genome shotgun sequence.
ACCESSION   DBNFEG010000070 DBNFEG010000000
VERSION     DBNFEG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9981
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.070"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            10..300
                     /locus_tag="AC1PNG_001167"
     CDS             10..300
                     /locus_tag="AC1PNG_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HIP6701675.1"
                     /translation="MSRIKLTPDELRTSAIRYTEGSDNIDAILSSLSNEQSVIRENWE
                     GTAFDSFDAQFEALKPKIVEFSELLRDINAQLNKVADIIEQTDADIAAQING"
     gene            374..3523
                     /gene="esaA"
                     /locus_tag="AC1PNG_001168"
     CDS             374..3523
                     /gene="esaA"
                     /locus_tag="AC1PNG_001168"
                     /inference="COORDINATES: protein motif:HMM:TIGR03929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HIP6701676.1"
                     /translation="MDKQRIFKYGRNALLVLLLLLSIIGLNIAVQNNTSISKKEQEAG
                     QQNIKLDVALVNEDNTVTLNGTEYNLGASYAKNIEKNTAHNWSTVSRGTADKGLKEGH
                     YQLLVTIPSDFSTKILDINNTNVDKATITYKINANGNLQVENEAVKVAKGIIADLQSQ
                     LIDMYMASILGNLYQAQRNVQTLSDTKVTNIAVFRNNLYQTAVDFKTLFPNLTSTADS
                     TLAANNLLKEALSGNVTAYDSLNTAQTGLKDNLATLIEQRNQGKLSQEEFSASLLAMN
                     SSVLSAETDQLFATIKTTQEVLSGQLGAAAIDETGEASGYIGLVEKLDQAIADLQVAI
                     EAESTKLAEHEATISNFAETHLTTYYGDSLETIHIGAVLGKSTELPISLASYQTSLDQ
                     LILNQLAGLPSLTPADLTTDLNKLDPKAASQIRFDAAFAQAHFGEHFVASNDLKELQA
                     LAKELEPHLQAAADFKIPTPTTRATLSLEIPSGFDLVSWELNGNSYTNPSEVVALQKD
                     NTVTVTLQYEKSQTTTVSTTVVTDSTSTSSTTGSSSSEENTASTTAPSTGTPVTTTEM
                     AQGSVKVSVNNLEVTTAGFDWENFLKEQEAYRKAEVAYAAKVSEIISKYNQANRLLDQ
                     YYPVNTVTGERSSLTDSFFNQDAKSLLTSLVTSSITANLQSYSEARAQDQALKERLAE
                     LEGRKQELKDQLQYISTTNSDLAQQIANQLAFLEDVKARAQSIADGQEQQTALQGTHD
                     TELAALATELDASLSITGEVKSLSEQNVEEATNVNTVFTTFTNDVQAAQESGENLSSE
                     ATALMERFESELAENGDFVKTFTSVLSNTYDNGVPNEVILDFLSAPLTESSSSVKATV
                     NTYRPFTWILLLEMVALFAAYVFGTHRVIKAVKDKFKVSRLEDTDLVTVTVLSVLSLL
                     VGGLIGVVSSQQLQIDSELIPTWVLMITLFSLLLAQGQYLLIKNAKGLGMGISFFMVI
                     SFIYLSNAIGTTASLTGLPATLKAINPLSVLENSLSGYFDGEATSLTVIATVLVLGLG
                     FAALTAFVDVEKFLNKKED"
     gene            3524..3988
                     /gene="essA"
                     /locus_tag="AC1PNG_001169"
     CDS             3524..3988
                     /gene="essA"
                     /locus_tag="AC1PNG_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HIP6701677.1"
                     /translation="MKKIALVLSCLVLFLPVGIEASELGDNSLQIDSSRIEVEKEKKI
                     GSQDLLVQSLFLAPDQAELTAKKEADQVLLEHQLNSLFQTGEKPSTDILQVGTLFDTR
                     THKVMTTKVDSGTEGAPAGLLSGLFFASIVVGILLLASLATYLMGKDEVGQE"
     gene            4018..4257
                     /locus_tag="AC1PNG_001170"
     CDS             4018..4257
                     /locus_tag="AC1PNG_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HIP6701678.1"
                     /translation="METHINVTLHDGGRIRDLRIPTHIEVRRLIKELDSIFEKENARK
                     KYQLHVVNKGFILDEGKVLADYPVTTGDIVEVLEV"
     gene            4259..5521
                     /gene="essB"
                     /locus_tag="AC1PNG_001171"
     CDS             4259..5521
                     /gene="essB"
                     /locus_tag="AC1PNG_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HIP6701679.1"
                     /translation="MKEETFVFGGQEFVFLKGDKEWHLDVKRSDVATQELSDLLLLDL
                     HHPQFLQQEMTVDEDTVHFTYHLDAFGQTFEDMKEKEVSEKLRLSLNVLELKQALKLP
                     ITFFVHPSSVFITKDGRAKIAYRAIPEKMVPQTLDATDLLKQLKSYILALFTEHDFMD
                     LYNGALDVVPVPEFLDTIRKAEDMDSLEGLLQGYFQDKLEEEKATLTRVSRSKHRLYR
                     YATIWLSALAGLLVLPLIYLVFMHNPFKEKMLDADTAFIKVDYPGVISELERVEVSSL
                     PYTQKYTLAYAYIQGFDLSNEQRSVILNNVTLKSDEQYLDYWIEIGRGNSQEALDLAK
                     RLDDSDLILYALYQQMEQVRENTTLSGEERETTLDELKAEYQKYWDTRTTILNEDGSQ
                     VKTGTSSTSSSTSSTESSSTSPSSSSGE"
     gene            5525..9937
                     /gene="essC"
                     /locus_tag="AC1PNG_001172"
     CDS             5525..9937
                     /gene="essC"
                     /locus_tag="AC1PNG_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HIP6701680.1"
                     /translation="MAETVVFYKDGFRYAADVSAGKKILFQGTEKAQVFVSSLDTPLT
                     IWLDGVQVCYDYDGVEGNLVSGMSLAGGVVYLLPSEQVILDPLDKQELTIGQHVGNSF
                     VLAGSSCHVLLKRSDQSWMLYRLAGSIYINNLLMEKGEMELALGDELAFEDTFFKFYA
                     DEVLVAGPVEASDELARKSASRYAFYEDYPDYHRSPRIIYRSSEDRVAINAPSNAPSK
                     PSDSLLKLILPPLMMVGITLVIMIFQPRGLYVLATIAMSIVTLGMSIAGYIKGRKDYQ
                     KELRDREGLYHDYLADKAKELAGLTKSQKDGQLYHYPAIETLVDLADSYHHRIYEKTP
                     LHFDFLYYRLGLGEVPVSYDLSYAQTERSGKRDPLELEGFQLYEQNKTISDMPIVANL
                     SHGPVGYIGPRALVIEQLQLMVNQIALFHSYHDVQFITIMPEEEKEQWDWMRFLPHAT
                     LQDMNVRGFVYNQRTHDQVLNSLNQILKLRRAQKEDKSNRESTLFSPHYVVLVTDEKL
                     ILDHVIMEFFTEDPTDLGCSLVFVQDVLSSLSENIKTIINIKDRNTGQLVMEEGQLRE
                     IDFALDHFPVGYDKETLVRRLAPLNHLQNLKSSIPETVTFMEMYGAETFEDLGVVSRW
                     EKHAPYKSLAVPLGLRGKEDIVYLNLHEKAHGPHGLVAGTTGSGKSEVIQSYILSLAV
                     NFHPHDVAFLLIDYKGGGMANLFKDLPHLLGTITNLDGAQSMRALVSINAELKRRQRL
                     FATHDVNHINQYQKKYKLGEVSEPMPHLFLISDEFAELKTNQPDFMKELVSTARIGRS
                     LGIHLILATQKPSGVVDDQIWSNSRFKLALKVADRSDSMEMLKTPDAAEITQVGRGYL
                     QVGNNEVYELFQSAWSGADYQPDKDEQGIEDHTIYAINDIGQYEILNEDLSGLDQAES
                     IKEVPSELQAIVSKLKALTERLSIQALPQPWLPPLPSRLFVEDLRDEAELTWSGVNEE
                     LEVILGLLDKPSQQAQEPLSINLSQDGHLLMYGAPATGKTPFLQTTVLDLARHYSPSE
                     LHIYLMDFGTSGLVPMGDLPHVADVFLLDQGEKIGKFIRLLEKEINRRKKLLTEHRVG
                     TLSMYRQLTGQVEPRIVIALDSFESMKDESFEAELLKLLTRISREGQAIGLHLVVTAS
                     RQANLRATFYSNFKHQLTLKQHDKTEVRSVVGATGLADIEDIKGRALYKGEEVYSLQL
                     ALPLRVEHDIELVDQMREEVSRQKAAWTGQVPEAIPMVPEELTREDFLSRASVQKAIA
                     SGQIPLGLDLETVEAVSWDLTVGNFVYIYEQERHRNNFLRGFLNTLGQTDYSVKMVSG
                     EEFFTDNGTEVSFTPSSRLLMIDEIYDSVFENIEFDEYDEIVVVIWNELDVLVQEDSQ
                     LLDKMLYILTNGPKVGVYSLIVTTPGLSSKIDVVSKHVKLMKEAMVETRLNDQKILSL
                     NNVKYSEPSLQESLAYFIANKNYKLMKTVSEGSK"
BASE COUNT         2746 a         1938 c         2458 g         2839 t
ORIGIN      
        1 gagaatgata tgtcacgtat taaattaacc ccagatgagc tccgtacttc tgcgattcgc
       61 tatacagaag gttcagataa cattgatgct attttatcat ctttgtcaaa tgagcaatct
      121 gttattcgtg aaaactggga aggtacagct tttgacagtt ttgatgctca atttgaagcg
      181 cttaagccaa aaattgttga attttctgag ttgcttcgag atatcaatgc ccaattgaat
      241 aaagttgctg acattatcga gcaaacagat gcagacattg cagcgcaaat caatggctaa
      301 gggttgacga agttcaaatg ctaggaaggc gccagccttc ctatttttta tcgaaatgaa
      361 ggagatatga aaaatggata aacagcgcat ttttaagtat gggagaaatg ctttgctggt
      421 cttacttttg cttctatcta tcattggatt aaatattgct gttcagaaca acacgagtat
      481 ttccaagaaa gagcaagaag cagggcaaca aaatattaag ctggatgttg ccttggtcaa
      541 tgaagataat actgtaaccc taaatggtac ggaatataat cttggcgcta gttatgcaaa
      601 aaatatagaa aaaaatactg cccataactg gtcaactgtc agtcgtggta cggctgacaa
      661 gggcctaaaa gaaggtcatt accaattgtt ggtgaccatc ccaagcgatt tttcaacaaa
      721 aatcttggat atcaacaata ccaatgtgga taaagcgaca atcacgtata agattaatgc
      781 aaatggtaat ttgcaggtag agaatgaagc tgttaaggtg gcaaaaggaa ttattgcaga
      841 cctacagagt cagctgattg atatgtatat ggccagcatt ctcgggaatc tttatcaggc
      901 acaacgaaat gttcagacct tgtcagatac aaaggtgacc aatattgctg tttttaggaa
      961 caatctctat caaacagctg ttgattttaa gacacttttt ccgaatttga cttcgacggc
     1021 tgattcaaca ttggcagcta ataatctttt gaaagaagcg ctttctggca acgtgacggc
     1081 atatgatagc ctaaatactg ctcagacggg actaaaagat aatttggcta ccttgattga
     1141 acaacgaaat cagggaaaac tatcacagga agaattttca gcttcgcttt tagcaatgaa
     1201 tagctctgta ctcagtgctg aaacggatca gttgtttgca acgattaaaa cgacccaaga
     1261 ggtgctctcg gggcagttgg gggcggcagc gatagacgag acgggtgaag ccagtggcta
     1321 tattggtttg gttgaaaagt tggatcaagc tattgcagat ttacaggtag caattgaagc
     1381 tgagagtacg aaattagcag agcatgaggc tacgatttct aactttgctg aaacacattt
     1441 aaccacttac tatggcgata gtctggaaac aatccacatt ggagcggttt tgggtaagag
     1501 tacagagttg ccgatttctc tagcatccta tcaaacgagc ttagaccagt tgattttgaa
     1561 tcaattggca ggtttaccaa gcttgactcc agcagatctg actactgatt taaacaaatt
     1621 ggatccaaag gcagcgagtc agattcgatt tgacgcagca tttgcgcaag cgcattttgg
     1681 tgaacacttt gtagcttcta atgatttgaa agaattacag gctcttgcta aagagttaga
     1741 accgcatctt caagctgctg ctgattttaa aattcctact ccaacaactc gtgccactct
     1801 ttccttagaa attcctagcg ggtttgattt ggtatcttgg gaattgaatg gcaacagcta
     1861 taccaatcct agtgaagttg ttgctttgca aaaggataat acagtaaccg tcactctcca
     1921 atatgaaaag agtcagacga ccacggtttc aacaacggta gtaacggatt caacttcaac
     1981 ttcgtcaacg acgggttcga gctcatcgga ggagaatact gcatcgacta cggcaccgtc
     2041 cactggtaca cctgtcacga caactgagat ggcgcagggg agtgtcaagg taagtgttaa
     2101 taatctggaa gtcacaacag caggatttga ttgggaaaac ttcttaaaag aacaggaagc
     2161 ttatcgtaaa gcagaggttg cttatgctgc aaaagttagt gaaatcattt ccaagtataa
     2221 tcaagctaat cgtttattag atcaatatta tcctgttaat actgtaactg gtgaacgtag
     2281 cagtttgacg gatagtttct tcaaccaaga cgcgaaatcc ctgttgacct ctttagttac
     2341 gagctccatt acagctaatt tacaatccta ttctgaagcg cgtgcacaag atcaagcttt
     2401 gaaggaaaga ttggctgagt tagaaggacg caaacaagaa ttaaaagatc aactccaata
     2461 tatcagcacg acaaatagtg atttggctca acagatagcg aatcagctgg catttttgga
     2521 ggatgtcaag gcacgagctc agtctattgc agatggtcaa gagcaacaaa ctgctcttca
     2581 aggaacgcat gatacagaac ttgcagcgct cgctactgaa ttggatgctt ccctttctat
     2641 tacaggtgag gtaaaatccc tttcagaaca gaacgttgag gaggcgacta atgtcaatac
     2701 cgtcttcact acatttacta atgatgtaca ggctgctcaa gaaagcggag agaatctatc
     2761 ttctgaagca actgccctga tggagaggtt tgaatcagaa ttggctgaaa atggcgactt
     2821 tgtaaaaacc tttacttctg tcttaagcaa tacctatgac aatggtgttc caaatgaagt
     2881 gattttggat ttcttgtccg caccattgac agaatcgtct tcatctgtaa aagcaacagt
     2941 caacacctac agacctttca cctggatttt actcttggag atggtggccc tgtttgcagc
     3001 ttatgttttt ggtactcatc gtgtgattaa ggctgtgaaa gataagttta aagtcagccg
     3061 tctagaggat acagatttag taacggtgac tgtgctcagc gttctatcac ttctggtcgg
     3121 tggattgatc ggggttgtat caagtcaaca gttgcagatt gatagcgagt tgatcccgac
     3181 ttgggtgctg atgattacct tgtttagcct attactagca caaggtcagt atctattgat
     3241 aaaaaatgct aaaggacttg gcatggggat tagcttcttc atggttatca gctttattta
     3301 cctatctaat gccattggta caactgctag tttgacaggc ctgcctgcaa cactgaaagc
     3361 catcaaccct ctttctgttt tagaaaatag tttatctggt tactttgatg gcgaagctac
     3421 cagtctgaca gtgattgcta cggtgctcgt attggggctt ggttttgcgg ctttgactgc
     3481 ttttgttgat gtggaaaaat tcctaaataa aaaagaggat taaatgaaaa agatagcact
     3541 tgttcttagt tgtctggttc tctttcttcc ggttgggata gaagcgtccg aacttggaga
     3601 caatagtctt caaattgata gttcacgcat tgaagtagaa aaagaaaaga aaataggaag
     3661 tcaggattta ctggtccaat ctttgttttt agcgccagat caagcagaat tgactgccaa
     3721 aaaagaagct gatcaagtat tattagagca tcagctaaat agcctattcc aaactggtga
     3781 gaaaccaagc acggatattt tgcaagtggg gaccttgttt gatactcgga cccataaggt
     3841 gatgacaacc aaggtagata gtgggacaga gggggctcca gcagggcttc tctctggcct
     3901 gttttttgct tctattgtag ttggcattct attgcttgca agtttagcaa cttatttgat
     3961 gggtaaggat gaagtaggcc aggaataatc ttctagtggt ctattggagg agatgagatg
     4021 gagacgcata tcaatgttac cttacatgat ggaggacgaa tcagggactt acggattccg
     4081 actcacattg aggttaggag attaatcaag gagttggata gcatcttcga aaaagagaat
     4141 gctagaaaaa aataccaact ccatgttgtt aataaggggt tcattttgga cgaagggaaa
     4201 gtattagccg attatcctgt gacaacggga gatattgtag aggttttgga ggtttagggt
     4261 gaaagaagaa acatttgtct ttggtggaca ggaatttgtc tttttaaagg gtgataaaga
     4321 gtggcacctt gatgtaaaac ggtctgatgt ggcaacgcaa gagctatctg acctgctctt
     4381 gttggatctg caccaccctc aatttttaca acaggaaatg acggtggatg aagatacagt
     4441 tcatttcacc tatcatctgg atgcatttgg gcaaaccttt gaggatatga aggagaaaga
     4501 ggtttctgaa aaactacgac tttctctcaa tgttttagaa ttgaagcagg ctttgaaatt
     4561 gccaatcacc tttttcgtgc atccgtcatc tgtctttatt actaaggatg gtcgggccaa
     4621 aatagcctat cgggctatcc cggagaagat ggtgcctcag accttggatg ctacggattt
     4681 gttgaagcaa ttaaaatctt atatccttgc cttatttaca gagcatgatt ttatggatct
     4741 ttacaatggt gcgctggatg tggttcctgt accagagttc ttagatacca ttcgcaaggc
     4801 agaggatatg gatagtttgg aaggactttt gcagggctat tttcaggata agttggagga
     4861 ggagaaggcg actttgacaa gggtttctcg ctctaagcac cgtctctatc gctatgcgac
     4921 catctggctt tctgccctgg caggattatt ggtcttgcca ctgatttatc tagttttcat
     4981 gcacaatcct ttcaaggaaa aaatgctgga tgcggatacg gccttcatca aggtggacta
     5041 tcctggagtg attagtgagt tggagcgggt agaggtttct agtcttccct acactcaaaa
     5101 atatactctt gcctatgcct acatccaagg gtttgatttg agtaatgaac agcgttctgt
     5161 tatcttaaat aatgtaacgc tcaaatcgga tgagcaatac ttggactact ggattgaaat
     5221 tggtcgtggt aatagccaag aagctttgga tttggccaag cgtttggatg attcggattt
     5281 aattttgtat gctctttacc agcaaatgga acaggttcgt gaaaatacga ctttgtcggg
     5341 agaagagcgg gaaacaacct tggatgagtt gaaagcggag tatcaaaagt attgggatac
     5401 ccgaactacg attttgaatg aagatgggtc acaggtgaaa acaggtacaa gttcgacgtc
     5461 tagttcaacc tcttctacag agtcatcatc gacgagtcca tcgagctcga gcggggagta
     5521 agctatggca gagacagtag ttttttataa agatggtttt cgctatgcgg cggatgtgtc
     5581 tgctgggaaa aagattcttt ttcaaggcac agaaaaggca caggtttttg tctcctcact
     5641 ggatactcct ttgacgattt ggttagatgg agtacaagtt tgctatgact atgatggagt
     5701 ggaaggaaac cttgtatctg gtatgtcttt ggcggggggc gtggtctatc ttcttccgtc
     5761 ggagcaggtg atcctagacc ctcttgataa gcaggaatta acgatcggcc agcatgtggg
     5821 aaatagtttt gtccttgcag ggtcaagttg tcatgtcttg ctcaaacgca gtgaccagtc
     5881 atggatgctt tatcgtttgg cgggctccat ctatatcaat aaccttctga tggagaaagg
     5941 ggaaatggag ctggctcttg gagatgagct tgcttttgag gatacttttt tcaaatttta
     6001 tgcagatgag gtcctggttg cgggtcctgt agaggctagt gatgagttgg ctcgtaagtc
     6061 tgcatcgcgc tatgcttttt atgaggatta tccagactac catcgctctc cacggattat
     6121 ctatcgaagt tcagaagaca gggtggctat caacgcacct tcgaatgcac cttcaaaacc
     6181 gtccgacagt ttgttgaaac taatcttgcc tcctcttatg atggtgggga tcacattggt
     6241 tatcatgatt ttccaacctc gtggtctcta cgtgcttgcg actatcgcca tgtcgattgt
     6301 gacgttgggg atgtctattg ctggctatat aaaggggaga aaggattacc agaaagagct
     6361 gagagaccga gaggggctct atcatgacta tctggctgat aaggccaagg agttggctgg
     6421 tctgaccaag tcgcagaaag atggtcagct ttaccattat cctgctattg agaccttggt
     6481 ggatctggca gatagctacc accatcgaat ctatgagaag acgcctcttc attttgattt
     6541 cttgtattat cgcttgggtc tgggtgaggt tccggtgtct tatgacctgt cctatgctca
     6601 gacggaacgg tcgggcaagc gggatccctt ggagttggag ggttttcaac tctatgaaca
     6661 aaacaagacc atttcagata tgcccattgt ggccaatctg tcccatggtc ctgtcggcta
     6721 tattggtccg cgggctttgg tgattgagca gctccaactc atggtcaacc aaatagcact
     6781 tttccattcc tatcatgatg tgcagtttat taccatcatg ccagaggagg agaaggagca
     6841 gtgggattgg atgcggttct tgcctcatgc gaccttgcag gacatgaatg tccgtggctt
     6901 tgtctataac cagaggaccc atgatcaggt gctcaactcg ctcaatcaga tcttgaaact
     6961 gcgcagggct caaaaggaag acaagtccaa tcgggagtcg accctcttca gccctcacta
     7021 tgtggttttg gtgacggatg agaagttgat tttggaccat gtcatcatgg agttcttcac
     7081 agaggatccg acggatctgg gctgtagcct ggtctttgtg caggatgtgc tgtcttctct
     7141 gtctgagaat atcaagacca tcattaacat taaggatcga aacactggtc agctggtcat
     7201 ggaggaaggt cagttgcgtg agattgactt tgccttggac cacttcccag ttggctacga
     7261 caaggagacc ttggtgcgcc gcttagctcc gcttaatcac ctacagaacc ttaagtcttc
     7321 tatcccagaa acggtgacct ttatggagat gtacggggct gagacctttg aggacttggg
     7381 tgttgtcagt cgttgggaaa agcatgcgcc ttataagagt ttggcggtgc ctctgggtct
     7441 tcgtggcaag gaggatatcg tctatctgaa cttacatgaa aaggctcacg gtccgcacgg
     7501 tttggtggca ggtactacgg gttctggtaa gtcagaggtc atccagtctt atatcttaag
     7561 ccttgcggtt aatttccatc cgcatgatgt tgcctttctc ctcatcgact ataagggtgg
     7621 gggcatggct aacctcttca aggacctgcc tcacttactt gggaccatca ccaacttgga
     7681 tggcgcccag tccatgcggg ccctagtgtc catcaatgcg gagctgaagc gtcgtcaacg
     7741 tctctttgcg acccacgatg tcaatcatat caatcaatac cagaagaagt acaagctggg
     7801 tgaggtgtct gagcccatgc cccatctctt cttgatctcc gatgagtttg cggagctcaa
     7861 gaccaatcaa cctgacttca tgaaggagct ggtctcaacc gctcgtatcg gtcggagttt
     7921 gggcattcac ctgatcttag ctactcagaa gccatctggt gtggtggatg accagatctg
     7981 gtccaactcc cgcttcaagc tggcgctcaa ggtggcggat cggagcgact ccatggagat
     8041 gcttaagacg ccagatgcgg ctgagattac ccaggtgggt cgtggctacc tccaggtcgg
     8101 aaacaatgaa gtctatgaac tcttccagtc tgcctggtct ggggctgatt accagccaga
     8161 taaggatgag caggggattg aggaccatac catctatgct atcaatgaca ttggtcaata
     8221 tgagattctc aacgaagacc tgtcaggtct ggatcaggcg gagtccatca aggaggttcc
     8281 gagtgagctt caagccatcg taagtaagct gaaagccttg acagagcgcc tgtccatcca
     8341 ggccttgccg caaccatggt tgcctccctt gccttcgcgc ttgtttgtgg aagatttacg
     8401 agatgaggct gagttaactt ggtctggagt caatgaggag ttagaggtta ttctaggtct
     8461 cttggataaa ccgagccagc aggcccaaga accgctgtct atcaacctat ctcaggatgg
     8521 tcatttgctt atgtatgggg ctccagcgac cggtaagact cccttcttac agactacggt
     8581 cttggattta gctcgtcact atagcccatc tgaactccat atttatctga tggactttgg
     8641 gacgagtggt ctggttccaa tgggggattt accacatgtg gcggatgtct tcctcttgga
     8701 tcagggagag aagatcggta agtttatccg tcttttggag aaggaaatca accgtcgcaa
     8761 gaagctgctt acggaacatc gtgtgggtac cttgtccatg taccgtcagt tgacaggaca
     8821 ggtggagcca cgtattgtca tcgccttgga tagcttcgag tctatgaagg atgagagttt
     8881 tgaggcagag ttactcaaac tcttgacccg tatttctcgt gagggtcagg ccattggtct
     8941 tcaccttgtg gtgacggcta gtcgtcaggc taaccttcgt gcgaccttct actctaactt
     9001 caagcaccag ttgaccttga agcaacatga taagacagaa gtccgtagtg tggtcggggc
     9061 tactggtctt gcggatatag aggatattaa gggacgggcc ctctataaag gtgaggaggt
     9121 ttatagcctc cagttggcac ttccccttcg tgtggaacac gatatagagc tggtggatca
     9181 gatgcgtgag gaagtgagtc gtcagaaggc tgcttggacg ggtcaggttc cagaagctat
     9241 cccaatggtt ccagaagagc tgactcgcga ggacttcctc agtcgtgcca gcgtccagaa
     9301 agccatcgcc tctggtcaga ttccgcttgg tctggatctg gaaacggtgg aggctgtgag
     9361 ttgggatttg accgttggga actttgtata tatttatgaa caagagaggc atagaaataa
     9421 ctttttgcga ggttttttaa atactctggg acaaacagat tattctgtaa aaatggttag
     9481 tggcgaagag ttctttactg ataatgggac ggaagtttcg tttacacctt cgagtcgttt
     9541 attaatgatt gatgaaatat atgattctgt ttttgaaaat atcgaatttg atgaatatga
     9601 tgaaatagtt gttgtcattt ggaatgaatt agatgtttta gtacaagagg atagtcagtt
     9661 gttagataag atgctctata tattgactaa tggtcctaaa gtaggagtat acagtttaat
     9721 tgttactacg cctggcctat cttctaagat tgatgttgtt tctaagcatg ttaagttaat
     9781 gaaagaagct atggtggaaa cacgattgaa tgatcaaaaa attctaagct taaataatgt
     9841 taagtactca gagccgtcat tacaagagtc attagcctat tttatagcta acaaaaatta
     9901 taaattgatg aaaactgtga gcgaggggtc taaatgacaa ataatcaata taaaataaaa
     9961 attcatcata aagctgagaa c
//
LOCUS       DBNFEG010000071         9809 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.071, whole genome shotgun sequence.
ACCESSION   DBNFEG010000071 DBNFEG010000000
VERSION     DBNFEG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9809
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.071"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            32..2725
                     /locus_tag="AC1PNG_001173"
     CDS             32..2725
                     /locus_tag="AC1PNG_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH36-type glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HIP6701681.1"
                     /translation="MNKVEFIDQNGSFTLKQAQKNHTLYFPLASEKGLKSSITPLLGG
                     DSKISQNSFILEPVSIDNLHNNRSTRNVWTCIDGKETISLTGQSSDYDRDIAKEKQHD
                     QVTAGFMWHQLERTDEIANISTSITNFIPVSANIEVMHIRLKNLSNHPRRFSTYVAVP
                     IFGRSADNLRDHRHVTSLLHRIHTTKNGVVVNPTMTFDERGHHPNHLHYYVAGYTGEG
                     HTPVAFYPTVEEFIGESGNFTHPEAVFQHYPGYSDYKIDGREAMGAFRFEEVTLQSGQ
                     ETSYIVLLGITEEDTNIEEALADYSTDTQIQQHLEQTKQYWNNKVNVRFETGQADFDQ
                     WMKWICFQPYLRKIFGCSFLPHHDYGRGGRGWRDLWQDCLSLLLLEPTQVRRMIVNNF
                     KGVRIDGTNATIIGSGEGEFIADRNGITRVWMDHAFWPLLTTKLYIDQTGDTSILTEK
                     VSYFKDRQVKRGTAIDKEWKEENGSQLLTIDGNIYLGSILEHLLIEQLATFYDVGEHN
                     FYRLRDADWNDALDMASERGESVAFTCAYVGSMMDLAHLIRVYASQLENQTIQLPEEF
                     ETLLSQDLEVYDDYSKKQAVLQKFSEACNHKLSGKTIRVTFAELAVNLIEKANWLREQ
                     IQKTEWLSEDENGWFNSYYDNNGHQVETCHTNHPKMMLTGQVFSIMSYVANDQQIQSI
                     IRSADRHLYTPECGGYRLNTNFNEIKMDMGRMFGFAYGEKENGAVFSHMTTMYANALY
                     RRGYAKAGWKALKSLSNTALDYGTSRIYPGIPEYFRADGKGLYHYLTGAASWYLYTML
                     TEVFGFQGNLGNLILYPKLVAEQFNQQNLAQVTTTFAGKTFILTYHNPLRKEYGDYVI
                     SRAYINGIDLPISDDAYATLSKEKIEKLDSNSIHQITITLS"
     gene            2745..5156
                     /locus_tag="AC1PNG_001174"
     CDS             2745..5156
                     /locus_tag="AC1PNG_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH36-type glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HIP6701682.1"
                     /translation="MQYGYFDDKKKEYIITNPATPAPWVNYLGSPEYGAIISNNAGGY
                     SFATSGANGRILRYIFNHMDQPGRYIYIRDNDSKDYWSASWQPVGKDLLDYSIQCHHG
                     LGYTTMNAKYSSIATSTTYYVPKDQTYEVWAITLTNHSNQTRNLTVSGYAEFTNHGNY
                     EQDQVNLQYSLFISRTKFATNKIVQQLHGNLDSLKDEDQVDEKNVTERFFGLAGAQIS
                     SYCGDKQEFLGSYNGYHNPQGILSGHLGNILNTSENACGALSSQVILKPGQSKTIAFI
                     LGEKTQEEADRILHRFTNTEKEIKDDLLALKELWNSRLANLQVKTPSSEFNSMLNIWN
                     AYNCYMTFIWSRAASYIYCGLRNGYGYRDTVQDIQGIIHLHPEMAVDKIRFMLSAQVN
                     NGAGLPLVKFTHNAGHEDTPDDPSYVQETGHPAYRADDALWLFPTIYKYITETGNIDF
                     LDEVIPYANHGKATVYQHLQAAIDFSINHLGPHGLPAGLHADWNDCLRLGANGESSFV
                     ALQFYYALSIIKEFAVEKNDTAYLKQIEQLQEEYYSKIQHLCWDRDRFIRGFTENGEK
                     IGTPEANEANLWLNPQSWSVISGLANPDQAKLAMNQVFNRLNTQFGAILMDPPYHKEA
                     FDGALAVIYNQGTKENAGIFSQSQGWLILAEALLGNGNRAFNYYLENSPAAQNTIAEI
                     RKLEPYCFGQFTEGKDSPHFGRSHVHWLTGTASTMMVASVEGILGIRPTLDGIRLSPA
                     IPKEWPEVQITKQFRGKTLHITISNPHKKEAGCSSLILNGVEIDGNYIDQSLLKEENS
                     ILLTL"
     gene            complement(5261..5521)
                     /locus_tag="AC1PNG_001175"
     CDS             complement(5261..5521)
                     /locus_tag="AC1PNG_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIP6701683.1"
                     /translation="MSIELYKVRQGKIVSGVLAGIAHKLGWESWVTRAIFIASLLLGK
                     SFLLLIALYIAAAYFLPYKEDRDAERYGTGPRKIKEAEKIHK"
     gene            complement(5511..6272)
                     /locus_tag="AC1PNG_001176"
     CDS             complement(5511..6272)
                     /locus_tag="AC1PNG_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701684.1"
                     /translation="MTQKREERLIELYEKGVLTKEEARACFKEMNQEPDFLFVEEKVK
                     LNFTLPSFKVFASSKLKQSYSFEGIESLFLKLSEGRLTLAKSKNNQISVEICYNQDAP
                     EDKLPRLYVEKKGLYFHSSLACRLTINLPQEWMSVLDLELGQADARLDYLPFEDISIR
                     SSRDKKQQDIRLTTCGGYPQHLYVQLAQAPLTLQTGKGQGIRGQIESQSGHVLVNRKK
                     KASPYQFEKSGNDLLFLKVQTGQGGFYVKGIKDVN"
     gene            complement(6342..6776)
                     /locus_tag="AC1PNG_001177"
     CDS             complement(6342..6776)
                     /locus_tag="AC1PNG_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HIP6701685.1"
                     /translation="MDLNKYVQEVSLQDFGKEFRHVAIWNRRLRSTGGRFFPRDGHLD
                     FNPKHLEEQGLEVFRKIVRHELCHYHLYFEKKGYRHGDRDFKELLAAVDGLRYAPKLE
                     QAAKPSLIYRCQSCGQVYQRKRRIDLKKYRCGKCRGKLTLKE"
     gene            complement(6763..8919)
                     /locus_tag="AC1PNG_001178"
     CDS             complement(6763..8919)
                     /locus_tag="AC1PNG_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HIP6701686.1"
                     /translation="MEEKYMKIAQELGVSLKQIDTVLSLTAEGNTIPFIARYRKDVTG
                     NLDEVVIKSIIDRDKALTALADRKATVLAKIEEQGKLTDQLRQAIEEAEKLADVEELY
                     LPYKEKRRTKATVAREAGLFPLARLILQNVADLEEQAADFICEGFDTAQSCLAGAVDI
                     LVEAISEDNKLRAWVYHEVQTNSNLTSELKDQEADEKEVFQIYYDFSEKVAKMQGYKT
                     LAINRGEKLGVLKVSFEHNVDKMIRFFELRFPQSNSYIKDVIQQAIKKKILPAMERRI
                     RTELTEEAEEGAIQLFSKNLRNLLLVSPLKGKVVLGFDPAFRTGAKLAVVDQTGKLLT
                     TQVIYPVEPAGQRQIAQAKKDLADLIGQYQVEIIAIGNGTASRESEAFVADLLKDFPD
                     VSYVIVNESGASVYSASELARYEFPDLPVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVNQKSLSESLDFVVDTVVNQVGVNVNTASPALLAHVAGLNKTISENIVKYRE
                     ENGALTSRQQLKKVPRLGDKAFEQAAGFLRIPDATNFLDNTGVHPESYKAVENLLELL
                     AIDHLDEAAQEKLKQVTIADTAEKIGVGQETLKDIIADLLKPGRDLRDDFEAPVLRQD
                     VLDVKDLVVGQELQGTVRNIVDFGAFVDIGVHEDGLVHISRMVKRKRDKNGRQQALPH
                     PSEILAVGEIVTVWVVEVDIKRNRIGLSLLKPNGSE"
     gene            complement(8980..>9809)
                     /locus_tag="AC1PNG_001179"
     CDS             complement(8980..>9809)
                     /locus_tag="AC1PNG_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIP6701687.1"
                     /translation="YIFLILVGILILLYPYISSSWNRYRDQQLISSHREWVDTQPEQE
                     KEKMWQVAQSYNNELGIQPVPDAFSLREGIHDPKYETVLNASQDGMMGYVEIPKIDIE
                     LPIYHYTSDSVLQKGAGHLFGSALPVGGDGSHTVVSAHRGLPNAEMFTNLPQLEKGDQ
                     FYFHVLGEILAYEVDWIQVVKPNQVSSLSGIEGEDYATLITCTPYGINTERILVRGKR
                     VPYDKKDYEKAQSSQKKVDEIYLAVQVVCGVLGFLLSLLIILFVKKRDQKRKNKESM"
BASE COUNT         2988 a         2146 c         1902 g         2773 t
ORIGIN      
        1 tcttaacgac ctatattcag aaaggattct tatgaacaaa gtagaattta ttgatcaaaa
       61 tggtagcttt acattgaaac aggctcaaaa aaatcacaca ctttattttc ctcttgcatc
      121 tgaaaaagga ttaaaaagtt ccatcacacc attattgggt ggagattcaa aaattagcca
      181 aaatagcttt attttagaac cggttagtat tgacaatctc cataataacc gctccacccg
      241 taatgtatgg acctgcattg atggaaaaga aacgatttcc ttaactggac agtcttctga
      301 ttacgataga gacattgcta aagaaaagca acacgaccaa gtgacggctg gatttatgtg
      361 gcatcaatta gaacgtacgg atgagattgc taacatatca actagtatta caaatttcat
      421 accagtttca gccaatattg aagtcatgca tattcgattg aaaaatcttt ccaatcatcc
      481 gagacgattc tctacttatg tggccgttcc tatttttgga aggagtgctg acaatcttcg
      541 tgatcaccgc catgtaactt cattattgca tcgtattcac acaacaaaaa atggggttgt
      601 tgtcaatccg accatgacct ttgacgaacg gggacatcac ccaaatcatt tgcactatta
      661 tgtagctggt tatacaggtg aagggcacac cccagtagca ttttacccaa ctgtagagga
      721 atttattgga gaaagtggta actttactca tcccgaagct gtttttcaac actatcccgg
      781 ttattctgat tataaaatag atggaagaga agcgatggga gcctttcgct ttgaagaggt
      841 tactctccaa tctgggcaag aaacttccta tattgttttg ttaggaatta cagaggaaga
      901 tacaaatatt gaagaggctt tagctgatta ctcaactgat acacaaattc aacaacattt
      961 agaacaaact aagcagtatt ggaacaacaa agtgaatgtg cgatttgaaa caggccaagc
     1021 tgactttgat caatggatga aatggatttg tttccaacca tacttgagaa aaatttttgg
     1081 atgttcattc ctcccgcacc atgactatgg aagaggaggt cgaggttgga gagacctctg
     1141 gcaagattgt ctctcactgt tactattaga acctacacaa gttcgacgta tgatagtcaa
     1201 taacttcaaa ggtgttcgaa tagatggaac aaatgcgacg ataattggct cgggagaagg
     1261 agaatttatt gcggaccgaa atggtattac ccgtgtttgg atggatcatg ctttttggcc
     1321 actgcttact acaaaactct atattgatca aacaggagat acatctattc tgactgaaaa
     1381 agtttcctat ttcaaagaca gacaagtaaa acgtggcaca gccattgaca aagaatggaa
     1441 ggaagaaaat ggttcacagt tgcttactat agatggaaat atttacttag ggagcattct
     1501 agaacattta ctgattgaac aattggctac gttttatgat gtcggtgaac acaattttta
     1561 tcgattacgt gatgcagatt ggaatgatgc tttggatatg gctagcgaaa gaggggaaag
     1621 tgtggcattt acctgtgcct acgttggcag tatgatggat ttagctcatc ttattcgagt
     1681 ttatgcaagc cagctagaga atcaaacaat acaattacca gaagagtttg aaacactgct
     1741 ttctcaagac ctggaagttt atgatgatta ttcaaaaaaa caagctgtac tacaaaaatt
     1801 ctctgaagct tgcaatcaca aattaagcgg gaaaaccatc cgtgttacat ttgccgaact
     1861 cgcagttaat ctgattgaaa aagcaaattg gttgcgagaa caaattcaaa aaaccgaatg
     1921 gttaagtgaa gatgagaatg gatggtttaa cagttattat gataataatg gccatcaagt
     1981 agagacttgc cacaccaatc atccaaaaat gatgttgact ggacaggtct tctctatcat
     2041 gagctacgta gcaaatgacc aacagattca gtctatcatt cgtagcgcag atcgccattt
     2101 gtatactcct gaatgcggtg gttacagact gaacacaaac tttaatgaaa taaaaatgga
     2161 tatgggacgg atgtttggtt ttgcatatgg agaaaaagaa aacggagctg ttttttctca
     2221 tatgacgact atgtatgcta acgctttata tcgtcgtggc tatgcgaaag ctggatggaa
     2281 agcattaaaa tctttatcaa atactgcatt ggattatggc actagccgaa tctatccagg
     2341 aattccagaa tatttccgag cagatggaaa aggcctctat cactacctga ctggagctgc
     2401 aagctggtat ttgtatacta tgctaacaga ggtgtttggt tttcaaggaa atcttgggaa
     2461 tctcatcctc tatccaaaac ttgttgctga acaatttaat caacagaacc tagcccaagt
     2521 aacaacaact tttgctggca aaacctttat cctaacctat cacaatccat taagaaaaga
     2581 atatggtgac tatgttatca gtcgggccta tatcaatgga atagaccttc caatttcaga
     2641 tgatgcctac gccactttat caaaagaaaa gattgagaaa ttggattcca acagcatcca
     2701 tcaaatcacc attacactct cttgaagact cgaaaggaaa tattatgcaa tacggatact
     2761 ttgatgataa gaaaaaagaa tatattatta ccaaccctgc tactccagcc ccttgggtca
     2821 attatctagg ttctccagaa tatggtgcaa tcatttcaaa caatgctggt ggctatagtt
     2881 ttgcaacgtc tggtgcaaat gggcggattc tgcgatatat atttaatcat atggaccaac
     2941 caggacgtta tatctacata agagacaatg atagcaaaga ctattggtct gcatcctggc
     3001 agcctgttgg aaaagatttg ctagattact cgattcaatg tcaccatgga ttaggctata
     3061 caactatgaa tgcaaaatac agtagcattg caacgagtac cacctattat gttcctaaag
     3121 atcaaaccta cgaagtatgg gccataacct taaccaatca ttcaaaccaa acaagaaatc
     3181 tcactgttag tggatatgct gaatttacaa atcatgggaa ttacgaacag gatcaagtaa
     3241 atctacaata ctctcttttt attagtcgaa caaaatttgc gaccaataaa atagttcaac
     3301 aactccatgg aaaccttgat tccttgaaag atgaagatca agttgatgaa aaaaatgtca
     3361 ctgaaagatt ctttggatta gctggcgctc aaatatcatc ttactgcggc gataaacaag
     3421 aattcttagg atcctataat ggctatcaca atccacaagg aatcctttcg ggacatctag
     3481 gaaacattct taataccagc gaaaatgcct gcggtgcctt atctagtcaa gtgattttaa
     3541 aaccaggcca atctaaaaca atagctttca tccttggaga aaaaacgcaa gaggaagcag
     3601 atcgaatact ccatcgattc actaacactg aaaaggaaat caaagatgat ctcctcgcat
     3661 taaaagaact ttggaatagt cgattagcaa atttacaagt taaaacacct agctcagaat
     3721 tcaattcaat gcttaacatt tggaatgcct ataactgtta catgaccttt atctggtcac
     3781 gcgcagcttc ctatatctac tgtggattgc gtaatggcta tggctatcga gatactgttc
     3841 aagatataca aggtataatc cacctacacc ctgaaatggc cgttgataaa ataaggttta
     3901 tgctgtccgc ccaagtaaac aatggagctg gccttccact tgtaaaattt acccataatg
     3961 ctggacacga agatactcca gatgaccctt catatgttca agaaacggga caccctgcat
     4021 accgagccga tgatgctctt tggctcttcc cgaccattta taaatacata actgaaacag
     4081 ggaatattga ctttctggat gaagttattc catacgcaaa tcatggaaaa gcgacagtat
     4141 accaacatct tcaagcagct attgactttt caataaatca tctgggtcca catggattac
     4201 cagctggact acacgctgac tggaacgatt gcttgcgact tggagcaaat ggcgaatctt
     4261 catttgttgc gctacaattc tattatgccc tttctatcat caaagaattt gcggttgaaa
     4321 agaatgatac agcttattta aagcaaatag aacaacttca agaggaatac tattcgaaaa
     4381 ttcaacatct atgctgggat agagacagat ttatccgtgg cttcactgag aatggggaaa
     4441 aaatagggac tcctgaagca aatgaagcta atctttggtt aaatcctcag agttggtcag
     4501 ttattagtgg tttagcgaac ccagatcaag cgaaactagc tatgaatcaa gtttttaatc
     4561 gcttaaatac acaatttggt gctattttga tggatcctcc atatcataag gaagcctttg
     4621 atggagcact tgcagtcatc tataatcaag ggacaaaaga aaatgctggc atcttttccc
     4681 aatcacaagg ttggctcatc ctagcggaag ccttattggg aaacggcaat cgagctttca
     4741 actattattt agaaaactct ccagccgctc aaaatactat agctgagatt cgtaaactag
     4801 aaccttactg ttttggtcaa tttacagaag gcaaagacag tcctcacttt ggtagatcac
     4861 acgttcactg gctaacaggt actgcttcaa ccatgatggt tgcgagtgta gagggaattc
     4921 taggaattcg accaacactt gacggtattc gcttatcacc tgccattcca aaagaatggc
     4981 ctgaggtaca aattactaaa cagtttcgag gaaagacgct acatattaca atctctaatc
     5041 ctcataaaaa agaagctggc tgtagctctc taatcttaaa tggagtagaa attgatggaa
     5101 actacattga ccaatcacta ctgaaagagg aaaattcaat tttattaacc ctctaattca
     5161 aacttcctcc ctccccactc tttaagagaa tggggagttt tattatccag atagcaaaaa
     5221 gaaaaagacc atcaaacgat aatcttttac aaatacaatg ttacttatgt attttttccg
     5281 cttcttttat cttacgtgga cctgttccat agcgttccgc gtctctatct tccttataag
     5341 gtaaaaaata agctgctgca atatagagag caatcagaag gaggaaggat ttgcctaaga
     5401 ggagacttgc aataaatatc gcgcgtgtta cccaggattc ccagcccaat ttatgcgcta
     5461 tgccggcaag aacccctgat actatcttcc cctgccttac tttatataat tcaattgaca
     5521 tcttttattc ctttcacgta aaagcctcct tgacctgttt gtaccttcaa gaaaagtaaa
     5581 tcattgccag atttttcaaa ctggtaggga ctggctttct tttttcggtt gaccaagaca
     5641 tggcctgatt gcgactcaat ctgcccccga attccctggc ctttgccagt ttgtaaagtc
     5701 aagggagcct gagctagttg cacatacaag tgttggggat aaccaccgca agtcgtcaag
     5761 cgaatatcct gttgtttttt atcccttgaa ctgcggatag aaatatcttc aaacggtaag
     5821 taatccaagc gagcatcggc ctgccccaac tccaagtcca atacggacat ccattcctgt
     5881 ggcaagttga ttgtcaaacg acaggccaag ctactgtgaa aataaagccc ctttttttca
     5941 acataaagac gaggaagctt atcttctggt gcatcttgat tgtagcaaat ctccacacta
     6001 atctgattat ttttagactt agccaaagtt agacgtccct cagataattt taaaaagagt
     6061 gattcaatcc cttcaaaact ataggattgt ttcaatttgg aggaagcaaa gaccttaaaa
     6121 cttggcaagg taaaattcaa ttttactttt tcttccacaa aaagaaagtc tggttcttga
     6181 ttcatttcct taaaacaggc acgcgcctct tccttggtca agacaccttt ttcatataac
     6241 tcaattaagc gttcttctct cttttgcgtc atagactcac ctcaattcta ctctagtata
     6301 tcacatttct ccacaaaaaa atcagcccga agactgattc tctattcttt caaagtaagt
     6361 ttgcccctac acttcccaca tcggtatttt tttaggtcaa tgcggcgttt gcgctggtag
     6421 acttgtccac aggactgaca tctataaatc aggctaggct tggcagcttg ttccagtttg
     6481 ggagcatagc gcaggccgtc caccgcagcc aacaattcct tgaaatccct gtctccgtgc
     6541 cgatagcctt tcttctcaaa gtagagatgg tagtggcaga gctcgtggcg gacaatcttc
     6601 cgaaagactt ctaaaccttg ctcctccaag tgcttgggat tgaaatccaa atgcccatcc
     6661 cttggaaaaa agcgaccacc cgttgagcgt aatcgcctgt tccagattgc tacatgccga
     6721 aattccttac cgaagtcttg caaggagact tcttgcacat atttattcag atccatttgg
     6781 tttcaaaaga ctaaggccaa tacgatttcg tttgatgtcc acttcaacca cccagaccgt
     6841 tacgatttcc ccgacggcga ggatttcact tggatgaggc aaggcttgtt ggcgtccatt
     6901 cttatcccgt ttgcgtttga ccatgcgtga aatgtgaacc agaccatcct cgtggacacc
     6961 gatgtccaca aaggctccaa agtccacaat attgcggacc gtaccttgca attcctgtcc
     7021 gactaccagg tccttgacat ccaagacatc ttgacggagg actggtgctt caaagtcatc
     7081 acgtaaatca cgacctggct tgagcaagtc tgcgataatg tccttcaagg tttcttgacc
     7141 gacaccaatt ttttctgccg tatctgcgat ggtcacctgc ttcaattttt cctgcgcagc
     7201 ctcatctaag tggtcaatcg ctagaagttc taacaggttt tccacagcct tgtaagactc
     7261 ggggtgcacg ccagtattgt ccaaaaagtt agtcgcatca ggaatccgca agaaaccagc
     7321 tgcctgctca aaggccttgt cacccagacg aggcaccttt ttgagttgct gacgagaggt
     7381 cagagcacca ttttcctcac ggtacttaac gatgttctcc gaaatggtct tgttaagacc
     7441 agccacgtga gccaaaaggg caggactggc cgtattgaca ttgacaccga cctggttgac
     7501 caccgtatcg accacaaagt ccagactttc agacagagac ttctggttga catcgtgctg
     7561 gtactggccg acaccgatag acttgggatc gattttgacc agctcagcca gagggtcttg
     7621 cagacggcgg gcgatggaaa tggcagagcg tttttccaca ggtaggtctg gaaactcgta
     7681 tcgcgccagt tcagaagctg agtagacaga agctccgctt tcattgacga tgacgtaaga
     7741 aacatcaggg aagtccttga gtaaatcagc tacgaaggcc tcagactcac ggctggccgt
     7801 tccatttccg atagcaatga tttccacttg gtattgtcca atcaggtctg ccaagtcttt
     7861 ttttgcctga gcaatttgtc gctgaccagc cggctccaca ggatagatga cttgtgtagt
     7921 caagagtttt ccagtctggt ctaccactgc tagcttggcc cctgtccgaa aggctgggtc
     7981 aaaaccaaga acaaccttgc cttttagagg agatacgaga agtagattgc ggaggttttt
     8041 agagaagagc tggatagctc cttcttcggc ttcttctgtc aattctgttc gaatacggcg
     8101 ctccatggca ggcagaattt tcttcttaat agcctgctga atgacatcct tgatgtagct
     8161 attagactgt ggaaaacgca actcaaagaa gcggatcatt ttatccacat tgtgttcaaa
     8221 agaaaccttg agaacaccta atttctcacc acggttaatg gccagggtct tatagccctg
     8281 catcttcgct actttctcag aaaagtcata gtaaatctga aagacttcct tctcatctgc
     8341 ttcttgatct tttagctctg aagtcaggtt tgaattggtt tgtacttcat gataaaccca
     8401 tgcccgaagc ttattgtctt ctgagatagc ttccaccaaa atatccacag caccagccaa
     8461 acaagactga gcagtgtcaa agccctcgca gataaagtcc gcagcctgtt cttccaagtc
     8521 agccacattt tgcaaaatca atcgagccag cgggaacagt ccagcctcac gcgccaccgt
     8581 tgccttggtc cgacgttttt ccttataagg caggtagagt tcttccacat ctgccaactt
     8641 ttccgcttcc tcgatagcct gtcgaagctg gtctgtcaat tttccctgtt cttcaatctt
     8701 ggcaaggaca gtagccttac ggtcagcgag agcagtcaag gccttatcac gatcaatgat
     8761 agacttgatg accacctcgt ccaagttccc cgtcacatcc ttccgataac gagcgataaa
     8821 gggaatggtg ttgccttcag ccgtcaaaga gagaaccgta tcaatctgtt ttaaactaac
     8881 gcccaattcc tgagcaattt tcatgtattt ttcttccata gctactatta tagcatgaaa
     8941 gcggtacaaa tgactcaata gaaaaagcac gtagataaac tacatgctct ctttattttt
     9001 tctcttctga tcacgttttt tcacaaataa aatgattagg agagatagga ggaatcctaa
     9061 tacaccacaa accacttgaa cagcaaggta aatctcatca actttctttt gactagattg
     9121 ggctttttcg taatccttct tatcatacgg aactcgtttc ccgcgaacca aaatccgttc
     9181 tgtatttatt ccataaggtg tacaagtgat caatgtcgca taatcttctc cctcaatacc
     9241 ggacagtgaa cttacctgat tcggcttcac aacttgaatc caatcaactt cgtaagcaag
     9301 aatctctccc aaaacatgga aatagaattg atctcctttt tctaattgcg gaagattggt
     9361 aaacatttct gcattcggta accctctatg ggctgataca accgtatgac taccatctcc
     9421 cccaacgggc agggcactac caaataggtg accagctcct ttttgtaaga cactgtcgct
     9481 tgtatagtga tagatgggta attcaatatc gatttttgga atttctacat accccatcat
     9541 cccatcctga gaggcattta acactgtctc atacttagga tcatgaatcc cctcacgtaa
     9601 ggaaaaggca tcaggtacag gctgaatccc taactcatta ttataggatt gagcaacttg
     9661 ccacattttt tccttttctt gttctggctg agtatctacc cactctctat gagaactgat
     9721 taattgttga tcacggtacc gattccaact gctactgata tacggataca ataaaatcag
     9781 tatccccact aaaataagga aaatatatc
//
LOCUS       DBNFEG010000072         9761 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.072, whole genome shotgun sequence.
ACCESSION   DBNFEG010000072 DBNFEG010000000
VERSION     DBNFEG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9761
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.072"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(116..454)
                     /locus_tag="AC1PNG_001180"
     CDS             complement(116..454)
                     /locus_tag="AC1PNG_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HIP6701688.1"
                     /translation="MSTNIYDIANELERAIRNLPEYKAVEAVKVSVEGNSEAKEILES
                     YISFQKEIQSKLQAGEIPTEADQKKMLDFNKKVQGNPLLTEYFSKQQQLGTYVADLER
                     IIFKPLNELL"
     gene            complement(464..1567)
                     /locus_tag="AC1PNG_001181"
     CDS             complement(464..1567)
                     /locus_tag="AC1PNG_001181"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIP6701689.1"
                     /translation="MKKTILIVGLGLIGSSLALCIRKDHPDYHLIGFDPNEQSCQIAL
                     GKGMVDEVSTELEGVAKQADVILLCVPIQVSLRLIEEFSALELKDTVLITDAGSTKSA
                     IVAAAEEHLAPRQINFIGGHPMAGSHKSGASAADLHLFENAYYIMTPCQATKQDAVPR
                     LTDLLSGTGARFVQIDAAEHDRVTSQISHFPHVLASSLMHQAGEYAQEHSFTNNFAAG
                     GFRDMTRIAESEPSMWTSILLTNADSILNRIEEFQERLSEISDVLRSGDQKAIWDFFD
                     KGRQTRRAMEIHKRAGVDSFYDLFLSVPDEEDTILKVLEVLRGISIVNIRINEENRED
                     IHGILQITFKNKGNLEDAAKRLREQTTYKIHLD"
     gene            complement(1575..2741)
                     /gene="aroC"
                     /locus_tag="AC1PNG_001182"
     CDS             complement(1575..2741)
                     /gene="aroC"
                     /locus_tag="AC1PNG_001182"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIP6701690.1"
                     /translation="MRYLTAGESHGPRLTAIIEGVPAGLPLTAEDINEELKRRQGGYG
                     RGSRMQIETDRVEITAGVRHGKTTGAPITLNVTNKDHQKWLDIMAVEDIEEKLKSKRR
                     IKHPRPGHADLVGGMKYRFDDLRNSLERSSARETTMRVAVGAVAKRILAELDIEIANH
                     VVVFGGKEIDVPDGLSVAEIKERAAQSEVSIVNQEREEEIKAYIDQIKRDGDTIGGVI
                     ETVVGGVPAGLGSYVQWDKKLDAKLAQAVVSINAFKGVEFGVGFQAGYLKGSQVMDEI
                     LWSQEKGYTRRTNNLGGFEGGMTNGEALIIRGVMKPIPTLYKPLMSVDIDTHEPYKAT
                     VERSDPTALPAAGVVMESVVATTLATEILEKFSSDNMEELKAAVASHRDYVKNF"
     gene            complement(2745..3419)
                     /gene="aroD"
                     /locus_tag="AC1PNG_001183"
     CDS             complement(2745..3419)
                     /gene="aroD"
                     /locus_tag="AC1PNG_001183"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HIP6701691.1"
                     /translation="MKIVVPIMPRNLEEVETIDVERLAEADIVEWRADYLPKDDILRV
                     APAIFEKCSGKEVVFTIRTTREGGHLDLDDQEYVNVIKEVATLYQPDYIDFEYYSYKS
                     VFEQMLEFPNLVLSYHNFEETPSNYMEIMSELTSLSPAVVKMAVMAKTEQDVLDVMNY
                     TRGFKSLNTEQIFATIAMGELGKLTRIAGVITGSCWTFASLDETSAPGQMSLSNTRKF
                     LEILEN"
     gene            complement(3416..4579)
                     /locus_tag="AC1PNG_001184"
     CDS             complement(3416..4579)
                     /locus_tag="AC1PNG_001184"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIP6701692.1"
                     /translation="MKIKVNRIAEQKINRGIQLLDSADFSGVSFDEDRLAVLYSESGS
                     YLGQAYLSKQNKGIGWLFSRSNQDLTMDFFVKLFVDAKQKRQHFYQSEMTNAFRLFNQ
                     DGDSFGGMTVDLYDEYAVFSWYNTFIYSVKEMIVAAFQQVFPEVRGAYEKIRFKGLAF
                     ESAHLYGEEAPEYFTVLENGVHYQVFMNDGLMTGIFLDQHEVRGSLVDGLAAGKSLLN
                     MFSYTAAFSIAAAMGGAEETTSVDLAKRSRELSKAHFKVNSLDLDNHHFVVMDVFEYF
                     KYAKRKGLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNTGGTIIASTNAA
                     NVSVEKFKRQIEKGFDVRKHRYEATYRLPADFVINKNDESSNYLKVFTIQVDK"
     gene            4695..6869
                     /locus_tag="AC1PNG_001185"
     CDS             4695..6869
                     /locus_tag="AC1PNG_001185"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIP6701693.1"
                     /translation="MKKLIDYVKKSGQTRLGFVLILLIIYWLKTLWAYYVDFNLDTQG
                     FFQNFIAIMNPLPVGILLLGLSLYIKNTKVFYSVAMIIYIILSVWLFSNAVYYREFSD
                     YISISTIMATSSVSAGLGEAALKLFRLWDIVYFLDFIIFGILVYRKLLRWDKKTFHKK
                     SSFAITALSVMLFSINLFLAEIDRPELLTRGFSNTYIVRALGLPAFLGYNANQTYNAH
                     KNRSGASATDLEPVKQYITEHYAQPNDNYFGIAKGRNVIYLHLESLQQFVIDYKLNVN
                     GVEYEVTPFLNSLYHSSSTLAFSNFFNQVKAGKTSDAETMIETSLFGLNQGSFMVQYG
                     GSNTQQAAPYILSQNGGYTSAVFHGNAASFWNRNNTYKQWGYNYFFDQSYFSKATAEN
                     SFQYGLNDKIMFADSIQYLEHLQQPFYTKFITVSNHYPYTTSLIGDEIGFPLANTDDE
                     TINGYFATANYLDASIKAFFDYLKATGLYENSVIVLYGDHYGISNSRNPDLAPLIGKD
                     SQTWSDFDNAMMQRVPYMIVVPGMTNGGIFDTYGGEIDALPTLEHLLGIKSDNYLQVG
                     QDLLSSQHSQIVALRTAGSFITPKYTSYEGKIYYTETGLEITNPDETTQKEIDAIKEA
                     TAAQLATSDAIQTGDLIRFFDNGLPALDPSQYNYIDSLAAEIALENKLGKQSTSLYST
                     NGNKSTIELFKAPSYLELHGSTTATSTPNTGTSSTSTSSNSR"
     gene            complement(7166..9670)
                     /locus_tag="AC1PNG_001186"
     CDS             complement(7166..9670)
                     /locus_tag="AC1PNG_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HIP6701694.1"
                     /translation="MKQGKTLLYVAGVSLICAVALVTYQASNYREQSNHQKTTSIETS
                     QSKQSKEIPSDSKKEVPGIDKATDDGFLLTDESQIEEKTDLGIIVNHGDHKHFFFYSD
                     LKNTKWAYLIPENYQEKNHSSQPKRSQLSSGSKQIEDEYVFDPKDIVAEDANGYTVRH
                     GDHYHYILKSSLGGTTHRQLTSDSRQSPTLPIVHQQEGIPGIDFQTSDGFLFDGQNIS
                     GVTETGILVRHGKHLHLIHFETLKRSKWSYLVNQYKPNVESDKKAQPEVENTEYQTKL
                     DYLAKELNLEPSRFKKVIVDGQIGLEYPHGDHTHIVLLKEIDTTKPFESPEDRILKQK
                     DGETLEQRKERLIKQYMERFKVKREDITIDGNYMSVRHGDHAHVYKIDPSLPDDPERD
                     VKTETVNLAIEKQLVYGPFYTEGSTENLTRNGVHQKYHPEGLQDIKNFILVTFSTNSD
                     FGDFVVNGKKTKRVYYLVRKDLNWEDLNIAYPQTLQQKGRVFNGWNATLPKSGKMARE
                     HQSFYADFDNVYRKPTKNIYTPSDDVSNIDLSDYVPVKYSAIANGRLKLNGEIRAGFI
                     YFVKSDLTWKQAKEQGLVVPEPVPSKDYEFIEFRTVITGGEKDTDYVSATTSLAAFGT
                     TAPRIGPYIAENMDNPTDINDPSRHPNYYWHDPKNYVALAFRAGDGGQLQTHLGTSKT
                     VVYLVRKGLTLNQAGIFPPSLKSDTGYKRDYTKPVIPNVAWDTPILEDTVYDIHFDKV
                     ESDSKDGTVEPGDTWLPGNPLPNTPDVPNGEEDADSWLDDLLTPSPEATDIETVTEAE
                     TTTEDTKATESSTAAPIDIPKKSSTEEEPSEDFIFP"
BASE COUNT         2859 a         2204 c         1638 g         3060 t
ORIGIN      
        1 tactccccaa gtaccgtttc tgaatctgta cttggagata agaactctac gtaagcagcg
       61 tatactaggc gtcaatccat gacctaacac cctgctccta tcaaatataa atatattaca
      121 acaattcgtt gagaggctta aagatgatac gttccaaatc agctacataa gttcccaact
      181 gttgttgctt gctgaaatat tccgtcaaaa gtggatttcc ctgaactttc ttgttgaaat
      241 ccaacatttt cttttggtca gcttcagtag gaatttcacc agcctgcaat ttagattgga
      301 tttctttttg gaaagaaata taactttcca atatttcttt ggcttctgaa ttcccctcaa
      361 ctgagacctt aacggcttcc accgccttat attcaggcag gttacggatt gcacgttcca
      421 attcatttgc gatatcatag atatttgttg acatattttt ctcctaatct aaatggattt
      481 tgtaagtcgt ctgctctctt aaacgtttcg cagcgtcttc caaatttccc ttgtttttaa
      541 acgtgatttg taaaataccg tgtatatctt cacggttctc ttcattgata cgaatattga
      601 caatggaaat accccgtaag acttccaaaa ccttcaaaat cgtatcttcc tcatcaggaa
      661 cactaaggaa taaatcgtag aaagaatcaa cacctgcacg cttgtgaatt tccatggctc
      721 tacgagtttg acgtccctta tcaaaaaagt cccaaattgc tttttgatcg ccagatctga
      781 gcacatctga aatttctgaa agacgttctt gaaattcctc gattcgattt agaatagaat
      841 ctgcattggt tagcaaaatg ctagtccaca tgctcggctc actttccgca atccgtgtca
      901 tatctctaaa tcctccagct gcaaaattat tcgtaaagga atgttcttgg gcatactcac
      961 cagcttgatg catcaaacta gaagccaata cgtgtggaaa atgtgaaatt tgactggtca
     1021 cacggtcatg ttctgctgca tcaatctgaa caaaacgtgc ccccgttcca gataacaagt
     1081 ctgtcaatcg gggaaccgca tcctgcttgg tagcctgaca cggtgtcata atgtagtaag
     1141 cattctcaaa tagatggaga tctgctgcac tggcaccaga tttatgacta cctgccatag
     1201 ggtgtccacc gataaaatta atttgtcttg gcgccaaatg ttcctcagca gcagccacaa
     1261 ttgcagactt cgttgaacct gcatctgtaa tcaagaccgt atccttcaat tcaagggcac
     1321 taaattcttc aatcaaacgc aaagaaactt gaattggaac acaaagaaga atcacatcag
     1381 cctgctttgc tacaccctcc aattccgttg atacctcatc aaccatcccc ttgcccaagg
     1441 caatttggca tgattgttcg ttaggatcaa agccaatgag gtgatagtca ggatggtctt
     1501 tgcgaataca gagcgcaagt gagcttccaa tcaaccctag acctacaatt aaaatcgttt
     1561 ttttcattaa ttacctagaa atttttgaca tagtcgcgat gactggctac tgcagctttc
     1621 aattcctcca tattatcgga agagaacttc tctaggattt ctgtcgctag agttgtagct
     1681 acgactgatt ccatgactac accagctgct gggagagccg ttggatctga acgctccaca
     1741 gtagctttat aaggctcatg agtgtcaatg tcaaccgaca tcaatggctt atagagggtc
     1801 ggaattggtt tcataacacc acgaataatc agagcctcgc cattggtcat accaccttcg
     1861 aaaccaccta gattattggt tcggcgtgta tatccttttt cttgggacca gagaatttcg
     1921 tccataactt gactcccttt gagatagcca gcttggaagc ctactccaaa ttctactccc
     1981 ttaaaagcat tgattgaaac aacagcctgc gctaattttg catctaactt tttatcccat
     2041 tgcacatatg accccaagcc agctggaact cctccaacaa cagtctcaat cactccgcca
     2101 atagtatctc catctcgctt gatttgatca atataggctt tgatttcttc ttcacgctct
     2161 tggttgacaa tggatacttc tgactgggct gcacgctctt tgatttctgc tacgcttaaa
     2221 ccatctggga catcaatttc cttccctccg aaaacgacta catggttagc aatttcaata
     2281 tccaactcag ccaaaatccg tttggcaacc gcaccaacag ccacacgcat agttgtctca
     2341 cgcgcgctag aacgctccaa ggaattacgc aaatcatcaa agcgatattt cataccacca
     2401 actaggtcag catgtcctgg tcgtggatgt ttgatacgac gcttactttt gagtttctct
     2461 tcgatgtcct caactgccat aatatccaac catttttgat ggtctttgtt ggtcacattc
     2521 aatgtaatcg gggcaccggt tgtcttaccg tgacgaacac cagctgtaat ctctacgcga
     2581 tctgtttcaa tctgcatacg actaccacga ccgtatccac cttggcgccg ttttaactct
     2641 tcattaatat cctcggctgt taaaggaaga cccgctggaa ctccctcaat aatggctgtt
     2701 agacgtggtc catgagactc accagctgtt aaataacgca taacttaatt ctccaaaatt
     2761 tccagaaact tacgagtatt gcttaaagac atttgtcctg gtgcagatgt ttcatctaag
     2821 ctggcaaaag tccagcagga gcctgtaatc acacctgcaa tacgtgttaa cttccctagt
     2881 tcacccatgg cgattgtcgc aaatatctgc tcagtgttca gtgatttaaa accacgagta
     2941 taattcataa catctaggac atcctgctct gtcttagcca ttacagccat tttaacaaca
     3001 gctggtgata ggctagtcag ttcagacatg atttccatat aattagacgg tgtttcttca
     3061 aaattatgat aactcaacac aagattagga aactccaaca tctgctcaaa aaccgactta
     3121 taagagtagt attcaaaatc aatataatct ggctgataaa gcgttgctac ttctttgatt
     3181 acattgacat attcttgatc gtccaaatcc aagtgaccgc cttcacgtgt tgtccgaatg
     3241 gtgaaaacta cttccttacc actacatttt tcaaatatag caggtgctac tcttaaaata
     3301 tcatctttcg gaagatagtc tgctcgccat tcaacaatat ctgcctctgc tagacgttct
     3361 acatcaatgg tctctacctc ttccagattt ctaggcataa taggaactac tattttcatt
     3421 tatctacctg tatcgtaaag acctttaggt aattactact ttcatcattt ttattgatta
     3481 caaaatcggc tggcaagcga taagttgcct catagcgatg ttttctaaca tcaaaccctt
     3541 tttcaatctg ccgcttaaat ttttcaacag aaacatttgc ggcgttggtc gaagcgataa
     3601 tagttccacc tgtatttagt atttccaaag attgagaaat caacttgtga taatctttgg
     3661 ctactgaaaa ggtttgtttc ttatttcgtg caaagcttgg cggatccaag acaatcacat
     3721 cataagttaa gcctttacgt ttggcatatt tgaaatattc aaatacatcc attactacaa
     3781 agtggtgatt atctaagtct aaactattaa ccttaaagtg agctttagaa agttctcgcg
     3841 agcgtttggc taagtcaaca gaagtcgttt cctctgctcc gcccatggca gcagcaatag
     3901 aaaatgctgc cgtatacgag aacatgttca aaagtgactt gcctgccgcc aggccatcca
     3961 ccaaggaacc acgtacctca tgctgatcca aaaagattcc cgtcatcagt ccatcattca
     4021 taaagacctg atagtgaact ccattttcca acacagtgaa atactcaggg gcttcttctc
     4081 cataaagatg agcagactca aaggccaaac ctttaaaacg aatcttctca taagctcctc
     4141 ttacttctgg aaatacctgt tgaaatgcag caacaatcat ctctttgaca gaataaataa
     4201 aggtattata ccatgaaaaa actgcgtatt catcatacaa gtcaaccgtc attccaccaa
     4261 aactatctcc atcttgatta aataaacgaa aagcgttcgt catctcagat tgataaaaat
     4321 gttgacgttt ctgtttagca tccacgaata atttaacaaa aaaatccatg gtcaagtctt
     4381 gattgcttct actaaacagc cagcctatcc ccttattctg tttggacaaa taagcctgac
     4441 ccaaatagct tccagactct gaatacaaaa cagccagcct gtcttcatca aatgaaacac
     4501 ctgaaaaatc agcgctatcc aagagttgaa tccctctgtt tattttctgt tctgcaatac
     4561 ggtttacctt tattttcata gaattattat aacatgtttc ttcatatatg ccaaaaaaac
     4621 tctaaacttt gaggcttatt ttagaattat ggtataatga actaaacttt tagaaaggac
     4681 catgaggttt tattgtgaaa aaactaattg actatgtcaa gaagagcgga cagactagac
     4741 ttggtttcgt cttgattttg ctaatcattt actggctcaa aacactctgg gcctactatg
     4801 ttgatttcaa cttagataca caagggtttt tccaaaattt tattgctatt atgaatcctc
     4861 taccagtcgg gattttatta ctcggtctct cgctttatat aaagaataca aaagtcttct
     4921 atagcgtagc gatgatcatt tatattattc tttccgtctg gctcttttca aatgcagtct
     4981 attacagaga attttccgat tatatttcca tctctactat aatggctacc tctagtgtat
     5041 ctgctggatt aggagaagca gctcttaagt tatttaggct atgggatatt gtctattttc
     5101 ttgattttat catttttgga atcctagtct atcgtaaact actccggtgg gataaaaaga
     5161 catttcataa aaaatctagc tttgctatta cagcactatc agtcatgctt ttctccatca
     5221 acctcttttt agcagaaatt gatagaccag agctacttac gagaggattt tctaatactt
     5281 atatcgtcag agcccttggt ttacctgctt tccttggtta caatgctaat caaacctaca
     5341 atgcccacaa gaatcgttct ggagcatcag ctacagattt ggagcctgtg aaacaatata
     5401 ttacagaaca ttatgctcag ccgaatgaca actacttcgg tattgccaaa ggacgaaatg
     5461 ttatctatct ccacctagaa agcttacaac aatttgttat tgactataag ttgaacgtca
     5521 acggtgtcga atacgaagtc acacctttcc tcaattctct gtatcattct agctctactc
     5581 tcgccttttc taatttcttc aaccaagtga aggcaggtaa aacctctgat gccgaaacca
     5641 tgattgaaac ctcacttttt ggactcaatc aaggctcttt catggtccaa tatggcggaa
     5701 gcaataccca gcaagctgca ccatatattc tctctcaaaa tggtggctat acatctgctg
     5761 tattccacgg aaatgcagcc agtttctgga ataggaacaa cacctataaa caatggggtt
     5821 acaattactt ctttgatcaa tcctatttct ctaaagccac tgctgaaaat tcattccaat
     5881 acggtctgaa tgataaaatc atgttcgccg actctatcca atatctggaa catttacagc
     5941 aacccttcta taccaaattt atcacagttt ccaaccacta cccctacaca accagcttga
     6001 ttggggacga gattggcttt ccattagcca atacagatga tgaaaccatt aatggctatt
     6061 ttgctactgc aaactatctc gatgcgtcca taaaggcatt tttcgattac ttaaaagcga
     6121 ctggcttgta cgaaaactcc gttatcgtcc tctatggtga tcactatgga atttctaatt
     6181 cacgcaatcc tgacttggca ccgttgattg gaaaagattc tcagacttgg tcagattttg
     6241 acaatgccat gatgcaacgt gttccatata tgattgttgt acctggaatg accaacggcg
     6301 ggatttttga tacctacggt ggtgagattg atgcacttcc tactctcgaa catcttttag
     6361 gaatcaaatc cgataactat cttcaagttg gtcaagacct actttccagc caacacagcc
     6421 aaattgtggc tcttcgaacc gctggcagtt tcatcacacc aaaatacact agctacgaag
     6481 gtaaaatcta ctatacagaa actggtcttg aaattacaaa tccagatgag acaactcaga
     6541 aagaaattga tgcaattaaa gaagctaccg cagcacaact agcgactagc gatgccattc
     6601 aaactggtga tcttatccga ttcttcgaca atggattgcc agccttggat ccaagccaat
     6661 ataactacat tgactcattg gctgccgaga ttgcactaga gaataaactt ggcaaacaat
     6721 caacaagttt gtatagcaca aacggcaata agtcgacaat tgaactattt aaagcaccaa
     6781 gctacctaga acttcacggt agtacaactg ctactagcac accaaatacc ggcactagct
     6841 caacaagtac cagttcaaat tcacggtaat acactaaaag gcttagaatc atgtcagtta
     6901 ctacattgat tctaagcctt tttatactca atgaaaatca aaaacagact agctccaaag
     6961 gtttggggaa cctttggagg ttggagatag agcgaacgta gttcgtttct acttagcaga
     7021 tagaaccctg ttactatttt gtttcaaggt aacaggctga aaggctccac tggagccttt
     7081 cactcatcaa atcaagtcaa caacgtctga ttttgatttt cgaagagtat tagaccacta
     7141 tttcaaatca ctcatatcta tttcatcaag gaaatataaa atcctcacta ggctcttcct
     7201 ctgttgatga ttttttaggg atatctatgg gagcagcagt actactttca gttgcttttg
     7261 tatcttctgt tgtagtttca gcttctgtta cagtttctat atctgtcgct tctggactag
     7321 gtgtcaacaa atcatctaac cagctatctg cgtcctcttc tccattaggg acatctggag
     7381 tattcggcaa tggattccca ggcaaccaag tatcacctgg ctcaactgtc ccatctttgc
     7441 tatcactttc aactttatcg aaatgaatat cgtagacagt atcttccaaa ataggtgtat
     7501 cccatgctac atttggaata acaggttttg tataatctcg tttataacct gtatcactct
     7561 tcaaagatgg cggaaaaatt ccagcttggt tcagtgttaa tccttttcta acaaggtaaa
     7621 caactgtttt actagttccc aaatgtgtct gaagttgacc gccgtcacct gctcgaaatg
     7681 caagagctac ataattcttt ggatcatgcc aatagtaatt tggatgtcta cttggatcat
     7741 tgatgtccgt tggattatcc atattttcgg caatatatgg ccctatacga ggagctgttg
     7801 tcccaaatgc tgccaagctc gttgtggcac taacataatc tgtatctttt tctccaccgg
     7861 taataactgt cctaaactcg atgaattcat aatctttgct tggaaccggt tctggtacaa
     7921 caagcccctg ctccttagct tgtttccaag ttaagtcaga tttcacaaag tatatgaacc
     7981 ctgctcgaat ctcaccattt aattttaaac gaccattggc tatcgcacta tatttaacag
     8041 gtacatagtc tgataaatca atatttgaaa catcatcact tggagtgtaa atattttttg
     8101 tcggcttacg ataaacatta tcaaaatcag cataaaaact ctgatgttct cttgccatct
     8161 tgccagactt tggcaaagta gcattccaac cattaaagac gcgtcccttt tgttgaaggg
     8221 tctgcggata ggcgatgttc aaatcctccc aatttaaatc tttacgaacc aaataataaa
     8281 ctctcttcgt ttttttacca ttaacgacaa aatcaccaaa gtcactattt gtactgaatg
     8341 ttactagaat gaaattttta atatcctgaa gaccttccgg atgatacttt tgatggacac
     8401 cattacgtgt taaattttct gttgaacctt ctgtataaaa aggtccatat actaattgtt
     8461 tctcaatagc aagatttacc gtttcggtct taacatcacg ctcaggatca tctggcaaac
     8521 taggatcaat tttataaacg tgcgcatggt ctccatggcg tacactcata tagttcccat
     8581 caattgtaat gtcctcacgt tttactttaa aacgctccat gtactgttta atgagacgtt
     8641 cttttctctg ttcaagagtt tctccatcct tttgtttaag aatacgatct tccggacttt
     8701 caaacggctt cgttgtatca atttccttta ataaaacaat atgtgtgtga tctccatgcg
     8761 gatactctaa tccaatttga ccgtcaacga tgactttctt aaaacgtgat ggctctaaat
     8821 tcagttcttt tgctaagtag tccaatttgg tttgatactc agtattttct acctcaggtt
     8881 gtgccttctt atccgattca acatttggtt tatactgatt cacaagataa gaccatttac
     8941 tccttttcaa tgtttcaaaa tgaataagat gcaaatgttt cccatgcctt actagaatcc
     9001 cggtttccgt tacaccgctg atattttgcc catcaaacaa gaaaccgtca ctcgtttgaa
     9061 aatcaatccc cggaatccct tcctgttggt gaacgatggg aagagttggc gattgacgac
     9121 tatcagaggt caattgtctg tgtgtcgtgc cacctaagct tgattttaaa atatagtgat
     9181 aatgatcccc atgccgaaca gtatagccat tggcatcctc tgcgacaata tctttaggat
     9241 caaaaacgta ttcatcttcg atctgtttcg agcctgacga taattgactt ctcttaggtt
     9301 gagaagaatg atttttttct tgatagtttt caggaattaa ataagcccat tttgtattct
     9361 ttaaatctga ataaaagaaa aagtgcttat gatcgccatg attgacaata attcctaaat
     9421 cagttttttc ctcaatttgt gactcatcag tgagtaaaaa accatcatcc gtcgccttat
     9481 caatccctgg aacttccttt ttcgaatctg aaggaatctc ttttgattgc tttgattgcg
     9541 atgtttctat gcttgttgtt ttttgatggt tcgattgctc tctatagtta gacgcttgat
     9601 aagtaaccaa tgctacagca caaataagcg atactcctgc tacataaagc aaagtcttac
     9661 cttgtttcat atctatgttt ctctccttat ttaccattat tatattaccg acacgataaa
     9721 tgtaatcaac tattgtaaaa tagcatcacc acatccactt a
//
LOCUS       DBNFEG010000073         9690 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.073, whole genome shotgun sequence.
ACCESSION   DBNFEG010000073 DBNFEG010000000
VERSION     DBNFEG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.073"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            26..808
                     /locus_tag="AC1PNG_001187"
     CDS             26..808
                     /locus_tag="AC1PNG_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIP6701695.1"
                     /translation="MRKWKKIIIGCFAILTVCLLFAPTLARANQIPDRPTGTTVVDET
                     QLLSSETIAEIDQLNRSWASTEQALQVGVYVTESLSSDIESLANETFRHWQVGFAGTD
                     NGVLLVVAIADRAFRIETSDNAATVITDVEAKDILENAREFFRQEDYSGGISYIVNSI
                     GDRFYGTSIGKNQLAAIEEGTSEKDDGFWLFLIVILIAILFGIIDKSSRGGGGPGNLL
                     WMLVDDHHHYHNHHSSNSSSSSFGGGSWSGGGGGGGGASSGW"
     gene            881..1462
                     /locus_tag="AC1PNG_001188"
     CDS             881..1462
                     /locus_tag="AC1PNG_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIP6701696.1"
                     /translation="MKKKWLAILLPILALLFLGMGAVGQYNGLVDSYAEVENAQANVD
                     TQLQRRYDLIPNVVAAVKGAMEHEEEIFTAIAEARAKIGSSQVGTSDYNQAQSQLDSA
                     VSRLLVVAENYPQLTANQQVSALITELEGTENRILVARKDYNSVATTYNKKIKRFPTS
                     IYANLFGYEKVELFQATNDAATTVPSVDLSDKE"
     gene            1474..2937
                     /locus_tag="AC1PNG_001189"
     CDS             1474..2937
                     /locus_tag="AC1PNG_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0016600 - flotillin complex [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HIP6701697.1"
                     /translation="MDFGFDILAIMIPIIVVVLFVLLFVFGYVSAKPNEAIVITGLGK
                     PRTLIGRSGFMIPFIEKRSYISIEQFSTDVQTTDFVPTLDFINVKADAVVKVKVGISD
                     ELLNAAAQNFLNWKTADISASIQDVLEGNLREIIGQMELRDMVNNRQAFAEKVQSNAA
                     PDLAKMGLEIIAFTVQSFTDDNDVIKNLGIDNIVTIQKDAANARAKAEREQAEVRARE
                     DKAANDARVAADLEIAKKQNELAIEQANLKRQSDVQLAQANAAYGIEEQAQRKEIERA
                     TAEANIVKQQKEAEVKAEEVKVREQELSATIRKQAEAEKYARQQAAEADLIERQRKAE
                     AELYETQREAEAQKARAEAAKYAAEQEAAGIEAKGRAEAEAIRLKLEAEAEGLSKKAD
                     AMAKFNDAAVTEMVVNVLPEIAKNIAAPLGNVDKITMYGEGNASKMVGDLMTTMDKTT
                     EGLGLNVRDLISATLTGRAMSTGLLTAQETKEAEGDK"
     gene            3026..3334
                     /locus_tag="AC1PNG_001190"
     CDS             3026..3334
                     /locus_tag="AC1PNG_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701698.1"
                     /translation="MKMFRNSKKSKLFIQKINELLSDSELKLSKALKFQLLEAMELCE
                     KGSKISYLSYKIYPWVLEELALNRIQSDKLKMFKRYLEQERWKYYFGSALGMAFTSIR
                     "
     gene            complement(3456..4531)
                     /locus_tag="AC1PNG_001191"
                     /pseudo
     CDS             complement(3456..4531)
                     /locus_tag="AC1PNG_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647074.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4643..6016)
                     /gene="mnmE"
                     /locus_tag="AC1PNG_001192"
     CDS             complement(4643..6016)
                     /gene="mnmE"
                     /locus_tag="AC1PNG_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIP6701699.1"
                     /translation="MITKEFDTITAISTPLGEGAIGIVRLSGTDAFAIASKVFKGKDL
                     ATVPSHSLNYGHIIDPATGQVLDEVILGVMRSPKTFTREDVIEINTHGGIAVTNEILQ
                     LLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDVIRAKTDKAMHNAVRQLDGSLSQ
                     LINDTRQEILNTLAQVEVNIDYPEYDDVEEATTELVREKTLQFQALLENLLRTARRGK
                     ILREGIATAIIGRPNVGKSSLLNNLLREEKAIVTDIAGTTRDVIEEYVNIKGVPLKLI
                     DTAGIRETDDIVEKIGVERSKKALEEADLILLVLNASEPLTEQDRNLLAISDLANRIV
                     LLNKTDLVEQIEADQLPEDVIRISVLKNQNIDQIEEKINQLFFENAGLVEQDATYLSN
                     SRHISLIEQAVQSLQAVNDGLEMGMPVDLLQVDLTRCWQILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            6700..>9690
                     /gene="cas9"
                     /locus_tag="AC1PNG_001193"
                     /gene_synonym="csn1"
     CDS             6700..>9690
                     /gene="cas9"
                     /locus_tag="AC1PNG_001193"
                     /gene_synonym="csn1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962169.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HIP6701700.1"
                     /translation="MSNGKILGLDIGIASVGVGVIDAQTGEIIHASSRIFPSANAANN
                     AERRTFRGSRRLIRRKKHRIKRLDDLFNDFHINLDGEMSTDNPYVLRVKGLSQKLTVE
                     ELYISIKNIMKRRGISYLDDAESDNEAGRSDYAKAIERNRQLLTSKTPGEIQLERLEK
                     YGQLRGNFTIIDEEGQSQQIINVFSTSDYVKEVEKILDCQKMYHKFISDEFCDKLIEL
                     LREKRKYYVGPGNEKSRTDYGIYRTDGTTLENLFGILIGKCTFYPDQYRSSRASYTAQ
                     EFNFLNDLNNLTVPTETKKLSQEQKEFLVNYAKETSVLGAGKILQQIAKLADCKVEDI
                     RGYRLDNKDKPELHTFETYRAMKGLVPLVDIGVLSREQLDILADILTLNTDFEGIREA
                     LKKQLPNVFDEKQVKGLASFRKSKSQLFAKGWHNLSQKIMLEVIPELYATSDEQMTIL
                     TRLGKFEKSSVAEYPSSINVDEITDEIYNPVVAKSIRQTIKIINASIKKWGEFDQIVI
                     EMPRDRNEDEEKKRIADGQKANAKEKADSILRAAELYCAGKVLPDYVYNGHNQLATKI
                     RLWYQQGERCIYTGQPISIHDLIHNQNQYEIDHILPLSLTFDDSLSNKVLVLATANQE
                     KAQRTPYNYLKSATSAWSYREFKDYVTKRKGIGKKKCEYLTFEEDINGFEVRSKFIQR
                     NLVDTRYASKVILNALQDYFKISGIQTKVSVVRGQFTSQLRHKWGIEKTRETYHHHAV
                     DALIIAASSQLRLWKKQESPLVVDYQEGRQVDLETGEILELTDEQYKELVYQPPYQGF
                     VNTISSSAFDNEILFSYQVDSKVNRKISDATIYATRNAQLGKDKTEGIYVLGKIKDIY
                     TQAGYEAFLKRYTKDKTSFLMYHKDLDTWEKVIEIILRDYREYDEKGKEIGNPFERYR
                     RENGYVKKYSRKGNGTAIKSLKYYDNKLGNHIDITPENSRNAVVLQSLKPWRTDVYFN
                     KETGKYEFLGIKYSDLSFEKGTGEY"
BASE COUNT         3002 a         1694 c         2262 g         2732 t
ORIGIN      
        1 tttgttcatc gaaggaggtg ttctgatgag aaaatggaaa aaaattatca tcggctgttt
       61 cgctattcta acggtttgct tgctatttgc acccacacta gctagagcaa atcagatccc
      121 agaccgaccc acaggtacaa cggtagtgga tgagacgcag ttactctcga gtgagaccat
      181 tgcagaaatt gatcaactca atcgaagttg ggcaagcacc gagcaagctt tacaggttgg
      241 tgtttatgta acggaaagtc tgtctagtga tattgaaagt ttggctaatg aaacctttcg
      301 tcactggcag gtcggttttg caggaacgga caacggagtt cttttagtgg ttgccatagc
      361 tgacagagca tttcggatag aaacctcaga caatgctgcc acagtcatca cggatgtaga
      421 agccaaggat attctagaga atgcaaggga attctttcga caggaagact acagtggagg
      481 aatttcctat atcgtcaact ccattgggga taggttttat ggtacaagca ttggtaaaaa
      541 tcagttagct gctatagaag aaggtacaag tgagaaggat gacggcttct ggctgtttct
      601 catagttatt ctgatcgcta tcctatttgg tattattgac aaatctagtc gtggtggtgg
      661 aggtccaggc aatctactct ggatgttggt tgatgatcat catcactatc acaatcatca
      721 ctcatctaat tcctcatcgt ctagttttgg cggaggtagt tggtcaggtg gtggaggcgg
      781 cggtggcgga gcctcttctg gatggtaagg catcgtataa ggcttctgat agttgtttgc
      841 ttttttacga atgtttaatc gtagttagaa aggaaatctt atgaaaaaga aatggttagc
      901 catacttctc ccgattctcg ctctgctgtt tttaggaatg ggagcagttg ggcaatacaa
      961 tggcttagtt gacagttatg ctgaggttga aaatgcgcaa gccaatgtag acacacagtt
     1021 gcaacgtcgc tatgacttga taccaaatgt agtcgcagct gtcaaaggcg ccatggaaca
     1081 cgaggaagaa atatttactg ctattgcaga agcgcgtgct aaaatcggtt ccagccaagt
     1141 aggaaccagc gactacaatc aggcacaaag tcagctagat tcggcagtgt ctagattgtt
     1201 ggtagtggct gagaattatc cgcaattaac agccaaccaa caggtatctg ccctgataac
     1261 agagcttgaa ggtacggaaa atcggatact ggtagcccgt aaagactata attcggtagc
     1321 aactacctac aataaaaaaa tcaagcggtt cccgacctcc atctatgcta acttatttgg
     1381 ctatgaaaaa gtcgagcttt tccaggcaac aaatgatgcg gcgacgactg taccaagtgt
     1441 tgatttgagt gataaggaat aaaggagata aatatggact ttggatttga tatattggca
     1501 attatgatac caattattgt tgttgtacta tttgttttat tgtttgtatt tggttatgtg
     1561 tctgctaagc ctaacgaagc gattgtcatt actggcttgg gcaaacctcg gactttaatt
     1621 ggtcgttctg gttttatgat tccctttatc gaaaaacgct cctatatctc tattgagcag
     1681 ttttcaacag atgtgcagac cacagatttt gtaccgactc ttgattttat taacgtaaaa
     1741 gctgacgcgg tcgtaaaagt aaaggttggg atatccgatg aattgctcaa tgcggcagct
     1801 caaaacttcc ttaactggaa aactgcagat atttctgcct ctattcaaga tgtcttggaa
     1861 ggaaatctgc gtgaaatcat tggccagatg gaactgcgtg acatggttaa taaccgccag
     1921 gcttttgcag aaaaagtcca atccaacgca gctcctgact tggctaaaat ggggctggaa
     1981 atcattgcct ttacggttca atccttcaca gatgacaacg atgtcattaa aaacctgggt
     2041 attgacaata ttgtgaccat ccagaaggat gccgcaaatg ctcgtgccaa agcagagcgg
     2101 gaacaagcag aagtccgtgc ccgtgaggat aaggcggcta atgatgcgcg tgttgctgca
     2161 gacttagaaa tcgccaagaa acagaatgaa ctagctattg agcaagctaa tctaaaacgt
     2221 cagtccgatg tccaactggc acaggcaaac gcagcctatg gcattgagga gcaagctcaa
     2281 cggaaagaaa tcgagcgtgc tactgctgaa gccaatattg tcaagcaaca aaaagaagca
     2341 gaagtaaaag cagaggaagt taaggtccgt gaacaagagc tctcagctac tatccgcaaa
     2401 caggcagagg cagaaaaata cgctcgtcaa caagctgcag aagcagattt gattgaacgt
     2461 caacgcaagg cagaagcaga actctacgaa acgcagcgtg aagcggaagc tcaaaaagcg
     2521 cgtgccgaag ctgctaagta tgctgcggag caagaagcgg caggtattga ggccaaagga
     2581 cgtgccgaag cagaagccat ccgtttgaaa ctcgaagcag aggcagaagg tcttagcaag
     2641 aaggcggatg ccatggctaa gtttaatgat gcagcggtga cagaaatggt ggttaatgtt
     2701 cttccagaaa tcgccaagaa tattgctgca ccacttggaa atgttgataa gattaccatg
     2761 tacggcgaag ggaatgcctc aaaaatggtg ggagacctga tgacgaccat ggacaagaca
     2821 acagaaggtc taggactcaa tgtccgtgat ttaatcagcg caaccctaac tggtcgtgcc
     2881 atgtcaacag gtttactgac tgcccaagaa acaaaggaag cggaagggga caaataaaaa
     2941 taataagcag ttggaagaga tgggtgactg agtaggttta caccatcatt tccatcgcat
     3001 gattgtttca gaaatcccta cgaagatgaa aatgtttaga aacagtaaaa aaagtaagtt
     3061 gtttatacaa aaaataaatg aactattatc tgattcagaa ttaaagttga gcaaagcatt
     3121 gaaattccag ttgttggaag caatggagtt gtgtgaaaaa ggtagtaaga ttagctatct
     3181 ttcatataaa atttatccat gggtattgga agaactagct ttgaatagga ttcaatcgga
     3241 taaattaaag atgtttaagc gctatttaga gcaagaaagg tggaaatatt attttggttc
     3301 tgctttggga atggcgttta cttcgattag ataatggttc cgaggatttg taagtacatt
     3361 gattgtgctg tgcaacttcg atagaatatg cgaaagtccc aagaaagagt aatcttggga
     3421 cttttacaat ctttattgag attcgttttt gtttatcagt catttaccca cactccgctg
     3481 acataattct cagatagaat ggtttgactt aaaacttttt ttgcttgcca gtcatagatg
     3541 agtgccttac cagccagaat caataataat ctgttaccgt ctagttgacg gacatggtca
     3601 atataataag gacgaggatt aagctcgtct agacgatggt agctctcttg acctgtttgg
     3661 gtattgtaaa tggtaaaaga gattgggcta aacatgtcat tttgatgttc gatgaggaga
     3721 agcttctggt ctttgagagc atgtagtgtg cttggtgagg ggtgttctaa agttactgac
     3781 gaaaatgttt ttgtttttgt atcaaaggtt aggagttcgg atgactgttc aatgtcagta
     3841 tactcacttg ttttttccct aaatttggga gatagatggt tccattgacc caaggcccct
     3901 ctcctgtggc aatggtattg tcgtacttga atacctcttg ttttgaaaaa tctttcttat
     3961 caaatacaat caagacattt tcttcgggtc cttcttgggt aaaatctttc acaaaaccag
     4021 ttaaatagac tgacgtttgg tccaccatga ctggagcatt tgggatgaag tcatcgttta
     4081 aaacgtgggt ttgaatggta tcaaaattcc tgtcaaattg cgctagtatg tgatagggcc
     4141 ccgctgtata aaagtcttgt ccatctgtac cactgatata gctactatac tgacttggct
     4201 ttcgcaagac agtgccagta ttgaaatcga tggatatcag attgtcgtta atgaattttt
     4261 gctgatcgtt ggcaaagact agataccgac tgtctatgac ataattttca ccagaaccaa
     4321 aatacccttc tgaattcatt ttttgggtat gaagcagctc tggttcttga ccatttagct
     4381 gaaaggcttc gaccttaccg ggataaatca agtaaaaatc agcttccgac gttaatgtga
     4441 ttgtattgtg ctggtaggca tatagtttcc agccgataaa tggaaagaca agacaggata
     4501 gaaggacaat acaaataaga agttttttca tgggaatgcc gcctttcact gtttagtata
     4561 tctaaatttg tcatcttaga caagaaatta tgtgttaata tacaaataaa gtcatgcaag
     4621 ttttcttaca tgacttttgt tcttattttc caagacagaa ttggctaaag agttgggtaa
     4681 tgagttcatc tggagcagca tcacctgtta tttctcccaa aatttgccaa cagcgtgtca
     4741 ggtcaacctg taacaagtct actggcatgc ccatttccag cccatcgttg acagcttgta
     4801 ggctttggac tgcctgttca atgagggaaa tgtggcgaga attggagagg taggtggcat
     4861 cttgctcgac cagacctgca ttttcaaaga agagttgatt gattttttct tcgatttggt
     4921 cgatattctg gtttttcaag acggaaatgc ggatgacatc ctcaggcagt tggtctgctt
     4981 caatctgttc taccaggtcg gtcttgttga gtaggacgat acggttggcc aggtctgaaa
     5041 tggctaggag gttgcgatct tgctcggtca ggggttcaga tgcgttgagg actagcagga
     5101 tgaggtcggc ctcctcaagg gcttttttag agcgctccac accgattttt tcaacgatgt
     5161 cgtctgtttc acggatacct gctgtatcaa tgagcttgag ggggacgcct ttgatgttga
     5221 cgtattcttc gataacgtcg cgggttgttc ctgcgatgtc tgtaacgatg gctttttcct
     5281 cgcggaggag gttattgagc aggctggatt ttcccacatt tggacgaccg ataatagcgg
     5341 ttgcgatgcc ttcacgcagg atttttccac ggcgggcagt acggaggaga ttttctagaa
     5401 gagcttggaa ctggagagtt ttctcgcgga ccagctctgt cgtcgcctcc tcgacgtcgt
     5461 catactcagg gtagtcgatg ttgacctcga cctgtgccag cgtgttgaga atctcctggc
     5521 gggtgtcgtt aatgagctgg gagagggagc cgtcaagctg gcggacggca ttgtgcatgg
     5581 ccttgtcggt cttggcacgg ataacatcca tgacggcctc ggcctgtgtc aaatccacac
     5641 gaccgttgag aaaggctcgc ttggtaaact cgcccggctc agccattcga gcaccttggc
     5701 gaatcaggag ctggagaatt tcgttggtaa cggcgatgcc tccgtgggta ttaatctcaa
     5761 tgacatcttc gcgcgtgaag gtctttgggg aacgcatgac acccagtatg acctcgtcaa
     5821 gcacttgccc tgtcgcaggg tcaataatgt ggccgtagtt gaggctgtgg ctaggaaccg
     5881 ttgctaagtc ctttcccttg aaaaccttgc tggcaatggc aaaagcatcc gtcccagaca
     5941 ggcggacaat cccgatagcg ccctcaccga gaggggtgga aatggctgtt attgtgtcga
     6001 attctttggt gatcataata atgtgctttc taactagaat ctgtatttac aagtaaaata
     6061 cttggatata agagatagtt tgttgctaat caaggcagtt ttttggggga tgggctcaag
     6121 cccagtgcca cttctcagag ttcgtgtcaa catctcagcg cagtggttga ttggctttaa
     6181 cagtccagtg gactgttaaa ggttggagat aggatttgtg aagcaaatct ctgcaattcg
     6241 tgtttcacac tccaaatccg acctctacga ctgttgcgaa caagttcgct tcatttccaa
     6301 cctccaacag tcactactct gactgttgga gctgtgcggg ggtgggagta aaatagtccg
     6361 gtggagtgaa acagtttagg gaactgtttc agctggtcac cttaaaacta gaaagtgacc
     6421 atagaagctg aacctactgt attggaagtg agggaaactc gttttgagat caatcgagtt
     6481 ctttctcact ccccaaaaac taacgatgaa tagctgataa actagcttta gatggaagtg
     6541 ctgaactgtg aatacaggtt cttaaatctt acttataagt ttaacatgga atgaagaaaa
     6601 aagcacattt tagagatgct gatacaaaga tatgtattga aaacgttttg aaaaaggtgt
     6661 aaaatagatg tggaataata aagagaaagt aggttttgta tgtcaaacgg taaaatttta
     6721 ggccttgata ttggtattgc ttccgtcgga gtaggcgtca tagatgcaca aacgggagaa
     6781 atcattcatg caagctctcg tatttttccc tctgcaaatg ctgctaataa tgcagaacgc
     6841 cgaacttttc gtggaagtcg tcgattgatt aggagaaaaa aacatagaat taaaagatta
     6901 gatgatttat ttaatgattt tcatattaat ttagatggag aaatgtcgac agataatcca
     6961 tatgttcttc gcgtgaaagg cctctctcaa aaactaacag ttgaagaatt atatatttct
     7021 ataaagaata ttatgaagcg cagggggata tcctatctag atgatgcaga gtcagataac
     7081 gaggcgggaa gaagtgatta tgctaaagct atcgagcgta atagacagtt attgactagt
     7141 aaaacaccag gggaaattca gttagagcgc ttagagaaat acggtcagtt gcgtggaaat
     7201 ttcacgataa ttgatgagga ggggcaatct caacaaatta tcaatgtttt ttcaacatca
     7261 gattatgtca aggaagttga gaaaatttta gattgtcaaa aaatgtatca taaatttata
     7321 tctgatgaat tttgcgataa gctgatcgaa ctattaagag aaaaaagaaa gtattatgtt
     7381 ggtccaggga atgagaaatc tagaactgac tatggtattt atagaacaga tgggacaact
     7441 ctagagaatt tatttggaat tcttattggt aaatgtacat tttatccaga tcaatatcgt
     7501 tcttcgagag cgtcctatac agcacaagaa tttaattttt taaatgattt aaacaattta
     7561 acagtaccta ctgaaacgaa aaagttaagt caggaacaaa aagaattttt agtaaattat
     7621 gctaaagaaa cgtcggtatt aggggctgga aaaatattgc aacaaattgc gaaacttgcg
     7681 gattgtaagg ttgaagatat tagggggtat cgtttagata ataaggataa acccgaacta
     7741 catacctttg aaacatatcg agcaatgaaa ggtctagttc cgttagtaga cattggagta
     7801 ttgtcgagag agcagcttga tatattagcc gatattctaa cccttaatac tgattttgaa
     7861 ggaattagag aggccctaaa aaaacaattg ccaaatgtat ttgatgaaaa acaggtcaag
     7921 gggttagcaa gttttagaaa gtcgaaaagc caattatttg caaaaggctg gcacaatcta
     7981 tctcaaaaaa ttatgctaga ggtaattcca gaattatatg caacatctga tgaacaaatg
     8041 acaatcttaa cacgattagg aaagtttgag aagagtagtg ttgctgaata tccttcctca
     8101 attaatgtag atgagattac agatgaaata tataatccag tcgttgctaa atctatccgt
     8161 caaaccatta aaattatcaa tgcttcgata aaaaaatggg gtgaatttga tcaaattgtt
     8221 atagagatgc ctcgtgatcg gaatgaagat gaagagaaga agcgaattgc ggatggacag
     8281 aaagcaaatg caaaagaaaa agcggattca atcctacgtg cggcagaatt gtattgtgct
     8341 ggaaaggtat tgccagatta tgtctataat ggacacaatc agttagcaac aaaaattcgt
     8401 ctctggtatc aacaaggaga gcgttgtatt tatactggtc agccgatctc aattcatgat
     8461 ttaattcata atcagaatca gtatgaaatt gaccatatct tgccactttc tttgacattt
     8521 gatgatagtt tatcgaataa ggttttagtt cttgcaactg caaaccaaga gaaagctcaa
     8581 cgtacaccgt ataattatct gaaaagtgct acgtcagctt ggtcctatcg tgaatttaag
     8641 gactatgtta ctaagcgcaa aggaattggg aagaagaaat gtgaatatct gacatttgag
     8701 gaagatatta atggatttga ggtgagaagt aagttcattc agcgtaactt agtggatact
     8761 cgatacgcct ctaaagtcat tctaaatgcc ttacaagatt atttcaaaat atcagggata
     8821 caaacgaaag tttctgttgt aagaggtcaa tttacatctc aattgcgtca taaatgggga
     8881 attgaaaaga cacgtgaaac gtatcatcac catgcggtgg atgccttaat tattgcagca
     8941 tcaagtcaat taagactctg gaaaaagcaa gaaagcccgc ttgttgttga ttaccaggaa
     9001 ggacgtcaag tagacttaga aactggagaa atcttagaat tgacagatga gcaatataaa
     9061 gaacttgtct atcaaccgcc gtatcaagga tttgtaaata ctatctctag ctcggctttt
     9121 gataatgaga tactgttttc ttaccaagta gactccaagg tgaatcgtaa aatttctgat
     9181 gcaaccattt atgcaactcg taacgcacaa cttggaaaag ataaaacgga agggatatat
     9241 gtccttggaa aaatcaaaga tatctataca caagctggat atgaggcatt tttgaaacgt
     9301 tatacgaagg acaaaacttc ttttctaatg tatcataaag accttgatac ttgggaaaaa
     9361 gtgattgaga ttattttacg tgactataga gaatatgatg aaaaagggaa agaaataggt
     9421 aacccatttg aaagatatcg tagggaaaat ggttatgtga aaaaatatag ccgtaaagga
     9481 aatggtactg caataaaatc attaaaatac tatgataata agcttggaaa tcatatcgat
     9541 attactccag aaaatagtcg aaatgcggta gtattgcagt cattaaaacc ttggaggaca
     9601 gatgtatatt ttaataaaga aacaggcaag tatgaattct tggggattaa atattctgat
     9661 ctatcgttcg aaaaaggtac tggagagtat
//
LOCUS       DBNFEG010000074         9567 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.074, whole genome shotgun sequence.
ACCESSION   DBNFEG010000074 DBNFEG010000000
VERSION     DBNFEG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9567
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.074"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..652
                     /locus_tag="AC1PNG_001194"
     CDS             <1..652
                     /locus_tag="AC1PNG_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIP6701701.1"
                     /translation="RFFGKIYGVVAMGIGLSALVSFLAVTVFQSLLLSLLSAGSIIMM
                     LIMIGQIALVVSASAMAAKNSPMALPMFLAYSVTNGITISMILMFYTSETVVLAFVSA
                     ALMFAIMAVIGMTTKKDLSGMAQALRAALWGIIIASVINIFLRSSGLSFMMSIISVLV
                     FSGLIAYDNQRIRNVFEQTGGNVGQGWVVSMALQLYLDFINLFLNLLRIFGGLSRD"
     gene            complement(702..2033)
                     /gene="pepC"
                     /locus_tag="AC1PNG_001195"
     CDS             complement(702..2033)
                     /gene="pepC"
                     /locus_tag="AC1PNG_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HIP6701702.1"
                     /translation="MSTLDFDFTERLYANYLANPSLQATENAVSHNGLLKSLETRQSA
                     IDNDYVFSIDLTKDAVSNQKASGRCWMFAALNTFRHKLISDFKLENFELSQAHTFFWD
                     KYEKSNWFLEQIIATADQEIGSRKVKFLLDTPQQDGGQWDMVVALFEKYGVVPKSVYP
                     ESISSSASRELNQYLNKLLRQDAQILRDLLAKGASSKEVQIQKENLLQEIFNFLAVNL
                     GLPPRSFDFAYRDKDNVYHRNTNVTPQAFYEKYVGLKLSDYVSIINAPTTDKPYNKSY
                     TVELLGNVVGAPAVRYLNVEMNRFKELAIAQLKAGESVWFGSDVGQSSNRQTGIMATN
                     TYDFSSGLGIHFHQDKAGRLDYSESLMTHAMVLTGVDLDDNEQPLKWKVENSWGDKVG
                     DKGYFVASDSWMDEYTYQIVVRKEFLTQEELAAYQAQPQVLAPWDPMGALA"
     gene            2167..3414
                     /locus_tag="AC1PNG_001196"
     CDS             2167..3414
                     /locus_tag="AC1PNG_001196"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIP6701703.1"
                     /translation="MTKTPFVSKEVLETITEQFPTPFHLYDEKGIREKARALNAAFAW
                     NKGFKEYFAVKATPTPAILKILQEEGCGVDCATDVEVLMSEKLGFKDIMFTSNDTQAQ
                     EFVYARKVGATINLDAYEHIEFLKNVAGIPETVCLRYNPGGVFSLGTDIMDHPEESKF
                     GMTKEQLMKGYKELKELGVKEFGIHAFLASNTVTNDYYPVLAHQLFELALEIREETDV
                     TLDFIDLSGGIGVNYRPEQEPNDIAVIGEGVRKVYEEILTPAGMGHIKIFTELGRFML
                     APHGHLITKVLHRKETYRTYIGVDASAANLMRPAFYGAYHHITNITRPDAPIEVVDVA
                     GSLCENNDKFAVNRELPRAEVGDTLVIHDSGAHGFSMGYNYNGRLRSSEILLQEDGTA
                     RMIRRAETPEDYFATIYGFDFDR"
     gene            3517..3753
                     /locus_tag="AC1PNG_001197"
     CDS             3517..3753
                     /locus_tag="AC1PNG_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HIP6701704.1"
                     /translation="MNLGLAILLIVLAFAGGVALGIYLSRKQVENYIADKPVLDENAL
                     RLMMSQMGQKPSEAKVQQVLRQIKSQQKVASKKK"
     gene            3843..4637
                     /gene="racE"
                     /locus_tag="AC1PNG_001198"
     CDS             3843..4637
                     /gene="racE"
                     /locus_tag="AC1PNG_001198"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIP6701705.1"
                     /translation="MDNRPIGFLDSGVGGLTVARELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKNVKMIVFACNTATAVAWEEVKEKLDIPVLGVILPGASA
                     AIKATQTGKVGVLGTAMTIQSDIYRQKIQALSPETQVDSLACPKFAPLVESNSHQSSL
                     AKKVVYETLRPLVGKVDTLVLGCTHYPLLRPIIQNAMGKDVKLIDSGAECARDISVLL
                     NYFQINRSRTEKDIQHRFYTTASPAAFKEIAESWMGIDIHVEHVEL"
     gene            4634..5644
                     /locus_tag="AC1PNG_001199"
     CDS             4634..5644
                     /locus_tag="AC1PNG_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HIP6701706.1"
                     /translation="MTDKIYEYRDEHNWFIGKASFANLFGSFGENGREQEIYQIGQLF
                     DKLIAGNYEDENFNQCVNIEVIKLQSEFALFQFACDTLNELNNRQFKVLQHQGAILVT
                     ENDKLLLVHLPQAGVSTADFFGQDKGLASVGDTILIATKNEGKTKEFRKFFERFGYQV
                     ENLNNYPDLPDVAETGMTFEENARLKAETIAELTGKMVLADDSGLKVDALGGLPGVWS
                     ARFSGPDATDELNNAKLLHELAMVFELKDRSAQFHCTLVMAAPNRDSLVVEADWEGFI
                     GMDLRGEDGFGYDPLFLVGETGKTSAELTLEEKNKISHRAQALEKLVEAFPVWQEQAK
                     QS"
     gene            5617..6144
                     /locus_tag="AC1PNG_001200"
     CDS             5617..6144
                     /locus_tag="AC1PNG_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIP6701707.1"
                     /translation="MAGASKTILVMSDSHGDRQIVEDIKHHYLGKVDAIFHNGDSELD
                     SQDSLWDGIQVVNGNCDYFGGYPDQLITQLGDVTIAQTHGHLYGINYGWQRLDYWAQE
                     VDADICLYGHLHVPDAEVRGKTLFLNPGSVSQPRGLVRECLYALVTIYDDHFHVDYYN
                     RQHQLYPALTKDISR"
     gene            6141..6599
                     /gene="cbpB"
                     /locus_tag="AC1PNG_001201"
     CDS             6141..6599
                     /gene="cbpB"
                     /locus_tag="AC1PNG_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HIP6701708.1"
                     /translation="MIAREFEVFLLDQEGTFLTPADKLAVIIDTHNIDHAKLLLSHMT
                     YSRVPVVTEDGRFFGTIGLTEIIKYQAENELSDDELNRDISVIVKTDEETVGLDYDLT
                     EVMRKLIDQSFLPVLGENREFLGIITRKSILKAINALLHNFPLASKEGKK"
     gene            6596..7327
                     /gene="xerD"
                     /locus_tag="AC1PNG_001202"
     CDS             6596..7327
                     /gene="xerD"
                     /locus_tag="AC1PNG_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIP6701709.1"
                     /translation="MKQAIESFIQSKKVSVNSQKSYTYDLQQFVTVTKGEISQQSLLV
                     YQQSLLDLKPAAQKRKMSAVNQFLYFLYENNLLDRFYKLQTMSEPASVKKKLEREDLT
                     LLFQESPWLDGQLITLLIALLGLTPSEIAELTSQQVNLDFQVLTVEKGGAKRVLTLPK
                     ELIPYMESHLSGRYVFDKKGQTYSRQWFFNRLTEFVQSIGKPDWTAQKLREQYILKHM
                     DEGKSLDQIAKQLGLKTSMSLEKFR"
     gene            7327..8046
                     /locus_tag="AC1PNG_001203"
     CDS             7327..8046
                     /locus_tag="AC1PNG_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HIP6701710.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQVDIYEVPITEVIEQYLAYIAT
                     LQAMRLEVAGEYMLMASQLMVIKSRRLLPKVVEQIDPEDDPEMDLLDQLEEYRKFKLL
                     SEKLGEQHDERANYFSKPKLDLIYDDVQLAKDKTIIDIFLAFSKVMAEKQASLRQSHA
                     TIARDEYKIEDMMDFVRSRFETGPRLELRQLFQESQDVNEMITIFLATLELVKVHEIV
                     LEQTETFGDIYLVRSEDESLS"
     gene            8030..8599
                     /gene="scpB"
                     /locus_tag="AC1PNG_001204"
     CDS             8030..8599
                     /gene="scpB"
                     /locus_tag="AC1PNG_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIP6701711.1"
                     /translation="MNRLAEIEVLLFVAGEDGLTLRNLAEMLEMQPTAVSQQLEKLSE
                     KYMADKASGLAILESSNRYKLVTKKEYANLLRIYAKTPINQTMSRALLETLSIVAYKQ
                     PITRIEVDDIRGVNSSGAISKLQAFDLIRENGKKEVLGRPNLYVTTDYFLDYMGINSL
                     EELPDVSEIDLIQEETELFVERNELENEN"
     gene            8589..9320
                     /locus_tag="AC1PNG_001205"
     CDS             8589..9320
                     /locus_tag="AC1PNG_001205"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIP6701712.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELGTVIKSG
                     DKVEVEGKPIYNEEKVYYLLNKPRGVISSVSDDKGRKTVVDLLPTVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYLARVKGVANKEVLRPLTRGVVIDGKKTKP
                     AVYEIIKVDPVKNRSVVELTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGNLDLTGLRP
                     GEYRKLNKKDISKLHTLAVTKPVKK"
     gene            9320..9559
                     /gene="yidD"
                     /locus_tag="AC1PNG_001206"
     CDS             9320..9559
                     /gene="yidD"
                     /locus_tag="AC1PNG_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIP6701713.1"
                     /translation="MTRLLIGLVRFYQKFISPLFQPSCRYRPTCSAYMIEALQKHGLR
                     GFLMGLARIGRCHPFVEGGEDPVPDRFSLKRNKQK"
BASE COUNT         2740 a         1846 c         2244 g         2737 t
ORIGIN      
        1 tcgtttcttt ggcaagattt acggagtggt tgctatggga attggactat cagcattagt
       61 atcattccta gctgttacag tctttcaatc tttattgctt agtctgctga gtgcaggctc
      121 catcatcatg atgctgatta tgattggtca gattgccttg gttgtttctg cttcagcaat
      181 ggcagctaaa aatagcccaa tggctctacc aatgtttctt gcctactcag taacaaatgg
      241 gattaccatc agcatgatct tgatgttcta taccagcgaa acagttgttc ttgcctttgt
      301 gtcagcggct cttatgtttg ctatcatggc tgtaattggg atgacaacga agaaggatct
      361 ttctggtatg gctcaagcat tgcgtgcagc tctatgggga attattattg caagtgttat
      421 taatatcttc cttcgtagct ctggtctaag ctttatgatg tctatcattt ctgttttggt
      481 attctcaggt ttgattgctt atgacaacca acgtatccgc aatgttttcg aacaaactgg
      541 tggtaatgtt ggtcaaggtt gggtcgtatc tatggcgctt cagctctatc ttgactttat
      601 aaaccttttc ctcaacttac tccgtatctt tggtggattg agtcgagact aattatgaca
      661 tataaaaaag aggggtgccc tcttttttta tatgtcgttt gttatgctag agctcccatt
      721 ggatcccatg gggcaaggac ttgtggttga gcttgataag ctgctagttc ttcttgtgtg
      781 aggaattctt ttcggacaac aatctgataa gtgtactcgt ccatccagct atctgatgct
      841 acgaagtatc ccttgtcccc aaccttgtca ccccaagagt tttctacttt ccatttgaga
      901 ggttgctcat tatcgtctaa atcaacacca gttaagacca tagcgtgggt catcaagctt
      961 tcggagtagt ctaatcttcc agccttgtct tgatggaaat ggatacccag acctgaagag
     1021 aagtcgtagg tgttggttgc cataatacca gtttgacgat tgctactttg accgacatcg
     1081 gaaccaaacc aaacggactc accagctttg agttgagcaa tagctagttc tttaaaacgg
     1141 ttcatttcta cattgaggta gcggacagca ggggcaccaa caacgttccc caacaattcg
     1201 actgtataag atttattgta aggtttatca gtagttggag cattgataat ggagacatag
     1261 tcagatagtt tcaaaccaac gtatttctcg tagaatgcct gaggtgtcac atttgtattg
     1321 cggtgataga cattgtcttt atctctatat gcaaaatcaa agctgcgtgg agggagtcca
     1381 aggttgacgg ctaggaagtt aaagatttct tgaagcaagt tttctttttg tatttgaact
     1441 tctttcgagg aagctccttt ggcgaggagg tctcgcaaga tttgtgcatc ttgacggagc
     1501 aatttgttaa ggtactgatt gagctcacgg ctggcactgg atgagataga ttctggatag
     1561 acggatttcg gtaccacacc atatttttca aatagggcaa caaccatatc ccattgaccg
     1621 ccatcttgtt gcggggtatc caataagaat tttaccttac ggctaccaat ttcttggtca
     1681 gctgtggcaa tgatttgttc gaggaaccag ttggattttt catatttatc ccaaaagaag
     1741 gtatgggcct gtgatagctc aaaattctcc agtttgaaat cagagatgag cttgtggcgg
     1801 aaggtgttta atgcagcaaa catccagcaa cgtccagaag ccttttggtt tgaaacagcg
     1861 tccttggtca agtcaattga aaagacatag tcattgtcga tggcgctttg gcgtgtttct
     1921 aaggatttca aaagtccgtt gtggctaacg gcattttcgg ttgcctgtag gcttggatta
     1981 gctagatagt tggcatacaa gcgttctgta aaatcaaaat ctaatgttga catatattac
     2041 tcctttttta atgataatca cattatagcg ctcataattt attttgcaag tatttggtgg
     2101 agttgaaagc tacagttcct cacatatttg tgatataata ggaaaaatat ttgatgaggt
     2161 agtgacatga ccaagacacc atttgttagt aaagaagttt tagaaaccat tacggagcaa
     2221 tttccgactc cttttcattt gtatgatgaa aaaggcattc gtgagaaggc ccgtgccctc
     2281 aatgcagcat ttgcatggaa caaaggcttt aaggaatatt ttgcggtcaa ggcgacaccg
     2341 acacccgcta ttttgaaaat tttacaagaa gaaggttgtg gagtggactg tgcgacggat
     2401 gtagaagtac tcatgagtga aaaattgggc tttaaggaca tcatgttcac atcaaatgac
     2461 acccaagcgc aagagtttgt ctatgctcgt aaagttgggg cgaccattaa ccttgatgcc
     2521 tacgagcata ttgagttttt gaaaaatgtt gctggtatcc cagaaacggt ctgcctccgt
     2581 tacaatccag gtggggtctt ctcgctggga acggatatta tggaccaccc agaagagtct
     2641 aagtttggga tgaccaagga acagttgatg aaaggctata aggaactaaa agagcttggt
     2701 gtcaaggaat ttggtattca tgcctttcta gcttcgaata cagtaacaaa tgactactat
     2761 cctgtcttag ctcatcaact ttttgaattg gccttggaaa ttcgtgagga aacagatgtg
     2821 accttggact tcatcgacct atcaggtggg attggagtca attatcgtcc tgagcaagaa
     2881 ccaaacgaca ttgctgtcat tggtgagggt gtccgtaagg tttatgagga aattctcacg
     2941 ccagctggta tgggacatat taaaatcttt acagaattgg gtcgctttat gttggcgcca
     3001 cacggtcact tgattacaaa ggtgctccat cgtaaggaaa cctatcggac ttatataggt
     3061 gtggatgctt cggctgcaaa ccttatgcgt ccagccttct atggcgccta ccaccatatc
     3121 accaatatca cgcgcccaga tgcgccaatc gaagttgtgg atgtggcagg ttccctctgt
     3181 gaaaacaacg acaagtttgc ggttaatcgg gaattaccac gcgcagaagt aggagacacc
     3241 ttggtcattc atgacagtgg tgcccacggc ttctccatgg gctacaacta caacggtcgt
     3301 ctgcgttcct ctgaaatcct tttgcaggaa gatggcacag cgcgtatgat tcgtcgtgct
     3361 gaaacaccag aggattattt cgcgaccatt tacggttttg attttgacag gtaagtcttg
     3421 gaaaagcctg ctaaatttgg tataatagat tcattaagag attgtcgcag ggcaattccg
     3481 aatgaattat ttttagaaga gtcaggagat tgacaaatga acttaggttt agcaatttta
     3541 cttattgtat tggcatttgc aggtggtgta gccttgggta tttacttgtc acgcaaacaa
     3601 gtggaaaact acattgctga caagccagtt ttggatgaaa atgctttgcg tttgatgatg
     3661 agccaaatgg ggcaaaaacc aagcgaggca aaagtacaac aagtacttcg ccaaatcaag
     3721 agccaacaaa aagtggcaag caagaaaaaa taatcaaact gggactggga tattcattcc
     3781 cagatcccat tttttgagta gaaaagaaag gctagtcaga ttttctggct attctcagta
     3841 ttatggataa tcgaccaata ggttttttag attcaggtgt ggggggatta acggttgcgc
     3901 gtgagttaat gcgccaactt ccccatgaag aaattgttta tataggggac tcggcacggg
     3961 caccttatgg cccccgtcca gcggaacaaa tcagagaata tacttggcag ttggtcaatt
     4021 tccttttgac aaaaaatgta aaaatgattg tctttgcctg caatacggca acagcggtag
     4081 cctgggaaga agtcaaggaa aagttggata ttcctgtttt gggagttatt ttaccaggtg
     4141 cttcggcagc tattaaggcg acgcaaactg gtaaggttgg tgtcttagga acggctatga
     4201 ccattcaatc agatatttat cgacaaaaga ttcaggccct ttctccagaa acgcaggtag
     4261 acagcctagc ctgtcccaaa tttgcaccac tggttgagtc caatagccac caatccagtc
     4321 ttgccaaaaa ggttgtttat gagacacttc gaccactcgt tgggaaagtg gataccttgg
     4381 tcttgggatg tacccattat cccctacttc gccccatcat ccagaatgcc atgggcaagg
     4441 atgtcaagtt gattgacagt ggggcagagt gcgcgcgaga tatttctgtt ttattaaatt
     4501 attttcaaat caaccgcagt cgtaccgaaa aggatattca gcacagattt tacacaacag
     4561 ctagtccggc agcttttaaa gaaatagctg aaagctggat gggcattgat attcatgtgg
     4621 agcatgtaga actatgacag acaagatata cgaatacaga gatgaacaca attggtttat
     4681 cggaaaagcc agctttgcca atctatttgg ttcatttggt gaaaatggca gagagcagga
     4741 aatttaccag attggtcaat tatttgacaa actgattgct ggcaattatg aagacgagaa
     4801 ctttaaccag tgtgtcaaca ttgaagtgat taaacttcag tctgaatttg ccctctttca
     4861 atttgcctgt gatactttga atgagttaaa caatcgtcag ttcaaggttc tccaacacca
     4921 aggagctatt ctggtaactg agaatgacaa acttctctta gtccatttgc cccaagcagg
     4981 ggttagcacg gcagatttct tcggtcaaga caagggattg gcaagcgtag gagataccat
     5041 cttgatcgct acaaaaaatg aaggtaagac caaggaattc cgtaagtttt ttgagcgttt
     5101 tggctaccag gttgagaacc tcaacaacta tcctgacctg ccagatgtag cagaaactgg
     5161 tatgactttt gaggaaaatg cacggctcaa ggctgaaacc atagcagagc tgacaggcaa
     5221 gatggtcttg gcagatgatt ctggtttgaa ggttgatgct ttaggtggtt tgccaggagt
     5281 ttggtctgcc cgtttttcag gcccagatgc cacagatgag ctcaataatg ccaagctctt
     5341 gcacgaattg gccatggtat ttgaactgaa agaccgttcg gcacaattcc attgtactct
     5401 ggtcatggct gctccaaatc gtgatagttt ggttgtcgaa gcggactggg aaggctttat
     5461 cggtatggac ctccgtgggg aagatgggtt tggatatgat ccacttttcc tcgtaggtga
     5521 aacaggtaag acctcggcag aattaaccct agaagaaaaa aataagattt cccaccgtgc
     5581 acaagcacta gaaaaattag tggaggcatt tccagtatgg caggagcaag caaaacaatc
     5641 ctagtcatga gcgactctca tggggataga cagattgtag aggatattaa acatcattat
     5701 cttggcaagg tcgatgccat ctttcacaat ggtgattcgg agctggatag tcaagatagt
     5761 ctctgggatg gtattcaagt ggtaaatggc aactgcgact attttggcgg ttatccagac
     5821 cagctcatta ctcaattggg tgatgtgact atcgctcaga ctcatggtca tctttatggc
     5881 attaattacg gctggcagcg gttggattat tgggcacagg aagttgatgc ggatatttgt
     5941 ttgtatgggc atctgcatgt gcctgatgcc gaagtgcgtg gcaagaccct cttcctcaat
     6001 cctggctccg ttagtcaacc tcgcggtttg gtcagagagt gtctctatgc tcttgtgacc
     6061 atctatgatg atcacttcca tgtggattac tacaatcgac agcaccaact ttacccagca
     6121 ttgacaaagg atatttcaag atgattgcaa gagaatttga agtcttttta ttggaccagg
     6181 aaggaacttt cttgacacca gcggataaat tggcagtaat tattgatacg cataatattg
     6241 accatgcaaa actgttactc agtcacatga cctattcacg cgtacctgtg gtaacagaag
     6301 atggccgctt ttttggaacc atcggcttga cagaaatcat taaatatcaa gctgaaaatg
     6361 aactatcgga tgatgagtta aacagagata tttctgtgat tgtaaagaca gatgaagaaa
     6421 cggtaggtct ggattatgat ttgacagaag tcatgcgcaa gctcatcgac caatccttcc
     6481 taccagttct tggagaaaac agggaatttc tggggattat tacccgtaaa tctattctga
     6541 aagccatcaa tgcgcttttg cataattttc ccttggcatc aaaagaaggc aagaaatgaa
     6601 gcaggcgatt gaatctttta tccagagcaa aaaggtcagt gtcaacagcc aaaagtccta
     6661 tacctatgac ctgcagcagt ttgtgacagt aacaaagggg gagattagcc aacagtctct
     6721 tttggtctat cagcagtctc ttctagactt gaaacctgct gctcagaaaa gaaaaatgtc
     6781 agcggtcaat caatttctct actttcttta tgaaaataat ctcctggatc gtttttataa
     6841 attgcaaacg atgtcagaac cagcaagcgt caaaaagaaa ctggagcgtg aggatttaac
     6901 tctcctcttt caagaaagtc cttggcttga tgggcaactg attactcttt tgattgcctt
     6961 actagggctg actcctagtg aaatagcaga gctgactagt cagcaggtca atctggattt
     7021 tcaagtcctg acagttgaga agggaggagc caagcgggtg ctcactctgc ccaaggaatt
     7081 gataccctat atggagtctc atctgtctgg acgctatgtc ttcgataaga agggacagac
     7141 ctattctcgt caatggtttt ttaatcgtct gacagaattt gtccagtcca tcggcaagcc
     7201 tgattggaca gcccaaaaat tacgggagca gtatattcta aaacatatgg acgaaggaaa
     7261 atccttggac caaatcgcca agcagttggg actgaaaaca agcatgagtt tggaaaaatt
     7321 tagataatgg atataaaatt aaaagatttt gaagggccac ttgatctcct gctacacctg
     7381 gtgtccaagt atcaagtaga tatttacgag gttccgatta cagaggttat tgagcaatat
     7441 ttggcctaca ttgctacctt gcaagccatg cgtttagagg tagcgggaga atatatgctc
     7501 atggctagtc aattgatggt catcaaaagt cgtcggttat taccgaaggt tgttgagcaa
     7561 attgatcccg aagatgatcc tgaaatggac ctcttggacc agttggaaga ataccgcaag
     7621 ttcaagcttc tcagtgagaa attaggggag caacatgatg aacgggccaa ttatttttca
     7681 aaacccaaat tagatttgat ttatgatgat gtacagttag ctaaggataa gaccatcatt
     7741 gacattttcc tagctttttc taaagtaatg gctgaaaaac aggcaagcct ccgtcagtct
     7801 catgcgacca ttgcccgtga tgaatataaa atcgaagaca tgatggactt tgttcgcagt
     7861 cgtttcgaga caggtccacg ccttgaactc cgtcagcttt ttcaagaaag tcaagatgtc
     7921 aatgaaatga ttacgatctt ccttgcgact ttagagttgg tcaaggttca tgaaatcgtc
     7981 ttggagcaga cagagacttt tggagatatt tatctagtaa ggagtgaaga tgaatcgctt
     8041 agctgaaatc gaagtcctgc tctttgtggc aggtgaggat ggcttgacgt tacgaaattt
     8101 ggcagagatg ttggaaatgc agccgacagc tgtcagccag cagttagaga aacttagtga
     8161 gaaatatatg gcagacaaag cgtcaggctt agccatctta gagtcttcca atcgctacaa
     8221 gttggttact aaaaaagaat atgcaaacct tttgcgaatc tatgccaaaa caccaatcaa
     8281 tcagactatg tcacgggctc ttttggaaac cttatcaatc gtagcctata agcaacccat
     8341 tactcggatt gaagttgatg atattcgcgg agtcaattcc agcggtgcca tcagtaaatt
     8401 acaggccttt gacctcattc gtgagaacgg taagaaagag gttcttggta gaccaaatct
     8461 ctatgtgaca acggattatt tcctggatta catgggaatt aatagtctag aagaattgcc
     8521 tgatgtgtct gaaatagatt tgattcaaga agaaacagag ctattcgtag aaagaaatga
     8581 gttagaaaat gagaattaat aaatacattg cccatgctgg agttgccagt cgtcgtaagg
     8641 cagaagaact aatcaaacaa ggactagtta ctgtaaatgg tcaggttgtt cgtgagctag
     8701 ggacagttat caaatcgggt gataaggtag aggttgaggg aaaaccaatt tataacgaag
     8761 agaaggtcta ctatctcctc aataaaccac gaggtgttat ttctagtgtg tcagacgaca
     8821 agggaagaaa gacagttgtg gacttgttgc caacagttaa ggagcggatt tatccagttg
     8881 gacgtctgga ctgggataca tctggggtct tgattttgac caatgacggt gattttacag
     8941 acgaaatgat tcacccacgt aatgagattg ataaggttta cttggcgcgt gtcaaaggag
     9001 ttgccaacaa ggaagttttg cgtcccttga cccgtggtgt tgtcattgat ggcaaaaaga
     9061 ctaagcctgc ggtgtacgag attatcaagg tggaccctgt taagaaccga tcagttgtag
     9121 aattaacaat ccatgaaggt cgaaaccacc aggttaagaa gatgtttgag gctgtcggct
     9181 tgcaggtaga taaactgtct cgtactcgct ttggtaatct tgacttgacc ggcctacgtc
     9241 caggagaata ccgcaagctc aataagaaag acatcagtaa actacatacc ctggctgtta
     9301 ctaagccagt aaaaaaataa tgactagact tttaatcggc ttggtccgat tctatcagaa
     9361 atttatttcc cccctttttc agccctcttg tcgctaccgt ccaacttgtt cggcttatat
     9421 gatcgaggcc ttacaaaaac atggactcag gggctttttg atggggttgg ctcgaattgg
     9481 tcgctgtcat ccctttgtgg aaggtgggga agatcctgtg ccggacaggt ttagtttgaa
     9541 aagaaataag cagaagtagg gcaacct
//
LOCUS       DBNFEG010000075         9537 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.075, whole genome shotgun sequence.
ACCESSION   DBNFEG010000075 DBNFEG010000000
VERSION     DBNFEG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.075"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            14..955
                     /gene="manA"
                     /locus_tag="AC1PNG_001207"
     CDS             14..955
                     /gene="manA"
                     /locus_tag="AC1PNG_001207"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HIP6701714.1"
                     /translation="MEPLFLTPVMHEKIWGGNRLRTNYHYDIPSDKTGECWAISAHPN
                     GVTTVSNGQYKGRGLDDLYKNEKHLFGNPTDDVFPLLIKILDADDWLSVQVHPDDSYG
                     LAHEGELGKTECWYILEAEDGAEIIYGHNAQSKEELRQQIEAGDWDKLLTHVPVKKGD
                     FFFVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRRDDAGNLRELHIEKSIDVLTIG
                     PVANSTPAHLKAGNLDSTLLVANPFFTVYKWNIQQEIKMKQTVPYLLVSIIEGEGAIQ
                     VGETSYPLQKGSHFILPADVTDWTFTGQMDIIASHAN"
     gene            complement(1003..2394)
                     /locus_tag="AC1PNG_001208"
     CDS             complement(1003..2394)
                     /locus_tag="AC1PNG_001208"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIP6701715.1"
                     /translation="MKKFPTNFWWGAATSGPQSEGRFYKKHKNVFDYWYDIEPEAFYE
                     GVGPDVTSNFYNSFREDIAMMREVGLNSVRTSIQWTRLIDDLETNTVNQAAVEFYNQV
                     FDCFIENGIRPIVTLHHFDLPAELYHKYGGWESRRVVDLFVGFAEQAFKLFGDRVKDW
                     TTHNEPIVVVLGQYLQQFHYPKLVDGKKAVQVAYHLNLASALAIAKYRELGLDEEGGR
                     IGIVLNLTPTYPASQAPADLEAARIAELWNNKLFLEPAIHGHFPEELVDILSRDKVIW
                     KTEPGDDQILRDNTIDFLGVNFYHPNRVQAPAISPSSVGDWKPERYFDSYALPGRRMN
                     LDRGWEIYPEALYDIAITIRDHYNNIPWFVSENGMGISHEERFLDENGQVQDDYRIDF
                     IRDHLECLHHGIEEGSNCFGYHMWTPIDCWSWSNAYRNRYGLIATDIHTQVKTIKKSG
                     EWFRMIVSQNGLS"
     gene            complement(2408..3280)
                     /locus_tag="AC1PNG_001209"
     CDS             complement(2408..3280)
                     /locus_tag="AC1PNG_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIP6701716.1"
                     /translation="MTLLVCDLGGTTVKFGLYDKGRLFNKSSFRTPTTWEEMKSTILN
                     LKDHFSYRGLKGVALSCPGAVDTKKGIIYGRSAIPYIHRFPIQDELSALLELPVTIEN
                     DANCAALAELNLGVARNASSSVFFIIGTGVGGAIAQGRNLLRGEDHFGGEFGCMILRD
                     GLSMSKLVSPVRQARLFAQQYGYPDTFSGKELFTLAEFGDKRALEVITEMYDYLAIGI
                     YNVLVATNPELVVIGGGISARADLIEQIQQRLDALLVKTKMEDLTYRIEACEFRNDAN
                     LLGAALHFVERYGM"
     gene            complement(3385..4131)
                     /locus_tag="AC1PNG_001210"
     CDS             complement(3385..4131)
                     /locus_tag="AC1PNG_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6701717.1"
                     /translation="MEKKRVFLQVSMDDDFNEMNHREPAEEFCFYRAVAKGDLNAVRM
                     NCEQQRFLEMNGVGVLSKNPVTNIKYHFVITTAMITRFCGQNGLELEQAYRMSDFYIQ
                     KLDTIHTVQEVQSLHDEMVLHYTEKMRRYHQNDTNSKHVNACKEHIYKNLTERITVDA
                     IADELGVSSSYLSRLFKKEVGVSISTYIRDLKIESAKELLKYGEMSMIEIANHLSFSS
                     QSHFIQQFRDATGVTPKKYRDENAKLKWDR"
     gene            complement(4226..4891)
                     /gene="bglS"
                     /locus_tag="AC1PNG_001211"
     CDS             complement(4226..4891)
                     /gene="bglS"
                     /locus_tag="AC1PNG_001211"
                     /EC_number="3.2.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042972 - licheninase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucanase"
                     /protein_id="HIP6701718.1"
                     /translation="MTHSIENTHENNKVSYMETFQEYDSDTMELADWANGEMFGCRWS
                     PENVSFSVEGLRLTIAQDEKGELTGGEWRTRESFGFGKYEVSMKPIKNTGVVSSFFTY
                     TGPSDGTQWDEIDIEFLGYDTTKVQFNYFINGVGNHEYLYDLGFDASEAFHTYGFEWR
                     NDFITWFVDGEPVHTVTEDIPSTPSKIMMNVWPGTGVDEWLGPYDRAIPLVAEYQQIS
                     YLP"
     gene            complement(4909..6258)
                     /gene="bglS"
                     /locus_tag="AC1PNG_001212"
     CDS             complement(4909..6258)
                     /gene="bglS"
                     /locus_tag="AC1PNG_001212"
                     /EC_number="3.2.1.73"
                     /inference="COORDINATES: protein motif:HMM:NF047856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042972 - licheninase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucanase"
                     /protein_id="HIP6701719.1"
                     /translation="MSKKIVLSWLCVASTICSLAVNGKQILAEDVASQRLPTSEQIIS
                     DSSETTSLSTETVVAETEVAVTSQSDETSPATTASPENEMISEVVEETVEPSSEVETP
                     KQEAFSTDFDTFDESTAQLADWSNGAMFNARWTPSNVAFSDGIMTLTIDNDGKGGYNS
                     GEWRTQQYYHYGKYEVRMKPISNPGTVSSFFTYTGPSDGTQWDEIDIEFVGYDTTKIQ
                     FNYFTNGVGGHEHYYNLGFDASEEFHTYAFDWKEDSITWFVDGVAVHTATTDIPTTPG
                     KIMMNAWPGIGVDEWLKPFDGNVPLVASYDWMKYTPNTVELPESKIENDEQMTNTTPE
                     ETSEIKSDSNEGSSLSHEISDSNSSSTLHNDSNHQDENSVPSGQQQSNVTLEGKQEVK
                     KTQPLPLPNNNVNQTNLSNQHKLPNTSSQTGWLSMFVGSVLFIVATLVCSFTSRKRL"
     gene            complement(6281..7126)
                     /locus_tag="AC1PNG_001213"
     CDS             complement(6281..7126)
                     /locus_tag="AC1PNG_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003030789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIP6701720.1"
                     /translation="MSTNAQNHFRRIVAHFVLIVLSFMCLFFFYILIVSATHSHSELQ
                     RGFSALPGGYLWDNLMKVANDGTFPMFKGIINSLIVSGTTAAVCTYFSALTAYGLYVY
                     DFRFKKIAFTFIMAILVMPTQVTSMGFLRLITQMGMYDSFLPLIIPAIASPAVFYFMY
                     SYMKASLPLSMVDAARIDGSSEFRTFNTIVLPLMKPAIAVQAIFTFVGSWNNYFIPAL
                     VLQSKDKMTVPILIASLRGADYMNFDMGKIYTMILVAIVPIVLVYLFLSKFIIAGVTL
                     GGVKE"
     gene            complement(7138..8079)
                     /locus_tag="AC1PNG_001214"
     CDS             complement(7138..8079)
                     /locus_tag="AC1PNG_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIP6701721.1"
                     /translation="MKQKRRRINYGKWGYLFILPFFVTFFIFSFIPLVETVRYSFYEY
                     YRSGIREIGPTFIGLDNYISLLQSDILKYAGNTMLMWLMGFIPQIGIALLLANWFVDA
                     RLKLRGQGIYKVIIYLPNLIMASAFAMLFFTLFSTNGPINSIMTSLGIFKEPVDFMGT
                     SLGTRTLVAVMNFLMWFGNSTIMLMAAIMGINPDIFEAAEIDGCNSRQRFFYITLPMI
                     RPILAYTLITSIIGGLQMFDVPQILTNGQGAPDRTSTTLIMFLNNHLKSKNYGMAGAL
                     SVYLFVVSAFLCWFVYKMTNNELKLGRTKMKQKVKKV"
     gene            complement(8130..9503)
                     /locus_tag="AC1PNG_001215"
     CDS             complement(8130..9503)
                     /locus_tag="AC1PNG_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIP6701722.1"
                     /translation="MNKKVSALLLTALASTALVACGSKSESAGTSQSEEGKVINIYSW
                     NDEFRQRVEAVYPEVKETSADGTITTLKDGTEIHWIVNPNQDGVYQQKLDEALLNQES
                     AAADEKVDIFLSETDYVNKYSSKEADAALPLKELGIDPEKDLSDQYSFTKVTASDEDG
                     VQRGSTWQTTPGVIVYRRDIAKAVFGTDDPSEIGKKVEDWATAKETAKILKDKGYTIF
                     ASPADTFRLYGNSIKEPWVKAGETTINVAPEVMQWVKDSKEWLDAGYFTKTVKGQWTD
                     EWNKEMSSQAKNFAFLFPAWGIDFVLKPNWDGAEGSWAVTTPPQEYNWGGSYIHGAVG
                     TDNSKHVKDIILALTGNKENLLKISKDYLDFTNTQSGMQEAAKDENFKSDFLGGQNAF
                     EYFAPVAENIKIAPLSAYDQGSVELIQNNFNDYLQDKVSFEKAKENFEIAIKERYPEI
                     TEIKWPD"
BASE COUNT         2940 a         2065 c         1586 g         2946 t
ORIGIN      
        1 taaggagaac atcatggaac cattatttct cacacctgtt atgcacgaaa aaatttgggg
       61 tggcaatcgc ttaaggacca actaccacta cgacattcct agtgataaga ctggtgaatg
      121 ctgggctatt tctgcccacc caaacggcgt aactactgtc tcaaacggtc aatataaagg
      181 aagaggactg gatgacctct ataagaatga aaaacattta tttggcaatc caacagatga
      241 tgtctttcca ttattaataa aaatccttga tgcagacgat tggcttagcg tccaagttca
      301 tcccgatgat agctatggct tggctcacga aggggaactg ggcaagacag aatgttggta
      361 cattttggaa gccgaagacg gagctgagat catttatggt cacaatgccc aatccaaaga
      421 agaactccgt cagcaaatcg aagctggaga ttgggacaag ctgttgaccc atgtacccgt
      481 taaaaaaggt gatttcttct ttgtaccaag cggcaccatg cacgccatcg gtaaaggaat
      541 ccttatttta gaaacccaac aatctagtga tacaacctac cgtgtctatg actttgaccg
      601 ccgtgacgac gcaggcaacc tccgtgaact acacattgag aaatcgattg atgtactgac
      661 catcggacct gttgctaact caacacctgc ccacctcaaa gcaggcaatc ttgactcgac
      721 acttctggtt gccaatccct tctttaccgt atataaatgg aatatccaac aagaaattaa
      781 aatgaaacag acagtacctt acctactggt cagtatcatt gaaggggaag gtgccatcca
      841 agtcggtgaa accagctacc cacttcaaaa aggaagccac tttatcttac cagccgatgt
      901 gacagactgg acctttacag gccaaatgga catcatcgca agccacgcga actaaaaaat
      961 aaatgaaaac cgttagaata ctgccctaac ggttttattt ttttatgata gtccattctg
     1021 actgacaatc attctaaacc actctcctga ttttttaatg gtcttcacct gtgtatggat
     1081 atctgttgca atcagaccat aacgattccg ataagcattt gaccaagacc aacaatcaat
     1141 cggcgtccac atatgatagc caaaacaatt acttccttct tcaataccat ggtgtaagca
     1201 ttctagatga tctcggataa agtctatccg atagtcatct tgtacttgac cattctcatc
     1261 taaaaaacgt tcttcatgag aaatacccat accattctct gagacaaacc atggaatatt
     1321 attataatgg tcacgaatag tgattgcaat atcatacagt gcttctggat aaatttccca
     1381 accacgatcc aaattcattc tacgcccagg taatgcataa ctatcaaagt atcgttcagg
     1441 cttccagtcg cctactgaac ttggagaaat agctggagct tgaacccgat ttgggtgata
     1501 gaaatttacc cccaaaaaat cgatagtatt gtctcttaat atttggtcat cacctggttc
     1561 cgtcttccaa atgactttat ctcttgataa tatgtcaact aattcctctg gaaaatgccc
     1621 atggatggct ggttcaagaa acaatttatt attccacaat tcagcaatcc gtgctgcttc
     1681 caaatctgct ggtgcttgtg aggctggata agtcggagtc aagtttagaa caattccaat
     1741 acgacctcct tcttcatcaa gacctaattc acgatatttt gcaattgcta gtgccgaagc
     1801 aagattcaaa tggtaagcga cttgtacagc tttttttcca tcaactagtt ttggatagtg
     1861 aaactgttgc agatattgac ctaacacaac aacaattggc tcattatgag ttgtccaatc
     1921 ctttactcga tcaccaaaga gtttgaatgc ttgttcagca aaaccaacaa acaggtcaac
     1981 aacacgtcga gattcccaac ctccatattt atgatacaat tctgctggta gatcaaagtg
     2041 atgcaatgtt acaattggtc taatcccatt ttcaataaaa cagtcaaaca cctgattata
     2101 aaactcaaca gcagcctgat tgacagtatt ggtttccaaa tcatcaatca gacgagtcca
     2161 ctgaatagac gttcgaacac tattaagtcc aacttctctc atcattgcaa tatcttcacg
     2221 gaaactgttg tagaaattcg aggttacatc tggtccaaca ccttcgtaaa aagcttctgg
     2281 ctcaatatca taccaataat caaaaacatt cttgtgtttc ttataaaaac gtccctcact
     2341 ttgcggacct gaagttgcag ccccccacca aaaattagtt ggaaattttt tcattattta
     2401 ctccttttta cataccatat ctttcgacaa aatgcaatgc tgctccaagc aaattggcat
     2461 catttctaaa ttcacaagcc tcaatacgat aggttaaatc ctccatcttc gttttcacta
     2521 gtaaggcatc caatcgttgc tggatttgtt caatcaggtc tgctctagct gaaattccac
     2581 ctccaatcac tactaattct ggattagtag ccaccaacac attgtagatg cctattgcca
     2641 ggtaatcata catttctgtt attacctcta gagccctctt gtcaccaaat tcagcaaggg
     2701 taaataactc tttacctgaa aacgtatctg gatagccgta ttgttgagca aataatcgtg
     2761 cttgacgaac tggactaacc aacttgctca tactcaaacc atcacgcaaa atcatacatc
     2821 caaactctcc tccgaaatga tcttctcctc taagtaagtt cctaccttga gcaatagccc
     2881 caccaacccc tgtaccaatg ataaaaaaaa cggaagagct cgcatttcgg gcaacaccta
     2941 gatttagttc tgctaaggca gcacagtttg catcattttc aattgtcaca ggtagttcca
     3001 ataatgcact caactcatct tgaatcggaa aacgatgaat atagggaatg gcacttctgc
     3061 cgtaaataat ccccttttta gtatcaactg ctcccggaca agataatgca actcctttca
     3121 gtcctctata ggaaaaatgg tcctttagat ttagtatggt tgacttcatt tcttcccaag
     3181 tggttggtgt ccgaaatgaa gatttattaa agagtcttcc tttatcataa agaccaaatt
     3241 tgactgttgt tcctcccaaa tcacatacta ataatgtcat attcttcttc tttctatttc
     3301 tcattgaaga atatccacaa aatccattca atacatgata gaaaaaagcc tgagatactt
     3361 tcaagctttt atcctattga caacctacct atcccacttc aactttgcat tttcatctct
     3421 atacttctta ggtgtcactc cagttgcatc tctaaattgt tggatgaaat gactttgtga
     3481 ggaaaaagaa aggtggtttg caatttcaat catggacatt tctccatatt tcagcaattc
     3541 ctttgcagat tcaattttta aatctcgaat ataagtacta attgagacac ctacctcttt
     3601 tttaaaaaga cgagataagt aactcgatga tactcctagt tcatctgcaa tggcatcaac
     3661 tgtaatccgt tctgttagat ttttataaat atgctcctta catgcattga catgttttga
     3721 attggtatca ttttgatggt atcgtcgcat cttttctgta tagtgcaaca ccatttcatc
     3781 atgcaaactt tgcacttctt gtacggtgtg aattgtatct aatttctgaa tataaaagtc
     3841 actcatgcga tacgcttgct ctaactccaa tccattttgt ccacaaaatc gagtaatcat
     3901 cgctgtcgtt atgacaaagt gatacttgat attggtcaca ggattctttg ataaaacacc
     3961 gactccattc atctctaaaa agcgttgttg ctcacaattc attcgaaccg cgtttaaatc
     4021 tccctttgct accgcacggt aaaaacagaa ttcttccgcc ggttctcgat gattcatttc
     4081 attgaaatcg tcatccatcg aaacctgtaa aaataccctc ttcttttcca tactcattct
     4141 ccactcgaat tatttaagga atgctttacg ctttcattat atcatttttt gctttggaag
     4201 aacaagttga aatttacatg tatatttacg gcaaatagga aatttgctga tattcagcta
     4261 caagaggtat tgccctatca taaggaccta accattcatc tacacccgtt ccaggccata
     4321 cattcatcat aattttgctt ggtgtacttg gaatatcttc agttactgta tgaacaggct
     4381 ctccatctac aaaccaggtg ataaaatcat tacgccattc aaatccatag gtgtgaaacg
     4441 cctcactagc atcaaaacca agatcatata gatattcatg atttcctaca ccattgatga
     4501 agtaattaaa ttgaaccttt gttgtatcat accctaaaaa ttcaatatca atttcatccc
     4561 attgtgttcc atcgcttgga ccagtgtaag taaaaaatga agaaacgaca ccagtatttt
     4621 tgataggctt cattgaaact tcatatttac caaatccgaa agattcacga gtacgccatt
     4681 ctccacctgt aagctctcct ttctcgtctt gagcaatggt gagacgtaga ccttcaacac
     4741 taaatgagac attttcggga gaccaacgac agccaaacat ttcaccattt gcccaatctg
     4801 ctaactccat cgtatctgaa tcatattctt gaaacgtctc catgtaagac actttattat
     4861 tttcatgtgt gttttctatc gaatgtgtca tctttcacct tctcttttct ataagcgttt
     4921 tcgactagtg aatgagcata ctaacgtagc tacaataaat aatacactcc ctacgaacat
     4981 agataaccat cccgtttggc ttgaggtatt tggaagttta tgttggttgc ttagattagt
     5041 ctgattaaca ttgttattcg gtaatgggag aggttgagtt tttttcacct cctgcttgcc
     5101 ttccaacgtt acattggatt gttgctgacc tgaaggaaca ctattctcat cttggtggtt
     5161 tgaatcatta tgtaaagttg atgaactgtt tgaatcggat atttcatggg aaagtgaaga
     5221 accttcattt gaatccgact tgatttctga agtctcttca ggtgtcgtat ttgtcatctg
     5281 ttcatcattc tctattttag attccggtaa ttcaactgta ttcggtgtat acttcatcca
     5341 atcataagaa gcaaccaaag gcacgtttcc atcaaatggt tttagccatt catccacacc
     5401 aataccaggc caggcattca tcataatttt tccaggtgta gttggaatat ctgtggttgc
     5461 tgtatgaact gccacaccat ctacaaacca tgtaatagaa tcctctttcc aatcaaacgc
     5521 ataggtatga aattcttcag aggcgtcaaa tcctaaattg taataatgtt catgaccacc
     5581 tacaccattg gtaaaatagt tgaactgaat ttttgttgta tcatatccaa caaactcaat
     5641 atcaatttca tcccattgtg tgccatcact tggacccgta taagtaaaga aggaagaaac
     5701 tgtcccagga ttactgattg gtttcatgcg tacttcatat tttccataat ggtaatattg
     5761 ttgggtacgc cattcaccac tattgtaacc accttttcca tcattatcta tcgtaagtgt
     5821 cataatacca tctgaaaacg ccacatttga aggtgtccat cgtgcattga acattgcacc
     5881 attagaccaa tctgccaact gagccgtact ctcatcgaaa gtatcaaaat cagttgaaaa
     5941 tgcttcttgc ttaggagttt caacttctga tgatggttcc acggtctcct ctactacttc
     6001 tgaaatcatt tcattctctg ggctagctgt tgtagcaggt gaggtttcat ctgattgact
     6061 tgttacagct acttcagttt cggctactac agtttccgta ctcaatgatg tagtttcaga
     6121 agaatccgat ataatttgtt ctgaagttgg taatcgttga ctagccacat cttcagctaa
     6181 aatctgtttc ccattaactg ctaagctaca gatagttgat gcaacacaca accaagatag
     6241 aactattttc tttgacataa actgtctcct taaaattgtt ttattctttg acaccaccaa
     6301 gagtcactcc agcgatgata aatttagaaa ggaagaggta aaccagtaca attggtacga
     6361 tggcaactaa aatcatagta taaatttttc ccatatcaaa attcatatag tctgcaccac
     6421 gaagggacgc aattaatatc ggaacggtca ttttatcctt agattgaaga acaagagctg
     6481 ggatgaagta attattccat gaacctacaa atgtaaagat tgcttgaact gcgatagctg
     6541 gtttcatcag aggaagaaca atcgtattaa aagtacgaaa ttcacttgaa ccatcaattc
     6601 gcgctgcatc aaccattgac agtggtaaag aggccttcat atagctatac ataaagtaga
     6661 agaccgctgg cgaggcaata gcaggaataa ttaatggaag aaaactatca tacataccca
     6721 tttgggtaat caatctcagg aaacccattg aagttacttg agtcggcata actagaatag
     6781 ccatgataaa agtaaaggct atttttttaa atctaaagtc atacacataa agcccatagg
     6841 ctgttaaggc tgagaaataa gtacaaaccg ctgcagtagt accagaaaca atcaaactat
     6901 tgataatccc tttaaacatt gggaaagtcc catcattagc aactttcatc agattatccc
     6961 aaagatatcc cccagggagg gcagaaaatc ctctttgcaa ctctgaatga gaatgtgtag
     7021 cactaacaat cagaatataa aagaagaaaa ggcacataaa tgataggaca atcaatacaa
     7081 aatgtgccac aatacgccga aagtgattct gagcatttgt tgacatattc taacctccta
     7141 tacttttttt actttttgtt tcatctttgt acgcccaagc tttaattcat tatttgtcat
     7201 cttatagaca aaccaacaca agaacgcact aactacgaac agataaacag atagtgcacc
     7261 agccatgcca tagttcttac tcttcaagtg attatttaaa aacataatga gagttgtaga
     7321 agttcggtca ggagctcctt gtccattagt cagaatttgt ggaacatcaa acatttgaag
     7381 ccctccaata atggatgtaa tcaacgtata tgctagaatc ggacgaatca ttggtagcgt
     7441 aatgtaaaag aaacgttgcc ggctattaca accatcaatc tctgctgctt caaaaatatc
     7501 tggattaatc cccattattg ccgccattag catgattgtt gaattaccga accacatcag
     7561 aaaattcatc accgcaacta aagtacgggt gccaagagag gtacccataa aatcaactgg
     7621 ttccttaaat atacctaatg aagtcattat cgaattaata ggaccgtttg ttgaaaaaag
     7681 ggtaaagaaa agcatggcaa atgcagaagc cataattaaa tttggcaagt atataattac
     7741 cttataaatt ccttgtcccc gtaatttaag tcttgcatca acaaaccagt ttgctaaaag
     7801 cagagcaatt ccaatctgag gaataaagcc cataagccac attaacatgg tattgccagc
     7861 atattttaga atatcggact gtaataaact gatataatta tcaagaccaa taaaggtcgg
     7921 cccaatttct ctaattcctg atcgatagta ttcataaaaa ctatatcgca cagtttcaac
     7981 taatggaatg aaggaaaaaa taaagaaggt gacaaaaaac ggtaagataa acagataacc
     8041 ccatttcccg taatttatac gtctacgttt ttgtttcatt ctgttactcc ttttagagta
     8101 aggcggtgca acatgcaccg ctactctctt taatcaggcc atttaatctc tgtgatttct
     8161 ggataacgct ccttaatggc aatttcaaag ttttcttttg ctttttcaaa gctaacctta
     8221 tcttgtagat aatcattaaa gttattctga atcaattcta cgctaccttg gtcgtaggca
     8281 gataacggag caatcttaat attttcagca actggagcaa aatattcaaa ggcattttgt
     8341 ccaccaagga agtcagattt gaaattttca tctttcgctg cttcttgcat accagattga
     8401 gtatttgtaa agtcaagata atcttttgaa attttcaaga gattttcttt atttcctgtt
     8461 aaagctaaaa taatatcttt tacatgttta gaattgtctg taccaactgc tccatggata
     8521 taagaaccac cccagttgta ttcttgtggt ggtgtcgtca ctgcccaaga accttcagca
     8581 ccatcccagt tcggtttcaa gacaaaatca ataccccatg ctgggaatag gaacgcaaaa
     8641 ttctttgctt gggagctcat ttccttgttc cattcatcgg tccactgacc tttaactgtc
     8701 ttggtaaagt agccggcatc taaccattct ttagagtctt taacccattg cataacctct
     8761 ggtgcaacat taatagtggt ttctccagct tttacccaag gttctttgat actatttcca
     8821 tatagacgga aagtatcggc tggagaagca aaaattgtat accctttatc tttcaatatt
     8881 tttgctgtct ctttagcagt tgcccaatcc tcaaccttct taccaatttc agaaggatca
     8941 tctgtaccaa aaacagcttt tgctatatca cggcggtaca cgattacccc tggtgtagtt
     9001 tgccatgtcg aaccacgttg aactccgtct tcatcagagg ctgttacttt agtaaaacta
     9061 tattggtcag ataggtcttt ttctggatca attccaagtt ccttcaaagg aagagctgca
     9121 tcagcctctt tagatgagta tttgttaaca tagtcagtct cagagagaaa aatatcaact
     9181 ttttcatctg ccgcagctga ttcttgattt agtaatgctt cgtccaattt ttgttggtaa
     9241 acaccatctt ggttcggatt gacaatccaa tgaatctctg tcccatcctt taaggttgta
     9301 atggttccgt ctgcagaggt ttccttcact tcaggatata ctgcctctac acgttgacga
     9361 aattcgtcat tccaagaata gatatttatt actttacctt cttctgattg gcttgtacca
     9421 gctgattcgg acttggatcc gcaggcaaca agtgcagttg atgctaacgc agtcaataat
     9481 aaagcgctta cttttttgtt catgtttctt ctccttttag aagttttctt tgttttt
//
LOCUS       DBNFEG010000076         9334 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.076, whole genome shotgun sequence.
ACCESSION   DBNFEG010000076 DBNFEG010000000
VERSION     DBNFEG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9334
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.076"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1167
                     /locus_tag="AC1PNG_001216"
     CDS             <1..1167
                     /locus_tag="AC1PNG_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP6701723.1"
                     /translation="MKKQSPFIIAGIVMLGVVMRAPFTALPAILIDVAAGLRVEVSSL
                     GILTSIPLIMFALCSSLAPRLAAKFGMEKLMALVLLVMVLGSGMRVLNLPALYIGTML
                     VGATIAFINVLLPSLVAANFPKKIGLYTTIYITLMGVAATVASMIAVPIVSSSSWEFF
                     ILLITGLVFMAFLIWLPNVRNNHRFASENQGNQKSSIWKNKAAIAFLIFGGLQSVLYY
                     TEITWLPTISQSVGFSKAEAGLMAGFFNMTAIPMSMIIPAVLSRQTKEMRRNIMLAIS
                     SVTLLGLVMMVLIPTNLILWSALHIILSFSNAALFPYMMLSFTLKTSNSQATAQLSGM
                     VQTGGYLIAAFGPGLLGYSYPIFGNWMPLILALAIVTLAMMWTIVLIEKEDIIL"
     gene            complement(1266..1436)
                     /locus_tag="AC1PNG_001217"
     CDS             complement(1266..1436)
                     /locus_tag="AC1PNG_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HIP6701724.1"
                     /translation="MAKGFGKGFLTGVLSTVALAAGAVFTVHKTIIEPEEKKEAFIEE
                     NRKKAARRRVAH"
     gene            1505..2389
                     /gene="miaA"
                     /locus_tag="AC1PNG_001218"
     CDS             1505..2389
                     /gene="miaA"
                     /locus_tag="AC1PNG_001218"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIP6701725.1"
                     /translation="MKTKVIVVIGPTAVGKTALGIDLAQRYNGEIISGDSQQVYRKLD
                     IGTAKASPEEQAAAVHHLIDVRDVTEGYSAYEFVAEAKALIADITSRGKLPIIVGGTG
                     LYIQSLLEGYHLGGQVDQVQVLAYRAELDSLSDEDLETMAEQAGLMVEGNSRRRIIRG
                     LELKKFGENLENTESGYEPLYICLTDDRQVLYDRINQRVDKMMAAGLLDEVSWLYQEH
                     PQAQAAMGIGYKEFFPYLAGAISLEEAIDKVKQNSRRFAKRQLTWFRNRMQVPFYSVG
                     EPDCKSQIFTAVEEFLND"
     gene            2382..3632
                     /gene="hflX"
                     /locus_tag="AC1PNG_001219"
     CDS             2382..3632
                     /gene="hflX"
                     /locus_tag="AC1PNG_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIP6701726.1"
                     /translation="MIETQKEQERALLVGVELQQTDNFDMSMEELASLAKTAGALVKG
                     VYTQKREKYDSKTFVGSGKLDEIAQMVEADEIDTVIVNNRLTPRQNVNLEEILGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRNQIHDIERQLKTVEKNRATVRERRLQSGVFKIGLIGYTNAGKSTIMNVM
                     TDKRQYEADELFATLDATTKQINLADKFNVTLTDTVGFIQDLPTELISAFKSTLEESM
                     NVDLLLHIIDASDPNHSEQEQVVLDILKDLDMLDIPRLALYNKLDKTDDSFTPSQFPH
                     VMLSAKDENAKGHIQMMVLAKIKTMFERFEVRVPLAESYKLHDLATLALIENRTYEED
                     VEVVSGYIASANKWKLEEFYDGLS"
     gene            3616..4245
                     /locus_tag="AC1PNG_001220"
     CDS             3616..4245
                     /locus_tag="AC1PNG_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HIP6701727.1"
                     /translation="MTDYLDLAMKYGGFTSLDKVYLANKLAGLTDQQKLDFITPPPSV
                     INAYFAEIYQKQGAEEATDYYLTLSQQLCLFPKEPSFAESKPFVRLNLSGKSFGFTYV
                     SADGLAQVFSEGVEEVTESLLFEIAQVFPHYVVFVEEGKIFMKVNPFEDAELEDVETD
                     YLLTQVATTEGLVKISGFNQEEVLEVADGYAGQYYYVWSGRSAILYIKH"
     gene            4256..5185
                     /gene="rnz"
                     /locus_tag="AC1PNG_001221"
     CDS             4256..5185
                     /gene="rnz"
                     /locus_tag="AC1PNG_001221"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HIP6701728.1"
                     /translation="MQIQFLGTGAGQPSKARNVSSLALKLLDEINQVWLFDCGEGTQN
                     QILETTIRPRKVAKIFITHLHGDHIFGLPGFLSSRSFQSSDEQTDIDIYGPVGIRSFV
                     LASLKVSGTRLPYRIHFHEFDVDTVGQVLETDKFTVFAEKLDHTIPCVGYRVIQKDLE
                     GTLDAEALRAAGVPFGPLFGKIKNGQNVTLEDGTEIIASDYISPPRPGKVVTILGDTR
                     KCHASVRLAVNADVLVHEATYGKGDEKIARKHGHSTNMEAAQVAKDAGVKQLLLNHIS
                     PRFLSKDISQLRKDASTIFEQVHIVKDLEEIEL"
     gene            5182..5937
                     /locus_tag="AC1PNG_001222"
     CDS             5182..5937
                     /locus_tag="AC1PNG_001222"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIP6701729.1"
                     /translation="MRTILITGASGGLVQEMVPLLKDDFLILLGRDVAKIEQLYVHHE
                     KKAVFDVDIRDEMALTAFFDELDSQFGQIDILVNNAGYAIYDDFENFSSQQVQDMFDI
                     NTFALMTMCRLVGKRMKVRRSGQIINIISMSGLIASAKSSVYSATKFAAMGFSNTIRL
                     ELAQYGVTVTTVNPGPIATGFFGQADPDGSYQESVKAFLLQPDYVAKKIVSAMGTKKR
                     DINLPWSLAAAHKLYTLFPRIADYLASTVFNLK"
     gene            complement(5975..9160)
                     /locus_tag="AC1PNG_001223"
     CDS             complement(5975..9160)
                     /locus_tag="AC1PNG_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HIP6701730.1"
                     /translation="MKLSYKKRLLNQVLLASTVLFATSLAQGTVFANTEEIPPTTSET
                     VTPLPEETPITKTSTSEATDNLVEGKETEKQTEEIADTSPALVSTEEETTSSEPNAEE
                     TTLRSANNDNQDTTEEKSAVPTIDTVTLETKTVYLSEAVTITESVTLPDTTEKIEWTL
                     DGKPISEWKTWNLKEGDFTGDTFITVEESRQDNQLHLNIQLAALFGEDLSKRTPSNIR
                     RTYRHFIKDMLLEGTSADGNLIISKTLHFRPYEAYRTHEEMLTEIEETKNNAATDRLV
                     RIESIGQSAEGRDIKMAVVAKDQASIDKYLTETTPLMLTQPDQMLKQLQAGTFDYKLP
                     ILINNTHADEQPGIDVVTSLFKEFAQKDTITFPSTDADGNPVTLHLKVTDLLDKFIFL
                     FNFTENPDGDVKNLRSLVNGLDPNRDTGFQVNPETQAIVRQIHKWNPISVLDIHGFVS
                     GFLIEPATPPHDPNFEYDLLADIMLEKAHEMGRAGIANSKYERYTIPNVHWGDGWDDS
                     FSGYTAVYAMYHGILGHTIEIPEGNQESFKAGFFAVLGGVHNMATKPDSLMEMRLKYY
                     SRGVNKVEDPKAESELVGPDGAVVGRVKKDQPKFFPDYYVIPMTLDKHNDMQEAFKMI
                     EYFNRNGVVVKELTEDVGNFRKGDLVVDMAQAKRGFANHVLYAGSDESAWGAMYAELV
                     VNFPDMKGFRAKAVFEENAFSSKLGSITWTKAPRTTEIDFKAPYYVVTNTSESAVQAI
                     NQAIKSGAKVYLTDDGYILETNQFSHLLDTYALYGEPLYKKPIGQELKAMKVYAPSHS
                     YSWAGDFAILANAALAVERMGFEIVNSADEADAIILESDQFDASVFGKKPIIIVGGVA
                     MQKLEELGILAGFNAEQFTDGGDYEGLMRAIIDDKDPLTSGYAMNGLFYSNSGNWIEG
                     IPEGFKTLVKIADKDYYIAGWWPGHDKLANKIVAIAGNYQDQPVFIYAGNPTNKVHPV
                     HFFRWVSNALFGSQLATLEDLPAVEILVPQPEMPKNILDEAPKNTTVVHTTKSTEAKQ
                     LPQTGEKTNYIAIALGSLLLGSIALRRKERS"
BASE COUNT         2495 a         1899 c         2138 g         2802 t
ORIGIN      
        1 atgaaaaaac aatcaccatt tataattgca ggaatcgtca tgctaggggt cgtgatgcgt
       61 gcccccttta cagctttgcc tgctattttg atagatgtgg cagcaggact gagagtagaa
      121 gtgagttcat taggaatttt aacctctatt ccgctcatca tgtttgctct ctgttcatcc
      181 ttagcaccgc gtcttgcggc taagtttggc atggaaaaac taatggccct agtcttactg
      241 gttatggtgc tgggttcagg aatgcgagtt ctcaacttac cagcccttta cattggtaca
      301 atgcttgttg gtgctacaat agcatttatt aatgttttgc taccaagttt ggttgcggct
      361 aattttccaa agaaaattgg tctgtatacg acgatttata tcactctcat gggggtggcg
      421 gcaacagttg catctatgat tgctgttcca attgtatcct ctagttcttg ggaatttttt
      481 attctattaa ttacaggatt ggtgtttatg gctttcctaa tctggttgcc taatgtaaga
      541 aataaccatc gatttgcaag tgagaaccaa ggaaaccaga aatcatctat ctggaaaaat
      601 aaagcagcga ttgccttttt gatatttggt gggctacaat cagtcctcta ctatacagag
      661 attacctggt tgccaactat ttctcaatca gttggcttta gtaaggcaga agcagggcta
      721 atggctggct tcttcaatat gacagctatt cccatgtcta tgattatccc agctgttctt
      781 tcccgtcaga caaaagaaat gcgtcgaaat atcatgctgg ctatttcatc tgttacctta
      841 cttggtttgg tcatgatggt attgattccg accaatctca ttctttggtc agcgcttcac
      901 attattttaa gtttttcaaa tgcagccctc ttcccttata tgatgttgag ctttactttg
      961 aaaacaagca acagccaggc tactgcccag ttatcaggaa tggtgcagac aggtggctat
     1021 ctcattgcag catttggacc aggccttctc ggttacagct atccaatctt cggaaactgg
     1081 atgccattga ttcttgctct ggctatcgtc acacttgcca tgatgtggac tattgttctc
     1141 attgaaaaag aagatattat cttataaagt ataatccctt aagaaggctg gcatttttct
     1201 gtgaaaatgg cagagcttct aagggatttt ttgtcaaaca aaaaagctag acatttgcca
     1261 gctttttaat gtgccacacg gcgacgtgca gcttttttgc gattttcttc gatgaaagct
     1321 tctttctttt cttctggttc aatgattgtt ttgtgaacag tgaagactgc gcctgctgca
     1381 agagcaactg tagaaagaac acctgtcaag aaacctttac caaaaccttt agccatagtt
     1441 atttctcctt tacttgtgtt ataatagatt ttagcgaaaa aacaaaatat tttcaaggaa
     1501 aaagatgaaa actaaagtta ttgtggtcat tggaccgaca gcggtaggaa aaactgccct
     1561 tggcatagac ttggcccagc gctacaatgg agaaattatt agcggtgata gccagcaagt
     1621 ctatcgcaaa cttgatattg gcacggcaaa agctagtcct gaagagcagg ctgctgcggt
     1681 tcatcacttg attgatgtcc gagatgtgac cgagggctat tcggcttatg aatttgtagc
     1741 ggaagccaag gccttaattg cagacattac tagtcgtgga aagttgccta ttatcgtggg
     1801 tgggacaggg ctttacatac aaagtctgtt agaaggctac catctgggcg gtcaagttga
     1861 ccaggtgcaa gttcttgctt atcgggcaga acttgatagc ttgtctgacg aggatttgga
     1921 aacaatggca gagcaggcag gtttgatggt cgagggaaac agccgacgga gaatcattcg
     1981 tggactggaa ttgaaaaaat ttggcgaaaa cttagaaaat acagagtcag gatatgagcc
     2041 actttacatc tgtttgacgg atgacagaca ggttctttac gaccgcatca atcagcgagt
     2101 ggataagatg atggcggcgg gcttactgga tgaagttagc tggctctacc aagaacatcc
     2161 ccaagcccaa gcagccatgg ggattggcta caaagaattt ttcccttatc ttgcaggagc
     2221 catttcgctg gaagaggcta ttgataaggt caagcaaaac agccgccgct ttgccaaacg
     2281 ccaattgact tggttcagaa atcgcatgca ggttcccttc tattcagtag gcgagccaga
     2341 ttgcaagtcg cagattttca ccgccgtgga ggaattttta aatgattgaa acgcagaaag
     2401 aacaagaacg cgccctcctg gttggagtgg aattacagca gacagacaac tttgacatgt
     2461 ccatggagga gttggcaagt cttgctaaga cggctggtgc tctggtcaag ggagtctaca
     2521 ctcaaaaacg agaaaaatac gacagcaaga cttttgtcgg ttctggtaag ctggacgaaa
     2581 ttgcccagat ggtcgaagct gacgagattg atactgtcat cgtcaacaat cgattaacac
     2641 cccgccagaa tgtcaatctg gaagaaattc taggtgtcaa ggtaatcgat cgcatgcagt
     2701 tgatcttgga tatttttgcc atgcgggcac ggagtcacga agggaaattg caggtccacc
     2761 tagcccagct caagtacatg ttgccacgct tggttggtca gggcatcatg ctcagccgtc
     2821 aggcaggtgg tatcggctct cgtggaccag gtgaaagcca gttggagctt aaccgccgga
     2881 gcattcgtaa ccagattcat gacattgagc gccaactcaa aacagtggag aagaaccgag
     2941 cgactgtccg tgaacgccgt ttgcaatcag gcgtgttcaa aatcggcttg attggctata
     3001 ccaacgcggg taagtccact attatgaatg tcatgacgga caagcgacag tacgaggctg
     3061 atgaactttt tgccacacta gatgccacaa ccaagcagat taacttggca gacaaattta
     3121 atgtgacctt gaccgatacg gttggtttca ttcaggacct gccgactgag ttgatttctg
     3181 ccttcaagtc caccttggag gagtccatga acgtggatct cttgctccat atcatcgacg
     3241 cctctgaccc caatcacagt gagcaggagc aggtggtctt ggacattcta aaagacctgg
     3301 atatgctgga cattccacgt ctagctctct acaacaagtt ggacaagacc gatgacagct
     3361 ttacccccag tcagttccca catgtcatgc tgtccgctaa ggatgaaaat gccaaaggtc
     3421 atattcagat gatggtcttg gccaagatta agaccatgtt tgaacgattt gaggtcaggg
     3481 ttccacttgc tgaatcttat aaattgcatg atttagcaac tcttgccctg atagaaaatc
     3541 ggacctatga agaggatgtg gaagtggtat caggctatat cgcttccgct aataaatgga
     3601 agttggaaga attttatgac ggattatctt gatttggcaa tgaaatacgg tggctttacc
     3661 agtctggaca aggtctatct ggcgaataaa ttagctggtt tgacagacca acaaaaacta
     3721 gattttatca cgccaccgcc ttcggttatc aatgcctatt ttgcggaaat ctaccagaaa
     3781 caaggggcag aagaggcgac ggactactat ctcactcttt ctcagcagct ctgccttttt
     3841 ccaaaagaac ccagctttgc tgaaagcaag ccctttgtcc gcctgaattt atcaggtaag
     3901 tctttcggct ttacctatgt gtcggcggac ggtctggccc aggttttttc ggaaggtgta
     3961 gaggaagtga cggaaagttt gctctttgaa atcgcccaag tctttcccca ctatgtcgtc
     4021 tttgtggagg agggcaagat tttcatgaaa gtcaatccct ttgaggacgc cgagttagag
     4081 gacgttgaga cagactacct tttgacccaa gtggcgacga cagaaggttt ggtcaagatt
     4141 tcaggcttta atcaggaaga agtgctggag gtggcggacg gctatgctgg tcagtattac
     4201 tatgtctggt cgggtcgctc tgcaatttta tatataaaac attagaaagt aaacaatgca
     4261 aattcaattt ttaggtacgg gggctggtca gccctccaag gctcgaaatg tatccagttt
     4321 ggccttgaaa ctcttggatg aaatcaatca ggtttggctc tttgattgtg gtgaaggaac
     4381 tcaaaatcaa attttagaaa cgactattcg tccacgtaag gtcgcaaaaa tctttatcac
     4441 tcacctgcac ggagaccata tttttggact gcctggtttt ttgtctagcc gttctttcca
     4501 gtctagcgat gagcagacgg atattgatat ttacggtcct gttggcatcc gttcctttgt
     4561 cttggcaagt ttgaaggtat cagggacacg cctaccttat cgcattcatt ttcatgagtt
     4621 tgatgttgat acggtcggcc aggtcctaga aacggacaaa ttcacggtat tcgctgaaaa
     4681 gttggaccat accattccgt gtgtcggcta tcgtgtcatc cagaaagatt tggaagggac
     4741 attggatgca gaggctctgc gcgcggcagg tgtgccattt ggtcccttat tcggtaaaat
     4801 caaaaatggt caaaacgtga cgctagaaga tgggactgaa attatcgcga gtgactatat
     4861 ttctcctcct cggcctggta aggtagtgac cattcttggt gatacgcgca agtgtcatgc
     4921 tagtgtccgt ctggctgtca atgctgatgt gctggtgcat gaggcgactt acggaaaagg
     4981 cgatgagaag attgctcgca agcatggcca ttcgaccaat atggaagccg ctcaggttgc
     5041 caaggatgct ggtgtcaagc agctcttgct taatcatatc agtccacgat ttttatccaa
     5101 ggatattagt caattacgga aagatgcaag tactattttc gagcaggttc atattgtcaa
     5161 ggatttagag gaaattgagc tatgagaacc attctaatca caggtgcctc tggtggcttg
     5221 gtacaggaaa tggtgccatt gctgaaagat gattttttga ttttactagg tcgtgatgtg
     5281 gcaaaaattg agcagctcta tgttcatcat gaaaaaaagg ctgtctttga tgtggacatc
     5341 cgagatgaaa tggccctgac ggcatttttt gatgaactgg atagtcagtt tggtcagatt
     5401 gatattttag tcaataatgc tggctatgcc atttatgatg attttgagaa tttttccagc
     5461 cagcaggtgc aggatatgtt tgacatcaat acttttgctc tgatgaccat gtgccgttta
     5521 gtaggtaaac gaatgaaggt aagacggagc gggcagatta tcaatatcat ttccatgtca
     5581 ggcttgatcg cttctgccaa gtcttcggtc tattcggcga ccaagtttgc agctatgggt
     5641 ttttcaaata ccattcgctt ggaattagcc cagtatggtg tgacagttac aactgttaac
     5701 ccaggaccta ttgcgactgg ctttttcggt caggcagatc ctgacggaag ctatcaagag
     5761 agcgtcaagg ctttcttgct gcaaccagac tatgttgcca agaagattgt ttcggctatg
     5821 gggacgaaaa aacgagacat caatttgcct tggtcactag cagctgctca taaactctat
     5881 actctctttc cgagaatagc agactacttg gccagtacag tatttaattt gaaatagaaa
     5941 aacgccgaaa taaaactcgg cgttttcgtg tagactagct acgctctttt ctacgtaagg
     6001 cgatactgcc tagaagtagg ctacctaatg cgatagcgat atagtttgtt ttttcacctg
     6061 tttgaggtag ttgttttgcc tcggtagact tggtggtatg tactactgta gtattttttg
     6121 gtgcttcatc caaaatattt tttggcattt caggttgtgg tacaagaatt tctacagctg
     6181 gtaagtcttc taaggttgct aattgactac caaatagggc gttggacacc caacggaaga
     6241 agtgaacagg gtggaccttg tttgttggat tacctgcata gataaagaca ggttggtctt
     6301 gataatttcc agcgatggcg acaattttat tggctaattt gtcatgacca ggccaccaac
     6361 ctgcgatata ataatcttta tcagcgattt taacaagcgt tttaaatcct tcaggaatac
     6421 cttcaatcca attaccggag ttggaataga aaagtccatt catggcgtag ccacttgtta
     6481 atggatcttt atcatcaata atagcccgca tgagaccttc atagtcacca ccatctgtaa
     6541 attgttccgc attgaaacct gctaagattc ctaattcctc cagtttttgc atggcgacac
     6601 cacctacgat gataattggt ttcttaccaa atacactggc atcgaattgg tctgattcga
     6661 gaatgatagc atcagcctca tctgcgctat tgacaatttc aaagcccatt cgttctacag
     6721 caagtgcagc atttgcaaga atggcaaagt cgcctgccca actataagag tgagaaggag
     6781 catacacctt cattgctttc aattcttgtc caataggctt cttatagaga ggctcgccgt
     6841 atagagcata ggtgtccagt aagtgactaa attggttagt ttccaagatg tagccatcgt
     6901 ctgtcaggta gaccttagcg cctgatttga tggcttgatt aatggcctgg acagcgcttt
     6961 cagaggtgtt tgtgacgaca taataaggcg ctttgaaatc aatttccgta gtacgtggag
     7021 cttttgtcca tgtgatgcta cctaatttgc tgctaaaggc attttcttca aatacagcct
     7081 tggctctaaa gcctttcata tctgggaagt tgaccaccaa ttcagcatac atggctcccc
     7141 aggcagattc atcagaacca gcgtagagga catggttagc gaatcctcgt ttggcttgag
     7201 ccatatcgac aactaggtcg cccttgcgga agtttccaac atcttcagtt aattccttga
     7261 caacaacccc attacgattg aaatattcaa tcatcttaaa tgcttcttgc atatcgttgt
     7321 gtttatcgag cgtcatagga atcacatagt agtctgggaa gaattttggc tggtcttttt
     7381 tgacacgtcc gacaacagct ccgtctggtc cgactagttc agactctgct tttggatctt
     7441 ctaccttatt gacaccgcgt gaatagtatt tgagacgcat ttccataaga ctatctggct
     7501 tggttgccat attgtgaaca ccgcccaaaa cagcaaagaa tcccgccttg aatgattctt
     7561 gatttccttc tggaatttca atcgtgtgtc caagaatacc gtggtacatt gcatacaccg
     7621 ctgtatagcc tgagaaggaa tcgtcccaac catctcccca gtggacgtta gggatggtgt
     7681 agcgttcata tttagaattt gcaatacctg ctcggcccat ttcatgagct ttttccagca
     7741 taatatcggc tagtaaatca tattcaaagt tcgggtcgtg tggcggagta gcaggctcga
     7801 tgagaaatcc agatacaaaa ccatgaatat ctaaaacaga tattggattc catttgtgga
     7861 tttgacggac gatggcttgt gtttctggat tcacttggaa accagtatca cggttagggt
     7921 ccaaaccatt tactagtgaa cgcaagtttt taacatcgcc atcaggattt tctgtgaaat
     7981 tgaacaagaa gataaattta tctaacaaat ctgtcacttt taaatgaaga gtaacaggat
     8041 ttccatcggc atctgtagat gggaaggtga tggtatcttt ttgtgcaaat tctttgaata
     8101 ggctagttac cacatctatg ccgggttgtt catcagcatg agtgttgttg atgagaatag
     8161 gtaatttata gtcgaaggtt ccagcttgca actgtttcag catctggtcg ggttgggtca
     8221 gcatgagtgg tgttgtttca gtaagatatt tatcaatact tgcttgatct ttagctacaa
     8281 cagccatttt aatgtctcta ccttcagctg attgaccaat tgactcaatc cttactaggc
     8341 ggtcggtcgc agcattattt ttcgtttcct caatctctgt caacatttcc tcatgtgtac
     8401 ggtaggcttc ataaggacgg aaatgaagag tttttgaaat tatcaagttt ccatcggcac
     8461 tagtaccttc caacagcata tcttttataa aatgtcgata ggtacggcga atattactcg
     8521 gtgtccgttt actcaaatct tctccaaaga gagctgcgag ctggatattc aaatgaagtt
     8581 ggttatcctg tctgctttct tctactgtga taaatgtatc gcctgtgaaa tctccctctt
     8641 tcaaattcca ggttttccat tcggaaatag gctttccatc caatgtccat tctatttttt
     8701 cagtcgtatc tggaagtgtg acggattctg tgatagtgac tgcttccgat agatagacag
     8761 tttttgtttc gagtgtaact gtatcaatag tcggtacggc agatttttct tctgtagtat
     8821 cctgattatc attattagct gatctgagcg tagtttcctc ggcattgggc tcggaagatg
     8881 ttgtctcttc ttctgtggaa acaagtgcag gagaagtatc tgctatttct tcagtttgtt
     8941 tttctgtctc cttcccttct acaagattat ctgtagcttc gcttgtgcta gttttcgtta
     9001 taggagtttc ttcaggcagt ggagtcactg tttcactagt agtaggtggt atctcctctg
     9061 tatttgcgaa gaccgttccc tgagcgagag atgtagcaaa aagaactgtt gaggcaagta
     9121 ggacttgatt caacagcctt ttcttatatg aaagtttcat gcgaacttcc tccttttgat
     9181 aatgtgtacg gggtaataaa ttaaaacggg ttatttcatg cttatcttat caaaaaatat
     9241 atgtctttac aatataaatt gaatatgttt atatatttaa aatattcaga aatatcgaat
     9301 catagagact atttattttg agtaggataa tgaa
//
LOCUS       DBNFEG010000077         9254 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.077, whole genome shotgun sequence.
ACCESSION   DBNFEG010000077 DBNFEG010000000
VERSION     DBNFEG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9254
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.077"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(134..922)
                     /gene="yghU"
                     /locus_tag="AC1PNG_001224"
     CDS             complement(134..922)
                     /gene="yghU"
                     /locus_tag="AC1PNG_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HIP6701731.1"
                     /translation="MTEYTKPLVWKWEDENDNRSGNRPTAGSRFEQALPKGEKSFQVY
                     SLGTPNGIKVAIMLEELKELGISEADYDLFLINIGQGDQFGSDFVAINPNSKIPAMVD
                     YSEQEPISVFESANILLCLAEKFDAFLPKTWAERTEVLNWLFWQTGAAPFVGGGFGHF
                     FHYAPTAQEYPINRYTMEAKRQLDLLDQLLATREYIAGNTYTIADIAIWSWYGRLVQG
                     QLYPGSAEFLDVDSYKHLKTWVEKIAQRPAVQRGLTVKYHSIDN"
     gene            complement(931..1221)
                     /locus_tag="AC1PNG_001225"
     CDS             complement(931..1221)
                     /locus_tag="AC1PNG_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701732.1"
                     /translation="MKVAEKEELYKYLSAAYNLPQEAFSEALREKILEVAGQLDKEEN
                     LYILAGHLSRFINAELTALTCRAPKELVQLAHYLQEVQNHYRYASLFPGKVK"
     gene            complement(1327..2016)
                     /gene="rplA"
                     /locus_tag="AC1PNG_001226"
     CDS             complement(1327..2016)
                     /gene="rplA"
                     /locus_tag="AC1PNG_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIP6701733.1"
                     /translation="MAKKSKNLRAALEKIDSTKLYSVEEAVALAKETNFAKFDASVEV
                     AYNLNIDVRKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINGGWLDFDVVIATPDMMAVVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGIVQALIGKVSFDADKLVENFKAFHDVMAKAKPATAKGTYMTSVSITTTQ
                     GVGIKVDPNSL"
     gene            complement(2057..2482)
                     /gene="rplK"
                     /locus_tag="AC1PNG_001227"
     CDS             complement(2057..2482)
                     /gene="rplK"
                     /locus_tag="AC1PNG_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIP6701734.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFITKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAASLEAAMRMIEGTARSMGFTVTD"
     gene            2637..3005
                     /locus_tag="AC1PNG_001228"
     CDS             2637..3005
                     /locus_tag="AC1PNG_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HIP6701735.1"
                     /translation="MFESIIFLKIVAFLFIFLTLAISIIAVKFFRLQNRGWNFADIAF
                     PLYAIEFYLISDKAYYNSLLPQLVLALSLLAIGLCGFFLLKKKNFLYRRFFKVFWRAS
                     FILTFLMYLALVIAVFTLKS"
     gene            complement(3368..3754)
                     /gene="gloA2"
                     /locus_tag="AC1PNG_001229"
     CDS             complement(3368..3754)
                     /gene="gloA2"
                     /locus_tag="AC1PNG_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMU1112c/YaeR family gloxylase I-like
                     metalloprotein"
                     /protein_id="HIP6701736.1"
                     /translation="MKFDAVHHIAIIGSDYDKTREFYVEKLGFEQVDEHIRPEKNDIL
                     FNVKKGNLILEIFIKPDAPMRPAMPNPEHTGLRHLAFQVADVEACLEEFDRLDIRHEV
                     LRTDDFDGKKMAFFFDPDGLPLEIHE"
     gene            complement(3754..6123)
                     /locus_tag="AC1PNG_001230"
     CDS             complement(3754..6123)
                     /locus_tag="AC1PNG_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIP6701737.1"
                     /translation="MRKTSKKGKATRRPTKAELAQQERVKNITLRVLGLLFIAFAASR
                     LGVFGVTSYNIFRLLFGSLAYLLLAGAFIYLLIPKVLREREGTISGFWLIVIGLLIEF
                     QAYLDWTYQGSDLFGHTLKLALSDLAKFQVTAFLGGGMIGSIFYLPVSFLFANVGSFF
                     IGLLVIAFGIFFVSPWSVYDVADGLAVAKDKLVETQVKRAELRTQKKAEREEKRQQEL
                     ARLEEERLRLEEEEESARALALVDRHVDLETGEILEEEPVQVPIISEYDHLDIEDEFP
                     IMLAEDDQPAQSNSASQTSEPNEFEKDEIDVQIDFKPKQRLAYKLPSIDLFAPIKAKS
                     QSNEKRIVRQNIKVLEDTFASFGIKVVVERAEIGPSVTKYEVKPAVGVRVNRISNLAD
                     DLALALAAKDVRIEAPIPGKSLVGIEVPNSEVATVPFRELWEQSKTDPDKLLEIPLGK
                     AVNGSVRSFNLARMPHLLVAGSTGSGKSVAVNGIISSILMKAGPDQVKFMMIDPKMVE
                     LSVYNDIPHLLIPVVTNPRKAARALQKVVDEMEKRYELFSQIGVRNLEGYNAKVEEFN
                     SRSEEKQIPLPLIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGIHMILATQRPSV
                     DVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKLLGRGDMLFKPIDENHPVRLQG
                     SFISDDDVEAIVGFIKDQADADYDESFDPGEVAEGDGDTGFGDTGGDPLFNEARALVV
                     ETQKASASMIQRRLSVGFNRATRLMEELEAAGVIGPAEGTKPRKVLETQ"
     gene            complement(6249..7607)
                     /locus_tag="AC1PNG_001231"
     CDS             complement(6249..7607)
                     /locus_tag="AC1PNG_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HIP6701738.1"
                     /translation="MKTVVEANQFIQTEKGNVNPIFKPQAHLTPETGWINDPNGFIYF
                     RGEYHLFYQFNPYESVWGPMHWGHAKSKDLVNWEQLPVALAPDKAYDKDGCFSGSAIV
                     KDDVLWLMYTGHINNEDGTVSQVQNMAFSTDGIHFEKIEQNPVATADGLPEEVIANDF
                     RDPKIFEKDGHYYSVVATKHKDNVGCIVLLSSPNLTDWKFESIFLKGEANQGFVWECP
                     DYFEVDGQEYLIISPMRYQKDGNDFININSNIFVMGHVDWDKKVFVAESFKEIDHGHD
                     FYAAQTTEGPEGERVMIAWMHTWGRPLVTNDLDHKWYGQMTLPRILKKTENGLHQVLP
                     TSVFEAFKDIEIGQSIQGPSKLSLRLEDSLELKLGNDQDYLQFGYDKAKQEVYINRSS
                     LKIQQVGEEEWSTVRRAVTVQATELEVLVDTNCLEIFINDGQETLTSTFYVEGQIILN
                     AL"
     gene            complement(7625..9235)
                     /locus_tag="AC1PNG_001232"
     CDS             complement(7625..9235)
                     /locus_tag="AC1PNG_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP6701739.1"
                     /translation="MKHKFGKTVVTLLASTVLLAACGSKNTASSPDYELKDVQFPLEK
                     SVTLKFMTASSPLAPADPNDKLIFQRLEEETGVHIDWTNYQSDFGEKRNLDIASGDLP
                     DAIHNDGASDVELMNWAKQGVIVPVEDLIDKHMPNLKKILDENPEYRSMITAPDGHIY
                     SFPWIEELGEGKESIHTVNDIAWINKEWLNKLGLEMPKTTDDLIKVLEAFKTQDPNGN
                     GKADEIPMSFVNGNGGEDFKILFGAFGVGDNDDHIVVNNDGTVDFTADNEDYKEGVEF
                     MRTLQEKGLIDPEAFEQDWNTYLAKGSENLYGVYFTWDKANITGMNETYDILPVLAGP
                     DGTKHITRTNGMGFQRDRMVITSANKNLELTAKWIDAQYKPIQSVQNNWGTYGDETQQ
                     NIFEYDAATDSLKHLPLNGTAPGELRQKTEVGGPLAILDEYYGTVTTMPDDAKWRLDL
                     MHATYLDYVTNNDIYPRVFMEQEDLDKIAQVEADMNDFIYRKRAEWIVNGGIEEEWDS
                     YLKELESYGLSDWLAIKQKYYDQYKENQ"
BASE COUNT         2476 a         2044 c         1856 g         2878 t
ORIGIN      
        1 tctaattctg attttcattg agtatcaatt attaatttct atttttggat tttcacaaag
       61 tctaactctt tcattataaa cttttatcat atcaaagtaa aagaaaaacc cacccataag
      121 gtgggcaagt caattaatta tcaattgaat gatatttcac tgtcaaccct cgttgtacag
      181 ctgggcgctg agctattttc tctacccagg tctttagatg cttgtaagaa tcaacatcta
      241 aaaattctgc tgaacctgga tatagttgac cttgcaccaa gcgaccatac caagaccaaa
      301 tagcaatatc tgcgatggta taggtatttc ctgctatata ttctcttgtc gccaatagct
      361 gatccaagag atctagctga cgcttagctt ccattgtata gcgattaatc ggatattctt
      421 gcgctgtcgg agcgtaatgg aagaaatgtc caaatcctcc accaacaaat ggagcggcgc
      481 cagtttgcca gaagagccaa ttaagaacct ctgtccgctc tgcccaagtt tttggtaaaa
      541 aggcgtcaaa cttttctgcc aggcaaagta gaatattggc tgattcaaag accgaaatcg
      601 gttcttgctc ggaataatct accatagctg gtattttgga gttggggttg atggcaacaa
      661 aatcggagcc aaattggtct ccttgaccga tattgatgag aaagaggtca taatcagctt
      721 ccgaaatacc taactctttc aactcctcca acataatagc aaccttaatg ccattcggcg
      781 tccccaatga atagacttgg aaagacttct ctccttttgg taaagcttgc tcaaaacgac
      841 tacctgctgt tggacggttg ccagatcggt tgtcattttc atcttcccac ttccaaacga
      901 gtggttttgt atattctgtc atgttttctc ctattttacc tttcctggaa aaagactggc
      961 atagcgataa tggttttgaa cctcttgaag gtaatgggct agttgcacca attccttagg
     1021 cgctcgacaa gtcagagctg ttaactctgc attgataaag cgacttaagt gtcctgccag
     1081 tatatagaga ttttcctcct tgtccagctg acctgccacc tccaatattt tttcacgcag
     1141 ggcttctgaa aatgcttcct gcggaaggtt gtaggctgca gaaaggtatt tataaagctc
     1201 ttctttctct gctactttca tcggataact ccttttatgt aaattgaata tagtataaat
     1261 cgaagtcaag tgcttgtcaa gaaaatctca aaaaaaaaga ttgccctaag acaatctttc
     1321 tattgattaa agtgagttag gatcaacttt gataccaaca ccttgtgttg ttgtaataga
     1381 tactgaagtc atgtaagtac ctttagctgt agctggttta gctttagcca ttacatcgtg
     1441 gaaagctttg aagttttcaa cgagtttgtc agcgtcaaat gatactttac cgataagagc
     1501 ttgtacgata cctgctttgt cagcacggta agtgatttta ccaccttttg actcttcaac
     1561 tgctttagca acatccattg ttactgtacc agttttaggg tttggcatca agttacgtgg
     1621 accaagtaca cgaccaagac gtccaacaac agccatcata tctggagttg cgataacaac
     1681 gtcgaagtcc aaccaaccac cgttgatttt agcaacgagg tcatcttcac caacaaagtc
     1741 tgcaccagca gcttttgctt cttctgcttt agcaccacgt gcgaaaacaa gaacacgtga
     1801 agttttacca gtaccgtttg gcaataccat tgcaccacgg atttgttggt ctgctttacg
     1861 tacgtcgatg ttcaagttgt aagcaacttc aactgacgca tcaaatttag cgaagttagt
     1921 ttcttttgca agagcaacag cttcttctac gctgtaaagt tttgtgctgt caattttttc
     1981 aagagcagca cgcaagtttt tgcttttttt agccatgtta tatgtctcct tgtaatcaga
     2041 taatcttggg tgctacttag tcagtaacag tgaatcccat agaacgagca gtaccttcga
     2101 tcatacgcat tgcagcttca agagatgcag cgttcaagtc aggcatttta gtttcagcaa
     2161 tttcttgtac ttgtgcacga gtaactgttg caactttagt tttgtttggt gtacctgaac
     2221 ctttttcaac acctgcagct tttttcaaaa gaacagcagc tggtggtgtt ttagtgatga
     2281 aagtgaatga tttatcttca tacacagaga taactactgg gatgatcata ccagcttggt
     2341 cagctgtacg agcgttaaac tcttttgtga atcccatgat gttgatacct gcttgaccaa
     2401 gtgctggtcc gactggtgga gctggagtag ctttacctgc aggaatttga agttttacga
     2461 gtttttcgac ttttttagcc atgatataaa atcctcctat tgtggttttg gcggtaatta
     2521 aagattttta cctcccacaa atatgtgttt tacacatacc ttttcattat acacgaattt
     2581 caaagaaatg caagctttag gtttactttt tttcacattt tttatagaat gaatgtatgt
     2641 ttgaatcgat tatattttta aaaatagtag cctttctatt catctttcta accttggcca
     2701 tttcgataat tgctgtcaag ttttttaggt tgcaaaaccg aggatggaac tttgcagaca
     2761 ttgcctttcc tctctatgca attgaattct atctgatttc ggacaaggcc tactataaca
     2821 gtctactgcc tcagcttgtc ctagccctct cactactagc gattggtctt tgcggtttct
     2881 tccttttaaa gaaaaagaat tttttatatc gccgtttctt caaggttttc tggcgagcta
     2941 gttttatctt gacctttttg atgtatctag ctttggttat tgcggtcttc actttaaaat
     3001 cctaaaatca tccatccttt catagatggc ataataaatc cccattgcag gcgcagtggg
     3061 gattcttttt tatactaaat cagacaaggc gacgtagatg tatagtcgaa tgaattagct
     3121 ttcagacaag gaactgaggt gcaggttgct agcacagcct agtggctgtg ctaggttgga
     3181 gatataactt gcaaagcaag tcacttctgc agagtacggc aagccgaaag tgacgatgta
     3241 tcaaagttaa ttcaaatgac tatagataga actgacgttc atcaaatcaa gtcaacagcg
     3301 tatgattttc taagagtatt aattagttgt catattgata ggcctctctg ggcatgaaaa
     3361 gatggactta ttcatgaatt tctagaggga ggccatctgg atcaaagaag aaggccattt
     3421 ttttaccatc aaaatcatct gttcgcagga cttcatgtcg gatgtccaaa cggtcaaact
     3481 cttccagaca agcttccaca tctgcaacct ggaaggctag atggcgtaga cctgtatgtt
     3541 caggattggg catggccgga cgcattggcg catctggttt gatgaagatt tccaagatca
     3601 gattgccttt tttgacattg aagagaatat catttttttc tgggcgaata tgctcatcca
     3661 cctgttcaaa gcctagtttc tcaacataaa attctcttgt tttgtcatag tcactaccga
     3721 taattgcaat atgatgaact gcatcaaatt tcattattgt gtttccaata cctttctagg
     3781 ttttgttcct tcagctggac caatgacacc cgctgcttct agctcttcca tgaggcgtgt
     3841 tgccctgtta aaaccaacag acaagcgtcg ttgaatcata gaggcgctgg ctttttgtgt
     3901 ctcaactacc aaggcacgtg cttcgttgaa aagtggatct cctccagtat caccaaaacc
     3961 tgtatcgcca tcaccttctg ccacttcacc aggatcaaag gattcatcat aatctgcatc
     4021 tgcttgatcc ttgataaagc caactattgc ttctacatca tcatcggata taaaggaacc
     4081 ttgcaagcgg actggatggt tctcatcaat cggtttaaag agcatgtcac cgcggcctaa
     4141 gagtttttca gcaccgtttt catctagaat cgtccgactg tctgtaccgc ttgatacggc
     4201 aaaggcgata cgagatggta cattggcttt aatcaaacct gaaataacat caacagatgg
     4261 tcgctgggta gcaagaatca tgtggatacc tgctgcacga gccttctgtc ccaagcgaat
     4321 aatggcatct tccacttcct tgctggctac catcatcaag tctgccaact catctacgat
     4381 taccacaatg agaggaagtg gaatctgttt ttcttctgaa cggctattaa attcttcgac
     4441 cttcgcatta taaccttcca agtttcggac accaatttgg ctaaacaact catagcgttt
     4501 ctccatttca tccactactt tttgaagagc tcgagcagcc ttgcgtgggt tggtcacaac
     4561 gggaatcaat aggtggggaa tatcattata gaccgataac tcgaccattt tggggtcaat
     4621 catcatgaac ttaacttggt ctggaccagc tttcatgaga attgaagaga tgataccgtt
     4681 gacagcaaca gacttccctg aaccagttga accagccacc aatagatggg gcatacgtgc
     4741 taggttaaag gaacggacgg aaccattgac agccttaccg agaggaattt ccaaaagctt
     4801 gtctgggtct gttttagatt gttcccagag ctcacggaaa ggtactgtag ccacttcgga
     4861 gtttggaact tcaataccga ccagagattt tcctgggata ggagcttcaa tacggacatc
     4921 tttagccgca agggctagag ccaaatcatc agctaggtta gaaatccgat tgacacggac
     4981 gccgactgct ggtttgactt catacttggt taccgatggt ccaatctcag cacgctcaac
     5041 tactaccttg attccaaaac tggcaaaggt gtcttctaaa actttaatat tctgacgaac
     5101 aattcgtttt tcattggact gactcttagc ttttatagga gcaaataagt ctatggacgg
     5161 aagtttatat gctagtctct gcttaggctt gaaatcaatc tgcacatcta tctcgtcctt
     5221 ctcaaactca tttggttcag aagtctggct tgccgagttt gattgtgctg gttggtcatc
     5281 ttctgccaac ataataggaa attcatcttc tatgtctaag tggtcatatt ctgaaataat
     5341 cggtacttga acaggctctt cttcaaggat ttcacccgtt tctaaatcga catgtctatc
     5401 taccagtgcc aaagcccgag ctgactcttc ctcttcctct agacgaaggc gttcctcttc
     5461 tagacgagct aattcttgct gacgtttttc ttctcgttca gccttcttct gtgttcgaag
     5521 ctctgctctt tttacctgcg tctccaccaa cttatctttg gcaaccgcca agccatcggc
     5581 aacatcataa acagaccatg ggcttacaaa aaagatacca aatgctatca ctagaagacc
     5641 gatgaagaag gaaccgacat ttgcaaagag gaaggagact ggcagataga agatacttcc
     5701 aatcatccct ccgcctagaa atgctgttac ctgaaactta gctaggtctg acaaggccaa
     5761 ttttagagta tgaccgaata agtcactccc ttgataggtc cagtctaagt aagcctgaaa
     5821 ttcaattaat aacccgataa caatcagcca gaagcccgat atggtccctt ctctttcgcg
     5881 taagactttg ggaataagaa ggtagataaa tgcacccgcc aataatagat aggccagact
     5941 accaaataat agccggaaaa tattgtagct ggtcactcca aacacaccta ggcgactggc
     6001 tgcaaaagca atgaataaaa gccccaatac tcgaagggtg atatttttaa cacgttcttg
     6061 ctgtgctaat tctgccttgg ttggtcgcct cgtagccttt ccttttttac ttgtctttct
     6121 catagatacc tcaactttct caatctcttt attctatcat aattgggaag aattttcatg
     6181 atagaaaaaa ccctgcaaat acagggtttc tcttggagtg aaagaataat ttaagagggc
     6241 aaataatatt atagagcgtt tagaataatc tgaccttcta cgtagaaggt tgatgtcaag
     6301 gtttcttgac catcattaat aaagatttcc aaacagttgg tgtcaaccaa aacctctagc
     6361 tctgttgctt gaaccgtgac cgctcgacga acagttgacc attcctcctc accaacttgc
     6421 tggattttga gagagctacg gttgatatag acttcttgtt tggctttatc atagccaaat
     6481 tgaagatagt cttggtcgtt tccaagtttt agttccaaac tgtcttccag tctaagagag
     6541 agcttgcttg gcccttggat actttgtcca atttcaatat ccttgaaggc ttcaaagaca
     6601 cttgttggca gaacttggtg caatccattt tcagtcttct tgagaatgcg tggaagggtc
     6661 atttgaccat accacttatg gtctaggtca ttggttacaa gcggacgtcc ccatgtatgc
     6721 atccaggcaa tcatgacacg ttccccttct ggaccttcag tcgtttgagc tgcatagaaa
     6781 tcatgcccat ggtcgatttc cttgaaggat tctgccacaa ataccttctt gtcccagtcc
     6841 acatgaccca tcacaaagat attggagttg atattgataa agtcattccc gtctttttgg
     6901 taacgcattg gagagataat caggtactct tgaccgtcca cttcaaagta gtcaggacat
     6961 tcccagacaa agccttggtt ggcctcacct ttcaagaaga tagactcaaa tttccaatcc
     7021 gtcaggttgg gagagctgag taagacgata cagccgacat tgtccttgtg cttggttgcg
     7081 acaacagagt agtagtgacc atctttctca aaaatcttcg gatcgcggaa gtcattggca
     7141 atcacttctt ctggcagtcc atctgctgtt gcgactggat tctgctcgat tttttcaaag
     7201 tgaataccgt cagttgagaa ggccatgttc tgaacttggc taactgtccc gtcttcattg
     7261 ttgatatgac cggtatacat gagccagagg acatcatctt tgacaatggc agagcccgag
     7321 aaacagccgt ctttatcgta agctttatca ggagctaggg caactggaag ctgttcccag
     7381 ttgaccaagt ccttactttt cgcatgcccc cagtgcatcg gtccccaaac gctttcgtaa
     7441 gggttgaatt ggtagaacaa atggtattct ccacggaagt agataaatcc attggggtcg
     7501 tttatccagc ctgtttctgg tgttaaatgg gcttgcggtt tgaaaatcgg attgacgttt
     7561 cctttttctg tctgaataaa ttgattggct tctactactg ttttcacagg cagcctcctt
     7621 ttgattactg attttcttta tactgatcgt agtatttttg cttgatagct aaccagtcag
     7681 aaagaccata gctttccaat tctttcaagt agctatccca ctcttcttcg ataccgccgt
     7741 ttacaatcca ttctgcacgt ttacggtaga tgaaatcgtt catatctgct tcaacttggg
     7801 caatcttgtc caaatcttct tgttccatga agacacgagg gtagatgtca ttgttggtca
     7861 catagtcaag ataggttgcg tgcatcaagt ccaaacgcca tttcgcatcg tctggcatgg
     7921 ttgttacagt accatagtat tcatcaagaa tagccagtgg tccaccaact tctgtcttct
     7981 gacgcaattc gcccggtgca gtaccgttca acggcaagtg tttcaagcta tctgtagctg
     8041 catcatattc gaagatgttt tgttgggttt catcaccata ggtaccccag ttgttttgaa
     8101 cagactggat tggtttgtat tgggcatcaa tccatttagc agtcaattcc aagtttttat
     8161 tagctgatgt gatgaccatg cggtcacgtt ggaagcccat accgtttgta cgagtgatat
     8221 gcttggttcc atcaggacct gccaatactg gcaagatgtc ataggtctca ttcataccag
     8281 tgatgttggc cttgtcccat gtgaagtaca caccatagag gttttcactt ccttttgcca
     8341 agtaggtatt ccagtcttgc tcaaaggctt ctgggtcaat gaggcctttt tcttgaaggg
     8401 tacgcatgaa ctcaacacct tctttgtaat cttcattgtc cgctgtaaag tcaacggtcc
     8461 cgtcattgtt tacgacgata tggtcatcgt tgtcaccgac accgaaggca ccaaagagaa
     8521 tcttgaagtc ttcgccgccg ttaccgttga caaaggacat tgggatttca tccgccttgc
     8581 catttccgtt tggatcttgg gtcttgaagg cttcaaggac cttaatcaag tcatccgttg
     8641 tttttggcat ttcaagaccg agtttgttca accattcttt gttgatccaa gcaatatcgt
     8701 taacagtgtg gattgattcc ttaccttcgc ccaattcttc aatccatggg aatgagtaga
     8761 tatggccgtc tggagccgta atcattgaac ggtattctgg attttcatcc aaaattttct
     8821 taaggtttgg catatgctta tcaatcaagt cttcaactgg tacgataacc ccttgttttg
     8881 cccagttcat caattccaca tcagaagccc cgtcgttatg gatggcatct ggcaaatcac
     8941 ctgaagcaat gtccaagttc cgcttttcac caaagtctga ctggtagttt gtccaatcga
     9001 tatgcacacc tgtttcttcc tcaagacgtt ggaaaatcaa cttatcattt gggtctgctg
     9061 gagctagggg ggagctagct gtcataaact taagcgtaac tgatttttca agcgggaatt
     9121 gaacatcttt caattcataa tctgggcttg aagcagtatt ttttgagcca caagcagcca
     9181 ataaaacggt acttgctaga agcgtaacaa ctgtttttcc aaatttgtgt ttcatcagaa
     9241 acaacctcct atat
//
LOCUS       DBNFEG010000078         9230 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.078, whole genome shotgun sequence.
ACCESSION   DBNFEG010000078 DBNFEG010000000
VERSION     DBNFEG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9230
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.078"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(38..2827)
                     /gene="ileS"
                     /locus_tag="AC1PNG_001233"
     CDS             complement(38..2827)
                     /gene="ileS"
                     /locus_tag="AC1PNG_001233"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIP6701740.1"
                     /translation="MKLKETLNLGKTAFPMRAGLPTREPEWQKAWDEANLYARRQELN
                     EGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFRAPFVPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLEMCRDYALSQVDKQRDDFKRLGVSADWENPYITLTKDYE
                     AAQIRVFGAMADKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GLLDTDSYIVVWTTTPFTVTASRGLTMGADIDYVLVQPAGSDRKYVLAEALVDSLAAK
                     FGWESFEVISKHKGAEFEYIVTEHPWDTEVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGVKYDLEVAVTVNERGLMNEAAGPDFEGQFYDKVVPTVKEKLGDLLLASEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDQIEATTFIPAWGKTRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVAALFEEHGSIIWWKSEAKDLLPAGF
                     THPGSPNGEFTKETDIMDVWFDSGSSWNGVMNARENLAYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAVLSQGFVLDGKGMKMSKSLGNTILPSDVEKQFGAEILRLWVTS
                     VDTSNDVRVSMDILGQVSETYRKIRNTLRFLIANTSDFNPASDAVAYEELRSVDQYLL
                     VKFNKLVAQIREAYDNYDFMGIYKSVVNFVTLDLSAFYLDFAKDVVYIDGAKSLSRRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEHEAEEFVQLAEMPEVETFANEAQLLA
                     DWENFMVFRTQAQKALEEARNAKVIGKSLEAHLTAYVSAETKSFLESLNADLAQLLIV
                     SNLTVTTEAAPESAVLVEEVAFTVERASGEVCDRCRRIDTSVAERSYGATICDHCASV
                     VEENFAEAVAQGFEA"
     gene            complement(2838..3281)
                     /locus_tag="AC1PNG_001234"
     CDS             complement(2838..3281)
                     /locus_tag="AC1PNG_001234"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6701741.1"
                     /translation="MIRLELVNKDNFEAVLQVQLAPEDQRRVASVEYSLAQAWLYKDS
                     GMILPYAVVSGRKVVGFAMLSIETKDNSYYLWRLLIDKDFQNRGCGKEVVQQMIGKAK
                     ADPLCHKISINYVIGNHKMRYILEKFGFQPVGLDGQKMKMELIIK"
     gene            complement(3512..4201)
                     /locus_tag="AC1PNG_001235"
     CDS             complement(3512..4201)
                     /locus_tag="AC1PNG_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIP6701742.1"
                     /translation="MALTALELKDKTFATKFRGYDADEVDDFLDIVTRDYEDLIRKNH
                     DQELELKNLRERLAYFDEMKESLSKSVLLAQDTAEKVKVAAEDQAANIIKQADYDAAT
                     LLHEAKDKANEILRNATDNAKKVVIETEELKNQTRIFHQRLKSTVESQLSLVNSSEWE
                     EILRPTASYIQTSDEAFRDVLHKALDEELPVEEESLDYTRQLTPEEIAELTRHAAAFE
                     SGESVVLSIEE"
     gene            complement(4210..5001)
                     /locus_tag="AC1PNG_001236"
     CDS             complement(4210..5001)
                     /locus_tag="AC1PNG_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HIP6701743.1"
                     /translation="MKNDKNLLQHFSREEREFVEKIMDMCQQVEDTYSYRLTHFLHPR
                     QDEIVCKIANYYQLQTFSSRDLVSTEHSRVIIAPTYYELDIKHFELTALHLSYARKFH
                     TLSHSQVLGTFLNQLGIKREYLGDILIDDERLIVFIDQKFGQIALQSITKVARVPVKG
                     KEEEWTTVRLPIGQEWRSKDVLVSSMRLDKLISVAFNLSRATANKLIEAGHVKLDYVL
                     TEQTSKLVEIGQLISVRRYGRVRLNEFLGFSKQGKIKLKLDIVKT"
     gene            complement(5004..5267)
                     /locus_tag="AC1PNG_001237"
     CDS             complement(5004..5267)
                     /locus_tag="AC1PNG_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIP6701744.1"
                     /translation="MQILIFLLLKIVEIYSYLLLAYALLSWFPSLYTSSIGRLIQWLV
                     APILKPFKRFNLQFMGLDWTVMAAMIALNMGTRFLVQLLLLLA"
     gene            complement(5272..5841)
                     /locus_tag="AC1PNG_001238"
     CDS             complement(5272..5841)
                     /locus_tag="AC1PNG_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HIP6701745.1"
                     /translation="MALKDTFKNLFNYFEVDEVNEVEEQADAYSMPNDRPKMRVANTT
                     VAPVREQQPKVETRREARSESQLQRLHERQQELMTNNNEKEIVKTTIDIKFPKRYEDA
                     PEMVNLLLDNASILIDFQYMSEQQARRCLDYLDGARSVLSGNLKKVSNTMWLLTPVNV
                     TVNIEELRNANAGTTTGAADSNFDFDIKR"
     gene            complement(5858..6529)
                     /locus_tag="AC1PNG_001239"
     CDS             complement(5858..6529)
                     /locus_tag="AC1PNG_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIP6701746.1"
                     /translation="MNFQKNKDAIFSAVAKAAEKAGRPSESVKVIAVTKYVDSSIANQ
                     LVNTGITHIGENRVDQFLEKYNALADKQLTWHLIGTLQRRKVKDIIPFVDYFHALDSV
                     KLAQEIDKRATKIVKCFLQVNVSGEESKHGFAVSEIDAALAEISLLENIELVGLMTMA
                     PIDATDDELDEIFSKIQEIQEELATRNLPRMPFTELSMGMSGDFERAIAHGATYVRIG
                     SAFFE"
     gene            complement(6536..7765)
                     /gene="ftsZ"
                     /locus_tag="AC1PNG_001240"
     CDS             complement(6536..7765)
                     /gene="ftsZ"
                     /locus_tag="AC1PNG_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIP6701747.1"
                     /translation="MAFSFEAAASHGAVIKVIGVGGGGGNAINRMIEEGVAGVEFIAA
                     NTDVQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEALTNVLTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKNLGALTVAVVTRPFGFEGNKRGNFAIEGIEGLREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MENKGNALMGIGIGTGEDRVIEAARKAIYSPLLETTIDGAEDVIVNVTGGYDMTLTEA
                     EDASEIVNQAAGQGVNIWLGTSIDETMKDEIRVTVVATGVRQDTADKPARHRMEAVSP
                     RPSQRFDHSVASAPTRGAAQQTEAPKASAFGEWDLRRENLIRPTDTEPTSTVSVEKFT
                     MDHDEDELDTPPFFRNR"
     gene            complement(7791..9170)
                     /gene="ftsA"
                     /locus_tag="AC1PNG_001241"
     CDS             complement(7791..9170)
                     /gene="ftsA"
                     /locus_tag="AC1PNG_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIP6701748.1"
                     /translation="MARNGFFTGLDIGTSSVKVLVAEYIDNEMNVIGVSNVKSAGVKD
                     GIIVNIEVAAGAIKKAIEQAEEKSGIRIEKVNVGLPANLLQIEPTQGMIPVTTDSQEI
                     TDLDVENVVKSALTKSMTPEREVISFIPEEFTVDGFQGIKDPRGMMGIRLEMRGMLYT
                     GPRTILHNLRKTVERAGVQVENIVISPLALTRSVLNEGEREFGATIIDLGGGQTTVAV
                     MRGQELQYTNIYQEGGDYITNDISKVLTTSKSIAENLKYNYGIAYPQDASDKEKFTVE
                     VIGENAPVEVTERYLSEVIAARLRQIFDRVKQDLERTRALDLPGGIVIVGGGAILPGI
                     TELAQEVFGVNTKLFVPNQIGIRNPAFASVISFVEYVGELEDVENIAQHAVNGETTLR
                     HKPVEIPVARPRISQPIQREIVNELNTVDQVEEQVAPIYHDEEEEVRPAAKGNITDRI
                     RGLFGSMFE"
BASE COUNT         2629 a         2141 c         1657 g         2803 t
ORIGIN      
        1 atcgctagat agactttaac aaatgtattg tgattgctta ggcttcaaaa ccttgagcta
       61 ctgcttcagc aaagttttct tccactacgc ttgcacagtg gtcacagatt gttgcgccat
      121 agctgcgctc tgcaacacta gtatcaatac ggcggcaacg gtcacatact tcaccgcttg
      181 cacgctctac agtaaaggca acttcttcta ccaaaacagc actttcagga gctgcttccg
      241 tggttactgt caagtttgaa acgatgagca actgagcaag gtctgcattc aagctctcca
      301 agaaggactt ggtttcagct gagacatagg cagtcaaatg agcttcaagt gacttaccaa
      361 tcactttggc attgcgtgct tcttccaagg ctttttgtgc ctgagtacgg aagaccataa
      421 agttttccca atcagctaaa agctgcgctt cattagcaaa ggtttccacc tctggcattt
      481 ccgccaactg aacaaattct tctgcttcgt gttccaagta agaccagatt tcttctgccg
      541 tatgtggtaa gattggcgtc aagagcttgg taattttcac caagatgtca tagaagacag
      601 tttgcatctg acgacgagaa agtgatttag caccatcaat gtagacgaca tctttggcaa
      661 aatcgaggta gaaggctgac aaatcaaggg ttacaaagtt tactacagat ttatagatgc
      721 ccatgaaatc ataattgtcg taggcttcac ggatttgagc taccaattta ttgaatttca
      781 ccaagagata ctggtcaaca gaacgaagct cttcgtatgc caccgcatca cttgcaggat
      841 tgaaatcaga agtgttggca attaagaagc gaagggtatt acggatttta cggtaggttt
      901 cagaaacctg tccaagaata tccatagaca cacggacatc gtttgaagta tcaacggatg
      961 tcacccacag acgcaagatt tctgcaccaa actgcttctc aacatcactt ggaaggatag
     1021 tatttcctag ggatttagac atcttcatac ctttaccgtc caatacaaag ccctgtgaca
     1081 agacagcctt gtatggcgca tgaccattta cagcaacaga ggtaatcaag gatgagttaa
     1141 accaaccacg gtattggtca gaaccttcta ggtagaggtc tgctgggtag gcaaggtttt
     1201 cacgggcgtt catgacacca ttccaagatg aaccagagtc aaaccatacg tccataatgt
     1261 ctgtttcttt ggtaaattcg ccatttggtg aacctggatg tgtgaaacca gctggcaaga
     1321 gatcttttgc ttctgatttc caccagatga tagaaccatg ctcttcaaaa agggcagcaa
     1381 cgtggtctgt cacttccttg gtcataatcg ctgttccatc ttctgcatag aagattggaa
     1441 gtggcacacc ccaagcacgc tgacgagaga tgacccagtc accacggtca cggatcatgt
     1501 tgtaaagacg tgttttaccc caagcaggaa taaaggtcgt tgcttcgatt tggtccaaga
     1561 tttcctgacg gaatttagaa acagaggcaa accactgtgg cactgcacgc cagatgattg
     1621 gcttcttggt tctccagtca aatgggtagg agtgattgat cacttcacta gcaaggagta
     1681 agtcgcctaa tttttcctta accgttggta caaccttgtc atagaattgc ccttcaaaat
     1741 caggacccgc agcttcattc atcaagccac gttcgttgac agttaccgca acctccaagt
     1801 catatttgac accgacattg tagtcatcct caccaaaacc tggagccgta tggacgatac
     1861 cagtacccga gtccgttgtg acatggtcac ccaagataac caattcatca acttcagtat
     1921 cccatgggtg ttcggtcacg atatattcaa attcagcacc cttgtgtttt gaaatcactt
     1981 cgaaggattc ccaaccgaat ttggctgcca agctatcaac caaggcttct gccaagacat
     2041 acttacggtc agagccagca ggttgaacca agacatagtc aatgtctgct cccattgtca
     2101 aaccgcgaga agccgttact gtaaatggag tggttgtcca gacaacgatg tagctgtctg
     2161 tatccaagag ccccttgcca tctttgacct tgttggcata atacagagat gttgagtcaa
     2221 tgtcatggta ttcgatttca gcttctgcaa gggcagattc agatgaccat gaccagtaga
     2281 caggcttagc accgcggtag atatagccct tgtctgccat agcaccgaag acacggatct
     2341 gagctgcttc gtagtctttt gtcaaagtga tatatggatt ttcccagtct gcagagacac
     2401 ccaagcgttt aaaatcatca cgttgcttgt caacttggct aagagcataa tcacgacaca
     2461 tttcaagata ttcaactagg tccatttcct tgcgtttaac accttgtttt gccaagactt
     2521 gctcaatagg aaggccgtgc gtatcccaac ctggtacaaa tggagcacgg aaaccagaca
     2581 ttgacttaga gcgaacgata atatctttag aaatcttgtt taaagcatgt ccaacgtgga
     2641 tatttccgtt agcgtatgga gggccatcgt ggaggtggaa ggcaggtttg ccttcattga
     2701 gttcttggcg acgagcatac aagtttgctt catcccaggc cttttgccat tctggctcac
     2761 gtgttggtaa gcctgcacgc ataggaaatg ctgtcttacc taaattgagt gtttctttta
     2821 gtttcatcat aactccttta ttttataatt aattccattt tcattttttg accatctaaa
     2881 ccgacaggct gaaagccaaa tttttctaag atgtaccgca ttttatgatt accaatgaca
     2941 taatttatag aaattttatg acaaagcgga tccgccttcg cctttccaat catttgctgg
     3001 acgacttcct ttccgcatcc acgattttga aaatccttat caatcaacaa gcgccataaa
     3061 taataactat tatctttggt ttcgatagat aacattgcaa aaccaacaac ttttctgcca
     3121 gataccacag catatggtag aatcatcccc gaatctttat ataaccaagc ttgggctaat
     3181 gaatattcaa ctgatgctac tcggcgttgg tcctcaggtg caagttgaac ttgtaacact
     3241 gcttcaaagt tatccttgtt tactaattct aatcgtatca taaaaataaa aaaactcccg
     3301 ccaaataagg acgaaagttc gtggtaccac cttagtttag atagagtttc ccctatccct
     3361 ctatgttcgt aacgtgaaca gacgtctctc cttactattt tcaggaaaaa tcaaacaaag
     3421 tgatgatcca tagaaagaga ttgtagggct tccaccatct cctactcgct taaaatattt
     3481 ctaacgacct tgtctttgct tatgatttct attattcttc tattgaaagt actacagatt
     3541 ctccactctc aaaagctgct gcatgacgag ttaattccgc aatctcttct ggtgtcaatt
     3601 gacgtgtata atccaaactt tcttcttcaa caggtaattc ttcatccaaa gccttatgaa
     3661 gaacatcacg aaaggcttcg tcacttgttt gaatgtaact tgctgttggg cggaggattt
     3721 cctcccattc agatgaatta accagtgata attggctttc tactgttgat ttcagacgct
     3781 gatggaaaat acgtgtctgg ttttttaatt cttcagtttc aatgacaact tttttcgcgt
     3841 tgtcagtcgc attacgaaga atttcatttg ccttatcttt agcttcatgt aacaatgttg
     3901 ccgcatcata gtcagcttgt ttaataatat ttgccgcttg atcttcggcc gcaaccttta
     3961 ctttctcagc tgtatcttga gctaaaagaa ctgatttact caatgattct ttcatctcat
     4021 caaaataagc caaacgttcc cgcaaatttt tcaattctaa ctcttgatca tgatttttac
     4081 gaattaaatc ctcataatca cgtgttacaa tgtccaaaaa gtcatcaact tcatctgcgt
     4141 cgtatcctct aaattttgta gcaaaggttt tatcttttaa ttctaatgct gtaagtgcca
     4201 tctttcctcc taagttttaa caatgtctaa ctttaatttt atcttccctt gcttagaaaa
     4261 acctaaaaat tcatttaggc gaactctacc atatctccta acactaatta attgccctat
     4321 ttcaactaat ttactggttt gttctgtcag aacataatcc aacttcacat gtccagcctc
     4381 aatcaacttg tttgcagttg cccttgaaag attaaatgcg accgaaatca atttatccaa
     4441 tctcatacta gaaaccaaca catcctttga acgccattcc tgtccaatcg gtagtcgaac
     4501 agtcgtccat tcttcttcct ttcccttaac agggacacga gcaactttag tgatagattg
     4561 taaggcaatc tgcccaaatt tctgatcgat aaagacaatc aatcgttcat catcaatcaa
     4621 aatatcaccc aaatactccc ttttaatccc tagttgattg agaaaagttc caagaacttg
     4681 tgaatgggat aaggtgtgaa attttctagc ataggacaaa tgaagagcgg ttaactcaaa
     4741 gtgcttgatg tccaactcat aataagtcgg ggcaataata acccgcgaat gttctgtcga
     4801 aacaaggtca cgactagaaa acgtttgtaa ctggtagtag ttagcaattt tgcatacaat
     4861 ttcatcttgt ctaggatgta aaaagtgagt taatctgtat gaatacgtat cttcaacttg
     4921 ctgacacata tccattattt tctctacaaa ctctctttct tccctagaaa aatgttgcaa
     4981 taagtttttg tcatttttca taactatgct aacagtagca ataattgaac caagaagcgt
     5041 gttcccatat taagcgcaat catggctgcc attacggtcc aatccaaccc cataaattgt
     5101 agattaaatc gcttaaaggg ttttaaaatc ggtgctacca gccattgaat caaacgacca
     5161 atcgagcttg tgtataggct aggaaaccaa cttagcaaag cgtatgccaa taagagataa
     5221 gaataaatct ctacaatttt taatagtaaa aaaatcaaaa tttgcatagc gttaccgttt
     5281 tatatcaaaa tcaaaatttg aatcagccgc accagtagtt gtaccagcat tagcattgcg
     5341 caattcttca atattgactg tgacatttac tggagttaat agccacatgg tatttgagac
     5401 cttttttaaa tttccagata atactgaacg agcaccatcc aagtagtcta ggcaacgtct
     5461 tgcttgttgc tccgacatat attgaaaatc aatgagaata ctcgcattgt ctaataaaag
     5521 atttaccatc tctggtgcat cttcataacg ttttggaaac ttaatatcaa ttgtagtttt
     5581 gacaatctct ttttcgttat tattagtcat caattcttgc tgcctttcat gtaggcgttg
     5641 aagttgactt tctgaacgtg cttcccgacg tgtctctact tttggctgct gttcgcgcac
     5701 aggtgcaact gtagtattgg caacacgcat tttaggacga tcattaggca ttgaataggc
     5761 atctgcctgt tcttcaactt cattgacttc gtcaacttca aagtaattaa ataagttctt
     5821 aaatgtatct tttaatgcca tattttcctc ctctccatta ttcaaagaat gctgagccaa
     5881 ttctcacata tgtagctccg tgagcaatgg cgcgttcaaa gtccccactc attcccatac
     5941 ttaactctgt aaagggcata cgtggtaagt tccttgtagc tagttcttct tggatttcct
     6001 gtattttaga aaaaatctcg tctaattcat catctgtagc atcaatcggt gccatcgtca
     6061 tcaatccaac aagttcaatg ttctccaata aactaatttc agcaagtgcc gcatcaatct
     6121 cagatactgc aaagccatgc ttactctcct caccggaaac gttgacttgg aggaaacact
     6181 tgactatttt agtcgcacgt ttatctattt cctgagctag tttgacagag tctagggcgt
     6241 ggaaataatc aacaaatgga ataatgtcct tgacctttct tctctgtaaa gtccctatca
     6301 aatgccatgt caattgtttg tctgctaggg cattatactt ttctaagaac tgatcgactc
     6361 gattttctcc aatatgagta attccagtat tcactagctg attagcaata gagctgtcaa
     6421 catatttggt gacagctatt acctttactg attcacttgg acgacctgcc ttctcagcag
     6481 cttttgcaac tgctgaaaag atagcatcct tattcttttg aaaattcatt ttagtttagc
     6541 gattacggaa gaatggtggt gtatccaatt catcctcatc atgatccatt gtaaattttt
     6601 cgactgaaac agtggatgtt ggttctgtgt cagttggacg aatcaagttt tcacgacgca
     6661 aatcccactc tccgaaggca gaagctttcg gcgcttcagt ttgttgtgca gctccacgag
     6721 ttggtgctga tgctactgaa tgatcaaagc gttgagaagg gcgtggtgaa acagcttcca
     6781 tgcgatggcg ggctggttta tcagcagtat cttgacgaac acccgtagcc acaacggtca
     6841 cacgaatctc atctttcatt gtttcatcaa tagatgtacc caaccaaatg ttaacacctt
     6901 gacccgcagc ctgattaacg atttctgaag catcttctgc ttctgtcaga gtcatgtcat
     6961 agccacctgt aacattgaca atgacatcct ctgcaccatc aattgttgtt tcaagaagag
     7021 gagaataaat cgctttacga gccgcctcaa tcactcggtc ctcaccagta ccgataccga
     7081 tacccatcaa ggcgtttcct ttgttttcca taactgtctt cacatctgca aagtccaagt
     7141 taatcaaacc aggatttgta atcaagtctg taattccttg aacaccttga cgaagaacat
     7201 tatcagcttc gcttagtgct tctaaaagcg gtgttttctt atcaacaatt tcaagaaggt
     7261 tgttgtttga aataattaaa agtgtatcta cttgttcacg aagaccttca attccctcaa
     7321 tcgcaaagtt tccacgttta tttccttcaa aaccaaacgg acgagtcaca actgcgacag
     7381 tcaacgcacc caagttttta gcaatacgtg caataacagg agccgcacca gtccctgagc
     7441 caccgcccat accagcagtg atgaagacca tatctgcacc agttaataca ttagtcaatg
     7501 cttcttcgct ttcctcagca gctttccgac caacctcagg ttgacctcca gcacccaaac
     7561 cacgagtcaa tttaggacct agttggataa ctgtttcagc tttagagcta cttagcgctt
     7621 gtacatctgt gtttgctgca ataaactcaa ccccagcaac accttcttca atcatacggt
     7681 taatcgcgtt cccgccacca ccgccgacac cgattacttt aatgacagca ccatgactag
     7741 ctgctgcttc aaatgaaaat gccatagttt aaatcacctt ttcttttctc ttattcaaac
     7801 atgctgccaa ataaaccacg aatgcgatct gtgatgtttc ccttagcagc tggacgaact
     7861 tcttcttcct catcatggta aataggagca acttgttcct ctacctgatc aactgtgttc
     7921 aattcgttca cgatttctct ttgaattggc tgactaatcc gaggacgagc aacaggaatt
     7981 tcaactggct tatgtctcaa ggtagtttca ccatttactg catgttgggc aatattttct
     8041 acatcttcta actcaccaac atactcaaca aagctaatca cacttgcaaa tgctggattg
     8101 cggataccaa tctgatttgg aacaaaaagc ttagtgttaa caccaaacac ttcttgggcc
     8161 aattctgtaa ttcctggtaa gatagcacca ccacctacaa taacaatgcc tcctggtaaa
     8221 tctagagcac gtgtacgctc caaatcttgt ttcacacggt caaaaatctg acgcagacgt
     8281 gccgcaatca cttctgaaag gtagcgctcg gtcacttcaa ctggagcgtt ttctccaatc
     8341 acttcgacag tgaatttttc cttatcactt gcatcttgcg gataggcaat gccgtagttg
     8401 tatttcaagt tttcagcaat actctttgaa gttgtcaata cttttgaaat atcgtttgtg
     8461 atataatctc caccttcttg gtagatgttt gtgtattgca actcttggcc acgcataaca
     8521 gccacagtcg tttgaccacc accaagatca atgatagtcg caccaaactc acgttcacct
     8581 tcattcaata cagagcgagt tagtgccaac ggagaaatta caatattttc aacttggaca
     8641 cctgcacgct ccaccgtttt acgaagattg tgaagaatgg tacgtggacc tgtatacagc
     8701 ataccacgca tttccaagcg aattcccatc attccacgag ggtccttaat gccttggaat
     8761 ccatcgacag tgaactcttc tggaataaat gaaatcactt cgcgttccgg agtcatactc
     8821 tttgtcaatg ctgattttac aacattctca acatccaagt ctgtaatttc ttgtgaatct
     8881 gttgtaacag gaatcatccc ttgagtcggt tcaatctgaa gaaggttggc aggtaatcca
     8941 acatttacct tttcaatgcg gatacctgac ttttcttctg cctgctcgat cgctttttta
     9001 atcgctccag cagccacctc aatatttaca ataattccat ctttgacgcc agcacttttt
     9061 acattgctga ccccaatcac attcatttcg ttatcaatat attctgcaac caagacttta
     9121 acggagcttg taccaatatc taatcccgta aaaaagccgt ttctagccat tctatcagtc
     9181 ctctcttact tccaacttta acaaatcatc ttccattttg aaaaatagta
//
LOCUS       DBNFEG010000079         9168 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.079, whole genome shotgun sequence.
ACCESSION   DBNFEG010000079 DBNFEG010000000
VERSION     DBNFEG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9168
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.079"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            289..2319
                     /locus_tag="AC1PNG_001242"
     CDS             289..2319
                     /locus_tag="AC1PNG_001242"
                     /inference="COORDINATES: protein motif:HMM:NF013727.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HIP6701749.1"
                     /translation="MLFFKNLKKGMKPSGVVDDQSWSNSRFKLALKVADRSDSMEMLK
                     TPDAAEITQVGRGYLQVGNNEVYELFQSAWSGADYQPDKDEQGIEDHTIYAINDIGQY
                     EILNEDLSGLEQAESIKEVPSELQAIVSKLKALTERLSIQALPQPWLPPLSKEIFLQD
                     LRPQGFKDLWGQASGLVARLGMVDIPSRQSQEVLEHDFEKDGHIALFSSPGMGKSTFV
                     QTVLVDLFRQNNPDRLHTYLLDFGTNGLLALNDYPHVAEYFLVEETEKLQKLIKRLQD
                     EIRERRKLFTDSSVHNIEMYRKLGDRKLADIVIAVDSYDGLKGSEVEVAIDTLLQTLA
                     RDGMGLGIYLILTASRTGALKNNLYSNFKRRIALKMNDDSEARAIVGRTALVIEDVPG
                     RGLIQVDDPELIQLAMPAKGENGLEMIEALRHESQEMAKNWNGALPQGIVIVPEVLDE
                     QTFLQMVETEKAIASDFIPIGLEFEEVHTISLPIPKLKHLLILTEKEQHKLVAVRHLL
                     NMLWHVRKGQSVTLFDVTDEYGRFAGKVKQYYSSSELTENVVEELKMRVLHARKQKEQ
                     LNEFVIISDLPAFMTVDGMSDNDFALLYEEGQRVGLHLIVVANKTYMSLSSGIQRLIK
                     QKLDTVLIAMKMSNQSVVARSEVGREAELAIDEVYLHYQDQQIKLKITKEIE"
     gene            2330..2785
                     /locus_tag="AC1PNG_001243"
     CDS             2330..2785
                     /locus_tag="AC1PNG_001243"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701750.1"
                     /translation="MQKEKMTVEEFLQLKKDAQVSLILFIVFGLVLFSSMFYITVIGA
                     RTQMYNSLVTMVFLSVLMSAVRPYVFSKNILEKKQPELKQYSTEGYSVSSAFLKRVFL
                     VYSVAIIVVVFGFASAYATRVETILPDDTLPSLEQKSIIEQKLEGNSNE"
     gene            2859..3164
                     /locus_tag="AC1PNG_001244"
     CDS             2859..3164
                     /locus_tag="AC1PNG_001244"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701751.1"
                     /translation="MSHNLHAQRSLSGLQSYIEHCQKVIDRIDSQDGYGYLANFRDKL
                     ADDIKVLKDYRNFVKDSNASFVDLYQTLNAKIGNLNASIANYKSMYNDGKPVWEWVW"
     gene            3173..3505
                     /locus_tag="AC1PNG_001245"
     CDS             3173..3505
                     /locus_tag="AC1PNG_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701752.1"
                     /translation="MDTNINSIDSQYTYDTIVYDDGQIDGVIQQLNQIIDCLNQISSE
                     IVGLEAQDSSWSGKSKAAYVDLKEFLKSYRKDYQHSIKELKSTVSGLETLLNSIPSAT
                     VLKEIDGA"
     gene            3502..4158
                     /locus_tag="AC1PNG_001246"
     CDS             3502..4158
                     /locus_tag="AC1PNG_001246"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701753.1"
                     /translation="MIFSIEALYTLHLLNDHSDDLSFLPLPNGQNLDRKSLLKETLEK
                     GFEDLKELQLVVDNQLTERGVEYGYYLEEYHSSPYHCRVDLSYFCAPAVDEFKRYSIL
                     IKEVDEGVFVIERLASVWFLGFLLETHPILHHLDDKMKDYLMYDWTPKSHFSLLVEHG
                     RSEGLRLAVEQLGTIYSDTLFLEIEESIYEYDLTRELMRSIDSETLQSRLIKDLKVEV
                     "
     gene            4161..4595
                     /locus_tag="AC1PNG_001247"
     CDS             4161..4595
                     /locus_tag="AC1PNG_001247"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701754.1"
                     /translation="MSEQIQISLSSQEQIILHALRITELATDITQTIQQVVETIPNFS
                     SQGSFHTIYTTGKNDGFYRYVLKAQELKTLSEVLYRHVETTHQKMVDMDRALAVHITN
                     QFLNSPSTSSEDKQFIREHPEEAVKYIQSEMKKSAPSSGGGS"
     gene            4592..6661
                     /locus_tag="AC1PNG_001248"
     CDS             4592..6661
                     /locus_tag="AC1PNG_001248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701755.1"
                     /translation="MTRFYTNRSDFEQNHWLDSQKSRVVAMLRDIDSVSSTVQSEGLS
                     HLYRSRGEASLGASKPSSTFSQAVDRVDSMLRSAGKIHKDIVQNIDSKFTKGLDKSFQ
                     SLNEVNGSKNPYMSKGKYEKTYTTTNYTSNYGYMTQTHTRQVHYSLSDILDGTKSPIP
                     AAQSLYQDRVKNLREQLRQTDQLTDKQIKELESKSDAELVQEFYPTLESDYSQIKNST
                     WQEENKEALGLVDTGLKILAGIALAAGTVMTGGAALPLALAGGAYLFADGAHSAITGN
                     TLITGTQLSTEERIWAGAEAVVTLATFGSFVAGQGALRAGKQIPTTVQTIGKLAGYAD
                     EGVELGQLASGILSGDGTNATLNYIGGKAFEGIVESSVKGLQSQLSHIEVSSPSQIQL
                     NARQGQNFVDLQTVEFGKIASKVENNITIKMTVDGVEQKIRVDAIGFDEAGNIRIQEY
                     TTAQNGLKISRQNLLEDLSKYGGTIVGAGKGDFVGGVEIPKGTRIDVVSQKTGNVTIG
                     SLDTVYSVKDFKAEMEQLPHINLSKKVEDGLVSTNNGSRTSPHHVYSVEEIATHLAMF
                     DDGAVKIMSFESYDYGLQNYGATIGHPDGHYVMPKFVYEKAVRASNGNPRVLEGLLGL
                     EQGYLGNNPVVIDIQRLKNLRMPSGNEPGAWQGLWEPGGYTQGGIPEAVTDQLSESDY
                     TIDEVFK"
     gene            6677..7537
                     /locus_tag="AC1PNG_001249"
     CDS             6677..7537
                     /locus_tag="AC1PNG_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6572 domain-containing protein"
                     /protein_id="HIP6701756.1"
                     /translation="MKVLRGTDFIIYELNGQYSINWYRGRERFSYPIDEDLVKKALLT
                     SKDGKEVMFYVEHGRWPEEDELEHYNESNVITHRGNNFIVYEENGKYEMHFMTGGFQD
                     REVIYPISKELMERAFESPQDAYEVKIYLMTGHWPDKSQEELDRNFLRQFPEQILKNP
                     TIPKTLFSEYEFTKLLKQAIETVLQTSKIDSLGIVEEHLELLLLDPIKWQEETEIIHL
                     QLLQEKLNNYIHFIESKQYVESYGDDFTEKVINLTFQYAPSDNGLAFLMQVQKVLQPT
                     DIRLKVVVPE"
     gene            7863..8480
                     /locus_tag="AC1PNG_001250"
     CDS             7863..8480
                     /locus_tag="AC1PNG_001250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701757.1"
                     /translation="MDAIGFDEAGNIRIQEYTTAQNGLKISRQNLLEDLSKYGGTIVG
                     AGKGDFVGGVEIPKGTRIDVVSQKTGNFSIDSTPNYIQVGRYTTELSKIDLPLEEKVI
                     RLQEFYSDLSDKTDINVPSDPQYVVAVRDGWVEYDWPKNLGYQEGTVQSITRDSGLPD
                     QWDRFGHMGGGNFSDIPSDGPYTYSQRAIPYVENPNAYHKGTFIR"
     gene            8477..8758
                     /locus_tag="AC1PNG_001251"
     CDS             8477..8758
                     /locus_tag="AC1PNG_001251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701758.1"
                     /translation="MSYTDFIKLYQESLKVGVQLIIGAQKSSLLKTDLSIKYIKENLV
                     TAIVAQRLYDQSIVQHKMTSREETLKVDEVYLYHDQDYQKVKISKQVVE"
     gene            8758..>9168
                     /locus_tag="AC1PNG_001252"
     CDS             8758..>9168
                     /locus_tag="AC1PNG_001252"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701759.1"
                     /translation="MNRIKKLMMCILGGVTIMGLTGCNKEVEIRTELAPMYEVLDQQS
                     IENFDIFSIEDNLRIYGMESAKAFKTDLAISPDGQFEGMSYDLSVSETEGYPTTYIDG
                     NLKINTTSEVLSDRKMMFEEFHFSEDYFNSLELVK"
BASE COUNT         2797 a         1578 c         2230 g         2563 t
ORIGIN      
        1 ccgttggttg gaaccttaat ttttgtcatg aataaaaatg aacagccaaa ttatttggaa
       61 tatggcaata aatatcttaa atagtcaaac ttatatatta ccaatcatag attatttcta
      121 tccagacaag attaccttgt ctggattttt tggtataatg gaaaagtaga aatattcttc
      181 atttgtgcat aaacggttag gctaggcttg gtctaaagcg gagctggagg atgagatgga
      241 agtagaatcg ctttatttta tttctatttc gaaacgatgt gagcagctat gctatttttc
      301 aaaaacctca agaaaggtat gaagccatct ggtgtggtgg atgaccagag ctggtccaac
      361 tcccgcttca agctggcgct caaggtggca gatcggagcg actccatgga gatgcttaag
      421 acgccagatg cagctgagat tacccaggtg ggtcgtggct acctccaagt cggaaacaat
      481 gaagtgtatg aactcttcca gtctgcctgg tctggggctg attaccagcc agataaggat
      541 gagcagggga ttgaggacca taccatctat gccatcaatg acattggtca atatgagatt
      601 ctcaacgaag acctgtcagg tctggaacag gctgaatcca tcaaggaggt tccgagtgag
      661 cttcaagcca tcgtaagtaa gctgaaagcc ttgacagagc gcctgtccat ccaggccctg
      721 ccgcaaccat ggttgcctcc gctgtctaag gaaatcttcc tgcaagatct acgaccacaa
      781 ggtttcaagg acttgtgggg acaggcaagt ggtttggttg cacgcttggg gatggtggat
      841 ataccgagtc gccagagcca agaagtcttg gaacatgact ttgaaaagga tggacacatc
      901 gccctcttct ctagtccagg tatggggaaa tcgacctttg ttcaaacggt attggttgat
      961 ttattccgac aaaacaatcc agatcgtctt cacacttatc tgctcgattt tgggaccaat
     1021 ggattattgg ctctaaatga ttatccacat gttgcggagt atttcttggt tgaagagact
     1081 gaaaaattac agaagttgat aaaacgactg caagatgaaa tacgagaaag aaggaaatta
     1141 ttcaccgatt cctccgttca taatatcgaa atgtatcgca agttaggaga taggaagtta
     1201 gctgatattg ttattgcggt ggacagctat gatggtctta aaggttcgga agtcgaagtg
     1261 gcgatcgata ccttgcttca aactcttgca agagatggaa tggggcttgg gatctatttg
     1321 atcctaaccg ctagtcgaac aggggcactc aaaaacaatt tgtatagtaa ttttaaacga
     1381 agaatagccc tgaaaatgaa tgatgatagt gaagcgcgag caatcgttgg aagaactgct
     1441 ttggttattg aagatgtgcc gggtagaggg cttattcaag tggatgatcc tgagttgatt
     1501 cagttagcaa tgccggctaa aggtgagaat ggtctcgaga tgatcgaagc attacgtcat
     1561 gaatcccaag aaatggctaa gaattggaat ggagcattac ctcaaggcat tgtcattgtt
     1621 ccagaggttc ttgatgaaca gactttttta caaatggtgg agacagagaa agcaatcgct
     1681 tcagatttta ttccgattgg tttagaattt gaagaggtac acacaatctc gttgccgatt
     1741 cctaagttaa aacatctatt gattttgact gaaaaggaac agcataagtt agtagcagtt
     1801 cgccatcttt taaatatgct ttggcatgtt cgaaaggggc agtctgtgac gctgtttgat
     1861 gtgacagatg aatatggccg atttgctggg aaagtgaagc aatattattc ttcttctgaa
     1921 ctaactgaaa atgtagtgga agagttgaaa atgagagttt tacatgctcg taaacaaaaa
     1981 gaacaattga atgaatttgt catcatcagt gatttaccag cctttatgac agttgatggc
     2041 atgtcagata atgattttgc acttctgtat gaagagggac agcgtgttgg tttgcacttg
     2101 attgttgtag ctaacaagac ttatatgtcg ctgtcgagtg gtattcaacg cttgattaaa
     2161 caaaaattgg atacggtact gatcgcgatg aagatgagca accagtcggt ggtggcacga
     2221 agcgaggttg gacgggaggc agagttggcc atcgatgagg tatacctcca ctatcaagac
     2281 caacaaatca agttgaaaat taccaaagaa atagaatagg agagttagga tgcaaaaaga
     2341 aaaaatgaca gtagaagagt ttttacaact gaagaaggat gctcaggtat ccttgattct
     2401 atttattgtt tttgggcttg tcttgttttc aagtatgttt tatataacag tgattggtgc
     2461 aagaacacaa atgtacaatt cacttgtgac gatggtgttt ctatctgtat tgatgagtgc
     2521 agtcagaccc tatgtttttt ctaaaaatat cttagaaaaa aagcaacctg agctgaagca
     2581 gtattcgaca gagggctact cagtttctag tgcctttcta aaaagggttt ttctagttta
     2641 ttcggttgcg attattgtgg ttgttttcgg ttttgcctct gcctacgcaa ctcgggttga
     2701 gaccatcctt cccgatgata ccttaccaag tctggaacag aagtctatta tcgagcaaaa
     2761 attggagggc aacagtaatg agtgacgagt atttgcttag ttgtattacc aatagtcgtg
     2821 aaaagcttgc aaagtataag cgagtaagga atactattat gagtcataat cttcatgctc
     2881 aacgtagcct atctggtctc caatcttaca ttgagcattg tcagaaggtc attgatagga
     2941 tcgatagtca ggatgggtac ggttatctgg caaactttcg tgataagtta gcggatgaca
     3001 taaaggtttt gaaggactat cggaatttcg tgaaggattc aaatgcatcc tttgttgact
     3061 tataccagac attgaatgca aagataggaa atttgaatgc ttcaatcgca aactataaaa
     3121 gtatgtacaa tgatgggaaa cccgtgtggg aatgggtttg gtaggaggtg ctatggatac
     3181 aaatattaat agtattgaca gccaatatac gtatgataca attgtgtacg acgatggtca
     3241 gatcgatggt gttattcaac agttgaatca gattattgat tgcctgaatc agatttcttc
     3301 agagattgta gggctggaag cacaggatag tagctggtct ggaaaaagca aagctgccta
     3361 tgttgattta aaagagtttc tcaagtccta taggaaagat tatcagcaca gtatcaaaga
     3421 gctcaagtca acggtttcgg gattggaaac ccttttgaac tccatcccta gtgcaacagt
     3481 attgaaggag attgatggcg catgatattt tcaattgaag cattgtacac cctccatctt
     3541 ttgaacgatc attcggatga cttaagtttt cttccgcttc caaatgggca aaacttagac
     3601 cgcaagtcct tgttaaagga aacgctagaa aaaggatttg aggatttgaa agagttacaa
     3661 ttggtggtgg ataatcagtt gactgagcga ggggtggagt atggctacta tctggaagag
     3721 taccattcgt ccccttacca ctgcagagtg gacttatcct atttctgtgc tccggctgtt
     3781 gatgagttta agcgatattc tatacttatc aaagaggttg atgagggggt gtttgtcatt
     3841 gagcgtcttg ccagtgtttg gtttttgggg ttcttgctgg aaactcatcc gattcttcat
     3901 catttggatg ataagatgaa ggattatttg atgtacgatt ggacaccgaa atcccatttt
     3961 agtcttctgg ttgaacatgg tcggtcagag ggacttcgtt tggcggtgga gcaactaggg
     4021 acaatttaca gcgatacact ctttcttgaa attgaagaga gtatctatga gtacgattta
     4081 acacgagaac tcatgcgctc gattgatagt gaaacactgc aatcaaggct tatcaaggat
     4141 ttgaaagttg aggtataaga atgtcagaac agattcaaat ttcgctcagc tctcaggaac
     4201 aaatcatttt acatgctttg cgcattacag agttggcgac agatattacg caaactattc
     4261 agcaagtggt tgaaacaatt cctaattttt ccagccaagg ttccttccat acgatttata
     4321 caactgggaa aaatgatggt ttctatcgct atgtgttgaa agcccaagaa cttaagacac
     4381 tttcagaagt tctctatcgt catgtagaaa cgactcatca gaaaatggtg gacatggata
     4441 gagccttggc tgtgcatatc accaaccagt tcttaaacag cccttcaacg agttctgaag
     4501 ataagcagtt tatccgagaa catccagaag aggcggtgaa gtatatccag agtgagatga
     4561 agaaatcagc accatcatca ggaggtggat catgacacgt ttttatacga atcgctcaga
     4621 ttttgagcag aaccactggc tagattctca aaagagtcga gtcgtcgcta tgttgcggga
     4681 tatcgattcg gtctcttcaa cagtgcagtc ggaaggtctt tctcatctct atcgctctcg
     4741 tggtgaggct agtttaggtg caagcaagcc gagttctact tttagccagg ctgttgaccg
     4801 tgtcgatagc atgttaagaa gcgctggaaa gattcacaag gatattgtcc aaaatattga
     4861 tagtaagttt accaagggct tagacaagag ttttcaaagc ttgaacgaag ttaatggcag
     4921 taagaatcct tatatgtcta aggggaagta tgagaaaact tatacgacta ctaactatac
     4981 tagtaattat ggctatatga cccaaacgca taccagacag gtgcattaca gtctttcaga
     5041 catccttgat ggcacgaaaa gtccaattcc agcagcccag tcattatatc aagaccgagt
     5101 gaagaacctt cgagagcagc tccgccagac cgaccaacta acggataaac aaatcaaaga
     5161 gttggagtcc aagagtgatg cggagttggt tcaggagttc tatccgactc tggagtcaga
     5221 ctatagtcaa atcaaaaata gtacttggca ggaggagaac aaggaagctt taggcttggt
     5281 tgatacaggt ctcaaaattt tggcgggcat tgccctagca gcaggaacgg ttatgactgg
     5341 aggtgctgcc cttccgcttg cacttgcagg tggcgcgtac ctctttgcgg atggagctca
     5401 ctctgcgatt acaggcaaca ccttgattac tggtactcag ttgagcacgg aagagcgtat
     5461 ctgggctgga gctgaggctg ttgtgacatt ggctactttt ggtagctttg tggcaggtca
     5521 aggtgccttg cgagcaggta aacaaatccc gacaaccgtt cagaccattg gtaaattggc
     5581 tggctatgcg gatgagggtg tggaactggg acaattggca tctggtatcc ttagtggaga
     5641 cggtacgaat gcgaccttga attacatcgg aggtaaagca tttgaaggca ttgtagagag
     5701 ctctgttaag ggactccaaa gccaactttc tcacattgaa gtttcaagcc caagccaaat
     5761 tcagctcaac gctcgtcagg gtcagaactt tgtggacttg caaacggttg agtttggtaa
     5821 aatcgccagc aaggtcgaga ataacatcac gatcaagatg acggttgatg gcgttgagca
     5881 gaagatacga gttgatgcga ttggttttga cgaggcagga aatatccgca tccaggaata
     5941 cacaactgcc cagaatggtt tgaaaatcag ccgtcagaac ctcttggaag acctgtccaa
     6001 gtacggtggc accattgtcg gcgcaggcaa gggcgacttt gtcggcggtg ttgagatacc
     6061 caagggcaca cggattgatg tcgtgtcgca gaagacgggg aatgtgacga taggatctct
     6121 ggataccgtt tattctgtaa aagactttaa agctgaaatg gagcaattgc ctcatataaa
     6181 tctgtccaaa aaggttgaag atggcttggt tagtacgaat aacgggagcc gtacgtcgcc
     6241 tcaccatgtt tattcagtag aagaaattgc aactcatctt gctatgttcg atgatggagc
     6301 agtgaaaatt atgagctttg aatcatatga ttatgggttg caaaattatg gtgcgacgat
     6361 aggccatcct gacggtcatt acgtaatgcc taaatttgtt tatgaaaaag ctgtaagggc
     6421 atctaatggt aatccaagag ttttagaggg actattggga ttagaacagg ggtacctagg
     6481 aaataatcca gttgttatag atattcagcg cttaaaaaac ctccggatgc cttctggtaa
     6541 cgagcctgga gcttggcaag gtttgtggga acctggaggc tacactcagg gggggattcc
     6601 tgaggcagtg actgatcagc ttagtgagtc ggattataca atagacgaag tattcaaata
     6661 gaaaggtcag atatatatga aagtgttacg agggactgac tttataatat atgaattaaa
     6721 tgggcaatat agcattaatt ggtatcgtgg gcgagaacga ttctcttatc caatagatga
     6781 agatttagta aaaaaagctc tgttaacatc taaagacggg aaagaggtca tgttttatgt
     6841 agagcatggg cgctggccag aagaggatga gttagagcat tataatgaat ctaacgtaat
     6901 tacacataga gggaataatt ttattgtcta tgaggaaaat ggtaaatatg agatgcactt
     6961 tatgactggc ggatttcaag accgagaggt catctatcct atttctaaag aacttatgga
     7021 aagagctttt gagtcgcctc aagatgccta tgaagtaaaa atttacttaa tgacaggcca
     7081 ttggccagat aagagtcaag aagaattgga tagaaatttc ttaaggcagt ttccagagca
     7141 aattcttaag aacccgacta ttccaaagac tctcttttca gagtatgagt ttacgaaatt
     7201 attgaaacaa gcaattgaaa cagtattaca gactagtaaa attgatagct taggaatagt
     7261 tgaagaacat ttggaactct tgcttctaga cccaatcaag tggcaggaag aaacagaaat
     7321 tattcatttg cagttattgc aagaaaagtt gaacaactac attcacttta tcgaaagcaa
     7381 gcagtatgtg gaaagttacg gggatgactt tacagaaaaa gttatcaatc ttactttcca
     7441 gtatgctccg tcagacaatg gtcttgcttt ccttatgcaa gttcaaaaag ttctacagcc
     7501 gacagatatt cggttgaagg ttgtagtgcc agagtagagt tttaagtcca gacagggtca
     7561 ccttgtctgg attttttggt ataatggaag ggtagaaata tttttcattt gtgcataaac
     7621 ggttaggcta gacttggtct aaagcggagc ctaggagtgg gaatgagatt ggagttgtta
     7681 tctctcgcat ccattttgaa acaagttgag ccgctctgct attaccacaa attcagctca
     7741 acgctcgtca gggtcaggac tttgtggact tgcaaacggt cgagtttggt aaaatcgcta
     7801 gcaaggtcga gaataacatc acgatcaaga tggcggttga tggcgttgag cagaaaatct
     7861 gagtggatgc gattggcttt gacgaggcag gcaacatccg cattcaggaa tacacgactg
     7921 ctcagaatgg tttgaaaatc agtcgtcaga acctcttgga agacctgtcc aagtatggcg
     7981 gcaccattgt cggcgcaggc aagggcgact ttgttggcgg tgttgagata cctaagggca
     8041 cacggattga tgtcgtgtcg caaaaaacgg ggaatttttc aatagatagc actcctaact
     8101 atatccaagt aggacgttat actacagaac tttctaagat agacctgcca cttgaggaaa
     8161 aagttataag acttcaagag ttttattcag atctttcgga taagacagat attaacgttc
     8221 catcggatcc tcaatatgta gtggcggtac gcgacggttg ggttgagtat gattggccaa
     8281 agaatttggg ttatcaagaa ggtaccgttc agtcaattac aagagattca ggattgccag
     8341 atcaatggga tcgttttgga catatgggag gagggaactt ctcggacatc cctagtgatg
     8401 gtccctatac ctatagtcag cgtgccattc cgtatgtaga aaatccgaat gcttatcata
     8461 aaggaacctt tataagatga gttatacaga ctttataaaa ctctaccaag aatcactcaa
     8521 agtaggggtc caactgataa ttggtgcaca aaaatcatcg ttactcaaga cagatttatc
     8581 tattaagtat attaaagaga atctggtgac ggcaatcgtt gctcaaagac tttatgatca
     8641 aagtattgta caacataaaa tgacaagtcg tgaggagact ttgaaagtag atgaagtgta
     8701 tctatatcat gaccaagact atcaaaaagt aaaaattagt aagcaggtgg tggagtaatg
     8761 aatcgaataa aaaaactaat gatgtgtatt ttaggaggtg ttacaataat gggtttgaca
     8821 ggttgtaata aagaagtaga gattagaaca gagttggcac cgatgtatga ggtgttggac
     8881 cagcaatcta tagagaattt tgatattttt tcgattgaag ataatttgag gatatatggt
     8941 atggagagtg ccaaggcatt taagactgac ttggctatta gcccagatgg acaatttgaa
     9001 ggaatgagtt atgatttgtc agtttctgaa acggaaggct atccaacgac ttatatagat
     9061 ggaaatttga aaattaatac gactagtgaa gtgttatccg atagaaaaat gatgtttgaa
     9121 gaatttcatt tttcagaaga ctatttcaat agtttagagt tagtaaaa
//
LOCUS       DBNFEG010000080         9155 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.080, whole genome shotgun sequence.
ACCESSION   DBNFEG010000080 DBNFEG010000000
VERSION     DBNFEG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9155
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.080"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(141..1292)
                     /gene="recA"
                     /locus_tag="AC1PNG_001253"
     CDS             complement(141..1292)
                     /gene="recA"
                     /locus_tag="AC1PNG_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIP6701760.1"
                     /translation="MAKKPGKKLEDITKKFGDERKKALDDALKSIEKDFGKGAVMRLG
                     ERAEQKVQVMSSGSLSIDIALGAGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKDGG
                     IAAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSV
                     AALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTVAIFINQLREKVGVMFG
                     NPETTPGGRALKFYASVRMDVRGNTQIKGTGDKKDQNVGKETKVKIVKNKVAPPFKEA
                     VVEIMYGEGISRTGELIEIGSNLGIIQKAGAWYSYNGEKIGQGSENAKKFLADNPAIF
                     DEIDRKIRVHYGLIEADGVEEVATEEAPVVAEEIQDVILDLDGGIELED"
     gene            complement(1344..2525)
                     /locus_tag="AC1PNG_001254"
     CDS             complement(1344..2525)
                     /locus_tag="AC1PNG_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIP6701761.1"
                     /translation="MKAELIAVGTEILTGQIVNTNAQFLSEKCAELGIDVYFHTAVGD
                     NEQRLLSVLEVARQRSDLVILCGRLGPTEDDLTKQILATFLGRNLVFDELAMAKLDRF
                     FASRPGRVRTPNNERQAQIVEGSQALQNPAGLAVGGMIEQDGVTYIVLPGPPSELKAM
                     FSESLLPLLSQSQQQLYSRILRFFGIGESQLVTVLADLIDKQTDPTLAPYAKVGEVTL
                     RLSTKATSQEEANLRLNQLEEEILQHDKLADYFYAYGEDNSLVKTVATRLAEKRQTIA
                     IVEQGTGGLLQAELSLALADQPYFSGGKVIGQLGTESGRLSEEADCIRQELQADLGLA
                     VSVLIKPESTEDNVLAKVYLTLATTSGISQKEIDLGGYSWQYLRQLACLQAWDFVRNT
                     L"
     gene            complement(2562..3131)
                     /locus_tag="AC1PNG_001255"
     CDS             complement(2562..3131)
                     /locus_tag="AC1PNG_001255"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIP6701762.1"
                     /translation="MSRCAWVNLNNPLYIAYHDEEWGKSLHDEQSLFELLCLESYQAG
                     LSWEIVLNKRQAFRSAFFNYDIQKVAAMTDSELDSLLTNPDIIRHKAKLYATRANAQA
                     FLRVQEEFGTFDTYLWSWVDFTPLDNPIKSFRELPTKSELSERLSKDLKKRGFKFVGP
                     VCIYSYLQAAGLLNEHEETCEIGKKLRTN"
     gene            complement(3385..3975)
                     /gene="ruvA"
                     /locus_tag="AC1PNG_001256"
     CDS             complement(3385..3975)
                     /gene="ruvA"
                     /locus_tag="AC1PNG_001256"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIP6701763.1"
                     /translation="MYDYIKGILTKITAKYIVVETQGVGYILQVANPYAYSGQVQQEV
                     TVYTHQVIREDAHLLYGFATENEKSVFLSLISVSGIGPTTALAIIAVDDNDGLVRAIE
                     QKNITYLTKFPKIGKKTAQQMILDLEGKFVMSEEAGPVQQVAPSSENIALDEAMEAME
                     ALGYRPAELKKIKKFFEGTNDTAENYIKSALKMLMK"
     gene            complement(4014..5951)
                     /gene="mutL"
                     /locus_tag="AC1PNG_001257"
     CDS             complement(4014..5951)
                     /gene="mutL"
                     /locus_tag="AC1PNG_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIP6701764.1"
                     /translation="MSHIIELPEILANQIAAGEVIERPASVVKELVENSIDAGASQIE
                     ISVEEAGLKMIQITDNGEGIAPDEVALALRRHATSKIKNQSDLFRIRTLGFRGEALPS
                     IASVSHMIIETATADSAHGLHLEAKGGVIEKEEPVSRPVGTQITVSDLFYNTPARLKY
                     VRSQQAELSHIVDVVNRLSLAHPEMAFALVNEGRELIRTAGTGKLRQAISGIYGIASA
                     KKMVEIEAEDLDFQISGYVSLPELTRANRNYISIFINGRYIKNFLLNRAILEGYGSKL
                     MVGRFPLAVISIEIDPYLADVNVHPTKQEVRISKEKELMTLIREAISQALKEQDLIPD
                     ALENLAQSSTRPKVKAEQGTLPLREPKIYYDTIKQDFFLKPDVVAEDVKPLEEDRQEI
                     VESPAENKPTSVQFAERQSVESEDQEHPNLSAKELAKLADKLDREATSTFPELEYFGQ
                     MHGTYLFAQGKTGLYIIDQHAAQERVKYEYYREKIGQVDNSAQQLLVPYIFEFPQNDA
                     LDLVHKMDALRQVGVNLEEYGSNQFILREHPIWMKEEEIESGIYEMCDMLLLTDQVSI
                     KQYRAELAIMMSCKRSIKANHALDDYSARDLLRQLSYCQNPYNCPHGRPVLVHFSKSD
                     MEKMFRRIQENHTSLRELGKY"
     gene            complement(6069..7592)
                     /locus_tag="AC1PNG_001258"
     CDS             complement(6069..7592)
                     /locus_tag="AC1PNG_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol oxidase"
                     /protein_id="HIP6701765.1"
                     /translation="MTELDVVSDVVTHPEETYRRSRRASRRQQVQLNGEFHERVLKTK
                     YWPALVWSLVLSVFSVANPLLMPFATNMQTQNLYAGMAMANGQIPYGDFFGTSGLLFY
                     LLAFLGHLGGTFIIFGILQFIALLIAGVYFYKVVAYFSQSEHLATSSSHWFYVFIFAL
                     GFGGMYAEMFALPFLLTSVWFLVRYFENAVRDEAFILYGIDAALVFLIYPKSLILWLV
                     AGLVLFIFNIQHRQVTRGIYQLLATIFGFLLILYAVGYYAFEAQILGTAIQQTFLYNL
                     QLDFHHSYLYLALAIVSVFLLLSGFFKSFIQMVFSFKQGRHTYIKVLLLLTFLVQCVF
                     IIGNANFQWSQLILLLPYGFAMSVVYLRDEDVEDYRGYLRRQFFLPLAICLGIIAQPA
                     YLYLVQGDLRTDREQVANYIGEQTKDSDKIYVWDNSASIYLSSQRLSAATITTAEPYL
                     NTDDNKNSLMYNINKNEAKFVVVNKNLPILDEIKTNLESQYQSVQTTDYFTIYQKNE"
     gene            complement(7761..9002)
                     /locus_tag="AC1PNG_001259"
     CDS             complement(7761..9002)
                     /locus_tag="AC1PNG_001259"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIP6701766.1"
                     /translation="MLENWLNTKQGQVFHYKMEKIEYALELLGNPQFAVPVIHVAGTN
                     GKGSTIAFMRQLFQTHGLRVGSFVSPHMVSVHDRICIDSQPISDHDFQHYLQKVYDLE
                     QEVATRYEPFRYFEVMVLIMFLYFEAQQPDVALVEVGIGGLLDTTNVVAPALSVITSI
                     GMDHQDLLGSTLGEIAEQKAGIIKENVPVVLGPLCPETTAICRHIAQDKQASVYQFGQ
                     EFTYKAGQFSNADLELSELVLGLAGHHQEENAAVALQTFLLYMTNIQKDIQPQLIQQA
                     LAQTSWPGRLELVAQEPKIYLDGAHNVPAIERLIEFIQVQEEPVTILFSALRRKDFQE
                     MLELLDEKLPHTALVLTSFAYDGALSEENRQGRDYVENYRQFIEAWQSSKQGILIVTG
                     SLYFISEVRRIFKNEYLFANK"
BASE COUNT         2626 a         2111 c         1743 g         2675 t
ORIGIN      
        1 aattttgggg taaaaattcg gtttttatta gaaaaaagtc tggaatctcc agactttttc
       61 gttcttatac taaacaactt cgtacacacc tataacagat atgcacaaat agttcaatat
      121 taaatctatt ttgttaggaa ttaatcttct aattcaatac cgccatcaag atctaggata
      181 acatcttgga tttcttccgc aacaacagga gcttcttcgg tcgcaacttc ttcaactcca
      241 tctgcttcaa tcaagccata atgaacacgt atcttgcggt caatttcatc aaagattgct
      301 ggattatctg ctaagaattt cttagcattt tcagaacctt ggccaatttt ttctccatta
      361 tatgaatacc aagcacctgc tttttggatg atgccgaggt tactaccaat ctcaatcaat
      421 tcacctgtgc gagaaattcc ttcaccgtac atgatttcaa caacagcttc tttaaatggt
      481 ggcgccacct tgttcttcac aatcttcacc ttggtttcct taccaacgtt ttgatctttc
      541 ttgtcgcctg tccctttgat ttgtgtgttt ccacgaacat ccatacggac agatgcatag
      601 aatttaagcg cacgtccacc aggtgttgtt tcagggttac caaacatgac accgactttt
      661 tcacgcaact ggttaatgaa aatggctacc gtcttagttt tgttgatgga tgctccgagt
      721 ttgcgcatag cctgactcat catacgagcc tgcagaccaa catgactatc accaatatcg
      781 ccatcgattt ctgcacgtgg tacaagggct gcaaccgagt caacaacaac caagtcaacc
      841 gcaccagagt cgatcagctt gcctgcaatt tcaagacctt gttcccctga gtctggctgt
      901 gacaatagta actcatcaat attgacacca agagctgctg cataggctgg atccaaggcg
      961 tgttccgcat cgataaaggc agcaatacca ccatctttct gggcttgagc tactgcatga
     1021 agggcaacag ttgtcttacc tgaactctct ggaccataaa tctcaatgat acgacctttc
     1081 ggatagccac ccgctccaag cgcaatgtcg attgacaaac tacctgagct catgacttga
     1141 actttttgtt cagcacgctc accaagacgc ataacagcac ccttaccaaa atctttttca
     1201 attgatttta gggcatcatc cagcgctttt ttacgctcat cgccaaattt ctttgtaata
     1261 tcttctaatt tttttcctgg tttcttagcc aatgattttc tcctcttttc ttagtcaaac
     1321 tattatagca aatatctgct atttcacaaa gtgtttcgta caaaatccca ggcctgcaga
     1381 caagcaagct ggcgaaggta ttgccacgaa taaccaccta aatctatctc tttttgggaa
     1441 atacccgagg tcgtagccaa agtgagatat acttttgcaa gtacgttgtc ctctgttgat
     1501 tccggtttga taagcacaga cacagccaaa cccaaatcag cttgcagctc ctgccgaatg
     1561 cagtcagctt cctctgatag cctgcccgat tctgtcccta gctgaccgat gacttttcct
     1621 ccgctaaaat acggctgatc agccaaagcc aggcttaatt cagcttgcaa gagaccacct
     1681 gtcccctgtt cgacgatagc gatggtttgt cttttctccg ccaaacgagt cgctaccgtt
     1741 ttgaccaaac tattgtcctc tccgtaggca tagaaatagt ctgccagttt gtcatgttgt
     1801 aagatttctt cttccaactg attcaaacgt agatttgcct cttcttggct ggtcgctttg
     1861 gtggacaagc gtaaagtcac ctctccaact tttgcataag gagcaagagt tgggtctgtc
     1921 tgcttgtcga tcaagtccgc caaaacagtc accaactggc tttcaccaat gccaaaaaaa
     1981 cgtaggatac gcgaataaag ttgctgctga gattgggaca gtaaaggtaa gagactctca
     2041 gaaaacatgg ccttgagctc acttggtggg ccaggcaaaa caatataggt tacaccatct
     2101 tgctcaatca taccaccaac agctaaacca gctggattct gtagcgcctg acttccctcc
     2161 acaatctgtg cctgacgttc attatttggt gtacggacac gacctgggcg actggcaaag
     2221 aaacggtcta attttgccat agccaactca tcaaacacaa gatttctacc taagaaggtc
     2281 gccaggatct gcttggtcaa gtcatcctca gttggaccca atcttccaca gagaatgacc
     2341 aggtcgctcc gctgacgggc tacttccagt acagacagaa gcctctgttc attatctcca
     2401 acagctgtgt ggaaatagac atcaattccc agctctgcac atttctccga aaggaactga
     2461 gcattggtat taacgatttg acctgtcaaa atctccgtcc caacggcgat tagttctgct
     2521 ttcatattcc tcctctgtct agggtgcgat tgctcgctca tctaattagt tcgtaatttc
     2581 tttccaatct cgcaagtttc ttcatgttca ttcaacagac ctgctgcctg caaataagaa
     2641 taaatacaga caggacctac aaacttgaaa cctcgtttct tcaaatcttt ggacaatctt
     2701 tctgatagtt cacttttggt cggtagctct cgaaaggact taatagggtt gtcaagaggt
     2761 gtaaagtcaa cccaagacca aagatacgta tcaaacgtgc cgaattcctc ctgtactcga
     2821 agaaaggcct gagcattggc gcgggtagcg tatagctttg ccttatgacg aatgatgtct
     2881 ggattggtca gaaggctatc taactcgcta tctgtcatag ccgcaacctt ttgaatatca
     2941 tagttgaaaa aagcagagcg aaaagcctgg cgtttattga gaacaatttc ccaggaaagt
     3001 cctgcttggt aggattccaa gcaaagcaac tcaaacaaag actgctcatc gtgaagtgac
     3061 ttgccccatt cttcatcatg gtaagcaata taaagtggat tatttagatt aacccaagca
     3121 caacgggaca tagaaactcc ttttcgtgaa taactaaagt gcgaatcagg ataaactgac
     3181 tgaaacacgg ctacgatact ttgtaaaatt gtcaaactga atcctaaata tcaccgaata
     3241 acgcagttga catctggcaa aaggcttcgt caaaactcgc cttttaccta gttgtcattg
     3301 cggaggtctc acaacgaaat ttctagcaaa atttctgttc ggccgtctat tttgctttgt
     3361 atcgcttaac gccttagtat cctattactt catcagcatt ttaagggctg acttgatgta
     3421 gttttcagcg gtgtcattgg tgccttcaaa gaatttcttg attttcttga gttcggctgg
     3481 gcggtaacca agggcttcca tggcttccat agcttcatca agagcgatat tttcactgga
     3541 tggtgctact tgttgaacag gacccgcttc ttcgctcatg acaaacttgc cttccaggtc
     3601 caaaatcatc tgctgggctg ttttcttgcc aatcttcgga aacttggtca ggtaggtaat
     3661 gtttttctgc tcaatagcac gaacaagtcc atcattatca tcaacagcaa taatagccag
     3721 agccgttgtt ggaccaatac ctgatactga aatcagactc agaaagacgg atttttcatt
     3781 ttctgtagca aatccgtaga gcaaatgagc atcttctcga atcacttgat gagtatagac
     3841 cgtcacttct tgctggactt gtccagagta tgcgtaggga ttagcaacct gcaagatata
     3901 gcctactcct tgtgtttcta ccacaatata ttttgcagtt atttttgtta aaattccttt
     3961 aatatagtcg tacatatact tcctttacta atgcgtcaac tttttcaact atgttaatac
     4021 ttgcccaatt ctcgcaaact cgtgtgattt tcctgaatgc gacggaacat tttttccata
     4081 tccgatttgc taaaatgtac taagactggg cgaccgtgcg ggcagttata gggattttgg
     4141 cagtaagaca attgtctcaa taaatcacgc gccgaataat catccaaagc atggttggcc
     4201 ttgattgacc gtttacaaga catcatgata gccagttctg cccgatactg cttgattgac
     4261 acctgatccg tcaagagcaa catgtcgcac atctcataaa tgccagactc aatctcctct
     4321 tccttcatcc aaataggatg ttcacgcaga ataaattgat tagatccata ttcttccaag
     4381 ttgacaccaa cttgacgaag ggcatccatt ttgtggacta ggtcaagggc atcattctgc
     4441 ggaaattcaa aaatataggg caccaataac tgttgagccg aattgtcaac ctgtccaatt
     4501 ttctcacggt agtattcgta tttgaccctc tcttgtgcgg catgctggtc aatgatataa
     4561 agtcctgtct taccttgcgc aaataaatac gtcccatgca tttgaccaaa atattctaac
     4621 tctggaaatg tcgaggtcgc ttcccggtct aatttatctg ccagttttgc caattctttt
     4681 gcacttagat taggatgctc ctgatcttct gattccaccg actgacgttc tgcaaactga
     4741 acagatgtag gtttattttc ggcaggcgac tcaacaatct cttgcctatc ctcttcgaga
     4801 ggtttgacat cctcagcgac cacgtctggt tttaagaaga agtcttgctt aatcgtatca
     4861 taatagattt ttggctctct gagtggtaag gtgccttgct ctgcttttac ttttggtcga
     4921 gtacttgact gagctaaatt ctcaagagca tctggtatca agtcctgctc tttaagcgct
     4981 tgactaatcg cctctcgaat caaggtcatg agttcttttt ccttggagat gcgaacttcc
     5041 tgcttggtcg gatgcacatt gacatcagca agataggggt caatttctat agaaatcact
     5101 gccaacggaa agcgtccaac catcaactta ctgccgtaac cttccaaaat cgctcgattc
     5161 agcaaaaaat tcttgatata gcgaccattg atgaaaatgg aaatgtagtt gcggttggca
     5221 cgagtcaact ctggcaagga aacataacct gaaatctgaa aatccagatc ttctgcctca
     5281 atttcaacca tttttttggc agaagcaatc ccataaatcc ctgaaattgc ctgacgaagt
     5341 ttccctgtcc ctgcagttct aattaattct cgtccttcgt ttactaaggc aaaagccatt
     5401 tccggatgag ctagactcaa tcgatttaca acatcaacaa tatgtgataa ttcagcctgt
     5461 tgactgcgaa catatttgag acgagcaggg gtattgtaaa ataaatccga aacagtaatc
     5521 tgcgtaccaa ctggacgact aactggctcc tctttctcga tgacaccacc tttggcctcc
     5581 aaatgaagtc cgtgtgcaga gtctgccgta gctgtctcaa tgatcatatg actgacagaa
     5641 gcaatggaag gcagagcttc tccacgaaaa ccgagggtgc gaatacgaaa caaatccgat
     5701 tgatttttta tcttactggt ggcgtgacgg cggagggcaa gcgctacttc atcaggggca
     5761 atcccctcac cgttatccgt aatttgaatc attttaaggc cagcctcttc aacactaatt
     5821 tcaatctgac tagcccctgc gtcaatggaa ttttccacca actccttaac cacactggct
     5881 ggtcgctcaa tgacctcacc agccgcaatc tggttggcta gtatttctgg tagttcaata
     5941 atatgtgaca tcatttctcc tacatttctt taataatttt tcccgaatgc ttatttttac
     6001 attataccac atatccactt tccctccttt tcccagatag gctaaagaaa aaatgactgc
     6061 ctgagcaatc attcattctt ttgataaatc gtaaagtaat ccgttgtctg aacagactgg
     6121 tactgtgatt ctaaatttgt cttgatttca tctagaattg gcaaattctt attgacgact
     6181 acaaattttg cctcattttt attgatgtta tacatcaagc tatttttatt gtcgtcagta
     6241 ttcagatagg gttctgctgt agtaatcgtt gcggcagata aacgttgact tgaaagatag
     6301 atgcttgcgc tattatccca aacataaatc ttatccgaat ctttcgtttg ttcaccaatg
     6361 taattggcaa cttgttcacg gtcagttctc aaatctccct gtaccaaata aagataagca
     6421 ggttgagcga taatacccaa gcaaatagct aacggaagaa agaattgacg acgcaaatac
     6481 ccacgataat cttctacatc ctcatcacgc aggtaaacga ctgacatcgc aaaaccatac
     6541 ggtaaaagaa gaatgagttg gctccattga aaattagcat tcccaataat aaagacacat
     6601 tgaactaaga aagtcaataa taggagaact ttgatatagg tatgtcttcc ttgtttgaaa
     6661 gaaaacacca tttggatgaa acttttaaag aatccagata agagtaaaaa tacagacaca
     6721 atcgccaagg ctaaatatag ataggaatgg tgaaagtcta actgtaagtt ataaaggaaa
     6781 gtttgttgaa tagctgtccc cagaatctgt gcctcaaaag cataataacc taccgcatat
     6841 agaatcagaa gaaaaccaaa aatggtcgct aataattgat agattcctcg agtaacttgg
     6901 cggtgttgga tattaaaaat aaataaaact agacctgcaa ctaaccaaag gattagactt
     6961 tttgggtaaa ttaaaaatac caatgccgca tcgattccgt agaggataaa tgcctcatca
     7021 cgcactgcat tttcaaaata gcggaccaga aaccacacac tagtcaaaag aaatggaaga
     7081 gcaaacattt ctgcatacat ccctccaaaa ccaagtgcaa aaataaatac atagaaccaa
     7141 tgactagaac ttgtcgctag gtgttctgac tgactaaaat aagctacaac cttatagaaa
     7201 tagactcccg ctatcagtaa tgctataaac tggaggattc caaagataat gaaagttcct
     7261 cccagatgac ctaagaaagc aagtaaatag aataataacc cgcttgtacc aaaaaaatca
     7321 ccataaggga tttgtccatt tgccattgcc atccctgcat ataaattttg agtttgcata
     7381 ttggttgcaa atggcattag cagtggattg gctacgctaa aaacacttaa aactaagctc
     7441 caaacaagag ctggccaata ctttgtcttt aatactcttt catgaaattc accattcagt
     7501 tgcacttgct gtctgcgtga tgctctacga ctacgccgat aagtttcctc aggatgagta
     7561 actacgtccg agactacatc taattctgtc attttcttct ccttggattt gctttcctta
     7621 ttatatcaaa aagcactccc gatgtcattt cctatcggga gttctggaaa tttttgcaaa
     7681 ctgttctaat tggcgaaagg tatggcgttt gttctcttga aattctttta aaaaggctac
     7741 tttttctgca tgtgtcatac ctatttattc gcaaaaagat attcattttt aaaaatccga
     7801 cgaacttctg agataaagta gagagagcct gtgacaatca aaatcccttg cttgctagat
     7861 tgccaagctt ctataaactg ccggtaattt tcaacataat ctcgtccttg tcggttttcc
     7921 tccgacaagg caccatcata agcaaagctg gttaatacaa gggcagtatg tggtaatttt
     7981 tcgtccaata attccagcat ttcttgaaag tccttgcgcc gaagggccga aaagagaatg
     8041 gtgacaggct cttcttgaac ctgaataaat tcaatcaagc gttcgatagc ggggacattg
     8101 tgggctccat ccaaatagat ttttggctct tgagcaacca attccaaacg accaggccag
     8161 ctagtttggg caagggcttg ttgaatcaac tgaggttgaa tgtctttttg aatattggtc
     8221 atatagagta gaaaagtttg tagggctacg gccgcattct cttcttgatg atggcctgcc
     8281 agacccagaa ctaattctga cagttccaag tccgcattgc tgaactgtcc tgctttatag
     8341 gtgaattcct gaccaaattg gtagacagag gcttgtttgt cttgggcaat gtggcgacag
     8401 atggctgtgg tttctggaca aagtggtcct aagacgacag gcacgttttc cttgataatc
     8461 cctgctttct gctctgctat ttcccctaaa gtcgagccta gtaaatcctg atggtccatg
     8521 ccgatggagg tgatgacgct gagagccggt gccacgacgt tggttgtatc caaaagccct
     8581 ccgataccca cctctactag tgccacatcg ggttgttgag cttcgaaata gaggaacata
     8641 atgagcacca tgacctcaaa ataacggaag ggctcatagc gtgtagcgac ttcctgctcc
     8701 aagtcgtaga ctttctgtaa atagtgctga aaatcatggt ctgaaatggg ctggctgtca
     8761 atacaaatcc tgtcgtgtac actcaccatg tgaggagata caaaactacc gacacgcaag
     8821 ccgtgagtct gaaagagctg acgcataaag gcaatggtcg atcccttgcc attggttcca
     8881 gcaacatgaa tgaccggaac tgcaaactgg ggattcccta gcagttctag ggcatactca
     8941 atcttttcca tcttgtaatg aaaaacctga ccttgtttgg tgtttaacca attttctaac
     9001 atagccacct cctacatact gtctagtata acaaaaaagc ctaacggatg ttggcttttt
     9061 gaaaatatcg tactccacga cttaaatccc cataaaataa acgctctatg ctgagtattc
     9121 aattatttat tttacccacc agttctcatc accaa
//
LOCUS       DBNFEG010000081         9119 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.081, whole genome shotgun sequence.
ACCESSION   DBNFEG010000081 DBNFEG010000000
VERSION     DBNFEG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9119
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.081"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(205..1122)
                     /gene="whiA"
                     /locus_tag="AC1PNG_001260"
     CDS             complement(205..1122)
                     /gene="whiA"
                     /locus_tag="AC1PNG_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HIP6701767.1"
                     /translation="MSFTVQVKEELLLQSSQNKSELSAMIKLSGSLGLASSGLTLSIS
                     TENAKIARHIYELLLHFYQIKAEIRHHQKPNLKKNRVYAVLIEDGVNEILNDLHLADS
                     FFGLETGISPLVLENDSWSQAYLRGAFLAAGSVKDPEKGKYQLEIASVYSDHANDLAN
                     LMQKFLLDAKVIERSKGTITYLQRAEDIMDFLLVIGAEETKTEFENVKLLREARNDLN
                     RATNAEAANIAKTVNASMKTINNIIKIMDTIGLDQLSGDLQEIAQLRIQHPDYSIQQL
                     ADSLTVPITKSGVNHRLRKINKIADELTE"
     gene            complement(1119..2093)
                     /locus_tag="AC1PNG_001261"
     CDS             complement(1119..2093)
                     /locus_tag="AC1PNG_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HIP6701768.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLRDKDVEITAIVTVADDGGSSGE
                     IRQALQVTPPGDLRNVLLAMSDMPKLYEQIFQYRFADSDGPLAGHPLGNLIIAGISEM
                     QGSTYNAMRLLTRFFHTTGRIYPSSEQALTLHAIFTDGTEVAGESKISKHNGMIDHVY
                     VTNSYNDDEPKASRQVVETIMESDMIVLGPGSLFTSILPNLMISDIGKALKETKAEVT
                     YVCNIMTQRGETEFFSDADHVAVLNAHLAEQFIDTVLVNIEPVPQEYMNSNQFDEYLV
                     QVKHDFAGLQKQANRVISSNFLRLENGGAFHDGDLVVEELLKILQVRP"
     gene            complement(2090..2977)
                     /gene="rapZ"
                     /locus_tag="AC1PNG_001262"
     CDS             complement(2090..2977)
                     /gene="rapZ"
                     /locus_tag="AC1PNG_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIP6701769.1"
                     /translation="MSDKLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPTLLPKF
                     LELIRHSQDNNKIALVVDMRSRSFFSEIREVLDEIEGAEDLDFKVLFLDATDNELVAR
                     YKETRRSHPLAADGRVLDGIQLERELLAPLKNMSQNIIDTTELTPRNLRKAISEQFAS
                     QDNQPSFRIEVMSFGFKYGLPLDADLVFDVRFLPNPYYKLELRNLTGLDAPVFDYVME
                     HQESEEFYSHLLGLIEPILPGYQKEGKSILTIAVGCTGGQHRSVAFAKRLADDLEKNW
                     NVNRSHRDKDRRKETVNRS"
     gene            complement(2999..3376)
                     /locus_tag="AC1PNG_001263"
     CDS             complement(2999..3376)
                     /locus_tag="AC1PNG_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIP6701770.1"
                     /translation="MKTIHTDKAPAAIGPYVQGKVVGNFLFASGQVPLSPETGEVVGE
                     TIQEQTEQVLKNIAAILSEAGTDFDHVVKTTCFLKDMNDFVAFNKVYKTAFSADFPAR
                     SAVEVARLPRDVKVEIEVIAVID"
     gene            complement(3560..4879)
                     /locus_tag="AC1PNG_001264"
     CDS             complement(3560..4879)
                     /locus_tag="AC1PNG_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIP6701771.1"
                     /translation="MKQESLGKEIIRLSLPATVENIFQTLVGFVDTLLIAQLGLVAVT
                     AVGLANTILNVYLAVYMALGVGATALIARSIGAGDRESLTFHVRQALVLSVGVGVLFG
                     LLSLVFGRQMLVLMGADAESLAGAQAFFYWVGGLTIFQALMTILGTILRASGDTVSPM
                     KMSLLTNGFNVVLDYFLIFGIGSWSGLGIVGTALGTVLARLLGTVLLYRKVQQTDLAV
                     DLKQLFHLGSPKEMVDLTLPAAAERLVMRLGQVVYFSLIVGLGTTVYASHMIAGNIES
                     FTYMPAYGLATAAAVLIGQALVKGDILTVRRVAFLSSAYGVAIMFLLGTILFFGAPSF
                     ALLFTKDLEAVHQVVTALRIDAFNQPGLAVSLNMAGALQGLGDTKSPLYSTVIGMWGL
                     RVVGAIVLGQMFGLGIAGVWLSILIDLLLRAIFLTWRFHVKTRKLAE"
     gene            complement(5285..5554)
                     /locus_tag="AC1PNG_001265"
     CDS             complement(5285..5554)
                     /locus_tag="AC1PNG_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE family
                     toxin"
                     /protein_id="HIP6701772.1"
                     /translation="MAYKLVLSDDALKQLKKMDRHVGMMLAKDLKKRLDGLENPRQFG
                     KALVGDYKGLWRYRVGNYRVICDIIDNKMVILALEIGHRKEIYKK"
     gene            complement(5544..5771)
                     /gene="relB"
                     /locus_tag="AC1PNG_001266"
     CDS             complement(5544..5771)
                     /gene="relB"
                     /locus_tag="AC1PNG_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HIP6701773.1"
                     /translation="MGIVSLRLNDAEEEIFRSYAIHTGKSLSELFKTALAEQIEDQLD
                     YEIGIKALKKFQENPVTHSIDDVLAELEDGL"
     gene            complement(6043..7389)
                     /gene="asnS"
                     /locus_tag="AC1PNG_001267"
     CDS             complement(6043..7389)
                     /gene="asnS"
                     /locus_tag="AC1PNG_001267"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIP6701774.1"
                     /translation="MSRKLITINQVKDYVGQEVTIGAWVANKSGKGKLAFLQLRDGTA
                     FFQAVALKPNFIEKFGEEAGTEKFDVAKRLSQETSVYVTGIVKEDERSKFGYELDVTD
                     LEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAIQQIRNAIIYATYEFFEKNGF
                     IKFDSPILSGNAAEDSTELFETDYFGQPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVIDRAPQALETLERDVD
                     ALKRYIAEPFKRVAYDDAITLLQEHEADEDTDYEHIEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYEFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRLHP"
     gene            complement(7404..8585)
                     /locus_tag="AC1PNG_001268"
     CDS             complement(7404..8585)
                     /locus_tag="AC1PNG_001268"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002267262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIP6701775.1"
                     /translation="MSKLSRRVLEMEESVTLAAGARAKALKAEGRDILELTLGEPDFV
                     TPKNIQEAAIKSIEDGKASFYTVASGHPELKDAVNTYFENFYGYSIERNQVVLATGAK
                     FVLYAFFAAVINPGDEVIIPTPYWVSYADQIKMNDGVPVFVTAREEHNFKVTVDQLEA
                     ARTDKTKVFLLNTPSNPTGMIYSREELEAIGNWAVEHDILILADDIYGRLVYNGNGFT
                     PISSISESIRQQTIVVNGVAKAYAMTGWRVGFAVGNPEIIAAMSKIVGQTTSNLTTAA
                     QYAAIEAFTGPQDTVETMRQAFEERLNTIYPLLAEVPGFEVIKPEGAFYLFPNVKKAM
                     EMKGYTDVTEFTTAILEEVGVAMVTGAGFGAPENIRLSYATDMDTLKEAVARLHTFMK
                     K"
     gene            complement(8582..9079)
                     /locus_tag="AC1PNG_001269"
     CDS             complement(8582..9079)
                     /locus_tag="AC1PNG_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HIP6701776.1"
                     /translation="MEKKIFQLLEWMASKTGQLVLGSFILLSVVTFSIFTIWDIAAAP
                     FNNVRSHAVAVATEYADLQTVNDFSIYNGTETYFCVFGVTSQGEEVAVLIPEASSTVY
                     VYPLVQGISQEEAQAIAKKNGASQVERTILGLRDGKPIWEVKSGTAYYLVEFETGNFI
                     KREGL"
BASE COUNT         2683 a         2237 c         1724 g         2475 t
ORIGIN      
        1 ttcataaaac agaatcattc cagtgaagaa gattgctatc acagcataca aaaaactggt
       61 cctctcgaac cagtcttagt atcgtcgaat gaattaactt tcagacaagg aactgaggtg
      121 caagcaatac tagagtacgg caagccgaga gtgacgatgt atcaaagtta gttcaattgt
      181 ccataacatt ctatgtatac ctatctattc agtcaattca tccgcaatct tattgatttt
      241 tcgcaaacga tggttgacac cacttttagt aatcggtacc gttagactat ccgccaattg
      301 ctgaatggaa tagtctggat gctgaattcg caactgggca atctcctgca agtcacctga
      361 caactgatct agaccgatag tatccataat tttgataata ttattgattg ttttcatact
      421 agcattgacc gtctttgcaa tattagcggc ttccgcattt gtagctcgat ttaggtcatt
      481 gcgagcctca cgaagcaatt taacattctc aaattctgtc tttgtctctt ctgctccaat
      541 caccaaaaga aaatccataa tgtcctctgc acgttgcaga taggtaatgg ttcccttact
      601 tcgctcaatg actttggcat ccaaaagaaa tttctgcatc agattggcca ggtcattggc
      661 atggtcacta tagacagaag ctatttcaag ctggtatttt cctttttcag gatccttaac
      721 agagcctgcc gctaaaaatg ctccacgaag ataggcttga ctccacgaat cattttccaa
      781 aaccaaggga gaaatccccg tctctaatcc aaagaagcta tcagccaaat gcaaatcgtt
      841 caatatctca ttcacgccgt cttctatcaa aactgcatac acacgattct ttttgagatt
      901 aggtttttga tgatggcgaa tctcagcctt aatctggtag aaatggagta acagttcata
      961 aatgtgacgg gcaatctttg cattttccgt gctgatagaa agagtcaaac ctgatgaagc
     1021 taagcccaaa ctacctgata atttgatcat agcagatagc tcgctcttat tttggctaga
     1081 ctgaagcagc aattcttcct ttacttgtac tgtaaaactc atggccgcac ctgcaaaatc
     1141 ttgagcaact cttctaccac caagtcacca tcatggaaag ctccgccatt ttccaaacga
     1201 agaaaattcg aagaaatcac acgattagcc tgtttttgta aacctgcaaa atcgtgtttt
     1261 acctgtacca agtattcatc aaactggttg ctattcatat actcttgtgg aactggctca
     1321 atattgacca aaactgtatc aatgaattgt tcagctaggt gagcattgag aaccgccacg
     1381 tggtccgcat ctgagaaaaa ctcagtctct ccccgttggg tcataatgtt gcacacatag
     1441 gtcacttcag ccttggtttc ttttagggct ttgccaatgt cagaaatcat cagattcggc
     1501 aagattgagg taaaaagaga acccggtcca agtacaatca tgtcactttc cataatcgtt
     1561 tcaactacct ggcgactggc tttcggttca tcatcgttat aagaatttgt cacatagaca
     1621 tgatcaatca tcccattatg cttggaaatc ttgctctcac ctgctacctc ggtcccatct
     1681 gtaaaaatag catgaagggt caaggcctgt tcgctagatg gataaatccg ccctgtcgta
     1741 tggaaaaagc gtgtcaacaa cctcatcgca ttgtaggttg aaccctgcat ttctgaaata
     1801 ccagcaatga tgagattccc tagtggatga ccagccaagg gtccatctga atccgcaaaa
     1861 cgatactgga aaatttgttc ataaagtttg ggcatatccg acatagccaa aaggacgtta
     1921 cgcaaatcac ctggaggggt cacctgcaaa gcctgacgaa tttcccccga agatcctccg
     1981 tcatcagcta ccgtcacgat agcggtaatc tctacatcct tatcccgcaa actcttcaaa
     2041 atgactggaa taccagttcc gcccccgata actgtaattt ttggttttct catgagcggt
     2101 tgaccgtctc cttccgtctg tccttatcac gatgactacg attaacattc caatttttct
     2161 ctaaatcgtc tgccaaacgt ttagcaaagg ctacgcttct atgctgacca cccgtacatc
     2221 caacagcaat cgttagaatt gattttcctt ccttctgata accaggaaga atcggttcta
     2281 tcaagccgag caaatgacta taaaactctt ccgattcttg atgctccatg acatagtcaa
     2341 agacaggagc atctagaccg gtcagattac gcaactccaa cttgtagtag ggatttggta
     2401 agaaccgaac gtcaaaaacc aagtctgcat ccagaggtag tccatatttg aaaccaaagg
     2461 acatcacttc tatgcgaaac gacggctgat tatcttgact agcaaactgc tccgagattg
     2521 ccttacggag attacgagga gtcagctctg tcgtgtcaat aatattttga ctcatatttt
     2581 tcaagggagc caaaagttca cgctctagct gaatcccgtc caaaacacga ccatcagccg
     2641 ccaaaggatg ggaacgacga gtttctttat agcgagcaac caattcatta tccgtcgcat
     2701 ccaagaatag gactttaaaa tctaaatcct ctgccccctc aatctcatcc aacacctctc
     2761 gaatctccga gaagaaggag cgactccgca tatctacaac aagagcaatc ttattgttat
     2821 cctggctgtg gcgaatcaat tctaaaaatt ttggcaagag agtcggtggc atattatcaa
     2881 tggtaaaata gcccaagtcc tcaaaagact gaatggctac cgtcttacca gctcccgaca
     2941 tccctgtcac aattactaaa tggagtttgt ccgacatgac attccctcct tttctcttct
     3001 aatcaataac tgcaataact tcaatctcaa ccttaacatc acgtggcaaa cgagcaactt
     3061 ccaccgctga acgagctgga aaatctgctg agaaagctgt tttataaact ttattaaagg
     3121 ctacaaaatc attcatatct tttaggaaac aagtcgtctt caccacatgg tcgaagtctg
     3181 ttcctgcttc tgataaaatt gctgcgatat ttttcaagac ttgctcagtc tgctcctgaa
     3241 tggtttcacc aaccacttca ccagtttcag gtgacaaagg aacttgacca gaggcaaata
     3301 ggaaatttcc aacaaccttc ccttgaacgt atgggccaat tgctgcaggt gccttatctg
     3361 tgtgaatggt tttcatatct ctatctcctt ttaatttttt atatctatta tagcacaagt
     3421 tctgtataaa acagctcacc tactactatc ctacacctat tttcttttat aaaaaaggaa
     3481 ttgagatagt atcagcaaca tgaaataagg caatccataa tctacttcta aaactacctg
     3541 cttataccca aaaactagcc tattcagcca gttttcttgt ttttacatga aacctccaag
     3601 tcagaaaaat cgctcgcaag agcaggtcaa tcaaaatcga cagccagacg ccggcaattc
     3661 ctaaaccgaa catctgacct aagacgatag cacctacgac gcgcagcccc cacatcccta
     3721 tcactgtgga gtagagtggc gacttggtat cccccaagcc ctgcagagcg ccagccatgt
     3781 tcaaagaaac cgcaaggcct ggctggttga aagcatcgat ccgtaaggct gtgacgactt
     3841 gatggactgc ttctaggtct ttggtaaata gcagagcaaa acttggagct ccgaaaaata
     3901 agatggtacc gagcaggaac atgatggcaa caccataggc agagctgaga aatgcgacac
     3961 gtctaactgt gaggatatcc ccctttacca aggcttgacc gatcaaaacc gccgccgctg
     4021 tcgcaagacc ataggctggc atataggtaa aactctcgat attgcctgca atcatgtgag
     4081 aagcatagac agtcgtcccc agccccacaa tcaaactgaa atagaccacc tgtcccaacc
     4141 gcataaccaa gcgttcggct gctgcaggta aggtcaaatc caccatttcc ttaggacttc
     4201 ccaagtggaa caattgtttg agatcaactg ccaagtcagt ctgctgtacc tttcggtaaa
     4261 gcaagacagt accaagcaaa cgagctaaga cggttcccaa ggctgtccct acaatcccca
     4321 aacctgacca agagccaata ccaaaaatca agaaatagtc caagaccaca ttaaaaccat
     4381 tggtcagtaa gctcattttc ataggggata ctgtatcacc agatgcccgc aggattgttc
     4441 ccaaaatagt catcaaggcc tgaaaaatcg tcaaaccacc aacccagtaa aaaaatgcct
     4501 gggcaccagc caaactctcc gcatctgctc ccattaagac cagcatctgc cgcccgaaaa
     4561 caagggatag taaaccaaat agcacaccta cacctactga aagcactaag gcttgacgga
     4621 catgaaaggt aagagattcc ctatctcctg cgccaataga ccgagcaatc aaagctgttg
     4681 ccccaactcc caaagccata tagaccgcca aatagacgtt caaaatcgta ttggcaagac
     4741 caactgccgt caccgctacc agccccagct gtgcaatcaa gagggtatcc acaaaaccaa
     4801 ctaaggtctg aaagatattt tccaccgtag caggcaagga caaacgaata atttcttttc
     4861 ctaaactttc ttgcttcatt ttcctctcca cgattttatt tagaataatt ataaataaaa
     4921 ctcggtgaaa aagaaagaaa acagggagtg gggaagaact cgataagtca aaaaggagtt
     4981 catctctcca cccccgcata gctccaaagt ttcagggaac ctttggaggt tagagataag
     5041 gcgaacaaag ttcgcatcag tcgtataggt cagatttgga gtggaaaaca cgaattgctg
     5101 agatttgctt cgcaaacctt atctccaacc tttttaccta gcttttctgc ttcaaggctc
     5161 gggctgaaac agttcccaga actgcttcac tccactggac tgttaaagcc aatcaaccac
     5221 tgcgctgaga tgttgacacg aactctgaga agtacaccga taccgagcct agttacctag
     5281 aacttcattt tttatagatt tctttccggt gcccgatttc aagggccagg atgaccattt
     5341 tgttatcaat aatatcacaa attacacggt aattccccac tctataccgc cacagcccct
     5401 tatagtctcc taccaaggcc ttcccaaatt gtcttggatt ctccaaaccg tccaaacgtt
     5461 ttttcaaatc cttagcaagc atcatcccca catgcctgtc catttttttc aactgcttca
     5521 aagcatcatc gctgagaaca agcttataag ccatcttcta actccgcaag aacatcatca
     5581 atcgagtgtg taacaggatt ttcttggaat ttcttcaaag ccttgatacc aatttcatag
     5641 tccaactggt cttctatctg ttcagcaagt gcagttttga atagctcaga caggcttttt
     5701 cctgtatgaa tagcatagct tctgaaaatt tcttcttctg catcgtttaa tcgcaaggat
     5761 acaattccca tcattccacc tactttcttc ttgtaataca ttgtaaaaca attcgttcaa
     5821 aatgtcaatt gacaccagaa aaacagacca cgtttgtagt ctgtcagtga atatttattt
     5881 cgcagtcttt cagtttactt ttagtactag gcaacgagct gcagactgta ctaaggtacg
     5941 gcaaaacgag ttcaaataat gatgagatta tttgaacttg gaaataagga aacggagttt
     6001 cctcatacgt cgacgtaata aaggaaaaac tgaacgactg tattacggat gcaagcggtg
     6061 caacatacgt gggaacggaa tggcttcacg gatgtgtttt gtacctgcaa caaaagtcac
     6121 catacgctcg ataccgatac cgaaaccacc gtgtggtact gaaccgtact tacgtaggtc
     6181 aaggtagaat tcgtattctg acttatccat gccgagctcg tccatcttgg ctaccaaggc
     6241 atcgtaattg tcctcacgca tagaaccacc gatgatttca ccgtagcctt ctggtgccaa
     6301 gaggtccgca caaagcacgc gctctggatt acctggaact ggcttcatgt agaaggcctt
     6361 gaagcttgct ggatagttaa caacgaaagt cggtacacca aagtagtttg aaatccaagt
     6421 ttcatgtggc gatccaaagt catcaccatg ctcgatgtgt tcgtagtctg tatcttcatc
     6481 agcttcatgc tcttgaagaa gggtaatggc atcatcataa gccacacgtt tgaatggctc
     6541 cgcaatgtag cgtttgagag catccacatc acgctcaaga gtttccaagg cttgtggagc
     6601 acggtcgata acaccttgaa tcaaggcttt gacataagct tcttgcaaat caagtgactc
     6661 ttcatggctg aggaatgaat actcggcatc catcatccag aactccgtca agtgacggcg
     6721 agtttttgat ttttccgcac ggaaaacagg tccgaagtcg aaaacacgac caagggccat
     6781 ggcaccagct tccaggtaca gctgaccaga ttggctgagg taggcaggtt gaccaaagta
     6841 gtcagtttcg aacaactctg tcgaatcttc tgcagcgttt ccagacaaga ttgggctatc
     6901 aaacttgata aagccattct tttcaaagaa ttcataagtc gcatagatga tggcgttacg
     6961 gatttgctgg atagcgacct gtttacgaga acgcaaccag aggtgacggt tgtccataag
     7021 gaagtccgta ccgtgttctt ttggtgtgat tgggtaatct tgtgactcac cgattacttc
     7081 aaggtctgtc acatccaact cataaccaaa cttagaacgc tcatcttcct tgacaattcc
     7141 tgtcacatag acagacgttt cttggctgag gcgttttgct acatcgaact tctcagtccc
     7201 agcttcttca ccgaattttt cgatgaagtt tggttttaag gcaactgctt ggaagaaggc
     7261 tgttccgtca cgcaattgca ggaaggctaa cttgcccttg cctgatttgt tggcaaccca
     7321 ggcaccaata gtcacttctt gaccgacata atctttgact tggttaatgg taatcaattt
     7381 tctagacatt atttttcctc tttttatttt ttcataaatg tatgtagtcg agccactgct
     7441 tctttgagcg tgtccatatc tgtcgcatag ctgaggcgga tattctcagg agctccaaaa
     7501 ccagcacctg tcaccattgc aactcccact tcttccaaaa tggctgtggt aaattcagtt
     7561 acatctgtgt agcctttcat ttccatagct tttttaacat tcgggaagag atagaaggcc
     7621 ccttctggct taatcacttc aaaacctggt acttctgcca gcaagggata aatggtattg
     7681 agtcgctctt caaaggcctg acgcatggtt tctactgtat cttgcggacc tgtaaaggct
     7741 tcaatggccg catactgagc agcagtcgtt aaattcgaag tcgtttgtcc gacaatctta
     7801 ctcatggccg caatgatttc aggattacca acggcaaaac caacccgcca acctgtcata
     7861 gcataggcct ttgccacccc attgacaaca atagtctgct gacgaatgct ttctgaaatg
     7921 cttgaaatcg gtgtgaagcc atttccatta tagaccaaac gaccataaat atcgtctgcc
     7981 aagatgagaa tatcgtgctc tacagcccag tttccaattg cttccaattc ctcacgactg
     8041 taaatcatcc cagtcggatt agaaggagta ttgagtagga atactttggt cttatctgtc
     8101 cgagctgctt ccagctggtc aaccgttacc ttgaaattat gctcttccct tgcagtgaca
     8161 aagacaggca ccccatcatt catcttgatt tgatctgcat aagaaaccca gtaaggtgtc
     8221 ggaataatca cctcatcacc aggattgatg accgccgcaa agaaagcata aagaacaaac
     8281 ttagcacctg tcgcaagaac gacttgattg cgttcgatag agtagccata gaaattctca
     8341 aagtaggtat tgactgcgtc cttcaactca ggatgccctg aagctaccgt ataaaaactc
     8401 gccttaccat cttcgataga cttaatagca gcttcttgaa tatttttcgg tgtgacgaag
     8461 tctggctcac ccaaggtcag ctctaaaata tcacgtcctt ccgccttcaa ggcttttgca
     8521 cgcgcacctg ctgccagagt tacactttct tccatctcta agactcgtct tgataacttg
     8581 ctcataagcc ctcccgtttg ataaagttcc ctgtttcaaa ttcaactaaa tagtaggcag
     8641 tacctgactt gacttcccaa ataggtttgc catcccttaa acctagaata gttcgctcta
     8701 cttggcttgc accgtttttc ttggcaatag cttgcgcttc ttcttgcgaa attccctgaa
     8761 ctaaaggata gacatatact gtgctagaag cctctggaat caaaactgca acctcttctc
     8821 cctgactggt gacaccaaat acacaaaaat aggtttcagt accattgtag atagaaaaat
     8881 cattgacagt ttgcaaatca gcatattctg tagctactgc aactgcatga ctacgcacat
     8941 tgttgaaagg ggcagcagca atatcccaaa tagtaaaaat tgaaaaagtc acaacactga
     9001 gaaggataaa agagcccaac actaactgac cagtcttgga agccatccac tccaacagtt
     9061 gaaaaatctt tttttccata gtagtcccat tatactacaa attaaagcat atttccatt
//
LOCUS       DBNFEG010000082         8908 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.082, whole genome shotgun sequence.
ACCESSION   DBNFEG010000082 DBNFEG010000000
VERSION     DBNFEG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8908
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.082"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            205..1692
                     /locus_tag="AC1PNG_001270"
     CDS             205..1692
                     /locus_tag="AC1PNG_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HIP6701777.1"
                     /translation="MKKIAFDSTKYLNLQRDHILERIAQFEGKLYMEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKDQVEIVIAINASNIEHSKARGDLGISYDQEVFRLIDTF
                     NDIDIYVGSVVITQYRNQPAADAFRKQLEKHGIKSYLHYPIKGYPSDIDHIISPEGMG
                     KNDYIETSRNLVVVTAPGPGSGKLATCISQLYHDQLHGVTSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDLNMIDPFHLQTYGKTAVNYNRDIEVFPVLNRTFERILNKSPYA
                     SPTDMGVNMVGYSIVDEEAAIEASKQEIIRRYYQTLVDFKAERVSEQAVKKIELLMNE
                     VGVTPADRKVVIAAREKAELTTSPALAIQLPTGEIVTGKTSDLLKPTATVLLNAIKQI
                     ANIDDETLLIEPNYIRPIQELKADYLDKTNTRLDASEILNALAITAQDSPLAARAMKE
                     LGQLNGSEAHSTVILSDEDKSVLRKLGINLTFDPIYQHNKFYQKN"
     gene            complement(1774..3507)
                     /gene="ptsP"
                     /locus_tag="AC1PNG_001271"
     CDS             complement(1774..3507)
                     /gene="ptsP"
                     /locus_tag="AC1PNG_001271"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIP6701778.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVEDTNAEEARL
                     DAALEASQNELSVIRENAVASLGEEAAAVFDAHLMVLADPEMIGQIKETIRAKKTNAE
                     TGLKEVTDMFIAIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVRLPNPATIDEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     VLAVNGITGDVVINPSEEVIAEFKVAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPHEMNPFLGFRALRISISETGNQMFRTQLRALLRSSVHGKLRI
                     MFPMVALLTEFRAAKAILEEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYAHRALTECATAEEV
                     LELQKEYVDFD"
     gene            complement(3512..3775)
                     /locus_tag="AC1PNG_001272"
     CDS             complement(3512..3775)
                     /locus_tag="AC1PNG_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HIP6701779.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLNYKEKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAITETMEKEGLA"
     gene            complement(3931..5112)
                     /gene="icd"
                     /locus_tag="AC1PNG_001273"
     CDS             complement(3931..5112)
                     /gene="icd"
                     /locus_tag="AC1PNG_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019780541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIP6701780.1"
                     /translation="MAEKILMREGRLIVPDCPVIPYIEGDGIGRDIWSVSQKVIDAAV
                     KKAYAGQRSIQWREVLAGEKAYELTGTSLPNETLEIIREHLVAIKGPLGTPIGKGHRS
                     LNVALRQELDLYACVRPIRYFKGVPSPLKEPEKTDITIFRENTEDIYAGIEWEAGSDE
                     VAKVISFLQEEMGVVNIRFPESSSIGIKPISEEGSKRLIRSAIEYALEHQLKIVTLVH
                     KGNIQKFTEGGFRKWGYELAKEEFATELAEGTLVVNDIIADNFLQQILLAPENFQVVA
                     LTNLNGDYASDALAAQVGGIGISPGANINYQTGHAIFEATHGTAPDIAGKDLANPCSL
                     LLSACMMLEYIGWKEAATLIIQALEDAFINKNVTLDFAKILDVAHQSTSQFSEVLIDS
                     I"
     gene            complement(5115..6233)
                     /locus_tag="AC1PNG_001274"
     CDS             complement(5115..6233)
                     /locus_tag="AC1PNG_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIP6701781.1"
                     /translation="MTETNGLKDAIACDTRISAIENDRLSYAGYDIAELMENNASFEE
                     VIYLLWNLHLPTQIELNYFEKSLRKEYAISDAVEQCILIQSRRHLHPMSVLRSTVSLL
                     GVYNVHAEENSLEALYEQSIQIMGKVPTIIATFARLRRGLVPIEPRSDLGFAANFLYM
                     LNGEEPSDLQIKAFNKALVLHADHELNASTFAARVTASTLTDLYSCVTTAIGTLKGSL
                     HGGANERVFDMLLAIRESGDTERYLQKKLDSKEKIMGFGHRVYKTVDPRQEYLKEMAR
                     DLTFGTDDEAFYRLSEEVESFIKNKKGLIPNVDFYSATVYHVLGIDSDIFTLIFAMSR
                     MAGWIAHVQEQRKNNKLIRPRSSYLGGRNLVYIPIGKR"
     gene            complement(6230..8899)
                     /gene="acnA"
                     /locus_tag="AC1PNG_001275"
     CDS             complement(6230..8899)
                     /gene="acnA"
                     /locus_tag="AC1PNG_001275"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIP6701782.1"
                     /translation="MEKSHRKRLIHKGEEYSYYALDSISMEEKVDIHSLPYTIRILLE
                     SLLRKEDGVDVTKNHIMELLHYQAASPKGEIPFKPSRVILQDFTGVPVVVDLASMRDA
                     VVKAGGNPELINPEIPVDLVIDHSVQVDFFGTEDALEKNIALEFERNNERYEFLKWAE
                     NSFENYRAVPPATGIIHQVNIEFLSDVIINKDGLLYPDSMFGTDSHTTMINGIGVLGW
                     GVGGIEAEAAMLGEASYFPVPEVIGVRLAGQLPKVATATDLALKVTQLLRQENVVGKF
                     VEFFGPGLSSLALADRATVSNMAPEYGATCGYFPIDGETLHYMRLTNRSEEHVELTEA
                     YTKANYLFYDAERFPSYSKVLELDLSTVVPSISGPKRPQDLIELTDAKAEFQASLIRE
                     VGVRGFGLEEAELDKTATVKYVEGDEQIQTGHVAIAAITSCTNTSNPYVLLAAGLLAK
                     NAVEKGLAVSKTVKTSLAPGSKVVTGYLKKSGLQTYLDALGFNLVGYGCTTCIGNSGD
                     LRPEVAEAIKEEDLLVSAVLSGNRNFEGRINPLVKANFLASPPLVVAYAIVGNMNVDL
                     TRDPLGYDEKQQAVYLADIMPSREEVDDYIERYVTRDLYKEEYQQVFTDSQAWNAIET
                     KTDKNYNWNSSSTYIQNPPYFDNMQVDLSIKPLENLSVLAKFGDSVTTDHISPAGNIA
                     RLSPAARYLEENGIVYKDFNSYGSRRGNHEVMMRGTFANIRIKNELAAGKIGGWTRVG
                     DEILPIYDAAMKYKAAGIGSIVIAGKDYGMGSSRDWAAKGSSLLGVKAVLAESFERIH
                     RSNLVMMGVLPLQFLEGQSAESLGLTGHESYTIDLPEDVGVGQIVTVHAQIDDVTKEF
                     QALVRFDAEADIRYYRHGGILPMVVRKKLGGKV"
BASE COUNT         2617 a         2140 c         1576 g         2575 t
ORIGIN      
        1 aatatttaac tttataaaaa ttaaatattt tttaaacact tgtttaactt ttcttctgtc
       61 tgactttttc ttgacaagat tagcacggtt tgttatagta ttgttcgggc acctcttttg
      121 agaggtcgga gaaaaggctc cctagtttta gggagctatt tttgtttttc ccagagatta
      181 atacacattt ggaggactta tttgatgaag aaaatcgcat ttgattcaac taaatacttg
      241 aatttgcaac gtgaccatat tttagaacga attgctcagt ttgaaggcaa gctctatatg
      301 gaatttggcg gaaaaatgct ggaagatttc cacgcagccc gtgttttacc tggctatgaa
      361 ccagataaca aaatcaaact cctccaagag ttaaaagacc aagtagaaat cgtcatcgcc
      421 atcaatgcaa gcaacatcga acattctaag gcacgtggtg acttgggtat ttcttatgac
      481 caagaagtct tccgcttgat tgacaccttc aatgatattg atatttatgt tggttctgtg
      541 gtcattaccc agtaccgcaa tcaaccagcc gcagacgcct tccgcaagca gttggaaaaa
      601 cacggcatca agtcctatct ccactaccca atcaagggct atccttctga tatcgaccac
      661 atcatttcac cagaaggtat gggcaaaaat gactacattg aaactagtcg caatctcgtc
      721 gttgtcaccg cacctggacc tggttcaggt aaattggcga cctgtatctc ccaactttac
      781 cacgaccaat tgcatggagt aacatcaggc tatgctaagt ttgaaacctt cccagtgtgg
      841 aacttaccac tccaccatcc cgttaacttg gcctatgaag cagcaactgc tgacctggac
      901 gacctcaaca tgatcgaccc cttccacctg caaacttacg gcaaaactgc ggtcaactac
      961 aaccgtgata tcgaggtctt ccctgtcctc aaccgtacct ttgaacgtat tttgaacaaa
     1021 tcaccatacg cctcaccgac agacatgggc gttaacatgg tgggctattc catcgtggac
     1081 gaagaggctg cgattgaagc atccaagcaa gaaatcatcc gccgctacta ccagaccttg
     1141 gttgatttca aggcagagcg ggtgagcgag caagcagtta agaagattga actcctcatg
     1201 aatgaagtcg gtgtgacgcc agcagatcgt aaggttgtta tcgctgcgcg cgaaaaagca
     1261 gagctaacaa ctagccctgc ccttgctatt cagttgccaa ctggagagat tgtaacaggt
     1321 aagacatccg acctcctcaa gccaactgca actgtccttc tcaacgccat caagcaaatt
     1381 gctaacatcg atgatgaaac acttctcatc gaaccaaact acatccgtcc aattcaagaa
     1441 ttgaaggcag attatctgga taagacaaat acacgactcg atgcaagcga aattcttaac
     1501 gcccttgcca tcactgcaca agatagccct cttgctgcac gtgccatgaa agaattaggc
     1561 caattaaatg gtagcgaagc ccactcgact gttatcctat ccgatgaaga caagagtgtc
     1621 cttcgtaaat taggcatcaa tcttaccttt gaccctatct accaacacaa taagttttat
     1681 cagaaaaact aagcacacaa aaagacttgc tccgacaagt ctttttgtga acagtaaaaa
     1741 agcaaggatt caattccctg cttttttcaa cgattaatcg aaatcaacat actctttttg
     1801 aagttcaaga acttcttctg ccgttgcaca ttctgtcaag gcacggtgag cgtattcttc
     1861 catcttagct gtatcaagtt tcttcataag gctacgtgta cgaagtacag atgttgcact
     1921 catagagaac tcatccaagc ccatacctac aagaagtgga acagcttgtt gatcaccagc
     1981 catctcacca cacataccag cccatttacc ttctgcgtgt gcagccttga taacattgtt
     2041 gatcaagcga aggattgatg ggttatatgg ttggtagagg tatgaaactt gttcattcat
     2101 acggtctgca gccattgtat actggataag gtcgttcgta ccgattgaga agaagtcaac
     2161 ttctttggca aattggtctg caagcattgc tgctgcaggg atttcaatca tgataccaac
     2221 ttggatatca tccgcaactg ctacaccttc agccaacaag tttgcttttt cttcttcaag
     2281 gattgctttt gctgcacgga attcagtcaa caaggcaacc attgggaaca tgatacgaag
     2341 tttaccatgt actgatgaac gaagaagggc acgcaactga gtgcggaaca tttggttacc
     2401 agtctcagag atagagatac gaagggcgcg gaaacctagg aatgggttca tttcgtgtgg
     2461 aagatcgaag taaggaagtt ccttatcacc accaatatcc attgtacgaa ctacgactgg
     2521 cttaccattc atgccttcaa gaacagcctt gtaagcttcg tattgctcgt cttcagttgg
     2581 gaagtcttgt gaatccatgt acaagaattc tgtacggtaa agaccaacag cttctgcacc
     2641 gttatcattt acaccttcaa cgtcttttgg tgtaccgata tttgctgcca attcaaagtg
     2701 tttgccgtca gctgtaactg tttgagcatc tttcaaaaga gcccattcag ctttttgttt
     2761 agcataggct tcaccagcta ctttaaattc tgcaatcact tcttcagaag ggttaatcac
     2821 tacatcacct gtgataccgt taacagccaa tacatcgccg tctttaacga tttcagtaat
     2881 gttatttgta cccaaaacag cagcaatttc aagtgtacga gccatgatag ctgagtgact
     2941 tgtacgacca ccaatgttcg ttacgaaagc ttttacaaac tgtttgttta attgcgctgt
     3001 atctgaaggt gtcaagtcat gtgcgataac gattgactct tcgtcgatag ttgctgggtt
     3061 tggcaagcga acaccaagca aatgtgccaa tacacgtttg gctacgtcac gaatatctgc
     3121 ggcacgctct tgcatgtatg ggttatcttc cataccttca aagatagcga taaacatatc
     3181 cgtaacttct ttcaaaccag tctcagcgtt tgttttcttc gcacgaattg tttccttgat
     3241 ttgaccaatc atttctgggt cagcaagaac catcaagtgt gcatcaaata cggctgcggc
     3301 ctcttcacct aggctagcta ctgcgttctc acgaataaca gaaagctcgt tttgagatgc
     3361 ttctagagca gcatccaaac gagcttcctc tgcatttgta tcttcaactg taacagtttc
     3421 gaaggacaaa tctggctgaa cgagtagata tgccttagca acagcaacac catctgatgc
     3481 tgcgattcct ttaagcattt ctgtcatatt cttatgccaa tccttctttt tccattgttt
     3541 cagtgatagc tgcgattgcg tcgtcagcat cagcaccttc agcagagatt gttacgtcag
     3601 cgccttgacc aacaccaaga ctcataacac ccatgataga tttaaggtta acagactttt
     3661 ctttgtagtt caaagtgatg tctgaagcaa atttactagc tgtttgcaca agcaaagttg
     3721 ctggacgtgc gtggatacct gtttctgcca caatgtggaa gtcttttgaa gccatatttg
     3781 gattctcctt tatttttaca aaattgtgag ttatttgtga taacccttac aaaggacatt
     3841 ataccacttt ttgaaatcat tttcaagatt tttttacctt tctttcatgt ttccgtcata
     3901 aaaagatagg ttcattttac tttttcttca ttaaatagaa tctattagaa cttctgaaaa
     3961 ttgactagta gattgatgag caacgtccaa aattttagca aaatcaagcg taacattttt
     4021 atttataaaa gcgtcctcca aggcctgaat aattaaagta gccgcttctt tccagcctat
     4081 atattctaac atcatacaag ccgaaagcaa gagggaacat ggattagcca aatcctttcc
     4141 tgcaatatca ggagcagttc catgtgtcgc ttcaaagata gcatgacctg tctgataatt
     4201 aatattagca cccggtgaga tgcctattcc tccgacctga gccgcaagag catctgatgc
     4261 ataatctcca tttagatttg taagagcaac cacttggaag ttttccggcg ccaataaaat
     4321 ctgctgcaga aagttatccg caataatgtc atttactacc aaagtcccct ctgcaagttc
     4381 tgtagcaaat tcttctttcg ccaattcata accccacttg cggaaaccgc cttctgtaaa
     4441 cttttgaata tttcccttat gaactaaggt cactatcttc aattgatgtt ctagggcata
     4501 ttctattgca gagcgaatga ggcgtttact cccttcttcc gaaatcggtt tgataccaat
     4561 acttgaagat tctggaaaac ggatatttac cactcccatt tcttcttgca gaaaggaaat
     4621 gactttggca acctcatctg aaccagcctc ccattcaata cctgcataaa tatcttctgt
     4681 attttcacga aaaatagtaa tatccgtctt ttcaggctct ttcagcggac ttggcactcc
     4741 cttaaaatag cgaatcgggc gcacacaggc atacaaatct aattcttgac gaagtgcaac
     4801 attaagagaa cgatgacctt tcccaatcgg cgttcccaag ggacctttga ttgcaactaa
     4861 atgctcacgg attatttcca gtgtctcatt cggcaaagaa gttcccgtta attcataagc
     4921 cttttctcct gccaaaacct ccctccactg aatagaacgt tgacctgcat aggctttttt
     4981 cactgcagca tcaataactt tttgactgac tgaccaaata tccctgccaa taccatctcc
     5041 ttcaatatag ggaataacgg gacaatctgg gacaattaac cgtccctctc tcattaggat
     5101 tttttctgcc atatttacct ctttccaatc gggatgtaaa ccaaattcct accacctaag
     5161 taggaagagc gcggacgaat caatttatta tttttccgtt gttcctggac atgtgcaatc
     5221 caccccgcca tacggctcat tgcaaaaatc aatgtaaaaa tatcactatc aatacctagc
     5281 acatggtaaa ctgtcgccga ataaaaatct acgtttggaa ttaaaccttt tttgttttta
     5341 ataaagcttt ctacttcctc tgacaatcta taaaacgctt catcgtctgt tccgaatgtc
     5401 aaatcacgcg ccatctcctt caaatactct tggcgaggat caaccgtttt atagactcga
     5461 tggccaaatc ccataatttt ttccttcgaa tccagcttct tctgcaaata tctctcggta
     5521 tcaccactct cgcgaatagc cagcaacatg tcaaacactc gttcatttgc accaccatgc
     5581 aatgagcctt tcagagtccc aattgccgtg gtaacacaag aatataaatc cgttaacgtt
     5641 gatgccgtga cacgcgcagc aaaagtagat gcattcaatt catgatcagc atgaagcacc
     5701 aaagctttat taaacgcttt gatttgtaaa tcactcggct cctcaccatt cagcatataa
     5761 agaaaattcg ccgcaaaacc cagatcagac cgcggttcaa ttggaaccaa tccgcgtctc
     5821 aaacgagcaa atgttgcaat aatcgtcgga accttgccca taatctgaat agattgttca
     5881 tacaaagctt ctaacgaatt ctcttcagca tgtacattat atactccaag gagagatact
     5941 gtcgaacgca acacgctcat aggatgcaaa tgtcgccgtg attgaatcag aatacattgc
     6001 tctaccgcat ctgaaatcgc atattctttt cgcagacttt tctcaaaata atttaattca
     6061 atctgagttg gcaaatgcag gttccacaga agatagatga cttcctcaaa agaagcgtta
     6121 ttctccatca attcagcaat gtcataccct gcataggaca agcggtcatt ttcgattgca
     6181 ctgattcgcg tatcacaggc aatcgcatct tttaacccat tcgtttctgt catacctttc
     6241 ctcctagttt ctttcttact accataggta aaatacctcc gtggcgataa taacgaatat
     6301 ccgcctctgc atcaaaccgc accagagctt gaaactcctt tgtaacatcg tctatttgcg
     6361 catggactgt tacaatttga ccaacaccga catcttctgg caagtcgatt gtatagcttt
     6421 catgtccagt caagccaaga ctttctgccg actgaccttc taaaaattgt aaaggaagaa
     6481 cacccatcat cactaaattt gaacggtgaa tccgctcaaa tgactcagca agaacagcct
     6541 tcacaccaag taaacttgaa cctttggctg cccaatcacg gcttgacccc atcccatagt
     6601 ctttaccagc aataacaata ctaccgatac cagcggcctt atatttcatg gctgcatcat
     6661 agataggaag aatctcatcg ccaactcttg tccaaccacc aatttttcca gctgccagct
     6721 cattctttat acgaatattt gcaaaagtcc cacgcatcat gacttcatga tttccacgac
     6781 gacttccata agaattaaaa tccttataaa caatgccatt ctcttcgaga taacgagctg
     6841 caggactcaa tcgtgcaata ttccctgccg gggaaatatg atctgtcgtt acagaatcgc
     6901 caaatttagc caaaacagat aaattttcca gtggttttat ggataaatct acctgcatgt
     6961 tgtcaaaata gggcggattt tgaatatagg tagaagatga attccaattg taattcttat
     7021 ctgtttttgt ttcaatcgcg ttccaagcct gactatctgt aaatacctgt tggtattctt
     7081 ccttataaag gtcacgagtc acataacgct caatgtagtc atctacctcc tcacgacttg
     7141 gcataatgtc cgcaaggtag acagcttgtt gcttctcatc ataccccagc ggatcccttg
     7201 ttaaatctac attcatattt cccacaatag cataggcaac aacaagcggt gggctcgcta
     7261 agaaattagc tttaaccaat ggattaatcc gtccttcaaa gttacgattt ccagataata
     7321 ccgcagaaac caataaatct tcttccttaa tcgcctctgc tacttcagga cgcagatcac
     7381 ctgagttacc gatacaagtt gtacaaccat aaccaaccaa attgaaccct aaggcatcta
     7441 gataggtctg caaacctgat tttttcaaat agccagtcac tacttttgag cctggtgcca
     7501 atgacgtctt gaccgtctta gaaactgcca aacctttttc taccgcattt tttgccagta
     7561 atcctgccgc caaaaggaca tacggattgg aggtatttgt acacgaggta atcgccgcaa
     7621 tagcaacatg acctgtttga atttgctcat ctccttcaac atacttaacc gtggctgttt
     7681 tatctaactc tgcttcctcc aaaccaaatc cacgcacacc tacttcacgt attaaactag
     7741 cctgaaactc tgcttttgca tctgttaatt ctatcaaatc ctgtggtctt ttaggaccag
     7801 aaatagatgg gactactgtc gataaatcca attctaaaac cttgctataa gacggaaaac
     7861 gttcagcatc atagaataaa taattagcct tggtataggc ttctgtcaac tccacgtgct
     7921 cctccgaacg gttggtcaat ctcatataat gtaaagtttc tccatcgatc gggaaatagc
     7981 cacaagtcgc accgtattct ggagccatat ttgaaacagt tgctcgatcc gctaatgcaa
     8041 gagaagataa acctggtcca aaaaattcga caaacttccc aactacattt tcctgacgta
     8101 gaagttgagt gaccttaagc gccaaatccg ttgcagtggc aaccttaggc aattgcccag
     8161 ctaggcgaac accaatcacc tcaggaacgg gaaaataaga tgcttcaccc aacattgcag
     8221 cttccgcctc aataccacct acaccccaac caagtacacc aataccgtta atcatggtcg
     8281 tatgactgtc tgtaccaaac atagaatctg gataaagcaa accatccttg ttgataataa
     8341 catcgcttag aaattcaatg tttacctgat ggataattcc tgtcgctgga ggcacagcgc
     8401 gataattttc aaaggaattc tctgcccatt tcagaaattc atagcgttca ttatttcttt
     8461 cgaactctaa ggcaatattt ttctccagag catcttcagt cccaaaaaaa tcaacttgaa
     8521 cagagtggtc gatgactaaa tccactggaa tttccgggtt aatcaattca gggtttccac
     8581 ctgctttcac caccgcatca cgcatggagg ctaaatctac cacaacagga acacctgtaa
     8641 aatcttgcaa aataacacga ctaggtttaa aggggatttc ccctttagga gatgcagcct
     8701 gataatgaag caactccatt atgtgatttt ttgtaacatc cacaccatct tcttttctta
     8761 aaagactctc taataaaata cgaatagtat agggaagtga atgaatatcc actttttctt
     8821 ccattgatat cgaatccaat gcataatatg aatattcttc ccctttatga atcaagcgtt
     8881 ttctatgtga tttttccata ttttataa
//
LOCUS       DBNFEG010000083         8757 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.083, whole genome shotgun sequence.
ACCESSION   DBNFEG010000083 DBNFEG010000000
VERSION     DBNFEG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8757
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.083"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(139..1161)
                     /gene="ilvC"
                     /locus_tag="AC1PNG_001276"
     CDS             complement(139..1161)
                     /gene="ilvC"
                     /locus_tag="AC1PNG_001276"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIP6701783.1"
                     /translation="MTVTMQYEKDVTVAALDGKRIAVIGYGSQGHAHAQNLRDTGHDV
                     IIGVRAGKSFDKAKEDGFETFEVAEAAKQADVIMILAPDEIQADLYNEEIAPNLEAGN
                     ALGFAHGFNVHFEFIKVPADVDVFMCAPKGPGHLVRRTFEEGFGVPALYAVYQDATGN
                     AKHIAMDWAKGVGSARVGLLETTFKEETEEDLFGEQAVLCGGLTALMQAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLVYEGGFKKMRQSISNTAEFGDYVSGPRVITDQVKENMK
                     AVLADIQSGKFANDFVNDYKAGRPRMEAYRKEAENLEIEKVGAELRKAMPFVGRNDDD
                     AFKIYN"
     gene            complement(1293..1769)
                     /gene="ilvN"
                     /locus_tag="AC1PNG_001277"
     CDS             complement(1293..1769)
                     /gene="ilvN"
                     /locus_tag="AC1PNG_001277"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIP6701784.1"
                     /translation="MRRMLTAKLRNSSGVLNRFTGVLSRRQINIESISVGPTEVDGIS
                     RVTVIVDVVSHDEVEQIIKQLNRLIDVVRVRDLTDIPHLEREVILVKISAPPTKRAEI
                     LAIIQPFRANVVDVAPHSITIQMTGDGDKIDALLRVIQPYGIKNIARTGATGFSRD"
     gene            complement(1762..3465)
                     /locus_tag="AC1PNG_001278"
     CDS             complement(1762..3465)
                     /locus_tag="AC1PNG_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HIP6701785.1"
                     /translation="MQEIQLDQPRSGSYLILDTLHQLGVDLVFGYPGGAVLPLYDAIY
                     QYEGIQHILARHEQGAVHEAEGYAKSSGKVGVAIVTSGPGATNAITGIADAMGDSVPL
                     LVFTGQVATRGIGKDAFQEADVLGMTMPITKYNYQIRDTADIPRVITEALHIATTGRP
                     GPVVIDVPKDIQERVVDFYHDPTLHLPSYQPTIEPNGLQVKKILKQLSQAQKPVILAG
                     GGVNYADANKELVAFAERYRIPVVSTLLGLGAMPIEHELSLAMGGMHGSYAANMAMDQ
                     ADYIINIGARFDDRLTGNPTTYAPNATVAHIDIDPAEIGKVVKTAIPVVGDAKATLKA
                     LLALETVETGYADWTAQVLENKRRAPFWYDEDEKVIKPQVAIELIGQLTQGDAIVVTD
                     VGQHQMWAAQFYPYKHARQLVTSGGMGTMGFGIPAAIGAKLANPDKEVIIFVGDGGFQ
                     MTNQELAILNGYGVPIKVVLINNHSLGMVRQWQESFYEKRRSQSVFDDEPNFQLLAEA
                     YGISHYSFDNPATLSEDMKVILEDKPMLIEVHISNREHVQPMVPAGKSNAEMLGVKFN
                     A"
     gene            3690..4856
                     /locus_tag="AC1PNG_001279"
     CDS             3690..4856
                     /locus_tag="AC1PNG_001279"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIP6701786.1"
                     /translation="MYYVVIPAYQPDEKLIQLLEIMKNRLNCQVIVVDDGSTGASKNI
                     LEIAKTYAIVLHHDVNKGKGQALRTAFSFIQDLRKPGVIVTADADGQHTVNDIDRVAR
                     AAMNLPSRLILGVRQFTKDIPLRSRFGNKLTRVLFRLQTGVNVSDTQTGLRGFHTDLI
                     PFMLDIEGDRYEYEMNMLTQASQRYKITEVPIQTIYIDDNASSHFRPIKDGLLIYKNL
                     FKFALASFGGFLVDYGIYALALLLLTSLPTALRLLVANTIARICSATCNFALNKKLVF
                     NNEESIARTGAGYFTLALVLFLCDTALLYLFHQILGLNLYIVKLAVDIFLFLASWFVQ
                     KNIIFKERKSFSHEIAKKNLCLRSHLRNAFDRRLYLLHVKNLRAFRGYHNGCCK"
     gene            4774..5662
                     /locus_tag="AC1PNG_001280"
                     /pseudo
     CDS             4774..5662
                     /locus_tag="AC1PNG_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445339.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
     gene            6215..7930
                     /gene="ilvD"
                     /locus_tag="AC1PNG_001281"
     CDS             6215..7930
                     /gene="ilvD"
                     /locus_tag="AC1PNG_001281"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIP6701787.1"
                     /translation="MTDTNKLKDFRHRSSVYDSMVKSPNRAMLRATGMTDDSFEKPIV
                     GVISTWAENTPCNIHLHDFGKLAKEGVKDAGAWPVQYGTITVADGIAMGTPGMRFSLT
                     SRDIIADSIEAAMGGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAVFAYGGTIAPGNL
                     NGKDIDLVSVFEGIGKWNNGDLTAEEVRQIECNACPGPGGCGGMYTANTMATAIEVMG
                     MSIPGSSSHPAESPEKKADIEEAGRAVVRMLELGIKPSDIMTREAFEDAITVTMALGG
                     STNATLHLLAIAHAANVELTLEDFNDFQERVPHLADLKPSGKYVFQDLYNVGGVPAVM
                     KYLLKNGFLHGDRITCTGKTVAENLENFADLTPGQDVIMPLENPKRADGPLIILKGNL
                     APEGAVAKVSGVKVRNHTGPAKVFDSEEEAIEAVLTDEIVDGDVVVVRYVGPKGGPGM
                     PEMLSLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDLV
                     TVDQDTKEITMHVSDQEIEDRKKTTVIPPLYSRGVLGKYAHTVSSASKGAVTDFWRPE
                     RTGKK"
     gene            7981..8367
                     /locus_tag="AC1PNG_001282"
     CDS             7981..8367
                     /locus_tag="AC1PNG_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6176 family protein"
                     /protein_id="HIP6701788.1"
                     /translation="MKLNVELSRFRVKEGKTVQVDEWMAFLNEHMEDTLLTLEGEKMY
                     VETIFREVLDGREYLYWYSVQAEGRIEVEDSESYIDKKHLEYWEECIDPSYGMVGLDP
                     QVIMIHKTIYETMEELDRQYDETFKK"
     gene            complement(8394..8735)
                     /locus_tag="AC1PNG_001283"
     CDS             complement(8394..8735)
                     /locus_tag="AC1PNG_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIP6701789.1"
                     /translation="MREDIQINERALTVSEQLVEVLESIYDPEIELDIYNLGLIYEIH
                     LDEAGFCKVVMTFTDSGCSCADTMPGELVAALKTIDGINDAQVEIVWSPAWKMTRISR
                     LGRITLGISPK"
BASE COUNT         2501 a         2026 c         1740 g         2490 t
ORIGIN      
        1 aaaaaccagc tcttttattc attttcagag ataggggaaa aaagggaatg acatcccctc
       61 atccgccaac ccaaacctta aatgtattgc catcactcaa agtaaagctg actatccccc
      121 tatctaccat gattgtgttt aattatagat tttgaatgcg tcgtcatcat tgcgtccgac
      181 aaatggcatt gctttgcgga gttctgcacc tactttttcg atttcaagat tctctgcttc
      241 tttacgataa gcttccatac gtggacgacc tgctttatag tcatttacaa agtcatttgc
      301 aaatttacca gattgaatat ctgcaaggac tgccttcatg ttttctttga cttggtctgt
      361 aatcacgcgt ggacctgata catagtcacc aaattcagca gtgtttgaaa tagattggcg
      421 cattttctta aagccacctt cgtagacaag gtcaacgatg agtttcatct cgtggagaac
      481 ttcgaaataa gccaattctg gtgcataacc agcttctgtc aagacttcaa aacctgcctg
      541 cataagggct gtcaaaccac cgcagagaac agcttgctca ccaaacaaat cttcttctgt
      601 ctcttctttg aaagttgttt ccaaaagacc aacacgggct gaaccaacac cttttgccca
      661 gtccatcgca atatgttttg cattgccagt tgcatcttgg taaacagcat agagggctgg
      721 tactccaaaa ccttcttcaa aggtacggcg gaccaagtga cctggacctt ttggtgcaca
      781 catgaagaca tccacatctg ctggtacctt gataaattca aagtgaacgt tgaaaccatg
      841 ggcaaaacca agagcattgc ccgcttccaa gtttggagcg atttcttcat tgtaaaggtc
      901 tgcttggatt tcgtctggag ccaagatcat gataacatct gcttgttttg ctgcttctgc
      961 tacttcaaaa gtttcaaaac cgtcttcttt tgccttatca aatgacttac ctgcacgcac
     1021 accgatgatg acatcgtgcc ctgtatcacg caagttttga gcatgggcat gaccttgtga
     1081 accataaccg ataacggcga tacgtttacc gtcaagtgct gctactgtta catctttttc
     1141 atattgcatt gttactgtca taattttttc tcttttctat ttttatttgt agttttggat
     1201 gttcaaagaa aatgaaggct agactaggcg aagatagaac taacgttcat caagcgaagt
     1261 caacgcagga taggttttat tatcgaaaag gattagtctc tactaaagcc agtcgcaccc
     1321 gttcttgcga tattcttgat gccataaggc tgaatcacac gcaggagggc gtcaatctta
     1381 tctccatcac ctgtcatttg aatggtaatg gagtgcggtg ccacatcaac aacatttgct
     1441 ctaaagggtt ggataatcgc taaaatttct gctcgttttg ttggcggagc tgaaatttta
     1501 acaagaatca cctcacgctc taagtgggga atgtccgtca aatcacggac acggaccaca
     1561 tcaatcaagc ggttcagctg tttgatgatt tgttcgacct catcgtgact gaccacgtct
     1621 acaatcaccg tcacacgtga gatgccatct acttctgttg ggccgacgga gatggactca
     1681 atattgattt ggcgacgaga taggacacct gtaaaacgat tcagaacacc agatgaattt
     1741 cgcaatttag ctgttaacat tctacgcatt gaacttcacc cccaacattt ctgcattgct
     1801 cttacctgct ggcaccatcg gttgaacatg ttcacgatta gaaatatgaa cctctatgag
     1861 cattggttta tcttctaaga taactttcat gtcttcactt agagtcgctg gattgtcaaa
     1921 actataatga ctaattccgt aggcctctgc caacaattgg aaattcggtt catcgtcaaa
     1981 tacagattgg ctacgacgct tttcatagaa ggattcctgc cattgacgaa ccattccaag
     2041 agagtggtta ttgattaata caaccttaat cggtacacca tagccattca aaatagccaa
     2101 ttcttggttg gtcatttgga agccaccatc accgacaaag atgattactt ctttatctgg
     2161 attggctaat ttggcgccga tagctgcagg aataccaaag cccatggtcc ccatgccacc
     2221 agatgttaca agttgacgtg catgtttgta ggggtagaat tgagcggccc acatttgatg
     2281 ctgaccaacg tctgtcacga caatggcatc cccttgcgtc aactgaccaa ttaattcaat
     2341 tgccacctgt ggcttaataa ccttttcatc ttcatcatac caaaatggtg cccgacgttt
     2401 gttttccaat acttgtgccg tccaatcagc atagcctgtt tcgactgttt ctaaagcaag
     2461 tagagctttc aaggttgcct tagcatcgcc tactactgga atagccgttt tgacaacttt
     2521 accaatttct gcgggatcaa tatcaatatg tgctaccgtc gcatttggcg cataggttgt
     2581 tggatttcct gttaggcggt catcgaatcg cgctccaata ttgataatgt aatcagcctg
     2641 atccatagcc atattggcag cgtatgaacc gtgcatcccc cccatagcca aggataattc
     2701 atgctcaatg ggcatcgcac ctaatccaag gagggttgaa actactggaa tacggtagcg
     2761 ctctgcaaag gcaactaatt ctttattggc atctgcataa ttgacaccac caccagccaa
     2821 tataactggt ttttgagctt ggctaagctg tttcaaaatt ttcttaacct gcaaaccatt
     2881 tggttcaatc gttggctgat aacttggtag gtgtagagtt gggtcatgat aaaaatcaac
     2941 aactctttct tggatatctt ttggaacatc aataacaacg ggacccggtc tccctgttgt
     3001 tgcaatatgg agagcttctg tgatgactct aggaatatct gccgtatccc gaatttggta
     3061 attgtacttg gtaatcggca ttgtcatccc caagacatct gcttcctgaa aagcatcttt
     3121 cccgatacca cgtgttgcga cttgaccagt aaaaactaaa agaggaacac tatcacccat
     3181 tgcgtccgca attccagtaa tggcattggt agctcctggc ccagaggtta caatggctac
     3241 gcccaccttt cctgatgatt tagcgtatcc ttcggcctcg tggaccgctc cttgttcatg
     3301 acgagccaaa atatgctgga ttccctcata ttgatagata gcgtcatata gaggcaaaac
     3361 tgccccacca ggataaccaa aaacaaggtc tactcctagt tgatgcaggg tgtccaaaat
     3421 gagataggac cccgaacgcg gttggtctag ttgaatttct tgcacgaata ttcctcctct
     3481 ctgcaaattt gttatctact ataatatcac aaggaagtag aatgtcaagg aattttctga
     3541 aaattcctac atttgttcgt tttcttaact atttcctaac atttcttaag caaaacttaa
     3601 gcaaatccct tgactggctt ggtttcagct tgctttcaga aaacatcagt atagtagttc
     3661 cactaaaaaa attaaatgag gtttaggata tgtattatgt agtgataccg gcttaccagc
     3721 cagatgaaaa attaatccaa ctattagaaa taatgaaaaa caggctaaat tgtcaggtta
     3781 tcgttgtgga tgatggtagt actggcgcat ccaaaaatat tcttgaaatc gccaaaacct
     3841 atgcaattgt tctccaccat gatgtcaaca aaggaaaagg ccaagccttg cgaacagcct
     3901 ttagtttcat ccaagatttg agaaaaccag gtgttatcgt aactgctgac gcagatggtc
     3961 aacacactgt aaatgatatt gaccgtgtcg cacgcgccgc catgaatcta ccaagccgcc
     4021 tgattcttgg tgttcgtcaa tttactaaag acatcccgct ccgtagccga tttggtaaca
     4081 aactaaccag agtattgttt agattgcaaa ctggtgtcaa tgtgtccgat acccaaacag
     4141 gcttgcgtgg attccatact gacttaattc cattcatgtt agatattgaa ggcgaccgct
     4201 atgagtacga gatgaacatg ttaactcagg caagtcaacg ctataaaatt acggaagttc
     4261 caattcaaac tatttatatc gatgataatg ccagttcgca ttttcgtccc ataaaagatg
     4321 gactgctcat ttacaaaaat ctcttcaaat ttgccctggc atcttttggt ggatttctag
     4381 ttgactacgg aatatatgca cttgccttat tgctactcac tagcttacca actgctctac
     4441 gcttattggt cgcaaacacg attgctcgca tttgtagcgc tacctgtaat tttgcactta
     4501 ataagaaatt agtctttaac aatgaagaga gcatcgcacg aacaggcgca ggctacttta
     4561 cactagccct tgtcctcttc ctttgcgata cagccctcct ctatctcttc caccaaattc
     4621 taggacttaa cctctacatt gtcaaacttg cagttgatat cttccttttc ctagcctctt
     4681 ggtttgttca aaagaatatt atttttaaag aaaggaaatc attttcccat gaaattgcta
     4741 aaaaaaacct ttgcctacgc agccatcttc ggaatgcttt tgacaggagg ctttacttac
     4801 tccatgttaa aaaccttcgt gctttccgag gctatcacaa cggttgctgc aagtaatgcc
     4861 acagccaata gccagaacac agctacttca tcgtcatcaa ctgccgcaac cactgcaact
     4921 aatgtctcta caacggatac tagttactct gatgataata ttcagataaa tttagagacg
     4981 attaccacca ataacacaac tgtttacgtc gcagatattc aggtcagctc tccagagtat
     5041 ttaaagacag ctctagccca aaatacctat ggcacgaacg taacagctaa aacttctgaa
     5101 actgcgacag caaacaatgc cattctagcg gttaatggtg actactacgg agccaattca
     5161 actggttatg ttattaagaa tggcgttctc tatcgcgaca ctgttcgtga caatgctgct
     5221 tacggcgact tggcaatcta cgccgacggc tcatttgaag tcatctatga aaatgaaatc
     5281 actgcccagg aattgattga caagggtgtc gtcaacctct tagcattcgg tccatcattg
     5341 gtagagaatg gcgaaattgt cgtcgatact tcaacagaag tcggacgtgc aatgtcatcc
     5401 aaccctcgtt ctgctatcgg gattatcgat gaaaatcatt atatcatcgt cgtagcagat
     5461 ggacgtacgt ctgaaagcca aggactctct ctatcaattg gcagaagtta tgaaacaata
     5521 tggtgctcaa actgcctaca accttgatgg cggtggttca tccactcttt actttaacgg
     5581 tcaagttatt aacaacccaa taacaaatgg aaatactatc tcagaaaggg cggtgagtga
     5641 cattgtctac atcggttact aattcaacta caagagtttc aagaacattt atctcctact
     5701 tagcaataac agctttcctg tttattttca gtcgaatcta tgaaagtctc agctatggcg
     5761 aagtctctgt ctttatgcat tatatgttct gtataccatt aataggcgga gctgtgctac
     5821 tcggtctgct gcaacttata cctacccttt cacgtctctc ttataatttg tggaattctg
     5881 gcgttgcaac tattacagct ggttgtcttc tccaaggcat tatcaaccta tctggacgtt
     5941 caactacctt agatcaacct tattggtaca tcggcgcagc cttcctagtt ctatctttag
     6001 tcagtctttt tatcaatcca tccgcaaaac aatcgtcgaa aaacgaccac ttaatctaca
     6061 aataaattgt aatggcactt aacattctta ggtgcctttt actgtagaat gaattgttga
     6121 aaaattcgga aatttccaat tatttatcag atcttttatc gtttctttca tatctatgat
     6181 ataatgggta aaataagata gggagaagaa aattatgacc gatacaaaca aacttaaaga
     6241 ctttcgacat cgcagctctg tttacgattc gatggtcaaa tcgcccaacc gtgctatgtt
     6301 gcgtgccaca ggcatgaccg atgatagctt tgaaaagcct attgttgggg ttatttcgac
     6361 atgggctgaa aacacaccct gtaacatcca ccttcatgac tttggaaaat tagccaaaga
     6421 aggggtaaaa gatgcaggtg cctggcctgt ccaatacggt accatcaccg ttgcagacgg
     6481 gattgccatg ggaactcctg gtatgcgctt ctccttgact tcacgtgata tcattgcaga
     6541 ttctattgag gcagctatgg gaggacacaa cgtggatgcc tttgtcgcaa tcggtggctg
     6601 tgataaaaac atgcctggtt ccatgattgc catcgccaac atggatatcc ctgctgtttt
     6661 tgcctacggc ggaaccattg cgccaggtaa tctcaatggt aaagacatcg acttggtatc
     6721 cgtttttgaa ggaatcggaa aatggaacaa cggtgacttg accgctgaag aggttcgtca
     6781 aatcgagtgt aatgcctgtc ctggacctgg tggttgcggt ggtatgtaca ctgccaatac
     6841 catggctact gccattgagg taatgggcat gtctattcct ggctcttctt ctcaccctgc
     6901 tgaatcacca gagaaaaaag cagatattga agaagcaggt cgggccgttg tccgcatgtt
     6961 agaactaggt atcaaaccat ctgacattat gacgcgtgaa gcctttgaag atgccattac
     7021 agtcaccatg gcactcggtg gctccaccaa cgctactctg cacttgctgg ccattgccca
     7081 tgctgctaat gttgaattaa cactggaaga tttcaatgat ttccaagaac gcgtgcctca
     7141 cttggcagac ctgaaaccat caggaaaata cgttttccaa gacctctaca atgtcggcgg
     7201 cgtcccagct gtcatgaaat acttgctcaa aaacggcttc cttcatggcg accgcatcac
     7261 atgtacaggt aaaaccgttg ctgaaaacct agagaatttt gcagacttga ctccaggtca
     7321 agatgtcatc atgccacttg aaaatccaaa acgcgcagac ggtccactca tcatcctaaa
     7381 aggtaacctt gctcctgaag gtgccgttgc caaggtttct ggtgtaaaag tccgcaacca
     7441 tacaggtcca gccaaggtct ttgactcaga agaagaggcg atcgaagcag tcttgactga
     7501 cgaaatcgta gatggcgatg tagtcgttgt ccgctatgtt ggaccaaaag gcggtcctgg
     7561 tatgcctgaa atgctatccc tttcttctat gattgtcgga aaaggccaag gtgacaaggt
     7621 agcccttcta acagacggtc gcttctctgg tggtacctat ggactggttg ttggacacat
     7681 cgcacctgaa gctcaggatg gtggtccaat tgcctacctc cgtacaggtg atttggtgac
     7741 ggttgatcaa gataccaaag aaatcaccat gcacgtttcc gaccaagaaa tcgaagaccg
     7801 caagaaaacg acggtcattc caccactcta ctctcgtggt gtcctcggaa aatacgccca
     7861 cactgtatcc tcggcctcta aaggagctgt tacagacttc tggagaccag aacgtacggg
     7921 gaaaaaataa ggaactcaac gaccctgtcc tcgcggcagg gttttccaaa aggagaaaaa
     7981 atgaaactaa acgtcgaact ctcccgcttt cgtgttaaag aaggcaaaac cgttcaagtg
     8041 gatgaatgga tggcctttct caacgagcac atggaagaca cccttctcac tcttgaagga
     8101 gagaaaatgt atgtcgaaac catcttccgt gaggtattgg acggacgcga atacctttac
     8161 tggtactctg tccaagccga aggaagaatt gaagtcgaag attcagaatc ctatatcgac
     8221 aaaaaacatt tagagtattg ggaagaatgc atcgacccga gctatggtat ggtcggtttg
     8281 gatccgcaag ttatcatgat tcataaaact atctatgaaa ccatggaaga attagacaga
     8341 caatacgatg aaacatttaa aaaatgagct gctccatgca actcattttc tttttattta
     8401 ggactgatac caagcgtaat tcgacctaag cgactaattc ttgtcatttt ccaagcagga
     8461 gaccagacaa tctctacctg tgcatcgttt ataccatcaa tcgttttcag ggctgcaacc
     8521 aattcaccag gcatagtatc cgcacaagaa caacctgaat ctgtaaaggt cataacaacc
     8581 ttacaaaaac cagcttcatc taaatgaatc tcataaatca aacccaaatt gtagatgtct
     8641 agttcaattt caggatcgta aatggattcc aaaacctcta ccaactgttc tgatacagtt
     8701 aacgcacgtt cattaatctg aatatcttct ctcatcaagt tcaaacactc ctaaaat
//
LOCUS       DBNFEG010000084         8734 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.084, whole genome shotgun sequence.
ACCESSION   DBNFEG010000084 DBNFEG010000000
VERSION     DBNFEG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8734
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.084"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            24..5102
                     /locus_tag="AC1PNG_001284"
     CDS             24..5102
                     /locus_tag="AC1PNG_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIP6701790.1"
                     /translation="MKQKWSQIENKQRFSIKKLSVGVASVSIGFFITGVPMVQADTSG
                     EGLESTVAVATDMDSRQNSAVEKKEDGPLSDEPVKTEQVDEPVAEEGVVEEVVDTEAG
                     EESGLLTDQAATEIKTTAGKTTDESKEKEDISGKEASAPQTIPQESQLEPEEVTRGRY
                     ILQFSEENRNLVLDKLKKIDGVKIVHEYKEVLTGASVEVGKESLSDVKAITELTSLEE
                     SRRIRPTLHTAKQLVGALKASSKYQTDGRGMVIAVIDSGLDIKHKDMRLDDGVIPKIK
                     DITPSTTGTYTLKVPHGYNYVSGNDNLYDDTHEPHGMHIAGTLAGNATDEEVASKKGV
                     DGIAPNAQLLVYKIFSNDPKNYKAETEDAAYAAIEDAIKHGADVISLSVGYYDSGLPG
                     NAYYTIAKRAAEKGIIITAAIGNAGASSSDTSFDLHTNNALGAVDTATTVGVAATPAV
                     IAVGSARNTHLVQREFMLNGQSFGYYPIGYTTLTEGKYEFVDAGNGHWEEVQGLDLAG
                     KVAVIKKDKFDLKDAVRNLKFKDVAGIIVINTDQGWNKDYYRTHQLLVDDKTLLSYSS
                     IWGISLSGEDGRRLLEVANQSQGNTGLVLKPTIGMKKLIEVPTVSGFSSWGPTVNLEL
                     KPEIVAPGEDVYATLNDNRYGSMSGTSMASPIVAGASALLLPRIRQMTPPEGMTRMDL
                     LRIILMNTATPLVDVLDSSGHALENSPRQQGAGLLQIDRAFETDVILHHRLKGGVELK
                     EIGRETEFEVTLENLGNQQRSFAISAGKVLTSQDVPVDRIGRSGKVVKEIHATEIKGS
                     SIHLSEQSIQLGPKEKRTIRLKLDAGEAKDQFAEGYIYFKSLTDGQSDISIPYFGFVG
                     DWSKERIVDAPAWETSSKLKLTSVLSSYKHNKSGRYIELGREKIQDNQSPLNPDNIAI
                     QNQHSDSQIGNAFVRFALLRDITNYDLDIVKEATEDAPVLRRIDTGTMLSRVRYVDYF
                     ESLSEYFKLRTPIELHRWDGKVYDASKDENIPAPEGQYFFRLRVKNKENGAYQYTYLP
                     VKIDNQKPEIVAIDTNRLTSHRELVVTAKDNNKVWEVRANLNGEDLLVEKVVDDAGQL
                     HYHLKEVELPLDAKNHLRVEVMDIAGNVVAVEKDLVAPVIQFKNLEDLMAIRSKKTVE
                     IKANVSAQVSNVQANLDAQAVNYSLENGQLSLQIPEQSDGRHSFELILKDKDGKLIYT
                     KTLNYLVDNEKPTIDLDIEKDEEDEEVIQIGKNGRFTLKGKVSDNVSLPKNIKLYYSN
                     LDIGKGELKIIDVKEDGSFEQDFFKSDFPRAIMLTAVDEKGNKLKDLRINTSPESLDE
                     EEETEVPITVNNWLIDPIRFNKESLGRELDSGLVDFKKQEDGTYLFTFEIEAETDQAH
                     SVRINGGEKRYFEDGKLTYPVTLIEEGNVVDISVYNEADELTYTKKYQMLVDTENPVL
                     QLENEVLPLERQVVDSEEDEDEENQYAGVLLADADGHLTLTGSAKDNGIYWSLKINED
                     FVARGGFWRQYGNNEKAFRYELHSLKDGDTVKLDLSDSFGNALVKKYKVRLNDKEVSE
                     QVPEKDLHVEQSDKDQAPSIPVPKSEAHVPMPKEENSLAPQTESTEIALLTGDTREDG
                     VEHLVRFTKHEEALGISDERIEVSVPHREFFERSGRGETGALAADTSGKLPQTGDSLG
                     SVFISALLGLFGGAMVLGNLKRKE"
     gene            complement(5145..5215)
                     /locus_tag="AC1PNG_001285"
     tRNA            complement(5145..5215)
                     /locus_tag="AC1PNG_001285"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5181..5183),aa:Cys,seq:gca)
     gene            5756..6268
                     /locus_tag="AC1PNG_001286"
     CDS             5756..6268
                     /locus_tag="AC1PNG_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIP6701791.1"
                     /translation="MIVLITGASHTGKTVLAQKLLEKYHYPYLSIDHLKMGLIRSGNT
                     KLSVEEDDKLTAYLWPIVREMIKTAIENEQNLIVEGIYIPFDWATDFAIAYLDHIRYY
                     CLVMSENYIQKHFGSIKKHARLIENRIDDEWCTMESVLEENTRFFRRAKKYDANILWI
                     DESYEIEIDL"
     gene            6615..7391
                     /gene="rpsB"
                     /locus_tag="AC1PNG_001287"
     CDS             6615..7391
                     /gene="rpsB"
                     /locus_tag="AC1PNG_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIP6701792.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFIRDAAANDAVILFVGTKKQAAEAVKDEAIRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEINRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIADMPRIPDV
                     MFVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKMA
                     DAIIEGNQGEDSVAAVEAELAAEPASTESIEELVEVVEGK"
     gene            7644..8684
                     /gene="tsf"
                     /locus_tag="AC1PNG_001288"
     CDS             7644..8684
                     /gene="tsf"
                     /locus_tag="AC1PNG_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIP6701793.1"
                     /translation="MAEITAALVKELREKSGAGVMDAKKALVETEGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVDGNVAAVVEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPADNEAALKLAMPSGETLEEAYVNATATIGEKISFRRFALVEKTDAQAFGAYQHNG
                     GRIGVISVVEGGDETLAKQISMHIAAMKPTVLSYTELDEQFVKDELAQINHKIEQDNE
                     SRAMVDKPALPLLKYGSKAQLTDEVIAAAEEAIKAELAAEGKPEKIWDKIIPGKMDRF
                     LLDNTQVDQAYTLLAQVYIMDDSKTVEAYLNSVNASVVEFARFEVGEGIEKASNDFEA
                     EVAATMAAALGK"
BASE COUNT         2905 a         1466 c         2074 g         2289 t
ORIGIN      
        1 taatcttaaa aaacggaggt tttatgaaac agaagtggtc tcagattgag aataaacaac
       61 gctttagtat caagaagtta agtgtcggtg tggcatcggt atcaatagga ttttttatca
      121 cgggtgttcc gatggttcaa gcagatacta gtggagaagg attggagtct acagttgcgg
      181 tcgcaacgga tatggatagt aggcaaaatt ctgcggtaga gaaaaaagaa gatggaccat
      241 tatcagatga gccagttaaa acggagcagg tggatgaacc agttgctgaa gagggagtgg
      301 tcgaagaagt tgtagataca gaggcgggtg aagaatcagg tcttctcaca gatcaagctg
      361 cgaccgagat aaaaactaca gctggtaaga caacagatga gtcaaaggaa aaagaagaca
      421 ttagcggaaa agaagctagt gctcctcaaa caatcccgca ggaatcacag cttgagccag
      481 aagaggtgac aagagggcgc tatattttac aattttccga agagaaccga aatcttgtat
      541 tggataaatt aaagaaaatt gatggcgtta aaattgttca tgagtataag gaggttttaa
      601 cgggagcatc agtagaggta gggaaagaaa gtttgtctga tgtgaaggca attaccgaat
      661 taacctcttt agaagagagt cgccgtatcc gaccgactct tcatactgct aaacagctgg
      721 ttggtgcctt aaaagcaagt tctaaatatc agacagatgg tcgcggaatg gtgattgcag
      781 tcattgactc cggtttggat attaagcaca aagacatgag gttggatgat ggtgtcattc
      841 ctaaaatcaa ggacattact ccatctacga caggaacata cacactgaaa gttccccatg
      901 gatataatta tgtatctggt aacgataatc tctacgatga tacccacgaa ccacatggta
      961 tgcacatagc aggaactctg gctggaaatg caacagacga agaagttgcg tctaaaaagg
     1021 gagttgatgg gattgctccc aacgcacaac tgctagtcta caagattttt tcaaatgacc
     1081 ctaaaaatta taaagctgag acggaggatg ctgcttatgc tgctattgaa gatgctatca
     1141 aacacggggc agatgtcatc agcttgagtg ttggttatta tgatagcggg ttaccaggga
     1201 atgcttacta cacgattgct aagagggcag cagaaaaggg aattatcatt acagcggcta
     1261 ttggtaatgc tggtgcctcc tcttcagaca cctcctttga cctgcataca aataatgcct
     1321 taggagctgt ggatacggca acaacagttg gtgtggcagc gactcccgca gttatcgcgg
     1381 ttggttcggc gagaaatacc catcttgttc aacgagaatt tatgctgaat ggacagtctt
     1441 ttggttacta tcctattggc tatacaacgc ttacagaggg aaaatatgaa tttgtagatg
     1501 ctggaaatgg tcattgggaa gaagtgcaag ggcttgattt ggctggtaaa gtagcggtta
     1561 tcaaaaaaga taagtttgac ttgaaagatg ctgttcgtaa tttgaaattc aaggatgttg
     1621 ctggaattat tgtgataaat actgatcagg gatggaacaa ggattattat aggacccatc
     1681 agttgttggt agatgataag actttgcttt cctactcctc tatctgggga attagtctta
     1741 gcggggaaga tggaaggcga ttattagaag ttgccaacca atcgcaaggt aacactggtt
     1801 tggttcttaa acctacaatt ggaatgaaga agttgattga agtgccgact gtatcaggct
     1861 tttctagttg gggacctacg gtcaacttag aattgaagcc agagattgtg gcgcctggag
     1921 aggatgttta tgcaaccttg aatgacaatc gttatggctc catgtcaggg acttcgatgg
     1981 cttcgccaat tgtggcgggt gccagcgcac ttttattgcc acggattcgc caaatgacac
     2041 caccagaagg catgactagg atggatttgc tgagaatcat tttgatgaat actgctaccc
     2101 ctttggttga tgttctagat tcatctgggc atgctttgga aaattctccg agacaacaag
     2161 gtgctggtct gttgcagatt gatagagcct ttgaaacaga tgtgattctt caccaccgtc
     2221 taaaaggagg ggtggaatta aaagaaatag ggcgtgagac agaatttgag gtaaccttgg
     2281 aaaatctggg aaaccaacaa agaagctttg ctatttcagc tgggaaagtg ttgactagtc
     2341 aagatgttcc tgttgataga ataggacgtt ctggaaaagt agttaaagag attcatgcga
     2401 cagaaatcaa ggggtcgagt attcatcttt ctgagcaatc cattcaacta ggtccaaaag
     2461 agaagagaac cattcgcctg aaattggatg caggagaagc gaaagaccag tttgcagaag
     2521 ggtatattta cttcaaatca ttgacggatg ggcagtctga catcagcata ccttattttg
     2581 gctttgttgg tgactggtca aaagaaagga ttgttgatgc accggcttgg gaaaccagtt
     2641 ctaaattgaa actgacctca gttttatcta gctataaaca caataagtct gggcgctata
     2701 ttgaacttgg gcgtgaaaag attcaagata atcaatcgcc tctaaatcca gacaatatcg
     2761 caattcaaaa ccagcattcg gatagtcaga ttgggaatgc ctttgttcga tttgcactat
     2821 tgagggatat taccaactat gatttggata ttgtaaaaga agctacagaa gacgctcctg
     2881 ttttaaggcg aattgataca ggaactatgc tgtctcgtgt tcgctatgta gattattttg
     2941 aaagtctttc ggagtatttc aagcttcgta ctccgataga attgcaccgt tgggatggga
     3001 aagtgtacga tgcaagcaag gatgaaaaca taccagcacc ggaaggacaa tatttcttta
     3061 gattgcgggt aaaaaataaa gaaaatgggg cctatcagta tacctatctg ccagtcaaaa
     3121 ttgataatca aaagccagaa attgtcgcga ttgataccaa tcgtctgacc agccatagag
     3181 aactagtcgt cacagccaag gataataata aagtttggga ggttcgagct aatctaaatg
     3241 gagaagatct tcttgttgaa aaggttgtag atgatgcagg tcaactacat taccatctca
     3301 aagaagtgga actgccactc gatgctaaaa atcatctccg tgttgaggtg atggatatag
     3361 cgggtaatgt ggttgctgtt gaaaaagact tggtggcgcc tgtaatccag tttaaaaatt
     3421 tggaagattt gatggcaatt cgcagtaaga aaactgtaga aattaaagcg aacgtctcag
     3481 cacaagtttc caatgtacag gctaacttgg atgctcaggc tgtgaattac tctctggaga
     3541 atggtcaact atctctccag attccagagc aatcagatgg acgtcatagt tttgaattaa
     3601 tcttgaaaga taaggatggc aagcttattt acactaaaac cttgaattat ttggtagata
     3661 atgaaaagcc gacgattgat cttgatattg aaaaagatga agaggatgaa gaggttatcc
     3721 agattggcaa aaacgggcgt tttacactga aggggaaagt gagtgataat gttagtcttc
     3781 ctaagaacat caaactttac tattccaatc tagacattgg taagggagag ctgaagatca
     3841 tagatgtaaa agaagatggt agttttgagc aagacttctt taaatctgat tttcctagag
     3901 ctatcatgtt aactgcggta gatgagaagg gaaataaatt aaaggacctt cgtatcaata
     3961 caagtccaga gagtctggat gaggaggagg aaacagaagt tccgattact gttaataact
     4021 ggttgattga cccgattcgt ttcaataaag aaagtcttgg tcgtgagcta gatagcgggt
     4081 tggtcgactt caaaaagcaa gaagatggga cttatctatt tacatttgaa atagaagcag
     4141 aaacggatca agctcattct gtgcgcatca atggtggtga aaaacgctat tttgaggatg
     4201 gcaagttgac ctatccagtt accctgattg aagagggcaa tgttgtggat ataagtgtgt
     4261 acaatgaggc ggatgagttg acttacacga agaaatatca gatgttggtt gatacagaaa
     4321 accctgtttt acagctggaa aatgaggtgc taccattgga aagacaggta gtggatagtg
     4381 aagaagatga agacgaggaa aatcagtatg caggagttct cctggcagat gctgatgggc
     4441 acttgacact taccggaagc gccaaggata atggaattta ttggtccttg aagatcaacg
     4501 aagattttgt agctcgagga ggtttctgga ggcagtatgg aaataatgaa aaagcctttc
     4561 gctatgagct acatagtctg aaagacgggg atacagtcaa acttgatttg agtgacagtt
     4621 ttggaaatgc tcttgtgaag aaatataagg tacggttaaa tgacaaggaa gtatctgagc
     4681 aggttcctga aaaagacctt catgtggagc aatcagataa ggatcaagcc cctagcattc
     4741 ctgttccgaa atcggaagcg catgtaccaa tgccaaaaga agagaattcg cttgctccgc
     4801 aaacagaatc cactgaaatc gcgttgttaa ctggcgatac aagagaagat ggtgtggagc
     4861 atttggtgag atttacaaaa catgaggagg cactaggaat ttctgatgag agaatagaag
     4921 tgtccgtacc tcacagagaa ttctttgaaa gaagcggaag aggagagaca ggtgcactag
     4981 cggcggatac tagtgggaaa ctgcctcaaa ctggagatag ccttggaagt gtctttataa
     5041 gtgctttgtt aggtctattt ggtggagcta tggtcctcgg aaatctgaag cgaaaagaat
     5101 agcaaaaaac ctaggaagat tcctaggttt tttagaatat taaaaggcgg tagacggatt
     5161 tgaaccgacg atcaagcttt tgcagagccg tgccttacca cttggctata ccgccacaac
     5221 aaagaatatt ctaccgaaaa aaaagctgaa agtcaagaaa aatctagtga ctgtttgtga
     5281 ctgataacaa atctataaaa cagttgcgaa aacataggag aatacgacga aaaagatagg
     5341 agtacttttt gagtattgat ggctagtacc actctctttg tttgatagaa aaagagtgga
     5401 gcaaaaagaa ttaagaaaca ctttgggtaa gtgaattcat ttttactgca aggaatgtta
     5461 agggaacaga attacgataa tagtgcacct ctttaagaaa gatttggagg tgatagagtt
     5521 tatttttgct aaataaagat taaaccaaga agaatattcc atctaaaacg gttgttttca
     5581 tttacagtat attgcaaaga taactcaggc acttgattag attaactgaa aaaatgtggg
     5641 aacatgatat aataaattga gtaagagatt gaaaaataga taggatgaca agcagtgttt
     5701 cttacaaatt ttcatataaa acatagatgc tttgtcgctt agataggaga aaatcatgat
     5761 tgttttaata acgggagcat ctcacactgg gaagactgtg ctggctcaaa aattacttga
     5821 aaaatatcac tatccttatt tatcaataga ccatttgaaa atggggttaa ttcgtagcgg
     5881 caatactaaa ctttcagtcg aagaagatga taaacttaca gcatatctat ggcctattgt
     5941 tcgtgaaatg attaaaactg ccatagaaaa tgagcaaaac cttattgttg aagggatcta
     6001 tatccctttt gattgggcaa ccgactttgc aatagcctac cttgaccata tcagatatta
     6061 ctgtcttgtg atgagtgaaa actatattca gaaacacttt ggaagcataa agaaacacgc
     6121 acgccttata gaaaatcgta tagatgatga atggtgtacg atggaaagtg ttttagaaga
     6181 aaatactcga ttttttagaa gggcgaaaaa atatgatgca aacatcctat ggattgatga
     6241 gagttacgag atagagattg atttatgata gagagacatt gtatcacagt ttatctaagg
     6301 gatacaacta agaggaagta acatcagata ggcgttactt cctttttatt tctaagaaat
     6361 ataaagtaaa atggatgaag ttaaaaatca taaaaaagta gtaacataaa gagagacata
     6421 tgccaatcta tgagcttggc ataatataga gatttgtgta ctgaaatgct tgattttcac
     6481 ctaaaacttt ggtatactta ctaagttgtc taaacaacaa atactcatct cggaccaatt
     6541 gtggtcttgt tgccgaaagg ttgggctgca agtcgaagtt cgggagagga gaaaaaacaa
     6601 aaggagaaat actcatggca gtaatttcaa tgaaacaact tcttgaggct ggtgtacact
     6661 tcggtcacca aactcgtcgc tggaatccta agatggctaa atacatcttc acagagcgta
     6721 acggtatcca cgttatcgac ttgcaacaaa ctgtaaaatt ggctgaccaa gcttacgaat
     6781 tcatccgtga cgctgcagca aacgacgcag ttatcttgtt cgtaggtact aaaaaacagg
     6841 ctgctgaagc tgttaaagac gaagctatcc gcgctggtca atacttcatc aaccaccgtt
     6901 ggttgggtgg aactcttaca aactggggta caatccaaaa acgtatcgct cgtttgaaag
     6961 aaatcaaccg tatggaagaa gatggaactt tcgaagtgct tcctaagaaa gaagtagcat
     7021 tgttgaacaa acaacgtgct cgtcttgaaa aattcttggg cggtatcgct gacatgccac
     7081 gcattccaga cgtaatgttc gtagttgacc cacataaaga gcaaatcgct gttaaagaag
     7141 ctaaaaaatt gggtatccca gttgtagcga tggttgacac aaacactgat ccagatgata
     7201 ttgatgttat catcccagct aacgatgacg ctatccgcgc tgttaaattg atcacagcta
     7261 aaatggctga cgctatcatc gaaggcaacc aaggtgaaga cagcgtagca gcagttgaag
     7321 ctgaattggc agctgagcca gcatctacag aatcaatcga agaattggtt gaagttgtag
     7381 aaggaaaata agtaacgaag gcgtttggaa atctgtatga aaataggaaa ctgacgcagt
     7441 atgcttgcat acaaggaagt ttatcttttt cactaagatt ttagcctgag tacaattgaa
     7501 taagtaatga aggcgaatta aaatcaatac ggttttagct cgcgtataat tctacatcat
     7561 tatttaacaa caaaaaaaca atcctagagg ggcagggctg agcccgctcc tctattttat
     7621 aaatacaaac aaggagaata gacatggcag aaattacagc agctctcgtt aaagaattgc
     7681 gtgaaaaatc aggtgctggc gttatggacg caaaaaaagc attggttgaa actgaaggtg
     7741 atatcgaaaa agcgattgaa ttgcttcgtg aaaaaggtat ggccaaggca gctaagaaag
     7801 ctgaccgtgt agcagctgaa ggtttgacag gtgtttacgt tgatggtaac gtagcagcag
     7861 ttgttgaagt gaacgctgaa acagacttcg ttgcgaaaaa cgctcaattc gttgagttgg
     7921 taaacactac tgctaaagta attgcagaag gcaaaccagc tgacaacgaa gcagctctta
     7981 aattggctat gccttcaggt gaaacccttg aagaagcata cgtaaacgca actgcaacta
     8041 tcggtgagaa aatctcattc cgtcgttttg ctttggttga aaaaacagat gctcaagcat
     8101 ttggtgcata ccaacacaac ggcggccgta tcggtgttat ctcagttgtt gaaggtggcg
     8161 acgaaactct tgcaaaacaa atctcaatgc acatcgctgc gatgaaacca actgttcttt
     8221 cttacactga attggatgag caattcgtta aagatgagtt ggcacaaatc aaccacaaaa
     8281 tcgaacaaga caacgaaagc cgtgcaatgg ttgacaaacc agcattgcca ttgttgaaat
     8341 acggttcaaa agctcaattg actgacgaag tgattgcagc agctgaagaa gctatcaaag
     8401 cagaattggc agcagaaggc aaaccagaaa aaatctggga caaaatcatc ccaggtaaaa
     8461 tggatcgctt cttgcttgac aacacccaag ttgaccaagc ttacacactt cttgcacaag
     8521 tttacatcat ggacgacagc aaaacagttg aagcttactt gaactcagta aatgcttcag
     8581 ttgtagaatt tgctcgtttc gaagtaggtg aaggtatcga gaaagcttca aacgactttg
     8641 aagcagaagt tgcagcgaca atggcagcag ctcttggtaa atagtagata gttaaaaaag
     8701 ctaccttaga aggtagcttt ttctgtgggc ttat
//
LOCUS       DBNFEG010000085         8554 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.085, whole genome shotgun sequence.
ACCESSION   DBNFEG010000085 DBNFEG010000000
VERSION     DBNFEG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8554
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.085"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(324..1154)
                     /locus_tag="AC1PNG_001289"
     CDS             complement(324..1154)
                     /locus_tag="AC1PNG_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIP6701794.1"
                     /translation="MKKMMLGALALVTSLTLAACGSNDSATKSDNWTAYESEKSVTIG
                     FDKTFVPMGFEQTDGSYTGFDIDLANAVFEKYGITVKWQPIDWDLKETELNNGNIDLI
                     WNGYSITDERKEKVLFTNPYMDNQQVLVTKKSSNISQVSDMKDKILGAQAGSSGYSVF
                     ESQPAILKDIVQNNDASQYATFNEALIDLKNDRIDGLLIDRVYANYYLQQEGIIADYN
                     IIDAGFENEAFAVGARKSDTTLVENINKAFAELYKEGKFQEISQKWFGEDVATDAVKK
                     "
     gene            complement(1181..1813)
                     /locus_tag="AC1PNG_001290"
     CDS             complement(1181..1813)
                     /locus_tag="AC1PNG_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIP6701795.1"
                     /translation="MLELRNLSKRFEDKQIFSNYDLVIPEGKIVAIVGQSGGGKTTLL
                     RMLAGLETIDSGTLMYNGQELPLEELGKRHLLGFVFQDFQLFPHLSVLENLVLSPMKT
                     QNMSRSEAEDKALKLLDTLGLANHATAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRKEVEKLILENRATGITQIVVTHDMQFAENIADEIIKIEPKH"
     gene            complement(1823..2464)
                     /locus_tag="AC1PNG_001291"
     CDS             complement(1823..2464)
                     /locus_tag="AC1PNG_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIP6701796.1"
                     /translation="MDYVLEVLPSLLNGAAVSLQVFFLVLILSLPLGAVFAFLMQIKF
                     KPLQWLLHFYVLIMRGTPLLLQLIFVYYVLPSVGIIFDRMPAVILAFTLNYAAYFSEI
                     FRGGIEAIPKGQYEAAKVLKFTPVQTIRYIVLPQVVKIVLPSVFNEVMTLVKDTSLVY
                     ALGVSDLLLASRTAANRDASLAPMFIAGAIYLLMIGAVTLISKQVEKKFDYYR"
     gene            complement(2628..4439)
                     /gene="glmS"
                     /locus_tag="AC1PNG_001292"
     CDS             complement(2628..4439)
                     /gene="glmS"
                     /locus_tag="AC1PNG_001292"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIP6701797.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVTGGEQAHL
                     VKAVGRIAELSAKVGDKTEGTTGIGHTRWATHGKPTENNAHPHTSQTAGHILVHNGVI
                     ENYAEIKEEYLAGHDLKGQTDTEIAVHLIGQFAEEGLSTLEAFKKALKIIQGSYAFAL
                     IDATDADTIYVAKNKSPLLIGLGDGYNMVCSDAMAMIRETSEYMEIHDKELVIVKKDS
                     VEVMDYDGNEIERGSYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLISAYTNEAGQ
                     VTVDADIIKAVQEADRIYILAAGTSYHAGFASKDFLEKLTDTPVELGISSEWGYNMPL
                     LSKKPLFVMISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREATYTMLLHAGP
                     EIAVASTKAYTAQIATLAILAKAVGDANGNAYAKEFDLVHELSIVAQSIEASLSEKDV
                     IAEKVEKLLSTTRNAFYIGRGSDYYVSMEASLKLKEISYIQCEGFAAGELKHGTISLI
                     EDGVPVLALISNHPHLASHTRGNIQEVVARGANVLTIVDEAVAKEDDDITVTTVHPFL
                     SAIAMVVPTQLIAYYATLQRGLDVDKPRNLAKSVTVE"
     gene            4746..6002
                     /locus_tag="AC1PNG_001293"
     CDS             4746..6002
                     /locus_tag="AC1PNG_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HIP6701798.1"
                     /translation="MEQLYHTTELIGIKDKNIKILFILKHQTHLEIRAKLDYDSPGCP
                     HCQGKCIKYDFQKSSKIPILDCQGFPTLLLLKKRRFQCKSCQKVTVAETALVKKNCQI
                     SLPIWEKVTQLHTENMTNIAIARRLHISVSVVQRKLAQFQFEHDFTKLPKVLSWDEFS
                     RNKGKLAFIAQNFETRSIVTILDNNRQTTIKNYFYKYPRAVRETVEVVTVDMSGSYIP
                     ITKKLFPRAKIVLDRFHIIQHLSRAMMTTRIDIMKTFDTRSLPYRSMKNHWRILQKDS
                     RKLSLNRFFSRTFGQTVTPREVVQKTLNFSEELKFYYELYQILLFHFQEMNSKYFFEL
                     LEDNLDLVNPAFKTVFKTFLKYKTYITNAMELPYSNAKLEATNKLIKDIKRQAFGFRN
                     FTNFKTKIYIALNIKNERTNFVLSRC"
     gene            complement(6052..6594)
                     /gene="lepB"
                     /locus_tag="AC1PNG_001294"
     CDS             complement(6052..6594)
                     /gene="lepB"
                     /locus_tag="AC1PNG_001294"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIP6701799.1"
                     /translation="MVKRDLIKQISLLVLLILGIIGLRFWLLEPVTITPEMANSYLKE
                     NDFIMTVRNVRPIHGDFILYNHEGKEYVSRVIALENETVTYMDDVLYRNDIIITENYL
                     KTPHSQESYTDDFTLETLTNGKYNVIPEGHYLVLNDVRTNQQDSRSFGLISSEAIVGR
                     LTFRISPLSEFGFIKTGLVQ"
     gene            complement(6637..7254)
                     /locus_tag="AC1PNG_001295"
     CDS             complement(6637..7254)
                     /locus_tag="AC1PNG_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIP6701800.1"
                     /translation="MSDIMYPEVLTVGSGAVKVATVGDSLTYGYGLENREKDAYPCIL
                     AEKLGHHYQVSNYGLSGRSLQSTADFPYFKEKNAQLSLESEADIVIIMIGSNDSRGPY
                     WNRERFIREYREMAEQYMDLPSQPDVYLVIPPYVPTSRFGLNNQIIEDELQKIIPAIG
                     NELGLPVINLYTVTEGHLEYYSDGLHLTPLGNQVIAEEIYQHLRH"
     gene            7339..8448
                     /locus_tag="AC1PNG_001296"
     CDS             7339..8448
                     /locus_tag="AC1PNG_001296"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIP6701801.1"
                     /translation="MKKQVIIIGGGIVGSTVAFYLSQEEQVNLTLIDSGIGTATRAAA
                     GIICPWMAQKKNKDWYKLTSDGAVFYRQLVADLEKSGATEIPFKQTGTIGLKSKPELL
                     DKIQKIAEERRVDTPTIGEITPLIGSEINQYLPLLKPDYFGIHLEGGGRIDGGRLIDI
                     LQEQFLKNGGTRIYGQAKLLDNHTVEVDNQVLTADHIILATGAWLPHILEPLGYQVDV
                     RPQKGQLFELDTEFDTDNWPVCMPYGEIDILPFENGKIIIGATHEDDMGYDLELDPEK
                     IQAMHDKIAEFMPDLANYPVARTRIGTRAYTSTYSPFYGNIEDMPNVWVASGLGSTGL
                     TNGPIIGWQIAKEILNQETNFDRTPYSPNRYILKK"
BASE COUNT         2659 a         1870 c         1508 g         2517 t
ORIGIN      
        1 tctatcccca gacatcaatt tatgtgagct gactgccgtc agcttatatc tcccacttta
       61 aaaggtctcc cagacctttt aaactcccac ccccgcatag ctccaacagt cagaatagtg
      121 actgttggag ccaatcaacc actgcgctga gatgttgaca cgaactccga gaagtggcgc
      181 tgagcttttt gctcagcctc gtagaatctt aagttaatga tattggtgta cacccccacc
      241 gttttcacga gttcttttca ttattctgtg gatgagagcg taaccatcat ggatgtcttg
      301 ttcagtcact ttttcttaca ttattatttt tttactgcat cggttgcaac atcctcacca
      361 aaccattttt gggagatttc ttggaattta ccttctttat ataattcagc aaacgcctta
      421 ttaatgtttt ctacgagtgt cgtatctgat ttacgagcac cgactgcgaa ggcttcattt
      481 tcaaaacctg catcaatgat gttgtaatct gcgataattc cctcttgttg taagtaataa
      541 tttgcataca cacggtcaat caaaaggcca tcaatacgat cgtttttcaa gtcaatcaag
      601 gcttcattga aggtcgcata ctgacttgca tcgttatttt gaacaatatc tttcaaaata
      661 gctggctgtg attcaaatac tgaataacca gatgaacctg cttgcgcacc taaaatctta
      721 tccttcatat cagatacttg gctaatattg gaagattttt tagttacaag aacttgttgg
      781 ttatccatgt agggatttgt gaaaagaact ttttccttac gctcatctgt aatagaataa
      841 ccattccaaa ttaaatcaat gttcccgtta ttcaattcag tttctttcaa gtcccagtca
      901 attggttgcc attttacagt aattccatat ttttcaaaga ctgcattagc caagtcaata
      961 tcgaaacctg tgtaagaacc atctgtctgt tcaaatccca ttgggacaaa ggtcttatca
     1021 aagccaatcg taacggattt ttcagactcg taggctgtcc aattatcaga cttagtagcc
     1081 gaatcattgg agccacaagc agcaagagtc aaagaagtta caagagcaag agctccaagc
     1141 atcatttttt taatattcat catcatattt gttccttttc ttagtgtttc ggttcaattt
     1201 tgatgatttc atctgcgata ttctcagcaa actgcatatc atgggtgacc acaatttgcg
     1261 tgattccagt agccctattt tcaagaatca gtttctcaac ttcttttctt aattcagggt
     1321 ctagggccga agttggttca tcatagccga taatctcagg atcaatcatc atcgcacgcg
     1381 ccaaggccac acgttgtttc tgtccacccg ataatgaaaa tgggtaggca gttgcgtggt
     1441 tagcaagtcc aagagtatcc aagagtttaa gggccttgtc ttccgcctct gaacgcgaca
     1501 tattttgcgt tttcatagga gagagaacca agttttccaa tacggatagg tgcgggaaaa
     1561 gctggaagtc ctggaagaca aagcccagca aatgtcgttt tcccaactcc tctaatggca
     1621 gttcttgacc attatacatc aaagttcctg aatcaatagt ctctaaacca gccaacatcc
     1681 gtaaaagagt tgtcttacca ccgccagatt gaccaacgat ggcaacaatt tttccttctg
     1741 gaatgaccaa atcatagttg gagaaaatct gtttatcttc aaagcgtttt gacaaattgc
     1801 gtaactctaa catggcttcc tcctatctat aataatcaaa tttcttttct acttgtttag
     1861 aaatcagtgt taccgcaccg atcatcaata ggtagattgc acctgcgata aacataggcg
     1921 ccaagctggc atcacgattg gccgcagtac gactagccaa caacaaatca ctaaccccaa
     1981 gtgcatagac caaagaagta tcctttacca aagtcatgac ttcattgaaa acacttggca
     2041 gaacaatttt cacaacctgt ggtaagacaa tgtagcgaat ggtctgaaca ggggtaaatt
     2101 tcaatacctt agcagcttcg tactgacctt ttggaatagc ttcaatacca ccacggaaaa
     2161 tttcagagaa ataagccgca tagttgagcg taaaggccag aatcacagca ggcattcgat
     2221 caaatataat acccacactt ggtaagacgt aataaacaaa aattaattgt aaaagcaagg
     2281 gggttcctcg cataattaat acatagaaat gcaataacca ttgtaagggt ttaaatttaa
     2341 tctgcattaa aaaagcgaaa acagctccta acggcaagga taatatcaat actagaaaaa
     2401 atacttgcaa ggagactgca gcaccattta gtagactggg cagaacttcc aaaacataat
     2461 ccatagagct ctcctaaaag aatgattagg ttctagtata tcaaaaaata tccttaattt
     2521 caaagaattt ttcagaattt ttaataaatt taacaaaatt taacaaaatt ttgtatattt
     2581 tttcaacagt gcaaaaagaa ggctggaaat ccacgccttc cttcttctta ttcaacagta
     2641 acagacttag ccaagttacg tggtttatca acatccaaac cgcgttgcaa tgtcgcatag
     2701 taagcgatca actgtgtcgg aaccaccata gcaatcgctg acaagaatgg atgaacagtt
     2761 gtaacagtga tgtcatcatc ttctttagct actgcttcat ctacgattgt taagacattt
     2821 gcaccacgcg caacaacttc ttgaatatta ccacgagtat ggctagcaag gtgcggatga
     2881 tttgaaatca aggccaagac tggtacacca tcttcaatca aggaaattgt gccgtgtttc
     2941 aattcaccgg ccgcaaagcc ttcacattgg atgtatgaga tctctttcaa tttcaaactt
     3001 gcttccatag aaacgtagta gtcactacca cgaccgatat agaaagcgtt gcgagtagtc
     3061 gataagagtt tttcaacttt ttcagcaatc acatcttttt ctgacaagct ggcctcgata
     3121 gattgggcaa caatggacaa ttcatgcacc aagtcaaatt ctttcgcgta ggcattgcca
     3181 ttggcatcac cgacagcctt agccaagatt gccaaagtcg caatctgtgc agtgtaggct
     3241 tttgtagaag ctaccgcaat ttctggtcct gcatggagca acatggtgta agttgcctca
     3301 cgagataggg tagaaccagg aacgttggtt acagtcaaac ttggaatgcc catttcatta
     3361 gctttaacca atacctgacg gctatcagca gtttcacctg actggctaat catgacaaag
     3421 agtggctttt tgctcaaaag cggcatattg tagccccact cagatgaaat tcccaactct
     3481 actggtgtat ctgtcaattt ttccaagaag tcttttgagg caaatcctgc gtgataggaa
     3541 gttccagcag ccaagatata gatgcggtca gcttcttgta ctgccttaat aatatccgcg
     3601 tcaactgtta cctgaccagc ttcatttgta taggcgctaa tcaatttacg cataacagtt
     3661 ggttgctcat caatttcctt gagcatgtag tatggataag ttcccttacc aatatctgac
     3721 agatcaagct cagctgtata gctaccacgt tcaatctcat ttccatcata gtccatgact
     3781 tcaacactat cttttttcac gataaccaat tccttatcgt gaatttccat gtattcactt
     3841 gtctcacgaa tcatagccat agcatctgag cagaccatgt tatagccatc accaagacca
     3901 atcaaaagtg gagatttatt tttggcaacg tagattgtat ctgcatccgt agcatcaatc
     3961 aaagcaaagg catatgaacc ttgaataatc ttcaatgctt ttttaaaggc ttctagagtt
     4021 gacaaacctt cttctgcaaa ttgaccaatc aagtgtacag caatttctgt atctgtctgc
     4081 cctttcaagt catgacctgc aagatactct tccttgattt ctgcgtagtt ttcaatcaca
     4141 ccgttgtgaa caaggatatg tccagcagtt tgagaagtgt gagggtgagc attgttctca
     4201 gttggcttac catgagtcgc ccaacgagta tgaccgatcc ctgttgtccc ttcagtctta
     4261 tcgccaactt ttgcagacaa ttctgcaatg cgaccaacag ctttcacaag atgagcttgc
     4321 tcaccacccg tcacaaaaat acctgctgaa tcataaccac ggtattctaa tttttcaagt
     4381 ccttgaatca aaatatcagt tgcgtttgta tttcctacaa caccaacgat tccacacata
     4441 catatcacct acgatgtttg tcaacatcgg ctttcatttc tttaaaaatt ggtctagtct
     4501 aatttgtatc taaaaagacc agtgaaatca gtatataaca agtttttatc cttgtcaaca
     4561 ttttaattgg tctagtctaa tttcaaaata cagttccagt catctcaaca caattaaaaa
     4621 aaggctctat aatttctgta gtgggtaaat ccactgtaga gattatggag gctttttgag
     4681 tatatacaaa aagtcccata tgacctataa tgaaaagcaa ccacactcac attagaaaga
     4741 ctcatatgga acaactttat cataccacag aactaattgg aatcaaagac aaaaacatca
     4801 aaattctctt cattttgaaa catcagaccc atctagaaat cagggcaaag ctagattacg
     4861 acagccctgg ttgtcctcat tgccaaggaa agtgtatcaa gtacgacttt caaaagtcgt
     4921 caaagattcc gattttggat tgccagggct ttccaaccct ccttttgctt aagaaacggc
     4981 ggtttcaatg caaatcttgc caaaaagtga ctgtagctga gacagctcta gtcaagaaaa
     5041 actgtcagat ttccctacct atttgggaga aagtgacaca actccataca gaaaacatga
     5101 ctaatatagc catcgctagg agattacata tctccgtatc ggtcgtccag aggaaactgg
     5161 ctcagtttca atttgagcat gattttacga aattaccaaa agtcttgagt tgggatgaat
     5221 ttagtcggaa caagggaaaa ctcgccttta ttgctcagaa ttttgagacg agatcgattg
     5281 tgaccattct tgacaacaac cgccaaacca ccatcaagaa ctatttctac aagtatccta
     5341 gggcggtcag agaaactgtc gaggtcgtaa cagtggacat gtcgggtagc tatattccca
     5401 tcaccaaaaa actatttcca agagctaaaa ttgtccttga tagatttcac attatccaac
     5461 acctgagccg tgctatgatg acgactagaa ttgatattat gaagactttc gatacgcgtt
     5521 ccctacctta tcgatccatg aaaaatcact ggcgtattct ccaaaaagat agtcgaaaac
     5581 tgtctctgaa tcgcttcttt tctcgcactt ttgggcaaac agtaacaccg agagaggttg
     5641 tccagaagac attgaatttc tctgaggaac tgaaattcta ttacgaactc tatcagatcc
     5701 ttctcttcca tttccaagag atgaactcga aatatttctt cgagcttcta gaagacaatc
     5761 tggatcttgt caatcctgcc tttaaaactg tttttaaaac ttttctaaag tataaaacgt
     5821 acatcacgaa cgccatggag cttccatatt ccaacgctaa gctggaagcg accaataaac
     5881 tcatcaaaga catcaagagg caagcgttcg gctttaggaa ctttacgaac tttaaaacaa
     5941 agatatatat tgctttaaac atcaaaaatg agagaacgaa tttcgtcctc tctaggtgtt
     6001 agcttttcgt ctacccacta cagttgacaa agagcctaaa aaaagctgat tctactgaac
     6061 cagccctgtc ttaataaaac caaattctga aagtggacta atccgaaaag tcagtctacc
     6121 aacaatagcc tcactagaaa tcaaaccaaa actacgacta tcctgttgat tcgttcgtac
     6181 atcattcaac accaaatagt gcccctctgg aatgacgttg tactttccat ttgtcaaggt
     6241 ttccaatgta aagtcatctg tataactttc ttgtgaatga ggcgttttta aatagttctc
     6301 tgtaataata atatcattac gatagagaac atcgtccata taggtaaccg tctcgttttc
     6361 caatgcaatc acacgactca catactcttt tccctcatgg ttatacaaaa taaaatctcc
     6421 atgaatcggg cggacatttc gcactgtcat gataaagtca ttttccttca aataactatt
     6481 tgccatctca ggggtaatcg tgactggctc aagtaaccaa aatcgtaggc caatgattcc
     6541 caaaatcaat aagaccaata aactgatttg ttttattaaa tctcttttta ccataatctc
     6601 tccatttcac tagtattata ccacaattta gagccattag tgtctgagat gctggtaaat
     6661 ctcttcagca ataacttgat ttcctaacgg tgtcaaatga agaccatcgc tatagtattc
     6721 aagatgtccc tccgtcacag tgtaaaggtt aataactggt aaaccaagtt cgtttccgat
     6781 agctgggata attttttgta attcatcttc aataatttga ttattcaatc caaaacgact
     6841 ggttggtacg taaggcggga tgactaaata aacatccggc tggcttggta aatccatata
     6901 ctgctctgcc atttccctat actctcgaat aaacctctcc ctattccaat atggtcctcg
     6961 gctatcgtta ctaccaatca tgatgatgac aatatctgct tcactctcaa gtgaaagttg
     7021 ggcattcttc tctttaaagt aaggaaaatc tgcagttgac tgaagtgaac gcccactcaa
     7081 tccataattt gaaacttgat agtgatgccc aagtttctca gctagaatac aaggataggc
     7141 atctttttcc cgattctcca agccatatcc ataagtcaaa ctatctccga ctgttgcaac
     7201 ttttacggcc ccacttccaa cagttaggac ctcaggatac attatatctg acacacgaac
     7261 tcctcaattt ttcttatttt ctattgtatc atgttgaaaa aaataaataa acttccgaat
     7321 ttgtgatata attaacttat gaaaaaacaa gttattatta taggcggagg aattgttggc
     7381 tctactgtag ccttttatct ctcccaagaa gaacaagtca atcttactct cattgattca
     7441 ggtattggaa cagccacacg agctgccgca ggtatcatct gcccatggat ggctcagaaa
     7501 aaaaataaag actggtataa gctgacttcc gatggtgctg ttttctaccg tcaattggta
     7561 gccgatttgg aaaaatcggg tgctactgaa attccattca aacagactgg aaccatcggc
     7621 ctaaaaagta agcctgaact acttgataaa atacaaaaaa ttgccgagga acgtcgcgta
     7681 gatactccga ctattggcga aattactccg cttataggct cggaaattaa ccagtacctg
     7741 ccactactta agccagacta ttttggtatc catttagaag gtggcggtcg tattgatggt
     7801 gggcgtttga ttgatattct tcaggaacaa tttttgaaaa atggtggtac acgtatctac
     7861 ggacaagcaa aactgctcga caaccatact gtcgaagtag ataatcaagt gcttactgct
     7921 gaccatatta tcttagccac aggggcctgg ttgccacata ttcttgaacc tcttggttat
     7981 caagtggatg ttcgaccaca aaaaggacaa ttgttcgaat tagatacaga attcgatacc
     8041 gacaattggc cagtttgtat gccttacgga gagattgaca tcctaccatt tgaaaatggg
     8101 aaaatcatta ttggtgcaac tcatgaagat gacatgggct acgatttaga actagaccct
     8161 gaaaaaatcc aagccatgca tgataaaata gctgaattca tgcctgattt ggcaaactat
     8221 cctgtagcca gaactcgcat tggaacgcgc gcctatactt caacttattc accattctac
     8281 ggaaatattg aggatatgcc taatgtctgg gtagctagtg gtctgggctc caccggtctg
     8341 acaaacggtc caattatcgg ttggcaaatt gctaaagaaa ttctcaatca agaaacaaac
     8401 tttgaccgta caccatattc tccaaataga tatattctca aaaaataaga tacgatacgt
     8461 ttagattcta ctaactcttt aaatgtagat actaagacta gttgttatct aagcaataaa
     8521 tcatgaccac ccgtcaaacg ggtggtttga acaa
//
LOCUS       DBNFEG010000086         8543 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.086, whole genome shotgun sequence.
ACCESSION   DBNFEG010000086 DBNFEG010000000
VERSION     DBNFEG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8543
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.086"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(549..1748)
                     /locus_tag="AC1PNG_001297"
     CDS             complement(549..1748)
                     /locus_tag="AC1PNG_001297"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIP6701802.1"
                     /translation="MAKLTVKDVELKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYILEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQEVAFIAGATRGAELE
                     AAINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAH
                     ASNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLE
                     KADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANA
                     FAGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQA
                     GTIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPG
                     LAALTEK"
     gene            complement(2008..3018)
                     /gene="gap"
                     /locus_tag="AC1PNG_001298"
     CDS             complement(2008..3018)
                     /gene="gap"
                     /locus_tag="AC1PNG_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIP6701803.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKDGGFEVNGKFVKVSAEREPGNIDWATDGVDIVLEATGFFAS
                     KEKAEQHIHANGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALHDAFGVQKGLMTTIHGYTGDQMVLDGPHRGGDLRRARAAAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVATLDKKVTAEEVNAAMKAAATESFGYTED
                     QIVSSDIVGISFGSLFDATQTKVIEVDGEQLVKVVSWYDNEMSYTAQLVRTLEYFAKI
                     AK"
     gene            complement(3385..5376)
                     /locus_tag="AC1PNG_001299"
     CDS             complement(3385..5376)
                     /locus_tag="AC1PNG_001299"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HIP6701804.1"
                     /translation="MRKKLKFSLVAVACATFLTACGTSNSSAPKAGEATPQTDFHSYI
                     NEQWLKDTKLGAGESQIDNLSQLSEKTQEAIQEMIDQLDMDYSKLQAGSDEKKLIDFY
                     RLAADFDTRNKLGLEPIKPYLEEIKKASTMEELNKILVALYGKNIRNLLHINVTQDIK
                     DSNKNTIYVDPHKLSFPKENYDGTDEFSLKNQKAFKAYLQEVFSLAGESSKEASSKAD
                     LLFNLEKEMAAVQRPKEEANNFEAMYNEKTWDEVKALVPNLPIAELVSELGLQNAPTT
                     VVSEPDALKKLNELYQEKNLDALKALLQYRVIAHYNDYLSSNLIEAKAKYDGVATGAV
                     DTPSQEEVAQAAIDANFSDMIGKVYVKHHFSEESKANILSMVQEIKATYAERIKAVQW
                     MSEETKMRALKKLDTLVVKIGYPDQWKDYSQLAIQSKEEGGSLLSNFDMVKTLELREE
                     MGQFGKPFDKSQFGMPSHTVNAYYSPTNNEIVFPAGILQAPLYDPKASPEENLGGIGA
                     VIGHEISHAFDKAGSQFDEKGNLVNWWKPEDLEKFNKKVQQAADIYSKLEVAPGYYVN
                     GEISTGEIMADLGGLTVAIDIAQKKGYDTKKVFESYGKAWREVTTKEFAIANISDEHP
                     PAKYRVNNIVNQIDQFYTDFNVKEGDPMYVKPEDRLRVW"
     gene            complement(6358..8439)
                     /gene="fusA"
                     /locus_tag="AC1PNG_001300"
     CDS             complement(6358..8439)
                     /gene="fusA"
                     /locus_tag="AC1PNG_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIP6701805.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHERLQANAH
                     PIQLPIGSEDEFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLEQAEEYREKLVEAV
                     AETDEELMMKYLEGEEITNEELKAAIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGVNPDTDAEEERHASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVLNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESIHVPEPVIQLMVEPKSKADQDKMGIALSKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPIVDVKAKLY
                     DGSYHDVDSSETAFKVAASLALKEAAKSAQPTILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEARGNTQIVRAYVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     DEIIKKNGGNA"
BASE COUNT         2380 a         1877 c         1641 g         2645 t
ORIGIN      
        1 tacaaaaaaa taagtatcct atagttgatt ataatacgag acaagaagcc acaagcataa
       61 gtcgagctta aaaaggtgag tttacgaagt aatactcaat gaaaatcaaa aaaatgatta
      121 cacaacaaaa tttttcctcc gagccgacac ttcagagcgt aaattgcatt taaaaagaga
      181 ccggagctca agccccagcc tcatttcgtc gcaatttttt acttttcagt aagagctgca
      241 agtcctggaa gcacttttca ccttgctaga ctttctaact cgcttagctt cgtagaaact
      301 ctacgcgatg cacaaaatgc aacggaaatc cgttgcattt tagcctttac ctaggctgat
      361 tttctaaact cactacgttc acagaaaatc tagcctttgt actagaaaag agttggtctc
      421 ccaactcttt tcatcgttca tctttcatac gcagtagctg aatggcagtt cactttgcta
      481 cgcttgtgac caacctattc agccttgcgg gggtgcgtct acggaatcat agattcctga
      541 cttaccgcct atttttcagt caaagctgca agtcctggaa gcactttacc ttcgaggagt
      601 tccattgatg caccaccacc agttgagatc catgagaact tgtctgcacg gccaaggttg
      661 atcgcagctg ccgctgagtc accaccaccg atgattgatt tcacgcctgg ttgtttcacg
      721 atagcgtcca ttacaccgat tgtaccagct tggaagtctg ggttttcaaa tacacccata
      781 ggtccgttcc aaacaactgt ttttgcacca gtcaacgctt cgtcaaactt agcgattgac
      841 ttaggaccga tgtcaagacc aaggaagcct tctgaaactg cttcaccttc agtgtcgcga
      901 acttcagtgt atccagcaaa tgcatttgct tcttttgagt caactggcaa gatcaattta
      961 ccattcgctt tctcaagaag cgcctttgcc acatccaatt tatcttcttc tacaagtgag
     1021 ttaccaattt cgataccttg agctttgtag aatgtgtaag tcataccacc accgataaga
     1081 actttgtctg ctttttcaag aaggttctcg ataacaccaa tcttatcaga tacttttgaa
     1141 ccaccaagga ttgccacaaa tggacgctct ggagtttcaa cagcttcttg gatgtatgca
     1201 atttcgtttt ccaaaaggaa accagctact gctttttcta cgtttgctga gataccaacg
     1261 tttgatgcgt gtgcacggtg tgcagtacca aatgcatcgt taacgaagat accatcacca
     1321 agtgaagccc agtatttacc aagttcttcg tcgtttttag attctttctt accatcaacg
     1381 tcttcgaaac gagtgttttc aacaaggaga acttgtccat cttccaaagc attgatagcc
     1441 gcttccaatt cagcaccacg agtagcacca gcgatgaaag caacttcttg acccaattta
     1501 gctgccaagt cagctgctac aggagccaat gatttacctt ctttgtcagc ttcttctttc
     1561 acacgaccaa ggtgagagaa aagaattgca cgtccacctt gctcaagaat atacttgata
     1621 gttggaagag ctgctgtaat acggttatcg ttagtgataa cgccatcttt caaaggcacg
     1681 ttgaagtcca cacgaacaag aacttttttg cctttcaatt ctacatcttt aacagtcaat
     1741 tttgccatta gataagaact ccttaaatta tatttacttt accattgtat catattttct
     1801 gtattttttc ttgcataaag atattttttt cacaaatgtt atgaaatcta aaactatcac
     1861 ctcgtaatca gatttttcaa attctaaaat cgccaaatca gaaacaactc atctcattca
     1921 tatttttagc ataagaaaac agccatattc cagcaatccg aaaatacaaa aaaaagaggc
     1981 cgaagcctct tcgattttca ctaaggatta tttagcgatt tttgcgaagt actcaagagt
     2041 acgaacaagt tgtgcagtgt aagacatttc gttgtcgtac catgaaacaa ctttaaccaa
     2101 ttgctcgcca tcaacttcga taactttagt ttgagttgca tcaaacaatg aaccgaatga
     2161 gatacctacg atatctgaag atacgatttg gtcttcagtg taaccaaatg attcagtagc
     2221 agcagctttc atagcagcat ttacttcttc agcagttact ttcttgtcaa gagttgcaac
     2281 caattcagtt acagaacctg ttggaactgg aacacgttgt gcagcaccgt caagtttacc
     2341 gttcaattct gggattacca agccgatagc tttagcagca ccagttgagt taggaacgat
     2401 gtttgcagca gcagcacgtg cacgacgaag gtcacctcca cggtgtggtc cgtcaagaac
     2461 catttggtca ccagtgtaac cgtggattgt agtcatcaaa cctttttgaa cgccaaatgc
     2521 atcgtgaaga gctttagcca ttggtgccaa acagtttgta gtacatgaag cacctgagat
     2581 aactgtttca gtaccatcaa ggatgtcgtg gttagtgttg aaaacaacag tcttaacatc
     2641 gttaccacca ggagcagtga taacaacttt tttagcaccg ttagcgtgga tgtgttgctc
     2701 agctttttct ttagaagcaa agaaacctgt agcttccaaa acgatatcta cgccatcagt
     2761 agcccagtcg atgtttcctg gctcacgctc agcagaaact ttaacgaatt taccgttaac
     2821 ttcgaaacca ccgtctttaa cttcaacagt accgtcgaaa cgaccttgag ttgtgtcata
     2881 tttcaacaag tgtgcaagca ttactggatc tgtaaggtcg ttgatacgag taacttcaac
     2941 accttctacg ttttggatac gacggaaagc aagacgaccg atacgtccga aaccgttaat
     3001 accaacttta actaccatga atgatttcct ccttatgaaa atcgagttct ttattttaaa
     3061 aggcaagcct tttaatcctt ttgtgaaaat attaacttgt aagcctacaa gcatcttttc
     3121 aacgttttta ttatataact atttaccgaa aattgcaact gcttaccggt tcatcttatg
     3181 taaaaaatag aaggccaaaa aataaaagtt aaaaatcata gccatgcgtt ttctcgggtg
     3241 gttatgactg gttaaatact taacttaata gcattgccat acctcgacac attttttacc
     3301 gattcttggt ttaccgctac ttctattttt tacctaaaaa ggtgtctacc gcccattttg
     3361 gaccgcagac acctctataa tcttttacca cactcgtagg cggtcttctg gtttcacata
     3421 cattggatcc ccttctttga cattgaagtc tgtatagaat tgatcaattt ggttgacaat
     3481 gttgttaacc cgatatttag ctggtggatg ttcgtcgctg atgttggcaa tagcaaattc
     3541 tttggtggtg acttcacgcc atgctttacc ataagactcg aaaactttct tcgtatcgta
     3601 gccttttttc tgagcaatat caatggcaac cgttaaacca ccgagatccg ccatgatttc
     3661 accggttgaa atttcgccat tgacatagta gcccggagcc acttccaatt tagaatagat
     3721 atctgctgcc tgttgaacct ttttattaaa tttttcaagg tcttctggtt tccaccagtt
     3781 gacaaggttc cccttttcat caaattgaga accagcctta tcaaaggcat gggaaatttc
     3841 atgaccgata acagccccga ttccacctag attttcttcg ggactagctt ttggatcgta
     3901 gagaggagcc tgtaaaatcc ccgctggaaa gacaatttcg ttgttagttg ggctgtaata
     3961 ggcattcacc gtgtgagaag gcatgccgaa ctgcgactta tcaaaaggct tgccaaattg
     4021 ccccatctct tcacgcaatt caagtgtttt taccatatcg aagttagaaa gaagacttcc
     4081 cccttcttcc ttggattgga tagcaagttg tgagtaatcc ttccactggt ctggatagcc
     4141 gattttaaca accaatgtat ctagtttttt caaggcccgc atcttggttt cttcagacat
     4201 ccactgcact gctttgattc gctctgcata agtcgcttta atctcttgaa ccatagatag
     4261 gatattagcc ttggattctt ctgaaaaatg gtgtttaacg taaactttgc caatcatatc
     4321 ggagaaattt gcatcaatgg ccgcttgagc aacttcttcc tgactcggag tatctaccgc
     4381 accagttgct acgccgtcgt atttcgcttt tgcctcgatt aaattgcttg acagataatc
     4441 attgtaatgg gcaatcacac gatattggag cagggcttta agagcgtcta aattcttttc
     4501 ttgatagagt tcatttaatt tttttagagc atctggttct gatacgactg tcgttggagc
     4561 attctgaagt cctaactcag aaaccaactc agcaatcggt aggttgggaa ccagtgcttt
     4621 gacctcatcc caagtcttct cgttatacat ggcttcaaag ttattggctt cttcttttgg
     4681 ccgttgcact gcagccatct ctttttccaa attaaagagg aggtctgcct tagaagaggc
     4741 ttcttttgag gattctcctg ccaagctaaa cacttcctgc agataggctt taaaggcttt
     4801 ttggtttttc agtgaaaatt catctgtgcc atcataattt tcttttggaa aacttaactt
     4861 gtgagggtca acgtagatgg tgtttttgtt actgtctttt atatcttgag taacgtttat
     4921 atgaagcaaa ttgcggatgt ttttgccgta tagggctacc agaatcttgt tgagttcttc
     4981 catggtagag gcttttttaa tctcctctaa ataaggcttg ataggctcta accccaattt
     5041 gttgcgagta tcaaaatcgg ctgccaatcg gtaaaaatca ataagttttt tctcatcact
     5101 accagcttgc agcttggaat agtccatatc cagctggtca atcatctcct ggattgcttc
     5161 ttgcgttttc tcagagagtt gactcagatt atctatttgc gattcaccgg cacctaattt
     5221 tgtatctttc aaccattgct cattgatata agaatgaaaa tccgtctgtg gtgttgcttc
     5281 acctgctttg ggagccgagg aattacttgt accacaggct gtcaaaaatg tcgcacaggc
     5341 gactgctact aatgaaaatt ttaatttttt tctcatgatt tctgtccttt ctttttattg
     5401 ttaatgtgct tagtaccact atttcatcac catacaagca tcctccttta gtaattagcg
     5461 gtcctaaact actctttcat cgatctacct tataaggatt ctaacattta tatctgcttt
     5521 tcttctgtag cctatctatt aattctctta ttttttgtaa ccattcccaa acaaagtaat
     5581 tctaaatgat ttaaaacatt tattttagca aattgagtga gagtaggcaa gaactcgaac
     5641 ggtcaaaaga gttcagcaac aattcctaat ttcaagttat taagctaaaa caatctattc
     5701 acagaccgtt ttaatccgtt tatttcacag tagccagacc tagaagtcac tgttgagttc
     5761 atcggcaaca ggaatcttat acccaatgaa atcccaaaat agcaaaccct gatcttttgt
     5821 tagccatcca cacctaaatt agggtatttt tttataaatt gtagctttct cattgtatat
     5881 ttccaaaaag gatctgacgg atacctattt tttagtcgtt tagtttgttt ttagcactca
     5941 cttcaaaggt tcagggaacc tttgaaggct agagataggg cgaagcaagt tcgcctcaat
     6001 agctgcaggt tgctagaaca gcctttaggc tgttctaagt tagaaataag acttgcctcg
     6061 cgaatcacct ctgtaaagta cggcaaagcg agttcaaata ctccagtgaa gtgttcgaag
     6121 ttgaaaatag ggaaacgaag tttcatcgct cgtcgaagta atcaaagata aactactgat
     6181 actcaataaa aatcaaaagt agcctaggaa acgaagccga agatagaact ggagttcatc
     6241 aaggcaagtt gacaacggat aattttgatt tccgaagagt atgagtatca cgctcagatg
     6301 tgtgacatat ccgtcgtacc aaaaaacacc tacccgaagg taggtgtaga taggcaatta
     6361 tgcattgcca ccgtttttct taatgatttc atcttgtaca gattttggta catcttcgta
     6421 gtggtcaaat accatcatga aggtaccgcg accttgagtt gctgaacgaa gaacagttgc
     6481 gtaaccgaac atttcagcaa gtggcacata agcacgaacg atttgtgtgt taccacgagc
     6541 ttccataccg tcaacgcgac cacgacgtgc agtaacgtga cccataacgt cacctaggtt
     6601 atcttctggt gcagtgatgg ttacaagcat cattggctca aggatagttg gttgagctga
     6661 tttagcagct tctttaaggg caagagatgc tgccactttg aaggctgttt cagatgagtc
     6721 gacatcgtgg tatgaaccat cgtaaagctt cgctttaacg tcaacgattg ggtaaccagc
     6781 aagaacaccg ttcgccatag attctacgag acctttttca accgctggga tgaattcacg
     6841 tggaaccaca ccaccgacga tagcgttctc gaactcgaaa cctttacctt cttcgtttgg
     6901 tgtgaattcg atccaaacgt caccgaattg acctttacca ccagactggc gtttgaagaa
     6961 accacgagct tgtgtagaag cacggaatgt ttcacggtat gatacttgag gagcacctac
     7021 gtttgcttca accttgaatt cacgacgcat acggtcaaca aggacatcca agtgcaactc
     7081 acccatacca gagataactg tttcaccagt ttcaacgttt gtttcaacgc ggaatgttgg
     7141 atcttcttca gccaatttag aaagggcaat acccatcttg tcttggtcag ctttagactt
     7201 aggctcaacc atcaattgga taactggttc tggaacgtgg attgactcaa ggatgatttt
     7261 tgctttttca tctgtcaatg agtcaccagt tgtagtatct ttcaaaccaa ccgcagcggc
     7321 gatgtcacca gcgtaaacag tttcaatttc ttggcggctg tttgcgtgca tttgaaggat
     7381 acgtccgata cgctcacgtt tacctttaga agtgttcaat acgtatgaac cgctgttaag
     7441 gacacctgag taaacacgga agaatgtcaa acgacctacg aatgggtcag tcataatctt
     7501 gaaggcaaga gctgcaaatg gctcttcatc tgatgcatga cgctcttctt cagcatctgt
     7561 atctgggtta acacctttaa tagcagggat gtcaagtggg cttggaaggt agtcgataac
     7621 cgcatcaagc atcaactgaa cacctttgtt tttgaaggct gaaccacaca atactgggaa
     7681 gaattcaacg ttgatagttg ctttacggat agcagctttc aattcttcgt ttgtgatttc
     7741 ttcaccttca aggtatttca tcatcaattc ttcatcagtt tcagcaactg cttcaaccaa
     7801 tttttcacgg tattcttcag cttgttcaag gtattcagct ggaatatctt cttcaagaat
     7861 atctgtacca aggtcgttag tatagatttc agctttcatc ttgatcaagt cgatgatacc
     7921 acggaactca tcttctgagc cgattggcaa ttggattgga tgagcatttg cttgaagacg
     7981 ctcatgaagt gtgcttactg aataaaggaa gtcagcaccg attttatcca ttttgttagc
     8041 gaatacgata cgtggaacac cgtattcagt tgcttgacgc catactgttt cagtttgtgg
     8101 ctcaacacct gattgtgagt caagaacggt taccgcacca tcaagaacgc gaagtgaacg
     8161 ttgtacttcg attgtaaagt ccacgtgtcc tggtgtgtcg atgatgttta cacggtggtt
     8221 gttccattga gctgttgtcg cagcagatgt gatagtgata ccacgctctt gctcttgctc
     8281 catccagtcc atttgcgatg caccttcgtg agtttcaccg attttgtgga ttttaccagt
     8341 gtagtaaaga atacgctcag ttgtcgttgt tttacccgcg tcaacgtgcg ccatgatacc
     8401 gatattacga gttttttcta atgaaaattc gcgtgccatg aggtttgttc tcctatattt
     8461 ttagatttct attctgatta taccatattt taagaaaagc gagtgggcaa atcccacccg
     8521 cttaagttct tactaatggt gtg
//
LOCUS       DBNFEG010000087         8518 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.087, whole genome shotgun sequence.
ACCESSION   DBNFEG010000087 DBNFEG010000000
VERSION     DBNFEG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8518
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.087"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..337
                     /locus_tag="AC1PNG_001301"
     CDS             <1..337
                     /locus_tag="AC1PNG_001301"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIP6701806.1"
                     /translation="LDEKSYQLDNLFFISVANSLYFGGGIMIWPTASAKKKEVDIVYF
                     KNGNFYQRLQSLLALLTKRHESSHTIQHLTGVDVVLKSKEKLLLQIDGETCTANEVTL
                     TYQERSMYL"
     gene            347..550
                     /locus_tag="AC1PNG_001302"
     CDS             347..550
                     /locus_tag="AC1PNG_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HIP6701807.1"
                     /translation="MYQLGSFVEMKKPHACVIKSTGKKANKWEVIRLGADIKIRCTNC
                     DHVVMMSRHDFEQKMKQVLPSEA"
     gene            654..1019
                     /locus_tag="AC1PNG_001303"
     CDS             654..1019
                     /locus_tag="AC1PNG_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6701808.1"
                     /translation="MNQLAQQIRILRTAKNLSQDELAEKLYISRQAVSKWENGEATPD
                     IDKLVQLAEIFGVSLDYLVLGKEPEKEIVVEQRGKMNGWEFLSEESKRPVENKEIFLF
                     ILSMAICIFVVWILTMITK"
     gene            1131..2246
                     /gene="ychF"
                     /locus_tag="AC1PNG_001304"
     CDS             1131..2246
                     /gene="ychF"
                     /locus_tag="AC1PNG_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIP6701809.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KDSVAEFAVLEKIKPVLEDGKSARTVDFTDEEQKIVKQLFLLTTKPVLYVANVDEDKV
                     ADPESISYIQKIRDFAATENAEVVVISARAEEEISELDDEDKGEFLEALGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQCAGIIHSDFEKGFIRAVTMSYDD
                     LMTYGSEKAVKEAGRLREEGKEYVVQDGDIMEFRFNV"
     gene            2404..2973
                     /gene="pth"
                     /locus_tag="AC1PNG_001305"
     CDS             2404..2973
                     /gene="pth"
                     /locus_tag="AC1PNG_001305"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIP6701810.1"
                     /translation="MTRLIIGLGNPGDRYFETKHNVGFMLLDKIAKRENVTFNHDKIF
                     QADIGTTFIDGEKIYLVKPTTFMNESGKAVHALMAYYGLDATDILVAYDDLDMAVGKI
                     RFRQKGSAGGHNGIKSIVKHIGTQEFDRIKIGIGRPKGKMSVVNHVLSGFDTEDRIEI
                     DLALDKLDKAVNVYLEEDDFDTVMRKFNG"
     gene            2973..6467
                     /gene="mfd"
                     /locus_tag="AC1PNG_001306"
     CDS             2973..6467
                     /gene="mfd"
                     /locus_tag="AC1PNG_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIP6701811.1"
                     /translation="MNILDLLHKNKQINQWQSGLNKSTRQLLLGLTGTSKSLVMATAY
                     DSMAEKIMIVTATQNDAEKLVADLVAIIGSENVYNFFTDDSPIAEFVFASKERTQSRI
                     DSLNFLTDSTSSGILVASMAACRVLLPSPETYKGSKIKLEVGQEIEVDKLVNNLVNIG
                     YKKVSRVLTQGEFSQRGDILDIFDMQAETPYRIEFFGDEIDGIRIFDVDSQKSLENLD
                     EISISPASDIILSSEDYSRASQYIQTAIEQSTLEEQQSYLREVLADMQTEYRHPDLRK
                     FLSCIYEQSWTLLDYLPKSSPLFLDDFHKISDKQAQFEKEIADLLTDDLQKGKTVSSL
                     KYFASTYAELRKYKPATFFSSFQKGLGNVKFDALYQFTQHPMQEFFHQIPLLKDELTR
                     YAKSNNTVVIQASSDVSLQTLQKTLQEYDIHLPVHAADKLVEGQQQVTIGQLASGFHL
                     MDEKLVFITEKEIFNKKMKRKTRRTNISNAERIKDYSELAVGDYVVHHVHGIGQYLGI
                     ETIEISGIHRDYLTVQYQNFDRISIPVEQIDLLSKYLASDGKAPKVNKLNDGRFQRTK
                     QKVQKQVEDIADDLIKLYAERSQLKGFAFSPDDENQIEFDNYFTHVETDDQLRSIDEI
                     KQDMEKDSPMDRLLVGDVGFGKTEVAMRAAFKAVNDGKQVAILVPTTVLAQQHYANFQ
                     ERFAEFPVNVDVMSRFKTKAEQEKTLEKLKKGQVDILIGTHRLLSKDVVFADLGLLVI
                     DEEQRFGVKHKERLKELKKKIDVLTLTATPIPRTLQMSMLGIRDLSVIETPPTNRYPV
                     QTYVMETNPSVIRDAMLREIDRGGQVYYLYNKVDTIEQKVSELKELVPEATIGYVHGQ
                     MSEIQLENTLYAFVEGEYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSSRIAYAYLMYRPDKSLTEVAEKRLEAIKGFTELGSGFKIAMQDLSIRGAGNILG
                     AAQSGFIDSVGYEMYSQLLEQAILEKQGKATQRQKSNSEVNLQIDAYLPSDYIGDQRQ
                     KIEIYKRIKNIDSRVNYQELQEELIDRFGEYPDVVAYLLEIGLLKSFLDQVFCHTVLR
                     RQHQVTVTFEPMACQIFLTQDYFEALSVTNLKAQITENKGKLAVVFNIQQKKEYEILE
                     ELISFAEKLKEIKARKAE"
     gene            6517..6789
                     /locus_tag="AC1PNG_001307"
     CDS             6517..6789
                     /locus_tag="AC1PNG_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIP6701812.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIQFGNKLLTVKVLEMKDSTKKEDALKMYEIISEKRIEKDEEI"
     gene            6776..7144
                     /locus_tag="AC1PNG_001308"
     CDS             6776..7144
                     /locus_tag="AC1PNG_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIP6701813.1"
                     /translation="MKKSKILQLNNAFIQSERKKTQHQLAERQQKNRFMGAILILVIF
                     LFMLPAYNLVGTYTNIQQQEKKLAELEKNYEELTKEQKQEAEMVAKLKNEEYAAKYVR
                     AKYQYSKEGEFVYNIPGLPK"
     gene            7141..7254
                     /locus_tag="AC1PNG_001309"
     CDS             7141..7254
                     /locus_tag="AC1PNG_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0009 family protein"
                     /protein_id="HIP6701814.1"
                     /translation="MTESIFKIVEQFLQHSDEKLEELNQKNRELKLEENES"
     gene            7267..>8518
                     /locus_tag="AC1PNG_001310"
     CDS             7267..>8518
                     /locus_tag="AC1PNG_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIP6701815.1"
                     /translation="MQKKLLVWLLPVLFGWQVVDSTEIPFELTAQEEYELTHTIYDQY
                     FQTIPQNPNVFQTENLYSDEELTIAGGQLQPNQHFSITDVLVNSKKELVFQIDDKGYI
                     LASRHLLFDDVIVTETTVEQTYWTKKGFTLLTSPIANEATEIKNDLQPYQAVMVSKIV
                     TTSLGDFAYVTDKGWIAVNNLSKTDNRVEAVQELLTNKYSKDTIGIYVKQLSTGQTAG
                     VNQEKLFYSASIAKLPILYYVQEQLNAGYIDLTTKVKYTAESISFPGAYVAGGSGSLS
                     KTPDNKDYSVEELINKTAKESDNVASNLLSYYVANKFDSNFYQVITAKTGSEWSMVTR
                     ETSAEIAGKMMESLYIQNGYVLESLLSTQFDNQRISKDISVPVAHKIGDADDVKHDVA
                     IVYAGSPFVLSIFTDKSNYDEITQI"
BASE COUNT         2851 a         1443 c         1764 g         2460 t
ORIGIN      
        1 tcttgatgaa aaatcttatc agttagataa tctctttttt atttctgtag caaatagtct
       61 ttattttggt ggaggaatca tgatatggcc aacagcaagt gctaaaaaga aggaagtaga
      121 tattgtttac ttcaaaaatg gaaatttcta ccaacgtcta caatcattgt tagccttatt
      181 aacgaagagg catgaatctt ctcatacaat tcagcattta acaggggtag atgtagtttt
      241 aaaatcaaaa gaaaaattat tattgcaaat agatggagag acatgcactg caaatgaggt
      301 aacgttaacc tatcaggaaa gaagtatgta tctttaagga ggaagtatgt atcaattagg
      361 atcatttgtc gaaatgaaaa agcctcatgc ctgtgtcatc aaatcgaccg gaaagaaggc
      421 aaataagtgg gaggttatcc gtctaggagc ggatattaaa atccgttgta ccaactgtga
      481 ccatgtagtc atgatgagcc ggcatgattt tgaacaaaaa atgaaacaag ttctaccaag
      541 tgaagcctag ttgacagttt gcaactgctg gttgcgaata tttcctaaaa agtgctagtc
      601 aactgttcct ttctaaacta taatagactt agaaaaaaga ggaggaaatg atcatgaatc
      661 agttagcaca gcaaattcga attttacgca cagccaagaa cctatcccaa gatgagttgg
      721 cagagaaact ctatatttcc cgtcaggctg tttccaagtg ggaaaatggc gaagcaacgc
      781 cagatattga caaactggtt cagctagcag aaatctttgg tgtcagtctg gattatctgg
      841 ttttaggaaa agaacctgag aaggaaattg tggtggaaca acgaggaaaa atgaatggtt
      901 gggaattttt aagtgaagaa tccaagcgac cagttgaaaa taaagaaatt tttctgttta
      961 tcctctctat ggccatctgt atttttgtag tttggatctt gaccatgatt acaaaataat
     1021 ggattattta ttcagcatta tttttaacga agcttgctaa tcggcaagct ttttctctac
     1081 ttttctgcta taatagtcat gattgaattt ttaattggag agtaaaaaac atggctttaa
     1141 cagcaggaat cgtcggtttg ccaaacgttg gtaaatcaac tctatttaac gcaattacca
     1201 aggcaggagc agaagctgca aactaccctt tcgcgaccat tgatccaaac gtcggcatgg
     1261 tggaagtgcc cgatgagcgt ttgcagaaat tgacagaact catcacccct aaaaagacag
     1321 tgccaacgac ttttgaattt actgatattg ccggtatcgt aaaaggtgct tctaaaggtg
     1381 aaggacttgg aaataaattc ttggccaata tccgcgaagt tgatgcgatt gtccacgttg
     1441 tgcgtgcctt tgacgatgaa aatgttatgc gtgaacaagg ccgtgaagac gcctttgttg
     1501 atcctatcgc agatattgat actattaacc tagaattgat tttggcggac ctagagagca
     1561 tcaacaagcg ttatgcgcgt gtagaaaaaa tggcccgtac gcaaaaagac aaggattctg
     1621 tcgcagaatt tgcagttctt gaaaaaatca aacctgtcct agaagacgga aaatctgctc
     1681 gtacagttga cttcacagat gaagaacaaa aaatcgttaa gcaactcttc ctcttgacca
     1741 ctaaaccagt cctctatgtt gccaacgtcg atgaagacaa ggtagcagat cctgagtcta
     1801 tcagttatat tcagaaaatc cgtgactttg cggcaacaga aaatgcagaa gtggtggtga
     1861 tttctgcgcg tgctgaagaa gaaatttcag aactcgacga tgaagacaag ggcgagtttt
     1921 tagaagcact cggattaaca gaatctggcg tggacaaatt aacacgtgca gcctatcact
     1981 tgcttggtct aggcacctac tttacagcag gggagaagga ggttcgtgct tggaccttca
     2041 aacgtggtat gaaagctcct cagtgtgcag gcattatcca ttcagacttc gaaaaaggtt
     2101 ttatccgtgc cgttaccatg tcatacgatg atttaatgac ctacggctct gagaaggctg
     2161 ttaaagaagc aggacgcctt cgtgaagaag gaaaagagta cgtggttcaa gatggggaca
     2221 tcatggagtt ccgctttaat gtttgattgc taaactgtcc ttattcttcg ttgttttacc
     2281 ttgccatacc atcagtattg tctgcggttt caacgcctag actaagaaca gtttatcgca
     2341 tcaaagagga tgattacttg gttggaaagc tttttccagc cttttggtat ttaaggagaa
     2401 aaaatgacac gattgatcat cggtctggga aatcccggag accgctattt tgaaacaaaa
     2461 cataacgttg gttttatgct gctggataag attgctaagc gtgaaaatgt gacctttaat
     2521 cacgataaga ttttccaagc tgacattggc acaaccttta ttgacggcga aaaaatctat
     2581 ctagtaaagc caacgacctt catgaacgaa tcaggtaagg ctgttcatgc cctgatggct
     2641 tattatggtc tggatgcaac tgatatttta gtagcttatg atgacttaga catggctgta
     2701 ggaaaaattc gtttccgtca aaaaggctca gctggtggcc acaatggtat caaatccatt
     2761 gtcaagcata ttggaacaca ggaatttgac cgtatcaaga ttggtattgg gcgtcctaaa
     2821 ggaaaaatga gcgttgtcaa ccacgtctta tcaggatttg atacagaaga ccgtattgaa
     2881 atcgacttag cactagataa acttgacaag gctgtcaacg tatatctcga agaagatgat
     2941 tttgatacag taatgagaaa gtttaacgga taatgaatat actcgattta cttcacaaga
     3001 acaagcaaat caatcagtgg cagtctggct taaacaagtc aactcgccaa ctattattag
     3061 gactcactgg aaccagtaag tcattagtca tggcgactgc ctatgatagt atggctgaga
     3121 aaataatgat tgtgactgct actcaaaatg atgctgagaa attagttgca gatttagtag
     3181 ccattattgg aagtgagaat gtttacaact tttttacaga tgatagtcct attgccgagt
     3241 ttgtttttgc atcaaaagaa cggactcaat caaggattga cagtttaaat ttcttgacag
     3301 attctactag ttcaggaatt ttagttgcta gtatggctgc ttgtcgggtc ttattgccaa
     3361 gtcctgagac ttataaaggt tctaaaataa agcttgaagt tggtcaagaa atagaagttg
     3421 acaaacttgt aaataatctt gtcaacatcg gctacaaaaa agtgtctcgt gtattaaccc
     3481 agggagaatt tagccaacga ggtgatattc tagatatttt tgacatgcaa gcagaaactc
     3541 cttaccgaat agaatttttt ggagatgaga ttgatggcat tcgtattttt gatgttgaca
     3601 gtcaaaaatc cttagaaaat ctagacgaaa tctcgatctc tccagcttcc gatatcattt
     3661 tgtcttcgga agactatagt agagctagcc aatacattca gacagctatt gaacaatcga
     3721 ccctagagga gcaacagtct tacctacgag aagtcttagc tgatatgcag acggagtacc
     3781 ggcatccaga tttgcgtaaa ttcttgtcct gtatctatga acaatcatgg acattgttgg
     3841 attacttgcc aaagagttct cccttatttt tggatgattt tcataaaatc tctgataagc
     3901 aggctcaatt tgaaaaagaa attgcagact tgttgacaga tgatttacag aagggtaaaa
     3961 cagtgtcaag tttgaaatat tttgcctcaa cttacgctga attgagaaaa tacaaacctg
     4021 ccaccttctt ttcaagtttt caaaaaggtt taggcaatgt aaagtttgat gctctttatc
     4081 aatttacaca gcatcctatg caggaatttt tccaccagat tccactgtta aaagatgaat
     4141 tgactcggta tgcgaaatca aacaataccg ttgttattca agcgagttct gatgtgagtt
     4201 tacagacttt acagaaaact ttacaagagt atgatattca tcttccagta catgcagcag
     4261 ataagttagt agaaggtcag caacaggtta cgattggtca gttagcttca ggttttcatt
     4321 tgatggatga gaaactagtt ttcatcactg aaaaagagat tttcaataag aaaatgaagc
     4381 gtaagacacg tagaaccaat atttctaacg ctgaacgtat taaggactat agtgaattag
     4441 cagttggtga ctatgttgtt caccatgttc atggtatcgg tcaataccta ggaattgaga
     4501 ctattgaaat ttcaggcatt catcgtgatt atttaacggt tcaatatcaa aacttcgatc
     4561 gcatttctat tcctgtagaa caaattgatc ttctctccaa atacttagcg tccgatggga
     4621 aagctccaaa agttaataaa ctaaacgatg gccggttcca acgaactaag caaaaagttc
     4681 agaagcaagt ggaggatatt gcagatgatt tgattaagct ttatgcggag cggagtcagc
     4741 tgaaaggctt tgccttttca ccagatgatg agaatcagat tgagtttgac aactacttta
     4801 cacatgtgga aaccgacgac caacttcgtt ccattgatga aatcaaacaa gatatggaaa
     4861 aagactctcc aatggatcgt ttgctggtag gagatgtcgg ctttggtaag acagaggtag
     4921 ctatgcgcgc tgctttcaaa gcagtcaatg atggcaagca agtagctatt cttgttccta
     4981 cgactgtttt agcccagcaa cactatgcta atttccagga acgatttgcg gaatttcctg
     5041 tcaatgtgga tgttatgagt cgttttaaaa caaaggcaga gcaggaaaaa acacttgaga
     5101 agttgaaaaa aggccaagtt gacatcttga ttggtaccca tcgccttcta tcaaaagatg
     5161 ttgtttttgc tgatttaggt ttactggtta ttgacgaaga gcaacggttt ggtgtcaagc
     5221 ataaggaacg cttgaaggaa ttgaaaaaga aaatagatgt cctaaccttg actgcaactc
     5281 cgattcctcg gacattgcaa atgtccatgc tgggaatccg cgatttatca gtaattgaga
     5341 ctccaccgac aaatcgctat cctgtacaaa cttatgtgat ggaaacaaat ccttccgtta
     5401 tccgagatgc aatgcttcgt gagatagatc gcggaggtca ggtttactat ctttacaata
     5461 aagttgacac cattgaacag aaagtgtctg aattaaaaga attggttcca gaagctacta
     5521 taggatacgt ccatggacaa atgtcggaga tccaattaga aaatactctc tatgcttttg
     5581 tagagggtga atatgatatt ctagtgacca caacaatcat cgaaacaggt gttgatattc
     5641 caaatgctaa tacactgttt attgaaaatg cagatcatat gggactgtca actctttatc
     5701 aacttagagg acgtgttggc cgttccagtc ggattgccta tgcctatctc atgtaccgtc
     5761 cagacaagtc cttgacagaa gtagctgaaa aacgtttgga ggcaatcaag ggctttacag
     5821 aactaggatc aggtttcaag attgccatgc aagatttgtc cattcgtggt gcgggaaata
     5881 ttttaggtgc tgctcagtca ggttttattg attctgtggg atatgagatg tattcacaat
     5941 tgctggaaca agccatcttg gaaaaacaag gtaaggcaac tcaacgtcag aagagtaatt
     6001 cagaggtcaa tttacagatt gatgcctatc taccaagtga ttatattggt gaccagcgac
     6061 aaaaaattga aatttacaag cgtataaaga atattgacag tcgtgtcaac tatcaagaat
     6121 tacaggaaga attgattgac cgttttggag agtatcctga tgtagtagcc tacttgcttg
     6181 aaatcggtct attgaagtca tttttagacc aagttttctg tcatacagtt cttagacgac
     6241 aacatcaagt gacagtaaca ttcgaaccta tggcttgtca aattttccta acgcaagatt
     6301 actttgaagc tttatctgtg acaaatttga aagctcagat aacggaaaat aaaggaaaac
     6361 tagctgttgt gtttaacatt caacagaaaa aagaatatga aatattagag gaattgattt
     6421 cttttgccga aaaattaaaa gaaattaaag caaggaaggc agaataaatt tctcaaatac
     6481 cttgaaaaat ggtaaaataa tgaactgagg taaagtatga gactagataa atatttgaaa
     6541 gtatcccgca ttattaagcg tcgaacagtt gcgaaggaag ttgccgataa aggacgaata
     6601 aaagtcaatg ggattttggc gaaatcttca acagatttaa aagtaaatga tcaggttgaa
     6661 atccaatttg gtaataaact actcactgta aaagtacttg aaatgaaaga ttcgactaaa
     6721 aaagaagacg cactgaaaat gtatgaaatt atcagtgaaa aaaggataga aaaagatgaa
     6781 gaaatctaaa attctacaac tgaataatgc ctttattcag tcggagcgta aaaaaactca
     6841 acatcaattg gcagaacgcc aacaaaaaaa tcgttttatg ggtgcaattc tcattctagt
     6901 tatctttctt tttatgttgc ctgcctataa tcttgttggg acttacacta atattcagca
     6961 gcaggaaaag aaacttgctg aattggaaaa aaattatgaa gaactgacca aagaacagaa
     7021 gcaggaagca gagatggttg caaaattgaa aaatgaggaa tacgcagcaa agtatgttcg
     7081 ggctaagtat caatactcta aagaagggga atttgtctac aatattccag ggttaccaaa
     7141 atgaccgaat ctatttttaa aattgtagag caatttttac agcattctga tgaaaaatta
     7201 gaagagttga atcaaaagaa tagagaatta aagttagaag aaaatgaatc gtagaaagga
     7261 gggagtatgc agaaaaaact gctcgtatgg ttattgccag ttttatttgg atggcaggta
     7321 gtcgatagta cagaaatacc atttgaactc acagcgcaag aagaatacga attgactcat
     7381 actatctatg atcagtattt tcaaactatc cctcaaaacc caaatgtttt tcaaactgag
     7441 aatctttatt cggatgaaga actgactata gcaggtggac aattacagcc gaaccaacat
     7501 ttttctatta cggatgttct agtcaatagc aaaaaagaat tggttttcca aatagatgac
     7561 aagggttaca ttcttgcaag taggcatcta ctatttgatg atgtaattgt tacagaaact
     7621 actgttgagc aaacttattg gacaaaaaaa ggtttcacat tgttaacctc accaattgct
     7681 aacgaagcta cggaaataaa aaatgattta caaccctatc aagctgttat ggtttctaaa
     7741 attgtgacaa cttccctagg tgattttgcc tatgttacag ataaaggttg gattgcagta
     7801 aataatcttt ctaaaacgga taatagagta gaagccgttc aagagttgtt gactaacaaa
     7861 tattccaaag atactattgg tatatatgtg aaacaattgt caacaggtca aactgcaggt
     7921 gtaaatcaag agaaactatt ctattctgcc agcattgcta aactaccaat tctttattac
     7981 gttcaagagc aactgaatgc tgggtatatt gatttgacta ctaaagtgaa atatacagcc
     8041 gaatctatat ccttcccagg agcttatgtt gctggaggaa gtggttcact gtcaaaaact
     8101 cctgataaca aagattattc agtggaagaa ttgatcaata agactgctaa agagtcagat
     8161 aatgtagcta gtaatttact ttcttattat gtagctaata aattcgattc caatttttat
     8221 caagtaataa ctgctaaaac aggtagtgag tggagtatgg ttacacgtga aacttcagct
     8281 gaaatcgcag ggaaaatgat ggaatcctta tatattcaaa atggctatgt gctagaaagt
     8341 ttgttatcta ctcaatttga taatcaacgt atttcgaaag acatctcagt tccagttgct
     8401 cataaaattg gcgatgcaga tgatgtcaaa cacgatgtag cgattgttta cgcagggtct
     8461 ccttttgttt tatctatttt tacagataaa tcaaattatg atgagattac acaaattg
//
LOCUS       DBNFEG010000088         8476 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.088, whole genome shotgun sequence.
ACCESSION   DBNFEG010000088 DBNFEG010000000
VERSION     DBNFEG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.088"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            68..1348
                     /gene="aroA"
                     /locus_tag="AC1PNG_001311"
     CDS             68..1348
                     /gene="aroA"
                     /locus_tag="AC1PNG_001311"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIP6701816.1"
                     /translation="MKLRGSVEKLKGTIRVPGDKSISHRSIIFGSLAKGVTRFHDILR
                     GEDVLSTMQVFRDLGVKIEDNGDIVEVHGVGFDGLQAPKNDLDMGNSGTSIRLISGVL
                     AGQDFEATMFGDDSLSKRPMDRVTIPLSQMGVEISGQTERDLPPLTIKGNKNLKPIRY
                     QLPVASAQVKSALIFAALQAEGESVIVEKELTRNHTEDMIVQFGGQLEVNGKEIRIQG
                     GQEFIAQEITVPGDISSAAFWLVAGLIIPGSKIVLENVGINETRTGILDVIKAMGGKM
                     TFSNIDELAKSATITVETSELKATEIAGELIPRLIDELPIITLLATQAHGTTIIRDAE
                     ELKVKETDRIQVVADALNSMGATIEPTEDGMIIHGPTALHGAEINTFGDHRIGMMTAI
                     AALLAKDGEVVLERAEAINTSYPAFFEHLNSLMD"
     gene            1357..1848
                     /locus_tag="AC1PNG_001312"
     CDS             1357..1848
                     /locus_tag="AC1PNG_001312"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIP6701817.1"
                     /translation="MPIVLLGFMGVGKTTTAHLLNLPVYDMDHIIEERIGMPIADYFS
                     LEGEASFRQLETEVLKELLDLPSNCIVSTGGGVIKSEVNRELLLANREDNVLLTASFE
                     VSYQRIRKDRQSQRPLFLQCSKEEFEALYRERMALYQGLADTVIDTDKLIPEQVARKI
                     LCK"
     gene            1839..2666
                     /gene="pheA"
                     /locus_tag="AC1PNG_001313"
     CDS             1839..2666
                     /gene="pheA"
                     /locus_tag="AC1PNG_001313"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIP6701818.1"
                     /translation="MQVAYLGPRGSFTHQVAQQVFPTADLQAFETITEVIKAYETGEV
                     TYSVIPVENSIEGSVHETIDYLFHQAEIHAVAEIVQPIAQQLLATSADKAVEVIYSHP
                     QAIAQGKKYIQEHFPQARIEITVSTAYAARFVAEHPEQPFAAIAPQAAAVEYGLEVIA
                     QDIQEISENFTRFWILGDKAPSIALEQVAKKVSLALTLPDNLPGALYKAMSVFSWRGI
                     SLTKIESRPLKTALGEYFFILDLDNQSEELLTYSQKELTSLDITYKVLGNYLVYMTS"
     gene            2678..4003
                     /locus_tag="AC1PNG_001314"
     CDS             2678..4003
                     /locus_tag="AC1PNG_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIP6701819.1"
                     /translation="MNKNNNILTHHEQLRLDYLQKNIHYLNDKESRELDYLLYKKELR
                     ERHGSPRQSLRSFTPTAYEDDYFEEDYFEEDDEQEELLPRYPERKSRKTRQAKPQRFQ
                     SPRIGPKAKVPKPKKARKKGGFKRFIGFVLLAIALVIVAMGYSFFKGMNSVSEKPVAE
                     VFNGVDTSNGTNILILGTDGRVGESSGETRTDTIMVLNVNNTDNKVKLVSFMRDTLVD
                     IEGYEYKLNTAYTLGEQDNQQGAEEVRKALKNSFDIDIKYYAMVDFATFATTIDTLFP
                     EGVTIDAQFSTINGEVVSSVEVPNDLQLEENAPAYQTIQVGVQQMDGKTLLNYARYRS
                     DDEGDFGRTRRQQEVLSAVMTQAKNPMKLFSGSEAIGKVVAMTPTTVPQSFMWLQGPG
                     LLLDAANGIERVTIPENGDWIDDYDMYGGMALRVDFEKYQQRLAELGLR"
     gene            4070..5428
                     /gene="rlmD"
                     /locus_tag="AC1PNG_001315"
     CDS             4070..5428
                     /gene="rlmD"
                     /locus_tag="AC1PNG_001315"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIP6701820.1"
                     /translation="MLKKNDIVEVEVVDLTHEGQGVAKIDGFVFFVDNALPGEKISMR
                     ILKLKKNIGFGKVEQWQSFSQDRNQDLDFTYLRTGIADLGHLTYPAQLAFKKKQVENS
                     LRKIAGISEIAVADTLGMDNPLAYRNKAAVPVRRVNGQLETGFFRKNSHDLVPIEDFY
                     IQHKEIDALVLATRDLLRKLDLKPYDEKEQTGLVRNIVVRRGHYSGQLMLVLVTTRPK
                     IFRVETLIERLTSQFPNLVSIVQNINDQNTNAIFGQEFRLLHGSETIADTMLGNEFEI
                     SAPSFYQVNTEMAEKLYQTAIDFAELSADDVVIDAYSGIGTIGLSFAKQVKHVYGVEV
                     VERAVLDSQKNAARNGIDNVTYVCDSAESAMQKWVADGVKPTVIFVDPPRKGLTESFI
                     KASTSVKPEKIVYISCNVATMARDIKLYEELGYKLTKVQPADLFPNTHHIEVVGLLEK
                     RN"
     gene            complement(5509..5762)
                     /locus_tag="AC1PNG_001316"
                     /pseudo
     CDS             complement(5509..5762)
                     /locus_tag="AC1PNG_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            5988..7100
                     /gene="glf"
                     /locus_tag="AC1PNG_001317"
     CDS             5988..7100
                     /gene="glf"
                     /locus_tag="AC1PNG_001317"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HIP6701821.1"
                     /translation="MKKYDYLVVGAGLFGAVFAHEAAKKGKKIKVIEKRDHIAGNIYT
                     KEVEGIQVHEYGAHIFHTSEKEIWDYVNQFAEFNSYTNTPVANYKGEIYNLPFNMNTF
                     NKLWGVVTPAEAEAKIAEQRAVLGGKTPENLEEQAISLVGTDIYEKLIKSYTEKQWEK
                     PCTELPAFIIRRLPVRLTYDNNYFNDTYQGIPIGGYTQIVEKMLEHENIDVETNVDFF
                     ANKEDYLATYPKIVFTGMIDEFFDYQLGELEYRSLRFETEVLDMENYQGNAVVNYTDS
                     ETPYTRIIEHKHFEFGTQPKTIITREYSKTWKRGDEPYYPVNNNRNNYLYTVYKKLSE
                     QQENVIFGGRLGHYRYYDMHQVIGAALQCVRNEVGE"
     gene            complement(7199..7744)
                     /locus_tag="AC1PNG_001318"
     CDS             complement(7199..7744)
                     /locus_tag="AC1PNG_001318"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HIP6701822.1"
                     /translation="MANVLFLVGSLRDGSFNHQMAKKAEALLTDKAQVSYIDLAKIPL
                     FNQDIETPILPEIAELRAQVDAADAIWIFSPVYNYAIPGIVKNALDWLSRSLDLSNPK
                     GPSILQDKITTVSAVANSGHDHLFKDFQHLLPFIRTQIAGEFTGSTINPEAWGTGVLE
                     LSDETLTKLEQQVADLLAAIQ"
     gene            complement(7807..8256)
                     /locus_tag="AC1PNG_001319"
     CDS             complement(7807..8256)
                     /locus_tag="AC1PNG_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIP6701823.1"
                     /translation="MRTVEQSLNENKHALHSLVVFRRAANTIAKSELETIKKYGLTVC
                     QFGVMEALYNKGNLRIQDLIDKLLSTSGNMTVVIKNMIRDGYIYKTIDTNDRRASLIA
                     LTPLGRETIEKILPEHYDHVGEIFSVLSSEEQDQLAEILKKFKNQQT"
BASE COUNT         2597 a         1489 c         1910 g         2480 t
ORIGIN      
        1 tcataaaaat cagaaaatat tttataaata tgataaaatg aaagtgatac tagtttgagg
       61 taaggatatg aaattaagag gaagcgtaga aaaactaaag ggaacgattc gtgtcccagg
      121 tgataaatca atcagtcatc gttctattat ttttggttcc cttgcaaaag gtgttacccg
      181 tttccacgat attttacgtg gggaagatgt cttgtcaacc atgcaggtct ttcgtgattt
      241 gggtgtaaaa attgaagaca atggtgatat tgttgaagta cacggtgttg gctttgatgg
      301 tcttcaagcc cccaaaaatg atttggatat gggaaattct ggaacctcga ttcgtttgat
      361 ttcaggcgtt ttagcaggtc aagactttga ggcgactatg tttggtgatg attccttatc
      421 caaacgtcct atggaccgag taactattcc actcagtcaa atgggagtag agatttctgg
      481 acaaactgaa cgtgacctac ctccgttgac catcaaaggg aataagaatt taaaaccaat
      541 tcgctaccaa ctccctgtag cctctgctca agtaaaatca gctctgattt ttgctgcctt
      601 gcaggctgag ggagagtcgg tcatcgtaga aaaagagttg actcgaaacc atacagaaga
      661 tatgattgtc cagtttggtg gacagttaga agtcaatggc aaggaaatcc gcatccaagg
      721 tggtcaggag tttattgccc aagagattac agttccagga gatatttcaa gtgctgcttt
      781 ttggttggtt gctggcttaa tcataccagg ttcaaaaatt gtccttgaaa atgtgggaat
      841 caatgaaact cggactggta ttttagatgt cattaaagct atgggtggta aaatgacttt
      901 ttctaacata gatgaacttg caaaatctgc taccattaca gttgaaacgt cggaattgaa
      961 ggctacggag attgcaggcg aattgattcc tcggttgatt gatgagttgc cgattattac
     1021 cttactagcg actcaagcac atggaacaac gattattcgt gatgctgagg agttgaaagt
     1081 taaagaaacg gatcgtattc aggttgttgc agatgctcta aatagtatgg gagcaactat
     1141 tgaaccaaca gaagatggta tgattattca cggaccaact gctttacatg gtgctgaaat
     1201 caatacattt ggtgaccacc gtatcggtat gatgactgcc attgccgcct tgttggccaa
     1261 agatggagaa gttgtattgg aaagagcaga agctatcaat actagctatc ctgccttctt
     1321 tgaacactta aatagtttga tggattagga gaacgtatgc caatcgtttt acttggattt
     1381 atgggagttg gaaaaacaac aacagcacat ttgttaaatc tccccgttta cgatatggac
     1441 catatcattg aagaacggat tggtatgcct attgctgact atttcagtct tgaaggagag
     1501 gcttcgttta gacaactgga gacagaggta ctgaaagaat tgctggactt acctagtaat
     1561 tgtatcgtat caactggtgg tggtgtgatt aaatctgaag taaacaggga attgctgttg
     1621 gcaaataggg aggacaatgt acttcttact gcctcctttg aagtttccta tcagagaatt
     1681 cgtaaggata gacagtcgca aagacctctc tttttacagt gtagtaagga agagtttgaa
     1741 gccctctatc gtgaacgaat ggcactttat caaggtttag ccgatactgt aatcgataca
     1801 gataaactga ttccagaaca agtagcaagg aaaattttat gcaagtagcc tatctaggac
     1861 ctcgtggttc ttttacacat caagtggccc agcaggtctt tccgactgcg gatttgcagg
     1921 cttttgagac gattacagag gttatcaagg cctacgaaac gggggaagtt acctattccg
     1981 ttattccagt agaaaactcg attgaaggta gtgttcatga aacgattgac tacctctttc
     2041 atcaggcgga gattcatgcg gttgcagaga ttgttcaacc gattgcccaa caactgttag
     2101 ccacgagtgc cgataaggct gtagaggtta tctattctca tccgcaggca attgctcagg
     2161 ggaagaaata tattcaggaa cattttcctc aggctcgtat tgaaataaca gttagtacag
     2221 cctatgctgc tcgctttgtg gcagaacacc ctgaacaacc atttgcggca attgctccgc
     2281 aagcggcagc agttgaatat ggactggaag taattgcgca agacatccag gaaatcagcg
     2341 agaattttac acgcttttgg attttggggg ataaggctcc gtctattgcc ctggaacaag
     2401 tagctaaaaa agtcagtctt gcactgactt tgccagataa cctgccagga gccttgtata
     2461 aggccatgtc tgttttctca tggagaggaa tcagcttaac aaaaatagaa agccgacccc
     2521 tgaaaacggc tttgggtgag tattttttca tactggatct tgacaatcag tctgaggaat
     2581 tattaaccta ttctcaaaaa gaattgacta gtttagatat aacctataaa gtattaggga
     2641 attacctggt ctatatgacc agctaggatg aagacagatg aataaaaaca ataatatcct
     2701 aacccatcat gaacaattga ggttagatta tttgcaaaaa aatatccact acttaaatga
     2761 caaagaaagt cgcgagttgg attatctatt atataaaaaa gagctgagag aacggcatgg
     2821 aagtccaagg caatctctac gtagcttcac accaacggct tatgaagatg actacttcga
     2881 ggaagattat tttgaagagg atgatgagca agaggaactg ttgcctcgct atcctgaacg
     2941 taaatccaga aaaacaagac aagccaaacc tcaacggttt cagtctcctc gtatcggtcc
     3001 caaagcgaaa gtacctaaac ctaaaaaagc tcgtaaaaag ggtggtttca agcgctttat
     3061 tggtttcgta ttgcttgcta tagcgctagt gattgtagcg atgggctata gttttttcaa
     3121 aggtatgaat agcgtgagtg agaagccagt agctgaggtc ttcaacggcg tagatacatc
     3181 gaatgggaca aatattttaa ttttgggaac agacggtcgt gttggtgaaa gctcaggtga
     3241 aactcgaacg gataccatca tggttctaaa tgtgaataac acggataaca aagtcaaact
     3301 tgttagcttt atgcgggata ccttggtgga tattgaggga tatgaatata aactcaatac
     3361 tgcctatact ttgggagaac aggataatca gcaaggggct gaagaagttc ggaaggcgtt
     3421 gaagaatagt tttgacattg atatcaagta ctatgcgatg gtggattttg caacctttgc
     3481 gacaacgatt gatacacttt tccctgaggg cgtaacgatt gatgcacagt tttcaactat
     3541 taatggtgaa gtcgttagct cagtagaagt gccgaatgat ttacagctgg aagaaaatgc
     3601 tcctgcttat caaacgattc aggtcggtgt acagcagatg gatggtaaga ccttgcttaa
     3661 ctacgcgcgc tatcgttcgg atgatgaagg tgattttggt cgtacgagac gtcaacagga
     3721 agtgctatct gctgttatga cacaggctaa aaatccaatg aaactattct ctggctccga
     3781 agctataggt aaggttgtgg ccatgactcc gacaacagta ccgcaaagct ttatgtggct
     3841 acagggacca ggccttctat tggatgcggc aaatggtatc gaacgtgtta caatcccaga
     3901 aaatggagac tggattgacg actatgatat gtatggtggt atggctcttc gtgttgattt
     3961 tgaaaaatac cagcagcgct tggctgagtt aggcctgcgt taagaaatat ggtatactag
     4021 aagtgacttc ggtcactttt ttagtggtcc aaaaatagaa agtagaatta tgttaaagaa
     4081 aaacgatatt gtcgaagtag aagttgtaga tttgacacat gaggggcagg gtgtggcaaa
     4141 aattgatggt tttgtcttct ttgtagacaa tgctcttcca ggcgagaaaa tttccatgcg
     4201 catcttaaaa cttaagaaaa atattggttt tggtaaggtg gagcaatggc agagcttctc
     4261 acaagaccgt aatcaggact tggattttac ctatctaaga acaggtattg ctgacttggg
     4321 gcatctcact tatccagccc aactagcttt taagaaaaaa caagttgaga atagtctacg
     4381 taagattgca ggcatttcag aaatagcagt agcagatacg ttgggaatgg acaatcctct
     4441 tgcttatcgt aataaggcag ctgttccagt tcgtcgtgtg aatgggcagt tagaaacagg
     4501 attctttcga aaaaattcac atgatttagt tccgattgag gatttttaca ttcaacacaa
     4561 ggaaattgat gccttggttc tagcgacacg tgacctcttg agaaaattag acttgaagcc
     4621 ttacgatgag aaagaacaga caggacttgt gagaaatatt gttgttcgtc gtggtcatta
     4681 ttcaggtcag ttgatgctag ttttagttac gactcgtccg aagattttcc gtgttgaaac
     4741 ccttattgag cgtttgacaa gccaatttcc aaatcttgtt tctatcgttc agaatattaa
     4801 tgaccagaat accaatgcta tattcggtca agaattccgt cttttgcatg gcagtgaaac
     4861 tattgcagat accatgttgg gcaatgaatt tgaaatttct gcaccgtctt tctatcaagt
     4921 caacacagaa atggctgaaa aactctatca gaccgccatt gattttgcag aattgtcggc
     4981 agatgatgtc gtgattgatg cctattcagg tattgggaca attggtctct cctttgcaaa
     5041 acaagttaag catgtttacg gtgtagaagt cgttgaaagg gctgtccttg atagtcagaa
     5101 gaatgctgct cgaaatggga ttgataatgt tacttatgtc tgtgatagcg ctgaatccgc
     5161 tatgcaaaaa tgggtggcag atggagtcaa accaactgtg atttttgtcg acccaccacg
     5221 aaaaggtttg acagaaagtt ttatcaaagc aagtacttct gtaaaaccag agaaaatcgt
     5281 ctatatctct tgtaatgtgg caaccatggc gcgtgatatt aaactttatg aggaattggg
     5341 gtacaaattg acgaaggtac agccggctga tttatttcca aatacgcatc acatagaagt
     5401 tgtgggatta ttagaaaaac gcaattagat tgtccagtat gctctcctgt tttttggcaa
     5461 gcagcttcaa agggccccag gacctttgaa ctctcaaata cgcatcatgt tgacagttgc
     5521 cacacctaca ccaaaatcaa atgccaacaa gcgttgagtc gggtaatagc gtaagtagcg
     5581 caaggtcatg ataagctgct cttccatact tagacggcgt gggcgtcctc cttttcggtg
     5641 ttgctcttga taagcgtcag tgagacaatc aagcatcaga tgaaacgtcg cttttttaca
     5701 cctatcaaca atttgaaatt ctctgagttt aattttaaga ctttttcgta tgttgtttcc
     5761 atacctttag tataccgcct ttgagttacc gaacaagtct attgctaaac ttgatgaagg
     5821 ttgtattgtc tgttataata ttggatattg caagctttaa gtgaaaggtt ttagggaaga
     5881 gattatcaca actcattctt tattagttat gagacgatgg tattaatgat agtctagtaa
     5941 aagatttgga gaaatgactt ccttagaaag ttaaatgtaa aaacaatatg aaaaaatacg
     6001 attatctagt tgtcggagcg ggtctgttcg gagcggtctt tgctcatgag gcagctaaaa
     6061 agggtaaaaa aataaaggtt atcgaaaaac gagaccacat tgcgggtaac atctatacaa
     6121 aagaagtaga aggtattcaa gtccatgaat atggcgcaca tattttccat acttctgaaa
     6181 aagaaatttg ggattatgtg aatcagtttg cggagttcaa cagctataca aacacaccag
     6241 tcgccaacta caaaggtgag atttacaatc ttcccttcaa catgaatact ttcaataaac
     6301 tgtggggcgt ggtgacacct gctgaagcag aagcgaagat tgcagagcaa cgagctgtgt
     6361 tgggtggtaa aacgcctgaa aacttggaag agcaagccat ctcacttgtt ggtacagaca
     6421 tctatgaaaa attgatcaag tcctacacag aaaaacaatg ggaaaaacca tgtacggagt
     6481 taccagcctt catcatccgt cgtttgcccg tgcgtttgac ttatgataat aactacttta
     6541 acgatactta tcaaggtatt ccaatcggcg gttacacgca gattgtagaa aaaatgttgg
     6601 aacatgaaaa tatcgatgtg gagacaaatg ttgatttctt tgccaataag gaagactatt
     6661 tagctaccta tccaaagatt gtctttacag gaatgattga cgagttcttt gattaccaac
     6721 ttggtgaatt ggagtaccgt agccttcgat ttgagacaga agtgttggat atggaaaact
     6781 atcaagggaa tgcggttgtc aactatacag atagcgagac accatatact cgaatcatcg
     6841 aacacaaaca cttcgaattt ggtacacaac cgaaaaccat tattacacgt gagtattcga
     6901 agacctggaa acgaggcgat gagccatact atccagtcaa caataatcgc aataactatc
     6961 tatacacagt atataagaaa ttgtcagaac aacaagagaa tgtcatattc ggtgggcgtc
     7021 ttggtcatta tcgttactac gatatgcatc aagtcatcgg agctgcctta cagtgtgtga
     7081 gaaatgaagt tggggaatga ttttgagaag tcatcaaagt agtaattatt cttggcagaa
     7141 gtcggaatgc tcttaaaatg ttaatcaaaa aaaccagaaa ttctggtttt tttgattatt
     7201 attgaattgc tgccaacagg tcagctactt gttgttcgag ttttgttaga gtttcgtcag
     7261 ataactccaa tacacctgtt ccccatgctt ctggattgat ggttgaacct gtaaattcac
     7321 cagcaatctg agtgcgaata aatggaagaa ggtgttggaa gtctttgaat aggtggtcgt
     7381 gaccactgtt tgcgactgca gagacagttg tgattttatc ttgtaggata gatggtcctt
     7441 ttggattgga taaatcgagc gaacgactga gccaatcgag agcatttttt acgatgcctg
     7501 ggattgcata gttatatact ggtgagaaaa tccagatagc atccgcagcg tctacttgtg
     7561 cacgtagctc tgcaatttca ggaaggatag gtgtctcgat gtcttggttg aataggggaa
     7621 tttttgcaag atcaatgtaa gatacttgag ccttatcagt caagagtgct tcagcttttt
     7681 ttgccatttg atggttaaaa gaaccgtcac gtagtgaacc aactaaaaac aatacgtttg
     7741 ccatgatggc ctccttttca taaaaacata aatttagtat atcgtatttg tcttttgttg
     7801 acaatcctag gtctgctgat tcttgaattt tttaagtatc tcggctaatt ggtcttgttc
     7861 ttctgaacta aggacagaaa aaatctctcc aacgtgatca taatgctctg gaaggatttt
     7921 ttcaatggtt tcgcgtccaa gtggagtgag agcaatcaag gaagcacgac ggtcatttgt
     7981 atcgatagtc ttgtagatat aaccatctcg aatcatgttc tttataacga cagtcatgtt
     8041 gccagaagtg cttaataatt tatcaattaa gtcttgaatc cgcagatttc ctttgttgta
     8101 gagtgcctcc atcactccaa actgacaaac tgtcagtccg tattttttga ttgtttctaa
     8161 ttctgatttt gcgatagtat tagctgctct gcgaaagacg actagactgt gaagtgcatg
     8221 cttgttctcg tttaaagatt gttcaacagt tctcatgaga atattttagc aataaatcgt
     8281 tcggttatca agcaaaaaaa gttaaatcct atattcatag gtaaagtact tttatggaaa
     8341 agatagtttt tagttaatca agttcgtttt ttgaggtaat actaactgta ctggaaaaaa
     8401 acgagcaatg agcatttgag aaatagtctt agatggaagt gcaaaactgt gaatgtaggt
     8461 ttttatatcc ttttct
//
LOCUS       DBNFEG010000089         8462 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.089, whole genome shotgun sequence.
ACCESSION   DBNFEG010000089 DBNFEG010000000
VERSION     DBNFEG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.089"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..775
                     /gene="licT"
                     /locus_tag="AC1PNG_001320"
     CDS             <1..775
                     /gene="licT"
                     /locus_tag="AC1PNG_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HIP6701824.1"
                     /translation="LLFGKGIAFGKKVGDAIPPSSIEKSFLLKNSDNMNRFTELFIDV
                     PIELVYVCEKIINLGKITLGNHFDEIIYINLTDHINSSIERHKEGVVVTNPLRWEIAK
                     YYKEEFELGKKALSIIKKDLHVELPVDEAAFIALHFVNANLENIFQESYRITGIIMKI
                     EQIIQDYYSTEFNQDSIDYYRFITHVKLFAHRLVEGNEYNDEDDVDLLELMKKKYPRE
                     YQCGTRVADFIRLEYDYLLSPSELVYLIAHIRRLTKNLS"
     gene            786..2627
                     /locus_tag="AC1PNG_001321"
     CDS             786..2627
                     /locus_tag="AC1PNG_001321"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HIP6701825.1"
                     /translation="MKYKNTALAILEAVGGEKNVLRATHCVTRLRLELKDENIVSDER
                     VKSISGVLGIMKKNGQYQIILGNDVANYYKEFTALGKFDSDSVQQVKKANVLEQVIEY
                     IAGSMTPLIPAMLGGGMIKVLVIVLPMLGLLKADSQSISFLAFFGDAPYHFMPIFLAY
                     SASQKLKVTPALAMSVAGILLHPNFVQMVSSGDPLHFLGAPVTPASYGSSVIPILIMV
                     WLMKYIEAVFNKVTPAITKSFLQPTLVLLVSGFIALVLVGPLGVIVGEGLSQLVEQMH
                     GVAGWLTLAVLGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVAFKSKNSNTKQIAFAAGFSALFAGVTEPALYGVTLKYKKPLYAAMIGGGLAGLFV
                     GLTGVKAYLFAVPSLIALPQFIYSEAASNITNAMIAAAISIIVTFILAYFLGIDEETS
                     TVNLEKVAPGISSRKNVFSPLSGQILPLEKVNDATFSKKMLGEGVAIIPKDGKVYAPF
                     DGAITSLFPTKHAIGLTSDEGVELLIHFGLETVELKGRGFVSHVSDGEKVEKGQLMLE
                     VDVEMLVAEGYDIVTPVVVTNTQEYLDVLLLSTKEEVNYADDLLAVL"
     gene            2642..4066
                     /locus_tag="AC1PNG_001322"
     CDS             2642..4066
                     /locus_tag="AC1PNG_001322"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIP6701826.1"
                     /translation="MSIFPDNFLWGGAVAANQVEGAYNEGGKGLSVQDVLPNGGLGEA
                     TQEPTEDNLKLQAIDFYHRYKEDIALFSELGFKVFRTSIAWSRIFPNGDEEFPNEEGL
                     QFYDELFDELLKNGIEPLVTLSHYETPLYLARKYHGWVDRKMIQFFEKFARTVFERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDLPNEELSKQDLYQAIHHELVASSLVTKIAHEI
                     IPDCKVGCMVLAMPTYPMTPNPEDVWAAHAYENLNYLFSDIHVRGYYPNYAKRYFKEN
                     GIKITFMEGDEELLKNNTVDFLSFSYYMSITQAADPSKYSSGRGNILGGLVNPYLEAS
                     DWGWQIDPIGLRLTLNRYYDRYQIPLFIVENGLGAKDQLVKDEAGGLTVHDDYRIQYM
                     QEHLLQVAEAIQDGVEVMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGSGSLTRY
                     RKKSFYWYKEVIQSNGSILGNSSK"
     gene            4823..5824
                     /gene="trpX"
                     /locus_tag="AC1PNG_001323"
     CDS             4823..5824
                     /gene="trpX"
                     /locus_tag="AC1PNG_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HIP6701827.1"
                     /translation="MLKNKNLLATIVALTVMVVAALFLTQKEQNNSTNSTEKVKIGVL
                     QFVTHDSLDEIYKGIKAGLEEGGYTTTDNLEIDFMNAEGDQSQVQTMSKKLVDNGNEL
                     LIGIATPAAQSLANATTELPIIMGAVTDPVGANLVTDLKNPGGNITGVSDQTPVADAV
                     SLIKEITPDAKTIGVLYSSNEDNSKIQVAEFKAAAEEAGYTVLEYAVASSNEIAATVE
                     VASSKADVLFTPVDNTVASAFSTVVSVANKTKTPIFTSVEDMVEGGGIASVTLSQYDL
                     GVATGKMAAKILDGANPADTPVQIFNEGTVVVNQKVAKELGITLSDDVISKASKVIE"
     gene            5847..6713
                     /locus_tag="AC1PNG_001324"
     CDS             5847..6713
                     /locus_tag="AC1PNG_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002902146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6701828.1"
                     /translation="MIVSTVSQALLWAVLGLGIFVTFRILNFPDMTTEGSFPLGGAVA
                     VTLLTQGVHPILATLAAVLVGCLAGFVTGLLYTKGKIPTLLAGILVMSSCHSVILFIM
                     GRANLGLLNTKRLQDFLPFSEQVNGLLLGLGSLAVVIGALMFFLYTRFGQAFIATGDN
                     PDMARSFGINTSRMELLGLVLSNGIIALAGALIAQQEGYADVSRGIGVIVVGMASLII
                     GEVIYGNLTMLERFLAIVVGAIFYQFLILVVISLGINTSYLRIFSAMILATCLMVPEL
                     KNKLLKGASLSR"
     gene            6710..7468
                     /locus_tag="AC1PNG_001325"
     CDS             6710..7468
                     /locus_tag="AC1PNG_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6701829.1"
                     /translation="MKAIVELKNATKVVTNGFDEERVILDNVNLTIYQGDFITILGGN
                     GAGKSTLFNVIAGTLPLTSGSVHILGEDVTNWPAEKRAKYLARVFQDPKMGTAPRMTV
                     AENLLIAKYRGEGRGLVPRRLAQQTEEFAQLLPRVGNGLEKHLDTPAGLLSGGQRQAL
                     SLLMASLKQPELLLLDEHTAALDPKTSQSLMQLTDELIETKGMTALMVTHQMEDALRH
                     GNRLIVMKNGQIIQDQTAEEKSKMKLEDYYKLFD"
     gene            7651..8136
                     /locus_tag="AC1PNG_001326"
     CDS             7651..8136
                     /locus_tag="AC1PNG_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HIP6701830.1"
                     /translation="MDKLVFESLADLGMDYIVVEHPPALTTEEADRYIEGLEGVRTKS
                     MFLTNKKKTAFYLLIMDDQKQLDMDRFRDLVGANRIRMASSDSLMEKMHLPAGAVSVF
                     GLLHNVDKDIQVFFDKEILSEPILTFHPNVNTKTIFVKTEDVLRFVEEIGFSAKIVDL
                     G"
     gene            8142..>8462
                     /locus_tag="AC1PNG_001327"
     CDS             8142..>8462
                     /locus_tag="AC1PNG_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701831.1"
                     /translation="MRKCLAICSLLCCLFLVGCRTGAAYQAEIEPMEQSELYTEKEIE
                     AAILVVQDYFDHHFNSCKLLTIGYSGDNEKEFDEWAEHYGAEEAIILTSSFKVAAEGA
                     EPTLE"
BASE COUNT         2505 a         1442 c         1906 g         2609 t
ORIGIN      
        1 attgttgttt ggtaaaggaa ttgcatttgg aaagaaggtc ggagatgcaa tcccaccttc
       61 ttcgatagaa aaaagttttt tgctcaagaa tagcgataat atgaatcgct ttacggaatt
      121 atttatcgat gttcctattg agttggttta tgtttgtgag aaaataataa atttaggaaa
      181 gataacactt ggaaatcatt ttgatgagat tatttacatc aatttgacag atcatatcaa
      241 ttctagtata gaaagacata aggaaggagt agtcgttacg aaccctctta gatgggaaat
      301 tgccaaatat tataaagagg agtttgaact ggggaaaaaa gcattatcca tcataaaaaa
      361 agatttacat gtcgaactcc cagttgatga agcagccttc atcgctttgc attttgtcaa
      421 tgcgaatttg gaaaatattt ttcaagaatc ttatcgtatt actggaatta tcatgaagat
      481 tgaacaaatt attcaagact attattctac agaattcaat caggattcta ttgattacta
      541 ccgattcatc acacatgtta aattgtttgc tcatcgtttg gtagagggga atgaatacaa
      601 tgatgaagat gatgtggatc ttttagagtt aatgaaaaag aagtatccaa gagagtacca
      661 atgtggaact cgagtagcgg actttattcg gttagaatat gattatctac tgagtccaag
      721 tgaattagtc tatttaattg ctcacattcg acgtttaaca aaaaatttat cataaggagt
      781 ctattatgaa gtacaagaat actgcattgg caatcctaga ggcagtcgga ggagaaaaga
      841 atgttcttcg tgcaacccat tgcgttacta gattgcgact ggaattgaaa gatgagaata
      901 tcgtgtccga tgaacgtgtt aaatccattt ctggagtact cggtattatg aagaaaaatg
      961 gacagtatca aattatctta ggtaatgatg tagctaatta ctataaagaa tttactgcat
     1021 taggaaaatt tgattctgat tcagttcaac aggttaaaaa agcaaatgtc ttagaacaag
     1081 tcatcgagta tatcgctggc tctatgacac cacttattcc agctatgctt ggtggaggta
     1141 tgataaaagt tctagtcatt gtcttaccga tgctggggct tttaaaagcg gattcccaat
     1201 ctatttcatt tttagccttt tttggagatg caccatatca ctttatgccg atttttttgg
     1261 cctactcagc ttcacaaaaa cttaaagtta cacctgcttt agccatgtcg gttgctggta
     1321 ttttgctaca tcctaatttt gttcaaatgg tatcttcagg tgatccactt cattttttag
     1381 gtgcccctgt tacacctgct agctatggtt catcagttat tccaattctt attatggttt
     1441 ggctaatgaa gtatattgaa gctgttttca ataaggtcac tccagcaata acaaaaagtt
     1501 tcttacagcc aaccttggtc ctgcttgttt caggtttcat agctttagtt cttgttggac
     1561 ctctcggcgt tattgttggt gaaggattat cgcaattggt tgaacaaatg catggtgtag
     1621 ctggttggtt aactttagca gttcttggag caatcatgcc atttatcgtt atgaccggta
     1681 tgcactgggc ttttgcaccg attttcttgg cagcttcaat cgcgacacct gatgtactga
     1741 ttctaccagc gatgttaggt tctaacttgg cacaaggtgc cgcttctatg gcagttgcat
     1801 ttaagagtaa aaatagtaac acgaaacaaa tagcctttgc agctggattt tcagctttat
     1861 ttgctggtgt cactgagcct gctttatatg gtgttactct aaagtataaa aaaccattgt
     1921 atgcagcaat gattggtggt ggtctagcag gattatttgt tggtttgaca ggtgtaaaag
     1981 catacctttt tgcagtacct tctctcattg ctttgccaca atttatttac tctgaagcag
     2041 cttcaaacat tactaacgct atgattgctg cggcaatttc aattattgtc acatttattt
     2101 tggcctattt cctaggtatc gatgaagaaa cgtcaacagt taatttagaa aaagtagcac
     2161 ctggcatttc aagtagaaaa aatgtcttct ctcctctttc aggtcaaatt ctccctttag
     2221 aaaaagtaaa tgatgcaacg ttttcaaaaa aaatgcttgg agaaggagtt gcaattatcc
     2281 ctaaggatgg aaaagtctat gctccatttg acggggctat taccagtctt tttccaacaa
     2341 aacatgcaat cggtttaaca agtgatgaag gggtagagtt gcttatccac tttggattag
     2401 aaactgtcga gctaaaagga agaggttttg taagccatgt ttcagatggg gaaaaagttg
     2461 aaaagggtca actgatgctg gaagtggatg tagaaatgct tgtcgcagaa gggtacgata
     2521 tagtgactcc tgtagttgtt acaaatacac aggaatatct ggatgttctc ttgctttcga
     2581 caaaagagga agtcaactat gctgatgatt tattagcagt cttataatta gaggagggta
     2641 tatgtcaatt tttccagaca atttcttatg gggaggagca gtagctgcaa accaagtaga
     2701 aggtgcatat aatgagggag gaaaaggatt atccgttcaa gatgttcttc ccaatggtgg
     2761 actgggtgaa gctacccaag aaccaacgga agataatttg aaattacaag ctattgattt
     2821 ttaccatcga tacaaggaag atatagccct gttttcagag ttgggattta aggtatttag
     2881 aacatcaatt gcttggagta gaatatttcc gaatggagat gaagaatttc caaatgaaga
     2941 agggctacag ttttatgatg aactttttga tgaacttcta aagaatggta tagagcctct
     3001 tgtaactctt tctcactatg aaactcccct gtatctggca aggaaatatc atggctgggt
     3061 ggatagaaaa atgattcaat tttttgaaaa atttgctcgt acagtttttg agcgatacaa
     3121 agataaggtt aagtactggt tgacatttaa cgaggtcaat tctgtcttag aactgccttt
     3181 tacaagtggt ggaattgact tgccgaatga agaactttca aaacaagatt tatatcaggc
     3241 cattcaccat gaacttgtag cttctagtct tgttaccaaa attgctcatg aaattattcc
     3301 tgactgtaag gttggctgta tggtcttagc tatgccgacc tatccaatga cacctaatcc
     3361 tgaagatgtt tgggcggctc atgcctatga aaatttgaac tatttatttt cggatataca
     3421 tgttagagga tattatccta attatgccaa aaggtacttt aaagaaaatg gcataaagat
     3481 tacttttatg gaaggtgatg aagagttatt aaaaaataat acggttgatt ttctctcctt
     3541 cagttattat atgagtatta ctcaggcagc agatccatca aaatattcat ctggaagagg
     3601 aaatatcttg ggtggtttgg tcaatcctta tttagaagct tctgattggg gttggcagat
     3661 tgatccaatt ggtttaagat tgacactaaa tcgttattat gatcgatacc agattccttt
     3721 attcattgtt gaaaatggtt tgggagctaa agatcagcta gtgaaggatg aggctggtgg
     3781 tttgactgtt catgacgatt atcgaattca atacatgcaa gaacaccttt tacaagttgc
     3841 ggaggcgata caggatggcg tagaagtcat gggttatact tcttggggct gtattgattg
     3901 tgtatctatg tctaccgcac aattatctaa aagatatgga ctaatttatg ttgatagaaa
     3961 tgatgatgga agcggcagtc taactagata tagaaagaaa tcattttact ggtataaaga
     4021 agttattcaa tccaatggca gtatcttggg caatagctca aaatagactc ttaaggtatt
     4081 aaaaagtttt cagaaaattt aatcaaaagt attgacaaag tgattttgat accgtataat
     4141 catctgtaat cagaagaagt agtaaagcag aagtcttcag ggagcctgtg gtgctgagaa
     4201 caggtggcag agttttatga aatgggctga tgtgcattta tgtctatttt atgacgtcgt
     4261 tagtcggtct tgccgtacca ccagtactgc cttcgcccga ctgcctagtc ctaaaatgcc
     4321 ctaaattcac aaggattgtt tggcataaca tactttgcgt agactgtttt ggctgagtac
     4381 gaggcaggtc gacgcagctt atactagcgg tatagcaagg agagctaacg aagtaatcag
     4441 ccaaaacagt cacgtaaatg aacttaaaac aatgatagtt cggtccgcac accttacagt
     4501 gcaactcctt gttagaggag atgagatatg ggagggttct agacaattcc cataagtgag
     4561 gtggcaccgt gtcattgacg ccctcgcagg aagttttttc ctgcgggggc tttttccttg
     4621 tctttgtaga ttgcttttat attttgttac gattggcttg cctaacttga gttatgtctg
     4681 cgcctccgtt ccttatctaa aagcaatcta ctttgacaat tattaacaaa acgaggtaat
     4741 catgatgaaa cgatggcgat ggtgggataa tatattgatg acgtagaaat tgtatattgt
     4801 gtattttacg aggagaatga atatgttgaa aaataaaaat ttacttgcta caattgttgc
     4861 ccttactgtt atggtagttg ctgccctgtt tttgacgcaa aaagaacaga acaattcaac
     4921 gaatagcaca gaaaaagtca aaattggggt cctccaattt gtgacgcatg attcactaga
     4981 cgaaatttac aagggaatca aagcaggact tgaagaaggt ggttatacca caacggataa
     5041 tttagaaatt gattttatga atgcagaagg tgatcagtca caggttcaaa ccatgagtaa
     5101 aaaattggtg gacaatggca atgaattatt gattggtatc gcaacacctg ctgcacaaag
     5161 tcttgccaat gcaaccacag aattacccat tatcatgggg gctgtaacag atccagtcgg
     5221 agccaacctg gtgacggatt tgaaaaatcc tggtggaaat attacagggg tatctgacca
     5281 aacaccagta gcagacgcag tttctctcat taaagaaatt acaccagacg ctaaaactat
     5341 cggtgtcctc tattcttcaa atgaggacaa ttcaaaaatt caagtagcgg aatttaaggc
     5401 agcagcagaa gaggctggct atactgttct tgaatatgca gttgcttcta gtaatgaaat
     5461 tgcagcgaca gtagaggtcg caagtagtaa agcagatgtt ctctttacac cagtagacaa
     5521 cacagttgcc tcagcatttt caactgtcgt atctgtggca aataaaacca aaactccgat
     5581 tttcactagc gtagaagaca tggtagaagg tggaggtatc gcatctgtta cccttagcca
     5641 atatgacttg ggtgtggcaa ctggtaagat ggctgcgaaa attcttgatg gtgccaatcc
     5701 agcggataca cctgtacaaa tctttaacga gggtaccgtt gttgtcaacc aaaaagttgc
     5761 taaagaatta ggaattacct tgtcagatga tgtgattagt aaagcaagca aggttattga
     5821 ataagagata agagaaaagg aaaaatatga ttgtatcaac agtttcacag gctcttttat
     5881 gggctgtact aggtttggga atattcgtta cctttcggat tctgaatttt cctgatatga
     5941 cgacagaggg gtctttcccg cttggaggag cggttgctgt taccttattg acgcaaggag
     6001 ttcatcctat cttggctact ctagcagcag ttcttgttgg ctgtttggcg ggctttgtca
     6061 caggcttgct ttataccaag gggaaaattc ccactctcct agctggtatc ttggtcatgt
     6121 cttcctgtca ctctgtcatt ctatttatca tgggtagggc aaatctaggc ttactgaata
     6181 ccaaacgttt acaagatttc cttccttttt ctgagcaagt caatggtctc ttgctaggtc
     6241 taggtagcct agcggtggtg attggtgcct tgatgttctt cctctatact cgctttggac
     6301 aggcctttat tgcaaccggg gataatccag atatggctcg cagttttggt atcaatacaa
     6361 gtcgaatgga gttgctaggt cttgttcttt caaatgggat catcgctctt gcaggtgcct
     6421 tgattgcaca acaagaaggc tatgcggatg tgtcgcgtgg tattggtgtc attgtggtcg
     6481 gtatggctag tctgattatc ggtgaggtaa tttatggtaa ccttactatg ttagaacgat
     6541 tcttggccat tgttgtcgga gcgattttct atcagttctt aattttggtg gtcatttccc
     6601 tcggtatcaa taccagctac ctccgtatct tctcggccat gattctggca acttgcctca
     6661 tggttcctga gttgaaaaat aaacttctga aaggagccag tctgtcacga tgaaagcaat
     6721 tgttgaatta aaaaatgcca ccaaggtggt gacaaacggt tttgatgaag aacgtgtgat
     6781 tttggataat gtcaatctga ccatttacca gggtgatttt attaccattt tgggtggaaa
     6841 tggggctggg aagtcaaccc tctttaatgt cattgctgga accctaccct tgaccagtgg
     6901 ttcagtccat attcttggtg aggatgtgac caattggcct gcagaaaaac gtgctaagta
     6961 tctggcacgt gttttccaag atcccaaaat gggtacagcg ccacggatga cggtggcaga
     7021 aaatctactg atagctaagt accgtggcga ggggcggggc ttagtgccac gcagattggc
     7081 ccaacagacg gaagaatttg cccagctttt gccgagagtg ggaaatggtt tggaaaaaca
     7141 tttggacacg ccagcagggc tcttatcagg tggtcaacgc caggcattga gtttgttgat
     7201 ggcgagtttg aaacagcctg aacttttact cttggatgag catactgcag ccctcgaccc
     7261 taaaaccagc caatccttga tgcaattaac ggacgagttg attgagacca agggaatgac
     7321 ggccctcatg gtcactcacc agatggaaga tgctctgcgt cacggtaatc gcttgattgt
     7381 catgaaaaat ggtcagatta ttcaggacca aacggctgaa gaaaaatcca agatgaaact
     7441 ggaagattat tataagttgt ttgattaaga acctgtgtgg atagttcgca acttccaacc
     7501 aagtttggtt ttggctaaaa ctgaatctta tatcaaacag aacacttgtg aatacaggtc
     7561 taaagtctgg agtaatccag gcttttatta tcctttgaat ttttagttaa aaagtagtat
     7621 aatatactaa atatgtcaag aggtgctgtc atggataaat tggtttttga aagcttagct
     7681 gacttgggaa tggactacat cgttgtagag catccaccag ctctgacaac tgaggaagca
     7741 gaccgttaca ttgaagggtt agaaggagta cggaccaagt ccatgtttct gactaataag
     7801 aaaaagactg cattttattt actgatcatg gatgaccaga agcagctgga tatggaccgg
     7861 tttcgtgatt tggtgggagc caaccgcatt cggatggcct cgagtgatag cttgatggaa
     7921 aaaatgcact tgcctgcggg tgcggtatct gtttttggct tgttacataa tgttgataag
     7981 gatattcaag tcttttttga taaagaaatc ctttctgaac ccattctaac cttccacccc
     8041 aatgttaata cgaaaacgat ttttgtaaaa acagaggatg ttttgcgttt tgtagaagag
     8101 attggctttt cagcaaagat tgttgacttg gggtaacaaa gatgagaaaa tgtttagcta
     8161 tttgtagtct gttgtgttgt ctttttttgg taggatgtcg gacaggagca gcttatcagg
     8221 cagagattga accgatggaa caatcagaat tgtatacgga aaaagagatt gaggcggcta
     8281 ttcttgtagt tcaagattac tttgatcacc attttaacag ttgtaaattg ctgaccatcg
     8341 gttacagtgg tgataatgag aaggaatttg atgagtgggc ggagcactat ggtgcagagg
     8401 aagctattat cctaacttcg tcttttaagg tagcagctga gggggcagag cccaccttag
     8461 ag
//
LOCUS       DBNFEG010000090         8416 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.090, whole genome shotgun sequence.
ACCESSION   DBNFEG010000090 DBNFEG010000000
VERSION     DBNFEG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8416
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.090"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            27..874
                     /locus_tag="AC1PNG_001328"
     CDS             join(27..330,330..874)
                     /locus_tag="AC1PNG_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HIP6701832.1"
                     /translation="MAYSLDFRKKVLAYCEKTGSITEASAIFQVSRNTIYQWLKLKEK
                     TGELHHQVKGTKPRKVDRDKLKNYLETHPDAYLTEIASEFDCHPTAIHYALKAMGYTP
                     KKKSCTYYEQDPEKVELFLKELNNLSHLTPVYIDETGFETCFHREYGRSLKGQLIKGK
                     VSGRRYQRISLVAGLINGALIAPMTYKDTMTSGFFEAWFKTFLLPTLGKPSVIIMDNA
                     KFHRMSKLKDLCEEQGHRLLPLPPYSPEYNPIEKIWAHIKKHIRRVLTNCDTFLEVLS
                     SCSCFS"
     gene            complement(878..1666)
                     /locus_tag="AC1PNG_001329"
                     /pseudo
     CDS             complement(878..1666)
                     /locus_tag="AC1PNG_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774941.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
     gene            1792..2325
                     /locus_tag="AC1PNG_001330"
     CDS             1792..2325
                     /locus_tag="AC1PNG_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HIP6701833.1"
                     /translation="MGTDSRKERTRQAILEAMVTCLESQGFNDITTTHLAQTAGISRS
                     SFYTHYKDKYELIDSYQQGLFHKLEAIFDRYDGNRQSSFLEIFELLYREKLLSALLTH
                     NGTQEIQNFLINKVRLLVANDLAERLGKEDLSPLEKEYSSIYFSHAFFGIMQVWIKNG
                     KQESPQYMTDFLIKMLP"
     gene            2410..3393
                     /locus_tag="AC1PNG_001331"
     CDS             2410..3393
                     /locus_tag="AC1PNG_001331"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6701834.1"
                     /translation="MIQAEHLSKTYSIIDKEVGLKGSIKAFFKPKKKSIPAVQDISLQ
                     VGKGEIIGYIGSNGSGKSTTIKMLTGVLYPDQGQVRINGLNPQENRKAVNKQIGVLFG
                     QKSHLDWNLPVQESFILHAKIYDVPDKVFKERLAVLIELLDLADIMKQPIRNLSLGQR
                     VRCEFAAIFIHQPAVVFLDEPTIGLDASVKETIRSFIRYMNQEYQTTFLITSHDMKDI
                     ESLCERIFIIDKGKKVYDGSLTVLKERFSTVKTILFSTEKPIEKDLQLEGWEFIRKDD
                     FHFEIHYQNKVWTSAQVIEQVFNHYAIEDVTMKELEIETMLRQIYEEGIHA"
     gene            3386..4165
                     /locus_tag="AC1PNG_001332"
     CDS             3386..4165
                     /locus_tag="AC1PNG_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6701835.1"
                     /translation="MRKYLYMTRLTMMNAMQYKAFFLATFVSLAVKILVALYVWKTIF
                     FTQSEVNGFTLQTFTTYIIFANLLASLNSFSLGEDLSYSILKGSIAGEFLRPYSFILA
                     LFFKDLGNKLLELIKFAIVFIGILLVHQDFYLPDGKTILLFLVSSILGMFIVQLLDMA
                     FGFLAFFTVNAWGVMLLRMGLFNLASGALLPLSFYPQAVENFLKLLPYNYAVNVPVSI
                     LLGQESDLTALGLQVIWIPILACFIALLWSQAKRRIVIFGG"
     gene            4167..4949
                     /locus_tag="AC1PNG_001333"
     CDS             4167..4949
                     /locus_tag="AC1PNG_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6701836.1"
                     /translation="MKKYISLYLYNIKVYMMAQMSFRVDFFIGLFSSLIEQIVYLIFL
                     NILFGNIKEIAGFNYGQMLFIYGIATVGRSIHLIFFDNLWMFGSRYIRQGEFERLLLM
                     PVNPLFQLICEQIQPQGIGTTLIGSLALVQASKELGMEWSLGKLALLIFIAICIGLLY
                     AAIQLGPTALAFWIVESFPLTMGIFALNQMAQYPLNIYPKIIQILLIFVFPYAFTAYF
                     PALYFLDLSMWGLALPLVVVVLFSINYNLFRYGMTKFTSVGN"
     gene            complement(4999..5826)
                     /locus_tag="AC1PNG_001334"
     CDS             complement(4999..5826)
                     /locus_tag="AC1PNG_001334"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIP6701837.1"
                     /translation="MIVRLIATDMDGTFLDGQGNFDRERFSRVLDQLDQKKIPFVIAS
                     GNGIGRLLQLCQGFEDRLIFVADNGAHVYQNGKTVIRRAIQQVEVEAILHFFKGRWAD
                     VCLMLSNDGNIYMQAGAGMPFAGTDLPIESAQMVAFQNRVTYLDDLSAYPNFEPIYKV
                     GLWVPEARVESITEEFNQAFQGRLTAVTSGYGSIDILPEGIHKAWGLEQVLKDLVIQP
                     DQVMAFGDSDNDIELLSYVGYSYAMENATDKVKAVAKYMAPSHLEAGVLQVIEEYIL"
     gene            complement(5838..7070)
                     /locus_tag="AC1PNG_001335"
     CDS             complement(5838..7070)
                     /locus_tag="AC1PNG_001335"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIP6701838.1"
                     /translation="MERRANYIRRKRYASYRTKMHLFFICGLATIILAIIIVSEHLVP
                     RLNQASESMHSDNSVRIMAHGDLLYHLPILRGAEQPDGSYDFSENFTYVKPWIEQADF
                     AIADFEGTISPDIELAGYPLFNAPAIVANNIHDAGYDLVDLAHNHILDSHLSGLISTV
                     NTFRDAGVDTVGVYAEGNRATAPLYIREVNGIRIAVLAYAYGFNGMEALLSQEEYDAY
                     LSDFDKEKMRAEIERAEKEADITIVMPQTGVEYQLEPTEEQTSLYHQMIDWGADIVLG
                     GHPHVVEPTEILEKDGQRKLIIYSMGNFLSNQRIESMEDTPNAQWTERGVLMDLTIQK
                     ENGQTSIQTAQAHPTWVNRLSKGTYSTDGYEQFSYQTYILEDWVAGGRYYGQLDLDTQ
                     ARVDTVYQEMKDFVNLVW"
     gene            complement(7403..8341)
                     /locus_tag="AC1PNG_001336"
     CDS             complement(7403..8341)
                     /locus_tag="AC1PNG_001336"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HIP6701839.1"
                     /translation="MVSTATTLGGFTFDNCLMNAAGVWCMTKEELDAVKNSKAGTFVT
                     KTATLDYRAGNPEPRYQNVPLGSINSMGLPNQGLAYYLDYLLELQETESERTFVLSVV
                     GMSPDETHEILRTVEASDFKGLTELNLSCPNVPGKPQIAYDFETTETILQEVFTYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNQFPLKFVNCVNSIGNGLYIKDESVVIKPKNGFGGI
                     GGEYIKPTALANVHAFYQRLKPEIQIVGTGGVLTGRDAFEHILCGASVVQVGTTLQKE
                     GVAAFGRITAELQAIMAEKGYETIEDFRGKLQHLDD"
BASE COUNT         2429 a         1916 c         1543 g         2528 t
ORIGIN      
        1 cagtacattt atgatataat gtatttatgg catattcatt agattttcgt aaaaaagttc
       61 tcgcatactg tgagaaaacc ggcagtatta ctgaagcatc agctattttc caagtttcac
      121 gtaacactat ctatcaatgg ctaaaattaa aagagaaaac cggcgagctt catcaccaag
      181 ttaaaggaac caagccaaga aaagtggata gagataaatt aaagaattat cttgaaactc
      241 atccagatgc ttatttgact gaaatagctt ctgaatttga ctgtcatcca acagctattc
      301 attacgccct caaagctatg ggatatactc gaaaaaaaag agctgtacct actatgaaca
      361 agaccctgaa aaagtagaac tgttccttaa agaattgaat aacttaagcc acttgactcc
      421 tgtttatatt gacgagacag ggtttgagac atgttttcat cgagaatatg gtcgctcttt
      481 gaaaggtcag ttgataaaag gtaaggtctc tggaagaaga taccagcgga tatctttagt
      541 ggcaggtctc ataaatggtg cgcttatagc cccgatgaca tacaaagata ctatgacgag
      601 tggctttttc gaagcttggt tcaaaacatt cttactaccc actttaggta aaccatctgt
      661 tatcatcatg gacaatgcaa agtttcatag gatgagtaag ctaaaagatt tatgcgagga
      721 gcagggacat agacttttac cacttcctcc ttactcaccg gaatataatc ccattgagaa
      781 aatatgggct cacatcaaaa aacacatcag aagagtattg acaaattgcg atacttttct
      841 tgaggtactt tcgtcctgct cttgtttcag ttgactataa ttgctccgca cctgaagcaa
      901 gacttgctac tccatttgtg taggtcgtta agccactgga cagtgtttga ctgcctatcg
      961 cagcctgatt gactccatct gtataagaca caactccggt ggccaattgg tttgctcctg
     1021 tcacgggtga ttgacccgaa gtcgttaaag tagtcaatcc atttgaaagt gtttgactgc
     1081 ctgactccaa ttgactagct ccatctgtta attgttgact tccatctgcc gcctcgacca
     1141 tgccggtttg aatttctctc atactatcaa aaacggcagt cgtgtaggtt ttggtaattg
     1201 tctctgaaac agatgtcttt aatttctcca tagctgattc gcccatctta gaagcaacaa
     1261 agctatgacc tttggaggtt tgataagaaa tcgttatcgg ttcaggctga ttcgtcaaga
     1321 gactggttgc tttttctgat aaatcttctg gtagagtgat gaccatataa tagtcccctt
     1381 cttccaagcc tttctccgca tccttttctg atacaaagtg gaaatccaga cttttgctct
     1441 ctttcatatt atccaccata tcatcaccaa tggtaagggt tttatcttga aagctcgccg
     1501 actggtcttg attgatcact gctacaggaa gctggtctag tcggccgtaa gggtcccaca
     1561 tagaggttag gaaactaatg ttatataagg ctgggacaca ggcaatacca ataatggtaa
     1621 ccaaagtcat tggttttttg aaaatattct tcacttttcg taacataagc aactccttta
     1681 gacatactgt ccaaaaactg ataaaataca ttatacaagt atcattttca aaatcaagtc
     1741 ataagaaccg tttttagaca ctgtgtctaa aaatgttcaa aaggagaaat tatgggaaca
     1801 gatagtcgga aagaacgaac tcgacaggca attttagaag ctatggtaac ctgcctggaa
     1861 tcacaaggat ttaatgacat tacaacaaca cacctagccc agactgctgg tatcagtcgt
     1921 tccagttttt atacccacta taaggataaa tatgaattaa ttgactccta tcaacaggga
     1981 ctttttcaca aattagaggc tatatttgac cgttatgatg gcaatcgcca atcatcattt
     2041 ttagaaattt tcgagttact ctatcgtgaa aaattgcttt ctgccctact gacccataac
     2101 ggaactcaag aaatccaaaa ttttcttatc aataaagtcc gacttcttgt tgccaatgat
     2161 ctagcggagc gcttggggaa agaagattta tcaccacttg aaaaagaata cagcagcatc
     2221 tatttttcac atgccttctt cggtatcatg caggtatgga ttaaaaatgg taagcaagaa
     2281 tctcctcaat atatgaccga ttttctaata aaaatgttac cttaaaggtg actttttacc
     2341 gccactttct gggaaatatg ttataatatc cttatcttac gcaaacgttt tcaaacagga
     2401 gattatgata tgattcaggc agaacatctt tcaaaaacct attcaatcat tgataaggaa
     2461 gtcggtctaa aaggctctat caaggcattt ttcaagccaa agaaaaagtc aatccctgct
     2521 gttcaagata tttccttaca ggtaggaaaa ggagaaatca tcggctatat cggttccaac
     2581 ggttctggta aatccaccac catcaaaatg ctgacaggtg ttctctatcc agaccaagga
     2641 caggttcgta tcaacggact caatccgcaa gaaaaccgca aagctgtcaa taagcaaatc
     2701 ggcgttctct ttggacaaaa atctcatttg gattggaact tgcctgtcca ggaatctttt
     2761 atccttcacg caaaaattta tgatgttcca gataaggtat tcaaagagcg attggctgtc
     2821 ttgattgagc tattggactt ggctgatatt atgaagcaac caatccgtaa tctgtcactc
     2881 ggccaacggg ttcgctgtga atttgcggct atctttatcc accagcctgc cgttgttttt
     2941 ttggacgaac caaccatcgg cttggatgcc agcgtcaagg aaaccattcg ctccttcatc
     3001 cgctatatga atcaagaata ccagaccact tttttgatta cttcccatga tatgaaggac
     3061 atcgaaagcc tctgtgagcg gatttttatc attgataagg gcaagaaggt ctacgatggt
     3121 tcactgaccg tcctcaaaga acgcttttcc acagtcaaga ccattctctt ctccacagaa
     3181 aaaccaattg aaaaagattt gcaattggag ggctgggaat ttattcgtaa ggatgatttt
     3241 cactttgaaa tccactacca aaacaaggtc tggaccagcg cccaggtgat tgagcaagtt
     3301 ttcaatcatt atgccattga agatgttacc atgaaggaat tggaaattga aaccatgctc
     3361 cgtcagattt atgaggaggg tatccatgcg taaatatctc tacatgaccc gcctgaccat
     3421 gatgaatgct atgcagtata aggctttctt tctagcgact ttcgtttcac tagcagtgaa
     3481 gattttggta gctctctatg tctggaaaac cattttcttc actcaatcag aagtcaatgg
     3541 ctttacccta caaaccttta ctacatatat catctttgct aatctactag ccagtttaaa
     3601 ttctttctca ctcggagaag atttatccta cagcatttta aaaggaagca ttgctggaga
     3661 atttcttaga ccctattcct ttatcctagc cctctttttc aaagaccttg gaaataagct
     3721 tttggaactg ataaaatttg ctatcgtatt tatcggtatc ttgcttgttc atcaggattt
     3781 ttatctacct gacggaaaaa ctattttact ctttctagtc agctccatcc tcggtatgtt
     3841 tatcgttcaa ttgctggata tggcttttgg cttcttagca ttttttacgg tcaatgcctg
     3901 gggagtcatg cttctgagaa tggggctttt caatctagct tctggagcgc ttttgccact
     3961 cagcttctat ccacaggctg tggaaaactt tttgaagctc cttccctata attatgcggt
     4021 taacgttcca gtttccattt tattgggaca ggaaagcgat ctaactgctt tgggattgca
     4081 agttatatgg atacccattc tagcctgctt catcgctcta ctctggtccc aggccaagcg
     4141 tcgtattgtc atttttggag gttaacatga agaaatatat cagcctctat ctatataaca
     4201 tcaaggtcta catgatggcc cagatgtcct ttcgagtgga cttcttcatc gggcttttct
     4261 cctctttgat tgaacaaatt gtctacctga ttttcctcaa tattctcttt ggaaatatca
     4321 aggaaatcgc tggttttaac tacggtcaaa tgcttttcat ctatggaata gcgaccgtcg
     4381 gacgctccat tcacttgatt ttctttgaca atctctggat gtttggtagt cgctacatcc
     4441 gacaaggcga atttgaacgc ctcctcctca tgcctgtgaa cccacttttt caacttatct
     4501 gtgaacaaat tcagccacaa ggaatcggaa caaccctcat cggttccctt gcactagtcc
     4561 aagcttccaa ggaactgggt atggaatgga gtcttggaaa actagcctta ctaatcttta
     4621 ttgccatttg tatcgggcta ctctatgcag ccattcaact cggtccaact gctctagcat
     4681 tttggattgt ggaatcattt cctttgacta tgggaatttt cgccctcaat cagatggctc
     4741 aatacccact caacatctat ccaaaaatca ttcaaatcct gctgatattt gttttcccct
     4801 acgcttttac cgcttatttc ccagccctct acttcttaga cttgtctatg tggggactgg
     4861 cgttgcctct ggtggtagtg gtcctatttt ccatcaatta taacctcttc cgctatggta
     4921 tgaccaagtt caccagtgtt ggaaattaaa aaaagctaga attctctgga ttgagagcac
     4981 tagctttttt ctatatactt ataaaatata ttcttcaatg acctgaagca cacctgcttc
     5041 caaatggctt ggagccatgt atttggcaac cgccttgacc ttgtctgtcg cattttccat
     5101 ggcataggaa tacccaacat aggacaataa ctcaatatca ttgtctgaat caccgaaggc
     5161 catgacttga tcaggttgta ttaccagatc cttcaaaact tgttccaagc cccatgcctt
     5221 atgaatccct tctggcaata tatcaatgga gccatagcca cttgtcactg cagtaagacg
     5281 accttgaaaa gcctggttaa actcttccgt tatcgattct acacgcgctt caggaaccca
     5341 gagaccaacc ttataaatag gttcaaaatt aggataggca ctgaggtcat ctaaatatgt
     5401 cacacgattt tggaaggcaa ccatctgagc tgattctatt ggcaaatcag tccctgcaaa
     5461 tggcatacct gccccagcct gcatataaat atttccatcg ttcgacaaca tcagacatac
     5521 atccgcccaa cggcctttga aaaaatgcag tattgcttcg acttctactt gctgaatagc
     5581 ccgacgaatt actgtcttgc cattttgata cacatgggct ccattatccg ctacaaagat
     5641 taatctgtct tcaaatcctt ggcataattg tagtaatcgc ccaattccat taccactagc
     5701 gataacaaaa ggaatctttt tctgatctag ttgatccaag acccgtgaaa atcgctctct
     5761 atcaaaattc ccctgaccat ctaaaaaggt cccatccatg tctgttgcaa ttaatcgaac
     5821 tatcatcttc tttctcctca ccataccaaa ttgacaaagt ctttcatttc ttgatagact
     5881 gtatccacac gcgcctgtgt atctagatcc aattgtccat agtatcgacc accagcgacc
     5941 cagtcttcca aaatgtaagt ctggtaggaa aattgttcgt atccatctgt tgaataagtc
     6001 ccttttgaaa ggcgattgac ccaggttgga tgggcttgag ccgtttgaat acttgtttgc
     6061 ccattctctt tctgaatagt aaggtccatc aagacacctc tttctgtcca ctgggcattt
     6121 ggggtatctt ccatactttc gatacgctga ttggatagaa aattccccat agaatagatg
     6181 attaattttc tctgaccatc tttctcaaga atttccgtcg gctcgactac atgcggatgc
     6241 ccaccaagaa cgatgtctgc tccccaatca atcatttgat ggtacaaact ggtctgttcc
     6301 tccgtaggct ccaactggta ttctacaccc gtctgaggca tgacgatggt aatatctgcc
     6361 tctttctcag ctcgctcaat ctctgcccgc atcttttctt tatcaaaatc cgataaatag
     6421 gcatcatatt cctcctgact cagcaaagcc tccatcccat tgaaaccgta agcataggct
     6481 aggacagcta tacggatacc attgacttcc cgaatgtaga gtggtgcagt tgcccgattg
     6541 ccttcggcgt agacaccgac tgtatccaca cctgcatctc taaatgtatt gacagtggaa
     6601 atcagccctg atagatgtga gtccaagata tgattgtggg ctaagtccac caaatcgtag
     6661 cctgcatcat ggatattgtt ggcgacaatg gcaggcgcat taaaaagtgg gtaacctgct
     6721 aattcgatat ctggagaaat ggtcccttca aagtctgcga tagcgaaatc agcctgttct
     6781 atccagggct ttacgtaggt aaaattctca gaaaaatcgt aacttccatc tggctgttct
     6841 gctcctcgaa gaataggcaa gtgatagagc aaatccccat gagccatgat gcgaacagaa
     6901 ttatctgagt gcattgattc tgaggcctga ttcagcctag gaactaggtg ctcgcttaca
     6961 ataataatcg ccaagataat agtcgctaag ccacaaataa aaaacaaatg catctttgtc
     7021 cgataggacg catatcgttt tctgcgaata tagttcgccc ttctctccat acctttaccc
     7081 tcatactccc tacttaagaa cctttgttca aaatacagtc agcagtccct caaaaaacta
     7141 tttcttccat aaaagcacat cacttataaa tagaggttct aatattagta tacgatattt
     7201 ttgctaaaaa atctaaataa aatcagaatt ccatctcaca aatatatgtt tctgactatg
     7261 tattccattc ctcaaacgat tatcagaccg atcttcacag accatttaaa aagagctaac
     7321 attatcccct cgcgactccc taaaagacta tctgggagaa cagtacaatg ttagcttctt
     7381 tttatgtctt caacagatga tctcagtcat ctaagtgctg tagcttgcca cgaaaatctt
     7441 caatggtctc gtagcctttt tcagccatga tggcctgcaa ttcagccgta atgcgtccaa
     7501 aggctgccac accttccttt tgcagggttg ttccaacttg aaccacgcta gctccacaga
     7561 gaatgtgttc gaaggcatcc cgacctgtca agacgccacc agtcccaaca atctgaatct
     7621 ccggtttcaa gcgttggtaa aaagcgtgga cattggcaag agcagtcggc ttgatatact
     7681 ctccaccgat accaccaaaa ccatttttag gcttgatgac aactgactca tccttgatat
     7741 agagaccatt tccaatcgaa ttgacacagt tgacaaactt caaaggaaac tggttgaaaa
     7801 tcgctgctgc ttggtcaaaa tgcacaatat cgaaataagg tggtaactta ataccgagtg
     7861 gtttggtaaa gtaagtgaag acctcctgca agatggtttc tgtcgtttca aaatcatagg
     7921 caatctgcgg ttttccaggg acattgggac aagaaagatt taactctgtc agccccttaa
     7981 aatcactggc ttctacagtt ctcaaaattt catgagtctc atcaggcgac attccaacta
     8041 cagataaaac aaatgtgcgt tctgactccg tttcctgcaa ttctagtaga taatccaaat
     8101 agtaagccaa gccctgattt ggcaaaccca tcgaattgat agaaccaagc ggaacattct
     8161 gataacgtgg ttctggatta cccgcgcggt agtccaaggt tgctgttttt gttacaaaag
     8221 tacccgcctt cgagttctta accgcatcca attcttcctt ggtcatgcac caaacccctg
     8281 ccgcattcat caaacaatta tcaaaggtaa atccaccgag tgtcgttgct gttgaaacca
     8341 tattatcctc catataggtc attctttctt gattatatca taagaaacaa acggattttc
     8401 cccatttact actaac
//
LOCUS       DBNFEG010000091         8298 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.091, whole genome shotgun sequence.
ACCESSION   DBNFEG010000091 DBNFEG010000000
VERSION     DBNFEG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.091"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            136..2085
                     /gene="parE"
                     /locus_tag="AC1PNG_001337"
     CDS             136..2085
                     /gene="parE"
                     /locus_tag="AC1PNG_001337"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HIP6701840.1"
                     /translation="MAKKEIDINNYNDDAIQVLEGLDAVRKRPGMYIGSTDGNGLHHM
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLSVSDRGRGMPVGMHATGKPTVEVIFTVL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFEQGGKPVTTLE
                     KIGTAPKSKTGTKVTFMPDDTIFSTTDFKFNTIAERLKESAFLLKQVTMTLTDERTGE
                     QEEYHYENGVQDFVSYLNEDKETLTPVLYFEGEDAGFQVQVAMQYNDGYSDNILSFVN
                     NVRTKDGGTHETGLKLAITKAMNDYARKTNLLKEKDKNLEGSDYREGLSAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVSDKLTFFLLENGELASNLVRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGSDFTLEDVNYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGRVYIALPPLYKMSKGKGKTEKIAYAWSDGELED
                     LRKDFGKGFILQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVSVLMGD
                     KVEPRRKWIEDNVKFTLEEATAFTK"
     gene            2109..2711
                     /gene="fic"
                     /locus_tag="AC1PNG_001338"
     CDS             2109..2711
                     /gene="fic"
                     /locus_tag="AC1PNG_001338"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HIP6701841.1"
                     /translation="MVLENKLGIENSAELARLEEQISKKKAAQLFETGQLFQIEVGTF
                     AGLAHIHQTLFEEIYDFSGKIRDVNIAKDNFQFAPRIFLEQSLAYIDKLPHETFDEIV
                     EKYADMNIAHPFREGNGRSMRIWLDCMLRDSLGKVVDWNSIDKDEYFNAMVRSHVSTG
                     ELKYLLLQALTEDLGQATYFKGIDHSYYYEGYNLYRTEDL"
     gene            2744..3238
                     /locus_tag="AC1PNG_001339"
     CDS             2744..3238
                     /locus_tag="AC1PNG_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HIP6701842.1"
                     /translation="MPKLTDRFQKRLSEWARKRKPFKNKQELEEKFDQALKIWQSSKT
                     RQHAENLLSEQGKLEHFLHSTERKLSRMPFGGDKFAAIPGLISMVRSYIRRDYSKLPK
                     GTILAIIGALIYFFSPIDALPDFILGAGLLDDAFVLNACLKLIKTDVDDFRSWQASQW
                     KVDE"
     gene            3241..3693
                     /locus_tag="AC1PNG_001340"
     CDS             3241..3693
                     /locus_tag="AC1PNG_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701843.1"
                     /translation="MIKFLRKKLTIEQLKKVPYASQYTEVLRSIWRADVPKYGISSTL
                     QGELLRQLEKLRWEAQANGNVNWCEEHSNYCRFIKETLYKGKVLSSQQKQELVLIMDY
                     LKSCGEYAQAYQENLIDDEELEIEKLAYVDDNLYDRVGDMIAFFYQRT"
     gene            3894..6347
                     /gene="parC"
                     /locus_tag="AC1PNG_001341"
     CDS             3894..6347
                     /gene="parC"
                     /locus_tag="AC1PNG_001341"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HIP6701844.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFDKGYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQDWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEMAGYLLADIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGATGISAGYATDIPPHNLAEVIDAVVYMIDHPTAKLEKLMEFLPGPDFPTGAI
                     IQGADEIKKAYETGKGRVVVRSRCEIEQLKAGKKQIVITEIPYEVNKAVLVKKIDDVR
                     VNNKVPGIAEVRDESDRTGLRIAIELKKDSDEQTILNYLYKYTDLQINYNFNMVAIDN
                     FTPRQVGLQKILSSYIAHRREIIIARSKFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFSEEQAEAIVTLQLYRLTNTDIVTLENEEAALREQIQTLA
                     AIIGDERTMFNLMKKELREVKKQFGNPRLSELQDQAETIEIDTASLIVEEETFVSVTK
                     AGYIKRTSPRSFNASTLEEMGKRDDDQLIFLQNAKTTQHLLLFTNLGNVIYRPVHELT
                     DIRWKDIGEHLSQTLMNFDTSEEIIFAELVENFEEGTYFAVTQYGQIKRVERKEFTPW
                     RTYKSKSTKYAKLKDAEDVVITVSPVVLDDIMLMTEKGYALRFNIEEVPIIGAKAAGV
                     KAVNLKDGDVVVAAFISNTSSVYLLTQRGSFKRMATEEIPVTSRAKRGLQVLRELKAK
                     PHRVFAAGPVLTDQGDFDLFSTANEDETTSQILHVQSKTGKLYEVDVTQLSLSERTSN
                     GSFISDTISDEEVFRAWID"
     gene            6519..7136
                     /locus_tag="AC1PNG_001342"
     CDS             6519..7136
                     /locus_tag="AC1PNG_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HIP6701845.1"
                     /translation="MKIIKLFFSIKVYLWLVLSFLLLLCGVWGYGYISGEKSNQQEVS
                     IHTAIESVEQVNELVFLNTAVQKIVTAKNYTVLFGQEMPFSEKSALLIIKYKAKFGIK
                     SPVSIEHLSENRYKITLPAFKVIGLELDAEEPYTLYDKSGEILSFATEEIDTAQLITD
                     EFQSAEQETFLADYQDDIKESAKTYYQNLFSAISPEIQLEFVFKE"
     gene            7247..8269
                     /locus_tag="AC1PNG_001343"
     CDS             7247..8269
                     /locus_tag="AC1PNG_001343"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HIP6701846.1"
                     /translation="MTVNIDWEKLGFSYMKLPYRFIAHFKNGQWSEGQLTEDAELHIS
                     ESSPALHYGQQAFEGLKAYRTKEGKLQLFRPDQNAERLQRTADRLLMEPVPTDLFIKA
                     CKEVVKANADYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTVFAMPVGNYFKGG
                     LAPTNFLIQDKYDRAAPNGTGAAKVGGNYAASLLPGQYAKKQGFSDVIYLDPATHTKI
                     EEVGSANFFGITADNEFVTPISPSILPSITKYSLLYLAEHRLGMKAVEREVLVEELDQ
                     FVEAGACGTAAVISPIGGIQIADKLHVLHSETEVGPVTRKLYEELTGIQFGDIEAPEG
                     WIVEVD"
BASE COUNT         2441 a         1742 c         1925 g         2190 t
ORIGIN      
        1 ttatagcata ttttcatcta atttagtcca aaatcttatt ttgtgagttg tctcagccga
       61 taggtaacag aaatattttg caaaaatgct aaaaaacttg taatatagaa aggatgataa
      121 gtatggaggt caattttggc taagaaagag attgatataa ataattacaa tgatgatgcc
      181 attcaggtat tggaaggcct agatgccgtt cgcaaacgcc ctggaatgta cattggctca
      241 acagacggga atggtcttca ccacatggtc tgggagattg tcgataacgc tgtcgatgaa
      301 gccttgtctg gtttcggtga tcggattgac gtgactatta acaaggatgg tagcctgtct
      361 gtttccgacc gtggacgtgg gatgcctgtt ggtatgcacg caacaggcaa accaacagtc
      421 gaagttatct tcaccgttct ccacgcagga ggtaagttcg gtcaaggtgg ctacaagact
      481 tcaggtggtc tccacggggt tggttcttcg gtggtcaatg ccctgtctag ctggttggaa
      541 gttgagatta cccgtgacgg agctgtttac aagcaacggt ttgagcaggg cggaaagcca
      601 gtcacgaccc tagagaagat tggcaccgct ccaaaatcaa aaacaggtac aaaagttacc
      661 tttatgcctg acgataccat cttttcaacg acggacttca agttcaatac cattgccgaa
      721 cgcctgaaag aatcagcctt cttgctcaaa caagtcacca tgactctgac agatgagcga
      781 acaggtgagc aggaggagta ccactacgaa aatggcgttc aggactttgt ttcctacctc
      841 aacgaagaca aggaaactct gacaccagtc ctctattttg aaggagaaga tgcaggattc
      901 caagttcaag tggccatgca gtacaacgac ggttattcag acaacatcct gtcctttgtt
      961 aacaacgttc ggaccaagga cggcggtacc cacgaaacag gtctcaaact agccattacc
     1021 aaggctatga acgactacgc ccgcaagacc aacttgctca aggaaaagga caagaacttg
     1081 gaaggctctg actaccgtga aggtttgtct gcggtccttt ctatccttgt gccagaagag
     1141 catttgcagt ttgaagggca gaccaaggac aagttgggaa gcccacttgc tcgtccggtg
     1201 gtggacggca ttgtatcgga caagttaaca ttcttccttt tggaaaatgg ggaattagct
     1261 tcaaatctag ttcgtaaagc cattaaggcg cgtgacgccc gcgaggccgc tcgtaaggcc
     1321 cgtgacgaat cccgaaacgg taagaaaaac aagaaggata agggcctttt gtcagggaaa
     1381 ctcactccag cccagtccaa aaatccagcc aaaaatgaac tctatctggt cgagggagat
     1441 tcggccggag gttctgccaa acaaggccgt gaccgcaagt ttcaagccat tctacctttg
     1501 cgtggtaagg ttatcaatac cgccaaggca aaaatggcgg acatcctcaa aaacgaagaa
     1561 atcaatacca tgatttacac cattggtgca ggtgttggtt cagactttac gctggaagat
     1621 gtcaactatg acaagattat aatcatgacc gatgcggata cggatggtgc ccacattcag
     1681 actctcttgc tgactttctt ctatcgctat atgcgaccgt tagtagaagc aggacgggtt
     1741 tatatcgccc ttccgccact ttataagatg tcaaaaggca agggcaagac agaaaagatt
     1801 gcttatgctt ggtctgacgg agaattagaa gatctccgca aggattttgg caagggcttt
     1861 atcctccaac gctacaaggg tcttggtgag atgaatgccg atcaactctg ggaaacaacc
     1921 atgaaccctg aaacccgtac cctcatccgt gtcaccattg aagacctggc ccgtgctgaa
     1981 cgccgtgtat ctgtcctcat gggcgacaag gtcgagccac gccgcaagtg gattgaggac
     2041 aatgtgaagt ttaccttgga ggaagcgaca gcttttacta aataatctca gggaagttga
     2101 ggtgccatat ggttttagaa aataaattgg ggattgaaaa ttcggctgaa ttagctcgtc
     2161 ttgaagaaca aatcagcaag aaaaaagcag ctcaactgtt cgaaactggt cagttattcc
     2221 agatagaagt tggaaccttt gcaggtttgg cacacattca tcagaccttg tttgaagaga
     2281 tttacgactt ttcaggtaag attcgcgacg ttaatattgc caaggataat tttcaatttg
     2341 ctccacgaat ctttctggag cagtctttgg cctatatcga caagctacct catgaaacct
     2401 ttgacgaaat cgttgaaaaa tatgcagata tgaacattgc acatccattc cgagaaggaa
     2461 atggacgcag tatgcgtatc tggttggatt gtatgctacg ggatagtttg ggtaaggttg
     2521 ttgactggaa tagcattgat aaagatgaat atttcaatgc aatggttaga agccatgtgt
     2581 caacaggaga gcttaaatat ctgttattgc aagccttaac ggaagatctt ggtcaggcta
     2641 cttatttcaa aggcatcgac cattcttatt actatgaagg atacaatctc tatcgcacag
     2701 aggatttgta actctagcac aagcaaacta tgtgaggtgt tttatgccaa aattaactga
     2761 ccgttttcaa aaacggttgt ctgaatgggc tcgaaagcgc aagccattta aaaacaaaca
     2821 ggagttggaa gagaaatttg accaagcatt gaagatttgg caatcttcca aaactcggca
     2881 acatgcagaa aacttgctat ctgagcaagg aaaattagaa cattttttac atagcacgga
     2941 acggaaactg tctcgtatgc cttttggtgg agataagttt gcggccattc caggcttgat
     3001 ttccatggtg cgaagctata tccgtagaga ttacagcaag ttacccaaag ggacaatcct
     3061 cgcaattata ggtgctttga tttatttttt cagccctatt gatgccttac cagatttcat
     3121 ccttggagca ggtctacttg atgatgcctt tgtccttaat gcctgtttga aattaatcaa
     3181 aacagatgtc gatgacttta gaagctggca agcaagccag tggaaagtgg atgagtagct
     3241 atgataaagt ttttaagaaa aaagctaact atcgagcaac ttaagaaagt tccttatgct
     3301 tctcagtata cagaggttct acgttcaatt tggcgagcag atgtgcctaa atacgggatt
     3361 tcttctactt tacaaggtga attacttcga caattagaaa aacttcgctg ggaagctcaa
     3421 gcaaatggca atgtcaattg gtgtgaagag cactcaaact actgtcgatt cataaaggag
     3481 actctctata aaggaaaagt cctttcttct caacagaagc aagagttagt tttaataatg
     3541 gattatctaa aatcgtgtgg tgaatatgct caagcttatc aagaaaattt gattgatgat
     3601 gaggaattag aaattgagaa attagcatat gttgatgata atctttatga tagagtaggg
     3661 gatatgattg ctttctttta tcagagaact taggtaaaga actaatgttt agttaaggat
     3721 atagcaacag aaattgctat ataaggtctc ttccgttccc actacttaag gtgattatca
     3781 aaaaagtaaa aaagaaagaa aatttaactt tccaccaaga aacaaagaaa ggaatgttca
     3841 gctagtctgt ctaactgaac acgggactga atcgtccctt tgtattttaa tttatgtcaa
     3901 acattcaaaa catgtccctt gaggacatca tgggagagcg ttttggtcgc tattccaaat
     3961 acattattca ggagcgggcc ctgccggaca ttcgtgacgg tctcaagccc gtgcaacgtc
     4021 ggattcttta ttccatgaat aaggacggca acacttttga caagggttac cgcaagtccg
     4081 ccaagtcagt cggaaatatc atggggaatt tccacccgca cggtgattcc tctatttatg
     4141 atgccatggt ccgcatgagt caggactgga agaaccgcga gattttggtg gagatgcacg
     4201 gaaacaacgg ttccatggac ggcgatccgc cagcagccat gcgttacacc gaggctcgcc
     4261 tgtctgaaat ggctggttat ctgctggccg acatcgagaa aaagactgtg ccatttgcct
     4321 ggaactttga cgatacggaa aaagaaccca ctgtgctacc agctgccttc cctaatctct
     4381 tggttaatgg agcgacagga atttcagcag ggtacgcgac tgacatcccg ccgcataatc
     4441 tagcggaggt catcgatgcc gttgtctata tgattgatca tccaacagct aagttagaaa
     4501 agttgatgga gttcttgcct ggaccagact tcccaacagg tgccattatc caaggggctg
     4561 acgaaatcaa gaaagcctat gaaactggta agggccgtgt cgttgttcgt agccgttgtg
     4621 aaattgaaca acttaaggct ggcaagaaac agattgtcat tactgagatt ccttacgagg
     4681 tcaacaaggc tgttttggtc aagaagattg atgatgtccg tgtcaacaac aaggtgcctg
     4741 gtattgccga ggtgcgtgat gagtcagacc gtactggtct tcggattgcc attgagctga
     4801 aaaaagacag cgatgagcag accattctca actacctcta caaatacacc gacttgcaaa
     4861 tcaattacaa cttcaacatg gtggctattg ataatttcac accgcgtcag gttggcttgc
     4921 agaagatttt gtctagctat atcgcccacc gtcgtgagat tatcattgct cggtctaagt
     4981 ttgacaagga aaaggcagaa aaacgcctcc atatcgtaga aggtttgatt cgtgtcattt
     5041 ccatcttgga cgaggttatt gctcttatcc gtgcttctga aaacaaggct gacgctaagg
     5101 aaaacttgaa agtcagctat gattttagtg aggaacaggc agaagcaatc gtgaccttgc
     5161 aactctaccg cttgaccaat accgacattg ttaccttgga aaacgaagaa gcagccctac
     5221 gcgagcagat tcagaccttg gcagccatta tcggcgatga gcggaccatg tttaatctta
     5281 tgaagaaaga acttcgtgag gtcaagaaac aatttggcaa tcctcgtttg agtgagttac
     5341 aagatcaggc agaaacgatt gagattgaca ccgccagctt gattgtcgaa gaagaaactt
     5401 ttgtcagcgt gaccaaggca ggttatatca aacggacaag tccgcgttct ttcaatgcct
     5461 caacgttgga agaaatgggc aagcgggacg acgaccagct gattttcttg cagaatgcca
     5521 agacaaccca gcacctattg ctctttacca atcttggaaa tgtcatctac cgacctgtcc
     5581 atgagttgac agatattcgt tggaaggata ttggtgaaca cctgagccag actctcatga
     5641 actttgatac cagtgaagaa attatcttcg ctgaattggt ggagaatttt gaagagggaa
     5701 cttatttcgc ggtgacccaa tacggtcaaa tcaagcgtgt ggagcggaag gagtttactc
     5761 cttggcggac ttacaagtcc aagtcaacca agtatgctaa actcaaagat gcagaggatg
     5821 tggtcattac tgtatcgcct gtagtcttgg atgacatcat gctcatgaca gaaaagggct
     5881 atgctctgcg atttaacatc gaagaagtgc caatcatcgg tgccaaggcg gctggtgtca
     5941 aggcggtcaa cctcaaggat ggagatgtgg tggttgcggc ctttatcagc aacacaagct
     6001 ctgtctatct tctgacccag cgtggtagct ttaagcggat ggcgacggag gaaatccctg
     6061 tgaccagccg tgccaagcgt ggtttgcagg tgcttcgcga actcaaggcc aagcctcacc
     6121 gtgtctttgc ggcgggtcca gtcttgacgg accaggggga ttttgacctc tttagcacag
     6181 caaatgaaga tgaaacgaca agtcaaatcc ttcacgtgca gtccaaaaca ggcaaactct
     6241 atgaggttga tgtgacccag cttagcctgt ctgaacgcac tagcaacggc agcttcattt
     6301 cggataccat ttccgatgaa gaagtcttca gagcctggat tgattaggta tttagaatga
     6361 aaaagaaatt ccttattttg ttcactatct tagtactgat agtccttgcc atgaaagtct
     6421 ttcctacctt tgaggactgg cttcaccagc tgactggttg gtactaatcg aggcctgtaa
     6481 agcagtcttt ttgctatact gtagaggagg taaatgctat gaaaattatc aaattatttt
     6541 tcagtataaa agtctatcta tggcttgttc tgtcctttct tctgttattg tgtggtgtat
     6601 ggggatacgg ctacatttct ggcgaaaaga gtaatcagca agaagtgagt atccatacgg
     6661 ctattgaatc tgtcgagcaa gtcaatgaat tggtatttct aaatacagct gtccagaaaa
     6721 ttgtgaccgc taaaaactac acggtcttat ttggtcaaga aatgcccttc tcagaaaaat
     6781 cagccctctt aatcattaag tacaaggcaa aatttggcat caaaagtcct gtatccattg
     6841 aacatctttc tgaaaatcgg tacaaaatta cccttccagc ctttaaagtc attggattgg
     6901 aattagatgc agaagaacct tataccttat atgataagag tggtgagata ctgagttttg
     6961 cgacagaaga aatcgatact gcccagctca tcacagatga atttcagtca gcagaacaag
     7021 aaacgttttt agcagattat caagatgaca taaaagagtc agctaaaacc tactatcaaa
     7081 atttatttag tgctatctca cctgaaattc aactagaatt tgtttttaaa gagtaagagt
     7141 ctggtttacc aggctttttc ttatattcac aaaattttct gaatttttaa ttgaaaatca
     7201 ctttcaaaag tagtaaaata ataaaaaatg aatttggaga aactatatga cagtgaacat
     7261 tgactgggaa aaacttggtt tttcttatat gaaactacct tatcgcttca tcgctcattt
     7321 caaaaacggc cagtggtctg aggggcagct gaccgaagat gcagaacttc acatttcaga
     7381 aagctcccca gcccttcact atggccagca ggccttcgag ggccttaaag cctatcgcac
     7441 caaggaggga aagctccaac ttttccgtcc agaccaaaac gctgagcgcc tacaacggac
     7501 agctgaccgc ttgctcatgg agcctgtacc gactgacctt ttcatcaagg cttgtaaaga
     7561 ggtggtcaaa gccaacgcag actacgttcc tccctatgga acaggtggca ccctttatct
     7621 ccgtccgctc ctgattggcg tcggcgacat catcggcgta aaaccagcag aagagtacat
     7681 tttcacagtt tttgccatgc cagttggcaa ctacttcaag ggtggtttgg ctccgaccaa
     7741 cttcctcatt caggacaagt acgaccgtgc ggcaccaaat ggaacagggg cagctaaggt
     7801 tggtggtaac tacgcggcat cgctcttacc gggccaatat gccaaaaagc agggcttctc
     7861 agatgtcatc tacctggacc cagctaccca caccaagatt gaagaagtag ggtctgctaa
     7921 tttctttggt atcacagctg ataacgagtt tgtcacaccg atttcaccat caatcttgcc
     7981 gtccattacc aagtattctc tcttgtattt ggcagagcat cgtctgggta tgaaggctgt
     8041 ggagcgtgaa gtccttgtcg aagaattaga ccagtttgtc gaagcagggg cttgcggaac
     8101 agctgcagtc atctcaccaa ttggtggtat ccagattgca gacaaactcc atgttctcca
     8161 tagtgaaaca gaagtaggac ctgtcactcg taaattgtat gaagaattaa caggtatcca
     8221 gtttggtgat attgaagctc ctgaaggctg gattgtcgaa gtagattaat ttcagaaggc
     8281 ttgcacttgc aagctctt
//
LOCUS       DBNFEG010000092         8199 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.092, whole genome shotgun sequence.
ACCESSION   DBNFEG010000092 DBNFEG010000000
VERSION     DBNFEG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8199
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.092"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(470..718)
                     /locus_tag="AC1PNG_001344"
     CDS             complement(470..718)
                     /locus_tag="AC1PNG_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HIP6701847.1"
                     /translation="MFYLILAIMLVLFYIFIAPRNIKGTMNLIAAVFLLVALAIALVL
                     GFLRIMQFPKEIWVGLLMILIGFWAMRDIYQLNDDTKF"
     gene            complement(736..2583)
                     /gene="typA"
                     /locus_tag="AC1PNG_001345"
     CDS             complement(736..2583)
                     /gene="typA"
                     /locus_tag="AC1PNG_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIP6701848.1"
                     /translation="MTKLREDIRNVAIIAHVDHGKTTLVDELLKQSQTLDERTQLDER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLTPIVVVNKIDKPSARPEEVVDEVLELFIELGADDDQ
                     LEFPVVYASAINGTSSLSDNPADQEETMAPIFDTIIEHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERKEIQEAKAG
                     DLIAVSGMEDIFVGETVTPTDAVEALPVLRIDEPTLQMTFLVNNSPFAGREGKWVTSR
                     KIEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVKMEPFERVQIDTPEEYQGSVIQSLSERKGDMLDMVATGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIDQGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITTAKQMTNVRSATKDQTSVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAERDKAAKKKKLATEE"
     gene            complement(2716..3708)
                     /gene="asnA"
                     /locus_tag="AC1PNG_001346"
     CDS             complement(2716..3708)
                     /gene="asnA"
                     /locus_tag="AC1PNG_001346"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HIP6701849.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEIVEVQGPILSRVGDGM
                     QDNLSGVENAVTVNVKLIPDATYEVVHSLAKWKRHTLARFGFHEGEGLFVHMKALRPD
                     EDSLDPIHSVYVDQWDWEKVIPDGRRNLAYLKETVEQIYKAIRLTELAVEARYDIESV
                     LPKKITFIHTEDLVKNFPDLTPKERENVVAKEYGAVFLIGIGGELADGKPHDGRAPDY
                     DDWTSPSEAGYKGLNGDILVWNEALGSAFELSSMGIRVDEEALKRQVAITGDEDRLEF
                     DWHRALLNGLFPLSIGGGIGQSRLAMFLLRKKHIGEVQSSVWPQEVRDNFENIL"
     gene            complement(3793..4842)
                     /locus_tag="AC1PNG_001347"
     CDS             complement(3793..4842)
                     /locus_tag="AC1PNG_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HIP6701850.1"
                     /translation="MSRQIAHLPSLIPFGFILADNRYTYREVFMEGQFEAVVEVDEAG
                     QLSSYVWDCEMEEVYTAHLVTAPAGAFVGQVREAYQSILARVEEVCCIALPFSKDQSN
                     RIAQLIKEKWGDLPDYPFAKLPTYGAFRHPSNNKWYALVSQIPRDKLDGSGSKEEVEI
                     VNLKVDGREIAELLSQSGIFPAYHMSKKSWVSVLLDETVEDQVVFALLEKSRYLVGPK
                     SYKAEQGSDYWVIPANPKVYDIDTEFAENKVVYWPQKSTIQAGDIVAIYVTAPVQAIR
                     YVCRVLGANLENHGESDIPTGKKLMQVELLAQFSDDVLQRARMMDLGVRAVRGPRRLT
                     EGVIEVLTSEVKNLH"
     gene            4917..5309
                     /locus_tag="AC1PNG_001348"
     CDS             4917..5309
                     /locus_tag="AC1PNG_001348"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HIP6701851.1"
                     /translation="MSKKIISVVAAAIEKDGKIFCAQRPEGKSLGGYWEFPGGKLEAN
                     ETPEEALVREIKEEFDSEIEIVSFINEASYEYDFGIVTMKTYLSKLVTGDLTLLEHQD
                     SKWLPIEDLETLEWAPVDIPAVKCLQQK"
     gene            complement(5333..>8199)
                     /locus_tag="AC1PNG_001349"
     CDS             complement(5333..>8199)
                     /locus_tag="AC1PNG_001349"
                     /EC_number="3.1.4.4"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004630 - phospholipase D activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIP6701852.1"
                     /translation="NNLFQDQLLHSLQYGFIDREIFQEGVYSPKILINDTETHRYVLN
                     DIQEELSKAQSFAFSVAFITQSGLALIKSQLSDLADEGIQGRILISPYLDFNDPIAMK
                     ELLKLKNVQVRITPVNLQMHAKFYLFEQKDKQVIISGSSNLTHTALKINYEWNVKLTS
                     THNGDFIQNAKEEFDRIWEKSIVLDLEAINAYSARRQTVIKTAMIRDESIASYQETIR
                     PNKMQEQALEGLATVRKNGAQKALVISATGTGKTYLSAFDVKQYNPDRMLFIVHREQI
                     LQKSLKDFQKVLGFEDDEGCIYKSGMDISQKRYIFATIQTISRADNLQAFDPTFFNYI
                     LIDEVHKAGADSYKKVMNYFQPDFYLGMTATPERTDGQNIYELFDYNIAYEIRLQEAL
                     DNDMLCPFIYYGVSDIKIDGQLIDEKATFSNLVSEQRVNHILEKIAYYGVSGEAVKGL
                     IFCSSVNEAKELEGQFNARGLRTRALSGEHSQKERQRVVQDLENGNLDYILTVDIFNE
                     GIDIPSVNQVVMLRNTQSSIIFIQQLGRGLRKHDSKEYVTIIDFIGNYQNNYLIPVAL
                     FGDQSMNKDNYRREVREPNILKGLTTINFEKVAKEQIFSSITNTTLSSIKILRDAFKD
                     LENKLGRVPYLSDFIKLNSIDPLVFFENSSFQNYGDVINKFSEETILLSEVEKRFLNF
                     ITFEILNGKRKHELYLLKLLVENEGLVSSDKWFECIIEHDLDFQTDVLDSVTRVLDLS
                     FLKAQEQKKYGTDALVTFDGELYRLNEEIQESLVNNTKFTQLFLDVIETGILKSKDYP
                     EIFTLGQKYTRREVLKLMNVAKDEPPLNIGGYKIDKDTNSCPIFITYHKSEDISDTIK
                     YDDELLNESTLKWFSKNKRTLDSNDVSTITQSNENGLRLELFVIKDDSEGGEFYYLGP
                     LSYVENSASEVMKNGDSVVQMLFKLDSPVETNLYKYLTDK"
BASE COUNT         2423 a         1787 c         1517 g         2472 t
ORIGIN      
        1 tcaattatag agatcagttt cagactgtaa aacacgaatt gctaagatgt tgacaaaagt
       61 tggcggcggc agctcattgc cttgtgtggt cgaatgaatt agctttcaga caaggaactg
      121 aggtgcaggt agctagaaca gcctagaggc tgttctaggt tggaaataag aattgcgaaa
      181 caagtcactt tcgtagagta cgacaagccg aaagcggcga tgtatcagag ttaatttaaa
      241 tgactgtact aagaacttgc tattttgggg atttcattga gtattaaaat ttatctacat
      301 aaaaacacct tgaaaatgcg cttgtcattt attataaata acgagagctt cttcaagatg
      361 tatcacagaa tataatttct ttagctaata gattgccact actgtccaaa aagctggata
      421 aaattcctta gtaatatcgt attacggtat tcaaaaatca aagactctgc taaaattttg
      481 tatcgtcatt taattgataa atatcccgca tcgcccaaaa accaatcaga atcattagta
      541 gaccaaccca aatttccttt ggaaattgca tgattcttaa aaatccaagt accaaggcaa
      601 tcgctaatgc taccagtaaa aatactgccg caatcaaatt catagtcccc ttaatattgc
      661 gaggtgcaat aaaaatataa aaaagtacta acatgatggc gaggattaaa taaaacatgc
      721 ttcttctcct agaaattatt cctcagtcgc taattttttc ttcttcgcag ccttatcacg
      781 ctctgccttg ttcaagattt gcttacgaag acggatagat tctggcgtta cttccatgta
      841 ctcatcatcg ttcaagaatt ctagtgattc ttcaagagtc aagatacgtg gtgtcttaat
      901 tacagacgtt tggtctttgg ttgctgaacg aacgttggtc atttgtttag cagtcgtgat
      961 gttaacggtc aagtcatttt cacgcgagtt ttcaccgata atcattcctt cgtacacttc
     1021 agtacctggg tttacaaaga tggtaccacg ctcttcaata cgcatgatag agtatgtcgt
     1081 tgccttacct tgatcgatag aaacgagagc accacggtga cgaccaccga tttcaccttg
     1141 aacaactggc aagtattgat caaaggtatg gttcataata ccgtaaccac gagtcattga
     1201 aaggaattct gttgaataac caatcaaacc acgtgcaggt acgaggaaga caagtcgtgt
     1261 ttgaccatta cctgtcgcaa ccatatccaa catatcaccc ttacgctcag atagggattg
     1321 gataacagaa ccttggtatt cttctggtgt atcaatttgc acgcgctcaa atggctccat
     1381 tttcacgccg tcgatttcct tgatgataac ttctggacgt gatacttgaa gctcataacc
     1441 ctcacgacgc atggtttcaa tcaagataga caagtgcaat tcaccacgac ctgaaacagt
     1501 ccatttatct ggagagtcag tagggtcaac acggagagaa acgtctgttt gcaactcggc
     1561 caaaaggcgc tcttcaatct tgcgagatgt cacccattta ccttcacgac ctgcaaacgg
     1621 tgagttgttc accaagaaag tcatttgaag agttggctca tcgatacgaa gaactggaag
     1681 tgcctcaact gcatctgtcg gagtcacagt ttcaccaacg aagatgtctt ccataccaga
     1741 aacagcaatc aaatcaccag cttttgcttc ttggatttct ttacgctcaa gaccgaagaa
     1801 accaaagagt tttgtaacgc ggaagttttt cgttgtaccg tctagtttag aaagggtaac
     1861 ctggtcacca actttaacag taccacggaa gacacgaccg ataccgatac gacctacgaa
     1921 gtcattgtag tccaaaagcg atacttggaa ctgcaaaggc tcatctgagt tatcgactgg
     1981 cgctggaata tgctcgataa tggtatcaaa aattggtgcc attgtttctt cttggtctgc
     2041 tggattgtca gacaaagatg acgttccgtt gatagctgat gcataaacca ctgggaattc
     2101 caactggtca tcatctgcac ccaattcgat gaagagttca agcacttcat ccacaacttc
     2161 ttctggacga gcagaaggct tgtcaatctt gttaacaacg acgattggtg tcaagttttg
     2221 ttcaagggct tttttcaata cgaaacgagt ctgtggcatg gtaccttcgt aagcatctac
     2281 tacaaggaca acaccgtcaa ccattttcat gatacgctca acctcaccac cgaagtccgc
     2341 gtgtcctggt gtgtccatga tgttgatacg agtaccgttg taagctacgg cagtgttttt
     2401 agccaggatt gtgataccac gctctttttc gatatcgttt gagtccatgg cacgctcatc
     2461 caactgcgta cgttcatcaa gtgtttggga ttgtttcaat aattcatcaa cgagggttgt
     2521 ttttccgtgg tcaacgtggg caataatggc aacgttacgg atgtcttctc taagttttgt
     2581 cataatatcc tctatatatt ttaatttaac taaaaaagta tatcatagat tggcgcaaaa
     2641 atcacagata atcttgttgt aaatgttttc agatagcaga ttgaaagcag aagaaaagag
     2701 attgaagcca atctcttata aaatattctc aaaattatcg cgcacttctt gtggccaaac
     2761 gctggactgc acttcaccaa tatgtttctt acgaagcaag aacatggcca gacgggattg
     2821 accgataccg ccaccgatag acagcgggaa gagaccattc aaaagggcac gatgccaatc
     2881 aaattccaaa cggtcctcgt cacctgtaat agcaacctgg cgcttaagcg cttcctcgtc
     2941 tacacgaatc cccatcgagg acaactcaaa ggctgaacca agtgcttcgt tccaaaccaa
     3001 gatgtctcca ttgagtcctt tgtagcctgc ttctgatggg cttgtccagt catcatagtc
     3061 tggtgcacgt ccatcatgtg gtttcccatc tgccagttct ccaccaatac cgattaggaa
     3121 aaccgcaccg tattctttgg caaccacatt ttcacgctct tttggtgtca agtctgggaa
     3181 gttcttcacc aagtcctctg tgtgaataaa ggtgattttc tttggtaaaa cagactcaat
     3241 gtcataacga gcttcaacgg ccaattcggt cagacggata gccttgtaaa tctgctctac
     3301 tgtttctttc aagtaagcta aattccgacg accatcaggg atgacctttt cccagtccca
     3361 ctgatccaca tacaccgagt gaatcggatc caaggaatcc tcatctggac gcaaggcttt
     3421 catatgtaca aaaagacctt ctccttcgtg aaatccaaag cgagccaagg tatgacgctt
     3481 ccattttgca agcgaatgaa ccacttcata agtagcatct ggtatcagct tcacattaac
     3541 ggtaactgca ttctccacac cggacaaatt atcctgcata ccatccccga cacgactcaa
     3601 aatcggccct tgcacttcta cgatttctag cttgtctttc aaatactgag tgaatgtatt
     3661 tttgacgaaa gaaatctcct cttgctgatg aatgaagctt ttcttcatac cttacctcct
     3721 cgtaaattta tacaccttat tatactcttt tttacccact tgtaaagtct ttttattaaa
     3781 tattctgata atttaatgga ggtttttcac ttcggatgtc agtacttcta tcacgccctc
     3841 tgtcaagcgt ctaggaccac gtaccgccct gactcccaag tccatcatgc gcgctctctg
     3901 caaaacatca tcggaaaact gagcaagcaa ttccacctgc atgagtttct ttcctgttgg
     3961 aatatctgac tctccatgat tttccaagtt tgcccccaag actcgacaaa catagcgaat
     4021 ggcctgtaca ggtgccgtga cataaatggc aacgatgtcc ccggcttgaa tggttgattt
     4081 ttgtggccaa tagactacct tattttccgc aaattcggta tcgatatcat agaccttggg
     4141 attggctgga ataacccagt aatcagaacc ttgctccgcc ttgtaggatt ttggtcctac
     4201 aagatagcgg ctcttctcaa ggagggcaaa caccacctgg tcctccacag tctcatccag
     4261 caagaccgac acccaagact tcttagacat gtgataggct gggaaaatgc ccgactgact
     4321 tagcagctct gctatttccc gaccatcgac cttgagattg acaatctcca cctcttcttt
     4381 agacccgctc ccgtccagct tgtccctcgg aatctgactg accagggcgt accacttatt
     4441 attggagggg tggcgaaaag ccccataggt cggcaacttg gcaaagggat agtcaggaag
     4501 gtccccccac ttctccttga tgagctgggc tatgcggttg ctttggtctt tggaaaatgg
     4561 cagagcgata cagcagactt cctctacccg agccaaaatg gactgataag cctcacgcac
     4621 ctgccccaca aaagcccctg ctggcgcagt caccagatgg gcggtataga cctcctccat
     4681 ctcacaatcc cagacatagg acgacagctg accagcctca tcaacctcca ccaccgcctc
     4741 aaactgcccc tccatgaaga cctcacggta ggtgtaacga ttgtcggcga gaataaaacc
     4801 gaaagggatg agggagggga ggtgggcaat ttgtctagac attttaactc ctttaaagta
     4861 atatatagag aaagtataac aaaatctgta aaagaattta ttgtggaggt caaatcttgt
     4921 caaaaaaaat tattagtgtg gtagctgcag caattgaaaa agatggaaaa atattttgcg
     4981 ctcagcgccc tgaaggaaag agtttaggag ggtattggga atttccagga ggaaaactcg
     5041 aagccaatga aactcctgaa gaagccttag ttcgtgaaat caaagaagaa tttgattccg
     5101 aaatcgaaat agtatctttc atcaacgaag catcttatga atatgatttt ggtatcgtga
     5161 caatgaaaac ctacctttca aaattagtca cgggtgattt aactttatta gaacaccagg
     5221 attctaaatg gctccccatc gaagatttag agacattaga atgggctcca gtagatattc
     5281 ctgccgtcaa atgtttgcaa caaaaataga gattcgctgg aatctctatt ttttatttat
     5341 ctgtcaaata cttatacaag tttgtctcca ctggtgaatc caacttaaat aacatttgga
     5401 caactgaatc tccatttttc attacttcac ttgcactatt ttcaacgtat gatagtggac
     5461 caagataata aaattctcca ccttctgaat catcttttat gacaaaaagt tctaacctca
     5521 atccattttc attagattga gtaatcgtcg atacatcatt agaatctaat gtacgtttgt
     5581 tctttgaaaa ccacttcaaa gttgactcat ttagaagttc gtcatcgtat tttatggtat
     5641 cactaatatc ctcactttta tggtaagtga tgaaaatcgg acaagaattg gtgtccttat
     5701 caattttgta tccaccaata tttaaaggtg gttcgtcttt tgcgacattc attaatttaa
     5761 gcacttcacg tcgagtatat ttctgaccaa gtgtgaaaat ttcaggataa tctttcgatt
     5821 ttagaatccc tgtttcaatg acatccaaaa ataattgagt gaatttggta ttattcacca
     5881 aagattcttg gatttcttca ttcaaccgat acagttcacc gtcaaaagta accagtgcat
     5941 ccgtaccata tttcttttgc tcctgggctt ttagaaatga taggtccaac actcttgtta
     6001 ccgaatctaa aacatctgtt tgaaaatcta aatcatgctc aataatacat tcaaaccatt
     6061 tatctgacga aactagtccc tcattttcga caagcaactt caacaaatac agttcatgtt
     6121 ttcgtttgcc attcaagatt tcaaatgtaa taaaattcaa aaatcgcttt tctacttctg
     6181 atagaaggat tgtttcttct gaaaatttat tgatgacatc tccgtaattt tgaaaactag
     6241 agttttcaaa aaagacaagc ggatcaatgc tgtttaattt tatgaaatct gatagatacg
     6301 gaactctacc caacttattc tctaagtctt taaaagcatc tcgtaaaatt ttgattgatg
     6361 agagtgtcgt atttgtgata gaagaaaaaa tttgttcttt ggcaaccttc tcaaagttga
     6421 ttgtcgtcaa tccttttaaa atgttgggct ctctgacctc acgacgataa ttgtctttgt
     6481 tcatagactg atcgccaaat agtgcaacag gaattagata gttgttttga tagtttccaa
     6541 tgaaatcaat aatcgtaaca tattctttcg aatcgtgctt tcgcaagcct cgacctaatt
     6601 gttgaataaa aatgatactc gattgggtat tgcgaagcat gacaacctgg ttgacactcg
     6661 gaatatcaat tccctcatta aaaatatcaa ccgtcagaat atagtctaag tttccatttt
     6721 ctaagtcttg aactactctc tgtcgttcct tttggctatg ttccccagaa agagcgcgtg
     6781 tacggagtcc tcttgcatta aactgcccct ctagctcttt tgcttcattt actgaagaac
     6841 agaaaatcaa tccttttacc gcctcaccac ttactccata gtaagcaatt ttttctaaaa
     6901 tatgattgac acgttgttca gaaactaaat ttgaaaaagt ggctttttca tcaattaatt
     6961 ggccatcaat cttaatgtct gaaacaccgt agtatataaa tggacaaagc atatcattat
     7021 caagtgcctc ttgtaaacgg atctcatagg caatattata gtcaaaaagc tcataaatat
     7081 tttgtccatc tgttcgctct ggtgttgctg tcatccctaa ataaaaatct ggctgaaaat
     7141 aattcatcac ttttttatag gagtctgcac ctgctttatg aacctcatca atcaaaatat
     7201 agttaaagaa agttgggtca aaagcttgaa ggttatctgc tctcgaaatc gtctgaatag
     7261 tcgcaaagat atacctcttc tgtgatatat ccatcccact cttataaata cagccctcat
     7321 catcctcaaa tcctaacacc ttttgaaagt cttttaatga tttttgtagg atttgttctc
     7381 ggtggactat aaataacatt cgatctggat tgtattgttt gacatcaaag gcagaaagat
     7441 aggttttacc tgttccagtt gcactaatga ccagagcttt ttgtgcacca tttttcctaa
     7501 cggttgcgag accttctaga gcttgctctt gcatcttgtt gggacgaatg gtttcttggt
     7561 aagatgctat tgactcgtcc cgaatcatcg ctgtttttat caccgtctgc ctacgagctg
     7621 aatatgcatt gattgcttct aggtctaaaa caattgattt ttcccaaata cgatcaaact
     7681 cttcttttgc attctggata aaatcaccgt tatgtgttga agtcaatttc acattccact
     7741 catagttaat tttcaaggct gtatgagtta aatttgaact accagaaatg ataacctgtt
     7801 tatctttttg ctcaaacaga taaaactttg catgcatttg caaattgact ggtgttatcc
     7861 gtacttggac atttttcagt ttaagcaatt ctttcatcgc tatgggatcg ttgaaatcca
     7921 gatacggaga aatcaaaatt ctcccttgga tcccctcatc cgccaaatct gataattgac
     7981 ttttaattag ggctagtcca gactgagtaa taaaagccac actgaaggca aaagattggg
     8041 ccttgcttaa ctcttcttga atatcattta aaacatagcg atgagtctct gtatcattta
     8101 ttaaaatttt aggcgaatag accccttctt gaaaaatctc tctatcaatg aaaccatatt
     8161 gcaaagaatg aagaagctgg tcttggaata agttgttgt
//
LOCUS       DBNFEG010000093         8170 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.093, whole genome shotgun sequence.
ACCESSION   DBNFEG010000093 DBNFEG010000000
VERSION     DBNFEG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8170
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.093"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            181..936
                     /gene="ldcB"
                     /locus_tag="AC1PNG_001350"
     CDS             181..936
                     /gene="ldcB"
                     /locus_tag="AC1PNG_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase LdcB/DacB"
                     /protein_id="HIP6701853.1"
                     /translation="MIMKRTILLCLPTILLLAACHQNINQANTTKNSSTVVSTTSSQS
                     ASTTSEQSTSSSTSAETNQEVTSTETQVNFNGSYYSVQGKYGEVIIVNKKHPLAADYA
                     PGENPEALAAFQRLIADMQALGFGISNNYSGFRSYETQASLYQSYVNQEGQAAADRYS
                     ARAGYSEHQTGLAFDLLDTSGNLLEEPNASQWLATNAHLYGFIVRYLPGKESSTGYMA
                     ESWHVRYIGQEATDIYNSGLTLEEYFGVPGGDY"
     gene            1025..1481
                     /locus_tag="AC1PNG_001351"
                     /pseudo
     CDS             1025..1481
                     /locus_tag="AC1PNG_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1504..3114)
                     /locus_tag="AC1PNG_001352"
     CDS             complement(1504..3114)
                     /locus_tag="AC1PNG_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HIP6701854.1"
                     /translation="MSKSIIWELVKINILYSNPQLLASVKKKQSKRKDASFSAYKSVL
                     LQQVFMVLSFGLLYSVFFMGVDYSQSIGFFSLQLALFTIIAIVYGFTGFFSVFYDSKD
                     TKLYLALPLKPQEVFLAKILSAQGMVLPFLMPCLSLLLITYWQIGGPLLALASIPSFL
                     ILWILANLVNLIIMHFVGEVLTKSPRKTLISTILMTGSSLLAFAALMFLQSQQTVSLE
                     SGGFVDFPIIPIFAGFHYLVSHTISLETLLHFGLPFILMIGLIVFAFKTVIPNYFNQV
                     LAIENASTKRTNNAKSAKIPSNLNQALVKHHLSTLKDSNLMVQTMIQPIIMGVALFPS
                     FSRFTENGGLSSVEWDFFGIALLAGFLLGSLFAGSTTFIGVAMSLEKENYHFIRTLPI
                     NFKKFIIQKFWVLAAVQFALPTLLYFALALGFMKVKLILAIFFTVGIIISALLTGQIY
                     YWRDQRLLMLNWQNSNQLFGRGAGQWLIAGSIIGTMFLGAIILVVSIILAGTIGAAYV
                     SSALMTLLFVLSAALQLYIYKAYWQKLK"
     gene            complement(3111..3854)
                     /locus_tag="AC1PNG_001353"
     CDS             complement(3111..3854)
                     /locus_tag="AC1PNG_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6701855.1"
                     /translation="MIQFDHITKTYGETIALDDLNLTIESGEIFGLIGHNGAGKTTTI
                     SLLTSIIEASYGTISVDGLNLEENRDEVKKRIAYVPDSPDIFLHLTAFEYWHFMGKVY
                     GVEKDTVDDRIAKLSSLFDMTAHQHEPIDSFSHGMRQKTIIIGALIPNPDIWILDEPL
                     TGLDPQASFDLKQMMKEHAQSGHTVLFSTHVLSVAEQLCDRIAILRKGQLIFVGSLDE
                     LKSQYPDKDLETIYLELAGRQQVKAGEEV"
     gene            4094..5878
                     /gene="uvrC"
                     /locus_tag="AC1PNG_001354"
     CDS             4094..5878
                     /gene="uvrC"
                     /locus_tag="AC1PNG_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIP6701856.1"
                     /translation="MNDLIKHKLELLPDSPGCYLHKNKYGKIIYVGKAKNLRNRVRSY
                     FRGAHDTKTEALVAEIADFEFIVTDSNIEALLLEINLIQENKPRYNIMLKDDKSYPFI
                     KITKERHPRLIITRQIKKDGGQYFGPYPDVGAANQTLKLLERLYPFRKCKLPENKYCL
                     YYHLRQCLAHGDNMPSLSDYDKMVKEVAQFLTGHDDKIIKEVQDKMKAAAGNLEFEKA
                     AEYRDVLQSISTLRTKQRVMAHDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERNVNL
                     FPYYNDADEDFLTYIGQFYRDQQHLIPSEVLIPQDIDQEAVEALVDTKVIKPQRGEKK
                     QLINLATKNARVSLEQKFNLLEKNLEKTYGAVENIGQLLGIPTPVRIEAFDNSNIMGT
                     SPVSAMVVFENGVPNKKEYRKYKINTVEGPDDYASMREVLQRRYSRVLRDDLTPPDLI
                     IIDGGQGQVNVAKQVIEQELGMSIPIAGLQKNDKHQTHELLFGNPLEVVELPRNSQEF
                     FLLHRIQDEVHRFAIEFHRQVRSKNSFSSKLDGIEGLGPKRKQALMKHFKSIGNIQSA
                     SLQEIAEAGVPYKVAEKVKESLSSKDEI"
     gene            5935..6063
                     /locus_tag="AC1PNG_001355"
     CDS             5935..6063
                     /locus_tag="AC1PNG_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HIP6701857.1"
                     /translation="MAFGEEKHKKTTFEKVTLVIVVVMVVATLAGLILPAINALMN"
     gene            6267..7580
                     /gene="obgE"
                     /locus_tag="AC1PNG_001356"
     CDS             6267..7580
                     /gene="obgE"
                     /locus_tag="AC1PNG_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIP6701858.1"
                     /translation="MSMFLDTAKIKVKAGKGGDGMVAFRREKYVPNGGPWGGDGGHGG
                     NVVFVVDEGLRTLMDFRYNRRFKADDGEKGMTKGMHGRGAEDLIVRVPQGTTVRDADT
                     GKIITDLVENGQEFVIAHGGRGGRGNIRFATPKNPAPEISENGEPGEERNLELELKVL
                     ADVGLVGFPSVGKSTLLSVITAAKPKIGAYHFTTIVPNLGMVRTKSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVLDMSASEGRDPYEDYVAINNELETYNLRLME
                     RPQIIVANKMDMPEAAENLEEFKKKLAANYDEFEELPQIFPISGIAHQGLKNLLEATA
                     ELLEKTPEFLLYEESDFQEEEAYYGFNPDEPEFDISRADDASWVLSGDKLEKLFTMTN
                     FDRDESVMKFARQLRGMGVDEALRARGAKDGDIVRIGKFEFEFVD"
     gene            7954..8118
                     /locus_tag="AC1PNG_001357"
     CDS             7954..8118
                     /locus_tag="AC1PNG_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701859.1"
                     /translation="MGDKPISFKDKDGNFVSAADVWNAEKLEELFNKLNPNRKLRLER
                     ERLAREQEKE"
BASE COUNT         2513 a         1516 c         1883 g         2258 t
ORIGIN      
        1 aaggctggtg tttctattta tgacatgtat gttatcagga atgccactat ttgaaaggaa
       61 aagaatcgag agaaattcaa tgaatactaa aaatatcaat agtagttaaa atcataagcc
      121 gtcattagac ggggtcttat gattgtgcta caatagacat aattataaga gaacggggtg
      181 atgataatga aacgtactat tctattatgc ttgccgacaa tactgttact agcagcatgt
      241 catcaaaata taaatcaggc caatactaca aaaaattcgt caacagttgt ttcgactact
      301 tcaagtcagt ctgcttcaac aacttcggag cagtcaacaa gcagctctac atcggcggaa
      361 acgaatcaag aagtaacaag tacagagact caggtaaatt ttaacggttc ctattatagt
      421 gtacaaggaa aatatggcga agtgattatt gtaaataaaa agcatccgct tgcagctgat
      481 tatgcaccgg gcgaaaatcc tgaagcctta gcagcttttc aacgattaat agcagatatg
      541 caggcgctag gatttggtat ctccaataac tatagtggat ttcgttccta tgaaactcag
      601 gcaagtctct accagtctta tgtaaatcag gaaggacaag cagcggcaga taggtattct
      661 gcaagagctg gatatagtga acatcagaca ggattagcct ttgatttatt ggatacaagt
      721 ggtaatttat tagaagagcc taatgcttct cagtggttgg ctacgaatgc acatctatat
      781 ggctttattg tccgctatct tccaggaaaa gagtctagca caggctatat ggcagaatcc
      841 tggcatgtgc gatatattgg tcaagaagcg acagatattt acaattcagg cttgaccttg
      901 gaagaatatt ttggtgtacc aggtggagac tactaatttt acatttagtc agaaataagc
      961 cgaaaaattt gtccgtataa ctaactcgtg atataataaa ccatcataat tttggaggtc
     1021 catcatgctt aaagcaatgg aagtatactt ggttactaat ggtaatggtt tggaagccat
     1081 cgagttttat aaaaatgctc ttggggcaac agttgagcaa gtaaatctat ttaaagattt
     1141 cttaccagac tgccctgctg aactagaaaa ttacgtgatg aatgcacagt ttcgtttgaa
     1201 tggtcagcgt tttatgttgt cagacaacaa cccagaaatg ccttatacag taggtgacaa
     1261 tattacagtt gcgcttatca cagacgatgc tgagacagcg caggaactat acagcaagtt
     1321 atccgtagat gcaactgcta tcaatatgga acttcaagat ggaacttcaa gcagttcctt
     1381 ggtctcctgc ctatggtaat gtaacagaca agtttggtat ctcttggcaa atcaatgctg
     1441 aagtagaagg ctacggtcag gaatattacg cagaaaacta aaatagattg gacaatgtcc
     1501 aatctatttt agtttttgcc aataagcttt gtagatataa agttgaagag cagcagatag
     1561 aacaaatagt aaggtcatca aggctgagct tacataagct gcaccaatag tgccagctaa
     1621 tatgatgctg acaactagaa taatggctcc gagaaacatt gttcctatga tcgatccagc
     1681 aattagccat tgacccgctc cgcgaccaaa taactggtta ctattttgcc agttgagcat
     1741 gaggagtcgt tggtcacgcc agtaataaat ctgaccagtt agcagggctg aaatgatgat
     1801 gccgactgta aagaagattg ctagaatgag tttgactttc atgaaaccta gagccaaagc
     1861 aaagtaaagt agagttggta gtgcgaattg tacggcagcc aatacccaga atttttggat
     1921 gataaatttt ttgaaattta taggaagtgt tcgaatgaaa tgataatttt ccttttccaa
     1981 tgacatggct actccgatga aggttgtaga accagcaaat agactgccaa gcaagaatcc
     2041 agctaataga gcaataccaa agaaatccca ttcaactgaa cttagaccac cattctccgt
     2101 gaaacggcta aaacttggaa aaagcgcaac tcccatgatg attggttgta tcatggtttg
     2161 gaccatgagg ttgctatctt taagagtaga taggtggtgt ttcactaaag cctgattgag
     2221 attgctagga atcttggcag acttagcatt attggtccgt tttgtcgaag cattttcaat
     2281 cgccaaaact tgattaaagt agttgggaat gacagtttta aacgcaaaca caatcagtcc
     2341 aatcatcaaa atgaaaggaa gtccaaaatg aaggagtgtt tctagtgata tagtatggct
     2401 gaccaagtaa tggaatccag caaatatagg tatgatagga aaatcaacaa agccaccaga
     2461 ttcgagtgaa acggtctgct gggattgcag gaacatgagc gcagcaaaag ccagcaagga
     2521 agaacctgtc atcaggattg tagaaatcaa tgttttacga gggctttttg tcagaacttc
     2581 cccaacaaag tgcatgataa tcagattgac taaattagct aaaatccaga gaatcaaaaa
     2641 ggaaggaatg gaagctaagg cgaggagcgg accgccaatt tgccagtaag tgattagaag
     2701 taaagacaga cacggcatga ggaagggtag gaccatccct tgtgcagaga ggattttagc
     2761 gaggaagact tcttgtggct tcaggggaag tgctaaatat aactttgtat ccttactatc
     2821 gtagaaaacg gagaagaaac ctgtaaaacc gtagacaatg gcaataatgg taaagagcgc
     2881 tagttgcaga gaaaagaaac cgatagactg actataatct actcccatga aaaagacaga
     2941 atagagaagt ccgaaactca aaaccataaa tacttgttgc aaaaggacac ttttataggc
     3001 tgaaaaggag gcgtctttgc gcttactttg tttcttcttg acggaagcta gaagttgagg
     3061 attggaataa agaatattaa tctttactag ttcccaaatg atggatttac tcatacttcc
     3121 tcacctgcct tcacttgttg gcgtccggct aattccaaat agatggtctc caaatcttta
     3181 tctggatact gtgatttgag ttcatcaaga cttcctacga aaatgagttg cccttttcgg
     3241 agaatagcaa tgcggtcaca gagttgttca gctactgaaa ggacgtgtgt agaaaataga
     3301 acggtgtgac cagattgagc atgttctttc atcatttgtt ttaggtcgaa agaagcctga
     3361 ggatccagtc cagttagggg ttcatccaaa atccaaatat cagggttagg gatcaatgca
     3421 ccaataataa tggttttctg acgcataccg tgtgaaaaac tatcaatagg ctcatgctgg
     3481 tgcgctgtca tatcaaatag actagatagt ttagcgatac ggtcatcaac tgtatccttt
     3541 tctacaccat agaccttgcc cataaagtgc cagtattcaa aagctgttaa gtgtaggaag
     3601 atgtctggcg aatctggaac ataggctatc cgttttttga cttcatcgcg gttctcctct
     3661 aggtttaaac catcaacaga gatagttcca taactagctt cgattatcga agtcagtaga
     3721 ctgattgtcg ttgtttttcc tgccccattg tgaccaatta aaccgaaaat ctcgccgctt
     3781 tcaattgtca aatttaggtc atcaagtgcg attgtttcgc cgtatgtttt tgtgatatgg
     3841 tcaaattgta tcataataac ctccttgtta ttcgtgaact aaaagtaggg gcttgtaggt
     3901 attccaagcc ttattccaaa tgttcttcga ataatatata caattaatta catttattat
     3961 actgctgaat tagtacaaaa gcaatagttt ctatcaatat ttttgtagta tggatacgga
     4021 tatatttcaa gaagtcgagt gtaaaaagga atgcaataag ctcgattatc agaaaatttg
     4081 ctataataga cctatgaacg atttgatcaa gcataaattg gagttactac cagatagccc
     4141 tggctgttac ctccataaaa acaagtacgg taagattatc tatgtgggca aggccaaaaa
     4201 cctacgcaat cgggtgcggt cttattttcg tggtgcccat gataccaaga cagaggctct
     4261 agtggctgaa attgccgatt ttgaatttat cgtgacggat tccaacatcg aggccctctt
     4321 gctagaaatc aatctgattc aggaaaacaa gccacgctat aacatcatgc tcaaggatga
     4381 caagtcttat cccttcatca agattaccaa ggaacgtcat ccgcgactaa tcattactcg
     4441 gcagattaag aaggatggtg gtcagtattt tggtccctat ccagatgtgg gtgcggccaa
     4501 ccagaccctc aaactattgg agcgcctcta tcctttccgc aagtgtaaat tgcctgagaa
     4561 caagtattgt ctttattatc atttgcggca atgtttggct catggagaca atatgcccag
     4621 cctgtctgac tatgacaaga tggtcaagga agtggcccag ttcttgacag gtcatgatga
     4681 taagattatc aaggaagtgc aggacaagat gaaggcagct gctggcaatt tggaatttga
     4741 aaaggctgcg gaataccggg atgtcctcca atccatttca accctgcgga ccaagcaacg
     4801 tgtgatggct catgacttgc agaatcgaga tgtctttggc tactacgtag acaagggctg
     4861 gatgtgtgtg caggttttct ttgttcggca ggggaaactg attgagcgga atgtcaatct
     4921 ttttccatac tataatgatg cggacgaaga ctttttgacc tacatcggtc agttttacag
     4981 ggaccagcaa catttgattc cctcagaggt cttgataccg caggatattg accaagaagc
     5041 cgtagaagct ctggtagata ccaaggtcat caagccccag cgtggcgaga agaagcaact
     5101 catcaatctg gcaaccaaga atgcgcgtgt cagtctggag cagaagttta atcttttgga
     5161 gaaaaatctg gagaagacct acggtgcggt ggagaatatc ggtcagcttt tggggattcc
     5221 gacgcctgtg cggattgagg ctttcgataa ctccaatatt atggggacta gtcctgtgtc
     5281 ggctatggtt gtctttgaaa atggggtgcc taataaaaaa gaataccgca agtacaagat
     5341 taatacggtt gaggggccag atgattatgc ctctatgcgt gaagtgctgc aacgccgtta
     5401 tagtcgggtc ttgagggacg atttgacacc gcctgatttg attatcattg acggaggtca
     5461 ggggcaggtc aatgtcgcca agcaggtcat cgagcaggag ttgggtatgt ccattcccat
     5521 tgcaggtttg cagaagaatg acaagcacca aacccacgaa ttgctctttg gcaatccgct
     5581 ggaagtggtg gagttgccac gtaattctca ggaatttttc ctcctgcatc ggattcaaga
     5641 tgaggttcac cgctttgcta ttgagtttca tcgtcaggtt cggtcaaaaa attccttctc
     5701 atcaaaattg gacggcatag aaggcttggg accaaaacgc aagcaggctt tgatgaagca
     5761 tttcaagtcc attggcaata tccagtcagc cagcttacaa gaaatcgcag aagcaggagt
     5821 gccttacaag gtggcggaaa aggtcaagga aagcctttct tcaaaggatg agatttgaaa
     5881 acattcccat aatgtgttag aataaactat caatgactaa ataaggagaa agcaatggct
     5941 tttggtgaag aaaaacacaa aaaaacaaca tttgagaaag tgactttagt aatcgttgtc
     6001 gtgatggttg tcgcgacatt ggctggcttg attttaccag ctattaatgc attgatgaat
     6061 taagaacctg tatttacagt tcggcacttt tatctaaggt tggtttttaa gctgttcatc
     6121 gttagttttt tcaaaataca gtcagtattc tctcaaagaa cgaccttgat tagctaaaaa
     6181 acttttctta tatataagca ctttgcttgt gaatagaggt ctaaaacgct tttaagcgtt
     6241 tttttctagg aaagaatagg taaattatga gtatgttttt agatactgcc aagattaagg
     6301 tcaaggcagg gaaaggtggc gatgggatgg ttgccttccg tcgtgaaaag tatgtaccaa
     6361 acggcggtcc ttggggcggt gatggtggtc atggtggaaa tgttgtcttc gttgtagatg
     6421 aaggcttgcg gaccttgatg gacttccgtt acaatcgtcg ctttaaggct gatgatggtg
     6481 aaaaagggat gacaaaaggt atgcacggtc gaggagcgga agatttaatt gttcgtgtac
     6541 cgcagggaac aactgtgcgt gacgctgata caggtaaaat cattacagac ctggttgaga
     6601 atgggcagga atttgtcatt gcccacggtg gtcgtggtgg tcgtggaaat attcgttttg
     6661 cgacacctaa gaatccagcg cctgagattt ctgaaaatgg agaaccaggt gaggagcgca
     6721 atcttgaatt agaattgaaa gtgttggctg atgtaggtct tgtaggtttc ccatcagttg
     6781 gtaagtcaac gcttctcagt gttattacag cagctaaacc taaaattggt gcctaccatt
     6841 ttacaaccat tgtgccaaat ttgggaatgg ttcggaccaa atctggcgag tcatttgctg
     6901 tggcagactt accaggtttg attgagggtg ctagtcaagg tgttggattg ggaacgcaat
     6961 tccttcgtca cattgagcgg acacgtgtta tcttgcatgt tttggacatg tcagcaagtg
     7021 agggtcgtga tccttatgaa gattacgtag ccatcaacaa tgaattggaa acctacaatc
     7081 ttcgtctcat ggagcgtcct cagattatcg ttgccaacaa aatggatatg ccagaagcgg
     7141 cagaaaatct ggaagagttc aagaaaaaat tggcagccaa ctatgatgag tttgaagaat
     7201 tgccacagat tttcccaatt tctggtattg ctcaccaagg tttgaaaaat cttttggaag
     7261 ccacagctga gttgctagag aaaacgcctg aattcttgct ctacgaggaa tcagatttcc
     7321 aagaagaaga ggcttactat ggctttaacc cagatgagcc agaatttgac atcagccgtg
     7381 cagatgacgc aagctgggtt ctatctggtg acaaactaga aaaactcttc accatgacca
     7441 acttcgatcg cgacgaatct gtcatgaaat ttgctcgtca gttacgtgga atgggtgtcg
     7501 atgaagccct ccgtgcgcgt ggtgccaaag acggcgacat cgtccgtatt ggcaagttcg
     7561 aatttgagtt tgtggattaa cgtagcagtt tgactgcaag ttaacttgct agtcaaacta
     7621 tgccaaatcc tatgggacct tggctagcta ccttactaac ttcacccaaa tggtagtatc
     7681 gaccatttgg gctccgtgtc gtaacgttgg aaacacactt aaatcctaaa ggacttgatg
     7741 tgatacggta gccgctatgg cggactacct accaacctta ctaattccgc acaggcagtg
     7801 gtatcgactg cctgtactcc atatcgtaac ctgtccggta agttagtcta gtatgaactt
     7861 agaaattcga gagcagacta tagctttaca aatttcgttc agttggtgac agtcgtttgt
     7921 gagcaatcga aaaaaaaata gttggaggtc tgtatgggtg ataagccaat atccttcaag
     7981 gataaagatg gaaattttgt ttcagcagcc gatgtttgga atgctgaaaa attagaagaa
     8041 ctctttaaca agcttaatcc caatcgcaaa ctccgtttgg aacgtgagcg tttggcaaga
     8101 gagcaagaaa aggaataacg tcaaaatctg cttgttgtat cagaaaacag gcagattttt
     8161 ttaaaattta
//
LOCUS       DBNFEG010000094         8110 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.094, whole genome shotgun sequence.
ACCESSION   DBNFEG010000094 DBNFEG010000000
VERSION     DBNFEG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8110
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.094"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            41..223
                     /locus_tag="AC1PNG_001358"
     CDS             41..223
                     /locus_tag="AC1PNG_001358"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HIP6701860.1"
                     /translation="MPFVRIDLFEGRTEEQKIALAREVTEVVSRNTNAPKEAIHVFIN
                     DMPEGTYYPQGEMKRK"
     gene            complement(273..2105)
                     /gene="lepA"
                     /locus_tag="AC1PNG_001359"
     CDS             complement(273..2105)
                     /gene="lepA"
                     /locus_tag="AC1PNG_001359"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIP6701861.1"
                     /translation="MNLEDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPIINKIDLPAADPERVRQEIEDVI
                     GLDASEAVPTSAKAGIGIEEILEQIVEKVPAPTGDVEAPLQALIFDSVYDPYRGVILQ
                     VRIVNGVVKPGDTIQMMSNGKTFDVTEVGIFTPKAIGRDYLATGDVGYIAASIKTVAD
                     TRVGDTVTLAKNPASEPLAGYKQMNPMVFAGIYPIDSNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERIEREFNIDLIMTAPSVVYHVNMTDGEMID
                     VANPSEFPDPTKIATIEEPYVKAQIMVPQEYVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYEISEYRSSKLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKIIVDKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDEDDKK"
     gene            complement(2201..2614)
                     /gene="ndk"
                     /locus_tag="AC1PNG_001360"
     CDS             complement(2201..2614)
                     /gene="ndk"
                     /locus_tag="AC1PNG_001360"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIP6701862.1"
                     /translation="MERTFFIIKPDALKRGLAGQILSRIERRGFQIRDLKMVTATEEL
                     ISQHYDHLTDKPFFPQLVQYMTSGPMIAGIIEGPEVIKSWRDMMGATNPVNALPGTIR
                     GDFATAPVEGIVANVVHGSDSAEAAEREIGLWLGK"
     gene            complement(2637..3224)
                     /gene="yihA"
                     /locus_tag="AC1PNG_001361"
     CDS             complement(2637..3224)
                     /gene="yihA"
                     /locus_tag="AC1PNG_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIP6701863.1"
                     /translation="MEINTHNAEILLSAVSKAQYPGDDIPEIALAGRSNVGKSSFINT
                     LLGRKNLARTSGKPGKTQQLNFYNIDDKIRFVDVPGYGYAKVSKTERAKWGKMIEEYL
                     TTRENLRVVVSLVDMRHEPSADDVQMYDFLKYYEIPVIIVATKADKIPQGKWNKHESM
                     IKKKLDFDKNDQFVIFSSETRHGYDQAWDAILSNI"
     gene            complement(3280..4506)
                     /gene="clpX"
                     /locus_tag="AC1PNG_001362"
     CDS             complement(3280..4506)
                     /gene="clpX"
                     /locus_tag="AC1PNG_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIP6701864.1"
                     /translation="MAVKHTHELIYCSFCGKNQEEVKKIIAGNNVFICNECVELAQEI
                     IREELAEEVLTDLADTPKPQELLNILNNYVIGQDRAKRALAVAVYNHYKRINFQDSRD
                     ENDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVENIL
                     LKLLQAADFNIDRAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTVA
                     SVPPQGGRKHPNQEMIHVDTKNILFIVGGAFDGIEEIVKQRLGEKIIGFGHNNRAIDE
                     KESYMQHIIADDIQKFGIIPELIGRLPVFAALDQLTTEDLVRILTEPKNALVKQYQTL
                     LSYDDVELDFDEDALLAIAEKAIERKTGARGLRSIIEETMLDVMFEVPGQDNVKRVRV
                     TKAAVDGKEKPLLETA"
     gene            complement(4704..5216)
                     /locus_tag="AC1PNG_001363"
     CDS             complement(4704..5216)
                     /locus_tag="AC1PNG_001363"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIP6701865.1"
                     /translation="MTKKIVAIWAQDENGLIGKGDRLPWSLPADLAHFKETTIGHTMV
                     MGRITFDGMGKRALPNRHTIVLTTDKTYQLESERITILHSVEDVLDWYNKQEQTLFVI
                     GGGQIFTAFAPYIETLIVTDIHGQFNGDVYFPKAFPMEKFQLQSANLRPKDEKNPYDF
                     TIKTYERRDG"
     gene            complement(5554..6393)
                     /locus_tag="AC1PNG_001364"
     CDS             complement(5554..6393)
                     /locus_tag="AC1PNG_001364"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIP6701866.1"
                     /translation="MTKADIIFKENIRKIMEEGVFSENARPRYKDGNVANSKYVTGAF
                     AEYDLSKGEFPITTLRPIPIKSAIKEILWIYQDQTNDLSVLQEKYGVQYWNDWEVEGT
                     GTIGERYGAIVKKHDIISKILKQLEENPWNRRNVISLWDYEAFDQSAGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAEELLRRTPSEVEPRLVLNVPDGTNFFDIRAEDFELVDYNPVKPQLKFD
                     LAI"
     gene            complement(6446..6748)
                     /locus_tag="AC1PNG_001365"
     CDS             complement(6446..6748)
                     /locus_tag="AC1PNG_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701867.1"
                     /translation="MNLTLLKRLNLVLYGIAIFLFVMLFLPIGQWFDIVNVNFKLTFF
                     IIPFFGLASLPTAIYTKNVRQILLSAILVALYFILFSLITALSGLFHLNFYSFFFK"
     gene            complement(6760..7038)
                     /locus_tag="AC1PNG_001366"
     CDS             complement(6760..7038)
                     /locus_tag="AC1PNG_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701868.1"
                     /translation="MKQVNMSKIINYLTILGLLILLSAFFLDNWIRDWFFPSSWGNVA
                     TMLILPLLGTLILILSIYYKKLWTGLISIFLMISFPLIFGIGYFIFGP"
     gene            complement(7165..8109)
                     /locus_tag="AC1PNG_001367"
     CDS             complement(7165..8109)
                     /locus_tag="AC1PNG_001367"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HIP6701869.1"
                     /translation="MGIDLGGTSVKLAILTTEGEIQEKWSIKTNILDDGSHIVPDIID
                     SIQHRFETHGLTKDNFLGIGMGSPGVVDSEAGTVIGAYNLNWKTLQLVKDQFESALGL
                     PFFIDNDANVAALGEQWVGAGNNNPNVVFMTLGTGVGGGVIAAGNLIRGVKGAGGELG
                     HITVDFDEPFACTCGKKGCLETVASATGIVNLSRRYADQYAGDAKLKQMIDDGQDVTA
                     KDVFDLAKEGDDLALIVYRHFSEYLGVACANIAAVLNPAYIVLGGGVSAAGELLLDGV
                     RKVFAENSFPQIKESTQIVLATRGNDAGVLGAASLVLK"
BASE COUNT         2367 a         1797 c         1547 g         2399 t
ORIGIN      
        1 aaactatggt acaataaacg aaaaataaag gaggaaatat atgccatttg tacgtattga
       61 tttgtttgaa ggacgcacag aagaacaaaa gattgcctta gcacgtgaag ttaccgaagt
      121 tgtatctcgt aatactaatg cgcccaaaga agccattcat gtcttcatca atgatatgcc
      181 tgaagggacc tactacccac aaggtgaaat gaaacgaaaa taagagaaaa cactcttgca
      241 agaaaatgca ggagtgtttc tttgtatcaa gtttatttct tatcgtcttc atccatgctg
      301 agaacactga ggaaggcttc ttgtggaact tcgacagagc cgatggcttt catccgtttt
      361 ttaccagctt tttgcttttc taggagtttg cgtttacggg aaacgtcacc accataacac
      421 ttggccaaaa cgttcttccg aagagccttg atgtcgctac gggcaacaat tttctgaccg
      481 atagctgctt ggattggtac ttcaaactgt tgacgaggaa tgattttttt gagtttgtcc
      541 acgataattt tcccacgttc gtaggcaaat tccttgtgga cgataaagct gagggcatcg
      601 accttatcac cattgaggag aatatccatt ttcaccaact tggatgaacg gtattcagaa
      661 atctcatagt cgaagctggc ataaccacga gtggacgact taagcttgtc aaaaaagtca
      721 aaaacaatct cagccagtgg aatttgataa ataacattga cacgattatc atcgatgtaa
      781 tccatagtca caaagtcacc gcgtttgcgt tgggccaatt ccatgactgc accgacgtat
      841 tcctgtggta ccatgatttg tgccttaacg taaggctctt cgatagtggc aatcttggtt
      901 gggtctggga attcggatgg gttggctaca tcaatcattt caccgtccgt catgttgaca
      961 tggtagacaa ccgacggtgc tgtcatgatg aggtcaatgt taaactcacg ctcaatccgt
     1021 tcttggataa catccatgtg caagaggccc aagaatccac aacggaaacc aaatccgagt
     1081 gcctgcgatg tttctggctc aaactgtaag ctggcatcgt tgagttggag tttttccaag
     1141 gcttcacgga ggtcattgta cttgttggaa tcgattggat agatacctgc aaagaccatt
     1201 ggattcatct gcttgtagcc cgctagtggc tcgctagcag gattttttgc caaagttacc
     1261 gtatcaccga cacgggtgtc ggcaaccgtc ttgatagagg ctgcaatgta acctacatca
     1321 ccagtcgcca aataatcacg accgattgct ttgggtgtga agatacccac ttccgtcaca
     1381 tcaaaggttt tgccattgct catcatctga atagtatcgc caggttttac cacaccgttg
     1441 acaatacgaa cttggaggat aacgcctcga tacggatcgt aaaccgagtc aaaaatcaag
     1501 gcttgcaatg gagcttcgac atcaccagtc ggtgctggaa ccttctccac gatctgctcc
     1561 aagatttcct cgataccaat acctgccttg gctgatgttg gcactgcttc ggaagcatcc
     1621 aaaccaatca catcttcgat ttcctgacga acacgttctg ggtctgctgc tggtaggtca
     1681 atcttgttaa tgattggcaa gatttccaaa tcattgtcca aggctagata aacgttagcc
     1741 aaggtctgtg cttcaatccc ttgagcagca tccaccacta aaatcgctcc ttcacaggca
     1801 gccaacgaac gcgatacctc ataggtaaaa tccacgtgtc ctggtgtgtc aatcaagtgg
     1861 aaaatgtatg tctctccatc tttagctgta tagttgagtt cgattgcgtt gagtttaatt
     1921 gtgataccac gctcgcgttc caagtccata ctatctagca actgggcttg catttcacgg
     1981 ctggaaactg tttctgtctt ctcaagaata cggtcagcca aggttgattt accatggtcg
     2041 atgtgggcaa taatggagaa attacgaatt ttctcctgtc gttttttcaa atcttctaaa
     2101 ttcatgtttc ttcctctaca aggctattac tcctcattat atcagattta gtgataattt
     2161 tccataagaa aaacacctag aaaactaggt gtctgctctg ctattttccc agccaaagac
     2221 cgatctctcg ttcggctgct tctgcactat ctgagccatg aacgacattt gccacaattc
     2281 cctcaacggg cgctgtcgca aaatctccac gaatagtccc aggtagggcg tttactggat
     2341 tggtagctcc catcatatct cgccaagatt tgataacttc tggcccttct ataatgccag
     2401 ctatcatcgg cccgctcgtc atatattgta ctaattgcgg gaagaaaggt ttatctgtca
     2461 aatgatcata atgttgactg attagctctt ctgtcgctgt aaccattttt aaatctcgga
     2521 tttgaaatcc cctgcgttcg atacgtgaaa gaatttgtcc tgcaagtcca cgcttaagag
     2581 catctggttt gatgatgaag aaagtacgtt ccatgatttt tctcttgaac tgtttattag
     2641 atgttactta agatagcatc ccatgcctga tcgtatccgt gacgagtctc agaagaaaag
     2701 ataacgaatt gatcattttt atcaaaatcc aatttctttt taatcatcga ttcgtgctta
     2761 ttccactttc cttgtgggat tttatcagcc ttcgttgcca caataataac aggaatttca
     2821 taatatttca agaaatcata catttgaaca tcatctgctg atggctcatg acgcatgtct
     2881 actaaactca ccacaacacg tagattttct cgtgtcgtca aatactcttc aatcattttg
     2941 ccccacttag cgcgctccgt tttggatact ttagcatagc cataacctgg aacatctaca
     3001 aatcgaattt tatcatctat attataaaaa ttgagttgtt gagtctttcc tggttttcct
     3061 gacgtacgtg caagattttt tcttcctaga agagtattga taaatgagga ctttccaacg
     3121 ttagatcgcc cggcaagggc aatttctgga atgtcatcgc ccggatactg ggcttttgaa
     3181 acagcactga gcaaaatctc agcattatgt gtgttgattt ccattttata cctttcataa
     3241 ataagggtag aaaacaatca gtttccaccc tttatttttt tatgcagttt ctagtaaagg
     3301 cttttcctta ccgtctactg ctgctttggt tacacgaacg cgtttaacgt tatcttgacc
     3361 tggcacctca aacatgacat ccaacatcgt ttcttcaatg atggagcgaa gtccacgagc
     3421 accagtttta cgttcaattg ctttttctgc aattgctaac aaggcgtcct catcaaagtc
     3481 aagctccacg tcatcataag acaagagtgt ctggtattgt tttacaagag cattcttcgg
     3541 ttccgtcaaa atgcgtacca aatcctctgt tgtcaattgg tccaaagctg cgaatacagg
     3601 caagcgacca atcaactctg gaatgatacc gaatttttga atatcgtctg caatgatatg
     3661 ttgcatgtat gattcttttt catcaatagc acgattatta tgaccaaaac cgatgatttt
     3721 ttcaccgaga cgctgcttga cgatttcttc gataccatca aatgcaccgc caacgataaa
     3781 caggatattt ttggtgtcca cgtgaatcat ttcttggttt ggatgtttgc gtccaccttg
     3841 tggtggtacg ctggccacag ttccctcgat aatttttagg agagcttgct ggacaccttc
     3901 accagataca tcgcgggtaa tggacacatt ttcacctttt ttggcaattt tatcaatttc
     3961 atccacatag ataattcctc tttctgcacg gtcaatgtta aagtctgcag cctgcaaaag
     4021 tttgaggagg atattttcta cgtcttcacc aacataaccc gcctcagtaa gagcggtcgc
     4081 atcggcaata gcaaacggga cattcaaact tttagccaag gtctgagcta ggaaggtttt
     4141 tcctgaacca gtcgggccaa tcataaggat atttgatttt tgcaaatcaa catcgttctc
     4201 atcacggcta tcttggaaat tgatacgttt gtagtggttg taaacggcta ctgctaaagc
     4261 acgttttgca cgatcctgtc cgatgacgta gttgttcaga atattgagta attcttgtgg
     4321 ctttggcgta tcggccaagt ctgtcaaaac ttcttctgcc agttcctcac gaatgatttc
     4381 ctgtgctagt tccacgcact cattacaaat gaagacattg tttcctgcga taattttttt
     4441 aacttcttct tgattttttc cgcaaaatga gcaatagatt agctcatgtg tatgcttaac
     4501 agccatgtgt ttcctctttc atatctctta tctaaataaa aatccataaa tggcgtgaat
     4561 actattgacc agattggtaa aagcattcag taaaaataaa attgctaaac caaaacgttt
     4621 cttccgaaag gcttgaatcg cacaaagaac acaaatccca cagagaaatg ctgtaaataa
     4681 accgaataaa cttctctcca tgcctaacca tctcttcttt cataggtttt tattgtaaaa
     4741 tcatacgggt ttttctcgtc ttttggacgt aaattggcgc tttgtagctg aaatttctcc
     4801 ataggaaatg ctttcggaaa atacacatcg ccattaaatt gaccgtgaat atctgtcact
     4861 attaatgttt caatgtaagg tgcaaaagca gtaaaaattt gtcccccacc aatcacaaat
     4921 agtgtctgct cctgtttgtt ataccaatct agaacatctt ccacactgtg taaaatggta
     4981 atacgttcac tctctagttg ataggtttta tccgttgtca aaacgatggt atgacgattg
     5041 ggaagagcac gtttacccat gccgtcgaaa gttatacgtc ccatcaccat cgtgtgacct
     5101 attgtcgttt ctttgaaatg agctaagtca gctggcaatg accaaggaag tctatcacct
     5161 tttccaatca atccattttc atcttgagcc caaatggcaa caatcttttt agtcattttc
     5221 tctcactttc cgtcctatca ttctatcaaa aaaacatgaa aaatgcactt accaagtagg
     5281 ccaagcgcaa atttttatca tgttgaaact atcaccaact cctcagagat agggaggagt
     5341 ccgagattcg tagaatgtga acctcgctgg tgctggaaag tccccctcat cttaaatcgc
     5401 taagtcaaaa ttaagttgcg gtttaactgg cttcgaaagt ctgagtatag actgttgaag
     5461 gttggaagtg aaacagttcg gggaactgtt tcagcccgag cctaaaaata aagaagcgag
     5521 gtaaaagaac gaagttcttc actgctaatc cagttaaatt gctaaatcga attttaactg
     5581 cggtttaact ggattgtagt ccaccaactc aaaatcttct gcccgaatgt caaagaaatt
     5641 ggtgccgtca ggaacattta agaccaaacg tggctcaact tcagatggtg tgcgacggag
     5701 taattcttca gcctgttcaa actgattatc atagatgtga agattgttga tgaaatagaa
     5761 aaacttccca accttccagc caaaatgttt agcaatcatc atctgaaggg ctacatactg
     5821 catagcgttg atatgatggg ctaccaacat atcatttgac cgttgagtta gagttgcatc
     5881 caaataaatc tccccatcca ctcgccgaac gtcaaacatg gtctgaaagg cacatggcaa
     5941 aagacccgcg gattggtcaa atgcttcata atcccacaaa gaaatcacat tacggcggtt
     6001 ccatggattt tcctccaatt gcttgaggat ttttgaaatg atgtcatgct ttttaacaat
     6061 tgcaccatag cgttcaccga ttgtgcctgt tccttcaact tcccagtcat tccaatactg
     6121 gacaccgtat ttttcctgta aaaccgacaa atcattggtc tggtcttggt aaatccagag
     6181 aatttccttg atggctgatt taatcggaat aggtctaagt gttgtaatag gaaactcccc
     6241 cttagacaag tcatactcag caaaggcgcc tgtaacgtac ttggaattag cgacatttcc
     6301 atccttgtaa cgtgggcgag cattttctga aaaaacgccc tcttccataa tcttacgaat
     6361 gttttcttta aaaataatat ctgctttagt catggtctta gtatagcaaa ttctagcaaa
     6421 aaactccagt tttgtaccgg agttctcatt tgaaaaagaa tgaataaaaa ttaaggtgga
     6481 acaaaccaga tagggcagtt attaggctga acagtataaa atacaaggca accaaaatag
     6541 cactcaacaa tatctgacga acatttttag tataaatagc cgttggtaag ctggctagtc
     6601 cgaaaaatgg aatgatgaaa aatgttagtt taaaattcac atttacaata tcaaaccatt
     6661 gccctattgg caaaaatagc atgacgaaga gaaaaattgc gataccatac aaaactagat
     6721 ttaatctttt tagtaaagtc aggttcataa ccgctccttc taggggccaa atataaagta
     6781 gccaataccg aatatcagag gaaatgagat cattaagaag atactaatca aaccagtcca
     6841 caatttttta taatagatag atagtataag aattagagtc cccagtagag ggagaatcaa
     6901 catggttgct acgtttcccc agctagacgg gaaaaaccag tcacgaatcc agttatcaag
     6961 gaaaaaagca gataacaaaa tcaataaacc aagaatggtc agatagttga ttattttaga
     7021 catgttcacc tgtttcataa ggtgacactc ctttctcttt acactttcat tgtacgctta
     7081 aatactacat ttttcaatat atgtatataa agaaaatgtg acagcggaat tgaaaaaaag
     7141 cagagtttcc cctgctttta acgattattt caagacaagt gatgctgcgc ccaatacacc
     7201 tgcatcatta ccacgtgttg ccaagacaat ttgcgtgctt tccttgattt gtgggaagct
     7261 attttcagca aagactttac gaacaccatc caataagagc tctcctgcag ctgatacacc
     7321 gccacccaga acgatgtagg caggattgag gactgcagca atgtttgcac aagcaacacc
     7381 taggtattct gagaagtgac ggtagacaat caaagccaaa tcatcgcctt cttttgccaa
     7441 atcaaagaca tctttagccg ttacatcttg cccatcatca atcatttgtt tcagttttgc
     7501 atcgcctgcg tattggtccg cataacgacg gctaaggttg acaatacctg tcgctgaagc
     7561 cacggtttcc aagcaacctt tcttaccaca agtacatgca aatggctcat caaagtcaac
     7621 tgtgatatgg cccaattcac caccagcgcc tttgacccca cgaatcaagt ttccagcagc
     7681 gatgacaccg ccaccgacac ctgtaccaag cgtcataaag acaacgtttg gattgttgtt
     7741 tccagcacca acccactgct caccgagagc cgctacgttt gcatcattgt cgatgaaaaa
     7801 tggtaaaccg agcgcagatt caaactgatc tttaaccaat tgcaaggtct tccagttgag
     7861 gttataggct ccgataacag tgccagcttc actatccact acaccgggtg agcccatacc
     7921 aattcctaag aaattgtcct ttgtcaaacc atgggtttca aaacgatgtt gaatgctgtc
     7981 aataatatct ggaacaatat ggcttccatc gtctaatata tttgtcttga tagaccattt
     8041 ttcttgaatt tctccctcag tagtcaaaat ggccaattta acagaagttc ccccaaggtc
     8101 aataccgata
//
LOCUS       DBNFEG010000095         8099 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.095, whole genome shotgun sequence.
ACCESSION   DBNFEG010000095 DBNFEG010000000
VERSION     DBNFEG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.095"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..395
                     /locus_tag="AC1PNG_001368"
     CDS             <1..395
                     /locus_tag="AC1PNG_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HIP6701870.1"
                     /translation="EETLAQGILIGIWGTTLLFSFIWYILVAISNYILFKKAGYAGWK
                     SLIPIYNLYIQQCIAFGYEKRWFILFLLIPLAGPLYGIYLVYNFGRSFGLSAVQAIFY
                     VLLTPILNLYIAFNDGSRYQGPQEFFID"
     gene            complement(431..1345)
                     /locus_tag="AC1PNG_001369"
     CDS             complement(431..1345)
                     /locus_tag="AC1PNG_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIP6701871.1"
                     /translation="MNELELLARALGFIFVIGLGYTLKVKGIVRREDARIFSSLVMNI
                     TLPASLLVASATAQVSIDLYVPFILGILCNLFLDMVGYWEAKKSGQQTSVGLMQLSGY
                     NIGTFTLPFVQAFFPLSSLLSVIVFDAGNAIMVLGGNYSIAAGVDEEQERMTVATFIK
                     NLSRSVPLVVYLVSLSLAVCSIKLPRELISALTIASNANPFLAMLMLGILLDLKLNWQ
                     EISRLVYLLSLRLGANILIAGVIYFLLPIDKTMKIMLLVCLVSPISVMSPIYALKLGS
                     RSAEPANVNSLSILVSLVMMVLLIFLFV"
     gene            1476..1793
                     /locus_tag="AC1PNG_001370"
     CDS             1476..1793
                     /locus_tag="AC1PNG_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIP6701872.1"
                     /translation="MYYPVSSILIEFLVLAIVEKQDSYGYEISQTIKIVADIKESTLY
                     PILKKLEKAGYVTTYTQEFQGRKRKYYTITPAGREHLPFLQGEWNSYKDHIDGIIEGS
                     LRK"
     gene            1790..2902
                     /locus_tag="AC1PNG_001371"
     CDS             1790..2902
                     /locus_tag="AC1PNG_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIP6701873.1"
                     /translation="MTRADYLNQLEQALFQLHPSARQEALDYFNEYFDEKDNDEEAII
                     ELGTPDEAAKEIIANLPEDALIPEKDQASPHSSKPFDFNFDFDFQFDWENFFNNFKHS
                     AYQARERIKLTSKIEELPEFSNLQISLEDQALSVQSYEGEAVLLHNPVSEDGSYQAFD
                     YQLVQDSLYISSKPTPNHLYFSNNQISSAILKIPKKLLPLTNLNLKTADSSLTIVDVQ
                     VCEQMVVNAKDSSISMTKVSCPSSALQLEDSTLTISDGQMSELKLEASDAVITLSSMS
                     LSDARITLEDCVWKLKDFVLEKSLNCQAEDSVLTYKPSTDISLDIWEEDSSLKLPKDK
                     AFTMMKEGDITHLSYKQENSPAQLVIHCQDCQLSVG"
     gene            2914..3492
                     /locus_tag="AC1PNG_001372"
     CDS             2914..3492
                     /locus_tag="AC1PNG_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HIP6701874.1"
                     /translation="MNRKEYLQNLEKYLKNLSKEDFQDTLDYFNEYFDEKDNDEEAII
                     ELGTPDEAAKEIIANLYDKERIEDKPNTSNMVWLTILAILAAPIGVPLAITLVALFIT
                     LLLIIFSLFLLLISLWIVLLSLAIAQLLLAFDLLTLSWSSSLLFTGLALVCLALTLLG
                     SKFTLDLGSKTFLLVLHWVQRKIRKGGYYETA"
     gene            3479..3940
                     /locus_tag="AC1PNG_001373"
     CDS             3479..3940
                     /locus_tag="AC1PNG_001373"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701875.1"
                     /translation="MKQLKPFTKISLSLLGLGAILSLIGYLAGGWTNMKTVAENYFQP
                     QTASFTDIRSIEVNEKLSIMPSPDNQFHLHYYRNQHKDIDLLSHQVQDGKLTISSAYQ
                     GIVTYDGLLEPILNLVNDQNVAYYQPVLQVPKIAQSKHSQGAFKEMSIWIK"
     gene            3937..4332
                     /locus_tag="AC1PNG_001374"
     CDS             3937..4332
                     /locus_tag="AC1PNG_001374"
                     /inference="COORDINATES: protein motif:HMM:NF024743.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIP6701876.1"
                     /translation="MTIQDFQLFVENGDMLIENSHISLIRLLHINGEVAINQSTLTSP
                     ADITDTTRSILTVEDGDFKAENLKLEGKHGISNYDGSIDISLHPKTKTDIHIDASQNN
                     LGIDLPTYSNPQATNYLYISTLDGELNIQ"
     gene            complement(4368..4727)
                     /locus_tag="AC1PNG_001375"
     CDS             complement(4368..4727)
                     /locus_tag="AC1PNG_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HIP6701877.1"
                     /translation="MSIFTTILASIVAIEHLYIMYLETFATHSDSTSRVFNMEKEELQ
                     RKSVTTLFKNQGIYNGLLAVFLIYGIFTGNLEVTTIFVLFVIGAAGYGAATSSPKIIL
                     TQGGPAILALISILLFK"
     gene            complement(4747..5172)
                     /locus_tag="AC1PNG_001376"
     CDS             complement(4747..5172)
                     /locus_tag="AC1PNG_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIP6701878.1"
                     /translation="MQEMEDLLYRLKVADETISNLFEKQLGISLTRYSILQTLLKDAP
                     LHQLALQERLQIDRAAVTRHLKLLEESGYIIRKRNPDNQREVLVWPTEQAREALITNP
                     SAHHQAIKTSMNQILTVEESEQFLATLDKLLIGLQNLPI"
     gene            5333..6091
                     /locus_tag="AC1PNG_001377"
     CDS             5333..6091
                     /locus_tag="AC1PNG_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIP6701879.1"
                     /translation="MENTPRLNLVIDVLMLAGAILAQSGAEIHRVEDTMIRIAHSHGI
                     EQANVLAIPAAIFFSIDHTNISRMKRIVNSNYDMQKVCDVNQISRSLANEEISLEEAW
                     SQLNAIKQQKPPYNNIQITLAAVVAAPFFTIMFGGSLLDAAGAVLATALAFPISLIVE
                     RYIGIDFVTTFVGAFVFSLTSYLLSATTTLPINPNLINAGAVMPFVPGIAFTNAVRDL
                     MTNHINTGMTKFFQTALIILSLGAGTTVAFMLVR"
     gene            6095..6547
                     /locus_tag="AC1PNG_001378"
     CDS             6095..6547
                     /locus_tag="AC1PNG_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIP6701880.1"
                     /translation="MTPVHFLLQALASYIAIAAFLIVLNVQRKMLVPGGLLGMLVWLI
                     YLLLLEPTNVLIATFFAAIIGSCVSQIMSIWLKTPSVIFSLAILAPLVPGYRAYMTTT
                     YFVSGDHAQALTNITTVLTLALVIPIGMASGTILLRLYKVLRTGKKAA"
     gene            6604..7842
                     /locus_tag="AC1PNG_001379"
     CDS             6604..7842
                     /locus_tag="AC1PNG_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIP6701881.1"
                     /translation="MNQNVTNLKLAERGALLSIGAYIVLSGIKLVAGQLFHSDALRAD
                     AFNNISDIIGNIAVLIGLKMAQKPADTDHKFGHWKMEDLASLITSFIMFVVGFQVLYD
                     TLQKLISNSSVEVDIMGAIVGIFSALVMLAVYLYNNRLAKKVRSKALEAAAKDNLSDA
                     VTSIGTSIAIFAAAFNLPIVDKIAAIIITFFILKTAYDIFMESFFTLSDGFDENLLKK
                     YEEDILKLPKIVSVKSQRGRTYGANIYLDVVLEMNPDLSVYESHEVTEQVEQLLTLKH
                     GVFDVDIHVEPSEIPHDEMYEHVYDKLFRFETEIQAHANGYEELIDDQYLLIDAKGHY
                     RNKTQILADHPIQTTYLSNYQMTSISHKSKLVTFEIGEYVHTSLWRRHENWTVIFHQI
                     SKKQASQIEATEKISDLHNP"
BASE COUNT         2534 a         1762 c         1459 g         2344 t
ORIGIN      
        1 cagaagaaac attagcacag ggaattctca ttggcatttg gggtacaaca ctactatttt
       61 catttatttg gtatatcctt gttgctattt ccaactacat cctcttcaaa aaagcaggct
      121 acgctggttg gaaatcattg attccaatct acaatctcta tatccaacaa tgcatcgcct
      181 ttggctatga aaaaagatgg ttcattctat tcttactgat tccgctagca ggaccattat
      241 atggcatcta tctcgtttac aattttggtc gtagcttcgg tttatcagct gtacaagcta
      301 tcttctatgt tcttcttaca cctatcttga acctctacat cgcctttaat gatggaagtc
      361 gttaccaagg tccacaagaa ttctttattg attaatatac aatcaagccc ccgcacacgc
      421 ggaggctttt ttaaacgaat agaaaaataa gaagaaccat catgactagg ctaaccaaaa
      481 tagataagga gttaacattg gcaggttcag ctgagcgact ccctaacttt aatgcataaa
      541 ttggggacat gactgaaatc ggagagacta gacagaccag caacatgatt ttcatcgttt
      601 tatcaatagg tagaagaaaa taaatgactc ctgctattag aatattcgca cccagcctca
      661 aagagagaag atagaccaat cgactaattt cttgccaatt caattttaaa tcaagcaaga
      721 ttcctaacat gagcatggct aaaaaaggat tggcgttact ggctatcgtc agcgcagaga
      781 taagttcgcg tggtagtttg atagagcaga ctgctaacga taggctaact agatagacta
      841 ctagtggaac tgagcgtgaa agattcttta taaaagttgc aacggtcatt ctttcctgct
      901 cttcatccac accagctgcg atactgtaat tcccacctag aaccatgatt gcattccccg
      961 catcaaatac gatgacgctt agtagactag atagcggaaa aaaagcttgc acaaatggga
     1021 gtgtaaaggt tccgatatta tagcccgata actgcataag cccgacggaa gtctgttgac
     1081 cagacttctt tgcttcccag tatcccacca tatccaaaaa aagattacaa agaatcccta
     1141 atatgaaggg aacgtataaa tctatagata cttgggctgt cgctgaagct acaaggagag
     1201 aagctggtag cgtaatattc atcactaagc tagaaaagat tcttgcatct tcccttcgaa
     1261 caatcccttt gacttttaat gtataaccta aaccgataac aaagataaaa ccgagagcac
     1321 gggctaataa ttcaagttca ttcatgaaac tagtatagca tatagaaaag aattttgatg
     1381 atatataaaa attttatata atcgcttgat tttgctaaac aataggtata taatttatat
     1441 ataaaataat tacataaaga taaagaggtg agtttatgta ctaccccgta tcctctatct
     1501 tgatagaatt tctggtatta gccatcgtgg aaaagcagga ttcctacggc tatgaaatca
     1561 gccaaaccat caagatagtt gctgatataa aggaatccac actctaccct attttgaaaa
     1621 aattggaaaa ggctggctat gtcaccacct atacccaaga atttcaaggg agaaagcgga
     1681 aatactatac catcactcct gctggacgtg aacacctccc cttcctgcag ggtgaatgga
     1741 atagctacaa agaccatatt gacggaatta tagaagggag cttacgaaaa tgacacgcgc
     1801 agattatttg aaccaactgg aacaagcatt atttcaactt catccaagtg ccagacagga
     1861 ggctttagac tactttaatg agtattttga cgaaaaggat aacgacgagg aagctattat
     1921 agagctagga acacctgatg aagcagcaaa agaaatcatt gccaatctac ctgaagatgc
     1981 cctaatcccc gagaaagacc aagcaagtcc acattcatct aaaccatttg atttcaactt
     2041 tgattttgat tttcaatttg actgggagaa ttttttcaac aatttcaaac attcagccta
     2101 tcaagctagg gaaagaatta aattgacgtc gaaaatcgaa gaattacctg agttttcaaa
     2161 tctacagatt tcgctcgaag atcaggccct tagtgttcaa agttatgaag gagaagctgt
     2221 cttattgcac aatcctgttt cagaagatgg tagctaccag gcttttgact atcaactagt
     2281 ccaagatagt ttgtacatat ctagcaagcc tacccctaat cacctctact tctcaaacaa
     2341 tcaaatcagc tctgctattc tcaaaatccc taaaaaacta ctgcccctta ccaatctcaa
     2401 tctgaaaacg gcagatagta gtctgacaat agtggacgta caagtatgcg aacagatggt
     2461 cgttaatgca aaagatagca gtatcagcat gacgaaagtg agctgcccaa gctctgctct
     2521 acagttagaa gactctacac taactatctc agatggacag atgagcgagt taaaattgga
     2581 agccagcgat gctgttatca ctctctcaag catgagcctt tcagatgcaa ggataacatt
     2641 agaagactgt gtctggaagt tgaaggattt tgtccttgaa aagagcctta actgccaggc
     2701 tgaagattct gtcttaacct ataaaccaag tacggacatc agtttggaca tctgggagga
     2761 agattctagc cttaaacttc caaaggataa ggcgtttaca atgatgaaag agggtgacat
     2821 cactcatctc agctacaagc aagaaaatag ccctgctcag ctagttattc attgtcaaga
     2881 ttgccagctt agtgtgggat aaaggaggtt tccatgaatc gcaaggaata tttacagaat
     2941 ctcgagaaat atctcaaaaa cctatcaaaa gaagattttc aagatacctt ggactatttc
     3001 aatgaatatt ttgacgaaaa ggataacgac gaggaagcta ttatagagct aggaacacct
     3061 gatgaagcag caaaggaaat cattgccaat ctctatgata aagagaggat tgaagataag
     3121 ccaaatacaa gcaatatggt ctggttgacc atcctcgcta tccttgccgc cccaatcggc
     3181 gtccccctag caatcacctt ggtcgccctc ttcatcaccc ttctcctaat catctttagc
     3241 ctctttctgc tccttatctc actctggatt gtcctcctca gcctcgcaat cgcccaactt
     3301 ctcctcgcct tcgacctcct cacactctca tggtcctcct ccctcctctt cacaggcctg
     3361 gccctcgtct gcttggccct cactctattg ggtagtaaat tcactctaga ccttggaagc
     3421 aaaaccttct tgcttgtttt acattgggta caaagaaaga tccgcaaagg aggctattat
     3481 gaaacagctt aaaccattta cgaaaatcag tctgagcctg ctaggtcttg gagccattct
     3541 aagcctcatt ggctacctgg ctggcggttg gacaaatatg aaaactgttg cagaaaacta
     3601 ttttcagccc caaacggcaa gctttacgga tattcgttct atagaggtca atgaaaaact
     3661 gtccattatg ccctcgcctg acaaccaatt ccacctccac tactatcgaa atcagcataa
     3721 agacattgac ctcctcagtc accaagtcca agatggaaaa ttaaccattt ctagtgccta
     3781 tcaaggcatc gttacttatg atggtttact cgaacctatc ttaaatcttg tcaatgacca
     3841 gaatgttgcc tactaccaac ctgtcttaca ggtccctaaa atagcacaat cgaaacactc
     3901 tcagggagca ttcaaggaga tgtctatctg gataaagtga ctattcaaga tttccaactc
     3961 tttgttgaaa atggtgacat gttgattgaa aacagtcaca tcagccttat taggctatta
     4021 cacatcaatg gagaagtagc aatcaatcag tccacattaa caagtccagc ggacatcacc
     4081 gatactacca gaagtattct aactgttgag gatggtgatt ttaaagcaga aaacctaaaa
     4141 ctagagggaa agcatggcat cagtaattac gatggttcaa tcgacatttc tcttcatccc
     4201 aaaacaaaaa ctgacatcca tatagatgcc agtcaaaata atctgggaat tgaccttcca
     4261 acctattcca atccccaagc cactaattac ctctatatct ctacactaga tggagaactg
     4321 aatattcagt aattgcaaaa agccctcctc acggaaggct ttttaactta tttaaacaac
     4381 aaaatagaaa tcaaggccaa gatagctggt ccaccttggg ttaagatgat ttttggacta
     4441 gaagtcgctg ctccgtagcc tgctgctcca ataacaaaca agacaaaaat agttgtcact
     4501 tcaaggttgc ctgtgaaaat tccatagata aggaagacgg ccaacaaacc attatagatt
     4561 ccctggtttt taaatagggt tgtcacagac ttacgttgta attcttcctt ctccatattg
     4621 aatacgcgac tagtagagtc tgaatgggtc gcaaaggttt ccaaatacat gatatatagg
     4681 tgctcaatag ctacgatact agctagaata gtggtaaaaa tagacataat gtctcctctc
     4741 aaaaaattat ataggtagat tttgcaagcc aatcaacagt ttatcaagag tggctaggaa
     4801 ttgctcgctc tcctcaacag ttagaatctg attcatcgaa gttttgatag cttggtggtg
     4861 ggcagaagga ttcgtgatta gcgcttctct agcctgctca gttggccaga caagtacttc
     4921 tctctgattg tcaggatttc gcttgcgaat gatatagccg ctctcttcca acaatttgag
     4981 atgacgcgta accgctgcgc ggtctatctg aagccgctcc tgcaaagcca actgatgcag
     5041 gggtgcatct tttagtaatg tttgcaatat tgaatagcgt gtcaaactga ttcccaactg
     5101 cttttcaaaa agattggaaa tcgtctcatc tgccactttc aatctgtata acaaatcttc
     5161 catttcttgc attatctacc tcttttgatt gattaatcaa tcattgataa gtcaattata
     5221 ctataatccg aacaattttc aactattttg cctctctata aaaaaatatg ttgcctacac
     5281 ctatattttg ttataattaa gaaaataact agcagaggga tgggacttac ttatggaaaa
     5341 cactcctaga ctaaacttgg tcatagacgt gctcatgctc gctggtgcca ttttggcaca
     5401 aagcggtgca gaaattcacc gagttgaaga taccatgatt cgtattgcac attcacacgg
     5461 aattgaacag gctaatgtcc ttgccatccc tgctgctatc tttttctcca tcgaccatac
     5521 gaatatttcg cgtatgaaac gcattgtcaa ttcaaattac gacatgcaga aagtttgtga
     5581 cgtcaaccaa atctcccgtt ccctagccaa tgaagaaatc agcctagaag aagcttggag
     5641 ccaattaaat gcaatcaaac aacaaaagcc accttataac aatatccaaa taaccttagc
     5701 tgccgtagta gccgctccat tttttaccat catgttcgga ggttctcttt tagatgcagc
     5761 aggtgctgtc ctcgcaacag ctttagcttt ccctatctca ctaattgtcg agcgctatat
     5821 tggaattgat tttgtcacca ccttcgttgg cgcctttgtc ttctcactca cctcttattt
     5881 attaagcgca accactactc tccccattaa tcctaacctg attaatgcgg gggcagtcat
     5941 gccttttgta ccagggattg cttttacaaa tgctgtaaga gacctcatga ccaatcatat
     6001 caatacaggg atgaccaaat ttttccaaac tgcgcttatt atcttatcgc tcggtgcagg
     6061 tacaactgtg gcctttatgc ttgtgaggta atctatgaca ccagttcact tcttattaca
     6121 ggcactcgcc tcttatatcg ctatcgcagc cttcttaatt gttctcaatg ttcaaagaaa
     6181 aatgctggtc cctggtggac tcctaggcat gcttgtttgg cttatttatc ttttactatt
     6241 ggaaccaaca aatgtcttga ttgctacctt tttcgcggca attatcggat cctgtgtcag
     6301 tcaaatcatg agtatttggt taaaaacacc ctccgtcatc ttttcattgg ctatacttgc
     6361 accacttgtt ccaggctatc gagcctacat gaccacgact tatttcgttt ctggagacca
     6421 cgctcaagcc ctaaccaata tcactaccgt cttaacttta gccttagtca ttcccatcgg
     6481 tatggccagc ggaacaatat tattacgcct ttataaggtt cttcgaaccg gcaaaaaagc
     6541 agcctaaccc cttgaaaata aggggccttt ttgttaaaat gaaaacttat caaaggagat
     6601 gctatgaacc aaaacgtaac caatctaaaa cttgctgaac gtggtgcctt gctatctatc
     6661 ggtgcctata ttgtcttatc aggtatcaaa ctcgttgcag gacagctctt tcattcagat
     6721 gccttacgag cagatgcctt caataatatt tctgatatta tcggtaatat cgctgtactt
     6781 attggactaa aaatggcaca gaaaccagct gataccgatc ataaatttgg acactggaaa
     6841 atggaagact tggcaagctt gattacatcc ttcatcatgt ttgtcgttgg tttccaagta
     6901 ctctatgata ccctccaaaa gctaatcagc aattcttctg tcgaagtaga cattatgggg
     6961 gctattgtcg gaattttttc tgcactagtt atgttagctg tttatttata taataaccgc
     7021 ttggcaaaaa aagtacgttc caaggcctta gaagcagctg ccaaggataa tctatccgat
     7081 gcagtcactt ccataggaac ttctatcgcg atctttgctg cggctttcaa cctccctatt
     7141 gtcgataaaa ttgcagccat catcattacc ttctttatcc taaaaacagc ctacgatatt
     7201 tttatggaaa gcttctttac cctatctgat ggatttgatg aaaacttatt gaaaaagtac
     7261 gaggaagata ttctcaaact cccaaaaatc gtttctgtca aatcacaacg tggtcggact
     7321 tatggagcca atatctacct tgatgttgtt ttagaaatga atccagactt atccgtttat
     7381 gaaagtcatg aagtgactga gcaggttgag caactcctaa cactcaaaca tggagttttt
     7441 gatgtggata ttcacgtaga accatctgaa attccacacg atgagatgta tgagcatgtg
     7501 tacgacaaac tcttccgttt tgaaacagag atacaagcac atgctaatgg gtatgaagaa
     7561 ttgattgacg accagtatct cttaattgat gccaaaggcc actaccgaaa taaaactcag
     7621 atacttgccg accaccccat ccaaacgacg tatttatcaa attaccaaat gacatctatt
     7681 agccataaat caaaattagt tacgtttgaa attggcgaat atgttcatac gtcactttgg
     7741 cgccgtcatg aaaattggac tgttattttc catcaaattt caaagaaaca agcctctcag
     7801 atcgaagcta ctgaaaaaat ctctgatttg cataatccat aataaaaaat aggaattttc
     7861 cctggattat agggagaatt cctatttttt aaccttccct ttgagctatt atcagtcttt
     7921 tatactcaat ttgtagttat ttagtttttg tttagtatat gataaaagct aaataactat
     7981 agaagattag agcttactgt tttgggattt cattgattat tatattataa agaaaaagcc
     8041 tctacccgaa taaatatttg agtagagatt ttaactgaat gatattgccc attggcagg
//
LOCUS       DBNFEG010000096         8080 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.096, whole genome shotgun sequence.
ACCESSION   DBNFEG010000096 DBNFEG010000000
VERSION     DBNFEG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8080
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.096"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            15..1787
                     /locus_tag="AC1PNG_001380"
     CDS             15..1787
                     /locus_tag="AC1PNG_001380"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 35 protein"
                     /protein_id="HIP6701882.1"
                     /translation="MKEFYIGDQFYLDGEPFKILSGAIHYFRVHPDDWYHSLYNLKAL
                     GFNTVETYVPWNMHEPRKGEFCYEGILDIERFLKLAQELGLYAIVRPSPYICAEWEWG
                     GLPAWLMKEELRVRSSDSVYLQHLDEYYASLIPKLAKLQLAQGGNVLMFQVENEYGSY
                     GEEKEYLRSVAGLMRKHGLTAPLFTSDGSWRATLRAGTLIEDDVFVTGNFGSKARENF
                     ANMTAFFNEHQKNWPLMCMEFWDGWFNRWGDEIIRREPEEMVDSVMECIELGSLNLYM
                     FHGGTNFGFMNGCSARGQIDLPQVTSYDYDAILDEAGNPTKKFYILQQRLKEVYPELE
                     YAEPLVKEAKAFSDVSLHDKVSLFATLENVSGCVKGFYPKNMEELDQSTGYILYRTEL
                     ERDKTEAERFRVVDARDRIQIYADGKFVATQYQTEIGDDVELDFKDDKLTLDILVENM
                     GRVNYGHKLTAPTQSKGLGRGAMADLHFIGHWETYPLPLDSVEDLDFSKGWEEGQAAF
                     YRYQFELDELADTYLDMTGFGKGVVFVNNVNIGRFWEKGPILYLYIPKGYLKKGANEI
                     VVFETEGKYREKIHFSQRPVIKDL"
     gene            1841..2326
                     /locus_tag="AC1PNG_001381"
     CDS             1841..2326
                     /locus_tag="AC1PNG_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HIP6701883.1"
                     /translation="MAIIATRIDGRLIHGQVANLWTTKLNIGRIMVIDDAVAQNDIEK
                     QGLKLACPPGVKLSILPIEKAANNIKEGKYDSQRLLIVARRPENFLRLVEYGVELPEL
                     NVGNMSQTPETRSVTRSINVVDKDIADFDALAAKGVKLFAQMVPGDSPKDFMPLLDKV
                     R"
     gene            2365..3267
                     /locus_tag="AC1PNG_001382"
     CDS             2365..3267
                     /locus_tag="AC1PNG_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HIP6701884.1"
                     /translation="MMQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGN
                     MEVGLLIGASLQLVVLGVGTFGGASRIDATSGAVLATAFSISQGLDPEIAISTIGVPV
                     AGLLVYTDILGRFTTTFFAHRVDAAIERFDYAGIERNYLLGAIPWALSRALPVFLALA
                     FGGGIVETLVTTIELPEYKWIAAGLTLAARMLPGLGFAILLHYLPLKRNLHYLAVGFA
                     LTAMLTVLYGNVSALGGAVAGIVGTLPEDAGVSFVNNFKGLSMIGIAIVGAFLSVIHF
                     KNSQKVTVVAPSNSESGEIEDDEI"
     gene            3254..4072
                     /locus_tag="AC1PNG_001383"
     CDS             3254..4072
                     /locus_tag="AC1PNG_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIP6701885.1"
                     /translation="MTKSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKLHTQFFNTSPFFHTIITGIDLALEESDGVASKDAVNGVKT
                     GLMGPFAPIGDSIFGSLVPAIMGTVAATMGKEGSPVGIFLWVAVAIAYDIFRWKQLEV
                     AYKEGTKLITTMRDRLTALVDAASVMGVFMMGALIATMINFEVTWAPAIGEKVIDIQD
                     ILNTIFPRLVPAIFTGFVFWLLGRRGMTSTKAILIIIVLALGLSAIGHFLFGMV"
     gene            4072..4473
                     /locus_tag="AC1PNG_001384"
     CDS             4072..4473
                     /locus_tag="AC1PNG_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIP6701886.1"
                     /translation="MVRSLVLVSHGIFCEELKKSTEMIMGPQEDIYTVALLPEEGPED
                     FQKKFEETIAGLEDFVVFADLLGGTPANVVSRKLIEGGQFDLYAGMNMPMVIGFLNGV
                     LLGEAVDYVEFGTSNLVHVNSLLTSDEDDDE"
     gene            4689..5690
                     /locus_tag="AC1PNG_001385"
     CDS             4689..5690
                     /locus_tag="AC1PNG_001385"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIP6701887.1"
                     /translation="MIEVKTFGGQAKLYCLENKNGMQVTLTDFGARVVEVLLPVEENG
                     GLRNVSLAAKSDDDYRKTDLYPGSSIVPVAGRISGAQAEIKGTSYQFTENEPGRTLHG
                     GIDTANEQYWDVALDHEKNQVTFGIVLKDGFNGFPGDVRVEAIYGLTDQNELTIDYRA
                     VSSKDTIFNPTNHIYFNLTGDFQQSVAEHEIRIAANRYAPLGEDNLPTGVLEDVTGTP
                     FDFRERARFAQGFDSQHPQNILVKGYDHPWLLEQVEVPIEVVSPDGKISLRVRTNQAS
                     VVIYTYNYPVEALAAYHGAFSLECQALPNACNLGGFGSILLEKDEEFLSQTVYQFTW"
     gene            5798..6052
                     /locus_tag="AC1PNG_001386"
     CDS             5798..6052
                     /locus_tag="AC1PNG_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HIP6701888.1"
                     /translation="MTKEQEFLKEFEAWVNTQVMVNEMAVEESRRVLEEDKDERAADA
                     YIRYESKLDAYRFIQGKFANYHAGKGFHDLPDELFGQRHY"
     gene            6067..6453
                     /locus_tag="AC1PNG_001387"
     CDS             6067..6453
                     /locus_tag="AC1PNG_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIP6701889.1"
                     /translation="MAKKKVNRKKQLKKQLADLKRAGRVGLERAAEVVETVAHKAEAV
                     VEHAVEQVKEVVAEVTSSATSLEDFLALPELEGIAAARLETFYEAGIQSVADFANHTE
                     KELLALKGIGPATIKQLKEKGIELKA"
     gene            6695..7084
                     /locus_tag="AC1PNG_001388"
     CDS             6695..7084
                     /locus_tag="AC1PNG_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIP6701890.1"
                     /translation="MLHHVEIYVSNLETSRAFYDFLLTKLGYSLYQEWEEGLSYKKAE
                     QYLVFVQTPQDFLKAGYHRCRTGLNHLAFHVGTPDDVDQWRKEFLTRRVKLLYNDRYP
                     YAGGPDHYALYLEDPDGIKIELVGEVT"
     gene            7081..7656
                     /locus_tag="AC1PNG_001389"
     CDS             7081..7656
                     /locus_tag="AC1PNG_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIP6701891.1"
                     /translation="MNLVIIGAQASGKMTIGQEVAKLTGMTLFHNHDSIDFSLRFIPE
                     FSEDMFDLNTRITFAVYDVFARSGRPLIGTALINFKNLIEVQFLTTVQTIFHYHGQEI
                     LFVELETALEERLRRNRTENRLTHKPLKRHIEVSEAEILSTADTCQYTSLGIPEGIQH
                     YLKINNTHLTANEVAQLIIKKMEELEQKQTK"
     gene            7820..>8080
                     /locus_tag="AC1PNG_001390"
     CDS             7820..>8080
                     /locus_tag="AC1PNG_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701892.1"
                     /translation="MKMLNKFTENSKDLRNELTSWFVIISLAFLLFLSIPWLVQKTLY
                     FTLSKPFEAQRVAITKSNKTSDTASGVEGTYINNIPFNDFEKL"
BASE COUNT         2305 a         1404 c         1934 g         2437 t
ORIGIN      
        1 catcggagga agaaatgaaa gaattttata ttggagacca attttatttg gacggtgaac
       61 cctttaagat tttatcaggc gcgattcatt attttcgtgt ccatcctgat gattggtacc
      121 attctttgta taacttaaag gcccttggtt ttaatacggt tgaaacctat gtcccttgga
      181 atatgcatga acctcgaaag ggtgagtttt gttacgaggg tatattggat attgagcgat
      241 ttttgaaatt ggctcaagaa ttagggcttt atgcaattgt acgtccttcc ccttatattt
      301 gtgcggagtg ggaatggggt ggcctccctg cttggttgat gaaggaagag cttagagtac
      361 gttcgagtga ttcggtgtac ttgcagcact tagatgaata ttacgcttct cttattccta
      421 aattggccaa gctccaactc gcacagggtg gaaatgtact tatgttccaa gtggaaaatg
      481 agtatggttc ttatggtgag gagaaggagt atctaagatc ggtggctggc ttgatgcgta
      541 aacatggtct aactgctccc ttgtttacct cagacggttc ttggcgagca acattacgtg
      601 caggaacact gatagaggac gatgtatttg taacaggaaa ctttggttct aaagctagag
      661 agaattttgc aaatatgacg gcattcttta atgaacatca gaagaattgg ccactcatgt
      721 gtatggaatt ttgggatggt tggttcaatc gttggggcga tgaaattatt cgtagagagc
      781 cggaggaaat ggttgattcg gttatggaat gtatcgaatt gggctccttg aatctgtaca
      841 tgttccatgg tggaacaaat tttggtttca tgaatggttg ctctgctcgt ggtcaaattg
      901 atttgcctca ggtaacttct tatgattatg atgctatatt ggatgaagct ggaaatccta
      961 ctaagaagtt ctatattttg caacaacgtt tgaaagaagt ctatcctgaa ttagagtatg
     1021 cagaaccgct tgtaaaagag gcaaaagctt tttcagatgt gtcgcttcat gataaggtga
     1081 gtttatttgc tactctagaa aatgtaagcg gttgcgtaaa agggttttat cctaagaata
     1141 tggaagaact tgatcagtca acaggttata ttttatatag aacagagttg gagcgggata
     1201 agacagaagc ggaacgtttc cgagtagttg atgcgcgtga ccgtatccaa atttatgcag
     1261 atggaaaatt cgttgcaact cagtatcaga cagagattgg tgacgatgtt gaattggatt
     1321 ttaaagatga caagctaaca ttagatattt tggtagaaaa tatgggacgt gtcaattatg
     1381 ggcacaaact aacggctcct acccagtcta aagggcttgg tcgaggtgcg atggctgatt
     1441 tacattttat tggtcattgg gaaacctatc ctttgcctct tgattcagtt gaggacctgg
     1501 attttagtaa gggctgggag gaaggtcaag ctgctttcta tcgttatcag tttgaactgg
     1561 atgaacttgc ggatacttac ttagatatga caggttttgg taagggagtt gttttcgtta
     1621 ataatgtaaa tattggacgt ttttgggaaa aaggaccaat cctctatctc tatattccaa
     1681 aaggatactt aaagaaagga gcgaatgaaa tagttgtctt cgagacagaa ggaaaatata
     1741 gagagaagat tcatttttca caaagacccg ttataaaaga cttgtagaaa ttttaatcgg
     1801 tttctgctca aaagatttat gaagaaaagg agaacactgt atggcaatta ttgctacgcg
     1861 tattgatggt cgtttgattc acggtcaggt tgctaactta tggacgacga aattgaacat
     1921 cggtcgtatt atggttatcg atgatgctgt tgctcaaaat gacattgaaa aacaaggact
     1981 gaaattagca tgtcctccag gcgtaaaatt atcgatttta ccgattgaaa aggctgctaa
     2041 taatattaag gagggcaagt atgatagtca acgtctcttg attgttgctc gtcgtccaga
     2101 aaacttcctt cgcttggtag agtatggggt tgagctccct gagttgaacg tagggaatat
     2161 gtctcaaact ccggaaactc gttctgtaac tcgttctatt aacgttgtag ataaggatat
     2221 tgcagatttt gatgccttgg cggctaaggg tgtaaaatta tttgcacaaa tggtacctgg
     2281 cgattcacca aaagacttta tgccattatt ggataaggtt agataacatt cggatatttt
     2341 tgaaaaattt aaaggaggat cactattatg caatggtggc aaatattact tctaacgctc
     2401 tactctgctt accagatttg tgacgagctg acaattgtat cgtcagcagg ttcaccagtc
     2461 tttgcagggt ttatcactgg tttaatcatg gggaacatgg aagttggctt gctaattggt
     2521 gcaagtttgc agctagttgt cttgggagtt ggtactttcg gtggtgcgtc acgtattgat
     2581 gcaacatctg gtgcggttct tgcgacagct ttctcaattt cacaaggttt agatccagaa
     2641 attgctattt ccactattgg cgtaccagtt gcaggattgt tggtctatac agatattttg
     2701 ggtcgtttca ccactacttt ctttgctcat cgtgtagatg cagcaatcga acgttttgac
     2761 tatgctggta ttgaacgcaa ctaccttctt ggtgcaattc cttgggcact ttctcgtgca
     2821 cttccagttt ttcttgccct tgcttttggt ggtggtattg tagaaactct tgtcactacg
     2881 attgagttgc ctgaatacaa atggattgca gcaggcttaa ctcttgcagc tcgtatgctt
     2941 ccaggtcttg gatttgcaat cttacttcac tatcttccac ttaaacgtaa ccttcactac
     3001 ttggcagtag gtttcgctct tacagctatg ttgactgttc tttacggtaa cgtatcagct
     3061 ttgggtggtg cagttgctgg tatcgttggc actcttcctg aagatgcagg cgtaagcttt
     3121 gtcaataact tcaaaggttt gtcaatgatt ggtatcgcta ttgtgggtgc attcctttca
     3181 gtgattcact tcaaaaatag ccaaaaagta actgtggttg ctccatcaaa ttcagaaagt
     3241 ggggaaattg aagatgacga aatctaatta caaattaaca aaagaagatt ttaatcaaat
     3301 caacaaacgt agcttgttca ctttccaact tggttggaac tatgagcgga tgcaggcatc
     3361 tggctacctc tacatgattt tgccacaatt gcgtaaaatg tatggagatg gtactcctga
     3421 gttgaaggaa atgatgaaat tgcatactca gttcttcaat acttcaccat tcttccatac
     3481 catcatcaca ggtattgact tggcacttga agaaagtgat ggcgttgctt ctaaagatgc
     3541 ggttaacggt gttaagacag gtttgatggg accatttgct ccaatcggtg actcaatctt
     3601 tggttctctt gttccagcga ttatggggac tgtggctgct actatgggta aagagggatc
     3661 tccagttggt atctttcttt gggtagcagt agccattgcc tatgatattt tccgttggaa
     3721 acagttggaa gttgcctaca aagaaggtac taagttgatt acaaccatgc gcgatcgctt
     3781 gacagctctc gttgatgcag catctgtaat gggtgtcttc atgatgggag ccctgattgc
     3841 aactatgatt aactttgaag tgacttgggc tccagctatt ggagaaaaag tgattgatat
     3901 tcaagacatt ttgaatacca tcttcccacg cctagtacct gcaatcttta caggatttgt
     3961 cttctggctt cttggtcgtc gtggtatgac ttcgactaaa gctatcttga ttattattgt
     4021 gcttgcactc ggcttgtcag caatcggtca cttcctattt ggtatggtat aatggttaga
     4081 agtttagtat tagtaagtca tggcatcttc tgcgaggagt tgaaaaaatc tactgaaatg
     4141 attatgggtc ctcaagagga catctacaca gtagctttat taccagaaga aggtccagaa
     4201 gacttccaga aaaaatttga agaaacaatt gcaggattgg aagatttcgt tgtcttcgca
     4261 gacttgttgg gcggtacacc agccaacgtt gtatcacgaa aattgattga aggtgggcag
     4321 tttgacctct atgcagggat gaacatgcca atggttatcg gtttcttgaa cggcgtcctg
     4381 ctgggagagg ctgtggacta tgtagaattt gggacaagca acctagttca tgtcaatagt
     4441 ctattaacaa gtgatgaaga tgacgatgag tgaaacagtt tggggaactg tttcaactgg
     4501 tcaccttgaa atgaaagagt gaccaaagac agcccgagcc tagaaataag agagtgaggc
     4561 taactcagcc gattgttaca gacggatttg attttcgtta attataaaat gttctatagt
     4621 tgataggctt taagtatcct gagggagttg caatagtgac tccctcattt ggcttgtgag
     4681 gaaaagaaat gattgaagtg aaaacatttg gtggacaagc aaaactgtat tgcttggaaa
     4741 acaagaatgg gatgcaggta accttgacgg attttggtgc cagagtggtg gaagtgcttt
     4801 tgccagtaga agaaaatggc ggtctacgga atgtcagttt ggctgccaag tcggatgatg
     4861 attaccgcaa gacagatttg taccctggtt caagcattgt cccagtagcg gggcgtattt
     4921 caggtgctca ggctgagata aagggaacca gttatcagtt cacagaaaat gagccaggtc
     4981 gtaccttgca tggtgggatt gacacggcaa atgaacagta ctgggatgtt gctctagatc
     5041 atgagaaaaa tcaagttacc tttgggattg tcttaaaaga tggtttcaat ggttttccag
     5101 gagatgtgag agttgaggct atttatggtc taacagacca gaatgagttg acgatagatt
     5161 atcgggcggt gtctagcaaa gatacgattt tcaatccgac caatcacatc tatttcaact
     5221 taacggggga ttttcagcag tcagtggcag agcacgagat tcgaattgcg gctaatcgct
     5281 atgctccgct tggtgaggat aatctgccaa ccggtgtctt agaggatgtg acaggaacac
     5341 cgtttgactt tagggagaga gcaagatttg ctcagggctt tgacagccag caccctcaga
     5401 atatccttgt gaaaggttac gaccatccat ggcttttgga acaggtagag gttccgattg
     5461 aagttgtcag cccagatggg aaaataagtc tgcgcgtgcg aaccaatcag gcatcagttg
     5521 tcatctacac ctacaattat cctgttgaag ctttggctgc ctaccatggt gcctttagct
     5581 tggaatgcca ggctctgcca aatgcttgca acctaggcgg ttttggttct attttgctgg
     5641 aaaaagacga ggaattcttg tcccagactg tttatcaatt tacttggtaa gaaggagagc
     5701 tctaactgtg ttgcagttag gcttttcttg tgttttctaa aaaatatggt ataataaacc
     5761 ggtgtgtttc aaacgaagca gacgaaagag gaaaataatg acaaaagaac aagaattttt
     5821 aaaagaattt gaggcttggg tcaacactca ggtcatggta aacgagatgg cggttgaaga
     5881 aagtcgtcgt gtcttggaag aagacaagga tgagcgtgca gcggatgctt atattcgtta
     5941 tgagagcaag ctggatgcct atcgattcat tcaagggaaa tttgccaact accatgctgg
     6001 caaaggtttt catgatttgc cagatgagtt gtttggtcaa aggcattatt aaggagagga
     6061 gttaacatgg caaagaaaaa agtaaatcga aaaaaacagt tgaaaaaaca attagcagac
     6121 ttgaaacgtg caggtcgtgt aggtcttgaa agagctgcag aggtagtaga gacagttgct
     6181 cacaaggctg aggctgtagt agagcatgct gtggaacaag tgaaagaagt tgtagcagag
     6241 gtgacttctt cagcgacttc attggaagac tttttggcac ttcctgagtt ggaaggcatt
     6301 gcggcagcac gtttggaaac cttctacgaa gcgggtattc aatctgtggc tgattttgca
     6361 aaccatacag aaaaagagct cttggctctt aaaggcatcg gtcctgcaac tatcaagcag
     6421 ttgaaagaaa aagggattga gttgaaagct taaatccctt gcttttcaag gtgcaatttg
     6481 ttacaataaa gccattcaga ggtgttttga tctccatttg tcacaggaag cggtggtact
     6541 gagaaatccg cacaaatgtc aaaactggtt gctgatgatg tgaatcgaat aagcagagtg
     6601 cagataatgt atctgaacaa gggtggtacc gcggaataac ttcgtccctg tctagcaagt
     6661 ctaggcgggg attttcattt ggaaaggaga aaatatgtta catcatgttg aaatttacgt
     6721 ttctaatttg gaaacctcgc gtgcatttta cgactttctc ctgaccaagc tgggatactc
     6781 gctctatcag gagtgggagg aagggctatc ttataaaaaa gcagagcagt acttggtctt
     6841 tgtccaaacg ccacaggact tcttaaaggc aggttatcac cgctgtcgca caggtctcaa
     6901 tcatctagct tttcatgtgg gaacacctga tgatgttgac caatggcgga aggaattttt
     6961 gaccagacga gtcaaattgc tttacaatga ccgctatccc tacgcgggag gaccagacca
     7021 ctacgccctc tatttggaag acccggacgg gattaagata gagttggtgg gggaggtaac
     7081 atgaatttag taattattgg agcacaagct tctggcaaga tgaccattgg gcaggaagtt
     7141 gcgaaattga cagggatgac cctttttcat aatcacgatt cgattgattt tagcctacgt
     7201 tttattcctg aattttctga ggatatgttt gacctcaata cacgtattac atttgctgtt
     7261 tatgatgtgt ttgctaggtc aggccgccca ctaattggaa ctgccttgat taattttaaa
     7321 aatctcatag aggttcaatt ccttacaact gttcagacga tttttcatta tcatggtcag
     7381 gaaattttat ttgttgagtt ggagactgct ttggaagaac gtctgcggcg taatcggaca
     7441 gagaatcgac tgacccataa acctttgaaa cgacacattg aagtttcaga ggctgagatt
     7501 ctctctacag ctgatacatg tcaatatacc tcattaggca ttccagaggg cattcagcat
     7561 tatctcaaaa ttaataatac tcaccttact gctaatgaag ttgctcaact gattatcaaa
     7621 aaaatggagg aacttgaaca aaaacaaacg aaatagcctt tccgttgagg ggcgaaacag
     7681 aatataatcc agataggcat aaactatttc tattagtact atcagttcag tcctgctact
     7741 atattacatc atgcttatct catggagcat actattttgc gtgcagtgta ttaaaatatg
     7801 aattaggagt atactgtcta tgaaaatgtt aaacaagttc acagagaatt caaaagattt
     7861 gagaaatgag ttgactagct ggtttgtcat catttcgcta gccttcttat tattcttgag
     7921 cattccttgg ttggtgcaaa agactttata ttttacttta tcaaaaccat ttgaagcaca
     7981 aagagtagcc atcacaaaat caaacaaaac ttcagatact gcctctggag ttgaagggac
     8041 ctacatcaat aatattccct ttaatgattt tgaaaaattg
//
LOCUS       DBNFEG010000097         8079 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.097, whole genome shotgun sequence.
ACCESSION   DBNFEG010000097 DBNFEG010000000
VERSION     DBNFEG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.097"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..578
                     /locus_tag="AC1PNG_001391"
     CDS             <1..578
                     /locus_tag="AC1PNG_001391"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIP6701893.1"
                     /translation="YEKNPTIAHDIHEIHVNLLDTPMSFMTDAGVFSKKMVDYGSQVL
                     LKTLHFEKGASVLDVGCGYGPIGLTLAKVFNTKTTLIDINSRALDLATKNAERNGVIA
                     KIYQSNIYENVDETFNHIVSNPPIRAGKSVVHEVIAGAFQRLEAHGTLSIVIQKKQGA
                     PSAKAKMEEVFGNCQIIKKDKGYYILESVKE"
     gene            575..1852
                     /locus_tag="AC1PNG_001392"
     CDS             575..1852
                     /locus_tag="AC1PNG_001392"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HIP6701894.1"
                     /translation="MRAVDLIQKKRDGLELSSEEIKWLIDGYVAGTVPDYQMSALAMA
                     IYFKGMSTREISDLTMSMVVTGEQIDLSAIPGIKVDKHSTGGVGDKVTLILAPLVASF
                     DIPVAKMSGRGLGHTGGTLDKLESIKGYQIEVSQADFIKQVQDTGVAVIGQSDNLVKA
                     DKLLYALRDVTATVDIIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVEEARVLA
                     QTMVDLGKAVGRKTVAVLTDMSQPVGTSIGNRLEILEAIEILQGKGREDVTEFICELA
                     QIMLGLANVEKSIEEVRQHIDNGAALKKFEEMVRAQGGDLEDLYRPVQVTQQVPVLAE
                     EDGYIVGLPALEFGLFAMKLGAGRAVKTDNLDYETGIVFHKKVGEAVSKGEQIATIYA
                     NENISENMLTNFQKNVKIDKESVKTKEIIEIIS"
     gene            1868..2530
                     /gene="deoC"
                     /locus_tag="AC1PNG_001393"
     CDS             1868..2530
                     /gene="deoC"
                     /locus_tag="AC1PNG_001393"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIP6701895.1"
                     /translation="MKLNKYIDHTILKPETTQEQVEKILAEAKEYDFASVCVNPTWVA
                     LAAESLKDSDVKVCTVIGFPLGANTPAVKAFETKDAISNGADEIDMVINIGALKTGNY
                     DLVLEDIKAVVAASGDKLVKVIIEACLLTDDEKVKACQLSQEAGADYVKTSTGFSTGG
                     ATVADVALMRKTVGPDMGVKASGGARSYEDAIAFIEAGASRIGASSGVAIMNGAQADG
                     DY"
     gene            2517..2906
                     /locus_tag="AC1PNG_001394"
     CDS             2517..2906
                     /locus_tag="AC1PNG_001394"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIP6701896.1"
                     /translation="METIDLIELAVKNSQKAYVPYSKFPVSAVLVAETGEIFTGVNIE
                     NASFGLTNCAERTAIFKAISEGVKDFSEIVIYGKTEKPISPCGACRQVMAEFFDKDLK
                     VTLVAKDKSTVETTVGELLPYSFTDLT"
     gene            2987..4054
                     /locus_tag="AC1PNG_001395"
     CDS             2987..4054
                     /locus_tag="AC1PNG_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIP6701897.1"
                     /translation="MNKKLVGLGLAAVAAVSLAACGNRASKSSSSEASSSADTSVKAA
                     IVTDIGGVDDRSFNQSAWEGLQEWGKAASLSKGNGYDYFQSASESDYITNLDSAVAGG
                     YNLVFGIGFALESAIAEVAPNNPNTHYVIVDSVVPDQDNVVSVGFADHEASYLAGVAA
                     AKSTKTNHVGFIGGMEGVIIDRFEAGFVAGAKSVNKDIKITVDYAGSFGDAAKGQTLA
                     AAQYAAGADVIFHASGGTGNGVFAAAKAENETRNEADKVWVIGVDRDQSAEGTYTSKD
                     GKSSNFVLASTLKQVGTSVKDIATKAAAGDFPGGQVLQFSLKDKGVELAETNLSEDAS
                     KAVADAKQAILDGKVEVPEKP"
     gene            4249..5784
                     /locus_tag="AC1PNG_001396"
     CDS             4249..5784
                     /locus_tag="AC1PNG_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6701898.1"
                     /translation="MTKDYVIEMRNITKIFGEFVANDHINLNVRRGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIVINGQSVKIDSPSKAAQLGIGMVHQHFMLVDAFTVAENI
                     ILGSETTKGGVIDLKKAIAEIKELSAKYGLEVDPTAKVADISVGAQQRVEILKTLYRG
                     ADLLIFDEPTAVLTPAEIAELLKIMKALVEEGKSIILITHKLDEIRAVADSVTVIRRG
                     KSIETVAVEGATNQDLAEWMVGRSVSFKTEKVEASPKEVILSIKDLVVNENRGIPAVK
                     GLSLDVRAGEVVGIAGIDGNGQSELIQAITGLRKVKSGSITIKGEEVVGKTPRKITEM
                     QVSHVPEDRHRDGLVLPMTVAENFALQTYYKEPISKNGILNYNIINEKARALMEEFDV
                     RGASELVPIKALSGGNQQKAIIAREIDRNPDLFIVSQPTRGLDVGAIEYIRKRLIAER
                     DKGKAVLVVSFELDEILDVSDRIAVIHDGVIHGIVEPAKTNKQELGVLMAGGHIEKGE
                     EHA"
     gene            5777..6838
                     /locus_tag="AC1PNG_001397"
     CDS             5777..6838
                     /locus_tag="AC1PNG_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6701899.1"
                     /translation="MLNKYKNLALPLMAVVSGILLGAIVMLVFGYDPIWGYEELFYSA
                     FGNIKSIGEIFRAMAPLVFTALGFAVASRAGFFNVGLSGQAYVGWVFAGWFALANPDL
                     PRPVLILGTVLIAMLAGGIAGAIPGILRAYLGTSEVIVTIMMNYILLYSCNYIIRDVF
                     ADSLMKNTDSTVNVSANASYQTEWLRALTDNSRMNIGIFFAIIAVFVIWFLLTKTTLG
                     FEIRSVGLNPTASDYAGMSAKRTIILSMIISGALAGLGGAIQGLGTFQNVYIQSGNLD
                     IGFNGMSVALLASNSPLGIPLAAFLFGVLSVGAPGMVRAQIPPELINVVTASIIFFIG
                     VKFIFEQLLKSRNKAKGAK"
     gene            6840..7793
                     /locus_tag="AC1PNG_001398"
     CDS             6840..7793
                     /locus_tag="AC1PNG_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6701900.1"
                     /translation="MNVSILALLISQMLIYSAPLIFTSLGGVFSERGGIVNVGLEGIM
                     VIGAFAGVVFNIEFAGTFGNATPLLAIIVGGLAGVVFAAIHAMATINFRADHVVSGTV
                     LNLLAPALGVFLVKVIYNKGQTDSITESFGKFSFPILADIPIIGEIFFKNTSLMGYVA
                     IATAFLAWFILYKTKFGLRLRSVGEHPQAADTLGINVYAMRYAGVLIAGFLGGVGGAV
                     SAQSVNINFSATTIVGSGFIALAAVIFGKWNPIGAMLASLFFGLSQSLAVVGGQLPGL
                     KDIPTVYLQIAPYLITVIALSAFFGKAVAPKADGINYIKSK"
BASE COUNT         2328 a         1365 c         1907 g         2479 t
ORIGIN      
        1 attacgaaaa aaatcctaca atagcacatg atattcatga aatccacgta aacttactag
       61 acaccccaat gtctttcatg acagatgcag gtgtttttag caagaagatg gttgattatg
      121 gtagtcaagt gcttttaaaa acccttcatt ttgaaaaagg ggcatcggtg ttggatgttg
      181 gttgtggtta tggaccaatt ggtttgacac ttgctaaggt gtttaacacc aagacaacac
      241 tgattgatat taatagcaga gctttggatt tagctacaaa aaatgcagag aggaatggtg
      301 ttatcgctaa aatttatcaa tccaatatct acgaaaacgt ggacgaaaca ttcaatcata
      361 ttgtttcaaa tccacctatt cgggccggaa aatcggttgt acatgaagta attgctgggg
      421 cctttcaacg cttagaggca cacggaacct tgtccattgt cattcaaaag aaacaaggcg
      481 caccgagtgc taaagctaag atggaagagg tattcggcaa ctgccagatt ataaagaaag
      541 ataagggtta ctacatcctt gaaagtgtga aagaatgaga gcagttgatt taattcaaaa
      601 aaagcgtgat ggcttagaac tatcgtctga agaaatcaaa tggttgattg atggatatgt
      661 agctgggaca gtaccggatt atcaaatgtc agccttagcg atggcgattt attttaaagg
      721 gatgtcaacg cgtgagattt ctgatttgac gatgtccatg gttgtaacgg gtgaacagat
      781 tgatttgtct gcaattccag gcatcaaggt agataagcat tctacaggtg gtgtcggaga
      841 taaggttaca ctcattttag cgccattggt agcaagtttt gatattccag ttgcaaagat
      901 gagcggtcgt gggcttggtc atactggtgg aaccttggat aagttagagt caatcaaagg
      961 ataccagatt gaagttagtc aagcagattt catcaagcaa gttcaagata ctggtgttgc
     1021 agttattggt cagtcagata atcttgtcaa agctgataaa ctactctatg cccttcgtga
     1081 cgtgacagca accgttgaca ttattccgct gattgccagc tcagtaatgt ctaagaaaat
     1141 tgccgcaggt gcggatgcga ttcttctaga tgtcacggtg ggagaaggtg ccttcatgaa
     1201 gactgttgaa gaagcacggg ttctagctca aactatggtg gatcttggga aagcggttgg
     1261 tcgtaagaca gttgctgttt taactgacat gtctcaacca gttggaactt cgattggaaa
     1321 tcgtttggaa attttagagg ctattgaaat tttacagggt aaaggtcgag aagatgttac
     1381 cgaatttatc tgtgaactag cccaaattat gttggggctt gctaatgttg agaaaagcat
     1441 tgaagaagta cgtcaacata ttgacaatgg tgcagcattg aagaaatttg aagagatggt
     1501 ccgtgctcaa ggtggtgact tagaagattt atatcgtcca gttcaggtta cacagcaagt
     1561 tccagttctt gcggaagagg atggctatat tgtgggactt cctgctttgg aatttggtct
     1621 ctttgcaatg aaacttggtg caggtcgtgc agtcaagact gacaatttag attatgaaac
     1681 aggtattgtt ttccataaga aagtggggga agctgtttct aaaggcgaac aaatcgcaac
     1741 gatttatgcc aatgaaaata tttcggaaaa catgcttaca aatttccaaa aaaatgttaa
     1801 aatagataaa gaaagtgtaa aaacgaaaga aatcatcgag attatttctt aatgtacgga
     1861 gaaagctatg aaattgaata aatatattga ccacacaatc ttgaaacctg agacaacaca
     1921 agagcaggtt gaaaaaattt tagcagaagc aaaagaatat gattttgcaa gtgtctgtgt
     1981 taacccaact tgggttgctt tagcagctga aagcttgaaa gatagtgatg taaaagtttg
     2041 tacggttatt ggtttccctc tcggagcaaa cacacctgct gtaaaagcgt tcgaaacaaa
     2101 agatgcaatt tcaaatggag cagatgagat tgacatggtt atcaatatcg gtgctctgaa
     2161 aactggaaat tacgatttgg ttttggagga tattaaagct gttgtagcag caagtggtga
     2221 taaactagtc aaagttatca tcgaagcatg ccttcttact gatgatgaga aagtgaaagc
     2281 ttgtcaatta tctcaagaag ctggtgcgga ctatgttaaa acatctacag gattttctac
     2341 aggcggtgcc acagtagcgg atgttgcctt aatgagaaaa acagtgggtc ctgacatggg
     2401 agtgaaggct tcaggtggtg cacgttcgta tgaagatgcc attgccttta tcgaagcagg
     2461 agcgtctcgt attggtgcgt catcaggcgt ggcaatcatg aatggagcac aggcggatgg
     2521 agactattga tttaattgaa ttagctgtaa aaaacagtca aaaagcatac gtaccgtact
     2581 caaagtttcc agttagtgca gttcttgtag ctgagactgg agaaatattt acaggtgtaa
     2641 acattgaaaa tgcaagtttt ggtttgacca attgcgctga aaggacagct attttcaagg
     2701 ctatttctga aggtgtgaaa gatttttctg aaatcgtaat ttatggtaaa acagaaaaac
     2761 caatatctcc ttgtggagct tgtcgccaag ttatggcaga attttttgat aaggatttaa
     2821 aagtgacctt ggtcgctaaa gataaatcga cagtcgagac gacagtcggg gaattactac
     2881 catattcctt tacagactta acatagtctg tatgacagtc atttgactgt agcaatatta
     2941 ctctgcaaca atttagttgc aaaaagattt ttaggagggt tcagaaatga acaagaaact
     3001 tgttggactt ggtctagcag ctgttgcggc tgtatctttg gctgcatgtg gtaaccgtgc
     3061 ttcaaaaagc agtagctcag aagcatcttc atcagcggac acatctgtga aagctgctat
     3121 cgttactgac attggtggtg ttgatgaccg ttcatttaac caatctgctt gggaaggtct
     3181 tcaagagtgg ggtaaagcag ctagtctttc caaaggtaat ggctacgatt acttccaatc
     3241 agctagtgaa tcagattata tcacaaacct tgattcagct gttgctggtg gttacaatct
     3301 tgtgttcggt attggttttg ccttggagag tgcgattgct gaggttgcac caaataatcc
     3361 taatacacac tatgtaatcg tggatagtgt tgttccagat caagacaatg tagttagcgt
     3421 aggtttcgca gatcatgaag catcatatct tgctggtgtt gctgcagcta aatcaactaa
     3481 aacaaatcac gtaggtttca tcggtggtat ggaaggtgtt atcatcgatc gctttgaagc
     3541 tggtttcgtt gctggtgcta agtctgtaaa caaagacatc aagattacag ttgactatgc
     3601 aggttcattt ggtgatgctg ctaaaggtca aactcttgca gctgctcaat atgctgcagg
     3661 tgcagatgtt attttccacg cttctggtgg tactggtaac ggtgtatttg cagctgctaa
     3721 agcagaaaac gaaactcgta atgaagcaga taaagtttgg gtaatcggtg ttgaccgtga
     3781 ccaatcagca gaaggtacat acacttctaa agatggtaag tcttctaact ttgtattggc
     3841 ttcaacattg aaacaagttg gtacttcggt taaagatatt gcaactaagg cagctgcagg
     3901 tgatttccct ggcggtcaag tacttcaatt ctcattgaaa gataaaggtg ttgaattggc
     3961 agaaactaat ctttcagaag atgcttctaa agcagttgca gatgctaaac aagcaatttt
     4021 ggatggcaaa gtagaagttc ctgaaaaacc ttaatgaatt tcttttcgac aattccgatt
     4081 cggcgacccg acggggcgcc gttttcgggc tgagattttt ctcagtaaag cctatcagct
     4141 atggtaggtt ttactgacaa aaatggtata gttgatgaag agtttttaag tactcaaagt
     4201 agtactgaag aattttcaga aggctatagt cagaaaggat aatagattat gactaaggat
     4261 tatgtcattg aaatgcgcaa tatcactaag atatttggtg aatttgtagc aaatgaccac
     4321 atcaatttga atgtcagacg tggtgaaatc catgcccttc ttggtgagaa tggtgcgggt
     4381 aaatctactc tgatgaacat gcttgctggc ttgttggaac ctacaagcgg tgaaattgtt
     4441 atcaacggtc aatccgtgaa gattgactct ccatccaaag cagcccaatt gggtatcggt
     4501 atggtgcacc aacactttat gttggttgat gctttcactg ttgctgagaa catcattctt
     4561 ggttctgaaa ccaccaaagg tggcgtgatt gaccttaaaa aagctatcgc agaaattaaa
     4621 gaactttctg ctaaatatgg tcttgaagtt gatccaactg caaaggttgc agatatttct
     4681 gtgggtgcac agcaacgtgt agaaatcctt aaaacacttt accgtggagc cgacctttta
     4741 atttttgatg agccgacagc tgtattgacc cctgcggaaa ttgcagagct cttgaaaatt
     4801 atgaaagcct tggttgaaga aggtaaatct attattttaa ttacgcacaa attggatgaa
     4861 attcgtgcag ttgccgatag tgtaacagtt attcgtcgag gtaaatcaat cgaaacagtt
     4921 gccgttgaag gagcaacaaa ccaagatttg gctgagtgga tggttggacg ttccgtttcc
     4981 tttaaaacag agaaagttga agcgagtcct aaagaagtca ttttgtcaat taaggatttg
     5041 gttgtcaatg aaaaccgtgg tattccagca gttaagggct tgtcgcttga tgttcgagca
     5101 ggtgaggttg ttggtattgc cggtatcgac ggaaacggtc aaagtgagtt gattcaggca
     5161 atcactggtt tacgtaaggt aaaatctggc tcaatcacta tcaaaggtga ggaagtagtt
     5221 ggtaaaactc cacgtaaaat tacagaaatg caagtgagcc acgttcccga agatcgtcat
     5281 cgtgatggac tcgttcttcc gatgactgtt gctgagaact ttgcacttca aacttactat
     5341 aaagaaccga tttcaaaaaa tggtatcttg aactataata tcattaatga aaaagcacgc
     5401 gcgttaatgg aagaatttga cgttcgtggg gcgagtgaac tcgttccaat caaggctctt
     5461 tcaggcggta accagcaaaa agctattatt gcgcgtgaaa tcgatcgcaa cccagacctt
     5521 ttcattgtta gccaaccaac acgcggtttg gatgttggtg ctattgagta tattcgtaag
     5581 cgtctcattg cagaacgtga taagggtaag gctgttcttg ttgtcagctt cgaattggat
     5641 gaaattttgg atgtatcaga tagaattgct gttatccatg atggtgtcat ccatggtatt
     5701 gttgaacctg ctaagaccaa taaacaagag ttaggtgtct tgatggcagg tggacatatt
     5761 gagaagggag aagaacatgc ttaataaata taagaatttg gcccttccat taatggctgt
     5821 ggtcagtggt atcctacttg gagcgattgt catgcttgtt tttggctacg atccaatttg
     5881 gggctatgaa gagttattct attcagcttt tggtaatatc aaatcaattg gtgaaatttt
     5941 ccgtgcaatg gcaccacttg ttttcactgc tctcggtttc gcagtagcta gtcgtgcagg
     6001 tttcttcaac gttggtttgt ctggtcaggc ctatgttggt tgggtttttg ctggatggtt
     6061 tgccttggcg aatcctgact taccacgtcc agttttgatt ttgggaacag tcttgattgc
     6121 tatgttggca ggcggtattg ctggagctat tccaggtatt ctacgtgcct atttgggaac
     6181 aagtgaagtt atcgttacga ttatgatgaa ctacatctta ctctattcat gtaactatat
     6241 cattcgtgat gtatttgctg atagtttgat gaagaataca gactcgacag ttaacgtgtc
     6301 agcaaatgcc tcttaccaaa cagagtggct ccgtgcattg acagataact cacgtatgaa
     6361 tattggtatt ttctttgcca ttattgcagt ttttgtgatt tggttcctat tgaccaaaac
     6421 gactcttggt tttgagattc gctcagtggg gcttaaccca acagcatcgg attatgcggg
     6481 tatgtctgcg aaacgtacga ttattctatc tatgattatt tcaggtgctc ttgcgggtct
     6541 tgggggagct atccaaggtt tgggtacttt ccaaaacgtt tatatccaaa gcggtaactt
     6601 ggatattggt tttaatggta tgtcagtagc gcttttagct tctaattcac cgttaggtat
     6661 tccattggct gcctttctat tcggtgttct ttctgtcggt gccccaggta tggtacgtgc
     6721 acagattcca cctgaattga tcaacgttgt tacggcatca atcattttct ttatcggtgt
     6781 gaaatttatt tttgaacaat tattaaaatc tagaaataaa gcgaaaggag cgaaataaga
     6841 tgaatgtttc aattcttgcc ttactcattt cgcaaatgtt gatttattca gcacccttaa
     6901 tctttaccag tcttggagga gttttctctg aacgcggtgg tattgtaaac gttggtctag
     6961 aaggaatcat ggttatcggt gcctttgctg gtgttgtttt caatatcgaa tttgcaggga
     7021 catttggtaa tgctactcca ttattagcta ttattgtagg aggtcttgct ggtgttgtct
     7081 ttgcagctat tcatgcgatg gcgacgatta atttccgtgc agaccacgtt gtatcaggta
     7141 cggtgttgaa ccttttggct ccagcattgg gagttttcct agtcaaggtt atctataata
     7201 agggtcaaac tgatagtatt actgaatcat ttggtaaatt ttcattccct atcttggcag
     7261 atattccaat cattggtgaa attttcttca aaaatacaag cttgatgggt tatgtagcta
     7321 ttgcaacagc atttcttgct tggttcatcc tttataaaac caagttcggt ttgcgccttc
     7381 gttcagtagg tgaacatccg caagcagcgg atactttggg aatcaatgtc tacgctatgc
     7441 gttatgcagg tgtgttgatt gctggtttcc ttggtggtgt tggtggagct gtaagtgctc
     7501 aatcagtgaa tatcaacttc tctgcaacaa caatcgtagg ttcaggtttc atcgctctag
     7561 cagctgtaat ttttggtaag tggaatccaa ttggcgctat gcttgccagt ctattcttcg
     7621 gtctttcgca aagtttggca gtagtgggtg gacaattacc aggattgaaa gatattccaa
     7681 cggtttatct tcaaattgca ccatatttga ttactgttat tgctctgtct gctttctttg
     7741 gtaaagcagt cgctcctaaa gcagacggta tcaactatat caaatctaaa taaaaaggtc
     7801 cagtggacct ttttatcatg ttctttgctg tacaggaaat gaataattgt cctattcaaa
     7861 ggaagaggag ttatagggac ttttggttga gaatagggag aatagcttgt cactaaggta
     7921 tgaaatgagg ctaattgtga gacgattggt gttaaacatt gcaatgagtt tagatggatt
     7981 tattgcacgt aaggatggaa gctacgattg gattgacgga catggtaccg ataaatacga
     8041 tacggtctca cagtttgata atcaaaaatt ctttagagg
//
LOCUS       DBNFEG010000098         8067 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.098, whole genome shotgun sequence.
ACCESSION   DBNFEG010000098 DBNFEG010000000
VERSION     DBNFEG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.098"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            91..558
                     /locus_tag="AC1PNG_001399"
     CDS             91..558
                     /locus_tag="AC1PNG_001399"
                     /EC_number="3.5.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004132 - dCMP deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HIP6701901.1"
                     /translation="MSTNRLAWDEYFAAQALLIANRATCKRAKVGAVLVKDNKVIATG
                     YNGSVSGTEHCLDQECLMIDGHCARTLHAEVNAILQGAERGIPKGFTAYVTHFPCLNC
                     SKQLLQVGCKRVVYINEYRMDDYAQYLYKEKGCELVHLPLEVVKQAIADTEFI"
     gene            1281..2471
                     /gene="metK"
                     /locus_tag="AC1PNG_001400"
     CDS             1281..2471
                     /gene="metK"
                     /locus_tag="AC1PNG_001400"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIP6701902.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAEDPDAHVAAE
                     TAVYTGSVHVFGEISTTAYVDINRVVRDTIAEIGYTKGEYGFSAESVGVHPSLVEQSP
                     DIARGVNEALETRQDASQDPLDLIGAGDQGLMFGFAVDETPELMPLPISLSHKLVRRL
                     AELRKSGEIAYLRPDAKSQVTVEYDEDNQPVRVDTVVISTQHDPEVSQEQIRQDVIER
                     VIKEIIPAHYLDDQTNYFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAHPVSVRIDTFGTSTVA
                     ESKLEAAVRQIFDLRPAGIIQMLDLNRPIYRQTAAYGHMGRTDIDLPWEKLDKVEALK
                     TAVL"
     gene            2502..3116
                     /locus_tag="AC1PNG_001401"
     CDS             2502..3116
                     /locus_tag="AC1PNG_001401"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIP6701903.1"
                     /translation="MKALIVFAHPRKESFTHALVDRVAEALLENGAEVVIRDLYEIGF
                     DPVLRGEDTIHIEDGKFIRQATVFPADVQVEMDLIAESDLLVYIFPSWWNGMPAIMKG
                     YVDRVFQHGFAYSFESDEPRKLFSTKKAIFFTPTGQPQNADGSLTPIDQAMKSLTSEW
                     LFNSNGAQVIDHIFYGRVPYLTREELELYLVDAREQIQRIFHKL"
     gene            3264..4523
                     /locus_tag="AC1PNG_001402"
     CDS             3264..4523
                     /locus_tag="AC1PNG_001402"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIP6701904.1"
                     /translation="MRKIVINGGKTLKGSVTVSGAKNSVVALIPAIILADGIVTLDGV
                     PAISDVDSLVDIMETMGATVKRDGETLEIDPRGVKDMPMPFGKINSLRASYYFYGSLL
                     GRFGQAVVGLPGGCDLGPRPIDLHLKAFAAMGAETTYEGDYMRLATNGQRIQGAHIFM
                     DVVSVGATINTILAAVKAEGRTVIENAAREPEIIDVATLLNNMGARIRGAGTDIITIE
                     GVDSLKGTRHQVIPDRIEAGTYIALAAAVGEGIRIDNVLYEHLESYIAKLEEMGVRMT
                     ISEDSIFVEKQEKLKAVSVKTSPYPGFATDLQQPITPLLLKAEGTGTIVDTIYEKRVG
                     HVQELANLGAKISTRNNHIAFEGPNQLTGTSVKATDLRAGAAMVIAGLMAQGRTEITN
                     IEFILRGYSNIIDKLTELGADIQLIED"
     gene            4618..5190
                     /locus_tag="AC1PNG_001403"
     CDS             4618..5190
                     /locus_tag="AC1PNG_001403"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6701905.1"
                     /translation="MTLWTRLAKFAYFETDRLYLRPFSFEDGESFYEIVANPENLPFI
                     FPALEDRNIAFSTMVEKFMRSPLGNWALVDKYSERMIGALCFEKVDERQLSAELSYFL
                     KKDYWRRGLMTEAVRTLVYLAFYEFGLRELVIVTHEENVASQMVAKKAGLVQVAQYRG
                     SDRYSHKTRNYLKFSLKRAEFSLEMYEENE"
     gene            5187..6047
                     /locus_tag="AC1PNG_001404"
     CDS             5187..6047
                     /locus_tag="AC1PNG_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HIP6701906.1"
                     /translation="MITIKSQREIDAMNRAGDVLAGIHIGLREIIKPGVDMWEVEEYV
                     RKTCKEKNVLPLQIGVDGELMDYPYATCCGLNDEVAHAFPRHYKLKEGDLLKVDMVLS
                     EPLDKAVVDVSKLNFNDVKAMKKITQTYRGGCADSCWAYAIGQVSEEVQNLMDVTKEC
                     LYRGIEQAVVGNRIGDIGAAIQEYAEGLGYGVVRDLVGHGVGPTFHEEPMVPHYGTKG
                     RGLRLREGMVLTIEPMINTGTWEIDTDMKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            6095..7024
                     /locus_tag="AC1PNG_001405"
     CDS             6095..7024
                     /locus_tag="AC1PNG_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIP6701907.1"
                     /translation="MKKTKIVEGLKTFCSTFFSFYRSADSDVTSVAVAYYLLISIFPI
                     LLTLANLLPYYPFDVDMILSVVAEFVPDKLYPSVSSFITSVLTKPSSSWLGISILTTL
                     WTLSRSMTILQKAFNKAYGINEHRDFIIGHLIGIFLGIGLQVIILLSITLLTFGQAVF
                     SYINKLVPIEDAWLKGLLSQTQLVGLMALFAALVMLYFFLPNVRIKKVRYVFPGTLFV
                     LLTMTSIGKLFSIYVDNYANKLLDFRIVTAVVFLVFMLWFIFMAQVLIIGAMINATVQ
                     SMQVEEFHARDGDIVSILNRLKARFTAIDKEWE"
     gene            complement(7184..7621)
                     /locus_tag="AC1PNG_001406"
     CDS             complement(7184..7621)
                     /locus_tag="AC1PNG_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIP6701908.1"
                     /translation="MKKLFYQLINNEVILYLIAGVAATLVYMVTRMGLFLVIPSILLV
                     TLLANAVAILFAFWTNDRFVFKQAREGWPQRLVKFVSARIATLVMDMALAYLLVQAYP
                     QLIGQFVNHDLTLVNAISTLFSQVLVIVLNYVLSKLFVFKDKK"
     regulatory      7650..7752
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            7750..>8067
                     /locus_tag="AC1PNG_001407"
     CDS             7750..>8067
                     /locus_tag="AC1PNG_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HIP6701909.1"
                     /translation="MNTSKWTARDMAEIALVAAIYVALTLTPPLNAISFGAVQFRLSE
                     MLNFLAFYNRKYIIAVTIGCMISNLIGFGVIDLFVGGFSTLIFVTLGVILFEKYKKDY
                     LFGG"
BASE COUNT         2252 a         1433 c         1921 g         2461 t
ORIGIN      
        1 ttcattcaat gaatcaactt tttatcattt ttaaaagcac aaatagttgg tggtgagcag
       61 tgatttataa aaatatataa aggagaaatc atgtcaacaa atcgtttagc ttgggatgag
      121 tattttgcgg ctcaggcact tttgattgcc aatcgggcga cctgtaagag agccaaggtc
      181 ggtgcggttt tggttaagga caataaggtt atcgcaacag gctacaatgg ttctgtttca
      241 ggaaccgaac attgtttgga tcaggaatgc cttatgattg atggtcattg cgctagaacc
      301 ttgcatgcgg aagtcaatgc cattttacaa ggagcggagc ggggaattcc aaaaggtttt
      361 actgcctatg tgacccattt cccatgtctc aactgctcca agcaattgtt acaggttggt
      421 tgtaagcggg ttgtttatat caatgaatac cgcatggatg actatgctca gtacttgtat
      481 aaagaaaagg gctgtgagtt ggttcatttg cctctagagg tggttaaaca ggcaattgca
      541 gataccgaat ttatctaatg attttgtaga agagtgggag tgagaaagaa ctcgaccatt
      601 catagaagag ttcgtcaaca atttttcgtt ttaagttgtt gagctgaaac agtctatcct
      661 cagactgttt cactcccacc cccgcatagc tccaacagtc agagtagtga ctgttggagg
      721 ttggaaatga agcgaactct gttcgcatca gtcgtaatgg tcagatttgg agtgtaaaac
      781 acgaactgct gagatttgct tcgcaaatct tatctccaac ctttaacagt ccactagact
      841 gttaaaccca atcaaccact gcgctgagat gttgacacga actctgagta gtggtcctgg
      901 gctttttgcc cagcctcctc tttattttta agaaaatgct aggatagtcg gtcaatctat
      961 gctatactag aaacgttatt aagtcccgaa aaggtagttt atagactagt taatatttgc
     1021 agaaatactt gaaacacaat taaagaagct ggtaacattg aatagtaagc gtaaaaactt
     1081 tactacactt cagtcaccat tttcttcaaa taaggctgat ttcatacagt ctggtattgt
     1141 ctttctcgtt gagtatctgg aaatttccct ttgcttaaga caggtgtctt tcgcaaagaa
     1201 ctagtcttcg actagttctt tgtaacctta gtaacccgct aggtttctag catatattct
     1261 attttaaggg ggacattttt atgtcagaac gtaagttgtt tacgtctgaa tctgtttcgg
     1321 agggtcatcc ggataagatt gcagaccaga tttcagatgc cattttggac gctattttag
     1381 cagaggatcc agatgcgcac gtagcggcag agacggctgt ttatacaggt agtgttcatg
     1441 tgtttggaga gatttcaact actgcctatg tggatattaa ccgtgtggta cgggatacca
     1501 ttgcggagat tggctataca aaaggtgagt atggtttttc agctgagtcg gttggggttc
     1561 atccgtcact tgtagagcaa tcgccagata ttgcccgagg cgtcaatgaa gctttagaaa
     1621 ctcgtcaaga tgctagtcaa gatccattgg atttgattgg tgcaggtgac caggggctca
     1681 tgtttggttt tgcagtagat gaaacgcctg aacttatgcc gttgccaatt tcacttagtc
     1741 acaaattggt gcgtcgtttg gccgagttga gaaaatctgg tgaaattgct tatctccgtc
     1801 cagatgctaa gtcacaggta acagttgaat acgatgaaga caatcaacct gttcgtgtgg
     1861 atacagtcgt tatttcgacc cagcatgatc cagaagttag ccaggagcaa attcgtcagg
     1921 atgtcattga acgtgtgatc aaggaaatca ttccagctca ttatctggat gaccaaacca
     1981 actactttat caacccaact ggtcgctttg tcattggcgg acctcaaggg gactctggtt
     2041 tgacaggccg taagattatc gttgatacct atggtggtta ttctcgtcat ggcggcggtg
     2101 ccttctctgg taaggatgcg accaaagtgg accgttctgc atcttatgcg gcacgctaca
     2161 tcgcaaaaaa tatcgtcgca gctggtttgg ctaaaaaggc ggaagttcaa ttggcttacg
     2221 ctatcggtgt tgctcaccca gtttcagttc gcatcgatac ctttggtacg agcacagtag
     2281 cggaaagcaa actagaagca gctgtccgtc agatttttga cctgcggcca gcaggtatta
     2341 tccagatgct ggacctcaat cgcccgattt acagacaaac tgcggcctat ggccacatgg
     2401 gccggaccga cattgaccta ccttgggaaa aattagacaa ggtagaggcg ttgaaaactg
     2461 cggttttgta atgaaatagt tgtccattta gtgagaagtt tatgaaagcg ttaattgtct
     2521 ttgcacaccc tagaaaagaa agctttacac atgctttggt tgatagagtg gcggaagctc
     2581 tgctggaaaa tggagcagaa gttgtcatcc gagatttata cgaaatagga ttcgatcccg
     2641 tgttacgtgg agaagatacc attcatattg aagatggcaa atttatacgt caggcaacgg
     2701 tttttccagc agatgttcaa gttgaaatgg atttgattgc cgaaagcgac ttactagtct
     2761 acatatttcc gagttggtgg aatggaatgc ctgctattat gaaagggtac gtcgaccgtg
     2821 tatttcaaca tggctttgct tacagttttg aatctgatga accacgtaaa ttattttcca
     2881 ctaagaaagc tattttcttt acgccaacag ggcagccaca aaatgcagat ggaagtctga
     2941 caccgattga tcaagcgatg aaaagcttga cctcagaatg gctcttcaat agcaacggtg
     3001 ctcaagtcat tgaccatatc ttctatgggc gagtacctta tttaacgaga gaagagctgg
     3061 agttgtatct cgtggatgca agggagcaaa ttcagagaat ttttcataaa ttatgaagag
     3121 agattcacta atagtatgta aatatgaagg actgagctca ttggctcggt ccttttgcat
     3181 gctatataac tagctttttc caagacttta tgatagaatg aaacgtatgc ctttttaggc
     3241 aaattaagaa agtgaatgtt agtatgagaa aaattgtaat taatggtgga aaaacactga
     3301 aagggtcggt taccgttagt ggtgcaaaaa actcagttgt tgctcttatt ccggccatta
     3361 ttcttgcaga cgggattgtt actctggacg gagtacctgc aatctcggat gtagatagct
     3421 tagttgatat tatggaaaca atgggcgcga cggtcaagcg tgatggtgaa accttggaga
     3481 ttgaccctcg tggtgtaaaa gacatgccta tgccatttgg caaaattaat agcttgcgtg
     3541 catcctacta tttttacggt tccctccttg gtcgctttgg tcaagctgtt gttggcttgc
     3601 caggtggttg tgatttggga ccgcgtccga ttgatttgca cttgaaagca ttcgcggcaa
     3661 tgggagctga gacaacttat gaaggtgact atatgcgctt ggcaactaat ggtcagcgta
     3721 ttcaaggggc ccatatcttc atggatgttg tcagcgtcgg tgcgacaatt aatactatct
     3781 tagcagctgt caaagcagaa ggccgtacag ttattgaaaa tgcggcgcgt gagcctgaaa
     3841 ttattgatgt ggcaacgttg cttaataata tgggggcacg tatccgtggt gcgggtacag
     3901 atattatcac cattgaaggc gtagacagtc taaaaggaac acgtcatcaa gtgattccag
     3961 accgtattga agcagggact tatattgccc ttgctgctgc tgttggtgaa ggtattcgta
     4021 tcgataatgt cctttatgaa cacttagaga gttacatcgc taagttggaa gaaatgggtg
     4081 ttcgcatgac catttctgaa gatagtattt ttgttgaaaa gcaagagaaa ttgaaggcgg
     4141 taagtgtgaa aacttcacct tatccaggtt ttgcgacaga cttgcagcaa cccattacac
     4201 ctttattgct taaggcagaa gggactggta cgattgttga tacgatttat gaaaaacgtg
     4261 ttggtcatgt gcaggagctt gctaatcttg gagcaaaaat ctctactcgt aacaaccata
     4321 ttgcctttga aggtccaaat caactgactg gtacatctgt taaagctacc gatttgcgtg
     4381 cgggagccgc aatggttatt gctggtttaa tggctcaagg gcgtacagaa attacaaata
     4441 ttgagtttat tctacgtggt tactcaaata ttattgataa actgacagaa ttgggtgcag
     4501 atattcagtt gattgaggat tagagagtct aattgagaac tactttctca aaacgtaaac
     4561 gatttaaaaa caaaaaaatc tggggctggg agtaattctc agccccttgg gtacactatg
     4621 acactttgga caagactagc gaagtttgct tactttgaaa cggaccgttt gtatttacga
     4681 ccattttctt ttgaagatgg agaaagtttc tatgaaattg tagcaaatcc cgaaaatctt
     4741 ccttttatct ttccagcttt ggaggacaga aatattgctt tttcgacaat ggttgaaaaa
     4801 tttatgcgtt caccattggg aaactgggcc ttagttgata agtattcgga gaggatgatt
     4861 ggtgcgcttt gctttgaaaa ggtagatgaa cgtcaacttt ctgcggagct cagctatttc
     4921 ctaaaaaaag actactggag aagaggctta atgacggaag cagtgaggac tttagtctat
     4981 ctagcttttt atgagtttgg tctgagggag ctggttattg tcactcatga ggaaaatgtg
     5041 gccagtcaga tggtggcgaa aaaagctggt ttggtacagg ttgcgcagta tcgtggaagt
     5101 gaccgctata gccataaaac aagaaattat ctaaaattta gcttgaagag ggctgaattt
     5161 agtttagaaa tgtatgagga gaatgaatga ttaccattaa atcacaacga gaaattgatg
     5221 caatgaaccg tgctggagat gttctggctg gtattcatat tggtttgcga gagattatca
     5281 agccaggtgt ggacatgtgg gaagtagagg aatatgtccg taaaacgtgt aaggaaaaga
     5341 atgttttgcc actccaaatt ggtgttgatg gagaattgat ggattatcct tatgcgactt
     5401 gttgtggatt gaatgacgag gtcgctcatg cctttccgcg tcattacaaa cttaaagaag
     5461 gtgatttact gaaggtcgat atggttttga gcgagccttt ggataaagcg gtagttgatg
     5521 tttctaagct caactttaat gatgtcaagg ctatgaagaa aatcacccag acctatcgtg
     5581 gtggttgtgc ggattcctgc tgggcttatg cgattggtca ggtctctgag gaagttcaaa
     5641 acttgatgga cgtgactaag gaatgtttgt accgcggtat cgagcaagca gttgttggca
     5701 atcgtatcgg cgatatcggt gcagccattc aagaatatgc agaaggtttg ggctacggtg
     5761 ttgttcgtga cttggtgggt cacggcgttg gaccaacctt ccacgaagag ccgatggttc
     5821 ctcattatgg aactaaaggt cgtggtcttc gcttgcgtga gggcatggtt ttgactattg
     5881 agccaatgat taatacaggg acttgggaaa ttgatacgga tatgaagact ggttgggctc
     5941 ataagacact tgatggtggc ttgtcttgtc aatacgaaca ccaatttgtc atcaccaaag
     6001 atggaccggt tattttgacc agtcaaggtg aagaagggac ctattaattc tcggtgtggt
     6061 cgtgaataat agatgaatga ggaaggagga gagaatgaag aaaaccaaaa tagtcgaagg
     6121 tttgaaaaca ttctgctcaa ccttcttttc tttttatcgt tctgctgata gcgatgtaac
     6181 aagcgttgca gtagcttact atttattaat ttctattttc cctattcttc taactcttgc
     6241 gaatttactg ccatattatc catttgatgt ggatatgatt ttatcggttg tagctgaatt
     6301 tgtgccagac aagctctatc cttcggtttc atcttttatc acttctgtat taacaaagcc
     6361 gtcgtcatcg tggttaggga tttcaattct gacaactctt tggaccctgt cacgtagtat
     6421 gactattttg cagaaggcct ttaataaggc ttatggtatc aatgaacacc gtgattttat
     6481 tattgggcac ttgattggca tatttttagg aattggtctg caggtaatta tcctcttaag
     6541 tattacgctt ttgactttcg ggcaggcagt tttttcttat attaataagc tggttcctat
     6601 tgaagacgcc tggctgaagg gacttcttag tcaaacacag ttagtaggtt taatggctct
     6661 gtttgccgcc ttggttatgc tttatttctt tttgccaaat gttcggataa aaaaagttcg
     6721 atatgttttt ccaggaacgt tgtttgttct gttgacgatg acttctattg gaaagttatt
     6781 ttcgatttat gttgataatt atgctaataa gttgctggat tttagaatag taacagctgt
     6841 cgtctttctt gtttttatgc tttggttcat ttttatggct caggtactca tcattggtgc
     6901 aatgattaat gctacggttc aaagtatgca ggtagaagag tttcatgcca gagatgggga
     6961 tattgtttca atcttgaata ggctaaaggc aagatttaca gcgatagata aagaatggga
     7021 gtgagaaaga actctatttt caaaaatgag ttcgtcttct cacccccgca cagttgatta
     7081 ggccagattt ggagtgtgaa acgcgaacaa atctgccaat caaccactgc gctgagatgt
     7141 tgacacgaac tctgagaagc gaggttggtc tttttgccca accttatttc ttatctttaa
     7201 atacaaataa tttacttaaa acgtagttta ggacaatgac caagacttga gagaagagag
     7261 tggaaatggc gttgacaagt gtcaaatcat ggttgacaaa ctgcccaatc aattgtgggt
     7321 aggcttgtac taataaatag gctagagcca tatccatgac caaggtcgcg atgcgggcag
     7381 atacaaactt gaccaatcgc tgaggccaac cctctctggc ttgtttgaaa acgaaacggt
     7441 cgttggtcca aaaggcaaag aggatagcta ctgcattggc taaaagagta accaatagaa
     7501 tggaaggaat gactaaaaat agccccattc gagtgaccat atagacaagg gttgctgcca
     7561 cacctgcaat caaatagaga ataacttcgt tattgatgag ttgataaaat agttttttca
     7621 taggactagt ctatcatttt tagcaaaaat atgctatact aatcaacgtt gttgacaaat
     7681 tcaatgaccc ttgggacttg ctcactttac ccaagcatat tttaagcttt tttgctaaag
     7741 gagaatttta tgaatacatc taaatggact gccagagata tggcagaaat tgctcttgtt
     7801 gcggcgattt atgtcgctct taccttgaca ccgccattga atgctatttc cttcggggct
     7861 gttcagtttc gtttgtctga aatgctcaat ttcttggctt tttataatcg taagtatatt
     7921 atcgctgtga ccattggctg catgatttcc aacttgattg gttttggtgt gattgacctc
     7981 tttgtgggtg gattctcgac attaattttc gttacgttgg gagttatcct gtttgaaaaa
     8041 tacaagaagg actatctatt cggtgga
//
LOCUS       DBNFEG010000099         8032 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.099, whole genome shotgun sequence.
ACCESSION   DBNFEG010000099 DBNFEG010000000
VERSION     DBNFEG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.099"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..723
                     /locus_tag="AC1PNG_001408"
     CDS             <1..723
                     /locus_tag="AC1PNG_001408"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIP6701910.1"
                     /translation="VANGFNIPMCEEILEAIRLAEGDASVKILQIQAQGSIFSVGGDL
                     VEMKRAVDDDDIASLVKIAELVNDISFAMKKLSKVVIMVTDGAVAGAAANMAVAADFV
                     IASSKTKFIQAFVGVGLAPDAGGLFLLSRAIGANRASHLAMTGEGLSADKALEYGIVY
                     KLAEPEKLEKTVAQVIKKLLRGSVNSYAAIKQLTWESQFKDWESYRRLELDLQETLAY
                     KEDFKEGVRAHAERRRPVFTGD"
     gene            803..1237
                     /locus_tag="AC1PNG_001409"
     CDS             803..1237
                     /locus_tag="AC1PNG_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIP6701911.1"
                     /translation="MEDPKVNEYLTAIFNNVLVIEETSLRGSRFNDISIKEMHTIDVI
                     GSIDGATPSDVARTLMVTLGTVTTSLNNLERKGYVERIRSTKDRRVVHLYLTKKGRLV
                     YRLHQRFHNEMVKQITDGMDETEYQVMKKGLMKLYHFLEDLK"
     gene            1234..2211
                     /locus_tag="AC1PNG_001410"
     CDS             1234..2211
                     /locus_tag="AC1PNG_001410"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIP6701912.1"
                     /translation="MRNHAKISQVAHYLPKKIVTNDDLAQRMETSDEWIRSRTGIGQR
                     HIVTGETTSDLASQVARKLLEKSQLDASEIDFIIVATITPDASMPSTAAMVQAAIGAK
                     KAFAYDLVAACSGFVFALSTAEKFLASGVYKRGLVIGAETLSRSVDWSDRSTAVLFGD
                     GAGGVLLEACEQPTFLAEILRTDGGRGASLTAGIDQKETPFSTQSRQQPFIQMEGRAI
                     FEFATRDVTATMAELLEQADMTVDRVDYFLLHQANIRILDKMARKLGVVREKFPANMD
                     KYGNTSAASLPILLSECVESGMLRLDGSQTVLMAGFGGGLTWGTLLLQL"
     gene            2271..2495
                     /locus_tag="AC1PNG_001411"
     CDS             2271..2495
                     /locus_tag="AC1PNG_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIP6701913.1"
                     /translation="MAVFEKVQEIIVEELGKDAEEVTLTTTFEDLDADSLDLFQVISE
                     IEDAFDIQIDTEEGLTTVGDLVAYVEEKTK"
     gene            2666..3631
                     /gene="fabK"
                     /locus_tag="AC1PNG_001412"
     CDS             2666..3631
                     /gene="fabK"
                     /locus_tag="AC1PNG_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HIP6701914.1"
                     /translation="MKTKITELLDIKYPIFQGGMAWVADGDLAGAVSNAGGLGIIGGG
                     NAPKEVVKANIDKVKSITDKPFGVNIMLLSPFADDIVDLVIEEGVKVVTTGAGNPGKY
                     MERLHAAGITVIPVVPSVALAKRMEKLGVDAVIAEGMEAGGHIGKLTTMTLVRQVVEA
                     VSIPVIAAGGIADGAGAAAAFMLGAEAVQVGTRFVVATESNAHQAYKEKVLKAKDIDT
                     TVSASIVGHPVRAIKNKLSSAYAAAEKDFLAGKISADAIEELGAGALRNAVVDGDVTN
                     GSVMAGQIAGLVSKEESCEDILKDIYYGAAKVIREEASRWASVGE"
     gene            3633..4553
                     /gene="fabD"
                     /locus_tag="AC1PNG_001413"
     CDS             3633..4553
                     /gene="fabD"
                     /locus_tag="AC1PNG_001413"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIP6701915.1"
                     /translation="MTKIAFLFAGQGSQTVGMARDLYEYYPIVRETFDQASQILGYDI
                     RDLIDKQEDKLHQTRYTQPAILTTSVAIFRLLEEEGIQPDMVAGLSLGEYSALVASGA
                     LAFEDAIALIAKRGEYMETAAPAGTGKMVAVLNTDVNLIEEICSTVTSGIVSPANYNT
                     PAQIVIGGEVAAVDEAVELLKEAGVKRMIPLNVSGPFHTALLRPASEQLAQALEQVEF
                     ADFQVELVGNTEAKVMKKEDIKSLLTRQVMEPVRFYESIATMQEAGVTNFIEIGPGKV
                     LSGFVKKIDKTAHVVAIEDIEGLQIVLNKS"
     gene            4563..5297
                     /gene="fabG"
                     /locus_tag="AC1PNG_001414"
     CDS             4563..5297
                     /gene="fabG"
                     /locus_tag="AC1PNG_001414"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011225529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIP6701916.1"
                     /translation="MELTNKNVFVTGSSRGIGLAIAHKFASLGANVVLNGRGQLGQDL
                     LDSFADYGVKVLAISGDISSAEDAKRMVAEAIETLGSVDILVNNAGITKDGMALRMTE
                     EDFDTVLKVNLTGTFNMTQAVLKPMTKAREGAIINLSSVSGLIGNAGQANYSASKAGV
                     IGFTKAIAREVAGRNVRVNAIAPGFIQSDMTDVLSDKIKEAMTAQIPMKRFGATEEVA
                     DVAVFLAKQEYLTGQVIAVDGGLTMR"
     gene            5691..6926
                     /gene="fabF"
                     /locus_tag="AC1PNG_001415"
     CDS             5691..6926
                     /gene="fabF"
                     /locus_tag="AC1PNG_001415"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIP6701917.1"
                     /translation="MTKLNRVVVTGYGLTSPIGNTPEEFWDSLKAGKIGIGKITKFDT
                     SEYSVHNAAELKDFPFDKYFVKKDTNRYDNYSLYALYAAKEAIANAQLDTETVDSDRF
                     GVILSTGIGGILEIEEQVARMNEKGPKRIRPMALPKALPNMAAGNIAMQVGANGVCKC
                     IITACASSNDALGEAFREIKFGFQDVMLAGGAEAAITPFAIGGFQALTAMSTTEDPER
                     ASIPFDKDRNGFVMGEGSAVLVLESLEHAEARGATILAEIVGYGNTCDAYHMTSPHPE
                     GLGAIKAMKLAISEAGLEPADIDYINAHGTSTPANEKGESQAIVSVFGKNTPVSSTKS
                     FTGHLLGAAGAVEAAAVIEAMRHSYAPKTAGTTELSEDIEADVIYGQGRDMEIRHAIS
                     NTFGFGGHNSVIAFKRWEA"
     gene            6927..7403
                     /gene="accB"
                     /locus_tag="AC1PNG_001416"
     CDS             6927..7403
                     /gene="accB"
                     /locus_tag="AC1PNG_001416"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIP6701918.1"
                     /translation="MNITEIKDLMSQFDQSSLREFSYSNAGETLHFSKNQQATVAPSP
                     AVEAPLAPVTPASPALVPAVEMSEPAPAEAGASPVAEGAVVESPLVGVAYLSPAPDKP
                     AFVAVGDTVKKGQTLMIIEAMKVMNEVPADRDGVVTEILVANQDVVEFGQGLVRIK"
     gene            7400..7822
                     /gene="fabZ"
                     /locus_tag="AC1PNG_001417"
     CDS             7400..7822
                     /gene="fabZ"
                     /locus_tag="AC1PNG_001417"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIP6701919.1"
                     /translation="MIDIVKIKEALPHRYPMLLVDRVLEVSEDEIVALKNVTINEPFF
                     NGHFPDYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     IMTATFVKRRGTIAVVEAKAEVDGKLAASGVLTFAIGK"
BASE COUNT         2288 a         1511 c         2045 g         2188 t
ORIGIN      
        1 gtggcaaatg gttttaatat tcctatgtgt gaagaaatct tagaagcaat ccgccttgca
       61 gaaggggatg cctccgtcaa aattttacaa attcaagcac agggctccat tttctccgtg
      121 ggtggagatt tagttgagat gaagcgtgca gttgatgatg atgatattgc ttctttggta
      181 aaaatagcag agctggtgaa tgatatttcg tttgcgatga agaaattgtc taaagtagtc
      241 attatggtga cagatggggc agtggctgga gccgcggcta atatggcagt ggcagctgat
      301 tttgttattg cctccagcaa gacaaaattc atccaagcct ttgtaggtgt tggacttgcg
      361 ccggatgcgg gggggctctt tctactgagt cgtgctattg gggctaatcg tgctagtcac
      421 ttggcgatga caggagaggg cttatcagcg gataaggctt tagaatatgg gattgtttat
      481 aaattggcag agccagaaaa actagaaaag acggttgccc aggtcataaa aaaactcttg
      541 cgaggctcag taaactctta tgcagcaatc aaacaactga catgggaaag tcaatttaaa
      601 gactgggagt cttatcgccg attggagctg gatttgcaag aaacattggc ctataaagag
      661 gattttaaag agggagttcg agcgcatgca gaacgacgta gaccagtgtt tacgggagat
      721 taatttttaa attagtaaaa aaagtttgac aatcaaagtt ttatggttta tactttgact
      781 atcaaagtaa aggaggcctg ttttggaaga tccaaaagta aatgaatatc taacggccat
      841 ttttaacaat gtgttggtga ttgaagaaac aagtctgcgt ggtagccgat tcaatgatat
      901 ttcaattaag gaaatgcaca cgattgatgt gataggaagt attgatggag ctactccgag
      961 tgatgttgct cggactttga tggtaacctt aggtactgtt acaactagct tgaacaattt
     1021 ggaaagaaaa ggatatgttg agcgtattcg ttcgaccaaa gatcggcgag ttgttcatct
     1081 ctatctgacc aaaaaaggtc gactggtcta tcgtttgcat caacgatttc acaatgaaat
     1141 ggtgaaacag attacagatg gtatggatga aacagagtat caggtaatga agaaaggact
     1201 catgaaactc tatcacttct tggaggattt gaaatgagaa accatgctaa aatcagtcag
     1261 gtggcccact atcttccgaa gaagattgtg accaatgatg atttggctca acgaatggag
     1321 accagtgatg agtggattcg tagccgtaca ggtatcggtc aacgacacat tgttactgga
     1381 gagacgacga gtgatttggc tagtcaagtg gctagaaaat tactggaaaa gtctcagcta
     1441 gatgctagtg aaattgattt catcatcgta gcaacgatta ctccagatgc ttcaatgcca
     1501 tctacggcag ctatggtcca agcagcaatt ggtgcgaaaa aggctttcgc ctatgatctt
     1561 gttgcagctt gttcaggatt tgtttttgcg ctatcaacag cggaaaaatt ccttgcttca
     1621 ggagtctata agagaggact tgtcattggt gcagagaccc tatctaggtc tgtcgactgg
     1681 tcggatcgat caacggctgt tttatttgga gatggtgctg gaggagtttt gctggaagcg
     1741 tgcgaacaac cgaccttttt agcagaaatc ttgcgaacgg atggcggtcg aggtgcgagt
     1801 ctgacagcag ggatagacca gaaagagacg ccattctcga cacaaagccg tcaacaaccc
     1861 tttatccaaa tggagggcag agctatattt gaatttgcga cgagggatgt tacggcaacc
     1921 atggcagagc tgctagaaca agctgatatg actgtggatc gtgttgatta tttcttgctc
     1981 catcaagcca atatacgtat cctagataag atggctcgta aattgggagt ggtgcgtgaa
     2041 aaatttccag caaatatgga taaatatggc aataccagcg cagcaagtct tcctattctc
     2101 ttatcagaat gtgtagagag cggaatgtta cgattggatg gaagccagac tgttctcatg
     2161 gctggtttcg gtggaggcct cacatgggga actctactat tacaacttta gttaattcct
     2221 tgccctaggc aagattaaaa tattttattt tccaaaagga gacacatatc atggcagtat
     2281 ttgaaaaagt acaggaaatc atcgttgaag aattgggtaa ggatgctgaa gaagtaaccc
     2341 ttacaacaac ctttgaagat ttggacgcag attcattgga tcttttccaa gttatctctg
     2401 aaatcgaaga tgcattcgac atccaaatcg acactgaaga aggtttgact actgtaggtg
     2461 atttggttgc ctacgttgaa gaaaaaacaa aataatgact gttaaggaaa gtgctattta
     2521 gcatttttct taaattaaat actttgataa tcaaaacatt gaaatatcaa actatttttt
     2581 gagtgagggg gcaacccttc acttgtttac ctaatgattt gatattcaaa tgattaggta
     2641 agcaagaaaa aaagatgagg gaattatgaa gacgaaaata acagaattgc tcgatattaa
     2701 gtatccaatt tttcaaggtg gtatggcttg ggtagctgat ggagatttgg ctggtgccgt
     2761 atctaatgca ggtggtctag gaatcatcgg tggaggaaat gctcccaaag aggtagtcaa
     2821 ggcaaatatt gacaaggtta aatctatcac ggacaaacct ttcggtgtca atatcatgct
     2881 gttgtctcct tttgcagatg acattgttga cttggtcatc gaagaaggtg tgaaagttgt
     2941 cactacagga gcaggaaatc cagggaagta tatggagcgt ctccatgctg cagggattac
     3001 agttattcct gttgtaccga gtgtagccct tgctaaacgc atggaaaaat taggggtaga
     3061 tgctgttatt gctgaaggta tggaagccgg aggccatatt ggtaaattga ccaccatgac
     3121 cttggttcgt caggttgttg aagccgtatc tattcctgtt attgctgctg gaggtattgc
     3181 agatggtgca ggtgctgcgg cagcctttat gttgggagca gaagcggtgc aagttggaac
     3241 tcgttttgta gtagctacag agtccaatgc ccatcaggct tataaggaaa aagttttaaa
     3301 agctaaggat attgatacaa ctgtatctgc ttctattgtt ggacatcctg tgagagccat
     3361 caagaataag ttgtcatctg cttatgcagc tgcagagaaa gatttcttgg caggtaaaat
     3421 ttcggcagat gctattgaag aacttggtgc gggagcactc cgcaatgcag ttgttgatgg
     3481 tgatgtaaca aatggctccg tgatggcagg tcaaattgct ggtctggttt ctaaagaaga
     3541 atcttgtgaa gatattttga aagacatcta ttatggagca gccaaagtga ttagagaaga
     3601 ggcaagccgt tgggcttcgg ttggagaata ggatgacaaa gatagctttt ctttttgcag
     3661 gtcaagggtc tcagacagtg gggatggctc gcgaccttta cgaatattac ccgatagtta
     3721 gagaaacgtt tgaccaggct agtcagattc tggggtatga cattcgcgac ctaattgaca
     3781 aacaagagga taaattacat cagactcgct atacccaacc ggcaattttg acaacttctg
     3841 tggctatttt tcgactgctg gaagaagagg gaatacaacc cgatatggta gctggtctat
     3901 ccttgggaga atattcagct ctggtagcat cgggagcttt ggcctttgaa gatgcgattg
     3961 ccctgattgc taagcgtggt gaatatatgg aaacggctgc gccagcgggg actggtaaga
     4021 tggttgcggt tctcaacaca gatgtcaact tgattgaaga aatctgttcg actgttacct
     4081 ctgggattgt ttccccagcc aactacaaca caccagccca aatcgtcatc ggaggcgagg
     4141 tggctgcagt ggatgaggcg gttgaattgc taaaggaagc tggtgtcaaa cggatgattc
     4201 cgctcaatgt atctggcccc tttcacacgg ctctcttgcg tccagcttcg gagcaattgg
     4261 ctcaggcctt ggagcaggtg gaatttgcgg acttccaagt agagttggtc ggtaatacgg
     4321 aagccaaggt tatgaaaaaa gaggacatca agtccctctt gacccgtcaa gtgatggaac
     4381 cggttcgttt ctatgagtca atcgcaacca tgcaggaagc tggcgtgact aactttattg
     4441 aaatcggtcc aggtaaagtc ttatcaggtt ttgtcaagaa aatcgataag accgcgcatg
     4501 ttgtagcaat tgaagacata gaggggctac aaatagtatt gaataaatca tgaggaggaa
     4561 tcatggaatt aacaaataaa aatgtttttg taacaggttc aagccgagga attggcttgg
     4621 ccattgccca taagtttgcc agtcttggtg ccaatgtggt gctgaacggt cgtggtcagc
     4681 taggccagga cttgctggat agctttgcag attacggtgt gaaagtccta gctatttcag
     4741 gagatatttc aagcgcggaa gatgccaaac gaatggtggc agaagccatc gaaacactcg
     4801 gtagcgtcga tattttggtc aacaatgcag ggattaccaa ggatggaatg gctcttcgta
     4861 tgacagaaga ggattttgat acggttctga aggtaaatct tactggaact tttaatatga
     4921 cgcaggctgt tttgaaacca atgacaaagg ctcgtgaggg tgctattatc aatctatcaa
     4981 gcgtatctgg cttgattgga aatgctggac aggccaacta ttctgcttct aaggcaggtg
     5041 tgattggatt tactaaagcc attgcccgtg aggttgctgg gcgaaatgtt cgtgtcaatg
     5101 cgattgcgcc gggctttatc cagtctgata tgacagatgt tttatctgac aagattaaag
     5161 aagctatgac tgcacaaatt ccgatgaaac gctttggtgc gacagaagaa gtagcggatg
     5221 tagcagtgtt tcttgccaag caagaatatc taactggaca ggtgattgcg gttgacggcg
     5281 gcttgaccat gcggtaatcc agtcttccag tttatctttt attacttcga cgagtgagga
     5341 aactacgttt ccagatttcc agctgggagt gggaaagaac tcgaccaaga taaaaagagt
     5401 tcgtcttccc acccccgcac agttgattag gccagatttg gagtgcaaaa cacgaacaaa
     5461 tctgccaatc aaccactgcg ctgagatgtt gacacgaact ctgagtagtg gtcctgggct
     5521 ttttgcccag cctcacgatt tccaaccttc aatagtccac tggattattg aagctgtcaa
     5581 caacttgttg aaatgaactt tttcgtagta ctaaaagcaa actgaaagac tgcaataacg
     5641 cactgtaaat tcaatgttgt tttgtgtgtt attttaagag aggtttgaaa atgacaaaat
     5701 taaatcgtgt agtagtaaca ggctacggtc tgacatctcc aatcggaaat acgccagagg
     5761 agttctggga tagtttgaag gctgggaaaa ttgggatcgg aaagattacc aagtttgata
     5821 ccagtgaata ttcggtccat aatgccgcgg aattaaaaga ttttcctttt gacaaatatt
     5881 tcgttaaaaa ggatacaaat cgctacgata attactcgct ctatgcactc tatgcagcta
     5941 aagaagcgat tgctaatgca cagctggata cagagacagt ggatagtgac cgttttggcg
     6001 ttatcttatc aacaggtatc ggtggtatct tggaaattga agagcaagtg gctcgtatga
     6061 atgaaaaagg tccaaaacgc attcgtccca tggctcttcc aaaggccctg ccaaatatgg
     6121 cggctggaaa tattgctatg caggtcggtg caaatggcgt ctgcaagtgc atcatcacag
     6181 cctgcgcttc atcaaatgat gctttagggg aagccttccg tgaaatcaag tttggtttcc
     6241 aagatgtgat gctggctggc ggagcagagg cagccattac tccctttgct atcggtggtt
     6301 tccaggcttt gacagctatg tcgactactg aggatccaga acgtgcgtct attccatttg
     6361 acaaggaccg caatggtttt gtcatgggag agggttccgc ggttttagta ttggaaagtt
     6421 tggaacacgc agaggcgcgt ggagcgacga ttttggctga aatcgttggt tatggaaata
     6481 cctgtgatgc ttaccacatg acttctccac atccagaagg tctgggtgct attaaggcca
     6541 tgaagttggc catttcagaa gcaggtttgg agccagctga tattgactac atcaatgccc
     6601 atgggacttc gacaccggct aatgaaaaag gggaaagtca agctatcgta tctgtcttcg
     6661 gcaagaacac gccagtttct tctaccaagt ccttcactgg tcacttgttg ggtgcagcgg
     6721 gtgccgttga agcggcagct gtcattgagg ctatgcgtca ttcttacgca ccaaagacag
     6781 ctggtacgac agaattatct gaagatattg aagcggatgt catttatgga caggggcgtg
     6841 atatggaaat ccgccatgcc atttcaaata catttggctt tggtgggcat aattcagtca
     6901 tcgctttcaa acgttgggag gcctaagtga atattacaga aattaaggac ctgatgagtc
     6961 agtttgacca gtcaagtttg cgagaatttt cgtatagcaa tgctggggag accttgcatt
     7021 tcagtaaaaa ccagcaggcg acagtggctc cgagtccagc ggtagaagct ccgcttgccc
     7081 cagtaactcc tgcaagccca gcccttgtgc cagcagttga aatgagtgag ccagctccag
     7141 cagaagcagg tgccagcccc gtggcagaag gtgcggtagt ggaaagcccc ttggtcggcg
     7201 tggcttattt gtccccagcc cctgacaagc cagcatttgt agcggtcggc gatacggtca
     7261 aaaaaggcca gaccctcatg attatcgaag ctatgaaagt tatgaacgaa gtaccagctg
     7321 accgtgacgg cgtagttaca gaaattttgg tggccaacca agatgttgta gaatttggac
     7381 aaggattggt acgcatcaaa tgattgatat tgtaaaaatt aaagaggctc ttcctcatcg
     7441 ctaccctatg ctcttggtcg atcgtgtcct tgaagtatcg gaagatgaga ttgtcgccct
     7501 caaaaatgtg accattaacg agcccttctt caacgggcat ttcccagact atcctgttat
     7561 gccaggtgtt ctcatcatgg aggccttggc acaaaccgca ggtgtcttgg agttgtctaa
     7621 ggaagaaaac aagggcaagc tggtttttta tgccggcatg gacaaggtca aatttaagaa
     7681 gcaagttgta cctggtgacc agttgatcat gaccgctaca tttgtcaaac gccgtggaac
     7741 cattgcggtc gttgaagcaa aggcagaggt ggatggaaaa ctggctgcgt cgggggtact
     7801 aacctttgcc attggaaaat aaatcagttc tccattttat cttttattgc gtcgacgaac
     7861 gaggaaactt cgtttcctta tttccaactt caaataattt catcattatt tgaactcgtt
     7921 ttgccgtacc caagtactgt ctacaacaat tgatacgaat tttattcgtc tcatttccaa
     7981 cctccaaagg tttcccaaac ctttggagca agcactaaaa gtaaaatgaa ga
//
LOCUS       DBNFEG010000100         8029 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.100, whole genome shotgun sequence.
ACCESSION   DBNFEG010000100 DBNFEG010000000
VERSION     DBNFEG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8029
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.100"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..568)
                     /locus_tag="AC1PNG_001418"
     CDS             complement(<1..568)
                     /locus_tag="AC1PNG_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701920.1"
                     /translation="MNVAIIILVAIFVILLLLLLVYVALFQIWLKSSRQIHEYDEDII
                     RDYLCKLAESFEYSSTQIPYGRARWFILGSKDFVCTVNDVENLEIFGYSPIRSKIDEE
                     FLEYGLLLTQDGFYFREQIENSNNDADVKYKISKKFFPLKGLWKITCKGKKILLFYPN
                     KIERLNFQFDDAQQKNIVANLNSIIKTGY"
     gene            complement(581..1327)
                     /locus_tag="AC1PNG_001419"
     CDS             complement(581..1327)
                     /locus_tag="AC1PNG_001419"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIP6701921.1"
                     /translation="MATYETFAAVYDEIMDDSLYDKWTDFSLRNLPQDTKTILELACG
                     TGIQAVRFAQKGFEVTGLDLSYEMLELAQKKAEVAGVMMELAQADMMALEGVGDFDAV
                     TCYSDSLCYMADQEAVLRVFEGVHSVLNQGGTFLFDVHSIYQMEEVFAGYSYHENYED
                     FAFVWDTYEGEYAHSIVHELTFFVKDEDSQEERFIRRDEVHEERTYPIETYVELLKEA
                     GFASVEVFADFENQAPTETSQRWFFKCKKK"
     gene            complement(1784..2143)
                     /gene="rsfS"
                     /locus_tag="AC1PNG_001420"
     CDS             complement(1784..2143)
                     /gene="rsfS"
                     /locus_tag="AC1PNG_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIP6701922.1"
                     /translation="MKELDLVKVVVQAADDKRAEDLVVIDVQGVTSLTDYFVIASSMN
                     SRQLEAIAENIREKVAEVGIKSSRVEGDSNGGWVLLDLGGLVVHIFSEEMRDLYNLEK
                     LWHEGSFLEVADFLEEE"
     gene            complement(2145..2654)
                     /locus_tag="AC1PNG_001421"
     CDS             complement(2145..2654)
                     /locus_tag="AC1PNG_001421"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIP6701923.1"
                     /translation="MKTALIVIDIQSMPVEEQPYAIEERLALWQDSLAQARQAGLEVI
                     HVRHHDQELVKGTADWEIHSIVAPLASEKIFDKTFNSAFKETRLHAYLQEKEIERLII
                     MGMATNFCIDTTIKVAFELGYKVAVIQDGTTTGYSGKLDAEDLIDHYQNIWSWNFAQV
                     DKLENIIRG"
     gene            complement(2664..3106)
                     /locus_tag="AC1PNG_001422"
                     /pseudo
     CDS             complement(2664..3106)
                     /locus_tag="AC1PNG_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735474.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3109..3696)
                     /gene="yqeK"
                     /locus_tag="AC1PNG_001423"
     CDS             complement(3109..3696)
                     /gene="yqeK"
                     /locus_tag="AC1PNG_001423"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HIP6701924.1"
                     /translation="MIAADLTFDRQALLEKIRVAMKPARFQHVLGVEEAALALADRYG
                     CDPKKASLAALLHDYAKEVEDQVFLDLIAKYDLDKALLNWDNNIWHGVVGAYKIAEDF
                     GLEDQEIFQAIQRHTIGAGQMTLLDKVLYVADYIEPNRDFPGVDEARRIAKESLDKAV
                     AYETAQTISYLAKKGIPIYPQTLETYNGYVVWLKE"
     gene            complement(3693..4325)
                     /locus_tag="AC1PNG_001424"
     CDS             complement(3693..4325)
                     /locus_tag="AC1PNG_001424"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIP6701925.1"
                     /translation="MAIELLTPFTKVELEVEKKETNRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLKLDEVLLMPEFIPPHVDKKETIDEYHRYSMLKMAIAGIEGLGIETIELERR
                     GVSYTYDTMKLLKEKNPNTDYYFIIGADMVDYLPKWHRIDELVQLVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRSFIKQGRVPNFMVPHEVLDYIEEKGLYQ"
     gene            complement(4338..4649)
                     /gene="yhbY"
                     /locus_tag="AC1PNG_001425"
     CDS             complement(4338..4649)
                     /gene="yhbY"
                     /locus_tag="AC1PNG_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HIP6701926.1"
                     /translation="MMLTSKQRAFLNSQAHSLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKQVKE
                     I"
     gene            complement(4968..6074)
                     /gene="yqeH"
                     /locus_tag="AC1PNG_001426"
     CDS             complement(4968..6074)
                     /gene="yqeH"
                     /locus_tag="AC1PNG_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HIP6701927.1"
                     /translation="MEELFCIGCGAQIQTLDKAVAGFTPQSALEKGLETGQLYCQRCF
                     RLRHYNEISDVNISDDDFLKLLHSVGESDALVVNVIDIFDFNGSVIPGLPRFISGNDV
                     LLVGNKQDILPKSVKTGKVTQWLTERAHEIGMRPVDVVLTSAQNKQAIKDLIEKIEQH
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDKDVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYISPRKEIKPKTYQLNPEQTLFLAGLGRFDFVAGERQ
                     GFTAFFDNELQLHRTKLQGASEFYQKHAGSLLVPPTSKELKEFPELVRHEFTINEKTD
                     VVFSGLGWIRVNEKAKIAAWAPKGVDVVIRKAII"
     gene            complement(6075..6602)
                     /locus_tag="AC1PNG_001427"
     CDS             complement(6075..6602)
                     /locus_tag="AC1PNG_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HIP6701928.1"
                     /translation="MSLENYMPDFALEKAYDVTVESLKKHGIKVVFVDLDNTLIAWNN
                     PDGTPEMRQWLHDLQDAGIPVVVVSNNKYERVKRAVEKFGIEFEAFALKPFTFGINRA
                     LKRFDVQPYEVIMIGDQLMTDIRAAKRAGLKSVLVKPLIKTDSINTQINRWRERRTMK
                     KIIAKYGAIDYKREM"
     gene            complement(6786..7160)
                     /locus_tag="AC1PNG_001428"
     CDS             complement(6786..7160)
                     /locus_tag="AC1PNG_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701929.1"
                     /translation="MNPLRKELRTVAVEVSDLALDYAVRLAQSLNSTLRYHNYDSLIA
                     IAKTKGVEPKGKDCQSFSEYRQRYSLYDAKKLIYRALAWRLFDDSHADYGHALTILGL
                     DEDESGVEQIGFAFSKFTLDID"
     gene            complement(7367..7996)
                     /locus_tag="AC1PNG_001429"
     CDS             complement(7367..7996)
                     /locus_tag="AC1PNG_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HIP6701930.1"
                     /translation="MIKEFCSENHVAVVKALSLGAQRVELCDNLAVGGTTPSYGVIKH
                     VCQLAHENEATVMTMIRPRGGDFCYDEAEVEMMVEDCKAAIELGADGLVYGLLTEENW
                     LDEPALERLFAVSQDCQIVFHMAFDQIPRERQFEALDWLVEHGVTRILTRGSLTGSAL
                     NNVEWLQEIVAYAQGKIEILIGGGLTVDNVPTLLEALPINQVHGTRLFW"
BASE COUNT         2292 a         1977 c         1446 g         2314 t
ORIGIN      
        1 tataacctgt tttgataatt gagtttaaat ttgcaactat atttttttgt tgagcgtcat
       61 caaattgaaa atttagtctc tcaatcttat taggataaaa aagtagaatt tttttaccct
      121 tacaagtaat cttccataaa ccttttaaag ggaaaaactt tttgctaatt ttatacttaa
      181 catcagcatc attattgcta ttttcgattt gttctctaaa ataaaatcca tcctgggtta
      241 acaacaaacc atattccaaa aactcctcgt caattttgct cctaatagga ctatagccaa
      301 aaatctccaa attctctacg tcattaactg tacaaacaaa atctttgctc cccaatataa
      361 accatctagc ccgaccgtag ggaatctgcg tactagaata ttcaaaagac tcggctaatt
      421 tgcataaata atcacgaata atatcttcat cgtattcatg tatttgacga cttgatttta
      481 accatatttg aaacaaagct acataaacta ataataacaa taaaagaata acaaatattg
      541 caaccaagat aatgatagct acattcatat ccaataccac ctatttcttc ttacacttga
      601 aaaaccaccg ctggctagtt tctgtcggag cttgattttc aaagtccgca aagacttcca
      661 cagaggcaaa gccagcttct ttcaataatt ccacataggt ttcaatcgga taggtccgct
      721 cttcatgaac ttcatcccga cgaataaacc gctcctcttg actgtcttca tctttgacaa
      781 agaaggtcaa ttcgtgaaca atcgaatgtg catactcccc ctcataggtg tcccagacaa
      841 aggcaaaatc ctcgtagttt tcatggtagc tatagcctgc aaagacttcc tccatttgat
      901 agatggagtg aacgtcaaaa aggaaagtac caccttgatt gagaacagag tgaactcctt
      961 caaagacccg caaaactgct tcttggtccg ccatgtagca aaggctgtcc gagtaacagg
     1021 tcaccgcatc gaaatctcca acaccttcca gagccatcat atctgcctgg gccaattcca
     1081 tcatgacacc agcaacttct gcttttttct gagccaattc caacatctca taggataggt
     1141 caagccctgt cacttcaaag cccttttgag caaaacgcac agcctgaatc cctgtcccac
     1201 aggccaattc taaaattgtc ttggtgtctt gcggcaagtt ccgcaagcta aaatctgtcc
     1261 atttatcata caagctgtca tccatgattt catcatagac cgccgcaaag gtttcgtaag
     1321 ttgccatatt tcccattttc cctttcataa aaagcagcca atcgactgct tcattaactc
     1381 acactgtgtt caagaagttc atcacttcta attacataat agtgaactct ctttggtcac
     1441 tttcgaattt tatggtgacc agctgaaaca gttccccaaa ctgtttcact cccacccccg
     1501 cacagctcca acagtcagag tagtgactgt tggaggttgg aaatgaagcg aactcgttcg
     1561 catcagccgt ataggtcaga tttggagtgg gaagcacgaa caaagctgcc aatcaaccac
     1621 tgcgctgaga tgttgactca agtttcgaat agtgcaccca aatcaaaatc tatcttcata
     1681 tttttcaaga agttcatcac ttctaatcac ataatagtga actctctttg gtcactttcg
     1741 agttttatgg tgaccagctg aaacaattcc ccaaactgtt tcactattcc tcttctaaaa
     1801 agtctgcgac ttccaggaag ctgccttcat gccataattt ttcaaggttg tagaggtcac
     1861 gcatttcttc tgagaagata tgaacgacta ggcctccaag gtccaaaagg acccaaccac
     1921 cgttactatc gccttctaca cggctactct tgattccaac ttccgctacc ttttcacgga
     1981 tgttttccgc aatagcctcc aactggcgac tgttcatgga actggcaatc acaaagtagt
     2041 ctgtcaggct ggtcacgcct tgaacatcaa ttacaacaag gtcctcagct cgcttgtcat
     2101 cagcagcctg aaccacaact tttactaaat ctagttcttt catcttatcc tctaattata
     2161 ttttctaatt tatccacctg ggcaaaattc catgaccaga tgttttggta gtggtcaatc
     2221 aagtcttcgg catccaattt gcctgaataa cctgtcgtcg ttccgtcctg gatcacagct
     2281 accttgtagc ctagttcaaa ggctacttta atcgtcgtat caatgcaaaa attggtcgcc
     2341 atgcccatga tgatgagtcg ctcaatttcc ttttcctgca aataagcatg aagcctcgtt
     2401 tccttgaagg cactgttaaa ggtcttatcg aaaatcttct cactagctaa gggtgcaaca
     2461 attgagtgaa tctcccagtc agcagtcccc ttgaccaatt cctgatcatg atgtcggaca
     2521 tggatgactt ctagccccgc ttgacgggct tgagcaagac tatcttgcca aagagccagt
     2581 cgttcttcaa ttgcataggg ttgctcctcc acaggcatac tctgaatgtc aatgactatc
     2641 aaggctgttt tcatatccac tccctaaggc gtttccaaac ggaagaccaa ttccctttct
     2701 tcttcactat ccaagacaaa atcatttttc tccaagagat gagccatctt ctgattgccc
     2761 ttaacataag ctgcaatcaa atggtcgcag acaggatgtg attgagccat tgctattaca
     2821 acctccaaag cagcttgacc atagcccctt ccttgaaact cttggcaatc ataaaccgcc
     2881 agagcatata ctgctggatg tcctcatcaa tgtcaatcaa ggcaaaaccg accacctgtt
     2941 gacccgccca aacagcgtaa gggaagacat ccccgttttc tctgtaaagc caagcgtcag
     3001 cgagtgaacg cacatttgga gccacgaagc ccttgtcctc ctcggctact gtcaagtcca
     3061 agactgcttg ataggagctt tcatccacag gttttaatgc aatcatccct actccttcaa
     3121 ccaaacaaca tagccattat aggtttccaa ggtttgtgga taaatgggaa ttcccttctt
     3181 ggctaagtaa gaaatggttt gggcagtttc ataggctact gccttgtcca aggactcttt
     3241 tgcgatacga cgcgcctcgt ccaccccagg aaaatcccga ttgggttcga tatagtccgc
     3301 cacatagaga accttgtcca gcaaggtcat ctgcccagca ccaatggtgt gtcgttgaat
     3361 ggcttggaag atttcttggt cttccaaacc aaaatcttct gcgattttat aggccccaac
     3421 cacaccgtgc cagatgttat tatcccagtt gagcagggcc ttgtccaaat catacttggc
     3481 aatcaagtcc aaaaaaactt ggtcttccac ttccttggca tagtcatgca agagtgctgc
     3541 cagactggct tttttcggat cacagccgta tcgatctgcc aaggcaagcg ccgcctcttc
     3601 cacacccaag acatgttgga agcgcgctgg cttcatggct acacggattt tttccaaaag
     3661 agcttgacgg tcaaaggtca aatctgctgc aatcattggt aaagtccttt ttcttcgata
     3721 tagtccagaa cttcatgagg caccataaaa ttcggcaccc gtccttgctt gataaagctc
     3781 cgtaccatgc tggaggaaat gtccatcaag ggcacatcta cccagataac cggataggac
     3841 gtccctgcct tgtaacgagg acgctggaca ccaacaaact gcaccaactg gaccaattca
     3901 tcaatcctgt gccattttgg aaggtagtct accatatctg caccgatgat gaaataataa
     3961 tccgtattag gatttttttc cttcagcaat ttcattgtgt cgtaggtata gctgacacca
     4021 cggcgttcta gttcaatggt ttcaatcccc aagccctcaa tacctgcaat agccatcttg
     4081 agcatggaat agcgatggta ttcgtcaatg gtttcttttt tatccacatg aggcggaatg
     4141 aattctggca tgagcaagac ttcatccagc ttcaactgtt gacggacctg gtctgccaca
     4201 atcaaatgag cattatggac agggttgaaa ttccctccca gaatccccac ttgtttgcga
     4261 ttggtttctt tcttttcaac ctctaattca accttggtaa agggtgttaa gagttcaata
     4321 gccataggct ctccttctta gatttctttg acctgtttag aaattttacg gttctctttc
     4381 ttgctagatt gtttgaataa aatcaagatg cgaccgattt tttggaccgt atccacaccg
     4441 atttcttctt ccaagatttc tgctacttcg tgaatgtttt catccgtatt ttgtagcaag
     4501 gtcaccttaa tcaattcacg cgcatccaac gcctgacgaa cactggtttt aatctggtca
     4561 tttagaccat ttttcccaat ctgaataatg ggtttgagac tatgtgcctg actgttcaaa
     4621 aaggcacgtt gttttgaagt tagcataatt tattatacaa agggcgttgt aacagactgg
     4681 gagactgtta caatcgggaa ataaagaaaa caaagttttt ccttattgcg ccgatatact
     4741 tagcccgtgt tcaaatcaga agacttgaac ttcctttctc tttaattgta aaaactgaaa
     4801 tcaaatacct cttatactca atgaaaatta aaatcagact aggcgacgca gatgcagata
     4861 gaaccctact actttcacgc tttcaggtag caggctgtaa agctccactg gagctttaca
     4921 ctcatcaaat caagtcaaca acgtctgctt ttaattttcg aagagtatta gataatcgcc
     4981 ttgcgaatga ccacatccac acccttagga gcccaggcag caatcttggc tttttcattg
     5041 acacgaatcc aaccaagtcc tgagaagacc acatccgtct tctcgttgat ggtaaattca
     5101 tgacggacca gttcaggaaa ttctttcaat tccttgctgg ttggtggcac taaaagtgag
     5161 cccgcatgtt tctgataaaa ctcgctagct ccctgcaact tggtgcggtg gagttggagc
     5221 tcattatcaa agaaggccgt aaagccttgg cgttcaccag ccacaaagtc aaatcgtccc
     5281 agaccagcca agaacaaggt ttgttctgga ttgagctggt aggtctttgg cttgatttcc
     5341 ttgcgagggc tgatgtactt gaggttttta gccgtcaaat aatgggccat ctggtgacgg
     5401 tggataattc ctggcgtgtc atagatgtat gaaccatcat ccagagggat ttcaatctta
     5461 tccagcgtcg ttccagggaa acgtgaagtc gtaatgacat ccttgtcccc tgtgatttct
     5521 tgaataatag cgttaatcaa ggttgacttg ccgacattgg tcacgccgac cacatagaca
     5581 tcccgtccct tgcggtgttg ttcaatcttt tcaatcaaat ccttgatagc ctgcttattt
     5641 tgggctgaag tcaagaccac atcaaccggt cgcatgccga tttcatgtgc ccgctcggtc
     5701 aaccactgag taaccttgcc tgtcttgaca gacttgggta aaatgtcctg cttgttgccg
     5761 accaaaagca cgtcatttcc agaaataaag cgaggtaaac ctggaatgac tgacccattg
     5821 aaatcaaaaa tatcaatgac attaaccacc aaggcatcgc tttctcctac gctatggagg
     5881 agtttcaaga aatcatcgtc tgagatattg acatctgaaa tctcattgta atggcgcaaa
     5941 cggaaacaac gttggcagta gagttgccct gtttccaggc ctttttctag ggctgattga
     6001 ggtgtaaaac cagcgacagc cttgtctagc gtctgaatct gggcaccaca gccgatacaa
     6061 aataattctt ccacttacat ctccctcttg tagtctatcg ctccatattt agcgatgatt
     6121 tttttcatag tccgtcgctc acgccaacgg ttaatctgcg tattgataga gtccgtcttt
     6181 atcaagggtt tgaccaagac agacttgaga ccagctcgct tagcagcccg aatatccgtc
     6241 atcagctgat cgccaatcat aattacctca tacggctgga catcaaagcg tttcaaagca
     6301 cggttaatcc caaaggtgaa aggcttgaga gcgaaggctt caaattcaat cccaaatttt
     6361 tcaaccgccc gtttgacacg ttcgtattta ttgttagaca ccaccacaac aggaatacct
     6421 gcgtcctgca aatcatgtaa ccactggcgc atctctggcg taccatcggg attattccaa
     6481 gcaatcaagg tattatccaa gtcaacaaac actactttta tgccatgttt tttcaagctt
     6541 tcgacggtca catcataagc cttttccaag gcaaaatccg gcatgtaatt ttctaaactc
     6601 actgtcgtct ccaaagtttt ttatcagata attataccat ttttctaaga aaaatgcgaa
     6661 tcgatagcag aaaacactcg gatcaaccga gtgttttcta gaggtaatgt aatggtagtt
     6721 gtgaattaaa tctgaccttc ttccaaaatc caatctttcg gaatgaaaat catatgggtt
     6781 agcaatcaat caatatcaag agtaaatttt gaaaaagcaa aaccgatttg ctctactcct
     6841 gactcatcct catccaaccc caaaatggta agagcatgtc cataatctgc atgactatca
     6901 tcgaatagac gccacgcgag agcgcgataa attaacttct tagcatcata gagactgtaa
     6961 cgctgtctgt actctgaaaa tgattgacaa tccttacctt tcggttctac tcctttggtt
     7021 ttagcaatgg caatcaagct atcataatta tgataacgca aagtactatt caaggattgg
     7081 gctaaacgaa cagcatagtc caaagctaaa tcactcacct caacagccac agttctcaat
     7141 tctttacgta ggggattcaa tagagacgca gcagtttcta gtctgtatac cggactttaa
     7201 caaacaaaaa tgaaaacagc cgaacatgtc gactgcttcc gtggagatta tatgaaaaag
     7261 aataaatata ttgtatcttc caatgcttaa acaaacctta acttctaaac atttctgaat
     7321 aaacttcgat tattactctg taatttattt cactttcaaa gctaccttac caaaataatc
     7381 tcgttccatg tacttgattg attggtaatg cttctaataa cgttggcaca ttgtccacag
     7441 tcaatcctcc accaatcaaa atctcaattt taccttgtgc ataggcgaca atttcctgta
     7501 accattccac attgttcaag gcagaccctg tcagactccc acgcgtcaaa atccgtgtca
     7561 ctccatgttc taccagccaa tcaagagcct cgaattggcg ttcgcgagga atttggtcaa
     7621 aagccatgtg aaagacaatc tgacagtcct gcgaaacagc aaacagtctt tccaaggctg
     7681 gttcgtccaa ccaattttcc tcggttaaca aaccataaac cagaccatct gcccccagtt
     7741 caatcgctgc tttacaatct tcaaccatca tttccacctc tgcttcatcg tagcaaaaat
     7801 ctccaccgcg agggcgaatc atggtcatga ctgtggcttc attctcatga gccagttgac
     7861 aaacatgttt gatcactcca taactcggcg tcgttccgcc aaccgctaaa ttatcacaca
     7921 gttcaactct ttgagcaccc agagaaagag ctttcaccac agctacatga ttttcggaac
     7981 aaaattcttt aatcatcttt attcctttca agtctttttt ctttagtat
//
LOCUS       DBNFEG010000101         8028 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.101, whole genome shotgun sequence.
ACCESSION   DBNFEG010000101 DBNFEG010000000
VERSION     DBNFEG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8028
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.101"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(122..1294)
                     /locus_tag="AC1PNG_001430"
     CDS             complement(122..1294)
                     /locus_tag="AC1PNG_001430"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0070085 - glycosylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIP6701931.1"
                     /translation="MIPFALKYIMVIITTMVISAIATPLVRFMSLRVGAVDNPNARRI
                     NKVPMPSAGGLAIFVAFTISVLWFLPGIVTRVNYHGTYLHYIWPLVLASSIIVLTGFI
                     DDVKELKPAPKMFGILAAATIIWIFTEFRFDSFKIPFGGPFLQFPVWLSFLLTVLWIV
                     SITNAVNLIDGLDGLVSGVSIISLTTMGIVSYFFLHDSNIFLTLTIFVLVAAIVGFLP
                     YNYNPAIIYLGDTGALFIGFMIGVLSLQGLKNSTAVAVVTPMIILGVPITDTVVAIIR
                     RKLSGQKIYEADKMHLHHRLLSLGLTHRGTVLVIYTISFLFSLTSLLLNVSSRIGGIL
                     LLVTMGLGIEILCELIGIFGINRTPLLNLLRFIGNSSYRQEKIAKYKKCCEEKKRK"
     gene            complement(1291..2013)
                     /gene="mecA"
                     /locus_tag="AC1PNG_001431"
     CDS             complement(1291..2013)
                     /gene="mecA"
                     /locus_tag="AC1PNG_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HIP6701932.1"
                     /translation="MKVKQISDSTLKITIKLDDLEERGMELSDFLIPQEKTEEFFYTV
                     LDELDLPMTFRESGMLSFRVTPKPDRVDIFVTKSDLDQNLNFDEFTDLSELGDVASMT
                     PDEFFKSLEQTVREKSAPDATAVRHLEEVEQEEEADEEEQERYIYYILEFPTIEDLFT
                     FVGTVDYPVEESELYKMDGHYYLTVLINVENRSKQYPGYILSRMLEFTNDTKLTRPAL
                     QEHGTLMLPLAAIEELRKVPTV"
     gene            complement(2141..2977)
                     /locus_tag="AC1PNG_001432"
     CDS             complement(2141..2977)
                     /locus_tag="AC1PNG_001432"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIP6701933.1"
                     /translation="MLLELLKAIFLGIIEGVTEWLPVSSTGHLILVQEFVKLNQSKNF
                     LEMFNIVIQLGAILAVMTIYFKKLNPFQPGKTKRDIQLTWQLWAKVVIACIPSILIAV
                     PLDNWFEAHFNFMVPIAIALIVYGIAFIWIENRNSGVEPQVTDLAKMSYKTALLIGCF
                     QVLSIVPGTSRSGATILGAIILGTSRSVAADFTFFLGIPTMFGYSGLKAVKYFLDGNS
                     LNMEQVWILLVASMTAYLVSLVVIRFLTDFVKKHDFTVFGYYRIILGAILLVYAFITF
                     LF"
     gene            3104..4669
                     /locus_tag="AC1PNG_001433"
     CDS             3104..4669
                     /locus_tag="AC1PNG_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HIP6701934.1"
                     /translation="MKRKLSIFLLTLIAVFSVTTGVRADEYLRVGMEAAYAPFNWTQE
                     DDSNGAVPIEGTNQFANGYDVQVAKKIAASMDKELLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTEERKKEIAFSDSYYTSIPTLVVRSDSQYANATSLADFAGAKITAQQGVYLYD
                     LIDQIEGANKQTAMGDFSQIRQALESGIIDAYVSERPEGRTAEAANKAFKMVELADSF
                     ETNAEDVTIAVGMRKDDERIAQVNKVLATISEEEQIALMDDMIENQPVEEASEGETPS
                     FFAQVWNIVVNNWQQLLRGTGMTLLISILGTIIGTAIGLLIGVFRTAPKAANKALAIG
                     QKVLGWIINIYIEIFRGTPMIVQSMVIYYGTAQAFGLNLDRTLAAIFIVSINTGAYMS
                     EIVRGGIFAVDKGQFEAATALGFTHNQTMRKIVLPQVIRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTVIAIIYFILTFTVTRILRAVEKHFDTDTYT
                     TGANQMQVEVPHD"
     gene            4662..5405
                     /locus_tag="AC1PNG_001434"
     CDS             4662..5405
                     /locus_tag="AC1PNG_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIP6701935.1"
                     /translation="MTKNVILEIKNLKKSYGQNQVLKDISISVEKGEVISIIGSSGSG
                     KSTFLRSINLLETPTEGQILYHGQDVLDKGYDLTTYREKLGMVFQSFNLFNNLNVLEN
                     AIVAQTTVLKRDRAEAEKIAKENLNKVGMTEQYWQAKPSQLSGGQKQRVAIARALSVD
                     PEVILFDEPTSALDPEMVGEVLKTMQDLAKSGLTMLIVTHEMEFARDVSDRVIFMDKG
                     VIAEEGNPQQIFENPQEERTREFLQRFLG"
     gene            complement(5529..5738)
                     /locus_tag="AC1PNG_001435"
     CDS             complement(5529..5738)
                     /locus_tag="AC1PNG_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701936.1"
                     /translation="MTSYEKVAAVVGWVREKKITGYRISKETNAREMSVIALAQGRAK
                     IDNISFATALGLIDFYDKNHKKFEN"
     gene            complement(5824..6726)
                     /locus_tag="AC1PNG_001436"
     CDS             complement(5824..6726)
                     /locus_tag="AC1PNG_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIP6701937.1"
                     /translation="MVLGPIVFIGSFLFFVLIALILIASGLYVVKQQTVAIIERFGKY
                     QKTSTSGINFKIPFGVDVIAARIQLRMLQSEIVVETKTQDNVFVTMNVATQYRVNENN
                     VTDAYYKLMHPEAQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQKQVAEEMSTYG
                     YVIVKTLITKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRL
                     HGVGIAQQRKAIVDGLADSIRELKESNVSLSEEQIMSILLTNQYLDTLNNFAQGGNQT
                     IFLPGNPEGVEDIRTQILSSLRAK"
     gene            complement(6736..7986)
                     /gene="ilvA"
                     /locus_tag="AC1PNG_001437"
     CDS             complement(6736..7986)
                     /gene="ilvA"
                     /locus_tag="AC1PNG_001437"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019322143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HIP6701938.1"
                     /translation="MITAKNVEQAYSVLKRVVVRTPLDYSRYLSEKYGATIYLKRENE
                     QRVRSFKIRGAYYAISQLTDDEKSRGVVCASAGNHAQGVAYTCQEMQIPATIFMPITT
                     PQQKIGQVKFFGGDYVEIRLVGDTFDESAKAAQDYTQESGKTFIDPFDDENVQAGQGT
                     VAYEILEEAEEQTISFDQILVPIGGGGLVAGVSAYLKEHAPEIKIVGVEASGARSMKA
                     AFDKGRPVKLDQIDKFADGIAVQKVGKSTYEVARKYVDRLIGVDEGWISGTILDLYSK
                     LGIVAEPAGAATIAALEVVKDKIKGQTICCIISGGNNDINRMPEMEERALIYEGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRANKGTGPVLIGIALGDKTDYTQFI
                     SRLEEFDPQYINLHENDSLYKMLV"
BASE COUNT         2480 a         1808 c         1460 g         2280 t
ORIGIN      
        1 tgatgatttt cagagagtgt taatctataa aatagtcttt tctgacaatt ttaattaacc
       61 atatccacta tattatatca aaaaaagagc ctgacggctc ttatcttgtt tagaaagatt
      121 cttattttct ttttttctct tcacagcact ttttatactt tgcgattttt tcttgacggt
      181 aactactatt accaataaac cgaagaagat tgagcaatgg agttcgatta ataccaaaga
      241 tgccaatcaa ttcacagaga atctcaattc ccagtcccat ggtcaccaat aggagaatac
      301 cccctattcg actcgaaaca ttgagcaaaa gagaggtcag ggagaataag aaagagatgg
      361 tgtaaatcac caatactgtg ccacgatgag taagtcccag agagagcaag cgatgatgca
      421 gatgcatctt atctgcttca tagatttttt gcccagacaa ttttcgacga atgatggcta
      481 ctacagtatc cgtaataggc actccaagaa taatcattgg agtaaccact gcaacagctg
      541 tcgaattttt cagtccttga agggacaaga ctccaatcat aaacccgatg aaaagggctc
      601 ctgtatcgcc aagataaatg atagcgggat tatagttata aggtaaaaat ccaacaatcg
      661 ctgcgaccaa aacaaagatg gttaaggtca aaaaaatatt gctatcatgc aaaaagaaat
      721 aagacacaat tcccatcgtt gtcagggaaa taatcgatac tcccgaaact agaccatcca
      781 gaccatcaat caagttgact gcattcgtaa tagaaacaat ccaaaggacc gttaatagaa
      841 aagagagcca gacaggaaat tgaaggaaag gtcctccaaa aggaatctta aagctatcaa
      901 aacgaaattc cgtaaaaatc caaatgatcg tcgctgcagc tagaatacca aacattttag
      961 gagcaggctt tagctccttg acatcatcaa tgaatcccgt aagaacgata atgctggatg
     1021 ctaggaccag gggccagata taatgcaagt aagtgccatg gtaattgaca cgggtaacaa
     1081 ttcctggtaa aaaccatagg acagaaatgg taaatgccac aaagatagcc agacctcccg
     1141 ctgaaggcat gggcacctta tttattcgcc gtgcattggg attatcgact gctccaaccc
     1201 tcagggacat aaaacgaacc aagggggttg caatagctga tatgaccata gttgtaataa
     1261 tgaccattat gtattttaga gcaaaaggaa tcatactgtc ggaaccttcc taagctcctc
     1321 gattgccgca agcggaagca tcaaagttcc gtgttcttgt aaagcaggtc tggtcaactt
     1381 agtatcattg gtaaattcca acattcttga gagaatgtag cctggatact gcttggaacg
     1441 attttctaca ttgataagaa ctgtcaagta gtagtgacca tccattttat ataattccga
     1501 ttcttctact ggataatcaa ccgtaccaac aaaagtaaac agatcctcta tcgttggaaa
     1561 ttccaaaata tagtaaatat agcgctcctg ctcctcttca tctgcttctt cctcttgctc
     1621 tacctcttcc aaatgacgaa ctgctgttgc atccggtgca cttttctcac gaacggtttg
     1681 ctccaaactc ttgaaaaact catctggagt catacttgcg acatcaccaa gttccgacaa
     1741 atctgtaaat tcatcaaaat tcaagttctg atccaaatca gatttggtaa caaagatgtc
     1801 cacccgatct ggcttcggag tcacacgaaa actcaacata ccgctttctc ggaaagtcat
     1861 tggtaaatcc aattcatcca aaacagtata gaagaactcc tctgtctttt cttgcggaat
     1921 gagaaagtcc gaaagttcca ttcctctctc ttccaaatca tctaatttta tagtgatttt
     1981 caaggttgaa tcactaattt gctttacttt cataactacc tcattcattg attcatcaat
     2041 ttcttaaaat tgattgatca ttttcaatac tcatacatat cttccaccat tatactaaaa
     2101 ccaagctcga aagacaaaag aaaagcaggt ctccctgctc ttaaaataaa aatgtaataa
     2161 aggcataaac caataaaatc gcccctaaaa tgatgcggta gtaaccaaaa accgtgaaat
     2221 catgtttttt aacaaagtct gtcaagaaac gaataacaac taaggatact aagtaagccg
     2281 tcatactagc gactagcaag atccatacct gctccatatt caggctattg ccatctaaga
     2341 aatatttaac agccttcaag ccactataac caaacatggt tggaatccca aggaagaagg
     2401 taaaatctgc cgccacagat cggctggtcc ccaaaataat cgctcccaga atggtcgcac
     2461 ctgagcgact agtccccgga acgatgctta agacctgaaa acacccaatc aacaaggcag
     2521 ttttataaga cattttcgct aagtccgtca cctgtggctc tacaccactg ttacggttct
     2581 caatccaaat aaaggcaata ccgtagacaa tcaaagcaat cgcaatcggt accataaaat
     2641 tgaagtgcgc ttcaaaccaa ttatccaacg ggactgcaat taggatggac ggaatacagg
     2701 caatgacaac cttggcccat aattgccaag tcaattgaat atcacgcttg gtttttccag
     2761 gctgaaatgg attcaatttt ttaaagtaaa tagtcataac tgctagaatt gctcctagct
     2821 gaatgacaat attaaacatt tctaggaagt tcttgctttg gtttaatttc acgaactctt
     2881 gaaccaaaat taaatgcccc gttgaagaaa ctggcaacca ttccgtcacc ccttcaatga
     2941 ttcctaaaaa tatagccttc aacaattcca atagcataaa aatctccttt tcatcaattc
     3001 tctacattat atcataaaaa aactccctat tcatagtaca aggatattga aatttgtcag
     3061 aatttttaat aaaatggtgt aatagcattt taaaggagac attatgaaac gtaaactcag
     3121 tatatttctg ctgaccctaa tcgctgtttt ttctgtaaca acaggggttc gggcagacga
     3181 atacctgcgg gtcggtatgg aagctgccta tgcaccattt aactggaccc aagaagatga
     3241 tagcaacggt gccgttccta ttgagggaac caatcaattt gccaacggat atgatgttca
     3301 ggttgcaaaa aaaatcgctg catccatgga caaagaactc ttggttgtta aaaccaagtg
     3361 ggaaggctta gtccctgctc ttacatctgg taaaatcgat atgattatcg ccggtatgag
     3421 cccgacagaa gagcgcaaga aagaaatcgc cttctcagat agctactaca cttcaattcc
     3481 taccctggtt gttcgttctg atagtcagta cgcaaacgca accagcttgg cagattttgc
     3541 tggcgcaaaa atcactgccc agcaaggggt ttacctctat gatttgattg accaaattga
     3601 aggggctaac aaacagactg ccatgggaga tttctcacaa atccgtcaag ctttagaatc
     3661 tggcattata gacgcctatg tttctgaacg tccagaaggt cgtacagcag aagctgcaaa
     3721 taaagctttc aaaatggttg aattggcaga cagttttgaa accaatgccg aagacgtgac
     3781 cattgctgtc ggtatgcgca aagatgacga gcgtatcgct caggtcaaca aagtcttagc
     3841 aactatttca gaagaagagc aaatcgcact tatggatgat atgattgaaa accagcccgt
     3901 tgaagaagcc agcgagggcg aaacacctag tttcttcgcc caagtttgga atattgttgt
     3961 taacaactgg caacaacttc tgcgcggaac tgggatgaca ttactcattt ccatcctcgg
     4021 tacaattatc ggtactgcta tcggtctcct catcggtgtc ttccgtacag ctccaaaagc
     4081 agcaaacaag gcgcttgcta ttggtcaaaa agtgctcggt tggattatca atatctacat
     4141 cgaaattttc cgtggtacgc cgatgattgt acaatccatg gttatttact atggtaccgc
     4201 ccaagcattc gggctcaatc ttgaccgcac acttgccgct atcttcattg tatccatcaa
     4261 cacaggtgcc tacatgagtg agattgtccg cggtggtatc tttgccgtcg ataaaggcca
     4321 atttgaagct gctactgctc tcggatttac tcataatcag accatgcgta agattgtcct
     4381 tccacaggtt attcgtaaca tcttaccagc gactggtaat gaatttgtca tcaatatcaa
     4441 agatacttct gtattgaacg ttatctcagt tgttgaactc tatttctcag gaaatactgt
     4501 cgcaacccaa acctatcaat atttccaaac ctttacggtt attgctatta tctacttcat
     4561 cctaaccttt actgtgacac gtatcttacg cgcagttgaa aagcattttg atacagatac
     4621 atacacaaca ggtgcaaacc aaatgcaagt cgaggtgcca catgactaaa aatgtaattc
     4681 ttgaaatcaa aaatctaaag aaatcatacg gacaaaatca agtcctcaaa gacatctcta
     4741 ttagcgttga aaagggcgag gttatctcaa tcatcggttc atctggttcg ggaaaatcaa
     4801 ccttcctccg atctatcaac cttttggaaa caccaacaga aggacagatt ctataccacg
     4861 gacaagatgt tttggataaa ggatatgact tgacgactta ccgtgaaaaa ctaggtatgg
     4921 tattccaatc cttcaacctt ttcaacaatc taaatgtatt ggaaaatgcc attgtagcac
     4981 aaactacggt actgaaacga gatcgagctg aggcagagaa aatcgccaag gaaaacctca
     5041 acaaggttgg catgaccgaa cagtactggc aagccaaacc aagtcaactc tctggtggac
     5101 agaaacaacg cgtggccatt gcacgtgccc tatctgttga cccagaagtt atcctctttg
     5161 atgagccgac ttctgctctc gatccagaga tggtcgggga agttctcaaa accatgcaag
     5221 atttggcaaa atcaggattg acgatgctta tcgtaactca cgaaatggaa tttgcccgtg
     5281 acgtatctga ccgcgttatc tttatggata agggtgtcat cgccgaagaa ggcaaccctc
     5341 aacaaatctt tgaaaatcca caagaagaac gcacacgtga attccttcag cgttttcttg
     5401 gttaaactca atctgttcca tagaagacca ttacttacca gtttcaaaac tggtaagttt
     5461 ttttgactaa aaaaaagagg aatcgcaaaa ttcctctttt tggacatctc taaatgaaac
     5521 catttagatt agttttcaaa tttcttgtgg tttttgtcgt aaaaatcaat caaacccaag
     5581 gcagttgcaa atgaaatatt gtcaatctta gcgcgacctt gtgcaagagc gataactgac
     5641 atttcacgag catttgtttc cttactgata cggtaacctg tgattttctt ttcacgaacc
     5701 cagccgacaa cagcggctac tttttcgtaa ctagtcatta gaaacccctt tcttttttat
     5761 tcgtgcaatt tagtataata tgttttaacc tattttgtca agtaaaatgt tcgtttttta
     5821 ccatcatttt gccctaagag atgatagtat ttgcgtcctt atgtcttcga ccccctccgg
     5881 attccccgga aggaagatag tttgattacc tccttgtgca aaattattga gagtatccaa
     5941 gtattggttg gtcagcaaga tagacataat ctgctcttca gataggctaa catttgattc
     6001 tttcagttca cgaatcgaat ctgccagtcc gtctacaatc gccttacgtt gttgtgcaat
     6061 acctacacca tgcaagcgat ccttctcagc ctctgcttct gctgctgtga caattttaat
     6121 cttgtcagct tctgccaatt cttgggccgc aacccgtttc cgctgagctg cgttaatttc
     6181 attcatagat tgcttaacct cggcgtctgg ctctaccttg gtaattaagg ttttgacaat
     6241 aacataaccg taggttgaca tctcttctgc tacttgtttt tgtacctcaa gtgcaatctc
     6301 atcctttttc tcgaagagct catccagggt caatttaggc acagatgaac gcagagcatc
     6361 ttcaatatag gacttaatct gtgcttctgg gtgcatgagc ttgtaatagg catccgtcac
     6421 attattttca tttactcgat attgagttgc cacattcatt gtgacaaaaa cgttatcctg
     6481 cgtcttggtt tctaccacaa tttcactttg caacatccgc aattgaatgc gagctgcaat
     6541 gacatcaact ccaaatggta ttttaaagtt aatacctgaa gtagatgttt tttggtattt
     6601 cccaaagcgc tcaataatag cgaccgtctg ctgcttgaca acgtatagcc ctgaagcaat
     6661 caaaatcaaa gcaatcagta caaagaacaa gaaactacca ataaaaacaa ttggaccaag
     6721 caccataatg acctcctaaa ctagcatttt ataaagcgaa tcattttcat gaagattgat
     6781 gtactgggga tcaaactctt ccaagcgaga aataaactgc gtataatctg ttttatctcc
     6841 tagggcaata ccgattagaa ccggccctgt tcccttattg gcacgtttga tatattcaaa
     6901 acgagtaata tcatcatttg gccctagaat atcatttaca aattcacgaa gagcccctgg
     6961 acgttgtgga aaattaacaa caaagtagtg tttaattccc tcgtagataa gcgcacgctc
     7021 ctccatttcc ggcatcctgt tgatgtcgtt attaccacca gaaatgatac aacaaatggt
     7081 ttgccccttg attttgtcct tgactacctc aagagctgca atcgttgctg ctcctgctgg
     7141 ctcagcaaca atacccagct tagaatagag gtctaaaata gtcccggaaa tccatccttc
     7201 atccacacca atcaggcgat ctacgtattt ccgagccact tcgtaggtcg acttaccgac
     7261 tttctgtacc gcaataccgt cagcaaattt atcaatttgg tctaatttaa ccggacgacc
     7321 tttatcaaaa gccgctttca ttgaccgtgc cccacttgct tcaacaccaa caatcttaat
     7381 ttcaggtgca tgttctttca gataggccga aacgcctgca accagaccac ctcctcctat
     7441 gggaactaaa atttggtcaa aactaatggt ttgctcttcc gcttcttcca agatttcata
     7501 ggctactgtt ccctgaccag cctgcacatt ttcatcatcg aatgggtcta taaatgtttt
     7561 cccactttcc tgagtataat cttgtgctgc cttggctgac tcatcgaagg tatcacctac
     7621 gagccgaatc tctacgtagt ctcctccgaa aaatttaacc tgccctattt tttgttgcgg
     7681 agttgtaatg ggcataaaaa tcgtagcagg aatttgcatt tcctgacagg tgtaggccac
     7741 accttgagca tggtttccgg ccgaagcaca gaccactccg cgtgacttct catcatctgt
     7801 caattgtgaa atagcataat aggctcctcg aattttaaaa gagcgaacac gctgttcatt
     7861 ttctcttttt aaataaatag ttgcaccata tttttccgat aagtatcgac tataatccaa
     7921 gggggtacgt actaccacgc gcttaagaac ggagtatgct tgttctacat tttttgctgt
     7981 aatcatagtt ccattaattt ctttctatat ttattgtatc aatattcc
//
LOCUS       DBNFEG010000102         7926 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.102, whole genome shotgun sequence.
ACCESSION   DBNFEG010000102 DBNFEG010000000
VERSION     DBNFEG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.102"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..1333)
                     /locus_tag="AC1PNG_001438"
     CDS             complement(<1..1333)
                     /locus_tag="AC1PNG_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HIP6701939.1"
                     /translation="MGEKNSHHLPLDEEKVLDFEVAKDLTIEEAVKKHKEIEAGVTED
                     DGLLDRYIKQHRAEIESQKFETKINHLPLVEVADEEKNQGHESAEEVEANESSLTEVS
                     EEIAPIVEELSVAPMETLEETVIASTVAMEGLSSVADDSSLELEEDEAEDLDHSEGAD
                     RDQKKKFYFWSAVGLSMIGVMVTALIWMNSVNKSNTATSSSSTSTSQTSSTASSSTDA
                     NVTALEQLYNSFFTDSSLTKLKNSEFGKLAELKVLLEKLDKNSDSYTKAKEQYDHLEK
                     AIAAIQAINGQFDKEVVVNGEIDTTATVKSGESLSATTTGISAVDSLLASVVNFGRSQ
                     QEVASATVASEAAVTSSQGADETVSTGVPATTEVASTTVSGSTTDFGIAVPAGVVLQR
                     DRSRVPYNQAMIDDVNNEAWNFNPGILENIVTISQQRGYITGNQYILEKVNI"
     gene            complement(1343..2509)
                     /locus_tag="AC1PNG_001439"
     CDS             complement(1343..2509)
                     /locus_tag="AC1PNG_001439"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIP6701940.1"
                     /translation="MKKQFELIATAAAGLEAVVGREIRNLGYECQVENGRVRFQGDVK
                     SIIETNIWLRSADRIKIIVGQFPAKTFEELFQGVFNLDWENYLPLGCKFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHFSRPEGVPLQEMGAEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRVEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAAMLAKN
                     IAPGLKRSFAFEEWPWVEDQLVAALRKEAQASIKTDLVLDIAGSDIDARMIEIAKKNA
                     FAAGVEQDIVFKQMRVQDLRSDKINGVIISNPPYGERLLDDEAIVTLYREMGQTFEPL
                     KTWSKFILTSDELFETRFGQQADKKRKLYNGTLKVDLYQFFGQRVKHQVQEVQG"
     gene            complement(2563..2927)
                     /gene="rnpB"
                     /locus_tag="AC1PNG_001440"
     ncRNA           complement(2563..2927)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC1PNG_001440"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(3066..3401)
                     /gene="gpsB"
                     /locus_tag="AC1PNG_001441"
     CDS             complement(3066..3401)
                     /gene="gpsB"
                     /locus_tag="AC1PNG_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HIP6701941.1"
                     /translation="MASIKFTTKDIFEQDFKIGFRGYDQDEVNDFLDDIMKDYDAYEA
                     IIKELKGEIARLKAQAANSPKTTLPTEESNDVLRTERPSSATNFDILRRLNRLEKEVF
                     GKQIVQDQE"
     gene            complement(3469..4008)
                     /locus_tag="AC1PNG_001442"
     CDS             complement(3469..4008)
                     /locus_tag="AC1PNG_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HIP6701942.1"
                     /translation="MDTKSLLVTGYRHTDLGIFSEKDPRLHIIKSAIRRNFIRFLEEG
                     VSWFILTGQLGFEYWSLEVLEDLRAEGYQLSIATIFPFENHGEQWNEANQAKLARFKQ
                     VDFVKYAYPRYENPGQFRDYNQFLLDNTTGCYLFYDSENETNLKYLYHMVLKKEGYNR
                     KTLTFEELNEEAENFSNSE"
     gene            4074..4691
                     /gene="recU"
                     /locus_tag="AC1PNG_001443"
     CDS             4074..4691
                     /gene="recU"
                     /locus_tag="AC1PNG_001443"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HIP6701943.1"
                     /translation="MVNYPHKVSKKINRTSPISSQRVNFANRGMSFEAAINDSNQYYL
                     AHDIAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYSGVFKRHYIDFEAKET
                     RQKASMPMKNFHAHQIEHMKQVVKQGGICFVLLHFSTLKETYLLPATHLIEFYQVDMG
                     SKSMPLTFIRQYGFEIQMGRFPSIPYLEIVEKNLLGGESFENYNN"
     gene            4672..6846
                     /gene="pbp1a"
                     /locus_tag="AC1PNG_001444"
     CDS             4672..6846
                     /gene="pbp1a"
                     /locus_tag="AC1PNG_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1A"
                     /protein_id="HIP6701944.1"
                     /translation="MKTTTIKKALLITANILLAGAIIACLAGAALVVYYIQSAPELTE
                     ESLTATVSSKIYDKNGNLIADLGAEKRSSAKTEEIPTDLVNAIVAIEDQRFFNHRGVD
                     VIRIAGSLINNLSGGRLQGGSTLDQQFIKLTYFSTSVEDQNLKRKIQEAWLATQLERR
                     NTKQEILTYYINKVYMSNGNYGMKTAALAFYGKELKDLTLPQLALLAGMPQAPNQYDP
                     YTNPEDAKARRDLVLAEMLEEKYIDNPQYEQAVLTPVTDGLQPLSNAAAYPAYMDNYL
                     KQVVEEVEAKTGYNLLTTGMDVYTNVDPAAQQQLWNIYNTDMYVSYPDDLLQVASTVV
                     DVSNGKVVAQLGGRKQETNVSFGTNQSVETNRDFGSTMKPITDYAPAFENGIYTSTAD
                     LILDGPYNYPGTPTPVNNWDKQYYGNISVKTAIQYSRNVTAVKALEATGLENALKFLN
                     SVGINYPDIHYSNAISSNTSDTSSKYGASSEKMAAAYAAFANGGTYYAPQYVNKIVFS
                     DGTVTEYAPKGTKVMSAETAYMMTDMMKAVMSYGYGLNASVSGVPMAGKTGTSNYTDS
                     ETDTILASIPEANYSYMVVPDENFVGYSSQYAMAVWTGYTNRMTPILDNSMRIATDVY
                     HNMMLFMHSDYTATDWEMPSGLVRYGSNYYLRGSRSLSNAYNTYTSSSTSSSSSTETS
                     ETTEATTTSSETSSGTATETATSSTETATTGNNPATGQTDGQ"
     gene            6905..7618
                     /gene="rsmG"
                     /locus_tag="AC1PNG_001445"
     CDS             6905..7618
                     /gene="rsmG"
                     /locus_tag="AC1PNG_001445"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIP6701945.1"
                     /translation="MKPEVFYKTLADQGIQLTDQQKHQFHRYFQLLVEWNEKINLTAI
                     TEESEVYLKHFYDSIAPILQGHIQNEPLRLLDIGAGAGFPSLPMKIIFPQLDVTIIDS
                     LNKRINFLHLLAEELELEGVHFYHGRAEDFAQDKNFRAQFDLVTARAVARMQILSELT
                     IPYLKLNGKLIALKASSAEDELTQAKNALNLLFAKVIENHDYTLPNGDPRTLTIVEKK
                     KETPNKFPRKAGLPNKRPL"
     gene            complement(7808..>7926)
                     /locus_tag="AC1PNG_001446"
     CDS             complement(7808..>7926)
                     /locus_tag="AC1PNG_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIP6701946.1"
                     /translation="FSEIQSNSFKSLEEGQKVTFEVEQGQRGLQATNITKIG"
BASE COUNT         2343 a         1796 c         1393 g         2394 t
ORIGIN      
        1 taatattaac tttttccaag atatattgat tgcctgttat atacccacgt tgttgcgaga
       61 tagtcacaat attttcaaga atacctggat tgaaattcca tgcttcattg tttacatcat
      121 caatcattgc ttgattataa ggaacacgac tgcggtcacg ttgcaagacg acaccagctg
      181 gcacggcaat accaaagtcg gttgttgagc ctgaaaccgt tgttgaagcg acttctgttg
      241 tcgctggtac accagtagat acagtttcat cagcaccttg gctgcttgtg actgcagcct
      301 cacttgctac cgtagccgaa gcgacttcct gttgtgaacg accaaaattc acgaccgatg
      361 ccaatagact gtcaaccgca ctaatccctg tcgttgtcgc agaaagagat tctcctgatt
      421 taaccgtcgc cgttgtatca atttcaccat taacaacgac ttctttatca aactgaccat
      481 taatggcctg aatggctgca atggcttttt caagatgatc atactgttct ttagctttgg
      541 tataactgtc cgaattctta tccaattttt ccaacagcac tttcagttct gctaatttac
      601 caaactctga attttttagc ttggtcaaac tactatccgt aaagaaacta ttatagagct
      661 gttctaaagc ggtcacattc gcatctgtcg aacttgatgc tgtcgatgac gtctggcttg
      721 tacttgttga acttgaagaa gtcgcagtat tcgatttgtt aaccgaattc atccaaatca
      781 gcgccgtcac cattacacca atcattgaaa gccctactgc tgaccagaag taaaatttct
      841 ttttctggtc cctatctgcg ccctcactat ggtccaagtc ttcggcttca tcttcttcca
      901 attctaaact agaatcatct gccactgaag ataacccctc cattgctaca gtacttgcaa
      961 tcactgtttc ttccaaggtt tccatcggag ctaccgaaag ttcttctaca ataggagcaa
     1021 tctcctcact aacttccgta agagaagact catttgcttc tacctcttcc gcgctttcat
     1081 gtccttgatt tttttcctca tcagccacct caactaaagg aagatgattg attttggttt
     1141 caaatttttg agattcaatt tctgcacggt gttgcttgat gtaacgatcc aagagaccat
     1201 catcttctgt cacacccgct tcaatttcct tatgtttttt gactgcttct tcgattgtta
     1261 aatctttcgc cacttcaaaa tctaaaactt tctcctcatc caaaggaaga tgatgactat
     1321 ttttttctcc cacgatgtcc tcctatcctt gcacttcttg cacttgatgt ttcactcgtt
     1381 gaccaaaaaa ttgataaaga tccactttta gggtaccatt atacagtttg cgtttcttat
     1441 ccgcttgctg tccaaaacgg gtttcaaaca actcatcact agttagaata aacttagacc
     1501 atgttttgag tggttcaaaa gtctgcccca tttctcgata aagggtaaca atggcttcat
     1561 catccaacaa acgttctccg taaggtggat tagaaatgat gacaccatta attttatctg
     1621 aacgcaagtc ttgcacacgc atttgcttga aaacaatatc ctgctcaact cctgccgcaa
     1681 atgcattctt cttcgcaatt tcaatcatac gagcatcaat atctgatcca gcaatatcta
     1741 ataccaaatc tgttttgata gaagcttgcg cttctttgcg taatgcagcc accaattgat
     1801 cctcaaccca cggccattcc tcaaatgcaa aacttctctt caaacctgga gctatatttt
     1861 ttgccaacat agctgcctca atacagaaag ttcccgatcc gcaagtaggg tcaataagcg
     1921 gtttatctgg ataccaattc gataattgca aaatagcagc cgccatattt tctttgattg
     1981 gtgcgccacc tttttccacc cgataaccac gtttgaacaa actagaacct gtcgtatcaa
     2041 tcatgactgt cgccacatct ttcaaaatcg acacttcaat tttaaactct gcccccatct
     2101 cttgcaaggg aaccccttcg ggacgagaga agtgtttctg cagtttcttt acaactgctt
     2161 tcttagaaat agcctgcaca cttggttcat tatgcaattt cgatttgaca catttggcct
     2221 tggaaatggg gaacttgcag cctagaggca agtaattttc ccaatccaag ttaaaaactc
     2281 cttggaacaa ctcttcaaaa gtctttgctg gaaattgtcc aacaatgatt ttaattctat
     2341 ctgctgaacg cagccagata ttggtttcga taatggattt tacatctcct tggaatctaa
     2401 cacggccatt ttccacttgg cattcatagc cgagattacg aatttctcga cccacaaccg
     2461 cttccagacc agctgctgcc gttgcaatca actcaaattg ttttttcatt tttttcctct
     2521 acaataaaag ctagcagagc tagctaccta ttttatgcaa ttttctgtaa gccatgtttt
     2581 gttccagtgg tactaggttc taccaccttc gataatcatc tgtctactgt ctaaacagtc
     2641 aggatttctg ttcatttcca tccatcccat gccccgacca aagtttgggt tgctagcttg
     2701 aggggtttac cgcgttccat tttttacgtt tccataaaaa cttcgtcact gtggcacttt
     2761 cagatctact ctgacatatc aaaagactta gccattttaa ccgccgtaac gcccaagggc
     2821 gtccctaggc ttattgattc gcctagcaca aacactactg gcatctcagc cagtgctagc
     2881 atggactttc ctcatggtag aaaactacca cgcgattatc caaaaattgc accttaatta
     2941 tcttagaagt tgtatctaca aagcaagcca ctttaaatca aggaatatct ctgacacaaa
     3001 tacagaatgt atttgtgcac aagaaaataa ctgcaaataa agtgtcagtt taggtgtaca
     3061 attttctatt cttgatcctg aacgatttgt tttccaaata cttctttctc cagacgattc
     3121 agacggcgga gaatatcaaa gtttgtcgct gaagatggac gttctgtacg caatacatcg
     3181 ttcgattctt cagtaggaag agttgttttg ggggaatttg ctgcctgcgc tttcaatcga
     3241 gcaatttcgc cttttaattc cttgataatc gcttcataag catcatagtc tttcataata
     3301 tcatcaagaa aatcgttcac ttcatcttga tcatagccac gaaaaccaat tttaaaatct
     3361 tgctcgaaaa tatcttttgt cgtaaattta atacttgcca tctctctccc taatctaaaa
     3421 cgtcactact cctattatat taaaaaaaca gggaaaaatc aagtcaaatc actcagaatt
     3481 tgaaaaattt tcagcctcct cattcaattc ctcaaaggta agcgtttttc tattatatcc
     3541 ttctttcttt aagaccatgt gatataaata tttcaaattg gtttcatttt cagaatcgta
     3601 aaataaataa caacctgtcg tattatccag tagaaactgg ttatagtctc taaactgccc
     3661 tgggttttca tagcgtggat aggcatactt tacaaaatca acctgcttaa accgtgctaa
     3721 ctttgcttgg ttagcctcat tccactgttc gccatggttt tcaaatggaa atattgttgc
     3781 aatactcagt tgatatccct cggctcgtaa atcttctaag acttctaagg accagtattc
     3841 aaaacccaat tgacctgtca gtataaacca actaactccc tcttctaaaa aacgaataaa
     3901 attcctacga attgcagatt taataatgtg cagtcgagga tctttttctg aaaaaattcc
     3961 caggtctgta tgtctataac ctgttaccaa aaggctctta gtatccattt cttgtactct
     4021 tttcgctaat tatggtataa tatgctgata ttattttatc aagaaggagt tctatggtca
     4081 attatcctca taaggtgtca aaaaaaatca atagaaccag tcctatttca tcccagcgag
     4141 taaactttgc taatcgcgga atgtcttttg aggccgctat caacgatagc aatcaatatt
     4201 accttgcgca cgatattgct gtcattcaca agaagccaac tcccgtgcaa attgtaaaag
     4261 ttgattaccc caaacggagc cgtgctaaga ttgtcgaagc atactttcga caggcatcta
     4321 ccacagacta ctctggtgtt tttaaaagac attatattga ttttgaagcg aaagagacac
     4381 gccaaaaagc atccatgccc atgaaaaatt ttcatgcgca tcagattgaa cacatgaaac
     4441 aggttgttaa acaaggtggt atttgttttg tccttcttca cttttcaaca ttaaaagaaa
     4501 cctatctctt gccagccact catttgatcg aattttatca agtagacatg ggtagtaagt
     4561 cgatgcctct aacctttatt cgacaatacg gctttgagat tcaaatgggg cgtttcccca
     4621 gcatcccata tctggaaatc gttgaaaaaa atctattagg tggtgaatct tttgaaaact
     4681 acaacaatta aaaaagctct tctaatcact gcaaatattt tacttgcggg ggctatcatt
     4741 gcctgcctgg ccggagctgc attggttgtt tactacatcc aaagcgctcc agaattgaca
     4801 gaagaatctt tgacagcaac tgtgtcaagt aaaatttacg ataagaacgg aaatcttatt
     4861 gcagatttag gagcagaaaa acgttcgagt gccaagactg aagaaattcc aacagacttg
     4921 gtaaatgcga ttgtcgcaat cgaagaccaa cgcttcttca atcatagagg tgttgacgtg
     4981 attcgtattg caggttccct gattaataac ctgagtggtg gacgtttgca aggtggctcg
     5041 acacttgacc agcagtttat caagctgact tatttctcga catccgtaga agatcaaaat
     5101 ctaaaacgga aaattcaaga ggcttggcta gctactcaat tggaacgtcg caataccaaa
     5161 caagagattt tgacctacta catcaacaag gtgtatatgt caaatggtaa ttacggtatg
     5221 aagactgctg ctcttgcttt ttatggaaaa gagttaaaag atttgactct tcctcaatta
     5281 gctcttttag caggaatgcc ccaagcgcca aaccaatatg atccttatac caatccagaa
     5341 gatgcaaaag cacgtcgcga tttggtattg gctgagatgc tggaagaaaa gtacattgat
     5401 aatccccagt atgaacaagc tgttctaaca cctgtaacag atggtttgca accactatct
     5461 aatgcagctg cttatcctgc ttatatggac aactatctga aacaagtcgt tgaagaagtt
     5521 gaagcaaaaa caggttacaa cttgctgaca acagggatgg atgtctatac gaacgtcgat
     5581 cctgccgccc agcagcaact ttggaatatc tacaatacag atatgtatgt cagctatcca
     5641 gatgatttat tgcaggtagc ttcaacggtt gtggatgttt ctaatggtaa agtcgttgcg
     5701 caattaggtg gtcgtaaaca ggaaacaaac gtctcatttg gtaccaacca atccgttgaa
     5761 accaaccgcg acttcggttc tacaatgaag ccaatcacag actatgctcc tgcttttgaa
     5821 aacggcattt atacatctac agcagattta atcttagacg gtccttataa ctatcctggt
     5881 acaccaactc ctgtcaataa ctgggataaa caatactacg gtaatatctc tgttaagact
     5941 gccattcagt attctcgaaa tgttaccgcc gttaaagcat tggaagctac gggcttagaa
     6001 aatgccttga aatttttgaa tagcgttggt atcaattatc cagatattca ttactctaat
     6061 gctatttcaa gtaatacatc cgatactagc agtaagtatg gagcaagtag tgaaaaaatg
     6121 gctgctgcct acgctgcttt tgccaatggt ggtacttact acgctccaca gtatgtcaat
     6181 aaaattgtct ttagtgatgg aacagtaaca gaatatgctc ctaaagggac caaagtcatg
     6241 tctgccgaaa cagcctacat gatgaccgat atgatgaagg ctgttatgtc ttacggttat
     6301 ggattaaacg cttctgttag tggtgttcct atggccggta aaacaggtac atcaaactat
     6361 accgatagcg aaacggatac tattctagct tcaattccag aagcaaatta tagttacatg
     6421 gtcgttcctg atgaaaactt cgttggatat tctagtcagt acgcaatggc tgtttggacc
     6481 ggatatacca atcgtatgac tcctatcctt gataacagca tgcgaattgc aacggatgtc
     6541 taccacaata tgatgctctt tatgcattcg gactatacag ctaccgattg ggagatgcca
     6601 agcggactcg tacgttacgg ttccaactac tatctcagag gtagtcgctc actctccaat
     6661 gcttataata cttacactag ctctagtaca agttcttcta gttcgactga aactagtgaa
     6721 acaaccgaag cgacgacaac atcaagcgaa acatcatctg gaactgcaac cgagactgca
     6781 acttcttcaa ctgaaactgc aacaacagga aacaatcccg caacgggaca gacagatggt
     6841 caataagaaa attcccagac tcttctgggt ttttcttttg cttaaattat gataagataa
     6901 gattatgaaa cctgaagtat tttataaaac cttggcagac caaggtattc aattaactga
     6961 ccaacaaaaa caccaatttc atcgctattt ccaactatta gtggaatgga atgaaaaaat
     7021 caatctgaca gctattaccg aggaaagcga agtctatctg aagcatttct acgattctat
     7081 cgctccaatc ctgcaaggcc atattcagaa cgaacctctt cggcttttag atattggagc
     7141 tggagctgga ttccctagcc tacctatgaa gattatcttt ccgcaactag atgttaccat
     7201 cattgattct cttaataaac gaattaactt tttacatttg cttgccgaag aactagagct
     7261 cgaaggtgtt catttctatc atggtcgtgc agaagacttt gcacaagata agaacttccg
     7321 tgcacaattt gaccttgtca cagcacgcgc agttgcccgc atgcagattc tttctgaatt
     7381 gactattcct tatttgaagc tcaacggaaa attaatcgca ctcaaggcgt ccagcgcaga
     7441 agatgaattg acccaagcaa aaaatgcact caatctcctc ttcgcaaaag tcatcgaaaa
     7501 ccacgactat actcttccaa atggcgaccc tcgaacattg accatcgtcg aaaagaaaaa
     7561 ggaaactcca aataagtttc cacgcaaagc tggtctgcca aataaaagac ccctttaaac
     7621 ataagaaaag aggctgggca aaaagctcaa cctcgcttct cagagttcgt gtcaacatct
     7681 cagcgcagtg gttgattggc agatttgttc gtgttttaca ctccaaatct gacctaatca
     7741 actgtgcgga ggcgggaaga cgaactcttt ttttgaccag tcgagttctt tcccgctccc
     7801 tcttttatta tccaattttt gtaatattgg ttgcttgaag accacgttgg ccttgctcca
     7861 cttcgaatgt cactttttga ccctcttcca acgatttgaa actattagac tgaatttcag
     7921 aaaagt
//
LOCUS       DBNFEG010000103         7878 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.103, whole genome shotgun sequence.
ACCESSION   DBNFEG010000103 DBNFEG010000000
VERSION     DBNFEG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.103"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..191
                     /gene="dltC"
                     /locus_tag="AC1PNG_001447"
     CDS             <1..191
                     /gene="dltC"
                     /locus_tag="AC1PNG_001447"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HIP6701947.1"
                     /translation="DVSEMMDEDLFDAGVLDSMGTVELIVELESRFNITVPVSEFGRE
                     DWNTANKIISGVVELMHA"
     gene            184..1449
                     /gene="dltD"
                     /locus_tag="AC1PNG_001448"
     CDS             184..1449
                     /gene="dltD"
                     /locus_tag="AC1PNG_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HIP6701948.1"
                     /translation="MRKQLWQIFGPVICATFLVILTLLFFPSSSRSNFKEEKQDAVAL
                     SYISFKSKYKKNRALGDKNHRFVPFFGSSEWSRFDSMHPSVLADAYQREYTPYLLGQR
                     GAALLTQYFGMQQIGAHLKNSQAIYVVSPQWFSPKGASSKAFEQYFSSDQATHFLQTS
                     AGDDYDRYAAKRFLELKSDCSLTGMFQKVASGKELSKWDREQLTWQAYLLEKQDALFS
                     RFADSGSNLGLVETEAMKLPETFSYDRLSKVANEDGSRSTQSNDFQIDDRFYRNRLQS
                     KIKKLKQSQTKISYLQSPEYNDFQLVLNEFAKHQTDVLFVIPPVNERWMNYTGLNKDM
                     YRATVAKIKYQLTSQGFNHIADLSEQGGIDYFMQDTIHIGWQGWLELDKYIHPFLTEQ
                     QAPVHYQLKKEFLTSEWATYKGDLNLFLK"
     gene            complement(1538..2632)
                     /locus_tag="AC1PNG_001449"
                     /pseudo
     CDS             complement(1538..2632)
                     /locus_tag="AC1PNG_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
     gene            complement(2706..3662)
                     /locus_tag="AC1PNG_001450"
     CDS             complement(2706..3662)
                     /locus_tag="AC1PNG_001450"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIP6701949.1"
                     /translation="MGLLQDGKWVDQWYDTKSTGGKFIRSVTQFRNWITPDGQAGPTG
                     EGGFKAESGRYHLYISLACPWASRTLIMRKLKGLEDHISLSVVHPLMLENGWTFEDGP
                     GVIKDTLFNSDYLYQVYLKADSHYSGRVTVPVLWDKETNTIVSNESAEIMRMFNSAFN
                     DITGNYDDYYPANLQAEIDAMNDFIYPNINNGVYKAGFSTSQAVYEKEVKNLFAALDK
                     LEEHLADKDYLVGNQLTEADIRLFTTLVRFDPVYFGHFKCNIKALVDYPNLWDYTKRI
                     YNHSGIAETVDFDHIKQHYYGSHKTINPTGIVPVGPDLDWTL"
     gene            3801..4517
                     /locus_tag="AC1PNG_001451"
     CDS             3801..4517
                     /locus_tag="AC1PNG_001451"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002282339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIP6701950.1"
                     /translation="MRLDKCLEKAKVGSRKQVKKLFKAQQIKINGQAAQSLSQIVDPE
                     LQTIQVSGKKVALEGSAYYLLHKPAGVVSAVTDQEHQTVIDLISPQDSREGLYPVGRL
                     DRDTEGLVLITNNGPLGYRMLHPSHHVDKFYYVEVNGCLAEDASKFFASGVTFLDGTR
                     CQPAVLTVLEASLDHSRATIKLAEGKFHQVKKMFLAYGVKVTYLKRISFGGFELGDLE
                     RGAYRQLTPNEMEHLFTYFD"
     gene            4531..5010
                     /locus_tag="AC1PNG_001452"
     CDS             4531..5010
                     /locus_tag="AC1PNG_001452"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIP6701951.1"
                     /translation="MSIYDFTVQKQDGTDQSIAEYQGQVLLIVNTAPGCGLAPQYKEL
                     QELYDSYRGQGFVVLDFPCNQFLNQAPGSAEDINQTCSLNYGTTFPRFAKIAVNGSEA
                     SPLYRYLKKEKSTLLGGRIEWNFTKFLVDRQGRVVKRYLPTTSPLKLKEDIEFYLEK"
     gene            complement(5029..6030)
                     /locus_tag="AC1PNG_001453"
     CDS             complement(5029..6030)
                     /locus_tag="AC1PNG_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIP6701952.1"
                     /translation="MIRHHNYLKIFTFLLILLFVIFLLSLSIGYSNSSFSDLIDIMSG
                     RASSSTLLIIGRIRLPRIIASIIGGASLALAGLLLQTLTRNPLADSGILGINAGAGLV
                     VALFVGLSSKISPVLLSLMPLFAMMGGGLTIFLVYWIARKKLYGIHPARLIITGVGIS
                     SMLSGIMVSIISQLDDFKMEYIVQWLSGRVNGGDWKTIAIYTPLLLLVWMATFSRSRS
                     LNIMNLNDQTAMALGLHLQRERRITLILATALASLSVILVGNITFVGLIAGHIVRKWL
                     GNDHRVIIPATMIIGSFILLLADTIGRVLLVGTGIPTGIIVSLIGAPYFLWLLKQEG"
     gene            complement(6027..7052)
                     /locus_tag="AC1PNG_001454"
     CDS             complement(6027..7052)
                     /locus_tag="AC1PNG_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIP6701953.1"
                     /translation="MNKLVSSRYPKTKPKNIWLVFFIICLSFIAGVYLSLRFGAISYS
                     HQQLIETLKHPLTDSSAQDVIIDLRLPRMVAAILVGAAMAQAGAMMQGITRNPIADPG
                     LLGINAGAGLALIVAYALFGGLHYSQILLICLLGSCLAAGLVFGLAYQVQKGYNQLRL
                     ILSGAMVASLFSAIGQAITIYFDLSTAVIGWQAGGLVQVNWKMLAIIAPLIIIGLILA
                     QLFSHQLTILSLNETVAKNLGQRTLLMTLVLLGIVLLLSASAVALVGSLSFIGLIIPH
                     FIRMFTGKNYKLLLPLTAFAGASFLIWVDLVCRSINPPVETPISAVISIIGLPCFLWL
                     IRKEKHF"
     gene            complement(7066..>7878)
                     /locus_tag="AC1PNG_001455"
     CDS             complement(7066..>7878)
                     /locus_tag="AC1PNG_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP6701954.1"
                     /translation="ITYHGDIPKNPKKVVNFAYSYTGYLLQLGIDVSSYSLDLEKNSP
                     VFGDKLKDVPQLTTADTEAIAAQKPDVILVFAGDDNLETLKEIAPVIEITYGKSDYLK
                     MLTDIGQIFGKEKEAQAWLDQWDKKVATAKEELKGLIDTSSTFTVMDFFDKNIFLYGN
                     NFGRGGELIYRALGFAAPAKVQEDIINKEGWFGVSQEALPEYVGDYVLVNVNDKTKEA
                     AASLKESDIWKNLPAVKNGHVIEIDYNLFYFSDPMSLDLQIDAFVSEIQKLQ"
BASE COUNT         2477 a         1510 c         1709 g         2182 t
ORIGIN      
        1 aagatgtatc tgaaatgatg gatgaagacc tatttgatgc gggtgttttg gatagtatgg
       61 gaactgtaga acttattgtg gagttagaaa gtcgttttaa tattacagtt cctgtttcag
      121 agtttggtcg tgaagattgg aatacagcta acaaaatcat ttctggtgtt gtggagttga
      181 tgcatgcgta agcaattgtg gcagattttt gggcctgtga tttgtgcaac gtttttagtt
      241 attctcacgt tgttgttttt cccgagttct tctcggtcaa atttcaaaga agaaaaacaa
      301 gatgcagtag cattgtctta tattagtttt aagagtaaat ataagaaaaa tagggctctg
      361 ggagacaaaa atcatcgttt tgttcctttc tttgggtcaa gtgaatggtc acgttttgat
      421 agtatgcatc cgtcagtgtt ggcggatgca tatcagagag agtatacccc ctatttgtta
      481 ggacaacgtg gtgcagcttt gctgacacaa tattttggca tgcaacagat tggggcacat
      541 ttaaaaaata gtcaggctat ctatgttgtt tctcctcaat ggttttcacc caaaggggca
      601 tcatctaagg catttgaaca atattttagt agtgatcaag ctactcattt tctgcaaaca
      661 agtgctgggg atgactatga tagatatgca gcaaagcgtt tcttagagct taaatcagac
      721 tgtagtttga caggaatgtt tcaaaaagta gctagtggta aggaattgtc gaaatgggat
      781 agagagcagt tgacctggca agcctattta ttagaaaagc aagatgcctt atttagtcgc
      841 tttgctgatt ctggtagtaa tttaggattg gtagaaacgg aagcgatgaa acttccagag
      901 actttttcct atgaccgctt gagtaaagtg gctaatgagg acgggagtcg ttctactcaa
      961 tcaaatgatt tccaaattga tgataggttt tatagaaatc gtcttcagtc aaaaatcaaa
     1021 aaactcaaac aatcccagac aaaaatatct tatctgcaat caccggagta caatgatttt
     1081 cagctggttt tgaatgaatt tgctaaacat cagactgatg tgctatttgt aattcccccg
     1141 gttaatgaac gttggatgaa ctacactggt ctcaataaag atatgtatcg ggcaacagta
     1201 gcaaagatta agtaccagtt gaccagtcaa ggtttcaatc atattgctga tttgtcggag
     1261 caggggggga tagattattt tatgcaagat acaatccaca tcggttggca aggctggctg
     1321 gagcttgata agtatatcca cccattcttg acagaacaac aagctcctgt tcattatcag
     1381 ctaaaaaaag aatttctaac ttccgaatgg gctacatata agggagattt aaacctattt
     1441 ttgaaatagt tagatacagt tataataaaa agcaagaagg ctttgaatta ggaagtctcc
     1501 ttgctttttt gttcttttac tgtaataaac tgaatgatta tatatcttct cccaacaaac
     1561 tgtggttggc tttgtggaag cgaataacat gataggcaat catagcgtgt gagagcaggt
     1621 ttaagataac aaatagtagg atatttgaat gaccatccag caataaggca acatttccaa
     1681 ttagtaaaat gagagcatag atgaacagcc ctctcctgcc aacttgatgg acttgttgat
     1741 tatagaagga ggtcgaaagg ataccactat agaagagaag gtttcctaca atcagcatgg
     1801 gcaaggccaa atcattatga tgactaggga aaagctccat tgccactgtg aagaaattca
     1861 agccaagaac aattaccaag tgagcgtata gcagaacagt cgcatctgcc ttgcggtgat
     1921 ggtcgatagt gagataggtc tgagaaatat agaagatgaa cagagatgcc attgccaaga
     1981 agaggaggat tccttccaaa ggaagttcta gaatcggata atttttcaaa atcataatga
     2041 ccgtctcacc aaaggtgata atagtaatca gctggcagcg ttctacgata tgaggaaagt
     2101 tcatcacatc atggctgagt ttgtctttga aaaaatagga aatgaaaaat ggaagataaa
     2161 aaagcaagag gctcttttca tcataggtcc agaaattgaa gtaaatagct accgaagcca
     2221 agaaaaatac tagcacataa atcccaaaca tatagagact atgcttaata tcagccgtaa
     2281 aaccgatttt tcgaccccga aggcagataa gacagggtca aaaagatagt aaagggtagg
     2341 gctgtcgctt taaaatcatg actaaagttt agggataatt gccccaccac aaacatagaa
     2401 gcgatgatag tgaaaatatc cagcaaatct cgctcaccat atttgttgag gtaaatcgtc
     2461 tcattaatcc acaaggtcat aataatcagg ttagcggtga taaaagttag aatttccctg
     2521 agtccgatat ggtccccgtg taagaggcca gtcagtgaag aggtagctaa tacaaagaca
     2581 aatcataaaa taattcatag ttggaaacac gttttgctgt aatttcagac ataaaaatct
     2641 ccttttcaaa aaaatctgac ctgttatacc atacgaaaag ggaggctgtc atctcccttt
     2701 atttcttaca atgtccagtc caagtctggt ccgactggga caatacctgt tgggttaatg
     2761 gttttgtggc taccgtagta gtgctgtttg atatggtcaa agtcaaccgt ctccgcaata
     2821 ccagagtggt tgtaaatccg cttggtataa tcccacaagt ttggataatc aaccaaggcc
     2881 ttgatattgc atttgaaatg cccaaagtaa acaggatcga atcgtaccaa agtggtaaag
     2941 aggcgaatat cagcctcggt caattgattg cccaccagat agtccttgtc tgccaaatgc
     3001 tcttccaatt tatctaaggc ggcaaaaagg ttcttgacct ctttttcata gactgcctga
     3061 ctggttgaaa aacctgcctt gtagactccg ttgttgatgt ttggatagat aaaatcattc
     3121 atggcatcaa tctcggcttg caggtttgct ggataatagt catcgtaatt tccagtaata
     3181 tcattaaaag cagagttaaa catgcgcatg atttcagccg attcattgct gacgatagta
     3241 tttgtttctt tgtcccaaag gacaggcacg gtcacccgac cagaatagtg tgaatcagcc
     3301 ttcaaataga cctgatagag ataatcgctg ttaaagaggg tatccttgat aacaccagga
     3361 ccatcttcaa atgtccagcc attttctaac atgaggggat gaacgacaga caaggaaata
     3421 tggtcttcca agcccttcaa ttttcgcata atcaaggtcc gactagccca aggacaagcc
     3481 agagaaatat agaggtggta gcgaccagac tcagccttga aaccgccctc tcctgttgga
     3541 cctgcctgtc cgtctggcgt aatccagttg cgaaattgtg tgacgctacg aataaactta
     3601 ccgcctgttg acttggtgtc ataccattga tccacccatt tgccatcttg taaaagtccc
     3661 atgaattttc tcccttactg aaaaaatggg aaatccaata ggatgattgg atttcctttg
     3721 taaaaaaata tatcactaac tagtactata aacttttttc aaaaagagtc aagctatatg
     3781 atttgtgata caatagtccc atgagattag acaaatgttt agagaaagct aaagttggct
     3841 cgcgtaagca agttaaaaaa cttttcaaag cccagcaaat caagattaac ggacaagcag
     3901 ctcaaagcct aagccagatt gttgacccag agttacaaac tatccaagtt tcgggtaaaa
     3961 aagttgcctt agaaggttcc gcctattatt tgctccataa accagcagga gtcgtttctg
     4021 cagtgacaga ccaggagcat cagacagtca tcgacttgat ttctccgcaa gacagtaggg
     4081 aaggtctcta cccagtcggt cgcctggaca gggacaccga aggcctggtc ctcattacca
     4141 acaatggtcc gctcggttat cgcatgctcc atcccagcca ccatgtggac aagttctact
     4201 atgtggaggt aaatggatgc ttggctgagg atgcttcaaa attttttgcc tcaggtgtta
     4261 ctttcttgga tggaacacgg tgtcagcctg cagttttgac tgtcttggag gcttctttgg
     4321 accatagcag agcgaccatc aagctggcag aaggtaagtt tcatcaggtc aaaaagatgt
     4381 ttttagccta tggtgtcaag gtcacttatc tcaagcggat ttcctttggc ggttttgagt
     4441 tgggggattt ggaacgaggt gcatacagac aactcacacc aaatgaaatg gaacatttat
     4501 tcacttattt tgactagagg aggatatata atgagcattt atgattttac agtacaaaaa
     4561 caagatggaa cagatcagtc catagctgag tatcaaggac aagttttact tatagtcaat
     4621 acagcgccag gttgtggatt ggcaccacaa tacaaggaac tacaggagct atatgatagc
     4681 tatcgaggcc aaggttttgt agtgttggat ttcccttgta atcaattttt aaatcaggct
     4741 cccggctctg cggaagacat caatcagacc tgtagcttga attatggtac aacttttcct
     4801 cgttttgcta aaattgctgt gaatggatct gaagctagtc cgctgtatcg ctatcttaag
     4861 aaagaaaaat caaccttatt aggaggtcga attgagtgga attttaccaa gtttttggtg
     4921 gatagacaag ggcgtgtggt caaacgatat ttaccaacaa ctagtccatt aaaacttaaa
     4981 gaggatattg aattttacct agaaaaataa caagaaggct ggagtcatct agccttcttg
     5041 ttttagtaac cacagaaaat agggagcgcc gataagggag acgatgatgc cggttgggat
     5101 gcctgttccg acaagtagta cccgtcctat ggtatccgct agaagtagaa tgaagcttcc
     5161 tattatcata gtagctggaa tgatgacacg atggtcattt cctaaccatt tgcgcacaat
     5221 gtgaccagca atgagaccaa cgaaggtgat attaccaact aaaatgacac taagagaggc
     5281 tagggcggta gccaatatca aggtaatccg cctttcacgc tgtaagtgga gtcccaaagc
     5341 catggctgtc tggtcgttta gattcataat attgagtgag cgacttcgac taaacgttgc
     5401 catccatacc aatagaagaa gaggagtata gattgcgata gttttccaat ctccaccatt
     5461 tactcgaccg ctgagccact ggacaatgta ttccatctta aagtcatcta attggctgat
     5521 gatgctgacc atgataccag acagcatgct cgaaattcca actcccgtga taattaagcg
     5581 tgcaggatgg atgccgtaca gcttcttacg tgcaatccag taaactagaa aaatggttaa
     5641 tccaccgccc atcatagcaa atagaggcat gagactcaat aagacagggc tgatttttga
     5701 agagagacca acaaatagtg ccacaacaag accagctcct gcgttgattc ctaatattcc
     5761 agaatctgcc agaggattgc gggtcaaagt ttgtagcaac aaacccgcca aagccagaga
     5821 agcccctccg ataatggagg cgattattct aggtaagcga attcgcccaa taatcagtag
     5881 agtcgatgaa ctggcacgcc cactcatgat gtcgatgaga tctgaaaagg atgaattgct
     5941 atagccgata gataaggaaa gtaggaaaat gacaaataaa aggatcagca ggaatgtgaa
     6001 tatttttaga tagttgtgat gtcttatcaa aagtgtttct cctttctaat caaccatagg
     6061 aagcaaggga gaccaatgat actgatgact gcgctaatgg gagtttcaac aggtggattg
     6121 atagaacggc aaaccaaatc tacccagatg aggaaactag cccctgcaaa ggcagttaat
     6181 ggcaagagta gcttgtaatt tttccctgta aacatgcgga taaagtgagg aataatgagc
     6241 ccgataaaag aaagcgagcc cacaagagct accgcagaag ctgagagtag aaggacaatt
     6301 cccaacaaaa ccaaggtcat cagtaaggtt cgttgaccta gatttttagc aacggtttcg
     6361 ttgagactca ggatggtcag ctgatgcgaa aagagttgag caaggatgag gccgatgatg
     6421 ataagaggag caatgatggc cagcatcttc cagtttacct gtaccaatcc ccctgcttgc
     6481 cagccaatta ctgcagttga taaatcaaag tagatagtaa tagcctgacc aatagcagaa
     6541 aatagacttg ctaccatggc gccagataaa attaaacgga gttgattgta gcctttttgc
     6601 acctgataag ctagaccaaa gactagaccg gctgccagac aggagcccag taaacatatt
     6661 aataaaattt gactgtagtg caaaccaccg aacagagcat aggcaacaat gagagcaagt
     6721 cctgctccag cattgattcc taataagcca ggatcggcaa tcggattgcg tgtaattcct
     6781 tgcatcattg ccccagcttg ggccattgca gctcccacca agatggcggc taccattcta
     6841 ggcaagcgca aatcgatgat aacatcctga gcggaagaat ctgtcagtgg atgctttagg
     6901 gtttctatca gttgttggtg gctataagaa atagctccaa agcgtaaact aagatagact
     6961 ccagctatga aagaaagaca gatgatgaaa aaaacaagcc aaatattctt tggctttgtt
     7021 ttaggataac gagaacttac tagtttgttc atatatccag tccttttact gaagtttttg
     7081 aatttctgag acaaaggcgt caatttgtaa atctaaagac atcggatcgg agaagtagaa
     7141 gaggttatag tctatctcga tgacgtgacc attctttaca gcaggtagat ttttccaaat
     7201 atcactttct ttgagagagg cagctgcttc ttttgtttta tcattgacgt taacaaggac
     7261 atagtcgcca acatattctg gaagagcttc ttgagatacc ccaaaccagc cttctttatt
     7321 gatgatgtct tcttgaacct ttgcaggagc ggcaaaaccg agagcgcgat agattaattc
     7381 tccaccacga ccaaagttat taccataaag gaagatgttc ttatcgaaaa agtccatgac
     7441 ggtaaatgtt gaacttgtat caatcaatcc tttcaattcc tctttcgcag tagcaacttt
     7501 cttatcccat tgatctaacc aggcttgtgc ttccttttct tttccaaaaa tctgaccaat
     7561 atctgtcagc atttttaagt agtcactctt tccataagtg atttcaataa ctggagcaat
     7621 ttctttcagt gtttctaagt tatcatcacc agcaaatact agaatgacat ctggcttttg
     7681 tgcggcaatc gcttctgtat ctgccgttgt caattgtgga acatccttta gtttgtctcc
     7741 aaagacggga ctatttttct ctaaatctag actatagcta gatacatcaa ttcctaattg
     7801 taagagatag cctgtgtaag agtaagcaaa gttgacaact tttttaggat tttttggaat
     7861 gtcaccgtgg taagtaat
//
LOCUS       DBNFEG010000104         7853 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.104, whole genome shotgun sequence.
ACCESSION   DBNFEG010000104 DBNFEG010000000
VERSION     DBNFEG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.104"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(161..436)
                     /locus_tag="AC1PNG_001456"
     CDS             complement(161..436)
                     /locus_tag="AC1PNG_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701955.1"
                     /translation="MITDQDYRWIADDVYKVDPLKTDDTLKQGGRAGQGQYVIIEPPV
                     DAGNGMQAMAVAPIKGYDSDNKPIPDTSQVVIASFPYPDSSVNTEYL"
     gene            complement(436..846)
                     /locus_tag="AC1PNG_001457"
     CDS             complement(436..846)
                     /locus_tag="AC1PNG_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701956.1"
                     /translation="MSPKRTRLILVMSAICIAIGGMIMFSFHRMSEEEKLQAQIRKEQ
                     ERMVLYAVNHYEGIEKIEFTSFRENRMTGAWYAGAILNDDYKVTITAMGFDGDLSMND
                     GPSDKTGLYLRIKDKPTTISNPNHIEVIYFKGDN"
     gene            complement(924..1157)
                     /locus_tag="AC1PNG_001458"
     CDS             complement(924..1157)
                     /locus_tag="AC1PNG_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701957.1"
                     /translation="MITVQDYRWIAEDVYKVDPLKTDDTFKDGDRVAQDKFVILSQPQ
                     DMINGMQAMAVAPIKGYDAENKPIPDTSQVVIA"
     gene            complement(1154..1498)
                     /locus_tag="AC1PNG_001459"
     CDS             complement(1154..1498)
                     /locus_tag="AC1PNG_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701958.1"
                     /translation="MFNFHRMSEEEKLQAQIRKEQERMVLYAVNHYEGIEKIEFTSFE
                     ENKMTGSWSAGAILNDDYKVTITAMGFDGDLSMTNGPSDKTGLYLRIKDKPTTISIPN
                     QIEVIYFEGDNQ"
     gene            complement(1690..2631)
                     /gene="msrB"
                     /locus_tag="AC1PNG_001460"
     CDS             complement(1690..2631)
                     /gene="msrB"
                     /locus_tag="AC1PNG_001460"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIP6701959.1"
                     /translation="MKEIYLAGGCFWGMEGYFSQIDGILDTSVGYANGQVETTNYQLL
                     KQTDHAETLYLAYDETRINLREILLYYFRVIDPFSVNQQGPDKGRQYRTGIYYTDKAD
                     LPTIEQVMMEQSQLFGGRPLAVEVEPLAHYIPAEDYHQDYLKKNPQGYCHIDLGQAKI
                     PLIDVADYQKPDQQVLKDSLTDLQYQVTQEAATERPFENEFWNSDQAGIYVDITTGEP
                     LFLSTDKFDLGCGWPSFTKPISKEVATYFQDKSHGMNRIEVRSRAGHAHLGHVFDDGP
                     RDKGGLRYCINSAALRFIPREEMEAAGYGLFLDLLSE"
     gene            complement(2671..4200)
                     /locus_tag="AC1PNG_001461"
     CDS             complement(2671..4200)
                     /locus_tag="AC1PNG_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIP6701960.1"
                     /translation="MSTISYKPLNLYRQFKTAAQEFPNVAIYFDKPYASFPELGLENT
                     YQTVFEAIQKRAAQFSAAGIGYGDKVILYKSPAFDTYLLAVAVTALGAVPVMISYHLP
                     SSNLDVFAERLEKSFIVYDQETEERVAGMTRTDLVTKVFLPQVLVQEAVAFEENLLPE
                     DVIQYMTHTSGTTGVPKLICHTAQTMGWRVAWQQTIFDKMTERGLLAFHISPVHSRYN
                     IGVSSAIGLGFPLYPLSSARKDDVERALALHRPSALETHPNNFVQWARLAKEKPEVFS
                     SIRYYHSTFDAINIGTLRAFLEASKEQHPVFMQVYGQSECGPMILRYHRLENLGTVSG
                     RDMGIGLEGYTEARITDAQGNPLPAGENGHIQFLSKGRAVTYYKEDARFQDNVYGAWW
                     DSGDYGCMTPEGTLLLKDRQVDLIEHIDSNLALEDLLLDKLDFLSEVVIIRDVNGAPQ
                     PIIALVEDAEMNWEAWWAMVADLPLLKEPILMAYDDIPRTATMKVQRLKMEAEMKEKN
                     L"
     gene            complement(4333..4647)
                     /gene="trxA"
                     /locus_tag="AC1PNG_001462"
     CDS             complement(4333..4647)
                     /gene="trxA"
                     /locus_tag="AC1PNG_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIP6701961.1"
                     /translation="MVQVITDANFEVETQEGVVLVDFWAPWCGPCRMQAPILEQLADE
                     VDEDELRIYKMDVDENPNTARQFGIMSIPTLLFKKDGQVVKQVAGVHTKNQIKAILAE
                     IG"
     gene            complement(4887..5207)
                     /locus_tag="AC1PNG_001463"
     CDS             complement(4887..5207)
                     /locus_tag="AC1PNG_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701962.1"
                     /translation="MHQLYQMLQDLYTKADQIDYATINAIEKEIERWLIRSIYAPLKL
                     REKNAILKQHKKKIQTIGNDGENSVKLIANSFDTSIKGEFSKKVQQVVKEKAKEYTKL
                     EEVE"
     gene            complement(5220..5606)
                     /locus_tag="AC1PNG_001464"
     CDS             complement(5220..5606)
                     /locus_tag="AC1PNG_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701963.1"
                     /translation="MDQYDKLKAELMKKEWEWEDIENQQRKAQKELQEHYENVEETTR
                     ILTRMLEEKYQEVLLELRQVGDETGDLHHLLNNGMSEWHTAIDQERYSSIHRLDQKQE
                     DLDTYYKNQYRKMQDQIDEIYTKYRE"
     gene            complement(5585..6802)
                     /locus_tag="AC1PNG_001465"
     CDS             complement(5585..6802)
                     /locus_tag="AC1PNG_001465"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIP6701964.1"
                     /translation="MTEYNNLGKGDAVITQYDDIVGRVTLDFDNENGNGEQVYAVVKD
                     PNLPKDQVLEVTVLFRGSTGPNEVLSKPADVWNDWVENDLFLGLRVFAQDHPNYTQNH
                     DHASGQLKASSEALNTIMATYPNAKVNLYGHSLGSMDVQYAIANLSDTDINRLESAYI
                     YNGPNIYGILDDQQKARVDSVKGLIHNYADPKDPVSMVGRDLDKGSLDSVGMVHFVES
                     KDIDLGNQHMTYGYQLDSSGNIKVLQTSSTEGINGTIIEMSRFQQMKQTLSRGGFSSR
                     ETIYLDSEQARILAQGLVKVAETTHQTLEKETTSTLTEVNEVYSSLGNVPFGFILSPD
                     EVRQAYSSAGVDYHSLVGDSTNQVEKFITRSNQLKQDLVDLESQIQAGIEQKVTEDQT
                     LAQRIQEWTSTIN"
     gene            complement(6849..7226)
                     /locus_tag="AC1PNG_001466"
     CDS             complement(6849..7226)
                     /locus_tag="AC1PNG_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 family protein"
                     /protein_id="HIP6701965.1"
                     /translation="MKKVMKVIIGILLVIGIGIGGFVYMQHKEHEKMVAIATSEEARA
                     VYEKHMKANDPKALTADGIIKTYEIDTETLEYNPMGGMIVRVYVNGDEDFGFHFNLLQ
                     NEDGKFKSGSYVIKSKLADLLGK"
     gene            complement(7252..7515)
                     /locus_tag="AC1PNG_001467"
     CDS             complement(7252..7515)
                     /locus_tag="AC1PNG_001467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701966.1"
                     /translation="MTEKYTDRDRQKIANKEYSAYKFGDEVNIGTLEHPRYIGTVRQV
                     LKDATGLDGYVVEEPDGNECCDGDGNTVSAASMLYYEVRKHLT"
     gene            complement(7508..7852)
                     /locus_tag="AC1PNG_001468"
     CDS             complement(7508..7852)
                     /locus_tag="AC1PNG_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 family protein"
                     /protein_id="HIP6701967.1"
                     /translation="MGIGGIKLMQKIEHDQMVEIVKSEEVREIVEEQLKYLDNEALSE
                     DGLIKTYEIDDSSIIHSPMGGISFNVFLNSDRKLRVVFELEKDSNTGKIEYSGGGYSS
                     EVKKLVGEINND"
BASE COUNT         2067 a         1789 c         1477 g         2520 t
ORIGIN      
        1 ctcttgttcc tttcggattt gagcctgcaa tttttcttcc tcactcatac ggtgaaaact
       61 aaacattatc attcctccta ttgcgataca aatggcactc ataaccagta tgaggcgggg
      121 acattttttg gacatataaa acctccttaa gttgattata ctataaatac tcggtattta
      181 cactagaatc aggataagga aaactggcaa taacgacttg ggaagtatct gggatcggtt
      241 tgttgtcaga gtcatagccc ttaatcggag caaccgccat ggcctgcata ccgttgcccg
      301 catcaacggg tggctcgatg atgacatact gcccttgtcc tgctctaccg ccttgtttta
      361 aagtgtcatc tgttttcaac ggatctactt tatatacatc atctgcaatc cagcgataat
      421 cctgatctgt tatcattagt tatccccctt aaaatagatt acctcaatgt gatttggatt
      481 tgaaatggtt gtcggcttgt ctttaattcg taaatacaaa cctgttttgt ctgaaggacc
      541 atcattcatc gacaaatctc catcaaatcc cattgctgtt atcgtaactt tataatcatc
      601 atttaatata gcacctgcat accaagcacc tgtcatccta ttttctctaa aactggtaaa
      661 ctcgatcttc tcaatccctt catagtgatt gacggcatac aagaccatac gctcctgttc
      721 cttacggatt tgagcctgca acttctcttc ctcactcatg cgatgaaaac taaacattat
      781 cattcctcct attgcgatac aaatggcact cataaccagt atgaggcggg tacgttttgg
      841 ggacatataa aacctcctta tgttgattat actataaata ctcggtattt acactagaat
      901 caagataaga aaactatctt atactaggca ataacgactt gggaagtgtc tggaatcggt
      961 ttgttttcag catcgtagcc cttaatcgga gcaaccgcca tagcctgcat accattgatc
     1021 atatcttggg gctgggagag gatgacgaat ttatcttgtg cgactcggtc cccatcctta
     1081 aaagtgtcat ctgttttcaa cgggtctact ttatatacat cctcagcaat ccagcgataa
     1141 tcctgaactg ttatcattgg ttatccccct caaaatagat tacctcaatt tgatttggaa
     1201 ttgaaatggt tgtcggcttg tccttaattc gtaaatacaa acctgttttg tctgaaggac
     1261 catttgtcat cgacaaatct ccgtcaaatc ccattgctgt tatcgtaact ttataatcgt
     1321 catttaatat agcacctgca gaccaagatc ctgtcatctt attttcttca aagctggtaa
     1381 actcgatctt ctcaatccct tcatagtgat tgacggcata caagaccatc cgctcttgtt
     1441 cctttcggat ttgagcctgc aacttttctt cctcactcat gcggtgaaaa ttaaacatca
     1501 tcattcctcc tattgacagg aacaacaaac cggtgaataa aactagtcgt tttttgcata
     1561 caaacataaa aatcctccta atataattat attattcttg aacataactt ttcaaataaa
     1621 aaggataata aagagcttaa gttcaatacc ttaagctctt taatctattt actttgaagt
     1681 gtttccctgt cattctgaca gcaaatccaa aaacaagcca tatcctgctg cctccatttc
     1741 ctctctcggt atgaagcgaa gggctgcgga gttgatacag taacggagac cgcctttgtc
     1801 acgtggtccg tcatcaaaga catgacctaa atgagcatga ccagcccgac tgcggacttc
     1861 aatacggttc atgccgtggg acttatcttg gaaataagtc gccacttcct tgctgattgg
     1921 tttggtaaag gagggccagc cgcagcctaa atcgaatttg tctgtcgaga gaaagagggg
     1981 ctcaccagtc gtaatatcga cgtagatgcc agcttggtcg ctattccaaa actcattttc
     2041 gaagggtctt tccgtcgcag cttcttgagt gacttgatac tggaggtcgg tcaagctgtc
     2101 tttcaagact tgctggtctg gcttttggta gtctgcaaca tcaatcagag ggatttttgc
     2161 ctgcccaaga tcgatatggc agtacccttg gggatttttc ttgagataat cctgatggta
     2221 atcttcagcg gggatgtaat gtgcgagtgg ctccacttca acggctaggg ggcgtccgcc
     2281 aaagagctgg gactgctcca tcatgacctg ctcgatggtc ggcaaatcag ccttatctgt
     2341 gtaatagata ccagtccgat actggcgacc tttgtcaggt ccctgctgat tgacagagaa
     2401 gggatcaatg acgcggaaat aatagaggag aatttcccgc aaattgatac gagtctcatc
     2461 ataggctaga tagagtgtct ctgcgtggtc tgtttgtttg aggagttgat aattggtcgt
     2521 ctccacctgt ccattggcat agccaacgct ggtgtcaaga ataccatcga tctgggaaaa
     2581 gtagccttcc atcccccaga aacaaccgcc tgctaggtag atttctttca tgtgaactcc
     2641 ttcttatctg caagcgtggt ttcgtcaccc ttacagattt ttttctttca tttctgcttc
     2701 cattttgagg cgttgaacct tcatggtcgc tgttcgtgga atatcgtcat aagccatcaa
     2761 gataggctct ttcaatagtg gtaggtctgc aaccatggcc caccaagctt cccaattcat
     2821 ctcagcatct tctaccagag cgataatcgg ctgcggtgca ccattgacat cgcggataat
     2881 cacgacttct gataaaaagt ccaatttatc cagcaataag tcttccaagg caaggttgct
     2941 gtcgatgtgc tcaatgaggt caacctgacg gtcttttaga agaagcgtcc cctctggagt
     3001 catgcagccg tagtcgccac tatcccacca agcaccgtac acattatctt ggaagcgggc
     3061 atcttctttg tagtaagtca cagcacggcc ttttgacagg aactggatat gtccgttttc
     3121 tccagctggg agaggattcc cttgggcatc tgtgatgcgt gcttctgtgt aaccttccag
     3181 accaatcccc atatctcgtc cgctaactgt tcctaggttt tccaagcgat ggtaacgtaa
     3241 aatcattgga ccacattcac tttgaccata aacctgcata aagactggat gctgctcttt
     3301 agaagcctcc aagaaggcgc gaagcgtacc aatattgata gcatcaaagg tcgaatggta
     3361 ataacgaatg ctactaaaga cctctggttt ttccttggcc aatcttgccc attggacaaa
     3421 gttattcgga tgggtttcca gagcacttgg acggtgaaga gcaagcgccc gttcaacatc
     3481 atcttttctt gcagaggaaa gcgggtagag ggggaatcct agcccaatcg ccgacgacac
     3541 gccgatatta tagcgtgaat gtacagggga gatatggaag gccaacaagc ctctctccgt
     3601 catcttgtca aaaatagttt gttgccaagc tactcgccag cccatggtct gtgctgtgtg
     3661 gcagattagt tttggaacac ctgtcgtccc tgaagtatga gtcatgtatt gaataacatc
     3721 ttctggcagg agattttctt caaaagcaac tgcttcttgc actaggactt ggggtaaaaa
     3781 tacctttgtt accaggtcag ttcgagtcat tcctgctaca cgttcttccg tttcttggtc
     3841 gtagacaatg aaagactttt ccaatcgctc cgcaaacaca tctaaattcg aagatggcaa
     3901 atggtaggaa atcataacag gaacagcacc caatgccgtt accgctactg ctaacaaata
     3961 ggtgtcgaaa gctggactct tatatagaat taccttgtcc ccatagccaa tacctgctgc
     4021 tgaaaattgg gcagctcttt tttgaatagc ttcaaaaact gtttgatagg tattttccaa
     4081 tcctaattca gggaaagatg cgtagggttt gtcaaaataa atggcaacat ttggaaactc
     4141 ttgagctgct gttttaaact gacgatagag atttaatggt ttgtaactaa tagttgacat
     4201 atctaacctt tctaaaatga caatgataag gttattataa catcttgtgc caaccttcac
     4261 aatctgatat ctttttatga ctcacagttg tgaaaaaaca agtagtcaat caccacttgc
     4321 tttcattttc aattaaccaa tctctgccaa gatagctttg atttgatttt tcgtgtggac
     4381 accagcaact tgtttcacaa cctgtccatc ttttttgaac aaaagagttg ggatagacat
     4441 gataccaaac tgacgagctg tgtttggatt ctcatcaaca tccatcttgt aaatacgcaa
     4501 ttcatcttcg tccacttcgt ctgctaattg ttccaagatc ggtgcttgca tacggcatgg
     4561 accacaccat ggtgcccaaa aatctacaag gactacgcct tcttgtgttt caacttcaaa
     4621 gtttgcatct gtaataactt gaaccataat tttctccgtt tctgtttttc tactatcatt
     4681 ctacaccaaa aaaaaacaga aagcgaattt ttgctacggg atgaagcatg agataaacgt
     4741 atctataaga atttcagttg taaaaaaatg ggtatagcta aactagaccg caggaggacg
     4801 agggggaact gggagactgt tcttcctata aaatccagtt ttagcaatgg gaaaagattt
     4861 acgaaaggat gctacgggat gagatattac tctacctctt ccaattttgt atattctttt
     4921 gccttttctt tcacaacttg ttgtaccttt tttgaaaact cgccttttat acttgtatca
     4981 aaactgtttg caatcaattt tacggaattt tctccatcat ttccaattgt ttgaattttc
     5041 ttcttgtgtt gttttaaaat tgcatttttt tctcgcagtt tgagtggagc atagatagag
     5101 cgaatcagcc aacgttcaat ttctttctct atagcattaa tcgtcgcata gtctatctgg
     5161 tcagctttgg tatataaatc ttgcaacatt tgataaagtt gatgcattgg ctctcccttc
     5221 tattctcggt attttgtata aatttcgtca atctgatctt gcatttttcg gtactggttt
     5281 ttatagtagg tatccaagtc ctcttgtttc tgatccaatc ggtgaatgga gctgtatcgc
     5341 tcttgatcga ttgcggtatg ccattcggac atgccattgt tgagtaaatg gtgcaagtct
     5401 ccagtctcgt ctccgacttg tcgcaattct aacagaactt cttgatactt ttcttccaac
     5461 attcgagtca atattcgagt ggtttcttca acattttcat agtgttcttg tagttctttt
     5521 tgagcttttc gttgttgatt ctcaatatcc tcccattccc actctttctt cattaactct
     5581 gcctttaatt tatcgtactg gtccattctt gtatcctctg tgctaatgtc tggtcttccg
     5641 ttactttctg ctcgatccct gcttggattt gactttctaa gtccactaaa tcctgcttga
     5701 gttgattgga gcgagtgata aacttctcga cttgattggt gctatctcct accagactat
     5761 gatagtcaac acctgcactg ctgtaggcct gtctcacttc atcaggactc aaaatgaagc
     5821 caaaagggac atttcctaag ctactgtaga cttcatttac ttctgtaagg gtggaagtgg
     5881 tctctttttc gagtgtttgg tgagtggttt ccgcaacctt aaccagtcct tgagccagga
     5941 tgcgggcttg ttcgctgtcc agatagatgg tttcacgcga actgaagcca ccacgggata
     6001 gggtttgttt catctgttgg aagcgactca tttcaatgat ggtcccattt atcccttccg
     6061 tggaagaagt ctgtaggacc ttaatattgc cagaagagtc taactgatag ccataggtca
     6121 tgtgctgatt gccaagatcg atatccttac tttccacgaa gtggaccatc ccaacggaat
     6181 ctaaacttcc tttgtctagg tcacgtccga ccatactgac aggatccttt ggatctgcat
     6241 aattatgaat cagccctttg acggagtcga cccgtgcttt ctgttggtcg tctaggatgc
     6301 cgtatatatt agggccatta tagatatacg cgctctctaa gcggttaata tcggtgtcag
     6361 ataaattagc aatggcgtac tggacatcca tcgaacctag ggagtggccg tacagattga
     6421 cttttgcatt tgggtaggta gccattattg tatttagagc ttctgaggat gcttttaatt
     6481 gtccactcgc atggtcatga ttctgggtat agttcggatg atcttgagca aagacgcgca
     6541 aacctaaaaa aagatcgttt tcaacccagt cattccacac atctgcaggt tttgacaaca
     6601 cttcatttgg tccagtcgag cctcgaaaga gaaccgtaac ttccaatact tggtctttgg
     6661 gcaggtttgg atctttcaca acagcataaa cctgttctcc attgccattc tcattgtcga
     6721 agtcgagggt gacacgacct actatgtcat catattgtgt aattacagca tctcccttac
     6781 ctaaattatt gtattccgtc attgcaatct gaagattatc ttcattaaca tatttttttt
     6841 accatgaact acttccctaa taaatctgcc aattttgatt tgataacata acttccagac
     6901 ttaaactttc catcttcatt ttgtaaaagg ttaaaatgaa agccaaagtc ttcatctcca
     6961 tttacataaa ctcttacaat cattcctccc atgggattat actctagtgt ttcagtgtca
     7021 atttcataag tttttataat tccatcagcc gttaatgctt ttgggtcatt cgctttcatg
     7081 tgtttttcgt aaacagctct agcctcctcg ctagtagcga tagcgaccat tttctcatgt
     7141 tccttgtgtt gcatatatac aaatcctcct attccaatcc cgattactaa caaaattccg
     7201 ataataactt tcattacttt tttcatccta gacctccttt atgaaatcct atcaagtaag
     7261 atgctttctt acctcatagt ataacataga tgcagctgaa actgtgttac cgtcaccgtc
     7321 acaacactcg ttgccgtcag gctcttcaac aacataacca tccaaacctg ttgcatcttt
     7381 gagtacctga cggactgtgc cgatgtatct agggtgttct agggtcccga tatttacctc
     7441 atctccaaat ttgtaagctg aatactcttt gttggcaatt ttctgtcgat cacgatcagt
     7501 atacttttca gtcattgttt atttctccaa ctaatttttt cacttcagaa gaataccctc
     7561 ccccggagta ttcaattttt cctgtattag aatccttttc taattcgaac accactctta
     7621 actttctatc tgaattcaag aaaacattaa aacttatccc tcccatcgga ctatgaatga
     7681 tagaagaatc atcgatttca tatgttttta tcagcccatc ctctgaaaga gcttcattat
     7741 ctagatattt aagttgttct tctacaatct ccctcacttc ctcactcttc acaatctcca
     7801 ccatctggtc gtgctcaatt ttttgcatca gcttaattcc tcctattcca atc
//
LOCUS       DBNFEG010000105         7835 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.105, whole genome shotgun sequence.
ACCESSION   DBNFEG010000105 DBNFEG010000000
VERSION     DBNFEG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7835
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.105"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            83..862
                     /locus_tag="AC1PNG_001469"
     CDS             83..862
                     /locus_tag="AC1PNG_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6701968.1"
                     /translation="MIISMKNVHYKRQGKTMLEDINWEFKKGERWAILGLNGAGKSTL
                     LRILMAEFWKTSGDLTVLGVEFGKGDIPSLRTKIGVVGSFLAERFPIDLTAEQIVLTG
                     KYKSSILYREYGQAELDEAIDMLKTIKAEHLIGRAYASLSQGERQLLLIARSLMEEPA
                     ILILDEATVGLDLLARERLLHHIDYLCQLPTAPAIIYVTHHAEEITDNFTHVLLLKDG
                     MVLAQGPKEDILTPELLSDFYGNQVELIDLGEGRLFIKPIL"
     gene            complement(878..1432)
                     /locus_tag="AC1PNG_001470"
     CDS             complement(878..1432)
                     /locus_tag="AC1PNG_001470"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (mnm(5)s(2)U34)-methyltransferase"
                     /protein_id="HIP6701969.1"
                     /translation="MLRPLHMAHAFLDEILTDQDLAVDATMGNGHDTLFLAQRAGKVV
                     AFDIQEQALTTTAEKLEKAGLTNAQLVLTGHENLDQYVDECKAAIFNLGYLPSADKSV
                     ITLPATTLQAIEKVLNRLVVGGRLAIMIYYGHEGGALEKDAVLDFISQLDQTVFTAML
                     YKPLNQVNTPPFLVMVERLKSSSN"
     gene            1563..2207
                     /locus_tag="AC1PNG_001471"
     CDS             1563..2207
                     /locus_tag="AC1PNG_001471"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIP6701970.1"
                     /translation="MKTIILFFLGASIGSFLGLVIDRFPEQSIIAPSSHCNACKRRLK
                     AWDLIPVLSQLSTKSKCRYCKAKIPYWYLGLEFLAGLVVLLCHFQVLNLTETILILAG
                     LVLTIYDIKHQEYPFAVWLIFTFIALILSQLNWLFCGFLLLAYLTEKWQINIGSGDFL
                     YLASLALICGFTELLWIIQISSLLGLLVFAIFKPKSIPYVPLLFLSSIPIILCI"
     gene            2315..3775
                     /locus_tag="AC1PNG_001472"
     CDS             2315..3775
                     /locus_tag="AC1PNG_001472"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIP6701971.1"
                     /translation="MTLFKDDSLTLHTDLYQINMMQVYFDQNIHNKHAVFEVYFRSQP
                     FKNGYAVFAGLERVVNYLNNLSFSQTDIDYLTELGYPQDFLDYLSQLEMTLTVRSAKE
                     GDLVYANEPILQIEGPLAQCQLIETALLNIVNYQTLIATKARRIKAVIEDEPLMEFGT
                     RRAQEMDAAIWGTRAAFIGGASATSNVRAGKIFGIPVAGTHAHALVQTYGDDYKAFKA
                     YAETHHNCVFLVDTYDTLRIGVPAAIKVAKEMGDKINFKGVRIDSGDMAYISKKVRQQ
                     LDDAGFTDAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVS
                     IEDEDGNMRDTIKLSSNAEKVSTPGKKQVWRITSKEKGKSEGDYITFADTDVTQMDNI
                     YMFHPTYTYINKTIENFDARPLLVPIFEKGKQVYELPSLAEIQTYANQEFDKLWDEYK
                     RVLNPQLYPVDLAQDVWDNKMNLINRIRKQTQTKSI"
     gene            3788..4612
                     /gene="nadE"
                     /locus_tag="AC1PNG_001473"
     CDS             3788..4612
                     /gene="nadE"
                     /locus_tag="AC1PNG_001473"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HIP6701972.1"
                     /translation="MTLQETIIKELGVKPSIDPKEEIRRSIDFLKDYLKKHPFLKTYV
                     LGISGGQDSTLAGRLAQLTMEEMRAETGDDSYQFIAIRLPYGVQADESDAQAALAFIQ
                     PDVSLTINIKESTDAMVAAVNANGLAMSDFNKGNAKARMRMIAQYAIAGERKGAVIGT
                     DHAAENITGFFTKHGDGGADILPLFRLNKRQGKQLLAALGADEKLYLKVPTADLEEDK
                     PGLADEVALGVTYNQIDDYLEGKTIDPQSQTIIEGWWNKTAHKRHLPITIFDDFWK"
     gene            complement(5486..6013)
                     /locus_tag="AC1PNG_001474"
     CDS             complement(5486..6013)
                     /locus_tag="AC1PNG_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIP6701973.1"
                     /translation="MRKFFQADGNLTMLGRQICKTLAGAYALAIVLLCFLPQTWYPQY
                     KDFSTPGIIQIGRLYLLPTPFNSIVNGDKVDSLADLGWIFLQNITNIFLLFPLIFLLL
                     FLREEWRSLRAVIRYSFCISLFIECTQLLLDLLIDAQRVFEIDDLWTNTMGGILAYFT
                     YSLIMRGIILSKQES"
     gene            complement(6010..7122)
                     /gene="rlmN"
                     /locus_tag="AC1PNG_001475"
     CDS             complement(6010..7122)
                     /gene="rlmN"
                     /locus_tag="AC1PNG_001475"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HIP6701974.1"
                     /translation="MKPSIYAFSQANLVDWILENGEKKFRATQIWEWLYRSRVQSFAE
                     MTNLPKSLIEKLEEHFVVNPLKQRIVQESKDGTIKYLFELPDGMLIETVLMHQHYGLS
                     VCVTTQVGCNIGCTFCASGLIPKQRDLTSGEIVAQIMLVQKYLDERNQNERVSHIVVM
                     GIGEPLDNYDNVMTFLRVVNDDKGLAIGARHITVSTSGLAPKIREFACEGVQVNLAVS
                     LHAPNNDLRSSIMRINRKFPIEVLFEAIEDYIKVTNRRVTFEYIMLNEVNDGVEQAQE
                     LADLTKNIRKLSYINLIPYNPVSEHDQYSRSTKERTLAFFDVLKKNGVNCVVRQEHGT
                     DIDAACGQLRSNTLKKDREKARARIAAAKAKAGIRA"
     gene            complement(7131..7772)
                     /locus_tag="AC1PNG_001476"
     CDS             complement(7131..7772)
                     /locus_tag="AC1PNG_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HIP6701975.1"
                     /translation="MKKEISPEMYNYNKYPGPSFARVGDKVVSENIELDLLEDYKNAF
                     DQTIFGQRFSQLMLKFDYIVGDWGNEQLRLRGFYTDDKNVKSDLKISRLDDYLTEYCN
                     FGCAYFVLANPNPQDLPAEEEDRPRRKRSRSRNRNRNQQRTELIEPKENKSNRNDRQK
                     KGKQQNKQSEQRHFTMKNAGVKASQTERSQKHERKQSKREMNEVKRNFVIRQK"
BASE COUNT         2294 a         1800 c         1508 g         2233 t
ORIGIN      
        1 taaaatttaa catatttctt gctataatta gtccctcatg tctgtttatg ttacaataat
       61 caaaaggaat aggagagcaa ctatgattat ttcgatgaag aatgtccact ataaacgaca
      121 aggtaaaact atgttagaag acatcaactg ggaatttaaa aaaggcgagc gttgggctat
      181 tttaggactc aacggggctg gaaagtcaac tctacttcgc attttaatgg ctgaattttg
      241 gaagacctct ggtgacctga ctgttcttgg agtggagttt ggtaagggag atattcccag
      301 cctacgcacc aagattgggg tagttggctc ctttttggca gagcgttttc caatcgattt
      361 gacggctgag cagattgtct tgacaggaaa atataaatcc tccatcctct atcgtgaata
      421 cggtcaagct gaattggatg aagccataga catgctcaaa accattaagg ctgagcactt
      481 gatcggtcgg gcctatgcta gtttgtcaca aggagaacgc caactcctgc tgattgcccg
      541 aagtttgatg gaagagccgg ctattttgat tttagacgaa gcaactgtcg gtttggacct
      601 cctagctcgc gagcgcctgc tccaccacat tgactacctc tgtcaactcc caacggctcc
      661 tgccattatc tacgtcaccc atcacgccga ggaaatcact gataatttca cccatgtcct
      721 cctgctcaaa gatggcatgg tcttggcaca gggacccaaa gaagatatcc tgacacctga
      781 actcctcagc gatttttatg gaaatcaggt ggaattgatt gacttgggtg agggaagatt
      841 gttcattaag ccgatattat aaaaaacaaa tccagctcta gttcgagctg gattttaatc
      901 gttccaccat gaccaaaaat ggtggggtat taacttggtt gagcggtttg tagagcatgg
      961 ctgtaaagac ggtttggtca agctggctga taaagtctaa aactgcatcc ttctcaagag
     1021 ctccaccctc gtgaccatag taaatcatga tagccaagcg acctccaacc accaatctat
     1081 tcaggacttt ctcaatggct tgaagggtgg ttgcaggcag ggtaatgact gacttgtccg
     1141 ccgaaggtag atagcccaga ttgaaaatcg ctgccttgca ttcatccaca tactggtcta
     1201 aattttcgtg tcctgtcaaa accaattggg cattggttaa gccagctttt tctagttttt
     1261 cagccgttgt ggtcagggct tgttcctgaa tatcaaaggc cacaactttt cctgctcgtt
     1321 gggctaaaaa tagcgtgtca tggccatttc ccatagtcgc atcgaccgct aggtcctggt
     1381 cagttaaaat ctcatctaaa aaggcatggg ccatgtgtaa aggtctaagc atactttctc
     1441 ctaattactg tactttctac tattttgtaa aatttatcac acctagtata gcatactttc
     1501 catcttcaaa aaatttttta aaatctactc atattcctcc atccatttac gaataaatag
     1561 gtatgaagac aattatccta tttttccttg gagcttctat cggctccttc ttgggattgg
     1621 tcatcgaccg tttccctgaa cagtccatta tcgccccctc tagtcactgc aatgcctgca
     1681 aacgacggct caaggcatgg gacttaattc cagtcctatc ccagctttcg acaaaatcca
     1741 aatgccgtta ctgcaaggcg aagatacctt attggtatct gggactagaa ttcttagctg
     1801 gtctagttgt cctgctctgc cattttcaag tcctaaacct aaccgaaacc attctcatct
     1861 tggcaggact agttttgacc atttacgaca tcaaacatca ggaatatcct tttgctgtct
     1921 ggctcatttt tacttttatt gctctgatac tctcccagct caactggctc ttctgtggct
     1981 ttttactctt ggcctatctg actgaaaaat ggcaaatcaa tattggttct ggtgactttc
     2041 tctatctggc aagtttggcc ttaatatgtg gatttacaga actcctctgg attatccaga
     2101 ttagttccct cctagggctt cttgtcttcg ccattttcaa acccaagtct attccctacg
     2161 taccactcct ttttctttca agtattccta tcattctgtg catctaaatc gagtttttct
     2221 cgattttttt gttataatag aaggcatatt agagttcaac tccatcaaaa taattggagt
     2281 gccttcttga tcggaaggca aaaggagaaa cgttatgaca ctatttaagg atgacagttt
     2341 aaccctgcat accgaccttt atcaaatcaa catgatgcag gtctattttg accaaaacat
     2401 ccacaataaa cacgctgtat tcgaagtcta tttccgttca caacctttta aaaatggcta
     2461 cgctgtattt gccggtttgg aacgcgttgt aaactacctc aacaatctca gctttagcca
     2521 gacagacatt gactatctga cagaactagg ttatccgcag gatttccttg attacttgtc
     2581 ccaattggaa atgactctca ctgtccgttc ggctaaggag ggcgacctag tctacgctaa
     2641 cgaacctatt cttcaaatcg aaggaccatt ggcacaatgc caactaatcg aaactgccct
     2701 cttaaacatc gtcaactacc aaaccctgat tgccacaaaa gctcgccgta tcaaggctgt
     2761 cattgaagat gagcccttga tggaatttgg cactcgccgt gcccaagaaa tggatgctgc
     2821 tatctggggg actcgagcag cctttattgg tggagcatct gcgacttcca atgttcgagc
     2881 tggcaaaatc ttcggcatcc ctgtcgctgg tacccatgcc cacgccttgg ttcaaacata
     2941 cggagatgat tacaaggctt tcaaagccta cgctgaaacc catcataact gtgtcttcct
     3001 cgttgatacc tatgatacgc ttcgtattgg tgttccagca gccatcaagg ttgccaaaga
     3061 aatgggagat aagattaact tcaaaggggt tcggattgat tcaggcgata tggcctatat
     3121 ttccaagaaa gttcgtcagc aattagatga tgcaggtttt acagatgcga agatttacgc
     3181 atctaatgac ctggatgaaa ataccatcct caacctcaaa atgcaaaagg caaaaattga
     3241 tgtctgggga gttggtacca aactcatcac cgcctacgac caaccagctc tgggcgctgt
     3301 ctacaagatt gtatccatcg aagatgaaga tggaaatatg cgtgacacca tcaagctgtc
     3361 ttctaatgcg gaaaaagtct ccactcctgg taagaaacag gtttggcgca tcacctccaa
     3421 agaaaaagga aaatcagaag gggactacat cacctttgcg gacactgacg tcacacagat
     3481 ggataatatc tatatgttcc atccaactta cacctatatc aataaaacca tcgagaactt
     3541 tgatgcccgt cctctcctag ttcctatttt cgaaaaagga aaacaggtct atgaacttcc
     3601 aagcctagcg gaaattcaaa cctatgccaa tcaagaattt gataaactct gggatgaata
     3661 caaacgcgtg ctcaacccac agctctatcc agtcgacttg gcacaagatg tttgggataa
     3721 taagatgaac ctcatcaatc gtattcgcaa acaaactcag accaaatcca tctaagaaag
     3781 gtatgttatg accctacagg aaacaatcat caaagaatta ggcgtcaagc caagcattga
     3841 ccccaaagaa gaaatccgtc gctccatcga ctttctcaaa gactatctga aaaaacaccc
     3901 atttttgaaa acctacgtct tggggatttc tggtggacaa gattcgacct tggctggtcg
     3961 tttggcccag ctgactatgg aagaaatgcg ggcggaaaca ggtgacgatt cttatcaatt
     4021 tatcgctatc cgcctgcctt acggtgtgca agctgatgag tctgacgccc aagcagccct
     4081 agcctttatt caaccagatg tcagcttaac catcaacatc aaggaatcaa cagatgccat
     4141 ggtggctgca gtcaatgcaa atgggcttgc tatgtctgat tttaataagg gaaatgctaa
     4201 agcccgcatg cgcatgattg cccaatacgc tatcgctgga gaacgcaagg gggctgttat
     4261 tggaaccgat catgccgctg aaaatatcac tggtttcttt accaagcacg gtgatggagg
     4321 tgcagatatc ctgcccctct tccgccttaa caagcgtcaa ggtaagcaac ttctagctgc
     4381 tttgggagca gatgaaaaac tctacctcaa agttcctact gcagacttgg aagaagacaa
     4441 accaggcctt gctgacgaag tcgcacttgg tgttacctat aaccaaatcg acgactatct
     4501 cgaaggtaaa accatcgacc ctcaatccca aaccattatc gaaggatggt ggaacaaaac
     4561 agcccataaa cgccacctgc caattactat ttttgatgat ttttggaaat aatactcctc
     4621 gaaaatcaaa atcagacgtt attgacttga tttgatgact gaaaagctcc tgtggaggtt
     4681 ttcagcctgt tcccttgaaa tataaaagga acagagttct aacatcaact tgaaggcgca
     4741 aacaatgttc gctatatttc caaccttcaa ctatctccca gatagttgaa gctaggctac
     4801 ttttgatttt catcgaataa taaaaaatgg agccctgaca gttgaaccgt caggggtttt
     4861 gttttttatt ataacttcaa accataatga aaaaagccca cgcaggaaaa aatatcctgc
     4921 gtgggcgtca atggacacgg tgccacctca ctatggactc gactagaaaa gtccataact
     4981 ttttctcctc taacaaggag gcgcacgata aggggtgcgg accgaactct cattgtttcg
     5041 aattcatcga accaaactca ctttactaaa ccagacaatc cataaaatgg atacgaatgc
     5101 ttgtttggca tcagcctatc tcataaacct ctatcatcta ttcacaccaa ccatagactc
     5161 tctaaagaca aagtgtttat tacattttct gattacttgt aattatacgt tacaaaatca
     5221 aaatggtcaa gtgattttta gaaattttct aaacaatttg aaaattcagt ttccattgcc
     5281 tgaatccatt aaaaactaac aaaaagccac tctgcttaag tgactttcta aaaaactgta
     5341 ttcacgagcg aagcactcat atcaaagata gctttagcta gtcaaggtca tttttaaggg
     5401 aatactgact gtatttcaaa aaaataacaa tgtgtagcat aaacaatagc cttagatgga
     5461 cattctgaac ctgtgacggt aggttctaag actcctgttt ggaaaggatt atccctctca
     5521 ttatcaatga ataggtaaaa taggctagta ttccccccat tgtattggtc cataaatcat
     5581 cgatttcaaa gacgcgttgg gcatcaatca gcaaatctag tagtaattgt gtgcattcaa
     5641 taaaaagact gatacaaaaa ctataacgaa tcaccgctcg aaggctccgc cattcttccc
     5701 taagaaacaa aagaagaaaa atcagaggga aaagtagaaa aatattagtg atgttttgca
     5761 agaaaatcca ccctaggtcc gccaaactat ctaccttgtc cccgttgacg atgctgttga
     5821 aaggagttgg taaaagatag agacggccaa tttgaataat tcccggggtc gagaaatcct
     5881 tatactgtgg gtaccaagtc tgaggtagaa aacaaagtaa aacaatagcc agagcatagg
     5941 ctccagctaa ggtcttacaa atctgtcttc ctagcatggt caagtttcca tctgcttgga
     6001 agaattttct catgctcgaa tacctgcctt tgcttttgcg gctgcaatac gagcacgagc
     6061 tttttcacgg tctttcttga gcgtattgga acgcaattga ccacaggcag catcaatatc
     6121 tgtaccatgt tcctgacgaa cgacacagtt aacaccattt ttcttcaaaa catcaaagaa
     6181 agcaagagta cgttccttgg ttgaacgact gtactggtca tgttcactaa ctgggttata
     6241 aggaatcaag ttgatataag acaatttacg aatgttcttg gtcaaatcag ctagttcctg
     6301 cgcctgttcc actccatcgt tgacctcatt gagcataata tactcaaaag tcacacggcg
     6361 gttggtcact ttgatatagt cttcgattgc ttcaaaaaga acttctatag ggaatttgcg
     6421 gttaatgcgc ataatgctcg aacgaaggtc attatttggc gcatgtagag atactgccaa
     6481 gttaacttgg acaccttcac atgcaaactc acgaattttc ggtgctaaac ctgacgtaga
     6541 aacagtgatg tgacgggcac caattgccaa gcccttgtcg tcattaacca ctcgcaagaa
     6601 ggtcataaca ttatcgtagt tatcaagcgg ctcaccaatc cccatcacaa cgatatggct
     6661 aacacgctca ttctgattgc gctcatccaa gtacttttgt accaacataa tctgagccac
     6721 aatctcaccg ctagtcaagt cacgttgctt aggaatcaat ccagatgcac agaatgtaca
     6781 gccgatatta cagccaactt gtgtcgttac acagactgaa aggccatagt gctggtgcat
     6841 gagaactgtt tcaatcaaca tgccatctgg cagttcaaaa aggtatttga tagtaccgtc
     6901 cttagactcc tgtacaatcc gttgtttgag cggattaact acaaaatgtt cttccaactt
     6961 ttcaatcaaa gattttggca agttggtcat ttccgcaaaa gattggacac gagaacggta
     7021 aagccattcc caaatttgag tggcacggaa cttcttctct ccgttttcta aaatccaatc
     7081 aaccagattg gcttgactaa atgcataaat tgatggtttc atatttctcc ctatttctgg
     7141 cggataacaa agttacgctt cacttcattc atctcacgct tactctgctt acgctcatgt
     7201 ttttgagaac gctccgtttg actcgcctta acaccagcat tcttcatagt aaaatgacgc
     7261 tgttccgact gtttattttg ctgcttccct tttttctgac ggtcattgcg attacttttg
     7321 ttttcttttg gctcaataag ctccgtccgt tgctgatttc tgttccgatt gcgagaacga
     7381 ctccgcttcc gacgcggtct atcctcctcc tcagcaggca agtcttgtgg attcggattg
     7441 gctaagacga aataagcaca accaaaatta caatactccg tcaaatagtc gtccaagcgg
     7501 ctgattttta agtctgattt aacattttta tcgtctgtat agaatcccct taatctcagc
     7561 tgctcattgc cccaatctcc cacgatataa tcaaatttga gcatgagttg tgaaaaacgt
     7621 tgaccaaaaa tcgtctggtc aaaagcattc ttgtaatctt ccaaaaggtc caattcaata
     7681 ttttcagata caaccttatc tccgacacga gcaaaactcg gaccagggta tttattatag
     7741 ttatacattt caggtgaaat ttcttttttc atttgtcctc ctatcttata aaatcttcat
     7801 acgtaaaaat catttgatta ataatctatc tggtc
//
LOCUS       DBNFEG010000106         7789 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.106, whole genome shotgun sequence.
ACCESSION   DBNFEG010000106 DBNFEG010000000
VERSION     DBNFEG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.106"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            230..451
                     /gene="relB"
                     /locus_tag="AC1PNG_001477"
     CDS             230..451
                     /gene="relB"
                     /locus_tag="AC1PNG_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HIP6701976.1"
                     /translation="MSTVTIRLNQEEEVFFKSYAQLTGQSLSSLFKKALERDIEDEYD
                     LKIYHQAYDEYKADPETISHADFKKELGL"
     gene            453..713
                     /locus_tag="AC1PNG_001478"
     CDS             453..713
                     /locus_tag="AC1PNG_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE family
                     toxin"
                     /protein_id="HIP6701977.1"
                     /translation="MYHLEYSKKAQKQIKKLDRQIQRLLFAWIDKHLEGTDNPRANGK
                     GLTGNHANEWRYRIGDYRLICDIQDDKLVILALEFGHRKDVY"
     gene            880..1521
                     /locus_tag="AC1PNG_001479"
     CDS             880..1521
                     /locus_tag="AC1PNG_001479"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIP6701978.1"
                     /translation="MIEWIFFDLGSTLLDEEAAYGYYIDKCVKKLESLDIEVSSDSYK
                     KKMVEYAHKSLDPIRATWHYFAPTEPRPLWTNEGVSLYPETIDALEKLSQNYRLGIIA
                     NQSSSIRELLKEWGIESYFQLIILSEEVGLSKPNTAIFTLALQKTNIPADRVVYVGDR
                     YDNDILPAKSLGMWTVRILTGFGKHASENEKLKSDWVIPSLQEITNIFEQTKN"
     gene            complement(1554..1739)
                     /locus_tag="AC1PNG_001480"
     CDS             complement(1554..1739)
                     /locus_tag="AC1PNG_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701979.1"
                     /translation="MNPVDLFNQVKELIANKDFDGAKQFIEENKDQFGEYLEQAKGLL
                     SGSEGVNGLLDKVKGLF"
     gene            complement(1803..2807)
                     /locus_tag="AC1PNG_001481"
     CDS             complement(1803..2807)
                     /locus_tag="AC1PNG_001481"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HIP6701980.1"
                     /translation="MAIYFGTYTKRESKGIYKANFDSETGQLSNLELVTEEPNPTYLA
                     FDKAGHLYSVGAENGLGGIAAFNAEYTLLNHVVSEGAPLCYVAVDEARDLVYGSNFHK
                     GQLLVYKRLADGSLELADVAQHEGSGPHENQASAHVHFSDLTPDKFLVTCDLGCDQVV
                     TYKVSDQGKVEKIATYQAAPGSGPRHIVFHPVAKIAYLICELNSTIEVLIYDGWGQFE
                     HLQTVSTLPEDHTGFNGTAAIRITKDGKFIYGSNRGNDSIAVYKTLADASLELVEIVP
                     TNGKTPRDFTLSPDEKHLIVVHQDSDNATVFERDAENGCLTELSHDFYVPEAVCVTFL
                     "
     gene            2943..4109
                     /locus_tag="AC1PNG_001482"
     CDS             2943..4109
                     /locus_tag="AC1PNG_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIP6701981.1"
                     /translation="MEDKHQKFSLTWFFRLFLNSQAVTFLLVTLLTFLTIFIFSKISF
                     LFRPIGSFLEIVLLPMILTGLLYYLLNPMVDWMEKHKISRTVGISILFVLISLLIIWG
                     LAVAIPSIQEQVTSFAQNLPSNIQKIEGQVTGLLQDQRFEQFRPTALEMLNKVNDQIV
                     AYAQKFSSSAVNWASNLISTASQIIVAILIMPFILFYLLRDGQYLNKHITQYLPTKWR
                     EPIGTVLSDVNGQLSNYVRGQVTVAIIVALMFSVMFSIIGLSYPITLGVMAGFLNLIP
                     YLGSFLAMIPAVILGLIAGPIMLIKVLVVFMIEQTIEGRFVTPLIIGSSLSIHPITIL
                     FVLLTAGQMYGVLGVLLGIPIYASIKVLVKAAFEWYKAHSSLYEDEENEVVNEQ"
     gene            4099..5328
                     /locus_tag="AC1PNG_001483"
     CDS             4099..5328
                     /locus_tag="AC1PNG_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701982.1"
                     /translation="MNNSEKMLVCLDQQDLDKADKYFKRALVQDDSETLLSLAAYLEG
                     IGFFPQARQIYEQVKEEFPEVLINLAQIAFEDGLVEEAFRYLEEISEDSSIYVEALLV
                     KADLYQAEGLSDVACEKLLEASYLSDEPIILFGLAELDMELELFNEAISYYAQLDNRE
                     IYELMGVSTYQRIGIAYASLGKFEAAIEFLEKAVELEYDDQTVFELAALLFEREEYQK
                     ANLYFKQLDTINPDFEGYEYAYAQSLHAEHKIDDALVMTQKGLAKNDFDANLLLQASQ
                     YAYELHDEAGAEDYLLRAKEVADDSNELALRLSNLYLEQERFEEVVALFDEEVDNVLA
                     RWNKAKAYQALERDDEAIALYDELAGELAENPEFLADYVAVLRQLGRLDEAKEQASRY
                     IQLVPDDLAMQEFLNEE"
     gene            5332..6048
                     /gene="budA"
                     /locus_tag="AC1PNG_001484"
     CDS             5332..6048
                     /gene="budA"
                     /locus_tag="AC1PNG_001484"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019322637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HIP6701983.1"
                     /translation="MQVNRLFQYNTLGALMAGLYGGSLTVGELLEHGDLGLGTLDSID
                     GELIVLDGKAYQAKGAGEKPEVVEVPADMKVPYAAVIFHEAEVIFKQRFEMTSDELHQ
                     RIESYYDGENLFRSIKIKGTFSHMHVRMIPKSAFDVRFAEVASRQPEYTAENISGTIV
                     GIWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDYVISEGMVEVGPVDQLDQRFPVQDR
                     QYLFAKFNAKEVREDIDKAE"
     gene            complement(6074..7732)
                     /locus_tag="AC1PNG_001485"
     CDS             complement(6074..7732)
                     /locus_tag="AC1PNG_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990112 - RQC complex [Evidence IEA];
                     GO_function: GO:0001968 - fibronectin binding [Evidence
                     IEA];
                     GO_process: GO:0072344 - rescue of stalled ribosome
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein"
                     /protein_id="HIP6701984.1"
                     /translation="MSFDGFFLHHMTAELKANLEGGRIQKINQPFEQEIILNIRSNRQ
                     SHKLLLSAHSVFGRVQLTQSDFTNPKVPNTFTMILRKYLQGAIIEEIRQLDNDRILEF
                     SVSNKDEIGDHTQATLIVEIMGKHSNIILVDKSEQKIIEAIKHVGFSQNSYRTILPGS
                     TYIRPPETHSLNPYTVSDEKLFEILSTQELSPKNLQQVFQGLGRDTASELASHLQTDR
                     LKNFRAFFDQATQPSLTAKSYAALPFANSPENQPHFESLSSLLDFYYQDKAERDRVAQ
                     QANELIKRVASELEKNRKKLVKQEQELADTETAELVRQKGELLTTYLHQVPNDQASVT
                     LDNYYTGQELEIELDVALTPSQNAQRYFKKYQKLKEAVKHLTNLIEETKATIVYLESV
                     DTMLGQASLAEIDEIREELIETGYLKRRHREKIHKRQKPERYLATDGKTIILVGKNNL
                     QNDELTFKMAKKGELWFHTKDIPGSHVVITDNLDPSDEVKTDAAELAAYFSKARHSNL
                     VQVDMIEAKKLHKPTGGKPGFVTYRGQKTLRVTPTEDKIKSMKI"
BASE COUNT         2176 a         1429 c         1776 g         2408 t
ORIGIN      
        1 gtttttcgta gtacagtgta ctaatttatc cccgagctag tatagtgagg gatatagtaa
       61 gaacaagtct gaaaatattc ggacttgttc tttgacttta tatgatttaa gggctgtaaa
      121 gccttgatat aaggcacttt ggggcatcat cccaaggtac ttttttgata gattgacaga
      181 tactttaaaa atgtgttaca atgtaacaca aagaagcgag gtgaatatca tgagtacagt
      241 gacaatcaga ttaaatcaag aagaagaagt attctttaaa agctatgcac aattaacagg
      301 tcaaagtctt tctagccttt ttaagaaagc tttggagcgt gatattgaag atgagtatga
      361 tttaaaaatc tatcatcaag cttatgatga gtacaaagca gatccagaaa caattagtca
      421 tgctgatttt aagaaagagc taggattata gcatgtatca tttagagtat tctaaaaagg
      481 ctcaaaagca aattaagaaa ttagataggc aaattcagag gttgttgttt gcttggattg
      541 ataaacactt agaagggact gataatccac gcgccaatgg taaaggtttg acaggcaatc
      601 atgctaacga gtggcgttat cgtatcggag attaccgact gatttgcgat attcaagatg
      661 ataagttggt tattttagca cttgagtttg gacatcgtaa agatgtctat tgatgcgtga
      721 aaatctatga caagataggt cctaactatt tttaagaatg aatagaaaac tagactgaaa
      781 ttcctaggga attttctttg ccttgatatt gataaaatga tattctaaaa aggcagatca
      841 acttaagtat tctcattttg agaagagatg gagagtggga tgatagagtg gatatttttt
      901 gatttgggtt cgacgctttt agatgaagag gctgcttatg gttattatat agacaagtgt
      961 gtgaagaagt tagagtctct ggatatagag gtttcatcag attcttataa aaagaaaatg
     1021 gtggagtatg ctcataagtc gctagatcca attcgtgcga cctggcacta ttttgcaccg
     1081 accgagccac gtcctttatg gacaaatgag ggtgtgagcc tgtatcctga aacaatagat
     1141 gctctagaga agttatcaca aaactatcga ttggggatta ttgccaatca gtctagtagt
     1201 attagagaac tgcttaaaga gtggggaatt gagtcttatt ttcaactcat catcctctct
     1261 gaagaggttg gtttatctaa accaaataca gctattttca cacttgcctt gcaaaaaaca
     1321 aacatccctg cagatagagt tgtctatgtc ggggataggt atgataatga tattctgcct
     1381 gctaaatctt tgggaatgtg gactgtacgg atactgacag gttttgggaa gcatgccagt
     1441 gaaaatgaaa aattgaagag cgattgggta attccttcat tgcaagagat aacaaatatt
     1501 tttgaacaaa caaaaaacta gactgaatat tctcagccta gtttttacat gtattagaac
     1561 aagcctttaa ctttatctaa aagaccgtta acaccttcag aacctgataa caagcctttt
     1621 gcctgttcca agtattcacc gaattggtcc ttgttttctt cgataaattg ttttgcacca
     1681 tcaaagtctt tgtttgcaat caattctttt acttggttaa aaagatctac tggattcatg
     1741 ttgaaatcac ctcctatgag actattaaac caaaactgta cggatttcac aattgaaacg
     1801 acttataaaa atgtaacgca aactgcttca ggaacataga aatcgtgtga aagctcagtc
     1861 aaacagccat tttcggcatc tctttcgaat acagttgcat tgtcagaatc ttgatggaca
     1921 acaatcaaat gcttttcatc aggactaagc gtgaaatcgc gaggagtttt tccatttgtt
     1981 gggacgattt caactaattc taagctggca tcagcaagtg ttttgtagac tgcgatagag
     2041 tcgtttccgc ggttggatcc ataaataaat ttaccatcct ttgtgatgcg aatagcggca
     2101 gtaccgttga aaccagtatg gtcttctggg agagtagaga cagtttgtag gtgttcaaat
     2161 tgtccccagc cgtcatagat aagcacctca atagttgaat tgagttcaca gatgagatag
     2221 gcgatttttg cgacaggatg gaagacaatg tgacgtggac cacttcctgg cgcagcctga
     2281 taggttgcta ttttctctac ttttccttgg tcagatacct tataggttac cacttgatca
     2341 catcctaaat cacaggttac taaaaattta tcgggcgtca aatcagaaaa gtgaacgtgg
     2401 gcggaagctt ggttttcgtg cggcccagat ccttcgtgtt gagcgacatc agctaattct
     2461 agcgaaccat ccgccaaacg tttataaaca agtagttgcc ctttgtggaa attggaacca
     2521 taaactaaat ctcttgcttc atctacagcg acatagcaaa gtggtgcgcc ctctgataca
     2581 acatggttga gtagagtata ctcagcatta aaggcagcga tacctcctag accattttca
     2641 gcgccgacgg agtatagatg tccggcctta tcaaaggcaa ggtaggtagg gttaggttct
     2701 tcagtgacca attctagatt gctaagttgt cctgtttcag agtcaaaatt tgctttataa
     2761 attccttttg attcacgttt tgtataggta ccgaaataga tagccatagg attctccttt
     2821 ttggaaatga ttgataaaat tataccatgt ttttctgaga aagtcgtata aattctgtag
     2881 ttgattttta caaaaatcct ttctccatgt tacaatagaa aaaaagacta ggagtgtatt
     2941 agatggagga taagcatcaa aaatttagtc tgacttggtt tttccgcttg tttttaaata
     3001 gccaagcggt gacattttta ttggtaacct tgctgacttt tttgaccata tttattttta
     3061 gtaaaattag ttttttattc agaccaatag gaagcttttt agaaattgta ttgttaccga
     3121 tgattttaac aggtcttttg tattacctat taaatccgat ggttgactgg atggaaaagc
     3181 ataaaatttc tcgtacagtt ggtatttcca ttctctttgt tttaatcagt cttctcatta
     3241 tctggggact ggcggtggca attccgagta ttcaagagca agtgacatct tttgcgcaaa
     3301 atcttccttc aaatatccag aaaatcgaag gacaggtaac tgggctccta caggatcagc
     3361 gctttgaaca atttagacca acagctctgg agatgttaaa taaggtcaat gatcagattg
     3421 ttgcctatgc tcagaagttt tcttcaagcg ctgtgaactg ggcaagtaat ttgatttcaa
     3481 ccgcttcaca gattattgtt gctattttga ttatgccctt tattctcttt tatctgttac
     3541 gggatggtca gtatttgaat aaacacatta cccaatatct accaaccaag tggagagaac
     3601 cgattggaac agtactatca gatgtgaatg ggcaattatc aaactatgtt cgtggtcagg
     3661 tgaccgttgc gattattgtt gccctgatgt tctcagtgat gttctcaatc attggtctta
     3721 gctatccaat aactttgggg gtgatggctg gtttcctcaa tctaattccg tatttgggtt
     3781 ctttcttggc catgattcca gctgttattt taggattgat tgctggtcca atcatgctga
     3841 taaaggtttt ggtagtcttt atgattgagc agactataga aggtcgtttt gtgacaccat
     3901 tgattatcgg aagctcccta agtattcatc cgattaccat tttatttgta ttattgacag
     3961 ctggtcagat gtacggtgtc ctaggagttc ttctgggaat tccaatatat gcctctatta
     4021 aggttttagt gaaagcggca tttgaatggt acaaggcaca cagtagtttg tatgaagatg
     4081 aagaaaatga ggttgtgaat gaacaatagt gaaaaaatgt tggtttgttt ggaccaacag
     4141 gatttagaca aggcagataa gtatttcaag cgcgccttgg tgcaagatga tagcgaaacg
     4201 cttttgtcct tggcagccta cttagaaggc attggttttt tcccacaggc tagacaaatc
     4261 tatgaacagg tcaaagaaga atttcccgaa gtactgatca atcttgccca aatagcattt
     4321 gaagatggct tggttgaaga agctttcaga tatttggagg aaatttcgga agacagttct
     4381 atctatgtgg aagccttgtt ggtcaaggcg gacctgtatc aggcagaagg cttgtcggat
     4441 gtggcgtgtg aaaaattgtt agaagcaagt tacttatcag atgaacccat catcctcttt
     4501 ggcttagcag aattggatat ggaactggag ctttttaatg aggctatttc ctactatgcc
     4561 cagctggata atcgtgaaat ctacgaatta atgggagttt ctacctatca gcgaattggt
     4621 attgcctatg caagtttggg gaaatttgaa gcggcgattg aatttttgga aaaagctgtt
     4681 gagttggaat acgatgatca gacggtcttt gaattggctg ctctgctatt tgaacgagaa
     4741 gaataccaaa aggccaatct ctatttcaag cagttggata ccatcaatcc tgattttgag
     4801 ggctatgaat atgcctatgc tcagtctctt catgccgagc ataagattga tgatgccttg
     4861 gttatgactc aaaaaggcct ggctaaaaat gactttgatg ccaatctctt gcttcaggct
     4921 tcacagtatg cctatgaatt gcacgacgag gcgggggcag aagattatct tctacgggca
     4981 aaagaagtgg cagatgatag taacgaacta gctctgcgct tgtccaattt gtatcttgag
     5041 caggaacgat ttgaagaggt tgtggcttta tttgatgaag aggtagataa cgttctagct
     5101 cgctggaata aggccaaagc ctatcaagct ctagaaagag atgatgaagc tattgcgctt
     5161 tacgatgaat tggctggaga attggctgag aatcctgaat ttttggctga ttacgtagcg
     5221 gttttacgtc aacttggtcg tttggatgag gcaaaagagc aagctagtcg ttatatccaa
     5281 ttggtaccag acgatttagc catgcaagaa tttttgaatg aagagtaggt tatgcaagta
     5341 aatcgattat ttcaatacaa taccttaggt gccttaatgg ctggactgta tggcggctcc
     5401 ttgacagttg gcgaattgtt ggaacatggt gatttgggtt tgggaaccct ggattccatt
     5461 gatggcgagc tgattgtctt ggatggaaag gcttatcagg ccaagggtgc aggggagaaa
     5521 ccagaagtgg ttgaagttcc agctgatatg aaagttccct atgcggcggt tattttccat
     5581 gaagcagaag tcattttcaa acaacgcttt gaaatgacta gcgatgaatt gcaccagcgg
     5641 attgagtcat actatgacgg ggaaaatctt tttcgctcca tcaaaatcaa agggacgttt
     5701 tcgcatatgc acgtgcgcat gattccaaaa tcggcttttg atgttcgttt tgcggaggtg
     5761 gcaagtcgcc agcctgagta taccgctgag aatatttcag gtaccattgt tggtatttgg
     5821 acaccagaaa tttttcacgg ggtcagtgtg gctggctatc atttgcattt tatttcggat
     5881 gatttgacat ttggcgggca tgtcatggac tatgtgattt cagaaggaat ggttgaagtg
     5941 ggaccagtcg atcaactaga ccaacgtttt cctgttcaag accgccagta tctttttgct
     6001 aagttcaacg ctaaggaagt tagagaagat attgataagg cggagtaggt ttcaatacag
     6061 aaaaatccat gaattagatt ttcatggatt ttattttatc ttcagttggg gtgacacgga
     6121 gggttttctg acctcggtag gtcacaaagc ctggtttgcc acctgttggc ttatggagtt
     6181 ttttggcttc aatcatatcg acttggacca aattggaatg tctggcctta gagaagtagg
     6241 ctgctaattc agctgcgtct gtcttgacct cgtcacttgg gtccaagttg tctgtgatga
     6301 ccacatgact acctggaatg tctttggtat ggaaccagag ttcgcctttc ttggccattt
     6361 tgaaggtcaa ctcatcattt tgcaggttat ttttcccgac cagaataata gtcttcccgt
     6421 ccgtcgccaa gtatcgctca ggtttctgac gcttatggat tttctcacgg tggcggcgtt
     6481 tgaggtagcc tgtttcaatc aattcctcac ggatttcatc gatttctgcc agactagcct
     6541 gtcccagcat ggtgtcaacg gactccagat agacaatggt tgccttggtc tcctcaatca
     6601 agttggtcag gtgcttgacc gcctccttga gtttctggta ctttttgaaa taacgttggg
     6661 cattctggct aggagtaaga gccacgtcca actcaatctc cagttcctgg cccgtatagt
     6721 agttgtctaa ggtcacacta gcctggtcat ttggtacttg atgcagatag gtggtcaaga
     6781 gctcgccctt ttgtcgcacc aactccgccg tctccgtatc cgccaattcc tgctcctgct
     6841 tgaccagttt cttgcgattt ttctccaact cactggctac ccgcttgatc agctcattgg
     6901 cctgctgggc cacccgatcc cgctccgcct tgtcctgata gtagaagtcc agcagactgc
     6961 tcaggctctc aaagtgtggc tggttttcag ggctattggc aaatggcaga gcagcatagg
     7021 atttagctgt caggctaggc tgagttgcct ggtcaaaaaa ggcacggaag ttcttgaggc
     7081 ggtccgtctg taagtgactg gccagttcag aagctgtatc ccgccccaaa ccttgaaaga
     7141 cctgttggag attttttgga ctcagttcct gagtcgataa gatttcaaac aatttctcat
     7201 cagacaccgt ataaggattg agagaatgtg tctccggtgg acgaatatag gtggagcctg
     7261 gcaagatagt tcggtaggag ttttgcgaga aaccaacgtg cttgatagcc tcaataatct
     7321 tctgttcaga cttatctacc aagatgatat tgctgtgctt gcccatgatt tccacaatca
     7381 aggttgcttg ggtatggtcg ccaatctcgt ccttgttgga caccgaaaat tctaagatgc
     7441 ggtcattgtc caactgacga atctcctcga taatggctcc ctgcaagtat ttccgcagaa
     7501 tcatggtaaa ggtattgggc actttgggat tggtaaagtc cgactgggtc agctgaaccc
     7561 gaccgaaaac cgaatgggcg gacaagagca atttatggct ctgacggttg ctacggatat
     7621 tgaggataat ctcctgttca aagggctggt tgattttctg aatccgccca ccttctaaat
     7681 tggcctttaa ctcagccgtc atgtgatgta aaaaaaatcc gtcaaaagac atggttttcc
     7741 tctttctaat agctataact cagtctagta tatcataaat cagtgactt
//
LOCUS       DBNFEG010000107         7753 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.107, whole genome shotgun sequence.
ACCESSION   DBNFEG010000107 DBNFEG010000000
VERSION     DBNFEG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.107"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(27..479)
                     /locus_tag="AC1PNG_001486"
     CDS             complement(27..479)
                     /locus_tag="AC1PNG_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1694 domain-containing protein"
                     /protein_id="HIP6701985.1"
                     /translation="MNDLNTTILQKASGETRLNPDEQRLYMGTYRERVVLIVSFDDLS
                     SEVVGNKFDTICQKLANRYSPLFLKLSPALSDKQQISLLKIAQNFGITTAIIDEKIGQ
                     SPYALVFHTNHAVDNEEVSLESIFPNLISKPEEKKEDAKPSFWKKLFG"
     gene            629..1936
                     /gene="eno"
                     /locus_tag="AC1PNG_001487"
     CDS             629..1936
                     /gene="eno"
                     /locus_tag="AC1PNG_001487"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-displayed alpha-enolase"
                     /protein_id="HIP6701986.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYLGLGTQKAVDNVNNVIADAIIGFDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYTYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPSFKEGLRWGAEVFHALKKILKARGLVTAVGDEGGFAPKFEGTEDG
                     VETIIEAIEAAGYEAGENGIMIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQI
                     DYLEELVNKYPIITIEDGMDENDWDGWKALTERLGKRVQLVGDDFFVTNTDYLARGIK
                     EGAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAVYKGLNSFYNLKK"
     gene            complement(2096..3259)
                     /locus_tag="AC1PNG_001488"
     CDS             complement(2096..3259)
                     /locus_tag="AC1PNG_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIP6701987.1"
                     /translation="MRIKDKIKKNGQKVYYASVYLGVDQLTGKKARTTVTATTQKGVR
                     VKARDAINAFAANGYTVKDKPTITTYKELVKVWWDSYKNTVKPNTRQSMDGLVRVHLL
                     PVFGDYKLSKLTTPIIQQQVNKWADKANTGQKGAFTNYSLLHNMNKRILKYGVSMQVI
                     TYNPANDVIVPRKQQKEKAGVKYLDNKELKQFLDYLDTLDQSNYENLFDVVLYKTLLA
                     TGCRIGEALALEWSDIDLDNGIVSINKTLNRFQEINSPKSSAGYRDIPIDNATLLMLK
                     QYKNRQQVQSWQLGRSETVVFSVFTEKYAYACNLRQRLNKHFKAAGVTNVSFHGFRHT
                     HTTMMLYAQVSPKDVQYRLGHSNLMMTENVYWHTNQENAKKAVSNYETAINSL"
     gene            complement(3343..3888)
                     /locus_tag="AC1PNG_001489"
     CDS             complement(3343..3888)
                     /locus_tag="AC1PNG_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6701988.1"
                     /translation="MNRLKDLRTKHKLTQQQTADIAGVTRRGYQKWESGESSIKADKA
                     QILADYFGVPVAYLLGYHEETISFTFPNDDEGFYKETQKLIDIADNIDPNSEIYSQVQ
                     QVKDMAILARSSREKIKARYGQKVYAGLNDKETDFEFYQYLGFLLNKEEYLKSDLLVY
                     LSLLDSSDKEIVFKMITEFKK"
     gene            4046..4234
                     /locus_tag="AC1PNG_001490"
     CDS             4046..4234
                     /locus_tag="AC1PNG_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HIP6701989.1"
                     /translation="MIITETQAKAIRRKIADKQLKQYEFAKEIGVASRTVPKIVAGNY
                     KAPKRIYAAVMEWLAKDY"
     gene            4344..4688
                     /locus_tag="AC1PNG_001491"
     CDS             4344..4688
                     /locus_tag="AC1PNG_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701990.1"
                     /translation="MKTIALIKYIYYKFTKNKGENQKMALSTRNIKQQGNQIAELLSR
                     IEIIQQLGNALLLADNAGADSATLHYQMKQAFSVIFEMTEQLYKDLDLIACKLINCDD
                     DKELEVIRQHER"
     gene            4678..4881
                     /locus_tag="AC1PNG_001492"
     CDS             4678..4881
                     /locus_tag="AC1PNG_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701991.1"
                     /translation="MNDKILSNYELLCTELENVISTLEIASGDIEPGKATALVTVATN
                     GLKHLVSEHTELSDKFFKEYGNE"
     gene            4874..5077
                     /locus_tag="AC1PNG_001493"
     CDS             4874..5077
                     /locus_tag="AC1PNG_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701992.1"
                     /translation="MNELIPTLTESVILIIVSLAILILLWRYESYIELDISPQTDKVE
                     ENTTAHVQERYGAYVWLAGKKFN"
     gene            5088..5354
                     /locus_tag="AC1PNG_001494"
     CDS             5088..5354
                     /locus_tag="AC1PNG_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701993.1"
                     /translation="MSTFSVEFEQGLLDRVDKLAQQKLELEKKLQKKTGLITAKELKD
                     DLDISGTTLKNWMDIGLVSYQSPFESSKKLYFKVSDVINFLTVR"
     gene            5365..5523
                     /locus_tag="AC1PNG_001495"
     CDS             5365..5523
                     /locus_tag="AC1PNG_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701994.1"
                     /translation="MKVFIIDGDAWRGKAHYSPSLDVVFISDKVDKHARDELIERVTK
                     SSNRTIWR"
     gene            5528..5743
                     /locus_tag="AC1PNG_001496"
     CDS             5528..5743
                     /locus_tag="AC1PNG_001496"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6701995.1"
                     /translation="MAKEHYEVTSVFPAGWIVNGKKLTEPIEMSAEELRKSEVYPPEN
                     SSVYDIFKKINEERWEETRKDGNKAND"
     gene            5721..6551
                     /locus_tag="AC1PNG_001497"
     CDS             5721..6551
                     /locus_tag="AC1PNG_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HIP6701996.1"
                     /translation="MATRRMISKEVVMTDNFLDLPPTTKVLYFFLNLEADDDGFVGNP
                     KTVMRLTETTKDDMKLLIDSEYVLLFDTGVVVITDWTEHNAIRKDRKKDTRFTKEMQQ
                     IILVDGGKYFWLSDSQPNDNQSATINTPNGCVGEDRRGKDSIGKERVVEVDKEQNQSP
                     TPPPFNQDFVNLYKSFEAETGKALSPIQMQELQYMLEDFSADVIHEALREAVGQGKAN
                     FAYIQAILKRWKQDNLLTVELIRNNKAAREVKKQQNNSKPEPQTREEWLANWSEENPF
                     "
     gene            6567..7412
                     /locus_tag="AC1PNG_001498"
     CDS             6567..7412
                     /locus_tag="AC1PNG_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIP6701997.1"
                     /translation="MLTQAEFIANTKTLEETCPIHGIPLMQLDRVVKIAEEDKPRKLS
                     PICPECIKEQNEKQVQEEVEKHLNAGIYQKTYNVLMRDSTIPEDLKAASFDNFIIETP
                     EEKQMLDFVKNQTQKYLDGMNGNTLLTGTTGVGKTHLAISLAKELNEAYRAKGEPKSV
                     LFINLTEILREIRESFKFTSKEGYYSRMLKEVDYLILDDLGVKLGNASGQSKSAWEEE
                     FIFDVLSHRNNTIITTNLSNDEIANLYSERVASRVRTGLDGNYFKVFSIKDKRYSINK
                     LKSNM"
BASE COUNT         2474 a         1368 c         1579 g         2332 t
ORIGIN      
        1 ttatactgct ttattgataa ataagtctaa ccaaataatt ttttccagaa ggaaggtttt
       61 gcatcttcct ttttttcttc tggttttgag ataagatttg gaaatatgga ttctaagcta
      121 acttcttcat tatccacagc gtgatttgtg tgaaagacaa gtgcgtaggg agactgtcct
      181 attttttcat cgatgatagc ggtagtaata ccgaagttct gagcaatttt tagtagtgaa
      241 atttgttgct tgtcagacag ggctggcgag agtttgagga atagaggaga gtatctgttt
      301 gctagttttt gacaaatcgt atcgaactta tttccgacga cttcactact caaatcgtca
      361 aaggaaacga tcaggacaac ccgttcgcga taggttccca tgtatagacg ttgttcatct
      421 ggattcaatc gtgtttcgcc agaggctttc tgtagtatgg tagtatttaa atcgttcatg
      481 gtttcagtat aacatactca gttaactttg aaaaggaagg cgtttacatt atttctttca
      541 gtaaaataac ctgttttttc ttgaaaaatc ccttctttta gggtatgata gctatgtaac
      601 aattttaaac tcaaaggaga gtaatataat gtcaattatt actgatgttt acgctcgcga
      661 agtccttgac tcacgcggta accctacact tgaagttgaa gtttatactg aatcaggtgc
      721 tttcggacgt ggtatggttc cttcaggagc ttctactggt gagcacgaag cagttgagct
      781 tcgcgacggt gacaaatctc gttaccttgg tcttggtact caaaaagctg ttgacaacgt
      841 gaacaacgtg attgctgacg ctatcatcgg ttttgacgtt cgtgatcaac aagctatcga
      901 ccgcgctatg atcgctcttg acggtactcc taacaaaggt aaattgggtg caaacgcaat
      961 cctcggtgtt tctatcgctg ttgcgcgtgc tgctgctgac taccttgaag tgccacttta
     1021 cacttacctt ggtggattca acactaaagt attgccaact ccaatgatga acatcatcaa
     1081 cggtggttct cactcagacg ctccaatcgc tttccaagaa ttcatgatct tgccagttgg
     1141 cgctccttca ttcaaagaag gtcttcgttg gggtgctgaa gtattccacg ctttgaagaa
     1201 aatcttgaaa gctcgtggtt tggtaacagc tgttggtgac gaaggtggtt tcgctcctaa
     1261 gttcgaagga actgaagacg gtgttgaaac aatcatcgaa gctatcgaag ctgctggtta
     1321 cgaagctggc gaaaacggca tcatgatcgg tttcgactgt gcgtcatctg aattctacga
     1381 taaagagcgt aaagtttacg actacactaa attcgaaggt gaaggcgctg ctgttcgtac
     1441 ttctgcagaa caaatcgact accttgaaga attggttaac aaatacccaa tcatcactat
     1501 cgaagatggt atggatgaaa acgactggga tggctggaaa gctcttactg agcgtcttgg
     1561 caaacgcgtt caattggttg gtgacgactt cttcgtaaca aacactgact accttgcacg
     1621 tggtatcaaa gaaggtgctg ctaactcaat ccttatcaaa gttaaccaaa tcggtactct
     1681 tactgaaaca tttgaagcta tcgaaatggc taaagaagct ggctacactg ccgttgtatc
     1741 acaccgttca ggtgaaactg aagattcaac aatcgctgac atcgcagttg caactaacgc
     1801 aggccaaatc aagactggtt cattgtcacg tacagaccgt atcgctaaat acaaccaatt
     1861 gcttcgtatc gaagatcaac ttggtgaagt tgcagtctac aaaggcttga actcattcta
     1921 caacttgaaa aaataatctc tttatggggt taagggctgt aaagccttga tattaggcac
     1981 tttggggcat catcccaagg tgctttttta gaatttacta ccctttgtac taccccatga
     2041 ttgaaatgct gtattttttg tatagtaaga gggcagtcac aaagtgtgac cccctttata
     2101 aactattgat tgctgtttca taatttgaga cagctttttt tgcgttttct tggttagtgt
     2161 gccaataaac attttcagtc atcatcaagt tagagtgacc tagcctgtat tgtacatctt
     2221 tggggctaac ttgagcgtag agcatcatag tagtatgtgt gtgacggaaa ccatgaaatg
     2281 atacattagt cacgccagca gccttaaaat gcttatttag gcgttgtctc aaattacaag
     2341 cataggcata cttttctgta aatacagaga atacaaccgt ttcagaccgc cctagttgcc
     2401 atgattgtac ttgctggcgg ttcttgtatt gtttgagcat aagtaatgtg gcattgtcta
     2461 ttggtatatc acgataacca gcgcttgatt taggagagtt tatctcttga aatcggttga
     2521 gtgtcttgtt gatgctgaca ataccgttat ctaggtcaat atcagaccat tcaagagcta
     2581 gtgcctcccc gatacggcaa ccagtagcta acaaagtctt gtataggaca acatcaaaca
     2641 agttttcata gtttgattgg tctaaggtat cgagataatc aagaaattgt tttagttctt
     2701 tgttgtccag gtattttaca ccagcttttt ctttttgttg cttacgtggc actataacat
     2761 cattagctgg gttatacgtt atcacttgca tagaaacacc gtatttcaaa atacgcttat
     2821 tcatgttatg gagtaaggag tagttagtaa acgccccttt ttgccctgta ttagccttgt
     2881 cagcccattt gtttacttgt tgttggataa taggcgtagt gagtttagat agcttgtaat
     2941 cgccaaatac aggcaataga tgcactctaa ccaacccatc cattgattgg cgagtatttg
     3001 gcttaactgt attcttgtaa ctatcccacc aaacttttac aagctccttg tacgttgtaa
     3061 ttgtcggctt gtctttaact gtatagccat tagcagcaaa agcattgata gcatcacgcg
     3121 cttttaccct aacccccttt tgggtggttg ctgtaacagt tgtacgggct ttcttgcccg
     3181 ttagttggtc aacgcctaga taaacactag catagtaaac tttttgaccg ttctttttga
     3241 ttttatcttt gattctcatg tatttgtacc tttctttcca tcagcaggca aggcgcgtgg
     3301 ttttgttagg tatttataca cggcttcata gcaagacaat tactattttt taaattcggt
     3361 aatcatttta aaaacaatct ctttatccga tgaatcgagt aatgaaagat acaccagcaa
     3421 atcacttttg agatattctt ctttatttaa tagaaagcct agatactggt aaaattcaaa
     3481 atctgtttct ttatcattta gcccagcata tactttttga ccatatcgag cttttatttt
     3541 ttcacgacta ctccgtgcta gtatagccat gtctttaact tgctgcacct gggaataaat
     3601 ttcactattt gggtcaatat tgtcagctat atcaattagc ttttgagtct ctttatagaa
     3661 accctcgtca tcatttggaa aagtaaaact gatagtttct tcatgatagc cgagtagata
     3721 agcaacgggg acgccgaaat aatcagctaa tatttgtgct ttgtcagctt ttattgagct
     3781 ttcaccactt tcccattttt ggtaacctcg tctagttaca ccagcaatat cagccgtttg
     3841 ttgttgggta agtttgtgct tagtacgtaa atcttttaat ctattcactt tttttcacct
     3901 catagcgatt ataacacaat ttcacgagat aatgctaact ttttttcact tttttatcat
     3961 aaacgcttga caagggaacg aaagttagct aaaatgctat ttgtaatata tttgctaact
     4021 aaagttagct agaaaagagg aaattatgat tattacagag acacaagcca aggctattcg
     4081 tcgaaagatt gctgataagc agctaaagca atatgaattt gctaaagaaa taggtgtggc
     4141 tagtcgtaca gttcctaaaa tagtagctgg taactataaa gcaccaaagc gaatttatgc
     4201 agcagtcatg gaatggttag caaaagatta ttaaggtaca aaaaagccat gtacaggcga
     4261 ccaaaccaac gtacacggct aaggaaaaat aacaaaactc aagcaaaggc atggcgcgtg
     4321 gttttgttag gtatttagta agagtgaaaa ctatcgccct tataaaatat atttactata
     4381 agtttaccaa aaacaaagga gaaaatcaaa aaatggcatt aagcacacga aacattaagc
     4441 aacagggtaa ccagattgct gagttgctct cacgtattga aattatccaa caactaggca
     4501 atgctttatt acttgctgat aatgctggag cagatagcgc tactttacac tatcaaatga
     4561 aacaagcatt tagcgttatt tttgaaatga ctgaacagct atataaagac ttagatttaa
     4621 tcgcttgtaa gttgataaat tgtgatgatg ataaagaatt ggaggttatt agacagcatg
     4681 aacgataaga ttttaagtaa ctatgaattg ctatgtactg agttagaaaa tgttatcagt
     4741 actttagaga tagctagcgg cgatattgaa ccaggcaagg caacagccct tgtaacagta
     4801 gcaaccaatg ggctaaagca tcttgtttca gaacacacag aattatcaga taaatttttc
     4861 aaggagtatg gcaatgaatg aattaatccc aacattgact gaaagtgtta tcttgattat
     4921 tgtttccttg gctattctaa ttttattatg gcgttatgag agctatatag agcttgatat
     4981 tagcccacaa actgacaagg tggaggaaaa caccacagcc cacgtacaag agcgctatgg
     5041 ggcttatgta tggcttgctg gtaagaaatt taactaggag ggggaatatg tcgacatttt
     5101 cggttgaatt tgaacaaggc ttattagata gagttgataa gctagcacag caaaagctag
     5161 agctagagaa gaagttacaa aagaaaacag gtttaatcac agccaaggaa ctcaaagatg
     5221 acctcgatat ttctggcaca actttgaaaa actggatgga tattgggcta gtctcatatc
     5281 aatcaccctt tgaaagtagc aaaaaactat atttcaaggt ttctgatgtg attaattttc
     5341 tcacagtacg ctaggaggta tattttgaaa gtatttatta ttgatggtga tgcgtggaga
     5401 ggtaaggcgc attattcacc tagcttagat gttgtgttta tcagtgacaa agtggataag
     5461 cacgcgcgtg atgaattgat tgagcgtgtg acgaaatcaa gtaatagaac aatctggagg
     5521 taaaattgtg gctaaagaac attacgaagt aacaagtgtc tttccagcag gctggattgt
     5581 taacggaaag aaactaacag aacctattga aatgtcagca gaagaactaa gaaaatccga
     5641 ggtatatccg cctgaaaaca gttcggtata tgacattttc aaaaaaataa acgaagagag
     5701 atgggaggag acgcggaaag atggcaacaa ggcgaatgat tagcaaggaa gttgttatga
     5761 ctgacaactt tcttgattta cctcccacta caaaagtttt gtattttttt ctaaacttag
     5821 aggcagatga tgatggtttt gtcggaaatc ctaagactgt tatgagatta actgaaacaa
     5881 ctaaggatga tatgaaactt ttaattgata gcgagtatgt gctattgttt gatactggag
     5941 tagtagttat aacagattgg acagagcata acgctatcag aaaagacaga aaaaaagata
     6001 ctagatttac aaaagaaatg caacaaataa tattggtaga cggtgggaaa tatttttggt
     6061 tgtcagatag tcaaccaaac gacaaccaat cggcaaccat taatacacca aatgggtgcg
     6121 taggagagga taggagaggt aaggatagta taggtaagga gagagtagta gaagtagata
     6181 aagaacaaaa tcaatcacct actccccctc cttttaatca agactttgta aatctctata
     6241 aatcttttga ggctgaaact ggtaaagcct tatcgccaat acagatgcaa gaattgcagt
     6301 acatgctaga agattttagc gctgatgtta ttcatgaggc attgagagag gcagtaggtc
     6361 aaggtaaggc taattttgct tatattcaag ctattctcaa gcgatggaaa caggataacc
     6421 tattgacggt agagcttatt agaaataata aggcagcgcg tgaggttaag aaacagcaaa
     6481 ataattcaaa accagaacca caaacccgtg aagaatggtt ggcgaactgg tcagaagaaa
     6541 acccatttta gaaaaaggag ttacacatgc taacacaagc tgaatttatc gcaaacacaa
     6601 agacactaga agagacttgc cccattcatg gcatcccgtt aatgcaactt gatagagttg
     6661 ttaaaattgc tgaagaagat aaaccacgga agctttctcc gatttgtcca gaatgtataa
     6721 aagagcaaaa tgaaaagcaa gtgcaggaag aggtagaaaa acacttgaac gcaggtattt
     6781 atcaaaaaac ctataacgtg cttatgcgtg acagtaccat cccagaagac ttaaaagcgg
     6841 catcttttga taatttcatt attgagacac cagaggaaaa gcagatgtta gattttgtaa
     6901 aaaatcaaac tcaaaaatat cttgatggca tgaatggaaa tactttgcta acaggtacta
     6961 caggggttgg aaaaactcat ctagctattt ctcttgccaa agagctaaac gaggcgtata
     7021 gagccaaagg agagcctaaa agtgtattgt ttattaacct tactgagata ctaagagaaa
     7081 tccgagagag ttttaagttt actagcaaag agggttatta ttcacggatg ctaaaagagg
     7141 ttgattatct gattttggat gatttagggg tcaaacttgg caacgcctcg ggtcaatcca
     7201 agtcagcatg ggaggaagag tttatttttg atgtacttag tcatcggaat aatactatca
     7261 tcacaactaa cctaagtaat gatgagatag caaaccttta tagcgaacgt gttgcaagtc
     7321 gtgtgcgtac aggcttagat ggtaactatt tcaaagtatt tagtatcaaa gataagcgtt
     7381 actcaatcaa taagctaaag agtaatatgt gataatgaat taacttacaa acctaaacaa
     7441 aaatagacac ttttcatggg gagagaaatc gccccaattt ataagtaata gccatgcttt
     7501 tttctacgac caaataaatc aagcgcggca aactaatcaa tttgaaaaca actaaaagaa
     7561 ccctacctaa acccctatgc tttccatcgc caaacaaaac attaagcata gggtacttgg
     7621 tataaaaaac taattgaaaa tccctttatt ttttataaat gtgaccgtgt gaccatcttt
     7681 tttatttatt atataggagt tggaaatcat ttcatctttt ctatataaat aatttttcaa
     7741 atcttggtaa cat
//
LOCUS       DBNFEG010000108         7688 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.108, whole genome shotgun sequence.
ACCESSION   DBNFEG010000108 DBNFEG010000000
VERSION     DBNFEG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.108"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(49..1455)
                     /gene="lacG"
                     /locus_tag="AC1PNG_001499"
     CDS             complement(49..1455)
                     /gene="lacG"
                     /locus_tag="AC1PNG_001499"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HIP6701998.1"
                     /translation="MSKTLPKDFIFGGATAAYQAEGATKTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEFGVNGIRISIAWSRIFPTGFGKVNEKGVEFYHNLFAE
                     CHKRGVEPFITLHHFDTPEALHSNGDFLNRENIDHFVNYAEFCFKEFTEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDLAKVFQSHHNMMVSHARAVKLFKDGGYKGEIGVVHAL
                     PTKYPFDKNNPDDVRAAELEDIIHNKFILDATYLGGYSEKTMEGVNHILSLNGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGPTEIIHNGKGEKGSSKYQIKGVGRR
                     EAPVDVPKTDWDWIIYPQGLYDQIMRVKRDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVISDAIEDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAYWYKQVAETQVIE"
     gene            complement(1521..3224)
                     /locus_tag="AC1PNG_001500"
     CDS             complement(1521..3224)
                     /locus_tag="AC1PNG_001500"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HIP6701999.1"
                     /translation="MKGLIAQIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFKWSADIETLLMTPYNYSMGILGLFVAGTTAKALTDSMNRSLPSTNQ
                     INFISTMLAAIVGFLLMAANPAKDGGFLTGFMGTKGLLTAFIAAFITVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVSVVLLYAIELLVHNALGVNVAESIGKLLAPLFQ
                     AADGYVGISIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEANLQLLQAGEHADKIL
                     TSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPL
                     VLNPIFFIPFILTPIVNVWIFKFFIDSLGMNSFTANLPWTTPAPLGIVLGTNFQVLSF
                     ILAAVLIVVDVAIYYPFLKVYDQQILEEERTGKSSDELKEKVAANFNTAKADAILEKA
                     GATETVESKEITEQTNVLVLCAGGGTSGLLANALTKAAKEYGAPVTAAAGSYGGHREI
                     LPQYQLVILAPQVASNYEDLKVETDKLGIKLAKTEGAQYIKLTRDGQGALDFVKQQME
                     N"
     gene            complement(3224..3541)
                     /locus_tag="AC1PNG_001501"
     CDS             complement(3224..3541)
                     /locus_tag="AC1PNG_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIP6702000.1"
                     /translation="MNREEITLLGFEIVSYAGDARSKFLEALKAAQEGDYAKAEELIA
                     AGGECLNDAHNAQTSLLQKEAAGEDLAFSVTLMHGQDHLMTTILLQDLMKHMIELYKR
                     GAK"
     gene            complement(3569..4549)
                     /gene="lacD"
                     /locus_tag="AC1PNG_001502"
     CDS             complement(3569..4549)
                     /gene="lacD"
                     /locus_tag="AC1PNG_001502"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HIP6702001.1"
                     /translation="MVLTAQKRSLMEKVSDKNGVISALAFDQRGALKKMMAKHQTEEP
                     TVEQIETLKTIVSEELTKYASSILLDPEYGLPASKARADEAGLLLAYEKTGYDASTTS
                     RLPDCLDDWSVKRLKEQGADTLKFLLYYDVDGDEQVNRQKQAYIERIGSECKAEDIPF
                     FLEILSYDEGIADNTGVEFAKVKPRKVNEAMKVFSDERFGIDVLKVEVPVNMNFVEGF
                     GDGEVVYTKEEAAQHFRDQEASTHLPYIYLSAGVSAKLFQDTLVFAAESGAKFNGVLC
                     GRATWAGSVQVFIEQGEEAAREWLRTTGFQNIDELNKVLEKTASPWTEKV"
     gene            complement(4552..5484)
                     /gene="lacC"
                     /locus_tag="AC1PNG_001503"
     CDS             complement(4552..5484)
                     /gene="lacC"
                     /locus_tag="AC1PNG_001503"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HIP6702002.1"
                     /translation="MILTITMNPSVDISYPLEQFKLDTVNRVAKTHKTPGGKGLNVTR
                     VLSQLGDDVIASGLLGGKIGEFIESELDKSSVKHSFYKIAGETRNCIAILHDGNQTEI
                     LEQGPTISEEEATGFLNYFEKLVPDLGIVAISGSLPKGLDTNFYSRMVGICNKYNIPV
                     VLDCSGEALLNVLDGQEKPTVIKPNTEELSQLVGTEVTDDIDSLKSVLHNSLFDGIEW
                     VIVSLGSKGAFAKHHNVFYKVSIPKIEVVNPVGSGDSTVAGITSALVHNDSDENLLKK
                     ANVLGMLNAQEALTGHVNLNNYDNLFNQIEVTEV"
     gene            complement(5496..6011)
                     /gene="lacB"
                     /locus_tag="AC1PNG_001504"
     CDS             complement(5496..6011)
                     /gene="lacB"
                     /locus_tag="AC1PNG_001504"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HIP6702003.1"
                     /translation="MKIAVGCDHIVTNDKIAVVDYLKTQGYEVIDCGTYDNIRTHYPI
                     FGKKVGEAVASGEADLGVCICGTGVGINNAVNKVPGIRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMTDIIEAFIHAEYKPTEENKKLIEKIQHVETLNQGQADPHFFDEF
                     LEKWDRGEYHD"
     gene            complement(6045..6470)
                     /gene="lacA"
                     /locus_tag="AC1PNG_001505"
     CDS             complement(6045..6470)
                     /gene="lacA"
                     /locus_tag="AC1PNG_001505"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HIP6702004.1"
                     /translation="MAVIIGADKAGTELKETVKSYLIELGKEVVDVSKDGQDFVDVTL
                     AVAKEVTSNDSNLGIVVDAYGVGPFMTATKIKGMIAAEVSDERSAYMTRGHNNSRIIT
                     IGSEIVGPGVAKNIAKGFVEGEYDGGRHQIRVDMLNKMC"
     gene            complement(6708..7658)
                     /locus_tag="AC1PNG_001506"
     CDS             complement(6708..7658)
                     /locus_tag="AC1PNG_001506"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HIP6702005.1"
                     /translation="MTKEIIGVDLGGTSVKLAILDVLGNIEDQWSIPTDISDNGKNIV
                     SDIIRSIQEYLLENSLSLGDIKGIGMGSPGKIDFEKGTVTGAYNLGWSKEQNIREQFE
                     EAFGRPFYIDNDANVAALGERWKGAGDNAPDVIFVTLGTGVGGGIIAQGQLLHGVNGS
                     AGEIGHMVVEEDGFPCTCGNIGCLETVASATGIVNIAKSFASQFDEPSELRRLILEHQ
                     VTAKDVFDYAKKNDSLGQKIVWQFANYLGKSLSQLANALNPNYIVIGGGVSAAGDFLL
                     DKVKEEFDRFAFPTVRNSTKLALATLGNDAGVIGAASLVL"
BASE COUNT         2345 a         1651 c         1210 g         2482 t
ORIGIN      
        1 acacccacac tcttatcttg cgttagaaat ttttaagaag aatattatct attcaataac
       61 ctgagtttct gctacttgtt tataccagta ggctgatttt ttcggataac gttcctgtgt
      121 ctcaaaatct acatagaaga gaccatagcg tttttcataa ccatttgacc atgagaaaac
      181 atccatcaag gaccaaataa agtatccttt aacattggct ccatcttcaa tcgcatctga
      241 aatgacttct aagtgtttct tcacgtaatc aatacgacca tcatcataga cagtattgtc
      301 tacaaattca tctttataac ccaaaccatt ttcagtgata taaattttct tataatttgg
      361 gtagtctcgt tttacacgca tgatttggtc atatagacct tgtggataaa taatccagtc
      421 ccaatccgtc ttaggtacat caactggggc ttcacggcgt ccaacacctt taatttgata
      481 tttagagcta cctttctcac ctttaccatt atgaataatt tctgttggcc catcaaaggc
      541 ttgcatccaa tcactcatgt aatagttaat accaaggaaa tcgttcaaat ctttagctgc
      601 ttctagcgct tgaaaatctt catcacgaag atcaagctca ccaccattta atgacaggat
      661 atgattgaca ccttccatcg ttttctctga gtacccacct agataagttg catcaagaat
      721 gaatttatta tggataatat cttcaagttc tgctgctctg acatcatcag gattgttttt
      781 atcaaatgga tattttgttg gtagagcatg tactactcca atttcaccct tatatccacc
      841 atctttaaac agtttcacag cacgagcatg agaaaccatc atgttatgat gtgattggaa
      901 gactttagca agatcgtatt tgatacctgg tgggaattta ccaacaagat attgtccatc
      961 accaattggt ccaatttcat taaaggttgt ccaataatta acttctgtaa attctttaaa
     1021 acagaattct gcatagttaa caaaatggtc aatattttca cggtttaaga aatcgccatt
     1081 tgaatgtaaa gcttcaggag tatcaaagtg gtgaagagtt ataaatggct caacgccacg
     1141 cttatgacac tcagcaaaaa gattgtgata gaattcaaca cctttttcat ttacttttcc
     1201 aaacccagtt gggaaaatac gggaccaagc gattgaaata cggattccat taacaccaaa
     1261 ttcttcagct aacttcaagt caacaggata gcgattatag aaatcacttg ctggctcagc
     1321 cgtataccag taattatctt ctaggtattt gtcccaagca actggacctt taccgtctgt
     1381 tttagtagca ccttcagctt gatatgctgc tgttgcacca ccgaaaataa aatctttagg
     1441 taatgttttt gacatttgtt tttcctcttc atctaaaaag ggaagttgag ttcccccaac
     1501 cttccctaat gttgtctatt ttaattttcc atttgttgtt taacaaagtc caatgcgcct
     1561 tgtccatcac gagtcaattt gatgtattgt gcaccttctg tcttagctaa tttgatacca
     1621 agtttatctg tttctacttt caagtcttca tagtttgacg ctacttgagg agcaaggata
     1681 acgagctgat attgcggcaa gatttcacgg tgaccaccat agcttcctgc tgctgcagta
     1741 actggagcac catattcttt agctgctttt gtcaaagcat ttgcaagaag accacttgtt
     1801 cctccacctg cacagagaac caaaacattt gtttgttcag taatttcttt tgattcaaca
     1861 gtttcagttg ctcctgcttt ttcaagaatg gcatcagctt ttgctgtatt gaagtttgcg
     1921 gctacttttt ctttcaactc atctgaagat ttaccagttc gttcttcttc aagaatttgt
     1981 tggtcataaa ccttcaagaa tgggtaataa atggcaacat caacaactat caaaacagca
     2041 gctaagatga atgatagaac ctggaagtta gtacctagta caattcctag gggagctggt
     2101 gttgtccaag gtaagtttgc agtaaaactg ttcattccta gactatcaat aaagaatttg
     2161 aaaatccaaa cgttaacaat tggggttaag ataaaaggaa tgaagaaaat tgggttcaat
     2221 accagtggag caccgaacaa aattggttcg ttaactccaa agaaagtagg aacaacagac
     2281 gcacgtccaa tcgctttatt acgttttgat ttcgtcaacc acataaacat gaacggaaca
     2341 accaatgttg caccagtacc acccatcgta acgatgaaca tttgagtacc tgatgtcaaa
     2401 atcttatcag catgttcccc tgcttgaagc aattgtaagt tagcttcagc attggcatag
     2461 gtaatggctg caatagctgg ctcgacaatt gaaggcccgt gaataccaac aaaccagaag
     2521 aaagcaaagg caccaaagat aatggaaata ccaacataac catctgctgc ttggaacaat
     2581 ggagctaata atttaccaat tgattccgca acatttacac ctagtgcatt atgtacaaga
     2641 agttcaatag cataaagcaa tacgactgag acagtaaatg ggattaagtc tttaaatact
     2701 tgtgaaatat tcggtggaac ctcttctggc atacggatgg taacattgtt tttcacacaa
     2761 accttgtaaa cattaaccgt aataaaggct gcaatgaagg ctgtaagcaa acctttagta
     2821 cccataaatc cagttaagaa accaccatct tttgctggat tagcagccat caacaggaat
     2881 ccaacaatgg ctgcaagcat tgtagaaatg aagttaattt gatttgtgct aggcaatgag
     2941 cggttcattg aatcggttaa tgcttttgca gttgtaccag caacaaacaa tccaagaatt
     3001 cccattgagt aattgtatgg agtcatcaaa agtgtttcaa tatcagcgct ccatttgaat
     3061 ccccaagcat taggaacata agcaattaag atgaaaatac ttgagaaaag aatgaccggc
     3121 atgccagcga taaagccatc acgaatagca cggaggtaaa tattccgaga aatcttctca
     3181 aagaaaggtt tccctttctc aatttgagca atcaaccctt tcattatttt gctcctctct
     3241 tatacaactc aatcatatgt ttcattaggt cttgaagtaa gattgtagtc atcaagtgat
     3301 cttgaccatg cattaaggta acactaaagg caagatcctc accagctgct tctttttgta
     3361 gaagacttgt ctgagcattg tgtgcatcat ttaagcactc accaccagct gcaatcaatt
     3421 cttcagcttt ggcatagtca ccttcttggg cagccttcaa tgcttctaaa aatttagaac
     3481 gtgcatctcc agcataagaa acaatttcaa atcctaaaag agtgatttct tctctattca
     3541 tgattatttc ctccgaaaat ttttatgatt atactttttc agtccatgga ctagcagttt
     3601 tttccaaaac cttgttcaat tcatcaatat tttggaatcc agttgtacgt aaccattcac
     3661 gagctgcttc ctcaccttgt tcaataaaga cttgaactga gccagcccat gttgcacgtc
     3721 cacaaagaac accattgaat ttggcacctg attctgccgc aaatactaag gtatcttgga
     3781 ataatttagc agaaacaccc gcacttaaat agatataagg taggtgtgtt gaagcttcct
     3841 ggtcacggaa gtgttgtgct gcttcttcct tagtgtagac gacttcacca tcaccaaaac
     3901 cttcaacgaa attcatattg actggaactt caaccttaag aacatcaata ccgaaacgct
     3961 catctgagaa gactttcatc gcttcattca ctttacgagg ttttactttg gcgaactcaa
     4021 cacctgtgtt gtctgcaata ccttcgtcat aagacagaat ttcaaggaag aacggaatat
     4081 cttccgcctt acattcagaa ccaatacgtt caatgtatgc ttgtttctga cggtttactt
     4141 gctcatcacc atctacatca tagtatagca aaaatttcag tgtatcggca ccctgttctt
     4201 taaggcgttt gactgaccaa tcatccaaac aatctggtaa acgactcgtt gtgcttgcat
     4261 cgtatcctgt tttttcataa gcaagtaata gtcctgcttc gtctgcacga gctttggacg
     4321 ctggtaaacc gtactctgga tcaagaagaa tagaggacgc atactttgtc aattcttctg
     4381 aaacaattgt tttcaatgtt tcgatctgtt caaccgttgg ttcttcagtt tgatgtttcg
     4441 ccatcatttt tttgagtgcc ccacgttggt caaaagccaa agctgagata acgccatttt
     4501 tatcgctgac tttttccatc aatgaacgtt tttgtgctgt taataccatt attatacctc
     4561 tgttacttct atttgattaa ataaattatc gtaattgtta agattgacat gtcctgttag
     4621 cgcttcttga gcattcaaca taccaagtac atttgctttt ttcaataaat tctcatcact
     4681 atcgttatga actaaagcag atgtaatacc agctaccgtt gagtctcctg agccaacagg
     4741 attgaccact tcaattttag ggatagatac cttatagaaa acattatgat gttttgcaaa
     4801 ggctcctttt gaaccaagtg aaacaatcac ccattcaatc ccatcgaaaa gagaattgtg
     4861 aagaactgat ttcaaactat caatatcatc agtcacttcc gttcctacta gttgagaaag
     4921 ttcctcagta tttggtttaa taactgttgg tttttcttgt ccatcaagta catttagaag
     4981 ggcctctcct gaacaatcta atacaactgg gatattatat ttattacaaa ttcccaccat
     5041 acgagaataa aagtttgtat caagaccttt tgggagacta cctgagatag ctacaattcc
     5101 taaatctgga accagttttt caaaatagtt taagaaccct gttgcttctt cttcactaat
     5161 cgtaggacct tgttcaagaa tttctgtttg atttccatca tgcaaaatgg caatacagtt
     5221 tcttgtttcc ccggctatct tataaaacga atgttttaca cttgacttat ctagttcact
     5281 ctctatgaac tctccaattt taccgcctaa caagccactt gcaatcacat catctcccag
     5341 ttgggaaaga actcgagtaa catttaagcc ctttccacca ggtgttttgt gcgttttagc
     5401 aacacgattc acggtatcta atttgaattg ttcaagcgga taagaaatgt caactgacgg
     5461 attcattgtt attgtcagaa tcataacctt acctcttagt cgtgatattc accacgatcc
     5521 catttttcta ggaattcatc aaagaagtgt ggatcagctt gtccttgatt gagagtttca
     5581 acatgttgaa ttttttcaat cagttttttg ttttcttctg ttggtttata ttctgcatga
     5641 ataaacgctt caataatatc tgtcatcaat aaaccaccag tgattttacc accaaaacca
     5701 atgacgttag cgttcaattc ttctttagca tagatagctg atgtcatatc acgaacaaga
     5761 gctgagcgaa taccaggaac tttattgaca gcattgttaa tgccgacgcc tgtaccacag
     5821 atacatacac caaggtctgc ttcaccactc gcaactgctt cacctacttt tttaccaaag
     5881 attgggtaat gcgtacgaat gttatcgtaa gtaccacagt caatcacttc ataaccttga
     5941 gttttcaaat agtctactac cgcaatttta tcgtttgtaa caatatggtc acagcctact
     6001 gcaattttca tttgttttct cctctaattt taacttttga atttttagca cattttattc
     6061 aacatatcaa cacgaatttg gtggcgacct ccatcatatt caccttcaac aaatccttta
     6121 gcgatatttt tggcaacacc aggtcctaca atttcagaac caatggtaat gatacgtgag
     6181 ttattgtgac cacgtgtcat ataagcagag cgttcatctg aaacttccgc agcaatcatc
     6241 cctttgattt tagttgctgt catgaaaggt cctacaccat acgcatcaac tacgattccc
     6301 aagtttgaat cgttagaggt cacttcttta gcaactgcta aagttacatc tacgaagtct
     6361 tgaccatctt tactcacatc aacaacttct ttaccaagtt caatcaagta tgatttaact
     6421 gtctctttaa gttctgtacc tgctttatca gcaccaataa ttactgccat tttctttctc
     6481 ctgttacatt agaaaataat tgaatgtagc tacatcgacg ataaattttt gcaaaactgt
     6541 ttgaaataac gctttcatta tttctacacc attataatac caccattaaa aacaaaagtc
     6601 aacagtttta tgttttttct tgtgtgtttt aaactatttg tgtttgattt gtttgttttt
     6661 aaaaaaaata aaaacaccaa caaacttaat atttgttggt ataactatta taaaactagg
     6721 cttgcagctc caatcactcc agcatcattc cctaaagtag ctaaagctag tttagtagaa
     6781 ttcctaactg ttggaaaagc aaatctatca aactcttctt ttactttatc taaaagaaaa
     6841 tcccctgcag cagaaacacc accaccaata acaatataat tgggattaag tgcattagcc
     6901 aattgactca atgatttacc taagtaattg gcaaattgcc atacaatttt ttggccaaga
     6961 gaatcgtttt tcttagcgta atcaaaaaca tcctttgcag ttacttgatg ttctaaaatt
     7021 aaccttctta attcacttgg ttcatcaaat tgactagcaa agctttttgc aatattgaca
     7081 atccctgtgg cgctagcaac agtctccaaa cacccaatat tcccacaagt acatggaaat
     7141 ccatcttctt caacaaccat atgaccaatc tcaccagcac ttccattaac accatgaagt
     7201 aattggcctt gagcaataat tcctccacct acacctgttc caagtgttac aaaaataaca
     7261 tcaggtgcgt tatctccagc ccccttccat cgttcaccaa gcgccgcaac attagcatca
     7321 ttatctatat aaaaaggacg accaaatgct tcttcaaatt gttctctgat attttgttct
     7381 ttcgaccacc ctaagttata agctcctgtt actgttcctt tctcaaaatc aatcttgcca
     7441 ggacttccca tgcctatgcc cttaatatcg cctaaagata agctattttc taatagatat
     7501 tcttgaatgg acctgataat gtcagataca atatttttgc cattatcact tatatctgtt
     7561 gggattgacc attgatcttc tatatttcct aaaacatcta aaatggctaa ttttactgat
     7621 gttccaccta aatcaacgcc tattatttct ttcgtcataa cttattcctc ctcagttaca
     7681 ctttcaat
//
LOCUS       DBNFEG010000109         7557 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.109, whole genome shotgun sequence.
ACCESSION   DBNFEG010000109 DBNFEG010000000
VERSION     DBNFEG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.109"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            19..3285
                     /gene="rexB"
                     /locus_tag="AC1PNG_001507"
     CDS             19..3285
                     /gene="rexB"
                     /locus_tag="AC1PNG_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HIP6702006.1"
                     /translation="MKLVYTDIRNPLTQYLTDTSAEFAKEAKRVFYIAPNSLSFEMER
                     KVLEYLPEQATFDIIVTRFGQLARYLMIDRKEAGQPLDDVGLAMIFFRVLSQFEDGDL
                     KVYGRLQRDFGFINQLVALYKELQRANMSILDLEAMDSPDKQADLVKIFLAVTDILSK
                     ESFEHQSKLAQLTGLVETGQLDEQLKNIVLVVDGFSRFSAEEEALVSALNERVSEILI
                     GVYASKKAVQVIYAEGNVYQANVDFLRQLSAQFQTKATYIGQEPVLDSIGKFSKNMEA
                     YYDYSGTMIDLTPADQEKIQLWEVVNQKEEVEQVATAIRQHIHQGARYKDILLLLGDV
                     DSYKLQIGKIFDKYDIPYYFGKAEEMSHHPLVHFVESLERLRRYRFRAEDLLNLLKSG
                     LYASISQKELDLFESYILFADMKGQAAFSRAFSVNGRADYDAEVVKEKRLVYDLAVLE
                     PLRAKIMEPLNQLFKAGPQSGATLLEKFMAFLEAIEFPKNMEKMSRNLSEVEQEKEEQ
                     VWKSFTHLLENFHQIFGKEKLKMDDFLAILQAGMQASHYRTVPATVDVVNVKSYDLIE
                     PHTAKYVYAIGMGQSNFPKVAKNTSLLTEEEMEKVNLVSASSSRFDLVSRENIKKNHA
                     AMMSLLNAATEQLVISTPQIYNEGEDSLSPYVKILKEMGLSSEERGRIKSLSPQDIGH
                     YKSLLSRLIESERPSLDNEEWEGQRAFWTVLVRHLKKKLESQGIEIPTITGDITSKKL
                     AADTLAALYPEDKPLNLSASSLTNFYNNQYLYFVRNVLRLREQESIHPTAFQHGLFLH
                     RIFERVVMDQSELDFDQKVDKAILRTRDEAEFAMFYNQDADARYTEEVLDKIARSSAT
                     ILRDNDLVEIDGQEKSFRQDKALVFDLQNGKSVHVNGTIDRLDTLQINQAVGVVDYKS
                     SDQSFSVGDFYNGLKPQLVTYLAALQELDETKDKPVFGAMYLHLQDPIIKLKDTKNLE
                     QLEGAANTSLVYKGLFLKEESLGLNHFYQTRNQLYTEDEFAVLLNHNQELYKQAAMDI
                     LAGRFAINPYTKDGRSAAGEQLKAITGFEADRHMGMARRLVKEAKRQDWMERMKGGQD
                     "
     gene            3285..6938
                     /gene="addA"
                     /locus_tag="AC1PNG_001508"
     CDS             3285..6938
                     /gene="addA"
                     /locus_tag="AC1PNG_001508"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HIP6702007.1"
                     /translation="MAFEQFLSAEEIKAVQLAEANSDKQQKRTPEQIEAIYTHGQNVL
                     VSASAGSGKTFVMVQRILDKLKRGVGIDQLFISTFTVKAAGELKERIEKNLNETIAET
                     MDMDLRRHLSAQLADLTKADIGTMDSFTQKLVTTYGYSLGISPQFRILQDETEKASLK
                     KEVFDQLFADYLEQDENGAFRKLVRNFSGNRKDNGGFRQVVYQVHDFSQSTSSPTKWL
                     KEQAVQADLYSQERIELILEQGFKEKVLDKLYQAADFFRYHVEWGRNDFGSAKYFANV
                     EEVLDLLTGLDSLDQKDLMERVEKVLLINDQSRGKGLTNANRPKDEHLIAFKEEYNAG
                     KSQIISGLRDLGQEVYELTLLKDYQIQALPMLILLRDFVLDFSQAYLDVKIKEAAFEF
                     GDIGHFAIRILEENVDIRQFFQEKYHEVMVDEYQDNNHSQERMLDLLSNGHNRFMVGD
                     IKQSIYRFRQADPMIFQEKFELYQADSQAGKLILLKENFRSQIEVLEATNAIFTRLMD
                     RQVGEIKYDETHSLVAGSPGQKIAQPKHEMEYLIYDQQDSANSSTDAEEETPLTAGEI
                     EVVAKEIIRLHNEEGADFKDITLLVQKRTHNDLIISIFEKHGIPIVADGGAASYLQSL
                     EVMIMLDTLRVINNPLNDYALVALLKSPMFRFDEDELTRISLQAGTGFFYQKMEIAQQ
                     TNGQHPELMSGELKKKIKDFLSILENWRAFAKMHSIYDLIWKIFNEKFYYDYVGALPN
                     GSKRQANLYALGLRANQFEKTGYKGLSRFIVMIDRALANDKDLADVQEFLPQNAVQLM
                     TIHKSKGLEFKYVFLMNIDKRFNLEDHYQSVIISRKNGLGIQYLADMKDKVNSPLPQV
                     RVLMNTLPYQDNLQELKIANLSEQMRLLYVALTRAEKKLYLVGKGNADKLAKKYDGKK
                     ADGVLAQSTRESVATFQDWILAIDEAFSGEDLHFKKVFVTDEDLTEEKIGKLTLKSKL
                     EDASLKDIRQSEDIAQALDQLSSVQELNERYKVAIELPSLRTPSQIKKLYEPILEQEG
                     MEVMEKYQPKRTFNLPDFSKKPKITGAQVGSAVHELMQRLDLSWLVTEDTVREALEAV
                     HAEQAIKDKINVQMILDFFDTDLGREILANTDKLHREAPFASLQTDSVSQENFVLRGI
                     IDGYLLYDDHIVLFDYKTDKYDQPSQLSQRYQAQMQLYAEALKKAYKIDRVDCHLILL
                     GGERIEVVEVKVN"
     gene            complement(6948..7556)
                     /gene="plsY"
                     /locus_tag="AC1PNG_001509"
     CDS             complement(6948..7556)
                     /gene="plsY"
                     /locus_tag="AC1PNG_001509"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIP6702008.1"
                     /translation="MLGSIPSGLWIGQTFFNINIREHGSGNTGTTNTFRILGPKAGTI
                     VFVIDFLKGTLAALLPLIFHAQGISPIVFGLMAVLGHTFPIFAQFKGGKAVATSAGML
                     PGVAPAFCLYLVIIFVTSLYLTSMVSFSSVLAAALAILGALLFPAIGIILPSYDWLFT
                     VIIVFLGSFVIIRHKENIQRILKKEENLVPFGLNLTKQKKRA"
BASE COUNT         2251 a         1457 c         1806 g         2043 t
ORIGIN      
        1 aaagcatgag aatttgatat gaaattagtc tatacagata ttcgcaatcc tctgacgcag
       61 tatttaacgg atacctcggc agaatttgct aaggaagcta agcgagtttt ttatattgct
      121 ccaaactctc tatcctttga gatggaacgc aaggtcttgg aatacctgcc agaacaggca
      181 acttttgata ttatcgtgac ccgttttggg caattggccc gctatttgat gattgatagg
      241 aaagaagcag gtcaaccgct ggatgatgtt ggtctggcaa tgattttctt tcgtgttcta
      301 tcgcaatttg aagatggcga cttgaaagtc tatggtcgtt tgcagagaga ttttggtttc
      361 attaaccagc tagttgcttt atacaaagaa ttgcaacggg caaatatgtc tattttggac
      421 ttggaagcca tggactcacc agataagcag gctgatttgg taaagatttt tctggctgta
      481 acagacattt tgtccaaaga aagctttgag catcagtcta agttagcaca gttgactggc
      541 ttggtagaaa ctggtcagtt ggatgagcag ttgaagaaca ttgtgctggt ggtagacgga
      601 ttttctcgtt tttcggcaga agaagaagct ttagtaagtg ctttaaatga acgggtgtcg
      661 gaaatcctaa ttggtgtata tgccagcaag aaagctgtgc aagtcatcta tgcggaaggc
      721 aatgtctatc aagccaacgt tgacttttta cgtcagctat cagcgcagtt ccagactaag
      781 gctacttata ttggtcaaga gccagtcttg gacagtattg gtaaattttc aaagaatatg
      841 gaagcctatt atgactacag cgggaccatg atagacttga ctccagcaga tcaagaaaaa
      901 atccagctat gggaagtcgt taaccaaaaa gaagaggtgg agcaggtagc aacggcaatt
      961 cgccaacaca tacaccaagg agctcgctat aaagacatct tgttgctctt gggtgatgtg
     1021 gatagctaca agttgcagat tggcaaaatt tttgacaaat atgatattcc ctactatttt
     1081 ggtaaggcag aggagatgag ccatcatccc ctagtccact ttgtggagtc cttggagcgc
     1141 ttgcgtcgct atcgtttccg tgcggaagac ctgctcaatc tcctcaaatc tggtttgtac
     1201 gccagcattt cccaaaagga gctggacttg tttgagtcct acatcctctt tgcggatatg
     1261 aaggggcagg ctgctttttc gagggctttt tcggtcaatg gcagagcgga ctatgacgct
     1321 gaagtcgtta aggagaaaag gcttgtctat gatttggctg tcttagaacc tctacgtgct
     1381 aagattatgg agcctttgaa tcagctgttt aaggctggac cacaatctgg agctaccttg
     1441 cttgagaaat ttatggcatt tttggaggca attgagtttc ctaaaaatat ggaaaaaatg
     1501 tctcgaaatt tgagtgaagt agagcaagaa aaagaagagc aggtctggaa aagttttacc
     1561 catcttcttg agaattttca tcagattttc ggaaaagaaa aattgaaaat ggatgatttt
     1621 ctggctattt tacaggctgg catgcaggcc agtcactatc gcaccgttcc tgcaacggtt
     1681 gatgtggtca atgtcaaatc ctatgatttg attgagccac acactgcaaa gtatgtttat
     1741 gctatcggaa tgggccagtc caattttcca aaagttgcca agaataccag tctgttgacg
     1801 gaagaagaga tggaaaaagt caatcttgtt tcggcaagtt cttcacgttt cgatttggtc
     1861 agccgagaga atattaagaa gaaccatgct gccatgatgt ctctgctaaa tgctgcgacg
     1921 gaacagttag ttatttccac tcctcagatt tataatgaag gcgaagatag tctttctcct
     1981 tatgtaaaaa ttctaaaaga aatggggctt tcgtcagaag aacgtggtcg gattaaaagt
     2041 cttagtccgc aagacatcgg tcactacaag agtctcttat cccgtttgat tgagtcagaa
     2101 agaccatctc ttgataatga agaatgggaa gggcagaggg ccttctggac agtcttggtt
     2161 cgccatttga agaagaaatt ggagtcgcaa ggcattgaaa ttccgaccat tactggtgat
     2221 attacttcta aaaagcttgc agctgatacc ttggctgctt tatatccaga ggataagccg
     2281 ctcaatcttt ctgcatccag cttgaccaat ttctacaata accaatatct ttactttgtc
     2341 cgtaatgttc ttcgcttgcg ggaacaggaa tccatccatc cgacggcctt tcaacacggg
     2401 ctcttcttgc atcggatttt tgaacgagtg gtcatggacc aatctgagct agattttgat
     2461 caaaaagtgg ataaggccat cctgcgtacg cgtgacgagg cagaatttgc tatgttttat
     2521 aaccaagatg cggatgctcg ttatacggag gaggtgctgg ataagattgc tcgttccagt
     2581 gcgactattt tgcgggataa tgatttggtt gaaattgatg gtcaggaaaa gagtttccgt
     2641 caggataagg cgctagtttt tgaccttcaa aatggcaaga gtgtgcatgt aaatggtacc
     2701 attgaccgct tggatacctt acaaatcaat caggctgttg gtgttgtgga ttacaagtcc
     2761 agcgaccaat ctttttcagt tggggatttt tacaatggtc taaaacctca gttggtaacc
     2821 tacttggcag ccttacaaga gctggatgaa accaaggata aacctgtttt tggagctatg
     2881 tatttgcatt tgcaggatcc gattatcaaa ttaaaagaca cgaagaattt agaacagcta
     2941 gaaggagctg ccaatactag cttggtttat aaggggcttt tcttgaagga ggaaagtctg
     3001 gggctcaatc atttttatca gactcgtaac caactctaca cggaagatga gtttgcggtt
     3061 ttattgaacc acaaccaaga actctacaaa caagctgcca tggacattct ggctggtcgc
     3121 tttgctatta acccttatac caaagatggg cgttcggcgg caggtgagca gctgaaggca
     3181 atcactggtt ttgaggcgga tcggcacatg ggcatggcac ggcggttggt caaggaggcc
     3241 aagcgtcaag attggatgga acgaatgaaa ggaggtcagg actaatggct tttgaacaat
     3301 ttttaagcgc agaagaaatc aaggctgtgc agctggcgga agccaattct gataagcaac
     3361 aaaaacgcac acctgagcag attgaagcca tctacacgca cggtcagaat gttctggtct
     3421 ctgcatcggc tgggtcagga aagaccttcg tcatggttca acggattttg gataagttga
     3481 agcgtggcgt agggattgat cagctcttta tctcgacctt tacagtcaaa gcggctggtg
     3541 agttgaagga gcggatcgag aaaaatctca atgaaaccat tgcggagacg atggatatgg
     3601 acttgcgtcg gcatttgtca gcccagttgg cggatttgac caaggcggat attgggacaa
     3661 tggactcctt tacgcagaaa ttggtaacga cttacggtta tagccttggc atttctccac
     3721 agtttcggat tttacaggat gagacagaaa aagcgagtct gaaaaaagag gtttttgacc
     3781 agctctttgc agattatctt gaacaggatg aaaatggtgc attccgaaaa ttggtacgca
     3841 atttttcagg caaccggaag gataatggtg gtttccgtca ggttgtttat caggtgcatg
     3901 attttagcca atcgacaagc agtccaacta aatggctgaa agagcaggct gttcaagctg
     3961 atttgtacag tcaagagcgt atagagctga tcttagagca aggctttaag gaaaaagtgt
     4021 tagacaagct ctatcaagct gcagatttct tccgctacca tgtggaatgg ggcagaaatg
     4081 actttggttc cgccaagtat tttgcaaatg tggaagaagt tttagatttg ctgacaggtt
     4141 tagatagtct ggaccagaag gacttgatgg agcgggttga aaaagtcctt cttatcaacg
     4201 accagtccag aggcaagggt ctgaccaatg ccaatcgacc aaaagatgag catttgatag
     4261 cctttaaaga agaatacaat gctggcaaga gccagattat ctctggacta cgagatttgg
     4321 ggcaggaagt ttatgaatta accctgctga aagactatca gattcaagct ttgccaatgc
     4381 tgatattatt acgtgacttt gtcttggatt tttcgcaggc ttatctggat gtcaaaatca
     4441 aggaagcagc ttttgaattt ggcgatattg ggcatttcgc tattcgtatt ttagaagaaa
     4501 atgtggacat tcgccagttt ttccaagaaa aataccacga agtcatggtg gacgagtacc
     4561 aggataacaa ccatagccaa gagcgaatgc tggacctgct gtctaacggt cacaaccgct
     4621 ttatggtggg cgatattaag cagtccatct atcgattccg tcaggcggat ccgatgattt
     4681 tccaagagaa atttgaactt taccaggcag attctcaggc tggaaaattg attttgctta
     4741 aggaaaattt ccgtagtcag attgaggtct tggaggcgac aaatgctatt ttcacgcgcc
     4801 tgatggaccg tcaagtaggg gaaatcaagt atgatgaaac ccacagtcta gtggctggta
     4861 gtcctggtca aaaaattgcc cagcccaagc atgagatgga atacttgatt tacgaccagc
     4921 aggatagcgc aaactcttca acggatgctg aggaagaaac acctctgact gctggtgaaa
     4981 tcgaggttgt tgcaaaagag attattcgtc ttcacaatga agaaggagca gacttcaagg
     5041 acatcactct cttggtgcaa aaacggacgc acaatgacct cattatatcc atttttgaaa
     5101 aacatgggat tccaattgtg gcagatggcg gagcagcctc ctatctgcaa tctttagaag
     5161 tcatgattat gctggatacc ttgcgggtta tcaataatcc actcaatgac tatgccttgg
     5221 ttgctcttct caaatcgcct atgttccgat tcgatgagga tgagctgaca cggatttcct
     5281 tgcaggctgg gacaggtttc ttttaccaga aaatggaaat agcccagcaa acaaatgggc
     5341 aacatcctga acttatgtca ggagagttga agaagaaaat caaggatttc ttatctattt
     5401 tggaaaactg gcgtgctttt gccaaaatgc attctattta tgacctgatt tggaagattt
     5461 tcaatgaaaa attctactat gactatgtcg gtgctcttcc aaatggcagc aagcgtcagg
     5521 caaatctata tgccctaggt ttgcgtgcca accagtttga aaaaacaggc tacaagggct
     5581 tgtctcgttt catcgtcatg attgaccgtg ccttagcaaa tgacaaggac ttggcagatg
     5641 tacaagagtt tctaccgcaa aatgctgttc agctgatgac cattcataag tcaaaaggtc
     5701 tagagttcaa atatgtcttc ctcatgaaca tcgataaacg attcaacttg gaagaccatt
     5761 accagtcagt catcatcagc cggaaaaatg gtctaggtat ccaatattta gcggatatga
     5821 aagataaggt gaatagccca ttacctcagg tgcgagtctt aatgaacacc cttccttatc
     5881 aagataatct ccaagagcta aaaattgcca atctttcaga acaaatgcgg ttgctttatg
     5941 ttgcactaac gcgtgccgag aagaagctgt atctggttgg aaagggcaat gctgataagt
     6001 tagctaaaaa atacgatggc aaaaaggcag atggcgtact agcccaatca actcgtgaaa
     6061 gcgtggcgac cttccaagac tggattctag ccattgatga ggccttctca ggagaagact
     6121 tgcacttcaa gaaagtcttt gtgacggatg aggatttgac agaggagaag attggaaaac
     6181 ttacattgaa gagtaagcta gaagatgcca gccttaaaga tattcgtcaa tcagaagaca
     6241 ttgctcaggc cttggaccag ttgtcttctg ttcaagagtt gaacgaacgc tataaggttg
     6301 ccattgaatt gccaagttta cggactccaa gtcaaatcaa gaaactctat gagcctattt
     6361 tggaacaaga aggcatggaa gttatggaaa aataccagcc aaaacggacc ttcaacttgc
     6421 cagatttctc caagaaacct aaaatcactg gagcccaagt cggttcagca gtgcatgaac
     6481 tcatgcaacg cttggatctg tcttggttgg tgacagaaga tacggtaaga gaagccctag
     6541 aagctgttca tgctgagcaa gcgattaaag ataaaatcaa tgttcagatg attttggatt
     6601 tctttgatac agacttagga agagaaattc tagccaatac ggacaaactc caccgagaag
     6661 caccatttgc aagtttgcag acagattctg tatctcagga aaactttgtc cttcgtggga
     6721 ttatagatgg ctacctacta tatgatgatc atattgtcct ctttgattac aagacagaca
     6781 aatatgacca accaagccaa ctcagccaac gttaccaagc tcaaatgcaa ctctatgccg
     6841 aggcattgaa aaaagcctac aagatagatc gtgtagactg ccatttgatt ttgcttggtg
     6901 gggagagaat agaagtggtc gaagtcaagg taaactaaaa gcgagtacta agctcgcttt
     6961 ttctgtttgg ttagattgag tccgaatggg acaaggttct cttctttttt caaaatccgc
     7021 tggatatttt ccttgtgacg gatgatgaca aaactgccaa gaaagacgat aatcactgta
     7081 aagagccagt cgtagcttgg caggatgatt cctattgctg gaaatagcag agctcctaag
     7141 attgctaaag ctgctgctaa gacgctagag aaggaaacca tcgaagtgag gtaaagagaa
     7201 gtcacaaaaa tgatgactag atagagacag aaagcaggtg cgacgcccgg aagcatacca
     7261 gcagatgttg caaccgcctt tccacctttg aattgggcaa agatagggaa agtgtgcccc
     7321 agtacagcca ttaacccaaa tacaatcgga gaaattcctt gggcatgaaa gatcagcgga
     7381 agcaaggctg ccaacgttcc cttgagaaaa tcaattacaa atacaatcgt accagctttt
     7441 ggacctaaaa tacggaatgt gttggttgtt ccagtatttc ccgaaccatg ttcacgaatg
     7501 ttgatattga aaaaagtttg acctatccaa agtcctgacg ggatagatcc caacaaa
//
LOCUS       DBNFEG010000110         7314 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.110, whole genome shotgun sequence.
ACCESSION   DBNFEG010000110 DBNFEG010000000
VERSION     DBNFEG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.110"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            280..1053
                     /gene="sufC"
                     /locus_tag="AC1PNG_001510"
     CDS             280..1053
                     /gene="sufC"
                     /locus_tag="AC1PNG_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIP6702009.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPSYEVTQGEILFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDEDKISVRDFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGINAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAQRLEKEGYVQVAAELGIDYKEDDI"
     gene            1070..2332
                     /gene="sufD"
                     /locus_tag="AC1PNG_001511"
     CDS             1070..2332
                     /gene="sufD"
                     /locus_tag="AC1PNG_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HIP6702010.1"
                     /translation="MTKEKIQEFSALQAEPTWLTDLRLKAFDKIAELDLPVIERVKFH
                     RWNLGDGSLATNDEIGAVPDFTAIGDNPMLVQVGSQTILEQLPVDLVEKGVVFTDFAS
                     AMDLIPDVIEKYLGVAVAYDEHKLAAYNTAYFNATAVLYVPDNVEIDQPVEALFYQDS
                     TSPVAFNKRVLIIAGKNAKLNYLERFESLGDGAATTSANIVVEVIALAGSQIKFAAID
                     RLGKHLDTYLNRRGYLGNDATIDWAIGLLNEGNVVADLDSELKGNGSHANLKVVGLSS
                     GRQVQGVDTRVTNYGHNSVGHILQHGVILESGTLTFNGIGHIVRGAKGADAQQESRVL
                     MLSDKARSDANPILLIDENEVTAGHAASIGQVDPEDMYYLMSRGLDQATAERLVVRGF
                     LGAVITEIPVKEVRDQLIAVIEEKLTKR"
     gene            2334..3560
                     /locus_tag="AC1PNG_001512"
     CDS             2334..3560
                     /locus_tag="AC1PNG_001512"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HIP6702011.1"
                     /translation="MIDFESIRKDFSILDQVVNDEPLVYLDNAATTQKPQQVLDVLED
                     YYQTDNANVHRGVHTLSERATARYEAARQKVADFIQAKSSKEILFTRGTTTSLNWVAQ
                     FAKEILQPDQEVIISVQEHHSNIIPWQQACQQIGAKLRYVPLKDGELDVDSLKSILSE
                     KTKFVSLAHVSNVLGGVVPIGEIAELVHQVGAYLVVDGAQSVPHMAVNVQELDVDFFA
                     FSGHKMLGPTGIGVLYGKEELLNLMSPVEFGGEMIDFVYEQSATWKELPWKFEAGTPN
                     IAGAIGLGAAVDYLTEIGMDAIQAHEAELVDYVFPKLQAIPGLTIYGSQDLSKRTGVI
                     AFNLDDLHPHDVATALDYEGVAVRAGHHCAQPLLRYLQVPATVRASFYIYNTKADCDK
                     LVEAIIKTKEFFNGPI"
     gene            3547..3987
                     /gene="sufU"
                     /locus_tag="AC1PNG_001513"
     CDS             3547..3987
                     /gene="sufU"
                     /locus_tag="AC1PNG_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIP6702012.1"
                     /translation="MALSRLDSLYMAVVSEHSKLPRHRGSLDGVEKLELHNPTCGDVI
                     ELSVKIEKDVITDIAFDGVGCTISTASASMMTEAVLGKEISQIQELAEIFSQMVQGQE
                     DPRQKGLGDASLLAGVAKFPQRIKCATLPWNALKKALERSDSAE"
     gene            4337..5752
                     /gene="sufB"
                     /locus_tag="AC1PNG_001514"
     CDS             4337..5752
                     /gene="sufB"
                     /locus_tag="AC1PNG_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIP6702013.1"
                     /translation="MSEERIEPTPIDLGEYKFGFHDENVELVASTGKGLNEEVIREMS
                     RIKGEPEWMLEFRLKSYETFKKMPMQTWGADLSELDFDDIVYYQKPSDKPARSWDDVP
                     DKIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEYDKHGIIFTDTDTALKEHP
                     ELFKKYFGKLVPPSDNKLAALNSAVWSGGTFIYVPKGVKLDIPLQTYFRINNEGTGQF
                     ERTLIIVDEGASVHYVEGCTAPTYSTASLHAAIVEIIAHEGAYMRYTTIQNWSDNVYN
                     LVTKRARAEKNATVEWIDGNLGAKTTMKYPAVYLEGEGARGTMLSIAFANKGQVQDTG
                     AKMIHNAPRTSSSIVSKSIARGGGEVNYRGQVTFAKNSAKSISHIECDTIIMDDISKS
                     DTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKEL
                     PMEYAVELNRLIAYEMEGSVG"
     gene            complement(6026..7267)
                     /gene="pbp3"
                     /locus_tag="AC1PNG_001515"
     CDS             complement(6026..7267)
                     /gene="pbp3"
                     /locus_tag="AC1PNG_001515"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase PBP3"
                     /protein_id="HIP6702014.1"
                     /translation="MRKFLLTLTTVIALFFTSTPILAEDFSVAAKHAIAVEVSSGKIL
                     YEQDAETKASIASISKTLSIYLVYEALAKGEITLDTMVDISDYPYSLTSNTELSNVNL
                     DARSYSVRDLLNASLITSSNSAIIALAEKIAGSEAAFVDRMKAKVQEWGITDTKIVNV
                     SGLDNTDLGENIYPGSSPEDVNMFSALDVAIIARRLILDYPQVLDITSLNAYDFGGYT
                     YYSTNQMLSDGTHARGGVDGLKTGTSNSAGSSFLATTQQAGMRIITVVLNANDGLTSP
                     ENRFVATNDLMNYVYSNFSLVTLVKKGEAFEDSKIKVFNGEKETSPVVAAADLTVVQR
                     KQTDNVPTATFTTDIKEIDAPLKAGTLVSKLQLKDQDLIGKGYISEQASVDMIIPSDM
                     KEASWPFSWWNQFVRYVNEKL"
BASE COUNT         2067 a         1598 c         1719 g         1930 t
ORIGIN      
        1 attgggttta acagtccagt ggagtgaggc tgggctcaag ccccgtaccg cttctcaacg
       61 ttagtgtcaa catctcagcg cagtggttga ttggcagatt tgttcgtgtt ttacactcca
      121 aatctgacct aatcaactgt gcgggggtgg gaaaacgaac tcttctatgg atggtcgagt
      181 tctttcccca ctgccaaggt tgagtcttat cggtgtatct agttagcttg gtcagttaat
      241 gtggtttttg aagaggacaa aaatgaaaag gagcacagca tgtcagtatt agaaatcaaa
      301 gaccttcatg ttgaaatcga aggtaaggaa atcctaaaag gtgtcaacct gacccttaaa
      361 acaggtgaaa ttgcagccat catgggaccg aacggaacag ggaaatccac cctttcagca
      421 gccatcatgg gcaatccaag ctacgaagtt acccaaggtg agattctgtt tgacggtgtc
      481 aatattttag aattagaagt cgatgaacgt gcccgtatgg gtcttttctt ggctatgcaa
      541 tacccgtcag aaattccagg gattactaat gcggaatttc ttcgtgccgc aatgaacgct
      601 ggtaaggaag acgaagacaa gatttctgtt cgtgatttca ttactaagtt ggatgaaaag
      661 atggagctcc ttaacatgaa agaggaaatg gcagagcgtt acctcaacga aggtttctca
      721 ggtggtgaga aaaaacgcaa tgagattctg caattactca tgttggaacc gacttttgcc
      781 cttcttgatg agattgactc aggattggat attgatgccc ttaaagtcgt gtcaaagggg
      841 atcaatgcta tgcgtggtga aggctttggt gccatgatta ttactcacta ccaacgcttg
      901 ctcaactaca tcactcctga tgttgtccat gtcatgatgg aaggccgtgt cgtgctttca
      961 ggcggaccag aattggcaca acgcttggaa aaagaaggtt atgtccaagt cgcagcagag
     1021 cttggcatcg actacaaaga agatgacatt taagtctttg gaggaagcta tgaccaaaga
     1081 aaagattcaa gaattttcag ccttgcaggc agaaccaact tggttgacag acctgcgtct
     1141 taaggctttt gacaaaatag cagagctgga cttgccggtt atcgagcggg tcaagtttca
     1201 ccgttggaat ctaggtgacg gaagcctagc tacaaatgat gaaatcgggg cagttccaga
     1261 ttttacagct atcggtgaca atcctatgtt ggttcaagtt ggcagccaaa cgattctaga
     1321 acagttgccg gttgaccttg tagaaaaagg tgtggtcttt acggactttg cctcagctat
     1381 ggatcttatt ccagatgtta tcgagaaata tcttggagta gctgtagctt atgatgaaca
     1441 caaattagcc gcctataaca cagcttattt taacgcaaca gctgtccttt acgtgccaga
     1501 taatgttgaa atcgaccagc cagttgaggc ccttttctac caagacagca ccagtccagt
     1561 tgcctttaac aaacgtgtcc tcatcatcgc tggaaaaaat gccaagctta attatctaga
     1621 acgctttgaa agtttgggcg acggcgcagc aacgacttca gccaatattg tcgtggaagt
     1681 aatcgccctt gctggtagcc aaatcaagtt tgcggctatt gaccgtcttg gcaagcattt
     1741 ggatacctac ctcaaccgtc gcggctatct cggaaacgat gcaacgattg actgggcgat
     1801 tggtctgctc aacgaaggaa atgtggttgc cgatttggac agtgaattaa aaggaaacgg
     1861 tagccatgcc aacctcaaag ttgtcggact gtcatcaggt cgtcaggtcc aaggtgtgga
     1921 tacgcgcgtg accaactatg gacataattc ggtcggtcac atcctgcaac atggggttat
     1981 cttagaaagc ggaaccttga ccttcaatgg catcggacac attgtccgtg gtgctaaagg
     2041 tgcggatgcc cagcaggaaa gccgtgtcct catgctgtct gacaaggctc gctcagatgc
     2101 taacccaatt ctccttatcg atgagaatga ggtcacagct ggacacgcag cctcaatcgg
     2161 tcaggtggat ccagaagaca tgtactacct catgagtcgt ggtctggacc aagcaactgc
     2221 tgaacgcttg gttgttcgcg gtttccttgg cgcagtcatt accgaaattc ctgtcaagga
     2281 agttcgtgac caattgattg ccgtgattga agaaaaacta acaaagagat aagatgattg
     2341 attttgaaag cattcgcaag gatttttcaa tcctagacca agttgttaac gatgagccgc
     2401 tggtctattt ggacaatgcg gcgaccaccc aaaaaccgca acaggtactg gatgtactgg
     2461 aagattacta ccagacagac aatgccaatg tccaccgtgg agttcatact ctgtcagaac
     2521 gggcaacggc tcgctacgag gcggctcggc agaaggtggc tgattttatc caggccaagt
     2581 ctagcaagga aattctcttt accagaggaa cgaccaccag tctcaactgg gtagctcagt
     2641 ttgcaaagga aattctccag ccagaccagg aagtgataat ctcggtccaa gagcaccact
     2701 ccaatatcat cccttggcag caagcctgtc agcaaatagg tgccaagctc cgctatgttc
     2761 cactaaaaga cggcgagttg gatgtggata gcctcaagtc catactctcc gaaaagacca
     2821 agttcgtctc cctggcccac gtatccaatg tcctaggtgg cgtggttcct attggggaaa
     2881 tagcagagct ggtgcatcaa gtcggtgcct acttggtggt ggacggagcc cagtcagttc
     2941 cccacatggc tgtcaacgtg caagaattag acgtggattt ctttgccttt tcaggtcata
     3001 aaatgttggg accgacagga atcggcgttc tctacggcaa ggaagaattg ctcaatctca
     3061 tgtcgccagt tgaattcggc ggtgaaatga ttgactttgt ctatgagcag tcagctactt
     3121 ggaaggaatt gccttggaag tttgaggcag gaacgcccaa tattgcaggt gcgattggtc
     3181 tgggagcagc cgttgattac ctgaccgaaa ttggtatgga cgctatccag gcccacgagg
     3241 cagaactggt tgactatgtc tttccaaaat tgcaggctat tccaggcttg accatttatg
     3301 gcagtcagga cctgtccaag cggacgggag ttattgcctt taacttggac gacttgcatc
     3361 cacacgatgt ggcaactgct ctggactatg aaggtgttgc tgtgcgggca ggccaccatt
     3421 gtgcccaacc gcttctcaga tatttgcagg tgcctgctac tgtacgagca agtttttaca
     3481 tttacaatac caaggctgac tgtgataagt tggtcgaggc aatcatcaag acaaaggagt
     3541 ttttcaatgg ccctatctag attagactct ctttatatgg cagttgtgtc tgaacactcc
     3601 aagttgcctc gtcaccgcgg aagtttggac ggagtggaaa agttagaact ccacaatcca
     3661 acctgcggtg atgtgattga actatcagtc aagattgaaa aagatgtgat taccgatatt
     3721 gcttttgacg gcgttggctg caccatttca accgcatcag cttctatgat gactgaagca
     3781 gtgcttggca aagaaatcag tcagattcaa gaactagcag aaatcttctc acaaatggtc
     3841 caaggtcaag aagacccacg ccagaaggga ctgggagacg cctccctcct cgcaggtgtt
     3901 gccaaattcc cccaacgcat caaatgtgcc acattaccgt ggaatgccct gaagaaagca
     3961 ctggaacgaa gtgatagtgc tgaatgaagg gtaactgcga catgagcgat tttggtcgat
     4021 attaccaaaa tcagccaatt agtaagccac ttagcaaggt tgattagaac cttgcgttac
     4081 ttggttagga actttagttc caaccaagtt tagtgtcttg aagaaagcaa tcgaacgaag
     4141 tgaagatgct tactgagcat catcgaataa acaaaaatga aaatcataca gaaaggattg
     4201 ttcagtttct gattggaaat gaaattgaac tcgccctaaa agcttttgaa aaaagataaa
     4261 tctccgcgtg tgtttgcgca cgaagtcgat ttcctgtttt tcatttgctt tcttaagggg
     4321 cttagtatct tatattatgt cagaagaaag aattgaacca accccgattg atcttgggga
     4381 atacaaattc ggtttccatg atgaaaacgt ggaactagtg gcttcaactg gtaagggttt
     4441 gaacgaagaa gtcattcgtg aaatgtcccg tatcaaaggc gagcctgaat ggatgttgga
     4501 attccgtttg aaatcctacg aaaccttcaa gaaaatgccg atgcagacct ggggtgctga
     4561 tctgtctgaa ctggattttg atgacattgt ttactatcaa aaaccgtcag ataagccagc
     4621 acgcagctgg gatgatgtac cagacaaaat caaggaaacc tttgaacgca tcggtatccc
     4681 agaagctgag cgtgcttacc tggcgggtgc ttctgcccag tacgaatcag aagtggtcta
     4741 ccacaatatg aaagaagagt acgacaagca cggcattatc tttacggata ccgacacggc
     4801 tcttaaagaa cacccagagc ttttcaaaaa atactttgga aaactcgttc cgccgtcaga
     4861 taacaaactg gctgccctca actcagcggt ttggtcaggg ggcaccttta tctacgtacc
     4921 aaaaggtgtc aagctggata ttccgcttca aacctacttc cgtatcaaca atgaaggaac
     4981 agggcagttt gaacgcaccc tcatcatcgt agatgaagga gcaagcgttc actacgtaga
     5041 aggctgtaca gctccgacct actcaacagc aagtctccat gcagcgattg tagaaatcat
     5101 tgcccacgag ggtgcctata tgcgttatac caccattcaa aactggtctg acaacgtcta
     5161 taacttggta accaaacgtg cccgtgccga gaaaaatgcc acagttgagt ggattgacgg
     5221 aaaccttggt gctaagacga ccatgaaata ccctgcggtt tacttggaag gtgaaggagc
     5281 ccgtggaacc atgttgtcca ttgcctttgc caataaaggt caagtccagg ataccggtgc
     5341 taagatgatt cacaatgccc cacgtacctc atcttctatc gtgtccaaat ccattgcccg
     5401 tggcggtgga gaagtcaact accgtggtca agtgaccttt gctaaaaact cagccaagtc
     5461 gattagccac atcgagtgtg atactattat tatggatgat atttccaagt cagacaccat
     5521 tccatttaat gaaatccaca actcacaagt ggccctcgaa cacgaagcca aagtctcaaa
     5581 aatctcagaa gaacagctct actacctcat gagccgtggc ctatccgaat ccgaagccac
     5641 agagatgatt gtcatgggct ttgtcgaacc attcaccaaa gaactcccaa tggaatacgc
     5701 agtcgaactc aacagactga ttgcctatga gatggagggt tcagtgggat agtttgccgc
     5761 gaacttgaac agctttaaag ctgttcaagc ccgtcaaact ccccactgaa gtaggagatg
     5821 aggagcgacg gcagtcgctc ttctttataa ataacatagt ctgggagact agattaccct
     5881 actgacatcg cttcctgtaa ggaagtgata tgtaataaag agttctgcag gaactctcac
     5941 catccgaggc tggagataag gagtagcggt tgtcactcta cgataaaaaa atagtatgtc
     6001 gaaaatcgtt cgacatacta tttttttata atttttcatt gacatagcgg acaaattgat
     6061 tccaccaaga gaatggccag gatgcttctt tcatatcgct agggataatc atatcgacac
     6121 tagcttgctc ggaaatataa ccttttccaa tgagatcttg atctttcagt tgtagtttgc
     6181 tgaccaaggt ccctgctttg aggggagcat caatttcttt tatatccgtt gtaaaagttg
     6241 cggtggggac attatcagtt tgttttcgtt gaacaacggt taaatctgct gctgcaacaa
     6301 caggacttgt ttctttttcg ccattgaaaa cctttatctt actatcttca aaggcttcac
     6361 ctttttttac tagtgtaaca agcgaaaagt tagaataaac atagttcatc aaatcatttg
     6421 tcgcgacaaa gcgattttct ggagaggtca gcccatcatt ggcattcaat actactgtga
     6481 taatgcgcat accagcttgt tgagttgttg caagaaaact tgaaccagcc gagtttgagg
     6541 tccctgtttt taagccgtcc acccctccac gagcgtgagt gccgtcgctc agcatttggt
     6601 tggtactata gtaagtgtag ccgccaaaat cgtaggcgtt tagcgaggta atgtcgagta
     6661 cctgaggata gtcgagtatt aatcgtcttg cgatgatagc aacatctagg gccgaaaaca
     6721 tatttacatc ttcaggactg gaacctggat aaatattttc acccaagtcg gtattatcta
     6781 gcccggatac attgacaatt tttgtatcgg taattcccca ttcttggact tttgccttca
     6841 ttcgatccac gaatgcagct tcgctaccgg ctattttttc tgccagagca ataatagcac
     6901 tattggatga tgtgattaaa gaagcgttta ataggtctct tactgaatag gaccgtgcat
     6961 ccaggtttac attacttaat tctgtattag aagtgaggct atagggataa tcagaaatat
     7021 ctaccatagt atctaaagtg atctcaccct ttgccagtgc ttcgtagacc aggtaaatgc
     7081 tcaatgtctt actgatagaa gcgatgcttg ctttggtttc ggcatcttgc tcatacaaga
     7141 ttttgccgct acttacttcg actgcgatag catgcttggc tgcaacagaa aaatcttctg
     7201 ccaaaatagg tgtggaagtg aaaaaaagag caataactgt agtgagtgtt agtaaaaatt
     7261 tacgcatgat cattcctttg gctgttattt tattctatta tatcacaaga aatg
//
LOCUS       DBNFEG010000111         7308 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.111, whole genome shotgun sequence.
ACCESSION   DBNFEG010000111 DBNFEG010000000
VERSION     DBNFEG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.111"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(99..341)
                     /locus_tag="AC1PNG_001516"
     CDS             complement(99..341)
                     /locus_tag="AC1PNG_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HIP6702015.1"
                     /translation="MKKDIHPEYRTVVFMDTTTGYKFLSGSTKKSNETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(460..1488)
                     /locus_tag="AC1PNG_001517"
     CDS             complement(460..1488)
                     /locus_tag="AC1PNG_001517"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIP6702016.1"
                     /translation="MQASSRSLRLMGTIIETRIWYPQAETILDQVEELLYLYKDRFSA
                     NDLTSELMEVNLNAGVQAVPVADDLYELIKLGKEHSLAQGSFLNITIGPLVQSWRIGF
                     SDAKLPTQEMISEKLQLINPRDIELDDEEQTVYLKKEGMAIDLGALAKGYVADKIVDF
                     LKRIGVEAGLINLGGNVLTFGQAPHNADGYWRIGIQDPQKLRGENALVLKIGEESVVT
                     SGIYERTLTVNGKTYHHILSPETGYPMESQLASLTIVSKQSVDGEIWTTRLFGQEITR
                     IYKMVEETDGIEAVLIGLDGRILVTSGLSEKVLAGEERISDSLGSPSRGGFGARVTSD
                     LASGASVL"
     gene            complement(1475..2419)
                     /locus_tag="AC1PNG_001518"
     CDS             complement(1475..2419)
                     /locus_tag="AC1PNG_001518"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIP6702017.1"
                     /translation="MTIYSKIIKKIHEYDTIIIHRHKRPDPDAIGSQVGLQKLLQKNF
                     PEKTIKVTGFEEPNLAWMAGMDVVADQDYQGALVIVTDTANTARVDDDRYTQGDFLIK
                     IDHHPNEEPYGDLLWVNTEASSCSEMITELAIQSQLELDGDIARLLYAGIVGDTGRFL
                     YPATSSRTFEIVARLRQEDFDFARMSRQMDTIDFKIAKLTGYVYDNLEVDEHGAARVI
                     LTQDILEKYGVTDADTSFIVGAPGRIGIVQSWGIFVEQTDGNYRVRLRSKYLPINEIA
                     KRHDGGGHPLASGANSYSLSENEQIYQEIQEVVRNASK"
     gene            2514..2975
                     /locus_tag="AC1PNG_001519"
     CDS             2514..2975
                     /locus_tag="AC1PNG_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HIP6702018.1"
                     /translation="MALAKIVYASMTGNTEEIADIVASKLEELGLEVQVHECTTIETE
                     EILDADLIVVASYTYSYGGDGELPDEIVDFYADLADLDLTGKVYGVCGSGDTFYDDFC
                     SAVDDFDVMLGSRGATKGAENVKVDLAAEDEDIVNLEQFATDLVAKLDTMN"
     gene            3115..3384
                     /gene="rpsN"
                     /locus_tag="AC1PNG_001520"
     CDS             3115..3384
                     /gene="rpsN"
                     /locus_tag="AC1PNG_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HIP6702019.1"
                     /translation="MAKKSKMARYQRQLELIERYADLRKSLKEKGDYQALRKLPRDSN
                     PNRLKYRDRTDGRPHAYMRKFGVSRITFRELAHLGQLPGVKKASW"
     gene            3466..3744
                     /locus_tag="AC1PNG_001521"
     CDS             3466..3744
                     /locus_tag="AC1PNG_001521"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIP6702020.1"
                     /translation="MNLDCIREQINTIDSQLVELLENRMELVDQVTAYKRATGKPVLD
                     TSRENAVLERVGKLVQKDDYRSAIQATFSDIMAQSRTYQSSKLANHEQ"
     gene            3734..4948
                     /locus_tag="AC1PNG_001522"
     CDS             3734..4948
                     /locus_tag="AC1PNG_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIP6702021.1"
                     /translation="MSNKMKQAIQLLFFSNLIGVGAGIITTLFGKILLGIGDLRSEYF
                     TFLIPFLPLAGGVIVFIYQKWGREVQAGMGLVFKAGQGEKAQISPVLIPLIISTTWLS
                     HLVGASVGREGVAVQLGASLSHWLKKHGFTHLPKDMITKIGMAAGFAGLFQTPLAASF
                     FAIEVLIVGQYSWTSLPYCLVAAFTASTTSHLLGLEKFSHTVATTSFYFTDSFKWLFI
                     ALCFGFIGNLFAWFLAQAKSISTRWLPNPYIKIAIMGVGLTVLLFFFHQGHYTGLGTN
                     LIDASLAGEQVFAFDWLLKLLLTCLCLAAGFQGGEVTPLFAIGASSGVVLAGLLGLPT
                     ELVAALGYCAVFGTATNTLLAPLFISYEVFGANILPYAIPVLAIAYLINRKQTIYGQQ
                     LRKFNNAKKPII"
     gene            complement(4969..5040)
                     /locus_tag="AC1PNG_001523"
     tRNA            complement(4969..5040)
                     /locus_tag="AC1PNG_001523"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5005..5007),aa:Arg,seq:cct)
     gene            complement(5238..7262)
                     /locus_tag="AC1PNG_001524"
     CDS             complement(5238..7262)
                     /locus_tag="AC1PNG_001524"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-anchored 5'-nucleotidase"
                     /protein_id="HIP6702022.1"
                     /translation="MKKNIRLKSSILALVAGFSVIATQAVLADELAVQIMGVNDFHGA
                     LDMTGTARLEGETVRNAGTAALLDAYMDDSQAEFEETAAETETPAESIRVQAGDMVGA
                     SPSNSGLLQDEPTVKVFNKMDVEYGTLGNHEFDEGLDEYNRIMTGEAPKKGQFNEIVD
                     NYTREAAKQEIVIANVIDKETGEIPYGWKPYAIKTIPVNDKEAKIGFIGVVTTEIPNL
                     VLKKNYEQYTFLNEAETIAKYARELAEKGVNAIVVLAHVPATSKDGVAAGEAADMIAK
                     LNEIYPEHSVDLVFAGHNHVYTNGTTGKTLIVQATSQGKAYADVRAVYDTDIADFKAV
                     PTAKIIAVAPGQKTPSPEIQAIVDEANTIVKKVTEQKIATASQATDISREVNEFKESA
                     VGNLVTSAQLAIARKSGYDVDFAMTNDGGIRADLKVQEDGTVTWGAAQAVQPFGNILQ
                     VVQMTGEQIYTALNQQYDEGEKYFLQMSGIKYIYTKADNPTEENPYKVVKAFKEDGTE
                     IVPTETYTLVINDFLFGGGDGFSIFKEAKLIGAINPDTEVFVEYLTDLEKAGQTISAT
                     IPGRKAFVEKYVEEPKAEEKEDNAGTTTDVKTPEKANDGGDSVTNQKATEQPAPSGGM
                     APISNKKTEKASGNQTLPNTGQEALGSLLISLGGLVSLGMAVSVRRKEGE"
BASE COUNT         1965 a         1617 c         1415 g         2311 t
ORIGIN      
        1 ttttatttct ataggcaaag aaaaaatggt ccactggacc ataactcgct cttttatttt
       61 caagctcgtg aaaaaacagt ccgttaagga ctgttttttt atttgagacc gtattttttg
      121 ttgaagcgat ccacacgtcc atctgcttga gtgaactttt gacgtccagt atagaatggg
      181 tgtgagtctg atgaaatttc cacacggatc aatgggtaag tttcaccttc gaattcaact
      241 gtttcgttag atttcttagt tgaaccgcta aggaacttgt agccagtagt tgtgtccatg
      301 aagacaacag tgcgatattc tggatggata tcttttttca ttttaaaatt cctttctgcc
      361 atggactctt tccgagccat agattataac catttaagtt taccaaaagt ttgttcttat
      421 ggcaagtctt tttacctatt tttttaatat tttttcaagt tatagtacgg aagcacctga
      481 agcaaggtca gaagttactc tggcgccaaa tccccctctt gaaggactgc ccaagctatc
      541 agaaattctt tcttcaccag caagaacttt ctcagaaagc cctgaggtca ctagaatcct
      601 gccatccagc ccaatcagaa ccgcttcgat accatcagtt tcttctacca tcttataaat
      661 ccgagttatt tcttgaccaa acagtcgagt cgtccaaatc tcaccgtcaa cagattgttt
      721 ggacacaatg gtcaaactag ccagctgcga ctccattgga tagccggtct caggactcaa
      781 aatatgatga taggtctttc cattcacagt aagggttctt tcgtaaatac cagaggtgac
      841 tactgattct tctcctattt ttaggacgag ggcattttca ccacgtagtt tctgaggatc
      901 ctgtatacca attcgccaat agccatctgc attgtgagga gcttgaccaa aggtcaggac
      961 gttgccaccc agattaatca gtccagcctc cacacctatt cttttcaaga agtccacaat
     1021 cttatctgct acatatcctt tagcaagagc acccagatca atcgccatac cttctttttt
     1081 caaatagact gtctgctcct catcatctaa ttcgatgtcc cttggattga tgagctggag
     1141 tttttcactg atcatttctt gggttggaag tttggcgtcg ctaaatccaa tccgccagct
     1201 ttgtacaagg ggaccaatgg taatattcaa aaaagatccc tgagccagac tgtgttcttt
     1261 gcccaattta atcaattcat acaggtcatc ggcaacagga accgcctgaa cacccgcatt
     1321 gaggttgacc tccattaatt cagaggtcaa gtcattggca gaaaaccgat ccttgtagag
     1381 atagagaagc tcttctacct gatccaaaat cgtttctgcc tgaggatacc atattctggt
     1441 ttcaatgatg gttcccataa gacgaaggga gcgactactt gcttgcattc ctcactacct
     1501 cttggatttc ttggtatatt tgctcatttt cggagagtga ataagagtta gcaccacttg
     1561 ctagtgggtg gccaccgcca tcatggcgtt tggcaatctc gttgattgga agatacttac
     1621 tgcgcaaacg gactctgtaa tttccgtcag tttgttccac aaaaatcccc caagattgaa
     1681 cgattccaat ccgtcctggc gcacctacga taaaggaagt gtccgcatcg gtcaccccgt
     1741 atttttcgag aatgtcctgt gtcaaaataa cacgcgccgc accatgttca tccacttcca
     1801 agttgtcgta gacgtaacct gtcaacttgg caatcttgaa atcgatggta tccatctggc
     1861 gagacatcct tgcaaagtca aaatcttctt ggcggagacg agcaacaatc tcaaaggtgc
     1921 gcgagctggt tgccggatag agaaagcgac ctgtatcgcc cacaattcct gcatagagta
     1981 gacgagcaat atctccatcg agttccagtt ggctttggat ggctagttcc gtaatcattt
     2041 cactgcatga gctggcttcc gtattgaccc agagcaggtc accataaggc tcttcattgg
     2101 ggtgatgatc gattttaatc aagaaatctc cttgggtata gcggtcgtca tccacacgcg
     2161 ccgtattggc agtgtctgtc acgataacca aggctccttg ataatcttgg tctgcaacta
     2221 cgtccattcc tgccatccaa gcaagatttg gttcctcaaa accggttacc ttgatggttt
     2281 tttcaggaaa attcttctgc aagagtttct gcaagccaac ttgactacca atggcatccg
     2341 gatctggtcg tttatgtcgg tgaataataa tcgtgtcgta ttcgtgaatt tttttaataa
     2401 ttttactgta aattgtcata gcaactcctt ttcatcattt tagcataaac ttcccctttt
     2461 gaaaatgatg ggagtgtggt ataatgggat tactatgtaa tttggaggaa attatggcac
     2521 tagcaaaaat agtttacgct agtatgactg gtaacacaga agaaattgct gacatcgtcg
     2581 ccagtaagct agaagagtta ggtttggaag tacaggttca cgaatgtacg actattgaaa
     2641 ccgaagaaat cttggatgcg gacctcatcg tggtagccag ctacacctat tcttacggtg
     2701 gagacggtga gcttccagat gaaattgttg atttctatgc tgacctagct gacttagatt
     2761 tgaccggtaa ggtctacggt gtttgtggtt ctggcgatac cttctatgat gacttctgta
     2821 gtgcagtaga tgactttgat gtcatgttgg gttcacgtgg tgcaactaag ggtgccgaaa
     2881 atgttaaggt agatcttgcc gctgaagatg aggatattgt taaccttgag caattcgcga
     2941 cagaccttgt tgctaaacta gatacaatga attaactgac aaacagactg gcttatcaat
     3001 aggtctagtc tgttttcttt tagcctttta ttaagattgt acacctattg cttgcaatct
     3061 taatagtttt gatatataat taactggtta agttttttga aaaaggagaa ttttatggct
     3121 aaaaaatcaa aaatggcacg ctatcagaga cagttggagt taatcgagcg ctatgcggat
     3181 ttacgtaaaa gtctcaaaga aaaaggtgac tatcaagcac ttcgaaaatt gcctcgcgac
     3241 tcaaatccaa atcgactgaa ataccgcgac aggaccgatg gccgtccgca tgcttatatg
     3301 cgaaaattcg gtgtgagtcg gattaccttc cgtgaattag cccatcttgg tcaacttcct
     3361 ggggttaaaa aagcaagttg gtaacttcct caaacaaggc tagtattctg ccttgttttt
     3421 tgctatacta aaaaacaact gaaacaatat acggaggttt ataagatgaa tttggattgc
     3481 atccgcgaac agattaacac gattgatagc caattggttg aactacttga aaatcgaatg
     3541 gagttggtcg accaagttac agcctataaa cgagcaacag gtaagcctgt tttggatacc
     3601 agccgagaaa atgctgtcct tgaaagagtt ggaaaattag ttcaaaagga tgattaccgt
     3661 tctgccattc aagcaacctt tagcgatatt atggcccaat caagaaccta tcaatcttcc
     3721 aagttagcga atcatgagca ataagatgaa acaagccatc caactccttt tcttttccaa
     3781 cttaattgga gtcggtgcag gaataatcac cacacttttt gggaaaattc tactagggat
     3841 tggagaccta cggtctgaat attttacttt tctaatcccc tttctgccac ttgcaggggg
     3901 ggtgattgtt ttcatttatc aaaaatgggg aagggaagtc caagcaggta tgggcttagt
     3961 cttcaaagct ggacaagggg agaaagcgca aatttctcca gttctcattc cactcatcat
     4021 ctctacaact tggctcagtc atcttgtcgg tgcttccgtt ggtcgcgaag gtgtagctgt
     4081 ccaacttgga gccagtctct cacattggtt aaaaaaacat ggtttcacac acttgcccaa
     4141 agacatgata acaaagattg gtatggccgc aggttttgca gggctatttc aaacaccgtt
     4201 ggctgctagt ttctttgcca ttgaggtatt aatcgttggc caatattctt ggactagtct
     4261 cccctattgt ctagttgccg ctttcacggc ttctactact tcccatttat tgggattaga
     4321 gaaattttcg cataccgtcg ctactacctc attctatttt acagatagtt ttaaatggct
     4381 atttattgcc ctttgcttcg gtttcatcgg caatttattt gcttggtttc tggcgcaagc
     4441 aaaaagcatc tcaactcgat ggcttccaaa tccttacatt aaaatagcta tcatgggagt
     4501 tggactgact gttctactat ttttcttcca tcaaggtcat tatactggtc taggaaccaa
     4561 tttgatcgat gccagtttag ctggggaaca ggtgtttgct ttcgattggc tactaaaact
     4621 cctactaacc tgtctttgtt tggctgcagg ttttcaaggg ggcgaagtta ctccgctctt
     4681 tgcgattgga gcaagttctg gggttgtttt agcaggatta ctaggtctac caactgaact
     4741 tgttgcagcc cttgggtatt gtgccgtatt tggaacagcc accaatacct tactggctcc
     4801 tctcttcatt agttatgaag tattcggcgc taatatcctg ccctatgcta ttcctgtact
     4861 tgccatagct tatcttataa acagaaaaca gactatttat ggacagcagt tgaggaaatt
     4921 caataatgca aaaaagccta tcatctaggc tttttcgtat actcttcatc tcccctgcag
     4981 gaatcgaacc tgcaactaat tcttaggagg aatttgttat atccatttaa ctaagggaag
     5041 tctgcttctc tattgtaccc cagaagagag cagattgcaa gagcaaggtt atgtaagttt
     5101 tttcaaattt ttacaaaaaa gcagaactta ctctaagatg aaatacttat tatttttatg
     5161 catttctctc atgatagtct ctaaaagaag taattctaga atgtacttcg aaagtttgtc
     5221 gtttttatgc ttgattccta ctccccttcc ttacgtctca ctgagacagc cattccgagt
     5281 gaaactaagc cacccaagct aataagaagt gagcctaggg cttcttgacc ggtatttgga
     5341 agtgtttgat ttcctgatgc tttttcagtt ttcttatttg aaataggagc catacctcca
     5401 gatggtgccg gttgctcggt tgctttctga tttgttacac tatcgccacc gtcatttgct
     5461 ttctcaggtg ttttcacatc agtagttgtc ccagcattat cttctttttc ttctgctttt
     5521 ggttcttcta cgtacttctc tacaaatgct tttctacctg gaattgttgc actaatggtt
     5581 tgacctgctt tttctaaatc agtcaagtac tccacaaata cttctgtatc tggattgata
     5641 gcaccaatca gtttagcttc tttgaaaatc gagaagccat ccccaccacc aaataagaag
     5701 tcgttgatga caagtgtata ggtttctgtc ggaacaatct ccgtcccatc ttctttgaag
     5761 gctttaacaa ccttataagg attttcttcc gttggattgt cagccttcgt gtagatatat
     5821 ttaattccag acatttgaag gaaatatttt tcaccttcat catattgttg atttaaggct
     5881 gtataaatct gctcaccggt catttgaacg acttgtagga tattcccaaa tggttgaaca
     5941 gcctgtgctg ctccccaagt aactgttcca tcttcttgga ccttcaaatc tgcccgaatc
     6001 ccgccatcgt ttgtcattgc aaagtcaaca tcataacccg atttcctagc gatagctaat
     6061 tgagccgatg ttactagatt acctacagca ctttctttaa attcattcac ttcgcgtgaa
     6121 atatctgtcg cttgactagc tgtagcaatt ttttgctctg ttactttttt aacaatggta
     6181 tttgcctcat ctacaattgc ctgaatttcc ggacttggtg ttttctgccc tggtgctact
     6241 gcaataattt tcgcagtcgg aacagcttta aagtcggcaa tatctgtatc ataaacagcc
     6301 ctaacatctg cgtaagcctt accttgtgag gtagcttgaa caatcaaggt tttgcccgtt
     6361 gtaccgtttg tatagacatg gttatgacca gcaaatacaa ggtcaactga gtgttcagga
     6421 tagatttcat ttagcttagc aatcatatct gctgcttcac cagcagccac accatccttg
     6481 cttgtagctg gaacgtgagc cagtacaact atcgcattta cacctttttc agctaactca
     6541 cgcgcatatt tagcaatcgt ctctgcctca ttcaaaaaag tgtactgctc atagtttttc
     6601 ttcaaaacaa gattaggaat ttctgtcgta actacaccaa taaagccaat cttagcttcc
     6661 ttatcattca cgggaatagt cttaatagcg tagggcttcc aaccatacgg aatttcaccc
     6721 gtttctttgt caataacgtt agcaataaca atctcctgtt tagcagcttc acgagtataa
     6781 ttatctacaa tctcattaaa ctgacctttt tttggagctt caccagtcat gatacggtta
     6841 tactcatcaa gtccctcatc aaactcatgg ttccccaaag tcccgtattc aacatccatt
     6901 ttattaaaga cttttacagt tggttcatct tgcaaaagtc cagaattcga tggacttgca
     6961 ccaaccatat ccccagcttg aacacggata gactctgcag gtgtctctgt ttctgctgct
     7021 gtttcttcaa attctgcttg tgaatcatcc atgtaagcat caagtaaagc ggcagttcct
     7081 gcattacgaa ctgtttcccc ttccaatcgc gctgtccccg tcatatcaag cgcaccatgg
     7141 aaatcattaa ctcccataat ttggacagct aattcatctg ccaaaacagc ttgtgttgca
     7201 ataacactaa aaccagctac aagagctagt atactgcttt tcaaccgaat attctttttc
     7261 ataagcaaaa caatgctcct tctatttttt tcatattgac atatctat
//
LOCUS       DBNFEG010000112         7259 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.112, whole genome shotgun sequence.
ACCESSION   DBNFEG010000112 DBNFEG010000000
VERSION     DBNFEG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7259
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.112"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            272..796
                     /locus_tag="AC1PNG_001525"
     CDS             272..796
                     /locus_tag="AC1PNG_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6702023.1"
                     /translation="MKRPIHLYIFVVLSSIASVLRVFNVFFAKYNEEAVRQLLQNFNV
                     EGLDEVYFTYMIESVNFQTNLVNKAFAVVLLLAVIATIVLLFLKKNEQASYTYLGYLF
                     VTLLFSTYAFIGEKGLSQIYTDSVMRQSVEAQAMMNYIIRVVLFAIYFGVTVFFHLRK
                     PKEKPSTAINSTDI"
     gene            complement(850..1764)
                     /locus_tag="AC1PNG_001526"
     CDS             complement(850..1764)
                     /locus_tag="AC1PNG_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIP6702024.1"
                     /translation="MKTFLRRKGIACQLLAAIFIVGINLQSPPSASANARGDDYPYTV
                     VDAVDPWRLYTRQCTSFVAFRLSTVNGFTLPPAYGDANVWGHRAKQEGYQVNMTPAVG
                     AVAWWETPMHVAWVSAVNGDTVEIEEYNYGVRYTYGRRVIPKNAVSGYIHFKNLGAGT
                     TNSQPAVAPSGTYTFTSQLAIKSQASLSSPTLDYYYAGESVRYDKVLEADGQRWISYI
                     SYSGARRYIAIQPTITQSTNNSLPASGSYTIQSYSSVRNNPSLSSPEITFYGAGSVVR
                     YDKVITAEGRTWISYISYSGARRYIAIS"
     gene            complement(1944..3632)
                     /gene="argS"
                     /locus_tag="AC1PNG_001527"
     CDS             complement(1944..3632)
                     /gene="argS"
                     /locus_tag="AC1PNG_001527"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIP6702025.1"
                     /translation="MNQKQVIAERLAAILPSLEVEAIYNLLEKPKSSEMGDIAFPAFS
                     LAKVERKAPQAIAADIVEKLDTTGFEKVVATGPYVNFFLDKAAISHQVLTDVITEKDQ
                     YGKLNIGQGRNVTIDMSSPNIAKPFSVGHLRSTVIGDALANIHEKLGYKPIRINHLGD
                     WGKQFGMLIVAYKLWGDKAAVEADPISELLKLYVRINAEAEEKPELDDEARQWFKKLE
                     DGDSEAHELWQWFRDESLVEFNRIYDKLDVTFDSYNGEAFYNDKMDEGIQILEEKGLL
                     QESKGARIVDLESYNLPPALIMKTDGATLYITRDMATAMYRKRTYDFVKSIYIVGQEQ
                     INHFKQLKAVLKEMDFDWSDDMTHVTFGLVTKDKKKLSTRKGNIILLEPTLDEAISRA
                     LTQIEAKNPDLENKEEVAHAVGVGAVKFYDLKTDRDNGYDFDLEAMVSFEGETGPYVQ
                     YAYARIQSILRKANFVPSAENDYKLADAESWDIIKHIQNFSNVVERAGDKFDPSLIAK
                     YAINLAQAFNKYYAHTRILDESPERDSRLALAYATGLVLKEALRLLGVKAPEKM"
     gene            3739..4179
                     /gene="argR"
                     /locus_tag="AC1PNG_001528"
     CDS             3739..4179
                     /gene="argR"
                     /locus_tag="AC1PNG_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIP6702026.1"
                     /translation="MNKAGRHDLIKAMIRQEKIGRQTDIQQGLEARGVVVTQTTLSRD
                     LRELGVIKIHENGRSFYSLAIEEERVNFIQLLAQYAYKVDRASFILVLHSELGEAALM
                     ANIIDAEKPETILGTLAGANTLLVICRDDVAAQQVEAEINYYLN"
     gene            4194..6734
                     /gene="mutS"
                     /locus_tag="AC1PNG_001529"
     CDS             4194..6734
                     /gene="mutS"
                     /locus_tag="AC1PNG_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIP6702027.1"
                     /translation="MATEKISPGMQQYLDIKAQYPDAFLLFRMGDFYELFYEDAVEAA
                     QILELSLTSRNKNAENPIPMAGVPYHAAQQYIDTLVELGHKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVTDSSKMGADSNYLVALDRQGVQFALSYMDVSTGQFFVTSLDDFTS
                     LCGEIRNLRARELVIGYALSEEEEQVFSNQMNLLLSFEDEVTEDVQLIDNSLTDLEKA
                     AAGKLLSYLHRTQMRDLSHLQKVVHYEIKDYLQMDYATKSSLDLLENGRTGKKHGSLY
                     WLLDETKTAMGMRLLRTWIDRPLIDLKRIENRQAIVQVFLDYFFERSDLVEALKGVYD
                     IERLASRVSFGKTMPKDLLQLSQTLGNIPAIKNILQQINEPALGNLVAGLDPIPELHA
                     LISSAIDPEAQGTITDGNIIRTGFDETLDQYRLVIREGAGWIAEIEAKEREASGINNL
                     KIDYNKKDGYYFHVTNSNLGNVPDHFFRKATLKNSERYGTEELAKIEGQMLEARDKSA
                     NLEYEIFMRIRQEVEKYIGRLQKLARTIATIDVLQAFAVVAEQQHLVCPRFTDQRELT
                     IDRGRHAVVEKVMGKQTYIPNSIHLNTDTHMQLITGPNMSGKSTYMRQLAVIVIMAQM
                     GSYVPADQAELPIFDAIFTRIGAADDLVSGQSTFMVEMMEANKAVRLATDRSLILFDE
                     LGRGTATYDGMALAQSIIEYIHDKIGAKTLFATHYHELTDLSQTLEHLENVHVSTLEK
                     DGQVTFLHKIAQGPADKSYGIHVAKIAGMPEELLQRADRILQTLENQAPTAPTTPAPI
                     VVEEPSGQIDLFADTPSHPVLDELAKLDIYNMTPMEVMMAVAELKKKI"
     gene            6987..7160
                     /locus_tag="AC1PNG_001530"
     CDS             6987..7160
                     /locus_tag="AC1PNG_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702028.1"
                     /translation="MKIILTIVYSLGVVWNILFLAVNVGRIPVINLLWPAVILVACAI
                     NLYFLWTRKQESE"
BASE COUNT         1923 a         1417 c         1792 g         2127 t
ORIGIN      
        1 gccagtagcg aatccagtag tagatggtgt agagtaggca ggctacgata gtccaaatga
       61 aaaatgtatt cagcacttta gcaagacttg gcaataacca gttaatcagt agagtgacta
      121 caaaaataat cactccgata taggtgggca atttcttcat gagttttcct cctttgaact
      181 tagtataaca aaattatatt tgcagtcaaa tgaaatatat gattgacaat ataaaaatat
      241 gagtataaaa tatagaaaaa aggaggctat tatgaaaaga ccaattcatt tgtatatctt
      301 tgttgtgctg tcatctattg cgagcgtatt gcgtgtcttt aatgtatttt ttgcgaagta
      361 caatgaggaa gctgtgcgtc aattgttgca gaattttaat gtagaggggc ttgatgaggt
      421 atattttacc tatatgatag agtctgtcaa tttccaaacg aatcttgtca ataaggcatt
      481 tgcagttgtg ttattacttg ctgtgattgc gacgattgtg ctgcttttcc tcaagaaaaa
      541 tgagcaggct tcttatacct acctgggtta tctttttgta actctcctat tttcgaccta
      601 tgcttttatt ggtgagaaag ggttgagtca aatttatact gattcagtta tgcgtcagtc
      661 agtagaggcg caggcaatga tgaattatat tattcgagtt gtcctatttg ccatttactt
      721 tggtgtgacc gtcttcttcc atctgcgtaa accaaaggaa aaaccaagca cagctattaa
      781 ttcgacagat atttaatgaa aaagcacagt ctaaaaagat tgtgctttta aatattgtct
      841 tttttgtgat taggagatgg cgatgtagcg tcgagcacct gagtaactga tatagctaat
      901 ccatgtgcga ccttcggcag taataacttt atcatagcga acgactgagc cagctccgta
      961 gaaggtgatt tcaggactgg acagacttgg attgttgcgg acactagagt aggactggat
     1021 ggtgtagcta cctgatgctg gtaggctgtt gtttgtagat tgagtaatgg taggctggat
     1081 ggcgatatag cgccgagctc cagaatagct gatatagcta atccagcgtt gtccgtcggc
     1141 ttcgagaacc ttgtcgtaac ggacgctttc gccagcataa tagtaatcga gggtaggact
     1201 agatagacta gcttgagatt tgatggccag ttggctggta aaagtatagg tgccgcttgg
     1261 ggctacggcg ggttgagagt tggttgttcc cgctccgagg tttttaaaat gaatgtagcc
     1321 gctaacggca tttttgggga taactcgcct tccgtaggta tagcgaacac cgtagttgta
     1381 ttcctcaatc tctacggtat ctccatttac tgcagaaacc catgcgacat gcatgggagt
     1441 ttcccaccag gcaacggctc cgacagcagg tgtcatatta acttgataac cttcttgttt
     1501 ggcacgatgg ccccaaacat tggcatcacc ataggcaggt ggcaatgtaa aaccgttaac
     1561 tgtgctaagt cgaaaggcta caaaggaggt gcactggcgt gtgtagagtc tccatggatc
     1621 gactgcatca actactgtgt agggatagtc atctcctcgt gcattggctg aggctgaagg
     1681 tggggattgt aaattgatac cgacaataaa aatggcggct agaagctgac aggcgattcc
     1741 ttttcttctc aagaatgttt tcattatttc tccttgtgta gtagatgtag aggtatcacg
     1801 tgtacctccc atctatatat tcgtaattga aatgaatctt tttgattttc atttgggata
     1861 aggagttgtt gtgataagga atcggaaacg agagtaggca acgtggtaga aaaaaagcct
     1921 cggctgatgg ccgaaggctt tgattacatt ttttctggtg cttttacgcc gagaagacgt
     1981 agagcttctt tgaggacaag tcctgttgcg taggcaagtg ctagacggct atcacgctct
     2041 gggctttcat ccaagatacg agtgtgtgcg tagtacttgt tgaaggcttg cgccaggttg
     2101 atagcatatt tagcaatgag ggatgggtca aatttgtcac cagcacgttc tacaacgttt
     2161 gagaagtttt ggatgtgctt gatgatgtcc cagctttctg catcagccag tttgtagtca
     2221 ttttctgcgc ttggtacaaa gtttgccttg cgtaggatag actggatgcg ggcgtatgcg
     2281 tattgtacat aaggacctgt ttcaccctcg aaggaaacca tagcttctag gtcgaagtcg
     2341 tagccgttgt cacggtcggt tttgaggtcg tagaacttaa cagcgcctac accaactgcg
     2401 tgtgctactt cttccttgtt ttcaaggtca gggtttttag cttcgatttg agtaagggcg
     2461 cgtgaaatag cttcatctag agttggctcg agtaggatga tatttccttt acgagtggat
     2521 agctttttct tgtccttggt aaccagaccg aaggtgacat gggtcatatc gtcgctccag
     2581 tcgaaatcca tttctttcaa gacagccttg agttgcttga agtggttgat ttgctcctga
     2641 ccaacgatgt agatactttt cacaaagtcg taagtgcgtt tgcggtacat agctgttgcc
     2701 atgtcacgtg tgatataaag tgttgcaccg tctgttttca tgatgagggc tggtgggaga
     2761 ttgtagcttt caaggtcaac gattctcgca cctttagatt cttgaagaag tcctttttct
     2821 tctaagattt ggatgccttc gtccatctta tcgttgtaga aagcttcgcc attatagctg
     2881 tcgaaagtta catcgagttt atcgtaaatg cggttgaatt cgaccaagct ctcatcacgg
     2941 aaccattgcc atagctcgtg tgcttctgaa tcgccatctt ccaatttttt gaaccattga
     3001 cgtgcttcgt cgtccaactc aggcttttct tccgcttcag cgttgatacg cacatagagt
     3061 ttgaggagtt ctgaaattgg gtctgcttcg accgcagcct tgtcacccca aagtttgtag
     3121 gcaacaatca acataccaaa ttgtttaccc cagtcaccca agtggttgat gcggattggc
     3181 ttgtagccaa gcttttcgtg gatgttggca agggcatcgc cgataacggt tgagcgcaaa
     3241 tgtccaactg agaatggttt agcaatgttt gggctagaca tgtcgatggt tacattgcgt
     3301 ccttgaccaa tgttgagttt accgtattgg tctttttcag taataacatc tgtcaagact
     3361 tggtgggaaa tcgcagcctt gtccaagaag aagttgacgt aaggaccggt agctacaacc
     3421 ttctcaaagc cagttgtatc gagtttttca acgatgtccg ctgcgatagc ctgtggagcc
     3481 ttgcgttcca ctttagcaag tgagaaggct gggaaggcaa tatcgcccat ttctgatgat
     3541 tttggttttt ctagcagatt gtagatagct tccacttcca aactcggaag gatggcagcc
     3601 aatctttctg caatcacttg tttttgattc atataggatt tcctccaaag ttattgattc
     3661 tattttaaca catttctagg ctaatagcgg ggaaaagtgt tataatattt ctataaatat
     3721 acattttgag gtaaaatgat gaataaagca ggacgacatg atttaataaa ggccatgatt
     3781 cgtcaagaaa aaatagggcg acaaacggat attcagcaag gtttagaggc tcgaggggta
     3841 gtggtaaccc agacaacctt gtctcgtgat ttacgtgagt tgggagtgat aaaaattcat
     3901 gaaaatgggc gttcatttta ttccttagct atcgaagaag agcgcgtgaa ttttatccaa
     3961 ttattggcac aatatgcgta taaagtagat agggctagtt ttattctggt ccttcattca
     4021 gaactgggag aagcggcctt gatggccaat atcattgatg cagaaaagcc agagactatt
     4081 ttgggaacgc tggcgggagc taatacactt ttagttattt gtcgagatga tgtagcggct
     4141 cagcaagttg aagcggagat taactactat ttgaattaga acagggagag atgatggcaa
     4201 cagaaaaaat ttctccggga atgcagcagt atctggatat aaaagcacag tatccagatg
     4261 cttttttgct tttccgtatg ggagactttt atgaattatt ttatgaagat gcggtagaag
     4321 cggcgcagat tttggaattg tccttgacca gtcggaataa gaatgcggag aatccgattc
     4381 ctatggcggg ggtaccgtat catgcggcac aacagtatat cgacacactt gttgaattgg
     4441 ggcacaaggt cgcaattgcc gagcagatgg aagatcctaa gcaggcagtc ggcgtggtca
     4501 agcgggaagt agtgcaggtc attactcctg gtacggtaac agactcgtcc aaaatgggag
     4561 cagacagtaa ctatttggtt gcgcttgacc gtcagggagt gcagttcgca ctttcttata
     4621 tggatgtgtc aacaggtcag ttttttgtga ctagtttgga tgattttact agtctttgcg
     4681 gtgaaatccg caatttacgg gcacgtgaat tggtcatcgg ctatgcttta tctgaggaag
     4741 aagagcaggt cttttcaaac cagatgaacc tcctactctc ttttgaagat gaggtgacag
     4801 aggatgtcca attgattgat aactccttga cagatttgga aaaggctgcg gcgggcaaac
     4861 tcctcagcta cctacatcgg actcagatgc gagatctgag ccacttgcaa aaggtggtcc
     4921 actatgaaat caaggactat ctgcaaatgg actatgcaac caagtccagt ctggacctgc
     4981 ttgaaaacgg tcggacaggc aagaagcatg gtagtttgta ctggttgtta gacgaaacta
     5041 agacagccat gggtatgcga ctattgcgga catggattga tcgtcccttg attgacctta
     5101 agcggattga gaatcgtcag gctatcgttc aggtttttct ggattacttc tttgaacgga
     5161 gtgacttggt tgaagcttta aaaggtgtct atgacatcga acgcttggct agccgggtat
     5221 cttttggaaa aaccatgcca aaggatctct tgcagctctc ccagactctg ggaaatatcc
     5281 cagcaatcaa gaatatcttg caacaaatca atgagcctgc tctaggcaat ttggtggctg
     5341 gattggaccc aatcccagaa ctgcacgctc tcatcagctc tgctattgac ccagaagccc
     5401 aagggaccat cacagatgga aacatcatcc gtacgggctt tgatgaaacg ctggatcagt
     5461 atcgtctggt cattcgtgag ggagcgggtt ggattgcgga gattgaggct aaggagcgtg
     5521 aagcgtctgg tatcaataat cttaagattg attacaataa aaaagacggt tactatttcc
     5581 atgttaccaa ttccaatctg ggcaatgtgc cggaccattt tttccgtaag gcgaccttga
     5641 aaaactcgga gcgctatgga acggaagagt tggctaagat tgaagggcag atgttggagg
     5701 cgcgtgataa gtctgctaat ttggagtacg agattttcat gcggattcgc caagaggttg
     5761 agaagtatat aggtcgcttg cagaagctgg ctcggaccat tgcaaccatc gatgttttgc
     5821 aggcctttgc agttgtggcg gagcagcaac atttggtttg tccacgcttt acagatcaaa
     5881 gagaactgac cattgaccgt ggtcgtcatg cggtggtgga gaaggtcatg ggcaagcaga
     5941 cctacattcc caactctatt cacttgaata ccgatacgca tatgcaactg attactggtc
     6001 caaacatgag tggtaagtct acctatatgc ggcagttggc ggttatcgtc atcatggcgc
     6061 agatgggttc ctatgtgcct gcggatcagg ctgaattacc gattttcgat gctattttca
     6121 ctcgtattgg ggcggcagac gacttggtta gtggtcaatc gaccttcatg gtcgaaatga
     6181 tggaggccaa taaggcagtc cgtctggcga cagatcggtc actcatcctc tttgatgagt
     6241 tggggcgggg aacggccacc tatgacggta tggccctggc ccagtctatc atcgagtaca
     6301 tccacgacaa gattggagcc aagactctct ttgctaccca ctaccatgag ttgacagacc
     6361 ttagccagac cttggaacac ctagagaatg tccatgtatc gaccttggag aaggacggtc
     6421 aagtcacctt cctccacaag attgcccaag gtccagctga caagtcctac gggattcatg
     6481 tggctaagat tgcgggaatg ccagaggaac tattgcagcg ggcggacagg attttgcaga
     6541 cgcttgaaaa ccaagctcct actgcaccaa ctaccccagc tccgatagtc gtggaagagc
     6601 cgtctggtca aatcgacctc ttcgcagaca cgccttctca cccagtcctc gatgaactgg
     6661 caaaactgga tatctacaat atgaccccta tggaagtcat gatggcagtg gcggagttga
     6721 agaaaaaaat ataagacaat gaaagtcagg acaattgttc tggctttttt gccgtttagt
     6781 cagtttaggt gttattctcg gaaccattat ttctctcata acctcttcta ttgtcggaca
     6841 aggaacttat ctacctcttc tggtttatct ttaccttggt atacgctttg ctatttttcc
     6901 taaaccatcg gaaaatggag cggcaaatca aggacattaa cggtcgtttg tagtataata
     6961 gcactatcat tttgttggag tgttttatga aaattatttt gacgattgtc tatagcttgg
     7021 gagtggtgtg gaatatcctc tttcttgcgg taaatgttgg cagaattcca gtcatcaact
     7081 tgctttggcc tgctgtgatt ttagtggcct gtgctattaa cctgtacttt ttatggacta
     7141 gaaaacaaga gtcagaatag gctctttttt tgatccataa atatttttta tggctggcta
     7201 taaataattt aaaatatacg aaaaagagag agcatgataa gatagtgtct atacattac
//
LOCUS       DBNFEG010000113         7235 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.113, whole genome shotgun sequence.
ACCESSION   DBNFEG010000113 DBNFEG010000000
VERSION     DBNFEG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.113"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..1080)
                     /locus_tag="AC1PNG_001531"
     CDS             complement(<1..1080)
                     /locus_tag="AC1PNG_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GBS Bsp-like repeat-containing protein"
                     /protein_id="HIP6702029.1"
                     /translation="MFVKKRTLLFLSLCASALNAQLVQADMVNAPSSQVSSSQVAETG
                     GQLSVVDEAGQIKAILSNIQGEIIGVTAQFSTETNSGITVTFSMDEQGRYVAVLDRSA
                     FAEEDQVFSLKLTAQLTDGSFQTLSDYSFEWKKDVVSAAEKPTALDNSDATPSKIEEE
                     ATSTTTSSTVNSSLTAGATSSTSNSLTRSSSNTIAGSSNGSASVPSARVSTASSSVNV
                     TQPTGTITIENRNDAQGTFDVRVTNVSSPKDIASVILPTWSQSDDLRWYEATRQSDGS
                     YKLTVNKKDHKYRTGTYTVHLYYKDSSGGLTGAGGTTTHLSEVKPTGTITIENRNDAQ
                     GTFDVRVTNVSSPKDISSVILPTWSQ"
     gene            complement(1148..2188)
                     /locus_tag="AC1PNG_001532"
     CDS             complement(1148..2188)
                     /locus_tag="AC1PNG_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIP6702030.1"
                     /translation="MKKKAIVKPLLMSGIFLALLGSATLPSGAVPVVAAETSQSTTYH
                     LTDDEKVAVREYIQAKMTIDMQEYRLAFLEGMMEEMASGSAEAAWDEEIADLKANLTA
                     EQVVVLDELEANLIGSIAQHYHYLFETLTVAGKSGREEAAAIVSKYESEDDASTPEAE
                     LAALKYAREVIVELLNKESAAIDNYIAYAEATGQELAGLLESGNSNLESITSATIGYG
                     QALATASQPKFPYDFSEMDRQIAELTASLQSKVEDKSTAKTENTGVQTSQSATNGSNE
                     QDVPGQGGGQISDVTTGKGNISEAGQKKVIPNDNAKVLPKTSGKSSLPLTVLGLITIF
                     AGWLLTKKQEEK"
     gene            complement(2205..3056)
                     /gene="rfbD"
                     /locus_tag="AC1PNG_001533"
     CDS             complement(2205..3056)
                     /gene="rfbD"
                     /locus_tag="AC1PNG_001533"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019780653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIP6702031.1"
                     /translation="MILITGANGQLGTELRYLLDERGVDYVAADVAEMDITDADKVDA
                     FFAEVKPSVVYHCAAYTAVDMAEDEGKELNYKINVTGSENVAKAAAKYCATLVYISTD
                     YVFNGDLPVGKEWQVDDQPDPQSEYGRTKRKGEEAVEKFADKFYTVRTAWVFGNYGKN
                     FVFTMQNLAETRDTLTVVNDQHGRPTWTRTLAEFMVHLVDTKQAFGYYHLSNDAAEDT
                     TWFDFATEILKDTNTKVLPVDSSQFPAKAKRPFNSTMSLDKAKATGFVIPTWQEALEA
                     FYKQEKK"
     gene            complement(3111..4157)
                     /gene="rfbB"
                     /locus_tag="AC1PNG_001534"
     CDS             complement(3111..4157)
                     /gene="rfbB"
                     /locus_tag="AC1PNG_001534"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIP6702032.1"
                     /translation="MSQFKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGN
                     KANLEAILGDRVELVVGDIADAELVDKLAAKADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETNYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLY
                     GEGKNVRDWIHTNDHSTGVWAILTKGRMGETYLIGADGEKNNKEVLELILEKMGQPKD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWTPQFTDFSQGLEETIQWYTDNQDWWKAEKE
                     AVEANYAKTQEVIK"
     gene            complement(4345..4830)
                     /locus_tag="AC1PNG_001535"
     CDS             complement(4345..4830)
                     /locus_tag="AC1PNG_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIP6702033.1"
                     /translation="MFQAFLEIAEQLNKLGITPLLMGSVGLERRTGRDWQARDLDIHV
                     PSDPRGWEAPDEERIYRADELIVLMNQLGYVLVDRHEHEFHKDGLSVEFGGLHSLPEF
                     AGVELEDLEELETVGVRYYLPTLEQYLKIYQASSKDSYRAENNNQKDFAKIAYLKEIL
                     R"
     gene            complement(4830..5423)
                     /locus_tag="AC1PNG_001536"
     CDS             complement(4830..5423)
                     /locus_tag="AC1PNG_001536"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HIP6702034.1"
                     /translation="MTENFFGKTLAARPVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGTWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDFVAYSYLVNDYWALELKPKYAFVNYA
                     DPSLDIQWENLEEAEVSEADKNHPLLKDVKPLRKEDL"
     gene            complement(5423..6292)
                     /gene="rfbA"
                     /locus_tag="AC1PNG_001537"
     CDS             complement(5423..6292)
                     /gene="rfbA"
                     /locus_tag="AC1PNG_001537"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HIP6702035.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFKDMLGDGSELGISLSYAEQPSPDGLAQAFIIGEEFIGDDNV
                     ALILGDNIYHGNGLTKMLQRAASKEKGATVFGYQVKDPERFGVVEFDADMNAISIEEK
                     PEEPKSNFAVTGLYFYDNDVVEIAKNIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRLQNVQVANLEEIAYRMGYITKEQVYELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            complement(6390..7214)
                     /locus_tag="AC1PNG_001538"
     CDS             complement(6390..7214)
                     /locus_tag="AC1PNG_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIP6702036.1"
                     /translation="MTWVTNQSVVLQALLGGLFTWFCTIMGSAVVFFFKTVSRRLLDT
                     MLGFAAGVMIAASFWSLLAPSIEYAESSYGNLAWIPAAVGFAAGGIFLRLVDAWVPHL
                     HLGNDKDKAEGGGEKDRKNLSKTALLFLAITIHNIPEGLAVGVTFGALASNYSPAAFI
                     GAIGLAIGIGIQNIPEGAALAIPIRTDGASRWKAFYWGSMSAIVEPIAAVIGAFAVTF
                     MTPILPYALSFAAGAMIFVVVEELIPESQTNGNTDIATLGLMAGFIIMMILDVALG"
BASE COUNT         1994 a         1684 c         1402 g         2155 t
ORIGIN      
        1 ctgtgaccaa gtgggtaaaa taacacttga tatatctttc ggtgatgaaa cattagtaac
       61 acgaacatcg aaggtgcctt gggcatcgtt acgattttca attgtgatgg tacctgttgg
      121 cttcacttct gataaatgag tcgttgtgcc acctgcacct gttaggccac cacttgagtc
      181 cttataatat aagtgaactg tgtaggtacc tgtacgatac ttatggtcct tcttattaac
      241 agttaactta taactgccat ctgactgacg agtggcttca taccaacgta aatcgtctga
      301 ctgggaccaa gtgggtaaaa taacacttgc tatatctttc ggcgatgaaa cattagtaac
      361 acgaacatcg aaggtgcctt gggcatcgtt acgattttca attgtgatgg tacctgttgg
      421 ctgtgtaaca ttgaccgaac tggaagctgt actgactcga gcacttggaa cacttgcaga
      481 gccattcgaa cttcctgcaa tcgtatttga agaacttctt gtcagcgaat tcgaagtact
      541 acttgttgca cctgctgtca aactagaatt aaccgttgag cttgtagtag tcgaagttgc
      601 ttcctcctca attttagacg gagttgcatc actattatcc aaagcagtag gcttctcagc
      661 cgcgcttacg acatccttct tccactcgaa actataatct gataaggtct gaaaacttcc
      721 atctgttaat tgtgctgtta acttcagcga aaagacttgg tcttcctccg caaatgcaga
      781 ccgatccaaa accgctacgt atctaccctg ctcatccatt gagaatgtga cagtaatgcc
      841 gctatttgtt tcagtgctaa actgggctgt aacaccaata atttcccctt gaatgttcga
      901 tagaatagct ttaatctgac cagcctcatc aacgacagat aactgcccgc cagtttcagc
      961 aacctgagat gaagaaactt gactagatgg ggcatttacc atatccgctt gaacaagctg
     1021 agcattcaac gcggatgcac acaaagaaag aaataataac gttctttttt tcacaaacat
     1081 ctcctttatg tatttcacat gtaaaaacaa ccatatttct caaaatagat gcaattacac
     1141 taatcttcta tttttcttct tgcttctttg tcaataacca accagcaaaa attgttatga
     1201 gtcctagtac agttaatgga agactagatt tccctgaagt ttttggcaat accttagcat
     1261 tatcatttgg gataaccttt ttctgacctg cttcggaaat attgcctttg cctgtagtta
     1321 catcagaaat ctgaccgccc ccttgacctg gaacatcctg ctcatttgag ccatttgtag
     1381 cgctttgact tgtttgtacc ccggtatttt cagtcttagc tgtactttta tcctcgactt
     1441 ttgattgtag actagcagtt agttctgcaa tctgtcggtc catttctgaa aagtcatacg
     1501 gaaattttgg ttgcgaagca gttgcaagag cttgaccata tcctatagtt gcagatgtaa
     1561 tagattctaa attagagttt ccactttcca ataatccagc taattcttgt ccagttgctt
     1621 ctgcataagc aatataatta tcaatcgctg ccgactcttt attcagtagt tcaacgataa
     1681 cttctctagc gtatttcaat gcagccaatt cggcttcagg agtacttgca tcatcttcag
     1741 actcgtactt agaaactata gccgccgctt cttctcttcc gctttttcca gcgactgtaa
     1801 gtgtctcaaa taagtaatgg taatgttggg ctatgctacc aattaaattt gcttcgagtt
     1861 catctaaaac aacaacttgc tctgctgtta agtttgcctt caagtctgca atttcttcat
     1921 cccacgcagc ttcagcacta cccgaagcca tttcttccat cattccctca agaaacgcca
     1981 atctatattc ttgcatatcg atagtcattt tagcttgaat atattcacgt acggcaactt
     2041 tttcatcatc agttaaatga taggtcgtac tttgacttgt ctctgctgct acaactggaa
     2101 cagctccgga cggtagtgta gcacttccta gtaaagccag aaaaattcct gacatcaaca
     2161 acggcttaac aatcgccttc tttttcatac acatctcctg tagactattt cttttcttgt
     2221 ttatagaatg cttccaatgc ttcttgccaa gttgggatga caaaacctgt cgcctttgcc
     2281 ttgtccaagc tcatggttga gttgaatgga cgttttgcct tggctgggaa ctggctagaa
     2341 tccactggca ataccttggt gttggtatcc ttgaggattt ccgtcgcaaa gtcaaaccaa
     2401 gtcgtatctt ctgctgcatc gtttgacaaa tggtagtaac caaatgcttg cttggtgtcc
     2461 accaagtgta ccatgaattc cgcaagggta cgggtccaag ttggacgacc gtgttggtca
     2521 ttgacaaccg tcaaagtatc gcgggtttcc gcaagatttt gcatagtgaa aacaaagttt
     2581 ttaccgtagt taccaaagac ccaagctgta cgaacagtgt agaatttgtc agcaaatttc
     2641 tcaactgctt cctcaccctt gcgcttggtg cgaccgtatt cagattgagg atccggttgg
     2701 tcatctactt gccactcttt gccgactggc aaatcaccat tgaagacata gtccgttgag
     2761 atgtaaacga gggtcgcaca gtatttagcg gctgccttgg caacattttc agagcctgtt
     2821 acgttaatct tgtagttgag ttccttgcct tcatcctctg ccatatcaac agctgtgtag
     2881 gctgcacagt ggtatacaac gcttggcttt acttctgcaa agaaggcatc tactttatct
     2941 gcatccgtga tgtccatttc tgctacgtca gcggcaacgt aatcaacacc acgctcatcc
     3001 aagagataac gcaattctgt acccaactga ccatttgcac ctgtaattaa aatcatattt
     3061 gctccttatt tcaagaaagg cttgggcaag agccctagcc tttaataatc ttatttaata
     3121 acttcctgtg tcttagcata gttggcttca acagcttctt tttcagcttt ccaccagtct
     3181 tggttatctg tataccactg gatagtctct tccaaacctt gagagaagtc tgtgaattgt
     3241 ggtgtccaac caagctcgtc acgaagtttg cttgcatcaa tagcatagcg caaatcgtgt
     3301 cctgcacggt cagtcacgtg gtcgtaagca tcttttggtt gacccatttt ttcaagaatc
     3361 aattcaagca cttctttgtt gttcttctca ccgtcagcac cgattaggta agtttcaccc
     3421 atacggcctt ttgtcaagat tgcccaaaca ccagtcgagt ggtcgttggt atgaatccag
     3481 tcacggacgt ttttaccttc accgtaaagt tttggcttaa tacctgccaa gatattggtg
     3541 atttggcgtg ggataaattt ctcgatgtgt tggtaaggac cgtagttgtt tgaacagttt
     3601 gagatagttg ctttgacacc aaatgaacgc acccatgctt tgacaatcaa gtctgaagca
     3661 gctttggttg atgagtaagg tgatgacggg ttatagttgg tttccgcagt aaatttctcg
     3721 cctggacctt cgccatgacc tggcaaatct tcacgcaaag gaaggtcacc gtatacttcg
     3781 tcggtcgata catggtggaa acggatgtcg tatttacgag ccgcttcaag caaagtgtaa
     3841 gtaccgataa agttggtatg gataaatggg cttggatcgt tgagagagtt gtcgttgtgg
     3901 ctttctgccg catagtgaac gatggcatct gccttagcag ccaatttgtc aaccaactca
     3961 gcatctgcaa tgtcgccaac aaccagctca acacggtcac caagaatagc ttccaagtta
     4021 gccttgtttc ctgcataagt aagtttatcc aaaacagtta cgtgaacgtc tggatgattg
     4081 ttatatacat agtgtacaaa gtttgaaccg atgaaaccag ctccaccagt cacgataata
     4141 tttttaaatt gagacatttt tttctccgaa aagttttttc ttttttgtgt gtcatcttag
     4201 gttgtggggt cgtaagcaac caccaaggtg tttcattact aaaccatgag cctaaggtcc
     4261 catggtttcc cccgctcctt agcaaagcaa aggagcttct cactacggcg atgacacaca
     4321 gaactgctcg cttcgctcgc ttttttacct caagatttcc ttcaagtaag caatcttagc
     4381 aaagtctttt tgattgttat tttctgcacg gtaggaatct tttgaagagg cttggtagat
     4441 tttcaggtat tgttcaagtg tcggtaggta atagcgaaca cctactgttt ctagttcttc
     4501 caaatcttct agctctacac cagcaaattc aggcagtgaa tgtaggcctc cgaattcaac
     4561 agataagccg tctttatgaa attcatgttc atgacgatca accaggacat agccaagttg
     4621 attcatcaga acaatcagct catctgcacg gtaaatccgc tcctcatctg gcgcttccca
     4681 tccccgtggg tcactgggca catgaatatc aagatccctt gcctgccaat ctcgtcctgt
     4741 ccgcctttcc agaccgacag aacccattaa caagggagtg attcctaact tgtttaactg
     4801 ctctgctatt tccaaaaagg cttgaaacat tagaggtcct cttttctcaa aggcttcaca
     4861 tctttgagga gtgggtgatt tttatctgct tctgaaactt ctgcttcttc taggttttcc
     4921 cactggatgt caaggctcgg gtcagcgtag ttgacgaagg catacttagg cttaagctcc
     4981 aaagcccagt agtcgttgac caagtagctg taagccacga aatctgacaa gacttggaaa
     5041 ccattggcta ctccgcgcgg aacgaagata ccttttgaag catcgataac ggtttggtag
     5101 gtattaccga aggtttcgcc ttcacgtaga tcgacccaag ttcccaaaac cttaccgccg
     5161 tctgctactg aaatgtattt atcccaaggc tctgcgtgaa gaccacgaag aacgttttta
     5221 cgagagaagg aaacattatt ctgcaatttt ccttctgcaa agaagctttc aggaaagccg
     5281 agaggcaaca ttttttcctt ttggaagttc tccttgaacc agccacggtt gtcgccatga
     5341 acaggaatgt cgaattctaa cattcctgga atagcttcaa ctgggcgagc tgctagtgtt
     5401 ttaccgaaaa agttttctgt cattaagctt ctccaatcaa acggagcaag tattgtccgt
     5461 attcattttt cttaagcggt tgtgccaatt catacacttg ttcttttgta atgtagccca
     5521 tgcggtaagc aatttcttcc aagttagcca cttgaacgtt ttgcaaacgt tgaaccgttt
     5581 cgatgtactg tgcagcttcc aagaggcttt catgcgtacc tgtatccaac caagcaaatc
     5641 cacggcccat caattcaact gataaatcac ctcgatctaa gtaagccttg ttgacatctg
     5701 tgatttccaa ctcgccacga ggtgaaggct tgatattttt cgcaatctcc acaacatcat
     5761 tgtcatagaa ataaagacca gtaacagcaa aattcgattt tggctcttca ggtttttctt
     5821 cgatagagat ggcattcata tcagcgtcaa actcaacaac accaaaccgt tctggatctt
     5881 ttacttgata accaaagaca gtcgctccct tttctttgct agcagcacgt tgcaacatct
     5941 tggtcaaacc atttccgtgg tagatgttat caccaagaat caaggctaca ttgtcatcac
     6001 cgataaattc ttcaccgata ataaaggctt gcgccaaacc atctggactt ggttgttctg
     6061 catatgaaag tgaaattcca agttcagaac catctcctaa catatcctta aaacgtggaa
     6121 gatcttgcgg agttgaaata atcaaaatat ctttgattcc agccaacatc aatgttgaca
     6181 atggataata gatcattggt ttatcgtaaa ttggcatcaa ctgttttgaa gctgcgcgcg
     6241 tcaaaggata caagcgtgta cctgacccac cagctagaat aatacctttc atataagagt
     6301 accttcccta aattaatatc agttcattat accatatttt taaagatatt gtcataagaa
     6361 aaaagcctgt gacgacagac tttaacaaat tatcctagag caacatccaa aatcatcatg
     6421 atgataaaac cagccattaa tcccagagtt gcaatatccg tattgccgtt tgtctgcgat
     6481 tctggaataa gctcctctac gacgacaaaa atcatggctc ctgccgcaaa ggagagggca
     6541 taaggtaaaa taggtgtcat aaaggtgacc gcaaaagccc cgatgactgc tgcaatcggc
     6601 tcgacaattg ctgacattga tccccagtaa aaagctttcc acctagatgc gccgtctgta
     6661 cgaattggaa tagctaaggc tgcaccttca ggaatatttt gtataccgat cccaatagca
     6721 agaccgattg cgccgataaa agctgctgga ctatagttag aagctagtgc accaaatgtc
     6781 acaccgaccg ccaagccttc aggaatattg tgaattgtaa tagctaagaa gagcagggct
     6841 gttttggata gattttttct atccttctca ccaccacctt ctgccttatc tttatcattt
     6901 cccaaatgca aatgcggaac ccaggcatct accaagcgta aaaaaatacc acctgctgca
     6961 aaacctactg ctgctggtat ccaagccaga ttaccgtatg aactttcagc atactcaatc
     7021 gatggggcta ataaagacca aaaagaagcc gcaatcataa caccagctgc aaagcctagc
     7081 atggtatcta gcaaacgacg actaactgtc ttaaagaaaa atacaacggc agaccccata
     7141 atcgtacaaa accatgtgaa taaaccacct agcaaagcct gtagaactac agactgatta
     7201 gtaacccaag tcataactaa catcctatcc tgttt
//
LOCUS       DBNFEG010000114         7169 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.114, whole genome shotgun sequence.
ACCESSION   DBNFEG010000114 DBNFEG010000000
VERSION     DBNFEG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.114"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            276..608
                     /locus_tag="AC1PNG_001539"
                     /pseudo
     CDS             276..608
                     /locus_tag="AC1PNG_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638881.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(656..2194)
                     /locus_tag="AC1PNG_001540"
     CDS             complement(656..2194)
                     /locus_tag="AC1PNG_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HIP6702037.1"
                     /translation="MENHRKEVSFISQSILYSVLRGSLVGIVAGLVVVAFRLAIEKLF
                     SVFTHLYSLATDNAIYLLLIGGLYLVIALLVGKLIKDEPNAKGSGIPQVEAELKGIMD
                     LNWWSVLWRKFIGGVLSIASGLMLGREGPSIQLGAVTAKGVSVLLKSSEMERRSLIAS
                     GAAAGLAAAFNAPIAGLLFVVEEVYHQFSRLVWVSALAASITANFISLHVFGLMPVLH
                     MPKDLQLLSLDQYWIYILLGVFLGLAGYVYEIVILNIQDFYTLLSKIIPLPVPYYSVF
                     AFLFIIPIGYFFSNLLGGGHHLILELPYLEQGLLTLAILILIRFVWSMISYGSGLPGG
                     IFLPILTLGSLLGLFVARFFLDIGFISQEQMLLFIVLGMAGFFAAISKAPLTAIILVT
                     EMVGSLNQLMVIGLVALSSYVMMDLLGGAPVYEAMLEKILPEEVEQQEHMTLIEIVVN
                     ETLDQIFVSELRLPDSCLITSQIHHGKSRIVKGNSQLSMGDSLLVAVPISQIHTVRRI
                     FEGD"
     gene            complement(2308..3378)
                     /locus_tag="AC1PNG_001541"
     CDS             complement(2308..3378)
                     /locus_tag="AC1PNG_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP6702038.1"
                     /translation="MKRLYSFFVGIIAIVLVLWGISYKLEADSTQGTTDKLVIYNWGD
                     YIDPELLAEFTAETGIQVDYQTFDSNESMYTKIKQGGTTYDLTIPSEYMISKMTEENM
                     LIKLDKSKIEGIENIDPRFMGLSFDKNNDYSIPYFWGTLGIVYNETMVENPPQEWEDL
                     WSEEYRDNIMLIDGVREVMGFGLQSLGYSLNSNNPTEIEKAAEHLYNLTPNVKAIVAD
                     EIKGYMIQDAAAIAVSFSGEASEMLDGNENLRYVVPSKGSNLWFDNMVIPKTAKNIDG
                     AYAFMSFMLRPENALRNAEYVGYSTPIPAAKAMLDEETQNDESFYPSEETMKKMEVYD
                     NLGAEMLGMYNDLYLQFKMYRK"
     gene            complement(3378..4148)
                     /locus_tag="AC1PNG_001542"
     CDS             complement(3378..4148)
                     /locus_tag="AC1PNG_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6702039.1"
                     /translation="MKKFANIYLTVAFLLLYLPIFYLIFYAFNEGGDMNGFTGFTLEH
                     FSSMLGDSRLMLILAQTFLLAFLSSLIATIIGTFGAIYIYQAKPRFQNALLSVNNILM
                     VAPDVMIGASFLILFTMIGFQLGFVSVLLSHIAFSIPIVVLMVLPRLKEMNADMISAA
                     YDLGATQPQMLKEIMLPYLTPAIIAGYFMAFTYSLDDFAVTFFVTGNGYSNLSVEIYS
                     RARQGISLEINALSTLVFLFSILLVVGYYFISREKEAN"
     gene            complement(4148..4948)
                     /locus_tag="AC1PNG_001543"
     CDS             complement(4148..4948)
                     /locus_tag="AC1PNG_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6702040.1"
                     /translation="MKKTSRLFAIPYALWVFLFVLAPVALIIFKSFFDIHGNFTLANY
                     QTYFNSPNMTYLRMSFNSIFYAGIITLVTLLVSYPTAYFLTKLKHRQLWLMLIILPTW
                     VNLLLKAYAFIGIFGQHGSINQFLEFIGLGAQQILFTDFSFIAVAAYIEIPFMILPIF
                     NALDDLDKNLINASRDLGATPWQTFTKVIFPLSMNGVRSGVQAVFIPSLSLFMLTRLI
                     GGNRVITLGTAIEQHFLTTQNWGMGSTIGVVLILAMLLIMWMTKERKK"
     gene            complement(4932..6086)
                     /locus_tag="AC1PNG_001544"
     CDS             complement(4932..6086)
                     /locus_tag="AC1PNG_001544"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6702041.1"
                     /translation="MKKPIIEFKNVSKVFEDSGTTVLKDISFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDASSGDIYLDGQRINDVPTNKRDVHTVFQSYALFPHMTVFENVAF
                     PLKLRKIDKAEIERRVTEALQMVRLSGYENRSIQKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGRMIEDYLVEFNGKRFEAVDGGMRPNEEVEV
                     VIRPEDLQITLPEEGKLQVKVDTQLFRGVHYEIIAYDDLGNEWMIHSTRKAIVGEVIG
                     LSFEPEDIHIMRLNETEEEFDARIEEYVEVEEVEDGLINAIEEERNEENL"
     gene            complement(6125..7033)
                     /gene="murB"
                     /locus_tag="AC1PNG_001545"
     CDS             complement(6125..7033)
                     /gene="murB"
                     /locus_tag="AC1PNG_001545"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIP6702042.1"
                     /translation="MKDKIRLELEGIDIRFDEPLKEYTYTKVGGAVDYLAFPRNRYEI
                     VRIVEFAKREGIPWQVLGNSSNIIVRDGGIRGFVIRMDKLNSVTVSGYTIEAEAGANL
                     IETTKVALFHSLSGFEFACGIPGSIGGAVYMNAGAYGGEVAHILVSAQILTPAGYVET
                     LDNRELRFGYRSSILQENGAIVLSAKFALKPGNHTVIQQEMARLTHLRELKQPLEYPS
                     CGSVFKRPLGHFAGQLIMDAGLKGYRIGGVEVSEKHAGFMVNIENGTASDYENLIAHV
                     IEVVEKSSGITLEREVRIIGDPADTL"
BASE COUNT         2309 a         1543 c         1338 g         1979 t
ORIGIN      
        1 agcaagacat ccatgatggc tccgtcctta cccacagaat aatgaaaata agcataaagc
       61 cctcactaag attatatacc tagtgagggc cttgcggttg gtacattatt gatgtgaatt
      121 atgatgacaa aactgctcca gcctactgat aagatccaca atatacactc aagaatatga
      181 ctaaaattca gtagataggc tctctaactt ataaggaagg aattgtcata gataatcatg
      241 tgtagatcaa gtcgatattg ttgtttgtat tcccctcaac ttgtccaaag cgttacttag
      301 ctacatttga tacaacgact aaaggtctac gtgcttacca cgattttctc agtcaatttc
      361 atgtggaagc tgttggtatg gaaagcaccg gtgtctattg gcgacctgtc tggcatgctc
      421 tatgtgagga cttcgagcta atacttgctc aaccagccca catgaaggcc attacaggtc
      481 agaaaactga caagaaagac gcttactgga ctgccaaatg aatgcggatt ggtctgcttc
      541 ctcggagttt cgttcccgat gaaatcattc ctgaattgag agagttgacc agacaacaga
      601 aacattataa tattgggact tttctcatct ctttgttaag acaatctaaa aaatgctaat
      661 ctccctcaaa tatcctacga acggtatgaa tttgagagat tggaactgca acgagcaagg
      721 aatcccccat agaaagttgg ctatttcctt tcacaatcct tgacttaccg tgatgaattt
      781 gactggtgat caagcatgaa tccggcaaac gtaactcgct aacaaatatt tgatccaatg
      841 tttcgttgac aacaatctct attaaggtca tgtgttcttg ctgttctact tcttctggca
      901 gaattttttc caacattgct tcatagactg gcgcgccacc taagaggtcc atcatcacat
      961 aggaactcaa agctaccaac ccgatgacca tcaattgatt caaacttcca accatttcag
     1021 ttactaaaat aatagcagtt agaggtgctt tggaaatcgc tgcgaaaaag cctgccattc
     1081 ccaatacaat aaacaatagc atctgctcct gactgatgaa accaatatca aggaagaacc
     1141 gtgcgacaaa aagcccaagg agacttccta aggttaatat gggcaaaaag atgccccctg
     1201 gtagaccaga accataggaa atcatgctcc atacaaatcg tattaaaatc aagattgcca
     1261 atgtcagtag tccttgttcc aaatatggaa gctctaaaat caaatgatgc ccgccaccca
     1321 acagattaga gaaaaaataa ccaatcggta taataaacaa aaaggcaaat acactgtagt
     1381 atggaactgg gagaggaatg atttttgaga ggagggtata gaaatcttgt atattgagga
     1441 taacaatttc atacacatat ccagcaagtc caaggaaaac tccaagtaag atatagatcc
     1501 aatattggtc aagagatagt aactgtaagt ccttcggcat atgcaaaaca ggcattaatc
     1561 caaaaacatg taaggaaata aagttcgcag tgatactggc tgccagggct gagacccata
     1621 ccagacgtga aaactgatga tagacttcct ccacaacaaa taaaaggcct gctattggtg
     1681 cattgaaagc tgctgccaaa cctgccgcag cgccactagc aatcaaactc cttctttcca
     1741 tttcactgga tttaagaaga actgatacac ctttagccgt tacagctcct aattgaatac
     1801 tgggaccttc acgccccagc attagaccag atgcaatgga taaaacaccg ccgataaatt
     1861 ttctccaaag gactgaccac cagtttaaat ccataattcc ttttagttct gcctccacct
     1921 gaggtatacc agaacctttt gcatttggct cgtctttgat caacttgcca acaagtaaag
     1981 ctatgaccag ataaagccct ccaataagaa gtaaatagat tgcattgtct gttgccagag
     2041 aatagaggtg cgtaaagaca gaaaatagtt tttcaatggc taagcgaaat gcaacgacca
     2101 ctaaaccagc gacaatgcca actaaactac cacgcagaac cgaatagagg attgattgag
     2161 aaataaaact aacttctttc cggtgatttt ccattgggat ttcccttcct ttctatagta
     2221 tgtatgacca aaaaatagga gcaataagta cttagtaaag aacctcactc ccattctttt
     2281 atttatattg ctacctgtcc aatcagacta tttccgatac atcttgaatt ggagatagag
     2341 gtcattatac attccaagca tctctgctcc aagattatca taaacttcca tcttcttcat
     2401 cgtttcttct gaaggataga aggattcgtc gttctgagtc tcctcatcca gcatagcctt
     2461 ggctgcagga attggtgttg agtagccaac atattctgca ttgcgaagcg cattttcagg
     2521 acgaagcata aaactcataa aggcgtaagc tccatcaata ttttttgctg tttttggaat
     2581 gaccatgtta tcaaaccaca ggttagaacc ttttgatggt acaacatagc gcagattttc
     2641 atttccatct aacatttcac tcgcctcacc tgagaacgaa acagcaattg ctgccgcatc
     2701 ttgtatcata taacctttta tctcatcagc aacaattgct ttgacatttg gagttaaatt
     2761 ataaagatgt tcggctgcct tttcaatctc ggtaggattg ttagaattga gactatagcc
     2821 cagtgattgt agaccaaagc ccatgacttc acgcacacca tcaattaaca tgatattgtc
     2881 acggtattcc tctgaccaca agtcttccca ttcctgagga ggattttcta ccatggtttc
     2941 attgtaaaca atgcccaagg ttccccagaa atatggaata gaatagtcat tattcttatc
     3001 aaagctaagg cccataaatc gtggatcgat attttctatc ccttcaatct tagacttatc
     3061 tagcttgatt aacatatttt cttctgtcat cttagaaatc atgtactcag atgggattgt
     3121 caaatcgtat gttgttccac cctgcttaat cttggtgtac atcgattcat tggagtcaaa
     3181 ggtctgataa tctacctgaa tacctgtttc agcagtgaat tctgcgagta attctgggtc
     3241 aatataatca ccccagttat agatgacaag tttatcagtt gtcccttggg ttgaatcagc
     3301 ttctaactta taactaattc cccataaaac taggacaata gctataattc ctacaaaaaa
     3361 tgaatacaat cgtttcatta gttggcctcc ttttcacgtg agatgaaata ataacccact
     3421 accaataaga tagagaaaag gaaaacaagg gttgataggg cattgatttc caaagaaatc
     3481 ccttgacgag cccgtgagta aatctctaca gataggtttg agtaaccatt tcccgtaaca
     3541 aagaatgtca cggcaaagtc atccaaggaa taggtaaagg ccatgaagta gcctgcaatg
     3601 atggctggtg ttaggtatgg caacatgatt tctttcagca tttgtggctg agttgccccc
     3661 aaatcataag ctgctgaaat catatcggca ttcatttcct tcaagcgtgg caaaaccatc
     3721 aatactacga ttggaataga aaaagcaata tgactgagca atactgagac aaagccaagc
     3781 tggaaaccaa tcatggtaaa gagaattagg aaactcgccc caatcatgac gtctggagct
     3841 accatcaaga tattattgac agacaagagt gcattttgaa aacgaggttt tgcctgatat
     3901 atatagatgg caccgaaagt tccgataatg gtcgcaatca aagaactcaa gaaggccaac
     3961 aagaaggttt gagccagaat cagcattagt cggctatcgc caagcataga tgaaaaatgc
     4021 tctaaggtaa agcctgtaaa gccattcata tctccgcctt cattaaaggc ataaaaaata
     4081 agatagaaaa tcggtaagta gagaaggaga aaggcaactg ttaagtagat atttgcaaat
     4141 ttcttcatta tttcttcctc tctttcgtca tccacataat caagagcatg gccaaaatca
     4201 agaccacacc tatggttgaa cccattcccc agttttgtgt tgtgagaaag tgttgttcta
     4261 tagctgtacc aagcgtgatc actcggttac cgccaatcaa acgggtcaac atgaagaggc
     4321 tcagacttgg aatgaaaacc gcctgtacac cagaacggac accattcata gaaagaggaa
     4381 aaataacttt tgtgaaggtc tgccaaggag ttgcccccaa atcacgactg gcattgatta
     4441 ggtttttatc caaatcatcc aaggcgttaa agatcggcaa aatcataaac ggaatctcga
     4501 tataggctgc aacagcaata aatgagaaat ccgtgaaaag aatttgctga gctcctagac
     4561 caataaattc taggaactgg ttgatagagc cgtgttgtcc aaaaataccg ataaaggcat
     4621 aggctttcag taaaagattg acccaggttg gcaagataat caacatgagc cagagctgtc
     4681 tatgcttgag tttggtcaag aaataggctg ttgggtagga aaccaaaagc gtgaccagag
     4741 taataatacc tgcgtaaaaa atggagttaa aactcattcg cagataggtc atattgggcg
     4801 agttaaaata ggtctggtaa ttggccaaag tgaaattgcc atgaatatca aagaaagatt
     4861 tgaagataat caaggcaact ggagcgagta caaagagaaa gacccatagg gcatagggaa
     4921 ttgcaaaaag cctagaggtt ttcttcattg cgttcctcct caatggcatt aatcaagcca
     4981 tcttctactt cttccacttc cacatattct tcgatacggg catcgaattc ttcctctgtt
     5041 tcattgagac gcatgatatg aatatcttct ggctcaaaac ttagtccaat cacttctccg
     5101 acaatagcct tacgagttga gtgaatcatc cactcattac cgaggtcgtc ataggcgatg
     5161 atttcatagt gaaccccacg gaagagttga gtatccactt ttacctgcaa tttgccttcc
     5221 tcaggtaggg taatctgcaa atcttctgga cggataacaa cttcaacttc ttcattagga
     5281 cgcataccgc catcgacagc ttcaaagcgt ttgccgttaa actcaaccag atagtcttct
     5341 atcatacgac ctggaaggat attggattca ccgatgaagg tagcaacaaa atggttgatt
     5401 ggctcgtcgt aaatgtccac tggcgtgcca gactgaacaa tttcaccttc attcatgaca
     5461 aaaatccagt cactcatggc cagagcttct tcttggtcat gggtaacgaa gacgaaggta
     5521 attcctagac gttgttgcaa ttcacgcagt tcgtactgca tttctgttct tagtttcaag
     5581 tccagggcag aaagtggttc atctagcaag acaacgcgag gctggttgat aatcgcacgt
     5641 gcaatagcaa cccgctggcg ttgaccacca gacaatttct gaatggaacg attttcataa
     5701 ccagaaagtc ggaccatttg taaggcttct gtcacacggc gttcaatttc agccttgtca
     5761 atctttcgta atttaagagg aaaagccaca ttttcaaaga cagtcatatg tgggaaaagg
     5821 gcataggact ggaaaacagt atggacatcc cgtttattgg tcggaacatc attaatccgt
     5881 tgaccgtcca gataaatatc acctgaactc gcatccaata aaccagcaat aatgttcaaa
     5941 attgttgatt tccctgaacc agaagctcct aaaagggtat aaaatttccc ctcttccaat
     6001 tcaaaactaa tatcctttaa tacagttgtt ccactgtctt caaatacttt tgaaacattc
     6061 ttaaattcaa taattggctt tttcaattct cataaattcc ttcttttttg taagagcctg
     6121 atggctagag tgtatcagca ggatcgccga taatacgaac ctctctctcc agagtaattc
     6181 ctgaagattt ttcaacaact tcaattacat gggcaatcaa attttcgtaa tcagatgctg
     6241 taccattttc tatattgacc ataaagccag cgtgtttttc agaaacttcg actccaccaa
     6301 ttcgataacc ttttaaaccc gcatccataa tcaactgtcc tgcgaagtga ccaagtggac
     6361 gcttgaagac tgaaccacaa gatggatatt ccaaaggttg cttgagctcg cgtaaatgtg
     6421 tcaaacgagc catttcttgt tgaataaccg tatgatttcc tggcttgagg gcaaattttg
     6481 cagacaaaac aatcgcaccg ttttcctgca aaatagaaga acggtagcca aatcgtaact
     6541 cacgattgtc caatgtttcg acataacctg ctggagtcaa aatctgcgct gaaactagaa
     6601 tgtgtgccac ttcacctccg taagcacctg cattcatgta gacagcacca ccaatgcttc
     6661 ccggaatacc acaagcaaat tcaaatcctg ataaagaatg gaaaagtgcg acttttgttg
     6721 tttcaatcag attggcacct gcttctgctt caatggtgta tcctgataca gtaacggagt
     6781 taagtttatc catacggata acaaaaccac gaataccgcc atctcggaca atgatattac
     6841 ttgaatttcc taatacttgc catggaatac cttcgcgttt agcaaattct actatacgaa
     6901 caatctcgta acgattccgc ggaaaagcta gataatcaac agctcctcct acttttgtat
     6961 aggtatattc ttttaaaggc tcatcgaatc gaatatcgat gccttctaat tcaagcctaa
     7021 ttttatcttt cataaccttc tcatttcaat ttaagatata caggatttat tataccaaaa
     7081 attacaggag aaaacgaata aaattcaaca aaaatacttt cacaaaccaa attaatttgc
     7141 cccaatattt tgaattggag aatggaatg
//
LOCUS       DBNFEG010000115         7161 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.115, whole genome shotgun sequence.
ACCESSION   DBNFEG010000115 DBNFEG010000000
VERSION     DBNFEG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.115"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..357
                     /locus_tag="AC1PNG_001546"
     CDS             <1..357
                     /locus_tag="AC1PNG_001546"
                     /inference="COORDINATES: protein motif:HMM:NF040303.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HIP6702043.1"
                     /translation="DELEKLKKQVQYDLLKESGCVEKLCEWDEVLPIIADVFYQNEGT
                     ISINQMRLPIDFVKERFRQLNMHHIDYICECLSKVDTNIRNIRAYILTTAFNAPQTIG
                     AYYSNQVKQDFGGSIL"
     gene            354..2948
                     /locus_tag="AC1PNG_001547"
     CDS             354..2948
                     /locus_tag="AC1PNG_001547"
                     /inference="COORDINATES: protein motif:HMM:NF019142.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HIP6702044.1"
                     /translation="MISKITKFCQRKLTIGLVSLSIGFISVNSIILSPNNLFTVYANE
                     VVSHRNDITGSSKEWGTLAGNQFIIDYTNTSFEVSKAAHSSFSENYHAIRIPETYPYK
                     LFVNGQPKTLSDLKRDKIGHLTAVIDGREVPLLDYGMVEYQMNRYLGKDIYNIYGNLS
                     YTAYAFLTTRTSHHGYFELTEADNVRDVSGQLVTGFFQTPEYRDMASAYHKSNSGYLK
                     VQVGDQELSTNIVNGRLNGLKTAKQHFYLVSNSEFSMDPQTGKPMKQLRELTEDEVHQ
                     LKVTETKVNSEIEFDVNGKTYQTTGRLNFWKNSTINPLKTRLAVMSIGDGNNIHGFDL
                     YASNNNYIEVVEKIAEEPSIPESISKMIEFVTEDGEKVSDGPSITGNKGEEVTLEITP
                     PDGYTFVSIPPERIVLEDSNPIQIIVNENTGQETLERVEEIDYRVIRQENPDMYVGDE
                     AIVRKGIRGTKTIQDIFETKKGIRTDKLLSSSVISETAPSDEIIHYGTKAIEGEEIIT
                     RTEITPFNIIEEVDPNLYEGEIVTKVEGQNGEFTYYDYYRTIKGTKELAAFKSEAKEI
                     KPKIDKVVRIGTKQIEGEIIEKSSEKIPFGMIIEEDNSLFEDERVVIREGVPGIVDVV
                     ATYKTSKGEKIGEPINVQSFIQREAVDELVKVGTKPLNTTSKVENLNEIPFTVVKEYD
                     DSLEEGVEKIIKVGKNGQKKIIVTTNYVRGIQDGESIVEEITIEEPQNQVILVGTKTL
                     QKESPSPNNDNTDSQTKIDIPSSDDNLPKVPKIPNNTQSDIEKPIEKETKDSSEINQK
                     GSSQTDVNKSKTTTKLDSNTTNNTNGSKLPETGETLVSPLLIIISSIFCLFTSLGMII
                     WKGRKSND"
     gene            3010..3435
                     /locus_tag="AC1PNG_001548"
     CDS             3010..3435
                     /locus_tag="AC1PNG_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HIP6702045.1"
                     /translation="MIIAALKLVIKEAESEKLSVKQYISQKSLQGEVPLKDLVGKGTL
                     ENLPMKQPELLELKKELNKHGVRFSVLRNKENKQFEVFFQAKDTAVLEHSFKQAVERA
                     ERKTKIKQSTLKKIQQYQKSLGQHIIKPKIKQKHQEQSL"
     gene            3477..5234
                     /locus_tag="AC1PNG_001549"
     CDS             3477..5234
                     /locus_tag="AC1PNG_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HIP6702046.1"
                     /translation="MLNVFPFGDRRLLAKSLLLYLLFSYLGTCLGHHVNNFSDGDFLD
                     RLFQAILEIETMRWIFFPSLDEIITGLTLSVVVGIVLYFRKKNAKKFRNGVEYGSARW
                     GDRKDIQPYEDPVFENNILLTDSEKLTMNSRPSNPKFARNKNILVVGGSGSGKTRFFV
                     KPNLMQMHSSYCVTDPKGTIVLECGKMLEKNGYQIKILNTINFKKSMKYNPFAYLKSE
                     KDILKLVQTIIANTKGEGEKAGEDFWVKAEKLYYTALIGYIYYESPKEEQNFNTLLAM
                     IDASEVREDDENFKNAIDYLFLALERRKPNHFAVKQYKKYKLAAGKTAKSILISCGAR
                     LAPFDIQELRELMSDDELELDTLGDRKTALFVIISDTDDTFNFVVSILYSQLFNLLCD
                     KADDVYGGRLPVHVRCLLDEFANIGLIPRFEKLIATIRSREISAAIILQAKSQLKAIY
                     KDNADTIIGNCDTELFLGGKEGTTLKELAENLGKETIELYNTSETRSNQKSFGLNYQK
                     LGKELMSRDELKVMDGGKCILEIRGTRPFYSDKYDITKHKHYKLLEDFDKRNKFDIEA
                     YLKNRGQAKLTENTSIIQL"
     gene            5310..5618
                     /locus_tag="AC1PNG_001550"
     CDS             5310..5618
                     /locus_tag="AC1PNG_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIP6702047.1"
                     /translation="MEHEMININAHLVKDVIFSSFETEDGIVPVVNFSLVKKYGKGKE
                     YTNCSAYGDKVEIAKEFEKGDLIHVFGYFKENRKGDKVYKNFIVKSMNKIEIEQMEEE
                     "
     gene            5618..5833
                     /locus_tag="AC1PNG_001551"
     CDS             5618..5833
                     /locus_tag="AC1PNG_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HIP6702048.1"
                     /translation="MDFFTQGVDVLKTLVIAIGGGLGAWGVINLMEGYGNDNPGAKSQ
                     GIKQLMAGGGIVLIGLKLIPLLANLIQ"
     gene            5842..6705
                     /locus_tag="AC1PNG_001552"
     CDS             5842..6705
                     /locus_tag="AC1PNG_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HIP6702049.1"
                     /translation="MFDIFGKLEEMIQEILLDMIKGTLTSMFTDLNHQLDFVSGQVGQ
                     SPSQFNGEVFSFIKHINDSVVLPIAGLVITAVLCLELIQVVMQKNNMAEVDTFEFFKY
                     VIKMWVAVYLVSHAFEFAMAAFDVGQHMIGQATGVIHSETSISSEQITAMVDSLKEKN
                     IGELVGIVFELQVVKLTIQIISLIILIITYGRMFEIYAYSSVAALPFATLGNRDWGQL
                     GHNYIRGLFALALQGLFLVICFGIYAVLLRGVKMTDIHMSVFSILGYTVLLGFMMLKT
                     GSLARSIMNAH"
     gene            6718..7086
                     /locus_tag="AC1PNG_001553"
     CDS             6718..7086
                     /locus_tag="AC1PNG_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HIP6702050.1"
                     /translation="MAYVQIPKDLSKVKTKIAFNLTKRQLIGFSVAGLASLPVYLSIR
                     ESVGNDLAMLVFIVLAFPFLFLTFYEKDGLTSEIYLKYIFLQQHYQPCVRYKRTLYKK
                     MHKEEVHETRKKKKTGTRVS"
BASE COUNT         2564 a         1050 c         1438 g         2109 t
ORIGIN      
        1 gatgagctag aaaaattaaa aaaacaagtt cagtatgact tattgaaaga atcaggttgt
       61 gttgagaaat tatgtgaatg ggatgaggtg ctacctatca ttgctgatgt gttttaccaa
      121 aatgaaggca caatctctat caatcaaatg agattgccga tagattttgt aaaagaacgt
      181 tttagacaat taaatatgca ccatattgac tatatctgtg agtgtttgtc aaaggtagat
      241 acaaacattc gtaatatcag agcctatatt ctaacaacag catttaatgc acctcaaaca
      301 ataggtgctt attattcaaa ccaagtaaaa caagattttg gaggaagtat attatgattt
      361 caaaaataac taaattttgc caacgtaaat taactattgg attggtttca ctttccattg
      421 gtttcattag cgtaaattct attattctta gtcctaataa tttattcaca gtttatgcta
      481 atgaagtggt atctcatcga aatgatataa ctgggagtag taaagaatgg ggtactttag
      541 ctggaaatca attcattatt gattatacaa atacgagttt tgaagtttct aaagcagcac
      601 attctagttt ttcggagaat tatcatgcta ttcgaattcc agaaacttat ccttacaaat
      661 tatttgtcaa tggacagcca aaaacattat ctgatttaaa gcgtgataaa ataggtcatc
      721 taactgctgt tatagatggc agagaagtac ctttgcttga ctacggtatg gtagagtatc
      781 agatgaatcg ctacctaggt aaggatattt ataatattta cggtaatcta tcatatactg
      841 catacgcatt tcttacaact cgtacttccc accatggata ctttgaatta actgaagcag
      901 ataacgtaag ggatgttagt ggtcagttag taactggatt ctttcaaaca cctgagtacc
      961 gtgatatggc atctgcctat cataaatcaa attcaggata cttaaaagta caagtgggtg
     1021 atcaagaact ttctacgaat attgttaacg gtcgattgaa tggactgaaa actgccaaac
     1081 aacattttta tctagtatca aatagtgaat tttcaatgga tccacaaact ggaaaaccaa
     1141 tgaaacaatt aagggaacta actgaagatg aagtacatca gttgaaagta actgaaacaa
     1201 aagtaaactc agaaatcgaa tttgacgtaa atggtaaaac atatcagaca actggacgat
     1261 taaatttttg gaaaaactcg actattaatc cattaaaaac acgacttgca gttatgtcaa
     1321 ttggtgatgg aaataatata catggttttg atttgtatgc ctcaaacaac aattacatcg
     1381 aagttgtaga aaagatagcg gaagaaccct caattcctga gtctatttct aaaatgattg
     1441 aatttgttac tgaagacggt gaaaaggtaa gtgatgggcc atctattact ggtaacaaag
     1501 gagaagaggt cacattagag attaccccac ctgatggata tacatttgtt tcaatacccc
     1561 ctgaaaggat tgtattagag gacagcaatc caattcaaat cattgttaac gagaatacag
     1621 gtcaggaaac attagaaaga gtagaagaga tagattaccg tgttattcga caagaaaatc
     1681 ctgatatgta tgttggagat gaagctattg ttagaaaagg aataagaggc acaaagacta
     1741 ttcaagatat ttttgaaaca aagaaaggta tcagaacgga taagctttta agctcaagtg
     1801 ttatttctga gactgcccca agtgatgaaa taattcacta cggcacaaaa gcgattgaag
     1861 gtgaggaaat aatcacaaga actgaaatta caccatttaa tatcatcgag gaggtagatc
     1921 caaatctata cgaaggtgaa attgtaacaa aagtcgaggg tcaaaacggt gagtttactt
     1981 attacgatta ttaccggaca attaaaggta caaaagaact cgccgctttc aaatcagaag
     2041 ctaaggaaat taagccaaaa attgataagg tcgtaagaat tgggaccaag caaattgaag
     2101 gtgagataat agaaaaatca agcgagaaga ttccatttgg aatgattatt gaagaagata
     2161 attctttatt tgaggacgaa cgtgtcgtta ttagagaagg agtccctgga atcgtagatg
     2221 tggtagcaac atataaaact agtaagggtg aaaaaatcgg agagccaatt aatgttcagt
     2281 catttattca acgtgaagca gttgatgaat tggtcaaggt tggtacaaag cctttaaata
     2341 ccacttctaa agttgagaat ttgaatgaaa ttccttttac tgttgtgaaa gagtatgatg
     2401 atagccttga agaaggtgtt gagaagataa tcaaagttgg gaaaaatgga cagaagaaaa
     2461 ttattgtgac aacaaattat gtacgtggga tacaagatgg cgaatctatc gtagaagaaa
     2521 ttacgattga agagccacag aatcaagtta tcctagtagg cacaaaaaca ttacagaaag
     2581 agtccccttc ccctaataat gataatacag acagtcagac taagatagat attccatcat
     2641 cagatgataa tcttcctaaa gtaccaaaaa taccaaataa tacgcagagt gatatagaaa
     2701 aaccaatcga gaaagaaact aaagatagtt cagaaattaa tcagaaaggt tctagtcaaa
     2761 cagatgtaaa taaaagtaaa actacaacaa aattggatag taacaccaca aataacacaa
     2821 atggttcaaa attgccagaa actggagaaa cacttgtaag tcctctgttg attatcatca
     2881 gtagtatttt ttgtttattc acttcattgg ggatgattat ttggaaggga cggaaatcaa
     2941 atgattaacg aggaggttgg caatcgtctt gtaacactaa atctaaggtt agcaaaaagt
     3001 tccgccaaaa tgataattgc agctttaaag ttagttatta aagaagctga atcagaaaaa
     3061 ttatcagtca aacaatacat atctcaaaaa agtttgcaag gggaagttcc cttaaaagat
     3121 ttagttggaa agggaacgtt agaaaatttg cctatgaaac agcctgaact tcttgaattg
     3181 aaaaaggaac taaataagca tggtgttcga ttctcggtat tgaggaataa ggagaataaa
     3241 cagtttgagg tgttttttca ggctaaagat acggctgtct tagaacattc ttttaagcag
     3301 gcagttgaac gtgcagagag aaaaacaaaa attaagcagt caactcttaa gaaaatacaa
     3361 caatatcaaa aatcacttgg ccaacatatc ataaaaccaa aaatcaaaca aaaacatcag
     3421 gaacaatcct tataggaggt ggtaggattg aagtattaaa acaaatcaaa caagatattc
     3481 ttaatgtttt cccattcgga gataggagat tactcgcaaa gagtttatta ctctatctcc
     3541 ttttttcgta tctcggtacc tgtctaggac atcatgtcaa taacttttct gatggggatt
     3601 tccttgatag gttatttcaa gcaatcctag aaattgaaac gatgcggtgg atattctttc
     3661 ctagccttga tgaaataatt actggcttaa ccttgtcagt tgttgttgga atagtacttt
     3721 attttagaaa aaagaatgca aaaaagttta ggaatggagt ggaatatggg tctgccagat
     3781 ggggtgatag gaaagatatt cagccatatg aagatccagt attcgagaat aatattcttt
     3841 tgacagatag tgagaaatta accatgaatt ctcgaccgtc gaatccaaag tttgctcgga
     3901 ataaaaatat cttggttgtc ggagggtctg gtagtggaaa gacaagattt tttgtgaagc
     3961 cgaatctaat gcaaatgcat tctagttact gtgtcacaga tccaaaagga acaatcgttc
     4021 ttgaatgtgg caaaatgtta gaaaaaaatg gctatcagat aaaaatactg aatactatta
     4081 acttcaaaaa gtcaatgaaa tataatcctt tcgcttatct aaaaagtgag aaggatattc
     4141 ttaaactagt tcaaactatt atagccaaca ccaagggaga gggagaaaaa gcgggagaag
     4201 atttttgggt caaagccgag aaactctact atacagcctt gattggttat atctactatg
     4261 aaagtccaaa agaagaacaa aactttaata ccttattggc catgattgat gctagtgaag
     4321 ttcgggaaga tgatgaaaat ttcaagaatg ccattgatta tttatttctg gccttggaaa
     4381 gaagaaaacc aaatcacttt gcggtcaagc aatacaaaaa gtataaacta gcagcaggta
     4441 aaactgccaa atctattctc atatcatgtg gtgctagatt ggcacctttt gatattcaag
     4501 agttgagaga acttatgagc gatgatgagt tggaattgga tactctggga gatagaaaaa
     4561 ctgccctatt tgtcatcatt tctgatacag atgacacctt taactttgtt gtgtccatct
     4621 tgtattctca gttatttaat ttactttgcg ataaagcaga cgatgtttat ggtggtcgtc
     4681 taccagtcca tgttcgctgt ctgctggacg aatttgcaaa cattggttta attccgagat
     4741 ttgaaaagtt aattgctaca attcgttctc gtgaaatttc tgcggcaatc atccttcaag
     4801 cgaaatctca attgaaagcc atatataagg acaatgctga cacaattatt ggaaactgtg
     4861 ataccgagtt atttcttgga ggaaaagaag gtactacgct aaaagaattg gctgaaaact
     4921 taggtaaaga aactattgaa ctctacaata caagtgaaac cagatccaat cagaaatcat
     4981 ttggtcttaa ttaccagaaa ctcggaaagg agttaatgtc cagagatgag ttaaaagtga
     5041 tggatggagg caaatgtatc ctggagattc gtgggacacg gccattttat tctgataagt
     5101 atgatattac taagcataag cattacaaac ttttagagga ttttgataag agaaacaagt
     5161 ttgatattga ggcctatctc aagaatagag gccaagccaa actgactgag aatactagta
     5221 ttatacagtt gtaggaggtg cctgatgagg attagggaac caccaatatt ctatatattt
     5281 atttagaata tcatagaaag aggaaaatta tggaacatga aatgattaat attaatgctc
     5341 atcttgtcaa agatgtcatt ttttctagct ttgaaactga ggacggtatc gtaccagttg
     5401 taaatttctc actagtaaaa aaatacggaa agggtaagga atacactaat tgttctgcct
     5461 atggcgacaa ggtagaaatt gcaaaagaat ttgaaaaagg tgacctcatt catgtgtttg
     5521 gttactttaa ggaaaatcga aaaggtgata aagtctacaa gaattttata gtaaaatcga
     5581 tgaataaaat tgaaatagaa caaatggagg aagagtaatg gattttttta cacaaggcgt
     5641 tgatgtctta aaaacgttgg taattgctat tggtggtggt ttaggtgcct ggggtgttat
     5701 caacctcatg gaaggttatg gtaacgataa ccctggagca aagtcacaag gtattaagca
     5761 actaatggct ggtggaggta ttgtgttaat tggtttaaaa ctaatcccgc tacttgctaa
     5821 cttaatccag taggaggggc tatgtttgat atttttggaa aactagaaga aatgattcaa
     5881 gaaatccttt tggatatgat aaaaggaaca ttgacaagta tgtttactga cttgaatcat
     5941 caattagatt ttgtatctgg ccaagtaggt caatcgccta gccagttcaa tggagaagtg
     6001 ttttccttta tcaaacacat taatgatagt gtcgtactac caatcgctgg attggttatt
     6061 acagctgttt tatgtttaga gttaatccaa gtagtgatgc aaaagaacaa tatggcagaa
     6121 gttgatacgt ttgaattttt caaatatgtc atcaagatgt gggttgcagt gtatttagtc
     6181 tcccatgctt ttgaatttgc gatggcagcc tttgatgtag gtcaacacat gataggacaa
     6241 gcaacaggag tcattcatag tgagacatct atttcatctg agcagattac tgccatggtt
     6301 gacagtttga aagagaagaa cattggggaa ttagtaggaa tagtctttga acttcaagta
     6361 gtaaaactaa ctattcaaat aatttcattg attattctca ttatcacata tggaaggatg
     6421 tttgagatat atgcctatag ttcagttgcc gctttgccat ttgctacact tggcaataga
     6481 gattggggtc aattgggaca taactacatt cgtgggctgt tcgcattggc tttacaagga
     6541 ttgttcctgg tcatctgttt tggaatttat gctgtattac taagaggtgt aaaaatgacg
     6601 gatattcata tgtccgtctt ttcaatttta ggttatacag tattacttgg attcatgatg
     6661 ttgaaaactg gttcccttgc aaggtcaatc atgaatgccc attagagagg agataatatg
     6721 gcatatgttc aaattccaaa ggatttatcc aaggtaaaaa caaaaatagc ttttaattta
     6781 accaagcgtc agttaattgg tttttcggtg gcaggcttag caagtctgcc tgtttattta
     6841 tctattaggg aaagtgttgg taacgatttg gctatgctcg tctttattgt actcgctttc
     6901 ccttttttat tcttaacgtt ttatgaaaag gatggattaa ctagtgaaat ctatcttaag
     6961 tatattttct tacaacaaca ctatcagcca tgtgtgagat ataagagaac actctacaaa
     7021 aagatgcaca aggaggaggt acatgagacc agaaagaaaa agaaaacagg cacaagagtt
     7081 tcttgaagca aaggaagtcc aattaaaaaa gattcaaaag gaaactctaa aacaggataa
     7141 taacacgtta cttggttcta t
//
LOCUS       DBNFEG010000116         7145 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.116, whole genome shotgun sequence.
ACCESSION   DBNFEG010000116 DBNFEG010000000
VERSION     DBNFEG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.116"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(617..1465)
                     /locus_tag="AC1PNG_001554"
     CDS             complement(617..1465)
                     /locus_tag="AC1PNG_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HIP6702051.1"
                     /translation="MTVIDGKALGVKLQAALAEKTARLKEEKGVVPGLVVILVGENPA
                     SQVYVRNKERSALAAGFKSEVVRVPDTISESDLLDLIERYNQDDEWHGILVQLPLPAH
                     ISEEKVLLAIDPDKDVDGFHPTNMGKFWSGHPVMIPSTPAGIMEMFKEYQIELEGKSA
                     LVIGRSNIVGKPMAQLLLDANATVTIAHSRTKNLPELARQADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDENGKLIGDVKYDEVSEVASYITPVPGGVGPMTITMLMEQTYEA
                     CVRSAK"
     gene            complement(1595..2329)
                     /locus_tag="AC1PNG_001555"
     CDS             complement(1595..2329)
                     /locus_tag="AC1PNG_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIP6702052.1"
                     /translation="MSNAIISIKDLHKYFGKNEVLKGIDLDIQQGQVVVIIGPSGSGK
                     STFLRTMNLLEVPTKGTVTFEGVDITDKSNDIFKMREKMGMVFQQFNLFPNMTVLDNI
                     TLSPIKTKGIAKDEAEKKAKELLEKVGLPDKANAYPQSLSGGQQQRIAIARGLAMDPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMDGGII
                     VEDGTPEEVFEHTKEERTKDFLSKVL"
     gene            complement(2322..3011)
                     /locus_tag="AC1PNG_001556"
     CDS             complement(2322..3011)
                     /locus_tag="AC1PNG_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIP6702053.1"
                     /translation="MNFSFLPEYWAYFNYGAIVTLIIAFFSVFFGSILGVLLAFAQRS
                     KYKVLAWTANIYVWIFRGTPMIVQIMIAFAMTHFVAPTFQLGILTVDLTRLIPGIIVI
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKQAMRYVIMPQAIKNILPALGNEF
                     VTIVKDSSLLSTIGVMELWNGAQTVQSTSYIPLTPLVFAASYYLIMTTVLTVIIQSFE
                     KKLNKGGQFHV"
     gene            complement(3137..3367)
                     /locus_tag="AC1PNG_001557"
     CDS             complement(3137..3367)
                     /locus_tag="AC1PNG_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HIP6702054.1"
                     /translation="MESHLVRIINRLEAMANDGGTLKRNFERDGIVVAEVAYSYDEEN
                     GSVFTLRDVAARETYAFDSIDLIAMEIYELLY"
     gene            3591..5819
                     /locus_tag="AC1PNG_001558"
     CDS             3591..5819
                     /locus_tag="AC1PNG_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIP6702055.1"
                     /translation="MLCKNCNINDATIHLYNNLNGKQQQVDLCHNCYQIMKTDPNNAI
                     LRGLGDLTNPNNMDPFSEFFNHLGGYPGNTPAGKNREQTPPTQAGEHNGRGGQTPPPQ
                     QPQQPNGLLEEFGINVTEIARRGDIDPVIGRDQEITRVIEILNRRTKNNPVLIGEPGV
                     GKTAVVEGLAQKIVDGDVPQKLRDKEVIRLDVVSLVQGTGIRGQFEERMQKLMEEIRN
                     RREIILFIDEIHEIVGAGSAGDGNMDAGNILKPALARGEMQLVGATTLNEYRIIEKDA
                     ALERRMQPVKVEEPSVEETITILKGIQNKYQDYHHVKYSDAAIEAAAVLSNRYIQDRF
                     LPDKAIDLLDEAGSKMNLTLNFIDPKEIDQRLIDAENRKAQATRDEDYEKAAYFRDQI
                     AKYKEMQKATISEEDIPLITEKEIEAIVEQKTNIPVGDLKEKEQSQLVNLASDLKAHV
                     IGQDEAVDKIAKAIRRNRVGLGAPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSADS
                     MIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEKVRRNPYSLILLDEVEKAHPDV
                     MHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGTGKVEASVGFGAAMEGRTQSVLG
                     QLSNFFTPEFMNRFDGIIEFQPLSKENLLEIVSLMLDDVNKRLSHNGISLHVTDKVKE
                     KLVDLGYDPKMGARPLRRTIQDQIEDAITDFYLEHPAEKDLRAVMSSKGTIQIKAQTK
                     TK"
     gene            6005..6466
                     /locus_tag="AC1PNG_001559"
     CDS             6005..6466
                     /locus_tag="AC1PNG_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIP6702056.1"
                     /translation="MEKIAVFGEKKAGVSYQSRFGVYAVIPDEKKEQIILVQAPNGAW
                     FLPGGEIEKGENHLIALERELMEELGFTAEIGKYFGQADEYFYSSHRDTHFYNPAYIY
                     EVTSYHQIGQPLEDFNHIAWFPVNEAIDKLKRGSHKWGIEQWKLQENSFNN"
     gene            6521..6829
                     /locus_tag="AC1PNG_001560"
     CDS             6521..6829
                     /locus_tag="AC1PNG_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HIP6702057.1"
                     /translation="MKIINTTNSHPNLVQSQLANTDAFLVETYSAGNTDVIFTQAPRH
                     YELLISNKYRAVQQNEIEQIRDFFLHRKIDERTINKAAIQTIHTDRLIEMSIPIIAEV
                     "
BASE COUNT         2277 a         1610 c         1275 g         1983 t
ORIGIN      
        1 tgaagttcat caaatcaagt caacaacgtc tgattttgat tttcgaagag tataggcaat
       61 aagtcttcat tcgcagttgt tgagctgaaa taatctgtct ccaaacgttt ttactacact
      121 gggatgttgt tcaaactttg agaaatagta ctgtttttta tacagattca tttttgcata
      181 caaaaaaacc tgatcacacg atcaggcttt tcaggagatt atgaaaaata tttaggattg
      241 ggtatagtat aaccaatatt actaaaagtc acatccgtct taagactgat ttattgaata
      301 tgaccaataa aataagtagt gtcgtttaaa aatagactga ttcatacggg gactgcactc
      361 attactgtta gctaccaaaa ctacaagcat aatttaagaa cctgtattca cagttcaacg
      421 cttctattct aaggctagtt tttaagctac tcatcattag tttttttcaa aatacaatca
      481 atatccccca aaaaaattac cttgattagc taaatctgtc tcttaatatg agtacttcgc
      541 ttgtaaatac aggttctaag aacagggctt taccaaagca ggcatggata tatttatttt
      601 tattaaactc tctatcttat tttgcgcttc tgacacaagc ttcataggtc tgttccatca
      661 acatggtaat agtcatcgga ccaacaccac ctggaactgg ggtaatataa cttgccactt
      721 ctgatacctc atcgtatttg acatcaccga ttagcttgcc attttcatca cgattcatac
      781 caacatcaat caccacggca cctggtttaa caaattcctt ggtcacaaaa tgaccacgac
      841 caatggcaac aacaagaata tcggcctgac gagcaagctc tggcaagttt ttggtgcgag
      901 aatgcgcaat cgttacagta gcattggcat ccaatagcag ctgtgccatt ggtttaccaa
      961 caatattaga acgaccaata actaaagctg acttcccttc cagctcaatc tgatattcct
     1021 tgaacatttc cataattcct gctggggtag atggaatcat aacaggatga cctgaccaga
     1081 atttccccat attagtcgga tgaaaaccat ccacatcctt atctgggtca atggccaaca
     1141 agaccttctc ctcgctgatg tgggctggta agggcaattg caccaaaata ccatgccatt
     1201 catcatcctg attatagcgc tcaatcaaat ccaacaagtc tgactcagaa atcgtatctg
     1261 gaacgcgaac aacctcactc ttgaatccag cagctagagc tgaccgctcc ttattccgta
     1321 catagacctg actagcagga ttttcgccaa ctaaaataac tacaagacct ggtaccaccc
     1381 ccttttcttc ttttaatcga gcagttttct ccgccaaagc agcctgcaat tttacaccta
     1441 gtgctttccc atcaatgact gtcataccta cactcctttt gttttttcta ttatacccta
     1501 aaattctccg actgactata cttgtacaaa gtagactata gcatatagca aaaaactccg
     1561 aacaatgtcg gagtcgtact tatttgttca tttattacaa gaccttagac aagaaatcct
     1621 tggtccgttc ttccttggta tgttcaaaga cttcttcagg cgttccatcc tccacgatga
     1681 taccgccatc cataaagata accctgtcag ctacctcacg cgcaaatccc atctcatgag
     1741 tcacgataac catggtcatc cccgacttgg ccaaatcctg cattacagca agaacttcac
     1801 caaccatttc agggtctagt gcagaggtcg gttcatcaaa aagcaggaca tctgggtcca
     1861 tggccagacc acgtgcaata gcgatccgtt gttgctgacc acctgaaagg ctttgtggat
     1921 aggcatttgc cttatctggc aatcctacct tttcaagtaa ttccttagcc ttcttctcag
     1981 cctcatcctt tgcaattccc tttgtcttaa taggtgataa agtaatgtta tctaataccg
     2041 tcatatttgg aaaaaggttg aactgttgaa aaaccattcc catcttttca cgcatcttga
     2101 aaatatcatt tgacttgtca gtaatatcaa caccttcaaa tgtaacagtt cccttggttg
     2161 gcacttctaa gaggttcatt gtacgtaaga aagtcgattt ccctgaccct gatggaccga
     2221 taataacgac aacctgacct tgttgaatat ctaaatcaat tccttttaga acctcattct
     2281 ttccgaagta cttatgtaaa tccttgatag aaataatcgc attagacatg gaactgccct
     2341 cccttgttca actttttctc aaacgactga ataatcactg ttagcaccgt tgtcataatt
     2401 agataatagc tcgctgcaaa tacaagtggt gttaaaggaa tataagaagt ggattgaact
     2461 gtctgagcac cgttccataa ttccatcaca ccgatggttg acaaaagtga actatcctta
     2521 acaatagtca cgaattcatt tcccaaggct ggaagaatat tcttgatagc ctgcggcatg
     2581 ataacgtaac gcatcgcctg ctttggtcta ataccaagcg aataagctgc ttccagctgt
     2641 ccttttggta cagcattaat acctgcacga acagtttcag atacataggc acccgagttc
     2701 atcgaaataa caatgatacc tggaatcaag cgcgtcaaat caaccgtcaa aattcctagc
     2761 tgaaaagttg gtgctacaaa atgtgtcatc gcaaaagcaa tcataatctg gacaatcatc
     2821 ggtgtcccac ggaaaatcca gacatagata ttggcagtcc aagctaaaac tttatactta
     2881 ctacgctgag caaaggcaag taacacacct aaaatactac caaaaaatac tgagaaaaat
     2941 gcaataatga gggtaacaat tgccccataa ttaaaataag cccaatactc aggcaaaaaa
     3001 gaaaaattca ttcatcttac tccaattctg tcatttagag tctattatac catttttgaa
     3061 tatttatgca atacttttgt ttataatatt caatttacac aagaaaaaag cacccttagg
     3121 tgcctctcac ttcttcttag tacaatagct catagatttc cattgcaatc aagtcgatgc
     3181 tgtcaaacgc gtacgtttca cgagcagcaa catcacgcaa ggtaaatact gaaccgtttt
     3241 cctcgtcata gctataagcc acttctgcaa caacaatacc atcacgttcg aaattacgtt
     3301 tcaacgtacc gccgtcatta gccatagctt ccagacgatt gataattctc accaaatgtg
     3361 attccatatt cgttctccta atccttttta taggaatatt ttaccacata ttgccaaaaa
     3421 agtcttattt tttccctatt ttcttcctat cgccctattt ttcatagaaa gtttttccaa
     3481 gcgtagcagt ttcttgattt ttctatgaat cttgttataa taaagctaga actgaccttt
     3541 tttgactatt taacaatagg tcacattttt tcacagaaag aggtagaatt atgctctgca
     3601 aaaattgtaa tatcaacgac gcaacgatcc atctctacaa caacttaaac ggtaaacagc
     3661 agcaggtaga cctctgtcac aactgctacc aaatcatgaa aacagaccct aataatgcca
     3721 tcctacgtgg gttgggagat ttaacaaatc ctaacaacat ggatcccttt agtgaatttt
     3781 tcaatcacct aggtggctat cctggcaata cccctgctgg aaaaaatcgc gaacagactc
     3841 ctcctactca agccggtgag cataatggac gtggtggaca gacccctcct ccgcaacaac
     3901 cccaacaacc aaatgggctc cttgaagaat ttggtatcaa cgttaccgaa attgcccgtc
     3961 gtggggatat tgatccagta atcggtcgcg atcaagaaat tacccgtgtc atcgaaattc
     4021 tcaaccgtcg tacaaaaaac aaccctgttc ttatcggtga gcctggtgta ggtaaaacag
     4081 cggttgttga aggcttggct caaaaaattg tcgatggcga tgtcccacag aaattacgag
     4141 acaaggaagt cattcgtctg gatgttgtca gcctcgtaca aggaacaggt atccgtggac
     4201 aatttgaaga acgcatgcaa aaactcatgg aagaaatccg taatcgtcgc gaaatcattc
     4261 tttttattga cgagattcac gaaattgtcg gtgctggctc tgcaggcgat ggtaacatgg
     4321 atgcaggcaa tatcctcaaa ccagccctcg cacgtggtga aatgcaacta gtcggtgcaa
     4381 caacgctcaa cgagtaccgt attatcgaaa aagatgccgc cctagaacgc cgtatgcaac
     4441 ctgtaaaagt tgaagaacca agtgtcgaag aaaccattac cattctcaaa ggtatccaaa
     4501 ataaatacca ggattaccat catgttaaat actccgatgc tgctatcgaa gctgccgcag
     4561 tactgtccaa ccgctacatt caagaccgtt tcctaccaga caaagctatt gaccttctgg
     4621 atgaagctgg ttcaaaaatg aatttaaccc tcaactttat tgatccaaag gaaattgacc
     4681 aacgcctgat tgatgccgaa aatcggaaag cacaagctac acgggatgag gactatgaaa
     4741 aagcggccta tttccgtgac caaattgcta agtacaagga gatgcaaaag gcaactatca
     4801 gcgaggaaga tattccactc attactgaaa aagaaattga ggccatcgtt gagcaaaaaa
     4861 caaatatccc agttggtgat ttaaaagaaa aggaacagtc ccagcttgtg aacctcgcta
     4921 gtgacttaaa agcccatgtt atcggacaag atgaagcagt tgacaagata gctaaagcca
     4981 tccgccgtaa tcgtgtcggt ctcggtgctc caaatcgtcc cattggatcc ttcctctttg
     5041 tcggaccaac aggtgtcggt aaaaccgaat tgtctaaaca attagctatc gaactcttcg
     5101 gttcggcaga cagcatgatc cgcttcgaca tgtccgaata catggaaaaa cacgctgtcg
     5161 ctaaactcgt cggagctcct ccaggctatg taggctatga ggaggctgga caattgacag
     5221 aaaaagtccg tcgtaatcct tactcactca tcctccttga cgaagttgaa aaagcccacc
     5281 cagatgtcat gcacatgttc cttcaggtct tagacgatgg tcgtctgaca gatggacaag
     5341 gtcggacagt cagcttcaaa gatactatta tcatcatgac atcaaatgct ggtactggaa
     5401 aagttgaagc aagtgtcggt tttggtgcgg ctatggaagg gcggactcaa tcagtccttg
     5461 gccaacttag caatttcttc acacctgaat ttatgaaccg atttgacggt attattgagt
     5521 tccaaccact gagcaaggaa aacctacttg aaatcgtcag cctcatgcta gacgatgtaa
     5581 acaaacgcct ttctcacaat ggcatcagcc tccatgtaac agataaagtt aaggagaaat
     5641 tagtggacct tggctacgat ccaaagatgg gagctcgtcc actgcgcaga accatccaag
     5701 atcagattga agatgctatc accgacttct acttagaaca tccagcagaa aaagacctac
     5761 gtgctgtcat gtcaagtaaa ggtacaattc aaatcaaggc acaaacaaag acaaaataag
     5821 aaaatagagc tgctccctac gagtagccta ttttactgtt ttgctactct atttttctaa
     5881 attcttcccc atctccatcc tataaaaaaa ctttactttg taaagcctat cacaaggaaa
     5941 aataataaaa aagtggtata atgtatacaa ctcattccaa taatgaaaga ggatagaatt
     6001 gcctatggag aaaattgctg tatttggaga aaagaaggct ggcgtcagct accagagtcg
     6061 ctttggtgtt tacgcagtta tcccagatga aaaaaaagaa cagattatac tcgttcaagc
     6121 accaaacgga gcttggtttc ttcctggtgg agaaattgaa aagggagaaa atcaccttat
     6181 tgctttggag cgcgaattga tggaagagct tggattcacc gctgaaatcg gaaaatactt
     6241 tggtcaggct gacgaatact tctactctag ccaccgcgat acccatttct ataatccagc
     6301 ctacatctat gaagtgacta gctaccatca aataggacaa ccactagaag atttcaacca
     6361 tattgcttgg ttcccagtca atgaagccat cgataaactc aaacgtggta gtcacaaatg
     6421 gggcattgaa caatggaaat tgcaagaaaa ttcattcaat aactagtaaa aacactctaa
     6481 tcgtgctata atgtagccaa agagatagag gaggttccac atgaaaatca ttaacacgac
     6541 aaatagtcat cccaatcttg tccaaagcca gttggctaat actgatgctt tcctagtaga
     6601 gacttattct gcaggtaata cagacgttat tttcacacag gcaccccgtc attatgaact
     6661 cttgattagt aataaatacc gtgcagtaca acaaaatgaa atcgagcaaa ttcgtgattt
     6721 cttccttcat cggaaaattg atgaacgaac tattaacaag gctgccattc aaacgattca
     6781 cacagaccgt ttgattgaaa tgtctattcc cattatcgca gaagtgtaag aagccagatg
     6841 gcttctttca gattgaagaa aaagtccatt ttggacaatt ttcttcaatc ttttttcttt
     6901 atattgaaaa gaaaaactct cagaaacgct gaaatatcaa tgtttccaag agtttaatca
     6961 cttgctatct ttcgcaaacc tcttcaattt tttatatttt taggagtttg tctacgttct
     7021 gtaagaagcc aattggcttc tttttttcta tttatactca atgaaaatca aaagtagcct
     7081 aggaaacgaa gccgaagata gaactggagt tcatcaaggc tcttgacaac gaataatttt
     7141 gattt
//
LOCUS       DBNFEG010000117         6966 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.117, whole genome shotgun sequence.
ACCESSION   DBNFEG010000117 DBNFEG010000000
VERSION     DBNFEG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6966
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.117"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..510
                     /locus_tag="AC1PNG_001561"
     CDS             <1..510
                     /locus_tag="AC1PNG_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702058.1"
                     /translation="LLTFLFYCQIEILLVIFSTMILGEFLSIFIYSVVFMIALLERYQ
                     WFSKTSYNSLYGNVDFSNPLERYVELFVAHSRKFGWLVLTPVFLYRIFFSGGENLYRN
                     DLIRTVSILSFPIIGLVGLYFVIALGASQIKGYYISKYMEDYRILSGYSIEDWYGKKS
                     RRYKESLGK"
     gene            599..1459
                     /locus_tag="AC1PNG_001562"
     CDS             599..1459
                     /locus_tag="AC1PNG_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004229812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702059.1"
                     /translation="MKKTIWNASLEESMNLVTDKYIKTSMDDVNQYGHKKKILGFFTM
                     VLFIGLLSLIGSYIDKESGVLLFLKAELLFRIPLVLSLLFLFCYLMELRKVKEYRVLS
                     YYIFNRYMFLVMSCFILQFWLICAVGFAILWGSVLSIIVNVGIFSIVVSERLISFVKK
                     TEGVLYQGQPSNNILYKFLEKFVAFSKRYGGGLVVLLLVSRFVFKNSFQSASGEGIYH
                     NDFLRSLAMTFSVFFPLLPFYFSVAIATSCIEGYYLEKYSEDYRQLSGYSVEDWYGKK
                     SRRYKESLGK"
     gene            complement(1672..2541)
                     /locus_tag="AC1PNG_001563"
     CDS             complement(1672..2541)
                     /locus_tag="AC1PNG_001563"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIP6702060.1"
                     /translation="MKIDIRIPQAFPQLTVKEVLEDYFLIPRKIRHFLRTKKHIRVNG
                     ELINWQSPITAGDLLELTFDQEDYPEKSIPLGQADLVEELYQDEHLIIVNKPEGMKTH
                     GNEPTEIALLNHVSAYVGQTCYVVHRLDMETSGAILFAKNPFILPILNRLLEDKVIYR
                     DYLALCQGQLKKTDWIITDKIGRDRHDRRKRVVDNRKGQVALTQVRLLKTLSKNSLVT
                     CRLQTGRTHQIRVHLAHHDHALIGDPLYSQVAAPRLMLHAQKLSLTHPLTLEEIRVEA
                     RSESFEKVLKNMK"
     gene            complement(2543..3511)
                     /locus_tag="AC1PNG_001564"
     CDS             complement(2543..3511)
                     /locus_tag="AC1PNG_001564"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIP6702061.1"
                     /translation="MTQVYDITIIGGGPVGLFAAFYAHLRQAKVKIIDSLPQLGGQPA
                     ILYPEKAILDIPAFPSLTGQELTDNLLAQLAPFDTTICLNETLTAIEPGEVISLTTNK
                     GSHQTKTLIIAMGGGAFKPRPLEIDGADSFDNVHYHVSNIQQYADKDIVVLGGGDSAV
                     DWSLAFEKIAKTTQIVHRRDNFRALEHSVEELKQSSVTIHTPFIPKGLSGENGRASAI
                     DFDKVKSEDKLTLSFDHLFVNYGFKSSVGTLKEWGLELNRHRIVVNSKQETSVPGIYA
                     IGDCCFYEGKVDLIATGLGEAPTAVNNAMNYLNPNEKVQPKHSTSL"
     gene            3664..5877
                     /gene="pbp2a"
                     /locus_tag="AC1PNG_001565"
     CDS             3664..5877
                     /gene="pbp2a"
                     /locus_tag="AC1PNG_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2A"
                     /protein_id="HIP6702062.1"
                     /translation="MDDLQHQPIEVPEDFQIDHEDRPSRRRRGNRGSVDKPKKKSRIP
                     EPIRKFWRRYQLTKILIILIVLTVLTVGGYLFFLAKTANVGDLQQALKATTIIYDKDG
                     AEAGTLSGQKGTYVELDAVSDNLENAVIATEDRSFYKNSGINYQRTILAALTLGRSGG
                     GSTITQQLAKNAFLTQDQTISRKAREFFLALEINKKYSKQEILTMYLNNAYFGNGVWG
                     VEDASQKYFGIPASDLTLEQAAVIAGMLKGPEIYNPYYSLENAVNRRDTVLQNMVNAG
                     YIDQATADAGFQVDLASQLSDTYTGKQSNYSYPSYFDAVIAEAIERYGLKESDIINNG
                     YRIYTEMDQNSQASMQVIFNDETMFPTSSFDGTHAQAASVALDPRTGGVRALVGRVNS
                     SEDAVFRTFNFATQAKRSPGSTIKPLVAYAPAVAAGWSIDMALDNHTETYGDYTLHNY
                     DYSTSDTIPMYQALALSYNLPVAYIVNTLGIDKAFEYGQKFGLDMENVEKVLGVSIGS
                     GVETNPLQMAQAYATFANKGVMKDAHLITRIETASGKVLAEHRETSTKVIDRSVADKM
                     TAMMLGTYTNGTGVTADTANYHIAGKTGTTETSFDVNLVNDQWHIAYTPDLVISQWVG
                     FEQTDENHYIDSSSYWKAPSVFQTVTNSILPYTAGTKFEVENAYAQNGIVEVAPDEAA
                     QTESSLGQEQVQEISEQAKNLIEQTKQNLIDAKLPERAKTLWDNITSWFGELSNP"
     gene            5911..6063
                     /gene="rpmG"
                     /locus_tag="AC1PNG_001566"
     CDS             5911..6063
                     /gene="rpmG"
                     /locus_tag="AC1PNG_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIP6702063.1"
                     /translation="MALKKASLACTVCGSRNYSISLSSNPKPTRLEVNKFCKHCGQYT
                     VHKETR"
     gene            6073..6249
                     /gene="secE"
                     /locus_tag="AC1PNG_001567"
     CDS             6073..6249
                     /gene="secE"
                     /locus_tag="AC1PNG_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIP6702064.1"
                     /translation="MKFIKDIIRILKDTSWPTRKQSWTDFVSVVEYTAFFVAVVYLFD
                     LILSRGIMSLINLF"
     gene            6359..6898
                     /gene="nusG"
                     /locus_tag="AC1PNG_001568"
     CDS             6359..6898
                     /gene="nusG"
                     /locus_tag="AC1PNG_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIP6702065.1"
                     /translation="MYDSFDKGWFVLQTYSGYENKVKENLLQRAHTYNMLENILRVEI
                     PTQTVQVEKNGEVKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRQILVSMGQTVQEFDIDVKVGDTVRIIDGAFTDYTGKITEIDNNKVKMVI
                     SMFGNDTIAEVNLSQIAEL"
BASE COUNT         1957 a         1282 c         1640 g         2087 t
ORIGIN      
        1 ttgcttacat ttctattcta ttgccaaata gaaatattac tagttatttt ttcgacaatg
       61 atacttggtg agttcttgtc aatattcatc tattcggtgg ttttcatgat tgcccttctt
      121 gaaagatatc aatggtttag taagacttcg tacaatagtt tgtatggtaa tgtagatttc
      181 agtaacccac tagaaagata tgtggagctt tttgttgctc attctagaaa atttggttgg
      241 ctcgttctta ctccagtttt tctgtatagg atattttttt ccggcggaga aaatttgtat
      301 agaaatgatt taataagaac ggttagcatc ctttcgtttc ctattattgg attggttggt
      361 ttatattttg ttattgcact tggtgcaagt cagataaaag gctattatat cagtaaatat
      421 atggaagatt atcgtattct ctcaggatat agcattgaag actggtatgg taaaaagtct
      481 aggcgatata aggaaagttt gggaaagtga taaatacaag caggaaatat taaaattagt
      541 agcaaaaagt gtatttaact aagagctaag actatttgct attggttaag gagaaaacat
      601 gaaaaaaaca atatggaatg cttctttaga agagagtatg aatcttgtaa cagataaata
      661 catcaaaact tctatggatg atgtgaatca atatggacat aagaagaaaa ttttaggttt
      721 ttttacgatg gttcttttca tcgggctact cagtttaatt ggaagctata ttgataagga
      781 atctggagta ttgttgtttt taaaagctga actattattt cgaatcccat tagtactaag
      841 tttgttgttt ttattctgtt atctgatgga attgagaaaa gtcaaggagt atagagttct
      901 atcctactat atttttaata ggtatatgtt tttggtgatg agttgtttta ttttacaatt
      961 ttggctgata tgtgctgtag gatttgcgat tctatggggt tcagttctgt caattattgt
     1021 caatgtcggt atttttagta tagtggtatc agaacgtctt atttcgtttg taaagaaaac
     1081 agaaggagta ttgtatcaag gacaaccatc aaataacatc ttgtacaaat tcctggaaaa
     1141 atttgttgct ttttcaaaaa gatatggtgg tggtttagtt gttcttctcc ttgttagtcg
     1201 ttttgtattt aaaaatagct ttcaaagtgc aagtggagaa ggcatttacc ataatgattt
     1261 tttacgctcc ctggcgatga catttagtgt ttttttcccc ctcctcccgt tttatttctc
     1321 tgtagctatt gctactagtt gtatagaggg atattacttg gagaaatatt ccgaagatta
     1381 ccgtcaacta tctggttatt ccgtagaaga ctggtatggt aaaaagtcta ggcgatataa
     1441 ggaaagtttg ggaaagtagg ttttgtttag atcatattag tttacttttg gtttatggct
     1501 aagcaatgta ttccaaaatc ccaagttgca taattttttg atgcttgagc tattaacttg
     1561 taaacccaga caaaattacc ttgtctggat tttttggtta gtggaagcat aaaaaaattc
     1621 actggattag tccagtgaaa tagggatata gcactctaag ggtgcagcta tttatttcat
     1681 gtttttcaag actttctcaa aactctccga ccgtgcttcc acgcggattt cttccaaggt
     1741 cagcgggtgg gtcaggctga gtttttgggc gtggagcata aggcgaggtg ctgcgacttg
     1801 actgtagagc ggatcaccaa tcagggcgtg atcgtggtgg gctagatgga cccggatttg
     1861 atgggttcgg cctgtctgaa ggcggcaggt tactagacta tttttgctaa gagtcttcaa
     1921 gaggcggacc tgggtcagtg ccacctgtcc cttgcgatta tcgaccaccc gctttctacg
     1981 gtcgtgccgg tcccgtccga ttttatcggt gatgatccaa tctgtctttt ttagctggcc
     2041 ttggcagaga gctagatagt cacggtagat gaccttgtcc tccaaaagac gattgagaat
     2101 gggtaggata aagggatttt tagcaaagag aatggctccg ctggtttcca tatctagcct
     2161 gtggactaca tagcaggtct gaccgacata ggcggataca tggttgagca gggcaatttc
     2221 tgtcggctca ttgccgtggg ttttcatgcc ttcaggcttg ttgacgataa tcaaatgctc
     2281 atcctgatag agttcctcca ccaagtccgc ctgtcccaga ggaatacttt tttcaggata
     2341 atcttcctgg tcaaaggtca attccagcag gtcgcctgcg gtaatcgggc tctgccagtt
     2401 aatcagctca ccgttgacac ggatgtgctt cttggtgcgg aggaagtggc ggatttttct
     2461 gggaatgaga aaataatctt ccaagacctc tttgacagtc agttgtggaa aggcttgggg
     2521 aatacgaatg tcaattttca tcttataggc tggtcgagtg ctttggttgc actttttcgt
     2581 ttggattgag gtagttcatg gcattgttaa cagcggttgg ggcttcgccc agtccggtcg
     2641 caatcaggtc aactttgcct tcatagaaac aacagtcacc gatagcatag atgccaggga
     2701 cagaggtttc ttgcttgctg ttgaccacga tgcggtggcg attgagctcc aatccccatt
     2761 ctttcagcgt gccgacagat gatttgaaac cgtagttgac aaagaggtgg tcaaaagaca
     2821 gggtcagctt gtcctcgctt tttaccttat cgaaatcaat ggcagaagct ctgccatttt
     2881 ccccagaaag ccctttaggg ataaaaggtg tgtgaatagt aacgctggac tgcttgagct
     2941 cctccacgct gtgctcgagg gctctgaagt tatcgcgtcg gtggacaatc tgggtggtct
     3001 tggcgatttt ttcaaaggcc agggaccaat cgaccgcaga gtcaccgcca cccaatacga
     3061 cgatgtcctt gtcggcatac tgctggatat tagacacgtg gtagtggacg ttgtcaaagc
     3121 tgtcagcacc atcaatctcc agcggacgtg gcttgaaggc cccgccaccc atggcgataa
     3181 tcagggtctt ggtttggtga ctgcctttat tggtcgtcag gctaatgacc tccccaggct
     3241 caatggcagt cagggtttcg ttgaggcaga tggtggtgtc aaatggagcc agttgagcga
     3301 ggaggttgtc ggtcaattcc tgaccggtta ggcttggaaa ggctggaata tccaaaatgg
     3361 ccttctcagg atagagaatg gcaggctggc cacccagttg gggtagggag tcaatgattt
     3421 tgaccttggc ttgacggaga tgggcgtaga aggcagcgaa gagaccgaca ggcccgccac
     3481 cgataatggt aatgtcatat acttgtgtca tgggaatcct cttctttgtt cagaatgacc
     3541 ttattttatc atatttttgg aaaattttct ctgtctgttg tcacaagtgg tcgcaggctt
     3601 ttttctgcta gtttttagga aatggtgtat aatggtacaa aattcactag aaagaggtag
     3661 agtatggacg atttgcaaca tcagccgatt gaagttccag aagatttcca aattgatcat
     3721 gaagaccgac ctagccgtcg ccgcagggga aatcgtggct ctgttgataa accaaagaaa
     3781 aaatcacgta ttccggagcc gattcgtaaa ttttggcgcc gttatcaact gactaaaatt
     3841 ttaatcattt taatagtcct gacagttctt acagtaggtg gctatctatt tttccttgca
     3901 aagacagcta atgttggtga tttacagcag gcgctaaaag ccacaacgat tatttacgat
     3961 aaagatggtg cggaagctgg gacattgtcg ggtcagaagg ggacctatgt agagctggat
     4021 gccgtctctg ataatttaga gaatgccgtt atcgctacag aagatcggag cttctacaaa
     4081 aattcaggca ttaattacca gcgtacaatt ttggctgcct tgacgttagg tcggtctgga
     4141 ggtggttcaa ctattaccca gcaattggca aaaaatgcct ttctaactca ggatcagacc
     4201 atcagtcgaa aggctcgaga attctttttg gctctggaaa tcaataaaaa gtacagcaag
     4261 caagaaattt tgaccatgta tctcaacaat gcctactttg gaaacggtgt ttggggagtg
     4321 gaagatgcaa gtcaaaagta cttcggtatt ccagccagtg atcttactct tgagcaagcg
     4381 gccgtcatag caggtatgct caagggacct gagatttata atccttatta ttctctagaa
     4441 aatgcagtta atcgtcggga tacagtcctg caaaacatgg tcaatgctgg ttatattgat
     4501 caagcgactg cagatgcagg tttccaagta gacctagcca gtcagctttc agatacttat
     4561 acagggaaac aaagcaatta ttcctatcca tcctattttg atgctgtgat tgctgaggcg
     4621 attgagcgat acggactgaa agaatcagac attattaata acggctatag gatttacaca
     4681 gagatggatc aaaattctca agccagcatg caggtgattt ttaatgatga gactatgttc
     4741 ccaacatcaa gttttgatgg tacccatgct caggctgcca gcgtagctct tgatcctagg
     4801 actggtggag ttcgcgcctt ggttggtcga gttaacagtt cagaggatgc ggtctttcgg
     4861 accttcaact ttgcgacaca agccaagcgc agtcctggtt caacaatcaa gcctcttgtt
     4921 gcctatgctc cagcagtagc ggcgggctgg tcgattgata tggctctgga taaccataca
     4981 gaaacctacg gcgattacac cttacataac tatgattatt cgacttctga taccattcca
     5041 atgtatcaag ctttggctct ctcttacaat ttaccagtag cttatatagt gaacaccttg
     5101 ggtattgaca aagccttcga gtatggacaa aagtttggac ttgatatgga aaatgtcgag
     5161 aaagttttgg gtgtttcaat tggttccgga gtggagacca atcctctgca aatggcccag
     5221 gcctacgcta cttttgcgaa taagggtgtg atgaaggatg cccacctcat cactcgtatt
     5281 gaaacggcta gtggaaaggt tttagcagaa catcgagaaa caagtaccaa ggtgattgac
     5341 cgctctgtcg cagataagat gacagcaatg atgcttggta cctataccaa tggtacggga
     5401 gtgacagcag atacggccaa ttatcatatc gctgggaaaa caggaacaac cgaaacgagt
     5461 tttgatgtca atctcgtcaa tgaccagtgg catattgcct acacacctga tttggtcatc
     5521 agtcagtggg ttggttttga gcagacagat gaaaatcatt atatagatag cagtagctac
     5581 tggaaggctc cgtcggtctt ccagactgta accaattcaa tcttgccata tactgcagga
     5641 actaagttcg aagtagaaaa cgcctatgca caaaatggta ttgtagaagt agctccagat
     5701 gaagctgcgc aaactgaatc aagccttggt caagaacagg tacaagaaat ttcagaacag
     5761 gcgaaaaact tgattgagca aaccaaacaa aatctaatcg atgccaagtt gccagaacga
     5821 gcaaaaacct tgtgggataa cattacgtct tggtttggag agttgtcaaa tccataaaaa
     5881 catggtagaa tgaaaaggat ggagggcttt atggcactaa aaaaagcaag cctagcctgt
     5941 actgtttgtg gttctcgaaa ctattcaatc agcctttcga gcaatcccaa gccaacacgg
     6001 ttagaagtaa ataaattttg taaacactgt ggacaataca ctgttcacaa ggaaaccaga
     6061 taggagtcac gcatgaaatt tatcaaagac attatccgca ttttaaaaga tacgtcttgg
     6121 ccaactcgta agcagagctg gacggatttc gtgtctgtag tggaatatac tgcctttttc
     6181 gtagcggtgg tttacctctt tgacttgatt ttgtcacgag gaatcatgag cttgatcaat
     6241 cttttctaga aaatacttgc catttgtaaa atggaagtga tataataagg aaaagcgaaa
     6301 gccttcgggc tttttttagc gtctaaaaat tccgtgtttc caaagaaagg aagtaacaat
     6361 gtacgatagt tttgataaag gctggtttgt tttacagacc tactcaggat atgaaaataa
     6421 agtgaaggaa aacttgctcc aacgtgctca cacttataac atgttggaaa acatccttcg
     6481 tgtagaaatt ccaactcaga ctgttcaggt tgaaaagaac ggtgaggtca aggaagtgga
     6541 agaaaaccgt ttccctggtt atgttttggt tgagatggtt atgaccgatg aagcttggtt
     6601 tgtggttcgt aatacgccaa atgttacagg tttcgtgggt tcacacggta accgttcaaa
     6661 accaactcca cttttggaag aagaaattcg tcaaatcttg gtgtctatgg gacaaaccgt
     6721 tcaagaattt gatattgatg tcaaagttgg cgacactgtt cgtatcatcg acggtgcctt
     6781 cacagactac acaggtaaaa ttacagaaat cgacaacaac aaggttaaaa tggttatctc
     6841 tatgtttggt aatgatacca ttgctgaagt taatttgagc cagattgctg agttgtaata
     6901 agttgttaaa gcgcagggaa gcctgtgctt tttcttgttt ttgagggcta attcaaaaaa
     6961 aacttg
//
LOCUS       DBNFEG010000118         6879 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.118, whole genome shotgun sequence.
ACCESSION   DBNFEG010000118 DBNFEG010000000
VERSION     DBNFEG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.118"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(56..259)
                     /locus_tag="AC1PNG_001569"
     CDS             complement(56..259)
                     /locus_tag="AC1PNG_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HIP6702066.1"
                     /translation="MIEIHCLDAYKQERIQTFENKDATILAFSGCLTLPDYYPVTSIT
                     QDGQALDYKGTIGDLYRYLQTLD"
     gene            complement(261..605)
                     /locus_tag="AC1PNG_001570"
     CDS             complement(261..605)
                     /locus_tag="AC1PNG_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HIP6702067.1"
                     /translation="MAFQQSVTLKDCNFTYHISPAIKKYTLRDNTFEQTKAGHYQLTR
                     LLEEIPNSNQGFLLKIIINQDLTGFKINITDKSGLHLVNIFKPESNKVIQEKFYFLMD
                     SLIDRDIFTKES"
     gene            complement(607..1722)
                     /locus_tag="AC1PNG_001571"
     CDS             complement(607..1722)
                     /locus_tag="AC1PNG_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIP6702068.1"
                     /translation="MIYLDNAATTALSPTALQRMMEVAQENYGNPSSIHQSGRKANQR
                     LRQSRQEIAQILSASPEQIIFTSGGSEADTLAIQGYALAHQSKGKHLITTAIEHHAVL
                     HTMQYLEERFGFEVTYIQPVDQVITAQQIQDALRPDTILVSVMFANNETGQLLPIKEI
                     GELLADHQAVFHVDAVQAIGKIAISPSNYGIDLLAASAHKFHGPKGTGFLYSKVHKFD
                     SLIHGGKQEQQHRAGTENLPAIAAMATALTEQYESLDTHHQHVSELRQHIIEGLSGLD
                     YYLNQAGPHLPHVLNIGFPGKLNEQLLMQLDLAGIAVSSGSACTAGVVQNSHVLEAMY
                     GKDSHRLKESIRISLSEINTKEEIDTLIIELKKILGG"
     gene            complement(1733..2710)
                     /locus_tag="AC1PNG_001572"
     CDS             complement(1733..2710)
                     /locus_tag="AC1PNG_001572"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIP6702069.1"
                     /translation="MVQPLGEHNIKLFSLNSNRDIAEKIADSAGIPLGKLSSRQFSDG
                     EIQINIEESVRGVDVYIIQSTSYPVNNHLWELLIMIDACKRASANTITAVIPYFGYAR
                     QDRTASPREPITAKLVANMLVKAGVDRVLTLDLHASQIQGFFDIPVDNLLTEPLFAAY
                     YMEKGLCGEDVVIVSPKNSGIKRARNIAEFLNAPIAIIDYAQDDSERSEGYIIGDVAG
                     KKAILVDDILNTGRTFSQASKIVQEGGATEIYAVASHGLFAGTATQLLDEAPIKEILV
                     TDSVASKEQHPKNIVFLTASDLIADAIHRIQEHQPLSPLFKFTSPEKEN"
     gene            complement(2855..3421)
                     /locus_tag="AC1PNG_001573"
     CDS             complement(2855..3421)
                     /locus_tag="AC1PNG_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HIP6702070.1"
                     /translation="MKNHLEIEYKTLLTKSEYKRLLTDFSDVSPILQTNHYIDTPDLD
                     MKNHRFSLRIRTFEDSAELTLKIPQEVGNQEYNQVLDIQTAHDLLENFQLPAGQISDI
                     ISATEIPLDKLAVWGILTTKRYEKQTSIGLMALDENDYVGQKDYELEVEVTDADAGKI
                     LFDEFLKKKSIQFKYASSKVARTAAHMK"
     gene            3508..4179
                     /locus_tag="AC1PNG_001574"
     CDS             3508..4179
                     /locus_tag="AC1PNG_001574"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HIP6702071.1"
                     /translation="MDFNWEEFLDPYIQTVGELKIKLRGIRKQYRKANRHSPIEFVTG
                     RVKPIESIKEKMALRHIKLENLAQDMQDIAGLRIMVQFVDDIEEVLAILRKRKDMQIV
                     HERDYINNMKASGYRSYHVVIEYPVDTINGNETVLAEIQIRTLSMNFWATIEHSLNYK
                     YKGNFPEEIKKRLEVTAKIAYELDEEMRKIRNDIQEAQALFDPAYRKLNDGVGNSDDT
                     DEEYR"
     gene            4163..4981
                     /locus_tag="AC1PNG_001575"
     CDS             4163..4981
                     /locus_tag="AC1PNG_001575"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HIP6702072.1"
                     /translation="MKNTGRKKIALLASRNPKSEAVSKELWTKLKEANFILTPKNPDI
                     VISIGGDGMLLSAFHKYEKLIDRVRFVGIHTGHLGFYTDYRDFEVDKLIENLKLDTGA
                     RVSYPILNVKVKMTDGRIVEARALNEATVKRLSKTMVADIIINNVPFERFRGDGISVS
                     TPTGSTAYNKSLGGAVLHPTIEALQIAEVASLNNRVYRTLGSSVVVPKKDKIVIEPKH
                     SDRYSIAVDNKTFVYDSIESIEYQIDNSKIHFVATPSHTSFWNRVKDAFIGEVE"
     gene            4981..5874
                     /locus_tag="AC1PNG_001576"
     CDS             4981..5874
                     /locus_tag="AC1PNG_001576"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIP6702073.1"
                     /translation="MRFEFIADQHTKIKTFLKKHGVSKGLLAKIKYTGGNIWVNDIEQ
                     NATYLLDIGDRVTIDIPAEEDLTGSLKPIFFPLDIVYEDDHFLSINKPVGYASIPSAL
                     HSSTIANFVKGYLIEQNYENKQVHIVTRLDRDTSGVMLFAKHGYAHARLDKQLQAKLI
                     HKRYYALVKGNGQLEETGDIIAPIGRPEDSIITRCVTKNGKYAHTSYRVVQSWGDIHL
                     VDIQLHTGRTHQIRVHFSHIGFPLLGDDMYGGSLECGIERQALHCHNLAFDNPFSAER
                     IDLEAPLPEDFQAVINQLISK"
     gene            5892..6866
                     /gene="pta"
                     /locus_tag="AC1PNG_001577"
     CDS             5892..6866
                     /gene="pta"
                     /locus_tag="AC1PNG_001577"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HIP6702074.1"
                     /translation="MKIFEQLATKLEGKKVRIVLPEGEEPRILQATKRLVNESDVVPV
                     LLGNPDRIKIYLDIEGITEGFEIIDPAHYDRYDEMVAALVERRKGKVSEEQAHELLKD
                     VNYFGVMLVYMGVVEGMVSGAIHSTASTVRPALQIIKTEPGVSKTSGAFLMVKGEERY
                     IFGDCAINIDPDAQTLAEIAINSARTAKMFGIDPKVAMLSYSTKGSGAGPKVDKVVEA
                     TKIAQKLRPELDLDGELQFDAAFVPTTGKLKAPGSSVAGQATVFIFPGISAGNIGYKI
                     AERMGGFAAVGPVLQGLNQPVNDLSRGCNPDDVYNLTLITAVQALEHR"
BASE COUNT         2003 a         1191 c         1560 g         2125 t
ORIGIN      
        1 agtttgtgaa aaaatcaaca gattgatttt aaaaaaagat taaacaggaa actgtctaat
       61 ctagtgtttg taaatagcga tagagatctc cgattgtccc tttgtaatcc agagcttgtc
      121 cgtcttgtgt aatgctggta actggataat agtctgggag ggttaggcaa ccggaaaaag
      181 ccagaatggt tgcatcctta ttctcaaatg tttggatacg ttcttgcttg taagcgtcaa
      241 gacagtgaat ttcaatcata ctaactctct tttgtaaaga tgtcacggtc aatcaagcta
      301 tccatgagga aatagaattt ttcttgaata accttgttac tttctggctt aaaaatgttt
      361 accagatgaa gaccagactt gtcggtaatg ttaattttaa aaccagttag gtcttgattg
      421 ataataattt tcaagaggaa tccttggttt gaatttggaa tctcttccag aagacgtgta
      481 agctgatagt gacctgcttt ggtttgttca aaggtgttat cacggagtgt atattttttg
      541 atagcagggc taatgtgata agtaaaatta caatctttta aggtaactga ttgttggaat
      601 gccattttaa cctccaagta ttttctttag ttcgatgata agagtatcta tctcttcttt
      661 tgtattgatt tcagataggc tgatacgaat ggattctttc agacgatggg agtcttttcc
      721 atacatagct tctaaaacat ggctattttg gacaacacct gccgtacatg cagaaccact
      781 agaaacagcg atacctgcta aatctagttg cataaggagt tgttcattaa gtttaccagg
      841 aaaaccgata ttaaggacat gagggagatg aggtccagcc tgattgagat aatagtctaa
      901 gccagacaat ccctcgataa tatgttgtcg cagttcactg acatgttgat gatgcgtatc
      961 taaggactca tattgttctg ttagggcagt cgccatagca gcaatagcag ggagattctc
     1021 agtacctgct cgatgttgct gttcctgctt tccgccgtgg atcaaggaat caaatttatg
     1081 aactttacta tacaggaatc ccgttccctt tggaccatgg aatttatggg cagaggccgc
     1141 gagcaagtca attccgtagt tagatggaga tattgcgatt tttccaattg cttggacagc
     1201 atctacatga aagacggcct gatgatcggc taggagttct ccaatttcct tgatgggaag
     1261 tagctgaccg gtctcattgt tagcaaacat gacgctgact aaaatagtat ctggccgaag
     1321 ggcatcctga atttgttgag cggtgattac ttggtcgact ggttggatat aggttacctc
     1381 gaacccaaac cgttcctcta gatactgcat ggtatggagg acagcatggt gttcgatagc
     1441 cgtagtaatc aggtgttttc ctttggattg atgcgcaagt gcatagcctt gaatagctaa
     1501 agtatcagct tcggagcctc cagatgtgaa aataatttgt tcgggagagg cggagagaat
     1561 ctgagcaatc tcttgacgac tttgtctaag acgttgattg gctttccgac cgctttgatg
     1621 aatactggag gggtttccat aattttcttg agccacttcc atcatccgtt ggagtgcagt
     1681 aggagataga gcagtggttg ctgcattatc taaataaatc aaagagttac tcctagtttt
     1741 ctttttcagg actagtaaat ttgaaaagtg ggctgagtgg ctgatgttct tggatgcggt
     1801 ggatggcatc tgcaatcaag tcactagctg ttaggaagac gatattttta ggatgctgtt
     1861 ctttgcttgc aacagaatcg gtaactaata tttctttgat tggtgcttca tctaagagtt
     1921 gggttgcagt tccagcaaag agaccgtggc tggcaactgc ataaatttca gtagcaccac
     1981 cttcttggac aatttttgaa gcttgtgaga aagtgcgacc agtattgaga atatcgtcca
     2041 ccaaaatagc ttttttacca gcaacatcac ctataatata gccttcagaa cgctcagaat
     2101 catcttgagc gtagtcgata attgcaatcg gtgcatttag aaattcagcg atattacgcg
     2161 cgcgtttgat accagaattt ttaggactca caatgacgac atcttcaccg cagagtcctt
     2221 tttccatgta gtaggctgca aaaagtggtt ctgtaaggag attgtctact ggaatatcaa
     2281 agaaaccttg aatttgtgaa gcgtgcaagt caagagtcaa tacacggtcg acaccagctt
     2341 taaccagcat atttgcaact aatttagctg taattggttc gcgtggagaa gcggtacggt
     2401 cttgacgagc atagccgaaa taagggatga cagctgtaat ggtgttggca ctagcacgtt
     2461 tacaagcatc gatcataatc aataattccc acaagtgatt attgaccgga tagcttgttg
     2521 attggataat gtagacgtct acgccacgta cagattcttc aatgttaatt tgaatctcac
     2581 catcagaaaa ctgacgagaa gacagctttc caagagggat tccagcagaa tcagcaattt
     2641 tttcagcaat atcacggttg ctattgagtg agaataattt aatgttatgt tcaccgagtg
     2701 gttgtaccat tttaaagtaa ctccttttac acactgagct tttatgatga cataaaagta
     2761 ttttttacga tagatagttc tattctatca aaaaaatgaa aaattttcag tagtgaaact
     2821 aaaaaatcaa ccttagttgg ctgatttttt atagttattt catgtgggca gcagttcgtg
     2881 cgaccttact actagcatat ttgaattgaa ttgatttctt cttaaggaat tcatcaaaaa
     2941 gaattttccc tgcatctgca tcggtcactt cgacttctaa ttcgtaatct ttttgtccca
     3001 cataatcatt ttcatcgagg gccataaggc ctatagatgt ctgtttctca tagcgttttg
     3061 tcgtgaggat tccccagaca gcaagtttat caagtggaat ttcggttgca gaaataatat
     3121 cagaaatttg tcctgctggt agttgaaaat tttccagtaa atcatgagca gtttggatgt
     3181 ctagaacttg gttatattct tgattgccga cttcttgtgg aatttttaag gttagttctg
     3241 ctgagtcctc aaatgtacga atacggagtg aaaagcggtg attcttcata tctaggtctg
     3301 gggtatcaat gtagtggttt gtttgaagaa ttgggctcac atctgaaaag tcagtaagca
     3361 ggcgtttgta ttcggatttg gtcagtaagg ttttatactc aatttcaagg tggtttttca
     3421 tgcagtaaac cctttcaatg tgttataata gattagtctt tgtgttattc taatacaatt
     3481 taaaagtttt gacaagaaag gggccgtatg gactttaact gggaagaatt tttagacccc
     3541 tatattcaga cagttggtga attgaaaatc aaactgcggg ggattcgcaa gcagtatcgc
     3601 aaggctaatc ggcattctcc aattgagttt gtcacaggac gggtaaaacc gattgaatcc
     3661 attaaagaaa aaatggctct gcgacatatt aaattggaaa atcttgctca agatatgcaa
     3721 gatattgccg gtcttcggat tatggttcaa tttgtggacg acattgaaga agtattagct
     3781 attttgcgga aacgcaagga tatgcaaatt gtgcatgaac gggattatat caataatatg
     3841 aaagcctctg gctaccgttc atatcatgtg gtgattgagt atcctgtgga tactataaat
     3901 ggcaacgaga cggttctggc agaaatccag attcgaaccc tttccatgaa tttctgggct
     3961 acaattgaac attctctgaa ttataaatat aagggaaatt ttccagaaga aatcaagaaa
     4021 cgcttggaag ttacagcaaa aattgcctat gaattagatg aggagatgcg aaaaatccgc
     4081 aacgacattc aagaagcgca ggccttattt gatcctgcct atcgcaaact gaatgacggt
     4141 gtgggcaaca gtgatgatac agatgaagaa tacaggtaga aaaaaaatcg ctctgctcgc
     4201 tagtcgaaat cctaagagtg aagcggtgtc caaagaatta tggacaaagt taaaagaagc
     4261 aaattttatt ttgactccta aaaatccaga tattgtcatt tctatcggtg gagatgggat
     4321 gcttttatct gcttttcaca aatatgaaaa gttgattgat cgtgtacgtt ttgtaggaat
     4381 tcacacgggt catttggggt tttatacaga ttatcgcgat tttgaagtgg acaagctgat
     4441 tgaaaatctt aagcttgata caggtgctag agtatcgtat ccgattttaa atgttaaggt
     4501 caagatgaca gatggtcgca ttgtcgaggc gcgtgcctta aatgaggcga cagtaaaacg
     4561 tctttctaag accatggtcg cagatattat tattaacaat gtgcccttcg aacgcttcag
     4621 aggggatgga atttcggttt caacgccgac aggctcaaca gcctataaca agtcattagg
     4681 tggtgctgtt ttacatccaa caatcgaggc attgcagatt gcagaagtgg caagtttgaa
     4741 caatcgtgtt taccgcacct tgggttcatc cgttgttgtc cctaaaaaag acaagattgt
     4801 gattgaacca aagcatagtg accgttactc gattgcggta gacaataaga cttttgtcta
     4861 tgatagtatt gaaagtatag agtatcagat agacaatagt aagattcatt ttgttgcaac
     4921 accgagtcat actagttttt ggaatcgggt taaggacgcc tttattggag aggtggagta
     4981 atgcggtttg aattcattgc tgaccagcat acgaaaataa agactttcct taaaaaacat
     5041 ggtgtctcta aaggactatt agcgaagatt aagtataccg gtggcaatat ctgggtcaac
     5101 gatattgagc aaaatgcaac ttacttgctt gatattggag atagggttac aattgacata
     5161 cctgctgaag aggatttgac tggtagtttg aagccgattt tctttccgtt ggatattgtc
     5221 tatgaagatg atcattttct gtcgataaat aagccggttg gctacgcctc cattccttct
     5281 gccttgcatt catccacaat cgcaaatttt gtaaaaggtt atcttattga gcagaattat
     5341 gagaataagc aggttcatat tgtgactcgt ctggataggg atacatcagg cgtcatgctg
     5401 tttgccaagc acggctatgc ccatgcacga ctcgataagc agttacaggc caagcttatt
     5461 cataaacgtt attatgcttt ggtgaagggg aatgggcagc tagaagagac aggggatatt
     5521 attgccccta tcggtcgtcc agaagatagc atcattaccc gttgtgtgac aaagaatgga
     5581 aaatatgccc atacttccta tcgtgttgtg caatcgtggg gagatattca tttagtagat
     5641 attcaactac atactgggcg gactcatcag attcgtgttc atttttctca cattggattt
     5701 ccgttgttag gagatgatat gtatggtgga agtttggaat gtgggataga acgtcaagcc
     5761 ttacattgtc ataatttggc cttcgacaat cctttttcag ccgaaaggat tgatttggaa
     5821 gcaccgcttc ctgaggattt tcaggctgtt atcaatcagt taataagtaa ataaatttaa
     5881 ggagtttgtt catgaaaatt tttgagcaat tagctacaaa gctagagggt aaaaaagtac
     5941 gtatcgtatt gccagaaggt gaagaaccac gtatcttgca ggcgacaaaa cgtttggtaa
     6001 atgagtcgga tgtagtaccc gtcttgcttg gaaacccaga tcgtattaag atttaccttg
     6061 acattgaagg gattacagaa ggttttgaaa ttattgaccc agctcattat gatagatatg
     6121 atgagatggt agctgctttg gtagagcgcc gtaaaggtaa ggtatcagaa gagcaggcac
     6181 atgagttgtt gaaagatgtt aactactttg gtgttatgtt ggtatatatg ggcgtagtag
     6241 aaggtatggt atctggtgcg attcattcaa ccgcttcaac agttcgtcca gctcttcaaa
     6301 tcattaaaac agagccaggt gtatcgaaaa catcaggtgc cttcttgatg gtaaaaggtg
     6361 aagagcgtta tatttttggt gactgtgcta tcaacattga tccagatgct caaaccttgg
     6421 cagaaatcgc tatcaactca gcacgtactg ctaaaatgtt tggtattgat cccaaagtag
     6481 cgatgctcag ctattctaca aaaggctctg gtgctggtcc taaagtagat aaggttgttg
     6541 aagctacaaa aattgctcaa aaattgcgtc ctgagttgga tttggacggt gaattgcaat
     6601 ttgatgctgc ctttgttcct accacaggta aattaaaagc cccaggttcg agtgttgctg
     6661 gtcaagcgac tgtatttatc ttcccaggta tctcagctgg taatattggt tataaaattg
     6721 ccgaacgtat gggtggattt gcagcggtag gtcctgtttt acaaggtttg aatcagcctg
     6781 taaacgactt gtctcgtggt tgtaaccctg atgatgttta caacttgacc ttgattactg
     6841 cggttcaggc tttggagcac cgttaatatg aaattttta
//
LOCUS       DBNFEG010000119         6571 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.119, whole genome shotgun sequence.
ACCESSION   DBNFEG010000119 DBNFEG010000000
VERSION     DBNFEG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.119"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..705)
                     /locus_tag="AC1PNG_001578"
     CDS             complement(<1..705)
                     /locus_tag="AC1PNG_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HIP6702075.1"
                     /translation="MSFSVISSYLIQHPVLTALLIAHFLSDFTFQSQALADDKKLHLK
                     ALFMHLFIVSLPLLILALLTPVQNGDLFFQVWLSHLAIDYVKYFLNKHHWIKDSWEAG
                     AFLADQLLHVISIIILYHTIGVNVASHSLWIEPNYLLLQILFILLISKPVNILFKLYF
                     SKYQVAEGEEEQTVTGAGALIGQLECLIMGIFLLLGQYTAIGLVFTAKSIARYDKISK
                     SQAFAEYYLIGSLFSII"
     gene            complement(709..1377)
                     /locus_tag="AC1PNG_001579"
     CDS             complement(709..1377)
                     /locus_tag="AC1PNG_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HIP6702076.1"
                     /translation="MNYIAIIGDIVNSKQTSNRSSIQERLKQQLNRINHSFAQDFASP
                     FTITKGDEFQALCKPNPYIFLIIDQIQLAFRDEVEIRFGIGLGEILTAIDPKLSIGAD
                     GPAYWEARKAIDFIHDNHDYGTSKIAFSSEYKQIDRVINPLLTSSDFIKAAWNRSQTD
                     LFETLISKKIYQEDFTQKPIAEKMKLSQSAFTKRLKSSGIKLYLRNRQTAMNLILQLS
                     QKEG"
     gene            complement(1571..3145)
                     /gene="ffh"
                     /locus_tag="AC1PNG_001580"
     CDS             complement(1571..3145)
                     /gene="ffh"
                     /locus_tag="AC1PNG_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIP6702077.1"
                     /translation="MAFESLTERLQNVFKNLRRKGKISESDIQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAIGHEVIETLNPAQQIIKIVDEELTAVLGSETSEIIKSPKIPT
                     IIMMAGLQGAGKTTFTGKLANKLKQEENARPLLIAADIYRPAAIDQLKTLGQQIDVPV
                     FELGNQVPALEIVRQGLEQAKANHNDYVLIDTAGRLQIDQALMAELREIKEFAQPNEI
                     LLVVDAMIGQEAANVAREFNDQLAITGVILTKIDGDTRGGAALSVRHITGQPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASKEYDEKKSLELAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMAGNPALANLKVDEREIARKRAIVSSMTPAERENPD
                     LLTPSRRRRIANGSGNSFVEVNKFIKDFNQAKTMMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMNGMDMSALEDMMGGAGMPDMSQLMGGAGMPDMSQMFGGGLKGRIGEFAMKQA
                     MKRQANKIKKAKKKRK"
     gene            complement(3158..3490)
                     /locus_tag="AC1PNG_001581"
     CDS             complement(3158..3490)
                     /locus_tag="AC1PNG_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HIP6702078.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FQVSRQAVYDNIKRTEKLLEDYEMKLHMYSDYVVRSQIFDEILNKYPEDAYLKEKIAI
                     LTSIDNRE"
     gene            complement(3593..4291)
                     /locus_tag="AC1PNG_001582"
     CDS             complement(3593..4291)
                     /locus_tag="AC1PNG_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIP6702079.1"
                     /translation="MKAAYITIHDKIKEQIDNGIWKIGQRLPSERDLADEFGVSRMTL
                     RQGITLLVEEGILQRKIGSGTYVASTRVQEKMRGTTSFTELVQLQGKTPSSKLLSYTR
                     TKPNEKEVEQLGLSRGEYVIRMERVRYADNVPVVYEVASIPERLIKNVPKEDVTNHFF
                     KTLMDNGYRIGTSKQTIFARLANEKVAQYLQISKNQAILALKQVSYLEDGQAFEFVNS
                     QYVGERFEFYLENN"
     gene            4567..6117
                     /gene="guaA"
                     /locus_tag="AC1PNG_001583"
     CDS             4567..6117
                     /gene="guaA"
                     /locus_tag="AC1PNG_001583"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIP6702080.1"
                     /translation="MTKQDVQKIIVLDYGSQYNQLISRRIREFGVFSELKNHKITAEE
                     VRAINPIGIVLSGGPNSVYAENAFDIDPEIFELGIPILGICYGMQLITHKLGGKVVPA
                     GEAGNREYGQSNLQLKTESALFAGTPEEQLVLMSHGDAVTEIPADFHLVGLSADCPYA
                     AIENTERRIYGIQFHPEVRHSVYGNDILKNFAFGICGAKGDWTMENFIETEIEKIRQT
                     VGDKKVLLGLSGGVDSSVVGVLLQRAIGDQLTCIFVDHGLLRKNEGDQVMEMLGGKFG
                     LNIIRVDAAKRFLDLLAGVSDPEKKRKIIGNEFVYVFDDEASKLTDVEFLAQGTLYTD
                     IIESGTDTAETIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRALGTALGMPDEVVWRQ
                     PFPGPGLAIRVMGEITEEKLQTVRESDAILREEIAKAGLDRDVWQYFTVNTGVRSVGV
                     MGDGRTYDYTIAIRAITSVDGMTADFAKLPWDVLQKISVRIVNEVDHVNRIVYDITSK
                     PPATVEWE"
     gene            complement(6279..>6571)
                     /locus_tag="AC1PNG_001584"
     CDS             complement(6279..>6571)
                     /locus_tag="AC1PNG_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922381.1"
                     /note="McrC protein together with McrB forms the McrBC
                     restriction system; recognizes N4- and C5-methylcytosine
                     (and 5-hydroxy-methylcytosines); appears to act against
                     5-methylcytosine preceded by a purine residue; MrcC
                     modulates the specificty of McrB and has DNA cleavage
                     activity; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-methylcytosine-specific restriction
                     endonuclease system specificity protein McrC"
                     /protein_id="HIP6702081.1"
                     /translation="NTQIRFDKRTLHSNNLYQIFTYVKNQAYRLSDSNDTVAGMLLYA
                     KTDIDIQPNQVYQMHGNQISVKNLDLNLQFASIAEQLDDIITSHFVSPPKRY"
BASE COUNT         1872 a         1298 c         1331 g         2070 t
ORIGIN      
        1 aatgatacta aatagcgaac caattaaata gtattcagca aaggcttggc ttttggatat
       61 tttatcatag cgtgcgatgg attttgctgt aaataccagc ccgatagctg tgtattgccc
      121 tagaagtaga aaaatcccca taatcaagca ttctaattgg ccaatcaagg ctcctgcacc
      181 tgtcacagtc tgctcttctt ctccttccgc tacctgatat ttgctaaagt agagtttgaa
      241 aagaatattg acgggcttgc tgatcagaag gataaaaagg atttgcagga ggagataatt
      301 tggttcaatc cagagtgagt gtgaagcaac attgacaccg atagtgtgat aaagaatgat
      361 gatagagatg acatgtagca attgatcagc taggaatgct ccagcttccc aggagtcctt
      421 tatccaatgg tgtttgttga gaaaatattt gacatagtca atcgcaagat ggctcagcca
      481 aacttgaaaa aataaatctc cgttttgaac gggggtaagt aaggctagaa taagaagagg
      541 gagagaaaca atgaaaaggt gcatgaaaag agctttcaaa tgcagttttt tatcatccgc
      601 cagtgcttga ctttggaagg taaaatcact taaaaagtgg gcaataagca aggcggttaa
      661 aactggatgt tgaataagat agctagaaat aactgaaaat gacatgcttt atccttcttt
      721 ctgactgagc tggagaatca aattcatggc agtttgacga ttacgcaggt agagtttgat
      781 tccgctggat ttcaaacgtt tggtaaaggc gctttgactg agtttcattt tctcagcaat
      841 tggcttttgt gtgaaatctt cttgatagat ttttttggag attaaggtct caaacagatc
      901 cgtttgcgaa cgattccagg cagctttgat gaaatcgcta gaagttagca aggggttaat
      961 cacacgatcg atttgcttgt attcagatga aaatgcaatt ttgctggtgc catagtcatg
     1021 gttgtcatga atgaagtcaa tagccttgcg tgcttcccaa taagctggac catctgctcc
     1081 aatacttagc ttgggatcga tagctgttag aatctcaccc aaaccaattc caaatcgaat
     1141 ctccacctca tcacggaagg ctagttggat ttggtcaatt attaaaaaaa tataagggtt
     1201 aggcttgcat aaggcttgaa attcatctcc ctttgtaatg gtaaatggtg atgcgaagtc
     1261 ctgagcgaag gaatgattga ttctattcag ctgctgtttt agccgctctt gaatactaga
     1321 gcggttggaa gtttgttttg aattgacaat atctcctatt attgcgatat aattcataac
     1381 ttgtcctctg attcctatta tatttccatt ataacggaca atataaagat tgtccactaa
     1441 aaaggacatt tttgaatatt tccgttataa cggaaaatgt tggggttcaa aaatcatttt
     1501 tcctgctttg ctacttctat tttacattgc tgtatcttac ttcttcgaat aaaaagtaaa
     1561 aaagaagaga ttattttctc ttctttttgg ctttcttgat tttattggct tggcgtttca
     1621 tggcttgttt catagcaaac tcgccgattc tacctttcaa accgcctcca aacatttggg
     1681 acatatctgg cataccagct cctcccatca gttggctcat atcaggcatg cctgcaccac
     1741 ccatcatatc ttccagagca gacatatcca taccgttcat gtttggcatg tttttaggga
     1801 gattatttgg gttgattccc atttgtttca tcattttgtt catgtcgcct gacatgacac
     1861 cctgcatcat ggttttcgct tggttgaaat ccttgataaa tttattgact tcgacaaagc
     1921 tatttccaga accgttagca atacgacgac gacggctcgg tgtcaacaaa tcaggatttt
     1981 ctcgttcggc tggtgtcata gaagatacga tagctcgttt tcttgcaatt tcacgctcgt
     2041 caactttcaa gttggcaagt gctgggttac cagccatacc aggaatcatc ttcagcaggt
     2101 cttccattgg tcccatattt tgaacttggt ccagctgatc aatgaaatcg ttgaaatcaa
     2161 aggtattctc acgcattttt tcagcgagtt ctagtgattt cttctcatcg tattctttgc
     2221 tggctttttc aatcaaggtc agcatgtcac ccatgcccaa aatacgcgag gacatacggt
     2281 ctgggtggaa ggtttcaata tctgtgattt tttcaccagt accggtgaat ttgattggct
     2341 gaccagtaat gtgacggaca gacaaggctg caccaccacg tgtatcaccg tcaatcttgg
     2401 tcaaaatcac accagtaata gccagttgat cattgaactc acgcgcaacg ttggcagctt
     2461 cctgaccaat catggcatcg acgaccaaga ggatttcgtt tggctgagca aactctttga
     2521 tttctctcaa ttcggccata agggcttggt caatctgcaa acgaccggcc gtatcaatca
     2581 gaacgtagtc attatgatta gctttggctt gttctaaacc ttggcgtaca atctccaggg
     2641 cagggacttg attgcccaac tcgaatacag gtacatcaat ttgttgacca agggtcttca
     2701 actggtcaat ggcagctgga cgataaatat cggcagcaat caagagtgga cgggcatttt
     2761 cttcctgctt gagtttatta gccaacttac ctgtgaaggt cgttttacca gccccctgca
     2821 atccagccat cataatgata gttggaattt ttggtgattt gataatttct gaagtttcag
     2881 aacctaatac agccgtcaat tcttcatcaa cgattttgat gatttgttga gcggggttca
     2941 gggtttcaat gacttcatga ccgatagcac gttcacgcac cttcttgata aagtctttta
     3001 caactggcaa ggcaacgtcg gcttctagta aggccagacg aatttcctta gttgcttcct
     3061 gaatatcact ctcagaaatt ttccccttac gacgcagatt tttaaaaaca ttctgtaaac
     3121 gttctgttaa gctttcaaaa gccatatact tctcctctta ttctctatta tcaatactgg
     3181 ttagaatagc gattttttcc ttcaaataag catcttctgg atacttgttc agtatttcat
     3241 cgaaaatctg gctacgcact acatagtcag aatacatatg tagcttcatc tcgtagtctt
     3301 ccagcaattt ttctgttcgt ttaatgttat catagaccgc ctgacggctg acttgaaatt
     3361 cttcagcaat ctcagccaaa ctgtaatcat cggcataata gagctcgatg tagttcattt
     3421 gcttgtctgt tagcaaggct gcatagaatt caaataaggc attcattcgg ttggtttttt
     3481 caatttccat aaactttatt atatcaaaaa ttccatattt tataaataag aaaatccgtc
     3541 atcactagaa agattacgga ttttaaaaat gttttacata attattttta tgttaattgt
     3601 tttctaaata gaattcgaaa cgttccccaa catactgact atttacaaat tcaaatgctt
     3661 gaccatcctc gagataagat acctgtttta gtgcgagaat agcctggttt ttagaaattt
     3721 gtaaatattg agccactttt tcgttggcta aacgcgcaaa aatggtttgt ttgctcgttc
     3781 caattcgata accattgtcc ataagtgttt taaagaaatg gttggtaaca tcttcttttg
     3841 ggacattttt tatcaagcgt tcaggaatgc tggcaacctc atacacgacg ggaacattat
     3901 ctgcatagcg gacccgctcc attcgaataa catactcgcc acgggataaa cctaattgtt
     3961 caacttcttt ttcattaggt ttggttcgcg tataggacaa taatttgctg cttggcgtct
     4021 ttccttggag ttgtactaat tcagtaaagg aagtcgtgcc tcgcatcttt tcttgaaccc
     4081 gtgtactggc gacataagta ccggaaccaa tcttgcgttg tagaatacct tcttccacta
     4141 aaagtgtgat tccttgacgg agcgtcatac gagaaacacc aaattcatct gctaaatcgc
     4201 gttcacttgg taaacgttga ccaattttcc agattccgtt atcgatttgt tccttaattt
     4261 tatcgtgaat tgtgatatat gctgctttca tttatctagc ctttctcttc tttctagttt
     4321 accaaaaata aaggcgtttg gctaattcag gccacactct attggtaaaa ttctcctagg
     4381 caaacaatga agtcagatta cctaaaataa agtgagtaaa aacaataaaa atacgaacac
     4441 taacattttt attatcactc atagtttgca aaacagatat ttaacaaata aaatttggaa
     4501 aatgttcgct tttatgttaa aataactact agtatttgaa tttttttcaa aacgaaaggg
     4561 aaaaacatga caaaacaaga tgtccaaaaa attattgtat tggattatgg tagccagtac
     4621 aaccaactta tttcacgtcg aattcgtgaa tttggtgtct tttcagaatt gaaaaaccat
     4681 aaaattacag ctgaggaagt tcgtgctatc aatccaattg gtatcgttct ttcaggtgga
     4741 ccaaactctg tctacgcaga aaatgccttt gatattgatc cagaaatctt tgaattgggt
     4801 attcctatct tagggatttg ctatggtatg cagttaatta cccataaatt gggaggtaag
     4861 gttgttcctg ctggtgaggc tggaaatcgt gaatatggtc aatcaaatct tcaactgaag
     4921 actgaatcag cgctttttgc tggaacacca gaagaacagc ttgtactcat gagccacggg
     4981 gatgctgtaa ctgaaattcc tgctgatttc cacctggttg gtctgtctgc tgactgccct
     5041 tacgctgcca ttgaaaacac ggaacgccgt atctacggta tccagttcca cccagaagtt
     5101 cgtcattccg tatatggaaa cgatattttg aaaaactttg cgtttggtat ctgtggagcc
     5161 aaaggcgact ggacaatgga aaacttcatc gaaacagaaa ttgaaaaaat ccgccagact
     5221 gttggggaca agaaagtctt gctcggttta tcaggcggtg tggattcatc tgttgttggt
     5281 gtgcttcttc aacgtgctat tggtgaccaa ttgacctgta tcttcgttga ccacggtctt
     5341 cttcgtaaga atgaaggcga tcaagttatg gaaatgttgg gcggtaaatt tggcctcaat
     5401 attattcgcg tcgatgctgc taagcgtttc ttggatctac ttgctggcgt atctgaccct
     5461 gagaagaaac gtaaaatcat cggtaatgaa tttgtttacg tatttgacga tgaagcgagc
     5521 aagttgactg atgttgaatt cctagcgcaa ggaacacttt ataccgatat tatcgagtca
     5581 ggaacggata ctgctgagac aatcaaatct caccacaacg ttggtggtct gccagaagat
     5641 atgcagttca agttgattga accgctcaat actctcttta aggatgaagt tcgtgctctc
     5701 ggtactgctc ttggtatgcc ggatgaagtt gtatggcgcc aaccattccc aggaccaggc
     5761 cttgccattc gtgtcatggg tgaaatcact gaggaaaaac ttcaaactgt tcgcgaatct
     5821 gacgctatct tgcgtgagga aattgcaaaa gcaggtcttg accgcgatgt gtggcaatat
     5881 ttcacggtta atactggcgt tcgctccgta ggtgtcatgg gagatggtcg tacctacgac
     5941 tacaccatcg ccatccgtgc tatcacttct gtcgatggaa tgacagctga ctttgccaaa
     6001 ctaccatggg atgttcttca aaaaatctct gtccgtatcg taaacgaagt cgaccacgtc
     6061 aaccgcatcg tctatgatat aacaagtaaa ccaccagcaa cagttgagtg ggagtaaatt
     6121 agtccagtgg actaatttac ccgatgccta aaaataaaaa agcatcgctg atctgggaaa
     6181 tcaattaacc agacgctcca aaatataaaa aaacaatcaa agagtagttt agtgctacta
     6241 ctttgattgt ttttttgttt tctagcttaa agctctaact agtaccgttt tggaggggat
     6301 acaaaatgcg atgtaatgat gtcatccagt tgttcagcaa tacttgcaaa ctgcaggttt
     6361 aagtctaaat ttttgacact aatctggttt ccatgcattt ggtagacctg gttaggttga
     6421 atatctatat cggttttagc gtatagaagc attcctgcta ctgtatcgtt tgaatctgac
     6481 aggcgataag cctggttttt cacataggta aaaatttggt atagattatt tgaatggagt
     6541 gtgcgcttat caaagcgaat ctgtgtgtta c
//
LOCUS       DBNFEG010000120         6569 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.120, whole genome shotgun sequence.
ACCESSION   DBNFEG010000120 DBNFEG010000000
VERSION     DBNFEG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.120"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..591)
                     /gene="nth"
                     /locus_tag="AC1PNG_001585"
     CDS             complement(<1..591)
                     /gene="nth"
                     /locus_tag="AC1PNG_001585"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIP6702082.1"
                     /translation="MVLSKKRARKVIEEIIALYPDAKPSLDFRNHFELVCAVLLSAQT
                     TDAAVNKATPGLFEAFPTPQAMAAAQVKDIEPYISRLGLYRNKAKFLKECAQQLLDRH
                     DGIVPQTREELEALAGVGRKTANVVLSVGFGIPAFAVDTHVGRICKHHDIVKKSATPL
                     ETEKRVMEVLPPELWLPAHQAMIYFGREVCHPKNPEC"
     gene            689..1540
                     /locus_tag="AC1PNG_001586"
     CDS             689..1540
                     /locus_tag="AC1PNG_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIP6702083.1"
                     /translation="MDLQELCRKVIQARPRNSHKGSYGRVLLIGGLYPYGGAIIMAAL
                     ATVNSGAGLVTVATEKDNIPALHSHLPEAMAFSFDDQALLISSLENADLVLIGPGFGE
                     NSRAEQLLDFVFEHLSDQQILVLDGSALTLVAKQNRKNFPCKQVILTPHQKEWERLSS
                     IPIADQTSQRNLEALQSFQENTILVAKSSATQILKTQPNQVTPIGAGGPYQATGGMGD
                     TLAGMIAGFAVQFPHVQLYERILVATYLHSYIADQLAQDYYLVKPTDVSRNIQKTMLQ
                     FQTQTTA"
     gene            complement(1722..6479)
                     /locus_tag="AC1PNG_001587"
     CDS             complement(1722..6479)
                     /locus_tag="AC1PNG_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIP6702084.1"
                     /translation="MKKKETFSLRKYKIGTVSVLLGAVFLFAGAPSVAADELTSLVET
                     KVEATVPDVIVSESASESPVVEELVDTSVEATPTDVTTTDNVEETLGSEALENITNTE
                     VEATQPAVETPAISEKKVEEDEKLAVADETTAITNQEEAKPQNIDSNTIITVPKVWDS
                     GYKGEGTVVAIIDSGLDVDHDVLHISDLSTAKYKSEKEIEAAKEAAGITYGEWFNDKV
                     VFGYNYVDVNTVLKEEDKRSHGMHVTSIATGNPTQPVAEQLMYGVAPEAQVMFMRVFS
                     DLKATTGAALYVKAIEDAVKLGADSINLSLGGANGSVVNMNENVTAAIEAARRAGVSV
                     VIAAGNDGTFGSGHSNPSADYPDYGLVGTSSTARDAISVASYNNTTVGSKVINIIGLE
                     NNADLNYGKSSFDNPEKSSVPFEIGKEYEYVYAGIGQASDFDGLNLTGKLALIKRGTI
                     SFSEKIANATAAGAVGVVIFNSRPGEANVSMQLDDTAIAIPSIFIPLEFGEALAAKPY
                     KIAFNNETDIRPNPKAGLLSDFSSWGLSADGELKPDLAAPGGAIYAAINDNDYANMQG
                     TSMASPHVAGAAVLVKQYLQATYPTKSPQEIEALVKHLLMSTAKAHVNKETTAYTSSR
                     QQGAGIIDTAAAISTGLYLTGEDGYGSITLGNVEDTFSFTVKLHNITNEDKTLNYSTQ
                     LTTDTVQNGLITLAPRLLAEIPGGKVTVKANSSTTVTINVDASSFAEELTGLMKNGYY
                     LEGFVRFTDVADGGDIVSIPYVGFRGEFQNLAVLEEPIYNLIADGKGGFYFEPVTAQP
                     DTVDISHHYTGLVTGSTELIYSTDKRSDFAIKTLGTFKNEAGYFVLELDESGKPHLAI
                     SPNGDDNQDSLAFKGVFLRNYTDLVASVYAADDTERTNPLWESQPQSGNKNFYSGDPK
                     NPKSSIIYPTEWNGTDSEGNALADGKYQYVLTYSSEVPGAAVQTMIFDVIIDRESPVI
                     TTATYDETNFTFNPRPAIEKGESGLYREQVFYLVADASGMTTIPSLLENGDVTVSDNK
                     VFVAQNDDGSFTLPLDLADISKFYYTVEDYAGNISYEKVENLISIGNEKGLVTVNILD
                     KDTNSPVPILFSYSVTDETGKIVAELPRYAGDTSVLKLPFGTYTFDLFLYDTEWSSLA
                     GETKAVVTISEENSTAEVNFYVTLKDKANLLVDIDALLPSGSTIQLVTADGQTIQLPN
                     AKYSKTDYGKFVPVGTYTILPTLPEGYEFLEELDVAVLANQSNVKKLTLINKVALKEL
                     IAELAGLEETARYYNASPELQTAYDKALEDANAVYANKHNQAQVDSAVASLVAAREQL
                     NGQATDTEKLIAEVSNYTPTQANFIYYNAENTKQIAYDTAVRSAQLVLNQENVTQAVV
                     NQALADLLAAKANLDGQKTDISALRSAVSVSSVLKATDAKYLNASENVKQAYDQAVEA
                     AKAILADESASQASVDQALAVLTSAQAELDGVATSTNDAKEPANTATDKKDEGTVTPP
                     PIDSEKVDVQAPPVKDTGNSGHVPIGQKPNPQPTLPRPVTLQASLSSPNQEKQVTQLP
                     NTGDNDTRYYLVLGVIIGLGTLLVSKRRHKEEV"
BASE COUNT         1807 a         1408 c         1331 g         2023 t
ORIGIN      
        1 gcattcaggg ttcttaggat ggcaaacctc acgaccaaaa taaatcatag cttgatgagc
       61 agggagccag agttcaggag gtaagacttc catgactcgt ttttctgttt ccaggggtgt
      121 agccgatttt ttgacaatat cgtggtgttt acaaatccgt cccacgtggg tatcaactgc
      181 gaaggcggga attccgaaac cgacacttaa aacaacattg gccgtttttc ttcccactcc
      241 tgccaaggct tctaattcct cacgagtctg tgggactatg ccatcatgtc ggtccaacaa
      301 ctgttgggca cattctttta aaaactttgc tttattacga tataagccca agcgagatat
      361 atagggctcg atgtccttca cttgtgctgc tgccatggct tgaggcgttg ggaaagcctc
      421 gaacaaacct ggggttgcct tattaacggc agcatccgtt gtctgagctg acaaaagtac
      481 tgcgcagacc aattcaaaat ggttgcggaa atccagacta ggcttagcat caggatagag
      541 ggcgatgatt tcttcaataa cttttcttgc acgttttttt gataatacca tgaattcatt
      601 atagcatgaa aacttggatt tatctaaaat ctggattgtc ataaacctca ttcctcgtgg
      661 tataataaga agaaaagggg gaatatgtat ggacttacaa gaactctgta gaaaggtcat
      721 tcaagcaagg ccacgcaaca gccataaggg aagctatggt cgagtcttgc tgattggtgg
      781 actgtatccc tatggtggag cgattatcat ggccgcacta gcaaccgtca acagtggagc
      841 aggcttggtg actgttgcta ctgaaaaaga taacatacca gccttacata gtcatctgcc
      901 tgaagccatg gcattttcat ttgacgatca agccttgctg ataagcagct tagaaaatgc
      961 tgacttggta ttgattggtc caggttttgg ggaaaatagc agagctgaac agttattgga
     1021 ctttgttttt gaacatttat ctgaccagca gattttagtt cttgacggct cagcccttac
     1081 cttagtcgca aagcaaaaca gaaagaactt cccgtgtaaa caagtcatcc tgacacccca
     1141 ccagaaagaa tgggaacggt tatcatccat tccgattgct gaccaaacaa gccaaagaaa
     1201 tcttgaagcc ttgcaatctt ttcaagaaaa tacaattctt gtagctaaaa gttctgctac
     1261 acaaatccta aaaacccagc caaaccaagt caccccaatt ggagctggtg gtccttacca
     1321 agcaacagga ggtatgggag atactcttgc aggtatgatt gcaggatttg ctgtacaatt
     1381 tccccatgtt caactatatg aaagaatcct tgtagcaacc tacctccact cctatattgc
     1441 cgaccaactg gctcaagatt attatcttgt aaagccaact gatgtctcaa gaaacatcca
     1501 aaagacaatg cttcaatttc aaacacaaac aaccgcctaa tcaatgatta agcggtcttt
     1561 tatataacaa actcgtgatt aaagcctgac aaatggtaga aaatgatact caatgcaaat
     1621 caaaagtagc ctaggaaacg aagccgaaga tagaactgga gttcatcaag gcaagttgac
     1681 aacggataat ttagattttc gaagagtatg actatatttt cttagacttc ttctttatga
     1741 cgtcgtttgc ttaccaacag agtccctagc ccaataatga caccaagaac aagatagtat
     1801 ctcgtatcat tgtctccagt atttggtagt tgagtcacct gtttttcttg attagggcta
     1861 gatagactag cttgcaaagt gaccggacga ggtaaagttg gttgagggtt tggcttctga
     1921 cctatcggta catgccctga attcccagta tcttttacag gaggtgcctg tacatcaact
     1981 ttttctgagt ctataggtgg aggcgttaca gtgccttcat cttttttgtc agtggcagta
     2041 tttgctggct ctttggcatc atttgttgaa gtagcaacac catccagttc tgcctgagcg
     2101 cttgtcagaa cggctagagc ttgatcgaca cttgcttggc ttgcagattc atcagctaga
     2161 atcgctttcg ctgcttcaac tgcctggtca taagcttgtt tcacgttttc agatgcattg
     2221 agatacttag catctgtcgc ttttaatacg gaagaaacgg atactgcgct acgaagggct
     2281 gaaatatcag tcttttgacc atctaagttg gctttcgccg ctaacaagtc cgccaacgct
     2341 tggttgacaa ctgcctgtgt tacattctct tggttcaata caagttgtgc tgaacgaaca
     2401 gctgtatcat aggcaatttg tttggtattt tcagcattgt aataaataaa gtttgcctga
     2461 gtcggtgtgt agtttgatac ttcagcaatt agtttttccg tatcggtagc ctgaccgttt
     2521 agctgttctc tcgccgccac aagactggca actgctgaat ctacttgtgc ctgattgtgt
     2581 ttgttggcat atactgcatt ggcatcttct aatgctttat cataggcagt ttgaagttct
     2641 ggactagcat tgtaataacg cgctgtttct tcaagtcccg caagttcagc aatcagttct
     2701 ttcaaagcaa ctttattaat caaggttaat ttcttaacat ttgactggtt tgcaagtact
     2761 gctacgtcta attcttccaa aaattcatag ccttctggga gggttggaag gatagtgtag
     2821 gtaccaactg gtacaaattt accataatcg gtcttagaat atttagcatt tggtagctga
     2881 atagtctggc catcagcagt taccagttgg atggttgaac cagaaggtag taatgcatca
     2941 atatctacca gcaagttggc cttatctttc aaagtcacat agaaattcac ctcggcagtg
     3001 ctattttctt ccgaaatcgt cacgactgct tttgtttcac ctgctaggct tgaccattct
     3061 gtatcatata agaataaatc aaaggtgtaa gtaccaaatg gtagcttaag aacgctagta
     3121 tcgccggcat atcgtggtaa ttctgcaaca atcttgcctg tttcatctgt gactgagtaa
     3181 gagaaaagta ttggtacagg actatttgta tctttatcaa gaatattgac agttaccaac
     3241 cctttttcat tgccgatact gatcagattc tctacttttt catagctgat gttaccagca
     3301 taatcctcta ctgtgtagta gaattttgaa atatctgcaa ggtcaagagg caatgtaaag
     3361 gagccatcgt cgttttgtgc cacaaatacc ttgttatcag aaacggttac atcaccattt
     3421 tctaataagg aaggaatagt tgtcataccg cttgcatctg ctacaagata gaatacttgc
     3481 tcgcgatata gcccggattc tcctttttca atggctggac gagggttaaa tgtaaagttt
     3541 gtttcatcat aggtagctgt ggtgataact ggtgattctc tatcgatgat aacgtcgaaa
     3601 atcatagttt gtactgctgc acctggaact tcagatgagt aggtcaaaac gtattgatac
     3661 ttaccatctg ctaaagcatt tccctcgctg tctgtcccat tccattcagt agggtaaata
     3721 atgcttgatt ttggattttt aggatcacca ctatagaagt tcttattgcc tgactgtggt
     3781 tgactttccc aaagtggatt tgttcgttcg gtatcatctg cagcatagac gcttgcgact
     3841 aaatccgtat aatttctcaa gaagacacct ttgaaagcga gcgaatcttg gttgtcatcc
     3901 ccatttggcg agatagctaa atgaggctta ccagactcat caagctctaa aacaaaatat
     3961 cctgcttcat ttttaaatgt accaagtgtc ttgatcgcaa agtcagatcg tttgtctgta
     4021 gaatagatta actccgtact tcctgtaaca agacctgtgt agtgatggct gatgtcaaca
     4081 gtatctggtt gtgctgtaac aggttcaaag tagaagcccc ccttaccatc ggcaataaga
     4141 ttgtaaatcg gctcttctag aacggctaga ttttggaatt ccccacggaa accaacgtat
     4201 ggaatgctga caatatcacc gccatcggct acatctgtaa atcgaacaaa accttcaaga
     4261 tagtaaccgt ttttcattaa acctgtcaat tcttctgcaa agcttgatgc atcgacatta
     4321 attgtaacag ttgtacttga attggctttc acagtcacct taccgccagg aatctctgct
     4381 aatagacgcg gagccaaggt gatcaatccg ttttggacag tatccgttgt taattgcgtt
     4441 gagtagttta aagtcttatc ttcgtttgta atgttatgaa gtttgaccgt aaagctgaat
     4501 gtatcctcaa catttcccaa ggtaatgctg ccataaccgt cttcgccagt caaatataaa
     4561 cctgtagaaa tagctgccgc agtatcgatg atacctgcac cttgttgacg agaggaagtg
     4621 taggctgttg tttctttgtt cacatgcgct ttagcagtag acataagcaa gtgttttact
     4681 aaggcttcga tttcttgagg ggacttagta gggtaagttg cctgtaaata ttgttttact
     4741 agtacggctg ctcctgctac gtgtggtgaa gccatacttg ttccctgcat gttggcatag
     4801 tcattatcat tgatggctgc ataaatagca ccacctggag cagctaagtc tggttttagc
     4861 tcgccatccg ctgatagacc ccaacttgaa aaatctgaaa gaagacctgc tttggggtta
     4921 ggacgaatgt ctgtttcgtt attgaacgca atcttatatg gtttagccgc taaagcttca
     4981 ccgaattcca atggaatgaa gatagatgga attgcgatag ctgtatcatc cagttgcatg
     5041 ctcacattgg cttcacctgg gcggctattg aaaataacga cccctactgc ccctgcagct
     5101 gttgcattgg ctattttttc tgagaaactg atggttcctc gtttaataag cgcaagtttt
     5161 ccagtcaaat ttaaaccatc aaaatccgaa gcttgaccga ttcccgcata gacatattca
     5221 tattctttcc cgatttcaaa tggtacggaa cttttctctg gattatcaaa cgaactttta
     5281 ccgtaattca agtcagcatt gttttctaag ccaatgatat taattacttt actaccaacc
     5341 gttgtgttat tgtaagaagc gacagaaatt gcatcgcgag ctgttgaaga tgtaccgacc
     5401 aagccataat ctggataatc ggctgacgga ttagaatgac cggaaccaaa tgttccatca
     5461 ttaccagctg caataacaac agaaacccct gcacgacgag cagcctcgat tgctgcagtc
     5521 acattttcat tcatgttaac aacagagcca ttagctcctc ccaggctgag gttgatgcta
     5581 tctgcaccta attttacagc atcttcaatc gcctttacgt acaatgctgc gcctgttgta
     5641 gctttgaggt ctgagaatac acgcataaac atgacttgcg cttcaggagc tacaccatac
     5701 attaattgtt cagcgactgg ttgtgtcgga tttcctgtgg caatactcgt tacgtgcata
     5761 ccgtgtgagc gtttgtcttc ttctttcaag acagtattca catcaacata gttataacca
     5821 aatacaacct tatcgttaaa ccattcaccg taggtaattc ctgctgcttc tttagctgct
     5881 tctatctctt tttctgattt atattttgca gttgagagat ctgaaatatg caatacatcg
     5941 tggtcaacgt caagacctga atctatgatt gcaactactg ttccttcgcc cttgtaacca
     6001 ctatcccaaa ctttaggtac tgtaatgatc gtattgctat caatgttttg tggttttgct
     6061 tcttcctgat tagtaatagc agtagtctca tctgctacgg caagcttctc atcttcttct
     6121 acttttttct ctgaaatagc tggagtttct acagctggtt gagtcgcttc tacttctgta
     6181 tttgtgatgt tttcaagagc ttctgagcca agtgtttcct ctacattatc tgtagtggtt
     6241 acatcagttg gggtagcctc cacagaagtg tcaactaact cctcgactac gggactttct
     6301 gaggctgatt cgctgacgat tacgtcagga acagttgctt ccaccttagt ctctacaagg
     6361 cttgtcaatt catctgcagc taccgatggt gcacctgcaa acaaaaaaac tgcacccaaa
     6421 agaacagaca cagttccaat tttatacttc cgaagtgaga aagtttcttt ctttttcata
     6481 acttttcctc ctagtttttc tccttcatta ttggcaaaac caataagtat attaaaacat
     6541 aaaactatca gcattgcaag tatattctg
//
LOCUS       DBNFEG010000121         6569 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.121, whole genome shotgun sequence.
ACCESSION   DBNFEG010000121 DBNFEG010000000
VERSION     DBNFEG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.121"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            13..813
                     /locus_tag="AC1PNG_001588"
     CDS             13..813
                     /locus_tag="AC1PNG_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIP6702085.1"
                     /translation="MKKILALAATVLAGLTLAACSSTSSQSALDKIKEKGTLVVATSP
                     DYAPFEFQALVDGKNEVVGADIMLAQKIADELGVKLEVSAMSFDNVLSSVQNGKADIA
                     IAGLSYSEERAKVFDFSESYYQISDVLLIKKDSANSLTSIDAMSGKTLAVQKGSTQES
                     YAQENISQANLISLTLMGEAVNELKSGKVDAILMDSPVAAGYVSQNSDLAVASVEFPT
                     IDENSKVIALPKGSTELKTAIDKVIAEVKASDEFDTFLEKAATYASVE"
     gene            complement(941..2779)
                     /gene="glgB"
                     /locus_tag="AC1PNG_001589"
     CDS             complement(941..2779)
                     /gene="glgB"
                     /locus_tag="AC1PNG_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIP6702086.1"
                     /translation="MTEFTDLDLYYMNAGEHTTLYEKMGAHIIKERNKIIGTQFRVYA
                     PNAREVFLVGNFNEWQRSHSMEREIDGVFQLYVDGLKSLEDYKYLIITHDGREIYKAD
                     PYAFFSQVRPDTASTTYNSRYKFKDDLWMYNRVNYDFKEQPVSIYEVHLGSWKQKFAN
                     KNEGGAPVEAFNSYKEITPLLIEHIKENNYTHIELLPITEHPLDASWGYQVTGYYSPT
                     SRYGKPDELKYLVDQCHQAGIGVILDWVPLHFCKDAHGLYQFDGSWLYEYSYEQDREN
                     HQWGTANFDLGKGLTRSFLLSNLKYWLEYFHFDGIRVDALSYLLYWRGETDEDKINHA
                     AIDFIKRVNAMVHTDYKGVLMIAEDSSSFPKVTHSLEDGGIGFDMKWDLGWMNDTLKF
                     VERPAIYRKYHSNEITFGMYYNQNEQFLLPLSHDEVVHGKHSIIEKMNGDYEDKFHLA
                     RVYYSFYFAHPGKKLLFMGNEWGHIREWHEYTEMDWGLLQFPIHHSFYQMMKTLSTFY
                     KEHDAFWKYDYQAYEKGFNWVKIDEEASLYAFSRTSDVQEILTIHNFNDQELFDVHLD
                     LPADSEYKLVFSSNAIPMDTFSLYPDENGARITVPRLTSFYLERVK"
     gene            complement(2852..4297)
                     /gene="glgA"
                     /locus_tag="AC1PNG_001590"
     CDS             complement(2852..4297)
                     /gene="glgA"
                     /locus_tag="AC1PNG_001590"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HIP6702087.1"
                     /translation="MTKKSVLFVASEGLPFIKTGGLADVIGSLPKELVKQGLDVRVVL
                     PLYKKIAVNNHSDFDYVSSFDVRAGDIQSMANVYSQVVDGVTFYFIEHRDFFERDELY
                     GYDDDAMRFGYFQHATCRLLEALHFFPDVMHTHDWHTAALPFLCRTFYSYREEFRNIK
                     HVFTIHNLAFQGIFHKQALWSALGMDYSYYLDGIARFHDECISFMKLGILYADKVTTV
                     SETYAREILTEEFGENMQHVLELRKYDLVGIVNGIDYDSWDSQTDQYLVRNYSLETIE
                     SKKANKLALQAQFALPQDENVLLIGIVSRLTWQKGFYLLTEVLGHLLQAHVQFVILGN
                     GEEDIENAFNHFKHAYPDKFAFYRGYNEPLAHQIYAASDLFLMPSMFEPCGISQLISM
                     HYGTLPLVRETGGLVDTVAPYNMSTKEGTGFSFGGRDAYNMRQVYDLALQTYYDRPDD
                     WYAMVEQAMKRDFSWTASAEKYIWLYREISG"
     gene            complement(4306..5421)
                     /gene="glgD"
                     /locus_tag="AC1PNG_001591"
     CDS             complement(4306..5421)
                     /gene="glgD"
                     /locus_tag="AC1PNG_001591"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HIP6702088.1"
                     /translation="MLRNTLGIVNIEGNNVHFGDVMKHRGVQAFGFLGRYRLIDFALS
                     NMSNSGITEFQVYMPAEMRSTIQHVGTGKHYNINSKRGTLRLLNSATDPNSVYRHDIN
                     AFTENIHYIENSTKDYVLVAPSYFIYSQDFSKVMDDHIATDADITVLYKNVSDAKENF
                     IGCQTLKFGEDHRVVAFEENHGKYKNRPVSLEAYIMKRTTFIDLIKRANKVSSLYWLK
                     DILRDTVDQYKIMGYAHRDYVACINTTASYFNTQIELIDQDTRKLLFKHDWPIHTQTS
                     DSSPCLYGPLAEVKRSLISNGANINGQVENSVIDRDVIIEEGVVIKNSIILNGVTIKS
                     GANIENAIIDKATKIIHPIDIKGSETSPSYLKPHQII"
     gene            complement(5434..6561)
                     /locus_tag="AC1PNG_001592"
     CDS             complement(5434..6561)
                     /locus_tag="AC1PNG_001592"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIP6702089.1"
                     /translation="MAQNKMLAMILAGGRGTRLEGLTKKVAKPAVAFGGKYRIIDFPL
                     SNCANSGIDIVGVLTQYEPVLLNSYVAQSQRWGLDVQGSGVFVLPPSEKIEGFGLYKG
                     TADAITQNIDFIDLHDPEYVLILSGDHIYKMNYDKLLDTHIQKKADATIAVIEVPIKE
                     ASRFGIMNTDEDYRIEEFEEKPENPKSNLASMGIYIFTWKTLKKYLQEDDKLDTSSHD
                     FGHDIIPKYLEDGRTLIAHPFRGYWKDVGTVNSLWESNMDLIDHAGDLDLSDRSWRIY
                     SEDKGSPAQVIGATATVKSAYIDKGAIIDGTVEHSVISTDVQVNQNAVVKNSVILPGA
                     IIGEGVELDYVIVAENIKIADGVKLTGDIDHILLIDKNVTK"
BASE COUNT         1930 a         1280 c         1360 g         1999 t
ORIGIN      
        1 tatttaggaa atatgaaaaa aatcttggca ttagcagcaa cagttttagc tggactgaca
       61 ttggcagctt gttcttcgac gtcatcacaa tctgcattag ataaaattaa agaaaaagga
      121 acattagtag tggcaactag tccagactat gctccattcg agtttcaggc actggttgat
      181 ggaaagaatg aagttgttgg tgcagatatt atgttggctc aaaaaatagc cgacgaactt
      241 ggggtcaagc ttgaagtttc agcaatgagt tttgacaatg tattgtctag cgttcaaaat
      301 ggcaaagctg atattgcaat tgctggtcta tcttattcgg aagagcgtgc aaaagttttt
      361 gatttctctg aaagttacta tcaaatttct gatgtattac ttatcaaaaa agatagcgcc
      421 aattcactca cttcaattga tgctatgagc gggaaaactc tcgcggtaca aaagggttca
      481 acacaagaat cctatgcaca ggaaaacatt agtcaagcta acctaatttc tttaaccttg
      541 atgggtgaag ctgtaaatga gctcaaatca ggaaaagttg atgctatttt aatggattct
      601 ccagttgctg ctggttatgt ttcacagaat tcagatttag ccgttgcatc cgttgaattt
      661 ccaacgattg atgagaattc gaaagtcatt gctttgccaa aaggtagtac agaactaaaa
      721 actgcgatag acaaggttat cgcagaagta aaagcaagtg atgagtttga tactttcctt
      781 gagaaagctg caacatatgc aagtgttgaa taagagaaat gaacaatcat cggaaacgat
      841 ggttgttttt gcatgcaaaa aagaatggag taagtgagcc acaccccaaa agttagacag
      901 aaaatttggg tcggttcaat actccatttt ttatagataa ttatttcaca cgctctagat
      961 aaaaactggt caaacgtggt acagtaattc tggctccatt ttcatcggga tagaggctaa
     1021 atgtgtccat tggaatagca ttagatgaga agacaagttt atattcgctg tcagctggca
     1081 aatccaagtg tacatcaaat agttcttgat cattaaagtt atgaatagtc aatatttctt
     1141 gcacatcact agtgcgagaa aaagcataca ggcttgcttc ttcatctatt tttacccaat
     1201 taaatccttt ttcataggct tggtagtcat atttccagaa agcatcatgt tctttataga
     1261 aagtagatag tgttttcatc atttgataga aagaatggtg gattggaaat tgtagcagtc
     1321 cccagtccat ttctgtatat tcgtgccatt cacggatgtg accccattca tttcccatga
     1381 agagtaattt ctttccagga tgggcaaagt aaaagctgta gtagactcga gccagatgga
     1441 acttatcttc gtagtcgcca ttcatcttct cgataatgga gtgcttccca tgaacaactt
     1501 cgtcgtgcga aagtggtagt aagaattgct cgttttggtt atagtacata ccaaaagtga
     1561 tttcatttga gtgatatttt cgataaatag cagggcgttc cacgaatttt agtgtatcat
     1621 tcatccagcc cagatcccat ttcatatcaa atccaatacc gccatcttct agagaatgtg
     1681 tgactttagg gaagcttgaa gagtcttcag caatcatcag gactccctta tagtcggtat
     1741 ggaccatggc attgacacgt ttgataaagt caattgcggc atgatttatc ttatcctcat
     1801 ctgtttcacc acgccaatag aggagataag atagtgcatc aacacggatg ccatcaaaat
     1861 ggaaatattc caaccagtat tttaaattag aaagaaggaa agaacgagtc agtccttttc
     1921 caaggtcaaa attagcagtc ccccactggt ggttttcacg gtcttgttcg taggagtatt
     1981 cgtatagcca actaccatca aactggtaga gcccatgagc atctttacag aaatgaagtg
     2041 gtacccagtc aagaatcact ccgattcctg cttggtgaca ttggtcaact aaatacttaa
     2101 gttcatctgg cttgccatag cgactagttg gactgtagta gcctgttacc tgatagcccc
     2161 aagacgcatc gagaggatgc tcggtaattg gcaataactc aatatgtgtg tagttatttt
     2221 ccttgatatg ttcgatgaga agtggagtaa tttccttata actattgaaa gcttctacag
     2281 gagctcctcc ttcattttta ttagcaaatt tttgcttcca cgaacctaag tgcacctcgt
     2341 agatggatac tggctgttct ttaaaatcat agttaacacg attatacatc cagaggtcat
     2401 ctttgaattt ataacgcgaa ttgtaggttg tagaggcagt gtctggacga acttggctga
     2461 aaaatgcata agggtcagcc ttatatattt cacgcccatc gtgtgtgata atcaggtatt
     2521 tataatcttc aagggatttt aatccgtcta cataaagttg aaatactcca tcaatttcac
     2581 gctccataga gtgactgcgt tgccactcgt taaagttgcc aactaagaat acttctctgg
     2641 catttggggc atagacacga aattgggttc cgataatttt gtttcgttct tttataatat
     2701 gagcacccat tttttcgtaa agagtggtat gctcaccagc attcatataa tataaatcta
     2761 ggtcagtaaa ttctgtcata gcaatctcca ttgccgaaaa ggagtggact agggtcccac
     2821 tccgattcag tatatttttt catcttattt cctaaccact aatttcacgg tagagccaga
     2881 tgtatttctc tgccgaagct gtccaactaa aatcacgctt catagcttgt tcaaccatgg
     2941 cataccagtc atcgggacgg tcatagtagg tttgaagggc taaatcatag acttgtcgca
     3001 tattgtaagc gtcacgccct ccaaagctaa aaccagttcc ttccttggta gacatattgt
     3061 atggtgctac agtatcaacc aagccacctg tttcacgcac tagagggagt gttccgtagt
     3121 gcattgaaat caactggcta atgccgcatg gttcaaacat agaaggcatg aggaagagat
     3181 cgcttgctgc ataaatttgg tgggcaagtg gttcattgta accacggtag aatgcaaatt
     3241 tatcaggata agcgtgttta aagtgattaa aggcattttc aatgtcttcc tcaccattgc
     3301 ctaagataac aaattggaca tgggcttgga gaagatggcc aagtacctcg gtcaagaggt
     3361 agaacccttt ttgccatgtc aaacgagaga cgatacctat caataggaca ttctcatctt
     3421 gtggaagggc aaattgtgct tgcagggcca gtttattagc ctttttactt tcaatcgttt
     3481 caagactata atttctcaca aggtactggt ctgtttgact atcccaactg tcatagtcga
     3541 taccattgac aataccaacc aagtcatatt tgcgcaactc tagtacatgt tgcatgtttt
     3601 cgccgaattc ttccgttagg atttcgcgag cataagtttc agaaactgta gtaaccttgt
     3661 cagcgtatag gataccaagc ttcatgaagc tgatacattc atcatgaaaa cgtgcaatac
     3721 catccaaata gtagctatag tccatgccaa gcgctgacca aagtgcttgt ttgtggaaaa
     3781 taccttgaaa agccaagtta tgaatagtga atacatgctt gatattgcgg aattcttcgc
     3841 ggtagctata gaaagtacgg caaaggaatg gaagggcagc agtgtgccag tcatgtgtgt
     3901 gcatgacatc cgggaagaaa tgaagtgctt ctaacagacg acatgtagca tgttggaaat
     3961 agccaaaacg catggcatca tcgtcatatc catatagttc atcgcgttca aagaaatcgc
     4021 ggtgctcaat aaaatagaat gttactccat caacaacctg actgtaaaca tttgccatag
     4081 attggatgtc gccagcccgc acatcaaaac ttgatacgta atcgaaatca gagtgattgt
     4141 ttactgcaat ttttttgtag agtggtagaa cgactcggac atctaaaccc tgttttacca
     4201 attcttttgg taaagagcca ataacatcag ccaatccgcc agtcttgatg aatggaagtc
     4261 cctcggatgc tacaaagaga acagattttt ttgtcatgtt agctcctaaa tgatttggtg
     4321 tggtttcaag tatgaaggcg aagtttcgct tcccttgatg tcaattgggt gaataatttt
     4381 agttgcttta tcgataattg cattttcaat attcgcccca ctcttgattg tcacgccatt
     4441 gagaatgatt gagtttttga taacaacacc ttcttcaatg ataacatcgc ggtcaatgac
     4501 tgaattttcg acttgaccat tgatattcgc tccatttgaa attaggctac gtttgacctc
     4561 tgccaatgga ccatacaaac atggtgagct atcacttgtt tgggtatgga ttggccaatc
     4621 atgtttgaag aggagtttgc gggtatcttg gtcaatcaat tcaatttgag tattgaagta
     4681 ggaagcggtc gtattgatgc atgcgacata atcacgatgt gcatagccca taatcttgta
     4741 ctggtcaact gtatcacgta aaatatcttt taaccagtag agagacgaca ctttgttagc
     4801 acgtttaatc aagtcgataa atgtagtacg tttcataatg taggcttcaa gggaaacggg
     4861 acggttttta tatttaccgt ggttttcttc aaaagctacg acacgatggt cttcaccaaa
     4921 tttgagagtt tggcagccga tgaaattttc tttagcatct gaaacgtttt tataaagaac
     4981 ggtaatatca gcatccgttg cgatatggtc atccatgact tttgaaaaat cctggctata
     5041 gatgaagtaa gatggagcaa ctagcacata atcttttgta gaattttcga tgtagtggat
     5101 gttttctgtg aaggcattaa tatcgtgacg atagacacta tttgggtctg ttgcgctatt
     5161 gagcaagcga agggtaccgc gtttgctgtt gatattatag tgtttccctg taccaacgtg
     5221 ctgaattgtt gaacgcattt cggcaggcat gtatacttgg aattctgtaa ttccagagtt
     5281 tgacatattg gatagagcaa agtcaattag gcgataacga cctaagaaac cgaaagcttg
     5341 tacgccacgg tgcttcatca cgtcaccaaa atgtacgttg tttccttcaa tatttacaat
     5401 tccgagagta tttcttagca tagcagtcct ttcttacttg gtcacattct tgtcaatcaa
     5461 gaggatatgg tcgatgtctc ctgtgagttt aactccgtcg gcaattttaa tattttcagc
     5521 gacgatgacg tagtcaagtt caacaccttc gccgataata gcaccaggta ggatgacaga
     5581 attttttact acggcatttt ggttgacttg cacatctgtt gaaataacag aatgttctac
     5641 agtaccatcg atgattgccc ctttatcaat ataggcagat ttaaccgttg cagttgcacc
     5701 gatcacctga gcaggtgagc ccttgtcttc tgaatagatt ctccatgagc ggtcagaaag
     5761 gtccaaatct ccagcgtgat caatcaagtc catattggat tcccagaggc tgttaacggt
     5821 acctacgtct ttccaataac cacggaatgg atgggcaatg agagtgcggc catcttctaa
     5881 gtatttcgga atgatgtcgt gcccaaaatc gtgagatgaa gtgtccaatt tgtcatcttc
     5941 ttgaaggtac tttttcaagg tcttccaagt gaagatatag atacccattg atgctaaatt
     6001 gctcttagga ttttctggtt tttcttcaaa ttcttcaata cggtaatctt cgtccgtatt
     6061 catgatacca aaacgtgaag cttctttgat cggaacttcg ataaccgcaa tcgtggcatc
     6121 tgcttttttc tgaatatgtg tatcaaggag tttgtcgtag ttcatcttat agatgtggtc
     6181 accagacaaa atcaatacgt actctggatc gtgaagatca ataaaatcaa tgttctgtgt
     6241 aatagcgtcg gctgtacctt tgtaaagtcc gaaaccttcg attttttcac tcggtggtaa
     6301 tacaaagacg ccagatcctt gtacatcaag cccccaacgt tgagactgtg caacgtatga
     6361 gttcaagaga acaggctcat actgagtcaa aactccgaca atatcaatac ctgagttggc
     6421 acagttactg agaggaaagt caataatgcg gtactttccg ccaaatgcga ccgctggttt
     6481 ggcgactttt tttgtcaacc cttctagacg tgttccacgt ccaccagcaa gaatcatagc
     6541 taacatttta ttttgagcca tctaaccac
//
LOCUS       DBNFEG010000122         6541 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.122, whole genome shotgun sequence.
ACCESSION   DBNFEG010000122 DBNFEG010000000
VERSION     DBNFEG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.122"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(35..586)
                     /locus_tag="AC1PNG_001593"
     CDS             complement(35..586)
                     /locus_tag="AC1PNG_001593"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002268947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIP6702090.1"
                     /translation="MTKALISIDYTIDFVADEGKLTAGKSAQAISERIAQVTQEAFEN
                     GDYIFFAIDGHEEGDEFHSEAQLFPSHNIIGTQGRDLYGPLADFYQKHKGHARVRWMD
                     KRHYSAFSGTDLDVRLRERGVDTVVLTGVLSDICVLHTAIDAYNKGYRIEVVSSAIAA
                     LTEENHQFALNHLRHVLGATIID"
     gene            complement(588..1376)
                     /gene="codY"
                     /locus_tag="AC1PNG_001594"
     CDS             complement(588..1376)
                     /gene="codY"
                     /locus_tag="AC1PNG_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HIP6702091.1"
                     /translation="MTTLLEKTRNITSILKRSEEQLAEELPYNAIAEHLSAIIDCNSC
                     IINSEGEVLGYHMSYETNNDRVEEFFQNKQFPEGYVKAVAQVYDTQVNLPVESELTAI
                     PVESRSTYPNGLTTIAPIHVTGIRFGSLIIWRNDEQFHDDDLILVEIAATVVGIQLLN
                     FQREEDEKNIRRRAAVNMAVNTLSYSEMKAVAAILGELAGNEGQLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLIPAIFDEIKKRDY"
     gene            complement(1847..3061)
                     /locus_tag="AC1PNG_001595"
     CDS             complement(1847..3061)
                     /locus_tag="AC1PNG_001595"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIP6702092.1"
                     /translation="MKQFNKSTKLDDVAYDIRGPVLEEAMRMRANGEQILRLNTGNPA
                     EFGFTAPDEVIRDLIHNARKSEGYSNSKGIFSARKAIMQYCQLKKFPNVDIEDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGKAVHYVCDEAAEWYPDLAD
                     MESKVTSRTKAIVLINPNNPTGALYPKEILKGIIDIARRHELIIFSDEIYDRMVFDGA
                     VHIPIATLAPDLFVVTMNGLSKSHRICGFRVGWMVLSGPKKHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGYQSVDELLMPGGRLYEQREFITKAINDIPGLSAVKPKAGLYV
                     FPKIDREMYRVEDDEQFVLDFLKQEKVLLVHGRGFNWKDPDHFRIVYLPRVDELAEIQ
                     EKMSRFLRQYRR"
     gene            3216..3668
                     /locus_tag="AC1PNG_001596"
     CDS             3216..3668
                     /locus_tag="AC1PNG_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0050896 - response to stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIP6702093.1"
                     /translation="MTQSYKTILVAVDGSKGAELALHKAIHVALRNQARLIIAHVIDT
                     RALHNVVAFDASVYESLEREAELLLEEYKQEALNAGLTDVQICIEFGNPKTLLAIDIP
                     KETGADLMLLGATGLNAFERLLIGSSSEYIMRHATIDLLIVRDGEKSL"
     gene            complement(3684..5049)
                     /locus_tag="AC1PNG_001597"
                     /pseudo
     CDS             complement(3684..5049)
                     /locus_tag="AC1PNG_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503151.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
     gene            5119..6078
                     /locus_tag="AC1PNG_001598"
     CDS             5119..6078
                     /locus_tag="AC1PNG_001598"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIP6702094.1"
                     /translation="MKKILVLHTGGTISMQANHQGEVASSKINPMTQIDSPLEEIQVT
                     SLDFLNVPSPHIRLEHMMTLYQKIKEEQMNFDGFVITHGTDTLEETAYFLDTMAIPEK
                     PIVLTGAMRSSNELGSDGIYNYRTALRVAADDKSADKGVLVVMNDEIHAAKYVTKTHT
                     TNVSTFQTPTHGPLGLVTKREILYFKTAEPRVRFDLSTVTGTVPIIKAYADMDSILLD
                     SLTASSISGLVIEALGAGNLPPTVLPAIKKLLDQKIPIILVSRCFNGIAEPVYAYQGG
                     GIQLEQDGILFVKELNAQKARLKLLIALNAGLEEQSLADYIQG"
     gene            complement(6136..6541)
                     /locus_tag="AC1PNG_001599"
                     /pseudo
     CDS             complement(6136..6541)
                     /locus_tag="AC1PNG_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943028.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2059 a         1541 c         1088 g         1853 t
ORIGIN      
        1 ccagtcgagt tctttcccgc tccccactat actattagtc aataattgtc gcaccgagta
       61 catgacgcaa atggttaaga gcaaactgat gattctcctc cgtcaatgca gcaatggctg
      121 atgagaccac ctcaatacga taccccttat tataagcatc aatagctgta tgaagaacac
      181 aaatatcaga caaaacaccc gtcaagacaa ccgtatctac accacgctct cttagtcgaa
      241 catccaaatc cgtcccagaa aaagcagaat aatgacgttt atccatccaa cgaacacgcg
      301 catgcccttt atgtttttga taaaaatctg ctaaaggacc atacaaatca cgtccttgcg
      361 tcccaatgat attatgactt gggaaaagct gagcttcaga atgaaattca tccccctcct
      421 catgaccatc aatcgcaaag aaaatatagt ctccattttc gaaagcttct tgcgtaacct
      481 gagcaattcg ttcagaaata gcctgagctg attttccagc agtcaacttt ccttcatctg
      541 ccacaaaatc tattgtataa tcaatcgaaa tcaatgcttt tgtcatttta gtagtcacgt
      601 ttcttaattt catcaaaaat agctgggatg agaactttca aataagtccc cttcattccc
      661 aaagaacgac tttcaataat ccctgcactc tccaacttac gcagtgcatt cacaatcacc
      721 gagcgtgtaa taccgatacg atctgcaatc acagaagcag tcaattgccc ctcattgcca
      781 gccaattcac ccaaaatagc tgcaactgct ttcatttctg agtaagatag tgtatttacc
      841 gccatattaa ctgccgcacg acgacggata ttcttctcgt cttcttcccg ttggaaatta
      901 agtaactgaa taccaactac tgttgccgca atctcaacca aaatcaaatc atcatcgtga
      961 aactgctcat cattccgcca aataataagc gaaccaaaac gaatccccgt tacgtggata
     1021 ggcgctatcg ttgtcagccc gtttggataa gtcgagcgtg attcgacagg gatggcagtc
     1081 aactcgctct cgacaggcaa attaacctgc gtatcgtaaa cctgcgcaac tgcttttaca
     1141 tatccttctg ggaattgttt gttttggaaa aattcttcca cacgatcatt gtttgtctca
     1201 taactcatgt ggtatcccaa aacttcacct tcgctattaa taatacaaga attgcaatca
     1261 ataatagctg atagatgttc agcaatagca ttgtaaggca attcttctgc caattgctct
     1321 tcggaacgct tcaaaataga agtaatattc cgtgtcttct ctaataatgt tgtcatattt
     1381 tcctctttac tctgtaattg ctttcagtat agcaaaaaat acataaaatt tcaacaatta
     1441 tcagaatatt taccgttgtt agaaaaacac aaacaatttc atccaagcaa aaagaaatag
     1501 gctgggcaaa aagcccagca ccgcttctca aagttagtgt caacatctca gcgcagtggt
     1561 tgattgggtt taacagtcca gtggactgtt aaaggttgga gataagattt gcgaagcaaa
     1621 tctcagcaat tcgcatttta cactccaaat ctggcctata cgactgatgc gaacagagtt
     1681 cgcttcatct ccaccctcaa actgtctccc agacagtttg agctgtgcgg gggtgggagt
     1741 gaaacagtct gggaatagac tgtttcagct caacaactag aaataaagac ttgttgacga
     1801 actcttctta tgaatggttg agttctttcc cactcccaaa attccattat ctacgatatt
     1861 gccgcaagaa tcgtgacatt ttttcttgaa tttctgccaa ctcatcaaca cgtggtaaat
     1921 acacaatacg gaaatgatct ggatccttcc agttaaaacc tcgaccatgg accaaaagta
     1981 ctttttcttg cttgaggaaa tccaatacaa attgttcatc atcctcaact ctgtacatct
     2041 cacgatcaat ttttgggaat acatacagac cagccttagg ttttacagca gaaagtcccg
     2101 gaatatcatt aatcgccttg gtaatgaact ccctttgttc atataagcgt ccgccaggca
     2161 tcaagagctc atcaaccgat tggtaacctc ccaaagaagt ttgaacaacc tgttgagcca
     2221 aaacgtttga acacaaacgc atattggaca acatattcaa gccttcaatg taacctttta
     2281 catgcttttt cggaccagat aagaccatcc aacctactcg gaaaccacaa atacgatgtg
     2341 atttagacag tccattcatc gtcacaacaa ataaatctgg agccagtgtc gcaataggga
     2401 tatgaacagc tccatcaaag accatacgat cataaatctc atcagagaaa atgatcaatt
     2461 catgtctacg agcaatatca atgattcctt ttaaaatttc tttcggatag agagcaccag
     2521 ttggattatt tggattgata aggacaattg ccttggttcg agaagtcacc ttggactcca
     2581 tatctgccaa atctggatac cattcagctg cttcatcaca aacataatga acagctttac
     2641 caccagccaa actaactgcg gcagtccaga gaggatagtc tggcattggc accaatacct
     2701 catcaccatt atctagcaaa ccttgcatag acataacaat cagctcactc acgccgtttc
     2761 caagataaat atcttcaatg tcaacatttg gaaatttctt tagctgacaa tactgcatga
     2821 tagccttacg tgcagaaaaa attcccttac tgttcgaata tccctctgat ttacgggcgt
     2881 tatgaatcaa gtcacgaata acttcatctg gagcagtaaa accaaattct gcaggattcc
     2941 ccgtattcaa acgtaaaatc tgctctccat tggcacgcat gcgcatggcc tcttccaaaa
     3001 caggcccacg aatatcatat gctacatcat ccaattttgt tgacttgtta aattgcttca
     3061 tgtcgtcacc tcgactttct atcttttcta ttgtactgta aaagctaaaa aagtacaaga
     3121 atcctttaca ttttctctca aaaggcttat aataaaaatg taaggaacta ctctacctat
     3181 ttcgggacaa agctcttctg aaaggagtga ttattatgac acaatcttac aaaacaatac
     3241 tcgtcgctgt agacggctct aaaggagcag aattagctct tcataaagcc attcatgtcg
     3301 cactacgcaa ccaagcacgt ctgattatcg cccatgtgat tgacacccgt gctctccaca
     3361 atgttgttgc atttgacgcc tctgtctatg aatctttaga aagagaggcg gagctcctcc
     3421 tcgaagaata caaacaagaa gctctcaacg ccggtctgac cgatgtacag atttgcattg
     3481 aattcggtaa ccctaaaacc ctactggcta tcgatattcc aaaagaaacc ggtgctgatc
     3541 ttatgttact tggagcaact ggtctaaacg cctttgaacg tctcctgatc ggatcctcat
     3601 ccgaatatat catgcgccat gcaactattg atttactcat tgttcgcgat ggagaaaaaa
     3661 gtttataaac caaaagactt gatttacgaa tcaagtcttt tcatctgtct tttcagctct
     3721 tcctgaatct tttcctccgg cgcaaaccgt tcttcccaat catcaggttt taaaatttta
     3781 tgggtaattg gatcaaaatg ggccttccca tctggaaaag ttttccccat atttgcagta
     3841 tggactatct gaaaaattgg ttctggatca acccccatca aaacaaacga accatatgta
     3901 aaataaagaa tatccagcaa agcatctacc tgatcctgca tcgaaacccc gaaacctctc
     3961 ttgccagata ccttagcagc agcagtatct aaatcccgat gaagttgttg aataaaatca
     4021 tgaaactcaa cttcagattc tgaagctgca tgtagaaatt ctaccaactc ctccacttta
     4081 aaaccagcgc gatgcaaagc cgtctgtcca tcatacacac tgggaagttc ttgtgtttca
     4141 ccatccatta aaaaatgaaa atcctttacc ctattaaaat tatcgtcctt gctctgaaac
     4201 atcttatccc tcaatcacac caaaatgaat taatgcctta taaatcccat cacgattatt
     4261 tgtatccgta acaaaggaag cagcttgttt tacctttttc gtcccatttc ccatcgctac
     4321 cgagtgctga atttcattca acatttcaaa atcattatta gaatccccga ataccataac
     4381 ttcatctaac tcaaaaccat atcgatctgc aacctttaca atacccgata attttgaatt
     4441 tcccttactg ataatatcag tcgcataggg actcgaacgc gtaaaagaca agttcggaaa
     4501 gagcatctcc aatgattgtg tctccctttc cgtcgtcagc aacatcagct gatagattgg
     4561 ttgaaagaga aaacctttca aattcgtttc ctgttgagga ctaatccaac gtaccaagcg
     4621 attgaaaata aaatttatga ttccagccca ggactccgga atcatacgcg tcacacgata
     4681 ggcaaaattt cctgttccag cactcataat cttactacca aatatgcctt tagcagttcc
     4741 aaaagataaa tccttatggt gagtttgcgc atactccata atttgttcaa tttgctttgg
     4801 ctctaaactc tgactataaa taacttcttc tcttgaaaaa atatattgac cattataagc
     4861 aatggccaaa tctagcccta aactagccat atactgcaaa acaaaacgag ggtcacgtcc
     4921 tgtagctaga ccaactaaaa tccccttctc tttcaaagca ttaatggcga gaatagttga
     4981 actactaacc atacgattgt cggtcaataa ggtaccatca atatcgaaaa atacagcctt
     5041 tatcgtcaac caactcacct actttctaag aattatgtta caattagtat atcacacttt
     5101 gaaagaaact gaaaatttat gaaaaaaatt ctagtactgc atacaggtgg aaccatttct
     5161 atgcaagcca atcatcaggg tgaagttgca tcgagtaaaa tcaatccaat gacccaaatt
     5221 gatagcccac tagaagaaat tcaagtgaca tccttggatt ttctcaatgt tccaagtcca
     5281 cacattcgct tagaacacat gatgactcta taccaaaaaa ttaaagaaga acaaatgaac
     5341 ttcgatggtt ttgtcattac acatggcaca gatactctag aagaaactgc ctatttcctt
     5401 gatacgatgg ctattccaga aaaaccaatt gtattgaccg gagccatgcg ttcctcaaat
     5461 gaactaggta gcgacggaat ttataactac cgaacagccc tacgtgtggc tgctgatgat
     5521 aaatccgcag ataaaggtgt tctagtggtc atgaacgatg aaattcatgc tgctaaatat
     5581 gttacaaaaa cgcataccac caatgtctca acctttcaaa caccaaccca tggaccactg
     5641 ggattagtca ccaaacgaga gattctctat tttaaaactg ctgaaccacg tgttcgattt
     5701 gacttatcaa cagtcacagg aacggtccca atcataaaag cttatgcaga tatggactcc
     5761 attctccttg attctctcac tgcaagctca atttccggtt tagtcattga agcactcggc
     5821 gccggaaatc ttccgcctac cgtccttcca gccattaaga aactccttga tcaaaagata
     5881 cccattattc tggtatctcg ctgttttaac ggaattgctg agcctgtcta tgcctaccaa
     5941 ggaggtggca tccaactgga gcaagacggc attctatttg ttaaagaatt gaatgctcaa
     6001 aaagcccgac tcaaactcct tatcgctctc aatgcaggct tggaagagca aagcttggca
     6061 gattacatcc aaggttaatc gctgacgaac tatacaatat gggctctttg tcaactgtag
     6121 tgggtagatg aaaagctaac acctagagag gacgaacttc gttctctctt tctttatctc
     6181 tttcttgttt taggagataa gctgaaaagg gctcttccca gcccttttca ctgttcaaag
     6241 caatcaaaat gcgtttttta aagttctcaa agttcctgaa accaaaggca tttctcttaa
     6301 tgactttgat gagattatta gtagcttcca gtttggcgtt ggaataaggc aattctaggg
     6361 cattcaaaac cttgtcttta tcctttagca atgtcttaaa taccgtctgg aaaataggat
     6421 taacagttgc tatttcctgt tcaacgaggt caaagaaatg gtctgagttc ttctcttgga
     6481 aatgaaataa gagaagctga tagagttcat agtgctgtcg tagttcctcg gagaaagaca
     6541 g
//
LOCUS       DBNFEG010000123         6406 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.123, whole genome shotgun sequence.
ACCESSION   DBNFEG010000123 DBNFEG010000000
VERSION     DBNFEG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.123"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            51..1841
                     /gene="dnaG"
                     /locus_tag="AC1PNG_001600"
     CDS             51..1841
                     /gene="dnaG"
                     /locus_tag="AC1PNG_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIP6702095.1"
                     /translation="MLSKDKITEIKQALNIVDVIGETVALTKAGRNYVGLCPFHGEKT
                     PSFNVIEDKQFYHCFGCGKSGDVFKFVEEVRGVSFADAAAILAEKAGFQTDVTPSYHQ
                     EEKRVSPHQVLYDIHRDAAKFYHALLMTTKMGEEARAYLHQRGLTDDVIKTFQLGLAP
                     AEQNILYQKLSGQYDEESLLNSGLFNPSENNIIYDAFQARIIFPLADEYGRIVAFSGR
                     IWTEENRNNKQLAKYKNSRSTAIFNKSYELYHLDKAKAVIKKQRETYLMEGFLDVIAA
                     HRVGIDNAVASMGTALTREHVAHLAKFCKKIVLTYDGDKAGQAATMKALDELQDFQVE
                     IVSLPDNMDPDEFLQKNSEEALQQVLTKSRISDVEFLIQYLKPENPDNLQMQIEFVDK
                     IAPIIAKVSSITAQNSYIYKVADLLSDFDYNQVEQAVNAVRLNQRQERTQQVFQSQTG
                     QSYLPPVQTIARITSLIRTENHLLYRMVEHPYILNEFRLREDFYFATPELQAIYDMLK
                     KSGEITSFELSQLNDSAQQAWYRMQEERLPDEVSPHEIEELEIRRDKELLKKENQHLA
                     RKIREHSHVGNADIALDELQKLLDRRRQME"
     gene            1844..2962
                     /gene="rpoD"
                     /locus_tag="AC1PNG_001601"
     CDS             1844..2962
                     /gene="rpoD"
                     /locus_tag="AC1PNG_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIP6702096.1"
                     /translation="MTNKKDKKTEVTTFDVQVAEFIRNHKKQGSATDDEINDQLVIPF
                     TLDADGIDDLLQRIQDAGISIVDKEGNPSARAMQVEEEPELSDEELLGSTSAKVNDPV
                     RMYLKEIGVVPLLTNEEEQELALAVEAGDPEAKQRLAEANLRLVVSIAKRYVGRGMQF
                     LDLIQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVET
                     INKLVREQRNLLQELGQDPTPEQIAERMDMTPEKVREILKIAQEPVSLETPIGEEDDS
                     HLGDFIEDEIIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLED
                     VGKVFNVTRERIRQIEAKALRKLRHPSRSKPLRDFIED"
     gene            3347..3670
                     /locus_tag="AC1PNG_001602"
     CDS             3347..3670
                     /locus_tag="AC1PNG_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIP6702097.1"
                     /translation="MTYTEDQIKDIQERIFHALEDVIDPELGIDIINLGLIYEIRFIE
                     GKAEIDMTLTTMGCPLADLITDQIHDVLKDVPEVTEVDVRLVWSPAWTVQKMSRYARI
                     ALGIK"
     gene            3851..4008
                     /locus_tag="AC1PNG_001603"
                     /pseudo
     CDS             3851..4008
                     /locus_tag="AC1PNG_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775157.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4095..5225)
                     /locus_tag="AC1PNG_001604"
                     /pseudo
     CDS             complement(4095..5225)
                     /locus_tag="AC1PNG_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            5321..5644
                     /gene="tnpA"
                     /locus_tag="AC1PNG_001605"
                     /pseudo
     CDS             5321..5644
                     /gene="tnpA"
                     /locus_tag="AC1PNG_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373723.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5784..6377)
                     /locus_tag="AC1PNG_001606"
     CDS             complement(5784..6377)
                     /locus_tag="AC1PNG_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIP6702098.1"
                     /translation="MYKFWQKTIQVLSILTLIGTAIFLFWLYKIGILNDQNVLSDLIK
                     SQGALGSLSFLAIQIIQVVFPIIPGGVTTVVGFLVFHFWWGFFLNYLGISIGSIILFW
                     LARRYGKKFCLLFMSEDTFYKYESKIDNKRSYEIFFILCMLSPISPADIVVMITGLTS
                     MSYRKFIMITLLCRPFSVVAYSFFWIYGSQWLQQLLG"
BASE COUNT         2014 a         1238 c         1335 g         1819 t
ORIGIN      
        1 ggggagagtg ttcacttttt aaattagcta gtatgatatg aggagggaat gtgctttcaa
       61 aggataaaat tactgaaata aaacaagctc tcaatatcgt tgatgttatt ggtgaaacgg
      121 ttgctttgac taaggccgga cgtaattatg tcggtctttg cccttttcat ggggagaaaa
      181 ccccatcttt caatgtgatt gaagataagc agttctacca ttgttttgga tgtggtaagt
      241 ccggagatgt attcaagttt gtcgaagaag ttcgtggtgt ttcttttgca gatgcggcag
      301 ctatcttagc tgagaaggca gggtttcaaa cagatgtaac tccttcctat catcaagagg
      361 agaaaagggt tagtccgcat caggtactgt atgatattca tcgggatgct gcaaagtttt
      421 atcatgccct tttgatgaca accaaaatgg gtgaggaagc tagagcttat ctacaccaac
      481 gtggcttgac agatgatgtc ataaaaacat tccagctagg cttagctcct gccgaacaga
      541 atattcttta ccaaaaacta tcaggtcaat atgacgaaga aagtctattg aattcgggtt
      601 tgttcaatcc aagtgagaat aatattattt atgacgcctt tcaagcgagg attattttcc
      661 ccttggcgga tgaatatggt cgaattgtag ctttttcagg tcggatttgg accgaagaaa
      721 atcgaaacaa taaacaatta gctaagtata aaaactctcg tagtactgcg attttcaata
      781 aaagctatga attgtatcat ttggacaagg ctaaagcggt tattaagaag caacgtgaaa
      841 cctatctcat ggaaggtttt ctagatgtta ttgctgctca tcgtgtaggg attgataatg
      901 cagtggcttc catgggaaca gctttgacaa gagagcatgt tgcacaccta gctaagtttt
      961 gtaagaaaat tgttctgact tatgatgggg ataaggctgg acaggctgct accatgaaag
     1021 cgttggacga actacaagat tttcaggttg aaatcgtgag cttgccagat aatatggacc
     1081 cagatgaatt tttgcagaaa aattcggaag aagccttgca acaggtttta accaagtcac
     1141 gaatcagcga tgtagaattt ttgattcaat acttgaaacc tgaaaatcct gacaatctac
     1201 aaatgcagat tgagtttgta gataagattg caccaattat tgccaaagta tcatccatta
     1261 cagctcaaaa ttcttatatt tataaggtgg cggatttact ttctgacttt gattataatc
     1321 aggtggaaca ggcagtaaat gctgttcgtc tgaaccagcg ccaggagaga acacaacagg
     1381 tatttcaatc acagactggt cagtcttatt tgccaccagt ccaaacgatt gctcgtataa
     1441 cgagtcttat tcgaacagaa aaccatttac tttatcggat ggttgaacat ccttatattc
     1501 tcaatgagtt ccgtttacga gaagattttt attttgcgac gccagaattg caagctattt
     1561 atgatatgtt aaaaaaatct ggggaaatta ccagttttga actatcacag ttgaacgatt
     1621 ccgctcaaca ggcttggtat cgtatgcagg aagaacgttt acctgatgaa gtgtctccac
     1681 acgaaatcga agaattagaa attcggcgag acaaggaatt gttaaagaag gagaaccagc
     1741 acctagcacg gaaaatccgt gaacattccc atgtgggaaa tgcagatatt gccctcgatg
     1801 agttgcaaaa actcctcgat agaagaagac aaatggagta aatatgacaa ataaaaaaga
     1861 taaaaaaaca gaagtaacta cttttgatgt acaagttgcc gaatttatcc gtaaccataa
     1921 aaaacaaggt tcagctacag acgatgaaat caatgaccaa ctggttattc catttacgct
     1981 tgatgctgac ggaatcgatg atttgctcca acgtattcag gacgcaggga tttcgattgt
     2041 cgataaagaa gggaaccctt ctgctcgtgc tatgcaggta gaggaagaac cagaattatc
     2101 tgatgaagaa ttgctcggaa gcacttctgc aaaagtgaac gatccagtcc gcatgtattt
     2161 gaaagaaatt ggtgttgtac ctcttttgac caacgaggaa gagcaagaat tggcattggc
     2221 ggtcgaagca ggtgacccag aagcgaaaca acgtttggct gaagccaact tgcgtttggt
     2281 tgtatccatt gccaaacgct atgtcggtcg tggtatgcag ttccttgact tgattcaaga
     2341 aggaaacatg ggcttgatga aggccgttga taagtttgac tactctaaag gtttcaaatt
     2401 ttcaacttat gcaacctggt ggattcgtca ggctatcact cgtgccattg cggaccaagc
     2461 gcgtacaatc cgtatccctg ttcacatggt agaaaccatt aacaaattgg tccgtgaaca
     2521 acgcaatctc ttgcaggaat tgggccaaga tccaactccc gagcaaatcg ctgagcgtat
     2581 ggatatgacg cctgagaaag ttcgtgaaat cctcaagatt gcacaagagc ctgtctcgct
     2641 cgagacccca attggtgaag aagatgatag tcatcttgga gattttatcg aagacgagat
     2701 cattgaaaat ccagttgatt acacaactcg tgtcgttctt cgtgaacagt tagatgaggt
     2761 attggatact cttactgacc gtgaggaaaa cgtattgcgt cttcgttttg gtttagatga
     2821 tggtaaaatg cgcacgcttg aagatgttgg caaagtcttt aacgtaaccc gtgaacgcat
     2881 ccgccaaatc gaagccaaag ccctccgtaa actccgccac ccatcccgca gcaaaccact
     2941 cagagatttt attgaggatt agaaaggtac tgcgtacctt tctaacgcct gcccagaaat
     3001 aagaaagcag gctaacccag tggtgtttta acccgagcct agaaatagga aagcgaggct
     3061 aatctccggg ggaggtgatc gacgtttctc atatcattcg accataatga tgtgagaaac
     3121 tagtaagtcg tctaggcaat tctattggga attgccgtta gttgagtagc ttttcaaagc
     3181 actcaacttt ggcgagttga accgctcgct gaaaacagga aacgagcgag attttaaccg
     3241 ctccccttga aattagaaag ggagcgtaac gtccagtgga ctttttgaca caaacttcgt
     3301 ttgtttcttt tctagtattg aacttgtgaa aaagaaagag agaaaaatga cgtacacaga
     3361 agatcaaatt aaagacattc aggagcgtat ttttcatgct cttgaagatg tgattgaccc
     3421 tgagctaggt attgatatta tcaatttagg gctaatttat gaaatccgat ttattgaggg
     3481 caaggctgaa attgatatga ccttgacgac catgggttgt ccattagctg atctaattac
     3541 cgatcaaatt catgatgtat tgaaggatgt ccctgaagta acagaagtag atgtgagact
     3601 ggtttggtca ccagcttgga ccgttcaaaa aatgagtcgt tatgctcgaa ttgcattagg
     3661 aattaaataa aaaaacaaaa tggtttcgaa cgtttgaagc cgttttttta ttatggcttc
     3721 gagccagttt ttctcgtaga gaaaaacaag aaagccacca gctatgctgg tggctgccaa
     3781 ggctttgagt ttccatttct ttttcctgtt ataatctaat tgttcaggtt agatcaagga
     3841 ggaaaaagaa atggctaaaa ccagttacag tttatcacat actaaatgga tgtgctctat
     3901 cacatagttt tcacacctaa gtaccgtaga aaatccattt actacaaaat aaggcaggac
     3961 ttaattgata ttttccgtca tctatgtcga tgcaaaggcg tggacatcac gtcatacgca
     4021 tgaaaaattt cttctctcct tttgtccccg gaaataactg ctttaaactt agtatctacc
     4081 tttttacaag caccttatcc ccgatttcca aataattgtg gcaaataatc ctccaaatgg
     4141 acagaaatat agcgttgttt gcggcggtaa ctcaatttct ccttagggaa ggtcatcatt
     4201 tgtaagagat gaagacaagc cttacgaata gcatttaaat tgaaagcagc tcgtttatct
     4261 aaggtttggt tcttatcttc tcggtaaacc acatctaaca accaatgtag gctctcaacc
     4321 gaccaatgac cacgaacaac ctgagaaaaa gtttgcacat cgcccttgaa actaaggata
     4381 aagtaacgga cctcttcagt gatgacacca tctttatcaa tgatattctt agtcatacca
     4441 atcccacgaa gttttttcca tttcggatgt cgttggctca gccatttcac atcgtgagaa
     4501 acccagtagt ccctcacctc aatctgactg cgtgcctttt caatggtctg ataatgacag
     4561 cctttctctg tcaacgtaga taacaatttg gcgtcactaa aatagaggtc aatatcctca
     4621 tggagattgc cttgatttcc tttgactgcc aagcaataat cacccttacc gctgataatc
     4681 acatccgcaa tgtctgtcta ggtgcccatg gcatcaatcg tgacaacact cttacgaagg
     4741 tctagctgac acaagaggcg aggaatggct gtgatttcat tacttttatc ctcaacagct
     4801 acctgcccaa ggctaatccg attaccacca tcataagcag tgacaatatg agtaggagat
     4861 tggtgtttcc ctcggttccc ccgaatcgtt tttccgtcaa cggcaatcaa ttttgaaaaa
     4921 tcagttgttt ccgaactagc ttcgaaggac agtttcagtt cctttaagaa atctggattg
     4981 accatactca caacacgttc caaggtgtca tgagaaggac aaccagcact caagtcaacg
     5041 tattctctca agacagattc gttcatttct atgaaatctt ccatctcttt ccacgtttca
     5101 acccctgcta attgacaagc gaaaaccaga aacagaatgg tagatagagg atatcggatt
     5161 ttccatgctt gacggctatc atactcttct ttgtgctctt ctatggataa aagaaaatca
     5221 atcaaagtag ttcacctcta ttctagtgta ctactttgat tgatatttgg ttatgatttc
     5281 cgctttctga aaacgatttt ttcttcatgc gtttggcgtg gtggaaatca ttgagggaca
     5341 catgatgcca gaccatgttc atatgcttgt cttgatatcc ccaaaacttt cgatttccga
     5401 ttttatggga tatttgaaaa gtaaaagtgc tctcatgata tttgataaac atgctaattt
     5461 aaaatataag tatggaaatc gaaagttttg ggctagaggc tactatgtga gtatcgttgg
     5521 actgaacgaa aagacggtag cgaaatgtat tcgcgagcag gagaaaaatg acatcgcact
     5581 tgataagtta agtgttaaag aatatgaaga ttcattttca gatagtagtt ttagaacaag
     5641 ataaaagccc gttgttacgg gctttagtca agtaattata gcactaacct gaacgaagtt
     5701 cagcgaacgt ctttagacgt cgctggagag aaacggctta tagccggtgt acaagccacc
     5761 cgttttcacg ggtggttatg attttatcct aacaattgct gcaaccattg gctgccatag
     5821 atccaaaaga agctataggc cacaactgag aaagggcgac aaagtaaagt aatcataata
     5881 aatttccgat agctcatgct tgttaatcca gtaatcatca ccactatgtc agctggagaa
     5941 atgggagaaa gcatgcaaag aataaagaaa atttcataac tacgtttatt atcaattttg
     6001 ctttcatatt tgtaaaacgt atcttcagac atgaagagga gacaaaattt ctttccatag
     6061 cgacgagcca accagaaaag gatgatacta ccgattgaaa ttccaagata attgagaaag
     6121 aatccccacc aaaaatgaaa tacgaggaaa cccacaacag tcgtaacacc gcctggaata
     6181 atcggaaata caacttgaat aatctgaata gctaaaaagg agaggctacc taaagcacct
     6241 tggcttttga ttaaatcgct taagacattt tggtcgttca aaatgccaat cttatagagc
     6301 caaaacagaa aaatagcagt cccgataagg gtcaaaatac ttaatacttg aatggttttt
     6361 tgccaaaatt tatacatact ttcattttac catatttcaa aatttt
//
LOCUS       DBNFEG010000124         6313 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.124, whole genome shotgun sequence.
ACCESSION   DBNFEG010000124 DBNFEG010000000
VERSION     DBNFEG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.124"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..2245
                     /locus_tag="AC1PNG_001607"
     CDS             <1..2245
                     /locus_tag="AC1PNG_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HIP6702099.1"
                     /translation="YENGIFQLTDGYYTKMIAFEDINYQLALEEQRDFIFNQFAAFLN
                     SFDNSTRLQLCFTNQLGRLDELADAITIPSKDDGLEAIRCEFRVMLQNQLEKGNNGLR
                     QTKYIVFGVKEDTYRQAKVVLSRLEIDLQSHLKGMGIRSIVLNGFERLIVLHHILNPT
                     EQVSELPEDLGNSDCRSSIVPDKFHFQKKYFSMDNVLCQTNHVQILASELSDRFLTEL
                     LSLEENMVLSLHIQALDQVEAIKLVKRKFTDVQRMTMEEQKKAYRSGYDLDIIPSDLM
                     TYGTDLKHLLNDLQSRDEKMFLVSFILLNQGLTKAKLDNAVSQLATICSRHNCMLKCL
                     DYQQEQGLISALPLGMNCIKIKRQLTTSSTAVFIPFTTEELFMTSSTSIYYGLNAISH
                     NLIMADRKKLKNPNGLILGTPGSGKSFSAKREISNAILVTDDDILICDPEGEYGALVE
                     AFGGEVIHVSAKSKDFLNPLDINFNYGDGDAPLKDKANFIMSMLELVVGGSGLTAAEK
                     SVIDRCLPKIYEAYFENPIPENMPILQDLYDLLLKQEESVGRKLATEMEIYVTGSLNV
                     FNHRSNVDLNKSLLCFDIKELGTQLKKIGMLVIQDQVWNKVSQNRQSQKSTRYYIDEF
                     HLLLKEEQTAQYSVEIWKRFRKWGGIPTGLTQNVKDLLASKEIENVFDNTDFIMMLNQ
                     ASGDREILARKLKISPYQLNYVTNSNAGEGLLFFGNTIVPFLDQFPKNTLLYQKMTTK
                     PEEVRKG"
     gene            2253..5474
                     /locus_tag="AC1PNG_001608"
     CDS             2253..5474
                     /locus_tag="AC1PNG_001608"
                     /inference="COORDINATES: protein motif:HMM:NF045974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HIP6702100.1"
                     /translation="MGKSKRMVRETNKKHYTKSKPDISQRKEEQDFSGRTVDESVKFQ
                     HQIIHKESKLESRKNQVESSGKRRKNINIVDDKSTQTPLPIHDKNESTIQITDLQDEI
                     VNKNYLHQPSPPEAPLVESKPISHKKQVQLQQRKAFENQQNVESRNDIEFTEQNGHNS
                     EIHVSSDGDLAQTGNEQEVQQIQFEIVHHNPVNSSELNHSKELNFGPTSSQTQAQNNS
                     QPTESIDRKIHDTPFRKFDTNQSRIIDTETKFTHRRNEGRAKLIHNQNQGTPSQFKNK
                     KGKKHVHGDSVHESQNILSELPISQFDSSTDSVPIKTYPRVSDESNSIIDQADNESVF
                     DVSLLQSQLRKDNKKFQPSNSKLSKKSGVKIIEAKNSQSQQIEKTVTLTDGGKQSSNQ
                     FQSNLNENSIMIEQISYPNIDWSKKLHLPQIKLFSRDILHSNAVSGKIQNDESKPLTR
                     KGKRLSNRLSNKATKSNIARSLYPDTTSDKNRKTVSKSSKTKLSITPIQVDGRPKYYF
                     ETTTKKQFYKGRPSSTNNIKANKFRRQRLLKDQLQIHEKLNSLVIEPIEFVKDKKNRR
                     LLKKIEQAKLGRIKSSPLKKVVSGLASSEFLAASYLQAGADDNVAVDATSKVLNINAA
                     ILMRLAKKSNRNQTIKKLTKKAKVRQSQLEFRHKYRLMKEDKIFQRQSFYRKSMYRQR
                     MKQQIRNKHFPRIRDRIKEELKKLLNQMVQFITTKWKSILVLALALFSLLTVVYSNSQ
                     FMFGALSSVSYQLTTTSYLSSEEMLVALNHIFSTYELNLSNQLARLKESKPGYDEYIV
                     KGREQIGHDPHVLLAYLTARFGEIKDQTSVEPAMKQLFVQIYQVSYREEREIRYRKVK
                     EEVLDTSGKKTIVEKEVPYTYRKLIATVSKNDMDVVIQSIFAGSPSNLEHYRILRETK
                     GNLETAFPSGTTISLPGNVSSVYDVNLTGGNFPPPNPNHVAALNGGYPGQCTWYVYNR
                     FSQLGKPIQHSPMGNGGEWAFYAAKYGYPVSREARAGTAVCMPPTVPYADPTYGHIAF
                     VERVNPDGSIVISEMNVKGEFVISTGYLPREMAAQCYYINFGL"
     gene            5485..5700
                     /locus_tag="AC1PNG_001609"
     CDS             5485..5700
                     /locus_tag="AC1PNG_001609"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702101.1"
                     /translation="MRKDIQMLQKKKEKLLEKKQKIDQDLAQVLQEEQDLIYEEVIVV
                     FEKSNLSLEEFTKKVLFEKELSHETII"
     gene            5684..6280
                     /locus_tag="AC1PNG_001610"
     CDS             5684..6280
                     /locus_tag="AC1PNG_001610"
                     /inference="COORDINATES: protein motif:HMM:NF025641.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HIP6702102.1"
                     /translation="MKRLFSFVRVASLVMLFSGCQTIYASSPSSEPNSQPVAISVHPQ
                     ASEQSNAKGQVTENVDANNQDYEITDSVATKRQFVTFTSKSGKVFHLIIDHDKGGQNV
                     QLLTEVSEQDLLNLVESTDTVAVKPQKVEEVVDDKPVKNEEPKQKSSAGSYIIIGLFL
                     LGVLCVGYYIKVIKPKKEHHFEEFEEDDDYISDGEEEV"
BASE COUNT         2267 a         1016 c         1220 g         1810 t
ORIGIN      
        1 atatgaaaat ggaatttttc aattaactga tggttactat acaaaaatga ttgcctttga
       61 agatattaac tatcaattag cattggaaga gcaacgagat ttcattttca atcagtttgc
      121 ggcattttta aatagttttg ataattcgac cagattgcaa ctttgtttta ctaaccaact
      181 tggaagattg gatgaattag cagatgcaat tacaatccca tctaaagatg atggattaga
      241 ggctattcgt tgcgaattta gagttatgct tcaaaatcaa ttggagaagg ggaataacgg
      301 cttaaggcaa acaaaatata ttgtttttgg tgtaaaagaa gatacttatc gtcaagcaaa
      361 ggttgtttta tccaggttgg aaattgatct acaatctcat ttgaaaggga tggggattcg
      421 ttctattgtc ctaaatggtt ttgaacgatt gattgtactt catcatattt tgaacccgac
      481 agagcaagtt tctgaattgc cagaggattt gggcaatagt gactgtcgct cttcgattgt
      541 gccagataaa tttcactttc agaagaagta tttttcaatg gataatgttt tgtgtcagac
      601 caatcatgtg cagattttag catcagaatt atcggatagg tttttaactg aattactttc
      661 tcttgaggag aatatggttc tatcgctaca tatacaagca ttagatcaag ttgaagcgat
      721 caagctagtt aaacgtaagt ttaccgatgt gcaacggatg acgatggagg agcagaaaaa
      781 ggcctatcgt tctggttatg atttagatat tattccttcc gatttgatga cctatggtac
      841 tgatttgaaa cacctattga atgatttgca gagtcgtgat gaaaagatgt ttctcgtatc
      901 gtttatcctt cttaatcaag gattaacaaa agcaaaactg gataatgcag tctctcaatt
      961 agcaactatt tgtagccgac ataattgtat gctgaaatgt ctggattacc aacaggagca
     1021 aggattaata tcagctttac cgttaggaat gaattgtatc aaaattaagc ggcaattaac
     1081 gacttcaagt acggccgtct tcataccctt tacgactgaa gagcttttta tgacttcaag
     1141 tacatctatc tattatggat taaatgctat aagtcataac ttgattatgg cagataggaa
     1201 gaaactaaag aaccccaatg gcttaatctt agggacacca ggttctggaa aatctttctc
     1261 agccaagcgt gaaatttcca atgccatact agtaacagac gatgatattc ttatctgtga
     1321 tccagaagga gagtatggtg ctcttgtaga agcatttggt ggagaggtta tccatgtttc
     1381 cgctaaatct aaggattttt tgaatccatt agatataaac ttcaactatg gagatggcga
     1441 tgcacctctg aaagataagg cgaattttat catgtcaatg ctcgaacttg tagtaggcgg
     1501 ttctgggtta acagccgctg aaaaatctgt cattgaccga tgtctaccga aaatctacga
     1561 agcatacttc gaaaacccaa ttccagagaa tatgccaatc ttacaggatt tatatgattt
     1621 gttgttaaaa caggaggaaa gcgttggtcg gaaattagcg actgaaatgg agatatatgt
     1681 aacaggttct ctaaatgtat ttaatcatcg ttcaaatgtt gacttgaata aatcacttct
     1741 atgcttcgat ataaaagagt tggggacaca attaaaaaag attggtatgt tagttattca
     1801 agaccaggta tggaacaagg tttcacaaaa taggcaaagt caaaaatcta cacggtatta
     1861 tattgatgaa tttcacttac tgctaaagga ggaacaaaca gctcaatatt cggttgaaat
     1921 ttggaagagg tttcgtaagt ggggagggat tccgacaggt ttaactcaaa acgtaaaaga
     1981 ccttttagcg agtaaggaaa tagaaaatgt ctttgataat acggacttta tcatgatgtt
     2041 gaaccaggct tcaggggaca gagaaattct agctagaaaa ctaaaaatat ctccctatca
     2101 gctcaattat gtaaccaact ctaatgctgg agagggtcta ctattctttg gaaataccat
     2161 tgttcccttc ttagaccaat tccctaagaa tacactcttg tatcaaaaga tgacaaccaa
     2221 accagaagag gtcaggaagg gctaggtgaa ttatgggaaa gtcaaaacga atggtccgag
     2281 agaccaataa gaagcattat acaaaatcaa aacctgacat tagtcaacgg aaggaagagc
     2341 aagatttttc tggaagaaca gttgatgaga gtgttaaatt tcaacaccag attattcata
     2401 aagaaagtaa attagagagc aggaaaaatc aagttgaatc ttctggaaag aggcggaaaa
     2461 atattaatat tgtggatgac aagtctacac aaactccttt acctattcat gataaaaatg
     2521 aatcaactat tcaaattaca gatctacaag atgaaatagt taataaaaat tatttacatc
     2581 agccctctcc accagaagca cccctagttg aatcaaagcc aataagtcat aagaaacaag
     2641 tccaattaca acaacggaaa gcctttgaaa atcagcaaaa tgttgaatca aggaacgata
     2701 ttgagttcac cgagcagaat ggccataatt ctgaaataca cgttagttct gatggggatc
     2761 ttgcacaaac tggtaatgaa caagaagttc aacaaataca atttgaaatt gtgcaccata
     2821 atccagtaaa ttccagcgaa ctaaatcact caaaggaatt gaatttcgga ccaacttcaa
     2881 gtcaaactca ggcacaaaat aattctcaac cgactgaatc tattgataga aaaattcatg
     2941 atactccgtt tcgtaagttt gatacaaatc aaagtagaat cattgatact gaaacaaagt
     3001 ttacccatcg gagaaatgag gggagagcaa aattaattca caatcagaat caaggaacgc
     3061 cgtcccagtt taagaataaa aaggggaaga aacatgttca tggcgattca gttcatgaaa
     3121 gtcaaaacat tctttcggaa ttacctattt ctcaatttga ttcttctaca gattcagttc
     3181 caataaaaac ttatccaaga gtgagtgatg agtctaattc tattattgat caagcagata
     3241 atgagagtgt ctttgatgtt tcacttctac aaagtcaatt gagaaaagat aataagaaat
     3301 ttcaaccgag taattctaaa ctttctaaaa aatcaggtgt caaaataata gaggcaaaaa
     3361 atagccaaag tcaacaaatt gaaaaaactg taactcttac agatgggggt aaacaaagtt
     3421 caaaccaatt tcagtcaaat cttaatgaaa attcgataat gattgaacag ataagttatc
     3481 ccaacatcga ctggtctaaa aaacttcatc tacctcaaat taaactattc tctagagata
     3541 ttttgcactc taatgctgta agcggtaaga ttcagaatga tgagagtaaa ccattgacca
     3601 gaaaaggtaa gagattatca aatagactga gcaataaagc aacaaaatcg aatattgctc
     3661 gtagtttata tcctgataca actagtgaca aaaatagaaa aactgtatct aaatcatcca
     3721 aaacgaaatt gagtatcact ccaattcaag ttgatggaag accaaaatat tattttgaaa
     3781 ctacaacaaa aaaacaattt tataaagggc gaccttcttc aaccaacaac attaaggcga
     3841 ataaatttag acgtcaaaga ttattaaaag atcagttaca aattcatgaa aagttgaatt
     3901 ccttagtaat tgagccaata gagtttgtaa aggataagaa gaatcgtcgt ttgcttaaaa
     3961 aaattgaaca agcaaaactt ggcagaataa aatcaagccc acttaaaaaa gtagtgtcag
     4021 gattggccag cagtgagttt ctagcagcaa gttatcttca agcaggagct gatgataatg
     4081 tggcagttga tgcaacttcg aaagtattga acatcaatgc tgctatacta atgagacttg
     4141 caaaaaaatc gaatcgtaat cagacgataa agaaattgac taagaaggct aaggttagac
     4201 aatcgcaatt ggaatttcgg cataagtatc gattgatgaa ggaagataag atttttcaac
     4261 ggcaatcatt ctatcgaaag tcaatgtatc gacagcggat gaagcaacaa attcgtaaca
     4321 agcacttccc tagaattaga gacaggataa aggaagaact aaaaaaactg ttaaatcaaa
     4381 tggtacagtt cattaccaca aagtggaaga gtattttggt attggctttg gctttattct
     4441 ctctattgac agttgtatat tcaaatagtc aatttatgtt tggagcttta tcatcagttt
     4501 cttatcagct taccactaca tcctatcttt cttcagagga aatgcttgtt gcattgaatc
     4561 acatattctc tacctatgaa ttgaatttat ccaatcaatt ggcaagattg aaggaatcga
     4621 aacctggata cgatgaatac atcgttaaag ggagggaaca aattggtcat gatccacatg
     4681 tcttattagc ctatttgaca gctagatttg gggaaataaa agaccaaaca agtgttgaac
     4741 cagcgatgaa acaactattt gttcagatat atcaggtttc ctatcgtgaa gagagggaaa
     4801 tccgttatcg aaaggtcaag gaagaagttc ttgacactag cggtaagaaa acgattgttg
     4861 agaaagaagt tccctatacc tatcgaaaat tgatagcaac agtttcaaaa aatgacatgg
     4921 atgtagttat tcagtcaata tttgccggca gtcctagcaa tttagaacat tataggattc
     4981 ttagggaaac aaaagggaat ttagaaactg cctttccttc gggaacgacg atcagtcttc
     5041 ctggaaatgt atcatcggta tatgatgtca atttaacagg cggaaatttt ccaccaccaa
     5101 accctaatca tgttgctgct ctaaatggcg gttaccctgg gcaatgtact tggtatgttt
     5161 ataatcgatt ctcacaactt ggaaagccaa tccaacattc accaatggga aatggtggcg
     5221 agtgggcgtt ctatgctgct aaatacggtt atccagtcag tagagaggca cgagctggta
     5281 cagcagtctg tatgcctcca actgtaccat atgcagatcc gacttacggt cacattgcgt
     5341 ttgtagagcg cgttaaccct gatggtagta ttgttatctc agaaatgaat gtaaaagggg
     5401 aatttgtgat ttcaacaggc tatctaccac gtgagatggc ggcacaatgt tattacatta
     5461 attttggatt ataaggagaa atatatgcgt aaagatattc aaatgttaca aaaaaagaaa
     5521 gaaaaattac ttgaaaaaaa gcaaaaaatt gaccaagatt tggctcaagt tttacaagaa
     5581 gaacaagatt tgatttatga agaagtcata gttgtttttg agaaatctaa tctatccctc
     5641 gaagaattta ctaaaaaagt tttatttgaa aaggagttgt cgcatgaaac gattatttag
     5701 ttttgtgaga gtagctagtt tggttatgtt atttagcggg tgccaaacta tttatgcaag
     5761 ttcacctagt tcagagccaa atagtcagcc agtagcaatt tctgtacatc cacaagcctc
     5821 agagcaaagc aacgccaagg gccaagtcac agaaaatgta gatgccaata atcaagatta
     5881 tgaaattact gattcagttg ctactaagcg gcagtttgtc acttttacaa gtaagtcagg
     5941 aaaggtgttt cacctgatta tagatcatga caaaggtgga caaaatgtac aattattgac
     6001 agaagtatct gaacaggatt tacttaacct tgttgaatca actgatactg ttgcagtcaa
     6061 accacaaaag gttgaagaag tcgttgatga taaacctgta aaaaatgaag aaccaaaaca
     6121 aaaatcttct gctggaagtt acattattat cggtctattt ctccttggtg ttttgtgtgt
     6181 aggatattat ataaaagtca ttaaaccaaa gaaagaacac cactttgaag aatttgaaga
     6241 agatgatgac tatataagtg atggtgaaga agaggtttga cattgttttc cttttttgta
     6301 taatgaaatt aga
//
LOCUS       DBNFEG010000125         6233 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.125, whole genome shotgun sequence.
ACCESSION   DBNFEG010000125 DBNFEG010000000
VERSION     DBNFEG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.125"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(41..1039)
                     /gene="mraY"
                     /locus_tag="AC1PNG_001611"
     CDS             complement(41..1039)
                     /gene="mraY"
                     /locus_tag="AC1PNG_001611"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIP6702103.1"
                     /translation="MQFALMSGLVAFLATVLLIPRFITFYQAKRIEGQQMHEDVKQHQ
                     FKAGTPTMGGTVFLVVAILVSLLFATAFQLLTGGVLAILFILALYGVVGFLDDFLKIF
                     RKINEGLNPKQKLALQILGGIVFYFVHVRGAGGGELNVFGHMVHLGVLYFPFVLFWLV
                     GFSNAVNLTDGIDGLASISVVISLLAYSVIAFNEQKFDILLVCVTMIGGLLGFFVYNR
                     KPAKIFMGDVGSLALGGMLATISIALRQEWTLLLIGLVYVIETSSVMLQVSYFKYTKK
                     RFGEGRRIFRMTPFHHHLELGGLTGKAEKWSEWKVDFFLWSVGLIMSLITLAILYL"
     gene            complement(1041..3290)
                     /gene="pbp2X"
                     /locus_tag="AC1PNG_001612"
     CDS             complement(1041..3290)
                     /gene="pbp2X"
                     /locus_tag="AC1PNG_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2X"
                     /protein_id="HIP6702104.1"
                     /translation="MPRRNNKLLRYVLKKRYVPSKNRRRVGQSLLLLTVFVFFLFLIN
                     FAYIIGTDSKFGVDLSERADAVHQREVTVQAKRGTIYDRTGIPIAEDSTTYTVYAIID
                     TEYVSELKKVLYVQSSQFSKVAEIFKKHLGMETAYVMEQLNQPELKQVYFGTLGKNIS
                     YSTMSSIQEEMKAAGIEGIAFNASPGRMYPNGNFASIFVGLANPVENKDGSHSLQGVS
                     GLEYYLNDILAGQDGKVIYEKDSQGRVLPGTEKVETETVNGQDVYTTISADLQRSLET
                     NMNTFFDNAKGRYANATLVSAKTGEILATTQRPSYDSDTKEGLGQEGLLERSLLYQEA
                     FEPGSTMKVLTVASAIDNGTFDPNAYYTNNEYVIADAKINDWTLNAGYSAVTLNYAQG
                     FSLSSNVGMTLLQQQMGDEKWLNYLTKFRFGYPTRFGMGDEAPGMVPEDNIVSIAMSS
                     FGQGISANQAQMLRSFTSIANNGIMLEPKFISALYDPNTDTARVSSKEEVGNPVSEQA
                     ADDTLRYMINVGTDPVYGTLYSHDLQGPAIQVDGYDIAVKSGTAEIASEDGQGYIKGA
                     YINSVVAMIPAEDPEFIMYVTIRQPEVLNVLSWRDIVSPTLKEAMLLKDNLNLDSPAP
                     ALARVANETEYQLPDLIGKAPGESAEELRRQLVQPVILGNGAEIKKVSVEKGSKVIAN
                     QQVLLLTNKFEEMPDLYAITKENMDIFAEWTGIEVTYKGTGSKVVDQSVEVGTALNKT
                     KKITITLGD"
     gene            complement(3290..3613)
                     /gene="ftsL"
                     /locus_tag="AC1PNG_001613"
     CDS             complement(3290..3613)
                     /gene="ftsL"
                     /locus_tag="AC1PNG_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HIP6702105.1"
                     /translation="MLQEKRREATMRVIGDKIRTFTRVEKAFYSSIVLSGLALAIGII
                     FMQTRLLQLQSGMAKVNQDISQKQIEINDAKQAVNELTRSARLMEIAEKAGLTFNNDN
                     IGVAE"
     gene            complement(3624..4574)
                     /gene="rsmH"
                     /locus_tag="AC1PNG_001614"
     CDS             complement(3624..4574)
                     /gene="rsmH"
                     /locus_tag="AC1PNG_001614"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIP6702106.1"
                     /translation="MTKEFNHTTVLLHETVDMLDIKPNGIYVDATLGGAGHSEYLLSQ
                     LTDGGHLYAFDQDQTAIDHAHIRLASYIEKGQVTFIRDNFRNLKTRLAELGVTEIDGI
                     CYDLGVSSPQLDERERGFSYKQDAPLDMRMNREGHLTAYDVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQARAVKPIETTTELAELIKSAKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAIDLLALDGRISVITFHSLEDRLTKQLFKEASTIDVPKGLPFIPD
                     DLKAPLELVNRKPILPSQEELEANNRAHSAKLRVAKKVHK"
     gene            4845..5033
                     /locus_tag="AC1PNG_001615"
     CDS             4845..5033
                     /locus_tag="AC1PNG_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIP6702107.1"
                     /translation="MNRVKDYRLLLGISQLDLAKAIGVSRQTVNMIENNKYNPSLDLC
                     INLAKALQTDLNSLFWNE"
     gene            5049..5249
                     /locus_tag="AC1PNG_001616"
     CDS             5049..5249
                     /locus_tag="AC1PNG_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702108.1"
                     /translation="MKKNIRQALISTLIFIPLFVLFQLWGNSGAEILHTISQAKFWLQ
                     LLIAGTIYFLGFVYILPLFDQK"
     gene            5260..6048
                     /locus_tag="AC1PNG_001617"
                     /pseudo
     CDS             5260..6048
                     /locus_tag="AC1PNG_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
     gene            complement(6053..>6233)
                     /locus_tag="AC1PNG_001618"
     CDS             complement(6053..>6233)
                     /locus_tag="AC1PNG_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIP6702109.1"
                     /translation="SKLKDLCEEQGHRLLPLPPYSPEYNPIEKIWAHIKKHIRRVLTN
                     CDTFLEVLSSCSCFS"
BASE COUNT         1806 a         1439 c         1186 g         1802 t
ORIGIN      
        1 gtcaaggtaa aaaaacagca tttctgctgt ttctgacatt ttatagataa agaatagcta
       61 gggtaatcaa gctcatgatg agaccgactg accagaggaa aaagtcaact ttccactcgc
      121 tccatttttc agcttttccg gtcaaacctc caagctccaa atggtgatga aaaggagtca
      181 tacggaagat acgacgacct tctccaaagc gttttttggt gtatttaaaa tatgaaacct
      241 gcaacataac agaggacgtt tcaatgacat agaccaggcc gataaggagg agagtccatt
      301 cctgacggag ggcaattgaa atagttgcca acataccgcc gagagccaag cttcccacat
      361 cccccatgaa aatcttcgct ggtttacggt tataaacaaa gaaaccgagc aagccaccaa
      421 tcatggtcac acaaactaga agaatatcaa atttttgctc attgaaggcg atgacagagt
      481 aagctagaag gctgataaca actgagatag aagccaatcc atcaatgcca tctgtcaaat
      541 taactgcatt tgaaaaaccg accagccaga acagaacaaa tgggaaatag agaactccca
      601 aatgtaccat gtgaccaaat acattcaatt ctccgccacc tgctcctctt acgtggacaa
      661 agtagaagac aatacctcct aaaatttgta gagcaagctt ttgtttagga ttaagacctt
      721 catttatttt acggaaaatt tttaaaaagt catccaaaaa tcctactaca ccataaaggg
      781 caaggatgaa gaggatggca aggacaccac ccgtcaacag ttgaaaggcc gttgcaaaca
      841 acaagctgac aagaatcgca acaacgagaa aaactgttcc ccccattgtt ggtgtacctg
      901 ccttaaactg atgttgtttc acatcttcgt gcatctgttg cccctcaatg cgtttggctt
      961 gataaaatgt aataaatcgt gggattaaga gaacggttgc tagaaaagct accaatcccg
     1021 acataagtgc gaattgcatg ttagtctcct aaggttattg ttattttctt ggttttattc
     1081 aaagcggtac cgacctctac actttggtcg actacttttg aacccgttcc tttataggtc
     1141 acttcgatac cagtccattc agcaaatata tccatattct ctttggtgat tgcgtacaag
     1201 tctggcattt cttcgaattt atttgtcagg agtagaacct gttggttggc gattacctta
     1261 cttccttttt cgactgaaac ttttttaatt tcagcaccgt ttccaaggat aactggttga
     1321 actaattgac ggcgaagttc ctcagccgac tcgcctggtg ccttaccaat gaggtctggc
     1381 aattgatact ctgtctcatt agcgacccgt gccaatgctg gtgcaggtga atccagattc
     1441 aaattgtctt tcaacagcat cgcttctttc aatgtaggac tgacaatgtc acgccaagac
     1501 aggacattta atacttccgg ttggcgaatg gtcacataca tgataaattc tgggtcctca
     1561 gcagggatca tggctactac agagttgata taggcgcctt taatgtatcc ttgaccatct
     1621 tcgctggcaa tttccgccgt acctgatttg actgcaatat catagccatc aacctgaatt
     1681 gcaggcccct gtaagtcatg tgagtagagt gttccataaa cagggtcagt accgacatta
     1741 atcatatagc gaagtgtatc atcggcagct tgttcagaaa ctggatttcc cacttcttct
     1801 ttcgaagaaa ctctagctgt atctgtatta ggatcataaa gagctgagat aaatttaggc
     1861 tctagcataa taccattgtt cgcaatggat gtaaaactac gaagcatctg tgcttggttt
     1921 gcagaaatcc cctgaccaaa cgatgacatg gcgatagaaa caatgttatc ctctggtacc
     1981 attcctggcg cctcgtcccc cataccaaaa cgcgttgggt agccaaaacg gaacttagtg
     2041 aggtaattta gccatttttc atctcccatc tgttgctgca ataaggtcat cccaacgtta
     2101 ctggataggg agaagccttg cgcataattg agagtcacag ctgaataacc tgcattgagg
     2161 gtccagtcat tgattttcgc atctgcaata acgtactcat tattggtata atatgcattt
     2221 gggtcaaagg ttccgttatc gatagctgac gctactgtta gcaccttcat tgttgaacca
     2281 ggctcaaagg cctcttgata taaaagtgac cgctctagca ggccttcttg tcccagacct
     2341 tctttggtat ctgaatcata gcttggcctt tgggtcgtcg ccagtatttc acccgtcttg
     2401 gctgaaacca aagttgcatt cgcatatcgc cctttggcgt tatcaaagaa ggtattcata
     2461 ttggtttcaa gagaacgttg cagatcggct gaaattgtcg tatagacatc ctgcccgttt
     2521 actgtctccg tttcaacctt ttctgtccca ggtaggacac ggccttgact atctttctcg
     2581 tagataacct ttccatcttg accagctaga atatcattca gataatattc caacccactc
     2641 acaccctgta aactatgact accatccttg ttttcaaccg gattagctaa cccgacaaaa
     2701 attgaagcaa aatttccatt cggatacatc cgtcctggac tagcattaaa ggcaatccct
     2761 tcaatacctg ccgccttcat ctcttcttgg attgaggaca tcgtgctata ggagatattc
     2821 ttacctaagg tcccaaagta tacctgcttc agttccggtt gattcagctg ctccataaca
     2881 taagctgtct ccattccgag atgcttttta aagatttctg ctactttgct gaactgagaa
     2941 gactgcacat acaaaacttt tttcagttct gacacatatt ctgtgtcaat gatggcatag
     3001 actgtatagg tcgttgaatc ttctgcaata ggaattcctg tacggtcgta tatcgtcccc
     3061 cgtttagctt gaacggtaac ttcacgctgg tgtaccgcat cagctctctc tgacaagtcc
     3121 accccaaatt tactatcagt gccgataata taggcaaagt tgattaaaaa gagaaaaaag
     3181 acgaaaaccg tcaaaagaag taggctctgt cctactcttc gacgattttt tgacggaaca
     3241 tatcgttttt tcaagacata ccgcaagagt ttattatttc ttctgggcat tattcggcta
     3301 ctccaatatt gtcattatta aaagttaatc cagctttttc agcaatttcc atcaagcgag
     3361 ctgaacgtgt caattcattg acagcttgct ttgcatcgtt aatttcaatc tgtttttggc
     3421 taatatcttg attgactttg gccatgccag attgaagttg caaaagccgc gtttgcataa
     3481 agataattcc tatcgcaagg gctaatccag ataatacgat acttgaatag aaggcttttt
     3541 ctacacgtgt gaaggttcta attttatcac cgataacccg catagtggct tctctacgtt
     3601 tttcttgcaa catagactct ctcctattta tgtactttct tagctacacg cagtttagct
     3661 gaatgcgcgc gattatttgc ctctaattct tcttgactag gtaagatagg cttacgattg
     3721 accaattcaa gtggcgcctt caagtcatct ggaataaatg gcaaaccttt tggaacatca
     3781 atcgtacttg cttctttgaa gagttgtttg gtcaaacgat cttctaaaga atggaaagta
     3841 atcacagaaa tccgtccatc caaagccaat aagtcaatgg cctgctggat agattcgtct
     3901 gctgccccca actcatcgtt gacttcaatc cgaatcgctt ggaaaatctg cttggcagga
     3961 tggcccttct tcttaagttc cttggctggc ttggcagatt tgatcagctc tgccaactca
     4021 gtcgttgttt caatcggctt gaccgcacgc gcctgttcaa tcttacgagc aatctgcttg
     4081 gaaaatttat cctcaccgta tttaaagaaa atccgtacca agtcgtgata atcatagtta
     4141 ttgaccacat cataagccgt caaatgtccc tcacgattca tccgcatatc aagcggagca
     4201 tcttgtttgt aagaaaagcc acgctcccgc tcatccaact gtggactaga tactcctaaa
     4261 tcataacaaa tcccatcaat ctctgttaca cctagctccg ccaagcgagt cttgagatta
     4321 cggaaattat ccctgataaa ggtcacttgt cccttttcaa tgtaggatgc caagcgaata
     4381 tgggcatgat caatagcagt ctgatcctga tcaaaggcat aaagatgccc accatccgtc
     4441 aactgactga gcaggtactc actatgaccc gctccaccca gcgttgcatc tacataaatt
     4501 ccattgggtt tgatgtctaa catatcgact gtctcatgca agagaacagt tgtgtgatta
     4561 aattccttgg tcatatcttt tctattgtac cacaaattaa aataaaaagt tgttgcaaac
     4621 tttttacaaa aaaactgtca aatatagttg acaacttata tgaacaggta tatactaaca
     4681 gtaaagaaac atgtcacata tagttgacat caacagggag gtctcccaca tttttttaca
     4741 gttatatgtc agatataatt gacaatattt taaaaggata gtcaacctat cctctcattt
     4801 ttagccaagc atgtcagata taattgacat agaaaggagg gcacatgaac cgtgtcaaag
     4861 attatcgcct actgctaggc atttcccagc tggatctagc caaagccatc ggcgtatcca
     4921 ggcagaccgt caacatgatt gaaaacaaca aatacaaccc ctcgctagac ctctgtatca
     4981 acctagccaa agctttacag actgacctca acagcctctt ttggaatgaa tagaaaggaa
     5041 gaaaaaacat gaaaaagaat atacgtcaag ctcttatctc tacccttatt tttataccgc
     5101 tctttgtcct ctttcagctt tggggcaata gcggagcaga aatcttgcac accatcagcc
     5161 aagcaaaatt ttggctacaa ttgctgattg ctgggaccat ctacttctta ggctttgttt
     5221 acatcttacc attgttcgat caaaaataag gagctgctta tgaaaaaaga aacctttcaa
     5281 gataaattaa tcaaacgatt ttacggtatc gcagggccct tggatgaatt tcgccaaaaa
     5341 gaagccttcc gactaggcaa tacctgcttt atcctgctct tttggggcac catggctata
     5401 accctgctgg cacttgccct atccaaacgc tacccagaag tcgttgccta tggctaccca
     5461 acggctctac tcctatccac tctttccgcc agtatgtata tgacatccaa gatgcgccac
     5521 agtcaagtgg acagtttaga tgtagaagaa ctgactacca aggaacaaaa gaaattcaag
     5581 ggagcaagta tcaaattcgc cctctatttc acctgtggta tgtatatctg gaacacggga
     5641 tttgatgcct ggatggaagg actcaatcca ctagaccatt tatttgacct ccgaaaattc
     5701 cttgcagcct gtctagtcgg tgttttcatg ggaatttata tcgaaatcac tcttcgtaaa
     5761 cgcatgaaaa aggcagaaaa actaactgtc agctctgcta ttgccaaaga agaacccaag
     5821 tggatcaaaa atatgattaa acgtttttat ggtatccgcg gtcctttaga cgaataccga
     5881 cgggccgagg ctgatgctat cggcggacaa gcctttatct actacttcta cttcctagct
     5941 cttggaaatg ccatcgccta cttcctagcc taccgctacc ctctggaggt ggctgcctac
     6001 tatccaatga tcattgcctt cttcagtatt atcctaattg gtatcgttat agtcaactga
     6061 aacaagagca ggacgaaagt acctcaagaa aagtatcgca atttgtcaat actcttctga
     6121 tgtgtttttt gatgtgagcc catattttct caatgggatt atattccggt gagtaaggag
     6181 gaagtggtaa aagtctatgt ccctgctcct cgcataaatc ttttagctta ctc
//
LOCUS       DBNFEG010000126         6122 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.126, whole genome shotgun sequence.
ACCESSION   DBNFEG010000126 DBNFEG010000000
VERSION     DBNFEG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.126"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            310..591
                     /locus_tag="AC1PNG_001619"
     CDS             310..591
                     /locus_tag="AC1PNG_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIB"
                     /protein_id="HIP6702110.1"
                     /translation="MKKVAAVIGVKITKKTVEKTIANAVPILGGLISGGLTYFTFQPM
                     GGMLADTFVKKINGDFDGELELTEAFKASLEEEKELGIIEAEFTEASES"
     gene            671..2014
                     /locus_tag="AC1PNG_001620"
     CDS             671..2014
                     /locus_tag="AC1PNG_001620"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIP6702111.1"
                     /translation="MKFTDMKLKPYIQEALKEINFVQPTEVQEKLIPVVLSGRDLIGE
                     SKTGSGKTHTFLIPIFQKLSEELDQVQAVITAPSRELATQIYQAARQLASHSDIEVRV
                     TNYVGGTDKNRQIDKLQASQPHIVVGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEQIKTHTVISDTIE
                     NWLISTKGRDRNAQILEVTKLMQPYLAMIFVNTKTRADELHSYLTANGLRVAKIHGDI
                     PPRERKRMMNQVKNLDYQYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLSGIAITLYQPSDDADIRELEKLNIKFLPKEMKNGEFVDTYDRDRRANREKSREK
                     LDLEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRVEARAKGRAERKAKRQTF"
     gene            2032..2256
                     /locus_tag="AC1PNG_001621"
     CDS             2032..2256
                     /locus_tag="AC1PNG_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HIP6702112.1"
                     /translation="MLQNILSFYLQGLLIASLLIITSSLVWFIWRATKGVDKTLQERQ
                     DFLFDLLMINVMTIPIAAFGVVGILLMFKA"
     gene            2270..3187
                     /gene="trxB"
                     /locus_tag="AC1PNG_001622"
     CDS             2270..3187
                     /gene="trxB"
                     /locus_tag="AC1PNG_001622"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIP6702113.1"
                     /translation="MYDTVVIGAGPAGMTAALYAGRSNLKVALLERGIYGGQMNNTAE
                     IENYPGYDHISGPALAEKMFEPLEKFGVDHIFGTLVRIEEEGQIKKVITEDGVLETKT
                     VVLAMGAKHRLLGIPGEDTYNSRGVSYCAVCDGAFFRGQKLLVVGGGDSAVEEALFLT
                     QFAESVTIVHRRDQLRAQKVIQDRAFANEKINFIWDSVVEEIKGDDLRVQSVVIKNVK
                     TEEVSELDFGGVFIYVGLDPMTDTVADLGITDEAGWVITNEKMETTQSGIYAIGDIRQ
                     NQLRQIATAVGNGAVAGQEVYNYITELAE"
     gene            3197..3736
                     /locus_tag="AC1PNG_001623"
     CDS             3197..3736
                     /locus_tag="AC1PNG_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIP6702114.1"
                     /translation="MFHIYDIQKNLDGISFDKTLDLQEELQARNGEVLGLSPVEVTGN
                     IRFESGFFFLDYQMTYDITLASSRSLQPVVLHEVQNVNELFVANEAVLKEQDLIDEDM
                     VLVVEDDHIVLDESVADNILLAIPIKVLTPEEEAGQELPSGQAWALMTEEDFQQKAQE
                     KKEANSPFAQLQGLFGDED"
     gene            3833..5260
                     /gene="gndA"
                     /locus_tag="AC1PNG_001624"
     CDS             3833..5260
                     /gene="gndA"
                     /locus_tag="AC1PNG_001624"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HIP6702115.1"
                     /translation="MTKANFGVVGMAVMGRNLALNVESRGYSVAIYNRSADKTEDVVA
                     SNPGKNLVPSYDVESFVASIEKPRRIMLMVQAGPGTDATIQALLPHLDEGDILIDGGN
                     TFYEDTIRRSKELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGAPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSVDEMAD
                     IFTEWNKGELDSYLIEITADILKRKDDQGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKDERVAASKVLPKPAPFAYEGDKAELVEKIRQALYFSKI
                     MSYAQGFAQLRVASKENNWNLPFGEIAKIWRAGCIIRARFLQKITDAYGRDEDLANLL
                     LDEYFLDVTAKYQQAVRDVVALAVQAGVPVPTFSAAITYFDSYRAENLPANLIQAQRD
                     YFGAHTYNRKDKEGIFHYDWYSESK"
     gene            5342..6031
                     /locus_tag="AC1PNG_001625"
     CDS             5342..6031
                     /locus_tag="AC1PNG_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIP6702116.1"
                     /translation="MAKKILIAGKERNLSHFVSMELQKKDYLVDYASTGKEAMSLAHE
                     TDFDLILMSFQLSDMSSKELAAELLAIKPATVMIVVVEPTEVSQYGEEVLSYAVSYVV
                     KPFVISDLVEQISAIFRGRDFIDNNCKQVHMHAAYRDLKVDFQNRTVTRGDELINLTR
                     REYDLLATLMNSPEPVSREQLLERVWKYEAASETNVVDVYIRYLRGKLDLPHQDSYIK
                     TVRGVGYAMRD"
BASE COUNT         1838 a         1151 c         1419 g         1714 t
ORIGIN      
        1 ctcacatcaa tcccgaaaac tgcacgcatg atattacctc tttgtcttga atgattcctt
       61 gttttagtga tgtcattttc aatactcgac gtctggcgtc ccacatactt tgataacatt
      121 ctttctaaaa caggtgtctt gccagttttt gttgcgacgt ctagcgtcaa aaagccctac
      181 gacttaacaa gacacctcta ctttatcata aagaaaaagt agtgagtact ttctcccgtc
      241 ggagatttcc tcactactaa tcttagtatg tttttgacca gtcgagttct ttcccgctcc
      301 catcccttgg tgaaaaaggt agccgcagtg attggtgtga aaattaccaa aaagactgtt
      361 gaaaagacca ttgccaatgc agttcctatt ttgggtggtc tgatttcagg aggtttgacc
      421 tattttacct tccagcccat gggagggatg ttggcagata catttgtgaa aaaaataaat
      481 ggtgattttg atggcgaatt agaactcact gaggccttta aagcaagttt ggaagaagag
      541 aaggaacttg gaattattga ggcagaattt actgaagcaa gcgagagttg attaggacgg
      601 gtatccgtcc tttttgcttg tccacctaaa tcgtgctata atagagggaa atacaactaa
      661 agaggaaact atgaaattta cagatatgaa actcaagcct tatattcagg aggctttgaa
      721 agaaattaac tttgtccagc cgacagaagt tcaggagaaa ttgattcctg ttgttttgtc
      781 agggcgagac ttgatagggg aatcaaaaac aggttcgggt aagacccata ccttcttgat
      841 ccctattttc caaaaattaa gtgaggaatt ggaccaagtt caggctgtta ttacagcccc
      901 atctcgtgaa ttagctactc agatttatca agcggcacgc cagttggcaa gtcattcgga
      961 catcgaagtt cgtgtgacca attatgttgg cggtacggat aagaaccgtc aaattgacaa
     1021 gcttcaagct agtcagcctc acattgttgt tgggacacca gggcgtatct atgatttggt
     1081 caaatcaggt gaccttgcta tccataaagc caaaaccttt gtggttgatg aggcagatat
     1141 gaccttggac atgggctttt tggaaacagt tgataagatt gctggcagcc tgccaaaaga
     1201 cttgcaattt atggtctttt cagcgaccat tccgcagaaa ctccaaccgt ttttgaaaaa
     1261 atacctgtcc aatccagtca tggaacaaat caagacccat acggtcattt cagataccat
     1321 tgaaaactgg ctgatttcaa ccaaggggcg tgaccgtaat gctcaaatct tggaagtaac
     1381 caagctcatg caaccttact tggccatgat ttttgtcaat acaaagacac gtgcagatga
     1441 attgcatagc tatttgactg cgaacggtct tcgtgtcgca aagattcatg gggatattcc
     1501 tccacgtgag cgcaagcgga tgatgaacca ggtgaagaac ttggactatc agtacattgt
     1561 tgcaacggat ttagcagcac gtgggattga tattgagggc gtcagtcatg tcatcaatga
     1621 tgccattcca caggatcttt ccttctttgt tcaccgtgtg gggcgcacag gtcgtaatgg
     1681 tttatcaggc attgccatta ccctttatca gccaagcgat gatgcagata ttcgagagct
     1741 agaaaaactc aatatcaaat tcttgccaaa agaaatgaaa aatggcgaat ttgtcgatac
     1801 ctacgaccgt gaccgccgtg ctaaccgtga aaaatctcgt gaaaaactag atttggaaat
     1861 gattggcttg gtcaagaaga aaaagaaaaa agtcaaacca ggctacaaga agaaaattca
     1921 atgggctgtt gatgaaaaac gtcgcaagac caagcgagtt gaagcgcgtg caaagggtcg
     1981 tgcagaacgt aaagccaagc gtcaaacctt ttaaagtaat cgaggaaaaa gatgttacaa
     2041 aacattctaa gtttttattt acaaggattg ctaatagctt ccttgttgat tatcacttct
     2101 agtctggtct ggtttatctg gcgtgcgaca aagggtgtgg ataaaacctt gcaagaacga
     2161 caagatttct tgtttgattt gttgatgatt aatgtgatga caattcccat tgcagccttt
     2221 ggagtagtgg gaatcttatt aatgtttaaa gcgtagaagg aaaaagtata tgtacgatac
     2281 agttgtaatt ggtgcgggtc ctgcggggat gactgcagct ctttatgcag ggcgtagcaa
     2341 tttaaaggta gctcttttgg aacgtggtat ttatggtggt cagatgaaca ataccgcgga
     2401 aattgaaaac tatccaggct atgaccatat ttcaggtcca gccttggctg aaaaaatgtt
     2461 tgaacccttg gaaaaattcg gtgtggacca tatttttggc accctagtcc gtattgagga
     2521 agaaggacaa attaagaaag tgattactga ggatggtgtt ttggagacaa agacagtcgt
     2581 tctagccatg ggggcaaaac accgtttgtt aggtattcca ggtgaggata cttacaatag
     2641 ccgtggtgtc tcatattgtg cagtctgtga cggtgccttc ttccgtggac aaaaactctt
     2701 ggttgtcggt ggtggagatt ctgcggtcga agaagctctc ttcttgacac aatttgcgga
     2761 gtctgtgaca attgttcacc gccgtgatca acttcgtgct caaaaagtca tccaagatcg
     2821 cgcctttgcc aatgaaaaaa taaactttat ctgggatagc gtagtcgaag aaatcaaggg
     2881 cgatgactta cgtgtacaaa gcgtggttat caaaaatgtg aaaactgaag aagtcagtga
     2941 acttgatttc ggtggtgtct ttatctatgt cggtctggat ccgatgactg atacagttgc
     3001 ggacctagga attacagacg aagctggttg ggtcatcact aatgaaaaga tggagacgac
     3061 tcaatcaggc atctatgcca tcggtgatat tcgtcaaaat caacttcgtc agattgcaac
     3121 ggcagtagga aatggtgcag ttgctggaca agaagtctat aactacatca cagaactagc
     3181 tgaatagggg aatacaatgt ttcatattta cgatattcag aaaaatctag atggcatctc
     3241 ctttgataaa accttggatt tacaggaaga attgcaagct cgaaatggtg aagttctagg
     3301 cttgtcgcca gtcgaagtca ctggaaatat ccgttttgaa tcaggtttct ttttcttgga
     3361 ctatcagatg acttatgata ttaccttggc atccagtcgt tctctgcaac cagttgtttt
     3421 gcatgaggtc caaaacgtca atgaactctt tgttgccaat gaagcagttc ttaaagaaca
     3481 agatttgatt gatgaggata tggtactggt agtagaagat gaccacattg tccttgatga
     3541 gagtgtagca gataatattt tactggctat accaatcaaa gtcttgacac ctgaagaaga
     3601 agctggacag gaactaccat ctggtcaagc ttgggcttta atgactgaag aagattttca
     3661 acaaaaagct caagagaaaa aagaggccaa tagtcctttt gcccaattac aagggttatt
     3721 tggagatgag gactaactca gattggcaag atagttaaat tatctgaaaa ttcttgtcaa
     3781 ttttcagctt tcggtgtata ataaaagaga tacaaattag aggagtaaac aaatgactaa
     3841 agcaaatttt ggtgttgtag ggatggccgt tatgggccgc aaccttgcac ttaacgttga
     3901 atcacgtggc tactcagtag ctatttacaa ccgctctgct gataaaacag aagacgttgt
     3961 tgcaagcaac ccaggtaaaa acttggtacc aagttacgat gtggaaagct ttgtggcatc
     4021 tatcgaaaaa ccacgtcgca tcatgcttat ggttcaagct ggtcctggta cagatgcaac
     4081 cattcaagcg ctcttgccac acttggatga aggtgacatc ttgatcgatg gtggtaatac
     4141 tttctacgaa gacactatcc gtcgttcaaa agaattggca aactcaggta ttaacttcat
     4201 cggtacaggt gtatctggtg gtgaaaaagg agcccttgaa ggtccatcta tcatgcctgg
     4261 tggtcaaaaa gaagcttacg aattggtggc agatgtcttg gaagaaattt cagcaaaagc
     4321 accagaagat ggagcaccat gtgtgactta tatcggtcca gatggagctg gtcactacgt
     4381 aaaaatggta cacaacggta tcgagtatgg cgacatgcaa ttgattgcgg agtcttatga
     4441 cctcatgcaa cacttgctcg gtttgtcagt ggatgaaatg gctgacatct ttactgagtg
     4501 gaacaaaggc gaattggata gctacttgat tgaaatcaca gcagatattt tgaaacgtaa
     4561 agacgatcaa ggtcaagatg gaccaatcgt tgactacatt ttggatgcgg ctggtaacaa
     4621 gggaactggt aaatggacaa gccaatcatc acttgacttg ggtgtgccat tgtcattgat
     4681 tacagaatct gtatttgctc gttatatctc aacctacaaa gatgagcgtg tagcagcaag
     4741 caaggtcctt ccaaaaccag ctccgtttgc atacgaaggc gacaaggctg aattggtaga
     4801 aaaaatccgt caagcattgt acttctcaaa aatcatgtct tatgcacaag gttttgcaca
     4861 gttgcgtgta gcatcaaaag aaaacaactg gaacttgcca tttggtgaaa tcgcaaaaat
     4921 ctggcgtgca ggttgtatca tccgcgctcg cttcttgcaa aaaatcacag atgcctacgg
     4981 acgtgatgaa gacttggcaa acttgctctt ggatgagtac ttccttgatg taacagctaa
     5041 gtatcaacag gcagttcgtg atgttgttgc cttggcagtt caagcaggag ttcctgtacc
     5101 aacattctca gcagctatta cctacttcga tagctaccgt gctgaaaact tgccagcgaa
     5161 cttgattcaa gcacaacgtg actactttgg tgctcacact tataaccgta aagataaaga
     5221 aggaatcttc cattacgact ggtatagcga atcaaaatag aaaagatggg cgtggaagcg
     5281 cccttctttc tagtcataat acgattccta caccatttga aagaatagaa aaggatggac
     5341 aatggctaag aaaattttga ttgctggtaa agaacgcaat ctctcgcatt ttgtttccat
     5401 ggaattgcag aaaaaagact atcttgttga ttatgcatcg acagggaaag aggctatgtc
     5461 cttggcacat gaaacggatt ttgacttgat tctgatgagt tttcagcttt cagacatgtc
     5521 cagcaaggaa ttggctgcag aactattagc gataaaacca gccacggtca tgattgtggt
     5581 agttgagccg acagaagtta gtcaatatgg ggaagaagtc ctctcttatg ctgtttccta
     5641 cgtagtgaag ccctttgtca tcagtgattt agtggagcaa atctctgcga tttttcgtgg
     5701 tcgtgatttt attgacaata actgtaaaca ggttcacatg catgcggcct atcgtgattt
     5761 gaaggttgat tttcaaaatc gaacagtgac tcgaggtgat gaattgatta atttgacccg
     5821 ccgagagtat gatctgttgg cgaccttaat gaatagccca gagcctgtta gtcgagaaca
     5881 acttcttgag cgtgtttgga aatatgaagc ggcttctgag accaatgtcg tcgatgtata
     5941 tattcgctat ctacgtggta aactggattt gccacatcaa gattcatata taaaaacagt
     6001 tcgtggtgtc ggatatgcca tgcgcgattg atttagagta gagaggaggg aagctttccc
     6061 tctttctttt tctataagcg tgtgataaaa taatagccaa taagtaagaa tggaaaagtt
     6121 at
//
LOCUS       DBNFEG010000127         6114 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.127, whole genome shotgun sequence.
ACCESSION   DBNFEG010000127 DBNFEG010000000
VERSION     DBNFEG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.127"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(6..848)
                     /locus_tag="AC1PNG_001626"
     CDS             complement(6..848)
                     /locus_tag="AC1PNG_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HIP6702117.1"
                     /translation="MFTISNIRERARQTLNETPGIYQVAVIPVTISVIVQLISFSRNS
                     LAGVSAEELASPSYFISTSLFPVFYGLLLGMLSLSVIWTIFNIIKGGKTTTSFKDSLS
                     IFNHKDFWKIFRTYILKSFLLFLWGLLFYLGIGLLIGAGTMITIIAASSQSLDPDALP
                     MEILAILGLMAMGGILLMVLGTALYIPQIYAYSQVEYILFEQLEREEYTDAFSIIRSS
                     RKLMKGYKFKRFTLDLSFIGWFLLVIITFGLAGLYVWPYHYAAQMHFHEEILDDQAKK
                     MSYV"
     gene            1302..2444
                     /gene="tgt"
                     /locus_tag="AC1PNG_001627"
     CDS             1302..2444
                     /gene="tgt"
                     /locus_tag="AC1PNG_001627"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIP6702118.1"
                     /translation="MTDVPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTMSPEELKAMGSGIILSNTYHLWLRPGDELVARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNISEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFPGYSI
                     GGLAVGETHDEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSRGRLVVKNAQFAEDFTPLDPECDCYTCKNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLINLMKNVRQAIMDDNLLEFRQDFMEKYGYGKNGRNF"
     gene            complement(2777..3862)
                     /locus_tag="AC1PNG_001628"
     CDS             complement(2777..3862)
                     /locus_tag="AC1PNG_001628"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIP6702119.1"
                     /translation="MSKLHHVKSYLEANKMDLAIFSDPVSIYYLTGYHSDPHERHMML
                     FVMPDHDSLLFLPALDVERAVATVDFPVAGYMDSENPWQIIKSKLPQKSFSVICAEFD
                     NLNLTRYHGLQSIFSQPFSDITPLINTMKLIKSRDEIEKMLVAGEFADKAMQVGFNNI
                     SLNVTETDIIAQIEFEMKKQGISKMSFDTMVLTGDNAANPHGIPSTNKIENNALLLFD
                     LGVEALGYTSDMTRTVAVGKPDQFKKDIYNLTLEAHMAAVNMIKPGVTAGEIDYAARS
                     VIEKAGYGEYFNHRLGHGLGMSVHEFPSIMEGNDLVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGYVTKNGFEVFTKTPKELLYFEG"
     gene            4073..5077
                     /gene="ccpA"
                     /locus_tag="AC1PNG_001629"
     CDS             4073..5077
                     /gene="ccpA"
                     /locus_tag="AC1PNG_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HIP6702120.1"
                     /translation="MLNTDDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLE
                     VIDRLDYRPNAVARGLASKKTTTVGVVIPNIANAYFATLAKGIDDIADMYKYNIVLAN
                     SDENDEKEINVVNTLFSKQVDGIIFMGYHLTDKIRAEFSRSRTPIVLAGTVDLEHQLP
                     SVNIDYAAASVDAVNLLAKNNKKIAFVSGPLVDDINGKVRFAGYKQGLKDNGIEFNEG
                     LVFESKYKYEEGYALAERILNAGATAAYVAEDEIAAGLLNGVSDMGVKVPEDFEIITS
                     DDSLVTKFTRPNLTSINQPLYDIGAIAMRMLTKIMHKEELENREVVLNHGIKVRKSTK
                     "
     gene            complement(5509..>6114)
                     /locus_tag="AC1PNG_001630"
     CDS             complement(5509..>6114)
                     /locus_tag="AC1PNG_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIP6702121.1"
                     /translation="DNIETSEKQMPSVVNENAVTPEKQMTNKENDKVVISEKQMPSIV
                     NDMVVTPQEQMANKENDEVVISEKQMPSIVNDMVVTPQEQMANKENDNVETSEKQMPV
                     NEKDNAVTPERQIANKEKENIETSKKQIPVNGNNQNGTVGENSNTKPTTEKTDKQETS
                     TFKTETAKQILPVTGEKGSLWLLTSGIIGLAIALFTRKRKL"
BASE COUNT         1849 a         1161 c         1274 g         1830 t
ORIGIN      
        1 ttattttata cgtaactcat tttcttagcc tgatcatcta aaatttcttc atggaagtgc
       61 atttgtgcag catagtgata cggccataca tataaacctg ccaatccaaa tgtaataatg
      121 acaagaagga accaaccaat gaaagataaa tcaagtgtaa agcgtttgaa tttgtaacct
      181 ttcatcaact ttcggctaga ccgaatgatt gaaaaggcgt ctgtatattc ctcgcgttcc
      241 aattgttcaa acaagatata ttctacctgt gagtaagcat agatttgtgg aatataaaga
      301 gcagttccta aaaccataag taggatacct cccatggcca ttagccctag tatggcaagg
      361 atttccatag gaagtgcatc agggtctaag gattgactgg atgcagctat aatcgtaatc
      421 atcgttccag caccgataag tagaccgata ccaaggtaaa acagtagtcc ccaaaggaag
      481 agcaagaaac tttttaaaat gtaagttcga aagattttcc agaaatcttt atgattgaaa
      541 atgctcaagg aatctttgaa tgatgtggta gttttgcccc ctttgatgat attgaagata
      601 gtccatatga ctgataatga caacattcct agtagcaatc cgtaaaatac tggaaacaaa
      661 gaggtagaaa taaagtagct cggactagca agttcctcag ccgacactcc agcaaggcta
      721 ttgcgagaga aagaaattaa ctgaacgata acagaaatag tgacggggat gacggccact
      781 tgatagatac caggtgtctc gtttagcgtt tggcgagccc gctcacgtat attagaaatt
      841 gtaaacatct acataacctc cttatcttaa cattgtaaca taattctaga tgacaagcta
      901 ggcttttttt gatagactgg aaggtgctgt ggtatagtcg tttagtttgc ttttatgaag
      961 tacggcagag cgagttcaaa tactctagtg gagtgtttga agttggaaat aaggaaacga
     1021 agtttcctcg ctcgtcgaag caatcaaaga taaactaaat gactataatt tgtcagtttg
     1081 ttgtgatgcg cctatctatt tactattaaa taagtagaag acaaggtaaa tatcgctgaa
     1141 ataagcgaga acattaaaga cattctgatg gattagacca tgcaagattg agaaactgta
     1201 tttccaggta aagtactttt atggatgata tgaaagagct gaactgtgaa tccagaacta
     1261 atatcctagg actgtttttc ctagcgggag gtaaataaaa catgacagat gttccaatca
     1321 agtatcgctt gattaagaaa gaaaagcaca caggtgcacg tttgggcgaa atcattaccc
     1381 cacacgggac ctttccaact ccgatgttta tgccagttgg aactcaggca acggtaaaaa
     1441 ccatgtcgcc cgaagaattg aaggcgatgg gttcaggaat tatcctatct aatacctatc
     1501 atttgtggct tcgtccaggt gacgaattgg tggcgcgtgc aggcggtttg cacaaattta
     1561 tgaattggga tcaaccgatt ctgactgact caggtggttt ccaggtttat tctttggcgg
     1621 atagtcgcaa tatctcagaa gaaggtgtga ccttcaaaaa ccatctcaat ggtagtaaga
     1681 tgttcctgtc acctgaaaaa gctatctcta ttcagaacaa tcttggctct gacattatga
     1741 tgagctttga tgagtgtccg cagttctacc agccttatga ctatgtaaaa aaatccatcg
     1801 aaagaacaag tcgttgggca gaacgtggtc tcaaggctca ccgccgtccg catgaccaag
     1861 gtttatttgg tattgtccaa ggtgctggtt ttgaagatct tcgtcgccaa tcagcccatg
     1921 atttggtcag catggatttt ccaggatact ctattggagg attggctgtt ggtgaaacac
     1981 acgatgaaat gaatgcagta ctggacttca cgacccctct tttaccggag aataaaccac
     2041 gctatctcat gggcgttgga gcaccagata gcttgattga tggcgtaatt cgtggggttg
     2101 atatgtttga ctgtgtatta ccaacacgca ttgcgcgtaa tggaacctgt atgaccagtc
     2161 gtggacgtct tgtggtgaaa aatgctcagt tcgctgagga ctttacaccg ttagatccag
     2221 agtgcgattg ctacacatgt aaaaactata cacgtgcgta tctccgccat ttacttaagg
     2281 ccgacgagac atttggtatt cgtttgacaa gttatcacaa cctctacttc ttgattaatt
     2341 tgatgaaaaa tgtccgtcaa gcaatcatgg atgacaacct tttggaattc cgtcaagact
     2401 ttatggaaaa atacggttac ggtaaaaacg gacgtaattt ttagataaaa cagccttctt
     2461 tgtggactta gccttgttct catactcaat gaaaatcaaa atcaaactag gcgacgcaga
     2521 tgcagataga actgaagttc atcaaatcaa gtcaacaacg tctgattttg attttcgaag
     2581 agtatcagtt acaccttctc aagatttggt tagatacaaa taaataaagc atacgattat
     2641 gaaacccctc ggtaacataa tcgtatgctt ttattttaat acttggcttc acagcacttc
     2701 tattttaatg ttctttgaag attaaaatga acagttgtca acgagctttg atgagtgaaa
     2761 aaacgattgg tccccattac ccttcaaaat acagtaattc tttcggagtc tttgtgaata
     2821 cttcaaagcc atttttggta acgtaaccac aatcctcgat acgaacacca acttttccag
     2881 ggatgtaaat acctggttcg acagagaaac acataccttc ttcgatgact aaatcattgc
     2941 cttccataat tgatgggaat tcatggacac tcattcccaa accatgaccg agtcggtggt
     3001 tgaagtactc gccataacct gctttttcaa tcacagagcg agcggcatag tcaatctcac
     3061 cagcagtcac accaggctta atcatattga ccgcggccat gtgagcctcc agagtcaagt
     3121 tgtaaatatc ttttttaaac tgatcaggtt taccgacagc gacagtccgt gtcatatcag
     3181 acgtatagcc caaagcttcc acgcctaggt cgaacaatag cagagcgtta ttctcgattt
     3241 tatttgttga gggaataccg tgtggatttg cggcattgtc gcctgttaag accatggtgt
     3301 caaagctcat ttttgagatg ccttgtttct tcatttcaaa ttcaatctga gcaataatat
     3361 cagtttcagt tacgttgagg gagatattgt taaaaccaac ctgcatagcc ttatccgcaa
     3421 actctccagc aaccagcatt ttttcaattt catcacgcga cttgatcaat ttcatagtat
     3481 taatcagagg tgtaatgtct gaaaatggtt ggctaaagat agactgaaga ccatggtagc
     3541 gggttaaatt caaattatca aattctgcac agattactga gaaagatttc tgtggcaatt
     3601 tgctcttgat aatctgccat ggattttctg agtccatata gccggctaca ggaaagtcaa
     3661 cagttgcaac tgcacgttct acatccaagg cagggagaaa gagtagtgag tcgtggtcag
     3721 gcataacaaa gagcatcatg tggcgctcat gaggatcgct atgatagcca gtcagataat
     3781 agatgctaac tggatcagag aagatggcca agtccatttt gttggcttct aagtaggatt
     3841 taacatgatg taattttgac atagtggaga cctttctagc atgtagtggt aaaaaaaagt
     3901 gaaattttcg tttattatat agactattgt ttcaaaaaat gaaaaaaatt gcaagtatta
     3961 tcaaaaaaat agagtttatc tcttgaaagt ggtttcaaaa agtgatacac tatacttgag
     4021 tgaaagcgta attttcatag aactagaact attgaaaggg agtacataac gtatgttaaa
     4081 cactgacgat acggtaacga tttatgacgt tgcccgcgaa gcaggtgtat ccatggcgac
     4141 agtatcgcgc gtggtaaatg ggaataaaaa cgtaaaggaa aatactcgta aaaaagtatt
     4201 agaagtcatc gaccgtttgg attatcgtcc gaatgctgtt gcacgtggct tggccagcaa
     4261 gaagactacc actgtggggg ttgtgattcc aaatattgct aatgcttatt ttgcaacctt
     4321 ggccaaaggt atcgatgata ttgccgatat gtacaaatac aatatcgtct tagcaaacag
     4381 tgatgaaaat gatgagaaag aaatcaatgt ggtgaatacc ctattctcaa aacaggtgga
     4441 cggaatcatt ttcatgggct atcatttgac agacaagatt cgtgcggagt tttcacgctc
     4501 acgtacaccg attgttttag ctggtaccgt ggacttggag caccaattac ctagcgtcaa
     4561 tattgactac gctgccgcta gtgttgatgc agtcaatcta ttagctaaga acaacaagaa
     4621 aattgccttt gtatcagggc cgcttgtaga tgacatcaac ggtaaagttc gttttgcagg
     4681 ctacaaacaa ggcttgaagg acaacggaat cgagtttaac gaaggattgg tttttgaatc
     4741 caagtataaa tacgaggaag gctacgctct agcagaacgt attttgaatg ccggagcaac
     4801 tgcagcttat gttgcagaag atgagattgc tgctggttta ttgaatggtg tcagtgatat
     4861 gggcgtcaag gttccagaag actttgaaat cattacaagt gatgattccc tagtgactaa
     4921 gtttacccgt ccaaacctga cctctatcaa tcagccacta tatgatattg gtgcaattgc
     4981 tatgcgcatg ctcaccaaaa tcatgcataa ggaagagttg gaaaaccgtg aggtagttct
     5041 taaccacgga atcaaagtac ggaaatcaac taagtgataa atatgggagt agccggataa
     5101 gatatcttat tactccgcac cctcactcta tcaaacatta taatcatttg gtttatcttt
     5161 tattacttcg acgagcgagg aaatatttcc aacctagaac agcctttagg ctgttctagc
     5221 aacttgtagc tattgaggca aacttgcttc gacttatctc cagccttcaa aggttcggag
     5281 aacctttgaa gcgagtacta aaagcaaaca aaatggctat actaaccgaa aactaaaaga
     5341 ttataaaaaa taaatataaa gccctcgcta gatgtataag cttagcgagg gctttgtggt
     5401 tgattttaag tctgtcacac tctgtaagtt gtgcgagatg ttgatgcgaa tctagagaag
     5461 cagtactggg cttgttctca tcctttagaa ctgtatcagt taggtgaatt ataatttacg
     5521 tttacgtgta aacaacgcaa ttgcaagccc gataatacca cttgtcaata accataaact
     5581 tcctttctca ccagttactg gtaagatttg cttagcagtt tcggttttaa atgttgaagt
     5641 ctcctgcttg tctgtttttt cagttgttgg tttagtgttt gaattttctc cgactgtacc
     5701 attttggttg ttcccattaa caggtatctg ttttttagag gtttcgatat tttccttctc
     5761 tttattagct atttgccttt caggtgttac ggcattgtcc ttctcattaa caggcatctg
     5821 tttttcagag gtttcaacat tatcgttctc tttattagcc atttgttctt gaggtgttac
     5881 gaccatgtcg ttcacaatgc ttggcatctg tttttcagag attacaactt catcgttctc
     5941 tttattagcc atttgttctt gaggtgttac gaccatgtcg ttcacaatgc ttggcatctg
     6001 tttttcagag attacaacct tatcgttctc tttattagtc atttgctttt caggtgttac
     6061 ggcattttcg ttcacaacac tcggcatttg tttttcagag gtttcaatat tatc
//
LOCUS       DBNFEG010000128         6085 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.128, whole genome shotgun sequence.
ACCESSION   DBNFEG010000128 DBNFEG010000000
VERSION     DBNFEG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.128"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1306
                     /locus_tag="AC1PNG_001631"
     CDS             <1..1306
                     /locus_tag="AC1PNG_001631"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIP6702122.1"
                     /translation="KVFRDPVHNYVHVDHELIYKLINTKEFQRLRRIKQLGTTSYTFH
                     GGEHSRFSHCLGAYEIARRITQKFEDKYPQIWDTNESLLTMVAALLHDVGHGAYSHTF
                     ERLFDTDHEEMTCAIITSPETEINALLKQVSPEFPDKVASVIKHTYPNKQVVQLISSQ
                     IDVDRMDYLLRDSYFTGANYGEFDLTRILRVIRPTENGIAFKESGMHAVEDYVLSRYQ
                     MYMQVYFHPASRSMEVLLQNLLNRAKLLYTSEQEFFARTSPRLLPFFEHRIRLTDYLS
                     LDDGVMNTYFQSWIDGPDRILSDLAQRYINRKVLKSITFKAEEEEALDRLRSLVADVG
                     FDPEYYTAIHHNFDLPYDIYRPNAEKKRTQIEIYRKDETLVELSSLSPIVHSLSGTIH
                     GDSRFYFPKEMLEETGIFAPQIADFNSHIHNDHFIIGESNEH"
     gene            1296..2105
                     /gene="yidA"
                     /locus_tag="AC1PNG_001632"
     CDS             1296..2105
                     /gene="yidA"
                     /locus_tag="AC1PNG_001632"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HIP6702123.1"
                     /translation="MSIKLVAIDIDGTLLNSQHQITAEVHSAIQESKKAGVKIVIATG
                     RPISGVQTILEELNLTEAGDYVITFNGGLVQDAATGEDIVKDTLTYEDYLDIESAARK
                     LKLPMHASTKEGIYTANRNIGKYTIYESTLVSAPIFYRTPEEMADKEIIKTMMVDEPE
                     ILDAAIPLLPTSLTEKYNVAKSAPFYLEITPKTVNKGQAIIHLAEKLGLTMDQTMAIG
                     DQENDRPMLEVVGNPVVMENGNPELKKIAKYITKSNDESGVAHALREWVLK"
     gene            complement(2159..2911)
                     /gene="tpiA"
                     /locus_tag="AC1PNG_001633"
     CDS             complement(2159..2911)
                     /gene="tpiA"
                     /locus_tag="AC1PNG_001633"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIP6702124.1"
                     /translation="MSRKPIIAGNWKMNKNPQEAQAFVEAIAGKLPAGDKIEAAIAAP
                     AVDLNALLWFAKDSELKVAAQNCYFEDAGAFTGETSPKVLAEMGVNYVVIGHSERRDY
                     FHETDEDINKKAHAIFRNGLTPIICCGESLETYEAGKAVEFVGAQVSAALKDLTAEQV
                     ASLVIAYEPIWAIGTGKSATKDDAQNMCKAVRDVVAADFGQEVADKVRVQYGGSVNPS
                     NVAEYMACPDVDGALVGGASLEAESFLALLNF"
     gene            complement(3086..4282)
                     /gene="tuf"
                     /locus_tag="AC1PNG_001634"
     CDS             complement(3086..4282)
                     /gene="tuf"
                     /locus_tag="AC1PNG_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIP6702125.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEIEIVGLQEEKSKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVISKPGSINPHTKFKGEVYILTKEEGGRHTPFFDNYRPQ
                     FYFRTTDVTGSIKLPEGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(4638..5609)
                     /locus_tag="AC1PNG_001635"
     CDS             complement(4638..5609)
                     /locus_tag="AC1PNG_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti sigma factor C-terminal domain-containing
                     protein"
                     /protein_id="HIP6702126.1"
                     /translation="MTMKTFEEVTKKSKRKNLWKTIGLSSLIGFILMLIGVQTVKIIV
                     GAREGRENWKQIILMEISYPNIKHDGIKIQSTSYVSGKLYCNLYKDLAGVPMAFGQHE
                     IYYDLFDRHYDFAQHIPGNTFKYLYDFTTNTKVPVFYNRKKSVWSAQDIANELHYLKD
                     MSGQLVEVAISFDKKYSLAEIKQMIPSNLKQNWYWIGTEGKQNTHYYRPEQLLGLDPE
                     DIQLVFSDMPGDYMTGMDFMKEFLESGDQERAQYLDIPVDDLQAFVDKFGGTDFTKQE
                     EIDQLEFAGIILTGKAEDFAQLEGKDWIYSSSIGASIPIQPYYQLHH"
     gene            complement(5590..>6085)
                     /locus_tag="AC1PNG_001636"
     CDS             complement(5590..>6085)
                     /locus_tag="AC1PNG_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIP6702127.1"
                     /translation="KLDQYEKEIIGIAEEISYYLQKSGAGPADSQDIAQDVLVKLLES
                     DIVLPFEKLRAWLYRSAVRAYIDKYRRDKRYHEILQKEFFRREDLLVYDQEGYEELYQ
                     AVADLPEKYQQVIDLYYFQDMSVKEVAHILGTGQSWVKIILYRGRKQLRKILEERGYD
                     YEDF"
BASE COUNT         1794 a         1350 c         1147 g         1794 t
ORIGIN      
        1 aaaagtattt cgcgatccag ttcataacta tgtacatgtt gatcatgagc tgatttataa
       61 acttatcaat acaaaagaat ttcagcgact acgccgcatc aagcaactag ggactacttc
      121 ctataccttc catggtggcg agcatagccg cttttcgcac tgcctcggcg cctacgaaat
      181 cgccagacgg attactcaaa aatttgaaga caagtatcca caaatctggg atacaaatga
      241 gtccctactg accatggttg ctgcgctact ccatgatgtc ggacacggag cctattctca
      301 taccttcgag cgtctatttg atacagacca tgaggaaatg acctgtgcaa ttattactag
      361 tccagaaacg gaaatcaatg ctctgttgaa acaagtttca cctgagtttc cagacaaggt
      421 tgctagtgtt atcaaacaca cctatccaaa taaacaggtc gttcaactca tttcgagcca
      481 gattgatgtt gaccggatgg attatctatt acgagattcc tacttcacag gtgcgaacta
      541 tggtgaattt gacctaacta ggatattacg tgtcattcgc ccgacagaaa atggaattgc
      601 cttcaaagaa tctggtatgc atgcagtaga agactacgtt ctcagccgct accagatgta
      661 catgcaagtc tattttcatc cagcaagccg ctctatggaa gtcctattgc agaatttact
      721 caatcgtgcc aaactactat atactagtga acaggagttt tttgcacgaa cttcacctcg
      781 gctattacct ttttttgagc atcgtatccg tctaaccgac tacctcagtc ttgatgatgg
      841 agttatgaat acttactttc aatcatggat agatggacca gatcgtatat tatctgactt
      901 agcccagcgc tatatcaata ggaaagtgct caaatccatc actttcaagg ccgaagaaga
      961 ggaagctttg gaccgcctgc gtagcctggt cgcagacgtc gggtttgacc cagaatacta
     1021 tactgctatt caccacaact ttgatttgcc gtatgacatt taccgaccga atgcagaaaa
     1081 gaaacggact caaattgaga tttatcgcaa agatgaaacc ttggtagaac tatcctctct
     1141 gtcaccaatt gtccattcct tatcaggcac aatccatggc gatagccgct tctatttccc
     1201 gaaagaaatg ttagaagaaa caggaatttt cgcacctcaa atagctgatt ttaacagcca
     1261 catacataac gatcatttta taattggaga atcaaatgag cattaaactt gtcgccatcg
     1321 atattgatgg tacactacta aatagtcaac accaaattac agctgaagta cactcagcaa
     1381 tccaagaatc aaaaaaagca ggcgttaaaa ttgttattgc tactggtcgc cccatttccg
     1441 gtgttcaaac aattttagaa gaactcaatc ttacagaagc aggtgactac gtcatcactt
     1501 ttaacggtgg cttagtccaa gatgcagcaa ctggtgagga tattgtcaaa gataccttga
     1561 cttatgagga ctatctcgac atcgaatctg ctgcccgtaa actcaagctc ccaatgcatg
     1621 ccagcaccaa ggaaggcatc tatactgcta accgtaacat cggtaaatac accatttacg
     1681 aatcaacact agtaagtgca cccattttct atcgcactcc tgaagaaatg gctgacaagg
     1741 aaatcatcaa aacaatgatg gttgatgagc cagaaattct tgatgctgct attccacttc
     1801 taccgaccag cttgactgag aagtacaacg tagcaaaatc agcaccattt tatttggaaa
     1861 tcacccctaa aactgttaac aagggacaag ctatcattca tctagctgag aagcttggac
     1921 taactatgga tcaaactatg gccatcggtg accaagaaaa tgaccgtcca atgttggaag
     1981 ttgttggtaa tcctgttgtc atggaaaacg gaaatcctga attgaagaaa atcgccaaat
     2041 acattacgaa gtcaaatgat gagagcggtg tagcccacgc tcttagagaa tgggtactga
     2101 aatagcctct taaaatatca aaaaaagcat cgaattcaaa atccgatgct ttttgttatt
     2161 agaagttcaa caaagccaag aagctttctg cttcaagtga tgcaccacca acaagcgcac
     2221 cgtcaacgtc tggacatgcc atgtattcag caacgtttga tgggtttaca gaaccaccgt
     2281 attgaacgcg aactttgtct gcaacttctt gaccgaaatc agcagcaaca acgtcacgga
     2341 cagctttaca catgttttgt gcatcatctt tagtagctga tttacctgta ccgattgccc
     2401 agattggctc ataagcaata acaagtgatg ctacttgttc agcagtcaag tctttcaaag
     2461 ctgcagaaac ttgtgcacct acaaattcta ctgctttacc agcttcgtaa gtttcaagtg
     2521 actcaccaca acagatgatt ggagtcaaac cgttacggaa gattgcatga gcttttttat
     2581 taatatcttc atcagtttcg tggaagtaat cacgacgctc tgagtgaccg ataacaacat
     2641 agttaacacc catttcagcc aatacttttg gagatgtttc accagtgaag gcaccagcat
     2701 cttcaaagta gcagttttga gcagcaactt tcaattcaga atctttagcg aaccacaaga
     2761 gagcgttcaa atctacagct ggagctgcga tagctgcttc aattttgtcg cctgcaggca
     2821 attttcctgc aattgcctca acgaaagctt gtgcttcttg agggttttta ttcattttcc
     2881 agttaccggc aatgattggt ttacgtgaca tttcacttac ctcttctata tttattgtac
     2941 aaggtacatt atagcataaa cttagctaag attaaagaat ttctgtgcat tttatgaata
     3001 cttacagaaa aactctctac ccgaaagtag agagttacaa ttaagttgtc tgactataat
     3061 tgttatttct gggaactcaa tcgaattaag cttcgatttc tgtaaccata cctgaaccaa
     3121 cagtacgtcc accttcacgg atagagaaag tagtaccttg ttcaacggcg attgggtgga
     3181 tcaattcaac gtcgatagta acgttatcac caggcattac catttcagta ccttctggca
     3241 atttgattga accagttacg tcagttgtac ggaagtagaa ctgtggacgg tagttgtcga
     3301 agaatggagt gtgacgtcca ccttcttctt tagtaaggat gtaaacttca cctttgaatt
     3361 tagtgtgtgg gttgatagaa cctggtttag agataacttg accacgttcg atttcatcac
     3421 gttgtacacc acgaagaagc acaccaacgt tatcgccggc aagaccttcg tcaagttgtt
     3481 tacggaacat ttcaacacca gtaacaactg ctttagattt ttcttcttga agaccaacga
     3541 tttcgatttc gtcgttgaca cgaacagtac cacggtcgat acgtcctgaa gctacagtac
     3601 cacgaccagt gattgagaat acgtcctcga ctggaagcaa caatggtttg tcagtgtcgc
     3661 gttctggttc tggaatgtac tcatcaacag tgttcatcaa ttccataacg atgtcttcgt
     3721 acttagagtc accttcaaga gctttaagag ctgaaccttg gataactgga agatcatcac
     3781 ctgggaaatc gtattctgaa agaaggtcac ggatttccat ttcaaccaac tcaagcaatt
     3841 cttcatcgtc aaccaagtca actttgttca tgaagacgat aaggtgttta acaccaacct
     3901 gacgtgaaag aaggatgtgc tcacgagttt gtggcattgg accgtcagtt gaagctacta
     3961 caaggatcgc accgtccatc tgagcagcac cagtgatcat gtttttaacg tagtccgcgt
     4021 gtcctggagc gtcgatgtga gcatagtgac gtttttcagt ttcgtactca acgtgtgcag
     4081 tgttgatagt gataccgcgc tcgcgctctt ctggagcagc atcgatagac gcatagtctt
     4141 taggttggtt aactgatgaa ggcaagcgac gtgccaatac agttgtgata gctgctgtca
     4201 aagtagtttt accgtggtca acgtgtccaa ttgtaccaat gttaacgtgt ggtttactac
     4261 gatcgtattt ttcttttgcc attttggtaa aagcctccaa taaaatatat tttatagata
     4321 gacagtaggc aatacagtct aactttacct tactattcta tcaaattgta gcagaattgc
     4381 aagcattttc tatcgttttt tattttatcc attttctgca agggctatta gcttcgctta
     4441 taactttttt tatcatccca ttttaaccta ttactcaact aaaaacacct cactgccata
     4501 ggaatattga tgttaccata gctacaaggt gccacaaata cgagactact gcgacagacc
     4561 tttctccatt cagtcatcct acgactccct gacaagcctc ttgctatagg tattgaaaat
     4621 atattgacta ttttcactta atgatgcagt tgataataag gctgaatagg aatggacgcg
     4681 ccaatactag aagaataaat ccagtccttg ccttccaact gagcaaagtc ttctgctttt
     4741 cctgttagga taatgcctgc gaactccaac tgatcaatct cttcttgttt ggtaaagtca
     4801 gtgccaccaa acttatcgac aaaggcctgc aagtcatcca caggtatgtc cagatactgt
     4861 gctcgctcct gatctcccga ttctaagaac tctttcataa agtccatacc tgtcatatag
     4921 tctccaggca tatccgaaaa gactagttga atatcctctg gatctaaccc caaaagctgc
     4981 tctggacgat agtaatgggt attctgcttc ccttctgtcc ctatccaata ccaattttgc
     5041 ttgagattgc tgggaatcat ttgcttgatt tctgccaaac tgtacttctt atcaaaggaa
     5101 atagccactt ctactagctg cccactcatg tctttaagat aatgcaattc attggcaatg
     5161 tcttgagcac tccaaacaga cttttttcta ttgtaaaaaa caggtacctt ggtattggtt
     5221 gtgaaatcat atagatattt gaaggtattc ccaggtatat gctgagcaaa atcatagtga
     5281 cgatcaaaca ggtcatagta aatttcgtgc tgaccaaaag ccataggaac accagctaga
     5341 tccttatata aattgcagta aagtttccca gaaacataac tcgtgctttg gattttaata
     5401 ccgtcatgtt ttatatttgg ataagagatt tccatcaaaa taatttgttt ccaattctca
     5461 cgaccttctc gtgcaccgac gatgattttt actgtttgaa cgccaataag cattaagatg
     5521 aagccgatga gcgatgatag ccctattgtc ttccataaat ttttccgctt actctttttt
     5581 gtcacctctt caaaagtctt catagtcata tcctctctct tctagtattt tcctcaactg
     5641 cttgcgtccg cggtaaagga taattttcac ccaactttga ccagtcccta agatatgggc
     5701 aacttctttg acactcatat cttgaaaata atagaggtca atcacttgct ggtatttttc
     5761 tggcaggtct gcaactgctt gatagagttc ctcataacct tcttggtcat agaccaaaag
     5821 gtcttctctt ctaaaaaact ccttttgtaa aatttcatgg tagcgcttgt ctcgcctgta
     5881 tttgtcaatg taagcccgaa ctgcagatcg gtaaagccaa gctcttagtt tttcgaaagg
     5941 aaggacaata tcagattcaa gcaatttgac tagaacatct tgggcaatat cctgactgtc
     6001 agctgggccg gctcctgatt tttgcaaata gtaggaaatc tcctctgcaa taccaataat
     6061 ttctttttca tattgatcta gtttg
//
LOCUS       DBNFEG010000129         6082 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.129, whole genome shotgun sequence.
ACCESSION   DBNFEG010000129 DBNFEG010000000
VERSION     DBNFEG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.129"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..655)
                     /locus_tag="AC1PNG_001637"
     CDS             complement(<1..655)
                     /locus_tag="AC1PNG_001637"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HIP6702128.1"
                     /translation="MQYKHLLFDLDHTLLDFSRGEEVALTQFLTAMEVEDIQAFKEVY
                     RPLNQGMWKDLEKGNITKKELINTRFSRTFAHFGRQVDGREMALRYQEFIGRQGQIFT
                     GADKLLQELTHRGYQIYAATNGVTYIQENRLLHSPIQSYFKEVFISEQMGTQKPAADF
                     YEKIAEQISGFQFDQALMIGDSLTADIQGGNNAGMDTVWYNPTHMINNSKAVPTYTIH
                     "
     gene            complement(934..1674)
                     /locus_tag="AC1PNG_001638"
     CDS             complement(934..1674)
                     /locus_tag="AC1PNG_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor 2"
                     /protein_id="HIP6702129.1"
                     /translation="MSKRKIISSLFLSATVLGGLLFTQTPTVKAIELEDAQTMSTSSA
                     QAPIFSPGELIQETNYNDLVQKSVSIEDFSTNSNNEFTWKVKFDPTHWNFKHDKGGYY
                     FIIPEGMKLKKLVDKHTEKDLLTNFPENVNDSKNDSYSQYRHFKKGERTYWDRDFDSQ
                     WGWSAGRASNDKINQWKDENAFSDIYYIDSPRHAGPVTYELEAEVTDQNKTSFPLVAV
                     MKNFYARTSYLSEPTSLAGLDLKVEWPK"
     gene            complement(2116..4395)
                     /gene="pcrA"
                     /locus_tag="AC1PNG_001639"
     CDS             complement(2116..4395)
                     /gene="pcrA"
                     /locus_tag="AC1PNG_001639"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HIP6702130.1"
                     /translation="MNPLLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKMVNPWNILAITFTNKAAREMKERAYALNPATQACLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRSLMKRILKNLNLDPKKWSERSILGTISNAKNDLIDDKAFEAQA
                     GDLYTQIVAKCYTAYQKELRQSEAVDFDDLIMLTLRLFDQNPEVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPESKVVL
                     LEENYRSTKNVLQAANEVIENNRNRRPKKLWTQNVQGDLITYYRARDENDEAIFVAGQ
                     IDQLHREGKAYKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDVISY
                     LNIIANPSDNISYERIVNEPKRGVGPGTVDKLRDFATSHSMSLLEASQDIMLSPVKGK
                     AAQAVFDLANLLYNLRNQLDNLTVTQVVEAVLNQTGYIDALAVQNTLEANARIENIQE
                     FLSVTKNFDEKDVEEEETGLDKLTRFLLDLALIADTDDGDTESSEVTLMTLHAAKGLE
                     FPVVFLIGMEENVFPLSRAAEEEDELEEERRLAYVGITRAEQTLYLTNANSRLLFGRT
                     NYNQPSRFIREISSDLLDYQGLARPANTSFKASYVNGRATQFGQGMSLQQALQSRKAS
                     VQPSAKLGGSMPFASGNKSPGSGTNWSVGDIAVHKKWGRGTVLEVSGSGSNQELKINF
                     PEMGLKKVLASLAPISKEE"
     gene            complement(4685..6052)
                     /gene="nox"
                     /locus_tag="AC1PNG_001640"
     CDS             complement(4685..6052)
                     /gene="nox"
                     /locus_tag="AC1PNG_001640"
                     /EC_number="1.6.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H2O-forming NADH oxidase"
                     /protein_id="HIP6702131.1"
                     /translation="MAKIVVVGANHAGTAAIKTMLTNYGQENEIVVFDQNSNISFLGC
                     GMALWIGEQIGGPEGLFYSNKEELESLGATVYMESPVTNIDYDAKTVTALVNGQEHVE
                     SFEKLLFATGSQPILPPIKGAEIKEGSLEFEATLENLQFVKLYQNSADVIEKLKNKDI
                     NRVAVVGAGYIGVELAEAFQRKGKEVVLIDVVDTCLAGYYDRDLSDIMAKNLEEHGIK
                     LAFGETVKEVAGNGKVEKIITDKNEYDVDMVILAVGFRPNTALAGEGIERFRNGAFLV
                     NKRQETSIPGVYAIGDCATIYDNATGDTSYIALASNAVRTGIVAAHNICGTDLEGIGV
                     QGSNGISIYGLNLVSTGLTLEKATRLGLNAAVTEVTDNQKPEFMEHGNFPVTIKIVYD
                     KDSRRILGAQMAAREDISLGIHLFSLAIQEGVTIEKLALTDLFFLPHFNKPYNYITVA
                     ALGAE"
BASE COUNT         1661 a         1322 c         1233 g         1866 t
ORIGIN      
        1 tatgaatggt ataggttgga actgccttac tattatttat catatgagtc ggattatacc
       61 aaacagtatc catgccagca ttattgccac cttgaatatc tgctgtcaaa ctgtctccaa
      121 tcattaaggc ttggtcaaac tgaaaacctg aaatctgctc tgctattttt tcataaaaat
      181 cagccgcagg cttttgcgtc cccatctgct cagaaataaa tacttcttta aaatacgatt
      241 gaatcggtga gtgaagcaag cgattttcct gtatataggt cacgccgttg gtcgctgcat
      301 aaatctggta gcctcggtgt gtcaattcct gcaaaagctt atctgcacct gtaaaaatct
      361 gtccctgccg cccaataaat tcttggtagc gcaaggccat ctcccttcca tcaacttgcc
      421 gaccaaagtg ggcaaaggtc cgtgaaaagc gggtgtttat tagctctttc tttgtaatgt
      481 tccctttttc caaatccttc cacatgccct ggttgagcgg tcgatagact tctttaaaag
      541 cttgaatatc ttcaacctcc atagctgtca aaaactgggt taaggctact tcttctccac
      601 ggctaaaatc aagaagagta tggtcaagat caaagagtaa gtgtttgtat tgcaagatga
      661 taggtccttt ctaagcagaa actactgaaa cgatgtcagg cagtctatct aaagtatacc
      721 acagtttcta aaaaaaatag ctccgtgtcc taggctctat tatactgttt ttcagatttc
      781 agtagctctg gacgtacggg tttcctcatc atattatgca agcgctagtt tattcaagta
      841 aagagaagag gactcgcaga aaaaggcaat cgtagtagcc ttaactacac acgattgcca
      901 ctggttggtt ttgttctgca aattgtcagt cacttatttc ggccattcaa ctttcaaatc
      961 aagtcctgca agcgaagttg gttcactcaa gtatgatgtt ctagcataga agtttttcat
     1021 taccgctacc agagggaagg aagttttatt ttgatcagtt acttctgcct ccaattcata
     1081 agttactgga cctgcatggc gaggactatc gatataatag atgtcagaaa atgcattttc
     1141 atctttccac tgatttatct tatcattact tgctcgtcca gctgaccatc cccactgact
     1201 atcgaagtca cgatcccaat atgttctttc gcctttttta aagtggcggt actgactgta
     1261 tgagtcgttc ttactgtcat taacgttttc cgggaagtta gttaataagt ccttttccgt
     1321 atgtttgtca acaagtttct tcaatttcat accttcagga ataataaaat aatatcctcc
     1381 cttatcatgc ttaaagttcc aatgtgtagg atcgaacttt actttccatg taaactcatt
     1441 attgctattt gtcgaaaaat cctcaataga tactgacttt tggaccaagt cgttatagtt
     1501 agtctcttga ataagttccc caggagaaaa aatcggtgct tgtgcgctgc tagtactcat
     1561 ggtttgtgca tcctctaact caattgcttt tacggttggt gtctgtgtaa acagcaatcc
     1621 tcccaatacg gtagcactta aaaataaact tgatataatt ttacgtttgc tcataagagt
     1681 ctccttcttt tatttgatct atttttgcgc tagctacaaa aatattggca agctaatcgt
     1741 cccatttcta ggctatccct tccttactaa gatgagagtc tctagcaaat aaaagacttt
     1801 taactactat cagtataaca atacaagtag tattttctct tggagctatc cgtttttttc
     1861 tatatttgtg gtttagcata tggatggtaa gacacactct aaaaacatcc gattcaaaat
     1921 tcaaaatttg cttatctaga tgtcatcaaa gcgtgcaaga ttgtttcgat gctttaagca
     1981 gtatacaaaa aatcacctca aaacagtcag gtcaaaactc attttgaggt gtcatatatc
     2041 gttctaaaaa taatattatg aagacgtcgc aagtcttgta aaatgcgatg tttcctcttt
     2101 acttttgatt catccctact cttccttact aatcggtgct aagcttgcca agactttttt
     2161 gaggcccatt tctgggaaat tgattttgag ttcttggttg ctgcctgaac ctgatacttc
     2221 aaggactgtc ccacgtcccc atttcttgtg gactgcgatg tcaccaactg accagttggt
     2281 gcctgagcca ggcgatttgt tgcctgatgc gaaaggcata ctgcctccga gcttggcaga
     2341 tggttgtacg gatgcttttc gactttgtag ggcttgttgc aagctcatgc cttgtccaaa
     2401 ctgtgtcgct ctgccattga cataagaagc cttaaagcta gtattagctg gacgagcgag
     2461 gccttgataa tcaagcaagt cactggaaat ttctcgaata aagcggcttg gttggttgta
     2521 gttggtgcga ccgaaaagga gtcgagaatt ggcattggtc aggtagagag tctgctcagc
     2581 acgggtgatg cctacgtatg ccaaacgacg ttcttcctcc aattcgtcct cttcttctgc
     2641 tgcacgacta agtgggaaga cattttcctc cataccaatc aagaagacaa ctggaaattc
     2701 cagacctttg gctgcgtgga gggtcataag agtcacttct gaagattctg tatcgccatc
     2761 atctgtgtca gcaatcaggg ccaaatcaag caggaagcgt gttaatttat ctagacctgt
     2821 ctcttcttcc tcaacatcct tttcatcaaa gtttttggta acagaaagga attcctggat
     2881 gttttcaatg cgggcatttg cttccagggt attttgtact gcaagggcat cgatgtagcc
     2941 tgtttggttc aatactgcct cgactacttg ggtaaccgtc aagttgtcta attgattgcg
     3001 aagattatag aggagattag ctaagtcaaa gactgcctgt gctgccttgc ccttgactgg
     3061 tgacaacata atatcctgag aagcttctag caaggacatg ctgtggctgg tcgcaaaatc
     3121 acggagtttg tcaaccgtac cagggccaac gccacgtttt ggctcattga caatgcgttc
     3181 ataagagata ttatccgatg gatttgcgat gatattcaaa taagaaatca catctcgaat
     3241 ttccttacgg ctgtagaact tggtgccgcc aaccatggta taagggatat tagatttgag
     3301 cagggcttct tcaatggtac gggattgggc attggtacgg tagagaactg caaaatcctt
     3361 ataggcctta ccttctcggt gcaactggtc aatctgccct gcgacaaaaa tcgcctcgtc
     3421 attctcatct ctggcacgat agtaggtaat caaatcccct tggacattct gtgtccagag
     3481 ttttttggga cgtcgattgc ggttgttttc gatgacctca ttggcagcct gcaagacgtt
     3541 cttggtcgaa cgatagtttt cctccaaaag cacaaccttg ctctctggat aatccttttc
     3601 aaagtccagg atattttgca tatccgcacc acgccaacca tagatagact ggtccgcatc
     3661 accgaccacg cagatattct tgaaacgtga tgccaggagc ttgaccaatt ggtactgggc
     3721 atggttggta tcctgatact catcgacatg gatatactgg aatttctgct ggtaataggt
     3781 caggacctct ggattttggt caaaaaggcg gagcgtcaac atgatcaaat catcaaagtc
     3841 aacggcctct gactgtctta gttccttttg gtaagccgtg tagcatttag caacaatctg
     3901 cgtgtatagg tcgcctgcct gggcttcaaa cgccttgtca tcaatcaagt cgtttttggc
     3961 attagaaatg gttcccagga tagaacgttc actccacttt ttcggatcta gattgagatt
     4021 ttttaaaatc cgtttcatga gactgcgttg ctcacctgga tcgacaatcg taaaattgcg
     4081 attgtaaccg atatggtcag catcccgacg caaaatccgc acacacatgg agtggaaggt
     4141 agctatcaaa caggcttgcg tggcaggatt gagggcatag gcccgctcct tcatctcccg
     4201 tgccgccttg ttggtaaagg taatggctag gatattccaa ggattgacca ttttttcatc
     4261 aatcaaataa gcgatacggt gggtcaggac tcgcgtctta cccgaaccag cccctgccat
     4321 aatcaaaagc ggaccttccg tcgtctgtac cgcttctgcc tgtctgtcat tcattccgtt
     4381 taataatggg ttcattttct catactttcc tagaacatgt atttacaagt aattcacttt
     4441 caaataggaa atcgtttata gctaatcaag gtaatttttt gaggaaatac cgactgtatt
     4501 tttggaaaaa caaacgataa atagctgaaa aactaggctt agatgaaagt gctaaactgt
     4561 aaatacaggt tcattataat cataccctcc attataccaa aaattctctc gataggcagg
     4621 tgcaaaatga tggagaacag ccaaaaaagc ccagagtcat ctctgagctt ttgtattcaa
     4681 ttgattattc agcacccaag gcagctactg tgatgtagtt gtaaggtttg ttgaagtgtg
     4741 gcaagaagaa caagtctgtc aaggccaatt tttcgattgt tacaccttct tggattgcca
     4801 atgagaagag gtggataccc aatgagatat cttcacgagc agccatttgc gcaccaagga
     4861 tacgacgtga atctttatcg taaacaatct tgatagttac tgggaagtta ccgtgctcca
     4921 tgaattctgg tttttggttg tctgttactt cagtaacagc agcgttcaaa ccaagacgag
     4981 tagctttttc aagtgtcaaa cctgttgata caaggttaag accgtagata gagataccgt
     5041 tagaaccttg tacaccgata ccttcaaggt cagtgccaca gatgttgtga gctgctacga
     5101 taccagtacg tactgcgttt gaagccaaag cgatgtagct tgtgtcgcca gtagcgttgt
     5161 cgtagattgt tgcacagtca ccgatagcgt aaacgcctgg gattgaagtt tcttggcgtt
     5221 tgtttacaag gaaggcaccg ttacggaagc gctcgattcc ttcacctgca agcgcagtgt
     5281 ttggacggaa accaacagcc aagataacca tgtcaacatc gtattcgttc ttgtcagtga
     5341 tgattttctc aactttacca ttaccagcaa cttctttaac agtttcgccg aaggcaagtt
     5401 tgataccatg ctcttccaag tttttagcca taatatctga caagtcacgg tcatagtacc
     5461 cagccaaaca tgtatccaca acatcaataa gtactacttc tttaccttta cgttggaatg
     5521 cttccgcaag ctcaacaccg atgtaaccag caccaacaac tgcgacgcgg ttgatgtctt
     5581 tgtttttcaa tttttcaata acatctgctg agttttggta caatttaaca aattgcaagt
     5641 tttcaagagt tgcttcaaat tcaagcgaac cttctttgat ttcagctcct ttgattgggg
     5701 gtaagatagg ttgtgaacca gttgcgaaaa gaagtttttc aaatgattca acatgttctt
     5761 gaccatttac aagcgctgta actgttttgg catcatagtc aatgtttgta acaggtgact
     5821 ccatgtatac tgtcgcaccc aagctctcca actcttcttt gtttgagtag aaaagtcctt
     5881 caggaccgcc aatttgctca ccgatccaca aagccatacc acaacccaag aatgaaatat
     5941 ttgagttttg gtcaaataca acgatttcat tttcttgacc ataatttgtc aacatagttt
     6001 tgattgcggc agtaccagca tggttagcac cgacaacaac gattttagcc ataatgtaaa
     6061 atctcacttt cgaaaatata tt
//
LOCUS       DBNFEG010000130         6044 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.130, whole genome shotgun sequence.
ACCESSION   DBNFEG010000130 DBNFEG010000000
VERSION     DBNFEG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.130"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(108..1910)
                     /gene="pepF"
                     /locus_tag="AC1PNG_001641"
     CDS             complement(108..1910)
                     /gene="pepF"
                     /locus_tag="AC1PNG_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HIP6702132.1"
                     /translation="MSKQRYEIEEKYQWDLTTIFPTDDAWEAELADLQAETEKTKEFA
                     GHLLDSAKSLLEISETQLSLMRRIEKLYVYASMKNDQDTREGKYQEFQAKAMGLYAAF
                     SQAFAFYEPEFMAITEEQLEAFKAEEPTLVQYSHQFEKLLAAKDHVLSQEVEEVLAAT
                     SEIFESPAETFSVLDNASLRFPEIADEDGHLVPLSHGNYIHFMESKNRDVRQEAYEAM
                     YGTYEQFQHTYAKTLQSNVKVNNLKARLRKYDSARHASLAANFIPESVYDTLVAAVNK
                     HLPLLHRYINLRKKLLGIDDLKMYDMYTPLSDFSTKISYEDALKKCQETLEIFGEDYS
                     AIVKEAFENRWIDVHVNEGKRSGAYSGGAYDTNAFMLLNWQDNLDNMYTLIHETGHSL
                     HSMLTRKTQPYVYGHYSLFLAEIASTTNENLLTEKLLAEVEDDKERFAILNHYLDGFR
                     GTVFRQTQFAEFEQAIYKADQEGQVLTADFLNELYGQTNEKYYGLSAEENPEIQFEWA
                     RIPHFYYNFYVYQYATGFAAASALANKIVNGSPEDKENYLNYLKAGSSDYSLNVIAKA
                     GVDMTKEDYLNDAFKVFEARLEELEALVEKGVHL"
     gene            complement(2142..3083)
                     /locus_tag="AC1PNG_001642"
     CDS             complement(2142..3083)
                     /locus_tag="AC1PNG_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HIP6702133.1"
                     /translation="MLVALDEDGQVFNVLENPAPQGRYSCPGCGGLVRYKSGKVLRSH
                     FAHVTLRDCHYFSENESAQHLSLKSCLYSWLINAEQVELEKCLPSIGQVADLFVNNSL
                     ALEVQCSSLPISRLQLRTQAYHEAGLQVLWLLGKGLWIKERLSKLHKQFLSFSMNMGF
                     HLWELDDEKKELRLRYLIHEDLRGKVHCLTKVFPFGEGNLLDILRLPFAKQALSHLTF
                     PLDRDLPRYIAQQLYYRSPNWLTLQAESYSRGENLLTKTAEEWYPHIRLPRSAIGFAQ
                     IQKDLTLVYQDFDQYYGNIEDKQKQVLYPPIIYRKPM"
     gene            complement(3147..5204)
                     /gene="metG"
                     /locus_tag="AC1PNG_001643"
     CDS             complement(3147..5204)
                     /gene="metG"
                     /locus_tag="AC1PNG_001643"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIP6702134.1"
                     /translation="MGCYQEQGFICPELKGKSMTKTPFYITTPIYYPSGKLHIGSAYT
                     TIACDVLARYKRLMGHEVFYLTGLDEHGQKIEEKAKEAGLTPQAYVDGMAVGVKELWN
                     LLDISYDKFIRTTDDYHEEVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLEE
                     VFRDENGKVTGGIAPSGHEVAWVSEESYFLRLSKYQDKLVEFFNAHPDFIQPDGRMNE
                     IMKNFIEPGLEDLAVSRTSFTWGVPVPSNPKHVVYVWIDALLNYATALGYGQEETINY
                     DKFWNGTVYHMVGKDILRFHSIYWPIMLMMLDMKLPDLLVAHGWFVMKDGKMSKSKGN
                     VVYPEMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVA
                     MINKYFSGQVPTFVANVTDFDADLAAVVADNLASYHKYMEAVDYPRALEAVWNIISRT
                     NKYIDETAPWVLAKDEADRDKLAAVMAHLTAGLRVVAHLIQPFMMTTSNAIMEQLGLG
                     TAFDLENLDFAGLPAGLTVVAKGTPIFPRLDMEEEIAYIQAQMGGSSAISQEEEKEWN
                     PADVELKNEKAAIKFEDFDKVEIRVAEVKEVAKVEGSDKLLKFRLDAGDGEDRQILSG
                     IAKFYPNEQELVGKKVQIVANLKPRKMMGLLSQGMILSAEHGDNLILLTVDPSVPNGS
                     QIG"
     gene            5304..5897
                     /locus_tag="AC1PNG_001644"
     CDS             5304..5897
                     /locus_tag="AC1PNG_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HIP6702135.1"
                     /translation="MFTKKNILTHLFFLIIAVGLKRYGVTAPDLTWNMFLALVALDFA
                     ILTNHTKSHILKVIAGLLWLFFYPNTFYMVTDIVHMHFANTVLWQRESMILFMLYVPS
                     IFFGVMAGVESVRLIFSAFSIKSYWLKLFMISGLSLLSSFAIHIGRYARLNSWDIFTR
                     PTVVIDEMLAVISWDALPFILGFTFIQIMVLVFSINE"
BASE COUNT         1643 a         1382 c         1249 g         1770 t
ORIGIN      
        1 cacttcccat agatggctca ggtctagtca aaccaccaag accgagcaag atgaaaacaa
       61 gactaatcac actgggaatc acaatcgaac gtttatagga aatcatatta gaggtgcaca
      121 cctttctcaa cgagagcttc taattcttcc aaacgagcct caaaaacctt aaaggcgtcg
      181 ttgaggtaat cttccttggt catgtctaca cctgctttag caatgacatt gagggaataa
      241 tctgagctac cagccttcaa gtagttgaga tagttttctt tgtcttctgg actgccgttg
      301 acaatcttgt ttgcaagagc tgatgcagcc gcaaaacctg tcgcatattg atagacatag
      361 aaattgtaat agaagtgtgg aatacgagcc cactcaaact ggatttctgg attttcttcg
      421 gcagaaagtc cgtagtattt ctcgtttgtc tgaccgtaca attcgttgag gaaatcagcc
      481 gtcaagactt gaccttcctg gtctgccttg tagatagctt gttcaaactc ggcaaattgt
      541 gtctggcgga agactgtgcc acggaaacca tctaggtaat ggttaagaat agcaaagcgt
      601 tccttgtcat cttctacttc tgccaagagt ttttctgtta ggagattttc attggttgtt
      661 gaagcaattt ctgccaagaa gagggaataa tgaccataga catagggttg agtcttacga
      721 gtcaacatgg aatgaagaga atgccctgtt tcatggataa gggtgtacat gttatccaaa
      781 ttatcctgcc agttgagaag catgaaggcg ttggtgtcgt aggcacctcc tgaatatgct
      841 cctgagcgtt ttccttcatt aacatggaca tcaatccagc gattttcgaa ggcttccttc
      901 acaatagccg aataatcctc accaaagatt tctaaggttt cttggcattt cttgagtgca
      961 tcttcataag aaatcttggt gctgaaatct gacaatggtg tgtacatatc gtacatcttc
     1021 aagtcatcaa tacctaggag tttcttacgc aaattgatat aacggtgcaa gagcggaagg
     1081 tgcttgttga ctgccgccac taatgtatcg taaacagatt ctggaatgaa attagctgca
     1141 agtgatgcgt ggcgagctga gtcatacttc cgtaaacgag ctttcaggtt attgactttg
     1201 acattagact gaagggtctt agcgtatgta tgctggaact gctcgtaggt accgtacata
     1261 gcttcgtatg cttcttggcg gacatcacgg tttttagact ccataaagtg gatgtagttt
     1321 ccgtgtgaaa gtggcaccaa gtgaccgtct tcatctgcga tttctgggaa acgcaggcta
     1381 gcattgtcca agactgagaa ggtttcagca ggtgattcaa aaatctcgct ggttgccgca
     1441 aggacttctt ccacctcttg tgacaggaca tggtccttgg ctgccagaag tttttcaaat
     1501 tggtggctgt actgaacaag agtaggctct tctgccttaa aggcctccag ctgctcttca
     1561 gtaatggcca taaattcagg ctcatagaag gcaaaagcct gtgaaaaggc agcatacaag
     1621 cccatggcct tagcttgaaa ttcctgatat tttccttcgc gagtatcttg gtcgttcttc
     1681 atagacgcat agacatagag tttttcaatg cgacgcataa gactaagttg cgtttcgcta
     1741 atttctaaga gacttttagc tgagtccagt aaatgacctg caaattcttt tgttttttca
     1801 gtttcagctt gtaaatccgc aagttctgct tcccaggcat catcggttgg gaaaatagta
     1861 gtcaaatccc actggtattt ttcttcaatt tcgtaacgtt gtttagacat aattaatcct
     1921 ccgtattctt tattctaaca taaaaaatcc cctctttcca acggaaagtg aggacttata
     1981 ggttactatt tcaactggaa aatagttttt ttggtattcg ctatcgcaag tcgtcactag
     2041 gctaacaaag tctacaacct tcaccgacag tagtatgact aacataaaat attgagcata
     2101 ttttcaaaag taatatatac acctccatca gctatctcta tttacattgg ttttctataa
     2161 attataggtg gatatagaac ctgtttctgc ttgtcttcaa tattgccgta atactggtca
     2221 aaatcctgat aaactaaggt taaatctttt tgtatttggg caaagccaat agcagagcgg
     2281 ggtagacgga tatgtgggta ccattcttct gccgtctttg tcagcaaatt ctcccctcgg
     2341 ctataagact ctgcctgcag ggttaaccaa ttgggggatc tgtaataaag ctgttgagca
     2401 atgtagcgag gcaaatccct atctaacggg aaagtaagat gcgataaagc ttgcttggca
     2461 aaaggcagac gcaagatgtc caataaattt ccctcgccaa atggaaaaac ctttgtcaga
     2521 cagtggacct tacctcgtaa gtcttcgtga ataaggtaac gaagacgcaa ttcttttttc
     2581 tcgtcatcca attcccagag gtgaaaaccc atgttcatac taaaagagag aaattgcttg
     2641 tgtaatttac ttaatctttc ttttatccat aggccttttc ccaacagcca gagaacctga
     2701 agaccagctt catgataggc ttgcgtcctc agctgcaaac gagaaatggg cagactggaa
     2761 cattggactt ccaaagctag actattatta acaaataaat cagcgacctg ccctatacta
     2821 ggcaggcatt tttcaagttc aacttgttcg gcattgatta accaactgta cagacaagac
     2881 ttaagtgaca gatgctgggc tgactcattc tcggaaaaat agtggcagtc ccgcagggtt
     2941 acatgggcaa aatgcgaacg cagaactttt ccggatttgt atcggaccag ccctccacaa
     3001 cctggacaag aatagcgacc ttgcggcgca ggattttcca agacattaaa aacttgtcca
     3061 tcttcatcaa gtgcaactaa cataaaacct cctctcttta ttagttcgca aaaaaacacc
     3121 cagctatgct gagtgtttga attttcttac ccaatctgac tgccatttgg cacagatgga
     3181 tcgactgtta agagtattaa attgtcacca tgttcggctg agaggatcat gccctgactg
     3241 agcaagccca tcatcttacg tggcttgagg ttggcaacga tttggacttt cttgccgacc
     3301 aactcctgct cgttcggata gaacttggcg ataccagaca ggatttggcg gtcttcgcca
     3361 tcacctgcgt ccaggcggaa tttgagcagc ttgtcagaac cctccacttt ggctacttcc
     3421 ttgacttcgg ccacacggat ttctaccttg tcgaagtcct cgaacttgat cgcagctttt
     3481 tcgtttttga gttccacgtc agctgggttc cactcttttt cttcctcttg ggaaatagca
     3541 gagctgccac ccatttgagc ttggatatag gcgatttcct cttccatatc cagacgtggg
     3601 aagataggcg ttccttttgc gaccactgtc aaacctgctg gaagacctgc aaagtcaaga
     3661 ttttcaaggt caaatgctgt tcccaaacca agttgttcca taatagcatt agacgtcgtc
     3721 atcataaatg gttgaatcaa atgtgccacc acacgaagac ctgctgtcaa gtgagccatc
     3781 accgctgcca acttgtcacg gtccgcttcg tccttagcca agacccaagg tgcagtttca
     3841 tcaatgtact tgttggtacg ggagatgata ttccaaacag cttccagagc gcgtggatag
     3901 tcaacagctt ccatatactt gtggtagctt gccaagttgt cagctactac tgccgccaag
     3961 tccgcatcaa aatcagtcac gttagcaaca aaggttggta cctgaccgct gaaatactta
     4021 ttaatcatag caaccgttcg gttgagcaag tttccaaggt cattggctag ttcgtagttg
     4081 atacggccaa cgtagtcttc tggcgtgaag gttccgtcag agccaactgg cagactacgc
     4141 atgaggtaat agcggagcgg atccaaacca aaacgctcga ccagcatttc tggatagacc
     4201 acattgccct tagacttgga catcttgcca tctttcatga caaaccagcc atgagcaacc
     4261 aagaggtctg gcaacttcat atccaacatc atgagcatga ttggccagta aatggagtgg
     4321 aaacgaagaa tgtccttgcc aaccatgtgg taaactgttc cattccagaa cttgtcgtaa
     4381 ttaattgttt cttcctgacc ataacccaag gcagttgcgt agttgagcag ggcatcaatc
     4441 cagacataga caacgtgttt aggattggac ggaacaggca cgccccaagt aaatgaggta
     4501 cgggacactg ccaaatcttc caagcctggc tcgatgaagt ttttcatgat ttcattcata
     4561 cgaccatctg gctggataaa gtctgggtga gcattgaaga actcgaccaa cttatcctga
     4621 tacttgctga ggcgaaggaa gtaggattct tctgaaaccc aggcaacctc gtggccgctc
     4681 ggagcgatac cgcctgttac cttgccgttt tcatctcgga agacctcttc taattggctc
     4741 tcggtgaaga attcctcgtc cgatacggag taccaaccag aatactctcc caagtaaatg
     4801 tcgtcctgcg ccagcaattt ttcaaaaaca gccgccacca cttcctcgtg gtaatcgtcg
     4861 gttgtgcgga tgaacttgtc gtacgagatg tcgagcaagt tccagagctc tttaacgcca
     4921 accgccatgc cgtcaacgta ggcttgaggt gtcaaaccag cttcttttgc cttctcctca
     4981 atcttctgcc catgctcatc aagccctgtc agatagaaga cctcgtggcc catgagacgc
     5041 ttgtagcgag ccaagacatc acaggcaatg gtcgtgtagg ctgaaccgat gtgaagtttt
     5101 ccagacggat aataaatcgg tgtggtgata taaaacggtg tttttgtcat actttttcct
     5161 ttcaactcag ggcaaatgaa accctgttct tgataacacc ccattttatc atattttcag
     5221 ctaaatcaca aaatggttct aactacaatt cttcctctat ctatgctata ctatgataca
     5281 actcaaacag aaagaggtca tctatgttta caaagaaaaa tattcttact catctctttt
     5341 tcctcatcat agcagtcgga ctaaaacggt acggtgtcac agcacctgac ttgacatgga
     5401 atatgttctt ggcattggta gcacttgatt ttgcaatcct taccaatcat acaaagtccc
     5461 atatcctcaa agtgatagca ggtctcctct ggctcttttt ctaccccaat actttttaca
     5521 tggtgacaga catcgtccat atgcactttg ccaacacagt actatggcaa cgagaaagca
     5581 tgattctatt tatgctttat gttccaagta ttttctttgg ggtcatggct ggcgttgaaa
     5641 gtgtacgact tattttctct gctttttcta tcaaatccta ctggctaaaa ctgtttatga
     5701 ttagtggtct ttccctacta tccagctttg ccattcatat cggacgatat gcaagactga
     5761 attcatggga catctttacc agaccaactg tcgttattga tgaaatgctt gcagttattt
     5821 cgtgggatgc tctccccttt atcctcggct tcacctttat ccaaattatg gttcttgtct
     5881 tttcaataaa tgaataagat ttttatctca tagccattat cttcaagcat cttcctgcac
     5941 tcaaggacta tcgttccttt tgggaagatt tttcaaggac ataaacaaaa ttttttgata
     6001 aatttatatc ttctccgttt ctctccatta catttttatc caaa
//
LOCUS       DBNFEG010000131         6038 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.131, whole genome shotgun sequence.
ACCESSION   DBNFEG010000131 DBNFEG010000000
VERSION     DBNFEG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.131"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            250..783
                     /locus_tag="AC1PNG_001645"
     CDS             250..783
                     /locus_tag="AC1PNG_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702136.1"
                     /translation="MKKKWLLIFSALLTVTSLVACSNSKEETTSKSSSTEQTSTTKSS
                     TKESTSESSTVTLKESTVTYLADAEIDAIQTIGEYKTSLNSLMDSYVADFDGLIVQLP
                     EEAQTTLKPYREQIVTMLEQQKSALDSQFATLGDDSTVIPEEARATVLDSLKTVRDQL
                     KQTMTVVHEQAQTLLNE"
     gene            940..1638
                     /gene="dapD"
                     /locus_tag="AC1PNG_001646"
     CDS             940..1638
                     /gene="dapD"
                     /locus_tag="AC1PNG_001646"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HIP6702137.1"
                     /translation="MTAQKMTAQEIIAFIGNAVKKTTVKVTFEGELAGPVPAEVTKLG
                     NVLFGDWKDVEPLLTNLTENVDYVVEQDGRNSAVPLLDKRNINARIEPGAIIRDQVTI
                     GDNAVIMMGAVINIGAEIGPGTMIDMGAILGGRATVGKNSHIGAGAVLAGVIEPASAE
                     PVRVGDNVLVGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEEALRTL"
     gene            1648..2775
                     /locus_tag="AC1PNG_001647"
     CDS             1648..2775
                     /locus_tag="AC1PNG_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HIP6702138.1"
                     /translation="MLDLIATRRALHQIPELGMEEFKTHAFLMETIENLLEDCSFAQV
                     RTWKTGILVYLTGSAPEKTIGWRADIDGLPIVEETELGFKSLHPDRMHACGHDFHMTI
                     ALGLLEKMAEQQPRNNLLFLFQPAEENLAGGMLMYEAGAFGDWLPDEFYGLHVRPDLK
                     VGQMATNRATLFAGTCEVKIRFTGKGGHAAFPHTANDALVAASYFVTQVQSVVSRNVD
                     PIEGAVVTFGSMHAGTTNNVIAETAFLHGTIRALTQNMSLLVQKRVREVAEGIALSFG
                     VDLEIELNPSGYLPVENNPQLADELMTYFDGVDGVEMIDCPPAMTGEDFGYLLNKVPG
                     VMFWLGVDTPYPLHNPRLSPKEEVLPFAVDKLSDFLKMKAN"
     gene            2817..3359
                     /locus_tag="AC1PNG_001648"
     CDS             2817..3359
                     /locus_tag="AC1PNG_001648"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIP6702139.1"
                     /translation="MDKKEIRQEVLQRLKALTSSQRARWSQQLTERLLSSDTYKCSKS
                     LGTYLSMPHEFDTSYLIEQAQKDGKQIFIPKTYSQGRMDFVEYNPDDLMKSRFGVWEP
                     GTYSQPVDKSVVKLIHVPGLAWNQAGFRVGYGGGFYDRYLADYQGETVSTLADFQVYD
                     FEPDVFDIPVKELLIFEELF"
     gene            3343..4020
                     /locus_tag="AC1PNG_001649"
     CDS             3343..4020
                     /locus_tag="AC1PNG_001649"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIP6702140.1"
                     /translation="MKNFFDKRYPVTNALLAVTALVFLLIQIFRFGQTTTAYTIFEFG
                     GMYGQVVRYDPTQLWRLISPIFVHIGWEHFIFNSITLLGLGYQLEGLFGSRRFFLLYL
                     LSGIMGNLFVLFFTPDVVGAGASTSLFGLFAAMALLRKFSRSPYLQVLGQRYMVLLGL
                     NLVLGLFNPTISMAGHIGGAIGGCLVVIFLPPLVEKDLFTGKQIFFSFIGYMSLIVFL
                     LGIFYLI"
     gene            complement(4039..4938)
                     /gene="galU"
                     /locus_tag="AC1PNG_001650"
     CDS             complement(4039..4938)
                     /gene="galU"
                     /locus_tag="AC1PNG_001650"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIP6702141.1"
                     /translation="MKKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALRSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKNDLLKLVDETTGIRLHFIR
                     QSHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDTKAVPLTKQLMNDYEKTHASTI
                     AVMPVPHEEVSAYGVIAPQGEGVNGLYSVETFVEKPKPEDAPSDLAIIGRYLLTPEIF
                     EILENQKPGAGNEIQLTDAIDTLNKTQRVFAREFKGNRYDVGDKFGFMKTSIDYALQH
                     PQVKDDLKQYLIDLGKKLDTKKN"
     gene            complement(4984..6000)
                     /locus_tag="AC1PNG_001651"
     CDS             complement(4984..6000)
                     /locus_tag="AC1PNG_001651"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIP6702142.1"
                     /translation="MTKQTIAILGPGSWGTALGQVLNDNGHTVRIWGNVPEQIDEINK
                     EHTNKRYFKDVILDENIKGCKDLTEALDGVDAILFVVPTKVTRLVAKQVAQALKHKVV
                     VMHASKGLEPDSHKRLSEVLEEEIPAELRSEIVVVSGPSHAEETIVRDLTLISAASKD
                     LETASYVQNLFSNHYFRLYTNNDVIGVETAGALKNIIAVGAGALHGLGYGDNAKAAII
                     ARGLTEITRLGVAMGANPLTYSGLSGVGDLIVTGTSVHSRNWRAGDQLGRGEKLEDVE
                     RNMGMVIEGISTTKAAYELAQELDVYMPITQAIYKVIYQGAGIEDAIKEIMTGEFRHE
                     NEWH"
BASE COUNT         1601 a         1240 c         1401 g         1796 t
ORIGIN      
        1 atgaatatgc caatttgttc ggtataaaat ttctagtcaa tccggcagat tttatcaagc
       61 tttatccgag tgtttagtca ggaacggtat gaaataccct ataaaaacct agtgattctc
      121 ataatgattc tgcatttgag agggctactg gttgtatatt ctaaaaagat aaattgtttt
      181 ctagatatac ttgactttca tacttaaaaa taatattatc tagtagtaat aaacaaaagg
      241 agttttataa tgaaaaagaa atggttgctg attttctcag ctcttctaac agttactagt
      301 ttggtggctt gctctaatag taaggaagaa acaacgtcaa aaagctcttc aacggaacaa
      361 acatccacaa cgaaatcctc tactaaagag tctacatctg aatcctcgac tgttacttta
      421 aaagagtcaa cagtgactta tctggcagat gcagaaattg atgcgattca gactatcgga
      481 gaatataaaa catcattgaa ctcattgatg gactcttatg ttgctgactt tgatgggtta
      541 attgttcagt taccagaaga agcacagact actttaaaac catatcgtga gcaaattgta
      601 acgatgcttg aacaacaaaa atcagcacta gattcacaat ttgcgacatt aggggatgac
      661 tcgacagtga ttccagaaga agcacgagct actgttttgg atagtttaaa aacagttcgt
      721 gatcaactaa aacaaacgat gacagttgtg catgaacagg cacaaacatt attaaatgaa
      781 taacagagaa agatttggct cctgcccaat gttattaagt tagctaactt aataacattg
      841 ggaactgagg gcctcttttc catttttttg catttcttcc caaattttca gaaaattttg
      901 atataataga aaaaatatta tttttagatt ggaatcatta tgactgcaca aaaaatgact
      961 gcacaagaaa tcattgcctt tatcggtaat gcggtgaaaa agacaactgt taaagtgact
     1021 tttgaaggtg aattggctgg tcctgttcct gcagaagtga caaaacttgg caacgtcctc
     1081 tttggtgact ggaaagacgt cgagccactt ttgacaaact tgactgaaaa tgtagactac
     1141 gtggttgagc aagacggccg caattcggct gtgcctttgc tagacaaacg taacatcaat
     1201 gcccgtatcg agccaggtgc cattatccgt gaccaagtga caatcggtga taatgccgtt
     1261 atcatgatgg gtgcggtcat taatatcggt gcggaaatcg gcccaggtac catgattgac
     1321 atgggtgcta tccttggtgg tcgtgcgacg gttggtaaaa acagccacat cggtgcaggt
     1381 gcggtccttg caggtgttat cgagccagct tcggctgagc cagtgcgtgt tggcgacaat
     1441 gttttggtcg gtgccaatgc cgttgtcatc gagggagtac aaatcggtag tggctctgtt
     1501 gtcgcagccg gtgctattgt gacccaagac gtccctgaaa acgtagtggt ggcaggtgtg
     1561 ccagcccgca tcatcaagga aatcgacgct caaacccaac aaaaaacagc cttggaagag
     1621 gctttgcgga cactctaaga ggtagctatg ctagatttaa tcgcgacacg ccgggccctc
     1681 caccagattc cagagctggg tatggaggag ttcaagacac acgcctttct tatggaaact
     1741 attgagaact tgttggaaga ttgctctttt gcccaagtcc gtacatggaa gacaggtatc
     1801 ctggtctatc tgacaggttc tgctcctgaa aagaccatcg gctggcgggc agatattgac
     1861 ggcctgccta tcgtggaaga aacggagctg ggcttcaagt ccctccaccc agaccggatg
     1921 cacgcctgcg gacatgattt ccacatgacc attgccctgg ggcttttgga gaaaatggca
     1981 gagcagcaac cgagaaataa tcttctcttc ttgtttcagc ctgcggagga aaatctagca
     2041 ggtggtatgc ttatgtatga agcgggggct ttcggggatt ggttgccaga tgaattttat
     2101 gggctccatg tccgacctga cctcaaagtc ggtcaaatgg ccaccaaccg tgcgaccctc
     2161 tttgctggaa cctgtgaagt caagatacgg tttacaggaa agggtggcca cgcagcattt
     2221 ccccataccg ccaatgacgc cttggtggca gccagctact ttgtgactca ggtgcagtcg
     2281 gtggtcagcc gtaacgttga cccgatcgag ggagcggtgg tgaccttcgg ctccatgcat
     2341 gctggcacga ccaacaatgt cattgctgaa acggcctttt tgcacgggac cattcgggcc
     2401 ttgactcaga acatgagcct cttggtgcaa aaacgggtgc gggaagtggc cgagggaatc
     2461 gccctgtcct ttggagtgga cttggagatc gagcttaatc ctagcggtta tctgcctgtg
     2521 gagaacaatc cccagctggc tgatgaactg atgacctact ttgatggtgt tgacggagtg
     2581 gagatgattg attgtccacc tgccatgacg ggagaggact tcggctacct gcttaacaag
     2641 gttccgggtg ttatgttctg gttgggtgtg gatacgcctt atccccttca caatccacgt
     2701 ctcagtccca aagaggaagt gcttcccttt gctgtggata agttgagcga ttttttgaaa
     2761 atgaaagcaa actagactgg agtttccagt ctttttctga aaagaaagga gttcctgtgg
     2821 ataaaaaaga aattcgacaa gaggtcttgc agagattgaa agccctgact agtagccagc
     2881 gagcaagatg gagtcagcaa ctaacagaaa ggctcctttc atctgatacc tataagtgct
     2941 caaagtcgct tggaacctac ctttccatgc cccatgagtt tgacacttct tacctgattg
     3001 agcaggctca aaaggatggc aagcagattt tcatccctaa gacctattca caaggtcgta
     3061 tggattttgt agaatataat cctgatgatt tgatgaaatc taggtttgga gtttgggaac
     3121 ctgggactta ttcacaacct gtggataagt ctgtggttaa gttgatccat gttcctggtt
     3181 tagcttggaa tcaagcgggt tttcgagtgg gttatggagg cggtttttat gatcgctacc
     3241 tagcagatta tcaaggggag acggtatcga ccttggcaga ctttcaagtc tatgattttg
     3301 aaccagatgt atttgatatt ccagtaaagg agttgttgat ttttgaagaa cttttttgat
     3361 aagcgctatc ctgtgaccaa tgccttgttg gcagtcacgg ctcttgtttt tcttttgata
     3421 caaatatttc gatttgggca gaccactaca gcctatacga tttttgaatt cggtggtatg
     3481 tacggtcaag tggtgcggta tgatccgact cagttgtggc gcttgatttc tcctatcttt
     3541 gtccacattg gttgggaaca ttttatcttt aacagcatta ccttgcttgg tttgggttat
     3601 caattagaag gactgtttgg gtcacggcgc ttctttctcc tctatctctt gtctgggata
     3661 atgggcaatc tttttgttct cttctttaca ccagatgtag ttggtgcagg tgcatcgacc
     3721 tctctgtttg gtctttttgc agctatggca cttttgagga aatttagtcg cagcccttat
     3781 ctacaggttc tcggtcaacg ttatatggtt cttttgggat tgaacttggt cttgggactt
     3841 tttaatccaa ccattagcat ggcaggacat attggaggag ctattggtgg ttgtttggtc
     3901 gtgattttcc ttccccctct tgtggaaaag gatctcttta caggaaaaca gatttttttc
     3961 agcttcattg gctatatgag cttgatcgtt tttctactag ggatattcta tttgatataa
     4021 gaaaaaaact agcgattgct agtttttctt tgtatctaat tttttaccaa gatcgattaa
     4081 gtattgtttc aaatcatctt ttacttgagg gtgttggagg gcgtagtcga tagaagtttt
     4141 catgaaaccg aacttatccc caacatcgta acggttgcct ttgaattcac gagcgaaaac
     4201 acgttgcgtt ttgttcagag tatcaatggc atctgtcaat tgaatctcgt tcccggcacc
     4261 tggtttttgg ttttctaaaa tctcaaagat ttcaggagtg agtaagtagc gtccgataat
     4321 agcaaggtca gaaggagcgt cttctggttt tggtttttcc acaaaggttt ccacactata
     4381 gagaccgttg acaccttcac cttgtggagc gataacaccg tatgcagaaa cttcttcatg
     4441 aggaacaggc ataacagcaa tggttgaagc atgtgttttt tcatagtcgt tcatgagttg
     4501 cttggtcaat gggacagctt ttgtatctgt gatgtccatc aagtcatcgc cgagcatgac
     4561 aacaaatggc tcatttccga caaaggcttt tgcttgaaga acagcatccc caagtccacg
     4621 tggatggctt tgacgaatga agtgtagtcg aatacctgtt gtctcatcaa ccagtttcaa
     4681 caaatcattt ttgccttttt ctttgaggtt gtattccaat tcaaagtttg aatcaaagtg
     4741 gtcttcgata gaacgttttg acttaccagt gaccaccaaa atatcttcaa tacctgaacg
     4801 gagggcttct tcaacgataa attggatggt tggcttgtca acgattggca acatttcctt
     4861 ggcaagagcc ttagtagcag gcaagaaacg tgttcccaaa cctgcagctg ggataacagc
     4921 ttttctaact tttttcataa gtatgtaatc ctttataatt tatttttttg tgtttaataa
     4981 gaatcaatgc cattcatttt catggcggaa ttcacccgtc atgatttctt tgatggcatc
     5041 ttcaatacct gcaccttggt agatgacctt gtagatggct tgggtaatag gcatgtaaac
     5101 atctaattct tgagccaact cataggctgc cttagttgtt gaaatacctt caatcaccat
     5161 tcccatattg cgttctacat cttcgagttt ttcaccgcga ccgagctgat ctccagctct
     5221 ccagttgcgg gagtggacag atgtaccggt aacaatcaaa tcaccaacac cagaaagacc
     5281 gctgtaagtc agaggatttg cgcccatagc aacgcctaga cgtgtgattt ctgtcaaacc
     5341 gcgagcgata atagctgcct tggcattatc accataacca agtccgtgta aagcgccagc
     5401 gccgacagca ataatatttt taagggcacc tgctgtttct acaccgatga catcattatt
     5461 tgtataaagt cggaagtagt ggttgctgaa aagattttgg acgtagctgg ctgtttccaa
     5521 gtcttttgaa gctgcagaaa tcaaggttaa atcacggacg atagtctctt ctgcatgact
     5581 tggccctgaa accacgacga tttcagaacg gagttcagca ggaatttctt cctccagtac
     5641 ctcagacaag cgcttgtgac tatcaggctc taagcctttt gaagcgtgca tgacaactac
     5701 cttgtgtttg agagcttgtg cgacttgctt ggcaacgaga cgtgttacct tggttggtac
     5761 aacgaaaaga atagcgtcaa caccgtccaa ggcttcagtc aaatccttac agcccttgat
     5821 gttttcatct aggataacat ccttgaaata gcgcttattt gtatgttctt tattgatttc
     5881 gtcgatttgt tcggggacat ttccccaaat acggacagta tgaccgttgt cgttgagaac
     5941 ttgtcctagg gcggtgcccc atgaaccggg tcccaagatg gcaatggttt gtttggtcat
     6001 aaaaaatcct ttctagttgg tgtaatttag atatatta
//
LOCUS       DBNFEG010000132         6024 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.132, whole genome shotgun sequence.
ACCESSION   DBNFEG010000132 DBNFEG010000000
VERSION     DBNFEG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.132"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            20..1810
                     /locus_tag="AC1PNG_001652"
     CDS             20..1810
                     /locus_tag="AC1PNG_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIP6702143.1"
                     /translation="MKKTTKLFALAGVTLLSASVLAACGSKQSGAAKQELSFPAEVKQ
                     DGTAVSDAQLKYAWVSPTTSSGLLIDELTENTTDSTFGGFVDISMFGYDGERKLDDSG
                     LAKAEFDVKGKKITISLTGKDYKWSDGEPFTINDYIFTIKSMASKDYTGIRFDDKFLN
                     IEGMQEFVDGKASDISGIKKVDDYTVELTVKEMSPSMMYAGGDVPAYIQPEHIYKDIP
                     VADWEKSEYSRTAKLVGMGPWKIKEIVNGESITYVPNEYFFKGTKPKTSSLKIDIVSP
                     DTIVSEMKAGNYDIAEMPVDQLDSYKDVSNLNIVGQLESSYEYISFNLGKYDEAANKN
                     VMDEKAKMNDVKLRQAIAYAIDTKTAGKSLYNGLYHPAKSLIISFFGDIHDSELEGYS
                     YNPEKAKKLLDEAGYKDVDGDGIREGKDGKAFKITFAARKRTEANEALVQQYIAWWKE
                     VGLNVELYTGRTVEGKSFYNSVQANDPAIDMYAGGWSTGYDPNPSGLWGSVAAFNMSR
                     FVSDENTKLLEAISSAESFDDKKNVENYKAWQKYAHEQAFAIPTFESESIVALNKRVK
                     NYDTNYGSASGKGIALENIELTADKGVVAE"
     gene            2187..3185
                     /locus_tag="AC1PNG_001653"
     CDS             2187..3185
                     /locus_tag="AC1PNG_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6702144.1"
                     /translation="MATEKPLLDIKDLHVGFRIGDDYFDAVDGVDISLQKNEILAIVG
                     ESGCGKSTLATTIMGLHNPLNTKITGSIQYNDMELIGMNEAKYNTVRGNDIGMIFQDP
                     LASLNPLMTIGAQIDEALFYHTDLDANARTERVLELLAQVGIPNPKRTFKQYPHELSG
                     GMRQRIIIAMALSCKPPIIIADEPTTALDVTIQAQILDLLNDIQAETGSGIILITHDL
                     GVVAETADRVAVMYGGQFVEVAPVEELFTNPKHPYTRSLLKSNPQSGSEGGDLHVIEG
                     IVPPITKMVRKGCRFAPRIPWIGAEAHEENPSMHEVGPNHFVRCTCHETFYFEGEA"
     gene            3188..4129
                     /locus_tag="AC1PNG_001654"
     CDS             3188..4129
                     /locus_tag="AC1PNG_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP6702145.1"
                     /translation="MGFIEVKDLKVHYPIRSGFFNRVTDHVYAVDGVNIEFEEGKTYG
                     LVGESGSGKSTIGKAIIGLERATSGQIIYEGQNVTNKSRGKKGNFNRDVQMIFQDSLS
                     SFNPKKRILDIIAEPIRNFDRLSPDEEKKKVLQLLDTIGLNEEALIKYPHEFSGGQRQ
                     RIGVARALASNPRLIIADEPVSALDLSVQAQVLNYMKRIQDEFKLSYLFISHDLGVVQ
                     HMCDELFIMYRGRFVETGNKNDIYNNPQHIYTKRLLSAIPTIDPLNRLKNKEKRLAAE
                     KEYQEMQGQYYDENGRVYDLRSFSETHQVALPEGGQN"
     gene            4131..5087
                     /gene="opp4B"
                     /locus_tag="AC1PNG_001655"
     CDS             4131..5087
                     /gene="opp4B"
                     /locus_tag="AC1PNG_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HIP6702146.1"
                     /translation="MWKTVLRRLLMMIPQIIILSVIAFFVAKMMPGDPFTGLIDPNID
                     PAIIEQKRIAAGYYDPIPIQYFRWVGNLLQGDFGQSVIFKQPVVDVIMQRLNNTIGLS
                     LLTMVLTYLIALPLGMIAGRYQNSLADKIIGVYNFLTFSTPTFVFAILMLWMFGFSLG
                     WFPTRGSISGGVEGFAATLSRLHHMILPAITMAILSTTVTIQYLRTGIIDAKSQDYVR
                     TARAKGVPERVVYNRHIFRNSILPIASFLGYELTGLIGGSIFIENIFTYPGIGQLFFN
                     SISSRDYSVILALLLVFGMGTLLGTLISDIIMSIVDPRIRVK"
     gene            5101..6003
                     /locus_tag="AC1PNG_001656"
     CDS             5101..6003
                     /locus_tag="AC1PNG_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6702147.1"
                     /translation="MSKENKKQVQSASTPPGGFRVIAREFMKDRLALTSLIILVTVLL
                     AVFIGALLLNQEQVMKVNLLGRYLEPGVDGYILGTDEGGKDIFGQLIIGARNSIIIGF
                     TITIITSIVGISVGLISGYYGGRLDGFLMRIVDFIMILPVTMLIIVFVTVIKNYTIYH
                     FILIMSAFYWTAKARLFRTRTLSEASLDYVNASKTLGTSDFMIMFREILPNLSSLIIV
                     NLTLNFAGNIGIETSLTYLGFGLPSTVPSLGTLIANARNADILENKTWIWLPAAIFIL
                     VMMLCINYVGQAFQRAADAKQRLG"
BASE COUNT         1765 a         1065 c         1338 g         1856 t
ORIGIN      
        1 aagaaaacag aggtgtttta tgaaaaagac aacgaaactt tttgcacttg caggtgtgac
       61 tctcctttca gcatctgttc ttgcagcatg tggttcaaaa caatcaggtg cagcaaaaca
      121 ggaattgtca ttcccagctg aagtaaaaca agacggaaca gcagtgtctg acgctcagtt
      181 gaaatacgca tgggtatcac caacaacttc atcaggtctc ttgattgatg agttgactga
      241 aaatacgact gactcaacat ttggtggttt tgttgatatt tcaatgttcg gttacgacgg
      301 agaacgtaag ttggacgact caggtcttgc caaggctgaa tttgatgtga aaggtaaaaa
      361 aatcacaatt agcttgacag gcaaggatta caagtggtca gatggtgagc cattcacaat
      421 caacgactac atctttacaa ttaagtcaat ggcaagcaaa gactatacag gtattcgttt
      481 tgatgataaa ttcttgaaca ttgaaggcat gcaagaattt gttgatggaa aagcatcaga
      541 catctctggt attaagaaag ttgatgacta tacagtagaa ttgactgtaa aagaaatgtc
      601 accttcaatg atgtatgcag gtggtgatgt cccagcctat atccaaccag aacacatcta
      661 taaagacatc cctgttgcag actgggaaaa gagcgaatac tcacgtactg cgaagcttgt
      721 tggtatggga ccgtggaaga ttaaagaaat tgttaatggt gagtctatca cttacgtacc
      781 aaacgaatac tttttcaagg gtacgaaacc aaaaacaagc tcattgaaga ttgacatcgt
      841 atctcctgat actattgttt ctgaaatgaa agcggggaat tatgatattg ctgaaatgcc
      901 ggttgatcag ttagattcat ataaggatgt ttctaacttg aatatcgttg gtcagttaga
      961 gtcgtcttat gagtatatct cgtttaatct tggtaaatat gacgaagcag ctaataagaa
     1021 cgttatggat gaaaaggcta agatgaatga tgtgaaactt cgtcaagcaa ttgcctatgc
     1081 aattgatacc aagacagctg gcaaatcatt gtacaacggt ctttaccacc ctgcaaaatc
     1141 attgattatc tcattcttcg gggatattca tgattcagag ttggaaggct attcatacaa
     1201 tcctgaaaaa gcgaaaaagc ttttggatga agctggctac aaagatgtag atggcgatgg
     1261 tatccgtgaa ggtaaagacg gtaaggcgtt caaaattacc tttgctgctc gtaaacgtac
     1321 tgaagctaac gaagcgcttg tacaacaata tattgcttgg tggaaagaag ttggcttgaa
     1381 tgtcgaactt tacacaggac gtacagttga aggtaagtcg ttctacaact cagttcaagc
     1441 taatgatcct gctattgata tgtatgctgg tggatggtca acaggttacg atccaaatcc
     1501 aagtggtcta tgggggtctg tcgcagcctt caatatgtca cgtttcgtgt ccgatgaaaa
     1561 caccaagtta ttggaagcta tcagctcagc agagtcgttt gatgataaga aaaacgttga
     1621 gaactataag gcttggcaaa aatatgctca cgagcaagca tttgctattc caacatttga
     1681 atctgagtca attgtagcac ttaacaaacg tgtgaaaaac tatgacacta actacggttc
     1741 agcttcaggt aaaggtatcg ctcttgagaa tatcgaactt actgctgata aaggtgtagt
     1801 ggcagaataa tttgttagaa gacaatagaa tctgaaaaag ccaatttgat tggctttttt
     1861 cagattgcta ttggaatttt cagataattg tggtatagta gactcagtac acaaaaatga
     1921 gtccctacta acaaaaactc aagatgaaga acattaacct actcaattcc taattgagac
     1981 agtaataggg atggtcttga tttatgattg gatataggta cattttttac ttctgggtaa
     2041 tcgctatcaa acgataattt gatagaaata tgttattcta gagtagttat ataaagtcag
     2101 ttaatcaaac gttatatgaa cttgataatc gtaaggctat tgaattgatt gagcttgatt
     2161 agctggcaca taaaggagga aaaagcgtgg ctaccgaaaa accgctttta gacattaaag
     2221 atttacacgt cggtttccgt attggagatg attattttga cgctgttgac ggtgttgaca
     2281 tctcattgca aaaaaatgaa attttggcaa ttgtagggga atctggctgt ggtaaatcaa
     2341 ctttggcaac gaccatcatg ggcttgcata accctttgaa taccaaaatc acaggaagca
     2401 tccagtacaa tgatatggaa ttgattggca tgaatgaagc taagtacaat acagtacgcg
     2461 gtaatgatat tggtatgatt ttccaagatc ccttggcttc tctaaatcca ttgatgacga
     2521 ttggtgcaca gattgatgag gcgctgttct atcacactga tttggatgcc aatgctcgta
     2581 cagagcgagt tttggaactc ttggctcagg taggtattcc aaatcctaag cggacattta
     2641 aacagtatcc acatgagctt tcaggtggta tgcgtcagcg tatcattatt gcgatggcct
     2701 tgtcttgtaa accgccaatt attattgcgg acgaacctac aacagccttg gatgttacaa
     2761 ttcaagcaca aatcttggat ctcttaaatg atattcaagc ggagacaggt tctggtatta
     2821 ttttgattac ccatgacttg ggagtagttg cagaaactgc tgatcgtgtg gctgtcatgt
     2881 acggtggtca gtttgtcgaa gtagcgccag tagaagaact ctttaccaat ccaaaacatc
     2941 catatacacg ttcgctcttg aagtcaaatc ctcaatcagg tagcgaagga ggcgatcttc
     3001 atgttattga aggtattgtg ccgccgatta caaaaatggt tcgtaaaggt tgccgttttg
     3061 ctccgcgtat tccgtggatt ggagcagagg ctcatgaaga gaatccgagc atgcacgagg
     3121 taggtccaaa tcactttgtt cgttgtacct gccatgagac attctatttt gaaggggagg
     3181 cctaatagtg ggatttattg aagtaaaaga tttaaaagtc cattatccaa ttcgtagtgg
     3241 cttctttaac cgtgtgacgg accatgtcta tgctgtggat ggtgttaata ttgagtttga
     3301 agaaggaaaa acctatggtt tggtaggtga gtcaggttct gggaaatcaa ccattggtaa
     3361 ggcaattatc ggtttagagc gtgccacttc tggtcagatt atctatgaag gacaaaatgt
     3421 aaccaacaaa tcacgtggta aaaaaggaaa ctttaatcgt gatgttcaaa tgattttcca
     3481 agattctctt tcaagtttta acccgaaaaa acgtattttg gacatcattg cagaaccaat
     3541 tcgtaacttt gaccgtttat ctccagatga agaaaagaaa aaagttcttc aactcttgga
     3601 tacaatcggt ctaaatgaag aagctctgat taagtatcct cacgaattct caggtggtca
     3661 acgccaacgt atcggtgttg ctcgtgcttt ggctagtaat cctcgtttga ttattgcaga
     3721 tgagccggtt tctgccttgg atttgtctgt gcaggcacag gtcttgaatt atatgaaacg
     3781 tatccaagat gagtttaagc tcagctatct ctttatttcc catgacctcg gtgttgtgca
     3841 acacatgtgt gatgaattgt tcatcatgta tcgcggacgt ttcgtagaaa caggaaataa
     3901 gaatgatatt tataataatc cacaacacat ttatactaaa cgtttgttgt cagctattcc
     3961 aacgattgac ccactcaatc gattgaagaa taaagaaaag cgtttggctg ctgaaaaaga
     4021 atatcaagaa atgcaaggtc aatactatga tgaaaacggt cgtgtgtacg atttgaggtc
     4081 cttctcagaa actcatcaag tagcccttcc agaaggaggt cagaactaag atgtggaaaa
     4141 cagtattacg tcgtttactc atgatgattc cgcagattat tattttgagt gtcattgcgt
     4201 tctttgttgc gaagatgatg ccgggggatc cctttacagg attgattgac cccaatattg
     4261 atccagcaat cattgaacaa aaacggattg cggcaggtta ctatgaccca atccctattc
     4321 agtatttccg ctgggtaggc aatcttttgc aaggagattt tggtcagagt gttattttta
     4381 aacaaccagt tgttgatgtc atcatgcagc gcttgaataa tacaattgga ttatccctct
     4441 tgacgatggt attgacttat ttgattgcac ttccattggg gatgattgca ggtcgttatc
     4501 aaaactcact tgcagataaa attattggcg tgtataactt cttgacattt tcaactccaa
     4561 ctttcgtttt tgccatcctc atgctctgga tgtttggctt tagtttgggc tggttcccga
     4621 cgcgcggttc gattagtggt ggtgtggaag gatttgcagc gactctcagt cgtttacacc
     4681 atatgattct gccagcgatt acaatggcta ttttgtcaac aacagttact attcaatatc
     4741 tccgtacggg gattattgat gctaagagtc aggactatgt tcgtacagct cgtgccaaag
     4801 gtgttcctga gagagttgtt tataaccgtc atatcttccg gaattctatt ttgccaattg
     4861 catctttctt gggctatgaa ttgacaggat taattggtgg ttctatcttt attgaaaata
     4921 ttttcactta cccaggtatc ggtcagttat tctttaactc tatttctagt cgtgactata
     4981 gtgttatctt agctttattg ttggtatttg gtatgggtac tttgttgggg acattgattt
     5041 cagatattat catgagtatc gtggatccac gtatccgtgt gaaatagaag gaggagtaag
     5101 gtgagtaaag aaaataaaaa acaagtacaa tctgcttcaa ctcctccagg tggcttccgt
     5161 gtcatcgcgc gggagtttat gaaagatcga cttgcattga cctcattaat tatcttagtg
     5221 acggttctct tagcagtctt tatcggtgca cttctgttga atcaagaaca agttatgaaa
     5281 gtcaaccttt tgggacgtta cttggaacca ggtgttgacg gctatatctt ggggacagat
     5341 gaaggtggta aagatatctt tggtcagctg attatcggcg ctcgtaactc aatcattatc
     5401 ggttttacca ttacgattat tacaagtatt gtcgggattt ctgtcggtct gatttctggt
     5461 tattatggcg gtcgtttgga cggattcttg atgcgtattg tcgatttcat catgattttg
     5521 ccggtgacaa tgttgattat tgttttcgtg acagttatca agaactatac gatctatcat
     5581 ttcattctca tcatgagtgc cttttattgg acggccaagg ctcgtctatt ccgtacaagg
     5641 actttgtcag aggcaagttt ggactatgta aatgcatcaa aaacattggg aacaagcgac
     5701 tttatgatta tgttccgtga aattttacca aacttaagtt cattgattat cgttaacttg
     5761 accttgaact ttgcgggaaa tatcggtatc gagacttcgt tgacttatct tggtttcggt
     5821 ctgccatcga cagtaccaag tttgggtacc ttgattgcca atgcccgcaa tgcagatatt
     5881 ttggaaaaca agacatggat ttggttgcca gcagccatct tcatccttgt gatgatgctc
     5941 tgtatcaact atgtcggaca agctttccaa cgtgcagcag atgctaagca gcgtttaggt
     6001 taattgtgaa aagggttcct ttgg
//
LOCUS       DBNFEG010000133         5998 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.133, whole genome shotgun sequence.
ACCESSION   DBNFEG010000133 DBNFEG010000000
VERSION     DBNFEG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.133"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(161..1048)
                     /locus_tag="AC1PNG_001657"
     CDS             complement(161..1048)
                     /locus_tag="AC1PNG_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIP6702148.1"
                     /translation="MNTQMYTKFDKRMLIVNKILLAFLVLITVVPMVYILVASFMDPQ
                     ALVSKGISFNPKDWTVEGYQRVFSDKSILRGFFNSLFYSFSFAFVTVAISVMTAYPLS
                     KKDLVGKKWINAFLVFTMFFGGGLVPTYLLVKDLGMLNTVWAIIIPGAVNVWNIILAR
                     TYFQGLPDELVEAAVMDGANEFQIFWKIMIPLAKPIMFVLFLYAFVGQWNSYFDAMIY
                     IKDPDLEPLQLVLRKILIQSEPAKDMIGAQAAMNEMKRIAEMIKYATIVISSLPLIVM
                     YPFFQKYFDKGIMAGSLKG"
     gene            complement(1058..1978)
                     /locus_tag="AC1PNG_001658"
     CDS             complement(1058..1978)
                     /locus_tag="AC1PNG_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6702149.1"
                     /translation="MKTSKLQQAGLVDRIKQQKLLLLMLLPGLVLTFIFRYIPMYGVL
                     IAFKDYNPLKGVLGSEWIGFEEFTKFLSSPNFGTLLANTLKLSVYGLLLGFLPPIILA
                     IMLNQLLSDKAKKRIQLVLYAPNFISVVVIVGMIFLFFSVGGPVNSILGMFGIEANFL
                     TDPDFFRPLYILSGIWQVMGWASTLYTATLVNVDPALIEAAKLDGANIFQRIWHIDLP
                     ALKPVMVIQFILAAGGIMNVGYEKAFLMQTSLNLTSSEIISTYVYKIGLVSGDYSYST
                     AVGLFNALINIILLIAVNKIVKRINDGQGL"
     gene            complement(2083..3072)
                     /locus_tag="AC1PNG_001659"
     CDS             complement(2083..3072)
                     /locus_tag="AC1PNG_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIP6702150.1"
                     /translation="MEEQKSITMKDVARVAGVSVGTVSRVINNEPGIKESTLEKVNTA
                     IRELNYIPDVYARGMKKNKTETIALIIPTVWHPFFGEFAYHVEVELSKKNYKLLLCNI
                     SGPKRELDYLTMLQQNKVDGIIAITYSPIEDYLSSNIPFVSIDRVYENKDIACVSSDN
                     RAGAELAANTLIAKGGRHFAFIGGHNKTINETKKRRLYFEKRILEAGFPCHVLDLEEP
                     YDDFVGQVEEFLINNPQVDAIFTINDFTALDTLAVLEKLGRRVPEDVQVIGYDGIQLA
                     SEKSLALSTIRQPLESMAQEAVACLIDIIEKRERPLQVTLPISYLEGKTTKKF"
     gene            complement(3229..3774)
                     /locus_tag="AC1PNG_001660"
     CDS             complement(3229..3774)
                     /locus_tag="AC1PNG_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702151.1"
                     /translation="MSLQNRVEEMYKDHEVKPYISPERDLAAWLLEAKPVPKRNMVRL
                     EEGILPGDIILLWRISLGSFETTTPYSKYFEYMYGINGPAHMEQLIADGYAYVESAFD
                     SLDHITSTAKKNILKAEGVTGLSKMKAADLDTALKDNLTEEKLAPYFTVRGYALTEKG
                     RAALENHPEVLAKHPMKKMYK"
     gene            complement(3775..4152)
                     /locus_tag="AC1PNG_001661"
     CDS             complement(3775..4152)
                     /locus_tag="AC1PNG_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HIP6702152.1"
                     /translation="MIQSIGQVMLYVNDIEASAQFWKEKMGFERVEKQVQGPQTSYII
                     APKSDSEVQFVLHDKAEVAEMEPELNLGTPSILMTSVDVAKTYEELVERGVTTNPVMD
                     LGFMKVISFADNDGNYYAIREVK"
     gene            complement(4237..4590)
                     /locus_tag="AC1PNG_001662"
     CDS             complement(4237..4590)
                     /locus_tag="AC1PNG_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HIP6702153.1"
                     /translation="MFENLFAPDGSLVWHTALFYSFSAIILATVCFHIIKNFMEWHHN
                     NRAPKESCKAKLVSKRTHVFGNEVARTNYYVTFEWEGKRQEFRLRSDEYALLAEGDVG
                     TLHFQGTRFLGFERL"
     gene            4733..5536
                     /locus_tag="AC1PNG_001663"
     CDS             4733..5536
                     /locus_tag="AC1PNG_001663"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIP6702154.1"
                     /translation="MKKILVLALASSILLTACTNNSTANSSSSTASSSSPSSTTTADT
                     TASSQYAKDLAYAINNPKATFPQLSNEIGENEAAVKIKTTEGDITIKLFPEQAPLTVE
                     NFLTHAKEGYYNGTIFHRVIKDFMIQGGDPLGNGTGGESIWSGKDTTIDAGNGFKDEI
                     SAFLYNIRSSLSMANAGAGTNGSQFFINQNTTDMSSQLSSSSYPGKIIDAYKNGGNPN
                     LDGKHTVFGQVIEGMDIVDKIASVETDNSDKPKTDVKIESIEILKDYTF"
     gene            5667..>5998
                     /locus_tag="AC1PNG_001664"
     CDS             5667..>5998
                     /locus_tag="AC1PNG_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HIP6702155.1"
                     /translation="MEQLNLITNFLRIKDKNITITDEYDMGTHLELHGYLDYMAPKCP
                     ECKGQMAKYDYQKASKIPYLETAGYPLLIRLKKRRFKCKECGKMAVAETPLVKKNHQI
                     SVAVSQKIA"
BASE COUNT         1841 a         1256 c         1173 g         1728 t
ORIGIN      
        1 atatctttca gtttcctttt attactaggc aacgagctga aggctgtact gaagtacggc
       61 atagcgagtt caaataatac atgattattt gaagctggag ataaggaaac gaagtttcct
      121 ctttcgtcga cgtaagaaaa gagaaaatga aagatattta ttaacctttt aatgaaccag
      181 ccatgatccc cttgtcaaag tatttctgga agaatggata catgacaatg agtggcaagc
      241 tagagatgac aatggtcgca tacttgatca tttctgcgat tcgtttcatt tcattcatgg
      301 cagcctgggc accaatcatg tccttagcag gctcgctttg aatgaggatt ttacgtagca
      361 caagttggag tggctccaag tctgggtcct tgatgtaaat catggcatca aagtaagaat
      421 tccattgacc gacaaaggca tataggaaaa gtacaaacat gattggctta gctaatggaa
      481 tcatgatttt ccagaaaatc tgaaattcat tggcaccgtc cataaccgct gcttctacca
      541 actcatctgg caagccttgg aaataggtcc gtgccaagat gatattccac acattgactg
      601 caccaggaat aataatggcc cagacagtat tcaacattcc caaatcttta accaagagat
      661 aggtcggaac aagtccgcca ccgaagaaca tggtgaaaac gagaaaggca ttaatccatt
      721 ttttcccgac caaatctttt ttagagagcg ggtaggcagt catgactgag atagccacgg
      781 tcacgaaggc aaaggagaag gaataaaaga gggagttgaa aaatccgcgt aggatagact
      841 tgtctgaaaa tacccgttga taaccttcta ctgtccagtc ctttggatta aagctaatgc
      901 ccttgctgac caaagcctgc ggatccatga aggaagcgac taggatatag accataggca
      961 caactgttat gagaacaaga aatgccagca agattttatt gacaatcagc attcgcttgt
     1021 caaattttgt gtacatttgt gtattcatac gaacctccta taagccttgt ccgtcgttaa
     1081 tgcgtttgac aatcttatta actgcaatca gcaggataat attgataagt gcgttaaata
     1141 agccaaccgc tgttgaataa gagtagtcac ctgacaccaa accgatttta tagacatagg
     1201 ttgaaatgat ttcagaactg gttaggttga gggaggtctg catcaagaag gctttttcgt
     1261 accccacgtt catgatacct ccagctgcta agataaactg aatgaccata actggtttta
     1321 gtgctggtaa gtcaatatgc cagattcgtt ggaaaatatt tgcaccgtct agcttagccg
     1381 cttcaatcaa ggctggatca acattgacca aggtagccgt gtaaagagtc gatgcccagc
     1441 ccattacctg ccaaatacca ctgaggatgt agaggggtct gaagaagtct gggtctgtca
     1501 ggaagttggc ttcaatgcca aacataccta ggatggaatt aaccggtcct cctactgaga
     1561 agaagaggaa aatcataccg acgatgacaa cgactgagat aaagtttgga gcgtagagca
     1621 ccaactgaat ccgtttttta gccttatcgc tcaatagttg attgagcatg atggccaaaa
     1681 taattggtgg aagaaatcct aagagcagac cgtagacact caatttcaag gtgttggcaa
     1741 gtaaggtgcc gaaatttgga gaggaaagaa acttggtaaa ctcttcaaac ccaatccact
     1801 cactaccaag cacccctttt aaaggattat agtctttaaa ggcaatcagt actccgtaca
     1861 taggaatgta acggaagatg aaagtcaaga ccaagcctgg tagtaacatc agtaacaaga
     1921 gtttttgttg cttgattcga tccacgagcc cagcttgttg cagttttgat gttttcataa
     1981 gaactccttt ttgaaacgtt tcatttttct tgtaaaaata aaaccaactg tttattgatg
     2041 attttatttt atcattgtaa ccgttttctg tcaactactt ttttaaaatt tttttgtagt
     2101 ttttccttca agataggaaa tcggtagagt gacttgtagg gggcgctctc ttttctcaat
     2161 aatatcaatt agacaggcaa cggcttcctg ggccatagac tccaaaggtt gacgaatggt
     2221 cgaaagggct aaagattttt cacttgccaa ctgaatacca tcatagccaa tcacctgcac
     2281 atcttctgga acccgtcgtc ccagtttttc caaaacagca agtgtgtcta gggccgtaaa
     2341 atcattgatg gtgaaaatgg cgtcaacctg tggattgtta atcagaaact cttcgacttg
     2401 accaacaaag tcatcatatg gctcttccaa gtccaaaaca tgacaaggaa aacctgcctc
     2461 taaaatccgc ttttcaaaat acaatctacg cttcttggtt tcgttgatgg ttttattgtg
     2521 cccaccgata aaggcaaaat gacgaccacc ttttgcaatc aaggtattcg ctgccagttc
     2581 cgcaccagct cggttatccg aactgacaca ggctatgtcc ttgttttcat aaacacgatc
     2641 gatactgaca aatggaatgt tggaggacag gtaatcttca atcgggctat aagtgatagc
     2701 aataatgccg tccaccttat tttgttgcag catagtcaga taatccagtt ccctttttgg
     2761 accagaaata ttgcaaagga gcaacttgta atttttctta ctcagttcca cttcaacatg
     2821 ataggcaaat tctccaaaaa aaggatgcca aactgtcgga ataatcaagg cgatggtttc
     2881 ggttttgttc ttcttcatcc cacgagcata aacatctgga atgtaattta attcccgaat
     2941 ggctgtattg actttttcaa gcgtactttc cttaatacct ggttcattgt tgataactct
     3001 tgatactgtc ccaacactga cccctgcaac tcgagctaca tctttcatag taattgattt
     3061 ttgttcttcc atggtttacc cctttctttt gaaacgtttc aataatttat actagaatat
     3121 cattgatagc gttttctgtc aagggaaata gacttattag caaataaaaa cacccaaaaa
     3181 gctagaatga ttccaacttt tgggtgccgt tcaaaaatct tttctatttt atttgtacat
     3241 cttcttcatt gggtgttttg caagtacttc agggtgattc tcgagagcag ctctgccttt
     3301 ttccgtcaag gcataacctc ggacggtaaa gtatggagcc agtttttctt ctgtcaagtt
     3361 gtccttgagg gcagtgtcca ggtctgccgc cttcatcttg gacaggcctg tcacaccctc
     3421 tgctttgagg atatttttct tggcagtcga agtgatgtgg tccagcgaat caaaggccga
     3481 ttccacatag gcataaccgt ctgcgatcaa ctgctccatg tgagcaggac cattgatacc
     3541 atacatatac tcaaagtact tggagtaggg cgtcgtcgtt tcaaaagaac ccaggctaat
     3601 ccgccataat aaaataatat cacctggcaa aattccttct tctagccgaa ccatattgcg
     3661 tttgggaacg ggcttggcct ccaaaagcca ggctgccaag tcacgttcgg gggaaatata
     3721 gggtttgacc tcatggtctt tatacatttc ttcaacacga ttctggagtg acatttactt
     3781 tacctcacga attgcgtaat aatttccatc attgtctgca aagcttatta ccttcatgaa
     3841 accaaggtcc atgactggat tcgttgtgac accacgttca acaagttctt cgtaagtttt
     3901 ggctacatct actgatgtca ttaagattga tggtgtacct aagtttaatt ctggctccat
     3961 ctcagcaacc tccgccttat catgaagtac aaattgtacc tcactatctg atttaggtgc
     4021 aataatgtaa gaagtttggg ggccttgcac ttgtttttct acacgttcaa atcccatttt
     4081 ctctttccaa aattgagctg aagcctcaat atcattcaca tataacataa cttgtccgat
     4141 tgattgaatc atcttaaata cctcttttgc gttgtacgaa catgtgttca aaccattata
     4201 cactagatag ggatattctc caaagaaaaa cgactcttag agccgttcaa aacctaggaa
     4261 acgagtcccc tgaaaatgca gggtgcctac atctccctct gctaaaaggg catattcatc
     4321 tgaacgaagg cgaaattcct gccgttttcc ctcccattca aacgttacat aataattggt
     4381 tcgtgctact tcgttaccaa aaacatgggt tcgtttagag acaagcttag ccttgcaaga
     4441 ttctttgggc gcccgattgt tgtggtgcca ttccataaaa tttttaatga tatggaaaca
     4501 aaccgtcgct aatatgattg cggaaaaact gtagaaaagg gccgtatgcc atactaggct
     4561 tccatctggg gcaaacaagt tctcaaacat cattattctc cttttgcatc tgtaaatata
     4621 gtatatcata ttgttgtttt agatacgctt tttatactag ataaaaattg aaaaaatcca
     4681 tatttcatgg tagaatgagc ataattttga acaagacaag aaaggatttt caatgaaaaa
     4741 aatactagtt cttgctctag cttcgagcat cttgctaact gcctgcacaa acaactcgac
     4801 agctaatagc tcatcttcta cagcatctag ctctagtcca agctcgacta cgacagctga
     4861 tacaactgct tctagccaat atgccaaaga tttggcatac gccattaaca atcccaaagc
     4921 tacattcccc caactttcca atgaaattgg tgaaaatgag gctgctgtta aaataaaaac
     4981 aaccgaaggc gacatcacta taaaactctt ccccgagcaa gctccactaa ctgtggaaaa
     5041 ctttctgact catgcgaagg aaggatacta taatggaaca atcttccatc gtgtcattaa
     5101 ggatttcatg attcaaggtg gcgatcctct tggaaatggt actggtggag agtctatctg
     5161 gtccggaaaa gatactacta tcgatgctgg gaatggtttt aaagacgaga tttcagcttt
     5221 cctttataac attcgtagct cactttctat ggcaaacgct ggcgcgggaa caaatggaag
     5281 tcagtttttc atcaatcaaa atacaacaga catgtctagc caactctcta gttctagcta
     5341 tcctggtaaa atcattgacg cttataaaaa cggaggcaat ccaaacttag atggcaagca
     5401 taccgtattt ggtcaagtca ttgaagggat ggacatcgtg gataaaatcg cttcagtcga
     5461 aacagacaat agcgataaac ctaaaacaga tgtaaaaatt gagtctattg aaattctgaa
     5521 agattacacc ttttaaaaat gggttctata atttctgtag tgggtaaaac caccgtggag
     5581 attatggagc ctttttgagt atagaaaaaa agtcccatat gacctataat gaaaagtgct
     5641 caaactatca ttttagaaag actcatatgg aacaactaaa tcttatcaca aattttctca
     5701 gaattaaaga caaaaatatc actatcactg atgaatatga tatgggaact cacttagaac
     5761 tccacggtta cttggattac atggctccta aatgtccaga atgcaaggga caaatggcta
     5821 agtacgacta ccagaaagca tcaaaaattc cctacctaga aactgctggt tatccactcc
     5881 ttatccgcct taaaaagcgt cgtttcaagt gtaaagaatg tgggaaaatg gcggttgctg
     5941 aaactcctct tgttaagaaa aaccatcaaa tatctgtcgc tgttagccag aagatcgc
//
LOCUS       DBNFEG010000134         5949 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.134, whole genome shotgun sequence.
ACCESSION   DBNFEG010000134 DBNFEG010000000
VERSION     DBNFEG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.134"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            3..737
                     /locus_tag="AC1PNG_001665"
     CDS             3..737
                     /locus_tag="AC1PNG_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIP6702156.1"
                     /translation="MLHSRKRIDVKQLEKLTFSSTSTLRRDLIYLEEQGLIKRKHGEV
                     VLNAFNTVELAHSIRETENRSEKKIIAELAKDFVGPGMCIYLDSSTTVYELCPFLSTI
                     DNLIIFTNGLNTAQLLSELSNPTMKIFVTGGEVKHHSASVVNHSMENSLLEHFSIDLA
                     FCSARGIDEEYVYEASFSQAIAKKSIIDKAKETILLIDSSKFRMSGFFKINNLKQYKT
                     IISDKLPDESLLTSAEKYEVEWIANN"
     gene            911..1264
                     /locus_tag="AC1PNG_001666"
     CDS             911..1264
                     /locus_tag="AC1PNG_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein"
                     /protein_id="HIP6702157.1"
                     /translation="MEKRTRPNQLKIRLSDKELAQVREKFGQSRSKSMRHFVLKSILE
                     TSIYEIDMQPFREIQHLLSKTSTNINQIAKKVNTYSVVYKEDIVTIQNEVQHLSKELH
                     KLQNTLYNRTNQGEI"
     gene            1266..2561
                     /locus_tag="AC1PNG_001667"
     CDS             1266..2561
                     /locus_tag="AC1PNG_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HIP6702158.1"
                     /translation="MAVTKIHPIKSTLHFAIDYILDRTKTDSQTLLSTNMCFPTTAHL
                     QFLQTRKEHQVNGSVLARHLIQSFLPGEVDQETAHKIGLELCQKILKDEYEYVLTTHI
                     DKGHIHNHIIFNNVNFKNGKCYQSNKRTYHQIRSTSDRLCKKYKLSVIDEHYQTFRKK
                     YKTRGKSHYEWEQSQNGKGWKPYLQFLIDLNISRSNSWEDFLDRMKKSKCDIKFGKHI
                     SFKGKNASRFTRSKTIGPDYTEERLKERIASKPPHELIDTSTFKDQKGLEFWARKHNL
                     KSMAQGLVELRNLGIYTKDDLEMKIESLADKQISIQDEVKTIDEQMADLEDKMESLHI
                     VQQYQLIYQHYQNNKDNQQYIREYQKEIKAYKMAKNQLLAYDKPLNFRELLSQLDKLQ
                     EKKNTLMTEYSQIKNQFNKLYQHKKNFDSYFETEQNRII"
     gene            complement(2564..3061)
                     /locus_tag="AC1PNG_001668"
     CDS             complement(2564..3061)
                     /locus_tag="AC1PNG_001668"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702159.1"
                     /translation="MILLTLYGMLILAAFWLWLFIARTSVKLACFAIDFCAVFIYTIY
                     LLHQPISSKIANGNTIYFWDVLFGLIAVLIYGFLMVVLSILLPRISKAINFVIVFLGV
                     GIGICLATDFITFLLSIFHSSIEPTYRIQFLTNNLANDILYYVIFFLVSIPVWKKRME
                     YLTGL"
     gene            3227..3919
                     /locus_tag="AC1PNG_001669"
     CDS             3227..3919
                     /locus_tag="AC1PNG_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HIP6702160.1"
                     /translation="MFSPKRLKEKRTELGLSQSEIARQLEISRMSYSAWERGKTKPNH
                     KNLSLLATILKVDESYFESEYSIVQNYLLLTDENKLAAEDYVEQLLTSQSIAENVVSL
                     YSYQVLSDIQLSAGPGEGLYDEFETETVYSEDEYTGFDIATWISGNSMEPVYKDGEVA
                     LIRSTGFDYDGAVYALNWNGSLYIKKLYREEDGFRMVSLNEDYTEKFIPYEDEPRIVG
                     LVVGHFMPVIGG"
     gene            3922..4056
                     /locus_tag="AC1PNG_001670"
     CDS             3922..4056
                     /locus_tag="AC1PNG_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702161.1"
                     /translation="MARQFDDMIYIKGNRIILALDQKDYDVTDHLTELVEELAKLKSR
                     "
     gene            4062..5477
                     /locus_tag="AC1PNG_001671"
     CDS             4062..5477
                     /locus_tag="AC1PNG_001671"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIP6702162.1"
                     /translation="MSYIDYSKEPRSDIAFVDMKSFYASIECVERGLHPLHTSLCVMS
                     RADNTSGLILASSPMFKKIFGKGNVGRAYDLPFDVHTRKFNYYRASISGLPTDVKFVS
                     YIENWAKRTFIVPPRMDLYIQKNLEIQKVFQNYADPTDILPYSIDEGFIDLTSSLNYF
                     VEDKTLSRKDKLDLVSAKIQHDIWEKTGVYSTVGMSNANPLLAKLALDNEAKSTATMR
                     ANWSYEDVETRVWNIPKMTDFWGIGSRTEKRLNKLGIYTIKELANCDPSILKKEFGVI
                     GVQHWFHANGIDESNVHEPYRPKAVGIGNSQVLHKDYTRQSDIELVLREMAEQVAIRL
                     RKRHKKATVVAINVGYSNFENKKSINVQRKINPTNRTLVFQDEVISLFRSKYDGGAVR
                     SIAVRYDGLVDENFAVISLFDDFEESEKEEKLETTIDTIRDRFGFLAVQKASSLLENS
                     RAIARSKLVGGHSAGGLEGLK"
     gene            5474..5824
                     /locus_tag="AC1PNG_001672"
     CDS             5474..5824
                     /locus_tag="AC1PNG_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702163.1"
                     /translation="MIDRSYLPFQVAREYQDRKMAKWMGFFLSEHTSGLEQERNRIDY
                     STELTLADKLLLLNQLYSHELNGILTVPGKIYIGIVKNLTFQSVSFETKEGYISIQIS
                     EILTIDLEVEYESA"
     gene            5811..>5949
                     /locus_tag="AC1PNG_001673"
     CDS             5811..>5949
                     /locus_tag="AC1PNG_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HIP6702164.1"
                     /translation="MNQLEFNKNQLQMDYFSDSYRKFEEDFYRYSKLAVPLTFLTDDL
                     MN"
BASE COUNT         2158 a         1050 c         1043 g         1698 t
ORIGIN      
        1 atattcttca cagtagaaag agaatagatg taaaacaact tgaaaaatta actttctcaa
       61 gtacatcaac tcttcgtaga gatttaatct atcttgaaga acaaggtctc atcaaaagaa
      121 aacatgggga agtggtgcta aacgcattta ataccgtcga acttgctcat agtatcagag
      181 agactgaaaa taggtcagaa aagaaaatca ttgctgagct tgcaaaagat tttgttggtc
      241 cagggatgtg tatttactta gattcaagta caacggttta tgaattatgt ccgttccttt
      301 caacaattga caatctaatt atctttacga atggactcaa tactgctcag ttgctttctg
      361 aactttctaa tccaacaatg aaaatatttg tcactggcgg tgaggtcaaa caccattctg
      421 cctcagtcgt aaaccatagt atggaaaata gtcttcttga acattttagt atagaccttg
      481 ctttttgttc tgcaagggga attgatgaag aatatgttta tgaagcttct ttcagtcaag
      541 ccattgcaaa aaaatctatt attgacaagg ctaaagagac aatattactg attgatagtt
      601 cgaaatttcg gatgtcagga tttttcaaaa ttaacaacct gaaacaatac aaaactatca
      661 tttcagacaa attacctgac gaatccttat taacctctgc cgaaaagtat gaagtagaat
      721 ggattgccaa taattaaatt aaatagaatc ttagcttacc gctctacaat ataaaactgt
      781 tgtagcaagc acagtaagtg ggtacccact tacgataaaa ttaacgaagc agacgccttt
      841 ggctctgctt ttttcgttat attttacttg ttagggagaa ccctaagacc ccgatagaaa
      901 ggaatagaca atggaaaaac gtacccgacc aaaccaatta aaaattcgac tttcagacaa
      961 ggaacttgct caagtgcgag aaaaatttgg acaatctcgg tcaaaatcaa tgcggcattt
     1021 tgttctcaag tctatcctgg aaacttcaat ctatgaaatt gatatgcaac cttttagaga
     1081 gatacaacat cttctatcca agacatcaac taatatcaac caaattgcaa agaaggtcaa
     1141 tacctactca gttgtttaca aggaagacat tgtgacaata caaaatgaag tccagcactt
     1201 gagtaaggaa ctgcataaac tacaaaacac tctctacaat aggacaaatc aaggggagat
     1261 ttgatatggc tgttactaaa attcacccaa ttaagtctac tctccatttt gcaattgact
     1321 acatcctaga taggacgaaa acagatagcc aaacacttct ttccacaaat atgtgcttcc
     1381 caactactgc tcatttacag tttttacaga ctcgaaaaga acaccaagta aatggaagtg
     1441 tcctggctcg acaccttatt caatcctttt taccaggaga agttgaccaa gagacggcac
     1501 ataagattgg cttggaacta tgtcaaaaaa tcttaaaaga tgaatacgag tatgtcttaa
     1561 ctacacacat tgacaagggc catattcaca accatatcat ttttaataat gtcaatttca
     1621 agaatggaaa gtgttaccaa agcaataaac gaacctatca ccagattcgg tcaactagtg
     1681 atcgactttg caagaaatac aagctatccg tcattgatga acattatcag accttccgta
     1741 agaaatataa aactagagga aaatctcatt atgagtggga acaaagtcaa aatggcaagg
     1801 gttggaaacc atacttgcaa tttcttattg acctaaatat ttcccgttca aactcttggg
     1861 aagacttcct agaccgtatg aaaaaatcca aatgtgacat caaatttggt aaacatatta
     1921 gtttcaaagg caagaatgct tcacgattta cacgatccaa aacaattggt cctgactata
     1981 cagaagaaag attaaaagag agaattgctt ccaaaccacc tcatgagctg attgatacgt
     2041 ctacgtttaa agaccagaaa ggattagagt tctgggctag aaaacacaac ttaaaatcaa
     2101 tggcacaagg tttggtcgaa ctaagaaatt taggaattta cacaaaagat gacttagaga
     2161 tgaagattga atcattagct gataaacaaa tttcaatcca agatgaagta aagactattg
     2221 atgaacaaat ggctgatttg gaagacaaaa tggaaagtct acacatagta caacagtacc
     2281 aactcatcta ccaacattac caaaataaca aagataacca acaatatata agagaatatc
     2341 agaaagaaat aaaggcatat aaaatggcaa aaaatcaatt attagcttac gataaacctt
     2401 taaattttag agaattattg tcacagttgg acaagctgca agaaaaaaag aataccctta
     2461 tgacagagta ttctcaaata aaaaatcaat ttaataaact ttatcaacac aagaaaaatt
     2521 ttgactctta ctttgaaaca gagcaaaatc gaatcattta attctatagc ccagttaaat
     2581 actccattcg ctttttccag acaggaattg aaactaagaa aaagattaca tagtataaaa
     2641 tatcattggc aagattatta gtcaaaaatt gtatgcgata ggtcggctca atactcgaat
     2701 gaaagattga taataagaaa gttatgaaat cagttgctaa acaaattcct ataccaacac
     2761 ccaaaaatac aatgacaaaa ttgatggctt tacttattct tggcaataat attgagagaa
     2821 caaccattag aaaaccatat atcaaaactg cgatcaatcc aaataaaaca tcccaaaaat
     2881 aaatcgtatt tccattcgca atcttactag aaattggttg gtgtaacaga taaattgtgt
     2941 agataaatac tgcacaaaaa tcaatcgcaa aacaggcaag ttttacagaa gttctagcta
     3001 taaataacca aagccaaaat gctgctaaga ttaacattcc atacagtgtt aacaaaatca
     3061 ttaaaaactc actcctttca ctacatctag tttactgcat tatactattt ttgtccgctc
     3121 attatattga atttctgacg aaatttttaa agttatactt gtcaaattaa aaaaaaataa
     3181 tttatacttt ttgtaagttt ataaacttac aacgaaagga gtaaccatgt tctctccaaa
     3241 aagactaaaa gagaaaagaa cggaactagg actatctcaa tctgaaatcg cacgacaact
     3301 tgaaattagt cgcatgtcat atagtgcctg ggaacgtgga aaaaccaaac caaaccataa
     3361 aaatctttct ctgcttgcta ctattttgaa agttgacgag tcctattttg aatctgaata
     3421 ttctatcgtt caaaactatc ttttgttaac cgatgaaaac aaattggctg ctgaagatta
     3481 cgttgagcaa ctactaacat ctcaatctat tgcagaaaat gttgtgtcac tatactccta
     3541 tcaagtgctt tcagatatcc aattatctgc tggtcctggt gaagggctgt acgatgaatt
     3601 tgaaacagag actgtctatt cggaagatga atatacgggc tttgatatag cgacatggat
     3661 ttcaggaaac tcaatggaac ctgtttataa agacggcgaa gtcgccttaa ttaggtctac
     3721 tggttttgat tatgatggtg ctgtctatgc cttaaattgg aatggctcac tctacataaa
     3781 gaaactctat cgtgaagaag atgggttcag aatggtctcc ctgaatgaag actatacaga
     3841 gaagttcatc ccttatgaag atgagccaag aattgttggt ctagttgtgg gccactttat
     3901 gcctgtgatt ggaggttagc aatggcaaga cagtttgacg atatgattta cattaaagga
     3961 aatcgcatta ttctagcact cgaccaaaaa gattacgatg taacagacca tctaacagag
     4021 cttgttgaag agttagcaaa attgaaatct aggtgaacac tatgagctat atcgactatt
     4081 caaaggaacc acgtagcgat attgcttttg tcgatatgaa atctttctat gcgtccattg
     4141 aatgcgtgga gagagggctt catccgctcc atacatctct ttgtgttatg agtagggctg
     4201 ataatacttc tggcctcata ctagcttctt ctccaatgtt caaaaaaatt tttggtaaag
     4261 gaaatgtcgg tagagcttac gacctcccct ttgatgtcca cacacgaaaa tttaactatt
     4321 accgtgcaag catttctgga ctaccaacag atgtcaagtt cgtctcatat attgaaaact
     4381 gggctaagcg tacctttatc gtgccaccaa gaatggactt gtatatccaa aaaaatctag
     4441 aaatccaaaa agtatttcaa aattatgctg atccaacaga tattctcccc tattcaattg
     4501 acgaaggatt tattgaccta acttcatcac taaattattt tgttgaagat aagacgctta
     4561 gtcgtaaaga taaattagat ctagtatcag caaaaataca gcacgatatt tgggaaaaga
     4621 ctggagtata ttctacagtt ggtatgagta atgccaatcc acttctagcc aagctggcac
     4681 ttgacaatga agcaaaaagt actgctacca tgcgagcaaa ttggtcctat gaagatgttg
     4741 aaacaagggt ctggaacatt ccgaaaatga ctgacttttg gggcattggt tcacgaacag
     4801 aaaaaagatt gaataaactt ggtatctata ctatcaagga attggccaac tgtgacccat
     4861 ctatcttgaa aaaagaattt ggtgtgattg gagtacaaca ttggtttcat gctaatggta
     4921 ttgatgaaag caatgtacac gaaccctatc gccctaaagc tgtcgggatt ggaaattcgc
     4981 aagtgctcca caaagattac actcgtcaaa gcgatataga acttgtactt agagagatgg
     5041 cagagcaagt tgccattcgc ttacgtaaga gacacaaaaa agcaaccgta gttgccataa
     5101 atgttggata ttcaaatttt gaaaacaaga aatccatcaa tgttcagaga aaaataaatc
     5161 caaccaatcg aacacttgtt tttcaggacg aagttatttc actttttcgc tcaaaatatg
     5221 atggtggtgc agttagaagt attgctgtga gatacgatgg attagtggac gaaaactttg
     5281 cagttatttc actttttgac gactttgaag aaagcgaaaa agaggaaaaa ttggaaacaa
     5341 ctattgatac cattcgtgat cgatttggat ttcttgcagt acagaaagct agttctttat
     5401 tagaaaattc aagggcaatt gctcgaagta aacttgtcgg cggtcattcc gctggcggat
     5461 tggagggatt aaaatgattg accgtagcta tctaccattc caagtagcta gagaatacca
     5521 agatagaaaa atggcaaaat ggatgggatt tttcttgtca gagcatactt caggtttaga
     5581 acaagaacgc aatcgtattg attattcaac tgaattgaca ttagctgata aactactcct
     5641 actcaatcaa ctatatagtc atgagttaaa tggtattctg acagttcctg gaaaaattta
     5701 cattggaata gtaaaaaatt tgacattcca atctgtttcg tttgaaacta aagaaggcta
     5761 catcagtatt caaatctcgg aaattcttac aatagatttg gaggtggagt atgaatcagc
     5821 ttgagtttaa taaaaatcaa ttacaaatgg attattttag tgatagctac cgaaagtttg
     5881 aagaggattt ctatcgctac tcaaaattag ccgttccact tacctttctt acagatgatt
     5941 tgatgaatt
//
LOCUS       DBNFEG010000135         5822 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.135, whole genome shotgun sequence.
ACCESSION   DBNFEG010000135 DBNFEG010000000
VERSION     DBNFEG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.135"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1582
                     /locus_tag="AC1PNG_001674"
     CDS             <1..1582
                     /locus_tag="AC1PNG_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative polysaccharide biosynthesis protein"
                     /protein_id="HIP6702165.1"
                     /translation="AWLTAGNILSRLLGVAYVIPWYIWLGEYRAEANALFSMGYQIYA
                     NFLLISTAGLPTAIAKQVAKYNVLGKEEVSLYLVREFFKLMLVFGAVFAGVMYLSAPW
                     LADASGSKEKLLPVMYSLVPPLFIFPAMSILRGFFQGRHDMKPYAISQLAEQLVRVIW
                     ILAATFMIMKLGSGDYLEAVVQSTFAAFVGMIASVGILVYTLWKQGYLGKLLHAKKQK
                     ISLDTGQLIKETVRDAIPIIILGLTIQLLQFIDQVTFIRVMERITDYSNSELLELYSY
                     MAANPSKITMMIIGISLSLGSVAIPLITEKFVKKDLKAASHLVADNLQLLFIFTIPAI
                     VGTVLLAGPLYTIFYGPSEPIAITLFIWNLFLILPLGLYSVISVVIQAIFENRRAIYY
                     FLIGMLVKIVLQVPMIYVFKVYGSFISTIFGLGIMLYLFYRRIDKVLHIDERLVIKDI
                     ATISWISIVMGLIVWGIEFVLNLILPANGYVSSFIHLAVAGGAGMLVFVILTLKTRQL
                     DRLIGSRAQSLRRKLRLG"
     gene            1749..2483
                     /gene="pyrH"
                     /locus_tag="AC1PNG_001675"
     CDS             1749..2483
                     /gene="pyrH"
                     /locus_tag="AC1PNG_001675"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIP6702166.1"
                     /translation="MMKPKYERILIKLSGEALAGERGVGIDLKTVQEMAKEIQEVAES
                     GIQIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLKQLGVDTRVQ
                     TAIAMQSVAEPYIRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDANAVKFNELTHREVISRGLKIMDATASTLSMDNDIDLVVFNM
                     NEPGNIKRVVFGEPIGTTVSNSSEEK"
     gene            2617..3174
                     /gene="frr"
                     /locus_tag="AC1PNG_001676"
     CDS             2617..3174
                     /gene="frr"
                     /locus_tag="AC1PNG_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIP6702167.1"
                     /translation="MSKEIIAKAQERMNQSHQSLAREFSHIRAGRANASLLDRISVEY
                     YGSPTPLNQLAGITVPEARVLLITPFDKSILKDIERALNASDLGLTPQSDGTVIRLVI
                     PALTEETRKNLAKDVKKVGENSKVAIRNIRRDAMDEAKKAEKAKEITEDELKTLEKDI
                     QKVTDDAIKTIDKMTADKEKELLEV"
     gene            3306..4160
                     /gene="cvfB"
                     /locus_tag="AC1PNG_001677"
     CDS             3306..4160
                     /gene="cvfB"
                     /locus_tag="AC1PNG_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HIP6702168.1"
                     /translation="MNDLLAQYIVGLVTDENDQFYFVQKEGRTFALSKDEGEHSLGQS
                     VKGFAYTDMKQKLRLTTKEVGASRTSFGWGTVTEVRKDLGVFVDTGLPDKQVVVSLDI
                     LPEIKELWPKKGDQLYVKLDVDKKDRIWALPAFQEDFQKMAGPAYDNMQNQNLRAIVY
                     RLKMNGTFVYLPDNNMLGFIHPSERFAEPRLGQVLEARVIGYRAVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSAPEDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DSFGTELV"
     gene            4170..5090
                     /locus_tag="AC1PNG_001678"
     CDS             4170..5090
                     /locus_tag="AC1PNG_001678"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HIP6702169.1"
                     /translation="MLVYIAGSGAMGCRFGYQLSKTNHEVILLDNWEEHIEAIRENGL
                     KVTGDVEETVHLPIMKPAEATREADFIILLTKADQLPKMLRDIKPIIGPTTKVLSLLN
                     GLGHATTMRRYVPDESIMVGVTMWLAGLKGPGHAHLEGPGSISVQHILGDEQSVADII
                     EMLNEAGLNVTYDNHVVSAIWQKACVNGVMNPTCTILDCTIGELFGTEAGLNLVQGIF
                     KEFIAVAKSEADGIDEEAIWKYVIDASKKASNHYPSMHQDLVQHGRKTEIDYLNGAVV
                     FKGKRAGIPTPYCQMITDMIHAKESMLGLR"
     gene            5100..5555
                     /locus_tag="AC1PNG_001679"
     CDS             5100..5555
                     /locus_tag="AC1PNG_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HIP6702170.1"
                     /translation="MLIEAETIEDYLAALPENRKEAVERLHQVIVEQLPAGFEVGILG
                     AMINYYVPLAAYPDGYHCTPGEPLPFLALASQKAHIALYHMGIYMDQELNDWFVAAYQ
                     AQVPTKLDMGKSCVRMKNPKNIPYELIGDLVSKMSMERYIELYEKNHRK"
     gene            5561..5776
                     /locus_tag="AC1PNG_001680"
     CDS             5561..5776
                     /locus_tag="AC1PNG_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HIP6702171.1"
                     /translation="MRRSFYSWLMTQRNPKSNEPVAILADYAFDESDFPKQSDNFDEV
                     SRFLEESASFAFSMSDFDAIWEDYLGH"
BASE COUNT         1645 a         1001 c         1404 g         1772 t
ORIGIN      
        1 ggcttggttg actgctggaa atattcttag tcgcctgctt ggtgttgcct atgttattcc
       61 ctggtatatt tggttagggg aatatcgagc tgaagccaat gccctcttta gtatggggta
      121 tcaaatctat gccaactttt tattgatttc aacagctggt cttccgacag ctattgctaa
      181 acaagtagct aaatacaatg tcttgggtaa agaagaggtg tcgctttact tggtcagaga
      241 attcttcaag ctcatgttgg tctttggagc tgtctttgca ggggtcatgt atctaagtgc
      301 tccgtggttg gcagacgcat cggggtcgaa ggaaaagtta cttccggtca tgtatagtct
      361 agtcccgcct ctctttatct tcccagccat gagtatcttg cgtggttttt tccaaggtcg
      421 gcacgatatg aagccctatg ctatcagcca gcttgcagag cagctggtac gggttatctg
      481 gatactggca gcaacattta tgattatgaa gttgggtagc ggtgactatc ttgaagcggt
      541 tgtccaatct acctttgcag cttttgtcgg gatgattgct agtgttggga ttttagttta
      601 tactttatgg aaacaaggtt accttggtaa gttacttcat gctaagaaac aaaagatttc
      661 tttggataca ggtcaactta ttaaggaaac tgttcgagat gcgattccaa ttatcatttt
      721 aggtctaacg attcaattat tgcagtttat tgaccaagtc acctttattc gtgttatgga
      781 aagaattacg gattacagta attcggaact tttggaactt tattcatata tggctgccaa
      841 tccaagtaag attacgatga tgattatcgg gatttcactg agtttgggaa gtgttgctat
      901 tccattgatt acagagaaat ttgtcaagaa agatttgaag gcggcgtctc atttagtggc
      961 ggataatcta caattactct ttatattcac tataccggca attgttggaa cagtcctgtt
     1021 agctgggcca ctctatacga ttttttacgg accgtcagag cctatcgcca ttactctatt
     1081 catttggaac ttatttttaa ttttacctct gggactatat tctgtcatta gtgtggtcat
     1141 tcaagcgata tttgaaaata gaagagccat ttattatttc ttgattggga tgctagtgaa
     1201 aatcgtttta caagtaccaa tgatctatgt ctttaaggta tatggtagct ttatctcaac
     1261 gatatttggg ttgggaatta tgctgtacct cttctataga cgaattgaca aggttctgca
     1321 tattgatgaa agactagtga taaaagatat tgcgaccatt agttggattt ctatcgtaat
     1381 gggactcatt gtatggggga ttgaatttgt cttgaatctt atccttccag caaatggcta
     1441 tgtatctagc tttatccact tggctgttgc aggtggagca ggtatgcttg tctttgtcat
     1501 cttgacattg aagactcgtc agctagatcg tttaattgga agtcgggctc agagtttacg
     1561 gaggaaattg cgcctaggct agtttgtttt aaaaatctct attttctact attgaaaagc
     1621 tgggaaaaag aggtgtttta gaatgtgaga aggaaagttc gttgaggatt ttcctttttt
     1681 cttggagatt tcagtataaa aaaaacggtt tttgtggtag aatagtaggg ataaaaaaga
     1741 ggagagagat gatgaaacct aaatacgaac gtattctaat caaactatct ggtgaagcct
     1801 tggctggtga acgtggtgtc ggtattgacc ttaaaactgt tcaggaaatg gcaaaggaaa
     1861 ttcaggaagt cgcagaatca ggtattcaga ttgcccttgt tattggtggt ggtaaccttt
     1921 ggcgtggaga acctgctgct gaagcgggta tggatcgagt tcaagcagac tatacaggca
     1981 tgttggggac tgttatgaat gctcttgtaa tggctgattc ccttaaacag cttggtgtgg
     2041 atacacgtgt gcagacagcg attgcgatgc agtcagttgc agagccttat attcgtggtc
     2101 gtgctcttcg tcatcttgaa aaaggtcgca ttgttatttt tggagctggt attggttctc
     2161 cgtacttctc aacggatacg acagcggctc ttcgtgctgc agaaattgaa gcggatgcga
     2221 ttttgatggc taaaaatggt gtggatggcg tctacaatgc tgatccgaag aaagatgcta
     2281 atgcagttaa attcaatgaa ttgacccacc gtgaagtgat tagtcgtggt ttgaaaatca
     2341 tggatgcgac agcatcaact ctctcaatgg acaatgatat tgatttggtc gtgttcaata
     2401 tgaatgaacc aggaaatatt aaacgagttg tctttggtga gccaatcggt actactgttt
     2461 caaattcttc agaagaaaaa taaatcatat cgtcatttag tttttctttt attacttcgt
     2521 taattcgcct tgcctaactt cggttatacc tgtggctcgt tgcttagtac taaaagtaaa
     2581 ctaaaagact ataaaaaata caaagaagga agtcttatgt ctaaagaaat tattgctaaa
     2641 gcgcaagagc gcatgaacca atctcatcag agtttggcac gtgaattcag ccacattcgt
     2701 gctggccgtg ccaatgctag tttgttggac cgtatttcag ttgagtacta cggttctcca
     2761 acgccattga accagttggc tggtattaca gtgccagaag cgcgtgttct tttgattact
     2821 ccgtttgata agtcaatctt aaaggatatt gagcgtgctt tgaacgcttc agatcttggt
     2881 ttgacaccgc aatctgacgg tactgttatc cgtttggtta ttcctgccct tacagaggaa
     2941 actcgtaaga acttggcgaa ggatgtgaaa aaagtcggtg aaaattcaaa agttgccatc
     3001 cgtaatatcc gtcgtgatgc catggatgaa gctaaaaaag ctgaaaaagc aaaagaaatc
     3061 acagaagacg agttgaagac gcttgaaaaa gatattcaaa aagtaacaga tgatgctatc
     3121 aagacaattg ataaaatgac tgccgacaaa gaaaaagaat tgttggaagt ttaatcaaca
     3181 cgtttttaga ctacaaaaat agatcatcac tgggctgtct aacttccagt ggtgaaccaa
     3241 taagaaatct aatgggggga gttagacaag acttccccca ctttttaatc agaaagaaga
     3301 aaatcatgaa tgatttatta gcacagtata tcgttggttt agtgactgac gaaaacgatc
     3361 agttttactt tgtccaaaaa gagggtagaa cctttgctct ttctaaggat gaaggagaac
     3421 atagtctagg tcaatctgtt aagggatttg cctataccga tatgaaacaa aaactccgtt
     3481 tgacaactaa ggaagttggg gcgagtcgta ccagctttgg ttggggaact gtcacagagg
     3541 tacgtaagga tttgggtgtc tttgtggata caggtctgcc agacaagcaa gtggttgttt
     3601 ccttggacat tttgcctgaa atcaaggaac tctggccgaa aaaaggggat cagttgtacg
     3661 tcaaattaga cgtcgataag aaggatcgca tttgggctct gccagctttt caagaagatt
     3721 ttcagaaaat ggctggacct gcttatgaca atatgcaaaa ccaaaatctg cgagcgattg
     3781 tctatcgttt gaaaatgaat ggtacttttg tttatttgcc agacaacaac atgcttggtt
     3841 ttatccaccc aagtgaacga tttgcggaac cacgtctggg tcaggttttg gaggcgcgtg
     3901 tcatcggcta ccgtgcagtt gatcggacct tgaacttgtc tctcaagcca cgttcatttg
     3961 agatgctgga aaatgatgcc cagatgatat tgacttatct ggaaagcaat ggaggtttca
     4021 tgaccttgaa cgataagtct gctccggaag acatcaaggc cacctttggt atttccaaag
     4081 ggcaattcaa gaaagctctt ggtggtttga tgaaggctgg aaaaattaaa caggattctt
     4141 ttggaacaga gttggtctag ggggcgtcta tgctagtata tattgctggt agtggggcaa
     4201 tgggttgtcg ttttggttat caattgtcta agacaaatca tgaagtgatt ttgctggata
     4261 attgggaaga acatatcgag gcaattcgag aaaacggctt gaaagtgact ggagatgtag
     4321 aggaaacagt acacttaccc atcatgaaac cggccgaagc aacgagagaa gcagatttta
     4381 tcattctatt gacaaaggct gatcagttgc caaagatgtt gcgggatatt aaaccgatta
     4441 tcggtccaac taccaaggtt ttgagtctct tgaatggttt ggggcatgcg acgaccatgc
     4501 gtcggtacgt gccagatgaa agtattatgg ttggggtgac catgtggttg gcgggactaa
     4561 aggggcctgg acatgctcac ttagaaggtc cgggttctat ctccgttcag catattcttg
     4621 gtgatgagca atcagttgcg gatattattg aaatgctgaa tgaagctggg ctaaatgtga
     4681 cctatgacaa tcatgttgtc agtgccattt ggcagaaagc ctgtgtcaat ggtgtgatga
     4741 atccaacttg taccatttta gactgtacaa ttggtgaatt atttggtacg gaggctggac
     4801 tgaatctagt acaggggatt ttcaaagaat ttattgctgt tgctaagtct gaggcagatg
     4861 gaattgacga agaagctatt tggaaatatg ttattgatgc ttcgaagaag gcttccaatc
     4921 actatccatc tatgcaccag gatttggtcc aacatggtcg caagacagaa attgattatc
     4981 tgaatggagc agttgttttt aagggcaaac gtgcaggtat ccctacgcca tactgtcaga
     5041 tgataacgga catgattcat gccaaagaga gtatgctggg gttgagatag gagaaagtca
     5101 tgctgattga agcggaaacg attgaagact atctggctgc cttgcctgaa aatcggaagg
     5161 aagcagttga acgactccat caagtgattg tggaacagtt accagctggt tttgaagtag
     5221 gaatcttggg agctatgatt aactattatg tgccgcttgc tgcctaccct gatggttatc
     5281 attgcacacc gggagagccc ctgcctttcc ttgccttggc atcacaaaag gcacacatcg
     5341 ccctctacca catgggaatc tatatggacc aagaattgaa tgattggttc gttgctgctt
     5401 atcaagcaca ggtgcctacc aaactggata tgggcaagtc ctgtgttcgt atgaaaaatc
     5461 caaagaatat tccttatgaa ttgattgggg atttagttag taagatgtct atggagcgct
     5521 atatagagct ctacgaaaaa aatcatagaa agtagatgta gtgagaagaa gtttttattc
     5581 ttggttgatg acccagcgca acccgaaaag taatgaacca gtagctatct tggcagatta
     5641 tgcctttgat gaatctgatt ttcctaagca atcagataat ttcgatgaag tcagtcgttt
     5701 tttggaggaa tcggccagct ttgccttttc catgtctgat tttgatgcga tttgggaaga
     5761 ttatttggga cattgatgga taaaatagta caaaatagcc ttgtttgggc tattttctac
     5821 ta
//
LOCUS       DBNFEG010000136         5816 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.136, whole genome shotgun sequence.
ACCESSION   DBNFEG010000136 DBNFEG010000000
VERSION     DBNFEG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.136"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(66..2237)
                     /gene="nrdD"
                     /locus_tag="AC1PNG_001681"
     CDS             complement(66..2237)
                     /gene="nrdD"
                     /locus_tag="AC1PNG_001681"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIP6702172.1"
                     /translation="MNVQENVLPSIELLVLKRDGRTVSFDQDKIFSALRRANQELEHP
                     VSEAGLKIVLEAVLAEIGRRFEKDVQIYEIQTVVEQELLKAHLYDLAELYIQYRTRRD
                     FRRHQATDINFEIHKLLSKDQSVVNENANKDADVFNTQRDLTAGIVGKSIGLKLLPAH
                     VANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLAQGFKIGNADVESPKSIQTATAQ
                     ISQIIANVASSQYGGCSADRIDEVLAPYAELNYQKHLKDAKDWVLPDKQEEYARAKTQ
                     KDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSWFEREIQKAILNIRIKGLGREG
                     RTAIFPKLIFTVKRGLNLEPDSPNYDIKQLAIECATKRMYPDMLSYDKIVELTGSFKV
                     PMGCRSFLQGWKDENGQDVTSGRMNLGVVTVNLPRIAMESAGDKDKFWEIFVERMAIA
                     KDALVYRVERVKEATPANAPILYQYGAFGKRLAKTDAVDEVFKNRRATVSLGYIGLYE
                     VATVFYGGEWETNPEAKDFTVDIIKKMKALVEDWSDEYGYHFSVYSTPSESLTDRFCR
                     MDTEKFGIVKDITDKEYYTNSFHYDVRKNPTPFEKLDFEKIYPAVGASGGFIHYCEYP
                     VLQQNPKALEAVWDYAYDRVGYLGTNTPIDHCYACDFEGDFEPTERGFKCPNCGNSDP
                     KSVDVVKRTCGYLGNPQARPMVKGRHKEISARVKHMNGSSL"
     gene            complement(2543..3286)
                     /locus_tag="AC1PNG_001682"
     CDS             complement(2543..3286)
                     /locus_tag="AC1PNG_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIP6702173.1"
                     /translation="MIKIRNLVKEFSGQKVLDGIDMDIEKGQVIALVGPSGAGKSTIL
                     RSLNYLEQADAGSIEIDGFTVDFTNISKEEILTLRRKLAMVFQQFNLFERKTALDNVK
                     EGLKIVKRMDDASATALAKEELRKVGLSERENHYPRHLSGGQKQRVALARALAMQPDI
                     LLLDEPTSALDPELVGEVERSIADAAKTGQTMILVSHDMNFIYQVADKVLFLENGRIL
                     EQGTPEELFHHPKEERTREFFASYSNPQF"
     gene            complement(3286..4089)
                     /locus_tag="AC1PNG_001683"
     CDS             complement(3286..4089)
                     /locus_tag="AC1PNG_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIP6702174.1"
                     /translation="MIGVLGITGPAWYQGFLEWLPDGKLFSIRAVFDALPSIIERLPI
                     TIGLTLAGAFFGLILGLVFAIVKINRVRFLYPVQAVFVSFLRGTPILVQLMLTYYGIP
                     LVLRGLNQEYGLDWNINAIPAAVFAITAFAFNEAAYMSESIRAAIQAVSPGEIEAARS
                     LGMTSGQVYRRIIIPNAAVIATPSLINTLIGLTKGTSLAFSAGIVEVYAQAQIQGGAD
                     YRYFERYISVSLVYWALSVLIESFGRFLENKMAIQAPESVDISGVGGDR"
     gene            complement(4275..5126)
                     /locus_tag="AC1PNG_001684"
     CDS             complement(4275..5126)
                     /locus_tag="AC1PNG_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIP6702175.1"
                     /translation="MKLRTILSLAGITALATVALAACSSTSNSSNTSSSEADKKETIV
                     MGTVGTTKPFSYRDENGELTGFDIELARAIFDGSDQYELTFETTDGSGQFPGLDSGRF
                     DLLGNNTSYTKERGEKYLYSYPTASTPSVLAVPKDTDIQSYDDIGGHSTQVVTGTTTA
                     AQLEEYNTQHTDKPVDINYTGENITQILTNINDGKYDFKIFDAPTVNAIIADQNLTNL
                     KTIELEAAEKPFIYYVFSSDNTALQSFVNDRIKALNEDGTIAKLTEQFFGKDYAPTAE
                     ELAVPNE"
     gene            complement(5159..>5816)
                     /locus_tag="AC1PNG_001685"
     CDS             complement(5159..>5816)
                     /locus_tag="AC1PNG_001685"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIP6702176.1"
                     /translation="NEKPVAEGIPIMNLSLSRNFADGIKRAGGLPFYIPLSGETDVVD
                     YVSAIDKLLLTGGQHVLPQFYGQEKLIESDDYFKERDEFELKLVHEALRQEKPILGIC
                     RGMQLFNVARGGTLKQAVEGHRSLPFDLIHQVECKEATPLASIFGQRVAVNSIHSQAI
                     ENLGQGLEAIVHSVDDGIIEAVRSMDETRFLGVQWHPELLIDQSGGNQKLFDYFVQTL
                     "
BASE COUNT         1719 a         1399 c         1068 g         1630 t
ORIGIN      
        1 tttgtttata ataaacttcc acagctaact ggaactgtgg aagctctaca tagtctgttt
       61 attacttata agctcgaccc attcatgtgt ttcacacgcg ccgaaatttc cttgtgacgg
      121 ccttttacca tcggacgggc ctgtggattg cctagataac cacaagttcg tttaacaaca
      181 tccaccgact taggatcgct attcccgcaa tttggacact tgaagccacg ctccgtcggc
      241 tcaaaatcac cttcaaaatc gcaagcgtaa caatggtcaa ttggagtatt ggttcccaag
      301 taaccaacac ggtcataagc atagtcccaa acagcttcca aggcctttgg attttgttgc
      361 agcacagggt attcacagta atggataaat ccaccactgg cacctacagc aggataaatt
      421 ttctcaaagt ccagtttttc aaacggtgtt ggatttttgc gtacatcata atggaaactg
      481 ttagtgtaat attctttatc cgtaatgtcc ttaacgatgc caaatttctc tgtatccata
      541 cggcagaagc ggtctgtcag gctttcagat ggtgtcgaat aaactgagaa atgataacca
      601 tattcatccg accaatcttc aaccaaggct ttcattttct tgataatatc caccgtgaaa
      661 tcttttgcct ctgggttggt ctcccattcg ccaccgtaaa atactgttgc gacttcatac
      721 aaaccaatgt accccaaaga tacggttgct cgacgatttt taaagacttc atctactgcg
      781 tctgtcttag ccaagcgttt gccgaatgcc ccatactgat acaaaattgg tgcattggca
      841 ggtgtcgcct ccttgacccg ctccacgcgg taaaccaagg catccttagc aatggccata
      901 cgttctacaa agatttccca gaacttatcc ttatctccag cagactccat ggcaatccgt
      961 ggcaaattga cggttacgac acccaagttc atccgaccag atgtcacatc ctgcccattt
     1021 tcatccttcc aaccttgaag gaaagaacgg cagcccattg gcaccttgaa agaacctgtc
     1081 aactcaacaa tcttgtcata agaaagcata tctggataca tacgcttggt cgcacattca
     1141 atcgccaatt gcttgatgtc atagtttgga gaatctggct ccaagttcaa tccacgctta
     1201 acggtgaaaa tcaacttagg aaagatagct gtccgacctt ctcgccctag acccttgata
     1261 cgaatgttca aaatcgcttt ttgaatttct cgctcaaacc atgatgtccc taacccaaaa
     1321 ccgagtgagg taaagggtgt ttgtccattg gaagtaaaga gggtgttgat ttcatactcc
     1381 aaagactgca tagcatcata gatgtctttt tgcgtttttg cacgcgcata ttcttcttgc
     1441 ttgtctggca atacccaatc cttggcgtct ttcaaatgct tttggtaatt gagttccgca
     1501 taaggcgcca aaacttcatc gatacggtca gctgagcaac caccatattg actagaagcc
     1561 acattggcaa tgatttgtga aatctgagcc gtagctgttt ggatagactt cggactttct
     1621 acatcggcat ttcctatctt gaagccttgt gccaacattc ctttgaaatc aatcaaacaa
     1681 cagttggtca ttggtgtata agggctgtaa tccaaatcat gataatggat gtcacccttt
     1741 tgatgagcat tggctacatg agctggtaat aatttaagac cgattgactt gccgacaatc
     1801 cccgctgtca ggtcccgttg agtattgaac acatccgcgt ccttattggc attttcattg
     1861 accacagact ggtccttgct caacagctta tgaatctcaa aattaatatc ggtagcctga
     1921 tgacggcgaa aatcacggcg tgttcggtat tgaatataaa gctccgccaa atcatataaa
     1981 tgagccttta acaattcctg ttcaacgacc gtttgaattt cgtaaatttg aacatctttt
     2041 tcaaatcggc gaccaatttc agccaaaaca gcttctaata caatttttaa acctgcctct
     2101 gaaacaggat gttctaattc ttggtttgcc cgccgaagag cagaaaaaat cttatcctgg
     2161 tcaaaggata ctgtccgtcc atcacgtttc aaaactaata attctatact cggtaagaca
     2221 ttttcttgca cattcatctg acaaacctcc aaaattcttc acttctatta gtgtagcact
     2281 tcacaaaaca aaaatcaata tcttgtgtaa aaaaatatta aacaacctat ccatgcacta
     2341 tatataggtc ttgaaaattc tatcaattgg aaaacaactg tgccaaattt caccactcga
     2401 caaggatgaa ttactagatt gaaatatgac aacagattgg gcaaaggtct aacttctcta
     2461 tcagaaaacc taaaaaacag ccctctaaca gggctggctc tatgtattga aaataggcta
     2521 gggcgggtca ccacatgcct atttaaaatt gaggatttga ataagaggcg aaaaattctc
     2581 tagtccgttc ttctttagga tgatggaaaa gctcctcagg tgtcccctgc tccaggatac
     2641 ggccgttttc tagaaataat accttatccg ccacttggta aatgaaattc atgtcatgac
     2701 taactaaaat catcgtttgt cctgtcttag ccgcatcggc aatggaacgt tctacttctc
     2761 caaccaactc tggatcaagc gccgaagtcg gctcatctag tagcaaaatg tctggttgca
     2821 tggcaagagc gcgtgcaaga gccacacgtt gcttttgacc acctgacaga tgtcttggat
     2881 aatggttctc acgttctgac agaccgacct ttctcaattc ttctttggca agggcggttg
     2941 cagatgcgtc atccatccgc ttgacaattt taagcccttc cttgacgtta tcaagagctg
     3001 tctttcgttc aaacagatta aactgctgaa agaccatagc cagtttgcgg cgtaaggtca
     3061 atatctcctc ttttgaaata ttggtaaaat caactgtaaa accatcaatt tcgatactcc
     3121 ccgcatctgc ctgctccaga tagttcagac tacgcaaaat ggttgatttt cctgctccag
     3181 atggtccaac aagggcaata acctgccctt tttcaatgtc catatcgatt ccatctagta
     3241 ctttttgtcc tgaaaattcc ttgactaaat tgcgaatttt aatcattagc gatcacctcc
     3301 aactccagaa atatctactg attcaggggc ctgaatagcc attttatttt caaggaaacg
     3361 gccgaaagac tcaatcagaa cagagagcgc ccagtagacc aaagagacag aaatgtaccg
     3421 ctcaaagtag cgatagtctg ccccgccttg aatctgtgcc tgtgcataga cctcaacgat
     3481 acccgcactg aaggctaggg aagttccttt agtcagacca atcaaggtgt taatcaacga
     3541 cggagttgca atcacagccg cattaggaat gatgatccga cgataaacct ggccagaagt
     3601 catacctaag gaacgagccg cctcaatctc ccctggacta acagcctgaa tcgcagcacg
     3661 aatagactca ctcatatagg ctgcctcatt aaatgcaaag gctgtaatcg caaagacagc
     3721 tgcaggaata gcattgatat tccaatcaag accatattcc tgattgagcc ctctaagaac
     3781 caatggaatt ccgtagtagg tcaacatcag ctgcaccaag ataggagtgc ctcgcaaaaa
     3841 actaacaaaa actgcctgaa cgggatataa gaatcgcaca cgattaatct ttacaatcgc
     3901 aaaaaccaag cccaatatca aaccaaaaaa agcacctgcg agcgtcaagc caatcgtaat
     3961 cggtaaacgt tcaataatgg agggtagggc atcaaaaaca gccctaatac taaatagctt
     4021 accatccggt aaccactcta aaaaaccttg ataccatgcc ggtcctgtta ttcctaatac
     4081 accaatcata ctcttctcca aacatttaaa aatgacagat tttctgccgt atactctatt
     4141 ttatcatgta accacccaca tcaaccttat atatatttta tggctgtgat aagtaatctt
     4201 aatgattata aaaagacaat gaaaaagaaa cagcccactt ggactgctcc ctcctgtcac
     4261 aatactatcg actcctattc attaggaaca gctagttcct ccgccgttgg agcatagtct
     4321 tttccgaaga attgctctgt caatttcgca atggtcccat cttcgttcaa agccttgata
     4381 cggtcgttga caaagctttg tagagccgta ttgtccgaag aaaatacata gtaaataaac
     4441 ggtttttctg ctgcttcaag ctcgattgtt ttaagatttg tcaaattttg atctgcaata
     4501 atagcattaa ccgtaggggc atcaaaaatt ttaaaatcat acttgccatc atttatattg
     4561 gtcaaaatct gtgtgatatt ttcaccagta tagttgatgt cgactggttt atccgtatgc
     4621 tgagtattgt attcctctag ctgagcagct gttgttgtcc ctgtaactac ctgtgtagaa
     4681 tgaccaccaa tatcatcata agactgaata tccgtatctt ttggcacagc taaaacggat
     4741 ggtgtagagg ccgttggata agaatagagg tatttttcac cacgctcttt tgtataggaa
     4801 gtattattcc ctaacaaatc aaagcgtcct gaatccaagc cagggaattg accgctacca
     4861 tctgttgttt caaaggtcaa ttcatactgg tcagaaccat caaagatagc tcgagccaat
     4921 tcaatatcaa accctgtcaa ttctccattc tcatctcgat aagaaaaagg cttggtagtc
     4981 ccaacagttc ccattacgat ggtttctttt ttatccgctt ctgaagaact agtgtttgat
     5041 gagtttgagg tagacgagca agctgccaaa gcaactgtcg caagagctgt aataccagcc
     5101 agtgatagaa tagttcttaa tttcatagta attctccttt tgtataagca agtcagacct
     5161 acaaggtctg cacaaaataa tcaaatagct tctgattgcc gccactttga tcaatcagaa
     5221 gttccggatg ccactggaca cccagaaaac gtgtctcatc cattgatcgg acagcctcaa
     5281 taataccatc atccacagaa tgcacaatcg cttctagccc ctgacccaga ttctctatcg
     5341 cctggctgtg aatagaattg acagctactc tctgaccaaa aatactagcc agaggcgttg
     5401 cctctttaca ttctacctgg tgaatcagat caaaaggcaa ggaccgatgc ccctctaccg
     5461 cttgtttgag agttcctcca cgtgcaacat tgaaaagctg catccccctg caaatcccca
     5521 aaatcggttt ttcctgtctc aaggcttcgt ggaccaattt cagttcaaat tcgtcacgtt
     5581 ccttgaaata gtcatcgctt tcgatgagtt tttcttgacc gtaaaactga ggtaatacat
     5641 gttgaccacc cgtcagtaat aacttatcaa tcgccgacac atagtcgaca acatccgttt
     5701 caccagacaa ggggatataa aaaggaaggc cccccgctcg cttgattcca tctgcaaagt
     5761 tacgagacaa cgataaattc ataatcggaa tcccctctgc gacaggcttt tcatta
//
LOCUS       DBNFEG010000137         5740 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.137, whole genome shotgun sequence.
ACCESSION   DBNFEG010000137 DBNFEG010000000
VERSION     DBNFEG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.137"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1081
                     /locus_tag="AC1PNG_001686"
     CDS             <1..1081
                     /locus_tag="AC1PNG_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIP6702177.1"
                     /translation="TLLAVLNWSSIYGAIQQLLGSMNSLFIGAIFAFILNVPMKKLED
                     QFEKISFLKKSKRSLAIVGVLIGFALIVTGLVVIVLPTLVTTVSELVSVSSTAIPKSV
                     NALTNFLESNGLLSGQIGDQIASFLDQLKNLTLISSLVTPILSGLVSNVTGIFSNTMT
                     LIMAFFFTLAILGSKEHLQAMTRKFLQATLPEKAVRVVNYIGEVIVDTYDRFLMSQIV
                     EACIIGTLVFVSYSLSGIPYASMAGILAGVLSFVPYIGPFSACLISALFVAVQNPLLA
                     LWSIALFQIIQLIEGNVIYPRVVGQSVGLPTLFTLAAALIGGNLFGLLGMVFFTPIFA
                     VVYRLVREWVASRLKKQGELLAESE"
     gene            1153..1644
                     /gene="nrdR"
                     /locus_tag="AC1PNG_001687"
     CDS             1153..1644
                     /gene="nrdR"
                     /locus_tag="AC1PNG_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIP6702178.1"
                     /translation="MRCPKCQSLKSSVIDSRQAEDGNTIRRRRSCDQCGQRFTTYERI
                     EEKTLVVVKKDGTREQFSREKIFNGIIRSAQKRPVSTDDIDEVVNRIEQKVRAQGDSE
                     IESDVIGNLVMEELVELDEITYVRFASVYRSFKDVGELENLLKQMISKGSKVKPGKKD
                     ETK"
     gene            1631..2782
                     /locus_tag="AC1PNG_001688"
     CDS             1631..2782
                     /locus_tag="AC1PNG_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation/membrane attachment
                     protein"
                     /protein_id="HIP6702179.1"
                     /translation="MKPNDLFAYIKISPFTPDIVSLSQCYQPIIGFDALALYYYFYSF
                     ADQGQGRYKWTVILNHMDFGMNRLEKALDVLTAMGLLELYRADELTGLALQAPLTVKS
                     FLAKPLYKNLLARKIGESSMENLLVHQPNQEQNISKTFSQVFTMDGQAPVVFEPKHDF
                     DWSAFKALMAKDKLLFQDEAEDIIALSYIAEQAGWTWLETYRQAKATAIGQTISTKRL
                     QQSRQSLPTENANLTAQEQAIVREAKSKSSLTFLSFIKEQRKAAVTGTERKCLTDLAN
                     LGLLDEVINVLVLYTFNKVDSANLNEKYAMKLGNDFSYKGSVSAEAAVLYLRELKTGQ
                     VVTKSSQPQKTNVPEWSKEEVQQGQTQEGQAKLAALYRELEEMENKGGS"
     gene            2782..3681
                     /gene="dnaI"
                     /locus_tag="AC1PNG_001689"
     CDS             2782..3681
                     /gene="dnaI"
                     /locus_tag="AC1PNG_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HIP6702180.1"
                     /translation="MKSVQDRLSQTTNPSPKSYQQLYQEIVSDPEVAAFIKKEGLTQQ
                     EITLSISKFLEYISQRDLFVKQDEAYIAKGYQPVLVMNEGYADVSYLETEELVEYRRL
                     EAIKNRIQLINMPASLKNVTVADIDKSDENRVEVMLAIADFVKRFEEKPKGLYIYGNF
                     GIGKSYLMAYLANLLSKTHLQSTTMLHYPTFVVDIKNAIKDGSVKERIDEIKMAQVLV
                     LDDIGAEQHSPWVRDDVLQVILQYRMQENLPTFFTSNFSFDDLERHFASGKSGDETWQ
                     AKRVMERIRYLARDLHLKGNNRR"
     gene            3678..4391
                     /locus_tag="AC1PNG_001690"
     CDS             3678..4391
                     /locus_tag="AC1PNG_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HIP6702181.1"
                     /translation="MNETIDLMLNHTSVRRFTEEPIEAEHLQAIISAGRAASSWKNFQ
                     SYSIIVVQLEEKKQALYDLVPQPAILQAQAILVFVGDHNRASKAAQLHGSDFDAKGTE
                     NLLISSVDASLAGQNALLAAESLGYGGVFIGMIRHKAMAIAELFNLPDYTYPIFCIAL
                     GRPAQNHPVKPRLASETIVFQEEYVEQGVEVIQAYDQVQTAYAGARQTETWSERMVAQ
                     FGQAEQPETKSILEKNKLL"
     gene            4410..5720
                     /gene="der"
                     /locus_tag="AC1PNG_001691"
     CDS             4410..5720
                     /gene="der"
                     /locus_tag="AC1PNG_001691"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIP6702182.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRKFSLIDTGGIDDVDAPFMEQIKHQAEIAMDEADVIVFVVSGKEGVTDADEY
                     VSRILYKTNKPVILVVNKVDNPEMRNDIYDFYSLGLGDPYPVSSVHGIGTGDVLDAII
                     ENLPVQEAEENPDIIKFSLIGRPNVGKSSLINAILGEERVIASPVAGTTRDAIDTHFT
                     DPEGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIERSDVILMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKKWEDDIRDQFQYLSYAPIIFVSALTKQ
                     RLHKLPEMIKAISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVVFVNEEELMHFSYMRFLENQIRKAFVFEGTPIHLIARKRK"
BASE COUNT         1595 a         1141 c         1364 g         1640 t
ORIGIN      
        1 gacattgtta gctgttctca actggtctag tatttatgga gctattcaac aattattagg
       61 tagtatgaat tcgcttttta ttggagctat ttttgccttc atcctcaatg ttcccatgaa
      121 aaaattagaa gatcagtttg aaaaaatttc ttttttaaag aaatccaagc ggtctttggc
      181 aattgttgga gtattaattg gatttgcttt aattgtgaca ggactggtgg tgattgttct
      241 tcctacattg gtgacgactg tttcagaact ggtttcggtt agtagtacag ctattccaaa
      301 atcggtaaat gccctaacta attttcttga atcaaatggt cttttgtcgg gacaaatcgg
      361 agaccagatt gccagctttt tggatcagtt gaagaattta acacttattt caagtttggt
      421 cacaccgatt ttatctggct tggtgtccaa tgtgacgggg attttttcga acactatgac
      481 gttgataatg gctttcttct tcactctagc gattttaggg agcaaggaac acttgcaggc
      541 gatgacgaga aagttcttgc aggctacctt gccagagaag gctgtcagag tagtgaacta
      601 tattggagaa gttattgttg atacttatga ccgattcttg atgagtcaga ttgttgaggc
      661 gtgtatcatt gggacacttg tctttgttag ttattcgctc tcaggaattc cttatgccag
      721 catggctggt attttggctg gggtcctatc atttgtacct tatatcggtc ccttttcagc
      781 gtgtttgatt agtgctcttt ttgtggcggt acaaaatccg ctcttggcac tctggtctat
      841 cgcccttttc cagattattc agttgattga agggaatgtg atttacccgc gcgtagttgg
      901 tcaatcagtt ggtcttccga ctctctttac cctagcagca gccttgattg gtggtaattt
      961 gtttggccta cttggtatgg tcttctttac cccaattttt gcggttgtct atcgcttggt
     1021 tcgtgaatgg gttgccagtc gattgaagaa gcaaggtgaa ttattggcag aaagtgagta
     1081 attctcgctt tcttttattt ttgtcatttt tatgctatac tgaagtgata aagtttatgt
     1141 aggagaaaga gtatgcgttg tccaaaatgt cagagtttga aatcaagtgt cattgatagt
     1201 cgtcaggctg aagatggcaa taccatccgt cgtcgtcgtt cctgcgacca atgtggtcaa
     1261 cgttttacca cctatgaacg gattgaagag aaaactctag tcgttgtgaa aaaagacgga
     1321 actagagagc agttttcacg tgaaaagatt ttcaatggga ttattcgttc tgctcaaaag
     1381 cgccctgtgt caaccgatga tattgatgaa gtggtgaatc ggattgaaca gaaggttcga
     1441 gcacaaggtg atagtgaaat agaatccgat gtcattggga atttggtaat ggaagaattg
     1501 gtcgaattgg atgaaatcac ctatgtgcgt tttgcatcgg tttatcgttc tttcaaagat
     1561 gttggagagc tagaaaattt gcttaaacag atgatttcca aaggaagtaa ggtaaaaccg
     1621 ggaaaaaaag atgaaaccaa atgatttatt tgcctatata aaaatcagcc cgtttacacc
     1681 tgatattgtc agtcttagcc aatgctacca gccgattatc ggttttgatg cattagcgct
     1741 ttattattac ttttatagtt ttgccgatca agggcaagga cgttacaaat ggactgtcat
     1801 tctcaatcac atggattttg ggatgaatcg tttggagaag gctctggatg ttctgactgc
     1861 catggggctc ctagaactct atcgtgcaga tgaattaaca ggtttggcgc tacaagcccc
     1921 tctgaccgta aaaagttttc tagcgaaacc cttgtataag aacttgttgg caaggaagat
     1981 tggggagtca agcatggaga atctgcttgt tcaccaaccc aatcaagaac agaatatctc
     2041 aaaaacattt tctcaggtct ttaccatgga tgggcaggct ccagttgttt ttgaaccgaa
     2101 acatgatttt gactggtctg ccttcaaggc attaatggct aaagataagc ttctttttca
     2161 ggatgaagct gaggatatta ttgcgcttag ttacatagca gagcaggcgg ggtggacctg
     2221 gttggaaacc tatcgccagg ctaaagcaac agctattgga cagaccatct cgaccaaacg
     2281 cttgcaacag tcacgtcagt cattgccaac agaaaacgcc aacctcactg cccaagaaca
     2341 agccattgtt cgtgaagcga aatcaaaatc tagcctaacc ttcctgtcct ttatcaagga
     2401 gcaacgcaag gcagctgtga caggaacaga acgaaaatgc ttgacggatt tggccaattt
     2461 gggtctgctt gacgaagtca tcaatgtctt ggtcctctat acctttaaca aggtggactc
     2521 agccaatcta aatgaaaaat acgctatgaa gctgggcaat gatttttcct acaagggaag
     2581 tgtcagtgca gaggcagctg ttctttacct aagagagttg aagacaggtc aagttgttac
     2641 caagtcgagc cagccacaga aaaccaatgt gccagagtgg agtaaggaag aagtccagca
     2701 aggacaaacg caggaaggtc aagccaagct ggcggctctt tatcgtgaat tagaagaaat
     2761 ggaaaacaaa ggaggtagct gatgaaatca gttcaagaca ggttgtcgca aacaaccaat
     2821 ccaagtccaa aatcttatca gcaactctat caggagatag tgagtgatcc tgaagtcgct
     2881 gcttttatca aaaaagaagg tctgacccag caggaaatca ccctctccat ttctaagttt
     2941 ttggaatata tcagtcagcg tgacctcttt gtcaagcagg atgaagccta tattgcaaaa
     3001 ggctatcaac cggttttagt catgaacgag ggctatgcgg atgtgtccta tcttgagaca
     3061 gaggaattgg tcgaataccg tcgtttggaa gccatcaaga atcgtatcca gctcatcaat
     3121 atgcctgcga gtttgaagaa tgtaacagtg gcggatattg ataagagtga tgaaaatcgt
     3181 gtggaagtca tgctggctat tgcagatttt gtcaaacgat ttgaggaaaa accaaaaggg
     3241 ctctacatct atggcaattt cggtattgga aaaagttatt tgatggctta tttagccaat
     3301 ctcttgtcca agacccatct tcaatcaacc actatgcttc attatccaac ctttgttgtc
     3361 gatataaaaa atgccatcaa agatggttct gtcaaggagc gaattgatga aatcaagatg
     3421 gctcaggtct tggtgctaga tgacatcgga gcggagcaac atagtccttg ggtacgtgac
     3481 gatgtcctgc aagtcattct tcaataccgc atgcaggaaa atctgccgac cttttttacc
     3541 tccaatttct cctttgatga tttggagcgt catttcgcct ctggcaagtc tggtgatgag
     3601 acctggcagg ccaaacgggt tatggagcgg attcgctatc tggctcgcga tttgcatttg
     3661 aaaggaaata accgccgatg aatgaaacca tagacttgat gctgaatcat acttccgtcc
     3721 gtcgttttac ggaggagcct attgaggcgg aacatttgca agctattatt tcagctgggc
     3781 gagctgcgtc tagctggaag aatttccagt cttattccat cattgtggtg cagttggagg
     3841 agaaaaaaca ggcactctat gatttggtgc cccagccagc tattttacag gctcaggcca
     3901 ttctggtttt tgtgggcgac cataatcgtg ccagcaaggc ggctcaactc catggttcag
     3961 actttgatgc caaaggaaca gagaatctcc tgatttcttc agttgatgcg agcctagcag
     4021 ggcaaaatgc ccttttagca gctgaaagcc taggttacgg tggcgtcttt atcggtatga
     4081 ttcgccataa ggccatggca atagcagagc tgttcaactt gccagattat acctatccaa
     4141 ttttctgtat tgccttgggt cgaccagccc aaaaccatcc tgttaaacca cgtttggcaa
     4201 gtgaaaccat tgtctttcag gaagaatatg tggagcaagg cgtggaagta atccaagcct
     4261 acgaccaagt tcagacagcc tatgcaggtg cccgtcagac agaaacttgg tctgagcgaa
     4321 tggttgccca gtttggtcag gctgaacaac cagaaacaaa gtctatctta gaaaagaata
     4381 aattattgta aactagaaga ggaggagcta tggctctacc aactatcgcc atcgttggcc
     4441 gtcccaacgt tggtaaatca acactattta accgtattgc gggggaacgt atttctatcg
     4501 tagaagacgt ggaaggtgtg acccgcgacc gtatttatgc tactggtgag tggctcaacc
     4561 gtaagttttc cttgattgat acaggcggta ttgatgatgt ggatgcacct ttcatggagc
     4621 aaatcaagca tcaggctgag attgccatgg atgaagcgga cgttatcgtc tttgtcgtat
     4681 caggcaagga aggtgtgacg gatgctgacg agtatgtatc gcgtatcctt tacaagacca
     4741 ataagccagt cattttggtt gtcaacaagg tcgataaccc tgaaatgcgt aatgacatct
     4801 atgatttcta ttcgcttggt cttggggatc cctacccagt atcatctgtg catggtatcg
     4861 gtacagggga tgtcttggac gctatcattg aaaatctgcc agtccaagaa gcagaagaaa
     4921 atccagatat tatcaagttc agcttgattg gtcgtccaaa cgttggaaaa tcaagtttga
     4981 ttaacgctat tttgggtgaa gagcgcgtga ttgcgtcgcc tgttgctgga acgactcgtg
     5041 atgccattga tacgcacttt acagacccag aaggtcagga atttaccatg attgataccg
     5101 cagggatgcg caagtctggt aaggtctatg aaaatacgga gaaatactct gttatgcgtg
     5161 ccatgcgtgc cattgaacgc tcggatgtta tactcatggt catcaatgca gaagaaggta
     5221 ttcgtgagta tgacaagcgc atcgcaggtt ttgcccatga agctggtaag ggaatgatta
     5281 tcgtggtcaa caagtgggac acgttggaaa aagacaacca caccatgaag aaatgggaag
     5341 atgatatccg cgaccagttc caatacttgt cttacgcacc gattatcttt gtatcagcct
     5401 tgaccaagca acgcttgcac aagttgcctg agatgattaa ggccatcagt gaaagccaga
     5461 atacccgtat tccatcggct gttctcaacg atgttatcat ggatgccatt gccattaacc
     5521 cgacaccaac tgacaagggt aaacgtctca agattttcta tgcgactcag gttgcgacca
     5581 aaccaccgac ctttgtggtc tttgtcaacg aagaagagct catgcacttc tcctatatgc
     5641 gtttcttgga aaatcaaatc cgcaaggcct ttgtatttga agggacaccg attcatctga
     5701 ttgcacggaa acggaagtaa agagggaaac ctcttttttc
//
LOCUS       DBNFEG010000138         5724 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.138, whole genome shotgun sequence.
ACCESSION   DBNFEG010000138 DBNFEG010000000
VERSION     DBNFEG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.138"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            114..3212
                     /locus_tag="AC1PNG_001692"
     CDS             114..3212
                     /locus_tag="AC1PNG_001692"
                     /inference="COORDINATES: protein motif:HMM:NF019517.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp58-like family protein"
                     /protein_id="HIP6702183.1"
                     /translation="MEKGLLATDYSPSYEDYRSEIATYKRTAEESSAELSRQIQLADG
                     KAVEAKTYAQQTAEGFKTRLESLESYKDAEGTRANQYLTASRTETAKQLSAERAAIAT
                     NYVAKSTYDENVRGTTLKLNEIKTTADTAKQNLATYQNTVDRKLEELTTSTQTLDGKI
                     NTASAKVDTVAGQIRTEISEVEGKIPITAGTRNLLKGTKELTDIYWTSNVTSDYYQGF
                     RIARTAPSAATYIDTYRASTTIVPDATEYIISFYAKSSINGTPINNHFYSPNTTTRSE
                     SNTGYIGKGTDGLAIINLTTTWQRYWIKWTQTPSNTKKNVIIGRNFSANNATVEIAGV
                     ALYEGSLNKDWSPAPEDFANELSSVKTTITQTASGVEQLSTSLTTTDSKVTTAEAKIR
                     QLISDVSSKVSQTDYNTLTGRVDSAETAITQNATEISKRLTKTQVDKAITDKGYQTKS
                     DVDSNITGRGYITNSALQPYALATTVQNLVRETADSFSRTISETKALIPTSVGGRNLI
                     RGSSEMIIGSGRWQDGTFRKSGTGNIKTINISNPPVPNVTKGIEVEIASATECGIAQD
                     TLFLPKGTYTFTVWVKGPTGATGRIDTFNGSGSKSKVFKLTGGWDKVTVTNTSTVDET
                     KNVGYVYLQNSPGYPAKMHLTAPKLEAGTIPTDYTLAIEDLATVTALHDVKDTVSSHT
                     RTIGAVGETGSILDNVSKVTQTAAGLVQEVSGDNGLKTQVSQLAGSYAIKNLTSSGKV
                     LNQLNLNKDGSVKIDGSLVQITGRTYIQDGVITSAKIAGLDAGKVTTGYLASARIKAN
                     SIDGSKIAFDEAFFNRLVANQAYLKKLFAKDAFLTAVQAVSLSASQIIGGLMRATNGA
                     MEIDFNNSAIDMFKDGAIRFHSGSNAIYRQSTDGIHTAFVHFDTTSKGGIYASLGSTS
                     SRDGINSKSSGRFAGIRVARTSERSDSHVASQDAVELYGDTIFFGHGFEGGGFSMHTT
                     LTGGRTISLNKMHDALINLCRCWIHLRNEGFNLNSSAFRKSLDTEIHNAQQLYNTIER
                     IT"
     gene            3212..3427
                     /locus_tag="AC1PNG_001693"
     CDS             3212..3427
                     /locus_tag="AC1PNG_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702184.1"
                     /translation="MNQELINQALRLTNNDLMTKLSEEMTTKNLLAVQLTEAQQTIAN
                     LRAEITELTKQLDEATKPEEIIEQKGE"
     gene            3430..3774
                     /locus_tag="AC1PNG_001694"
     CDS             3430..3774
                     /locus_tag="AC1PNG_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702185.1"
                     /translation="MTQTTNNTLLNLEETTQPFDLATALRYMKEHGEFIRCKSATQDF
                     YMYRDVQKRPAIVNGRRKFVDVETIWAFNQWGSTTTTINIADMLNEEYWIMKFDEHGN
                     PDWSDPTVGAEV"
     gene            3784..4206
                     /locus_tag="AC1PNG_001695"
     CDS             3784..4206
                     /locus_tag="AC1PNG_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIP6702186.1"
                     /translation="MMLSKTPPHPAGMFEFLRELIATEDGLVLFLLGLIVTMEIVDFL
                     SGTFAAMINPDIEYKSKIGINGLIRKMMGIILLTVLIPMSVLLPEQTGVAFLYTIYVG
                     YLILTFKSLIENYGKAKGDTSIFENVTAAFEKLIGKGK"
     gene            4303..5040
                     /locus_tag="AC1PNG_001696"
     CDS             4303..5040
                     /locus_tag="AC1PNG_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PlySs2 family phage lysin"
                     /protein_id="HIP6702187.1"
                     /translation="MTTVNEALNNVRAQVGSGISVGNGECYALASWYERMISPDATVG
                     LGAGVGWVSGAIGDTISAKNIGSSYNWQANGWTVSTSGPFKAGQIVTLGATPGNPYGH
                     VVIVEAVDGDRLTILEQNYGGKRYPVRNYYSATSYRQQVVHHITPPGTITQSAPNLAG
                     SRSYRETGTMTVTVDALNVRRAPNTSGEIVAVYKRGESFDYDTVIIDVNGYVWVSYIG
                     GSGKRNYVATGATKDGKRFGNAWGTFK"
     gene            5105..5482
                     /locus_tag="AC1PNG_001697"
     CDS             5105..5482
                     /locus_tag="AC1PNG_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR2 nuclease fold domain-containing protein"
                     /protein_id="HIP6702188.1"
                     /translation="MKYNVKISQKIKEIVGLETSATDILIHEENLEKHMLKSRHRKMI
                     KYIPDVATILDSPDFVGQNKNVKTESFEVIKVLADNVLVAVKLDKKNDYFFVASVYDI
                     TDSKLNHMKRNGRIKAFDKIEDK"
     gene            5496..>5724
                     /locus_tag="AC1PNG_001698"
     CDS             5496..>5724
                     /locus_tag="AC1PNG_001698"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702189.1"
                     /translation="MNDNEVRKAPDALERVPEMLDTPPNRYHCISKTPQQTLGAFFVV
                     DTNVGSNRPSSMSVSLLKNAMSTDILQNCRFC"
BASE COUNT         1819 a         1180 c         1306 g         1419 t
ORIGIN      
        1 caaaaactga ctggaaacga tttattttta aatttcgtgc aaaaaaatcg ggtaaagaat
       61 tagtatattt tttaaatcga aatggtatcg aagtcgatat aaaaaatata aagatggaaa
      121 aagggctttt agcaacagac tactctccat cttacgaaga ctatcgctct gaaatcgcaa
      181 catacaaacg taccgccgag gaatccagtg cagagttgtc ccgtcaaatc caattggcag
      241 atggcaaggc tgtcgaggct aagacctacg cccagcagac tgctgaaggc tttaagactc
      301 gtctggagag cctagaaagc tacaaagatg cggaaggtac acgagccaac caatatttga
      361 ctgccagtcg aaccgaaact gccaaacagt tatctgccga acgtgccgcg attgctacta
      421 actatgtggc taagtctacc tacgacgaaa atgtcagagg aacaacgcta aagcttaacg
      481 agattaagac gaccgctgac actgccaagc aaaatctagc gacttatcaa aatacagtcg
      541 ataggaagct agaagaattg accacaagca ctcaaacact tgacgggaaa atcaacactg
      601 caagtgccaa ggtcgatacc gtggcgggac agatacggac tgagattagc gaggttgagg
      661 ggaagatacc tatcacagcc ggaacacgta acttattaaa aggcactaaa gagctgactg
      721 atatatattg gacatcgaat gtaacaagtg attactatca aggatttagg atcgccagaa
      781 cagcaccatc ggcagcaaca tatattgaca cttatagagc gtcaacaaca attgttcctg
      841 atgccacgga atacataatc agtttttacg caaaatcctc gattaatggc acaccgatta
      901 acaaccactt ttatagtcca aacacgacaa caagaagcga aagtaacaca gggtacatcg
      961 gtaaagggac agatggtctt gccattatca atctgacaac aacttggcaa cgctactgga
     1021 taaaatggac gcagacacca tctaacacta agaaaaacgt gataatcgga cgtaatttta
     1081 gcgcaaataa tgcaacagtt gaaattgctg gggtggctct atacgagggt agcttaaata
     1141 aggattggtc accagcgcct gaagattttg cgaatgaact atcatctgtt aaaaccacaa
     1201 tcactcaaac cgcatccggt gtcgagcaat tatcgactag cctgactacg actgatagca
     1261 aggtcacgac tgctgaggct aaaatccgac agttaattag cgatgtgtca agcaaagtat
     1321 ctcaaacgga ctacaatacg ttgactggcc gagtcgatag cgctgaaaca gcgattactc
     1381 aaaatgcgac agagattagc aagcgattga caaagacgca ggttgataaa gctatcacag
     1441 ataaaggcta tcagacaaag tctgatgttg acagcaacat cacaggtcgt ggctatatta
     1501 ctaatagtgc attgcaacct tatgccttgg ctacaacagt ccaaaacctc gtcagagaga
     1561 ctgcggatag ttttagccgg acgataagtg agactaaagc gctgattccg acatcggtag
     1621 gtggacgaaa tctgattaga ggctcgtctg aaatgattat aggcagtggt cgttggcaag
     1681 atggcacatt tcgaaaatct ggtacaggta atattaagac aatcaacatc agtaatccgc
     1741 ctgtcccaaa tgtgactaaa ggaatagaag ttgaaattgc gtcagctacc gaatgtggga
     1801 ttgcccaaga cacactattt ttaccaaagg gcacctacac attcactgtt tgggtcaagg
     1861 gcccaactgg agccactgga cgaattgata cgtttaatgg tagcgggtca aaatcaaaag
     1921 tgtttaaatt gacaggcggc tgggataaag taacagtcac taatacatcg acagtagacg
     1981 aaactaaaaa tgtcggttat gtatatctac aaaattctcc cggttaccct gcgaaaatgc
     2041 atttaacagc tccaaaactt gaggcaggaa ccattccgac ggattataca ctagccattg
     2101 aagacttggc cactgtcact gccctacacg atgtcaaaga tacggtatca agccacaccc
     2161 gtactatcgg cgctgtcggt gagacaggca gtattttgga caacgtcagc aaagttactc
     2221 agactgcagc gggtttggtg caggaagtat ctggtgataa tggactcaag acacaagtca
     2281 gtcaattggc tgggtcttat gcgattaaaa atttgaccag ctcaggcaag gtacttaacc
     2341 agctcaacct aaataaagac ggttcggtca agattgacgg cagtctcgta cagattactg
     2401 gtaggactta catacaagac ggtgtgatta catccgctaa gattgcaggc ttagatgctg
     2461 gtaaggttac gacgggttat ttagcatctg cacggattaa agcgaatagt atcgatgggt
     2521 caaaaattgc attcgatgaa gctttcttca acaggttagt cgcgaaccaa gcctacctga
     2581 agaagttatt tgccaaggat gcatttttga cagcagtgca agctgtaagt ttgtcagcga
     2641 gccaaatcat aggtggattg atgagggcaa ccaatggagc tatggagatt gactttaata
     2701 attcggctat tgatatgttt aaagatggcg cgataagatt tcactcaggc tctaatgcta
     2761 tctatcgtca aagtacagat ggcatccata cagcgtttgt gcatttcgac acaacatcca
     2821 agggggggat atatgcttct cttggttcga catcatcaag ggatggtatt aacagtaagt
     2881 cttcaggtcg ttttgccggt atccgagtgg ctcggacctc tgagcgttca gattctcatg
     2941 ttgcgtcgca ggatgctgtt gaactgtacg gagatacaat tttcttcggt catggtttcg
     3001 aaggcggagg tttctcgatg catacaactc tgacgggcgg ccgaacgatt agtctaaaca
     3061 agatgcatga cgcactcatt aacctttgtc gctgctggat acacttgcga aacgaaggtt
     3121 ttaatttgaa tagttcagca ttcaggaagt cgttagatac agaaattcat aacgcacaac
     3181 aactttataa tacaatcgaa aggattacat aatgaaccaa gaactaatca accaagcgct
     3241 acgcttgact aacaatgacc tcatgactaa attgtcagag gaaatgacga ccaagaactt
     3301 gctcgctgtg caactaaccg aagcgcagca gacgattgcc aatttgcggg cagaaattac
     3361 tgagttaaca aagcaactgg acgaagctac taaaccagaa gaaatcattg aacaaaaagg
     3421 agaataatca tgactcaaac tactaacaac acattgctta atttggaaga aacaacacaa
     3481 ccatttgacc ttgcgactgc actccgctac atgaaagaac atggtgaatt tatccgctgc
     3541 aagtcagcga cacaagactt ttatatgtat cgcgacgtgc agaaacgtcc tgcaattgtg
     3601 aatggtcgtc gcaagtttgt tgacgtggaa acgatttggg catttaacca gtggggtagt
     3661 actacgacga ctatcaacat tgctgatatg ctcaacgaag agtattggat tatgaaattt
     3721 gacgaacatg gcaacccaga ctggtcagac ccgacagttg gggcggaggt gtaatacatg
     3781 gttatgatgt tatctaaaac gccaccacat cctgcaggaa tgtttgaatt tttacgcgag
     3841 ctaatcgcaa cagaggacgg tctagtcctc tttttgcttg ggcttattgt taccatggaa
     3901 attgtcgatt ttttatcagg tacattcgct gctatgatta acccagacat cgaatacaag
     3961 tctaaaatcg gtattaatgg cctgattcgt aaaatgatgg gaattatttt gcttaccgtg
     4021 ttgataccaa tgtcggtctt gttgcctgaa cagacgggtg tggcatttct ctacacaatc
     4081 tacgttggct atctcatctt gacctttaag agcttgatcg agaactacgg caaagccaag
     4141 ggcgacacct caatatttga aaacgtgaca gcagcttttg aaaagttgat tggtaaaggc
     4201 aaatgaaacc aatcaaagca atcttgatta ttattgtttt gggagctatg acgcctattt
     4261 tgtgggtgct agctcctttc tattttgaaa aggaagataa atatgacaac agtaaatgaa
     4321 gccttaaata acgtccgtgc ccaagtcggg tctggcatat cagtaggtaa cggcgaatgc
     4381 tacgctttgg ctagttggta cgagcgcatg attagtccgg atgcaactgt cggacttggc
     4441 gctggtgtgg gctgggtcag cggtgcaatc ggcgatacaa tctctgccaa aaacatcggc
     4501 tcatcataca actggcaagc taacggctgg acagtttcca catctggtcc atttaaagca
     4561 ggtcagattg tgacgcttgg ggcaacacca ggaaaccctt acggacatgt ggtaatcgtc
     4621 gaagcagtgg acggcgatag attgactatt ttggagcaaa actacggcgg gaaacgttat
     4681 cccgtccgta attattacag cgctacaagc tatcgtcaac aagtcgtgca ccacatcaca
     4741 ccgcctggaa cgattacaca gtcagcaccc aaccttgcag gctctcgttc ctatcgcgag
     4801 acgggcacta tgactgtcac ggtcgatgct ctcaatgttc gcagggcgcc aaatacttca
     4861 ggcgagattg tagcagtata caagcgtggt gaatcatttg actatgatac tgtcatcatc
     4921 gatgtcaatg gctatgtctg ggtgtcttac ataggcggca gcggcaaacg taactacgtt
     4981 gcgacgggcg ctaccaaaga cggtaagcgt ttcggcaatg cttggggtac atttaaataa
     5041 agtttcagcc cagcatttgc tggggattat gatttgtgaa aaggaatgtg gtataataaa
     5101 aatcatgaaa tacaatgtaa aaatcagtca aaaaatcaag gaaatagtag ggttagaaac
     5161 ttctgcaacc gatatcctga tccacgaaga aaatcttgaa aaacatatgt taaaaagcag
     5221 acatcgaaag atgatcaaat atatcccaga tgttgctact attttggatt cgccggattt
     5281 tgttggtcaa aataagaatg tgaagacaga gagttttgaa gttatcaaag tccttgcaga
     5341 taatgttctg gtcgctgtca aactagataa gaaaaacgac tatttctttg tggcttcggt
     5401 ttatgacatc acagattcca aattaaatca catgaagaga aacggtcgca tcaaagcatt
     5461 tgacaaaata gaagataagt agtaaactaa atacaatgaa tgataacgag gtcagaaagg
     5521 ctcctgacgc actcgagaga gtacctgaga tgttggatac gccgcccaac cgctatcatt
     5581 gtatttcaaa aacccctcag caaacgctgg gggctttttt tgttgtcgac acaaatgtcg
     5641 gtagcaacag accgtcatca atgtcggtca gtttgttaaa aaatgccatg tccacggaca
     5701 ttttgcaaaa ttgccgtttt tgca
//
LOCUS       DBNFEG010000139         5608 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.139, whole genome shotgun sequence.
ACCESSION   DBNFEG010000139 DBNFEG010000000
VERSION     DBNFEG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.139"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(238..1515)
                     /locus_tag="AC1PNG_001699"
     CDS             complement(238..1515)
                     /locus_tag="AC1PNG_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIP6702190.1"
                     /translation="MNKQTYKEILTIAFPAMGENLLQMLMGVVDSYLVASIGIVALSG
                     VSLANNILAVYQAVFIALAAAVSSRLAQALGEGGSEGIGGTASESVKFTVFIGLLLGI
                     FSVLAGPIVLASLGAESEVVQAGGLYLILVGGGAPFWGLMASLSAILRTLGEPRFPMY
                     ISLLSNVLNALFSAFAVFVLHAGVAGVAIGTVLSRLIGCSLLWSRLPISLKPWTWSFD
                     KELLRLALPATGERLMMRVGDVVVVALITSLGTATVAGNAIGETLTQFNYMPALGIAT
                     ATIILTAKHRQDKAMVRHIFRTSFGLSLLFMFLVAGVTYFAGPFLISLYTKDIQAVQA
                     SQTVLFYAMLGVPFTAGTLIMTALWQGLGNARLPFYATTIGMWLVRIGIAYLLITFFK
                     IGLSAIWIATILDNAFRAGVLFFQYRYSKQESI"
     gene            complement(1521..2234)
                     /locus_tag="AC1PNG_001700"
     CDS             complement(1521..2234)
                     /locus_tag="AC1PNG_001700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP6702191.1"
                     /translation="MKKLQVKDLMVTGAFAALYFVCVGLGTLLSLVFDRSGNMMYAPA
                     GAALLAGPVYMLLIAKVGKPGSISLVGANGSGKTSLARYLVGLAEDKKSRISWQGQTL
                     SSRQRLEKTAFVMQDVRLQLFAESVERELTLGRKNRAVDEHLVARFGLSDLLERHPVS
                     LSGGEQQRVMIVASLLADKEIFIFDEPTSGLDLRRMQQVASALVDLKMKNKLVLLISH
                     DEELLNLVCDKIVDIKQLK"
     gene            complement(2235..3896)
                     /locus_tag="AC1PNG_001701"
     CDS             complement(2235..3896)
                     /locus_tag="AC1PNG_001701"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6702192.1"
                     /translation="MDKKEMLTRVLIPRLLQSMKHLLPRIAVAVCFAVLGQVVTIAIP
                     VTLVYLAFAALAGNPAPLWTLGILILLALLRGAFRYGEHYFGHYVAFHTLAAYRRLIF
                     AKLRALAPGKLDRQDSGSLLKMIGEDIEALEVFFAHTIAPICTGILVAFGLTVYFGLS
                     SWGLALLALVTYALLAIAIPNRFAQQLEPLLKEQNASRKGYVSYFIESLKSMKDLMQF
                     QQTDARFAKLTQKSQEVNGQERKVAQTNFMQYAVSFLVVGLSIMCFAWLTFDLVGSQS
                     LDLATGVALLVSFTSSFAPFLELSRLPLGFKRAMNAGRNIYALLDEKEAERTGELVEV
                     RVTDIAIEQLDFDYDDRETGLYRNLSVQFEQGGIIGLVGESGAGKSTLMKLIMRWYDW
                     QQGQIRLSGLDSRQVDKAHLQGAFAYVPQVPQIFRQTIRENLLLGRTDVSDETIMDLA
                     EKCHMKERILAAPQGLDTLVEASDFSAGEGQRLELMRALLKNADCYIFDEPTSNLDSL
                     NEARFIQLVKEHCQGMVFLISHRSSTMACADTIFYLEDGQLVKEK"
     gene            complement(3889..5601)
                     /locus_tag="AC1PNG_001702"
                     /pseudo
     CDS             complement(3889..5601)
                     /locus_tag="AC1PNG_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
BASE COUNT         1703 a         1461 c         1122 g         1322 t
ORIGIN      
        1 tcgctatttc tagcttgaat atagtatata gctactaact taaacgaaac tgaaagattt
       61 cttagatttt tctaaagcaa tttctcatac ttattttctt aaaacttaac acgaaaacag
      121 ccgaccgcgg tcgactgttc tctggagatt atatgaaaaa gaaattcgct atttctagct
      181 tgaatatagt atatagctac aaacttaaaa gaaactgaaa attttcttag ccttatctta
      241 aattgattct tgttttgaat acctatactg aaaaaatagc acccctgctc ggaaggcatt
      301 atctaaaatc gttgcaatcc aaatggctga caaaccgatt ttaaaaaatg ttataagtaa
      361 ataagctatg ccaatccgca ctagccacat accgatagtt gtggcataaa agggtaatct
      421 agcatttccc aaaccttgcc aaagagctgt cataataaga gtgcctgctg taaagggaac
      481 tcccaacatg gcataaaata atactgtttg actagcttgt accgcttgaa tatccttagt
      541 ataaagactg attaggaaag gccccgcaaa ataagtcaca ccagccacca aaaacataaa
      601 cagtaaagaa aggccaaagc ttgttcgaaa aatatgtcga accatagcct tatcctgtct
      661 gtgttttgct gtaaggatga ttgtagcggt agcaatccct aaagccggca tatagttaaa
      721 ctgcgtcagg gtttctccaa tcgcatttcc agctaccgtc gctgttccta agctggtaat
      781 caacgccacg actaccacat ctcccacccg catcatcaac cgttcaccag ttgctggcag
      841 agcaagtcta agcagttctt tatcaaagga ccaggtccat ggttttaaag aaattggtaa
      901 gcgagaccaa agcaggctac aaccaatcaa ccgcgataag accgtgccaa tcgcaactcc
      961 agctacacct gcatgaagca caaagactgc aaatgctgaa aatagtgcat ttagaacgtt
     1021 tgagagcagg ctgatataca taggaaaacg tggttccccc aaggttcgta agattgcact
     1081 caaacttgcc atgagtcccc aaaaaggtgc tcccccacca accaatatca aataaagacc
     1141 tccagcctgt accacttctg actctgcccc caagctagca agtactattg gacctgccag
     1201 aactgaaaaa attcctaaaa ggagaccgat aaaaactgta aacttgacac tttcactagc
     1261 agttcctcca atgccttctg aacccccttc acccaaagcc tgagccaaac gagaagacac
     1321 cgctgctgct agggcaataa aaaccgcctg atagacggct aaaatattat tagcaaggga
     1381 aactccagac aaggctacga tgccaatact ggctactaga taagagtcta ctacccccat
     1441 taacatctgg agaaggtttt cccccatggc tggaaaggca atggttaaaa tttctttata
     1501 ggtttgtttg ttcataatct ctatttcaac tgtttaatat ctactatttt atcacaaacc
     1561 agattcaata gctcttcatc gtgagaaatt aacaaaacca acttattctt catttttaag
     1621 tctactaaag cggaagcaac ttgctgcatt cgtctcaaat ccaaaccgct cgtcggttca
     1681 tcaaaaatga aaatttcctt atctgccagt aagctggcca ctatcatcac acgctgttgt
     1741 tcaccgcccg ataaactgac cggatgtctc tccaacaaat ccgatagacc aaaacgagct
     1801 accaaatgtt catcgacagc tctatttttc cttccaagag taagttctcg ctcaacactt
     1861 tccgcaaata gttggagtcg aacatcctgc ataacaaagg ctgttttttc caatctctgg
     1921 cgactagata aggtttgacc ttgccaagaa atcctactct tcttatcttc cgctaaaccg
     1981 accagataac gagcaaggct tgtttttcca gaaccattcg ctccaaccag actaattgaa
     2041 cccggcttcc caaccttggc aatcaagagc atatagactg gaccagctaa gagagctgct
     2101 ccagctggtg catacatcat attacccgaa cgatcaaaaa ccaagctcaa caaggttcct
     2161 agaccaacac aaacaaagta gagggcagca aaagcccctg ttaccatcag atcttttact
     2221 tgtaatttct tcatttattt ttcctttact aattgcccgt cttccaagta gaaaatggta
     2281 tccgcacaag ccatggtcga gcttctatga gaaatcaaga agaccatgcc ctgacagtgt
     2341 tccttaacca gttggataaa gcgggcttca ttgagcgagt ctaggttact agttggctca
     2401 tcaaagatat agcaatccgc atttttgagc agggcccgca tgagttccaa acgttgccct
     2461 tctcctgctg aaaaatcact ggcctccaca agcgtatcca agccctgcgg tgctgccaaa
     2521 atccgttctt tcatgtggca tttttctgcc aaatccatga tggtttcatc cgacacatcc
     2581 gtccgtccca agaggagatt ttcacgaatg gtctggcgga agatttgtgg cacctgtggt
     2641 acataggcaa aagccccctg caaatgagcc ttatctacct gccgactatc caagccagac
     2701 aggcggattt ggccttgctg ccaatcatac caccgcataa ttagtttcat caaggtggat
     2761 tttcctgcac cggactcacc gaccaagcca ataataccac cctgttcaaa ctggacagac
     2821 aggttccgat agagccctgt ttctcggtca tcgtagtcga agtccagttg ttcgatagca
     2881 atgtccgtca ctctgacctc aacaagctca cctgttcgct ctgcttcttt ttcatccaaa
     2941 agagcataga tattgcgacc agcattcata gcccgcttga aaccaagagg cagacggctg
     3001 agctccaaga agggagcaaa ggagctagtg aaagaaacca gcagggctac tcctgttgct
     3061 aagtccaaac tctgactacc caccaagtcg aaggtcagcc aggcaaagca cataatagat
     3121 agtcctacca ctaaaaagga aacagcatac tgcatgaagt tggtctgggc aacctttctc
     3181 tcctgaccat tgacctcttg gcttttctgt gtcagcttgg caaagcgagc atctgtctgc
     3241 tggaactgca tcaggtcctt catgctcttg agactttcaa taaagtagga cacatagccc
     3301 ttacggctgg cattttgctc cttgagcaag ggctctaact gctgagcaaa acgatttgga
     3361 atggcaatcg ccaacagagc ataggtcacc agagccagca aagccaaacc ccagctggat
     3421 agaccaaaat agactgtcaa accaaaagcc accaaaatcc ctgtacaaat tggggcaata
     3481 gtatgggcaa agaaaacctc caaggcctca atgtcctccc caatcatctt aagcaggcta
     3541 ccgctgtcct gccgatccaa tttcccagga gccagggccc gcagttttgc aaaaatcaaa
     3601 cgacggtaag ccgccagcgt atgaaaggcc acataatgac caaagtaatg ctcaccatag
     3661 cggaaggcac cacgtaaaag agctaggaga atgaggattc ccaaggtcca tagcggagct
     3721 ggattgccag caagggctgc aaaagccaga tagaccagcg ttactggaat agcaatggtc
     3781 accacctgtc ccaagaccgc aaagcaaacc gccaccgcaa tccgtggcaa gaggtgcttc
     3841 atggactgca agagtctcgg aatcagcacc cgtgtcagca tttctttctt atccataaac
     3901 ggcttcctcc aattctcttt gttgatcgac cagctgacca taagccaagc tagtctgata
     3961 aagcgtggca tgattgcctg tcacagctcc ttcagcagac aggaagagga tgtcatctgc
     4021 ctgctccact tgtttcatct tatgtgtaat aataatagca atcgcatcct tgaccaccaa
     4081 gtcaatgagg tcgtaaatca agccttcatt gtcttgatcg acgctggacg tcacctcgtc
     4141 aaagatataa agggagcgtt ttgccaaaac cgcacgggca cagataacct actggcgttg
     4201 accaggcgac aagaaagcac cgtcgtcacc aacaaccgta tccaaaccgt ctggcaaatt
     4261 cttgacaaac tgcaagaccc catgctgatc tgcccaagcc aagatgtcct ccttagacca
     4321 gtcacaagcc atgcgtaaat tttcatagat tgactggttg agcaaggttg actggcccga
     4381 cacatagagg acctgctcat tcagagataa ctgactgaca cctgaaatct cctactcacc
     4441 caagtaaatc gctcccttat ccgcccgcaa gcgtcccaag agcagctggg ccaaggtcgt
     4501 tttcccctgc cctgactcac ctgccaaagc atagacttta ccagccgtca tggtcataga
     4561 aatatctttt aagactggct tttctccgta gacaaaggcc agattttcca gtttcaaact
     4621 atcaaaaaca ggcacatgga aagaatcgat ttcctgctcc gctgtcatac tgtccaagaa
     4681 accaaaaatg cgatccgcca tcttggtatt catcatcacc aagtgcatac cgtagccctg
     4741 ctcccgtatg ggtgcaaaaa actccgtcgc aatgaggaca aagaagagca tactaaagag
     4801 ggacaaattg cctgctgcta ggctattgac cgccacaaaa ccagacaagc caatccccag
     4861 atacatgacc gaatccatgt agcctaccgc ttgcaactga aagctgagta gctccatggt
     4921 cgcatcgcgg aagtcctcgg cctgctcatt gaaggtcttt tcgtaggtgg catccgcctg
     4981 ataggaatag agggtgttga gccccttgag gtcgtccagg aaaagattgc ccacatccat
     5041 ataggaactc caatagcgat tcataatccg cttggaccgc ttctgcatag cgataatgga
     5101 aatgggaatc agaggcaaag ccaagatgaa aataaccgca cccagcggga aaaccagaaa
     5161 aaccagcaaa aaaacagttg cacagttgaa ctgggtccgc agagaagatg ccatgtagta
     5221 ggaatagtag gtatccaggc tatcaatccc ctgggaagcg atgttaaaga catccgctgc
     5281 tgtcgctttt gacgcaaact gaccgtcctt ggccaagagg gcctcaaaga aggactgctt
     5341 gagcgaatcc cgtgcaaact gggagcccaa gccctgcaaa cgcttggcaa tcaaggccac
     5401 accatatcca aaggcattga gcccgagcaa gatgaggaca aactgtacta aatcaacctg
     5461 cccgccctca tagtatgtca caaagccctt agcaatcagg taaaaggaaa taatccgcat
     5521 caaaaactgc acccaggata gaaaggctgc caaataaacc agcttcatct tgggtttgat
     5581 gcgttctttg agtcgcttca agagggct
//
LOCUS       DBNFEG010000140         5577 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.140, whole genome shotgun sequence.
ACCESSION   DBNFEG010000140 DBNFEG010000000
VERSION     DBNFEG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.140"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            37..957
                     /locus_tag="AC1PNG_001703"
     CDS             37..957
                     /locus_tag="AC1PNG_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter solute-binding protein,
                     Zn/Mn family"
                     /protein_id="HIP6702193.1"
                     /translation="MLKKILGACLSVLAGLCLMACSAQKEASQVQPGMKIVTSFYPIY
                     SLVKEVSGNKNDVRMIGSRQGIHSYEPSAADIKAIYDADVFIYHSRILESWAGRLEPN
                     LQGSSVKVLEASTNLPLTKVPGLEDMEAGQGIDEASLYDPHTWLDPVLVGQEAVAIGE
                     LLAESDPKNADYYRQNAATLEEKAQKLADKYSPIFLKAASKTFVTQHTAFSYTAQRFG
                     LKQLGIAGVSEEEPSPRQLAEIKEFVDTYKVQTIFTERGSSDKLAKALATSTGVDLKV
                     LDPLEADPENNLTYLENLEQVLETLAQELK"
     gene            979..4134
                     /locus_tag="AC1PNG_001704"
     CDS             979..4134
                     /locus_tag="AC1PNG_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HIP6702194.1"
                     /translation="MKKKAVVGSVAALVLGMSLCSYQLGRYQATEEQKNRVSYIEDSQ
                     KEESLVAEQLTPEQVSAKENIDAEQIVVKITDQGYVTSHGDHFHYYNGKVPFDAIFSE
                     ELVMKDPNYVLQDSHIINEVQDGYVIKVDGKYYLYLKDPSKHKNVRSKEEVERQKGIS
                     SADSKNQAAGNSKDGRYRTDDGYVFNPTDVIEDTGDGFIVPHGDHFHFIPKKDLSAAE
                     LKAAQDYWNQKGSVSSASGSQYGDRNNRAQQITISAGQGQDLASLLAQLDATPLSQRH
                     VEADGLVFDPRTITKKTAAGVIVPHGDHYHFIPYSQMSPLEEKISRMIGVNGAGVSSG
                     AQASHSQHTQTQPNRPVTPIGTVTTQPVSPTQPVLSTQPKQSTGKVVSYMGHQIPAYG
                     KGVDGKAYFTSDGYIFSKDSITSVDDQGLIASHGDHFHYVGFGELEDFEIKQVEEWVN
                     EKAGKQVPPKTSEQVGNDAKPTTPSQGNDSKPVKPIQEENRPTFEYKQVTAKRKLAGK
                     VVYEMEVGGKTYTYGRDELDLMKISFAELTLAEKDKQYIFDIAPLAEGDLKPAMLVGM
                     DQIPMKGANATYDTGQSFIIPHIDHIHVLPYTWLSKEQIATIRYIMQHPEIRPSAWTT
                     SGHGDGEATDLVPPILNATPKANRLGLKNWQIIHTAEEVMDARAKGKFATNDGYIFSA
                     EDVLDPASFVFSQAFSLPRATGGSLRSISKKDLSKEELEAVQTLLDKRDAEELAQNVT
                     PIEKRAGLKNWQIVHSAEELAEAKAAGKYTTKDGYIFDPADLLDPKVKIGTDNYRIPR
                     VITDGYRRINKSDLNYLSELIPAEAMVAQREKSNSSSPSTPAPTETGASAGETTKPEQ
                     PQVAKETAEEIYNRVEAKKVVPFEALTYNAGYATEVRNGTLVIPHQDHYHYVSFKWFD
                     QGSARSPEGYSLEDFLATVKYYMTNPQERPVSDDGWGVFTPNTPSESTEETETEESDE
                     EIISEETEEIDEFTEELKRRAEEFGMDFKTFEQSLVTLSDRYKVSFEAFEYDAASKVV
                     RLVDKDGVKRTISLPSLEEQV"
     gene            4215..5566
                     /locus_tag="AC1PNG_001705"
     CDS             join(4215..4671,4671..5566)
                     /locus_tag="AC1PNG_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIP6702195.1"
                     /translation="MKLSYEDKIKIYELRQSGQSIKNLSNQFTISESVIQYMLRLIDR
                     YGINIVKKGKNTYSPPELKQEMIDKVLLQKQSSLSVSLDYALPNRGTLPNWIAQYKKN
                     GYTILEKTRGRPPKMGRKPKKTWEEMTELERLQEENERLRTENVYLKKLRGASFKGRS
                     QRTRKAQTVREMVAEGFRLDVLLTIAQLVPSTYHYQVKQLDKPDKDKDLKAEIQAIYD
                     ENKGNYGYRRIHLELRNRGFSVNHKKVQRLMKELGLTARIRRRKRYKSYKGDVGKKAP
                     NLIERAFEGAKPFEKCYTDVTEFALPNCAEKLYLSPVLDGYNSEIIDFTLSRSPNLIQ
                     VKTMLEKVFPEETYPNTILHSDQGWQYQHEVYHRFLASKGIRPSMSRKGTSTDNSMME
                     SFFGVLKTEMFYGFEKNFKSLDQLEQAITEYIFYYNNKRIKTKLKGLSPVQYRTKSFQ
                     "
BASE COUNT         1861 a         1068 c         1254 g         1394 t
ORIGIN      
        1 ttaactggtt aatgtttttt gtgaaaagga ggaaacatgc taaagaaaat acttggagct
       61 tgtctttcgg ttctagctgg tttgtgcttg atggcttgct cggctcagaa ggaagcgagt
      121 caagtgcagc caggaatgaa aatagtcacc tcattttacc cgatttattc actggttaag
      181 gaagtgtcag ggaataaaaa tgatgttcga atgattggct caagacaggg catacactct
      241 tatgaaccat cggctgcgga cataaaggcc atatacgatg cagatgtttt tatctatcat
      301 tcgcgaattt tggaatcctg ggcaggacgt ttggaaccta atttgcaagg ttcatctgtc
      361 aaggttttgg aggcttcgac aaatttaccg ctgacaaagg ttccagggtt agaagacatg
      421 gaagctggtc aagggattga tgaagctagt ttgtatgacc cccacacatg gctggatcca
      481 gttttggttg gtcaggaagc tgttgcgatt ggtgaacttt tagcagaaag tgatcctaag
      541 aatgcggatt attatcgtca aaatgccgca actttagagg aaaaggcgca aaagttggca
      601 gacaagtata gcccaatctt tttaaaagcc gcatcaaaaa catttgtcac tcaacacaca
      661 gccttctctt acactgcaca acgcttcggg ttgaagcagt tagggatcgc aggagtttca
      721 gaggaagagc caagcccaag gcagttggca gagattaaag agtttgtcga cacttacaag
      781 gtacaaacaa tttttactga gcggggttcc tcagataagt tagccaaggc tctggcaact
      841 tcgacaggtg tagacttgaa ggtcttggat cctctggagg cagatccaga aaataacttg
      901 acctatttag aaaacttaga gcaggtttta gagacacttg ctcaagaatt gaaataataa
      961 tcacataaag gagagaagat gaaaaagaaa gcagtcgttg gttcggtagc agcccttgtg
     1021 ttggggatga gtttatgtag ctaccagttg ggtcgttatc aggcgacaga agaacaaaaa
     1081 aatcgagtgt cctatattga agatagtcag aaggaagagt cactggtcgc ggagcaatta
     1141 actcctgaac aggtttcggc aaaggaaaat attgatgctg agcagattgt tgtcaaaatt
     1201 actgaccaag gctatgtgac ctctcacgga gatcatttcc attattacaa tggcaaggtg
     1261 cctttcgatg ccatctttag tgaagagttg gtcatgaagg acccaaacta tgtcttacaa
     1321 gacagtcaca tcatcaatga agtccaggat ggctacgtca taaaagttga tgggaaatac
     1381 tatctctatc taaaagatcc tagcaaacat aaaaatgttc gtagcaaaga agaagtggag
     1441 cggcaaaaag gaatctcttc tgcggacagt aagaatcagg cagcaggaaa tagtaaagat
     1501 ggccgctaca gaacagacga tggctatgtc tttaatccga cagatgttat tgaagacacg
     1561 ggagatggtt ttatcgttcc acacggcgac catttccact ttattcctaa aaaagattta
     1621 tcagctgccg aattgaaggc tgctcaagat tactggaatc aaaaaggaag tgtgagttcg
     1681 gctagtggta gtcaatatgg cgatagaaat aatagagctc aacagataac aattagtgcg
     1741 gggcaaggtc aggatttggc tagtttattg gctcaattgg atgcaacacc cctatctcaa
     1801 cgccatgtag aagcggatgg attggtattt gacccaagaa cgattacgaa gaaaaccgca
     1861 gcaggtgtca tcgttccaca cggcgatcat taccacttta ttccatacag ccagatgtct
     1921 cctctagaag agaagatttc tcgaatgatt ggtgtaaatg gagcaggtgt ttcttctggc
     1981 gcacaagcaa gtcactccca acatacacaa acacaaccaa atcgtccagt aacaccaatc
     2041 ggcacggtta caacgcaacc ggtatcgcct acacaaccag tattatcaac acaaccgaag
     2101 cagtcaacag ggaaagtggt atcctatatg ggacatcaaa ttcctgctta cggaaaaggg
     2161 gtagatggta aggcatattt tacaagtgat ggatacatct ttagcaaaga ctctattacc
     2221 tcagttgatg atcaagggct gatagctagt catggagatc atttccatta tgttggtttt
     2281 ggcgaactcg aagattttga aatcaagcaa gtagaggaat gggttaatga gaaggcgggc
     2341 aaacaagtac caccgaaaac atctgaacaa gtaggaaacg atgctaaacc cacaacacca
     2401 agtcaaggga atgattcaaa acctgtaaaa ccaattcaag aagaaaatcg ccctacattt
     2461 gaatacaaac aagtaacggc taaacgtaaa ttggctggaa aagttgttta tgaaatggaa
     2521 gtaggtggaa aaacttatac atatggtcgt gacgaactag atttgatgaa gatctctttt
     2581 gctgagttga ccttggcaga aaaagataag cagtatatct ttgatattgc accgcttgca
     2641 gaaggagatc ttaagccagc catgttggtc ggtatggatc aaattccgat gaagggagcg
     2701 aatgcaacct atgatactgg acaatccttt attattccac acattgatca catccacgtc
     2761 ctaccttaca catggttgag taaggaacaa attgcaacga ttcgctatat catgcaacat
     2821 cctgaaattc gtccatctgc ttggactaca agtggacatg gtgatggaga agcaacggat
     2881 cttgttccac caattctgaa tgcaacacct aaagcgaacc gcttaggctt gaaaaattgg
     2941 caaatcattc acactgcaga agaagtcatg gatgcacgtg caaaagggaa atttgcgaca
     3001 aatgatggct atatcttctc ggcggaagac gtactagatc cagcaagttt tgtctttagt
     3061 caagcgttca gtctaccaag agcgacaggt ggttctctgc gttcaatttc caagaaagat
     3121 ttatctaaag aagaattgga agctgtacaa actcttcttg ataaacgaga cgctgaagaa
     3181 ttggcgcaaa atgtcacacc gattgagaaa cgtgcaggtt tgaaaaattg gcaaattgtt
     3241 cactcggcag aagagttggc agaagctaag gcagcaggaa aatatacaac gaaagatggc
     3301 tatatttttg acccagcaga tctattagat cccaaagtga aaataggtac ggataactat
     3361 cgcatcccac gtgtgattac agatggttat cgtcggatta acaagagtga tttgaattac
     3421 ttaagtgagc taatcccagc agaggctatg gtggctcaac gtgagaaatc taattctagt
     3481 tcaccgtcaa cacctgcacc aactgaaaca ggagcaagtg caggcgagac aacaaagcca
     3541 gaacagccac aagttgccaa agaaacagca gaggaaattt ataaccgagt ggaagcgaag
     3601 aaagtcgttc ctttcgaagc actgacctac aatgctggct atgcgaccga agtacgtaac
     3661 gggactctgg taattccgca tcaagatcac tatcactatg tatcctttaa atggtttgac
     3721 caaggtagtg caagaagtcc tgaagggtat agtttagaag atttcctagc aacggttaag
     3781 tactacatga ctaatccaca agaacgccct gtttcagacg atggctgggg agtatttaca
     3841 ccaaatacac catctgaatc aacagaagaa acagagacgg aagaaagtga tgaagaaatt
     3901 atttctgagg aaaccgagga aattgacgag tttacagaag aattaaaacg aagagcagaa
     3961 gagtttggca tggacttcaa aacctttgaa caaagtctgg tcacactctc agaccgttac
     4021 aaagtttctt ttgaagcttt cgaatatgat gcagcaagca aagttgttcg acttgtagat
     4081 aaggatgggg tcaaacggac catctccctt ccaagtttag aagaacaagt atagtaaaca
     4141 aaatacctgt agcgctgatg aactgcaccc caaaagttag acagaaaaaa tctaacattt
     4201 ggggtgctat ttttatgaaa ttgagctatg aagacaaaat caaaatctat gaactacgtc
     4261 aaagtggtca gtctatcaag aacttgtcaa accaatttac tatttcagaa tctgttatcc
     4321 aatatatgct tcgattgatt gataggtatg gaataaacat tgtcaagaaa ggtaagaaca
     4381 cttattctcc tccagaactc aagcaggaaa tgattgataa agtacttctt caaaagcagt
     4441 cgagtctgtc agtctcactt gattacgcct tgccaaaccg aggaactctc ccaaactgga
     4501 tagcacaata caagaaaaac gggtatacta ttcttgagaa aacaagaggg aggccaccaa
     4561 agatggggcg taaaccaaag aaaacctggg aagagatgac ggagttagag cgacttcagg
     4621 aggaaaatga acggcttaga acggagaatg tctatctaaa aaagttgaga gcgcttcgtt
     4681 taagggacga agccagagaa caagaaaggc tcaaacagtt agagagatgg tcgcagaagg
     4741 atttcgacta gatgtactac tgacgattgc tcaattagtt ccttctacct atcattatca
     4801 agtcaaacaa ctggacaaac cagataaaga taaggactta aaagctgaaa ttcaagccat
     4861 ttatgatgaa aataagggaa attatggcta ccgtcgcatt caccttgagc taagaaatcg
     4921 tggattttct gtcaaccaca agaaagtaca acggctgatg aaagagttag gtttgactgc
     4981 tcgtattcgt agacgcaaga gatacaagtc ttacaagggt gatgtgggca agaaagctcc
     5041 taatctcatt gagagagcct ttgaaggggc taagcctttt gagaagtgct atacagatgt
     5101 gactgagttc gctctgccaa attgtgctga aaaactctat ctttctccag ttctggatgg
     5161 ctataatagc gaaattattg attttacctt atcccgctct ccaaacctca tacaagtcaa
     5221 gaccatgctt gagaaggtct ttcctgagga aacttaccca aataccattc tccatagtga
     5281 ccaaggttgg caataccaac atgaagtcta tcaccgtttt cttgcaagta aaggcatacg
     5341 tccttccatg tctcgcaagg gaaccagcac agataatagc atgatggaat ccttctttgg
     5401 cgtcttgaaa acagaaatgt tttacggatt tgagaaaaat ttcaagtctc tggatcaact
     5461 ggagcaagct atcaccgagt atatttttta ctacaacaat aaacgcatta aaacaaaact
     5521 aaaaggactt agtcctgttc agtacagaac taaatccttt caataatatt ttttgtc
//
LOCUS       DBNFEG010000141         5557 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.141, whole genome shotgun sequence.
ACCESSION   DBNFEG010000141 DBNFEG010000000
VERSION     DBNFEG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.141"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(453..1388)
                     /locus_tag="AC1PNG_001706"
     CDS             complement(453..1388)
                     /locus_tag="AC1PNG_001706"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HIP6702196.1"
                     /translation="MSKFLVFGHQNPDTDAIASSYGWAHLEREVFGRDAEAVALGTPN
                     EETAFALDYFGVTAPRVVESAKAEGVSQVILTDHNEFQQSIADIKEVEVAAVIDHHRV
                     ANFETANPLYMRLEPVGSASSIVYRAFKENGVTPPKEVAGLLLSGLISDTLLLKSPTT
                     HTTDPQVAAELAEIAGVNLEEYGLTLLKAGTNLASKSAEELIDIDAKTFGLNGNDVRV
                     AQVNTVDIAEVLERQAEIEATMTAASAANGYSDFVLMITDIVNSNSEILALGSNMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTDAFNA"
     gene            1526..3223
                     /locus_tag="AC1PNG_001707"
     CDS             1526..3223
                     /locus_tag="AC1PNG_001707"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIP6702197.1"
                     /translation="MKFSDYTYVRPDYETIKAQFSDLTDQLAQASDLETTRTLVAAVT
                     KLLNLVDTQYNLWMIRHTIDMNDEFYNEETKFWNEYSPLFEELTTNYYRVIVQTPYKA
                     ELADILPETFFMQAENKLKTFSSDVIPLFQKENELTDEYSKLIAGAEIDFQGQTYNLA
                     QMGPFGQSTDREVRKAASAATTAFFESKEADFDRVYDELVKVRTEIAHKLGFKDYVEY
                     GYLKMNRFDYNCDMVKVYREEILKHIVPIVQNLRERQAKRLQVPSLKHYDLNLEFLDG
                     NAVPQGDPDFILSQAQDMYRELSAETGEFFDFMIEHELLDLVAKPGKNSGGYCTYIPD
                     FKSPFIFSNFNGTSGDIDVLTHEAGHAFQVYRSRWIQSPEVVWPTYETCEIHSMSMEF
                     MTWPWMDRFFKEQVDKYKFTHLASALLFLPYGVLVDHFQHEVYEHPEMTPAERKATWK
                     KLQDLYLPDRDYSESEALNRGIFWYRQGHIFASPFYYIDYTLAQVCALQFWKRTQVDH
                     DENAWEDYIRICDLGGTKSFLQVVEAANLQSPFKEGALESTAKAAADWLNAVKDSDL"
     gene            complement(3332..4123)
                     /gene="pflA"
                     /locus_tag="AC1PNG_001708"
     CDS             complement(3332..4123)
                     /gene="pflA"
                     /locus_tag="AC1PNG_001708"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HIP6702198.1"
                     /translation="MMTEIDYRKVTGLVHSTESFGSVDGPGVRFVVFMQGCHMRCQYC
                     HNPDTWDLVNPAATERTAEDVLNEALRFRMFWGKEGGITVSGGEATIQIDFLIALFTL
                     AKEKGIHTTLDTCALTFRKTERYLEKYSRLMEVTDLVLLDIKEMNPEQHRIVTGHSNK
                     TILECAQYLSDIGKPVWIRHVLVPNLTDRDEDLIELGKFVKTLKNVERFEVLPYHNLG
                     EFKWRELGRPYPLEGTKPPTKARVENAKALMETETYQEYLNRIKG"
     gene            complement(4244..>5557)
                     /locus_tag="AC1PNG_001709"
     CDS             complement(4244..>5557)
                     /locus_tag="AC1PNG_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIP6702199.1"
                     /translation="IYLQLLILFLLTLFNAFFSASEMALVSLNRSRVEQKAADGERKY
                     IRLLQVLENPNNFLSTIQVGITFISILSGASLANDLGAVFAKWMGNSTTAQTAGYWLA
                     LAMLTFVSIVLGELYPKRIAMNMKENLAVVTAPVIIFLGKIVSPFVWLLSAATNLISR
                     ITPMNFDDADEQMTRDEIEYILTKSEKTLDAEEIEMLQGVFSLDELMAREVMVPRTDA
                     FMVDIEDDTATIMAAILKQNFSRIPVYDGDKDNIIGLIHTKKILSEAFSNGFDNLNIR
                     RIMQEPLFVPETIFVDDLLTSLRNTQNQMAILLDEYGGVAGLVTLEDLLEEIVGEIDD
                     ETDKTEIFVREIADNTYIVQGNMTLNDFNEHFEMELESDNVDTIAGYYLTGVGTIPSQ
                     DEKVSFEVDSKDHHLVLINDKVKNGRVTKLKLLITPLEKEETDKD"
BASE COUNT         1618 a         1287 c         1070 g         1582 t
ORIGIN      
        1 actttgctgg ttctcatcgt catttagttt ttcttttatt acttctgtaa tcaatttgaa
       61 ttgacagaac gattagaagc actttacttg taaacaaaat ttctaaatta tggcacattc
      121 aaagtaaaaa actagcgcct gaagttttct atgatacaga tacttgttta tctcggagtg
      181 ctcctttaat tcgtcaactt atactcaatg aaaatcaaaa tcaaactagg cgacgcagat
      241 acagatagaa ctgaagttca tcaaatcaag ttaacaacgt ctgattttcg aagagtatta
      301 aataagagtg ggtttatttg tcatgatgcc acttgtttat tttgttaact atcacttcat
      361 aaaacatgat tgtcctcata ataaaaagca cccgaaggtg ctagataagg ccttcctacc
      421 gacttcacgt tcgaggtcgg tagaaagctt cattaggcgt tgaaagcatc tgtcaattgt
      481 ggcaccactt gtttcttacg ggaaacggca cctgcaagga aagcgtggtt gttttccaac
      541 ttgaagttga aggccgcttc caccttgtcc atgttgcttc ctagggcaag gatttcagag
      601 tttgagttga cgatgtccgt aatcatgagg acaaagtcag agtagccatt tgctgcggaa
      661 gctgctgtca tagtggcttc aatctctgct tggcgttcca aaacttccgc aatatcaaca
      721 gtgttgacct gagctacacg gacgtcattg ccattcaaac caaatgtttt ggcatcaatg
      781 tcaatcaatt cttctgctga cttgctggca aggtttgtac cagctttcaa gagtgtcaaa
      841 ccgtattctt ccaagttcac accagcaatt tcagccaatt cagctgctac ttgtggatct
      901 gttgtatgag tggttggcga tttgagcaag agagtatctg aaatcaaacc tgataggaga
      961 agtccagcta cttcttttgg aggtgttact ccgttttctt taaaggcccg gtaaacgatt
     1021 gatgatgctg aacctactgg ttccaaacgc atgtacaatg gatttgccgt ttcaaagttt
     1081 gcgacacggt ggtggtcaat aacagctgcc acttccactt ccttgatgtc tgcgattgat
     1141 tgttggaatt cattgtggtc tgtcaagatg acttggctaa cgccttctgc ttttgcagac
     1201 tcaaccacgc gcggtgcagt aacaccaaaa tagtcaagcg caaacgctgt ttcttcattt
     1261 ggcgttccaa gtgcaactgc ttccgcatca cgaccaaaca cttcacgctc caagtgagcc
     1321 cagccgtacg atgatgcaat ggcatctgta tcaggatttt ggtgaccaaa aactaaaaat
     1381 ttagacatgt ctacaacctc atttttctaa taatctccta cattttacca taaatagaaa
     1441 gacttgtgaa catggacggg tcgggatttg ccaaacgttt tcaaaaagag taagatagaa
     1501 ggtagcaata caaaaggaga aacatatgaa attttcagac tatacctatg tccgtccaga
     1561 ttatgaaact atcaaggctc aattttctga cctgacagac cagttggctc aggcaagtga
     1621 cttggaaaca actcgcacac ttgtggcagc cgttaccaaa ttgctcaact tggttgatac
     1681 ccagtacaac ctctggatga tccgtcatac tatcgacatg aacgatgagt tctacaatga
     1741 agaaaccaag ttttggaatg aatattcgcc actttttgaa gaattgacca ccaactacta
     1801 ccgtgtcatt gtccagacac cttacaaggc tgagcttgca gacattttac cagaaacctt
     1861 cttcatgcag gctgagaaca agctcaagac cttctcatct gatgttatcc ccttgttcca
     1921 aaaggaaaat gaattgaccg atgagtacag caaactgatt gctggagcag agattgattt
     1981 ccaaggtcag acctacaacc tggcacaaat gggaccattc ggtcaatcaa ccgaccgcga
     2041 ggtccgcaaa gctgcttctg ctgccacaac tgcattcttt gaaagcaaag aagctgactt
     2101 tgaccgtgtt tacgatgaat tggtcaaggt ccgcacggaa atcgcccaca aacttggctt
     2161 caaggactat gtggaatacg gctacctcaa gatgaaccgt ttcgattaca attgtgacat
     2221 ggtcaaggtt taccgtgaag aaatcctcaa gcatatcgta ccgattgtgc aaaatcttcg
     2281 tgaacgccaa gccaaacgct tgcaggtacc aagcctgaaa cactacgacc tcaatcttga
     2341 gttcttagac ggaaacgctg tgccacaagg tgacccagac tttatcctca gccaggccca
     2401 agacatgtac cgtgaattgt ccgcagaaac aggcgagttc ttcgatttca tgattgagca
     2461 tgaactcttg gacttggttg caaaaccagg taaaaacagc ggtggctact gtacctatat
     2521 tcctgacttc aagagcccat tcatcttctc caacttcaac ggaaccagcg gtgatattga
     2581 tgtcttgacc cacgaagcag gtcacgcctt ccaagtctat cgttcacgtt ggattcaaag
     2641 tccagaagtt gtttggccaa cctacgaaac ctgtgaaatc cactccatgt ctatggaatt
     2701 tatgacgtgg ccatggatgg accgtttctt caaggaacag gtggataagt acaagtttac
     2761 ccacttggca agtgcccttc tcttcttgcc ttacggtgtc ttggtggatc acttccagca
     2821 cgaagtctat gaacatccag agatgacacc agctgaacgc aaggcgactt ggaaaaaact
     2881 ccaagacctc tacttgccag accgtgatta ttctgagtca gaagccctca accgcggcat
     2941 cttctggtat cgtcaaggtc atatctttgc tagtccattc tactacatcg actatacctt
     3001 ggctcaagtt tgtgcccttc aattctggaa acgtacacag gtagatcacg atgaaaatgc
     3061 ttgggaagac tatatccgca tctgtgattt gggcggcacc aagtccttct tgcaagtggt
     3121 tgaagccgca aacctccaat caccgttcaa agaaggtgcc cttgaaagca cagccaaggc
     3181 tgctgccgat tggttaaatg cagttaagga tagcgactta taaaacacaa aaatctgaat
     3241 gaatcaaatg actcattcag attttttgat tatatgatac ttgtccctaa cacattaaat
     3301 ctttttcgca gcaatctcag acctctcact attatccctt aattctattc aagtactcct
     3361 gataggtctc tgtctccatc aaggcctttg cattttccac tcgagccttc gttgggggct
     3421 tggtcccctc tagtggatag ggtctgccca actcacgcca cttaaattca ccaagattgt
     3481 gataaggtaa cacctcaaaa cgttcgacat tttttaatgt tttaacaaac ttaccaagtt
     3541 caatcaagtc ctcatctcgg tcagtcaaat tcggcaccag gacatgacga atccaaacag
     3601 gcttaccaat gtctgataga tattgagcac attctaaaat agtcttgtta ctatgacctg
     3661 tcacaatgcg gtgttgctca ggattcattt ccttaatatc aaggagcacc aaatctgtta
     3721 cttccataag ccgactatat ttttccaagt aacgctctgt cttacggaaa gtcaacgcac
     3781 aggtatccaa agtagtgtga atcccttttt cttttgcaag ggtaaacaga gcaattaaaa
     3841 aatcaatctg gatggttgct tcaccacctg aaacggtaat acccccttct tttccccaaa
     3901 acatacgaaa acgcagagct tcattcaaga catcttctgc cgttctctca gtcgctgctg
     3961 gattgaccag atcccaagta tctgggttat ggcaatactg gcaacgcatg tgacagccct
     4021 gcataaaaac tacaaaacgt acccctgggc catcaaccga accaaaactc tctgttgaat
     4081 gaaccaaacc tgtcacttta cgataatcaa tttctgtcat catctcatcc tcatcatttt
     4141 ataagactag tatatcacaa aaatgaaaac gcttctatca tttactttct ttctttcata
     4201 accatcatct tccgcaagaa aaaagctaaa agactaggtt gccctagtcc ttatcggttt
     4261 cttctttctc cagtggtgta atcaagagtt ttaattttgt tacgcgacca ttttttacct
     4321 tatcgttaat aagaactagg tggtgatctt tactatctac ttcaaaggaa actttctcat
     4381 cttggctcgg aatggtaccg actcctgtca gataataacc agcaatcgta tcaacgttat
     4441 cactttccaa ctccatctca aaatgctcat tgaagtcatt caaggtcata tttccttgga
     4501 cgatataggt gttgtcagca atttctcgaa caaaaatttc tgtcttatcg gtctcatcat
     4561 cgatttcccc aacaatttct tccagcaaat cttccaaggt aacgagacct gcaactccac
     4621 catactcatc taacaagatg gccatttgat tttgggtatt tctcaatgat gtcaacaaat
     4681 catccacgaa aatcgtttct ggaacgaaga gaggctcctg cataatgcgg cggatgttga
     4741 gattgtcaaa gccattactg aaagcttctg acaagatttt cttggtatga atcagaccaa
     4801 tgatattgtc cttatcgcca tcataaacag ggatacgtga aaaattctgc ttgaggatag
     4861 ctgccatgat ggttgctgta tcatcctcaa tatccaccat gaaagcatcc gtacggggaa
     4921 ccattacttc acgtgccatc agctcatcta aagagaacac accttgaagc atttcgattt
     4981 cttcggcatc taaggttttc tcactctttg tcaaaatata ttcaatttca tcccgtgtca
     5041 tttgctcatc cgcatcatca aaattcatag gagtaatacg gctgataaga ttcgttgcag
     5101 ctgataataa ccacacgaac ggactaacaa tttttccaag gaaaataatc actggtgcag
     5161 tgactactgc cagattttcc ttcatattca tggcaatccg cttagggtac aattcgccaa
     5221 gaacgattga aacgaaggtc aacatagcta gtgcaagcca gtatcctgcg gtttgcgctg
     5281 ttgttgaatt ccccatccat ttggcaaata cagctcccaa atcattagcc aagctggcac
     5341 ctgaaaggat ggagatgaag gtaataccga cctgaatggt tgatagaaaa ttgttaggat
     5401 tctctaaaac ttgaagcaag cgaatatatt tcctctcacc gtctgcagcc ttttgttcta
     5461 ctcgagaacg attgagggat acaagggcca tttctgaggc tgaaaagaag gcattgaaca
     5521 gagtcaataa gaataaaatt aacagttgta aatagat
//
LOCUS       DBNFEG010000142         5546 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.142, whole genome shotgun sequence.
ACCESSION   DBNFEG010000142 DBNFEG010000000
VERSION     DBNFEG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.142"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(97..240)
                     /locus_tag="AC1PNG_001710"
     CDS             complement(97..240)
                     /locus_tag="AC1PNG_001710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702200.1"
                     /translation="MINPMHLWFYSFKVYVLAALSGYILHYVTTPASVNDFKEVAALL
                     ERC"
     gene            complement(242..2479)
                     /locus_tag="AC1PNG_001711"
     CDS             complement(242..2479)
                     /locus_tag="AC1PNG_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HIP6702201.1"
                     /translation="MSRLSKLPCQPVHLAVLAVAAYFAVHSFSLLTMGLFSLLLAVFG
                     VRQGKTVFLKTLPLLALCGLFFGYQKVQWERADQSATEQVTTVQVIPDTIDINGDSLS
                     FRGRADGQTYQVFYKLTSQEEQTYFQKLTGLVQLEVEAEISLPAGQRNFKGFDYQAYL
                     KTQGIYRTVKITAIKKIVPVQSWNVFDWLSNWRRQALVYVKTNFPAPMSHYMTGLLFG
                     DLDSDFDQMSDLYSSLGIIHLFALSGMQVGFVIDKFRWILLRLGLTKETVDKLQIPFS
                     LVYASLTGFSVSVVRSLVQKILGNLGLRKLDNFAVTVFVCLLILPRFLLTAGGVLTFT
                     YAFLLTVFDFEDLGQVKKAAVESLSISLGILPVLMTYFYAFQPLSILLTFAFSFVFDV
                     LLLPGLSVIFLLSPIVKITWVNGFFVFMEKIIVWVADLGLRPWILGKPSELVLLLLLV
                     SLFLLYDFHRRKKWLLGLSLVLALLFFITKHPLENEVTVVDIGQGDSIFLRDMRGRTV
                     LIDVGGRVDFAAKEAWQERPRQANAERTLIPYLHSRGVDRIDSLVLTHTDTDHVGDVL
                     EVAKQVQIGRIYVSPGSLTVPDFVATLKEINVPVHVVEVGERLPIFDSYLEVLYPDGT
                     GDGGNNDSIVLYGRLLKTNFLFTGDLEQGELDLIESYPNLPVDVLKAGHHGSKGSSYP
                     EFLDHIEAKIALVSAGENNRYKHPHQETLERFDSRNIQVYRTDQQGAIRFRGWREWRI
                     ETVRR"
     gene            complement(2463..3125)
                     /locus_tag="AC1PNG_001712"
     CDS             complement(2463..3125)
                     /locus_tag="AC1PNG_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIP6702202.1"
                     /translation="MDTIKTYIELLKEYKWQIALPAVAGLLMATFLIFSQPAKSDQTG
                     LTDFPQTEQTSSGQEQTEEISTEVSKEPSQLVVDVKGAVDKPGLYTLEAGARVNDAVE
                     AAGGLTSQADPKSINLAQKLSDEAVVYVASKDENISVVTSTTASSAMSPEEKSTSLVN
                     LNTATEADLQTISGIGAKRATDIIAYREANGGFKSVDDLNNVSGIGDKTMESIRPYVT
                     VE"
     gene            complement(3193..3939)
                     /locus_tag="AC1PNG_001713"
     CDS             complement(3193..3939)
                     /locus_tag="AC1PNG_001713"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIP6702203.1"
                     /translation="MFYSYLRTLLTFLLWAINGNIHYHDREKILPQEENYILIAPHKT
                     FWDPVFLGYAAAPKQFIFMAKKELFKDRGFGWWISKCGAFPIDRENPGMAAIKYPVNM
                     LKKSDRSLVMFPSGSRHSSELKGGVAVIAKSAKVKLMPATYVGPMTIKGLLAGERIDV
                     AFGNPIDVSDIKRMDDTGTAEVTSRIEAEFKRLDDHAASFQTKKKPNILTYIYRIPVL
                     LLVALILGLTYAFSYIASFFWQPSTQLDKK"
     gene            4085..5530
                     /locus_tag="AC1PNG_001714"
     CDS             4085..5530
                     /locus_tag="AC1PNG_001714"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013990079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HIP6702204.1"
                     /translation="MITIERVLEILKADANFRHIKQNDQTDSTWTDVQFDALSYDSRT
                     VSPTTLFFAKGLAFKKEFLEKAIEAGLAFYVSEIDYEVDIPAIIVHDIKQAMSLIAME
                     FHGHPQKQLKLLAFTGTKGKTTAAYFAFNILKQSHKPAMLSTMNTTLDGKTFFKSTLT
                     TPESLDLFAMMAEAVKNGMSHLIMEVSSQAYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPTFEDYFYHKRLLMDNSRAVIVNAGMDHFEVVKEQVSSKDHDFYGPTSENQISQSAG
                     FDFTATGKLAGHYDIQLIGGFNQENAIAAGLACLRLGASLEDIHTGIAQTNVPGRMEV
                     LTQQNGAKVFVDYAHNGDSVKKLIDVVLEHQTGKVFLILGAPGNKGESRRKDFGLLLN
                     DYPQIEVILTADDPNREDPAAIAEQIRAHMTRPSNFILDREEAIRTAMSQTSSTKDAV
                     IIAGKGADAYQIVNGEKAAYDGDLEVAKQYL"
BASE COUNT         1586 a         1405 c         1217 g         1338 t
ORIGIN      
        1 atcaattctt aaattcaaaa tatagactat ttgaaataca atggaatgag caagactgct
       61 gactgagata gcccaatctg ctacaatcac agattgttag catctttcca ataaggcagc
      121 aacctctttg aagtcgttaa cagaagctgg cgtagtaaca tagtgcagaa tatagccaga
      181 taaggcagcg agcacataaa ctttgaaact atagaaccat aagtgcatag gatttatcat
      241 tttacctcct caccgtttca atcctccact ccctccaacc tcggaagcga atggctcctt
      301 gctggtctgt acgatagact tggatattgc gactatcaaa tcgttccaag gtttcttggt
      361 gaggatgctt gtagcggttg ttttctccag cagataccaa ggcaatcttg gcttcgatat
      421 ggtccaaaaa ttcaggatag gaagaacctt tggagccgtg gtgaccggct ttgaggacat
      481 cgacaggcag gttaggataa gattctatca agtccaactc cccctgctcc aagtcccctg
      541 taaagagaaa attagttttc agcaagcgac cataaaggac aatggagtca ttattgccac
      601 cgtctcctgt cccatctgga taaaggactt ccaggtaaga gtcaaaaatt ggtaagcgtt
      661 cgcccacctc aacaacatga acagggacat taatttcttt caaggtggct acaaagtcag
      721 gcaccgtcag gcttcccggc gacacgtaaa tccgaccaat ctggacctgc ttcgccactt
      781 ccagcacatc ccccacatgg tctgtgtcgg tgtgggtcag gactaaacta tcaatcctat
      841 ccacaccccg actatgcaga tagggaatca aagtccgctc tgcatttgcc tgccgaggcc
      901 gctcctgcca cgcttccttg gccgcaaaat caactcgtcc gcccacatca atcaggaccg
      961 tccgcccccg catatcccgc aaaaagatgc tgtccccctg cccgatgtcc accaccgtca
     1021 cctcgttttc cagcgggtgt ttggttatga aaaatagcag agcgaggacc aagctcagtc
     1081 ctagaagcca tttctttctc ctgtggaaat cgtagagtaa gaagaggctg accagcaaga
     1141 gcaaaaggac cagctcagac ggcttgccca gtatccaagg tcgcaatccc aaatccgcca
     1201 cccagacaat gattttttcc ataaagacaa aaaatccgtt gacccaagta atcttgacga
     1261 ttggcgacag gagaaaaatg actgacaagc ctggcaagag taggacatca aagacaaaag
     1321 aaaaggcaaa ggtcaagagg atagacaggg gctgaaaggc atagaaatag gtcatgagca
     1381 ctggcaaaat cccaagtgaa atactgagac tttccaccgc agcctttttg acctgcccca
     1441 agtcctcaaa atcaaaaacc gtcagcaaaa aagcataggt aaaggtcagg acaccaccag
     1501 ctgtcagcag aaatcggggc agtatcaaca gacagacaaa aactgtcacc gcaaagttat
     1561 ccaacttccg caagcccaga ttacccaaaa ttttttgaac caaagaccgc accaccgata
     1621 cggaaaaacc tgtcaagctc gcatagacaa gggaaaaggg aatttgtaat ttatcaaccg
     1681 tttctttggt caagcccaaa cgcagtaaaa tccagcgaaa cttgtcgatg acaaagccca
     1741 cctgcatacc agacagggca aagagatgaa tgatacccaa actggaatag aggtcactca
     1801 tctggtcaaa atcactatcc aagtccccaa acaagagccc tgtcatgtag tggctcatgg
     1861 gagccggaaa attggttttg acataaacca aagcctgcct ccgccagttt gacaaccagt
     1921 caaagacatt ccaagactgg actgggacaa tcttcttgat tgccgtaatc ttgaccgtcc
     1981 gataaatacc ctgtgttttc aggtaggcct gatagtcaaa acccttgaaa ttccgctgac
     2041 cagctggcag actgatctct gcttccactt caagctggac caaacctgtc agcttttgaa
     2101 aataggtctg ttcctcctga ctggttaatt tatagaaaac ctgataggtt tgtccgtcag
     2161 cccgaccacg aaaagacagg ctgtccccgt taatgtcaat agtatctgga atgacctgca
     2221 cagttgtgac ttgctctgtg gctgactggt ctgcccgctc ccattggacc ttttggtagc
     2281 cgaaaaagag accacataga gctaaaagcg gcagcgtttt gaggaaaacc gtcttacctt
     2341 gccgaacacc aaagacagcc agtaacagac tgaacaggcc cattgtcaag agggaaaaag
     2401 aatggactgc aaagtaggcc gccaccgcca agactgccag gtgaacgggc tggcagggga
     2461 gcttactcaa ccgtgacata aggccggatg ctttccatag tcttgtcgcc gatgcccgac
     2521 acattgttga ggtcgtccac cgacttgaag ccaccattgg cctcacgata ggcgataatg
     2581 tccgtcgctc gcttggcacc aataccagaa atggtctgca agtcagcctc ggtcgccgta
     2641 ttcagattga ccagactggt gcttttttct tctggggaca tagcagagct ggcagttgtg
     2701 ctagtcacca ccgagatgtt ttcgtcctta ctggccacat agaccaccgc ctcgtcgctg
     2761 agcttttgag ccagattgat agactttggg tctgcctgac tggtcaagcc gccagctgct
     2821 tcaactgcat cattaacacg cgcaccagct tctaaggtgt aaagccctgg tttatccacc
     2881 gctcctttga catcaacaac cagctggctg ggctccttac ttacttctgt actgatttcc
     2941 tccgtctgct cctgaccgct tgaagtttgt tcggtctgcg gaaaatcagt cagacccgtc
     3001 tgatcagact tggctggttg gctgaatatt aagaacgtcg ccattagcaa gccagccact
     3061 gctggcagag caatttgcca cttgtattct ttaagcaatt ctatataagt tttaatcgta
     3121 tccataaatt acctccacct atatagttcg taaaaaatac aaaaaaaccc gcaagctgcg
     3181 agtttcattt acttattttt tatccaactg agtacttggt tgccagaaga agctggcaat
     3241 atagctaaag gcataggtca atcccaagat aagagcaacc agcaaaagaa ctgggatacg
     3301 atagatataa gtcaagatat ttggtttttt cttggtttgg aaagatgccg catggtcatc
     3361 taagcgtttg aactcagcct caattcggct agttacttct gctgtacctg tatcatccat
     3421 acgcttgatg tccgaaacat cgataggatt tccaaaagcc acatcgattc gctcaccagc
     3481 taagagccct ttgatagtca tgggacccac ataggttgct ggcatgagtt tgaccttggc
     3541 tgacttggca atgaccgcca caccaccttt tagctcagat gaatgacgac ttccagaagg
     3601 aaacatgacc agcgaacggt cacttttttt caacatgtta actggatact tgatagctgc
     3661 catgccagga ttttcacggt caattggaaa ggctccacat tttgaaatcc accaaccaaa
     3721 accgcggtcc ttgaagagtt cttttttagc cataaaaatg aactgcttag gagcagctgc
     3781 gtagccaaga aagactggat cccagaaggt tttgtgcgga gcgataagaa tataattttc
     3841 ctcctgtggc aaaatcttct ctctatcatg atagtgaata ttgccattga tggcccacaa
     3901 caagaatgtt aatagtgttc gtagataaga ataaaacata aacttctcct tttcaatccc
     3961 tcctattata gcacaaattt gcgtcagtac gaaatgtcat attcattcaa atttacggtc
     4021 aaaataccca ctatttacat ggcccagact tgtcaaatat cctcgatagg tttacaatag
     4081 aagcatgatt acaattgaac gtgtattaga aatattgaag gcggatgcta atttccgcca
     4141 tatcaaacag aatgatcaga ctgacagcac atggacggat gtccagtttg acgctctcag
     4201 ctatgacagc cgcacggtta gcccgacgac cctctttttt gccaagggtt tggcctttaa
     4261 aaaggaattt ctggaaaaag ctatcgaggc cggactggct ttttacgtgt ctgaaatcga
     4321 ctatgaggtt gacattcctg ctatcatcgt ccatgacatc aagcaggcta tgagcctgat
     4381 tgccatggaa tttcatggtc atccccagaa acagctcaaa ctcctggcct tcaccggcac
     4441 caagggtaaa acaacggcgg cttattttgc ttttaacatt ctcaaacaga gccacaagcc
     4501 tgccatgctt tcgaccatga acaccacact ggacggtaag accttcttca agtccaccct
     4561 gaccacgccc gaaagcctgg acctctttgc catgatggcg gaggcggtta aaaacggcat
     4621 gagccacttg attatggagg tgtccagcca ggcttacttg gtcaagcggg tttacgggct
     4681 gacctttgac gtcggtgtct tcctcaacat cagccccgac cacatcggcc ccattgagca
     4741 tccgaccttt gaggactact tctaccataa gcgtcttttg atggacaata gcagagcggt
     4801 tattgtcaat gcaggaatgg accattttga agtcgtgaaa gagcaagtca gctccaaaga
     4861 ccatgacttt tatgggccga cttctgaaaa ccagattagc caatcagcag gttttgactt
     4921 cacagcgact ggcaaattag ctggccatta cgacatccag ctgattggtg gcttcaacca
     4981 agaaaatgcc atcgcagccg gcctagcctg tctccgcttg ggagcaagtc tggaagatat
     5041 tcacacaggg attgcccaaa ccaatgtccc tggtcgcatg gaagtcctaa cccagcaaaa
     5101 cggtgccaag gtattcgtgg actacgccca taacggcgat agtgtcaaga agctgattga
     5161 tgtcgtctta gagcaccaga caggcaaggt atttttgatt ttgggagctc ctggtaacaa
     5221 gggagaaagt cgccgcaaag acttcggtct tctgctcaat gactatccgc agattgaggt
     5281 gattctgaca gctgatgacc ccaaccgtga agacccagct gctattgctg aacaaatccg
     5341 tgcccacatg acccgtccaa gcaactttat cttggaccgg gaagaagcca ttcgcacagc
     5401 catgagtcag acaagctcta cgaaagatgc cgtcatcatc gcaggcaaag gggctgacgc
     5461 ctaccagatt gtaaacggcg aaaaggccgc ctatgacggt gatttggaag tagcaaaaca
     5521 atatttgtaa catacggtcc ttgttc
//
LOCUS       DBNFEG010000143         5534 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.143, whole genome shotgun sequence.
ACCESSION   DBNFEG010000143 DBNFEG010000000
VERSION     DBNFEG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.143"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1483
                     /gene="cls"
                     /locus_tag="AC1PNG_001715"
     CDS             <1..1483
                     /gene="cls"
                     /locus_tag="AC1PNG_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIP6702205.1"
                     /translation="IFLILWLVGSAATYMPALLIILQIFSIFVAISIINRPMNTSFKL
                     TWIIFVIGIPIFGALFYFILQSNIETKRYRKNFHKQAQRLREYSKTSEKVMKALEQED
                     KEQLKLAHFMSEYVGYPIHTNTDAVYFSTGQEKFEALLEELKKAEQYIFMEYFIVDMG
                     YMWDSILDILKEKAAQGVEVRFMYDGMNSLTNLPYNYYKTLRKYGIKAKVFSQIIPAL
                     STVQNNRDHRKIAIIDGKVAFTGGINIADEYINKKVRFGYWKDAAIMIRGEAVANFTL
                     MFLQMWNYNEKTETDDLKYLKTHKDLETETVDAEGFFLPYGENPFDGDEVAKRVCLDM
                     IQSATDYIYIMTPYLVLDDEMIDNLTYAAKRGVEVRILLPHIYDKKSAYLAARTDFPT
                     YLEAGIEIFEFQPGFVHSKVVLVDDKKATVGTVNMDFRSFYLNFECGLYIYNHSAVLA
                     AIKDDFTHSFKKSQQINLLTFHKTYSWYKRLGGALLRIISPLL"
     gene            1639..3852
                     /gene="recJ"
                     /locus_tag="AC1PNG_001716"
     CDS             1639..3852
                     /gene="recJ"
                     /locus_tag="AC1PNG_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIP6702206.1"
                     /translation="MIKPNYDWQLLTGFSDEQFIKIAKKEGVDPVAAKLLYERGIHSA
                     EELHTFLQPSLEDLHDPYLLHDMDKAVERIRRAIEDYEQILIYGDYDADGMTSASILK
                     ETLEEMGAEVQVYLPNRFTDGYGPNQSVYKYFIEQQGISLIVTVDNGVAGHEAIAYAQ
                     EMGVDVVVTDHHSMQETLPNAYAIVHPEHPEGNYPFKHLAGCGVAFKLACALLETVHA
                     DLLDLVAIGTIADMVSLTDENRVMVKYGLSLLKQTERAGLQELIKIAGIDIDSIDEET
                     VGFQLAPRLNALGRLDDPNPAIELLTGFDDEEAHQIALMIDSKNVERKDVVQAIYDEA
                     KTMLRQDRPVQVLAKEGWNPGVLGIVAGRLLEELQQPVIVLSIEDGKAKGSARSVEAV
                     DIFKALKDHQDLFIAFGGHAGAAGMTLEVDKLEELALTLTDYIIENKLDLSSKSSLVL
                     DEELDLEELTLDTLKSFEKLAPYGRDNKKPVFYIRDFQVESARTMGQNNAHLKLRISK
                     GAAGFDVVAFGKGNLALEFSQAKGLELAVTLSVNQWNGNTSLQLMLVDARVNGVQLYN
                     IRGKQHPIPAGVPILDIENPVADNKAIVLANLPEDLSSLRSYFQEREFEAIYFKNEIA
                     KPYYLDGYGSREQFAKLYKTIYQFDEFDVRYKLKDLAVYLKIKDSLLVKMIQIFQELE
                     FVTITDGVMRVNKDAQKREISESQIYQELKETVIQQELMALGTVQEIYEWLCGRV"
     gene            3831..4961
                     /locus_tag="AC1PNG_001717"
     CDS             3831..4961
                     /locus_tag="AC1PNG_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HIP6702207.1"
                     /translation="MAMWSRLMTVEALVVVGVIGTLLYTYWFIRQKSLLKNLEQITEI
                     KHALIELRRVGWSERAIKKVFLKQLLPLKQEDIPAFVQNFIKETTIFASTSFYQLIRV
                     DSRSLSQEKATALLEQSMNMLDFPEELSHGILPELLQKMDVNCPPHHPFWRYFAKLVD
                     KVFPVRELEVKRPLNRQVHQLRYFISYQQAFLVRQQFGKEKTDWQALIAYLRSLPRWF
                     YRLRESARLHNKQLFGKKNQKTLPVNMKILIHFHSEFILNQDGQFALILEERPHVNGV
                     VNGASFNYARANNKRHRQLDMAPVGRQDPVFRKELLRSKMGVYLSPTRFRRYQKGRNE
                     VGWEQSYFNQQGSFSYGGHSRAACVANLRRRFAKDIGLKCTK"
     gene            5015..5527
                     /locus_tag="AC1PNG_001718"
     CDS             5015..5527
                     /locus_tag="AC1PNG_001718"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIP6702208.1"
                     /translation="MNLKDYIATIPNYPKEGIEFRDISPLMADGNAYSYAVREIVQYA
                     TDKQIDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGIVAGCAFLIELDDLKGREAIG
                     DYDYKVLMHY"
BASE COUNT         1654 a          999 c         1257 g         1624 t
ORIGIN      
        1 cattttcctt attctttggt tggtaggttc ggcagctacc tatatgcctg ctttattgat
       61 tattttgcaa attttttcga tttttgtggc tatttcaatc atcaatagac cgatgaatac
      121 cagttttaag ttaacctgga ttatttttgt cattggtatt ccgatttttg gtgctctatt
      181 ctattttatt ttgcagtcca acattgaaac caaacgttat cgtaaaaatt ttcataagca
      241 agcacaaaga cttcgggagt atagtaagac gtctgaaaag gtcatgaagg cattggagca
      301 ggaagataag gagcaactga aattagctca cttcatgagt gagtacgttg gctacccaat
      361 tcataccaat acggatgccg tctatttttc aacaggtcag gaaaaatttg aagctctact
      421 agaagaattg aaaaaggcag agcagtatat tttcatggag tactttattg tcgatatggg
      481 atatatgtgg gattctatcc ttgacatttt gaaggaaaag gcagcgcaag gggtggaagt
      541 tcgttttatg tatgatggga tgaattcact gaccaatctt ccctataatt actataaaac
      601 cttgcgaaaa tatgggataa aagccaaagt attttctcaa attatcccag ccttatctac
      661 ggtgcaaaat aaccgtgacc atagaaaaat tgcgattatt gatggaaagg ttgcctttac
      721 cggtggcatt aatatcgcag atgaatacat caacaagaaa gtgcgttttg gttactggaa
      781 agacgcagcg attatgatta ggggagaagc tgttgcaaac tttacgttga tgttccttca
      841 aatgtggaat tacaatgaaa agacagagac agatgaccta aaatacctca aaacgcataa
      901 agatttagag acggaaaccg ttgatgccga aggtttcttc cttccgtatg gcgagaatcc
      961 atttgatgga gatgaggtag ccaaacgagt ctgcctagac atgattcagt cggcaacgga
     1021 ttatatttat atcatgacac cctacctagt gttggatgat gaaatgattg ataatttgac
     1081 ttatgcagcg aagcgtggag tcgaggttcg cattctacta ccgcatatct acgataaaaa
     1141 atcagcctat ttggctgctc gcactgattt tccgacttac ttggaagcag gaattgaaat
     1201 ctttgaattc cagcctggat ttgtccattc caaagttgtt ttggtcgatg ataagaaagc
     1261 tactgttggt acggtgaata tggattttcg ctctttttat cttaattttg aatgcggtct
     1321 ctatatttac aatcactcgg cagttttggc agctattaag gatgacttta ctcattcctt
     1381 caaaaaatca cagcaaatta accttctaac cttccataaa acgtactctt ggtataagcg
     1441 tttgggagga gccctactac gaattatttc gccgttatta taaattgcaa tattcgttct
     1501 tcatggagca agggatcgac attctgtttc ggtcttacct tgctccattt ttcttggaaa
     1561 atgatataat agaaagatag tttatactcg gaaaatttac aggctacttg tgattttgag
     1621 tattaaggag taaaaaacgt gataaaaccc aattatgact ggcaattatt gaccggtttt
     1681 tctgatgaac aatttatcaa aattgctaaa aaagaaggag tggacccggt agcggctaag
     1741 ttgctgtatg aacgtggcat ccattcggcg gaagaattgc atactttctt acaaccaagc
     1801 ttagaagatc ttcacgatcc ctatttacta catgatatgg acaaggcggt agagcggatt
     1861 cgccgagcaa tcgaagatta cgaacaaatt ttgatttatg gagattatga cgcagacggc
     1921 atgacctcgg catctattct gaaagaaact ttggaggaaa tgggggcaga agtccaagtt
     1981 tatcttccca accgtttcac agatggctat gggccgaatc agtcagttta caagtatttt
     2041 atcgagcaac aggggatttc tcttattgtg acggtggaca atggcgtagc gggtcatgaa
     2101 gcgattgcct atgcccagga aatgggtgtc gatgttgtcg tgacagacca ccattccatg
     2161 caggaaaccc ttcccaatgc ctatgcgatt gtacatccag agcatccaga gggaaattat
     2221 cccttcaaac atttggcggg ctgcggtgtg gcttttaaat tggcctgtgc tcttcttgaa
     2281 actgttcatg cagacttgct ggatttggtc gcaattggca ccatcgctga tatggttagc
     2341 ttgaccgatg agaatcgtgt catggtcaaa tatggtctct cgcttctcaa acagactgag
     2401 cgagcaggtt tgcaagagtt gataaagata gctggtattg acatagacag tattgatgaa
     2461 gaaacagttg gtttccaact tgctccgagg ttgaatgccc ttggtcgact cgacgatcct
     2521 aatccagcca tcgaattact gactggtttt gatgacgaag aggcccatca aattgctctc
     2581 atgattgaca gtaaaaatgt agaacgcaaa gacgtcgtcc aagcaattta cgatgaagca
     2641 aaaaccatgc tccgtcagga tagaccggtt caagttctgg ctaaagaagg ttggaaccca
     2701 ggtgttttag ggattgtagc aggacgccta ctagaagaat tgcagcaacc tgttatcgtc
     2761 ttgtctattg aagatggtaa ggcaaagggg tcggctcgtt cggttgaagc agttgacatt
     2821 ttcaaggctc ttaaagatca tcaggaccta tttatcgcct ttggtggtca tgctggtgca
     2881 gcaggtatga ccttggaagt tgacaaactt gaagaacttg ctctgacctt gacagactac
     2941 attatcgaaa ataaattgga tttatccagc aagtcttcgc tggtcttaga tgaagaattg
     3001 gatttagagg aattaaccct agatactcta aaatctttcg agaagcttgc gccttacggc
     3061 agggataata aaaagcctgt attctacatc agagactttc aggtggaatc agcaaggacc
     3121 atggggcaga ataatgccca tttgaaacta cgcattagta aaggtgcggc aggttttgat
     3181 gtggttgctt ttggtaaggg caatctagcc ttggaatttt cacaggccaa gggcttagaa
     3241 ctagctgtca ccttgtctgt caatcaatgg aatggcaata ccagtcttca gctcatgttg
     3301 gttgatgctc gggtcaatgg tgttcaactt tataatattc gtggaaagca acatcctatt
     3361 cctgctggag tcccaatttt ggatattgaa aatccagtag cagataacaa agcaattgtc
     3421 ttggctaact tgcctgagga cctcagtagt ctgcgtagct attttcagga aagagaattt
     3481 gaagccattt attttaaaaa cgaaattgcc aaaccgtact atctagatgg ttatggtagc
     3541 cgagaacaat tcgctaaatt gtataagact atttaccaat ttgatgagtt tgatgttcgc
     3601 tacaaactga aagatttggc tgtttactta aaaattaagg acagtctatt ggttaaaatg
     3661 attcagattt tccaagaatt agaatttgtc acgattacgg atggggtcat gcgggtcaac
     3721 aaagatgcac agaagcgaga gatttcagaa agtcaaatat atcaagaatt gaaagaaaca
     3781 gttatccaac aagagttgat ggcgcttggc accgttcagg aaatttatga atggctatgt
     3841 ggtcgcgttt gatgacagta gaagctctgg ttgttgttgg agtgattgga acgcttctct
     3901 acacttattg gtttatcagg caaaaaagtc tgttgaaaaa tcttgaacag ataaccgaaa
     3961 taaagcacgc actgattgag ttacggcgag taggatggtc agaaagagcc atcaaaaagg
     4021 ttttcctgaa acaactgttg cctctaaaac aagaagacat cccagcattt gttcaaaatt
     4081 tcataaaaga aactactatt tttgcctcga ccagctttta tcagttgata agagttgaca
     4141 gtcgctcttt aagccaagag aaagcgacgg ctttattgga gcaaagtatg aatatgttag
     4201 attttcctga ggaattaagt cacgggatat tacctgaact tttgcagaag atggatgtga
     4261 attgtcctcc acaccatccg ttttggcggt actttgctaa gctagttgat aaggtttttc
     4321 cagtgcggga attagaagtc aaacggccgt taaacagaca ggtccaccaa ctgcgctact
     4381 ttatttccta tcagcaagct tttttggtac gtcagcagtt tggaaaagaa aaaacagatt
     4441 ggcaggctct gatagcctat ttacgtagcc tacctcgatg gttttaccgc ttacgagaat
     4501 ctgcccgcct acacaataaa caactgttcg gcaagaaaaa tcagaagact ttgccagtca
     4561 atatgaagat tttgattcat tttcacagtg aatttatcct aaatcaagat ggacaatttg
     4621 ccttgatttt agaagaacga cctcatgtca atggcgtggt aaacggtgct tcctttaatt
     4681 atgccagagc taataataag cgtcatagac aacttgatat ggctccagtt ggcaggcagg
     4741 atcccgtttt tcggaaagaa ttgctacgct caaagatggg agtctattta tcgcctactc
     4801 gctttcgtcg ctaccaaaaa ggaagaaatg aagttggctg ggagcagtct tactttaatc
     4861 agcaaggaag cttttcctat ggtgggcata gcagggcagc ttgtgtggcg aatttgagga
     4921 gacgatttgc taaagatata ggtctgaaat gtaccaaata acaatttcat ggtataatga
     4981 agagtaaaaa ttatttttaa aaagagagag acaaatgaac ttaaaagatt acatcgcaac
     5041 aattcctaac tatccaaaag aaggtatcga attccgtgac attagccctt tgatggcaga
     5101 tggaaatgct tatagctatg cagtacgtga aatcgttcag tatgcgacag ataagcaaat
     5161 cgatatgatt gttgggccag aggcgcgtgg tttcattgtt ggatgtccag ttgcctttga
     5221 attgggaatc ggttttgcac cagttcgtaa gccaggtaag ttaccacgtg aggttatctc
     5281 agctgactat gaaaaagaat atggtgtaga tacattgacg atgcatgcgg atgcgattaa
     5341 gccaggtcaa cgtgttctta tcgttgatga cttgttggca actggtggta ctgtaaaagc
     5401 aacgatcgaa atgattgaaa agcttggcgg tatcgttgct ggctgtgcct tcttgattga
     5461 gctagatgat ttgaaaggcc gcgaagcaat cggtgattac gactacaagg ttttgatgca
     5521 ctattaatct aagg
//
LOCUS       DBNFEG010000144         5358 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.144, whole genome shotgun sequence.
ACCESSION   DBNFEG010000144 DBNFEG010000000
VERSION     DBNFEG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.144"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..937
                     /locus_tag="AC1PNG_001719"
     CDS             <1..937
                     /locus_tag="AC1PNG_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702209.1"
                     /translation="FKGRSMVWTEEKLRPIIHSILNPDEEVLSLYQSKTTKSIYGCIL
                     WNSYYYKVFRVSNHPSQSTYKQPTFYDFHGEFYMKGAIRTYLYHTNSWYELSKQSYYL
                     LLFFQKLWDEKQEVEASWQNGRMQIRLVVEEEGWQVHYRFPDNQAREVERLLASGMLV
                     AIRSSRNFIKIRTSRFVAPYIALIKQRQLYRKKPSKAMLQWEKYQSQLIEIQRTYRLV
                     EENAPKTFFGHWLNKVQLYLCSAIASYWEEVIKGVEWQEAREIKDQRKIEKPHNPIED
                     KWKENIARRHKRKVDQLIGHDTLEKLRQLKTELDD"
     gene            1148..2131
                     /locus_tag="AC1PNG_001720"
     CDS             1148..2131
                     /locus_tag="AC1PNG_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HIP6702210.1"
                     /translation="MSKDIRVLLYYKYVPIENAKEYAAEHLAFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDYVHANPLFSDLWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDIDPLVTTGAYLSPKEFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVRGELWDGKMYVFDERIAVDVNHVNPVVVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEHKYVRGCSAECRAHERNRYISENGLTRQEWAERLEAIGETLTPANA"
     gene            2451..2711
                     /locus_tag="AC1PNG_001721"
     CDS             2451..2711
                     /locus_tag="AC1PNG_001721"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIP6702211.1"
                     /translation="MTLPEGGNLILMQADSFAQRVPFQVVASGNEVLISLNVASRKEV
                     DTLIERVEANSGQITGCPTDARGFYGASFTDLDGHHFNVIVM"
     gene            2818..4491
                     /locus_tag="AC1PNG_001722"
     CDS             2818..4491
                     /locus_tag="AC1PNG_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6702212.1"
                     /translation="MKKIIEFKDFTFKYQAQQEPTLKDIQLTIYQGEKVLIIGPSGSG
                     KSTIGQCLNGIIPNIYKGEHSGSLLLDGQEAFNLSVYDKSLLVSTVLQDTDGQFIGLS
                     VAEDLAFALENDMESQVVMKQKVGDWAERLSLTDLLAHRPQDLSGGQKQRVSLGGVLI
                     DESPILLFDEPLANLDPKSGQDTIDLIDRLHKQEGTTTIIIEHRLEDVLYRQVDRVVL
                     INDGRILFNGGLDDLLRTSLLQENGIREPLYLSTLRALGYPIAQAEHLSSVWEIDIEA
                     LNIASLPTLHFENGEKESLLEVKHLHFSYPNKQVLTDIQLTIHKGERLAIVGKNGAGK
                     STLAKVLCGFIQGEGEILWKGQSIKEDSVKERAERIGYVLQNPNQMISQTMIFDEVAL
                     GLRVRGVTEEEIEHRVHHILKVCGLYPFRNWPISALSYGQKKRVTIASILVLNPEIIL
                     LDEPTAGQDQRHYREMMEFLDQLNAQGHTIVMITHDMQLMLDYSDRAVVVVDGQIIED
                     ASPAEILSDDMVIERANLKETSIFHLAERLGVNPLELTTFYMQEERRGR"
     gene            4488..5320
                     /locus_tag="AC1PNG_001723"
                     /pseudo
     CDS             4488..5320
                     /locus_tag="AC1PNG_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
BASE COUNT         1576 a          964 c         1212 g         1606 t
ORIGIN      
        1 gttcaagggg agaagtatgg tctggacaga agaaaagttg agaccgatta ttcattctat
       61 actcaatcct gacgaggaag ttttaagtct ttatcaatcg aaaacgacga aatcaattta
      121 tggctgcatt ctttggaata gttattatta taaggttttc cgagttagta accacccatc
      181 acaatctaca tataaacagc caacttttta tgatttccat ggcgaatttt atatgaaagg
      241 agcgattcgg acctaccttt atcacacgaa cagttggtat gaacttagta aacaaagcta
      301 ctaccttctt ctcttttttc aaaagttatg ggatgaaaag caagaggtgg aggcgagttg
      361 gcaaaatggt agaatgcaga ttcgcttagt ggttgaagaa gaggggtggc aagtccatta
      421 ccgttttcca gataatcagg caagggaggt tgaacgctta ttggcctcag gcatgctggt
      481 ggccattcgc tcctccagaa attttatcaa gatacggacg agccgttttg ttgcaccgta
      541 tatagctcta atcaaacagc gtcaacttta tcgaaaaaag ccgagtaaag ctatgctcca
      601 gtgggaaaaa taccaaagtc aacttattga aatccaaagg acctatcggc tagtggagga
      661 aaatgctccg aaaacttttt ttggacactg gttgaataag gtccaactct atctctgctc
      721 tgccattgcc agctattggg aagaagttat taaaggagtg gaatggcagg aggcaagaga
      781 aatcaaagat caaaggaaaa tcgagaagcc acataatcct attgaagata aatggaagga
      841 aaatattgct agacgccata agcgaaaagt ggaccaactg attggacatg ataccttgga
      901 aaaactccga caactgaaaa cagaattaga cgactaagaa gttatagcga accttcaata
      961 aatgcggtag catgccatcc atgttaccgt attttttaca tataagcatt gaacccgcga
     1021 atacaggttc taaattttca gttcagattt ttgtcttttg aggggctaaa cgatataatc
     1081 gtaaggtatc atttattaga aatgacagag ttttatcatc tattgcaaag attgaaaagg
     1141 agacactatg tcaaaagata ttcgcgttct actctattac aagtatgttc caattgaaaa
     1201 tgccaaagaa tatgctgctg agcatctagc tttctgtaag tctatcggac tcaaaggacg
     1261 tattttgatt gctgatgagg gaatcaacgg tactgtttct ggtgactatg aaacaactca
     1321 aaagtacatg gactacgttc atgcaaaccc attgttcagc gatttgtggt ttaagattga
     1381 tgaagaaaat gagcaagctt tcaagaaaat gtttgttcgt tataaaaaag aaattgttca
     1441 ccttggtttg gaagacaacg actttgacaa cgatattgat ccactcgtga caacaggtgc
     1501 ctacctatca ccaaaagagt tcaaagaagc tctcttggac gaagatacag ttgttttgga
     1561 tacacgtaat gactacgagt atgaccttgg tcacttccgt ggtgcgatcc gtccagacat
     1621 ccgcaacttc cgtgagttgc cacaatgggt ccgtgataac aaagagaaat tcatggacaa
     1681 gcgcgtagtt gtttactgta ctggtggtgt tcgctgtgaa aaattctcag gctggatggt
     1741 tcgtgaaggc tacaaggatg tcggccagct ccacggtggt atcgcaacct atggtaaaga
     1801 cccagaagtt cgtggcgaat tgtgggatgg gaagatgtat gtctttgacg agcgtatcgc
     1861 ggtcgatgtt aaccatgtta accctgttgt cgttggtaag gactggttcg atggcacacc
     1921 atgtgagcgc tatgtcaact gtggcaatcc attctgtaac cgtcgcattt tgacatcaga
     1981 agagaatgag cacaagtatg ttcgtggctg ttcagctgaa tgccgtgctc atgagcgcaa
     2041 ccgatacatc tctgaaaacg gcttgactcg tcaagaatgg gcagaacgct tggaagcgat
     2101 tggggaaact cttactccag caaatgctta gtaataccaa aattgcattg aaaaatcagt
     2161 gcaatttttt tgtgcaatca gaattcttta tattttttca gtccattcta tacgaagcct
     2221 atcaattgga aaagagaagg agatgaagtc attttggatg aacttatccc tttcgaaaat
     2281 caacattatc cgttgtcagc ttgccttgat gaacttcagt tctatcttcg cttcgtttcc
     2341 taggttactt ttgattttca ttgagtatta gcagtggcag accttgaata gctggtcggt
     2401 tttatgaagc aatcggtttt gcagttgcga cgtttggaga tattaagtct gtgactttgc
     2461 ctgagggtgg caacttgatt ttgatgcagg cagatagctt tgctcaacgc gtaccatttc
     2521 aggtagtagc gagtggcaat gaagtcttga tttcgcttaa tgtggcgagt aggaaagaag
     2581 tggatacact tatagagcgt gttgaagcaa atagcgggca aattacagga tgcccgacag
     2641 atgcccgtgg cttttatgga gcaagtttta cggaccttga tgggcatcat tttaatgtga
     2701 ttgtgatgta ggaagttaga attcatctac tctagaatca tagatgaagt tatttgaaaa
     2761 gggagaaact gatgattctt cctttttttc agagtcaatt tacgctataa tagtcctatg
     2821 aaaaaaatca ttgaatttaa agattttaca tttaaatacc aagcgcaaca ggaacccact
     2881 ttaaaagata ttcagttaac gatttatcag ggagagaaag ttctgattat tggcccatct
     2941 ggttcaggaa agtcgacgat tggacaatgt cttaatggta ttattcccaa tatttacaag
     3001 ggggaacata gcggaagttt actattggat ggtcaggaag cttttaatct ttcagtttat
     3061 gataagtcct tgctcgtatc aacggttctt caagatacag atggtcaatt cattggctta
     3121 tcagttgccg aggacctagc ttttgctttg gaaaacgaca tggaaagcca ggtggtgatg
     3181 aagcaaaaag taggagattg ggcagaaaga ctgtctttga ccgatttgct ggcacatcga
     3241 ccacaagatt tatctggagg tcaaaagcaa cgtgtcagtc tgggcggagt tcttattgat
     3301 gagagtccta ttttgttgtt tgatgagcca cttgctaatt tagaccccaa atcaggccaa
     3361 gatacgattg atttgattga ccgtctgcac aagcaagaag ggaccacaac cattattatc
     3421 gaacaccgtt tagaggacgt actgtatcgt caggtggacc gagttgtatt gattaatgat
     3481 ggtcggattt tgttcaatgg aggactggat gatttattac gtacctcatt gttacaggaa
     3541 aacggcatcc gtgaaccctt atatctatcg actcttcgcg ccttaggata tccgattgcg
     3601 caggcagaac acctctcctc agtttgggaa attgatattg aggctttgaa tatagcatct
     3661 ctaccgactc tacactttga aaatggagag aaagagtctt tgttagaagt caagcatctt
     3721 catttttcct atcctaacaa acaagttctt acagatattc aactgacgat tcataaggga
     3781 gagcgtctag ctattgttgg aaaaaatggt gctgggaaat ctactttagc caaggtgctt
     3841 tgtggtttta ttcagggtga gggtgagatt ctctggaagg ggcagtcaat caaagaagat
     3901 tctgtcaaag agcgagcaga acggattggt tatgttcttc agaatcccaa ccaaatgatt
     3961 agccaaacga tgatctttga tgaggttgct cttggtttgc gcgtgcgagg ggtgacagaa
     4021 gaagaaatcg aacaccgagt tcaccatatc ttgaaagtat gtggcctgta tccgtttcgt
     4081 aattggccga tttcggcact ttcctatggg cagaaaaaac gggtaacgat agcatctatc
     4141 ttggttctca atccagaaat tattttgcta gatgagccaa cagcaggaca agatcaacgg
     4201 cattatcggg aaatgatgga gtttttggac caattaaatg ctcaagggca caccattgtc
     4261 atgattaccc atgacatgca actcatgcta gattattcag atagagctgt tgttgtggtg
     4321 gatgggcaga ttattgaaga tgccagtcca gcagaaattt tatctgatga tatggtgatt
     4381 gagcgagcca atttaaaaga aacatcaatt tttcacttgg cggagaggtt gggagttaat
     4441 cccttggaat taacgacgtt ttatatgcag gaagaaagga gaggaagatg agtcaacaaa
     4501 aactaattgg ttaccatcca ggaactggct ttattcatag tttatcggct gtcagtaaat
     4561 tacttttttt ctcatagttt ccattcttgc tatgattact tatgataccc gcttgattct
     4621 ttttatcgca gtattttctc tagccttgtt caaaatgtca gggattcgtt ataaagaaat
     4681 ctctcttgtt ttaattctga caatcatttt tgctgcaatg aatgccctga tggttcactt
     4741 gtttgcacca cggtatggag tggaacttta cggagcagac actcccttgc tatcaggtct
     4801 gggagtttat tccttgacga gtcagcaagc attttatctg gtgaatcttc tcttgaagta
     4861 tttttgtaca gttcctttag cgattatctt tctcatgacc actcatccta gtcagtttgc
     4921 ttccagcttg aatcaaattg gggtatccta caaagttgct tacgcagtca gtttgaccat
     4981 gcgttatatt ccagatattc aagaagagtt ttatacgatt cgaatgtctc aagaagcgcg
     5041 tggtttggaa ttgtccaaga aaggaaaatt gatggatcgt ataaaaggga atctatccct
     5101 tgtcattcct ctgattttca gctccttgga gaggattgat acgatttcaa cggctatgga
     5161 attgcggcgt tttggaaaaa ataaaaaaag aacatggtat acccaacagc ctctacaaag
     5221 aatagattac gctgtcttgc tatttattct tgctctagta gtcgtgacaa tctatctatt
     5281 ttttattaat cagggtcgat tctataatcc gtggcgatga ttattgagtt tattgggctt
     5341 atcaaccctt tatcgttg
//
LOCUS       DBNFEG010000145         5338 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.145, whole genome shotgun sequence.
ACCESSION   DBNFEG010000145 DBNFEG010000000
VERSION     DBNFEG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.145"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            174..1722
                     /locus_tag="AC1PNG_001724"
     rRNA            174..1722
                     /locus_tag="AC1PNG_001724"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1776..1848
                     /locus_tag="AC1PNG_001725"
     tRNA            1776..1848
                     /locus_tag="AC1PNG_001725"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1809..1811,aa:Ala,seq:tgc)
     gene            2151..5054
                     /locus_tag="AC1PNG_001726"
     rRNA            2151..5054
                     /locus_tag="AC1PNG_001726"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5243..>5338
                     /gene="rrf"
                     /locus_tag="AC1PNG_001727"
     rRNA            5243..>5338
                     /gene="rrf"
                     /locus_tag="AC1PNG_001727"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1508 a         1099 c         1525 g         1206 t
ORIGIN      
        1 caagaaatga taaactaaga tagttgtcgc gagaacgtga taacgacaag acctttgaaa
       61 attaaagaag acgaaccaaa cgtgcagggt gatttatcta aggataaatc gtcaatgaca
      121 aaacaaaaca ataaaacgga aagctagcaa tagcttgagt ttgaatcaaa actttttatg
      181 agagtttgat cctggctcag gacgaacgct ggcggcgtgc ctaatacatg caagtagaac
      241 gctgaagtct ggtgcttgca ctagacggat gagttgcgaa cgggtgagta acgcgtaggt
      301 aacctgcctc atagcggggg ataactattg gaaacgatag ctaataccgc ataacagtat
      361 ttaccgcatg gtagatgctt gaaaggagca actgcttcac tatgagatgg acctgcgttg
      421 tattagctag ttggtgaggt aacggctcac caaggcttcg atacatagcc gacctgagag
      481 ggtgatcggc cacactggga ctgagacacg gcccagactc ctacgggagg cagcagtagg
      541 gaatcttcgg caatgggggc aaccctgacc gagcaacgcc gcgtgagtga agaaggtttt
      601 cggatcgtaa agctctgttg taagagaaga actgtgagaa gagtggaaag tttctcactt
      661 gacggtatct taccagaaag ggacggctaa ctacgtgcca gcagccgcgg taatacgtag
      721 gtcccgagcg ttgtccggat ttattgggcg taaagcgagc gcaggcggtt tgataagtct
      781 gaagtaaaag gctgtggctt aaccatagta cgctttggaa actgtcaaac ttgagtgcag
      841 aaggggagag tggaattcca tgtgtagcgg tgaaatgcgt agatatatgg aggaacaccg
      901 gtggcgaaag cggctctctg gtctgtaact gacgctgagg ctcgaaagcg tggggagcga
      961 acaggattag ataccctggt agtccacgcc gtaaacgatg agtgctaggt gttgggtcct
     1021 ttccgggact cagtgccgca gctaacgcat taagcactcc gcctggggag tacgaccgca
     1081 aggttgaaac tcaaaggaat tgacgggggc ccgcacaagc ggtggagcat gtggtttaat
     1141 tcgaagcaac gcgaagaacc ttaccaggtc ttgacatccc gatgaccgcc ctagagatgg
     1201 ggtttctctt cggagcatcg gtgacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga
     1261 gatgttgggt taagtcccgc aacgagcgca acccctattg ttagttgcca tcattcagtt
     1321 gggcactcta gcgagactgc cggtaataaa ccggaggaag gtggggatga cgtcaaatca
     1381 tcatgcccct tatgacctgg gctacacacg tgctacaatg gctggtacaa cgagtcgcaa
     1441 gtcggtgacg gcaagctaat ctcttaaagc cagtctcagt tcggattgta ggctgcaact
     1501 cgcctacatg aagtcggaat cgctagtaat cgcggatcag cacgccgcgg tgaatacgtt
     1561 cccgggcctt gtacacaccg cccgtcacac cacgagagtt tgtaacaccc gaagtcggtg
     1621 aggtaacctt ttaggagcca gccgcctaag gtgggataga tgattggggt gaagtcgtaa
     1681 caaggtagcc gtatcggaag gtgcggctgg atcacctcct ttctaaggaa atggaaacct
     1741 gtacgtcagt cttctttaat tttgagaggt cttgtggggc cttagctcag ctgggagagc
     1801 gcctgctttg cacgcaggag gtcagcggtt cgatcccgct aggctccatt aggatagaaa
     1861 tcctactaaa ctgaataaca gtgaagttga attcacgact aacttcttag gaaaatagat
     1921 aatcttcctt gtgtgcaaga cacacaatgt cagattccta attttctaca gaagtttcgc
     1981 agaagcgacc gtcgttcata tcctaaactt gtccattgaa aattgaatat ctatcaaaca
     2041 ttcctaaacg tatgtaaaaa tacgtataga aatagtaaca agaaaataaa ccgaaaacgc
     2101 tgtgaatatt taatgagttt tctaattttt gaaaaaaatt aggttaataa ggttaagtta
     2161 ataagggcgc acggtggatg ccttggcact agaagccgat gaaggacgtg actaacgacg
     2221 aaatgccttg gggagctgta agtaagcaat gatccaggga tgtccgaatg ggggaacccg
     2281 gcaggtaatg cctgtcactc actactgtta aggtagtgaa gaggaagacg cagtgaactg
     2341 aaacatctaa gtagctgcag gaagagaaag caaaagcgat tgccttagta gcggcgagcg
     2401 aaacggcagg agggcaaacc gaggagttta ctcctcgggg ttgtaggact gcaatgtgga
     2461 cttaaagagt atagaagaac tacctgggaa ggtaggccaa agagagtaat agcctcgtat
     2521 ttgaaatatt ctttatacct agcagtatcc tgagtacggc gagacacgcg aaatctcgtc
     2581 ggaatccggg aggaccatct cccaacccta aatactctct agtgaccgat agtgaaccag
     2641 taccgtgagg gaaaggtgaa aagtaccccg gaaggggagt gaaatagaac ctgaaaccgt
     2701 gtgcctacaa caagttcgag cccgttaatg ggtgagagcg tgccttttgt agaatgaacc
     2761 ggcgagttac gatatgatgc gaggttaagt tgaagagacg gagccgtagg gaaaccgagt
     2821 cttaataggg cggattagta ttatgtcgta gacccgaaac catgtgacct acccatgagc
     2881 aggttgaagg tgcggtaaga cgcactggag gaccgaacca gggcacgttg aaaagtgctt
     2941 ggatgacttg tgggtagcgg agaaattcca aacgaacttg gagatagctg gttctctccg
     3001 aaatagcttt agggctagcg tcgacatttg agaatcttgg aggtagagca ctgtttggat
     3061 gagggggcca tctcggttta ctgatttcag ataaactccg aatgccaatg atttatggtc
     3121 ggcagtcaga ctgcgagtgc taagatccgt agtcgaaagg gaaacagccc agaccaccag
     3181 ctaaggtccc aaaataattg ttaagtggaa aaggatgtgg ggttgcacag acaactagga
     3241 tgttagctta gaagcagcta ttcattcaaa gagtgcgtaa tagctcacta gtcgagtgac
     3301 cctgcgccga aaatgtaccg gggctaaaac aatttaccga agctgtggat aacactttag
     3361 tgttatggta ggagagcgtt ctatgtgtga agaaggtata ccgtgaggag tgctggaacg
     3421 catagaagtg agaatgccgg tatgagtagc gaaagatggg tgagaatccc atccaccgta
     3481 agactaaggt ttccagggga aggctcgtcc gccctgggtt agtcgggacc taaggagaga
     3541 ccgaagggtg tatccgatgg acaacaggtt gatattcctg tactagagta tgaagtgatg
     3601 gagggacgca gtaggctaac taaagcgggc gattggaagt gcccgtctaa gcagtgaggt
     3661 gtgatatgag tcaaatgctt gtatctgtaa cattgagctg tgatggggag tgaagttaag
     3721 tagcgaagtt agtgacgtca cactgccgag aaaagcttct agcgatgtat catactctac
     3781 ccgtaccgca aaccgacaca ggtagtcgag gcgagtagcc tcaggtgagc gagagaactc
     3841 tcgttaagga actcggcaaa atgaccccgt aacttcggga gaaggggtgc tggcttaaag
     3901 tcagccgcag tgaataggcc caagcaactg tttatcaaaa acacagctct ctgctaaatc
     3961 gtaagatgat gtataggggg tgacgcctgc ccggtgctgg aaggttaaga ggagggttta
     4021 gcgcaagcga agatctgaat tgaagcccca gtaaacggcg gccgtaacta taacggtcct
     4081 aaggtagcga aattccttgt cgggtaagtt ccgacccgca cgaaaggcgt aatgatttgg
     4141 gcactgtctc aacgagagac tcggtgaaat tttagtacct gtgaagatgc aggttacccg
     4201 cgacaggacg gaaagacccc atggagcttt actgcagttt gatattgagt atctgtacca
     4261 catgtacagg ataggtagga gcctacgaag tcgggacgcc agtttcgacg gaggcgctgt
     4321 tgggatacta cccttgtgtt atggctactc taacccggat aggttatccc tatcggagac
     4381 agtgtctgac gggcagtttg actggggcgg tcgcctccta aaaggtaacg gaggcgccca
     4441 aaggttccct cagaatggtt ggaaatcatt cgcagagtgt aaaggtataa gggagcttga
     4501 ctgcgagagc tacaactcga gcagggacga aagtcgggct tagtgatccg gtggttccgt
     4561 atggaagggc catcgctcaa cggataaaag ctaccctggg gataacaggc ttatctcccc
     4621 caagagttca catcgacggg gaggtttggc acctcgatgt cggctcgtcg catcctgggg
     4681 ctgtagtcgg tcccaagggt tgggctgttc gcccattaaa gcggcacgcg agctgggttc
     4741 agaacgtcgt gagacagttc ggtccctatc cgtcgcgggc gtaggaaatt tgagaggatc
     4801 tgctcctagt acgagaggac cagagtggac ttaccgctgg tgtaccagtt gtcttgccaa
     4861 aggcatcgct gggtagctat gtagggacgg gataaacgct gaaagcatct aagtgtgaaa
     4921 cccacctcaa gatgagattt cccataactt tatgttagta agagccctga gagatgatca
     4981 ggtagatagg ttggaagtgg aagtgtggcg acacatgtag cggaccaata ctaatcgctc
     5041 gaggacttat ccaaactaag taacgaaggc gtcaaatccg cctgaaaaat aggaacctaa
     5101 cgcagtatgc ttgcatacaa ggcaggttat cttttttcca aggatttagc ccgagtacaa
     5161 ttactcacga gtcaatattg acaagcgatc ggtttcttgt tagaatatag atattcaatt
     5221 ttgagttgac aagactcatt agttaagtga cgatagccta ggagatacac ctgtacccat
     5281 gccgaacaca gcagttaagc cctagaacgc ctgaagtagt tgggggttgc cccctgtt
//
LOCUS       DBNFEG010000146         5307 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.146, whole genome shotgun sequence.
ACCESSION   DBNFEG010000146 DBNFEG010000000
VERSION     DBNFEG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.146"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(52..1764)
                     /locus_tag="AC1PNG_001728"
     CDS             complement(52..1764)
                     /locus_tag="AC1PNG_001728"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6702213.1"
                     /translation="MNRRLVGVLLKSLNKTMFLFGIALSALGVGMGLFLPQFIGQLLD
                     QTYLSNLLTRPELLAGFILFFVSVYTVQALSNYFIGRSGGNALKQLQQYIYESLLTTY
                     VKDLDQYQSGDLASRLTNDMSVVLNFITVILPNFLMNGLMVIGSIYFLWTISPWLTGL
                     SLLLLPMLSMVMIPMNQRLEGYYSAYQEGLGQVSSRISHKFTTIRLMKAFQGEKHEQR
                     EMGKSFQKLSQTFERMIRLSAVQHTLVSSLMTGFIILMLLVAGIEVTKGVMTMATLTT
                     FVLYMMQLIDPVTDIASSLNELTEFQAVSKRLVELLELTKEEQADTELVDEASIQLEN
                     VRFSYHTDPVLNGVSVRIPEGAHVAIVGPSGAGKSTIFALLMKYYQDYQGEIRIGQQC
                     LSDISTKEMRRLISFIPQDNTLFHGTIRENLLYGKNESVSEERIAHILKELGLSHLVA
                     ELEDGLDTRISENGTGLSEGQKQRFSIARALLLEHPIYLLDEATASLDTVTERVISKA
                     IDRLTAGKTRLTIAHRLHTVREADAILVLDKNGQVADYGPHQQLVERNHLYQDFLRGL
                     PQAS"
     gene            complement(1767..2537)
                     /locus_tag="AC1PNG_001729"
     CDS             complement(1767..2537)
                     /locus_tag="AC1PNG_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702214.1"
                     /translation="MKQIFPAIFKTIWKRKETQIYLLFTLFPFIYLVTSFIEGSNFMQ
                     IHASEGSVSLVAFTNMMMSSVDSFILPSLTLYFLAISVFRKEVDEHTMFLYRDLGRKQ
                     IFWSKYLGLLATIVIFYGLFFATSAFVHYVRVIHLPFGSPSVFDTSLAESLSNVFCIV
                     AYLLKDILSVSLATALCLYLKNITTMIIGFLVTITMMILAVVGGPVAMIFPTSYMPLA
                     SEGLTGVGLAYLGAIGVTLVYALVFTKIAAKKFKNLEF"
     gene            complement(2543..3415)
                     /locus_tag="AC1PNG_001730"
     CDS             complement(2543..3415)
                     /locus_tag="AC1PNG_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6702215.1"
                     /translation="MLKINHLSKQFAGNEFYSLKDVSLEINKGEIVGLIGKNGAGKST
                     LMKLIAKSLKPSSGTITYKGTDIFSQDNLLADFGIMIDPVFYPEMTVMDNLKFYLDLH
                     GKKDLYPNIEKTLKLVELWEARNRKPKGFSFGMKQRTALAIALVAEPDFLILDEPFVG
                     LDPIGVQKLIDILKQWSSERQISMLISSHQLGELEALCSRYVYIESGELAEAFEGKSH
                     PSVLVQLNTTKNLDSVEALLNDHVVLEGEVLEISTATPTSELNQIFGVLARQELIVKL
                     EVKENHLKEIFTKG"
     gene            complement(3408..3836)
                     /locus_tag="AC1PNG_001731"
     CDS             complement(3408..3836)
                     /locus_tag="AC1PNG_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msa family membrane protein"
                     /protein_id="HIP6702216.1"
                     /translation="MIFILLLSLLYTCLTVVSSQLLLSLSVPILLLLTYLLPPIVNSI
                     CLGLLKDNSWKAWSAGIFPTMSMIFYAAFAYITSSNGQWAQFSQLNTVSDDVMSVEVA
                     SNLLAPSQILFVAVVYYGVAIATYFIQQTSRQKEKGVTYA"
     gene            complement(3851..4429)
                     /locus_tag="AC1PNG_001732"
     CDS             complement(3851..4429)
                     /locus_tag="AC1PNG_001732"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIP6702217.1"
                     /translation="MVIRYIRPVALSIALIILASISYLLATSLVLFSPSQFLQVAYLF
                     SIMVGMPVGFILFPSMLTKRYQLCQELSEVKFSWKSFVLLAIAIFLINFWCIQSDEYV
                     NQFIIATCEEFLFRYLIYRILKSEYPTWLAMLVTSLLFGVLLHMNYPLLDNLIIRTPL
                     GLLFSVLATRFGLQYAIGGHWIYNLLVSRFPF"
     gene            complement(4439..5167)
                     /locus_tag="AC1PNG_001733"
     CDS             complement(4439..5167)
                     /locus_tag="AC1PNG_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2705 family protein"
                     /protein_id="HIP6702218.1"
                     /translation="MNMFWSIFKSVIHRRDVALFYAFAGLPILVPILSKFLVGVKAEY
                     TDNFLDFLGAALATQDGIVLPILLLSLIISAVFRDEIDSGILFLYKDLNRTRLFNAKI
                     ISLVTMYASYVLLTMLTSAIAYFGFLNASGKVVSDDWNNVQSTFLSIFATISINVIGI
                     LLVAMVSIKAKSLQAVLAGVFWSLFTTTAPFLIGVRYVVPNGYAQMSFNQPLLAWSLV
                     AAITTLYIVATYLKGRSNFEKLEF"
BASE COUNT         1808 a         1185 c          879 g         1435 t
ORIGIN      
        1 attattaggt aaccctagac ttagactagt cggtccatcg actcccattc actaactggc
       61 ttgcggtaag cctcttaaga aatcttgata gagatgattt ctctccacta actgctggtg
      121 aggaccataa tcggctactt gcccattttt atctaagacc agaatcgcat ccgcctcacg
      181 cactgtgtgc aaacggtgag caatggttag ccttgttttc ccagctgtta aacggtcaat
      241 cgctttgcta atgacacgtt ctgtgacagt atctaaactt gctgtcgcct catccaacaa
      301 atagatagga tgctctaaca acaaggctct cgcaatgcta aatcgttgtt tttggccttc
      361 cgacaaacct gtcccatttt ctgaaatccg agtatctaaa ccatcttcca actcagctac
      421 taaatgagac aatcccaact ccttgagaat atgagcaata cgctcttcag acactgactc
      481 gttctttcca tacaatagat tttctctaat cgtcccatgg aagagggtat tatcttgagg
      541 tataaaggaa atcaatcttc tcatctcctt ggttgaaata tcacttaagc attgttgacc
      601 aatccgaatc tctccttggt agtcttgata gtatttcatc agcaaggcaa aaatagttga
      661 ttttcctgct ccacttggac caacaatcgc cacatgagct ccttcaggaa ttctaaccga
      721 tactccattc aaaaccggat ctgtatgata ggaaaaccga acattttcca actggatact
      781 agcttcatca accaactctg tatctgcctg ttcttcctta gtcagctcca gcaactctac
      841 caaacgttta gacaccgcct gaaactccgt tagttcattt aaggaagagg caatatctgt
      901 tactggatca atcagttgca tcatgtacaa tacaaaggtt gtcaatgtag ccatggtcat
      961 cactcccttg gtcacttcta tacctgctac cagcaacatc agaatgataa aacccgtcat
     1021 caagctacta accaaagtat gttgcaccgc tgacagtcta atcattctct caaaggtttg
     1081 cgacagtttc tgaaaagact tgcccatctc tcgttgttca tgcttttctc cctgaaaggc
     1141 cttcatcaaa cgaatcgttg taaatttatg actaattcta ctagaaacct gccctaatcc
     1201 ctcttgataa gcagagtagt aaccttccaa ccgctgattc attggaatca taaccattga
     1261 caacatagga agcaacaaga gactaagacc tgtgagccac ggactgatgg tccacagaaa
     1321 ataaattgac ccgataacca tcaaaccatt catcaagaaa tttggaagaa taacggtgat
     1381 gaagttcaga acaaccgaca tatcattggt caaacgactg gcaagatcac cggattggta
     1441 ctgatccaag tcctttacat atgtggtcaa caaggattca taaatatatt gttgcaactg
     1501 tttgagtgcg ttaccaccac tacgcccgat gaaataattg gatagggctt gtaccgtata
     1561 aaccgataca aagaaaagga tgaagccagc cagcaattct ggtcgtgtta atagattgct
     1621 taaataggtt tgatctagca actgaccaat aaactgggga aggaataagc ccattccaac
     1681 cccaagagca ctcagcgcta ttccaaacaa gaacatcgtt ttattgagcg atttcaacaa
     1741 tactcctacg agtcttctat tcatacctag aactccaaat tcttgaattt tttcgcagca
     1801 attttagtaa atacgagtgc gtaaacaagc gtcacaccaa tagctcctaa gtaagcaagt
     1861 cccactcctg ttagaccttc gctcgctaaa ggcatataac tagttggaaa aatcatcgca
     1921 actggaccgc caacaactgc taaaatcatc atcgtaatcg tcaccaagaa tcctatgatc
     1981 attgtggtaa tatttttcag atacaaacaa agagcagttg ccaaactaac cgataaaata
     2041 tcttttagta aataagcgac aatacaaaac acattactca aactctctgc caaactagta
     2101 tcaaaaacgg acggactgcc aaaaggcagg tgaattactc gaacataatg tacaaatgca
     2161 gatgtcgcaa agaagagacc atagaaaata acaattgttg ctaagagacc caaatattta
     2221 gaccagaaaa tctgtttccg cccaagatct ctgtataaaa acatggtatg ttcatctact
     2281 tccttcctaa agacagagat agccaagaaa tacagggtca aactaggaag aataaaacta
     2341 tccacagagc tcatcatcat attcgtaaag gctacaaggc taaccgaccc ctcactagca
     2401 tgaatttgca taaaattcga cccttcaata aatgaagtga ccagataaat aaaaggaaac
     2461 aaagtgaaca ggagataaat ctgcgtctcc tttcgcttcc aaatcgtttt aaagatagct
     2521 ggaaaaattt gtttcatact cactaccctt tcgtaaagat ttctttcaag tgattctctt
     2581 tcacttctag tttcacaatc aattcttgac gagccagcac accaaaaatc tgatttagct
     2641 cactggtagg tgtagcagtg gaaatttcta acacttcccc ctctagcacc acatggtcat
     2701 tgagcaaagc ctctacagag tctaggtttt tagtcgtatt caactgcacg agcacacttg
     2761 gatgagattt gccttcaaaa gcctctgcca attctcccga ttcgatatag acatagcggc
     2821 tgcacaaggc ctccagctcc cccagttggt gactcgaaat cagcatggaa atttgtcgct
     2881 cactcgacca ctgcttcaaa atatcaatca atttctgaac accaatcggg tccaagccca
     2941 caaacggctc atctaagata aggaaatctg gctccgcaac caaggcaatt gccaaagcag
     3001 tccgttgttt cataccaaac gagaatccct tgggtttccg attgcgagct tcccacaatt
     3061 ctactaattt caaagtcttc tcaatatttg gatagaggtc ttttttccca tgcagatcca
     3121 aataaaactt aagattgtcc ataactgtca tttctggata gaagacggga tcaatcataa
     3181 tcccaaaatc tgccaacaaa ttatcctgac taaaaatatc cgtcccctta taagtaatgg
     3241 ttcctgaact tggttttagg gacttggcta ttaatttcat gagcgtggat tttccagctc
     3301 cgtttttccc aatcagccca acaatctcgc ccttattgat ctctaaactc acatctttta
     3361 gtgagtaaaa ttcgtttcct gcaaactgtt ttgataaatg attgatttta agcataagta
     3421 actccttttt ctttctgacg acttgtttgt tgaataaagt atgtagcaat agctacacca
     3481 taatacacta cagcaacaaa tagtatttgt gatggagcta acagattact agctacctcg
     3541 acactcatga catcgtctga aactgtattc agttgagaaa actgagccca ctgaccatta
     3601 ctactcgtaa tgtaggcaaa agcagcataa aatatcattg acattgttgg aaaaatgcct
     3661 gctgaccatg ctttccaaga attatccttt agcaatccta aacagattga attgacaatg
     3721 ggtggtagca aataggtcaa caataacaaa ataggaaccg aaaggcttaa cagcagttgc
     3781 gaacttacaa ctgtcaaaca agtatacaat aaactcaata gtagaataaa tatcatcata
     3841 accctcacaa ttaaaatgga aatctcgaaa ccaacagatt gtagatccaa tgtcctccga
     3901 tagcatactg caatccaaaa cgtgtagcta acacagagaa caataaacct agtggagttc
     3961 gaatgattag attatccaac aatggataat tcatatgtag caaaactcca aacaagagag
     4021 aagtgaccaa catagctagc caagtagggt attcactttt cagaatacgg tagataagat
     4081 aacggaacaa gaattcttca caagtcgcaa taataaattg attgacatac tcatcacttt
     4141 gaatacacca gaaattgatc aggaaaattg caatagccag caaaacaaaa cttttccaac
     4201 tgaactttac ttccgatagc tcctggcaaa gttgataacg cttggttaac atagatggaa
     4261 atagaataaa acctactggc attccgacca taatggaaaa taggtaagcc acctgtaaaa
     4321 attgactggg actaaacaaa accaaagatg ttgccagaag atatgaaatt gacgctaaaa
     4381 taatcaaagc aattgataag gcaactggtc taatatagcg tattaccata aaacacctct
     4441 aaaattctag cttttcaaag ttcgaacggc ctttgagata ggtagcaaca atatataatg
     4501 ttgttatagc tgctaccaag gaccatgcaa gcagtggctg attaaagctc atctgagcat
     4561 agccattcgg tacgacataa cgaactccaa tgagaaatgg tgcagttgtt gtaaacagcg
     4621 accaaaaaac tcctgctaaa actgcctgta aggatttagc tttgatagaa accatggcaa
     4681 ccaatagaat accgataaca ttgattgaaa ttgttgcaaa aatagatagg aaggtggact
     4741 gtacattatt ccaatcatcc gatactactt ttccagaagc attcaagaag ccaaaataag
     4801 caatcgcact tgtcagcatt gttagaagga cataagaggc atacatcgtc actaaactaa
     4861 tgatttttgc attaaaaagt cgagttctat tcagatcttt atataaaaat agaataccgc
     4921 tatcaatctc atccctaaaa actgcggaaa ttatcaagga taacaacaat attggtaaca
     4981 caatcccatc ctgtgttgcc aatgctgctc ctagaaaatc taaaaaatta tccgtatatt
     5041 cagcctttac gcctaccaaa aatttggaca gtataggaac taagattggt aaaccagcaa
     5101 aagcatagaa caaagcaaca tcccttcgat gtattaccga tttaaaaatt gaccaaaaca
     5161 tattcatatt gtttcccctt tttatcaata agatagatga gcaataactc cctcacttat
     5221 ttattcgtaa acaaaaagaa aaattctctg gaaaaaccaa agaattttaa tacccatatt
     5281 tcaccatcaa ttatggtctc aaataat
//
LOCUS       DBNFEG010000147         5289 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.147, whole genome shotgun sequence.
ACCESSION   DBNFEG010000147 DBNFEG010000000
VERSION     DBNFEG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.147"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(19..252)
                     /locus_tag="AC1PNG_001734"
     CDS             complement(19..252)
                     /locus_tag="AC1PNG_001734"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702219.1"
                     /translation="MYLRFVLIDISDDGFYHYEIYPENKEEHKQTLVFNPETKDIRVN
                     TFDDASMKYLGKFLQNFKNQDGTYRKELSFGWG"
     gene            complement(257..1477)
                     /locus_tag="AC1PNG_001735"
     CDS             complement(257..1477)
                     /locus_tag="AC1PNG_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702220.1"
                     /translation="MNDDILPSLLKEVQDKFEASYGKSDIISSAFAKLKNKKATYSTA
                     NDFAIELGDILAEALGSSVAGDRLPDGKMYYNIGQRLLNDVLGKNFELVSDFTGQVQA
                     NLNKEANIGLKVQVPKFNQDRVDGLVNRLSSEVDFEKVSWLLKEPIVNFTQSIVDDSI
                     KTNADFHAKAGLTPKVVRKESGNCCKWCRAVVGVYNYPDVPKDVWRRHNRCRCTVDYH
                     PGNGKKQNAHSKRWSDPLKNAKIEERKGIGLLVQAGAKNYVRDDSNDELLPKEFIKAE
                     KHAYLTYDRIKNSNQDLEKRKIYSNIWKFKEMNGFSKDDVDKAFDHVFNNVHELNYGK
                     GLFAPDIDMAQSWDRLISGKNIQPHDLILLKHERLEHDYMYVSSKLDYDTAHKKVDEL
                     FNYSEAVDEFKKKG"
     gene            complement(1470..2735)
                     /locus_tag="AC1PNG_001736"
     CDS             complement(1470..2735)
                     /locus_tag="AC1PNG_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HIP6702221.1"
                     /translation="MSYKGMSYLRKKLALYQLGVKKRYRFYAMADRDNTKSFIIPDNV
                     KSMYNSVMGWTAHSVDALADRIIFREFANDDFEATEIFDANNPDIFFDTAIQSALIAS
                     CCFVYLVPNPDGLPKMQVIEASKATGVIDTTTFLLTEGYAILEVDENDNPVLEAYFTK
                     DVTWYYPKGANPYSISNPTGQPLLVPIIHRPDAVRPFGRSRITRAGMYNQLAAKRTLE
                     RAEVTAEFYSFPQKYVLGTSQDADPMEKWKTTVSSLLEITKDDDGDSPNVGQFNTASM
                     APFIDQLRMYASLFAGGSGLTMDDLGFPSDNPSSVEAIKAAHENLRAAGRKAQRSIAS
                     GLLNVAYVAVCLRDRYQYTREQFLNTKIKWEPLFEADANMLTMIGDGAIKLNQALPGY
                     INAETIRDLTGIAGDMSATPKLAEVVANE"
     gene            complement(2732..3094)
                     /locus_tag="AC1PNG_001737"
     CDS             complement(2732..3094)
                     /locus_tag="AC1PNG_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702222.1"
                     /translation="MIKKSCAFCGRSFTAESKRVKYCSDKCRKDGARDKQRKLMKQKR
                     ANLKKQKSKKDNPNNQPAKKRKKKKNLLKYYQDFKTKILANEAEFGFTSRTIVEGVEV
                     HEPDFEEQVINKIKEQPK"
     gene            complement(3183..3530)
                     /locus_tag="AC1PNG_001738"
     CDS             complement(3183..3530)
                     /locus_tag="AC1PNG_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIP6702223.1"
                     /translation="MRADKSGTHRVAFEKNKKRILKTATHCGICGQLVDKKLKYPNPM
                     CAVIDHVVPLAKGGHPSLIENLQLAHMSCNRIKSDKLFADNSKAEQKTLGNRNLPQSR
                     DWSQFISVKSEGG"
     gene            complement(3627..4061)
                     /locus_tag="AC1PNG_001739"
     CDS             complement(3627..4061)
                     /locus_tag="AC1PNG_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HIP6702224.1"
                     /translation="MNKAKAILKDLRNLDLYIASLIRRREKIEASLLSSPKWTADKVS
                     GGAKKKQDDVYVELMATADDIEAKTVEAIKKQRELQSMIDGLSNSDSQTVLSMVYIDK
                     MSPWQVMDALNCSESTYYRILRVATRELNEMTVNDRDLQKIQ"
     gene            complement(4134..4448)
                     /locus_tag="AC1PNG_001740"
     CDS             complement(4134..4448)
                     /locus_tag="AC1PNG_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702225.1"
                     /translation="MTLFDEVQQLSSESYDKWFERYFKKYDLEKVIKKSAMQGYSGHL
                     ISVLKVKDDYTRRRLDDERTLEKIKELLGDGFKVEYHSTYSKNLFTGEDFITNKQIHI
                     SW"
     gene            complement(4637..4852)
                     /locus_tag="AC1PNG_001741"
     CDS             complement(4637..4852)
                     /locus_tag="AC1PNG_001741"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702226.1"
                     /translation="MTKTQEPCLAKIGKYWERADFLGVFQHSGTNLIFGHQFAGPVAV
                     VRFRGRLVKVEIENIDFCEVENEQAAT"
     gene            complement(4854..5162)
                     /locus_tag="AC1PNG_001742"
     CDS             complement(4854..5162)
                     /locus_tag="AC1PNG_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HIP6702227.1"
                     /translation="MKEIDPLVLVTLCIVIALFAAVTEVKVLREQVKRLEEREMVIIH
                     KVDNAGVTMVGKVTRKDIIDGRYYVEIGAYGKFLVTKGQFETINIGDDIPDYLQGRGS
                     "
     gene            complement(5159..5281)
                     /locus_tag="AC1PNG_001743"
     CDS             complement(5159..5281)
                     /locus_tag="AC1PNG_001743"
                     /inference="COORDINATES: protein motif:HMM:NF048218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7204 family protein"
                     /protein_id="HIP6702228.1"
                     /translation="MLVDETHYFENEADALKCKAGLEARYRGQRLYSVKMEEVK"
BASE COUNT         1436 a         1215 c          862 g         1776 t
ORIGIN      
        1 actcgatatc tcgaatgctt atccccaccc aaacgacaac tctttacgat aggtcccgtc
       61 ttggttttta aaattttgta aaaatttccc taaatacttc atgctcgcat cgtcgaatgt
      121 atttacacga atatcttttg tttcaggatt aaaaacaagc gtttgcttat gctcctcctt
      181 attttcaggg taaatctcat agtgataaaa accatcgtca ctaatatcta tcaaaacaaa
      241 tcttaaatac atacctttac cctttctttt taaattcgtc gacagcttcg gagtaattaa
      301 ataattcatc aacttttttg tgagctgtat cataatccaa cttgctactc acatacatat
      361 aatcatgttc taagcgttca tgcttcagca aaattaaatc atgtggttga atgttttttc
      421 cagaaatcaa tctatcccag gattgagcca tgtcaatgtc tggtgcaaac aaccccttcc
      481 cataattcaa ctcatgaaca ttattaaaca catgatcgaa cgctttatca acatcatctt
      541 ttgaaaagcc attcatttct ttgaacttcc aaatgttcga atagatttta cgtttttcca
      601 aatcttgatt gctgtttttt atcctgtcgt aggttaaata agcatgtttt tcggctttaa
      661 taaattcctt tggcaaaagc tcgtcgtttg aatcatcacg aacataattc tttgcgccag
      721 cttgaacaag tagtccgatg cctttccttt cctctatttt agcatttttt aaaggatcag
      781 accaacgttt tgagtgtgca ttctgttttt tgccgtttcc tgggtgataa tcaacagtac
      841 acctgcaccg attatgcctc ctccaaacat cctttggaac atctggatag ttataaaccc
      901 caacaacagc cctacaccat ttacaacaat taccactttc ttttctgaca actttaggtg
      961 tcaaccctgc cttggcatga aaatccgcat tcgttttgat actatcatcc acaattgact
     1021 gggtaaaatt gacaatcggt tctttcagca accacgacac tttttcaaaa tcaacttcag
     1081 atgacaagcg attgaccaag ccatcaactc tatcttgatt gaatttcgga acttggactt
     1141 ttagaccgat attagcttcc ttgttcaagt tcgcttggac ttgtcctgta aagtcgctca
     1201 caagctcaaa attttttcct agcacgtcat tcagtaaacg ttggccgata ttgtaataca
     1261 tttttccatc tggcaaccta tcgcccgcaa cagatgagcc aagagcttct gctaagatgt
     1321 ctccaagctc aatggcaaaa tcatttgctg tggaataggt tgcctttttg tttttgagct
     1381 tggcaaaagc agagctgata atgtcacttt ttccatagga agcttcaaac ttatcctgaa
     1441 cttctttcag taagctaggt aagatatcat cattcatttg ctaccacctc cgctaacttc
     1501 ggagtggccg acatatcacc agcaatacca gtcaaatccc gaatggtttc ggcgttgata
     1561 tagccaggta aggcttgatt aagtttgata gctccatcac caatcatggt cagcatgttg
     1621 gcgtcagctt caaagagcgg ttcccacttg atttttgtat tgagaaactg ctctctggtg
     1681 tattggtatc tatcccgtaa acaaacggcc acataggcta catttaacag accagatgcg
     1741 attgagcgtt gggcttttcg ccctgcagcc ctcaaattct cgtgcgccgc cttgatagct
     1801 tcgacagatg atggattatc cgatggaaaa ccaaggtcat ccatggtcag accgcttcca
     1861 ccggcaaata atgatgcata cattcgcaac tgatcaataa atggtgccat gctagcagtg
     1921 ttaaactgac cgacattagg actatctccg tcgtcgtcct ttgtaatttc aaggagactc
     1981 gacactgtcg ttttccactt ctccatcggg tcagcatctt gactagttcc cagaacatac
     2041 ttttgcggga acgagtaaaa ttctgctgtc acttctgcac gctctagggt tcgcttagct
     2101 gccaactgat tgtacatgcc tgccctagta attcgactgc gaccaaaggg acggaccgca
     2161 tcaggtctgt gaataattgg cactagtaaa ggttgaccgg tcggattgga gatactgtat
     2221 ggatttgcac ctttagggta ataccatgtc acatctttgg taaaatatgc ctctaatacc
     2281 gggttgtcgt tttcgtccac ttctaggatt gcatatccct cagtcagcaa aaatgttgtg
     2341 gtgtcaatga caccggttgc cttgctggct tcaatgacct gcattttagg caagccatca
     2401 ggatttggga ccaagtacac aaaacaacaa gatgcaatca aggctgattg aatagccgtg
     2461 tcaaaaaaga tatctgggtt gttagcatca aagatttcgg ttgcttcaaa atcatcattt
     2521 gcaaactcac ggaaaatgat tctgtcagcc aaggcatcca cactgtgagc cgtccatccc
     2581 ataaccgagt tatacatgct tttgacattg tcagggatga taaaactttt ggtattatct
     2641 ctgtctgcca tagcatagaa cctataacgt ttcttaactc ctaattgata tagagctaac
     2701 ttcttccgga gataactcat tcctttgtaa ctcattttgg ctgctcctta attttattga
     2761 tcacttgctc ttcaaagtct ggttcgtgga cttccacacc ttcgacaatg gtccgactag
     2821 taaaaccaaa ctcagcttca ttggccaaaa tcttggtttt aaaatcttga tagtatttca
     2881 gcaaattttt ctttttttta cgttttttgg ctggttggtt gttaggatta tcttttttag
     2941 atttttgctt tttgaggtta gcgcgttttt gtttcatcaa tttacgctgt ttgtctctag
     3001 ccccgtcctt acggcattta tcactgcaat atttaacccg ttttgattct gccgtgaagc
     3061 ttcgtccaca aaaagcacat gattttttta tcatttttga taactcctat accacttaat
     3121 cctaacgcga aaaaaaatgt acagtgacgg cgtgaagggc cgccgagcga gttggtgggg
     3181 ttctaccccc cttcagattt tacagagata aactgtgacc aatcgcgact ctgaggaaga
     3241 tttctatttc ccaatgtttt ttgttctgct ttagaattat ctgcgaataa cttatcagat
     3301 ttaattctat tgcaagacat gtgagccaac tgcagatttt caattaacga tggatgaccg
     3361 ccttttgcta atggaaccac atgatcgatt actgcacaca ttggatttgg atattttaat
     3421 ttcttatcaa ctaactgacc acatattcca caatgtgttg cagtcttaag tattctcttc
     3481 ttattctttt caaaagctac tctatgcgtt ccgctcttgt ctgctctcat cgttacctca
     3541 aacaaaagag gcaccgtata attacagcac ctctcaattt cttgatgata taataatagc
     3601 acgttgaaac tgtcatgcac tgtcaattac tgtatttttt gcaaatctct gtcatttact
     3661 gtcatttcgt taagttctct tgttgctaca cgtaggatac ggtaataggt ggactcactg
     3721 caattaaggg catccattac ttgccagggg ctcattttat ctatatagac cattgagagt
     3781 accgtttgag agtctgaatt actaagacca tcaatcatac tctgtagttc gcgctgtttt
     3841 ttaattgctt cgacagtctt tgcttcaata tcatctgctg ttgccatcaa ttcaacgtag
     3901 acatcatcct gcttcttttt cgctccgccc gaaactttat ccgctgtcca ctttggacta
     3961 gatagtaagg aggcttcgat tttttcacga cgtctaatca agctagcgat gtacaaatct
     4021 aaatttctca aatcctttaa tatagcctta gctttgttca ctctctttct cctttgtggt
     4081 ataatattga tagcgaaacc acagcctggg cagagagtgc cttggctttt ttgttaccat
     4141 gaaatatgaa tttgcttgtt agtgataaaa tcttcaccag taaacaaatt tttactatag
     4201 gtcgaatgat actctacttt gaaaccatct cctaacaatt ccttgatttt ctcaagggtc
     4261 ctttcatcat ccaatcttcg tctcgtataa tcatctttaa cttttaagac actaatcaga
     4321 tggccactat acccttgcat agctgatttc ttaatgactt tttccaaatc atatttttta
     4381 aagtaacgtt caaaccactt gtcatagctt tctgaactta attgctgtac ttcatcaaat
     4441 agtgtcattt caatcccatc ctttcattat tcagttttat aatcttgaaa taccatagta
     4501 ttcatagcca caatattcag agcaaaaacc gtatgtatta aaatatttgt caaatactcc
     4561 gattttttct ttacaaacag ggcaatgcgt tctacggtat ctttcttctg tactcaatcc
     4621 attcagtatt ttctttttac gttgccgctt gttcattttc cacctcacaa aaatcaatat
     4681 tctcaatctc tacttttacc agtctacctc taaatctaac tacagcaact ggtccagcaa
     4741 attgatgacc aaagataaga tttgtcccag aatgttgaaa cactcccaaa aaatcggctc
     4801 tttcccagta tttacctatt tttgccaagc aaggctcttg agtttttgtc atctcaacta
     4861 ccccttcctt gtaggtaatc cggaatatca tcaccaatat taatcgtttc aaactgaccc
     4921 tttgtgacta ggaatttgcc gtaggcaccg atttctacat agtacctgcc gtcaataata
     4981 tccttccttg tgacttttcc gaccatagtc acgcccgcat tatcgacttt atggataata
     5041 accatttcac gttcttccag tcttttcact tgttcacgca atacttttac ttctgtcact
     5101 gctgcaaata aagcgatgac aatacacaga gtcaccaaaa ccaatggatc aatttctttc
     5161 atttaacttc ctccatcttc acactgtaca accgctgacc tcgataccta gcttccagcc
     5221 ctgctttaca tttcaaagca tcagcttcat tttcaaagta atgcgtttca tcaactaaca
     5281 tgtgatcaa
//
LOCUS       DBNFEG010000148         5211 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.148, whole genome shotgun sequence.
ACCESSION   DBNFEG010000148 DBNFEG010000000
VERSION     DBNFEG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.148"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..316
                     /locus_tag="AC1PNG_001744"
     CDS             <1..316
                     /locus_tag="AC1PNG_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIP6702229.1"
                     /translation="MSGTQSSRNVMIISQKSEEIKDYITKVADRGVTELPIIGGFTGV
                     DKRMLMTTISIPEMQKLETAVLEIDETAFMVVMPASQVRGRGFSLQKDHKHYDEDILI
                     PM"
     gene            381..2003
                     /locus_tag="AC1PNG_001745"
     CDS             381..2003
                     /locus_tag="AC1PNG_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIP6702230.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPSTGHISLGPDERLSVLRQNHFDYEDERVIDVVIMGNEQLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNISEDLHYQNMSELTNGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSINWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKIFVGNYDFWKQSSELAAKLQADRNAKAEEKIKELQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIIPSSRKYPFINFKSEREIGNDLLTVENLKVVIDGETI
                     LDNISFILRPGDKTALIGQNDIQTTALIRALMGDIEYEGTIKWGVTTSQSYLPKDNTR
                     DFDTNESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKAFKESIIFASHDHEFIQTLANHIIVI
                     SKNGVIDRIDETYDEFLENAEVQAKVQELWKA"
     gene            2310..4889
                     /locus_tag="AC1PNG_001746"
     CDS             2310..4889
                     /locus_tag="AC1PNG_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIP6702231.1"
                     /translation="MKKIFTKTSIYYLLSFLIPLTIISIVLAFQGIWWGSDTTILASD
                     GFHQYVIFNQTLRNTLHGDGSLFYTFSSGLGLNFYALSSYYLGSFLSPIVFFFDLQSM
                     PDAIYLVTIVKFGLTGLSTYFSLKGIHKNLKEEWALLLATSFSLMSFSTSQLEINNWL
                     DVFILLPLVLLGLHRLLKKQGPILYYITLTCLFIQNYYFGYMVAIFLTLWTLVQLSWI
                     DSQRIKRFIKFTIVSILSALSSMFMLLPTYLDLKTHGETFTKIVNLKTEDSWYLDFFA
                     KNLVGSFDTTKFGSIPMISVGLVPLILALLFFTLKEIKPTIKLSYALFFTFIISSFYL
                     QPLNLFWQGMHAPNMFLYRYAWALSITVIYLAAETLVRLRQVSIKNFTLIVSFLLICF
                     TSTFIFRDHYEFLTDVNFLLTLEFLIAYFILFVAMIRYKSSLKWINIVLLFFTFLELG
                     LHSHYQVQGISDEWHFPSRSNYEEKLTDIDSIVKSTKTTTDSFYRIERLLPQTGNDSM
                     KFNYNGISQFSSIRNRASSSALDKLGFRSDGTNLNLRYQNNTIIADSLFGVKYNLATT
                     DPNKFGFTLNQSQSTINLYENSFNLGLALLTEGIYKDVNFTNLTLDNQTNFLNQLTGL
                     SQKYYHTLSDVISQNTVELSNRMTVNKVDNEDAAKATFLVNIPANSQVYLNLPNLTFS
                     NENQKKVVITVNNQSSEFTLDNAFSFFNVGSFTTDVQVQVDVYFPENNQVSFDKPQFY
                     RLDLLAFQQAISILQEKQVVTKTDGNKVTVDFVTDKEASLLLTLPYDKGWNATIDGKP
                     IKIQKVQEGFMKVDVSPGQTKLVLTFVPNGFYLGLLISFGAVFVFFSYQFIGYYYSKN
                     REY"
     gene            complement(4958..5030)
                     /locus_tag="AC1PNG_001747"
     tRNA            complement(4958..5030)
                     /locus_tag="AC1PNG_001747"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4995..4997),aa:Asn,seq:gtt)
     gene            complement(5036..5107)
                     /locus_tag="AC1PNG_001748"
     tRNA            complement(5036..5107)
                     /locus_tag="AC1PNG_001748"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5072..5074),aa:Glu,seq:ttc)
BASE COUNT         1605 a         1023 c          913 g         1670 t
ORIGIN      
        1 gatgtctggt acccaatctt ctcgaaatgt catgattatt tctcaaaaat cagaagaaat
       61 taaagactat attactaaag ttgctgatcg cggtgtgaca gaacttccaa ttattggtgg
      121 ctttacaggg gttgacaagc gcatgttgat gactaccatc tctattccag aaatgcagaa
      181 actggagaca gcggtattag aaattgatga aactgccttt atggttgtta tgcctgcgag
      241 tcaggtgcgt ggacgtggct tcagtcttca aaaagaccat aaacattatg atgaagatat
      301 tttgattcca atgtaattca ttgaaaattc aagttctttc tagtacaata aaactaatag
      361 acagaaatag aggagaaact atgcttacag tatcagacgt gtcgcttcgc tttagcgacc
      421 gcaaattatt tgacgatgtc aatatcaaat ttacagcagg aaatacctac ggtttgattg
      481 gtgccaatgg tgctggcaaa tctacatttt tgaaaattct tgctggtgac atcgagccat
      541 caacaggtca tatttcactt ggaccagacg aacgcctttc tgtactacgt cagaaccatt
      601 tcgactatga agatgagcgc gtgattgacg ttgttatcat gggaaatgag caactttaca
      661 gcatcatgaa agaaaaagat gctatttaca tgaaggaaga tttttctgat gaagatggtg
      721 ttcgtgctgc tgaattagaa ggtgagtttg ctgaacttgg tggctgggaa gctgaaagtg
      781 aagcttctca attgctccaa aacctcaaca tttcagaaga cctccactac caaaacatga
      841 gcgaattgac caacggtgag aaggtcaagg tcctcttggc caaggccctc tttggtaagc
      901 ctgatgttct tcttttggac gagccaacca acggtctgga catccaatct atcaactggc
      961 tcgaagattt cttgattgac tttgaaaata cggttatcgt cgtgtcacac gaccgccact
     1021 tcttaaacaa agtatgtacc catatggccg accttgactt cggtaaaatc aagattttcg
     1081 ttggtaacta tgacttctgg aaacaatcca gtgaactagc agctaaacta caagctgacc
     1141 gtaatgccaa agcagaagaa aaaatcaaag aactacaaga attcgttgcc cgtttctctg
     1201 ctaatgcctc taagtctaag caagctactt cacggaaaaa gatgctggac aaaatcgagt
     1261 tggaggaaat tattccttct agccgtaagt atccatttat caacttcaaa tcagaacgtg
     1321 aaattggtaa tgacctccta acagttgaaa acttgaaagt tgtcatcgat ggtgaaacga
     1381 ttcttgacaa tatcagcttt atcctgcgtc caggtgacaa gactgctctt attggtcaaa
     1441 acgacatcca aacaactgct ctcattcgtg ctcttatggg cgatattgaa tatgaaggta
     1501 ctatcaagtg gggtgtcact actagtcaat cctacttacc aaaagacaat actcgtgact
     1561 ttgatacaaa cgaatctatc cttgattggc tccgtcaatt tgccagcaag gaagaagatg
     1621 acaatacctt cttgcgcggt ttcttgggac gtatgctctt ctcgggtgat gaggttaaca
     1681 aacctgtaaa cgtcttgtca gggggcgaaa aagtgcgcgt gatgttgtca aaactcatgc
     1741 tcctcaagtc aaatgtcttg gtcttagatg atccaaccaa ccacttggac ttggaatcaa
     1801 tttccagtct gaatgatggt ttgaaagctt ttaaagagtc catcatcttt gccagccacg
     1861 accacgaatt tattcaaact ttagcaaacc atatcatcgt catttctaaa aacggtgtta
     1921 tcgaccgaat cgacgaaact tatgatgaat tcttggaaaa tgctgaagta caagcaaaag
     1981 tacaagaact ttggaaagca taataaaaag aggctgggac ttttgcccag cctctcttct
     2041 cagagttcgt gtcaacatct cagcgcagtg gttgattgcc agatttgttc gtgtttcaca
     2101 ctccaaatct ggccattacg actgttgcga acaaagttcg ccttatctcc aacctcaaac
     2161 tgtctcccag acagtttgag ctgtgcgggg gtgggagtga aacagtctgg gaatagactg
     2221 tttcagctca acaactagaa ataaagactt gttgacgaac tcttttttga ccggtcaagt
     2281 tctttcccac ttccttttct tttaaatcta tgaaaaaaat atttacaaaa acatccattt
     2341 attatctctt atctttcctt attccgctga ctattatttc tatcgtctta gcatttcaag
     2401 gaatctggtg gggaagtgat acaacaatat tggccagtga tggtttccat cagtatgtta
     2461 tttttaatca aacattacga aatactttac acggagacgg ctctttattt tacacatttt
     2521 caagtggttt aggactcaat ttttatgctc tatcctccta ttacctaggt tcattcctat
     2581 ctccaattgt ctttttcttt gatttacaat ccatgcctga cgctatctac cttgtcacta
     2641 ttgtcaaatt tggattgact gggttgtcaa cttatttcag tctaaagggt atccataaaa
     2701 atttgaaaga agaatgggca ctattactcg ctactagttt ttctctgatg agttttagta
     2761 caagtcaatt agaaataaac aactggctag acgtttttat acttctccca ctcgttcttc
     2821 ttggattgca tcgattatta aaaaaacagg gtccgattct ctactacata acattaacat
     2881 gcttgtttat ccaaaactat tacttcggtt atatggtggc tattttccta acattatgga
     2941 cattggttca attatcatgg atagatagtc agagaatcaa aagatttatc aagtttacaa
     3001 tagtgtcaat tttatctgct ctaagcagca tgttcatgtt gttaccaacc tacttggatt
     3061 taaaaactca tggtgagaca tttacaaaaa ttgtcaacct aaaaaccgaa gactcttggt
     3121 atctggactt ctttgcaaaa aacttagttg gtagctttga tacaacaaaa tttggttcta
     3181 ttcccatgat ctctgttggt ctcgttccat taatactcgc tttactattc tttacattaa
     3241 aagaaataaa accaactatc aaactctcct atgctctatt ctttacattc attatttcaa
     3301 gtttctacct acaaccactc aatctttttt ggcaaggtat gcatgcgcca aacatgttcc
     3361 tctatcgtta tgcatgggct ctatcaataa ccgttatcta tttagcagca gagacattgg
     3421 tacgactacg tcaagtaagt attaaaaatt ttactctgat tgtttcattt ttactcattt
     3481 gctttacttc tacctttatt tttagagatc attatgagtt tctaacggat gtaaatttcc
     3541 tactcacact agaatttcta atagcctact tcattctttt tgtagcaatg attcgctata
     3601 aatcatcctt aaaatggatt aatattgttt tattgttctt cactttctta gaacttggat
     3661 tacatagtca ttatcaagtt caggggattt ctgatgaatg gcattttcct agccgatcaa
     3721 actatgaaga aaaattgaca gatattgaca gtattgtaaa gtctacaaag actacaactg
     3781 attcatttta tcgtatagaa cgtctattac cacaaactgg caatgatagc atgaaattca
     3841 attacaacgg tatttctcaa ttctcctcca ttcgaaatcg tgcttccagc tcagcactag
     3901 ataagctcgg tttccgttca gacggtacca atttgaatct tcgttatcag aataatacaa
     3961 ttattgctga tagcctattc ggagtcaaat ataatttagc tactacggac ccaaataagt
     4021 ttggtttcac actgaatcag agtcagtcta ctattaatct ttacgaaaat agttttaatc
     4081 tagggttagc cctattgact gaggggattt acaaagatgt caattttaca aatctgactc
     4141 tcgataatca aacaaatttc ctcaatcaac taacagggct atctcaaaaa tactaccaca
     4201 ctttatcaga tgttatttca caaaatacag ttgaactgag caatcgaatg actgtcaaca
     4261 aagttgacaa tgaggatgct gcaaaagcaa ctttcttagt aaatatacct gcaaatagcc
     4321 aagtatactt aaatttgcca aatttaacat tctcaaatga aaatcaaaaa aaggttgtca
     4381 ttactgtcaa taatcagtca agtgagttta cattagataa tgctttctct ttcttcaatg
     4441 tggggagctt tactacagat gtacaagttc aagtcgatgt atattttcct gaaaataatc
     4501 aagtttcttt tgataaacca caattttatc gattggactt attagctttc caacaggcta
     4561 tttccattct ccaagaaaaa caagtagtaa caaaaactga tggtaataaa gtaaccgttg
     4621 attttgtaac cgataaagaa gcctcactcc tccttacttt accctatgat aaaggatgga
     4681 atgcaaccat tgatggtaaa cctatcaaaa tccaaaaagt gcaagaaggt tttatgaaag
     4741 tggatgtaag tccaggtcaa actaaactgg ttttaacctt tgtaccaaat ggtttctatc
     4801 taggtttact gatttctttt ggtgcagttt ttgtattttt ctcctatcaa ttcattggat
     4861 actattattc taagaaccga gaatactaaa ctttctcggt tttttcttgt aaaaagcaaa
     4921 aaaccttgat ttctcaaggt ttcctatgct ttatctactc cgccaacagg gctcgaacct
     4981 gtgacatcat gattaacagt catgcgctct accaactgag ctatggcgga agaaatagtc
     5041 cgtacgggat tcgaacccgt gttaccgccg tgaaaaggcg gtgtcttaac cccttgacca
     5101 acggaccatt tttagaacaa taactagtat aatacatgtg actttgtttg tcaatacatt
     5161 ttttgatttt ttattgtatt gacagagtgc tttgtttaat gtaaaataaa a
//
LOCUS       DBNFEG010000149         5023 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.149, whole genome shotgun sequence.
ACCESSION   DBNFEG010000149 DBNFEG010000000
VERSION     DBNFEG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.149"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            48..797
                     /locus_tag="AC1PNG_001749"
     CDS             48..797
                     /locus_tag="AC1PNG_001749"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HIP6702232.1"
                     /translation="MQKIDLLDGERIDQLFSTDVQIIQNREVFSYSIDSVLLSRFPKM
                     PAQKGLIVDLCSGNGAVGLFASTRTKAQIMQVELQERLADMNRRSIALNGLEEQVSVI
                     NDDLANLPQYDLRSKVDLMLCNPPYFKVDKDSNLNESEHYLLARHEIATNLDSICQVA
                     QQVLKSNGRLAMVHRPDRFLDILDKLRTYKLAPKRIQFVYPKASKEANMLLIEAIKDG
                     SVDGLHILPPLVVHEENGDYTPAIREIYYGK"
     gene            787..1059
                     /locus_tag="AC1PNG_001750"
     CDS             787..1059
                     /locus_tag="AC1PNG_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIP6702233.1"
                     /translation="MENKAYFYVLECADGSLYTGYTTDVEKRLATHNRGKGSKYTRGR
                     LPVSLLYQEAFASKQEAMSAEALFKKRSRQSKLDYIAEMTKKPRTK"
     gene            complement(1075..1686)
                     /locus_tag="AC1PNG_001751"
     CDS             complement(1075..1686)
                     /locus_tag="AC1PNG_001751"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0050446 - azobenzene reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HIP6702234.1"
                     /translation="MVTVLIVKAHPLDAQKSYALRALEEFQTRYASLHPEDQIEVVDV
                     FEDQIPALDKSLLEAMGAAKKGESISPDQAKQLDRYNALTQQFLMADKIVVVNPLWNL
                     NVPSQLVSWVNTINVAGLTFKYGPEGSIGLVKDKKLLHIQSNGGVYAGQDPAAQYIKS
                     IFEFLGFEDIHQVFIEGQSADPSQAQAIFEEAMGKIDAILEVF"
     gene            complement(1809..2216)
                     /locus_tag="AC1PNG_001752"
     CDS             complement(1809..2216)
                     /locus_tag="AC1PNG_001752"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6702235.1"
                     /translation="MITYKQNPQLDFQAVLEIYDSVGWTNYTDRPTMLQKALEHSLLV
                     LAAFDGDRLVGLLRAVGDGHSIVFIQDILVLPTYQRQGIARQLLEQAIAYFPGIYQLH
                     LLTDNTEKTRSFYEAIGFTAVDSLDSVAYTYLK"
     gene            complement(2328..2930)
                     /locus_tag="AC1PNG_001753"
     CDS             complement(2328..2930)
                     /locus_tag="AC1PNG_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HIP6702236.1"
                     /translation="MKKIDIKTLFATSSIFLLIALLTIYYYGALPDTMVTHFGVNGEP
                     NGSMPKYMVTILTPLLFFCVHLFICILYDVKGIGKTPVIRVFKWLFPLLALIIQVSLL
                     WYNLGGELDYRRLVIGLLAVYYMVIGNYLPKEELDSKTNEIERKGRKQSGYLLMIGGL
                     LQLVSLLGSPTLSILVMILVGISVVSLSIYYAYLHFKTAS"
     gene            complement(2917..3195)
                     /locus_tag="AC1PNG_001754"
     CDS             complement(2917..3195)
                     /locus_tag="AC1PNG_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HIP6702237.1"
                     /translation="MGLSETLKAISAPIRRQILDSLKSGPKSAGEIVEQFQLTGATVS
                     HHLSTLKKAGLILEEKQKNFIYYRLNYTVFEEVLVWIASFGGQQDEEN"
     gene            complement(3340..4920)
                     /locus_tag="AC1PNG_001755"
     CDS             complement(3340..4920)
                     /locus_tag="AC1PNG_001755"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIP6702238.1"
                     /translation="MKFTDLQLSEDILLAVEKAGFETPSPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIDTNNQAIQALIIAPTRELAVQSQEELFKFGREKGVKVRS
                     VYGGSSIEKQIKALRSGAHIVVGTPGRLLDLIKRKALKLDGVETLILDEADEMLNMGF
                     LDDIEAIIERVPESRQTLLFSATMPEPIKRIGVKFMKEPEHVKIAAKELTNVNVDQYY
                     LRVKEHEKFDTMTRLMDVDQPELSIVFGRTKRRVDELTRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDQIDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     QSGQSITFVSPNEMGYLAIIEDLTKKRMKGLKPATAQEAFEAKKKVALKKIEREMADE
                     TIRSNFDKFKKDAIQLAAEFTPEELALYVLTLTVQDPENLPEVEIAREKPLPFKFAPG
                     QAKGKGGRGGRDRDRGGRRDGDRNRDRGGRRGDRNDRNRRDDKFKRDNRRQDNKKPHQ
                     RTSSEKKTGFVIRNKGER"
BASE COUNT         1394 a         1139 c         1028 g         1462 t
ORIGIN      
        1 tttcaaaata tacagtgatt ttcattcaaa gatggtataa tatacctatg caaaaaattg
       61 atttactaga cggagagcgg atcgaccagc tcttctcgac agatgttcag attattcaaa
      121 atcgagaggt ttttagctac tcgattgaca gcgttcttct ttcgcggttt ccaaaaatgc
      181 ctgctcagaa ggggctgatt gttgaccttt gtagcggaaa tggggccgtg ggtctctttg
      241 cctccactcg caccaaggct cagattatgc aggtcgagtt gcaggaacgc ttggcggata
      301 tgaaccgccg ctccattgcc ctcaatggtt tggaggaaca ggtctcggtt atcaatgatg
      361 acttggcaaa tctgccccag tatgatctgc ggtccaaggt tgatttgatg ctctgcaatc
      421 ctccctactt caaggtagat aaagactcaa acctcaatga aagcgagcat tatctcctag
      481 ctagacacga gattgcgacc aacctagaca gcatttgtca ggtggcccaa caggtgctca
      541 agtccaatgg tcgcttggcc atggttcaca ggcccgaccg gtttttagat attttggaca
      601 agttgagaac ctacaaattg gctcccaaac gcatccagtt tgtctatccc aaggccagca
      661 aggaggccaa tatgttgctg attgaggcta tcaaggacgg ctcggtggac ggtctgcata
      721 tcctgccacc tctggttgtc catgaggaaa atggcgacta tactccggct attcgtgaga
      781 tttattatgg aaaataaggc ctacttttat gttttggaat gtgccgacgg gagtctttat
      841 actggctata cgactgatgt ggagaagcgg cttgctaccc ataatcgtgg gaaagggtcc
      901 aagtacaccc gcggacgctt gcctgtcagc ttgctctatc aagaagcctt tgctagtaag
      961 caggaagcca tgtctgccga ggctttgttt aagaaacgca gtcggcagag taaattagat
     1021 tatattgcag aaatgacaaa aaaacctcgt acaaaatagt acgaggcttt ttatttaaaa
     1081 tacttccaaa attgcatcaa ttttccccat agcttcttca aaaatagcct gagcctggct
     1141 tggatcggca gattgccctt cgataaagac ttgatgaata tcttcaaagc ctaaaaattc
     1201 aaagatagac ttgatgtact gggctgctgg atcctgacct gcataaacac caccattgga
     1261 ctggatgtgc aggagctttt tgtccttgac caaaccgata gagccttctg gaccatactt
     1321 aaaggtcagg cctgctacat tgatggtatt gacccaagat accaattgac ttggcacatt
     1381 gagattccag agaggattga cgacaactat cttatccgcc ataagaaatt gctgggtcaa
     1441 ggcattgtaa cggtccaatt gctttgcttg atcaggactg atactctcgc cttttttagc
     1501 agctcccata gcctctaaca aagatttgtc caaggctgga atctggtctt caaaaacatc
     1561 cactacttct atctgatctt ctgggtgcaa actggcgtaa cgagtttgaa actcttccaa
     1621 agcccgcaag gcgtaggact tttgtgcgtc gagggggtgg gcctttacaa ttaatacagt
     1681 taccattgtt catctccttt gttactaatt attgataatt atattctact ctttttgatt
     1741 tgaaattcaa atcatttgct cgaagcaaaa ggctggtctc ccagcctctg cttttttaga
     1801 ttgttttatt attttaaata ggtgtaagcg acactgtcca ggctatcaac ggctgtaaag
     1861 ccaattgcct catagaaaga ccgtgttttc tcagtattgt ccgtcaagag atggagttga
     1921 taaatacctg gaaagtaggc aatggcctgc tccaaaagct gacgcgcaat tccttgtcgc
     1981 tgataggttg gtaggaccaa gatgtcctga ataaaaacga ttgaatgccc gtcgccaact
     2041 gctcgcaaga gacccactag acggtcaccg tcaaaggcag caagaacgag cagactatgt
     2101 tccaaggcct tctgtaacat ggtaggccgg tcagtataat ttgtccaacc aactgaatca
     2161 tagatttcaa ggactgcttg aaagtcgagt tgaggatttt gtttgtaagt aatcatgtct
     2221 ttctcctttg tattgttttt tagaatacaa aggctttgtc cattgttaaa acgcccatgg
     2281 tcttacctcc tgttttacta catttagtat aacaaaaact gctccaatta tgaagcggtt
     2341 ttaaaatgca gataagcata atagatacta agacttacaa cagaaatacc aaccaatatc
     2401 attacaagga tagatagggt tggtgaaccc aacaaactta ctaactgcaa caacccacca
     2461 atcatcaaga gatagcctga ctgctttctt ccctttcgtt ctatttcatt ggtcttgcta
     2521 tccaattcct ccttgggtaa ataatttcct atcaccatat aatagacagc cagcagtcca
     2581 atgacaagcc tacggtaatc caattctcct cctaaattat accagagtaa agaaacttga
     2641 atgatgagag ccagtagcgg aaaaagccat ttgaacactc gaatgaccgg tgttttccct
     2701 atccctttca catcataaag tatacaaatg aataggtgaa cacaaaagaa tagtagcggt
     2761 gtcaaaattg tcaccatata cttgggcata gagccatttg gctcaccatt tacgccaaaa
     2821 tgcgttacca tggtatctgg caatgctcca tagtaataaa tcgtcaataa agcaatcagt
     2881 aggaatatac tagaagttgc aaataaggtt tttatgtcaa ttttcttcat cttgttgccc
     2941 tccaaaactt gcaatccaaa ccaagacttc ttcaaagacc gtatagttta gacgataata
     3001 aataaaattt ttctgctttt cctccaaaat taatccagct tttttcaaag tggataaatg
     3061 atgagaaact gttgctcctg ttaattgaaa ttgttctaca atttccccag cagacttcgg
     3121 ccccgactta agactatcta gaatttgcct acgaatagga gcagaaattg ccttcaaggt
     3181 ttcagacaat cccataataa gcctccttgt ccatctattt agaaatattt ctaaatagaa
     3241 ttatactctg tttttattgt ttgtcaagaa ctatttcgat tattttctaa atagatttca
     3301 aaaaaaacag accactcttt atggtctgtc aaatccggct tatctctccc ctttattgcg
     3361 gataacaaag cctgttttct tttcacttga tgtgcgttgg tgtggttttt tgttgtcctg
     3421 acggcggttg tcgcgtttaa acttgtcatc gcgacggttg cggtcattac gatcaccacg
     3481 gcgtcctccg cggtctcggt tgcggtctcc atcacgacga ccaccgcgat cacggtcgcg
     3541 accaccacgt ccacccttgc ccttggcttg accaggtgca aacttgaatg gcagtggttt
     3601 ttctcgcgca atttctacct ctggcagatt ttctggatcc tgaacagtca aggtcaatac
     3661 ataaagcgct aactcttcag gggtaaattc agccgctaac tgaatagcat ctttcttgaa
     3721 cttatcaaaa ttgctacgga tggtttcatc cgccatttca cgctcaatct tcttcaaagc
     3781 aactttcttc ttggcttcaa atgcttcctg agcagttgct ggcttgagac ctttcatgcg
     3841 tttcttggtc aaatcttcga tgatggccaa gtagcccatc tcgtttggcg aaacaaaggt
     3901 aatagactga cctgattgac ccgcacgacc tgtacgaccg atacggtgca catagctttc
     3961 agggtcctgt ggaatatcgt agttatagac atgcgttaca cctgagatgt ccaaaccacg
     4021 ggccgcaacg tctgtcgcaa ctaagacatc aatttgatca tttttaaaat cacgaatgac
     4081 acgaagacgc ttgttctggt ccaaatctcc gtgaatacct tctgcacgga aaccacgcaa
     4141 tttcaaacca cgagtcaatt catcgacacg gcgtttggta cgaccaaaga caatagacaa
     4201 ttctggctgg tcaacatcca taagacgagt catggtgtcg aatttttcat gttctttgac
     4261 acgaagataa tattggtcaa cattgacatt agtcaattcc tttgctgcaa tcttaacgtg
     4321 ctcaggttct ttcatgaact tgacaccaat acgcttgatt ggctctggca tggttgcaga
     4381 aaacagaagg gtttgacggc tctctggtac acgttcgatg atcgcttcaa tatcatccaa
     4441 gaagcccatg ttgagcattt catctgcctc gtcaagaatg agagtttcaa caccgtcaag
     4501 tttgagggcc ttgcgtttga tcaagtccaa gaggcgacct ggtgtaccaa caacgatgtg
     4561 agcacctgaa cgaagggcct tgatttgctt ttcaatgctt gagccaccgt aaacagagcg
     4621 gactttcaca cccttttcac gaccaaattt gaaaagttct tcttgactct gcaccgccaa
     4681 ctcacgtgtt ggggcgataa tcaaggcctg aattgcttga ttgtttgtgt caattttgtt
     4741 gagggttggc aaaccaaagg ctgcagtttt ccctgtcccc gtctgggctt gaccaatcac
     4801 atctttacct tcaagagcaa gcggaatggt ctgctcctgg attggtgacg gcgtttcaaa
     4861 accagccttc tcaacggcaa gcaaaatatc ttctgataat tgtaaatctg taaatttcaa
     4921 tgtattctct tttctaaaag gcagtgcgaa gctaccttat ctaacttttt tgtaacaaga
     4981 tatcagggtc gaaccactga tattggattt gcaacttacc aag
//
LOCUS       DBNFEG010000150         4988 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.150, whole genome shotgun sequence.
ACCESSION   DBNFEG010000150 DBNFEG010000000
VERSION     DBNFEG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.150"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..714)
                     /locus_tag="AC1PNG_001756"
     CDS             complement(<1..714)
                     /locus_tag="AC1PNG_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HIP6702239.1"
                     /translation="MSKREKKKKRKGSFWRNFLTVVLILISLALIFNTSIRNFIIGWN
                     TNKYQISNVTTEDIEKNKQAETTFDFEQVQSISTEAILAAQWDAQRLPVIGGIAVPEL
                     GINLPIFKGVFNTSLMYGAGTMKENQEMGKGNYALASHHIFGVTGAADVLFSPLDRAK
                     NGMKIYITDKTNVYTYVIDSVEIVSPESVYVIDDVEGRTEVTLVTCTDYYATQRIVVK
                     GVLESTTPYNETAKDILDSF"
     gene            complement(721..3165)
                     /gene="gyrA"
                     /locus_tag="AC1PNG_001757"
     CDS             complement(721..3165)
                     /gene="gyrA"
                     /locus_tag="AC1PNG_001757"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIP6702240.1"
                     /translation="MQDKNLVTVNLTNEMKSSFIDYAMSVIVSRALPDVRDGLKPVHR
                     RILYGMNELGITPDKPHKKSARITGDVMGKYHPHGDSAIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALEMLRDINKNTVNFADNYDASEREPEVLPAR
                     FPNLLVNGTTGIAVGMATNIPPHNLGETIEAVKLVMDNPEVTTREIMEVLPGPDFPTG
                     ALVMGKSGIHRAYETGKGSIVLRSRTEIEEYGNGRERIVVTEFPYMVNKSKVQEHIVK
                     LVQEKRIEGITAVRDESNREGVRFVIEVRRDASANVILNNLFKQTQLQTNFSFNMLAI
                     QNGVPKILSVRQILESYIEHQKEVVTRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRNSETDAIAQAELMEKFDLSVRQSQAILDMRLRRLTGLERDKIQSEYDELVALIAD
                     LADILAKPERVVQIIKDELEEVKRKYADPRRTELMVGEVLSLEDEDLIEETDVLITLS
                     NQGYIKRLAQDEFQAQKRGGRGVQGTGVKDDDFVRELVSTSTHDRLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLEENEAIQTIINVTKDQEADSYLFFATRQGVVKRTS
                     VSEFANIRQSGLKALNLKEDDELINVFLTNGQADIIMGTKFGYSVRFTETDVRNMGRT
                     ATGVRGINLREGDQLVGATMISDDQEVLVLTEKGFGKRTPASEYPTKGRGGKGIKTLK
                     VADKNGSLAGLTTVSGDEDIMVITDTGVIIRTSVANISQTGRSTMGVKVMRLNDEAKI
                     MTFALVDAAEIKEEKE"
     gene            3354..4337
                     /locus_tag="AC1PNG_001758"
     CDS             3354..4337
                     /locus_tag="AC1PNG_001758"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HIP6702241.1"
                     /translation="MTATKQHKKVILVGDGAVGSAYAYALVNQGIGQELGIIDINKDR
                     TQGDAEDLSHALAFTFPKKIYSAEYSDAHDADLVVLTAGLPQKPGETRLELVEKNLRI
                     NQQIVTEIVNSGFNGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQAL
                     AEKIGIDARSVHAYIMGEHGDSEFAVWSHANVAGVKLYDWLQDNRDIDEQGLVDLFVS
                     VRDAAYSIINKKGATYYGIGVALARITKAIFDDENAVLPLSVYQAGQYEGVEDVFIGQ
                     PAIIGAHGIVRPVNIPLSDAELQKMQASAKQLKDIIDDAFANPEIAAGVKN"
     gene            complement(4380..4715)
                     /locus_tag="AC1PNG_001759"
     CDS             complement(4380..4715)
                     /locus_tag="AC1PNG_001759"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIP6702242.1"
                     /translation="MIEGYQEKQFIVASRTEQTDYGNVRFVRDIIGEIMELRSREGGD
                     IWLFGGASLVQDCLEAGVIDHLIIGIIPTILGDGIPLFASLLEEKKLLLVESTVTDGI
                     AMLRYDIRR"
BASE COUNT         1464 a         1142 c          927 g         1455 t
ORIGIN      
        1 gaaggaatct aagatgtctt ttgccgtttc attatatgga gtggttgatt caagaactcc
       61 ttttacaaca atacgttgcg tagcataata gtccgtacat gtcactaacg taacttctgt
      121 acgtccttct acatcatcaa tgacatagac actttcaggc gaaacaattt ccacactatc
      181 aataacgtag gtgtaaacat tggtcttgtc agtaatatag attttcatgc cgtttttagc
      241 acgatcaagc ggtgaaaaga gaacatctgc cgcaccagtt acaccaaaaa tatggtggct
      301 agccaacgca taatttcctt tccccatctc ttggttttcc ttcatggtac cagctccata
      361 catgagcgaa gtattgaaga cccctttaaa aataggaagg ttgataccaa gctcgggaac
      421 cgcaatccca ccaattacag gtaagcgttg tgcatcccac tgagctgcta aaatagcttc
      481 tgtagaaata gactgaacct gttcgaaatc aaatgttgtc tcagcctgtt tattcttttc
      541 gatatcctct gtcgtaacat tgctaatctg gtatttattc gtattccagc cgataataaa
      601 attacggata gaggtattaa atatcaatgc caaggaaatc agtatcaata caactgttaa
      661 aaaattgcgc caaaaagaac ctttacgctt cttctttttt tcacgttttg acatgattat
      721 ttattccttt tcttctttaa tttcggcagc atctaccaag gcaaaggtca tgattttcgc
      781 ttcatcattc aagcgcatca cttttacacc catggttgaa cgaccagttt gagagatatt
      841 ggcaacactt gtacgaataa tcacaccagt atcggtaata accataatat cttcatcacc
      901 agataccgtt gtcaaacctg ccagagaacc attcttatca gcaactttaa gagtcttgat
      961 acccttacca ccacgacctt tggttggata ctcactggct ggtgtacgtt taccaaaacc
     1021 tttttccgtc aatacgagta cttcttggtc atcagaaatc atagtagcgc caactaactg
     1081 gtctccctcg cgtaaattga taccacgaac acctgttgca gtacggccca tattacgaac
     1141 atctgtttca gtgaaacgta cagaataacc gaacttagtc cccataataa tgtctgcttg
     1201 accatttgtt aagaaaacat tgatgagttc atcgtcttct ttcaaattca aagctttcag
     1261 accactctga cgaatattgg caaattctga cacactagtc cgttttacaa caccttggcg
     1321 ggttgcaaag aagagatagc tatctgcctc ctggtcctta gttacattga taatggtttg
     1381 aattgcctcg ttttcttcca atttaagcaa attgaccact ggaagtccct ttgcagtacg
     1441 accatattct gggatttcat agcctttgag acggtaaaca cgtcccttat tagtgaagaa
     1501 gagcaagcga tcatgagtgc ttgtagagac taattctcgt acaaaatcat catccttgac
     1561 gccagttcct tgaaccccac gtcctccacg cttctgagct tgaaactcat cctgtgccaa
     1621 acgcttgatg tagccttggt ttgacaaggt gattaaaaca tccgtttctt caatcaagtc
     1681 ctcatcttca agggatagaa cttcaccaac catcaattct gtacggcgtg ggtcagcata
     1741 tttacgtttg acttcttcta attcgtcttt aataatctga acaacacgct caggcttagc
     1801 tagaatgtca gccaaatctg caatcaacgc cactaactca tcatattctg attgaatctt
     1861 gtcccgttcc aaacctgtca aacgacgaag acgcatatca agaatagcct gactctgacg
     1921 tacagaaagg tcaaatttct ccatcaattc agcttgagca atagcatcag tttctgaatt
     1981 acggataata cgaatgactt catcaatatg gtcaagagca atcagcaagc cttctaagat
     2041 atgagcacgc gcctcagcct tttctttatc aaatcgtgta cgacgagtga ctacttcttt
     2101 ttggtgttca atataagact ctagaatctg acgtacagaa aggattttcg gtacaccatt
     2161 ttggatggcc aacatgttga aactaaagtt ggtttgaagc tgtgtttgct tgaataggtt
     2221 attcaaaata acgttagcag aagcatcacg acgaacttcg ataacaaatc gtacaccctc
     2281 acggttggat tcgtctcgaa cagcagtgat tccttcaata cgtttttctt ggactaattt
     2341 aacaatatgt tcttgaacct tggacttatt gaccatgtat ggaaactctg taacaacaat
     2401 gcgttcacga ccatttccat attcttcaat ttctgtgcgg gaacgaagaa ctattgaacc
     2461 tttaccagtt tcataagcac ggtgaattcc agatttcccc ataaccaagg cacctgtcgg
     2521 aaaatctggt ccaggcaaga cttccataat ctcacgtgtt gttacctcag gattgtccat
     2581 aaccagctta actgcctcga tagtctcacc caagttgtgt ggaggaatgt tggtagccat
     2641 accaacggcg ataccagtcg ttccgttaac cagaaggtta gggaaacgag caggtaaaac
     2701 ctcaggctca cgttcactcg catcatagtt atctgcaaaa tttaccgtgt tcttgttaat
     2761 atcacgaagc atttcaagcg caatcttgct catgcgtgct tctgtataac gctgagcggc
     2821 tgctccgtcg ccatccatgg aaccaaagtt tccgtgacca tcaactaaca tgtgacggta
     2881 gctccaccat tgtgccatac gaaccattgc ttcgtaaatc gcactatctc catgcgggtg
     2941 atatttaccc ataacatccc ctgtgatacg ggctgatttt ttatgtggtt tatccggtgt
     3001 aattcctaat tcattcatcc catacaaaat acggcgatga accggcttca gaccatcacg
     3061 tacatcaggc agagcacgtg aaacgataac actcatcgca tagtcgatga aggatgattt
     3121 catctcattg gtcagattaa ccgttactaa atttttatct tgcattatat gcctctttca
     3181 acagtcattg tgcttctatt ataccatatt ggcgaataaa tttctttgtt ggaaactagc
     3241 atgtaaacat tttcaaaggc gctttcacaa gtgacaaaaa gcttaaaaca tgctagaatg
     3301 tattatggtc gggatgaagt catccccaaa taaaactaag gagatgttta gcaatgactg
     3361 caactaaaca acacaaaaaa gtaatccttg tcggtgacgg tgccgtaggt tctgcttatg
     3421 cttatgctct tgttaaccaa gggattggtc aagaattggg tatcatcgat atcaacaaag
     3481 accgtactca aggtgatgca gaagacttga gccacgcatt ggccttcaca ttccctaaaa
     3541 aaatctactc tgctgagtat tcagatgctc atgacgcaga cttggttgta ttgacagctg
     3601 gtttgccaca aaaaccaggt gaaactcgcc ttgaattggt agagaaaaac cttcgtatca
     3661 accaacaaat cgttacagaa atcgttaact ctggtttcaa cggtatcttc cttgttgctg
     3721 ctaaccctgt tgacgtattg acttactcaa cttggaaatt ctctggtttc cctaaagagc
     3781 gtgttatcgg ttctggtact tctcttgact cagctcgttt ccgtcaagca ttggctgaga
     3841 aaatcggtat cgacgcacgt tcagttcacg cttacatcat gggtgagcac ggtgactcag
     3901 aatttgcagt ttggtcacat gccaacgttg ccggtgtgaa attgtacgac tggttgcaag
     3961 ataaccgcga tattgatgag caaggtcttg ttgacttgtt tgtatctgtt cgtgatgcag
     4021 cctactcaat catcaataaa aaaggcgcaa cttactacgg tatcggtgtt gcacttgcac
     4081 gtatcactaa agcaatcttt gatgatgaaa atgcagtact tccattgtca gtttaccaag
     4141 ctggccaata cgaaggtgtt gaagatgtct tcatcggtca acctgctatc atcggtgccc
     4201 acggtatcgt tcgtccagtc aatatcccat tgagcgacgc tgaattgcaa aaaatgcaag
     4261 catctgctaa acaattaaaa gacatcatcg atgatgcttt cgcaaaccca gaaattgcag
     4321 ctggtgttaa aaactaatta tataaaatag taaaaaaggt gagcaatagc tcgccttttt
     4381 taccttctaa tatcataacg taacatagca attccatctg tgacagtgga ctctaccaag
     4441 agcaatttct tttcttccaa aagtgaggcg aaaagtggaa taccgtcacc caaaatagta
     4501 ggaataatcc caataataag gtgatcaatc actcccgcct ctaaacagtc ctgaaccaga
     4561 cttgctccac caaacaacca aatatctcca ccttctctag aacgaagttc cataatctcc
     4621 ccgatgatgt cccttacgaa acgcacatta ccatagtccg tctgctcggt acgactcgca
     4681 acgataaatt gcttttcctg atatccttca atcatttcca acggacaatc ttctaatgat
     4741 tttctcccca tcgggatcgg gaaagaactc gactggtcaa aaacatacta agattagtag
     4801 tgaggaaatc tccgacggga gaaagtactc actacttttt ctttatgata aagtagaggt
     4861 gtcttgttaa gtcgagaact cttttgacgc tagacgtcgc atcaaaaact ggcaaggcac
     4921 ctgttttagg aagaatgtta tcaaagtatg tgggacgcca gacgtcgagt attgaaaatg
     4981 acatcact
//
LOCUS       DBNFEG010000151         4931 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.151, whole genome shotgun sequence.
ACCESSION   DBNFEG010000151 DBNFEG010000000
VERSION     DBNFEG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.151"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            297..2948
                     /gene="adhE"
                     /locus_tag="AC1PNG_001760"
                     /gene_synonym="adhC"
     CDS             297..2948
                     /gene="adhE"
                     /locus_tag="AC1PNG_001760"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HIP6702243.1"
                     /translation="MADKKVVSPEEKLVEARKHVDELVQKGLVALDEFRKLGQEEVDY
                     IVAKASVAALDKHGELAMHAFEETKRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DEVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLIALKTRNPIVFAFHPSAQESSAHA
                     ARVVYEAAVAAGAPENCIQWITKPSMEATSELMKHDGIATILATGGNAMVRAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEVYDEFVTE
                     FKSYKTYFVNKKEKALLEEYCFGVKANSKNCAEGKLNADIVGRPAAWIAEQAGFSVPE
                     GTNILAAEVAEIGEKEPLTREKLSPVIAVLKVEGRAEGLEAARQMVEFHGLGHSAAIH
                     TEDAELAKEFGTIVRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSISDNVS
                     AMNLLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDRAMVELGF
                     LDRIIEQLDLRRNKVVYQIFSDVEPDPDITTVYKGTELMRTFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPTVDFHDLVQKFMDIRKRAFKFPELGKKSKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPAHVTADTGMDVLTHATEAYVSTVA
                     NDFTDGLALQAIKLVFENLESSVKNADFVSREKMHNASTMAGMAFANAFLGISHSMAH
                     KIGGRFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYKADEKYQDIARLLGLPC
                     STPEEAVAAYAQAVYDLGERIGIQMNLKAQGIDEKELKEHSRELALLAYEDQCTPANP
                     RLAMVDHMQEIIEDAYYGYKERPGRIK"
     gene            3363..4847
                     /gene="thrC"
                     /locus_tag="AC1PNG_001761"
     CDS             3363..4847
                     /gene="thrC"
                     /locus_tag="AC1PNG_001761"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIP6702244.1"
                     /translation="MTLVYQSTRDAKNTVSASQAILQGLATDGGLFTPISIPAVDLDF
                     SVLKDASYQEVAKLILSAFLDDFTADELDYCINNAYDSKFDTPAIAPVVKLKGQYNLE
                     LFRGSTIAFKDMALSILPYLMTTAAKKHGLENEIVILTATSGDTGKAAMAGFADVPGT
                     QIIVFYPRDGVSKVQELQMTTQTGANTHVVAIDGNFDDAQTNVKRMFNDEALRAKLAA
                     KKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIAAGDKVNFTVPTGNFGNILAAY
                     YAKQIGLPVGKLICASNDNNVLTDFFSTGVYDKNRTFRVTTSPSMDILVSSNLERLIF
                     HLFDNDAAKTAELMEALNTAGQYDIQGADADILSLFAAAFATEEETAAEIKRVYDESD
                     YIEDPHTAVASAVYKQYVEQTGDQTPTVIASTASPYKFPVVAVEAVTGQSGLSDFEAL
                     AKLHEISGVALPPAVDGLETAPVRHNTVVAAADMQAEVERYLGV"
BASE COUNT         1454 a         1039 c         1079 g         1359 t
ORIGIN      
        1 aaaattttca aaaaaatcta tttacatgga agcgttttct tgataaacta gtatcgtaaa
       61 tgttagagaa agaataaatg ataaagattt gtcttacttt atgtattttt tgaccaaaca
      121 aagcagaaac aaatctgaat gttttctgaa aataggaaaa agtaagcgct ttctataacg
      181 aaacagaaaa aatgtgaaaa aattaacaat ttccctttac aaataaaata aaaaatagta
      241 gaataacatt gtgaaaaagt taacaaatag ttagcgagta tatttggagg aacacaatgg
      301 ctgataaaaa agtagtatca ccagaagaaa agttggtaga agcgcgcaag cacgtcgatg
      361 agcttgttca aaaaggtttg gttgctcttg atgagttccg taaacttggt caagaagaag
      421 tagactatat tgtagcgaaa gcttcggttg cagcccttga taaacatggc gaattggcaa
      481 tgcacgcctt tgaagaaact aagcgtggtg tattcgaaga caaggctact aagaacttgt
      541 ttgcttgtga gcacgttgtc aataacatgc gtcacacgaa aactgttggt gttattgaag
      601 aagatgaagt gacaggtttg actttgattg ctgagccggt cggtgttgtt tgtggtatca
      661 caccaacaac aaacccaact tctacagcaa tctttaaatc attgattgcc ttgaagacac
      721 gtaatccgat tgtctttgcc ttccacccat ctgcacaaga atcttctgct catgcagctc
      781 gtgttgtcta tgaagcagct gtggcagctg gtgctcctga aaactgtatc cagtggatta
      841 caaaaccatc tatggaagca acatctgagt tgatgaaaca tgatggtatt gcaacaatcc
      901 ttgcaactgg tggtaacgca atggtgcgcg cagcttactc ttgtggtaaa cctgcccttg
      961 gtgtaggtgc aggtaacgtt ccagcctatg ttgaaaaatc agcaaatatt cgtcaggcag
     1021 cacatgacat cgttatgtct aagtcatttg acaacggtat ggtctgtgcg tcagaacaag
     1081 cagttatcat tgataaagaa gtttacgatg agtttgtaac agagttcaaa tcttacaaaa
     1141 cttacttcgt taataagaaa gaaaaagctc ttcttgaaga atactgcttc ggtgtgaaag
     1201 ctaacagcaa aaactgtgct gaaggtaaat tgaacgcaga catcgttggt cgcccagctg
     1261 catggatcgc tgagcaggcc ggcttctcag taccagaagg tacaaacatc ttggcagctg
     1321 aggttgctga aatcggtgaa aaagaaccat tgactcgtga gaaattgtca cctgttattg
     1381 ccgtcttgaa agttgaaggt cgtgcagaag gtcttgaagc agctcgccaa atggttgaat
     1441 tccacggtct cggtcactct gcagctattc atacggaaga cgcagaattg gcgaaagagt
     1501 ttggtacaat cgtacgtgct atccgtgtta tttggaactc tccatctact ttcggtggta
     1561 tcggtgatgt ttacaacgca ttcttgccat cattgacact tggttgtggt tcttacggac
     1621 gcaactcaat cagtgataac gtaagtgcta tgaacttgct caacatcaag aaagtaggaa
     1681 gacgtagaaa taatatgcaa tggtttaaag ttccttctaa aacatacttc gaacgtgact
     1741 ctatccaata tctccaaaaa tgccgtgacg ttgaacgtgt catgatcgtt acagaccgtg
     1801 ccatggtaga acttggattc ctagatcgta tcatcgaaca attggacctt cgtcgtaata
     1861 aagttgttta tcaaatcttc tcagacgttg agccagatcc agacatcaca acggtttata
     1921 aaggtacaga attgatgcgt accttcaaac cagatacaat catcgcactc ggtggtggtt
     1981 caccaatgga tgctgccaaa gttatgtggc tcttctacga acaaccaaca gttgatttcc
     2041 atgacctagt tcaaaaattc atggatatcc gtaaacgtgc cttcaagttc ccagaacttg
     2101 gtaagaaatc taagtttatc gcgattccaa ctacatcagg tacaggttca gaagtaacac
     2161 cgtttgccgt aatctctgat aaagcaaaca accgtaaata cccaatcgct gactactcat
     2221 tgacaccaac tgtggcaatc gttgacccag cactcgtatt gacagtacct gcacacgtta
     2281 cagcggatac tggtatggac gtattgactc acgctacaga agcatacgtt tcaacagtag
     2341 ccaacgactt tacagatggt ttagcacttc aagctatcaa actagtattt gaaaaccttg
     2401 aaagctcagt gaagaatgct gactttgtat cacgtgagaa aatgcacaac gcatcaacta
     2461 tggcaggtat ggccttcgcc aacgcattcc taggtatttc tcactcaatg gctcataaga
     2521 ttggtggacg tttccatacc gttcacggtc gtacaaacgc tatcttgctt ccgtacgtta
     2581 tccgctacaa cggtactcgt ccagctaaga cagctacatg gcctaagtac aactactata
     2641 aagcagatga aaaataccaa gacatcgctc gtcttctagg tttgccatgt tcaacaccag
     2701 aagaagctgt tgcagcatac gcacaagcag tttatgacct cggtgaacgc atcggtatcc
     2761 aaatgaactt gaaagcacaa ggtatcgacg agaaagaact caaagaacac tctcgcgaat
     2821 tggctcttct tgcctacgaa gatcaatgta ccccagctaa cccacgtcta gcaatggttg
     2881 accacatgca agaaatcatc gaagatgcct actacggtta caaagaacgt ccaggtcgca
     2941 tcaagtaagg attttcgtca cgatacatcg ttaaagctcc ttgcctaact ttagttatgt
     3001 ctacgtcgct ttgccttgtc tcgtttagaa aatcattcct tagtgattta gcttcacctt
     3061 cgtcaatctg cctagaaaga cttaggacta gaaaaaagtc taggtaggag tagcaaatcg
     3121 tatcctactc agttgaacgg agtatgattt ataggtaaat tgcctccaaa tatcgtaaga
     3181 caatcctcta ttgaaaaata ggggattgtt tgtttagaaa taatggtgga gatcctgtaa
     3241 aaagcgaaag tggttggaaa gttagggttt agccgagaaa aagagacttt tctatctatc
     3301 tttcacaatt ttctgtcaat ttgtggtaga atagaaaaaa tagatttttt atgagggata
     3361 ccatgacatt agtatatcaa tcaacacgcg atgctaaaaa tactgtatcg gctagtcagg
     3421 cgatattgca gggcttggcg accgacggtg gtttgtttac accgatttct attccagcag
     3481 ttgacttgga cttttctgtt ctcaaagacg cttcttatca agaagttgcc aagttgattt
     3541 tgtcggcttt cttggacgat tttacggcag atgagttgga ctactgtatc aacaatgcct
     3601 acgatagcaa gtttgacaca ccagctattg ccccagtggt taagctaaaa ggtcagtaca
     3661 atttggaact cttccgaggt tcaaccattg cctttaagga tatggctctg tccatcctcc
     3721 cttacttgat gacgactgcg gcgaaaaagc atggtttgga aaatgagatt gtcattttga
     3781 ccgcgacttc aggcgataca ggtaaggcag ctatggctgg ttttgcagac gttcctggca
     3841 cgcaaatcat tgtcttctac ccacgtgacg gggtatctaa ggtccaagaa ttgcagatga
     3901 ctacgcagac aggggctaat acgcacgtgg tggccattga cggtaacttt gacgacgccc
     3961 agacaaatgt caaacgtatg ttcaacgatg aggctctccg tgccaagttg gcagcgaaga
     4021 aacttcagtt ttcatctgcc aattctatga acatcggccg cttggtgcca cagattgttt
     4081 actatgtcta tgcctacgct cagctggtta agactggcga gattgcggcg ggcgacaagg
     4141 tcaacttcac cgttccaaca ggaaactttg gaaacatctt ggcggcttat tacgccaaac
     4201 aaatcggtct gccagttggc aagctcatct gtgcttccaa cgacaacaat gtcctaaccg
     4261 acttcttctc gactggcgtt tacgacaaga accgtacctt ccgcgtgacc accagtccgt
     4321 ccatggacat cttggtgtct tctaacttgg agcgcttgat tttccatctc tttgacaatg
     4381 acgctgcaaa aacagcggag ctgatggaag ccttgaacac agctggtcag tatgatattc
     4441 aaggggctga cgcggatatc ctctctctct ttgctgccgc ctttgcgacg gaagaagaaa
     4501 cggctgcgga aatcaagcga gtctatgacg agtcagacta cattgaagac ccacatacgg
     4561 ctgttgcctc agctgtctat aaacagtatg tggagcaaac aggggaccaa actccgactg
     4621 tgattgcttc tacagcaagt ccttacaagt tcccagtagt tgcggttgag gctgtgacag
     4681 gccagtcagg tttgtctgac tttgaagccc ttgcaaaatt gcatgagatt tcaggtgtag
     4741 ctttgccacc agctgtggac ggcttggaaa cggcacctgt ccgccacaat actgttgttg
     4801 cggcagccga tatgcaggct gaagtagagc gttatttggg tgtttaattt cgatttattt
     4861 tatgacaaac tgctcatttt gaacagtttg ttttcttttt gctgtttaaa ttaaaattta
     4921 cagataattc a
//
LOCUS       DBNFEG010000152         4893 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.152, whole genome shotgun sequence.
ACCESSION   DBNFEG010000152 DBNFEG010000000
VERSION     DBNFEG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.152"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..630
                     /locus_tag="AC1PNG_001762"
     CDS             <1..630
                     /locus_tag="AC1PNG_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6702245.1"
                     /translation="RQIDIHNDLDIPKSTITGYVKGRSLPTSGNLQKLADYLNVKKSD
                     LDSRFSNNDVYKEFLLMLEKQLEEKKYLVNSVSKEKIFQKVKERERRDKELIERVKGM
                     NRSVKPEEFLSTITESLEEYNPKNDYGLLMDLALKTSSILFWLDIVFQKRTSEFYIVF
                     EDKNFNENYYTHFYEKINVLFEEIKAAALEEARKDKNDFEDYKLTWGLD"
     gene            994..1824
                     /locus_tag="AC1PNG_001763"
     CDS             994..1824
                     /locus_tag="AC1PNG_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HIP6702246.1"
                     /translation="MVKINANGKEITLLSTNTDYVSLTDIAKYRNPDAPADIIKNWMR
                     SRNTIEFLGAWEKINNENFKLVEFDQFRSEAGLNGFVLSPQKWIKETNAIGITSQSGR
                     YGGTFAHSDIAFEFASWLSPEFKLYIIQDYQRLKQEEAYKNQLDWQVNRYISKLNYTI
                     QTDAIKENIVPTLQPYQISFAYSSEADLINVALFGMTAKEFKNAYPDREGNQRDNATI
                     EQLLVLNNLQSLNAEMIKQGLSQSNRLTELNRIAKEQLDVLYKNNQKALDSLKRLSDK
                     "
     gene            1989..3161
                     /locus_tag="AC1PNG_001764"
     CDS             1989..3161
                     /locus_tag="AC1PNG_001764"
                     /EC_number="2.7.7.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIP6702247.1"
                     /translation="MIMKITEVKKKNGATVYRASVYLGIDAITGKKVKTNVTGRTKKE
                     VKNKTQQAIATFKTDGATRYQNATITSYKELAELWWNSYKHTVKPNTRGNIRGLLKNH
                     VIPLFGAYKLDKLTTPLLQSIVIKLADKANTGEAGAYLHYDKIHALNKRILQYGVVMQ
                     VLPYNPAREVILPRNAKKATRKKVKHFNDEQLKQFLGYLDGLDLTNYRNLYEATLYKF
                     LLATGCRINEVLALHWSDIDLNNATVSITKTLNRYGSINSPKSNASIRDINIDSQTVA
                     MMKEYRRRQIQEAWTLGRSETVVFSDFIHDYPEDKTLGNRLTTRLRNIGLPNIGFHGF
                     RHTHASLLLNSGIPYKELQHRLGHSRISITMDIYSHLSKENAKNAVAFYEKALGNL"
     gene            3299..3682
                     /gene="yaaA"
                     /locus_tag="AC1PNG_001765"
     CDS             3299..3682
                     /gene="yaaA"
                     /locus_tag="AC1PNG_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HIP6702248.1"
                     /translation="MEFKLFDDYITLQALLKTTGILHSGGAIKGFLEENTILFNGEDE
                     KRRGKKIRVGDVITLPDHNTSITIVAPSAEEIKQHQEDQAEKERVAAIVKKLNQENKK
                     NKKQVKTPKKATKNTERKPVRFPGM"
     gene            3685..4779
                     /gene="recF"
                     /locus_tag="AC1PNG_001766"
     CDS             3685..4779
                     /gene="recF"
                     /locus_tag="AC1PNG_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638862.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIP6702249.1"
                     /translation="MWLERLELQHFRNYNQLDIEFHKGLNVFLGENAQGKTNILESIY
                     VLALTRSHRTRTDKDLLQFQEKELSISGLLHRTSGKVPLDIHLTDKGRVTKVNHLKQA
                     KLSNYIGHMNVVLFAPEDLQLIKGAPALRRKFIDVELGQIKPLYLSDLSNYNHVLKQR
                     NTYLKSTDKIDENFLSVLDQQLAEYGSRVIQHRIDFLKKLEEFGNRKVQEISGNREEL
                     TIEYQTSIKLTDDVNLIDKFLTELERCRKRDLFKKNTGVGPHRDDVAFFINGMNAHYG
                     SQGQHRSLVLSLKLAEIELMKEVTREYPILLLDDVMSELDNNRQIKLLETITDTIQTF
                     ITTTSLDHLHKLPDSLKIFHIESGKVTESE"
BASE COUNT         1745 a          971 c          910 g         1267 t
ORIGIN      
        1 cggcaaatag atattcataa tgatctagat ataccaaaaa gtacaatcac agggtacgtt
       61 aaaggacgtt cgttgccaac gtcaggaaat ttacaaaaac ttgctgatta tctcaatgta
      121 aaaaagtcgg accttgattc tcgcttctca aacaatgatg tctataaaga atttctgtta
      181 atgcttgaaa agcaattgga agaaaaaaaa taccttgtaa atagcgtgtc aaaagaaaaa
      241 atttttcaaa aggtaaaaga aagagaacgt agagacaaag aattaattga acgcgttaaa
      301 ggtatgaatc gctctgtgaa acctgaagag tttcttagca ctataactga atcgttggaa
      361 gaatataacc ccaaaaatga ctatggttta cttatggacc ttgctttgaa aacgagtagc
      421 atactttttt ggcttgatat agtatttcaa aaaagaacga gtgagtttta tatagttttt
      481 gaagataaaa acttcaacga aaattactat actcattttt atgaaaaaat taatgtttta
      541 tttgaagaga taaaggctgc tgctttagaa gaagctagaa aagataaaaa tgattttgaa
      601 gattataagt taacgtgggg attagactaa ccgcccacgc tccctacacg ccccctcacc
      661 acgttcaaac acttctcggg tatatttacc ctgccaagct attccacgcg cccaggggct
      721 ttctagtgga ctgtgtggag tgaaaaaaag tccgcattaa aaaaagtcag tttttatcag
      781 tagatgaaaa agtaaggttt tgtaaggttg tatattttag caacctcaat aaacctcaac
      841 ttctatatta gttgtatatt agcatttgtt agcaatctgc gcgtgctttc ctcgtgcttc
      901 agcaagtccg ctgacttagt aaaggttata cctatcaatt ttgttgacgc caacaaaact
      961 gtaaatccta taacaatttt agaaaggctc ataatggtta aaattaacgc aaatggtaaa
     1021 gaaataactt tactttccac aaatactgat tatgtcagtc tgacagatat cgctaaatat
     1081 cgaaatccag acgctccagc cgatattatt aaaaactgga tgaggagtag aaatactatt
     1141 gaattcctag gagcttggga aaaaataaat aatgaaaatt ttaaactggt cgaattcgac
     1201 cagtttagaa gcgaggctgg attaaatggc ttcgtactat ctccgcaaaa atggataaaa
     1261 gagacaaatg ccattggtat aacttcccaa tctggccgat acggcggtac attcgcccac
     1321 tctgatatag cttttgaatt tgcttcctgg ctttcaccag agtttaagct ttatatcatt
     1381 caagattatc agcgcctaaa acaagaagaa gcctacaaaa accagttaga ttggcaggta
     1441 aaccgctata tttcaaaact taattacacc atacaaacgg acgctatcaa agaaaacatt
     1501 gtgccaactc tccagcccta tcaaatttct ttcgcctatt cctctgaagc tgatttaata
     1561 aacgtcgctt tatttggtat gactgcaaag gaattcaaga acgcctatcc tgatagagaa
     1621 ggtaatcagc gagataatgc cactattgaa cagctattag ttctaaataa cttacaaagt
     1681 cttaacgctg aaatgattaa acaaggacta tcacaaagca accgactaac tgaactaaac
     1741 agaattgcta aagaacagct agacgtgcta tataaaaaca atcagaaggc ccttgatagc
     1801 ctcaaaagat tgagcgataa ataagcccat ataagagtca tagcaccact ttaaaacgta
     1861 accatataaa ttatcatctt acccgaaaca aacgaaaata gggctattct cgtaagccta
     1921 cgcatgatat aaacctaaaa cattctaaaa tctttttaat aacagcttgc ctgctgatgg
     1981 aaaggtttat gatcatgaaa ataactgaag taaaaaagaa aaacggtgct actgtgtacc
     2041 gtgccagtgt ttatctagga atagatgcca tcactggtaa gaaagtcaag actaatgtca
     2101 cgggtaggac caagaaggag gttaaaaaca aaactcagca agctattgca acttttaaaa
     2161 cagacggggc aacacgctac caaaatgcca ccataaccag ttacaaagag ttggcagaat
     2221 tgtggtggaa tagctataag cacacagtaa aaccaaatac ccgtggaaac atcaggggct
     2281 tactgaaaaa tcatgttata ccactttttg gagcctataa gctcgataaa ctgacgaccc
     2341 cactcctcca aagcatcgtc atcaaattag cagacaaagc aaatacaggg gaagctggtg
     2401 cctatctgca ctatgacaaa atccacgcgc tgaacaaacg tatattgcaa tacggtgttg
     2461 ttatgcaagt tctgccatac aatccagctc gggaagtcat attgcccagg aacgcaaaaa
     2521 aagccactcg gaagaaggta aagcatttca acgacgagca gcttaaacag tttcttggct
     2581 acttggacgg cttagactta accaattaca gaaatctgta tgaagccacc ctatacaagt
     2641 tcttgctggc cactgggtgc cgtatcaatg aggtgctggc actgcactgg tcagatattg
     2701 acttgaacaa cgccacggtc agcatcacga agacactaaa ccgctatggg tcaatcaatt
     2761 cccccaagtc aaatgccagc atacgcgata taaacatcga tagccagaca gtagccatga
     2821 tgaaagaata cagacggcga cagatacaag aggcttggac cctcggacgc tctgaaacgg
     2881 ttgtattttc cgactttatc catgactatc ctgaggataa gaccctgggg aacaggttga
     2941 ccacacgcct gaggaatatc ggactgccta acatcggctt tcacggtttc cgccacaccc
     3001 acgctagtct actgcttaac tctggaatac cctacaagga actccagcac cgccttggtc
     3061 attctagaat atcaataacc atggacatct atagccacct ctcaaaagag aatgcaaaaa
     3121 acgctgttgc attttacgaa aaagctctcg ggaatctttg agggtgaaca aaaaggtgaa
     3181 caaattcagt ttacagtact ttacaagact gtaaaacccc ttgatacaac tggggtttca
     3241 gcccctaaaa tcgaaattat accattttgt gatataatat tctgtaacga ggtgacttat
     3301 ggaatttaaa ttatttgatg actacatcac attacaagca ttgttaaaaa cgactggtat
     3361 tctccattca ggaggtgcca ttaaaggttt cttagaagaa aataccattc ttttcaatgg
     3421 agaggatgaa aaaagacgcg gtaaaaaaat ccgtgtcgga gatgttatca cactcccaga
     3481 ccacaacacc agcatcacaa tcgtcgctcc tagtgcagag gaaatcaaac agcaccaaga
     3541 ggatcaagct gaaaaagaac gtgtggctgc aatcgttaaa aagttaaatc aagaaaataa
     3601 aaagaataag aaacaggtaa aaacgcctaa aaaagcaact aaaaatactg aaagaaaacc
     3661 tgttcgtttc cctggtatgt agacatgtgg ctagaacgat tagagttaca acattttcgc
     3721 aattacaacc aattagatat tgagttccac aaagggctca atgtcttttt aggcgaaaac
     3781 gcccaaggaa aaaccaatat tctagaatct atctatgttt tagccttaac acgaagtcat
     3841 cgaacacgga cagataagga cctactacag tttcaagaaa aggaattatc tatttctggt
     3901 ttactccatc gcacaagcgg taaagttccc cttgacattc acttgacaga caaggggcga
     3961 gtgaccaagg tcaaccatct caaacaagcc aagctttcca actatatcgg ccacatgaat
     4021 gttgttctct tcgcccctga agatttacag ctgataaaag gtgcgcctgc tttgagaaga
     4081 aagttcatcg atgtcgagct cgggcaaatc aagccactct atctctctga cctatcgaat
     4141 tataaccacg ttttaaaaca aagaaatact tacctcaaat ctacagataa gattgatgag
     4201 aacttcctat ccgtgttaga tcagcaactt gctgaatatg gtagtcgtgt tatccaacat
     4261 cgtattgact ttctaaagaa attagaggaa ttcggaaata gaaaagttca agaaatatct
     4321 ggcaatcggg aagaattgac aatcgaatac caaacctcaa tcaaattgac agatgatgtc
     4381 aatttaattg acaaattctt gacggaattg gaaaggtgtc gcaaacgtga tttatttaag
     4441 aaaaatacag gagttggacc acatcgagat gatgtagctt tctttataaa tggaatgaat
     4501 gcccattatg gtagccaagg tcagcaccgc agtctcgttc tgtcactcaa actggctgaa
     4561 attgagctga tgaaggaggt cacacgagaa tacccaattc tactcttaga tgatgttatg
     4621 agtgagctag ataataatcg tcaaatcaaa ctactagaaa ctattacaga tacgattcaa
     4681 acatttatta caacgacatc tttagaccat ttacataagc tacctgatag tttaaaaatc
     4741 ttccatattg agtctggtaa ggttactgag tcagaataag attgtttaca ctgttgtcaa
     4801 taaagtttac aaaaaagtaa aaccctgcca agagggtctt gcagcacgta gacaaactcc
     4861 taaaaatata aaaaattgaa gaagtttgcg aaa
//
LOCUS       DBNFEG010000153         4860 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.153, whole genome shotgun sequence.
ACCESSION   DBNFEG010000153 DBNFEG010000000
VERSION     DBNFEG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.153"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            58..945
                     /locus_tag="AC1PNG_001767"
     CDS             58..945
                     /locus_tag="AC1PNG_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIP6702250.1"
                     /translation="MNNQIILLTIQVFIVALILWMLYTILSYAKKHDISLKEKFWTGF
                     GIGYVTDLLDTLGIGTFATTTTMFKLTKLVEDDRKIPATMTTAYIIPVLVEALLFITI
                     VEVDMVTLIAMAAAAFTGAFVGAKVTQKWDTKKVQRILGILLIIAACFMVYRMITNPG
                     ADLTNEVKGLTGWKLAVGIAFDFMVGMLMSMGLGNYAPELIFFSLMGISPAVALPVMM
                     LNAAMIMTAGAKQFIQSGRVNWPGVPGIVVGGVLGVLTAAFFLSNLDINNLKILVVFI
                     AAYTGFTLLRSSFVTRIKK"
     gene            957..1343
                     /locus_tag="AC1PNG_001768"
     CDS             957..1343
                     /locus_tag="AC1PNG_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HIP6702251.1"
                     /translation="MDIHLRNEIDLDGQIEVVDQQFQVEVKEKDGNLYLIYSNDEAEK
                     VVIKCDEEELVMTRFSTPKSIMRFISGKEAIVTIPTPLGIQHFVTDTKRYQLNRSDQS
                     VQLQYELKGLENQQLFASYDLEISWK"
     gene            1553..4240
                     /locus_tag="AC1PNG_001769"
     CDS             1553..4240
                     /locus_tag="AC1PNG_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIP6702252.1"
                     /translation="MSKEQKRDLFYTQGEEQVLSSMESSLDGLSSDEASKRLADYGRN
                     ELDEGEKRTLLAKFLDQFKDLMIIILIAAAILTVITEGSHGLTDAIIILTVVILNAAF
                     GVYQEGQAEAAIEALKNMSSPVARALRDNHVVEVDSKDLVPGDIVLLEAGDVVPADMR
                     LLEANSLKIEEAALTGESVPVDKDLSAVLAEDAPIGDRVNMAYQNSNVTYGRGLGVVT
                     NTGMYTEVGHIAGMLANADETDTPLKQNLHQLSKVLTYAVLVIAAVTMAVSVFVRGEG
                     ILPALMTSVALAVAAIPEGLPAIVTVVLSLGTQVLAKRNSIIRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGQLVDAKEALDASNTTLRVMNFANDTKVDPTGKLIGDP
                     TETALVQFGLDQNFDVREVLVSEPRVAELPFDSTRKLMSTVHQQAAGNFFVAVKGAPD
                     QLLKRVTQIEENGTIRPITDADKQAILATNKDLAKQALRVLMMAYKYVDAIPELESDV
                     VENDLVFSGLVGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGIIDP
                     NDTEDHVFTGAELNELTDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNDGKVVAMTGD
                     GVNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKT
                     IQYLLSANTAEVLCIFLATLFGWDVLEPVHLLWINLVTDTLPAIALGVEPAEPGVMQH
                     KPRGRNSSFFSGGVLSSIIYQGILQTALVLGVYGFALLYPEHAGDNHAIHADALTMAY
                     VTLGLIQLVHAFNVKSVYQSIFTVGPFKNKLFNWSIVAAFLLLMATVVIPGFNTFFKV
                     SILTPTQWMVTVIGSGLMVVVVEIVKFVQRKLGLDEKAI"
     gene            complement(4356..4745)
                     /locus_tag="AC1PNG_001770"
     CDS             complement(4356..4745)
                     /locus_tag="AC1PNG_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702253.1"
                     /translation="MRVIIHYPKPIKQLSSQKLPLLVDGKLAGTVTQGKILSLPITQP
                     SVTLSIRGDKKSAIQVKEGDRVQIMENSRYFILYYFSLAIVPIALLFNLPTLVKTILL
                     ILALAITLLGQAIFPKYIISTEKSSDH"
BASE COUNT         1352 a          904 c         1098 g         1506 t
ORIGIN      
        1 tctaaaacac aaggatattg ctatgtaccc aatggaatct tttggtataa tggagaaatg
       61 aataatcaaa ttatcttatt aactattcag gtctttattg tagccctgat tctttggatg
      121 ctttacacaa ttctatccta tgctaaaaag catgatattt ctctaaaaga gaaattttgg
      181 acaggatttg gtatcggtta tgttactgat ttgctagaca ccttggggat tggaaccttt
      241 gcaacaacaa ccactatgtt taaactgaca aagttggtgg aagacgatcg taaaattcct
      301 gcgaccatga caacggcgta catcatacca gtcttagtag aggcgctatt gtttattacg
      361 atagttgagg ttgatatggt cactctgatt gctatggcag cagcggcatt tacgggtgcc
      421 tttgttggtg ccaaggtaac tcaaaaatgg gatacaaaga aagttcaacg aattcttggg
      481 attttactaa ttattgcagc ctgtttcatg gtctatcgca tgattactaa ccctggcgca
      541 gatcttacca atgaagtaaa gggcttaact ggttggaaac tggctgttgg tattgccttt
      601 gattttatgg tgggaatgct gatgagtatg ggactgggta actatgctcc tgagttaatt
      661 ttcttctcat tgatgggaat tagtccagcg gttgctcttc cagttatgat gttgaatgct
      721 gctatgatta tgacagctgg tgccaagcag tttatccaat caggacgagt aaactggcca
      781 ggagttccag gaatcgttgt cggtggggtg ctaggtgttt tgacagccgc tttcttccta
      841 tcaaatttgg atattaataa tttgaaaatt ttagttgtct tcattgcagc gtatacaggc
      901 tttactctgc ttcgttcatc ctttgttact aggattaaaa aatagtagga ggaactatgg
      961 atattcattt acgaaatgaa attgatctgg acgggcagat tgaggtagtg gatcagcaat
     1021 ttcaagtgga agttaaggaa aaagatggga atctctatct catttatagt aacgatgaag
     1081 ctgaaaaagt tgtcataaaa tgtgacgaag aagagttagt gatgacacgt ttttccactc
     1141 cgaagtctat tatgcgattc atttctggta aagaagctat tgtgaccatt cctacaccgt
     1201 taggaatcca gcattttgtg accgatacaa aacgttacca attaaaccgt tcggatcaaa
     1261 gtgtccagtt gcaatatgaa ttaaagggat tggagaacca gcagcttttt gcttcctatg
     1321 atttggaaat ctcgtggaaa taatctctta tttccagaaa aatattcttg aatttctcgg
     1381 gatttttgat ataatagtta acaaatcgag ggagtagcta gcggaaaatt ccgtgatatt
     1441 gtcgtcaatc cgacttgttt ccggcaatat attaaatagc gagacttgtt ttaataaaac
     1501 aggtctcttt ttattgtaga aggtagagaa gaggccgaaa aggagaagga aattgtcaaa
     1561 agaacaaaaa cgcgatttgt tttatacaca aggcgaagag caagtattgt caagcatgga
     1621 gtcttcttta gacggtttgt ctagtgatga ggcaagcaaa cgtctagcag actacggtcg
     1681 taacgaattg gatgagggtg aaaaacgcac actcttagct aaattcttgg atcagttcaa
     1741 agacttgatg attatcattt tgattgcagc agcaatcttg acggtgatta cagaaggttc
     1801 acacggtttg acagatgcca ttatcatctt gaccgtggtt atcttgaacg ctgcctttgg
     1861 tgtttatcaa gagggtcaag ctgaggcagc tattgaggcg ctaaaaaaca tgtcaagccc
     1921 agttgcgcgt gcgcttcgtg acaatcatgt agtcgaagtt gattctaaag acttggtacc
     1981 aggtgacatc gtcttgcttg aagcaggtga tgttgtccct gccgatatgc gtttgttgga
     2041 agctaactcc ctcaaaatcg aagaagcagc tcttacaggt gagtctgtac cagttgataa
     2101 agatttgtca gcagttttgg ctgaagatgc accaatcggt gaccgtgtca acatggctta
     2161 ccaaaactct aatgttactt acggccgtgg tcttggtgtg gttacaaata caggtatgta
     2221 cacagaggtt gggcacatcg ctggcatgct tgctaatgca gatgagactg atacaccatt
     2281 gaagcaaaac ttgcaccagc tatcaaaagt cttgacatac gcagttcttg taattgcagc
     2341 ggtaacgatg gctgtttcag tattcgtacg tggagaaggt atcttgcctg ctcttatgac
     2401 ttctgtagct cttgcggttg ctgccattcc agaaggtttg ccagctattg ttacagttgt
     2461 tctttcactt ggtacacagg tcttggctaa gcgtaattcc atcatccgta agttgcctgc
     2521 tgttgaaaca cttggttcaa ctgaaatcat cgcttcagat aaaactggta ctttgactat
     2581 gaaccagatg actgttgaaa aagtttatac aaacggtcaa ttggtggatg ctaaagaagc
     2641 tttggatgca agcaatacaa cgcttcgcgt gatgaacttt gccaacgaca caaaagttga
     2701 cccaactggt aaattgatcg gtgacccaac tgaaacggca cttgttcaat ttggtttgga
     2761 tcaaaacttt gacgttcgtg aagtcttggt atctgagcct cgtgtggctg aattgccatt
     2821 tgactcaaca cgtaaattga tgtcaactgt tcaccaacaa gctgctggaa acttctttgt
     2881 agctgttaaa ggtgccccag accaattgct caaacgtgta acgcaaattg aagaaaacgg
     2941 tacaatccgt ccgattaccg atgcggataa acaagccatt cttgccacta acaaggactt
     3001 ggctaaacaa gcccttcgtg tcttgatgat ggcatacaag tatgttgatg ctattcctga
     3061 attggaatct gacgttgttg aaaacgacct tgtcttctca ggtttggttg gtatgattga
     3121 ccctgagcgt cctgaagcgg cggaagctgt tcgtgttgct aaggaagctg gtatccgtcc
     3181 aatcatgatt acgggtgacc accaagatac agctgaggcg attgcaaaac gtcttggtat
     3241 catcgatcca aatgatacag aagaccatgt atttacaggt gcagagctca acgaattgac
     3301 ggatgaagag ttccaaaaag tcttcaaaca atattctgta tacgctcgtg tatctccaga
     3361 acataaggtt cgtatcgtta aggcttggca aaatgatggt aaggttgttg ccatgactgg
     3421 tgacggtgtt aacgatgcgc catcacttaa gactgccgac attggtatcg gtatgggtat
     3481 cactggtacg gaagtttcga aaggtgcctc tgatatggtc cttgcagatg acaactttgc
     3541 aactatcatt gtggcggttg aagaaggacg taaagtcttc tcaaacatcc aaaaaaccat
     3601 ccagtatctc ttgtcagcta acactgctga ggtattgtgt atcttccttg ctaccctctt
     3661 tggttgggac gtattggagc cagttcatct tctttggatc aatttggtaa cagatacact
     3721 tccagcgatt gcccttggtg ttgaacctgc tgaaccaggt gttatgcagc acaaacctcg
     3781 tggtcgtaac tcaagcttct tctcaggtgg tgtcttgagt tcgattatct atcaaggtat
     3841 ccttcaaaca gcacttgtct tgggcgtata tggttttgca cttctttacc cagagcatgc
     3901 tggagataac catgccattc atgcggatgc tcttacgatg gcatatgtta ctcttggatt
     3961 gattcagttg gtgcatgcct tcaacgttaa gtctgtttac caatctatct ttaccgttgg
     4021 tccattcaag aacaaactct ttaactggtc tatcgtagct gcattcttgc tcttgatggc
     4081 gactgttgtg attccaggat tcaatacctt cttcaaggta tctatcttga caccaactca
     4141 atggatggta acagtaatcg gttcaggtct catggttgtc gttgtagaaa ttgttaaatt
     4201 tgttcaacgt aagttgggat tggatgaaaa agctatctaa tatagttatt cagcttatct
     4261 tttattactt cgttaactcg acttgccgta cttcagtaca gcctgcgcct cgttaccttg
     4321 tactaaaagc aaatatcatt tgaaaaagct cagatttagt ggtctgagct tttttctgta
     4381 ctgataatgt attttgggaa gattgcttgt cccaacaacg taatggctaa tgctagtatg
     4441 aggaggatgg ttttgacaag agttggtaag ttaaacagta aggcgattgg tacgatagcc
     4501 aagctgaagt agtagagaat gaagtacctg ctattttcca ttatttggac cctatcgcct
     4561 tccttaactt gaatggctga ttttttatcc cctcgaatgc ttagtgtaac agatggttgt
     4621 gtaattggta agcttagaat tttaccttga gtaactgtcc cagcaagttt tccatctacc
     4681 aggagcggaa gtttttggga ggatagttgt ttgataggtt ttggataatg aataataact
     4741 ctcatatgaa cctccttgta atctagtttc attatagcaa aaaaagaaaa aatgtaaacg
     4801 gtttctgaca ctgttttccc ctacaggctt gcttgaagaa taagaaactg ctataatttt
//
LOCUS       DBNFEG010000154         4859 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.154, whole genome shotgun sequence.
ACCESSION   DBNFEG010000154 DBNFEG010000000
VERSION     DBNFEG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.154"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            42..1349
                     /locus_tag="AC1PNG_001771"
     CDS             42..1349
                     /locus_tag="AC1PNG_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702254.1"
                     /translation="MKKISDLGLTGRKLVGEGLILVFIGIGFLIAGWQFPGLILRFVH
                     AGLFFLALYELSMTFFRKKKSSESVIALVGKAVLFGILASLDLAVQIPLYFAAIFVGI
                     YQLFTALINFITFYLYRKDGVQPRIRYLIDGIWLSLLGSASLFVSGTQLVVQTIVIGG
                     YLILYGLTNLRDGFLFEEAIEQQSLKRHVRLTLPLFLAALVPRMTLQKVNDYLADNEG
                     QTAQSIYNRHKEIAEFPALEVFVHVGEEGFGAVGHVDLSYKGQVYGFGSYDVLSERLG
                     GAIGDGVLFRAERQAYIDFCNQEGMTMLGYQLALSSEQEKAVETRLAEIEGLLLPWQP
                     SAEKVSRRSDGQPIEMYAYRMKEAIGAVLFKFKKSKFKTYFVLSTNCVLLADSVIGQA
                     GTDVLGLRGFIAPGTYQSYLDQEYEKPHSLVVAKNIYYRKEKS"
     gene            1425..4061
                     /gene="polA"
                     /locus_tag="AC1PNG_001772"
     CDS             1425..4061
                     /gene="polA"
                     /locus_tag="AC1PNG_001772"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIP6702255.1"
                     /translation="MKKNKLLLIDGSSVAFRAFFALYNQIDRFKSPTGLHTNAIYGFN
                     LMLDHMMKRIEPTHILVAFDAGKTTFRTEMYADYKAGRAKTPDEFREQFPFICQMLDA
                     MGVKHYELAQYEADDIIGTLDKMAERTEIPFDVTIVSGDKDLIQLTDENTVVEISKKG
                     VAEFEEFTPAYLMEKMGITPTQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLTEFGSL
                     DGIYENIDSMKASKMKENLIADKEKAFLSRTLATIDTQAPIEIGLDDIVYQGPKVDEL
                     GQFYDDMGFKQLRAQLGTTSSQEEAVLDFQIVTEISPAMLKQDQFFYFEILGENYHRE
                     DLVGLAWGDKEKIYVGGPELLDSPVLRDFLENQTIKTYDFKRGKVLLDRKEITLPPAT
                     FDSRLAKYLLSTVEDNSLTTIANLYGQISLVPDEAVYGKGAKLDLPEREIFFPHLARK
                     VQVLIETEEPMLTKLEENQQLDLLFDMELPLANVLAKMEIAGIKVEAETLKAMQSENE
                     VLIDQLTKEIYELAGQEFNINSPKQLGTILFEEMGLPLEYTKKTKTGYSTAVDVLERL
                     APIAPVVSKILEYRQITKLQSTYVVGLQDAILEDGKIHTRYVQDLTQTGRLSSTDPNL
                     QNIPVRLEQGRLIRKAFVPSLENSVLLASDYSQIELRVLAHISQDKHLIEAFQQGADI
                     HTSTAMRVFGIEKAEDVTPNDRRNAKAVNFGVVYGISDFGLSNNLGITRKEAKAYIDT
                     YFERFPGIKNYMETIVREARDKGYVETIYKRRRELPDINSRNFNVRNFAERTAINSPI
                     QGSAADILKVAMINLDKALTEAGLATRMLLQVHDEIVLEVPVAELETVKAMVKETMES
                     AISLSVPLIADENEGSTWYEAK"
     gene            4048..4830
                     /locus_tag="AC1PNG_001773"
     CDS             4048..4830
                     /locus_tag="AC1PNG_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIP6702256.1"
                     /translation="MKQSKKGKSMKKLILCIGLCSLLLAGCGKASQKTEKTPASSGEE
                     FSTTLPILQEKQDTTNEQFNVLANAEVVAVNSTPPADETQKGHKIYAANNGVAEKDEN
                     GNIKEYFRYYNVPSTWTINAENTKDETVAAVYDVKEGGSNYMVQLYNINAFNQSPLED
                     GRNMTPEELEARMVETNHSFNEKTVVTINGQEWRVGRQLLTDQKLARLTFYRMESTAA
                     YDDSVVVGSIYYSLDPGTDKDRTNLKKVIGQVKDVVYQIAKK"
BASE COUNT         1451 a          947 c         1103 g         1358 t
ORIGIN      
        1 tttctgtaaa cacaagtcta gaagtgtgct ataatcatct catgaaaaaa ataagtgatt
       61 taggattaac aggtcgaaaa ttagttggag aaggtctaat cttagtcttc atcgggattg
      121 gatttttaat tgctggttgg caatttccag gactgattct tcgatttgtt cacgcaggtc
      181 tattcttttt agctttatat gaattgagca tgacattttt cagaaagaag aaatcgagtg
      241 aaagtgtcat tgcgctagtt gggaaggctg ttcttttcgg gatcctagcc agtctcgatt
      301 tagctgttca aattcccctc tatttcgcgg ctatttttgt tgggatttat caacttttta
      361 cagctcttat caatttcatt actttttacc tatatcgaaa agatggagta cagccacgga
      421 ttcgctattt aatagatgga atttggttat ccttactagg ttcagcttcc ctatttgtgt
      481 caggcacaca gctggttgtt caaaccattg tcatcggagg ttatctgatc ttgtatggac
      541 taactaatct gcgtgatggc ttcttgtttg aggaggcgat tgagcaacag agtctaaagc
      601 gccatgtacg cctaaccctc cccttgttcc ttgcagccct tgtaccacgg atgaccctgc
      661 aaaaagtcaa tgattacttg gcggataatg agggacaaac ggctcaatct atctataacc
      721 gccacaaaga aattgcggag tttccagcat tagaagtctt tgtacacgtt ggagaagaag
      781 ggttcggagc agttggacat gttgatttga gttacaaagg gcaggtctac ggttttggtt
      841 catatgatgt cctgtcagag cgtttgggtg gtgcgattgg tgacggagtt ttgtttagag
      901 ctgagcgtca ggcctatatt gatttttgta accaagaagg aatgactatg ttgggctatc
      961 aattggcatt gagttctgag caggaaaaag cggtagaaac tcgactagct gaaatagaag
     1021 gtttactgct tccatggcaa ccaagtgcgg aaaaggtctc tagacggtca gacggccagc
     1081 ctattgaaat gtatgcctac cgcatgaaag aggcaattgg ggcagtactt ttcaaattta
     1141 aaaaatcaaa attcaaaacc tactttgtcc tctcaaccaa ctgcgtcctt ttggctgatt
     1201 cagtaatcgg tcaagcagga acagatgtcc taggcttgcg cggtttcatt gctccaggaa
     1261 cctaccaatc ttatctggat caagaatacg aaaaacctca tagtttagta gtcgcaaaga
     1321 acatttatta tcgaaaagag aaatcctagt cactgactgg gattttctgt tattccagct
     1381 ctctaagttc cacaaaactc ccccatccat gctataatag agacatgaaa aaaaataaat
     1441 tattattaat tgatggctct tcggtagcct tccgtgcttt ctttgcactt tataatcaaa
     1501 ttgatcgttt taagagtccg acaggtcttc acaccaatgc tatctacggc tttaacctca
     1561 tgctggacca catgatgaag cggattgaac cgacccacat tctcgtggct tttgatgctg
     1621 gaaagaccac cttccgtacg gagatgtacg ccgattataa ggcaggtcgt gccaagacac
     1681 cagacgagtt ccgtgaacaa ttccccttca tctgtcagat gctggatgct atgggggtca
     1741 agcattatga gctggctcag tacgaggcag acgatattat cggtaccttg gacaaaatgg
     1801 cagagcggac agagattcct ttcgatgtga ccattgttag cggtgataag gacttgatcc
     1861 agctgacaga cgaaaacacc gtagttgaga tttctaagaa aggcgtagcc gaattcgaag
     1921 aatttactcc agcctacctc atggaaaaaa tggggattac ccctactcag tttattgacc
     1981 tcaaggcttt gatgggagat aagtccgata acattcctgg cgtgaccaaa atcggtgaaa
     2041 aaacaggtct aaaactcctg acagaatttg gttctttgga tggtatctac gaaaacattg
     2101 acagcatgaa ggcttctaag atgaaagaaa acctgattgc tgataaggag aaggccttcc
     2161 tatctcgtac acttgctacc attgatactc aggcaccaat cgaaattggt ctggatgaca
     2221 ttgtttatca aggccccaag gtggatgaac taggacaatt ttatgatgac atgggcttca
     2281 aacaattaag ggctcagtta ggtacgacca gctcgcaaga ggaagcggta ctcgatttcc
     2341 aaatcgtaac ggaaatcagc cccgctatgc tcaaacaaga ccagtttttc tattttgaaa
     2401 tcttgggtga aaattatcac cgagaggatt tggttggttt agcttgggga gataaggaga
     2461 aaatctatgt cggagggcct gagctacttg atagtccagt cttgcgtgac tttttagaaa
     2521 accaaactat aaaaacctat gactttaagc gtggaaaagt tcttctcgac cgaaaagaga
     2581 ttacgctacc tccagctact ttcgatagcc gtctagccaa atacttgctt tccaccgttg
     2641 aggacaattc cctaacaacc attgccaacc tctatggtca gatcagtctt gttccagatg
     2701 aggcagttta tggcaagggt gctaagttag acttgccaga acgcgagatc tttttcccac
     2761 acttggcacg taaggttcag gttttgattg agacagaaga gccgatgttg accaaattag
     2821 aagaaaatca gcaattagat cttttgtttg acatggagtt gccactggca aatgttttag
     2881 ctaagatgga aattgctggt attaaggtgg aagctgagac tctcaaagct atgcaatctg
     2941 aaaacgaagt tttgattgac cagttgacca aggaaatcta tgaactagct ggtcaggaat
     3001 ttaatatcaa ttcgcctaag cagcttggga ccattctctt tgaagagatg ggactaccgt
     3061 tggaatatac caagaaaacc aagacaggct attcaacagc agtagatgtc ttagaacgat
     3121 tagcgccaat tgcaccagtt gtttcaaaaa tcttggaata ccgtcagatt accaaacttc
     3181 aatcaactta tgtagtcggc ttgcaggatg ccattttaga agatggtaag atccataccc
     3241 gctatgtgca ggatttgaca caaacaggtc gtctatcttc gacggaccct aacttgcaaa
     3301 atattcctgt tcgtctagaa caagggcgct tgattcgtaa ggcatttgtg ccatcgcttg
     3361 aaaatagtgt ccttttggca tcggactatt cacagattga attgcgcgtc ttggctcata
     3421 tttctcagga caagcatttg attgaggcct tccagcaagg tgcagatatt cacacttcga
     3481 cagctatgcg ggtcttcggt atcgaaaaag ctgaggatgt gacaccaaat gaccgtcgga
     3541 atgccaaggc tgtaaacttc ggtgtcgtct atggtatttc agatttcggt ctatccaata
     3601 acttggggat tacacgtaag gaagctaaag cctatatcga tacctatttt gaacgcttcc
     3661 caggaattaa gaattacatg gaaaccattg ttcgtgaggc acgtgataag ggttatgtag
     3721 agacgattta caaacgccgt cgtgaattac cagatataaa ctctcgcaat ttcaatgtcc
     3781 gtaattttgc ggagcgaaca gctatcaact caccaattca aggttcggcc gcagatattc
     3841 tcaaagtagc catgattaat cttgacaagg ctttgactga agcaggtctt gcgacacgta
     3901 tgttattgca agtacacgat gagattgttt tggaagtccc agtggcagag ctagaaacgg
     3961 taaaagccat ggtaaaagag accatggaat cggcgattag cctgtctgtg ccattgattg
     4021 cggatgaaaa tgaaggttca acatggtatg aagcaaagta agaaaggaaa aagtatgaaa
     4081 aaattaattc tttgtattgg tctatgtagt cttctacttg caggttgcgg aaaagcatcg
     4141 cagaagactg agaaaacacc agcttcttca ggggaagagt tttcaaccac cttaccgatt
     4201 ttgcaagaaa aacaggatac tacgaatgaa cagttcaatg ttttagcaaa tgcagaggtt
     4261 gtagctgtta atagtacacc acctgccgac gaaacacaaa aaggccacaa gatttatgcg
     4321 gccaataatg gtgttgctga aaaagatgaa aatgggaata tcaaggaata tttccgttat
     4381 tataatgtgc catctacttg gacaattaat gcagaaaata ctaaggatga gacggtcgct
     4441 gcagtctacg atgttaaaga ggggggaagc aattacatgg ttcaactcta taatatcaat
     4501 gcttttaatc agtctccatt ggaagacggt cgcaatatga cgccggagga attagaagca
     4561 cggatggttg aaacaaatca ttctttcaat gaaaagacgg ttgtcacaat caatggtcag
     4621 gaatggcggg ttgggcgaca gttattaaca gatcaaaaat tagctcgtct gactttttat
     4681 cggatggaat cgacagctgc ttatgacgac tctgtggttg tgggttcaat ctattattcc
     4741 ttagatccag gaaccgataa agatcggacc aatcttaaaa aggttatcgg gcaagttaag
     4801 gatgtcgtgt accaaattgc taaaaaataa tcaaggtaga caagctataa tcagtaacg
//
LOCUS       DBNFEG010000155         4817 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.155, whole genome shotgun sequence.
ACCESSION   DBNFEG010000155 DBNFEG010000000
VERSION     DBNFEG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.155"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..525
                     /locus_tag="AC1PNG_001774"
     CDS             <1..525
                     /locus_tag="AC1PNG_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HIP6702257.1"
                     /translation="LSLMPALIAVIIALVNGNLGAGVAVAGTFSLIKFRSAAGSSKEM
                     LAVLLAMAIGLATGMGFLGLAVFMTVILSACILIFENISFAQVNQNRRHLLVTVPMNF
                     DYDQFFENQFGKSCKQSDLLSLKYKKKKEALVLEYQVLLDKKITDKRLVDTVLSAGPL
                     DVVLNKQMPKKKYL"
     gene            complement(574..1056)
                     /locus_tag="AC1PNG_001775"
     CDS             complement(574..1056)
                     /locus_tag="AC1PNG_001775"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIP6702258.1"
                     /translation="MTKKPVQLATICYIDNGKEFLLLHRNKKENDVHQGKWIGVGGKL
                     EPGETPQACAIREIFEETGLTVTKHALKGVITFPDFTPNTDWYTYVFKITGFEGSLID
                     CNEGDLEWVPYDQVLSKPTWEGDRHFQEWLLEDKPFFSACFRYDGGKLLDYSVDFYEE
                     "
     gene            1223..1786
                     /gene="folE"
                     /locus_tag="AC1PNG_001776"
     CDS             1223..1786
                     /gene="folE"
                     /locus_tag="AC1PNG_001776"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIP6702259.1"
                     /translation="MSKQEQIEQTIYQLLELLGEDPNREGLLDTPKRVAKMYLEMFNG
                     LEEDPKDQFTAVFSEGHEEVVLVKDIPFHSMCEHHLVPFYGIAHVAYIPSKGRVTGLS
                     KLARAVEVASRRPQLQERLTHQVAHALQDALEPEGVFVMVEAEHMCMSMRGIRKPGSK
                     TVTTVALGKYKEDAILRRELLSMIHNK"
     gene            1795..2607
                     /gene="folP"
                     /locus_tag="AC1PNG_001777"
     CDS             1795..2607
                     /gene="folP"
                     /locus_tag="AC1PNG_001777"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIP6702260.1"
                     /translation="MSIEQLANQVTIMGILNVTPDSFSDGGHYNEVESALVQVEKLLA
                     EGATVIDVGGESTRPGATFVSEEDEIARVVPIIRAIKENYDCLVSVDTYKTGTARAAL
                     EAGADILNDVWAGLYDGDMLALAAEYKVPIILMHNQKDESYADVVGEVKNFLAERAQA
                     ALDAGVSADQIWLDPGFGFSKNVQHNLDLLQGLEQITALGYPVLFGISRKRVVDYLLG
                     GNSLPTDRDQATAALSAWAVQKGCKMVRVHNVAANRDIVKVWDQLTSGGQHG"
     gene            2600..2962
                     /gene="folB"
                     /locus_tag="AC1PNG_001778"
     CDS             2600..2962
                     /gene="folB"
                     /locus_tag="AC1PNG_001778"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIP6702261.1"
                     /translation="MDKISLNKCRFYGYHGAFKEEQVLGQIFTVDCDLFVDLTAASQT
                     DNLEDTVHYGMVFETIKAVVEGKPYILIEKVAGVICQELFDQFPKVEKIRLAIYKENP
                     PIAGHYDSVGIELERERP"
     gene            2959..3447
                     /gene="folK"
                     /locus_tag="AC1PNG_001779"
     CDS             2959..3447
                     /gene="folK"
                     /locus_tag="AC1PNG_001779"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIP6702262.1"
                     /translation="MKHLAYLSIGGNMGNRQAYLQAALDKLASHPGCQLGLVSNIYET
                     PAWGKTDQADFLNLACQVYTDLSAQDFLSACQNIEQELDRVRIEKWGQRTIDLDIIFW
                     DEEKIQEENLIVPHPYAHERAFVLNPLAEIASDYRHPILKKVVSDLLAELGEQKDIKI
                     FK"
     gene            3498..4808
                     /locus_tag="AC1PNG_001780"
     CDS             3498..4808
                     /locus_tag="AC1PNG_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HIP6702263.1"
                     /translation="MTILATLGVLLAIVAIIYWTSKNLHVIIAAPLASLIIILTNQMN
                     VLEVMLGKEQSYMAGLAGFLINNFAIFMLGSILARYMEASGATQTIANSILKVMGKDS
                     PYKGLLAITLIASILTYGGVSIFVVIFTLLPLSRPLFKELNINWALFPIPVFLGAGTY
                     TMTTLPGAPSIQNVIPTKVLGTSLTAAPVISLAASLTLLVFGLLYMAYCLKKSLANGE
                     TYTEEEDETAVVVADKTPNLFLSVLPLFSLIGTIFLMSKTPNVLAIGLLVSILLSALI
                     FYPYLPNQKEILNAATTASIVPAFATSSTVAFGTVLTLSAGFAVIQEWIQQIPGSPLI
                     SLSVSTALVSGIIGSSSGAVGIASSNFLPAYLEMGINPELLHRVVVVASAILTVVPQS
                     GVMITFHNLSKLSMKRGLKYSFILVTVGHLLALFVILLLAPLLY"
BASE COUNT         1313 a          936 c         1088 g         1480 t
ORIGIN      
        1 ctaagtttaa tgccggcatt gattgctgtt attattgcat tagttaatgg taacttagga
       61 gcgggtgttg cagtagcagg gactttcagt ctaattaaat tccgctcagc agcaggttct
      121 tcaaaggaaa tgcttgcagt cctcttagcg atggcaatcg gtctagcgac agggatgggc
      181 ttcctaggtt tggctgtctt catgacggtc atcctatctg cttgtatctt gatttttgaa
      241 aatatcagct ttgcccaagt caatcaaaat cgtcgtcatc tcttggtgac agtgccaatg
      301 aattttgact acgatcaatt ttttgaaaat caatttggta aatcttgcaa gcagtctgat
      361 ttattatccc ttaaatacaa aaagaaaaaa gaagcacttg ttttagaata tcaagtcctt
      421 cttgataaga aaattacaga taaacgtttg gtagatacag tattgtcagc cggtcccttg
      481 gatgtcgtat taaacaagca aatgccgaag aagaagtatt tgtaatatca aagaagccct
      541 ggatttccaa ggcttttttc gttttttatt agcttattct tcataaaaat caacactgta
      601 atcaagcagt ttgcccccat catagcggaa gcaagcggaa aaaaatggtt tgtcctctaa
      661 tagccattct tgaaaatgcc ggtcgccctc ccaagttggt ttggacaaga cttggtcata
      721 gggaacccac tctaagtcac cttcattgca gtcaataaga cttccttcaa aaccagttat
      781 cttaaagaca taggtgtacc agtctgtgtt tggagtaaag tctgggaagg ttatcactcc
      841 ttttagagca tgctttgtta cagtaagccc agtttcctca aagatttctc gaatggcaca
      901 agcctgtggc gtttcgccgg gttctagttt gccaccaact ccaatccatt ttccttgatg
      961 gacatcgttt tctttcttgt ttcgatgaag gagaagaaat tccttaccat tatcaatgta
     1021 gcaaatcgta gcgagttgga cgggtttctt ggtcatagag gctccttgta gttgatttat
     1081 cctattatac cacaaaggtg ggagtaggga cacctgcttg ttgctctgat aggggacagt
     1141 tttaggatta gcatactgaa agcccttttc tgaaaacagt ttttgaggta taataagaac
     1201 atcaaactat ttaggatgca ctatgtcaaa acaagaacaa atcgaacaaa caatttatca
     1261 attattagaa ttattgggtg aggacccgaa tcgtgaaggt ttattagata cgcctaaacg
     1321 ggtcgctaag atgtatttag aaatgtttaa cggcctagaa gaggatccca aagaccaatt
     1381 tacagctgtc ttttcagaag gtcatgaaga ggttgttttg gtcaaggata ttccttttca
     1441 ttctatgtgt gaacaccatt tggtaccctt ttatggcatt gcccatgtag cttatattcc
     1501 gagtaagggg cgtgtgacag gtcttagcaa attggcgcgt gcagtggagg tagcaagtcg
     1561 tcgccctcaa ttgcaggaac gcttgaccca tcaggttgcc catgcccttc aagatgccct
     1621 tgaaccagaa ggtgtttttg tcatggtcga agcagagcac atgtgcatga gcatgcgtgg
     1681 tattcgcaag ccaggtagta agacagtgac cacagttgct cttggcaaat acaaggaaga
     1741 tgccatcttg cgccgtgaac tcttgtccat gattcacaat aaataggagg ttctatgtca
     1801 atcgaacagt tagcgaatca agtaaccatt atggggattc tcaatgtgac gccagattca
     1861 ttttcagatg gtggtcacta taatgaggtg gagtctgcct tggtacaagt agaaaagttg
     1921 ttggctgagg gtgctaccgt cattgatgtt ggtggtgagt ccactcggcc aggtgcaaca
     1981 tttgtgtctg aagaagacga gattgctcgc gtggttccga ttatccgcgc tatcaaagaa
     2041 aactatgact gtcttgtcag cgtcgatacc tataaaacag gaactgcacg tgcagccttg
     2101 gaagcaggcg cagatatttt aaatgatgtc tgggcaggac tttatgatgg agatatgttg
     2161 gccctggctg cggagtacaa ggtaccaatc attcttatgc acaaccagaa agacgaaagc
     2221 tatgcggatg ttgttggaga ggtcaaaaac tttctagctg agcgtgccca agcagcactt
     2281 gatgctggtg tttccgcaga ccagatttgg ttggatccag gatttggctt ttcaaagaat
     2341 gtccaacata accttgatct cttacaaggt ttggaacaga ttactgctct tggttatccc
     2401 gttctatttg gaatatctcg taagcgcgtg gtggattatc tgcttggggg caatagctta
     2461 ccgactgacc gcgatcaagc aacggcagcc ttgtctgcct gggcagttca aaaaggctgt
     2521 aaaatggtcc gtgttcacaa tgtcgctgcc aaccgtgata tcgttaaggt ttgggaccaa
     2581 ttgacttctg gaggtcaaca tggataagat ttcacttaat aaatgccgtt tctacggcta
     2641 ccatggtgcc ttcaaagaag aacaagttct tggtcaaatt ttcacggttg actgtgattt
     2701 gtttgttgac ctgacagcag cttcacaaac tgacaattta gaagacacag ttcactatgg
     2761 tatggtcttt gaaaccatta aagcagttgt ggaaggcaag ccctacatcc ttatagaaaa
     2821 ggtagcaggt gttatttgtc aggagctttt tgatcaattt ccaaaggtgg agaagattcg
     2881 cttggccatt tacaaggaaa atccgcctat tgctggacac tatgattctg tcggaattga
     2941 attggagcgt gaacgaccat gaagcatcta gcctatctca gtatcggagg gaacatgggc
     3001 aatcggcaag cctatttaca ggcagctcta gacaaattag ccagtcatcc aggttgccag
     3061 cttggcttag tttccaatat ttatgaaact ccagcttggg gcaagaccga ccaggctgat
     3121 ttcctcaatc tagcctgtca agtgtatact gatttgtctg ctcaagattt cctaagcgct
     3181 tgccagaaca tcgaacaaga gttggaccga gtacggattg aaaaatgggg acaacggacc
     3241 attgatttgg atattatttt ttgggatgag gagaagattc aagaagagaa tctgattgtc
     3301 ccccatccct acgcccatga aagagcattt gttctcaacc ctcttgctga gattgcttct
     3361 gattaccgtc acccaatttt gaaaaaagtg gtatcggact tgcttgcaga actaggggaa
     3421 caaaaagata taaaaatttt caaatagttg cctctgatac taggtttatt agaggataga
     3481 atagataaag gagttttatg acaattttag caactttagg agttttattg gctattgtag
     3541 ccattattta ttggacatca aaaaatttac atgttatcat tgctgcacca ctagctagtt
     3601 taatcatcat tctgaccaat cagatgaatg ttttggaagt tatgttgggc aaagagcagt
     3661 cttatatggc gggcttagca ggttttttga ttaataattt tgccatcttt atgttggggt
     3721 ctatcttggc acgttatatg gaggcaagtg gagctacgca gacgattgcc aatagcattt
     3781 tgaaagtaat gggaaaagat agtccctata agggtttgtt agccatcact ttgattgctt
     3841 ccattctgac ctatggtggt gtgagtattt ttgtagttat ttttaccttg cttccccttt
     3901 cacgcccact ctttaaagag ttgaatatca actgggcact ttttccgatt cctgtctttt
     3961 tgggagcagg tacttatacg atgacaactc ttcctggggc accttccatc cagaatgtta
     4021 ttccgacaaa agttttggga actagcttga ccgcggcacc cgttatcagt ttggcagcca
     4081 gcttgacgct attagtattt ggcttgctct acatggccta ctgcctcaag aaaagtctag
     4141 caaatggaga gacgtataca gaagaagagg atgaaactgc agtagtggta gctgataaga
     4201 caccaaatct atttttatca gtcttgcctc tattcagtct tattgggacg attttcctaa
     4261 tgagtaaaac tccgaatgtc ctagcaatcg gattgttggt ctctatcctt ctatcagcct
     4321 tgatttttta tccatatctg ccaaatcaaa aagaaatttt gaatgctgcg acaacagcat
     4381 ccatagtgcc tgcttttgca acttctagta cagtagcatt tgggacggtg ttaaccttgt
     4441 cagcaggttt tgcagtcatt caagaatgga ttcaacaaat accaggatcg ccgctgatta
     4501 gtttatctgt ttcgacagca ttggttagtg gtattatcgg ttcatcttct ggtgcagtag
     4561 gtattgcttc aagtaatttc cttcctgcct atttagaaat ggggattaat ccagagttgc
     4621 tgcatcgtgt agtagttgtt gcatcagcta tcttgacagt tgtgcctcag tctggcgtga
     4681 tgattacctt ccacaatctt tcgaaattaa gtatgaaacg tggactcaag tattcattta
     4741 tcctagtgac agttgggcat ttgttagcct tatttgtgat tttactgttg gcacctttgc
     4801 tatattagga ttgatta
//
LOCUS       DBNFEG010000156         4782 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.156, whole genome shotgun sequence.
ACCESSION   DBNFEG010000156 DBNFEG010000000
VERSION     DBNFEG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.156"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            102..482
                     /locus_tag="AC1PNG_001781"
     CDS             102..482
                     /locus_tag="AC1PNG_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702264.1"
                     /translation="MSFINQFKSQSKTVLAVVAISAATLGFAGGMSISKSSTGVSTQS
                     QASLAQNSSAAMNGQGAPQGMPPGKGGPSQGMPPGQGTPPHDATSGATQSNSSTSSDE
                     VNALKKKNQEIKAQISGDNSSSNQ"
     gene            676..1347
                     /locus_tag="AC1PNG_001782"
     CDS             676..1347
                     /locus_tag="AC1PNG_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIP6702265.1"
                     /translation="MIKILLVEDDLSLSNSVFDFLDDFADVMQVFDGEEGVYEAETGV
                     YDLILLDLMLPEKDGFQVLKELREKGVTTPVLITTAKESLEDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRAGKFNENTLTFGDVTVDLSTNMTMVNGEEVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRIWGFDSDTTISVVEVYVSKIRKKLKGTTFGENLQTLRSVGY
                     ILKNA"
     gene            1340..2719
                     /locus_tag="AC1PNG_001783"
     CDS             1340..2719
                     /locus_tag="AC1PNG_001783"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIP6702266.1"
                     /translation="MPKRFKKLMYTDKFSFFIRYFAVFTLIFGLMTAIIFQLMRSTMY
                     QNSDNTLKRIKKEPALAVGFAIARTYEPNSVFILQDSPTGEETTSSSSDSTPVPKNQK
                     NTRAGDQLRLGANTHVLLYSKSGEMINPDTFTGLADLSLDKEKLGEIKETTVESSFGM
                     SEDYRYITIGLATDELGYYSSYDIKYATILVNVSQIKSSIESYESTVAIVMVSAWLIS
                     ILASIYLSNLSMRPILISYQKQKDFVENASHELRTPLAVLQNRLESLFRHPETTILES
                     SESIGSSLEEVRNMRLLTTNLLNLARRDDGLKVDMIDVQPNYFDEIFANYLMIAEENG
                     KTLTVNNLIHQPIRTDKVLIKQLLTILFDNAMKYTDDDGTIQMAANIKDKLVCFTVID
                     NGLGISDADKKKIFDRFYRVDKARTRQKGGFGLGLSLAQQIIKNLDGEISVRDNQPKG
                     TIFEVRLPK"
     gene            3043..4272
                     /locus_tag="AC1PNG_001784"
     CDS             3043..4272
                     /locus_tag="AC1PNG_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIP6702267.1"
                     /translation="MRKVSRGVLTILLSATVLSACSGGGQELSERLEAAKIEFSKEKK
                     QLEAELTALKEEVQGNFYYQQLDTDKERRVYLQFVNGLSKRMQVIDIDSVNDEIYSRV
                     YFSVAHDYPEFYWLTDEAAMAGGIDILDLEEPVYPSDLSPTQNKIEDEVNKILAQTPK
                     GSDYEKVKYFYEVIIKQTDYDLEALQTKSVTWRSQGITSVLLDKKSVCAGYSRTFQYL
                     CKKAGIDCIYVTGIAKNGQNGEFGHAWNLVKINGQYYGVDTTWGDPVFDQAISGEAHT
                     DISYDYLCVPDEILERSRIADSDLLDYWGEEQYYEPRVLVYPKCTDNSLNYYVQKGVY
                     FTSFDEAAVLQSITDQRLQGTNKVVLQFGTAEAMQQMITLASTENNAIFQALGDVGEY
                     QYYYNDQTYTFELADWF"
     gene            complement(4534..4782)
                     /gene="rpsT"
                     /locus_tag="AC1PNG_001785"
     CDS             complement(4534..4782)
                     /gene="rpsT"
                     /locus_tag="AC1PNG_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIP6702268.1"
                     /translation="MEVKPLANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANP
                     SEELFRAASSAIDKAETKGLIHKNKASRDKARLASKLA"
BASE COUNT         1532 a          795 c          988 g         1467 t
ORIGIN      
        1 agcttgtttt aagttttcaa tactatacta ttatcatcag gtgaagagga gttatttaac
       61 aggtctcatt ctgataatat tctattgaaa aggggaattt catgtcattt attaatcaat
      121 ttaagtcaca gtcaaaaaca gtactagctg ttgttgctat ttcagctgct accttgggct
      181 ttgcaggggg aatgagtatt tcgaaaagct caacaggagt ttcaacacaa agtcaggcaa
      241 gtttggcgca aaatagttct gcagcaatga atggacaagg agctccccaa ggcatgccac
      301 caggtaaagg aggtcctagc caaggtatgc cacctggaca aggtacaccc cctcatgatg
      361 caacaagtgg agcaactcag tcaaactcga gtacttcttc tgatgaagtg aatgctttaa
      421 aaaagaagaa tcaagaaatt aaagctcaaa tttcaggaga taatagttct tctaatcaat
      481 aaatattgag atttcgattt ttacttattc catgatataa tgaactcgta tcaataaata
      541 gttagataaa gactacctta tcattcattt ttcagatggt catagtagta ctttatctaa
      601 ccatttctct taaagaccgt gacctgtccc aggtctttgg tttgtttatt aaaccgaaac
      661 gtaaggaagt gtatgatgat taaaatattg ttagtggaag atgatttaag tctatcaaat
      721 tctgttttcg atttcttaga tgattttgcg gatgttatgc aggtttttga tggtgaagag
      781 ggcgtttatg aggcggaaac aggcgtttat gatttgattc ttttggattt gatgttgcct
      841 gaaaaagatg gcttccaagt cttgaaagaa ttacgtgaaa aaggtgttac gacacctgtt
      901 ctgattacaa cagccaagga aagtctagag gataaaggcc atggttttga attgggggct
      961 gatgactatt tgacaaaacc gttttattta gaagaattga agatgcgaat tcaagctcta
     1021 ttaaaacgag cgggtaagtt taatgaaaac acactaacct ttggagatgt gacagttgac
     1081 ctatctacaa atatgacgat ggtaaatggc gaagaagttg aactgctagg aaaagaattt
     1141 gatctactag tttatttctt gcaaaatcaa aatgttattt tgccaaaaac acaaattttt
     1201 gaccgtattt ggggctttga cagtgatact acaatttcag tagtagaagt atatgtttct
     1261 aaaattagga aaaagttgaa aggaacgacc tttggggaaa atcttcaaac cctgcgtagt
     1321 gtcggataca ttctaaaaaa tgcctaagcg atttaaaaaa ttaatgtaca cggataaatt
     1381 ttctttcttt atccgttatt ttgcagtatt tactctgatt tttggtttaa tgacagccat
     1441 catttttcaa ttaatgcgtt caactatgta ccaaaattca gataacacct tgaaacgcat
     1501 taagaaagaa ccagcgctgg ctgttggttt tgcaatcgca agaacctatg agcccaattc
     1561 tgtatttatc cttcaagata gtccaactgg tgaagaaact acgagctcta gttcagatag
     1621 cacgcctgtt cctaaaaatc aaaaaaatac tagagctgga gatcagctga ggttaggtgc
     1681 caatactcat gttttactct atagtaaaag tggagagatg attaatccag atacttttac
     1741 tggtttggcc gacctatcat tagacaagga aaaattgggt gaaattaagg aaactacggt
     1801 tgaatcaagt tttggtatgt ctgaagatta tcgctatata acgattgggc tggctacaga
     1861 tgaactaggg tattattcgt cctatgatat aaaatatgcg acaattttgg tgaatgtcag
     1921 ccaaattaaa tcttccattg agagctatga atcgacagtt gctattgtta tggtatcagc
     1981 ttggttgatt tctatactag caagtattta cctatcgaat cttagcatgc gtccaatcct
     2041 aattagttat caaaaacaaa aagacttcgt tgaaaatgct agtcatgagt tacgcacacc
     2101 gttggcagtt cttcaaaatc gcctggaaag tctatttcgt catcccgaga caactatttt
     2161 ggaaagtagt gaaagcatcg gatctagttt agaagaagtt cgaaatatgc gactattgac
     2221 aacaaattta cttaatttgg ctcgtcgtga tgatggctta aaagtcgata tgatcgatgt
     2281 ccaacccaac tattttgatg aaatctttgc taattacctt atgattgccg aagaaaatgg
     2341 aaaaactcta acagttaata atttgattca tcagccaatt cgaacagaca aagttttgat
     2401 taaacagttg ctcaccattt tgtttgacaa cgctatgaag tataccgatg atgatgggac
     2461 aattcaaatg gcagctaata tcaaggataa actagtttgt tttacagtta ttgacaatgg
     2521 tttgggaatc agcgatgccg ataaaaagaa aatctttgat cgcttttacc gagttgacaa
     2581 ggctagaaca cgtcaaaaag gtggatttgg tttaggttta tctttagctc aacaaattat
     2641 taagaattta gatggtgaga tttctgtgag agataaccaa ccgaaaggaa ctatttttga
     2701 ggtacgttta cccaaataac tgaccattca tcaagattag aggctgggta aaaaatccag
     2761 cctttcttct caaagttcgc tctatttcca acttccaact gtctcccaga tagttttgta
     2821 gcgaactctg ttcgctctat ctccagcctc caaaggtttg gggaactgtt tcagctggtc
     2881 acctaaaaat aagaaagtga ccatagaaaa tcgaacctaa atgtttggaa gtgaggggaa
     2941 ttctttttcg atctattgaa ttctttccac tccctttttg ttttgtgtag tttgtcagat
     3001 gcctcctaat tatgttatac ttaaacaaga atggaggtaa atatgaggaa agtttctcgt
     3061 ggcgttttaa ccatattatt atcggcgaca gtcctttctg cttgttcagg aggaggacaa
     3121 gagctgagtg aacgtttgga agcagctaaa attgaatttt ctaaagaaaa aaagcaacta
     3181 gaagcagaat taacagcatt aaaagaagag gttcaaggaa atttttacta ccaacaattg
     3241 gatactgata aggaacgtcg tgtctatctc caatttgtta atggactcag caagcggatg
     3301 caagtgatcg atattgattc tgttaatgat gaaatatatt cgcgcgtata tttttctgtc
     3361 gcccatgatt atccagaatt ttactggcta acagatgaag cagcaatggc tggtggaatt
     3421 gatatattgg atttagaaga gccggtgtat ccgagtgatt tatctccgac acaaaataaa
     3481 atagaagatg aagtgaataa gatacttgct cagacaccaa aagggtcgga ctatgagaaa
     3541 gttaaatatt tttacgaggt gattataaag caaacggatt acgaccttga agccctgcag
     3601 accaaatctg tcacgtggag aagtcaagga attaccagtg tgctattaga taagaaatcc
     3661 gtttgtgctg gctatagtcg caccttccaa tatctatgta aaaaagcagg cattgattgt
     3721 atctatgtca cagggattgc aaaaaatggg caaaacggtg agtttgggca tgcttggaat
     3781 ctagtcaaga taaatggaca atactatggt gttgatacga cttggggaga tccagttttt
     3841 gatcaagcta taagtggaga agcgcataca gatatttcat acgattatct atgtgtccct
     3901 gatgaaatat tagagagaag tagaattgct gatagcgacc tactcgacta ctggggagaa
     3961 gaacaatatt acgaacctag agtattggtc tatccaaagt gtacagataa ttcattgaat
     4021 tactatgttc aaaagggagt ttatttcaca agctttgatg aagctgcagt tttacagtcc
     4081 atcactgacc agcggctaca aggtacgaat aaagtagtct tgcaattcgg aacagcagaa
     4141 gcaatgcaac aaatgattac attggcctct actgaaaata atgctatttt tcaggctttg
     4201 ggcgatgtag gagagtatca gtactattat aatgaccaaa cctatacatt tgaactggca
     4261 gattggtttt aaggaattag tttgaatctc taggagcgtg ttcttcccta ataataaaaa
     4321 atagggagcg ggaaagacat ccatggtggc tccgccctca cccacagaat aatgaaaaca
     4381 accacaaagc cctcactagg tgtataatca tagtgagggc tttgtggttg acgagttaat
     4441 gatgttagtc atcgcgggaa aactgatcca ctttgtcccg cctccttatg atggaaaaaa
     4501 taaaaaaagg aaacccaaag gtttcttttt taattaagca agttttgatg caagacgtgc
     4561 tttatcgcgg cttgctttgt ttttgtgaat caaaccttta gtttctgctt tatcgattgc
     4621 tgaactagca gcacggaaaa gctcttcaga tgggttagct tcaaatgctt tgattgcagt
     4681 acgcatagct gatttttgtg ctgagttttt ctcgttttgt ttaacgttca attcagcgcg
     4741 tttgatagct gacttaatgt ttgccaatgg tttcacctcc ac
//
LOCUS       DBNFEG010000157         4758 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.157, whole genome shotgun sequence.
ACCESSION   DBNFEG010000157 DBNFEG010000000
VERSION     DBNFEG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.157"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            255..1412
                     /locus_tag="AC1PNG_001786"
     CDS             255..1412
                     /locus_tag="AC1PNG_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIP6702269.1"
                     /translation="MLKTKKAKIALFGGLGVVAVALVGGALIFSNMLTSPSSSEDVAM
                     SMTYTVAKEGSIASSTLLTGTITAADEQYVYYDPSKGDLSEVLVAPGAKVEVGTPLIR
                     YDATELQSALDTAVRGRDKIGRQIADLRTNGQTVQTTGDAETDAAATAAAQRSVDMQL
                     ADLNDSYADAQAAVDKAYTALTEATVTSTVAGTVVEVNKAVSKNSTSSQTVVHIVNQG
                     SLQVTGSLTEYDLANIAVDQEVKLTSKVYPDKTWTGKITYISNYPSSNQAGAATTGTS
                     GSGAKYPFKAALTSELGELKQGFSVNIEVVNTSKNILIPVSAVVPEEDKNYVWTLVDG
                     KAKKVEVTLGNADALNQEVTAGIAAGDKVITIPTPNLEDGKEVEANEEPAY"
     gene            1393..2088
                     /locus_tag="AC1PNG_001787"
     CDS             1393..2088
                     /locus_tag="AC1PNG_001787"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6702270.1"
                     /translation="MKNQLIRLTSINKSYKNGDQELRVLKNIDLEVEEGEFLAIMGPS
                     GSGKSTLMNIIGLLDRFSSGNYWLEGEEVSQLSEKKLAQVRNDQIGFVFQQFFLLSKL
                     NALQNVELPLIYAGVPSSKRKELAKQYLEKVELAERMTHLPSELSGGQKQRVAIARAL
                     VNTPAIILADEPTGALDTKTGEQIMQLLTELNNEGKTIIMVTHEPEIAAYAKRKIVLR
                     DGVITEDSRREGV"
     gene            2089..3336
                     /locus_tag="AC1PNG_001788"
     CDS             2089..3336
                     /locus_tag="AC1PNG_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6702271.1"
                     /translation="MENWKFALKSIMAHKMRSLLTMLGIIIGVAAVVIIVAMGTGVAK
                     SIEKSLAGDQNNVQVYFTAFSSSGTAFGPFGTASTEEPILEEEPDLTDSTLKGLLEID
                     GVSNYYSSISNIAEVRSGNKKAENVTITGVSQNFFAVKEYEILAGRQFTANDYSRFSR
                     MIMLDTALAEKLFGSIENSLNQTISVNSNAYLVIGVYKDPKAGTAIYGMNSGGNAVMT
                     NTQLAAETGMKENSQIFVHVEDVTRATEVGKAAADYLTKATGLKEARYEIYDMSAMLE
                     EFKSQMSGVTLFIGAVAGISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRGNILI
                     QFLIEAMVLTTLGGAIGLAIAQTIVFLLNVSKALGERIAAEISIPVVLGSLAFSAVVG
                     IVFGVLPANKASKLDPIEALRYE"
     gene            3462..4526
                     /locus_tag="AC1PNG_001789"
     CDS             3462..4526
                     /locus_tag="AC1PNG_001789"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIP6702272.1"
                     /translation="MVELGISTFGETTPLEKTGETYSHDERIHQLVKEIELADAVGLD
                     VYGIGEHHREDFAVSAPEIVLAAGAVNTKHIKLTSAVSILSSMDPVRLYQQYTTIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLHDYEELFDEKLDMLLEIDKETNLKWDGHFTQSV
                     DNKPVYPRPVQEDFPIWVATGGNVESTIKIAKKGLPIVYAVIGAGAHRFKPLVDAYRK
                     VARNSGHDPKKTKVAAHSWGWIADDHDKAVEEYYHPTKVITDNIAKDRPHWSEMTKAQ
                     YLYSLTDEGATIVGDPKRVAEKIIKTIETLDLDRFFLHLPIGSMPHEDVLRAIELYGK
                     EVAPIVRDYFAKKEENQSQD"
BASE COUNT         1440 a          888 c         1097 g         1333 t
ORIGIN      
        1 caaattgaac aaattattct agctcataat ttacgctatt ttgaaatgaa ctagcaccac
       61 gggttaacta tattcattgt aacgcttttt tgtgtgtcaa tcaaaaatcc gctacgttac
      121 cttacaaaaa tgtaagtggg aaaattgccg aactttttat aaaatagtta ggaaaaaact
      181 tgcatttgtt cttgtatttg tgtattatat agacaacaca aataggtatg ttttgtaaag
      241 aaaaggagtt tgttatgtta aagacgaaga aagcaaaaat tgctttgttt ggtggtcttg
      301 gggtagtcgc agttgcctta gtaggtgggg ctttgatttt tagcaatatg ctaacatcac
      361 catcatcttc agaagatgta gccatgagta tgacctatac tgttgctaaa gaaggatcaa
      421 ttgcttcctc aacgctactt acaggaacga ttacagcagc agacgagcag tatgtttact
      481 atgatccttc taagggagat ttgagtgagg ttttggttgc tccaggtgct aaggtcgaag
      541 taggtacccc tttgattcgc tatgatgcta cagaattaca gtctgctttg gatactgctg
      601 ttcgtggtcg tgataagatt ggtcgccaaa ttgcagactt aagaaccaac ggtcagactg
      661 ttcaaacaac tggtgatgca gaaacagatg ctgcagcaac tgctgctgca caacgttcag
      721 tagatatgca gttggcagac ttgaatgatt catacgcaga tgctcaagct gcggttgata
      781 aagcatatac agccttaaca gaagcgacag taactagtac cgttgctggt acagttgttg
      841 aggtgaataa ggctgtttct aagaattcta catcgagcca aacagttgta cacattgtaa
      901 atcaaggtag tttgcaagta acaggtagtt tgacagaata cgatttggct aacattgcag
      961 tggatcaaga ggtcaagctg actagcaagg tatatcccga taagacttgg acagggaaaa
     1021 ttacctatat ttcaaactat ccatctagta atcaagctgg tgctgctaca acaggaacca
     1081 gtggttcagg tgccaaatat ccatttaagg cagctttaac tagtgaatta ggtgagttga
     1141 aacaaggatt ttctgtaaat attgaggttg tcaatacaag taaaaacatt ttaattccgg
     1201 tgtccgcagt tgtgccagaa gaagataaaa actatgtgtg gaccttggtt gatgggaaag
     1261 ctaagaaagt agaagttact ctggggaacg cagatgcttt gaaccaggaa gtgaccgctg
     1321 gtatagcagc aggagataag gttattacca ttccaactcc gaatctcgaa gatggcaaag
     1381 aggttgaagc caatgaagaa ccagcttatt agactaacaa gtatcaataa atcctacaaa
     1441 aatggtgatc aagaactccg agttttaaag aatattgatt tagaggtgga agagggagaa
     1501 tttcttgcca ttatggggcc ctctggttca gggaaatcaa ccttgatgaa cattattggg
     1561 cttttggatc gtttcagttc aggaaactat tggctggaag gtgaagaagt cagtcaactt
     1621 tctgagaaaa aattagctca ggttcggaat gaccaaatcg gtttcgtctt tcaacaattt
     1681 ttcctcttat cgaaacttaa cgcccttcaa aatgtagaat tacccttgat ttatgctgga
     1741 gttccatcaa gcaaacgtaa ggaactagca aaacagtatc ttgaaaaagt tgagttggca
     1801 gagcggatga cgcatttacc atcagaattg tctggtggtc aaaaacaacg ggtagctatt
     1861 gctcgtgcac tagtcaatac cccagctatt attttagcgg atgaacctac aggggctcta
     1921 gataccaaga caggtgagca gattatgcaa cttctgacgg agttgaataa tgaaggaaag
     1981 accattatca tggttacgca tgagcctgag attgctgcct acgcaaaacg aaaaatcgtt
     2041 ctacgtgatg gagtgatcac tgaagatagt cgaagggagg gagtttagat ggaaaattgg
     2101 aaattcgccc tcaaatctat tatggctcat aaaatgagat cgctccttac tatgttaggt
     2161 atcatcattg gtgtggctgc agttgtcatt attgtcgcca tgggaacagg ggtggccaaa
     2221 agcatagaaa agtctctagc tggtgatcaa aataatgttc aggtttactt tactgctttc
     2281 tccagttcag gtacagcttt cggtccgttt ggcacagcgt cgacagaaga accaatttta
     2341 gaggaagagc cggatttgac ggattctact ttaaaaggac tgctagagat tgatggcgta
     2401 tcaaactatt actccagcat cagcaacata gctgaagtca gatcgggcaa taaaaaagca
     2461 gagaatgtca ctattactgg tgttagccag aatttctttg ctgtgaaaga gtatgagatt
     2521 ttagctggtc gtcagtttac agctaacgac tatagtcgtt tttcacgtat gattatgtta
     2581 gatacggcat tagcagagaa gttatttggt tctatagaga actctctcaa tcagacgatt
     2641 agtgttaata gcaatgccta tctagtaata ggggtctaca aagatccgaa agcaggcaca
     2701 gctatttatg gaatgaattc aggtggaaat gccgtcatga ctaatacgca gctagctgct
     2761 gaaacaggca tgaaagaaaa ttctcaaatc tttgtccatg ttgaagatgt tactcgggca
     2821 acagaagttg ggaaggctgc tgcagattac ctgactaaag cgactggatt gaaagaagcg
     2881 agatacgaga tttatgatat gtcagctatg ttggaggagt ttaaatctca aatgtctggc
     2941 gttacattgt ttatcggtgc tgttgcaggt atctccttgc tagttggtgg tatcggtgtc
     3001 atgaatatca tgctggtatc agtaactgag cggactcgtg aaatcggtct tcgaaaagcg
     3061 cttggagcaa ctagaggaaa tatcttgatt caattcttga tagaagccat ggtcttgacc
     3121 actcttggtg gagcaattgg tctcgcaatc gctcagacaa tagttttctt gctcaatgtg
     3181 agcaaggcgt tgggcgaaag aatagctgct gaaatttcaa ttcctgttgt gttaggtagt
     3241 ctggctttct cagcagtagt tggtattgta tttggcgtac tacctgccaa caaagcatct
     3301 aagcttgatc caattgaagc tttgcgctat gaataatctg ttgaaacgat taggttttcc
     3361 tagtcgtttt attttttgag caaatcaatt gattttatca ctaattagag ataaaataaa
     3421 cgcatcaatt aagaaatagt gatttcaaag gagaacacat catggtagaa ttaggtattt
     3481 caacatttgg cgaaacaact ccactagaaa aaacagggga aacttacagc cacgacgagc
     3541 ggattcacca gctagtcaaa gaaatcgaac tagctgatgc ggtggggctt gatgtttatg
     3601 ggattgggga gcatcaccgc gaagattttg cggtttctgc acctgagatt gtgctggcag
     3661 ctggtgccgt caataccaag catatcaaac taacatcagc agtttcaatc ctttcctcca
     3721 tggaccctgt aaggctctat cagcagtaca ccaccattga tgctttgtca aatggcagag
     3781 cggaaatcat ggcgggtcgt ggttccttta cggagtcatt ccccttgttt ggctatgatc
     3841 tccacgacta tgaagaactc ttcgatgaaa agctagatat gcttctggaa atcgacaagg
     3901 aaaccaatct caagtgggat gggcatttta cccagtcagt cgataataag ccagtctatc
     3961 cacgtcctgt tcaggaagat tttccgatct gggttgcaac tgggggcaat gtagagtcca
     4021 ccatcaagat tgctaagaaa ggcctgccaa tcgtgtatgc agtcattggt gcaggtgccc
     4081 atcgctttaa acctttagtc gatgcttatc gcaaggttgc tcgcaactca ggtcatgatc
     4141 caaagaaaac gaaagttgca gcccattctt ggggttggat tgcagacgac catgacaagg
     4201 ctgtggaaga atactatcat ccaactaaag taattacgga caatattgcc aaagaccgcc
     4261 cacattggag cgagatgacc aaggcgcaat atctctactc tctgacagac gaaggagcaa
     4321 ccatcgtcgg agatccaaaa cgagtcgctg aaaaaattat caagactatt gaaacacttg
     4381 acttggaccg tttcttcctc catctcccaa tcggctcaat gccacatgaa gatgtcctcc
     4441 gtgctatcga actctacgga aaagaagttg cgccaattgt ccgagattat tttgcaaaaa
     4501 aagaagagaa tcagtcccaa gactgatgag ataaaaagga ggggacgtta taatagcctc
     4561 acttctctaa agtggagtca acatctcagc gcagtggttg attggaagat ttgttcgtgt
     4621 tttacactcc acatcttcct aatcaactgt gcgggggtgg gaagacgaac tctctatgat
     4681 tatttgagtt ctgtcccact cccaatccta aatatttttc ataatctcct gaaaatcctg
     4741 accatatggt tgggattt
//
LOCUS       DBNFEG010000158         4750 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.158, whole genome shotgun sequence.
ACCESSION   DBNFEG010000158 DBNFEG010000000
VERSION     DBNFEG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.158"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            124..3108
                     /locus_tag="AC1PNG_001790"
     CDS             124..3108
                     /locus_tag="AC1PNG_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7604 domain-containing protein"
                     /protein_id="HIP6702273.1"
                     /translation="MFGKRWLSIFAILLLTLSNCSPTIGYALENMSESTSSSVISSAE
                     ETVSKSETTTEMSQSSEEIVEMKQAEENTETNRSDAPSSSQDLADNSEESVQKILHYE
                     DEYVTADLNAYFGNGVENFSVTLPRMKNSGKWDSEREYVNQLSQYLVNQNLQPADVQQ
                     IEISDSQIVKEGYKPRLTVNFKTSLGLRGGLVTKENLRLVSTNTDGSLVFHDAEFHFD
                     TTGTLIGLTYEGILGKDIHVVMVKPLAEKTTVTLGQYVQMQLDGYVLEEEEEQTASNV
                     PLNARLKVKIFRSDASGIFTILSPIDSFREEKTFDVLSTWKPSFNLLGIESEKFDIVK
                     MNYRQAHQSKTDSLMYFNGEFPVASFHQESGEEESNVLEIYLQNRMLEGTGMILGLRT
                     RMLGYRSFSGFRSVTNATVSNSSVDQAHITQSKRIDYLGDGGNNPDTSVDDRIGTDTH
                     ELYRMYLDMTGTKEPFDTLIVVDRSTSMTDPMNSVDTQARYLAVYKALNGTAGRQGLL
                     SKLVGFHPENQVAIVGFQGYPGYPSGDQDSTVIANWGRSTSVALSNIQPPYNNGTNYT
                     AGLRTAGVVLDQNQSSRKKVMIFISDGVPTFAFVNGVRYGNGTISGNNPYYTRDWTLN
                     YFNSWIGKYPKLPIYTLGISSEFGNSDNLSANPYVLNHMSSQTGGFYSHVADSQALER
                     TLQKIVDDTKLSLVSINDQLSQYVSYYAEQPDVKVVQTNRQTGQVTTLYQNGAVTSQG
                     VGILQSVTHTAVNASDSTGKVDLNFVDNFKIDDTYTYTISYNVKVTDRAYEEKKTSGY
                     NAHGDSGTDYTGNSTSSGQAGFRSNKVASLTYIRNEQPYEKEYDHPVVQVDTRQFTLQ
                     KSDSKETNLLLENVRFELRLSDKKTVFASGETDSRGNITFTDLRRAKTYYLYETKAKD
                     GYTLPEDPWTVDVAVDGTITIRDQLGYTVSKSGEQYRVVNHKLYQLPSTGGYGPAVYI
                     LLGVMVITLASYLFYEKRKQIA"
     gene            3135..>4750
                     /locus_tag="AC1PNG_001791"
     CDS             3135..>4750
                     /locus_tag="AC1PNG_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HIP6702274.1"
                     /translation="MKRIFTLLTVLLTVLGMNARPVFAASNTDQAELKITNIEGNPTV
                     TLYKIGEGVYNATNDSFIRFDYASGVKLTETGPTAEEITNIANGILDNTIQAKASKVL
                     QVTNGTATYRATGAGVYIAILTGATDGRSFNPILLAASYNGEGKLTGGSVDSKAKYLY
                     GQTSVAKSTLPSIDKTVSGVTADADKNSASLGQVLTYSLNVTLPSYPTQAKNKTIFIS
                     DTMSEGLTFDYASLTISWNGQTLIADTTGQFKTQGDILIAKAVKVGNGFNLAFVYDNL
                     NEIAPVVTYKGIINDKAVVGGTGNANKAEFFYARNPNSGNTYEDPNNKPNPQNDNTIV
                     NKEDSETVYTYQIAFKKVDSITKAPLAGAVFGIYSDAETKQLVDIVTTNADGYAISTK
                     VGKGTYYLKEITAPTGYSLNTNVYTAEASWTSATTTSSASSTRSEYTSVVGEAKDSTQ
                     VGWLKHNVFYKLDNKPEGTDVQAAYLKATTTTAQNTTVTTTNPTAGAGTVSIADVPNT
                     KLGELPSTGGMGTYLFTFIGVLVLTVGLGL"
BASE COUNT         1538 a          809 c         1037 g         1366 t
ORIGIN      
        1 attttttagg acaggattga cgtttagaag agaattgtga tatagtacca aaagattctt
       61 tgctaaatca tttgattaat atagcaaatt tcgattctta tggaaaggag gaatatgaaa
      121 tttatgtttg gaaaaagatg gctttccatc tttgcgattt tgttacttac tttgagtaac
      181 tgcagtccaa caattggtta tgcgttagag aatatgagtg agtcaacaag tagttcagtt
      241 atttcttcag cggaagaaac tgtttctaaa agtgaaacaa cgacagaaat gagtcaatca
      301 tcagaagaaa ttgttgaaat gaaacaagca gaggaaaata ccgaaacgaa tcggtcagat
      361 gcacctagtt cttctcagga tttggcggat aattctgaag aatctgttca aaaaatcctg
      421 cactatgaag atgagtatgt gactgctgat ttaaacgcat actttggtaa tggtgttgaa
      481 aactttagcg taactctacc tcggatgaaa aactcgggta agtgggattc tgaaagagag
      541 tatgtaaatc aacttagtca atatctcgtg aatcagaatt tacagcctgc tgatgttcag
      601 cagatagaaa tttcagatag tcagattgtc aaggaaggtt ataaaccaag gttgacagtt
      661 aatttcaaga cttctctggg attgcgtggc ggtttagtga ccaaagaaaa tcttcggttg
      721 gtttcgacca atacagatgg ctccttggtt tttcatgatg ctgaatttca ctttgatacg
      781 acaggtacct tgataggatt gacctatgaa ggaatacttg gaaaagatat tcatgtggta
      841 atggttaagc cattagcaga aaagacaaca gtgacattgg gacaatatgt tcagatgcag
      901 ttagacgggt acgttctcga agaggaagaa gaacaaacag ctagtaatgt tcctttaaat
      961 gcacggctaa aagtgaaaat ttttcgttca gatgcttcag gtatctttac tatcttaagt
     1021 ccaatagatt catttcgaga agaaaagaca tttgatgtct tatccacttg gaaacctagt
     1081 tttaatttat tgggaattga gagcgagaag tttgatatag tcaaaatgaa ttaccgtcag
     1141 gctcatcagt ctaaaaccga tagcttaatg tatttcaatg gcgaatttcc tgttgcatca
     1201 tttcatcaag aaagtggtga agaagagagt aacgttctag aaatttatct ccagaaccga
     1261 atgctcgaag gtactggtat gattttgggg cttcgcacga ggatgttagg gtacagatct
     1321 tttagtggat ttagaagtgt aacgaatgcg actgtatcga attcgtcggt agatcaggcc
     1381 cacatcacgc agtctaaacg gattgactat ttaggtgatg gtgggaataa tccggatact
     1441 agtgttgatg accgtattgg tacagatacg catgaactct atcggatgta tctagatatg
     1501 acggggacaa aggagccctt tgatactcta attgtggtag ataggtcaac cagtatgact
     1561 gatccgatga atagtgtaga cacacaggct cgctatctag ccgtttataa agccttaaac
     1621 ggaactgcag ggcggcaagg gctgcttagt aagttggttg gttttcatcc ggaaaatcaa
     1681 gtagcaatag ttggttttca aggttatcca ggttatccaa gcggagatca ggattcaacg
     1741 gttattgcta actggggacg ttctacctcg gttgcactga gtaatattca gccaccttat
     1801 aataacggaa ccaattacac ggctggttta cgaacagctg gtgttgttct agatcagaac
     1861 caaagtagtc ggaagaaggt aatgattttt atttcagatg gggtgccgac ctttgcattt
     1921 gtgaacggtg ttcgatacgg aaatggtacc attagcggaa ataatcctta ttatacgagg
     1981 gattggacgc ttaactattt caacagttgg ataggtaagt atcctaagtt acctatctat
     2041 accttgggga tttctagtga gtttggaaat agtgacaact tgtctgctaa tccatatgta
     2101 ttgaatcaca tgtctagtca gacgggagga ttttattcgc atgttgccga ttcacaagca
     2161 cttgaacgga ctctccaaaa gatagttgat gatacaaaat tgtctcttgt ttccatcaat
     2221 gaccagttaa gtcaatatgt atcttattac gcggaacagc cggatgtgaa agtggtacaa
     2281 acgaatcgtc agacgggcca ggttacaacg ctctatcaga atggagcggt aacgagccag
     2341 ggagtaggta ttttacaatc agtaacacat acggctgtta atgctagtga ttctacaggg
     2401 aaggttgatt tgaattttgt agataatttt aaaattgatg atacctatac ttatacaatt
     2461 tcttataatg taaaagtgac agatcgtgct tatgaggaaa agaagacgtc tggttataat
     2521 gcacacgggg attctggaac tgattatacg ggaaacagta ccagttctgg gcaggctggg
     2581 ttccgatcta acaaggtggc atcattgacc tatattcgaa atgaacaacc ctatgaaaaa
     2641 gaatacgacc atcctgtggt gcaagtagat acacgtcaat ttaccctaca aaaaagtgat
     2701 agtaaggaga ctaatctcct gctagaaaat gttcgttttg aattacggtt atctgacaag
     2761 aagacagtct ttgcaagcgg tgagacagat agtaggggaa atataacctt tacagattta
     2821 cgtcgagcaa aaacgtacta tctatatgaa accaaggcta aagatggcta tactctccca
     2881 gaggatcctt ggacggtaga cgttgccgtt gatgggacga ttaccatcag agatcaacta
     2941 ggttatacag taagtaaaag cggtgaacaa tatcgcgttg tgaaccataa actttatcaa
     3001 ttaccatcta caggtgggta tggtcccgcg gtctatatcc tactaggtgt gatggttatt
     3061 acattagcaa gctatctttt ttatgagaag agaaagcaga ttgcttaatt ttttaaagct
     3121 caaaggagaa gaaaatgaaa agaatattca ctcttttaac agttctttta accgttttag
     3181 gaatgaatgc acgtccagta tttgctgcat caaatacgga tcaagcagaa ttaaaaatta
     3241 ccaacattga gggaaatcct acagttaccc tttataaaat cggtgaaggt gtatataatg
     3301 caacgaatga ctcgtttatt cgtttcgatt atgcaagtgg agttaaacta acagaaactg
     3361 gaccaactgc agaagaaatt acaaacattg ctaatggtat tttagacaat acaattcaag
     3421 ccaaagcatc aaaagttctc caagttacaa atgggactgc aacctataga gcgacaggtg
     3481 caggggttta tattgctatt ttgacaggag ctacagatgg tcgttccttc aacccaatct
     3541 tgcttgcagc gtcttacaac ggtgaaggta aattgacagg tggaagtgtt gattcaaaag
     3601 ctaaatatct ttatggtcaa acttctgtag ccaaatcaac tcttccaagt attgacaaga
     3661 cagtttcagg agtgactgca gatgctgata aaaattctgc ttcactaggt caagtattga
     3721 cttattcctt gaatgtgacg cttccaagct atccaactca ggctaaaaac aagacaattt
     3781 ttatttcaga tacaatgtct gaaggtttga catttgatta tgcaagcttg acaatttctt
     3841 ggaatggtca aacattgatt gctgacacta ctggtcaatt taaaactcaa ggggatattt
     3901 tgattgctaa ggcagttaaa gttggcaatg gctttaatct ggcatttgtc tatgataatt
     3961 tgaatgaaat tgcacctgta gtgacctata aaggtatcat caatgacaag gctgttgttg
     4021 gtggaacagg aaatgccaac aaggctgagt tcttctatgc gcgtaaccct aacagtggta
     4081 atacgtacga agatccaaat aataagccaa atcctcaaaa tgacaatact atcgtaaata
     4141 aagaagatag tgagacagtc tacacttacc aaatcgcctt taaaaaagta gattctatca
     4201 cgaaagcacc tcttgctggt gcagttttcg gaatttactc tgatgcggaa actaagcaat
     4261 tagtagatat tgtaactaca aatgcggatg gttatgctat ctcaacaaag gttggtaagg
     4321 gaacctacta tttgaaagag attactgcac caacaggcta ctcattaaac accaatgttt
     4381 atactgctga agcaagctgg acctcagcaa caacaacttc aagtgcaagc tcaactcgta
     4441 gtgaatacac ctcagttgtc ggtgaagcaa aggatagcac acaagttggt tggttgaaac
     4501 acaatgtctt ttataaattg gataataaac cagaaggtac cgatgtacaa gctgcctatt
     4561 tgaaagcaac aactactacg gcacaaaata caactgtgac aacaactaat ccaactgctg
     4621 gtgcgggtac agtatctatt gctgacgtac caaataccaa attaggagaa ttgccatcaa
     4681 caggtggaat gggaacatac ctattcacat ttatcggtgt cttagtgctt acagttggct
     4741 taggtttgta
//
LOCUS       DBNFEG010000159         4596 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.159, whole genome shotgun sequence.
ACCESSION   DBNFEG010000159 DBNFEG010000000
VERSION     DBNFEG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.159"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            29..259
                     /locus_tag="AC1PNG_001792"
     CDS             29..259
                     /locus_tag="AC1PNG_001792"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase subunit epsilon"
                     /protein_id="HIP6702275.1"
                     /translation="MIYKVFYQESKISPRREQTRALYLDIDAASELEGRIIARELVET
                     NRPEYNIEFIDLLSDNHLEYEKEHADFKITEF"
     gene            263..1942
                     /gene="rnjA"
                     /locus_tag="AC1PNG_001793"
     CDS             263..1942
                     /gene="rnjA"
                     /locus_tag="AC1PNG_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J1"
                     /protein_id="HIP6702276.1"
                     /translation="MSSVNLKPHEVGVFAIGGLGEIGKNTYGIEYKDEILIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVENLDRVKGLVITHGHEDHIGGIPFLLKQANVPIYAGPLA
                     LALIRGKLEEHGLLRDATLHEINHNTELTFKHLKVSFFRTTHSIPEPLGIVVDTPQGK
                     IVCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIM
                     KLIEGIHGRIIFASFASNIFRLQQAADAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKDTFIEPNEIKEYPASEIMILCTGSQGEPMAALSRIAHGTHRQVQLQPGDTVIFSSS
                     PIPGNTTGVNKLINILIEAGVDVIHGKINNIHTSGHGGQQEQKLMLRLIKPKYFMPVH
                     GEYRMQKIHASLAVDTGVPKDNIFIMENGDVLALTKDSAHLAGQFNAQDIYVDGNRIG
                     EIGAAVLKDRRDLSEDGVVLAVATVDFNSKMILAGPDILSRGFIYMRESGELIRESQR
                     VLFNAIRIAMRNKDANIQTVNGAIVNALRPFLYEKTEREPIIIPMILTPDR"
     gene            complement(2165..3511)
                     /gene="glnA"
                     /locus_tag="AC1PNG_001794"
     CDS             complement(2165..3511)
                     /gene="glnA"
                     /locus_tag="AC1PNG_001794"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIP6702277.1"
                     /translation="MVVTTADIKRDIKEKNVTFLRLMFTDIMGVMKNVEIPATEEQVE
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLNTWTIFPWGDENGRVAGLICDIYTTEG
                     EPFAGDPRGNLKKSLRRMEKEGFSSFNLGPEPEFFLFKMDEQGNPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTEMGFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDKDGNNAFFDPEDPRGMQLSETAY
                     HFLGGLIKHAYNYTAITNPTVNSYKRLVPGFEAPVYIAWAGKNRSPLVRVPASRGMGT
                     RLELRSVDPTANPYLAMAVLLEVGLDGIEHKIEAPAPVESNIYAMSDEERREAGITDL
                     PSTLHNALKALRKDDVVRSALGDHIYTNFLEAKKIEWASYATYVSQWEIDNYLDMY"
     gene            complement(3538..3909)
                     /locus_tag="AC1PNG_001795"
     CDS             complement(3538..3909)
                     /locus_tag="AC1PNG_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIP6702278.1"
                     /translation="MREKEFRRSLAIFPIGSVMKLTDLTARQIRYYEDHGLIKPVRNE
                     GNRRLYSLNDMDLLLEIKDFLDEGLNIAAIKKEYANRKAKEKVQAEQKTLTDEDVRRI
                     LHDEFSRQGRFSSPSSIFRSS"
     gene            complement(3989..4501)
                     /locus_tag="AC1PNG_001796"
     CDS             complement(3989..4501)
                     /locus_tag="AC1PNG_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HIP6702279.1"
                     /translation="MLKRYKFDTQKFKLGMRTLKSGLAVFLVILLFHLLKWDGIQIAA
                     LSAVFSLREDFGKSVHFGSSRVLSNSIGGFYALLYFILSDLFSHNFWVTLIFIPIFTM
                     LTIMTNVSFNNAPGVISGVATMLIIALSVPAGDSIFYALQRVFETFVGVFVSVIVNYD
                     IDKIKKRFLS"
BASE COUNT         1414 a         1094 c          840 g         1248 t
ORIGIN      
        1 agtcaaaaag aaaggaacta tactacacat gatttataaa gtattctatc aagaatcaaa
       61 aattagccca cgtcgcgagc aaacacgtgc tctttatcta gacatcgatg ctgcatcaga
      121 attggaaggt cgtattatcg cgcgtgaatt ggtggaaacc aaccgccctg aatacaacat
      181 cgaatttatc gacctcttat cagacaatca tcttgaatac gagaaagagc acgcagattt
      241 caaaattacg gagttttaat ccatgtcatc agtcaatcta aaacctcatg aagttggcgt
      301 tttcgccatc ggtggtttgg gagaaatcgg gaaaaatacc tacggtatcg agtataagga
      361 tgaaattctc atcgtcgatg ccggtatcaa gttcccagaa gatgacctat tgggtatcga
      421 ctacgttatc ccagactact cctacatcgt tgaaaaccta gaccgtgtta aaggtctggt
      481 tattacccac gggcacgaag accacatcgg aggcattccc ttcttactca agcaggccaa
      541 tgtcccaatc tatgctggac ctttagccct tgcccttatt cgaggcaaat tggaagaaca
      601 cggactgctc cgtgatgcga ctttgcatga aatcaatcat aatactgagt tgaccttcaa
      661 acacctcaag gtcagcttct tccgcaccac ccactccatt ccagaaccac ttggtatcgt
      721 cgttgacacc ccgcaaggaa aaatcgtctg caccggtgac ttcaagttcg acttcactcc
      781 agttggcgag ccagcagacc ttcaccgcat ggctgccctc ggtgaagaag gcgttctctg
      841 cctcctctcc gactcgacaa atgctgaggt cccaaccttt accaactcgg aaaaagtggt
      901 cggtcagtcc attatgaagc tgattgaagg cattcacgga cgcatcattt ttgcttcctt
      961 tgcctccaac atcttccgtc tccaacaggc ggctgatgca gcggttaaga ccggacgcaa
     1021 gatcgctgtc tttggacgtt ccatggaaaa agccattgtt aacggtatcg agctcggcta
     1081 catcaaggtt ccaaaagaca cctttatcga gccaaacgag atcaaagagt atcctgccag
     1141 cgagatcatg attctctgta cagggagcca aggggagcct atggcagcac tctctcgcat
     1201 cgcccacggt acccaccgtc aggtgcaact ccagccaggc gacacggtta tcttctcgtc
     1261 cagtcctatt ccgggaaata ccacaggtgt caacaagctc atcaatatct taattgaagc
     1321 cggtgtcgat gttattcacg gcaagatcaa caacatccat acctctggac acggtggtca
     1381 acaagagcaa aaactcatgc tccgcctaat caagccaaaa tacttcatgc ccgtccatgg
     1441 tgaataccgc atgcaaaaaa ttcatgccag cctagctgtt gatacaggcg ttccaaaaga
     1501 taatatcttc atcatggaaa acggagatgt ccttgctctg acaaaagatt ctgcacatct
     1561 cgcaggtcaa ttcaacgccc aagacatcta tgttgatgga aaccgcatcg gtgaaatcgg
     1621 cgcagctgtt ctaaaagacc gtcgcgatct ttcagaagat ggtgttgtcc ttgcagtagc
     1681 cactgtcgat tttaattcta agatgatatt agcaggacca gacatcctca gccgtggctt
     1741 catctatatg agagaatctg gcgagctcat tcgcgaaagc caacgcgtcc ttttcaacgc
     1801 tatccgcatc gccatgcgca ataaagatgc caatatccaa acagtcaacg gcgccattgt
     1861 taacgcccta cgaccattcc tatacgaaaa aacagagcgt gaaccaatca ttatcccaat
     1921 gattctgacg cctgaccgat aacacactaa aatcccagag gtgagacctc tgggatttat
     1981 tttatattaa caagataacc cagcttgtaa aaagacagat ttcaagctca tgtccatccc
     2041 ctcgccctgc tatttattag ctcagactga aacagtctct tcccaggcta tttcactcca
     2101 ctggaccatt acacgctttc caatttctcg gctcgtgaac aaaggacccg tcggggtcct
     2161 ttgtttaata catatccaaa tagttgtcaa tttcccattg agaaacgtag gttgcatagc
     2221 ttgcccattc gattttctta gcttccaaga agttagtata gatatgatca cccaatgccg
     2281 aacgaacaac atcatctttt ctcaaggctt taagcgcatt gtgaagagtt gatggaagat
     2341 ctgtaatacc agcttcgcga cgttcttcat ctgacattgc ataaatattt gattctactg
     2401 gagcaggagc ctcaatcttg tgttcgatac catctaatcc aacttccaaa agaactgcca
     2461 tagccaaata tgggttagcc gttggatcta cagaacgcaa ttctaagcga gttcccattc
     2521 cacgtgatgc aggtacacga accaatggag aacggttttt gcctgcccaa gcaatataaa
     2581 caggagcttc aaaaccaggt accaaacgtt tgtatgagtt tactgtaggg ttggtaattg
     2641 cagtgtagtt ataagcatgt ttaatcaaac caccgaggaa gtgataagct gtctctgaca
     2701 attgcatgcc acgaggatct tctggatcaa agaaggcatt attaccgtct ttatcaaaga
     2761 gtgacatgtt acagtgcata cctgaaccag caattccaaa tttaggttta gccataaatg
     2821 tagcgtacaa gccatgtttt cgtgcaatcg tcttaacaac caacttaaag atttggatat
     2881 tatcacacgc cttcaaaaca tccgcatatt taaagtcgat ttcatgctga ccaaccgcta
     2941 cttcgtggtg gctcgcttca acttcaaatc ccatttcagt caaaacattt acaatttcac
     3001 gacgagtatt gtccgcaaga tctgtaggcg ccaagtcaaa gtaaccaccc ttgtcattca
     3061 cttcaagagt tggattcccc tgctcatcca ttttgaacaa gaagaattct ggttctggac
     3121 caaggttaaa ggatgagaat ccttcttttt ccatccgacg aagagatttt ttcaagtttc
     3181 cacgaggatc tcccgcaaac ggttcacctt cagtcgtata aatatcacaa atcaagcctg
     3241 ccacacggcc attttcatct ccccaaggaa aaatagtcca tgtgtttaaa tctggataaa
     3301 ggtacatatc agattcatta atacgtacaa aaccttcaat agacgaacca tcaaacataa
     3361 ctttatttga taaaactttt tcaacctgct cttctgtcgc aggaatctct acgtttttca
     3421 tcactcccat aatatcagtg aacatgagac gtagaaatgt aacatttttc tctttaatat
     3481 cacgtttaat atccgctgtt gtgacaacca tattttttct cctcttaatt aaacctatta
     3541 cgacgaccga aagatcgagc ttggacttga aaatcgccct tgtctgctaa attcatcatg
     3601 taggatacga cgcacatcct catcagttaa agttttctgt tctgcttgaa ccttttcctt
     3661 tgctttacga ttggcgtatt cttttttaat cgctgcaatg tttaggccct catccaaaaa
     3721 atctttaatt tcaagtagta agtccatatc atttagagaa tacaagcggc gattaccttc
     3781 gttccgaact ggttttatca aaccgtgatc ttcatagtaa cgaatctgac gcgctgttaa
     3841 atcagtcaat ttcattacac taccaattgg aaaaatagct aaggaacgtc gaaactcttt
     3901 ttctctcata atgaactcct ttctgcacac tattataggg cagaaaaaac gaacagtcaa
     3961 gccctctgtg tcacatttta tgacactgtt aggaaagaaa gcgctttttt attttatcga
     4021 tatcgtaatt tacaataaca gatacaaaaa ctccaacgaa tgtttcaaaa actctttgga
     4081 gggcgtagaa aatagaatct cctgcaggaa ctgagagagc aatgatcaac attgtagcaa
     4141 ccccgctaat aacacctgga gcattattaa acgaaacatt cgtcataata gttagcatcg
     4201 taaaaatagg gatgaaaatt aaggtcaccc aaaaattatg agaaaataga tcacttagaa
     4261 taaaataaag caaggcataa aagccaccta tggaattact aagtacacgg gaagagccaa
     4321 aatgcacact tttcccaaaa tcttcacgaa gactgaaaac tgctgagaga gccgcaatct
     4381 gaataccatc ccattttaaa agatgaaaca ataaaatgac taaaaataca gctagacctg
     4441 attttaaagt tctcataccc aatttaaact tctgggtatc aaatttatat ctctttaaca
     4501 tacttctatt ctaacatgat ttgttaaaaa atgattctaa atgcacataa tgttatgaat
     4561 cattcggaca ttttagtgta aaaataatac cagata
//
LOCUS       DBNFEG010000160         4594 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.160, whole genome shotgun sequence.
ACCESSION   DBNFEG010000160 DBNFEG010000000
VERSION     DBNFEG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.160"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(392..1030)
                     /locus_tag="AC1PNG_001797"
     CDS             complement(392..1030)
                     /locus_tag="AC1PNG_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIP6702280.1"
                     /translation="MRWGIVVEQLTVLYQNLKRRYGEFHWWNDENPIKDLVSTILIQQ
                     TTEANAKRALEQLEGRLTIHSLLEMPVEDLQECIRPAGFFKQKSLYIRSVVEWANQFD
                     GDFSRLDRVETAVLRKELLSLKGVGNETADVILLYLCRRSIFVADQYALRLFNRLGLS
                     QSQDYLSLRQEFTEQIKDWSVKDAQELHALIDEHGKQFRLTKGQLDESWLMI"
     gene            complement(1078..1233)
                     /locus_tag="AC1PNG_001798"
     CDS             complement(1078..1233)
                     /locus_tag="AC1PNG_001798"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702281.1"
                     /translation="MLVKGISQGQAPEIFLKEAAAFATANCLNYFPEVEAEQFEAIVD
                     TIVLEEL"
     gene            complement(1288..1998)
                     /locus_tag="AC1PNG_001799"
     CDS             complement(1288..1998)
                     /locus_tag="AC1PNG_001799"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HIP6702282.1"
                     /translation="MIHLICPNPALDRTLLVEKIEKNIPLRPTEVRDYPGGKSFNVAY
                     ALRENGVTDYTIHTILGGQIGRYIQELNADGGNALRVVENSQNTRTCNIYVETSTGDV
                     VLFYEKGFELTEDLLNQFTQQIENSLQAGDILVFSGSLMKGVSDDYIQRFIEKYPEVL
                     TIVDTSGPALRAAYQARPALIKINNEELKDIYPELDEESPEAILGILKEVTPHENIII
                     TMGDKGSLAKIGQRFFRI"
     gene            complement(2100..2726)
                     /locus_tag="AC1PNG_001800"
     CDS             complement(2100..2726)
                     /locus_tag="AC1PNG_001800"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIP6702283.1"
                     /translation="MKIAIIAANGQAGQTIAKEAVERGHQVTAIVRSENKSVAQEVIQ
                     KDAFALTKEDLAGFDVVVNAFGAWTPETLPDHARLAEHLATILAGSPTRLLVVGGAGS
                     LYLDESQTAMLKDTPDFPAEYLPIAEAMGTGLDLYRQATAVNWTYISPAADFDAEGQK
                     SGAYTLAGEVFQVNAQGESYISYADYAVALVDIAEKGGYQQERISVLA"
     gene            complement(2965..3384)
                     /locus_tag="AC1PNG_001801"
     CDS             complement(2965..3384)
                     /locus_tag="AC1PNG_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HIP6702284.1"
                     /translation="MQISSRFTTASHILVLLALEGEKEKQTSTSIAGSVGVNPVIIRN
                     ILSQLKEAGLVEVARGVGGARLAQGPDQITLLHVYQAVELFGEKGQLFAFHEQPNPSC
                     QVGRNIHPLLDSRLENAQSALENELAKTRLADLLAEL"
     gene            complement(3427..4494)
                     /gene="dinB"
                     /locus_tag="AC1PNG_001802"
     CDS             complement(3427..4494)
                     /gene="dinB"
                     /locus_tag="AC1PNG_001802"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIP6702285.1"
                     /translation="MLIFPLINDLSRKIIHVDMDAFFAAIEVRDNPALKGKPVIIGAD
                     PRLSGGRGVVSTCNYEARAFGIHSAMSSKEAYECCPQAIFISGNYEKYQEVGRQVREI
                     FHRYTDLVEPMSIDEAYLDVTENKLGISSAVKIAKLIQYDIWNELHLTASAGVSYNKF
                     LAKIASDMEKPYGLTLILPEDAVGILASLPVEKFHGVGKKTVERLHEMGVYTGQDLLD
                     VPEMVLIDRFGRFGFDLYRKARGISNSPVKVDRVRKSIGKERTYRKLLYREEDVLKEL
                     ISLCQRVAASLKRNGKKGRTIVLKVRYGDFSTLTKRHSLEVYTDQTETIEKEVRQLIE
                     EIGKIEKGIRLLGVTVTNFQT"
BASE COUNT         1323 a         1097 c          824 g         1350 t
ORIGIN      
        1 aaataattta gatattaaat tcttatcttt taatatattt cgaggtgcaa ttggagcagc
       61 atccttagca gtctataatc attttatctt ggaaaataac tccaattaat ataaaaaaag
      121 agcaaccaac tgaaccgctg gttttactct tttatagtcg tttcgtttgc ttttagtaca
      181 aggcaacgat atgcaggcta cttcaactgt ccagtggagt gtttaaactc ggaaataagg
      241 aaatgagact tcctcgccct tcagtaataa aaaatacact aattgatact caatgaaaat
      301 caaaatcaag ctaggcgacg caaatgcaga tagaactgag gttcatcaaa tcatgtcaac
      361 aacgtctgat tttgattttc gaagagtatg actatatcat caaccatgat tcatcaagct
      421 gcccctttgt taacctgaac tgcttgccat gttcatcaat cagtgcatga agctcctgag
      481 cgtcttttac cgaccaatct tttatctgct ctgtaaattc ctgtctcaaa ctcaaataat
      541 cctgagactg gctaagtcct aaacgattaa atagccgaag agcatattga tccgccacaa
      601 aaatacttct tcgacacaaa tagagcaaaa taacatccgc agtttcattg ccaaccccct
      661 tcaagctcag cagctctttt cgaagtactg ctgtctcaac acgatctagg cgagaaaaat
      721 ctccatcgaa ctgattggcc cactcaacca ctgaccgaat gtagagtgat ttttgcttga
      781 agaatcctgc tgggcgaatg cattcttgta aatcctccac aggcatctct aaaagcgaat
      841 gaattgtcaa ccgtccctcc aactgttcta aagcacgctt ggcatttgct tccgttgttt
      901 gctgaataag aatcgtagat accaaatcct ttataggatt ttcatcattc caccagtgaa
      961 attcaccata tcgccgtttt aaattttggt aaagaaccgt caactgttcc actactatcc
     1021 cccatctcac cttgtttctc cttgccatta tagcacgatg agtaaaggga tacaatacta
     1081 cagttcctct agtactattg tatctacaat cgcttcaaac tgctctgctt caacctctgg
     1141 aaaataattc aaacaattgg ctgttgcaaa ggctgccgct tctttcaaaa agatttctgg
     1201 ggcttgacct tgactgattc ctttgactag caagcccagg tagaaatccc ctgcggcaat
     1261 aggattacgc gtttctttct ttggagacta aatacggaag aagcgctgac caatcttagc
     1321 tagactcccc ttgtctccca tggtaatgat gatattctca tgaggagtca cttctttcag
     1381 gatacctaaa atagcttcag gactttcttc atccagctct ggataaatat ctttcagctc
     1441 ttcattattg attttgataa gggccggtct agcttgataa gccgcacgta aagctggtcc
     1501 actggtgtcc actatggtca gaacttctgg gtatttttca ataaaccgct gaatataatc
     1561 atccgacacc cccttcatca gactacctga aaagactagg atgtcgccag cttgcaggct
     1621 attttcaatc tgctgagtga attggttgag caaatcctct gttagttcaa aacctttttc
     1681 ataaaatagt actacatcgc ctgtactggt ttcaacatag atattacatg tccgagtatt
     1741 ctggctattt tcaaccacgc gcagggcatt tccaccatca gcattaagct cctgaatata
     1801 gcgaccaatc tgcccaccta aaatggtatg gatagtgtag tctgtcactc cattttctcg
     1861 aagtgcatag gctacgttga aactttttcc accaggataa tccctcactt ccgtcggtct
     1921 caggggaata ttcttttcaa tcttttccac aagaagcgtg cggtctaggg ctggattggg
     1981 acaaatcaag tgaatcatgg ggttctcctt ttctttttat ttatattatt atactatatg
     2041 caagtaaaaa ataaaaagat aattcctcga tggagctatc ttttagacca tttaattttt
     2101 tatgcaagta cggaaattcg ttcctgctgg tagccacctt tttccgcaat gtcaaccaaa
     2161 gccacagcat agtcggcata actaatatag ctttcacctt gggcattgac ttggaaaacc
     2221 tcgccagcca aagtgtaggc acctgatttt tgaccttctg catcaaaatc tgctgctggg
     2281 ctgatatagg tccagttgac cgctgtcgcc tgacgataga ggtctagacc agtacccata
     2341 gcctcagcga ttggcaggta ttccgcaggg aaatctggtg tatctttcag catagctgtc
     2401 tggctctcgt ctaaataaag actgcctgca ccaccaacta ctaaaagacg agttggacta
     2461 ccagcaagta tggtcgctag atgctcagca agacgagcgt gatccggcaa ggtttctggt
     2521 gtccaagcac caaaagcatt aactactaca tcaaagcctg ccaaatcttc tttggtaaga
     2581 gcaaaagcat ccttctggat tacttcttgg gctacgctct tgttttcaga acggacaata
     2641 gccgtcacct gatgaccacg ttctactgct tcctttgcaa tagtttgacc tgcttgacca
     2701 ttcgctgcaa taatagcaat tttcatattg atacctcact tgtttcgttg taacaacttc
     2761 gtttacaacg taagtataaa ttattttggt tgtaacgtca attgttacag cttgaaaatt
     2821 aaaaaaatca tcacaaggat gattcactaa ctagtctttt agtgctaggg caacactcta
     2881 cagactactt caacagtcca gtggagtgtt tgaagtagga aatagggaaa caaagtttct
     2941 ccttacgttg acctaataaa acgactatag ttccgccaag agatcagcaa gccttgtctt
     3001 agcaagttca ttttccaaag ccgactgagc attttccaaa cggctatcta gcagaggatg
     3061 aatattgcga cctacttggc aggaaggatt cggttgctca tgaaaagcga ataattgacc
     3121 tttttctcca aataactcca cagcctgata gacatgcaaa agtgtaatct ggtctggacc
     3181 ctgtgccaag cgagcacccc caacaccacg cgctacttct accagaccag cctccttgag
     3241 ttgggacaaa atatttcgga taatgacagg attaaccccg acactgcctg caatgctagt
     3301 actggtctgt ttttcttttt ctccttccag agccaagaga accaagatat ggctggcagt
     3361 ggtaaatcta cttgaaattt gcatctcttc tccttttttt cagtctatca tagactgaaa
     3421 cagaactcaa gtttgaaaat tagtcacggt cacacccagc aaacgaatcc ctttttcaat
     3481 cttcccaatt tcttcaatta gttgacgaac ctctttctca atcgtttccg tttggtccgt
     3541 atacacctct agactatgac gtttcgtcaa ggtagaaaaa tcaccataac gtacctttaa
     3601 aacaattgtt cgcccctttt ttccatttcg cttcaaactg gctgcgaccc gttggcagag
     3661 actgattagc tctttcagga catcctcctc ccgataaaga agtttgcgat aggttctttc
     3721 ctttccaatt gacttacgaa cacggtccac tttaacgggc gaatttgaaa tccccctcgc
     3781 tttccgataa aggtcgaagc caaaacggcc aaatctatct atcaaaacca tttctggtac
     3841 atctagtaaa tcttgccccg tgtagacccc catctcatgc aggcgttcca ccgtcttctt
     3901 ccccacaccg tgaaattttt caacaggcag ggaggcgaga ataccaacag catcttcagg
     3961 tagaatcaag gtcaaaccgt acggtttttc catatcagat gcaatttttg ccaaaaattt
     4021 attataggaa acgcctgcgg aagctgtcag atgcaattca ttccaaatat cgtattggat
     4081 aagcttggca atcttaaccg cagagctgat gcccaactta ttttctgtta catccaagta
     4141 agcctcatca atagacatgg gctccaccaa atcagtatag cgatgaaaaa tttctcgaac
     4201 ctgtcgccca acttcctgat atttttcata attgccagaa atgaaaatcg cctgaggaca
     4261 acactcatag gcctcttttg aggacatagc cgagtgaatc ccaaaggcac gcgcctcata
     4321 attacaggta gataccactc ctctaccgcc agatagtctt gggtcagccc caatgataac
     4381 aggtttgccc ttcaaagcgg gattatccct gacctcaatg gcagcaaaaa aagcatccat
     4441 atctacatga ataatttttc tagataaatc attgattaag ggaaaaatta acatgactgc
     4501 ctcttcctgt ttacaagttt gactcgctat attataccgc atttcttagg aagtttcagc
     4561 ctttagattc gttgaaccag ccatcttatc aata
//
LOCUS       DBNFEG010000161         4490 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.161, whole genome shotgun sequence.
ACCESSION   DBNFEG010000161 DBNFEG010000000
VERSION     DBNFEG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.161"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            13..1737
                     /gene="ezrA"
                     /locus_tag="AC1PNG_001803"
     CDS             13..1737
                     /gene="ezrA"
                     /locus_tag="AC1PNG_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HIP6702286.1"
                     /translation="MPTGTIILIVSIVIILIIAYVACLIVRKRNDNLLVALEERKEEL
                     FNLPVNEEVEAVKALHLIGQSQVSFREWNQKWVDLSLNSFADIENHIFEAEGYNNAFR
                     FVSAKNAIDSIESQIDLIEEDIASIRHGLMELKEQEEKNSGRVKHALNLFDSLQEAVR
                     ENPDSYGETLPELEKQLKNIEVEFSEFVMLNSSGDPIEASEILDKTEEHMIALNQIMD
                     RIPSLIERVTKDFPEQLEDLESGYRKLVEQNYLFTEANIESQFQNIRVSIRENTALIV
                     SFDLDAAEAENEGIQAKIDHLYKVFNREIEANKEAVKISKNLPKFLEHVVQNTQLLDE
                     ESQRLNATYLLADSKLSRINQLKARLESIEIVVTESVEDIENPQVAYSILEERLDHSL
                     ASLKEIEEEQLVLADYLKSQELSENTARKKATLYINKLHTLKRYMKKRNLPGIPAEFL
                     TNFFRTSDHVEALIAELDYKRINIEVVNRLLENATYDMNQLEELAYLIVQNATLTEQL
                     LQYSNRYRSFDESVQKAFNRSLSIFEKDFDYQAAFEEISFALETVEPGVTERFVRSYE
                     KTREAIRY"
     gene            1789..2424
                     /gene="serB"
                     /locus_tag="AC1PNG_001804"
     CDS             1789..2424
                     /gene="serB"
                     /locus_tag="AC1PNG_001804"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HIP6702287.1"
                     /translation="MTTGLLVMDVDSTLILEEGIDLLGEEAGLGAQVAAITERAMRGE
                     LDFEEALRERVALLKGLPVSVFDRIIKKIHFTPGAAELVSELKMRGYKVAVVSGGFHE
                     TVDRLAAQLELDYVRANRLEVVDGVLTGQVLGEIVTKDTKKACLEEWAAENGLSLSQT
                     IAMGDGANDLPMIQRAGIGVAFCAKPIVQEQAPYQINEKNLYKLIEILDSK"
     gene            complement(2455..3105)
                     /locus_tag="AC1PNG_001805"
     CDS             complement(2455..3105)
                     /locus_tag="AC1PNG_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIP6702288.1"
                     /translation="MYQTILFDLDGTLTDSGQGILNSVAYALEKMGIEEPDTANLNRF
                     IGPPLYESFSRFYQLNPEDTQSAVDTFRVYFKEKGMFENQLYPGIIPLLEELRTAGKT
                     LVIATSKPEIFAKQILEHFGIAHYFDIIAGASLDSSRISKADVIGYAINQLEAFPKHA
                     VMIGDREHDIEGARMHQLPAIGVLYGYGNKQEFEKAGATMIVETVQDLKRVLLTTE"
     gene            3213..4340
                     /locus_tag="AC1PNG_001806"
     CDS             3213..4340
                     /locus_tag="AC1PNG_001806"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HIP6702289.1"
                     /translation="MHILIAPDSFKESLSATKVAEAIKKGFSKVYPQAGYDLIPLGDG
                     GEGTVESLMQALSLDGTQTWVTGPFGDKIKVSYAVKDDLAVFEMAEIVGLASIPHERR
                     SPLFIKTQGVGELIVELVQNGVKRIFIGVGGSASHDGGIGMAAGLGVKFYNREGKEVE
                     AIGAHIGEIVSFSTEDMLVDLSQVRIDLVTDVENPLCGPAGATFVFAGQKGLAPAEFE
                     LVDKKMESFYKLVNPMLLDLAGAGAGGGMAAGLVQFAGARIQKGIDFVLDQLDFAHRV
                     TKADLVVVGEGRMDAQSLSGKTPIGVARRTPTGIPIIAICGSLKDDLPEFPFENICAA
                     FPIIASVESLEDTLQKAEKNLVRTAEQVARILQLGGQQRWN"
BASE COUNT         1375 a          758 c         1054 g         1303 t
ORIGIN      
        1 aaggagattt acatgcctac aggaacaatc atcttaatcg tttcgattgt tattatttta
       61 atcattgcct atgtagcttg cctgattgtt cgtaaacgaa atgacaacct tttggttgca
      121 ttggaagaac gtaaggaaga gctatttaac cttccagtta atgaagaagt cgaagctgtc
      181 aaagcacttc atttgattgg tcaaagtcag gtgtcattcc gtgaatggaa tcaaaaatgg
      241 gttgatttgt cgctcaattc atttgccgac attgaaaatc atatctttga ggcagaaggc
      301 tataacaatg cctttcgctt tgtgagtgca aagaacgcta tcgacagtat cgaaagccaa
      361 attgatttaa ttgaagaaga catcgcctct atccgtcatg gtttgatgga gttgaaggag
      421 caagaagaga aaaactctgg tcgcgtaaaa catgcgttaa acttgttcga tagtttacag
      481 gaagctgttc gtgaaaatcc agatagctac ggtgagacac taccagaact tgagaaacaa
      541 ttgaaaaata ttgaagttga gttttctgaa tttgtgatgc tcaattcctc tggtgacccg
      601 attgaagctt ctgaaatcct tgataagact gaagagcata tgattgccct caatcaaatc
      661 atggatcgca ttccaagttt gattgaacga gttacaaaag atttcccaga gcaattggaa
      721 gatttggagt cgggttatcg taaactggtt gagcagaatt atttgttcac agaagctaac
      781 atcgagtcac agttccaaaa tattcgcgtt tcaattcgtg aaaatactgc tttgattgtt
      841 tcctttgatt tggatgccgc agaagctgaa aatgagggaa ttcaagcaaa aattgatcat
      901 ttgtataaag tcttcaatcg tgagatcgaa gcgaataagg aagctgttaa gattagtaaa
      961 aacttgccaa aattcttgga gcatgttgtc caaaatacac agctcttgga tgaggaaagc
     1021 caacgcctaa atgcaactta cttgctggca gatagcaaac tttcacggat caatcagctt
     1081 aaagctcgtt tggaatcgat tgaaattgtt gttacagaaa gtgtggaaga cattgaaaat
     1141 ccacaagttg catattctat tttggaagag cgtttggacc attcattggc aagcttgaaa
     1201 gaaatcgaag aggagcagct tgttttagct gactatctta aatcacaaga attgtcagag
     1261 aacacagcgc gtaagaaagc tacactttat atcaacaaat tgcataccct taagcgttat
     1321 atgaaaaaac gcaaccttcc aggaattcca gctgaatttt taacaaactt ctttagaacg
     1381 agcgatcatg tggaagcctt gattgccgag ttagactata aacgaatcaa tatcgaagtt
     1441 gttaatcgtt tgttggaaaa tgcaacgtat gatatgaatc aattggaaga gttggcgtat
     1501 ttgattgtgc aaaatgcgac tttaacagag cagttgctac aatactctaa ccgttatcgt
     1561 tcattcgatg aaagtgtgca aaaagcattt aaccgttcat tatcaatttt tgaaaaggat
     1621 tttgattacc aagcagcatt tgaagaaatt tcatttgctc tggaaacagt tgaacctggt
     1681 gtaacagagc gttttgtacg ttcatacgaa aaaactcgcg aagccattcg ctactagata
     1741 aaaagccatt tggcttttta tttttatact ttgaaaggaa tattggagat gacaactgga
     1801 ttattagtta tggatgttga ttcaacatta attttggaag aaggaataga tttacttgga
     1861 gaagaggccg gtttaggagc gcaagttgca gcaattaccg aacgtgctat gcgtggggaa
     1921 ttggattttg aagaagcctt acgtgaacgt gtggcgttgt taaagggact gcctgtttct
     1981 gtgtttgatc gaattatcaa aaaaatccac tttacgcctg gagcagcaga attggtctca
     2041 gaattaaaaa tgcgaggcta taaggtagca gtagtttcag gcgggtttca tgaaacagtc
     2101 gatcgactag ctgcacagtt agagttagac tatgttcggg ctaatcgatt agaagtagtg
     2161 gatggagtat tgacgggaca agtattgggt gaaattgtaa cgaaagatac taaaaaagca
     2221 tgtttagaag aatgggcggc agaaaatggg ctgagtctat ctcaaacgat tgccatgggg
     2281 gatggagcaa atgatctacc aatgattcaa cgagcaggaa ttggagtagc tttctgtgct
     2341 aaaccgattg tccaagaaca ggcgccctac caaataaatg aaaaaaatct ctataaacta
     2401 atagagattt tagatagtaa ataacgagaa gtcagctgag tgctgacttt tcttctattc
     2461 tgttgtcagt aaaacccttt tcaaatcttg gacggtttcg actatcatgg tagccccagc
     2521 cttttcaaat tcctgcttgt tcccatagcc gtaaagaaca ccgatggcgg gaagttggtg
     2581 catgcgtgct ccttcaatgt catgttccct atctccaatc atcacagcat gttttggaaa
     2641 tgcttctaat tgatttatag catagccgat gacgtccgcc ttactgatgc gactactatc
     2701 cagactagca ccggcaatga tatcaaagta gtgagcgata ccaaaatgtt ctagaatctg
     2761 tttggcaaat atttctggct ttgaggtagc aattactagt gtttttccag cagttctcaa
     2821 ttcttcaagc agagggatga ttccaggata gagttgattt tcaaacattc ctttttcttt
     2881 aaaatagaca cggaaagtat caactgcact ttgtgtatct tcagggttga gttggtagaa
     2941 tcgtgaaaaa gattcataga gtggcgggcc gatgaatcgg tttaaattgg ctgtatctgg
     3001 ttcttcgatt cccatttttt ctagtgcgta ggcgacggag tttaagattc cttgtccaga
     3061 atcagtaagt gttccatcta agtcaaataa tatagtttga tacatactta tctcctttct
     3121 ttcagtataa cacagacgat gaaaaacaga aaagatttat tgaaagctga ataggagcgt
     3181 gatatactaa taatataaag gaggctagta ccatgcatat tctcattgct ccggattcat
     3241 ttaaagagtc tctttcagct acaaaggttg ctgaagcgat taaaaaagga ttttctaagg
     3301 tttacccaca agcagggtat gacttaattc ccctaggaga tggtggcgaa gggacagttg
     3361 agagtctaat gcaggcctta tccttggatg ggacccagac ttgggtgaca ggtccatttg
     3421 gagataaaat taaggtttcc tatgcggtta aagatgatct tgcggttttt gaaatggctg
     3481 agattgtagg gctggcaagc atcccgcatg aaagaaggag tcctctcttt attaagacac
     3541 aaggggttgg ggagctgatt gtggagctcg ttcaaaatgg tgtcaaacgc atatttatag
     3601 gagtaggtgg ctctgccagt catgatggtg gtatcggaat ggcggcaggt ttaggagtga
     3661 aattttataa tcgagaggga aaagaagtag aggcaattgg agctcatatc ggggagattg
     3721 tcagcttttc cacagaggat atgttggtag atctatctca ggtcaggatt gatttggtaa
     3781 cggatgtgga gaatccgctt tgcggaccag cgggtgccac atttgttttt gcaggtcaga
     3841 aaggtcttgc ccctgctgaa tttgaactag tagacaagaa aatggagtcc ttttataaat
     3901 tagttaatcc aatgctactc gatttggcag gagcgggtgc tggaggaggt atggcagcag
     3961 gtttagtcca atttgctggt gctcgcattc aaaaaggcat cgactttgtc ttggatcagt
     4021 tggactttgc tcatcgcgtc accaaagcgg atctggttgt ggtcggagaa gggcggatgg
     4081 atgcccaaag cttatcaggg aagactccga taggagtagc tagacggacg ccgacgggga
     4141 ttcccatcat tgcaatttgt ggtagcctca aagacgatct tcccgagttt ccttttgaaa
     4201 atatctgtgc cgcttttccg ataattgcaa gcgttgaaag tttggaggac acgcttcaaa
     4261 aagcagaaaa aaacctggtc cgcacggcag agcaggtggc aagaattctt cagttaggag
     4321 gtcaacaaag atggaattag tgaaagatgt ggctattatc ttatttattt ttatttgtct
     4381 tgcaaagcta gtatccgtta tgagactttc cgaaaaagaa agaggaatgg aaatgaatgt
     4441 aataaaggca aatggagcaa tgttcttttt caatgaaaaa aatgaaagag
//
LOCUS       DBNFEG010000162         4467 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.162, whole genome shotgun sequence.
ACCESSION   DBNFEG010000162 DBNFEG010000000
VERSION     DBNFEG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.162"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            320..607
                     /locus_tag="AC1PNG_001807"
                     /pseudo
     CDS             320..607
                     /locus_tag="AC1PNG_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1131..1769)
                     /locus_tag="AC1PNG_001808"
     CDS             complement(1131..1769)
                     /locus_tag="AC1PNG_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIP6702290.1"
                     /translation="MIIHKSVNPVAFQNTYYLENDTHLIIIDPGSDWTKIQVTIEKIG
                     KPISAILLTHTHYDHIMSLDILRETYNFPPVYVAESEASWLYTPEMNLSGLPRHDDME
                     NVVCRPAEKIFQYQEDYQFDGFHFKVVPTPGHSIGGVSFIFPEDECVITGDALFRETV
                     GRTDLPTSNFDDLITGIREHIFTLPNHYTVHPGHGQNTSVGHEKNFNPFFRM"
     gene            1877..4345
                     /locus_tag="AC1PNG_001809"
     CDS             1877..4345
                     /locus_tag="AC1PNG_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HIP6702291.1"
                     /translation="MNSMKDTRTSNRYAVVDLEATGTGTDAKIIQIGIVLVENGEIID
                     SYATDINPYELLDDHIKNLTGITDQQLSQAPDFGQVASTIYDMIGDAIFVAHNVKFDA
                     NLLAEALFFEGYELLTPRVDTVELSQLFFPTFEKYSLGNLAEHLELGLDQAHTAISDA
                     MATARLLIKIQEKIKSLPRSIVEKILDLADNLLFESRLVIDEMVPVLSENLSYDLESI
                     HDLVLKKPEEIKSAYRLSEDFSTNIALLGLHERKKQTAFAQVVEKRLVDEEKVHFIQA
                     QAGLGKTYGYLLPLLAKSDQPLLVTVPTKLLQEQIMEKEGSKLKEIFRIPIVSLKSPK
                     HFIKLDNYWKTLQRQDDNRLVNQFKMQVLVWLCETTTGDLDELKQKQRYQAYFDEIAH
                     DGKLEPESLFWGWDFWQRLNHQALYSRLLITNHAYFLNHLKDQDPLMDKRLVAIDEAQ
                     KFLLAAENLATASQDLTSMLQVLQSKKDRATTILDQRLYESCQFELNHFLSRFRQHGQ
                     REVQKAELHQLGQNLEELGDVDLNDLHQLIDYYDTFWLEEKNLEEKRLAYLRGSKDSL
                     LNATNYLPDTKIFCISATLTISKKVSLADLLGFEQVTLDLIPTQTVTNQQLLFPTHLP
                     DILAWTKEEHAAYLATILGEIAELGRPILVLFTSISMLLQVSDLLEDNNIPHLAQHKH
                     GQEMTLKRKFERGECQILLGTGVFWEGVDFASQNQLIQVITRLPFDNPKDRFVQKINH
                     HLREEGKNPFYDYSLPMMMIKLKQAIGRTNRHDKQDSLVLVLDPRVHTKRYGQQILSF
                     FEKDYSMLSVDAKEIEGAIQDFFE"
BASE COUNT         1358 a          862 c          954 g         1293 t
ORIGIN      
        1 cttcgttttt cccactccct aggatctttt tattatatga cttttagtcc atcacaatcc
       61 gtaaagtatg tgacaaatga accgaggatg ggcaaaaaat ggtcctataa tttctgtagc
      121 gggtaaaacc actatggagc cttttaaatg taaaagccat atgacctata atgaaaagtg
      181 acaaaactta cattagaaag aggtcatata gaataaatca agagttaggg gagtgactct
      241 tgattcctga gcctagaaat agaaaagcga agatcttatc acaaattttc ttggaataga
      301 agacaaaaat atcgctatca tgactgaata tgatgtggaa actcacttag attgtgtgtc
      361 tccaaaatgt cttgcctgaa agggacaaat agtgaaatac aacttccaaa gagcatctaa
      421 aatcctttgc ttagaagcag ctgataatcc tctctcctta tccatcttag aaagcgtcgg
      481 ttcaagtgta aaggatgcgg gaaaatggtt gtcgctgaaa ttcacttatt aaaaaaatga
      541 ccaaatctct gttgctatta accagaaaaa accccaatta ctactcgttg agaatcaagc
      601 aatgagtaag caatgttcac ctgatactat tgtcatcaag gatagctttt ctatcctatt
      661 cggaactata aagtaagagt ttttgaaggg ggtagatatt ggttgcatgt attgaaaaac
      721 ttgtggttcc atggttttta aagttactct atctggctat attttgaact atgttattac
      781 accagtttct gattatgtag tgaattgctt gttggaaggc tatcagtaat ctgtgatttt
      841 agcagattta gtcaggagtt taaagacaac ttggaacaga aggatagaac atggaaggag
      901 ttaattagta taacaaatgg agattattag ccatgaggcg aaccattgaa actcgttttc
      961 tgaatcatac gctttgtttg atatagaaca aacctttgcc agaggactgc tcggattaca
     1021 gctacggatt gaacaaatcc tacttatcta caatctaagc tattttgact ttaactagta
     1081 gcacgggtaa aaaaactgcc tagttcaatc agaactgggc agtacttatt ttacattcta
     1141 aaaaatggat tgaaattttt ctcatgtcca acgctggtat tttgaccatg acctggatgg
     1201 acagtatagt gatttggcag ggtaaagata tgttctctga tgccagtaat caggtcatca
     1261 aagttgctgg ttggcaggtc tgtgcgtccc acagtttcac ggaaaagtgc gtctccagtg
     1321 atgacacatt catcctctgg gaagataaag gaaacaccac caatagagtg accaggtgtt
     1381 ggaactacct tgaaatgaaa accgtcaaac tggtagtctt cttggtattg gaagattttt
     1441 tcagcaggtc ggcagactac attttccata tcatcatgac gaggtagacc tgatagattc
     1501 atctctggtg tgtagagcca gctggcttca ctttcagcca catagacagg cgggaaattg
     1561 taggtctcac gcaggatgtc caaactcata atatggtcat agtgagtgtg agtcaggaga
     1621 atagccgaaa ttggttttcc gattttttcg atagttactt gaatttttgt ccagtcactc
     1681 ccagggtcaa tgataatcag atgggtatca ttttcaaggt agtaggtatt ttgaaaagcg
     1741 acaggattga cagatttgtg aataatcatt ctttttcctt cttttaacag ttactgatgt
     1801 tactagtcta acaaaaaaac acgccttctt catggaattt gtttggagct ttttccatga
     1861 gagggaatat gctataatga acagtatgaa agacacaaga acaagcaatc ggtatgccgt
     1921 tgtagatttg gaagcaactg gtacaggtac cgatgcaaaa atcattcaaa tcgggattgt
     1981 tctcgttgaa aatggagaaa tcattgacag ttacgcaacg gacatcaatc cctatgagct
     2041 actggatgac catatcaaga atctgacagg tatcacagac caacagctaa gtcaggctcc
     2101 agattttggt caggtagcaa gtaccattta cgacatgatt ggagatgcta tttttgtcgc
     2161 ccataatgtc aagtttgatg ctaatctctt ggcagaagca ctcttttttg aaggctatga
     2221 actgttgaca ccgcgtgtag atacggtaga attatcacag ctattttttc cgacttttga
     2281 aaaatatagc ttgggaaacc ttgcagagca tttggaactt ggtttggacc aggcacacac
     2341 ggccatatca gatgccatgg ctacggcgcg cttgctcatc aaaattcaag agaaaataaa
     2401 gagcttaccc aggtctatcg ttgaaaagat tcttgatttg gcggacaatc tgctctttga
     2461 atcccgtctg gtcattgatg agatggtgcc tgttctgtca gaaaatctat catacgattt
     2521 agaatctatt catgacttgg tcttgaaaaa gccagaagaa atcaagtcag cctaccgatt
     2581 atctgaagat ttttcgacca atattgccct tctcggcttg catgaacgaa aaaaacaaac
     2641 tgcttttgct caagtagtag aaaaacgctt ggttgacgaa gaaaaagtgc attttatcca
     2701 agcgcaggca ggtttgggga agacttacgg ttatctcttg cctttacttg ctaagtcaga
     2761 ccaaccttta ttggtcaccg taccgaccaa gctccttcaa gagcagatta tggaaaagga
     2821 agggagcaag cttaaagaga tatttcgtat tcctatagtc tctctaaaat cgcccaagca
     2881 ttttatcaag ttagacaact actggaaaac cctgcagagg caggatgata atcgcctggt
     2941 caatcaattc aaaatgcagg tgcttgtctg gttatgcgaa accacgacag gtgatttgga
     3001 tgagcttaag caaaaacaac gctatcaagc ttactttgat gagattgccc acgatgggaa
     3061 attggagcct gaaagtcttt tttggggctg ggatttttgg caacgattga accaccaagc
     3121 tctctatagt agactgctca ttaccaacca cgcttatttt ctcaaccatc ttaaggatca
     3181 agaccctctc atggataagc gtctggtggc gattgatgaa gcacaaaagt ttctcttggc
     3241 tgctgaaaat ctagcgacgg cttcgcaaga tttgacaagt atgttacaag tattgcaaag
     3301 taaaaaggac agagcgacga ctattttgga ccaacgtttg tatgagtctt gccagtttga
     3361 actcaaccac tttttgagtc gttttcgcca gcatggtcaa cgagaggttc aaaaggctga
     3421 acttcatcaa cttggtcaga acctggagga gcttggagat gttgatttga acgacttgca
     3481 tcagctcata gactattacg ataccttttg gttggaggag aaaaatttag aagaaaagcg
     3541 gcttgcctat ttgcggggct ccaaggacag cctcttaaat gcgaccaact acctgccaga
     3601 tactaagatt ttctgcatca gcgcaacctt gaccattagc aaaaaggtca gcctggcgga
     3661 tctgctaggc tttgagcagg tcaccttgga tttgataccg actcaaactg tgaccaacca
     3721 gcagctgctt tttccgaccc atctccccga catcctcgct tggaccaagg aggagcatgc
     3781 agcttatttg gctactattt taggagaaat agcagagctg ggacgaccga ttttggtctt
     3841 gtttacatcg atttctatgc tattgcaggt gtcggatttg ttagaagaca ataacattcc
     3901 ccacctagcc cagcacaagc atggacagga aatgactctc aaacgcaaat ttgaacgagg
     3961 ggaatgccaa attttactcg gcacaggtgt attctgggag ggcgttgact ttgccagtca
     4021 gaatcaattg attcaggtta ttacaaggct cccatttgac aatccaaagg accggtttgt
     4081 acaaaaaatc aatcaccatc tgcgtgaaga agggaaaaat ccattttatg attacagcct
     4141 gcctatgatg atgattaagc tcaaacaagc catcggtcgt accaatcgtc atgacaaaca
     4201 agattcacta gtcttggttt tggacccgcg tgttcacacc aaacgctatg gacagcaaat
     4261 tctgtccttc tttgagaaag attattcaat gttaagtgtg gatgcgaaag aaatagaagg
     4321 agctattcaa gatttttttg aataagttga aagggagcca caaggcttcc ttttgtttcc
     4381 agcgtttttt taatgctact catgattagg aatctattcc ctctctcctt gccgaataac
     4441 agtgattttc tttatgatta aaatatg
//
LOCUS       DBNFEG010000163         4344 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.163, whole genome shotgun sequence.
ACCESSION   DBNFEG010000163 DBNFEG010000000
VERSION     DBNFEG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.163"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            16..495
                     /locus_tag="AC1PNG_001810"
     CDS             16..495
                     /locus_tag="AC1PNG_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIP6702292.1"
                     /translation="MKTTKVVLKSLVVLGAVFALAACGSKQESASTSSSTKETVVLKD
                     GTYKAESSKDDYGYKIVHTLTVKDGKISESKFDYEAEDGSLKSANEEYNKKMKDKAGV
                     SAGEAIEQLNAALVEKQELSAVEVVSGATHTSEDFKKSTEALLAAAAEGKTETVKLD"
     gene            573..1622
                     /locus_tag="AC1PNG_001811"
     CDS             573..1622
                     /locus_tag="AC1PNG_001811"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIP6702293.1"
                     /translation="MKKIASLLFVGLLMVLLVACGGKQNESLSVVKTPLTRSESLLHT
                     VVQLSIYHENQEEAMDEAVSYIKEMESLLSTNLEGADVYRINQAAGKEAVKVDERTFE
                     VIETAIDMSKESQGLFDISIGAVSNLWKIGDEDARKPSDEEIKAALPFINYKDITLDK
                     EKKTVFIKEGMTLELGAISKGYIADKVRELFASKGITTAIINLGGNVVVMGDSPTTQN
                     GWNVGVQDPDQVRGSTVGSVPGTHDSVVTSGIYERYLEVDGVKYHHILDPKTGYPVEN
                     DISGVTVFSKTSIQGDALSTTLFLLGVEKGLDFINQIDGVEAVFIDKEQGVHLSDGLK
                     ESFELTNEEYHLVNE"
     gene            1615..2571
                     /gene="menA"
                     /locus_tag="AC1PNG_001812"
     CDS             1615..2571
                     /gene="menA"
                     /locus_tag="AC1PNG_001812"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HIP6702294.1"
                     /translation="MNKSTKGLSLPVFLEFVEMKTKVASVFPMTLGILWSLYRYQAFN
                     WLNTLLFIIAVLSFDMCTTAINNSMDYHKAKDEAYRQESNVIGKFSLDFRQMIGIVLA
                     LLIFSLLVSLLLVWRTSWLLLPMGALCFLIGIFYTFGPIPLSRMPLGEVFSGVTMGFG
                     IFFLAVFIQQPDLLLTSQVSGQWMTLQFSWIKIIEIIFMSIPLVTLIANIMLANNTCD
                     LEEDIRNHRYTLVYYIGKKNALKLYFVLASLPWLLWIIYCLIGFLPIWALAGLIGVWP
                     AYKSLETFLKKQVKRETFIEAVKSFVLFALLYVVILALALIF"
     gene            complement(2879..3307)
                     /locus_tag="AC1PNG_001813"
     CDS             complement(2879..3307)
                     /locus_tag="AC1PNG_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HIP6702295.1"
                     /translation="MTFKSILKQLKLFDYILIGFTLVLSFLPAIFTYTHLTTDTNEAK
                     TIAYVRINGEVVDQFELSKDTPHQEKTYYPNEGQYNIIEVDGERIRVKEDNSPDQIAV
                     MTSWISQPGQLSVCLPHNLLIEIKSVGGESTDEEELILPL"
     gene            complement(3360..3707)
                     /locus_tag="AC1PNG_001814"
     CDS             complement(3360..3707)
                     /locus_tag="AC1PNG_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702296.1"
                     /translation="MNVDKPKFILTLIVLSYTAICSLYIHSYLTGDNPPIFILLFTML
                     PILNSDWQTSGPEANAFQKAIYLYHKPLLWFLFAGFALTFSIVFFAGKIQPLHAGFWG
                     MLAILLFFRQLKK"
     gene            complement(3697..4041)
                     /locus_tag="AC1PNG_001815"
     CDS             complement(3697..4041)
                     /locus_tag="AC1PNG_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP6702297.1"
                     /translation="MNKPFTPKQTCRFFVLFYALFSAFFIYSYMVNEPINGSFLLMLP
                     TIQIIKSYQTAEEKRYFIIQAIFALFAMTLTYSVGIWLGHVKPLILILPAGFAAAILY
                     RIYFKKEKPDER"
     gene            complement(4054..>4344)
                     /locus_tag="AC1PNG_001816"
     CDS             complement(4054..>4344)
                     /locus_tag="AC1PNG_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HIP6702298.1"
                     /translation="MRTLHADLPQYDMDELSPEEKQGQTLNPLVLGLGVALLSSLFAG
                     LADLFSLQLPLLASIFHVKSLLFGGMMGLFASLALSAIAYLQKLEADSVKKK"
BASE COUNT         1293 a          768 c         1006 g         1277 t
ORIGIN      
        1 aaaaggagca ttgttatgaa aacaactaaa gttgtgttga aaagtttggt agttcttggt
       61 gcggtatttg cactcgcagc ttgcgggtct aaacaagaat ctgcttcaac ttcgtcttca
      121 actaaagaaa cagtagtatt gaaagatggt acttataaag ctgagtcaag caaagacgat
      181 tatggctaca aaattgttca cacattgact gttaaagatg gaaaaattag tgagtcaaaa
      241 tttgactacg aagcagaaga cggaagtctt aaatcagcaa atgaagaata caacaaaaaa
      301 atgaaagata aagctggagt ttctgccggt gaagcaattg aacaactcaa tgcagctttg
      361 gttgagaaac aagaactctc agctgttgaa gttgtatcag gtgcaactca tacttctgaa
      421 gacttcaaga agagtacaga agcccttctt gcagcagctg ctgaaggaaa aacagaaact
      481 gtaaaacttg actaattaag tctatacaga aaagacacag tcgcacttgt ttggctactg
      541 tgtcttctta ttttttgggg aaggtaaaat cagtgaaaaa aattgcaagt ctattgttcg
      601 tgggcctact gatggttttg cttgttgcct gtggtggtaa acaaaacgaa agtttatctg
      661 ttgtgaaaac tccgttgact cgtagtgaaa gcttacttca cacagtagtg cagttgagta
      721 tttatcatga aaatcaggaa gaagcgatgg atgaagctgt ctcctatatt aaggagatgg
      781 aaagtctgtt gtccacaaac ttggaagggg cagatgtcta ccgtatcaat caggcagcag
      841 gaaaagaagc tgtcaaggtt gatgaacgta cctttgaagt gattgaaaca gctattgaca
      901 tgagtaaaga aagtcagggg ctatttgaca tttccattgg agctgtgagc aatctgtgga
      961 aaattggaga tgaggatgca cgtaagccga gtgatgaaga aatcaaagct gctctgccat
     1021 ttatcaatta taaagacatt actttagata aggagaagaa gaccgtcttt atcaaagaag
     1081 gaatgacctt agaattagga gccatttcca aaggttacat tgcagataag gttagggaat
     1141 tgtttgccag caaagggatt acgactgcta tcatcaatct aggtgggaat gtagtggtta
     1201 tgggggattc gccaacgaca caaaatggtt ggaatgttgg agttcaagac ccagaccaag
     1261 tgcgtggatc aacggtcggt tctgtgccag ggacacatga ttcggttgtt acctcaggta
     1321 tttatgagcg ttacttagag gtggacggtg tcaaatatca ccatatcctt gatcccaaaa
     1381 ctggatatcc tgtggaaaac gatatctcag gtgtgacagt cttttccaaa acatctatcc
     1441 aaggagatgc cttatccaca actctcttct tactaggtgt ggaaaaaggt cttgacttta
     1501 tcaatcagat tgacggtgta gaagctgttt tcatcgataa ggagcagggt gttcatctga
     1561 gtgatggctt gaaagaaagc tttgaattaa cgaatgagga gtatcatctt gtcaatgaat
     1621 aaatcaacta aagggctcag tttgcctgta tttctagaat ttgttgaaat gaagaccaag
     1681 gtagccagtg tttttccgat gaccctgggg attttatgga gcctgtatcg ctatcaagct
     1741 ttcaattggc tcaatactct cctcttcatt attgccgtcc ttagctttga catgtgtaca
     1801 actgccatca acaatagcat ggactaccac aaggccaagg atgaagccta tcgtcaggaa
     1861 agcaatgtga ttggaaaatt ctccctagat tttcgtcaga tgattggaat cgtccttgct
     1921 ctacttattt tttctctact ggtttcatta ctccttgtat ggcggaccag ttggctcctc
     1981 ctaccgatgg gagctctctg tttcctcatt ggtatcttct atacctttgg acctattccg
     2041 ctttcgagaa tgccactggg tgaagttttc tctggagtaa ccatgggatt tggcatcttc
     2101 tttcttgctg tatttattca gcaaccagat ttgctactga ccagccaagt gagcggtcag
     2161 tggatgactt tgcaattttc ttggataaaa ataattgaaa ttattttcat gtccattcct
     2221 ctggtaacct tgattgctaa tatcatgttg gctaacaaca cctgtgattt agaagaagat
     2281 attcgcaacc atcgttatac tctggtttac tatattggca agaaaaatgc tcttaaattg
     2341 tattttgtct tggcaagttt accatggttg ctgtggataa tctactgtct gataggattc
     2401 ttaccaattt gggcactggc cggtttaatc ggagtttggc ctgcttataa gagtctagaa
     2461 acatttttga aaaagcaagt caaacgggaa acctttatcg aggcagtaaa gagttttgta
     2521 ctctttgcgc tgctatatgt ggtgatactt gctttagccc ttattttcta aaaaggaaag
     2581 acacagtcgt tcataaggct gtgtcttctt tgttatatcg tcagttagtt tttcttttat
     2641 tacttcgacg agcgaggaaa catcgtttcc taatttccaa cttcaaacac tccactggag
     2701 tatttgaact cgccttgcct gattgaaaca gtctggggga ctgtttcaag tcaatgccta
     2761 gaaataagaa agcattgagt tatgcctgcg gcttttgatg cgaactttgt tcgctttatc
     2821 tccaacctcc aaagatttcc caaacctttg gagctagtac taaaagtaaa ctaaaagact
     2881 atagtggtaa aatgagttct tcttcatcag tgctttctcc gccaacggat ttgatttcaa
     2941 tcaacaggtt atgtggcagg cagacggaga gttgacccgg ctggctaatc cagctggtca
     3001 taacggcaat ttggtctggg ctattgtctt ccttgacacg aatgcgttcg ccatcaactt
     3061 caatgatatt gtattgccct tcattgggat agtaggtctt ttcttgatga ggtgtgtcct
     3121 ttgataattc aaattggtcg accacctcac cattgatgcg gacataggca attgtttttg
     3181 cctcattagt atctgtagtc agatgtgtgt aggtaaaaat tgctggaaga aaggataaaa
     3241 ctagggtgaa tccgattaag atataatcaa atagtttgag ttgtttgaga atggatttga
     3301 atgtcatata caatctattt tatagaaaaa atcctacttt gtaaaatgtt ggatgcagac
     3361 tactttttca attgtctaaa gaagagtagg atggctaaca ttccccaaaa tccagcatga
     3421 agtggctgaa tttttccagc gaaaaataca atggagaaag ttagggcaaa acctgcaaag
     3481 agaaaccaga gcagtggttt atgataaaga tagatagctt tttggaaagc atttgcttct
     3541 gggccactag tctgccagtc actattcaag attggaagca tagtgaacag taagataaaa
     3601 attggtggat tgtccccagt caggtagcta tgtatataca ggctacaaat tgctgtatag
     3661 gagagaacaa tcaaagtgag tatgaatttc ggtttgtcaa cgttcatctg gcttctcctt
     3721 tttgaaataa atccgataaa gaatagcggc agcaaatcca gcaggaagga tgaggataag
     3781 tggtttgaca tgtcctaacc aaatgcccac ggaataggtc aaggtcatgg caaacagggc
     3841 aaagatggct tgaatgataa agtagcgttt ttcttctgct gtttgatagg atttgatgat
     3901 ttggatggtc ggtagcatga gcaggaatga accgttaatc ggctcattga ccatgtaaga
     3961 gtagatgaag aaggcagaaa ataaagcata aaatagaaca aaaaatcgac aggtttgttt
     4021 gggtgtaaaa ggtttattca tggggtcagc tccttatttt ttcttgacag aatctgcttc
     4081 cagtttttgc agataggcaa tggcagacag ggctagactg gcaaagaggc ccatcatgcc
     4141 accgaagagg agggatttta cgtggaagat ggaagccagt aggggaagct ggaggctaaa
     4201 gaggtctgcc agcccagcaa agaggctaga tagcagggca actcccagtc ccaaaaccag
     4261 tggattgagg gtctgtcctt gtttttcctc agggctcagt tcgtccatgt catattgagg
     4321 caggtcagca tggagggtgc gcat
//
LOCUS       DBNFEG010000164         4335 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.164, whole genome shotgun sequence.
ACCESSION   DBNFEG010000164 DBNFEG010000000
VERSION     DBNFEG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.164"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            1..707
                     /locus_tag="AC1PNG_001817"
                     /pseudo
     CDS             1..707
                     /locus_tag="AC1PNG_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019785287.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type II restriction endonuclease"
     gene            828..2090
                     /gene="purD"
                     /locus_tag="AC1PNG_001818"
     CDS             828..2090
                     /gene="purD"
                     /locus_tag="AC1PNG_001818"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIP6702299.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESEQVEQVFVAPGNDGMTLDGIEL
                     INIGISEHSALINFAKENDIAWTFVGPDDALAAGIVDDFEQAGLKAFGPSRLAAELEW
                     SKDFAKQIMVKYGIPTAAFGTFSNFEEAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAREMLLDNKFGDSGARVVIEEFLAGEEFSLFALVNGDQFYILPTAQDHKRAF
                     DGDQGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPILEGMITEGRSYLGVLYAGLILT
                     DQGPKVIEFNARFGDPETQIILPRLTSDFAQNIDDILHKRPTQLTWLNSGVTLGVVVA
                     SNGYPLDYEKGVTLPAKTEGDITTYYAGARFAENSRALLSNGGRVYMLVTTADTVQDA
                     QEKIYSELKNQDTTGLFYRTDIGSKAVK"
     gene            2116..2604
                     /gene="purE"
                     /locus_tag="AC1PNG_001819"
     CDS             2116..2604
                     /gene="purE"
                     /locus_tag="AC1PNG_001819"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009908864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIP6702300.1"
                     /translation="MNIPISIIMGSSSDWKTMKKAADVLDKFGVAYEKKVVSAHRTPD
                     LVFRHAEEARGRGIKVIIAGAGGAAHLPGMVAAKTTLPVIGVPVQSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALTALRILSIEDSGIADQLASFAIEQGKIAEAMSDE
                     LN"
     gene            2591..3676
                     /gene="purK"
                     /locus_tag="AC1PNG_001820"
     CDS             2591..3676
                     /gene="purK"
                     /locus_tag="AC1PNG_001820"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HIP6702301.1"
                     /translation="MNSTKTIGIIGGGQLGQMMAISAIYMGHKVITLDPAADCPASKV
                     SEVIVAPYHDVAALKQLAERCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNC
                     IFEKDFLAKKAGVQVAPYKVVTSSLDLEGLDLSKNYVLKTATGGYDGHGQKVIREAAD
                     LEAASQLANSTECVLEEFVNFDLEISVLVSGNGSDYTVFPVQENIHRNNILYKTIVPA
                     RISDELAEKAKTMALQIADKLHLAGTLCVEMFVAGQEILVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHIRGILGEPLPPIRLLSPAVMINVLGQDMEAVQTFLQENPTAHAHFYGKLE
                     AKHNRKMGHVTVLTEDVEVGFRQDEIR"
     gene            3663..>4335
                     /locus_tag="AC1PNG_001821"
     CDS             3663..>4335
                     /locus_tag="AC1PNG_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HIP6702302.1"
                     /translation="MKLDRLKEVDVREIWKHEQYDFSEWLSRTENIELLSDEIGLTLT
                     EIDKEVFVGSYRCDLVAIDETTGIKVIIENQLELTDHDHLGKIITYASGLDAEVVIWI
                     VKKAREEHRSAIEWLNNNTTKNISFFLIEIHAYQIGSSMPAPKFVLVEKPNDFTKLAK
                     GNNHGGDLSKAQAERLYFWTQFNNVVATKNKPFNVRKATTDHWYDVAIGTSEAHISIT
                     LVNKSK"
BASE COUNT         1212 a          903 c         1079 g         1141 t
ORIGIN      
        1 aactttttaa taaacaacca aatttattaa aagctatacc aagtttgatt gcaagtcgtg
       61 ataaaacact ggatatttta tcaattgatg ccgatgataa tatgggtttt gataacttag
      121 attttgcaaa aattgattag gatagaattg tagattatac taactttgtg gaagcaacag
      181 gattattaga ttttttgcaa aagaaagcca atcgttcttt agtggactac gtttatggag
      241 tagaggcggg acttgacagt aatgcacgta aaaacagaag tggtactacc atggagggga
      301 ttttagagcg aacagtagca cgtgtctgta aagaatatgg attggagttt aaggcacaag
      361 caacagctcc atttataaag gataattggg ggattgaggt ccctgttgat aagtcggaac
      421 gccgatttga tgttgctgta ttttcgaaag ataaacataa attatggtta attgaaacga
      481 attattacgg tggaggcggt agcaagttaa aggctgtagc tggagaattt acagaattaa
      541 gtcaattcat cacaaaatct acagatgatg ttaattttgt gtgggtaact gatggtcagg
      601 gatggaagac tgcccatctt cctttagcag aagcatttgg tcatataacc catgtcttca
      661 atcttgacat gctaaaacaa ggttacttgc atgatttgtt tagataatca agtttgctca
      721 cctcgtgtga gctttttcca ttcccgaaca ttctcctgtt ttttctatta aaatccgtct
      781 gtttttgcta tactgtttat agaaaatccg tttttgaggt gtaagaaatg aaacttttgg
      841 ttgtcgggtc tggtggtcgt gaacacgcta ttgcaaagaa gttgttagag tctgagcagg
      901 tagaacaggt ctttgttgct cctggaaatg atggaatgac tttagacggt attgaattga
      961 tcaatatcgg tatttccgaa cattctgccc tcatcaactt tgcaaaggaa aatgatattg
     1021 catggacttt tgttggtcca gatgatgctc tggcagcagg tatcgttgat gattttgaac
     1081 aggctgggtt aaaggcattt ggtccgagtc gtcttgccgc ggagctagag tggtcaaaag
     1141 actttgctaa gcaaatcatg gtcaaatacg gcattccaac agcagccttt ggcacatttt
     1201 ccaacttcga agaagccaaa gcctacatcg aagagcaggg tgcaccaatc gtggtcaagg
     1261 cggacggatt ggcactgggc aagggtgtgg tcgtggcgga aaccgtcgag caggcggtcg
     1321 aagcggcacg ggagatgctc ttggacaaca agttcggtga ctcaggtgcc cgcgtggtca
     1381 tcgaggagtt cttggcgggc gaggagtttt ccctctttgc cctggtcaac ggcgaccagt
     1441 tttatatcct gccgacagcc caggaccaca agcgtgcctt tgacggagac caggggccca
     1501 acactggcgg tatgggggct tacgctcctg ttccccacct gcctcaaagc gtagtggaca
     1561 cagcggttga cactattgtc aagcctattc ttgagggcat gattacggaa gggcggtctt
     1621 atctgggcgt gctctatgct ggcttgattt tgaccgacca aggtcccaag gtcatcgagt
     1681 ttaacgcccg ttttggcgac ccagaaaccc agattattct gcctcgtctg acctctgact
     1741 ttgctcagaa catcgacgac atcctccaca aacgtccgac acagctgacc tggctgaata
     1801 gcggcgtgac gctgggtgtt gtcgtggcct caaatggcta tcctttggat tacgaaaaag
     1861 gcgtgacctt gccagcgaag accgagggtg acatcacgac ctattatgca ggggctcgtt
     1921 ttgcggaaaa tagcagagca ctgctttcaa acggcggtcg agtttacatg ctagtcacca
     1981 cagcagacac cgtccaagac gcccaggaaa aaatttactc ggagctgaaa aatcaggata
     2041 ctacaggcct cttttatcga acagatattg gaagcaaggc cgttaaataa ggaacaaaga
     2101 aaaggaaaga caagtatgaa tattccaatt tccatcatca tgggctctag ttccgactgg
     2161 aaaaccatga aaaaagcagc cgatgtgctg gacaaatttg gcgtagccta tgaaaagaaa
     2221 gtggtctctg cccaccgcac cccagacctc gtgttccgtc atgccgaaga ggcgcgtggt
     2281 cgtggtatta aggttattat cgcgggagcg ggtggtgcgg ctcatttgcc aggtatggta
     2341 gcagccaaga ccaccctgcc tgttatcggt gttcctgtcc aatcccgtgc cctcagcggt
     2401 gtggattcgc tctattctat cgtgcaaatg ccaggcggcg tacctgtggc aacgatggca
     2461 attggagaag cgggagcaac caatgcggct ttgactgccc tgcgtatcct gtccatcgaa
     2521 gatagcggta tagcagatca actggccagc tttgctattg aacaaggaaa aatcgcggag
     2581 gctatgagcg atgaactcaa ctaaaacaat cgggattatt ggtggcggtc aactgggtca
     2641 gatgatggcc atttctgcta tttacatggg gcacaaggtc atcacgctgg atccagcagc
     2701 ggactgccca gcttccaagg ttagcgaggt tatcgtcgct ccctatcacg atgtggcggc
     2761 cctcaaacag ctggcggagc ggtgcgatgt cctgacctac gagtttgaaa atgtcgatgc
     2821 cgacggactg gacgcggtca tcaaggacgg ccagctccct caggggacag acctcctccg
     2881 tatttcccaa aactgcattt ttgagaagga ttttttggca aaaaaagctg gcgtgcaagt
     2941 cgccccctac aaagtcgtca catctagcct agacctagaa ggtctggacc tcagcaaaaa
     3001 ctacgttttg aagacagcta ctgggggcta tgatggacat ggtcagaagg tcattcgaga
     3061 agccgcggac ttggaggcgg ctagtcagtt agccaactct accgagtgtg ttttggaaga
     3121 gtttgtgaat ttcgacctgg aaatctccgt ccttgtgtct ggcaatggct ccgactacac
     3181 cgtctttcct gtgcaggaaa atatccaccg caataatatt ctttacaaga ccattgtgcc
     3241 tgctcgtatt tctgatgaac ttgctgaaaa agccaaaact atggccctgc aaatcgcaga
     3301 caaactccat ttggcaggca ccctctgtgt tgagatgttt gtggcagggc aagaaattct
     3361 tgtgaatgaa atcgctcctc gcccgcacaa ttcaggacat tattcgattg aagcctgtga
     3421 tttttcacag tttgacaccc atatccgagg cattttaggg gagcccctgc cccctatccg
     3481 cctgctttct ccagctgtta tgatcaatgt cctcggacaa gacatggaag cagtccaaac
     3541 ctttcttcaa gaaaacccta ccgctcatgc ccacttttat ggtaaactag aagcaaagca
     3601 caatcgcaaa atgggacacg tgacggtgtt gacggaagac gttgaggtgg gatttagaca
     3661 agatgaaatt agataggcta aaggaagtag atgttagaga aatctggaaa catgagcagt
     3721 atgatttctc ggagtggctg tctaggacag agaatatcga attactgagc gatgagatag
     3781 gactgactct taccgagatt gacaaggaag ttttcgttgg ctcatataga tgcgatttag
     3841 tggctattga tgaaacaacg ggaataaaag tgattataga aaaccaatta gaattaacgg
     3901 atcatgatca tcttggtaaa atcataacct acgcatcggg cttagatgct gaggttgtca
     3961 tttggattgt aaaaaaagct agagaagagc ataggagtgc tatcgagtgg cttaacaata
     4021 atacaacgaa aaatatatct tttttcctaa ttgaaatcca tgcttatcaa ataggtagtt
     4081 caatgcctgc tcctaaattt gttcttgttg agaaaccgaa tgattttaca aagctagcta
     4141 aaggaaataa tcacggtggt gatttgagta aagcacaggc ggaacgtttg tatttttgga
     4201 ctcaatttaa taatgtagtg gcaactaaaa ataaaccgtt caacgttaga aaagctacta
     4261 cagaccactg gtatgatgtt gctattggaa caagtgaggc tcatatttct attaccctag
     4321 tcaataaatc aaaaa
//
LOCUS       DBNFEG010000165         4285 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.165, whole genome shotgun sequence.
ACCESSION   DBNFEG010000165 DBNFEG010000000
VERSION     DBNFEG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.165"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(31..1983)
                     /gene="gyrB"
                     /locus_tag="AC1PNG_001822"
     CDS             complement(31..1983)
                     /gene="gyrB"
                     /locus_tag="AC1PNG_001822"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIP6702303.1"
                     /translation="MTEEIKDLQEKAQEYDASQIQVLEGLEAVRMRPGMYIGSTSKEG
                     LHHLVWEIVDNSIDEALAGFADKIEVYIEPDNSITVIDNGRGIPVDIQEKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVYKNGQVYFQEYKRGEVV
                     ADLEVVGETDRSGTTVHFTPDPEIFTETTEFDFAKLNKRIQELAFLNRGLNLSITDKR
                     EGVEQTKEYHYEGGIASYVEYLNENKDVIFETPIYTEGEMDDITVEVAMQYTTSYHEN
                     VVSFANNIHTHEGGTHEQGFRTALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPGPQFEGQTKTKLGNSEVVKITNRLFSDAFAEFLLENPQIARRIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNDATKTELFIVEGDSAGGSAKSGRDRE
                     FQAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGADFDVSKARYQKLVIMTD
                     ADVDGAHIRTLLLTLFYRYMRPVVEAGYVYIAQPPIYGIKVGSEIKEYIQPGVNQEQE
                     LQDALERHSTGRSKPTIQRYKGLGEMDDHQLWETTMNPENRLMARVSVDDAAEADKIF
                     DMLMGDKVEPRREFIEENAVYSTLDV"
     gene            complement(2165..2704)
                     /locus_tag="AC1PNG_001823"
     CDS             complement(2165..2704)
                     /locus_tag="AC1PNG_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HIP6702304.1"
                     /translation="MTRVAVVLADGFEEIEALAPVDVFRRAHFDCQIVGLDSHEVEGS
                     HGIRVQTDQVFDGDLSSFDLIVLPGGMPGSVHLRDSESLITELQRAVASGKSVAAICA
                     APIVLDKAGLLDSRHYTCFPGKEKDIPSGILLEEDVVVDGPIITSRGAGTSLDFAYKL
                     VDLFGGDGESLAQTMVYHS"
     gene            complement(2718..3947)
                     /locus_tag="AC1PNG_001824"
     CDS             complement(2718..3947)
                     /locus_tag="AC1PNG_001824"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIP6702305.1"
                     /translation="MRKLMKNRGTIDSRVDYSLILPVFFLLVVGIISLYIAVSQDYPD
                     NVVQMVGQQIAWIGIGTVAAFLVMFFSTKFLWQITPYLYVLGLGLMVLPLFFYSPDLV
                     ASTGAKNWVTIGGMTLFQPSEFMKIAYIIMLSRVIVTFHKYYPNRKIREDFMLIGYMT
                     LFTIPVLILLALQKDLGTSLVFVAIFSGMLLLSGVSWKILLPTALTGIVLVGGFMLIF
                     ISPGGTTFLHNLGMDTYKINRIAAWLDPFKNAQSTTYQQAQSLIAIGSGGLKGLGFNK
                     TNLLIPVRESDMIFTVIGEDFGFIGGTVLIGLYLLLIYRMLRVTLKSNNRYYTYISTG
                     YIMMLLFHVFENVGAATGLLPLTGIPLPFISQGGSSMVSNLIGVGLVLSMGYQSRLAD
                     EKETNRSRRYRKITIKR"
BASE COUNT         1310 a          997 c          733 g         1245 t
ORIGIN      
        1 ctctagtata tgcacttgtt tttttagttt ttagacatcc aatgttgaat aaacagcatt
       61 ttcctcgata aactctcgac gaggttcaac cttatccccc attaacatgt caaaaatctt
      121 atcggcttct gccgcatcat caactgaaac gcgtgccatg agacgatttt caggattcat
      181 cgtagtttcc cacaactgat gatcatccat ttctcccaaa cctttgtaac gttggatcgt
      241 tggcttggac cgtcccgtac tatgtctttc taaggcatct tgcaattctt gctcttgatt
      301 aaccccaggt tgaatatatt cttttatctc acttccaacc ttgataccgt aaataggtgg
      361 ttgagcgata tatacgtatc ccgcttctac aacaggacgc atgtagcggt aaaataatgt
      421 cagaaggagt gtccgaatat gggcaccatc gacatcggca tctgtcataa tgactaattt
      481 ttgataacgt gccttgctaa cgtcgaaatc cgcaccaaag ccagttccca tagctgtaaa
      541 tagactacgg atttcttcgt tggccaaaat cttgtccatg ctggcttttt ctacgttcaa
      601 aatcttacca cgaatcggta aaatagcctg aaattcacgg tcgcgaccgg atttagctga
      661 accaccggct gagtccccct caacgatgaa aagctctgtt ttcgttgcat catttgagga
      721 acagtctgct aacttacctg ggaggtttga gatttcaaga cccgatttct tacgagttac
      781 ctcacgcgca cgcttagcag caatacgcgc cttgcttgcc aaaatccctt tttcaacaat
      841 ccgtctagca atctgtggat tttccaaaag aaattctgca aaggcatcag agaaaaggcg
      901 gttggtaatt ttgaccactt cactattccc aagttttgtc ttagtttgtc cctcaaattg
      961 aggacctggg tgcttaacag aaatgaccgc tgtcaagcct tcacgcacat cttcaccagt
     1021 caaattatcc tcattttctt tgagaatttt atttttcttg gcgtagtcgt tgataacacg
     1081 tgtcagagct gtacggaatc cttgctcatg ggtaccacct tcatgtgtgt gaatattgtt
     1141 ggcaaaacta actacgtttt catgataact cgtcgtatat tgcatggcaa cctcaacggt
     1201 aatatcatcc atttcacctt ctgtatagat aggggtctca aaaatgacat ccttgttctc
     1261 attaagatac tcaacgtagg aagcaatacc accttcatag tgatattcct ttgtttgctc
     1321 tactccctca cgtttgtccg taatagagag atttaaacca cgatttagga aggccaattc
     1381 ttggatccgc ttatttaatt ttgcaaagtc gaactcagtc gtctcagtaa aaatttctgg
     1441 atcaggtgta aaatgtaccg ttgtaccaga acggtctgtt tctccaacaa cctccaaatc
     1501 tgcaacgact tcacctcgtt tatactcttg gaaatatacc tgaccatttt tgtagacatg
     1561 gacatctagc tgagtagata gggcgtttac gactgatgaa cctacgccgt gcaatccacc
     1621 tgagacctta taaccgcctc cgccaaattt accacctgca tggagcactg taaagacagt
     1681 ttctaccgct ggacgaccag ttttttcctg aatatctacc ggaataccac gtccattatc
     1741 aataaccgtg atggagttat ctggctcaat ataaacttca attttatccg caaaaccagc
     1801 cagcgcctca tcgattgagt tgtcgacaat ttcccaaacc aagtggtgga gaccttcttt
     1861 agatgttgag ccgatataca tccctggacg cattcgaaca gcttcaagac cttccaagac
     1921 ttgaatctga ctggcatcat attcttgagc cttctcttgt aaatctttga tttcttctgt
     1981 catttctttt ccttattttc tatttgttgc tccttaaaaa tagaaaagcc aatccatctg
     2041 gataaaagcg aaacatattt ttagagcaaa aatcattcta acgaataccc tttattatac
     2101 catattttct aatattttga tagaaaaaag aagaggaaac attagctcca tcttcttcat
     2161 tactttaact atgataaacc attgtttgag ctaggctttc cccatctcca ccaaataaat
     2221 caaccaattt ataggcaaaa tccaaacttg ttccagcacc tcggctagtg ataattggac
     2281 catcaacgac cacatcctct tccagaagaa tacctgacgg aatgtctttt tctttccctg
     2341 ggaaacatgt atagtgacga ctatctaaca aaccggcctt atccagaaca attggagccg
     2401 cacagattgc tgctactgat tttcctgatg caaccgctct ttgcaattcc gtaattagtg
     2461 actctgaatc tctaaggtga acagaacctg gcattcctcc tggtaaaaca atcagatcaa
     2521 aggaagacag gtctccatca aagacttggt cagtttgaac acgaataccg tgcgatccct
     2581 ccacttcatg actatctaaa ccaacaatct gacagtcaaa atgagctcga cggaatacat
     2641 ctacaggggc caatgcttcg atttcttcaa aaccatcggc taaaacaact gcaactcttg
     2701 tcatataaac caactttcta acgttttatc gtaatttttc tatagcgacg tgatcgattt
     2761 gtttccttct catcagctag tcgtgactga taccccattg acagcactag accaacacca
     2821 atcaaatttg aaaccatgga cgagccacct tgggagataa acggcaaagg aatacctgtt
     2881 aaaggcaaca aacccgttgc agcgccaaca ttttcaaaca catggaacaa cagcatcatg
     2941 atatagccag ttgatatgta ggtgtagtat cgattgtttg acttcaacgt tacccgcagc
     3001 attcgataaa tgagcaacaa gtatagtcca atcaataccg tcccaccaat aaaaccaaag
     3061 tcctctccaa tcaccgtaaa aatcatatca ctttctcgaa caggtataag aagatttgtt
     3121 ttattgaaac ctagtccttt taggccacca cttccgatag ctatcaaact ttgagcttgc
     3181 tggtaggtcg ttgattgggc atttttaaag ggatcaagcc aggctgcaat acgattgatt
     3241 ttataagtat ccatccccaa attgtggaga aatgttgttc caccaggaga aataaaaatc
     3301 agcataaatc caccaaccaa aacgatcccc gttaaggcag tcggtaatag aattttccaa
     3361 gaaactccag ataatagaag cattccgcta aagattgcta caaatactaa ggaggttcct
     3421 aaatctttct gtaaggccaa caaaatcagg acggggatag taaacagtgt catataacca
     3481 atcaacataa aatcctcacg aatttttcga tttggataat atttatgaaa ggtgacaatc
     3541 acccttgaca acataatgat gtaggctatt ttcataaatt cggatggttg aaaaagagtc
     3601 attccaccaa tcgttaccca gttttttgct cccgttgacg ctactaagtc tggactgtag
     3661 aaaaacaaag gcaataccat aagtcctagc ccaagtacgt acaagtaagg tgtgatttgc
     3721 cacagaaact tagtggaaaa gaacatgacc aaaaaagctg ccactgttcc aattcctatc
     3781 caagcaattt gttgaccaac catctgtaca acattatccg gataatcttg actaaccgca
     3841 atgtagagag aaataatccc cacaactaat aaaaaaaaga ccggtaaaat aagtgagtag
     3901 tctactcgac tatcgattgt tcccctattt ttcatcaatt tcctcatact ttcacttcaa
     3961 attcttactc gttccattat agcagaaatc atctctcatt gaaactaaaa atctagttac
     4021 atcagcgggg atacgattta tactcaacga aaatcaaaag tagcctagga aacgaagtcg
     4081 aagatagaac tggagttcat caaggcaagt tgacaacgga gaattttgat tttcgaagag
     4141 tattactagt gcacaaatca taaaatacgt atatcttcat accttcgaac aggctgaaat
     4201 ataatgcttt accttcacac accccataag tttgctcaat tcatgagtat gtaaaccaat
     4261 ggcaatcatt ttggcaatca cgcac
//
LOCUS       DBNFEG010000166         4121 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.166, whole genome shotgun sequence.
ACCESSION   DBNFEG010000166 DBNFEG010000000
VERSION     DBNFEG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.166"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(74..991)
                     /locus_tag="AC1PNG_001825"
     CDS             complement(74..991)
                     /locus_tag="AC1PNG_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6702306.1"
                     /translation="MNDKEFGQRVRQLRESASMTREQFCDDELELSVRQLTRIEAGAS
                     KPTFSKIQYIATRLGMGLYELMPDYVSLPERYSKLKFDVLRTPTYGNEDLAEKRDAMM
                     TEIYDDYYDELPEEEKIVVDTLCSLFDVLDTDSQEYGKEILDDYLHQSYHRSKLSIND
                     LMILRLFIEHCQLEDLSVGTSNYALFTDLIEKLPQSIYDVHSESLFIVRDLFLAIVRI
                     LFSKELYNHIPVYIEKIENIMELSQDFQKKPILNLVKWKYELKVQHNHEIAERYYNEA
                     ITFASLLNQFHLKEKLQMEWEKDTQSLKR"
     gene            complement(1238..1876)
                     /locus_tag="AC1PNG_001826"
     CDS             complement(1238..1876)
                     /locus_tag="AC1PNG_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIP6702307.1"
                     /translation="MTTPTFIWDLDGTLLDSYEAILAGIQETYEQFDIPFDREEVRNF
                     ILRYSVKDLLVRDADKYGLDSDELNHVRATSLKEKNTQIPLMTGAVEILNWTAEQGIQ
                     NFVYTHKSDNAFQVLEDLGIRHHFTEILTSDSGFARKPSPEALLFLIEKYGLDKESTY
                     YIGDRLLDVETAINAGIRSINLQINGVEQNWKIVSLLDIKQILTDKSSHNNL"
     gene            complement(1878..2624)
                     /locus_tag="AC1PNG_001827"
     CDS             complement(1878..2624)
                     /locus_tag="AC1PNG_001827"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6702308.1"
                     /translation="MAEQMRRVARLFGDWPETIIWTCLEGTMGDIYVDDSQSPQSALA
                     LYGRQSFFGFLAGQPHRDLLKICEGKNIILVPQNQAWSDLIEEVYGDGVRFFTRYATK
                     KDTEFDLGHLQKLVDDLPESFDMKLIDRNLYETCLVEEWSRDLVGNYIDVEQFLDLGL
                     GCVILHKGQVVSGASSYASYSAGIEIEVDTREDYRGLGLAKACAAQLILACLDRGLYP
                     SWDAHTLTSLKLAEKLGYELDKAYQAYEWR"
     gene            complement(2624..3625)
                     /gene="ruvB"
                     /locus_tag="AC1PNG_001828"
     CDS             complement(2624..3625)
                     /gene="ruvB"
                     /locus_tag="AC1PNG_001828"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIP6702309.1"
                     /translation="MTNRILDMEQMQDEEYVERTLRPQKLNEYIGQDKVKDQLKIFIE
                     AAKLRDEALDHTLLFGPPGLGKTTMAFVIANELGVNIKQTSGPVIEKAGDLVALLNDL
                     EPGDVLFIDEIHRMPMAVEEILYSAMEDFYIDIMIGAGEASRSVHLELPPFTLIGATT
                     RAGMLSNPLRARFGITGHMEYYELADLTEIVERTADIFDMEITHEAAIELARRSRGTP
                     RIANRLLKRVRDFAQIMGDGLIDDNITDKALTMLDVDREGLDYVDQKILRTMIEMYGG
                     GPVGLNTLSVNIAEERETVEDMYEPYLIQQGFLMRTRTGRVATAKAYEHLGYPYTEK"
     gene            complement(3920..4093)
                     /locus_tag="AC1PNG_001829"
     CDS             complement(3920..4093)
                     /locus_tag="AC1PNG_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702310.1"
                     /translation="MKQTTYTLSLGGNDSGDSKVSKMEKKFEKYMEGIAERLRRSGKR
                     SIDKWFIRVRIEE"
BASE COUNT         1163 a          988 c          776 g         1194 t
ORIGIN      
        1 ataacatata atctcctttt gttttatgaa tctagtataa aataaactct agattctcaa
       61 caggacaaaa atgtcatctt tttagactct gcgtatcttt ttcccattcc atttgcagtt
      121 tttctttcaa gtgaaattga tttagtaggc tggcaaatgt aatcgcttca ttatagtacc
      181 tctcagcaat ttcatgatta tgttgtacct ttaattcata tttccatttc acaagattta
      241 gaattggctt cttctgaaaa tcttgtgata attccatgat gttttctatt ttttctatat
      301 agactggaat atgattatac aactccttcg aaaataaaat acgaacaata gctagaaata
      361 aatcgcggac aatgaataag gattccgaat gtacatcata gattgattga ggcaatttct
      421 ctatcaaatc tgtaaataaa gcataattgg aagttccaac agataaatcc tccaactgac
      481 aatgttcaat aaataatcgc aaaatcatta aatcgtttat agatagtttg gaacggtgat
      541 agctttgatg taaataatca tcaagaattt ctttaccgta ttcctggcta tctgtatcca
      601 atacatcaaa tagggaacat agggtgtcta caactatttt ctcctcctca ggcaattcat
      661 cataataatc gtcatagatt tctgtcatca tggcatctcg cttttccgcc aaatcttcat
      721 taccataagt tggggtgcga agcacatcaa acttcagctt ggaatatctt tcgggtaaag
      781 atacataatc tggcataagc tcgtaaagtc ccatacctaa acgagttgca atatactgaa
      841 tctttgaaaa agtcggcttg gaagcacctg cttcaatacg agttaattgg cgcacagaga
      901 gttccagttc atcgtcacaa aactgttcac gtgtcatact agcagattct cgtaattgac
      961 gcacacgctg tccaaactcc ttatcgttca taaaccacac ctccaaatat actttttaaa
     1021 ttatatcaaa tatattacac aaattccata tcatgctctt cttaaaacag acggctggat
     1081 tcgtttttat tatagcttga agccataaca agataaaagc ccgatgttac gggcattcgt
     1141 caagtaatca tagcactaac ctgaacgaag ttcagcggac gtctttagac atcgctggag
     1201 agaaacagct tatagctggt gtacaagcca cccgttttca caggttgtta tgacttgatt
     1261 tatctgttaa tatttgtttt atgtccaata aagaaacaat cttccaattc tgctccacgc
     1321 cattaatttg cagattgata ctacgaatac ccgcattaat agccgtttcc acatcgagca
     1381 gacggtcgcc aatatagtag gtgctttcct tatccagccc gtatttttcg atgagaaata
     1441 gcagagcttc tggacttggt ttgcgggcaa agccagaatc gctggtcaag atttctgtga
     1501 aatggtggcg aatgcccaag tcctccaaga cctgaaaggc attgtcactc ttgtgggtat
     1561 agacaaaatt ttgaatgcct tgttccgcag tccagtttaa gatttccacc gcacctgtca
     1621 tcaagggaat ctgcgtattc ttctccttca aggaagttgc acgcacatga ttgagctcat
     1681 cgctatccag accgtacttg tccgcatcac gcaccagcaa atccttgaca gaatagcgga
     1741 ggatgaaatt tcttacttcc tctcgatcaa aagggatgtc aaattgttca taggtttctt
     1801 ggataccagc caaaatggcc tcgtaagaat ctaaaagtgt tccatccaaa tcccaaataa
     1861 aggtcggtgt tgtcattcta tctccattcg tatgcttgat aggctttatc tagttcatag
     1921 cccagttttt ccgcaagttt caaggaagtc agtgtatggg cgtcccagct agggtagagc
     1981 ccgcggtcta aacaagctaa aattaactga gccgcacagg cttttgccaa acccagacct
     2041 cgatagtctt ccctagtatc cacttctatc tcaatcccag ctgaatagct ggcataggat
     2101 gaagcccctg agaccacctg tcccttatgc aagatgacac agcccagacc caagtccaaa
     2161 aattgttcca catctatata atttcccacc aaatcccgtg accattcctc taccagacaa
     2221 gtctcgtaca gattacggtc aatcagtttc atatcaaagc tttcaggcaa gtcatcaacc
     2281 agtttctgta aatgcccaag gtcaaactca gtgtcctttt tcgtagcgta tcgagtaaaa
     2341 aaacgaactc cgtctccgta aacttcttct atcaagtctg accaggcttg gttttggggg
     2401 actagaataa tattctttcc ctcacagatt tttagcaagt ctcgatgtgg ctggccagct
     2461 aaaaaaccaa agaagctctg ccgtccatag agagctagag ctgactgggg tgactggctg
     2521 tcatcaacat aaatgtctcc catagtacct tctagacagg tccagattat cgtttcaggc
     2581 cagtctccaa ataaacgagc tactcgtctc atctgctctg ccattatttt tccgtatagg
     2641 gatagcccag gtgttcgtaa gccttggctg tcgcaacccg ccccgtccgt gtccgcatga
     2701 ggaagccctg ctgaatcagg taaggttcgt acatgtcctc caccgtctca cgctcctcgg
     2761 caatattgac cgacagagtg ttgaggccga cgggaccgcc gccgtacatc tcaatcatgg
     2821 tgcggagaat cttctggtcc acgtagtcca gcccctcgcg gtccacatcc agcatggtca
     2881 gggccttgtc cgtaatgtta tcatcaatca ggccgtctcc cataatctgt gcgaaatccc
     2941 gcacccgctt gagcaagcgg ttggcgatac ggggagttcc acgagaccga cgagccagct
     3001 caatagcagc ttcatgagta atctccatgt caaagatgtc cgctgttcgc tcgacaatct
     3061 ccgtcaaatc agccagttca taatactcca tatgaccggt aataccaaaa cgggcccgca
     3121 gaggattgga tagcataccc gcacgagtgg ttgctccaat cagggtaaac ggtggcaact
     3181 ccaaatgcac ggagcgactg gcctcccctg ctccaatcat aatgtcgatg tagaaatcct
     3241 ccatagcaga gtagagaatt tcctcgaccg ccatgggcat acggtggatt tcatcgataa
     3301 agaggacgtc gccaggctcc aagtcgttga gaagggccac caagtcaccc gctttttcaa
     3361 taacaggacc actggtctgc ttgatattga cgcccaattc gttggcaatg acaaaggcca
     3421 tggtggtctt ccccagaccc ggtgggccaa aaagaagggt atggtccaag gcttcatcac
     3481 ggagcttggc cgcctcgata aagattttga gctggtcctt gaccttatcc tgaccgatgt
     3541 attcatttaa tttctgcgga cgcagggtac gctcaacata ctcctcgtcc tgcatttgtt
     3601 ccatatctaa aattcgattt gtcatgaaac cattatagca taaaatgaaa ataccagact
     3661 cagtgagtcc agtattctca ggagattata taatccctag tccttgtcgc ttgcgactta
     3721 gagatacgga ttgaaaagat atgccagccg tttgagcttt agctcttacg gatgacgata
     3781 ttgaaactat ttcagtaaaa gtctcgctat ttctagcttg aataaagtat aactcgccaa
     3841 acttaaggga aactaaaaga gaattcattc tatttttcac tatttataaa aacgtctgtg
     3901 aattttggtc atagctactt tactcctcaa tccgcacacg gataaaccat ttatcaatac
     3961 tcctcttccc agaacgccgt aaccgctctg cgatcccctc catatatttt tcaaactttt
     4021 tttccatctt tgaaactttc gaatccccgc tatcatttcc tcccaaagat aatgtgtaag
     4081 ttgtttgttt cattctcttc tcctatctat ttattgtgca a
//
LOCUS       DBNFEG010000167         4042 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.167, whole genome shotgun sequence.
ACCESSION   DBNFEG010000167 DBNFEG010000000
VERSION     DBNFEG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.167"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            193..420
                     /locus_tag="AC1PNG_001830"
     CDS             193..420
                     /locus_tag="AC1PNG_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6702311.1"
                     /translation="MTQWTLKACRVNAGYTLRQVAKKVGKNFQTISKYEKDSTIIPFE
                     LLKDLSKLYRVKLDDIFLGDSTKKIELTPEE"
     gene            439..1185
                     /locus_tag="AC1PNG_001831"
     CDS             439..1185
                     /locus_tag="AC1PNG_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HIP6702312.1"
                     /translation="MRRNYSKVIEEMRTIHGLNLVAIGQRIGTDPRTVGKWAQGKHQP
                     NKDSRKKINNLYREVKQSMTTQTTIEPFEDFYDWSKMKKKKPTTEVIATQQFGNKSFE
                     IYGNKDNPLFIAVEVAEMIEVQNTTDLLKRIDEDEKLTYVISRAGQKREVNMLTEFGL
                     YEVLFQSRKPKAKEFKKVVKNILKEIRVNGYYMQGELIQDQPTQSAIELNSDMAYIKN
                     RLAELQSMTTMADIKIGLAKTYRIAELMED"
     gene            1198..1824
                     /locus_tag="AC1PNG_001832"
     CDS             1198..1824
                     /locus_tag="AC1PNG_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HIP6702313.1"
                     /translation="MELVYMDGRKEPYTLSSIVAECANISHHAVQEHIRKQKDRLERF
                     GKVSFQMRPLPSGQQAKDYILNEQQATLLITFLKNTEQVANFKENLVKAFFEMRDELA
                     QFRYQRALEKPKRKALHEAIETWQEAPKHAHSTVTNLLLKGTTGMNKRQLMAHRGGHN
                     GIDSLTSQELIRYQALEDMAIAMINLGMTYQDIKNMVFRPLKNAPQGA"
     gene            2263..2652
                     /locus_tag="AC1PNG_001833"
     CDS             2263..2652
                     /locus_tag="AC1PNG_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702314.1"
                     /translation="MKDNNRETIIHFEMSNQEYAPVKNAISEELKTVISKVYQLDHET
                     GWTLHYLTEIMLNHFHEDVARVQYGDLTPIECSLNSIASRVEKVRIELLKAGYEDKIE
                     VGNPMWYLKLALQDFEQMKKEMKKCKK"
     gene            2640..2843
                     /locus_tag="AC1PNG_001834"
     CDS             2640..2843
                     /locus_tag="AC1PNG_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702315.1"
                     /translation="MQEMTIETALTLIAIFTPLNLYLWFGVGLGTFRLDIEPKIKTEG
                     KYTKPIENERYGAYIQLAGKRYN"
     gene            2855..3004
                     /locus_tag="AC1PNG_001835"
     CDS             2855..3004
                     /locus_tag="AC1PNG_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702316.1"
                     /translation="MLTFRELEHIAETILKHTTPEEMQCYLDMEHDSKLLWIKYKIAS
                     LEVQA"
     gene            3001..3339
                     /locus_tag="AC1PNG_001836"
     CDS             3001..3339
                     /locus_tag="AC1PNG_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702317.1"
                     /translation="MTENRLSPHLYKVFKLLPLGMDLPITGADMERLTGLDVRTIREH
                     IRQLIVDYGIPVCGGRDNKLGGYYIPQNEVERLAGVLPLQRQYDQEHKRIHALLTADL
                     QDWRKYRDEA"
     gene            3329..3613
                     /locus_tag="AC1PNG_001837"
     CDS             3329..3613
                     /locus_tag="AC1PNG_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HIP6702318.1"
                     /translation="MKLELTAQSEADLKTGILELIENYLEAREQTPPRLLGLITAQQV
                     KDELGIKDKTLKRWEDNGLRRYQPPLEDTRKIFYRVSDILVFLGVENGKN"
     gene            3600..>4042
                     /locus_tag="AC1PNG_001838"
     CDS             3600..>4042
                     /locus_tag="AC1PNG_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HIP6702319.1"
                     /translation="MAKTKIYFWLKIDKKFFDNIFIKRLKTIPGGYTMTVIYIRLMLE
                     SLESDCILYYEGYFENLKEELALKLDVSEDDIDMTMAYFTKCGLIQIDEDKNAELPQA
                     KAMVMSETNWASYKREQRQNKERLDNVQKSLTNSNSCPTEIEIEK"
BASE COUNT         1482 a          764 c          867 g          929 t
ORIGIN      
        1 ttctctattt tttatatcct gtgggctatt ctttgccatg taatcccctc cgtttcaaca
       61 tttatctatt ttgattataa cataaaaaat gacaaggtac gaaaaaaata aaacttttgt
      121 aaaaaaacac ttgactaagt acgaaaataa tagtactatt gttttatcac ttttggaagg
      181 agctaaaaga gtatgacgca atggacgctg aaggcgtgtc gagtaaatgc aggttatacc
      241 ttgcgacagg tagctaaaaa agtaggcaag aattttcaaa ctatctcaaa gtacgaaaaa
      301 gatagtacta taatcccttt tgagttgttg aaagacttgt ccaagttgta tcgtgttaag
      361 ttagatgata tttttttagg cgacagtacg aaaaaaatag aactaacgcc agaggaataa
      421 gaaaggagca ggcaagcaat gagaaggaac tatagtaaag tcattgaaga aatgcgaacc
      481 attcacgggc tgaacttggt tgctattggt cagcgtatcg gaacagaccc ccgaacagtt
      541 ggcaagtggg cacagggcaa acaccaaccc aacaaggaca gcagaaagaa aatcaataat
      601 ctatacagag aggtaaaaca gagtatgaca acacaaacca caattgaacc attcgaagat
      661 ttttacgact ggtcaaaaat gaagaaaaag aaaccgacta ccgaggttat cgctactcaa
      721 caattcggaa ataagtcatt tgaaatttat ggaaataaag acaacccact ttttattgct
      781 gttgaagtgg ctgaaatgat tgaagtacaa aatacaaccg acttattaaa aaggattgat
      841 gaagatgaga agctgaccta tgtaatatct agggcaggtc aaaaacgaga agtaaacatg
      901 ttaactgagt ttggacttta tgaagtactt tttcaatcac gcaaaccaaa agccaaagag
      961 tttaaaaaag tagtcaaaaa catcttgaaa gaaattcgtg taaacggcta ctacatgcaa
     1021 ggtgaactaa ttcaagacca accaacccag tcagctatcg agctaaattc tgacatggct
     1081 tacatcaaga accgacttgc agagttgcaa agcatgacca ccatggcaga tattaaaatt
     1141 ggactggcca aaacataccg aattgctgaa cttatggaag actagaaagg gacaagcatg
     1201 gaattagttt acatggacgg acggaaagag ccgtatacat tgagcagtat tgtagcagaa
     1261 tgcgccaaca tcagccatca cgcagtacag gaacatatca gaaagcagaa agataggctt
     1321 gaacgatttg gaaaggtctc atttcaaatg cgacctttac cgagtgggca acaggcaaaa
     1381 gattatatct taaacgagca acaggcaact ttgctaatca cttttctaaa gaataccgag
     1441 caagttgcaa actttaagga gaacctagtc aaagcattct ttgaaatgcg ggacgagctg
     1501 gcacaattcc gatatcagag ggcactagag aagcctaagc gtaaggcact acatgaagct
     1561 attgaaacat ggcaggaagc cccaaaacac gcgcacagca cggttacaaa ccttttgcta
     1621 aagggaacta ctggaatgaa caaacgccag ctaatggcac accgtggcgg acataatggt
     1681 attgacagcc taaccagcca agagcttatc agataccaag cattagaaga catggctatt
     1741 gctatgatca acttaggcat gacataccaa gatattaaaa atatggtatt cagaccacta
     1801 aaaaacgcac cacagggcgc gtgagattaa caaaaaaagg cttaccgaga ccaatcagca
     1861 aagcctttta accactaact aaaacaaaat taacaagcag gcaagctgtt attaaaaggg
     1921 ttttagtaaa gatttttata gctagattat accatatcta ggacattatg accatacaga
     1981 gggcgctaac ccttaaaact ggagcagaaa agtattaggt gctggtatcg ccattagcga
     2041 aagctaaacc accctaagca aactacacac agcaaggcta ttattttggc acaggcttac
     2101 acgaccacag ggcaacctgg taagtctggg gcggttatcc gctgggaata gtctaggcta
     2161 ggtatagaaa tcgtatagta aagaaaccaa gccttttaca cacgggaaaa gcccactagg
     2221 gccattacac agacacaata aaagaaacga ggcaagaact acatgaaaga caataacaga
     2281 gaaacgataa tccattttga aatgtctaac caagaatacg cacccgtaaa gaatgcaata
     2341 tcggaagagc tgaagacagt catcagcaaa gtgtaccaac tagaccatga aacagggtgg
     2401 acattgcact atctaaccga aatcatgctg aaccactttc atgaggatgt tgcacgagtt
     2461 caatatggcg atttgacacc gattgagtgc agtttgaaca gtatagcctc acgagttgaa
     2521 aaagttagaa tagaactctt aaaagctgga tatgaagaca agattgaagt aggtaatcct
     2581 atgtggtact tgaaactagc cttacaagat tttgagcaaa tgaaaaagga gatgaaaaaa
     2641 tgcaagaaat gacaatcgaa acagctttaa ctttgatagc aatctttaca ccgctgaacc
     2701 tctatctatg gtttggcgtt ggtttgggca cttttcggct tgatatagag cctaaaatca
     2761 agaccgaggg taaatatacc aaacctattg aaaacgagcg ctacggggct tatatacagc
     2821 ttgcaggcaa acgctataac taggagggga aaccatgctg acatttagag aacttgaaca
     2881 tatagcagag accattctca aacacacaac accagaagaa atgcagtgct atcttgatat
     2941 ggaacacgat agtaaattgc tttggataaa atacaaaatc gcaagtctgg aggtgcaggc
     3001 atgacagaaa atcgactatc accacaccta tacaaagttt tcaagttact accgcttgga
     3061 atggacttgc ctatcacagg ggcggacatg gaacgactga caggcttgga cgtccgaacc
     3121 attagggaac atatccgcca gctaatagtt gactacggta ttcccgtttg tggtggacga
     3181 gataacaagc tagggggcta ctatatcccc cagaatgaag tagaacgact tgcaggagtg
     3241 ctaccgcttc aacgacaata tgaccaagaa cacaagcgta ttcacgcgct actgaccgca
     3301 gacttgcaag actggaggaa gtacagagat gaagcttgaa ttaaccgcac agagtgaagc
     3361 agacctaaaa acgggcattc tggagcttat agagaactat ctggaagcgc gtgagcaaac
     3421 accgccaaga ctgttagggc taatcaccgc ccaacaggtt aaagatgaac taggcataaa
     3481 agataagacc ttgaaacgtt gggaagataa tgggctaaga cgttaccagc ccccactaga
     3541 agacactagg aaaatctttt atagggtcag tgatattctg gtatttttgg gggttgagaa
     3601 tggcaaaaac taaaatatat ttttggttga agattgataa aaaatttttt gacaatattt
     3661 tcatcaagag actaaagact attccaggcg gttacactat gacagtaatc tatatccgcc
     3721 taatgcttga aagtcttgaa agtgactgca ttctctacta cgaaggttat tttgaaaatc
     3781 tcaaggaaga attggctttg aagttggatg tgtcggaaga tgatatcgat atgaccatgg
     3841 catactttac aaaatgcggt ctgatacaga ttgatgaaga taaaaatgca gagttaccac
     3901 aggccaaagc tatggttatg agtgaaacaa actgggctag ctacaaacgg gaacaacgac
     3961 aaaacaaaga gagattggac aatgtccaaa agtctttgac taattccaac tcgtgtccaa
     4021 cagagataga gatagagaaa ga
//
LOCUS       DBNFEG010000168         4027 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.168, whole genome shotgun sequence.
ACCESSION   DBNFEG010000168 DBNFEG010000000
VERSION     DBNFEG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.168"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..336)
                     /locus_tag="AC1PNG_001839"
     CDS             complement(<1..336)
                     /locus_tag="AC1PNG_001839"
                     /inference="COORDINATES: protein motif:HMM:NF028198.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwqH-like family protein"
                     /protein_id="HIP6702320.1"
                     /translation="MSKDAEIARCNQRILTTNSQISSTESTIATYKTKLEALKKAYEA
                     MKTTKSDFKDKKKSLSQLQRSADTSWSGNLFNTKYDKPLEDLISDVNDVIGKIDDNLD
                     RINNKINDYE"
     gene            complement(350..856)
                     /locus_tag="AC1PNG_001840"
     CDS             complement(350..856)
                     /locus_tag="AC1PNG_001840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702321.1"
                     /translation="MNKTWLYLLIFLPITVPFCFCLYRWRREYVIRKYSTSTIIGKVV
                     AHREWISLRPPIIEYEVKGIIYRKALEYTSVVYGPKDISYPRDIEELRNYMLERKKLY
                     NIGLMSYNMYEIFPIGYEMTVMYNPKRPQLAFVERYAGGELFFRFVSLFYFLVIILIC
                     LFLFFVLI"
     gene            complement(859..1398)
                     /locus_tag="AC1PNG_001841"
     CDS             complement(859..1398)
                     /locus_tag="AC1PNG_001841"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702322.1"
                     /translation="MKNIILIVTSMFLLTACGNSSTNTETQSSVESSTSSSVEDKALT
                     LVHGKNTTQAGEVVQYVEDLLEKNPSLGAENAISLNYADATNSDGTTTYALFLVTNRT
                     ADRIEQNFDFTVNWSYDGTVIYENQKVLYEPDEYGVLEPNTTAIIFLPLPAESVDVVK
                     NMTDNSKISLSISDLAYVE"
     gene            complement(1412..2791)
                     /locus_tag="AC1PNG_001842"
     CDS             complement(1412..2791)
                     /locus_tag="AC1PNG_001842"
                     /inference="COORDINATES: protein motif:HMM:NF016617.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T7SS effector LXG polymorphic toxin"
                     /protein_id="HIP6702323.1"
                     /translation="MTKKLDNTAISTLKTEFEASYSTAETSLYEIFKALSVLTQSVNF
                     RGAGAESHKLFLQEASLNYAYVLLEVTQTFKSYISEVEKGFLEYESEVSGKVNTDTVS
                     EYKTSLSGIAKQIELLKSDLTSPESIARSHGVSIVSTGAGNLATQFSTIDSNLEKVNQ
                     DLADTDSSLKGKATTVSEAISNLKSSLDSISSTYVTSTGKYNTSTIGQLTKQDWYLKG
                     NKDIFSDKQKSDPFVYNAGSASVLEGQYAVGLAKDTYLTTTGAVASGQYKAGIDGGEI
                     YLKSNGYVLGGSLEVQATSAIEVTAAASMASGNVDLRTGKYGWYASGGASGPSAQGKV
                     GIIDGLFSVSGQARFLFAEGTIGSYDNGEEFYYGFDASASLTEASANFDLFNVQIDGQ
                     KPVFAISAEPVLTGGGGATAAISGQSVYEDFLGLDWLDVDAVTFKVGGKAIIGGNISV
                     TIPWPDLNF"
     gene            complement(2793..3077)
                     /locus_tag="AC1PNG_001843"
     CDS             complement(2793..3077)
                     /locus_tag="AC1PNG_001843"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702324.1"
                     /translation="MSEQILINPEGFYPEIDNLSAAQALITDEMFSIDKGELNLDTVS
                     EIDSLVKELNSLYVLYREHLGRDVKNLNVVIAEWMKVDASLAGKNLLGGD"
     gene            complement(3070..>4027)
                     /locus_tag="AC1PNG_001844"
     CDS             complement(3070..>4027)
                     /locus_tag="AC1PNG_001844"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702325.1"
                     /translation="LLFLAKYKEEFAKEFYNKKINISVYASQTIFSFISSSKKLIGDI
                     DIFEKIKSKVPSREEFPELFKQFKLDYTNNFSDEVKEEIIYSEFLHYHKRFSMDNFHK
                     SIESLTFEDVEEVQKYLFQKIRYKVYIYGNVEERIEDIQAWSVNGCSEFINPIILPLT
                     DEEVVWKGIELNGISDRRSYLMHTNLFQIPIADAYLTYQILSQWYSDKKIQITIDSIE
                     MGAILPNTDNIEDFKLDLASIQLFKDNILRQYEYLSGRKQLFYSEYFMKLWLAGLDYL
                     DFYLYLQKISSEEIEDKMSTVLKVSQFCKVELSKQNMEIIDE"
BASE COUNT         1238 a          831 c          584 g         1374 t
ORIGIN      
        1 ctcatagtca tttatcttat tgttgatacg atctaaatta tcatcaattt taccaataac
       61 atcgttaaca tctgagatca aatcttcgag tggtttatca tattttgtat tgaataaatt
      121 tccagaccaa ctcgtgtccg cgctcctctg taattgactc aatgatttct ttttgtcctt
      181 aaaatcagat ttggtcgtct tcatagcttc ataagctttt ttaagtgctt ctagttttgt
      241 tttgtaagtg gctattgttg attctgtact tgaaatctga ctattagtag ttaatatccg
      301 ttgattacaa cgagcaattt cagcatcttt tgacattaga aatacctcct tatattaaaa
      361 caaaaaacaa gaacaaacag ataaggatta ttacaagaaa atagaataag ctgacaaatc
      421 taaaaaataa ctctccccca gcataacgtt ctacaaaagc taactggggt cgcttcggat
      481 tgtacatcac tgtcatttca tatccaatag gaaatatttc atacatattg tacgacatta
      541 aaccaatgtt gtataacttt ttcctttcca acatataatt tcggagttct tctatatcac
      601 gaggatatga aatatctttt ggaccataaa caacagatgt gtattctagg gcttttctat
      661 atataatacc ctttacttca tactctataa ttggaggacg aagtgaaatc cattctctat
      721 gcgcaacaac tttaccgata attgtagaag tactgtattt tcgaataaca tattccctac
      781 gccatctata aagacaaaaa caaaaaggta cagttatcgg caaaaatatt aaaagatata
      841 accaagtctt gttcattttt actccacata agccaaatcc gaaatcgaaa gtgaaatttt
      901 gctattatct gtcatatttt ttacaacatc aacactctca gcagggagag gtaggaagat
      961 aatggctgtc gtattgggct ccaacactcc atattcatca ggctcataca gcactttttg
     1021 attttcatag atgaccgttc catcatagga ccagttaacc gtaaaatcaa aattttgttc
     1081 aatcctatct gccgtcctgt tcgttactaa gaacagggcg taggtagtgg ttccatctga
     1141 attcgtagca tccgcataat ttaaactaat ggcattttct gctcctaagc taggattctt
     1201 ttcaagcaaa tcctcaacat attgaacaac ttcaccagct tgagttgtat tttttccatg
     1261 tactaatgtc aacgccttat cctccactga actacttgta ctactttcaa cacttgattg
     1321 tgtttctgta tttgtactcg aatttccaca agctgttaat aagaacatgc ttgtgacaat
     1381 taagataata tttttcatga tttcttactt tctaaaaatt gaggtctggc caaggaattg
     1441 taaccgagat attcccacca attattgcct ttccacctac tttgaatgtc actgcatcaa
     1501 catctaacca atctagccct aaaaaatctt catatacaga ttgacctgaa attgcagccg
     1561 tcgctccgcc tccaccagtt aaaacaggtt cagctgaaat tgcaaaaact ggcttctgtc
     1621 catctatttg tacattaaat aaatcgaaat ttgcacttgc ttcagtaagg ctggcactag
     1681 catcaaatcc gtaatagaat tcttcgccgt tatcatacga accaattgtc ccctctgcga
     1741 aaagaaacct agcttgacct gagacagaaa atagcccatc tataattcca acttttcctt
     1801 gtgcagatgg tccacttgca cctccgctag cgtaccatcc atattttccg gtgcgcaagt
     1861 ctacattacc tgatgccata ctagcagctg ctgttacctc aatagcagaa gttgcttgaa
     1921 cttctagact acctcccaaa acatatccat tagattttaa atatatttct ccaccatcaa
     1981 tccctgcttt gtattgtcca gatgctacag ctcctgttgt agtaaggtat gtatccttcg
     2041 ccaaaccaac cgcatactgc ccttccaaca ctgaggccga ccctgcattg taaacaaagg
     2101 ggtccgactt ctgtttgtcc gaaaatatat ccttgtttcc ttttagatac caatcttgtt
     2161 tcgttaactg accaatagtt gaggtgttgt atttacctgt tgaagtgacg taggtagaag
     2221 aaatgctatc taaacttgat ttcaaattag aaattgcttc ggaaactgta gttgctttcc
     2281 ctttcaagga actatccgta tctgctaaat cttggttcac tttctctaaa ttagaatcaa
     2341 tcgtagaaaa ttgagttgct aaatttccag caccagttga aacaatgctc actccatgac
     2401 ttctcgcgat tgattctgga gaagtcaaat cactttttag taattctatc tgcttggcta
     2461 tacctgacaa agaagtctta tactcagaaa ctgtatcagt attcactttt cctgaaactt
     2521 ccgactcata ctccaagaat cccttctcaa cttctgagat ataggattta aatgtctgcg
     2581 tcacctctaa taaaacatag gcgtagttta aactggcttc ttgtaaaaac aatttatgag
     2641 attctgcacc tgctccacga aagttcactg actgcgttaa aacggacagt gctttgaaaa
     2701 tctcataaag actggtttcc gcagtggagt atgaagcttc aaattctgtc ttaagggttg
     2761 atatagcagt attatctaac ttctttgtca tcttaatccc ccccaagcaa attttttcct
     2821 gcaagcgagg catctacctt catccactct gcaattacta catttagatt ttttacatcc
     2881 cgacctagat gctctctata aagtacataa agtgaattca attcctttac taaactgtca
     2941 atctctgaaa ctgtatccag attaagctca cccttatcta ttgaaaacat ttcatccgtt
     3001 ataagagctt gagccgctga caaattatct atttctggat agaatccctc tggatttatt
     3061 aatatttgct cactcatcta ttatctccat attctgtttt gatagttcaa ctttgcaaaa
     3121 ttgacttacc tttagtacag tagacatttt atcttcaatc tcctccgaac ttattttttg
     3181 taagtacaga tagaagtcaa ggtaatctaa accagccagc catagtttca taaagtattc
     3241 agaataaaat aattgctttc ttccgcttaa atactcatat tgccttaaaa tattgtcctt
     3301 aaatagttgg atactggcta ggtctagttt aaaatcttca atattatcag tatttggcag
     3361 tatagctccc atttctatag aatcaatggt aatttgtatc tttttatcac tataccattg
     3421 acttagaatt tgataggtca agtaagcatc tgcgattgga atttggaata aatttgtatg
     3481 catcagataa cttcgcctat ctgatattcc attgagttca atacctttcc agacaacctc
     3541 ttcatcagta agcggtagaa tgattggatt aataaactct gaacaaccgt ttaccgacca
     3601 agcctgtata tcttcaatcc tttcttcaac atttccataa atatatactt tatatcgaat
     3661 tttttgaaaa aggtattttt ggacttcctc tacatcttcg aatgtaagac tctcaatgga
     3721 tttatgaaaa ttatccattg agaaccgttt atgataatgt agaaattctg agtaaattat
     3781 ttcttctttc acttcatcag aaaagttatt tgtgtaatct agtttaaatt gtttaaataa
     3841 ctccggaaat tcttctcgtg acggaacttt tgactttatt ttttcaaaaa tatctatgtc
     3901 accaatcaac ttctttgaac tactaatgaa tgaaaaaatc gtttgagaag cgtacacaga
     3961 gatattaatt ttcttattat agaattcttt agcaaattct tctttatatt tagctaagaa
     4021 aagcaat
//
LOCUS       DBNFEG010000169         4009 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.169, whole genome shotgun sequence.
ACCESSION   DBNFEG010000169 DBNFEG010000000
VERSION     DBNFEG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.169"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..810)
                     /locus_tag="AC1PNG_001845"
     CDS             complement(<1..810)
                     /locus_tag="AC1PNG_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HIP6702326.1"
                     /translation="MEKQTFITLEDIDTSIAAENFNRLLDENKTYFLNGSWGSGKTTF
                     LESTESQSKKKFVTIDLWRNTDKRSLVEVAYASLHPVLYWGALLFSFLFVIISILMTN
                     TVNLGLSKFLHPIWMLPVGFISLVVAAVQLVAIKTEIVYSWLLANLCLKNKVLVIDDF
                     DRLTLSQQEEAYRLFSLLRGRLPIVFVGDIENIYSVKDNFLSKIIDRRVELPFVLQSE
                     QIWDDYFIKLEQRFATEISDSFKKRVKVEKRSLRDREHFNDYVNQEFFSRGK"
     gene            complement(953..1690)
                     /locus_tag="AC1PNG_001846"
     CDS             complement(953..1690)
                     /locus_tag="AC1PNG_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702327.1"
                     /translation="MNETRFLELFNDLDKVLRKVCGVADGEYADVGSMLTKAKELTRH
                     NPVEANWDKLYVARQLRNLMVHEKRTGLKEVAQPSQELITVLEKVIAQYQEPITIADY
                     LQKTQKIKPVIFEGKDKLIKALEIVEQEHFSKFPIFSDKVYQGLVSDKGLTNWLAKAS
                     KETGSIREKLVEASLEDVLACEEDSIQVIVLPITAHLYQVINHFEGRKRTTILVSRQA
                     SGKLHSPEDLVGIVTAQDLAEIYGMVD"
     gene            complement(1692..2726)
                     /locus_tag="AC1PNG_001847"
     CDS             complement(1692..2726)
                     /locus_tag="AC1PNG_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIP6702328.1"
                     /translation="MKTLDYIVTLTNTPSPTGFTTDIMNYIKAEVESFGYAASKTAKG
                     GVLVTVPGENDQEHRMVTAHLDTLGAMVRAVKPDGRLKMDLVGGFGYPSIEGENCLIH
                     CAKNGKTFTGTILMHQTSVHVYRDASTAERNQTNMEIRLDEKVTNADETRTLGIEVGD
                     FISFDPRTVVTETGFIKSRHLDDKVSAAILLHLLKVYKEEGVTLPYTTHFYFSNNEEI
                     GYGANSSIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDGSGPYHYELRQHLVALAE
                     RDGIPYKLDIYPYYGSDASAAMRAGADVKHALLGAGIESSHSYERTHLDSVVATERMV
                     DAYLKSAMVD"
     gene            2791..3747
                     /gene="birA"
                     /locus_tag="AC1PNG_001848"
     CDS             2791..3747
                     /gene="birA"
                     /locus_tag="AC1PNG_001848"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HIP6702329.1"
                     /translation="MKTYQKIYLLLKEREDYVSGEDLAQELGISRTSIWKAIRQLETH
                     GLTIEAARNRGYRLAEGDLLLPELISQELQLPVHLNMDSDSTQLDAKQGIESGHTSPA
                     LYLAPYQNKAKGRFGRPFYASKSGGIYMSLRLSPNVPFLEFKPYTILAAAAVVKAIQS
                     LCDLDVQIKWVNDIYLGHKKVAGILTEAISSMENQRVTDVIIGVGINVRIDDFPKELQ
                     QSAGNLFEEQPPFTRNQLITAIWKAFLETDEKELIALYKEKSLVVGQQVSFVENQVEF
                     KGTAIAVTDTGNLVIQLDNGKAKIISSGEISLTSWSASPPNE"
     gene            complement(3713..3901)
                     /locus_tag="AC1PNG_001849"
     CDS             complement(3713..3901)
                     /locus_tag="AC1PNG_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HIP6702330.1"
                     /translation="MKLPQFLFLAITTILAVYFMNASILTGDFLIAGIYAFIAYRNLH
                     FAYKVTKFIRLVEKQTKK"
BASE COUNT         1211 a          915 c          708 g         1175 t
ORIGIN      
        1 ttttcctctg gaaaaaaatt cttggttaac atagtcattg aaatgctctc tatcccttag
       61 acttctcttt tctaccttta ctcttttttt gaaactatca gaaatttcag tggcaaatct
      121 ttgttctaac tttataaaat aatcatccca aatctgttca gattgaagga caaacggcaa
      181 ctctaccctc ctatcaataa tttttgatag aaaattatct tttacactat aaatattctc
      241 gatatcacca acaaagacta taggcaacct ccctcttaac aaggaaaata atctatatgc
      301 ttcctcctgt tgtgagagcg ttaaacggtc aaagtcgtca atcactagaa ctttattttt
      361 taagcacaaa ttagccaata accaagaata aactatttct gtctttatcg ctactaattg
      421 caccgccgca actactaggg aaataaaccc aacagggagc atccatatgg ggtgtaaaaa
      481 tttacttaat cctagattta ctgtattggt cattaaaata gagatgatta caaaaagaaa
      541 actgaatagt aatgctcccc agtataaaac aggatgtaaa ctggcataag caacttcaac
      601 aagacttctt ttgtcagtat ttctccataa atcaatagta acgaacttct tttttgattg
      661 cgactctgtt gattctaaaa acgttgtttt tcctgatccc cacgaaccat tcaaaaagta
      721 tgttttattt tcatccaaaa gtctattaaa attttctgct gcaatagagg tatcaatatc
      781 ttctaaagtg ataaaagttt gtttttccat attttttcct aaaaaatatt tatattgtga
      841 caatgtagaa ttgggactcc taaatatctt ttatttacga gtttcacaac atattcaagt
      901 ctttttcaac acagtaatac accttgataa tggattaaca agaactattg cttcaatcca
      961 ccatcccata aatctctgcc aagtcctggg ctgtgacaat accaacaaga tcctctggcg
     1021 aatgaagctt cccactcgcc tgacgactga ctaggatagt tgtgcgctta cgcccctcaa
     1081 aatgattaat tacctgataa agatgggctg ttattggtag aacaattacc tgaatactat
     1141 cttcctcaca agctagtacg tcttcaagac tagcctccac caatttttca cgtatagagc
     1201 ccgtttcctt gcttgccttg gccaaccaat ttgtcaatcc cttgtcagaa accaggcctt
     1261 gataaacctt gtcagaaaaa ataggaaatt tagaaaaatg ctcttgctcc acaatttcta
     1321 aagcctttat caatttgtcc ttgccctcaa aaataaccgg cttaattttc tgggtctttt
     1381 gcaaatagtc ggcaatagta atcggttctt ggtactgggc tatcactttt tccaaaacag
     1441 tgatcaattc ctgcgaaggt tgggctactt ccttcaaacc tgttcgtttc tcatgaacca
     1501 tgagattacg caactgacga gccacataga gtttatccca gttcgcctca acgggattat
     1561 gtcgcgtcaa ttcctttgcc ttggtcaaca tggaaccaac gtccgcatat tccccatccg
     1621 caacaccgca caccttacgc aagaccttat ccaagtcatt gaataactct aaaaaacggg
     1681 tttcattcat cttaatctac cattgctgat ttcaaatagg catctaccat ccgctcagtc
     1741 gcaactacag aatccaaatg ggtccgctcg taggaatggc tagactcaat gccagcacca
     1801 agcagtgcat gcttgacatc tgcacctgct cgcatagctg ccgaagcatc ggaaccatag
     1861 taaggataaa tatccagctt ataaggaatt ccatctcttt cagccaaggc taccaagtgc
     1921 tgacgaagtt cataatggta gggacctgag ccgtccttga cacagataga aactgtatat
     1981 tcgtcagttt gctggtcatc tcccattgcc cccatatcga ctgccaaata ttcaaccacc
     2041 tgagcaggaa tgctggagtt ggcaccgtag ccaatctctt cgttgtttga aaaatagaaa
     2101 tgtgtcgtat aaggcagggt cactccttct tccttataga ccttcaaaag atgaagcaag
     2161 atagctgcgg ataccttatc atccaagtga cgactcttga taaagccagt ttctgtcacg
     2221 acggtacgag gatcaaagga gataaaatct ccgacttcaa tacccaaggt ccgcgtctcg
     2281 tccgcattgg tcactttttc gtccaaacga atttccatgt tggtctgatt gcgctcagcg
     2341 gtactggcat cccgatagac atggacagag gtctggtgca tgagaatagt acctgtgaaa
     2401 gtcttgccat ttttagcgca atgaattaag cagttttctc cctcaataga agggtatcca
     2461 aatcctccca ccaaatccat tttaagtcgg ccatctggtt tcactgcacg aaccatggcc
     2521 cccagtgtgt ctaggtgagc agtcaccatg cggtgttcct ggtcattttc ccccggaact
     2581 gtcaccaaaa caccaccttt agcggtctta ctagcagcat agccgaaact ttcaacctct
     2641 gccttgatgt aattcataat atccgtcgta aaaccagttg gcgacggagt gttggtcaat
     2701 gtaacaatgt aatcaagtgt cttcatgaaa acctccttat ttgaaactat tatatcacgt
     2761 tttaggtgaa ttttttgcta taatgaaggt atgaaaacct atcagaaaat atatctcctt
     2821 ttaaaagaga gagaggatta tgtcagcgga gaagatttgg cacaggaatt gggcatttca
     2881 cggacatcca tttggaaggc cattcgccag ttagaaacgc atggtttaac tattgaggct
     2941 gcccgtaatc gtggctacag attagccgaa ggcgatttac tcctacccga attaatctct
     3001 caggagcttc aactacctgt ccatctgaat atggatagcg attccaccca actagatgcc
     3061 aaacaaggta ttgaatcagg tcatactagt cctgccctat atttagctcc ctatcagaat
     3121 aaagccaagg ggcgatttgg gagacctttt tatgcatcca agtcaggtgg catttatatg
     3181 tcgcttcgtc tctctcccaa tgttcccttt ctagaattta agccctacac cattttagct
     3241 gcagctgctg ttgtgaaggc cattcaatct ctttgcgact tggacgtcca aatcaagtgg
     3301 gtcaatgaca tctatctcgg acacaaaaag gtcgctggca tcctcactga ggctatttct
     3361 tctatggaaa accaacgggt aaccgatgtc attatcggtg tcggcatcaa tgtccgcata
     3421 gacgatttcc ctaaggaatt acaacaatct gctggaaacc ttttcgagga acaaccacca
     3481 tttacccgca atcaactcat tactgccatc tggaaagcct tcttagaaac cgatgagaag
     3541 gaactaattg ccctttacaa agaaaaatca cttgtcgttg gccaacaagt gagctttgta
     3601 gaaaatcaag ttgaatttaa ggggactgcc atcgctgtta ctgatacagg aaacctggtt
     3661 atccagttag ataacggcaa agcaaaaatt atctccagcg gagaaatcag ccttacttct
     3721 tggtctgctt ctccaccaaa cgaataaatt tagtcacctt gtaagcaaaa tgaagattcc
     3781 gataggcgat aaaggcgtag atacctgcaa tcaagaaatc tcctgtcaaa atcgatgcat
     3841 tcataaaata aacagctaaa atagttgtaa tggctaaaaa aagaaattgt ggaagtttca
     3901 taaaatagat tataccaaaa attcagtcaa ctggctgaat ttttatatgc tttcttgata
     3961 ggtttcgatt cttttctaaa caagaaaaca ccaatccaat tggtgcttt
//
LOCUS       DBNFEG010000170         3995 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.170, whole genome shotgun sequence.
ACCESSION   DBNFEG010000170 DBNFEG010000000
VERSION     DBNFEG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.170"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..3797
                     /locus_tag="AC1PNG_001850"
     CDS             <1..3797
                     /locus_tag="AC1PNG_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HIP6702331.1"
                     /translation="NKKSFDWYGTKQQFSIRKYHFGAASVLLGVSLVLGAGTQVVKAE
                     EAVTSSEPTIASSVAPASTEAVAEEVDKTNAEATLVPAENTSAVATTSTEVEKAKAVL
                     EQVTSESPLLAGLGQKELAKTEDATLAKAIEDAQTKLAAAKALLADSEATVEQVEAQV
                     AAVKAANEALGNELQKYTVDGLLTAALDTVAPDTTASTLKVGDGEGTLLDRTTTATPS
                     MSEPNGAAIAPHTLRDKVEATKESGWYTFESYDLNSYNRNQNGSIVGGTAVGAYIRYS
                     LDSDPATSTVLAELVSTKDGEVLESHKLEAGNSVTFSYPKTINAKNSNITLTYDTSTA
                     TADIPGSLKFYDDRDAVYSTVVVPAYQVNTTRYVTEDGTVLATYSLQTIAGQTVTSSK
                     VRTFTGYDYVKTTQNAIQGAYPKGTLMLAGVGADKNGNKYYKAIREVVEDNQSVMTLY
                     LLDPTYTGTVDWTGTDTTGFIPLLKTSPTVYTIDRKVYDYNINATILSPYTVDNGFMV
                     FKESATNAQGSKYRVVAQWSGTDETDPTKPEYTGGKYGRIYIGTQVWTTAKELGITEN
                     QWRGTADKFDNNGFVPFGTQQYLRNASVATAVETTHVYKVNPKFGDVIVEYYDTDGKQ
                     IVNSVVDTPKSALGTEYNTDVDRRPASLVAADGTVYFYKEVKSDSAKTTGTVVAGTTT
                     VKYVYEKAGSVNVNFVDINGKVIKAPVSDEKDAKPGYNYDTDLDQKLASITFEGKEYK
                     LVPAGDYPVGKVGKGNNLIEVGNNTAKGIDPTTGKIEAGVNKEVTYVYKAVTGSVVVN
                     YKDTEGNVIKDPETDVSDAPVGDAYTTTDKKPNEIITKDGSRYVLVPSKTDGEENGKV
                     IEGTITVTYVYQKVANWIPEIPNVPETNRPKVPYPFDPTEPDEPIDPTTPGTNGEVPN
                     IPYVPGYTPVDPKDNTPLKPIDPNDPGKGYVPPTPENPGVDTPIPYVPVKKVVTNHVD
                     EEGNPIAPQEEGTKPNKSIPGYEFTGKTVTDEDGNTTHIYKKTPEVKNGTVVVNYVTE
                     DGTVIKEPVTDTPTSPEGTPYDTTDNKPKTITFKGEEYELVRVDGTENGKVVEGETVV
                     TYVYRKVETPAKKVVTNHVDEEGNPVAPQEEGTKPNKSIPGYEFTGKTVTDEDGNTTH
                     IYKKTPAKKVVTNHVDEEGNPIAPQEDGTTPKRQISGYEYVRTVVDEEGNTTHIYRKL
                     SNKPTTPEKETPAKPQAGKTASGKAQLPNTGEASSVAGALGTAMLVATLAFARKRRRN
                     ED"
BASE COUNT         1344 a          776 c          859 g         1016 t
ORIGIN      
        1 caaataaaaa atcatttgac tggtacggca cgaaacaaca attttcgatt cgtaagtatc
       61 attttggtgc agcaagtgtt ttgcttggtg tgtcgttagt tttaggtgcc ggtacacagg
      121 ttgttaaagc tgaggaagct gttacttcat cagaaccaac tattgccagt agtgtagcgc
      181 ctgcttcaac agaagctgtt gcagaagaag tagataaaac aaatgctgaa gctacgctag
      241 taccagctga aaatacgagt gcagtagcta cgacttcaac agaagttgaa aaagcgaaag
      301 ctgttcttga acaggtaaca tcagaatcac cacttttggc tggtcttggt caaaaagagt
      361 tggctaaaac tgaagatgca actcttgcaa aagctataga ggatgctcaa acaaaacttg
      421 cagcagctaa ggcacttttg gctgactcag aagcaactgt tgagcaagtt gaagcgcaag
      481 tcgcagcggt taaagcagcc aacgaggcgc tagggaatga attgcaaaaa tacactgtag
      541 atggtctctt aacagcagca cttgatacag tagcacctga tacaactgca tcaacattga
      601 aagttggtga tggcgaaggt acccttctag atagaacgac aacagcaaca ccttcaatgt
      661 ctgaaccaaa tggtgcagca attgctcctc atacacttcg agataaagtt gaggctacaa
      721 aagaatctgg ttggtatact tttgaatcat atgatttaaa ttcctacaat cggaaccaga
      781 atggttcaat tgttggtgga actgctgtcg gcgcttacat acgttattcc ctggactcgg
      841 atccagctac atcaactgta ctagcggagc tagtgagtac taaagatgga gaagtcttag
      901 aaagccataa acttgaggct gggaatagtg tgacttttag ttatccgaag acgattaatg
      961 cgaagaatag caacattact ttgacttatg atacttcaac agctaccgct gatattccag
     1021 gttcgttgaa attttatgat gatagagatg cagtatattc tacagttgta gtgcctgcat
     1081 atcaagttaa tacgactcgc tatgttactg aagatggcac agttttggcg acttacagtc
     1141 ttcaaactat tgcaggtcag acggttactt catctaaagt acgtactttt actggttatg
     1201 actatgtgaa aacaacgcaa aatgctattc aaggtgctta tcctaaaggc acacttatgc
     1261 tagctggtgt cggtgctgac aaaaatggta ataaatatta taaagctatt cgtgaggttg
     1321 ttgaagataa tcaatccgtt atgacattgt acttattgga tccaacttat acgggtaccg
     1381 tagattggac tgggactgat acgacgggat tcataccgct gctaaagaca tctccaacag
     1441 tttatactat tgatcgaaaa gtatatgatt acaatatcaa cgcaactatt ttatctcctt
     1501 acacagttga taatggtttc atggtcttca aggaaagtgc taccaatgct caaggtagta
     1561 aatatcgtgt cgttgcacag tggtcaggca cagatgaaac tgacccaact aaaccagagt
     1621 ataccggagg aaagtatgga cgtatctata ttggtactca ggtttggaca actgcaaaag
     1681 aattgggaat taccgaaaac caatggagag gtacagcgga taagtttgat aataatggtt
     1741 ttgttccatt tggtacccaa caatatctcc gaaacgcttc agtggctaca gctgtagaga
     1801 caacccatgt ctacaaagta aatcctaaat tcggtgatgt cattgttgag tactacgata
     1861 ctgacggaaa acaaattgta aattcagttg tagatactcc taagtcagct cttggcacag
     1921 agtataatac agatgtggac cgtagaccag ccagcttggt tgctgctgat gggacagtct
     1981 acttctacaa agaagttaag tctgattcag ctaagacaac cggtacagta gttgcaggta
     2041 cgacaactgt taagtatgtt tacgaaaaag ctggtagcgt taatgttaac ttcgttgaca
     2101 tcaatggtaa agtaatcaaa gctcctgttt cagatgaaaa agatgcgaaa cctggttaca
     2161 attatgatac cgacttggat cagaaattag cttccatcac ttttgaaggc aaggaataca
     2221 aacttgttcc tgctggtgat tatccggttg gtaaagttgg caagggaaat aacttgattg
     2281 aagttggtaa taatactgcg aaaggtattg acccaacaac aggcaagatt gaggctggtg
     2341 ttaacaaaga agttacctat gtctataaag cagtgacagg ttctgtagtt gtaaattaca
     2401 aagatacaga aggtaatgtg attaaagatc cagaaacgga tgtgtctgat gcaccggttg
     2461 gagatgctta tactacaact gacaagaaac caaacgaaat catcacaaaa gatggatcac
     2521 gctatgtcct tgttccatct aagacagatg gtgaggaaaa tggtaaagtt atcgaaggaa
     2581 caatcacagt aacttatgtt tatcagaaag ttgcaaactg gattccagag attccaaatg
     2641 taccagaaac aaaccgtcca aaagtacctt acccatttga cccaacagag ccagacgagc
     2701 caatcgatcc aacgacacca ggaacaaatg gcgaggttcc aaatattcct tacgttccag
     2761 gatatacacc ggttgatcct aaggataaca cgccgttgaa accaattgat ccaaatgatc
     2821 caggtaaggg ttatgtacca ccaacaccag aaaatccagg tgttgataca ccaattcctt
     2881 atgttccagt taaaaaagtc gtaactaacc acgttgatga agaaggtaat cctattgcac
     2941 cgcaagaaga gggaacaaaa ccaaacaaat caatcccagg ttacgagttc acaggtaaaa
     3001 ctgttactga cgaagatggc aacacaactc acatctacaa gaaaacacca gaagttaaga
     3061 atggtacagt tgttgttaac tatgtaacag aagatggcac agttatcaag gaacctgtaa
     3121 cagatacacc aacttctcca gaaggcacac catacgacac tacagacaac aaacctaaga
     3181 caatcacttt caaaggtgaa gagtatgaat tggttcgtgt tgacggtaca gaaaatggta
     3241 aagttgtaga aggtgaaaca gttgtgactt acgtttaccg taaagtcgaa acacctgcta
     3301 agaaagttgt aactaaccac gttgatgaag agggtaaccc tgttgcgccg caagaagagg
     3361 gaacaaaacc aaacaaatca atcccaggtt acgaatttac aggtaaaact gttactgacg
     3421 aagatggcaa cacaactcac atctacaaga aaacacctgc taagaaagtt gtgactaacc
     3481 acgttgatga agaaggtaac cctattgctc cacaagagga tgggacaaca ccaaaacgtc
     3541 aaatttcagg ttacgagtat gtgcgtactg tagttgatga agaaggtaac acgacacata
     3601 tttatcgcaa actttctaat aaaccaacaa cacctgagaa ggaaactcct gcaaaacctc
     3661 aagcaggtaa aaccgcttca ggtaaagctc aattgccaaa tactggtgag gcttcatctg
     3721 tggcaggtgc gcttggtaca gcaatgcttg tcgcaacact tgcgtttgca agaaaacgtc
     3781 gtcgtaacga agattagtca aaattcttta tacagacttt attcccccat atagaaagta
     3841 taagaattgt acgtaacatg caggattgcc tttccgaaaa aatgaggctg tgcaaaaagt
     3901 ccagagttac atcttagagt tcgctccatt tccaacctcc aacagtcact actctgactg
     3961 ttggagctgt gtgggggtgg gagacgagct ctttt
//
LOCUS       DBNFEG010000171         3952 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.171, whole genome shotgun sequence.
ACCESSION   DBNFEG010000171 DBNFEG010000000
VERSION     DBNFEG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.171"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..189
                     /locus_tag="AC1PNG_001851"
     CDS             <1..189
                     /locus_tag="AC1PNG_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702332.1"
                     /translation="AMSRRQYWSAAQLIRLDLECKTRTNPPINHCAEMLTRTLRSGAG
                     LFAQPHVKDKQNDYKTEK"
     gene            complement(211..1170)
                     /gene="nrdF"
                     /locus_tag="AC1PNG_001852"
     CDS             complement(211..1170)
                     /gene="nrdF"
                     /locus_tag="AC1PNG_001852"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HIP6702333.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLTAEEKDLVGKVFGGLTLLDTLQSETGVQALRNDIRTPHEEAVYNNIQFMESVHAK
                     SYSSIFSTLNTKSEIEDIFEWTNSNEYLQRKAKIINEIYETGTPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELSEEEQDKLR
                     DWMYDLLYQLYENEEGYTRSLYDAVGWTEEVLTFLRYNANKALMNLGQDPLFPDSADD
                     VNPIIMNGISTGTSNHDFFSQVGNGYLLGEVEAMTDDDYLYGL"
     gene            complement(1300..3459)
                     /gene="nrdE"
                     /locus_tag="AC1PNG_001853"
     CDS             complement(1300..3459)
                     /gene="nrdE"
                     /locus_tag="AC1PNG_001853"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HIP6702334.1"
                     /translation="MSLKELGDVSYFRLNNEINRPVNGQIPLHKDQEAVKAFFKENVL
                     PNTKQFDSILDKIAYLLEENYLEKEFLDQYSPEFIIKIDQFLKDQNFRFKSFMAAYKF
                     YNQYALKTNDGAYYLESMEDRVLFNALYFAEGNEELALNLANEMIHLRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGISLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLDVFHPDIISFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARKNEDMYLFSPYSVELEYGVPYSYLDITEKYDELVANPRI
                     RKTKIKARDLETEISKLQQESGYPYVINIDTANRSNPVDGKIIMSNLCSEILQVQTPS
                     VINDSQEYLTMGTDVSCNLGSTNIVNLMKSPDFGRSVRTMTRALTYVTDHSHISAVPS
                     IEAGNSQAHSIGLGAMGLHSYLAQNLIDYGSKTAVEFTNIYFMLLNYWTLVESNNIAR
                     ERKATFHNFEKSKYADGTYFDKYVTGDYQPQSERVKELFEGIFIPSGQDWADLREKVK
                     ADGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLATETI
                     PFYKSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLRSELPKELYEWKKENKQTTRDLS
                     ILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI"
     gene            complement(3492..3710)
                     /gene="nrdH"
                     /locus_tag="AC1PNG_001854"
     CDS             complement(3492..3710)
                     /gene="nrdH"
                     /locus_tag="AC1PNG_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HIP6702335.1"
                     /translation="MVTIYSKNDCVQCKMTKKFLDQHSVDYKEINLDEQPEFIDHVKV
                     LGFSAAPVIETENDVFSGFQPGKLKALV"
BASE COUNT         1102 a          761 c          890 g         1199 t
ORIGIN      
        1 gcaatgagcc gcagacagta ctggagtgcc gcacagttga ttaggttgga tttagagtgt
       61 aaaacacgaa caaatccgcc aatcaaccac tgcgctgaga tgttgacacg aactctaaga
      121 agtggcgctg ggctttttgc ccagcctcac gtcaaagata aacaaaatga ctataaaacg
      181 gaaaaataga aatgatattt ttcctaagat ctacaaccca tataaataat catcatctgt
      241 catagcttcc acttcaccaa gaagatagcc gttaccgact tgggagaaga agtcgtggtt
      301 agatgtaccg gttgagatac cgttcatgat gattgggttg acatcatctg ctgaatctgg
      361 gaagagtgga tcctgaccca agttcatgag ggccttgttg gcattgtaac gaaggaaagt
      421 caagacttcc tcagtccagc cgactgcgtc atagagggag cgtgtgtagc cttcttcatt
      481 ttcgtaaagt tggtagagca ggtcgtacat ccagtcgcgg agtttatctt gttcttcctc
      541 ggacaattca ttgaaaccaa gttggaactt gtaaccgatg taggtaccgt gcacagactc
      601 gtcacggatg atcagcttga tgatttctgc aacgttggcc agcttgttgt taccaaggta
      661 gtagagtggc gtgaagaaac cagagtagaa gaggaaggtt tctaggaata cgctagctac
      721 tttcttttca agaggtgtac ctgtttcgta gatttcgttg ataatctttg ccttgcgttg
      781 caaatattcg ttgctgttgg tccattcaaa gatgtcttca atctcagact tggtgttcaa
      841 ggttgagaag atagaagagt aagacttggc atggactgac tccatgaatt ggatgttgtt
      901 gtaaacagct tcttcgtgcg gtgtacgaat atcgttgcgg agggcttgga cacctgtttc
      961 agattgaagg gtatccaaga gggtcaatcc accaaagacc ttaccaacca ggtctttttc
     1021 ttcagcagta agtttacgcc agtcatccaa gtcgtttgat aaaggaatac gtgtatcgag
     1081 ccagaattgc tcggtcaact tttcccaagt agacttgtcg ataacatcct caatggcgtt
     1141 ccagtttatg gctttgtagt aggtttccat catattctct ttctttttct tatacttcaa
     1201 atattatatc acaaatagat agtagcctgt aaaaaaatcc cctaaaaggg attttaatcg
     1261 attgcttatt agttgcttca catcgtgaac gatagtgtct taaatcacac aactttcaca
     1321 ttggtttgcg ccgacttctt cgccgtcatc ggtaaaggta cggatgtagt agatagactt
     1381 gatacctttg ttgaaggcgt agttacgaag gatagagagg tcacgtgtag tttgtttgtt
     1441 ctcttttttc cattcataga gttcttttgg gagctcgctg cgaaggaaaa gagtgagtga
     1501 caacccttgg tcgacgtgtt ctgttgcggc agcatagaca tcgataacct tccgcatatc
     1561 catgtcatag gcagatttgt agaatggaat ggtttctgtt gccaagccag ctgctggata
     1621 gtagatttta ccgattttct tctcttggcg ttcttcaatc cgttgtgtga ttgggtgaat
     1681 tgaagcagaa acgtcgttga tatagctgat agatccattt ggagcaacag ctaagcggtt
     1741 ttgatgatag agaccgtctg cttttacttt ttcgcggagg tcagcccaat cttgtccact
     1801 tggaatgaaa atgccttcaa aaagttcttt aacacgctcg gattgtggtt ggtagtcacc
     1861 tgtcacgtat ttatcgaaat aagtgccgtc tgcatatttt gatttttcaa agttatggaa
     1921 agtagctttg cgttcgcgag caatgttatt tgattctacc aaggtccaat agttaaggag
     1981 catgaagtag atgttggtaa attctacggc agtttttgaa ccgtaatcaa tcaagttctg
     2041 agctaagtaa gaatgaagtc ccatagctcc aagaccgata gaatgtgcct gactgtttcc
     2101 tgcttcaata gatggtacag ctgaaatgtg tgaatgatct gtgacataag tcaaagcacg
     2161 agtcatggta cggacagaac gtccaaagtc aggcgatttc atcaagttga caatgttggt
     2221 tgaaccaagg ttacatgata catctgtccc cattgtcaag tattcctgag agtcattgat
     2281 gacgcttggt gtttgaactt gaaggatttc cgaacagagg tttgacatga taatcttgcc
     2341 gtcaacaggg ttgctacggt tagctgtatc gatgttgata acgtatggat agccagactc
     2401 ttgttggagt ttggaaattt ctgtttccaa gtcacgtgcc ttgatttttg tcttacgaat
     2461 gcgagggttc gccaccaatt catcgtattt ttctgtgatg tcaaggtagc tataagggac
     2521 accgtattcc aactcaactg agtatgggct gaagaggtac atatcttcgt ttttacgtgc
     2581 caattcgtag aatttatcag gaacggtgat accgagtgat aaggtcttca cgcgaacttt
     2641 ctcatcggca ttttctttct tagtcgaaag gaaggaaata atatctgggt ggaaaacatc
     2701 aagataaacg acaccagccc cttggcgttg cccaagctgg ttagaatagg agaagctgtc
     2761 ttcgaagagt ttcataacag gaacaacccc agaagctgcc ccctcatagc ccttgattgg
     2821 agcacctgcc tcacgcaagt tgctaaggga aataccaaca ccaccaccga tacgggacaa
     2881 ttgcagagca gagttgatag aacgtccaat cgagttcata tcgtcagtga cctggatgag
     2941 gaaacaagac accaactcac cacgacgggc acggcctgcg ttgaggaaag acggtgttgc
     3001 aggctggtaa cgaagatgaa tcatttcgtt ggccaggttt agtgctaatt cttcgttgcc
     3061 ttcagcaaaa tacaaggcgt tgaagagaac acggtcttcc atactttcca agtagtaagc
     3121 cccgtcgttg gttttgaggg catactgatt atagaacttg taggctgcca tgaaagactt
     3181 gaagcggaag ttttggtctt tcaagaattg atcaattttg atgataaatt ctgggctata
     3241 ctggtccaaa aattcttttt caagatagtt ttcttctaat agataggcaa tcttgtctaa
     3301 aatactatca aattgcttgg tatttgggag gacattttct ttaaagaagg ccttaaccgc
     3361 ttcttggtcc ttgtggagtg ggatttgacc attaacagga cggttgattt cgttatttaa
     3421 acggaagtag gacacatcgc ctagctcttt taaactcata gtcgcaatct ctttctataa
     3481 acagtaggga gttaaaccaa ggcttttagt tttcctggtt gaaaacctga gaaaacatca
     3541 ttttcagttt cgataacagg tgcagctgag aaaccgagaa cttttacgtg atcgataaat
     3601 tctggctgtt catctagatt gatttcttta taatctacac tatgttggtc caagaatttc
     3661 ttggtcattt tacactgtac acaatcattt tttgaataga tcgttaccat tcaatttctc
     3721 ctttgtccat gagggattaa taaaaaatcc caattttgtt accaatatag gatacaagat
     3781 tttaaggtta aagtcaagaa ggaagtttgt ttaaaatcac aaaatatagt gcacaatatt
     3841 tttataagac accatatatt gtacatctgt ccaatgctgc caagtcgaaa aatagcgttt
     3901 acatagctgt ctctatttag tttgataatc ctagcaatct ctcggtaaag ta
//
LOCUS       DBNFEG010000172         3799 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.172, whole genome shotgun sequence.
ACCESSION   DBNFEG010000172 DBNFEG010000000
VERSION     DBNFEG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.172"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1162
                     /locus_tag="AC1PNG_001855"
     CDS             <1..1162
                     /locus_tag="AC1PNG_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702336.1"
                     /translation="RLQKNDFHTENFQNGAKIGSIIGLNMMLGFHLKDIQFNAIAKAP
                     QGHGHAAEYANNVIDKIKYPFQKVEQVGRDNVKNGADRIVGNQKIQTKYYAKASGTIN
                     AAFDKANGGEYRYKGMQLEVPKDQYNESIQLMKKKIKAGKVPGHSEPDDAYLIIRKGN
                     VTYNEAKLVAKGGNIVSFSQQIGKILTKKIADQTVKNVAANTVARQAAGVISFGITVG
                     PDVFDLLQGRISSQQLLKNSLVAGTGLLGGVAGGAVAGAFLGPVGSFVGAVAGGTLAA
                     VGSKVVLDNYIEDDRIEMFAILKEEFIDIVMAMALSEEEFKEIQNNIFDKKLGERLKD
                     MFQKKQSVKNRMFTRESIIEREVERVISKRTVLAEEDILEAVQIAEKEFAVI"
     gene            1206..2294
                     /locus_tag="AC1PNG_001856"
     CDS             1206..2294
                     /locus_tag="AC1PNG_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HIP6702337.1"
                     /translation="MISGIIAEYNPFHTGHKYLLEQAEGLKIVVMSGNFMQRGEPAIV
                     DKWTRAQMALEHGADLVVEMPFLVSVQSADHFAKGAISILHKLGVEKLVFGTEEMLDY
                     QKIADIYVDKSEEMENFVKNLPDNFSYPQKTQAMWQEFAGLTFTGDTPNHILALAYAK
                     AVAGTGIQLSPVQRQGAGFHSEEVETSYASATAIRKGADQLDLVRGFLPSVSLFEEAT
                     KVSWRNYFPLLRYQIATHPDLSQVFQVNEELASRIRSAIGSVATVEELVDAVATKRYT
                     KARVRRVLTYILINAVETPLPEAVHVLGFSTRGQAYLKQVKERVDLVTRIGKEPWDSL
                     TQQADAVYQLGADAMAEQTYGRVPVRVE"
     gene            2296..2676
                     /locus_tag="AC1PNG_001857"
     CDS             2296..2676
                     /locus_tag="AC1PNG_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TipAS antibiotic-recognition domain-containing
                     protein"
                     /protein_id="HIP6702338.1"
                     /translation="MKKEMSQLEAYKAEAKERWGQTATYAEFEVRYDASKDHLFAQEM
                     QAIFEAFGKMQSLGAVHPDVQAQVANLQAYITENFYTCTKEILQNIGLMYVEDERFSA
                     NIDRAGGLGTAAFVSQAIAIYCQE"
     gene            2736..>3799
                     /locus_tag="AC1PNG_001858"
     CDS             2736..>3799
                     /locus_tag="AC1PNG_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HIP6702339.1"
                     /translation="MKEKICKKLKELAKKPVRRIDWSGERWFVRRYKQGLALAWIVYF
                     LLREELVKEILKPVSNYLMNFSCIQWFYVNKLTVLLVFTALLFLAKSIFSILRWCEVI
                     RYFPLGKKKYPEYDFPFQEELVSFLNRKEERGRNIFWLDGSWGSGKTHFIRTFFENQL
                     YKPREIYYISCFGIRTREQAEKALIEEIEQHSTFGSLDHIPVVSSLVKWSYKILGLDL
                     MKKYSLIIFDDLERVAYSEKQKDKPEDFNDLLGFIDHLANHRNQKVLVLMNKEDMGNT
                     YQKIVEEKFKPVVTNIPSQKDIIKNMTEEYDDMSREFIRNIYLFQHEFGFINLRAIPF
                     MYSVFESVRQNGVYSVEWFVT"
BASE COUNT         1171 a          644 c          954 g         1030 t
ORIGIN      
        1 tcgtttgcaa aaaaatgatt ttcatacaga gaattttcaa aatggagcta aaataggatc
       61 aattataggt ttaaatatga tgctaggttt tcatttgaaa gatattcaat ttaatgctat
      121 tgcaaaagct cctcaaggac atgggcatgc agctgaatat gctaataatg taatagacaa
      181 gataaagtat cctttccaaa aagtagagca agtagggaga gataatgtta aaaatggggc
      241 agatagaatt gttggaaatc aaaaaattca aacaaaatac tatgcaaaag ctagtggaac
      301 gataaatgca gcatttgata aggctaatgg aggagaatac cgttataagg ggatgcagtt
      361 agaagttcct aaagaccagt ataatgaatc aattcaactc atgaagaaaa aaataaaagc
      421 tggaaaagtt ccgggtcata gtgagcctga tgatgcctat ctgattatta gaaaagggaa
      481 tgttacttat aatgaggcaa aactggttgc caagggcgga aatattgttt cattttctca
      541 acaaattgga aaaattctta ctaaaaaaat cgctgatcaa acagttaaga atgtcgcagc
      601 caatactgtt gctagacaag cagcaggagt tatttcattc ggaattactg taggtccgga
      661 cgtatttgat ctactgcaag gtagaatatc tagccaacaa ttattaaaaa atagcctagt
      721 agcaggaaca ggcttactag gtggagttgc aggtggtgcg gttgctggag catttttagg
      781 tccagttgga tcatttgtag gtgcagtagc tggtggaaca ttggctgctg ttggttctaa
      841 ggttgtttta gacaactata tagaagatga cagaattgag atgtttgcaa ttctaaaaga
      901 agaatttatt gatattgtta tggctatggc gctttcagaa gaagagttta aagaaattca
      961 aaataatatc ttcgataaga aattaggaga acgacttaaa gacatgttcc agaaaaagca
     1021 atctgttaaa aataggatgt ttactagaga gtcaataatt gaacgagagg ttgaaagagt
     1081 tatctcaaaa cgcacggttt tagcagaaga ggatatactt gaagcagtac agattgcaga
     1141 aaaagagttt gctgtaattt aggtattagg ttgtagatgg ttgaataaaa tctaggaggt
     1201 agggcatgat ttcagggata atagcagaat acaatccctt tcacacgggg cataaatacc
     1261 tgctggaaca ggccgagggg ttgaaaattg tggttatgtc aggcaacttc atgcaacggg
     1321 gtgagccagc cattgtggat aagtggacac gggctcagat ggctttggaa cacggggcag
     1381 atcttgttgt cgagatgccc tttttggtgt cggtccagtc ggctgaccat tttgccaaag
     1441 gagcaatcag cattttacac aagctaggtg tggaaaaact ggttttcggt acggaagaaa
     1501 tgctggatta ccagaaaatt gcggatatct atgtggataa gtcggaagag atggaaaatt
     1561 ttgtgaaaaa cttgccagat aatttctctt atccacagaa gacccaggct atgtggcagg
     1621 agtttgctgg gctgactttc acaggtgaca cgcccaacca tattctggct ctagcttacg
     1681 ctaaggcagt ggctggaaca ggcattcagc tcagccctgt tcaacggcag ggagctggtt
     1741 ttcattcgga agaggtggaa acgtcctacg cctcggcgac agctatccgc aagggtgctg
     1801 accagctgga cttggttcgc ggctttttac cgtctgtcag tcttttcgaa gaggcgacca
     1861 aggtaagctg gagaaactat tttccgctgc tccgctacca gattgcgacc cacccagatc
     1921 tcagtcaggt ctttcaggtc aacgaagaac tagccagtcg tatccgctct gccattggga
     1981 gcgtagcgac cgtggaggag ttagtggatg ccgttgcgac caagcgctac accaaggcgc
     2041 gggtgcggcg ggtgctgacc tacatcctga tcaatgcggt ggaaactccc ttgccagaag
     2101 cggtccatgt cctaggcttt tcaactcgag gccaggccta tctcaagcag gtcaaggagc
     2161 gggtggacct ggtgacgcgg atcggcaaag agccctggga cagcctgacc cagcaggcgg
     2221 atgcggtcta tcaactgggc gctgacgcaa tggcagagca gacctatggg cgcgtgccgg
     2281 tgagggtgga gtgagatgaa gaaagagatg agtcaactag aagcctacaa ggcggaagca
     2341 aaggaacgct ggggacagac cgcgacctat gccgagtttg aagtacgcta tgatgctagc
     2401 aaggaccact tgtttgccca agagatgcag gctatttttg aagcttttgg aaaaatgcag
     2461 agcttgggag ccgttcatcc agatgtgcag gctcaggtag cgaacttgca ggcctatatc
     2521 acggaaaatt tctacacctg cacaaaggaa atcttgcaaa atataggtct tatgtatgtt
     2581 gaggacgagc gattttcagc caacattgac cgagcaggtg gactaggaac agcagccttt
     2641 gtcagtcaag ctattgcgat ttattgccaa gaataaacga aagaagtctg gtgagaacta
     2701 ggcttttttt ggtatactaa actgaggagg aagttatgaa agaaaaaatt tgtaagaagt
     2761 taaaagaact cgccaaaaag cctgtacgac gaatagattg gagcggagag agatggtttg
     2821 tccgaagata caagcaaggt ttagctttgg cgtggattgt ctattttctt ttgcgagaag
     2881 agttggtgaa ggaaattctt aaacctgtta gtaactattt aatgaatttt tcctgtatac
     2941 agtggtttta tgtaaataag ctgactgttt tgttagtatt taccgctctg cttttccttg
     3001 cgaagagtat cttttccatc ttgcgatggt gcgaggttat ccgctatttt ccactgggta
     3061 agaaaaaata ccctgaatat gacttccctt ttcaagagga actggtttct ttcttgaatc
     3121 gaaaggaaga acgaggtagg aatatctttt ggttagatgg ttcgtggggg agtgggaaaa
     3181 cccactttat tcgcacattc tttgaaaatc aactttacaa accgcgtgag atttattata
     3241 tctcctgttt tggcattcgg accagggaac aggcggagaa ggcattgatt gaagagattg
     3301 agcaacattc gacctttgga agtttagacc atattcccgt tgttagtagt ttagtcaaat
     3361 ggtcttataa gattttgggc ttagacttga tgaaaaaata ttctcttatt atctttgatg
     3421 atttggaacg tgtagcttat agtgaaaagc aaaaagataa acctgaagac tttaacgatc
     3481 ttcttggttt catagaccac cttgccaacc accgtaatca aaaagtactt gtcttgatga
     3541 ataaggagga tatggggaat acttatcaaa agattgttga agagaaattt aagccggttg
     3601 tgacaaatat tccaagtcag aaagacatca taaaaaatat gacagaggag tatgacgata
     3661 tgagtcggga atttataaga aatatctatc tctttcaaca tgaatttggt tttataaatt
     3721 taagagccat tccttttatg tatagtgtat ttgaaagtgt tcggcaaaac ggagtgtact
     3781 cagtagagtg gtttgttac
//
LOCUS       DBNFEG010000173         3751 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.173, whole genome shotgun sequence.
ACCESSION   DBNFEG010000173 DBNFEG010000000
VERSION     DBNFEG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.173"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..450
                     /locus_tag="AC1PNG_001859"
     CDS             <1..450
                     /locus_tag="AC1PNG_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702340.1"
                     /translation="SPIFNTSEYGDQLQDIWIDSLNKDELEEEFGSYKVYYDGNLIIE
                     NGASYTSEGATKNQIISMKETIVTELDTNKEFKKYTFKKYYIPDEGTDPTEIKIIEDG
                     FAIETVFEQKDLIPNIAFGPTLYTVRAEFIKENNKLVLRKGSMEVYK"
     gene            447..878
                     /locus_tag="AC1PNG_001860"
     CDS             447..878
                     /locus_tag="AC1PNG_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702341.1"
                     /translation="MKSLTQTFTKKQKLVVVIVVILSVAIGFWKWRDTFFNTYYPDDT
                     SIQGQLFPNVANTKIETVENVLPFSDVKYDKIIIRIGSLSSEYDDSNQSSKANNIINR
                     KTLDENFIQWLIEAFDEEEAKKISDHIEVYYRGEIISEISY"
     gene            1054..3705
                     /locus_tag="AC1PNG_001861"
     CDS             1054..3705
                     /locus_tag="AC1PNG_001861"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIP6702342.1"
                     /translation="MSKELSPKYNPAEVEAGRYQTWLDQDVFKPSGDAEAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQG
                     VTRYDLGREKFLDKVWEWKDEYAATIREQWGKLGLSLDYQRDRFTLDEGLSKAVRKVF
                     VELYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALQVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIVNKPIPIVADEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAGEFAGMDRFEARKATVAKLQELGALV
                     EIENRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTADKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNESGEMYVGEEAPAGDGWVQDEDVLDTWFSS
                     ALWPFSTMGWPDENAADFQRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGSQPFKNVL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDAARENVAKVAAGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEAEKVMTRSVLLYTLDQI
                     LRLLHPIMPFVTEEIFAQYAEGSIVVAAYPVVNPAFENAEAHKGVESLKDLIRSVRNS
                     RAEVNVAPSKPITILIKTADSELETFFKANENYIRRFTNPEQLEISSSIAAPELAMSA
                     VITGAEIFLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFIANAKPEVVEK
                     EKEKQADYQAKYDATVGRIAEMKKLVK"
BASE COUNT         1138 a          787 c          880 g          946 t
ORIGIN      
        1 agtcctattt ttaatacttc agaatatggg gaccaattac aagatatttg gatagactct
       61 ttaaataagg atgaacttga agaggaattc ggttcctaca aagtttacta tgatggtaat
      121 ttgattattg aaaatggtgc atcctataca agcgagggtg ctactaaaaa tcaaataata
      181 tctatgaaag aaacgattgt cactgaatta gatacaaaca aggaattcaa aaaatacaca
      241 tttaaaaaat attatatccc agacgagggt acagatccaa cagaaattaa gattatagaa
      301 gatggatttg ctattgaaac ggtgtttgaa caaaaagatt tgatccctaa tattgctttt
      361 ggtcctactc tgtacacagt gagagcggag tttataaagg aaaacaataa actggtattg
      421 aggaagggga gcatggaggt atacaaatga aaagtttgac acaaaccttc accaagaaac
      481 aaaaattagt agtggtgatt gtagtgatac tatccgtagc aataggtttt tggaaatggc
      541 gtgatacttt tttcaatacg tattatcctg acgatacttc aatccaaggg cagttgtttc
      601 ccaatgttgc aaacacgaag attgaaaccg ttgagaatgt cttgccattt tcagatgtga
      661 aatatgataa gataattatc agaattggta gcctgtcttc agaatacgat gattctaatc
      721 aatcatcgaa agcaaataat atcataaaca gaaaaacatt agacgaaaat ttcatacaat
      781 ggctcataga agcatttgat gaagaggaag ctaaaaaaat atctgatcat atcgaagtgt
      841 actatcgtgg cgaaataata agcgaaatta gttattaacg ttacacaaac ctctctcgcc
      901 cgtagatgca gggttcaaga atgaaaaacg aaatagccag cccaaaggac tgactatttc
      961 taggagatta tatgaaaaag aaaagttttg ctatttctag cttgaggata gtataaacca
     1021 attttcttaa agaatactaa aggagaaaaa ttaatgtcaa aagaattatc accaaaatat
     1081 aatccagctg aggttgaggc tggtcgttac caaacttggt tggaccaaga tgtctttaag
     1141 ccgtcaggcg atgcggaggc taagccgtat tctatcgtca ttccgccacc aaacgtaact
     1201 ggtaagttgc acttgggaca tgcttgggat accacccttc aagatattat tatccgccag
     1261 aaacgtatgc agggctttga caccctctgg ctgccaggta tggaccacgc ggggattgcc
     1321 actcaggcta aggttgagga gcgcttgcgt gagcaaggtg tgactcgtta tgacttgggc
     1381 cgtgagaaat tcctcgacaa agtctgggaa tggaaggatg aatacgcagc gaccattcgt
     1441 gagcaatggg gcaagttggg tctgtctttg gactaccaac gtgaccgctt tacgctggac
     1501 gaaggcttgt caaaagctgt tcgcaaggtc tttgttgaac tctacaaaaa aggctggatc
     1561 taccgtggcg aatttatcat caactgggac cctgcagctc gcacagccct ttcagatata
     1621 gaagttattc acaaggacgt agaaggtgcc ttttaccaca tgaattacat gttggaagac
     1681 ggttctcgtg cccttcaagt tgcgacaact cgtcctgaaa ccatgttcgg tgacgttgcg
     1741 gtggcggtta acccagaaga cccacgttac aaggacttga ttggcaaaaa tgttatccta
     1801 ccaatcgtca acaagccaat cccgatcgtt gcggatgagc acgcggatcc tgaatttggt
     1861 acaggggttg tgaaaatcac acctgcccac gatccaaatg acttcctcgt aggtcaacgc
     1921 cacaatctgc cacaagtcaa tgttatgaac gacgatggta ctatgaacga gttggcaggc
     1981 gaatttgcag gtatggaccg ctttgaagct cgtaaggcaa cggttgctaa gttgcaagaa
     2041 ctgggagccc ttgtggaaat cgaaaaccgt gtgcattctg ttggacactc tgagcgtaca
     2101 ggtgtggtag tcgagccacg tttgtcaact caatggtttg ttaaaatgga ccaattggct
     2161 aagaatgcca ttgccaacca agacacagct gataaggtag aattttaccc accacgtttc
     2221 aacgatactt tccttcaatg gatggaaaat gtacacgact gggtgatttc tcgtcagctt
     2281 tggtggggcc atcagattcc agcatggtat aacgaatctg gcgaaatgta cgtcggagaa
     2341 gaggccccag caggtgacgg ttgggtacag gatgaggatg tcctagacac ctggttcagc
     2401 tctgcccttt ggccattctc aactatgggc tggcctgatg aaaatgcggc ggacttccag
     2461 cgttacttcc caacttcaac cttggtaacg ggctacgaca tcatcttctt ctgggtgtct
     2521 cgtatgattt tccaatcgct tgagttcaca ggcagtcagc cattcaagaa cgtgctgatc
     2581 catggtttga ttcgtgacga agaaggtcgc aagatgtcta aatcactcgg aaacgggatt
     2641 gacccgatgg atgtcatcga gaaatacggt gcggacgctt tgcgttggtt cttgtcaaat
     2701 ggctctgccc ctggtcaaga tgttcgtttc tcttatgaga agatggacgc ttcttggaat
     2761 ttcatcaaca agatttggaa catttcccgc tacatcctca tgaacaatga aggcttgact
     2821 ttggatgcgg cgcgtgagaa tgttgccaaa gtcgcagctg gtgaggctgg caacgtgacg
     2881 gaccgctgga ttctccacaa ccttaacgaa accatcgcta aggtcactga aaacttcgac
     2941 aagtttgagt ttggtgtggc tggccacatc ctctacaact tcatctggga agagttcgcc
     3001 aactggtatg tggagctgac caaggaggtg ctttacagcg acaacgaggc tgagaaagtc
     3061 atgacccgat ctgtccttct ctacacgctg gaccaaatcc ttcgcctcct ccaccctatc
     3121 atgccgtttg tgacggaaga gatcttcgct cagtacgcag agggctctat cgtggtggca
     3181 gcttaccctg ttgtcaaccc agcctttgaa aacgctgaag ctcacaaggg agtggaaagc
     3241 cttaaggatc tgattcgttc ggtgcgaaat agcagagcag aggtcaacgt tgctccttct
     3301 aagccgatta ccatcttgat taagacggca gatagcgagc ttgaaacctt cttcaaggca
     3361 aatgaaaact acatccgacg ctttacaaac ccagagcagt tggaaatcag ctcaagcatt
     3421 gccgcaccag aactagccat gtcagccgtt atcaccggtg ctgaaatctt cttgccactc
     3481 gccgacctcc tcaacgtcga agaagagttg gctcgtctag acaaagaact tgccaagtgg
     3541 caaaaagaac tagacatggt cggcaaaaaa ctcagcaacg aacgcttcat cgccaacgcc
     3601 aaaccagaag tcgttgaaaa agaaaaagaa aaacaagccg actaccaagc taagtatgat
     3661 gcgacagtag gacgtatcgc agaaatgaaa aaattggtta agtaggtaga atacgataaa
     3721 aagcccaacc taaaatggct gggttttgtg g
//
LOCUS       DBNFEG010000174         3749 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.174, whole genome shotgun sequence.
ACCESSION   DBNFEG010000174 DBNFEG010000000
VERSION     DBNFEG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.174"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(102..1475)
                     /gene="dnaA"
                     /locus_tag="AC1PNG_001862"
     CDS             complement(102..1475)
                     /gene="dnaA"
                     /locus_tag="AC1PNG_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIP6702343.1"
                     /translation="MNQEQLFWQRFIELAKVNFKPSIYDFYVADAKLLGINQQVANIF
                     LNRPFKKDFWEKNFEELMIAASFEIYGEPLAIQYQFTEDEQEIRNTTNTRSSIVHQVQ
                     TLEPATPQETFKPVHSGIKSQYTFANFVQGDNNHWAKAAALAVSDNLGELYNPLFIFG
                     GPGLGKTHILNAIGNKVLADNPQARIKYVSSETFINEFLEHLRLNDMESFKKTYRNLD
                     LLLIDDIQSLRNKATTQEEFFHTFNALHEKNKQIVLTSDRNPDHLDNLEERLVTRFKW
                     GLTSEITPPDFETRIAILRNKCENLPYNFTNETLSYLAGQFDSNVRDLEGALKDIHLI
                     ATMRQLSEISVEVAAEAIRSRKQTNPQNMVIPIEKIQTEVGNFYGVSLKELKGSKRVQ
                     HIVHARQVAMFLAREMTDNSLPKIGKEFGNRDHTTVMHAYNKIKTLLLDDENLEIEIT
                     SIKNKLR"
     gene            complement(1678..2442)
                     /locus_tag="AC1PNG_001863"
     CDS             complement(1678..2442)
                     /locus_tag="AC1PNG_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIP6702344.1"
                     /translation="MEELRTLNISEIHPNPYQPRIHFDEKELLELAQSIKENGLIQPI
                     IVRKSSIIGYELLAGERRLRASQLAGLTTIPAVVKELTDDDLLYQAIIENLQRSNLNP
                     IEEAASYQKLISRGLTHDEVAQIMGKSRPYISNLLRLLNLSSQTKQAVEEGKISQGHA
                     RQLVSFSEEKQAEWVQLILSKDLSVRTLEKLIAANKKKHTKLKQRDQFLKEQEDSLSK
                     TLGTATKIIKKKNGSGEIRINFNDLDEFERIINNFK"
     gene            complement(2503..3699)
                     /locus_tag="AC1PNG_001864"
     CDS             complement(2503..3699)
                     /locus_tag="AC1PNG_001864"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIP6702345.1"
                     /translation="MERIPYMKKYLKFAILFVIGFFGGLIGALSASFFQPRVQQANSA
                     ITSVSNVQYNNETSTTKAVEKVQNAVVSVINYQKSANNSLGAIFGNIESSDELAVAGE
                     GSGVIYKKDGQYAYIVTNTHVINNAEKIDILLASGEKISGELVGSDTYSDIAVIKISA
                     DKVTAVAEFADSDTIKVGETAIAIGSPLGSVYANTVTQGIISSLSRTVTSQSKDGQTI
                     STNAIQTDTAINPGNSGGPLINTQGQVIGITSSKITSSSANSSGVAVEGLGFAIPAND
                     AVAIINQLEKTGQVSRPALGVHMVNLTTLSTSQLEKAGLSNTELTSGVVIVSTQSGLP
                     ADGKLETFDVITEIDGEAIQNKSDLQSALYKHQIGDTITVTYYRNNQKQTVDIKLTHS
                     TEELSE"
BASE COUNT         1090 a          678 c          673 g         1308 t
ORIGIN      
        1 gtattctgtg gaaaagtgat gaaagaccaa taaaactgcg gaaattaact tgttcacaac
       61 ttgtggaaaa aacagagttt tttaaaaaag ttatacacag gttaacgaag tttatttttt
      121 atactggtaa tctctatttc taaattctca tcatccaaga ggagagtttt tattttattg
      181 tatgcatgca taacggttgt atggtctcga ttaccaaatt ctttcccaat ttttggaagg
      241 gaattgtctg tcatttcacg tgctaaaaac atagcaactt gtcgcgcgtg aacgatatgt
      301 tgaacacgct tagaaccttt taattctttc aagctgacac cgtagaaatt tcccacttcg
      361 gtttggattt tctcaatagg aataaccata ttttgtggat ttgtttgttt tcttgatcga
      421 atagcctcag cagcaacctc gacacttatc tcagacagtt gacgcatagt ggctatcaaa
      481 tggatatctt ttaaggcacc ttcaaggtca cgtacgttcg aatcaaattg cccagctaga
      541 taggatagcg tctcatttgt aaagttgtaa ggcaggttct cgcacttgtt acgtaaaatt
      601 gcgatacgtg tttcaaaatc aggtggagtg atttcactgg ttaaccccca tttgaaacgt
      661 gttactagtc tttcttccaa attgtctaag tgatcgggat tacggtcgct tgtgagtaca
      721 atctgcttat ttttttcatg aagcgcatta aaagtatgga aaaattcttc ctgtgttgtt
      781 gctttattac ggagagactg aatgtcatca attagaagta agtccagatt gcgataggtt
      841 tttttgaaac tttccatatc attgagacgg aggtgttcta aaaattcatt gatgaatgtt
      901 tccgatgaga catattttat ccttgcctgg ggattatcgg ctagaacctt atttccaatc
      961 gcatttaaaa tatgagtttt tccaagacca ggaccaccaa aaatgaataa tggattgtag
     1021 agctcaccta ggttatcaga tacagctaaa gctgcagcct ttgcccagtg attattgtct
     1081 ccttgtacaa aattagcaaa ggtgtactgg gattttatac cagaatgaac cggtttaaaa
     1141 gtttcttgag gagtagccgg ctcaagtgtc tgtacctggt gaactattga acttcttgtg
     1201 tttgtagtat tcctaatctc ctgttcatcc tctgtaaatt gatattggat ggcaagaggc
     1261 tctccgtaga tttcaaaact agcggcaatc attaactctt cgaagttttt ttcccagaaa
     1321 tcttttttaa atggacgatt taagaaaata ttggcaactt gctggttgat tccgagtaat
     1381 tttgcatcag cgacataaaa atcataaata gatggcttaa aatttacctt tgccaattca
     1441 ataaatcgtt gccaaaaaag ttgttcttgg ttcatatttt ccccctttca cattatattc
     1501 gttagaatag tgtaccataa aaaacaaaag ttttccacag ataattttaa aaatccacac
     1561 tgttaagaaa agttttcaac aagatgtgaa taaaagtcag aacctttgct ggtcctgact
     1621 ttttgtaatg attttagtgt ggaaaagagt gtttataaaa ataaattgta aacaagtcta
     1681 tttaaaattg ttgataattc tttcgaattc atcgaggtca ttaaagttaa tccgaatttc
     1741 tccgctcccg tttttcttct tgataatttt tgtagctgtt ccaagagttt tactgagtga
     1801 atcttcctgt tcttttaaaa attggtcgcg ttgtttaagc ttagtgtgtt ttttcttatt
     1861 tgcagctatt aatttttcaa gcgtacgcac acttaaatcc tttgataaaa tgagttgaac
     1921 ccattcggct tgcttttctt ctgaaaatga caccaattgt cgcgcgtgcc cttgtgaaat
     1981 ttttccttct tctacagctt gtttagtctg agatgataga tttagtaggc gcaataaatt
     2041 actgatatat ggtcttgatt ttcccatgat ttgagcaact tcatcatgtg ttaaccctct
     2101 actaatcaat ttttgataag aggctgcttc ttctatcgga tttaagttag aacgctgcag
     2161 attctctatg atagcctgat agagtaaatc atcatcagtt agttccttta ccactgctgg
     2221 tattgtagtc agtccagcta attgactggc tcgcaacctt ctttctccag ctaataattc
     2281 gtatccgata atagaagatt ttcttacaat aatcggttga attaagccat tttccttaat
     2341 agattgagcg agctcaagta gctccttttc atcaaaatga attcttggct gatagggatt
     2401 gggatggatt tctgaaatat ttagtgtacg taattcttcc atatatcctt attctagcaa
     2461 aaaaaatgag actttacaat tgtgtaaagt ctcatttgtc aattattcgc taagttcttc
     2521 tgtagaatgt gtcaacttaa tgtcaacagt ttgtttctga ttattgcggt aataagttac
     2581 agtgattgta tctccaattt gatgtttgta gagagcgctc tggaggtcac tcttattttg
     2641 aatagcttct ccgtcaatct cagtaataac atcaaaagtt tctaattttc catctgcagg
     2701 tagcccactt tgtgtagaga caattactac accggatgtt aattccgtat ttgataatcc
     2761 agctttttct aattgactag ttgacaaggt cgtcaagtta accatatgaa ctccaagagc
     2821 aggtcggcta acttgtccag ttttttcaag ctgattgata atagctacgg catcatttgc
     2881 aggaatagca aatcccaacc cttctacagc cacgcctgag ctatttgcag aacttgaggt
     2941 aattttgcta gaggtaatgc ctatcacttg tccttgggta ttgattaacg gtccgccaga
     3001 gtttccaggg ttgatagctg tatcagtttg aatagcgtta gttgagattg tttgtccatc
     3061 ttttgattgt gaagtaactg tccgacttaa gctagaaata ataccctggg taactgtatt
     3121 ggcgtagacg ctacctagag gactaccaat tgcgatagca gtttctccaa ctttaattgt
     3181 atcggaatca gcaaattcag caacagcagt gactttatct gctgatattt ttataacagc
     3241 tatatcagaa tatgtatcag aaccaacaag ttcaccgcta attttttctc cagatgctaa
     3301 aaggatatca atcttttctg cgttattaat aacatgcgta tttgtcacaa tataggcata
     3361 ttgaccatct tttttgtaga taacaccaga tccctcccca gcaacggcta gttcgtcaga
     3421 tgattcaata tttccaaaga tagcaccaag actattgttg gctgactttt ggtaattaat
     3481 aacagacaca acagcatttt gtactttctc tacagccttt gtagtagaag tttcattatt
     3541 atattgaaca ttgctgacac tagtgatggc agaatttgct tgttgaaccc gtggctggaa
     3601 gaaagaggct gataaggccc cgataagacc cccaaaaaat ccaattacaa ataaaatcgc
     3661 aaatttcaaa tattttttca tataaggaat cctttccatt tctttaattt tctttaagag
     3721 tactatttct ttcttaatta taacttaaa
//
LOCUS       DBNFEG010000175         3485 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.175, whole genome shotgun sequence.
ACCESSION   DBNFEG010000175 DBNFEG010000000
VERSION     DBNFEG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.175"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            84..524
                     /locus_tag="AC1PNG_001865"
     CDS             84..524
                     /locus_tag="AC1PNG_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702346.1"
                     /translation="MTENDYLEQTEKDRLELEQHRLNYMADDTPIEPSDIPKLMEIAK
                     KLQAEDTSLNIYELYKHPEARAKLFSQITEACYMALNATPTQAQRLAFCDYLEQQYEN
                     TLKKMVASTDKQALGELLDLLELPVEIESQFIRDMAISGLLAKG"
     gene            594..1094
                     /locus_tag="AC1PNG_001866"
     CDS             594..1094
                     /locus_tag="AC1PNG_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702347.1"
                     /translation="MIELTIESITKPLKQKRLSRVSGKLKGVGITIDLNNLTVNYGSQ
                     QINLASIPGTYGGVRYFFLCPICEKRCRKLFKNSLIFACGTCQDRHRATLNRSKTDCQ
                     YYFELAFKEARKIDPSYYPEKGYVDYDNFPSRPKRMRAKTYWKHYRKFTSYLDKGVSN
                     WLGGLK"
     gene            1234..1620
                     /locus_tag="AC1PNG_001867"
     CDS             1234..1620
                     /locus_tag="AC1PNG_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIP6702348.1"
                     /translation="MFEINMTINERLRDIRDLKDAISSLENDKLELEKTYPVQSRRIR
                     KKKARLLVAIRGIKVKRQRMTDLINQLSDENQRKILTLQYIEGVKDKHLVEVSGLKDY
                     REVSSIRQKAIKNLERLQKQLEQPQA"
     gene            1804..1986
                     /locus_tag="AC1PNG_001868"
     CDS             1804..1986
                     /locus_tag="AC1PNG_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002961998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HIP6702349.1"
                     /translation="MPMTQKEMVKLLTAHGWTKTKGGKGSHVKLEKVGERPITIPHGE
                     INKYTERGIKKQAGLL"
     gene            2026..2478
                     /locus_tag="AC1PNG_001869"
     CDS             2026..2478
                     /locus_tag="AC1PNG_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIP6702350.1"
                     /translation="MLVTYPALFYYDDTDGATAPYFVTFPDFEHSATQGEDMADAMAM
                     ASDWLGIHLADYIENGRDIPTPTPINALSLADNNPFRDDKDIELVYDPSKSFVSMVMV
                     DVAEYLGSQEPVKKTLTIPRWADTLGRELGLNFSQTLTDAIADKKIHA"
     gene            2590..2799
                     /locus_tag="AC1PNG_001870"
     CDS             2590..2799
                     /locus_tag="AC1PNG_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BOW99_gp33 family protein"
                     /protein_id="HIP6702351.1"
                     /translation="MAKPKEKWTPIITHIVPKDTELADGTILDKETTLSQEEFSKNPP
                     VIPDGHPFYNIWARIIREKIEKGEL"
BASE COUNT         1216 a          615 c          696 g          958 t
ORIGIN      
        1 gtgttaccaa gattttttaa agttggtaac atttgccgaa attttacata atttttctta
       61 aagtaagaaa tggagactaa aaaatgacag aaaacgacta cctagaacag acggaaaaag
      121 acagattaga acttgagcaa caccgcctaa attatatggc tgatgataca cctattgagc
      181 ctagcgatat tcctaaacta atggaaatag ctaaaaagtt acaagcagag gataccagtc
      241 tgaacattta tgaactgtat aagcacccag aagcgcgtgc taaactattc tcacagataa
      301 ctgaagcttg ttatatggct ttgaatgcta caccgacaca agcccaaaga ttggcatttt
      361 gcgactatct agaacagcaa tacgaaaaca ccttaaagaa aatggttgcc agcacagaca
      421 aacaggcact aggtgagtta ctagatttgt tagagctacc agtagagata gaaagccagt
      481 ttatccgaga tatggcaatc agtgggttac tagccaaagg ctgaacaggg aggcacgcgc
      541 ctcttttgtt gtttaatcaa taaatttggg ttttgccaat aagggagggg aaaatgattg
      601 aattgaccat agaaagcata accaaaccat tgaaacaaaa acgcttatca agagtatccg
      661 gtaagttaaa gggagtaggt atcactattg accttaataa cctaactgtt aactacggta
      721 gccaacagat aaacctagct agcataccag gcacttatgg aggtgtccgc tacttctttc
      781 tttgtcctat ttgtgaaaag aggtgtagaa aactattcaa aaatagcctt atctttgctt
      841 gcggtacttg tcaagatagg catagggcaa ctttgaaccg aagtaaaaca gactgccagt
      901 attattttga actagctttc aaagaggcta ggaagataga ccccagttat tacccagaaa
      961 aagggtacgt tgattatgat aattttccta gcagaccaaa aagaatgagg gctaagacat
     1021 actggaaaca ttacagaaag tttactagct atttagataa aggggtgagc aattggttgg
     1081 gtggactaaa ataacagggt gcatttacaa aacaaacaaa taaagaacgc tctattagtt
     1141 cccaaaacaa ataaacttac tcaatcgaac aaagaacgaa caaagaatac gcgctttttt
     1201 gatataatca tagtcatagc gaggtattaa attatgtttg aaatcaatat gacgattaac
     1261 gagcgactaa gagacattag agatttgaaa gatgctattt ctagtttaga aaatgataaa
     1321 cttgaacttg agaaaactta tccagtacag tctaggagaa ttagaaagaa aaaagcaaga
     1381 ttattggtag caatacgagg aattaaagta aaacggcaac gtatgacaga tttaatcaat
     1441 cagctatcag acgaaaacca acgtaaaatt ttgaccttac aatatatcga aggagtaaaa
     1501 gacaaacatc tagtagaggt aagcggtcta aaagattata gagaagtctc tagcattaga
     1561 caaaaagcta ttaagaactt agagcggtta caaaagcaac tagaacagcc ccaagcttaa
     1621 atttagccct attttagcgc gtgttttgct ttatccgata aattaaccat acagcaacta
     1681 aaagccctta gaaacgattt taggggctat ttttgataac tgatattaga caaaatatta
     1741 tctttttact tgaatacgtg tagtacgtgt gatataatga tgttgtcagg aggtaaaagc
     1801 gctatgccta tgacacaaaa agaaatggtt aagctactta ccgcccatgg ttggacaaaa
     1861 actaaaggtg gtaaggggtc tcacgttaaa cttgaaaaag taggcgagcg acctataacc
     1921 attccccacg gtgaaattaa caaatacacc gaaaggggta tcaaaaagca agcagggctg
     1981 ttataaaagc agtccctcgc ttgtctttga ttttggaggt aaactatgct tgtcacttat
     2041 ccagctttat tttattatga cgatacagac ggagctaccg ccccttattt tgtcactttc
     2101 ccagactttg aacattcagc aactcagggc gaagatatgg cagacgctat ggcaatggct
     2161 agtgattggt taggtattca tctagctgat tatattgaga atgggcgtga tattcccact
     2221 cctaccccta ttaacgcttt atcattggct gataataacc ctttccgtga tgataaggac
     2281 atagagctag tttatgaccc tagcaagtct tttgtatcta tggttatggt tgacgttgct
     2341 gagtatctag gcagtcaaga gcctgtaaag aaaacactaa ctattccacg ttgggcggat
     2401 actttagggc gtgagttagg gctaaatttc tcacagacct taacagacgc tattgcagat
     2461 aagaaaattc atgcttaaca ctatctaact tatatgatag ggccaaacca gtacactaaa
     2521 acaggtaact gctaaggtgt aaggttagcc ctattttgta tttttacaca ataaaggagt
     2581 aattttaaca tggctaaacc taaagaaaaa tggacaccaa taataaccca cattgtccct
     2641 aaagatactg aactagcaga cggcacaata ttagacaaag aaaccacctt atcacaagaa
     2701 gaattttcta aaaatccccc tgttattcca gacggacatc ctttttataa tatttgggct
     2761 aggataatta gagagaagat agaaaagggt gagctatagc accaagatac agtagaaaac
     2821 agtaaaatac agtagatttg cgcgtgataa ttgcttgaaa ttgatttgtc aaggtcaaca
     2881 aaattgataa ccacgcgcat ttaagctact taatttacgg ttgataaaat cccaatacgg
     2941 taaagtttta accttttcca atttggaaac agttatctat cttttaaaaa tggcatattg
     3001 tgagaaataa ctaacttgtg ccagctatcc gtggtataat atattcacag ctggaggctt
     3061 gcatagtcta gccgattagg gagagttaac ccgtgaggtc atacactccc ttttttggta
     3121 taaaatagct atcagcaaga catcagataa ctaaaaagca ccttatcaag tgtacttaag
     3181 atttgctttt gtaaagtgaa tatgatagca tgaaaatata gtatccttta caggttccac
     3241 cgctattctg atagcaaagg agcggaatat agtgcgacat tgaatgccgc tcagtgataa
     3301 acgtaccagc caatatgtcg ttagttttgc tggtgtcccc tgttggtggt tgaggggata
     3361 gtgaggaggg gtatccctct tttttgtatg gtaattaaga agacctgaaa aaagcacctt
     3421 gtgggggtgc tagttcttgc ctgctgaact catcgtttta ataccttttt tactacccct
     3481 ctagt
//
LOCUS       DBNFEG010000176         3484 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.176, whole genome shotgun sequence.
ACCESSION   DBNFEG010000176 DBNFEG010000000
VERSION     DBNFEG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.176"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..278)
                     /locus_tag="AC1PNG_001871"
     CDS             complement(<1..278)
                     /locus_tag="AC1PNG_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad protein"
                     /protein_id="HIP6702352.1"
                     /translation="MKKKTTLFALTGIILSCHLFLAGCQKTENTAINHQDVQSSNISK
                     VDENTIVVSEIKSNGYMRLYGEESYFFEGPIPYAAHFLKDTLPDSDYK"
     gene            663..1415
                     /locus_tag="AC1PNG_001872"
     CDS             663..1415
                     /locus_tag="AC1PNG_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702353.1"
                     /translation="MENTSTQTFEELVAEQKKKRNRVLRIIITLFTTIIVFFACLALF
                     KYNSGNISGTWRNSQAEQDIVETSIKELQNQLPGIDKTWIDATYTMTVVDKKAVFTAT
                     TSFDIPKIAKLTLDQAYKVMLEEAQKEASSLGISVEEYLTTALGADYEKQLKSFLPTQ
                     AEAEKEFHKYQDEYAKELGATYNQETGTISATFFEGEVDQLFHTVTITKINDAAIKQL
                     TTYDISVKDIISYSRKGNTTIINGNEEFTLEK"
     gene            complement(1934..2464)
                     /locus_tag="AC1PNG_001873"
     CDS             complement(1934..2464)
                     /locus_tag="AC1PNG_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIP6702354.1"
                     /translation="MKQNQLRQLTEISLFAALICVVVFFIRIPFGSQFVHLGNALVVV
                     GVLIFGAKKGALAASLGLGIFDLLSGYASVAWITILESLIVCFVLHLFYEKFLQANDS
                     LSNIILVGIVAALTKIILNLLKYTLTGSLVGGLTPSASFLAALGRILGTYGTAITTII
                     AVPILYQVCKRLYKRK"
     gene            complement(2461..3309)
                     /locus_tag="AC1PNG_001874"
     CDS             complement(2461..3309)
                     /locus_tag="AC1PNG_001874"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HIP6702355.1"
                     /translation="MVQRLLLANDLPGVGKVALATSIPIAAACQVETMLLPTVLLSSH
                     TGGFPDVVIEDMTDLNRAYFRQWKNLNLELAGILSGYCRNPQQLSQLAMYAKQTETPL
                     IVDSVMGDGGKLYTGFTPTYVEAMKELIQSAKLILPNVTEAAFLTGRDYLGACYDRVA
                     IEDLLEALAEQVSADIVLTGISFDDRQIGVAYFQQETREITTYMSKKYPANFFGTGDI
                     LSTLLAVASIQQINLHQAIPLALDFIDKSLERTLALDRDLKFGISFEPFLAELQQAFQ
                     TLKEKL"
BASE COUNT         1191 a          691 c          598 g         1004 t
ORIGIN      
        1 aatttataat cagaatctgg aagggtatct tttaaaaagt gtgccgcata cgggatcgga
       61 ccttcaaaaa agtatgactc ttctccatat agacgcatat agccattaga tttaatttca
      121 gacactacaa tcgtattttc atctactttt gagatattgc tactttgaac atcttgatga
      181 tttattgcag tattctctgt tttttggcaa cccgccaaaa aaagatgaca agataggata
      241 atgcctgtca aggcaaataa ggttgttttc tttttcattt ttgtcctcct aaactcatta
      301 accagttaac aattaactag tatacatttt atttttgatt tttgcaagaa aaaacaactg
      361 acaatgattg ttcgtgaata aattttccat tccacgacaa acgcatgaaa aatttgtttt
      421 ataaaccata ttttcacgac tgctgtcagt ttttagtaga aaaaatgtaa tataatagcc
      481 ttggaaatgg gggtatcctt gttaaagtgc aagttttaca aaaaccttta aaaaaatgca
      541 tacaacaaga aaatagttac ataccttttt cttcatgcgg attgcatgtt tccatcccta
      601 cgatcattga caaagcaaac aaactatagt atattaaaaa tagggaaata aaaggagaat
      661 atatggaaaa tacatctact caaactttcg aagaactcgt tgcggaacag aaaaagaaac
      721 gtaatcgcgt ccttagaatt attatcacac tctttaccac tattatcgta ttttttgcct
      781 gtcttgcatt attcaaatac aacagtggca acattagcgg tacgtggcgt aattcccaag
      841 ctgaacaaga tattgtcgaa accagtatca aggaattgca aaatcaactt ccaggtatcg
      901 ataaaacgtg gattgatgca acttatacga tgacagtcgt agataagaaa gctgtcttta
      961 ccgctacaac aagctttgat attcctaaaa tcgcaaaatt aaccttggat caagcctata
     1021 aggtgatgtt agaggaagca caaaaagaag caagttctct gggaatttcc gtagaagaat
     1081 acttaacaac cgctctcgga gcagactatg aaaagcaatt gaaatcattt ctacctacac
     1141 aagctgaggc ggaaaaagaa tttcataagt accaagacga atatgctaaa gaactaggtg
     1201 ccacatacaa tcaagaaact ggtactatct ctgctacctt tttcgaggga gaagtcgatc
     1261 agctcttcca taccgtgacc attacaaaaa ttaatgacgc agcaatcaaa cagctgacta
     1321 catacgatat ttccgtaaaa gatattattt cctattctcg caagggaaat accaccatta
     1381 ttaacggaaa tgaagaattt actttagaaa aataataaaa aaagctggga aagtcatccc
     1441 tgatggttcc gacctcaccc acagaataat gaaaataaat atgaagccct cactagatgt
     1501 ataatcttag tgagggcttt gtgcttgacg agttaatgat gttagtcatc gccagaaaaa
     1561 ctaacccact ctattgatta gctctaaaaa atatgcaata gacatgagta taagaactag
     1621 ccaataaaaa cacagataga ttcaagctga ttttgatgaa atacagccga attgtctgtg
     1681 cttttatttt tgtctctact atagatagga caacattgcg gcgatatttg aaagaacttg
     1741 taccaatact caatgaaaat caaaagtagc ctaggaaacg aagccgaagg tagaactgga
     1801 gttatcaagg caagttgaca acggataatt ttgattttcg aagagtataa tctcctgttt
     1861 gtttcgccac ttacagtgta gtccttctga cctttttttg catattgaat ttaactaatg
     1921 tcactaatat aaattacttt ctcttatata accttttaca tacctgataa agaataggta
     1981 cagcaatgat ggtcgtaatt gcagtgccat aggttcctaa aatcctaccc aaagcagcta
     2041 aaaatgatgc cgatggcgtt agtccaccga ctaaactacc cgtcaatgtg tacttcaaca
     2101 ggttcaagat aatttttgtg agggctgcaa ctatcccaac caagataata tttgatagac
     2161 tatcgtttgc ctgtaggaat ttctcataaa ataagtgtag gacaaaacag acaatgagtg
     2221 attctagaat ggtaatccaa gcaacggagg catatcccga cagtaaatca aagatgccta
     2281 gaccgagtga agctgccagc gccccttttt tggcaccaaa gatcaatact cctactacca
     2341 ccagagcatt gcctaaatgg acaaattgac tcccaaaagg aatgcgaata aagaagacaa
     2401 ccacacaaat aagggctgca aataggctaa tttctgttaa ttgacgcaat tgattctgtt
     2461 tcataatttc tcctttaatg tttgaaatgc ctgctgtaat tctgctagaa aaggttcaaa
     2521 ggaaatccca aatttcaagt ctctatccaa agcaagggta cgctctaaac tcttatcgat
     2581 aaaatctaaa gctagaggga tagcttgatg gagattgatt tgttggatgg atgctacagc
     2641 caacaaggta gatagaatgt cacctgttcc aaagaaattt gctggatatt ttttagacat
     2701 ataggtagtt atttcccttg tttcctgctg aaaataggct acacctattt gtctatcatc
     2761 aaaggaaatc cctgtcagta caatatctgc ggatacctgc tctgctaaag cttccagtaa
     2821 gtcctcaata gccaccctat cgtagcaagc tcccaaataa tccctccctg tcaaaaaagc
     2881 tgcctctgtt acattgggga gaatgagctt tgccgactgt atcaactctt tcatggcttc
     2941 tacataggta ggggtgaaac ctgtatacaa cttccctcca tcccccatga ccgaatcaac
     3001 aatcaatggt gtctctgttt gtttagcata catagccaat tgacttagtt gctgaggatt
     3061 tctacagtag ccagacaaga tgcctgccaa ctccaaattc aagttcttcc actgtctaaa
     3121 ataggctcga ttcaagtcag tcatatcttc aatgacaaca tctgggaaac ctcctgtgtg
     3181 tgaggataaa agaactgtag gtaaaagcat tgtttctacc tgacaagcag ccgcaattgg
     3241 gatactagtt gctagtgcaa ccttaccaac accaggtaag tcgttcgcaa gcaataatcg
     3301 ctgaaccata acaacctcct aaattatttt tcttaaacaa aattgtaaag gattatcata
     3361 catttttcaa tcttacatat ccacaactgt atggggacag acagctgaaa ccttacaaca
     3421 ccaggctcaa attattacat aagaaaaaca gtaacaactc tttgctactg tcatcctttt
     3481 caat
//
LOCUS       DBNFEG010000177         3448 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.177, whole genome shotgun sequence.
ACCESSION   DBNFEG010000177 DBNFEG010000000
VERSION     DBNFEG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.177"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(70..384)
                     /locus_tag="AC1PNG_001875"
     CDS             complement(70..384)
                     /locus_tag="AC1PNG_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HIP6702356.1"
                     /translation="MALRHYQSHEYQYEEYIPKEKKANFQTYQAKNTNKERLKELLFF
                     VNIAFFSLITVIASYVYLSVGIPVFVAIILASATGFLGLRLVQIGLKKKFKPKQVIRK
                     NE"
     gene            545..1474
                     /locus_tag="AC1PNG_001876"
     CDS             545..1474
                     /locus_tag="AC1PNG_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIP6702357.1"
                     /translation="MEKIIITATAESIEQVKELLVAGVDRIYVGEADHALRIPTNFTY
                     DELREIAELVHSAGKELTVAANALMHQDMMDNIKPFMELMREIKVDYLVVGDTGIFYI
                     NKRDGYNFKLIYDTSVFVTSSRQINFWKDHGAVEAVLAREIPSEELFDIAKNLEMPAE
                     ILVYGASVIHHSKRTLLQNYYNFTKADETDLSRGRGLFLAEPSDPDSHYSIFEDKHGT
                     HIFINNDIDMMTKLSELDEHGYHNWKLDGIYCPGQNFVEIVKLFVQARDLIEKGEFTY
                     DQAFLLDEQVRKLHPAERGLDTGFYEFDRNKVK"
     gene            1652..2938
                     /locus_tag="AC1PNG_001877"
     CDS             1652..2938
                     /locus_tag="AC1PNG_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIP6702358.1"
                     /translation="MSKTLKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFTMDEMREGIEYAHARGAKVYVAANMVTHEGNEEGAGAWFRELRDMGLDAVIVSDP
                     ALIVICATEAPGLEIHLSTQASSTNYETFEFWKELGLTRVVLAREVTMDEVAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSKRDANRGGCSQSCRWKYNLYDLPFGEERRSI
                     KGEIPEEFSMSAVDMCMIDHIPDMIENGVDSLKIEGRMKSVHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLIDEMWKVAQRELATGFYYSPPSENEQLFGARRKIPEYKFIAEVV
                     AFDKETMTATIRQRNVINEGDQVEFYGPGFRHFETTIQDLHDSNGEKIDRANKPMELL
                     TIKLDREIYPGDMIRACKSGLINLYQEDGQSLTIRA"
     gene            complement(3151..>3448)
                     /locus_tag="AC1PNG_001878"
     CDS             complement(3151..>3448)
                     /locus_tag="AC1PNG_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702359.1"
                     /translation="EQITVFLEHGKAPKSIEPDSKEEIKNNLELALYKKAAVHVIYGD
                     RSFTGDIVKYDTDCQRIIMKNFKRQVTSIIKIEDIKKVTLVPDSIKTSQQQTKG"
BASE COUNT         1008 a          686 c          745 g         1009 t
ORIGIN      
        1 caattgttca cagacggcgg tagttcattc aaaaaaggca gaggataact tctctgcctt
       61 tatacttcat tattcatttt ttcttataac ttgtttgggt ttaaatttct tctttagccc
      121 aatttgaacc aaacgtagtc caagaaaacc cgtcgcactt gccaatatga tggcaacaaa
      181 aacaggaatg cctacggata gatagacata actagcgatg actgttatca aactaaaaaa
      241 tgcgatatta acaaaaaaca acaattcctt tagacgttct ttattggtat tttttgcctg
      301 ataggtttga aaattagcct ttttctcttt cgggatatac tcttcgtact ggtactcatg
      361 ggactgatag tgtcttaatg ccatatcgtc tccttttctg ctccttaaag aataccataa
      421 cattgctgaa tttttatgtc atttatatta caataatgtt acaagagaaa gattagaaaa
      481 gtctgaacat taaaatacag gtagtcactt atcagatatt tctctttttg atataattat
      541 ctttatggaa aaaattatca ttacagcaac agctgaaagt attgagcagg tcaaagaact
      601 gcttgtagca ggcgttgacc gcatttatgt aggtgaagct gaccatgctc ttcgcatccc
      661 aacaaatttt acctatgatg aactcaggga aatagcagag ctagttcata gcgctgggaa
      721 ggaattgacg gtcgctgcca atgccctcat gcatcaagat atgatggaca atatcaaacc
      781 atttatggag ctaatgaggg aaatcaaggt tgattatctg gttgtcggag atactggaat
      841 tttctatatc aataaacgtg atggctacaa tttcaagcta atctatgata catctgtctt
      901 tgtgacttcc agtcgtcaga ttaacttttg gaaagatcat ggagcagtag aggctgtttt
      961 ggcaagagag attccatcag aagaactctt tgatattgct aagaacttgg aaatgccagc
     1021 ggaaatcttg gtctacggcg cctctgttat tcaccattct aaacgaacct tgttacagaa
     1081 ttattacaat tttacaaagg ctgatgagac agatttatca cgtggacgcg gacttttctt
     1141 agctgagccg agcgacccag atagccacta ttctatcttt gaggacaagc acggtaccca
     1201 tatctttatc aacaatgaca ttgatatgat gaccaagctg tcagaattag atgaacatgg
     1261 ttatcataat tggaaacttg atggtatcta ttgccctgga cagaactttg ttgaaattgt
     1321 taagcttttt gttcaagcaa gagatttgat tgaaaaaggc gaattcactt atgatcaggc
     1381 cttccttttg gatgagcagg tacgcaagct ccatccagct gaacgtggct tagatacagg
     1441 cttctacgaa tttgaccgca ataaggttaa ataacaatat ttatagcaca atttatttac
     1501 tttaacctga cagagttagc tctgtcactt attatgtaag gtcagagcac gaaaacgcct
     1561 ttgagcgatt ttctaaactc tgcactaggt ttccacatgg aaaagtatcg tttcatgtgg
     1621 aaaccgtcgc aaatagagga gattttacat gatgtcaaaa acattaaagc gtcccgaggt
     1681 cttgtcacct gctgggactt tggagaaatt gaaggttgcc attgattatg gtgctgatgc
     1741 ggtctttgtt ggcggccagg cctacggtct gcgtagccga gctggtaact ttaccatgga
     1801 cgagatgcgg gaagggattg aatatgccca tgcccgtggt gccaaggtct atgtggctgc
     1861 caacatggtc acccacgaag gtaatgaaga aggagcagga gcctggttcc gtgaattgcg
     1921 tgacatgggc ttggatgccg ttatcgtatc tgacccggct cttattgtta tctgtgctac
     1981 tgaagcgcca ggccttgaaa ttcacctgtc aacccaggct tcttccacca actatgagac
     2041 cttcgagttc tggaaggaat tgggcttgac tcgtgtcgtc ctggcccgcg aagtgactat
     2101 ggacgaggtg gctgaaatcc gtaaacggac agacgttgag attgaggcct ttgttcacgg
     2161 agctatgtgt atttcctatt caggtcgctg cgttctctct aaccacatga gcaaacgcga
     2221 tgctaaccgc ggtggctgct cccagtcttg ccgttggaag tacaatctct acgacctccc
     2281 atttggcgaa gaacgccgtt cgattaaggg ggaaatccca gaggagttct ccatgtctgc
     2341 agtggacatg tgtatgatcg accatattcc agatatgatt gaaaatgggg tcgatagcct
     2401 gaaaatcgaa ggccgtatga aatctgtcca ctacgtatca acagtgacca actgctacaa
     2461 ggctgcggta gatgcctatc ttgaaagccc agaaaagttt gaagcaatca agcaagacct
     2521 gattgatgag atgtggaagg ttgcccaacg tgaattggcg acaggtttct actatagccc
     2581 gccgagtgaa aatgagcagc tctttggagc ccgccgtaag attccagaat acaaattcat
     2641 cgccgaagta gtggcttttg acaaggaaac catgactgca accattcgtc agcgtaatgt
     2701 tatcaacgaa ggtgaccaag tagagtttta cggaccaggt ttccgtcatt ttgaaacgac
     2761 tattcaggac ttgcatgatt caaatggaga aaagattgac cgcgccaaca agccgatgga
     2821 actcttaact attaagctgg accgtgaaat ctatcctggt gacatgattc gtgcctgcaa
     2881 atcaggcctc atcaacctct accaagaaga tgggcaatct ctaactattc gtgcataaac
     2941 ataaaaaggc tgaaatttca gcctcagaac gtagataaac tcttaaaaat ataaaaaatt
     3001 gagaagtttg cgacagatag caagtcatta aactcttaga aacattgata tttcggcgtt
     3061 tctgagagtt tttaattttc aacataaaga aaaaagattg aagaaaattg tccaaaatgg
     3121 actttttctt caatctgcaa ggctgaaatt tcagcctttt gtttgttgtt gagaagtctt
     3181 gatgctatct ggaaccaagg tgactttttt gatgtcctcg atttttataa tgctggtaac
     3241 ctgacgtttg aaatttttca tgataatccg ttggcaatcg gtatcatatt ttacaatgtc
     3301 acctgtgaaa ctacgatcgc catagatgac atggactgca gcttttttat ataaagccag
     3361 ctccaagtta ttcttgattt cttccttgct atctggctca atagacttag gagctttgcc
     3421 atgttctaaa aagacggtga tctgctcg
//
LOCUS       DBNFEG010000178         3433 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.178, whole genome shotgun sequence.
ACCESSION   DBNFEG010000178 DBNFEG010000000
VERSION     DBNFEG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.178"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..1604)
                     /locus_tag="AC1PNG_001879"
     CDS             complement(<1..1604)
                     /locus_tag="AC1PNG_001879"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004224101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIP6702360.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITDALKDKIKRAARTTDADVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSDNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEQPAG
                     QGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNMQAQNMDQIVCDHLKLDVPPADMTE
                     WTAMVNKVLNLQKKVKIALVGKYVELQDAYISVVEALKHSGYANDAAIELDWVNANDL
                     TAENVAERLGQAQGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCVEFA
                     RHVLGLEGANSFELDPETKYPIIDIMRDQIGVEDLGGTLRLGLYPSKLKRGSKAAAAY
                     DNQEVVQRRHRHRYEFNNDFREQFEKAGFVFSGVSPDNRLVEIVEIPENKFFVACQYH
                     PELQSRPNRPEGLYTAFVSAAIENEK"
     gene            complement(1797..2378)
                     /gene="rpoE"
                     /locus_tag="AC1PNG_001880"
     CDS             complement(1797..2378)
                     /gene="rpoE"
                     /locus_tag="AC1PNG_001880"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HIP6702361.1"
                     /translation="MELNVFAGQEKSELSMIEVARAILEERGRDNEMYFNDLVNEIQN
                     YLEKSNSEIRAALPTFYSDLNVDGSFIPLGENKWGLRSWYAIDEIDEEVITLEEDDED
                     APKRKKKRVNAFMDGDDDAIDYGHDDPEDEDNYPGSVSSEYDDENPDDEKDEVESYDS
                     EINEIIPDDELDEEDVDLGEDDDEYSDEEVVDE"
     gene            complement(2459..3314)
                     /locus_tag="AC1PNG_001881"
                     /pseudo
     CDS             complement(2459..3314)
                     /locus_tag="AC1PNG_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
BASE COUNT          938 a          737 c          663 g         1095 t
ORIGIN      
        1 tatttttcat tctctatagc tgctgaaaca aaggctgtat acaaaccttc agggcgatta
       61 ggacgagatt gtagttctgg atggtattgg caggcaacga agaatttatt ttctggaatt
      121 tcaacaattt ctaccaaacg gttgtctggt gacacacctg agaaaacaaa acctgctttt
      181 tcaaattgtt cacggaagtc attattaaac tcataacgat gacgatggcg acgttgtaca
      241 acttcttgat tatcataagc agctgcagcc ttggagccac gtttgagctt gcttggataa
      301 agacctaaac ggagagttcc tcctaaatct tccactccaa tttgatcacg catgatgtca
      361 ataattggat atttggtttc tgggtctaat tcaaagctgt tagcaccttc taaaccgaga
      421 acgtgacgag caaattctac acaggtcagc tgcattccca agcagacccc tagcattggc
      481 acgtcattct cacgagcata acgaatggct tgtattttcc cttctgtacc acgttgtcca
      541 aagccaccag gaacgataat tccttgtgct tgtcctagac gttcagctac gttttctgct
      601 gtcaaatcat ttgcattgac ccagtccaat tcgatagcag catcatttgc atatcctgag
      661 tgttttagag cttcaactac agaaatatag gcatcttgta attctacata cttaccaact
      721 aaagcgattt ttactttttt ctggagatta agaaccttgt ttaccatcgc tgtccactct
      781 gtcatatctg ctggaggtac gtcgagcttc aagtgatcgc aaacaatttg atccatgttc
      841 tgcgcttgca tatttaatgg gatttggtaa agatgttcga catccaagga ctcaataact
      901 gcttctggtg caacatcaca gaattgtgcc agtttatttt taattccttg tccagcaggc
      961 tgctctgtcc gaattaccaa catgttgggc tgaataccta gaccacgcaa ctcttttaca
     1021 gaatgttgtg ttggcttagt tttcatttcg cctgctgctt tgagataagg caagagtgtt
     1081 gtatggatat acataacatt gtctgaaccg acatctgcct tcatctgacg aagcgcttca
     1141 aggaatggca agctttcaat atcacctact gtaccaccca cttccgtaat gatgacatct
     1201 gcatctgtag tacgagctgc acgcttgatt ttgtctttca aagcatctgt aatgtgtgga
     1261 ataacttgaa ctgttgcccc taagtattcc cctttacgct ctttacggag gacttcgcta
     1321 taaattttac ctgttgtaac atttgaatat ttgttcaggt taatgtcgat gaaacgctca
     1381 taatgcccaa ggtccaagtc ggtttctgcg ccgtcgtccg tcacaaaaac ttcaccgtgt
     1441 tgatacggac tcattgttcc cgggtcaata ttgatgtatg gatcaaattt ttggattgtt
     1501 accttgagac ctcggttctt caataaacga cccaggctgg ctgctacaat ccctttgccg
     1561 atagaggaaa caacgccacc tgttacaaaa atatactttg tcatgcaaac tccttttcta
     1621 cgattcaggc taataggaag caatttgtgg ggatacttcc tgttaacttg aagaaaaaac
     1681 aaaaatagct ccctatcgct agggagctcc gacctcaaaa gaggtgcccg ataatatcct
     1741 atcgtaattc agtagtcttg tcaaattatt tttaagaatc tatggggata taattcttat
     1801 tcgtcgacga cctcttcatc tgaatactcg tcgtcatctt cacctaaatc aacatcctct
     1861 tcatccaatt cgtcatctgg gataatttcg ttgatttctg aatcatagga ctcaacttca
     1921 tctttttcgt catctggatt ttcatcatcg tattctgatg atacactacc cggatagtta
     1981 tcctcatctt ccggatcatc atgtccatag tcaatcgcat catcatcacc gtccatgaag
     2041 gcattgacac gtttcttctt gcgttttggt gcatcttcgt catcttcttc aagagtgatc
     2101 acttcttcgt cgatctcatc gattgcgtac caagaacgta agccccattt gttttcgcca
     2161 agtggaatga agcttccatc cacattcaaa tcagaataga aggttggaag ggctgcgcga
     2221 atctcgctgt ttgatttttc aaggtagttt tgaatttcat tgaccaagtc gttgaaatac
     2281 atttcgttgt cacgtccacg ttcttctaaa atagcacggg ctacttcaat catggaaagt
     2341 tcgctttttt cttgtcctgc aaatacgtta agttccaagg ctgttctcct tttttgtctt
     2401 tcctttctat tttacgctaa aaaaggcaaa tagtcaaaga taatctttac ccgtggtgct
     2461 agttcatttc aaaatagcgt aagttatgag ctagaataat ttgttccatt cgtaactgta
     2521 gtccggatag gcttctagct aatgtatgtt cgatgtcaaa gagacgacac agttcagaaa
     2581 aacgtgtttc aatagttcgt ctcatggtca ttactttcca attgttatgt tcttcagcct
     2641 ctgccatgtt ttgtcgaaat ggcgtccata gatggtaacc ttgttgttct aagtccttct
     2701 tgagttcact gctcaggtag cctaaatcac ctaggataac cgattgctta cacccttcta
     2761 atagttcata aaccaccttg atatcgtgaa cagaggcagg tgtgacaaca tagttcagaa
     2821 taaaaccaga taaggtcacc agcatatgaa ccttgaagcc gtagaaccaa agatgtttag
     2881 aggcgttgta gccaatatcg gctaatcctt taaaaatggt agctctgtga ttgcgaacag
     2941 gttggcaaag gaaaactatc tataatggct agtttatcag atggaagcat ttcactcata
     3001 gcttttcgga ttaattggac gagccaaatc aattgttttg accgacgatt gaaacggctt
     3061 ctttctagta actgaccaca aggaaagagg tggcagatgc tatagaaatg acgctgtgac
     3121 gttatgccta gttcggcctg taacaagagc aagacaagga gtgagtcatc tgaaactttg
     3181 gctaagccaa cattacgccg atgtttaaag gaatctggac aatagttttg gtagaagtga
     3241 tgacaaattt gtgataattg gcatacattc cattgcaaat gatgagattt agcggtatac
     3301 tgtaagtggc tcatttggac taacctcctg tatgtttcgt cacttacagt atagtccatt
     3361 tgggcttttt attgtatttt gaaatgaact agcaccacgg gtaatcttta tcgtttttct
     3421 gtaaaaaaat acc
//
LOCUS       DBNFEG010000179         3432 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.179, whole genome shotgun sequence.
ACCESSION   DBNFEG010000179 DBNFEG010000000
VERSION     DBNFEG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.179"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..914
                     /locus_tag="AC1PNG_001882"
     CDS             <1..914
                     /locus_tag="AC1PNG_001882"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIP6702362.1"
                     /translation="GTITIENRNDAQGTFDVRVTNVSSPKDIASVLLPTWSQSDDIRW
                     YEATRQSDGSYKLTVNKKDHKYRTGTYTVHLYYKDSNGGLTGAGGTTTHLSNPSAQRS
                     YTVYIDPGHGGRDSGASYGGVHEKNLALSVSNKLRENLLQYGINVLMTRTGDYDVDFK
                     TERSRMTNASNADLFISIHFNATGAGVSNATGIETYWYQYNPEYQPKINKEMHNNPTR
                     LAESEILANKVQESLIKETGAVNRGVRRETFAVLRETAIPAILVELGFMDNPSELQVI
                     KQDSYHTRLAKALAQGVMNWYGAVEGK"
     gene            992..1420
                     /locus_tag="AC1PNG_001883"
     CDS             992..1420
                     /locus_tag="AC1PNG_001883"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013954.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HIP6702363.1"
                     /translation="MEQSSKANSILSSVMLSQAILESAWGTSYLATHGNNLFGIKADA
                     AWTGATIEIITNEYRDGEKKQEKHLFRKYNSWNESVADYAKFFTSTPWRIKNYQSFRE
                     ATDYQQAILALRQSGYATDPKYGEKLRSIIENYKLYLLDN"
     gene            complement(1480..>3432)
                     /locus_tag="AC1PNG_001884"
     CDS             complement(1480..>3432)
                     /locus_tag="AC1PNG_001884"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIP6702364.1"
                     /translation="FTFPRLYRNELVLFNSNFNKMETQQLLVALTFLIFFGLLGYKQL
                     RGHISKAFKTISLSVIYLLVPIFSLFIIENSYSQFSTLSSNSLWNNLIIIGVLYLILF
                     FTTNLRFASLLILSASVFIGISNQLLIDSRGAPLLFYNLFQIIDGLNVASSVAININN
                     RMLQSMVFSYILLTFFFFIPKLYLPKLLPSRTFYSSYDFKWPKRFSRILLGYITLITI
                     VPMINKTVVSNANISLNYWRMYVTYGQFGLPLSLASFYEDSKITKPEGYSVPKLNEVL
                     EKYPPETEKQTIRPNIIFIQNESQSDFSNLQGLNMEPDPLSNQHALTDNTIHGTLNVS
                     VFGGGTANTEYEVLTSNPISLLSSNLFPYQQIITQERPSFATYLKNKNYDTVALHPQS
                     GNNYNRNAVYPLLGFNKSYFLDSEPAISSLAPLTIDRGWPSDQFLFNGIKELYTQKGD
                     QPLFSFVVTMQGHGGYPSTEEIYPREVSINGSTSEYLAETEFLTSMKRTDEAFADLIT
                     FFSTYKEPTVIVMYGDHQPSLTQEFYAQFMDENNPAAKYSTPLVIWSNFDIRERESTT
                     ISPNYLVPYLMDILSESDYALPRSPYQQFLSDMQIEAPIITSWGNIDNNGQQIEDLST
                     LPLYQTYLQLEYNSAIDKQPLTDLYE"
BASE COUNT         1113 a          546 c          730 g         1043 t
ORIGIN      
        1 caggtaccat cacaattgaa aatcgtaatg atgcccaagg tacctttgat gttcgtgtta
       61 ctaatgtttc atcaccgaaa gatatagcaa gtgttctctt acctacctgg tcccagtcag
      121 acgatatacg ttggtatgaa gctactcgtc agtcagatgg cagttataag ttaactgtta
      181 ataagaagga ccataagtat cgtacaggta cctacacggt tcacttatat tataaggatt
      241 caaatggtgg tttgacaggt gcaggtggaa caacaactca tttatcaaat ccgtcggcac
      301 agcgatccta cacagtctat attgacccag ggcacggagg gcgagactcg ggtgcttctt
      361 acggtggcgt acatgaaaaa aatcttgcat tgtctgtttc aaataagttg cgtgaaaatc
      421 tcttgcaata tggaatcaat gttttgatga cccgtacagg tgattatgat gtagacttca
      481 aaacagagag gtcacgaatg acgaatgcaa gcaacgcaga cttatttatt agtatccatt
      541 ttaatgcgac gggtgcagga gtttcgaatg cgactggtat cgaaacatac tggtatcaat
      601 ataatccaga ataccaacct aaaattaata aagaaatgca taataaccct actcgtttag
      661 ctgaaagtga gattttagcc aacaaggtcc aggagagttt aattaaagaa acaggtgcag
      721 ttaaccgtgg tgtaagaaga gaaacttttg cagtccttcg cgagacagca attcctgcca
      781 ttctagtgga gctaggattt atggataatc cttcggagtt gcaagttatc aaacaagatt
      841 cttaccacac tcgccttgcg aaagcattgg cacaaggtgt aatgaattgg tatggtgctg
      901 ttgagggaaa gtagaaattg caggtacagg tattgaaaca ggaaaacttt ctaatgatca
      961 aaaaagtttc atagccaatc tagtacctgc aattgaacaa agttcaaaag ctaattctat
     1021 cctttcgagc gtcatgttat ctcaagccat cctcgaaagt gcatggggaa cgtcatactt
     1081 ggcgactcat gggaataatc tttttggcat caaagcggat gctgcgtgga caggtgctac
     1141 aattgaaatc attaccaatg aataccgtga tggtgagaag aagcaagaaa agcacttgtt
     1201 tagaaaatat aattcatgga atgaaagtgt agcagactat gctaagttct ttacctctac
     1261 tccgtggaga ataaaaaatt accagagttt cagagaggca actgattatc aacaagcgat
     1321 tcttgcgcta agacaatcag gatatgctac tgatccgaaa tatggtgaaa aattacgatc
     1381 aattatcgag aattacaagc tttatctttt agataattaa gttttttggg tgattgatga
     1441 taaaatagcc ttgctcaaaa ccgagcaagg ctatttttat cactcatata aatctgttaa
     1501 aggttgtttg tcaatggcac tattgtattc tagttgtaag taggtttggt aaagtggtag
     1561 ggttgatagg tcttcaattt gttggccatt attatcgata tttccccagc tagtaatgat
     1621 aggtgcttcg atttgcatat cactcaaaaa ttgttggtaa ggtgatcgag gaagggcata
     1681 gtcggattcg gataaaatat ccatcaaata tggaacgaga tagtttgggc tgattgttgt
     1741 agattcgcgc tccctaatat caaaattcga ccaaatcact aaaggggtag aatatttagc
     1801 ggcaggatta ttttcatcca taaattgtgc gtaaaattcc tgagtcaaac taggttggtg
     1861 atcgccgtac atgacgatga ctgttggctc tttgtaagtg ctaaagaaag taattaaatc
     1921 tgcaaatgct tcatctgttc ttttcatgct ggtcaaaaat tctgtttcag ctaagtattc
     1981 agatgttgaa ccattaatgc tgacttcgcg aggataaatt tcttccgtac ttggatagcc
     2041 tccatgtcct tgcatggtaa caacaaagct aaatagaggc tggtctcctt tttgggtata
     2101 aagttctttt attccgttga acaagaattg atcagatggc cagccacgat cgattgttag
     2161 tggtgctaag ctactaatag caggttccga atctaaaaaa taagatttat taaaccccag
     2221 tagaggatac actgcattgc ggttgtaatt atttccggat tgaggatgta gagctacagt
     2281 gtcgtaattt ttatttttta gataagttgc gaaacttgga cgttcttgtg tgataatctg
     2341 ctgatatggg aataaattag aagataataa actgattggg ttacttgtca gcacttcata
     2401 ctcggtattt gcagtaccac cgccaaagac tgagacattg agggtgccat gaatagtatt
     2461 gtctgttaat gcatgttggt ttgatagcgg atcaggctcc atatttaagc cctgtaagtt
     2521 tgaaaaatca ctctgtgatt cattctgtat aaagataata ttaggtctga ttgtttgttt
     2581 ttctgtttca ggagggtact tctcaagaac ttcattaagt ttgggaactg aatatccttc
     2641 aggcttagtt attttactat cttcatagaa agaggcgagt gagagtggta gcccaaattg
     2701 accataggtg acgtacattc tccagtagtt taatgatata tttgcattag agacgacagt
     2761 tttattaatc attggaacga ttgttattaa agttatatag cccaataaaa ttctgctgaa
     2821 acgtttcggc catttgaaat cataactaga ataaaacgtt ctagaaggta gaagttttgg
     2881 taaatataat ttagggataa agaaaaagaa ggtcaataag atataagaaa ataccatgct
     2941 ttgtagcatt cgattgttga tattaattgc tacgctagaa gctacattta atccgtctat
     3001 gatttgaaaa agattataaa ataatagagg agcaccacgg ctgtcaatca aaagttggtt
     3061 acttatacca ataaatactg aagcggatag aattaatagg ctggcaaatc gtaagttcgt
     3121 tgtaaagaac agtattaggt agaggactcc gataatgatt agattgttcc aaagactgtt
     3181 tgaggaaaga gtagagaatt gtgagtagct attttcaata ataaataatg aaaaaatggg
     3241 aactagaaga taaatcactg atagactaat cgttttgaag gctttagaga tatgacctcg
     3301 gagttgtttg tatcccaaaa gaccaaaaaa tatcagaaaa gttagtgcga ctaatagttg
     3361 ttgagtttcc attttattaa agttcgaatt aaatagcact aattcattgc gatataaacg
     3421 tggaaatgta aa
//
LOCUS       DBNFEG010000180         3430 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.180, whole genome shotgun sequence.
ACCESSION   DBNFEG010000180 DBNFEG010000000
VERSION     DBNFEG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.180"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(18..1022)
                     /locus_tag="AC1PNG_001885"
     CDS             complement(18..1022)
                     /locus_tag="AC1PNG_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase AlkZ-like family protein"
                     /protein_id="HIP6702365.1"
                     /translation="MHHNTWKGIILQKQGLLKPQSVQQICQNLNGLQAQFQPYVHIGF
                     RNRMTNEAFQNQDWQASLTRQWSLRRTVHAYLKSEIPLYIHEGRMASVDYLETVSWDG
                     ALPQVKKKFYQVILEALETGPMTREELKILCRAENIGVEEEKQVFNAWGGLLRYMVER
                     GEIYQEYGVKRFHRLRDFQPLPKKEAELEIARRYFTGFGPVSLADARYYFKENKSTVL
                     KWMEQLDLKTVEVAGEERFYLGELEEAELPDCLFVAGFDQLLLGYEKKANPFFNPKYI
                     RNIYTLTGIVKPVVFYKGRFVATWKRDKGTILLDIFEDITQQEEKELEHYRQMFEKIL
                     "
     gene            complement(1087..1923)
                     /locus_tag="AC1PNG_001886"
     CDS             complement(1087..1923)
                     /locus_tag="AC1PNG_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIP6702366.1"
                     /translation="MIKEFNATVRYLESVLESEIDEREFLRISGYSVPMFSRLFSILT
                     DMSLSEYVRLRKLTRAAMDIREGQAKIIDIAFKYGYESSDSFAFAFKQFHQISPSQVR
                     KGLPFKVLLPLQLSLTIQGGKEMDVRIEKKAGFTVAGLLRTAISNEVCPQVWEDLFKN
                     HTYEELAALGSGQSFGVCTLAQADETINYMAAYDVTDSEKATKLGLELLDVHEADYAI
                     FTLIGPVPQSIHAGWRYALETFFPEHGYRHSGAPDFEYYFEGDMSSPDYQMELWIPIV
                     KA"
     gene            complement(2000..3334)
                     /gene="trmFO"
                     /locus_tag="AC1PNG_001887"
     CDS             complement(2000..3334)
                     /gene="trmFO"
                     /locus_tag="AC1PNG_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HIP6702367.1"
                     /translation="MSQTHINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKPTPQHK
                     TDKFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRAGEAHRVPAGGAMAMDRENF
                     SQAVTDEIHNHPLIEVIRGEITDIPDDAITVIATGPLTSDALAEKIHALNGGDGFYFY
                     DAAAPIVDSSTINMDLVYLKSRYDKGEAAYLNAPMNKEQFTAFYEALISAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPEDYKGPRDGEYKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     FATKKNPNLFFAGQMTGVEGYVESAASGLVAGINAVRRFRGEEPVIFPQTTAIGALPF
                     YITHTESKHFQPMNVNFGIIKELDGPRIRDKKERYEAIAERSLKDLEEFLTV"
BASE COUNT          953 a          809 c          695 g          973 t
ORIGIN      
        1 aaaatagtta gttaatgtca cagaattttc tcaaacattt ggcggtaatg ctccaattct
       61 ttttcttctt gttgtgtgat atcttcaaaa atatctagca aaatagtacc tttatctcgt
      121 ttccaggttg ccacaaatcg ccctttataa aatacaacag gtttgacaat acccgtcaaa
      181 gtatagatat tacggatgta tttaggatta aaaaagggat tagctttctt ttcatagcct
      241 agcaaaagtt ggtcaaatcc tgcaacgaag aggcagtcag ggagttctgc ttcttctaat
      301 tctcccagat aaaagcgttc ttctcctgct acctcaacgg tttttaaatc taattgctcc
      361 atccatttta ggaccgtaga tttattttcc ttgaaataat aacgagcatc cgctaaactg
      421 actggcccaa aacctgtaaa ataccgtcga gcgatttcca gctctgcttc tttctttggt
      481 agtggttgaa agtcacgtaa ccgatgaaac ctttttaccc catactcttg gtaaatttct
      541 cctcgttcaa ccatataacg aaggagacca ccccatgcat tgaaaacttg tttctcttct
      601 tctactccaa tgttctcagc tcggcaaaga attttcaact cttcacgagt catagggcca
      661 gtttctaagg cttccaaaat aacttgataa aattttttct taacctgtgg tagagcacca
      721 tcccaactta ctgtttcaag ataatcaacg cttgccattc tcccttcatg aatatagaga
      781 ggaatttccg atttgaggta ggcatgaact gtccgtcgaa gtgaccattg gcgtgtcagt
      841 gaggcttgcc agtcctgatt ttgaaatgct tcattggtca ttcgattacg aaaaccgata
      901 tgtacatagg gctgaaattg tgcctgcaaa ccattgagat tttggcagat ttgctgaaca
      961 gactgaggtt tcaacagacc ttgtttctgc agaataattc ccttccaagt attgtggtgc
     1021 atcgtatttc tcctccgctt tctaatccca gtataacaca aaagacaccc gaaggtgtcc
     1081 tgtacattat gctttcacaa tgggaatcca gagttccatt tggtagtctg gggaactcat
     1141 gtctccttca aaatagtact caaaatctgg tgcacctgaa tggcgatagc cgtgttcagg
     1201 aaaaaaagtt tctagcgcat aacgccatcc tgcatgaata gattgaggaa cagggcctat
     1261 tagggtaaaa atagcatagt cagcttcgtg aacatcaaga agttctaagc ctaacttagt
     1321 cgctttttca ctatctgtga catcataagc agccatataa ttaatcgtct cgtccgcttg
     1381 agctaatgta caaactccaa aggactgccc acttcctaga gcagctaact cttcataagt
     1441 atggttcttg aacaagtctt cccatacctg cggacacact tcgttactga ttgctgtgcg
     1501 aagaagtcca gcaactgtaa aaccagcctt cttttcaata cgaacatcca tttctttacc
     1561 tccttggatg gttaatgata gttgcaaggg taagaggacc ttaaaaggca accccttacg
     1621 cacttgacta ggagaaatct gatgaaattg tttgaaggca aaggcaaaag aatccgaaga
     1681 ttcataccca tatttaaagg ctatatctat aatctttgcc tgtccttcac gaatgtccat
     1741 agcagctcgc gtcaactttc gtaaccgaac gtattctgac aaggacatat ctgtcagaat
     1801 cgaaaagaga cgactaaaca tgggaacgga atatcctgat atgcgcaaaa attcccgttc
     1861 atcgatttcg ctttctaaaa cggactctag gtaacgtacg gttgcattga actcttttat
     1921 catcaacttc ccctttccct atcagtttac cgtctaccat ccaagttttc ccgatacttt
     1981 tcgtcggaaa actatcggct taaaccgtca aaaactcttc caagtccttg agggaacgct
     2041 ctgctattgc ttcataacgc tccttcttat cgcggatacg tggtccatcc agctccttga
     2101 tgatgccaaa gttgacattc ataggctgga agtgcttgct ttctgtgtgg gtgatgtaga
     2161 atggcagagc accgatggct gtggtctgtg ggaagatgac cggctcttcg cctcggaagc
     2221 gacgaacagc attgataccg gccactaagc cagaagcggc agactcaaca tagccttcta
     2281 ctcctgtcat ttgacctgca aagaaaagat ttggattttt cttggtcgca aaggtctgtt
     2341 ccaataggtt tggcgaatcc atgtaggaat tgcggtgcat gacaccataa cggacaaatt
     2401 cagcattttc aaggcctgga atcatctgga agacccgctt ctgctcgccc cacttaaggt
     2461 gagtctggaa gccaacaata ttgtaaaggc tacctgctgc gttgtcctga cggagttgga
     2521 caacagcata gggtgtcttg tattcaccgt cacgaggtcc cttgtagtct tctgggtatt
     2581 ccagaccgac tggcttcatc ggaccataca acatggtttt gatcccacgt ttggccatga
     2641 cttcaatggg catacagcct tcaaaatatt tttctttttc aaaggaattg agcggtgctt
     2701 cctcggctga aatcaaggcc tcatagaaag cggtaaactg ctccttgttc atcggagcat
     2761 tgagataggc agcctctccc ttatcataac gggacttgag ataaaccaag tccatgttaa
     2821 tggtcgaact atcgacaatc ggtgctgccg catcgtagaa gtaaaaaccg tcgccaccat
     2881 tgagtgcgtg aatcttctcc gccaaggcgt cagatgtcaa aggccccgtc gcgataactg
     2941 taatagcatc gtcaggaatg tccgtaatct caccgcgaat gacctcaatc agtgggtgat
     3001 tgtgaatctc atctgtgacc gcttgggaga aattctcacg gtccatggcc atagcaccgc
     3061 cagcaggcac acggtgagcc tcaccagctc gcatgataat ggaatccaag cgacgcattt
     3121 cttccttgag aagaccgaca gcgttggtca aactatcacc acggaatgaa ttggaacaaa
     3181 ccaattccgc aaacttgtcc gttttgtgct gaggagtcgg cttgacacca cgcatctcgt
     3241 aaagtttgac aggaatgcca cgtttggcaa tctgataagc agcttctgag ccagccaagc
     3301 cagctccgat aacgttaatg tgggtttgag acatgaaact aatacctcta gtcagaatat
     3361 ctgactactg tttgggaata ccccaaaaca gaacctttct aaattttata cctatctagt
     3421 ataccagaaa
//
LOCUS       DBNFEG010000181         3407 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.181, whole genome shotgun sequence.
ACCESSION   DBNFEG010000181 DBNFEG010000000
VERSION     DBNFEG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.181"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..465
                     /locus_tag="AC1PNG_001888"
     CDS             <1..465
                     /locus_tag="AC1PNG_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HIP6702368.1"
                     /translation="RYHWQPDLSWAIIYWSLTFTPIFIGMTLLLEKLQVSRLFILLVS
                     LFAVLVVLGLHRYFEIKDEHLRIASANPFAVQKIEIATIEKIEVSYLAIRIFSQEFPN
                     GQVYHMRKWTKKYFINHLAIHSCFKGKIELIDHLIKQDYFEEYYTKKAKSIR"
     gene            complement(454..648)
                     /locus_tag="AC1PNG_001889"
     CDS             complement(454..648)
                     /locus_tag="AC1PNG_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIP6702369.1"
                     /translation="MKVKLIPERCIACGLCQTYSSLFDYDDDGIVVFSDESQFEKTIE
                     PNPDILKAIKSCPTKAISAD"
     gene            699..1175
                     /locus_tag="AC1PNG_001890"
     CDS             699..1175
                     /locus_tag="AC1PNG_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HIP6702370.1"
                     /translation="MSQEPWNEEVYHTETTSRKERLSKGVADTTVFTVLAIIFFVIVL
                     IIGIMAVYLSIGGGSKTDSTKEFYTPSTTTVVAESSSSAAETQESTSTDTTETETSSS
                     PAEATDGSTLTVQAGEGVGQLAARGGISIAELERLNPEKMTTGSWIAHPGDVVRIR"
     gene            1185..1862
                     /gene="cmk"
                     /locus_tag="AC1PNG_001891"
     CDS             1185..1862
                     /gene="cmk"
                     /locus_tag="AC1PNG_001891"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIP6702371.1"
                     /translation="MKSIQIAIDGPASSGKSTVAKIIAKNLGYTYLDTGAMYRSATYL
                     ALTNGIEVTDQNRIVDLLAQYPIRFGRDENGQQLVFVGDEDVTLPIRDNQVTNNVSAV
                     AALPLVREELVRLQQDIAQAGGIVMDGRDIGTVVLPQAELKIFLVASVEERALRRFKE
                     NTERGIETDLESLKEEIAARDYKDSNREVSPLKAADDAITFDTTGVSIEGVVKFISEK
                     AKEILDR"
     misc_feature    1889..2017
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2058..2564
                     /gene="infC"
                     /locus_tag="AC1PNG_001892"
     CDS             2058..2564
                     /gene="infC"
                     /locus_tag="AC1PNG_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIP6702372.1"
                     /translation="MFINDEIRVREVRLIGLEGEQLGIKPLNEAQAIADSANVDLVLI
                     QPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGDFETKLRHA
                     RKFLEKGNKVKVSIRFKGRMITHKEVGAKVLAEFAEATQDIAIIEQRAKMDGRQMFMQ
                     LAPASDKK"
     gene            2591..2791
                     /gene="rpmI"
                     /locus_tag="AC1PNG_001893"
     CDS             2591..2791
                     /gene="rpmI"
                     /locus_tag="AC1PNG_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIP6702373.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKAGMVHAGDFKRIKSMLTGLK"
     gene            2835..3194
                     /gene="rplT"
                     /locus_tag="AC1PNG_001894"
     CDS             2835..3194
                     /gene="rplT"
                     /locus_tag="AC1PNG_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIP6702374.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHLLFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AAAFTALADAAKAKLAK"
BASE COUNT         1076 a          623 c          729 g          979 t
ORIGIN      
        1 cgttaccatt ggcaaccgga tttatcatgg gccatcatat actggtcact taccttcaca
       61 cctattttta ttggtatgac acttttattg gagaagttgc aagtttcaag attatttatt
      121 ctacttgtct cactctttgc tgtattagta gtgctcggct tacatcgcta tttcgagata
      181 aaagatgagc atttacggat tgcttcggct aatccttttg cagtacagaa aattgaaatt
      241 gctaccatag aaaaaattga agtttcttat ctagccattc gcatattttc acaagaattt
      301 cccaatgggc aggtgtatca catgcgtaaa tggacaaaaa aatactttat taaccattta
      361 gctattcata gttgcttcaa gggtaaaatt gaattaatag accatttaat aaaacaggat
      421 tattttgaag agtattatac aaaaaaagcc aaatcaatcc gctgaaatgg cttttgtagg
      481 acatgatttg atggctttaa gaatatctgg attgggttca atcgttttct caaattgtga
      541 ttcgtctgaa aaaacaacga taccgtcgtc atcataatcg aatagagatg agtaggtttg
      601 gcagagcccg caagcaatgc accgttcagg aataagtttt actttcatat tagtattgta
      661 atcatatttt aataaaaata caaggagttt tctcaataat gtcacaagag ccatggaatg
      721 aagaagttta ccacacagaa acaactagtc gtaaagaacg tctgagtaag ggggtagcgg
      781 atacaacagt atttacagtt ttggcaatta ttttctttgt tatcgtactg attattggaa
      841 ttatggctgt ctatctctca atcggtggcg gtagtaaaac agattcaaca aaagaatttt
      901 ataccccaag cactacaaca gtcgtagcag agagctcttc atcggctgct gagacacaag
      961 aatctacttc gactgacacg acagaaacag aaacaagttc tagcccagcg gaagcaacgg
     1021 atggctctac ccttactgtt caagcaggtg aaggtgtagg acaactggct gctcgtggtg
     1081 ggatttcaat tgcagagcta gagcgtttga atccagagaa aatgactaca gggtcttgga
     1141 ttgcgcatcc tggggatgta gttcgtatta gataaagagg agttatgaaa tcaattcaaa
     1201 ttgcaattga cggtccagct tcaagtggaa agtcaacggt tgctaaaatt attgctaaaa
     1261 atttgggata tacttactta gatacaggag ctatgtatcg ctcggctacc tatctagcct
     1321 tgacaaacgg catagaggtc acagatcaaa atagaatcgt ggacttgctt gcccagtatc
     1381 ccattcgatt tgggcgtgat gaaaacggtc agcaacttgt ttttgtcggt gatgaggatg
     1441 ttactttgcc tattcgagat aatcaagtaa caaataatgt atcagcggta gcagcgcttc
     1501 ctctagtacg ggaagaattg gtccgcctgc aacaggacat tgcgcaagct ggtgggattg
     1561 ttatggatgg tcgtgatatt ggtacggttg tattgccgca agcagaactc aaaatattcc
     1621 ttgttgcctc tgttgaagag cgtgcccttc gtcgatttaa ggaaaatacg gaacgtggca
     1681 ttgagacaga cttggaatcc ttgaaagagg agattgcggc tcgtgactat aaagattcca
     1741 atcgagaagt ttctccttta aaggcagcag atgatgctat tacctttgat acaacaggag
     1801 tatctatcga aggtgtagtg aaatttattt ccgaaaaagc gaaagaaatt cttgacaggt
     1861 agctttagac atgttagaat agtcgtattg agaaaagcag aagtgagaac ttctcgcctt
     1921 gcgactaacg ttgtctggct ctacggatta agatttcttc ggaaagaata atggtgtagt
     1981 gcggacttgt ttagcaagtc cgtttcgttt ttctcgaaaa atataaaatg aggtgaaaag
     2041 ccatagcaaa gcaagatttg ttcatcaatg atgaaatccg agtgcgtgaa gttcgtctta
     2101 tcggtcttga gggtgaacag ttgggaatca aaccactcaa tgaggctcag gccattgctg
     2161 atagcgccaa cgtggattta gtattgattc aaccccaagc taaaccacca gtagcgaaaa
     2221 ttatggacta cggtaagttc aaatttgaat atcagaagaa acagaaagaa caacgcaaaa
     2281 aacaaagtgt tgtcactgta aaagaagtgc gtctaagtcc agttattgat aaaggggatt
     2341 tcgagacaaa acttcgtcat gcccgtaaat ttcttgaaaa aggaaataaa gttaaggttt
     2401 cgatccgttt taagggtcgt atgattaccc acaaagaagt tggagcgaaa gtattagctg
     2461 agttcgctga agcaacccaa gatattgcca ttatcgaaca acgcgctaag atggacggac
     2521 gtcaaatgtt tatgcaattg gccccagctt cagataaaaa ataagctttt taagcaaaag
     2581 gagaattaac atgccaaaac aaaaaactca ccgcgcatca gctaaacgtt tcaaacgtac
     2641 aggttctggt ggattgaaac gcttccgcgc ttatacttca caccgtttcc acggtaaaac
     2701 taaaaaacaa cgtcgtcacc ttcgtaaagc aggtatggta catgctggtg acttcaagcg
     2761 tatcaaatca atgcttacag gtttgaaata agccgactta ctagaattta gattatcatt
     2821 tggaggaaaa ttaaatggca cgtgttaaag gtggcgttgt ttctcgtaaa cgccgtaaac
     2881 gtattttaaa attagctaaa ggttactacg gagctaaaca tctcttgttc cgcactgcga
     2941 aagaacaagt catgaactct tactactatg cataccgtga ccgccgtcag aaaaaacgtg
     3001 atttccgtaa attgtggatc actcgtatca atgcggctgc tcgtatgaac ggtttgtcat
     3061 actcacaatt gatgcacggt ttgaaattgg ccgaaatcga agtaaaccgc aaaatgcttg
     3121 ctgacttggc agtgaacgat gcagcagctt tcacagctct tgcagatgct gctaaagcaa
     3181 aacttgctaa ataagtataa aaatgtaaag aacttggtag tgatgaacca ggttctttat
     3241 ttttggaaag tagctcacta cttgctaaat ttcgatatga gtgatatact taactagtaa
     3301 ataaatcgac ggttttaact ttatcgtcat ttagtttttc ttttgttact tcatcaactc
     3361 gccttgccta acttcagtta tgcctgcggc tcattgcctc gtactaa
//
LOCUS       DBNFEG010000182         3358 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.182, whole genome shotgun sequence.
ACCESSION   DBNFEG010000182 DBNFEG010000000
VERSION     DBNFEG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.182"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..921
                     /locus_tag="AC1PNG_001895"
     CDS             <1..921
                     /locus_tag="AC1PNG_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HIP6702375.1"
                     /translation="FTLNNWGINKYQRHYACSEIKVYFNEDRTSMGVFIELKGQGCRQ
                     YEEFLDGNENNWIALIGRLYQYSVNFTRLDIAHDIFDGSLDVQRVYDYCKKGLCISKA
                     KHFEYHEKSVLENGERVGETVAIGSRGNQQWCIYNKRMEQLSKQELVEYPSWIRAELR
                     CWQDKANIIAEQLFLKRPLSSIYFEAINGHYRFVKPNATDTNRWRRKKVKWWLDYLKT
                     ENQTVLSVERTKPTLRQSEAWTEKQVAKTLAKVYTAKYQAYDVQKAEDYIQGLLKEGL
                     SRLTDNDEKDIEQYIREQNSSPLWGQKKTT"
     gene            982..1188
                     /locus_tag="AC1PNG_001896"
     CDS             982..1188
                     /locus_tag="AC1PNG_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HIP6702376.1"
                     /translation="MIATVNKNDLVALGFSEGTSKRIIRQGKELLIARGFRVYQNKRV
                     GTIPASIATELLGFDVSLGAHHDS"
     gene            1178..2311
                     /locus_tag="AC1PNG_001897"
     CDS             1178..2311
                     /locus_tag="AC1PNG_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIP6702377.1"
                     /translation="MTVKKAKNGTWTVDISDGFHPVTKKRIRIIRKGFKTKKEALELE
                     QYIRVVELKEKRFDFLVTTDMLFDILEEEDRQNNRKISYISTQRNNYERHIKPYFINT
                     NLNKLSYEHIFEFREYLKIKPKKQNDSSTLSHNTVNKIMILLKKIFDTGVRKSLIDKN
                     PVENIRKLPISKPTINFWNVEEFNEFRKLITSDEISYDLFFTIAFFTGMRLGEIVALT
                     WYDINLITNSIHITKTAYFVNGTNHINSTKTRAGTRRITINHKLARMLADWKEKQKDL
                     LKEFTTDTENLQVIQSTPLPMTKNMIDKKFKQILARNDYLKKIRIHDLRHSHASLLIN
                     QGEDYLVVKERLGHASITTTIDTYSHLYPSKQKTLANKLDDIF"
     gene            complement(2398..2481)
                     /locus_tag="AC1PNG_001898"
     tRNA            complement(2398..2481)
                     /locus_tag="AC1PNG_001898"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2445..2447),aa:Leu,seq:caa)
     gene            complement(2493..2564)
                     /locus_tag="AC1PNG_001899"
     tRNA            complement(2493..2564)
                     /locus_tag="AC1PNG_001899"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2530..2532),aa:Gln,seq:ttg)
     gene            complement(2576..2648)
                     /locus_tag="AC1PNG_001900"
     tRNA            complement(2576..2648)
                     /locus_tag="AC1PNG_001900"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2613..2615),aa:His,seq:gtg)
     gene            complement(2656..2726)
                     /locus_tag="AC1PNG_001901"
     tRNA            complement(2656..2726)
                     /locus_tag="AC1PNG_001901"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2692..2694),aa:Trp,seq:cca)
     gene            complement(2734..2814)
                     /locus_tag="AC1PNG_001902"
     tRNA            complement(2734..2814)
                     /locus_tag="AC1PNG_001902"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2778..2780),aa:Tyr,seq:gta)
     gene            complement(2835..2907)
                     /locus_tag="AC1PNG_001903"
     tRNA            complement(2835..2907)
                     /locus_tag="AC1PNG_001903"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2872..2874),aa:Phe,seq:gaa)
     gene            complement(2923..2996)
                     /locus_tag="AC1PNG_001904"
     tRNA            complement(2923..2996)
                     /locus_tag="AC1PNG_001904"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2960..2962),aa:Met,seq:cat)
     gene            complement(3006..3095)
                     /locus_tag="AC1PNG_001905"
     tRNA            complement(3006..3095)
                     /locus_tag="AC1PNG_001905"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3057..3059),aa:Ser,seq:tga)
     gene            complement(3104..3175)
                     /locus_tag="AC1PNG_001906"
     tRNA            complement(3104..3175)
                     /locus_tag="AC1PNG_001906"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3140..3142),aa:Glu,seq:ttc)
     gene            complement(3182..3255)
                     /locus_tag="AC1PNG_001907"
     tRNA            complement(3182..3255)
                     /locus_tag="AC1PNG_001907"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3219..3221),aa:Ile,seq:gat)
     gene            complement(3286..3356)
                     /locus_tag="AC1PNG_001908"
     tRNA            complement(3286..3356)
                     /locus_tag="AC1PNG_001908"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3322..3324),aa:Gly,seq:tcc)
BASE COUNT         1158 a          645 c          694 g          861 t
ORIGIN      
        1 ttcacattaa ataattgggg aataaacaag taccagcgac attatgcttg ttctgagatt
       61 aaagtgtact ttaatgagga tagaacttca atgggggttt ttatcgaatt aaaagggcaa
      121 ggttgtcgcc aatatgaaga atttttggac ggtaacgaaa ataattggat agctttgata
      181 ggtcggttgt atcagtattc ggttaatttt actcgcttag atattgctca cgacatattt
      241 gacggtagtt tagatgttca gcgagtatat gactattgca agaaaggttt atgtatctcg
      301 aaagcaaagc attttgagta tcatgagaaa tctgtcttag aaaatggaga acgagttgga
      361 gagacggttg ctataggttc cagaggtaat caacaatggt gtatctacaa taaacgtatg
      421 gaacaactta gcaagcaaga gcttgtagaa tatccctcat ggattcgtgc ggaacttaga
      481 tgttggcagg ataaggcgaa tattattgca gagcaattgt ttctaaagcg tcctctgtca
      541 tctatctatt ttgaagctat caatggtcac tatcgttttg tgaaaccaaa tgcgacagat
      601 actaatcgtt ggagaagaaa aaaggtcaag tggtggctag actatctaaa gaccgaaaat
      661 cagactgttt tgagcgttga gagaactaag cctacattac ggcagtcaga ggcttggaca
      721 gagaaacaag ttgcgaagac attggcaaaa gtctatacag caaaatatca agcctatgat
      781 gttcaaaagg cagaggacta cattcaaggt ctactaaaag agggcttgtc aaggctaact
      841 gataatgatg aaaaggatat agagcaatat attcgagagc agaatagttc tcccttatgg
      901 ggacaaaaaa agacgacttg aaaacaagtc gcctctaaaa attaactaac tagattataa
      961 caaaagaaag gaaagtgtcc aatgatagca accgtaaata agaatgacct agttgccctt
     1021 ggtttctctg aaggaacttc taaacgtatc atcagacagg ggaaagagct tctaatagca
     1081 agaggattta gggtttatca aaataagcgt gtcggaacta tccctgcttc tatcgctact
     1141 gaattacttg gttttgacgt ttctttaggt gctcatcatg acagttaaga aagcaaaaaa
     1201 tggaacttgg actgttgata tttctgacgg cttccaccca gttaccaaaa agcggatacg
     1261 aatcattcgt aaaggtttca agacaaagaa agaagcttta gagttagaac aatatatccg
     1321 tgttgttgag ttaaaagaaa aacgttttga ttttttggta acaaccgata tgctcttcga
     1381 tatacttgag gaagaagacc gacaaaataa cagaaaaatc agttatatca gcacacaaag
     1441 aaacaactat gagcgacata tcaaacctta cttcataaat acaaacttaa ataaattatc
     1501 ttatgagcac atttttgaat ttcgagagta tctcaaaata aagcccaaaa agcaaaacga
     1561 tagctctacg ttaagtcata atacagtcaa taaaataatg atactcctca aaaagatttt
     1621 tgatactggt gtaagaaagt cattgataga taaaaatcca gttgaaaata taagaaaatt
     1681 gcctattagt aagccaacta taaatttttg gaatgtagaa gaatttaacg aatttagaaa
     1741 acttattaca agtgatgaaa taagttacga cctattcttt acgattgcct tttttactgg
     1801 catgcgattg ggcgagatag ttgcgttaac ttggtacgat ataaacttaa tcactaatag
     1861 cattcacatc acaaaaacag catattttgt taacggtaca aatcatatca atagtacaaa
     1921 aactagagca ggtaccagac gtattacaat caatcataaa ttagcaagaa tgcttgcaga
     1981 ctggaaagaa aaacaaaaag acttacttaa agaatttaca acagatacag aaaaccttca
     2041 agtgattcaa agcacaccac taccaatgac aaaaaatatg attgataaaa aatttaagca
     2101 gatactggct aggaacgact atctaaaaaa gatacgaata cacgatttaa ggcactctca
     2161 cgcgtcttta cttatcaatc aaggggaaga ttatctagtc gtaaaagaac gtcttggaca
     2221 cgcctctatc acgactacaa tagatacata ttcccactta taccctagta aacaaaagac
     2281 attggcgaac aaattggacg atatttttta gaaaatggaa ttttttggca acttgaaaaa
     2341 gagcaataga aaagataaaa caaaagacaa ggcttagaaa ccttgtcttt ataactatac
     2401 cggcggccgg ggtcgaaccg gcacgtcctt gcggacactg gattttgagt ccagcgcgtc
     2461 tgccaattcc gccacgccgg catatagttg aactggggta gctggattcg aaccaacgca
     2521 tgagggagtc aaagtccctt gccttaccgc ttggcgatac cccaatatta aaataggcga
     2581 gtgaggggaa tcgaaccccc gaatgtcaga gccacaatct gatgtgttaa ccacttcacc
     2641 acacccgcca tataacacgg gcagtaggaa ttgaacccac actgaaggtt ttggagacct
     2701 tagttctacc tttaaactat gcccgtaaag gtatggagag agagggattc gaacccccga
     2761 acccgaagga gcggatttac agtccgccgc gtttagcctc ttcgctatct ctccgaaata
     2821 taatattaaa ttaatggcgc gagacggaat cgaaccgccg acacatggag cttcaatcca
     2881 ttgctctacc aactgagcta ccgagccaaa ttcttaaaga aattgcggga gcaggatttg
     2941 aacctacgac cttcgggtta tgagcccgac gagctaccta gctgctccat cccgcgataa
     3001 tagtaaggag gatgtgggat tcgaacccac gcacgctttt acacgcctga cggttttcaa
     3061 gaccgttccc ttcagccgga cttgggtaat cctccgaata atatagtccg tacgggattc
     3121 gaacccgtgt taccgccgtg aaaaggcggt gtcttaaccc cttgaccaac ggaccatata
     3181 atgggcacga gtggaatcga accaccgacc tcacgcttat caggcgtgcg ctctaaccat
     3241 ctgagctacg cgcccaaacc aagtttgggt atgaactatg ttcaaagcgg gtgacgagaa
     3301 tcgaactcgc gacaacagct tggaaggctg tagttttacc actaaactac acccgctt
//
LOCUS       DBNFEG010000183         3350 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.183, whole genome shotgun sequence.
ACCESSION   DBNFEG010000183 DBNFEG010000000
VERSION     DBNFEG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.183"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..594
                     /locus_tag="AC1PNG_001909"
     CDS             <1..594
                     /locus_tag="AC1PNG_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIP6702378.1"
                     /translation="NLYQAQTQLNTTRSAAENAKATSFEIQKGVIIFGDSVTVRASSA
                     IQTALPDAQIDGTVSRNLSEIAKLINLYKQNNSLKETVVVALGTNTTDNYQELLDQLV
                     KDFPKGRRLIFVTPYDGNFTPSESISYQTGQYEKELAEKYDYISIADWYQVAKDNPPI
                     WYNTDLVHFNLESNGGEVFAQTIKNAVEAVADGPIKN"
     gene            complement(818..1471)
                     /locus_tag="AC1PNG_001910"
     CDS             complement(818..1471)
                     /locus_tag="AC1PNG_001910"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIP6702379.1"
                     /translation="MKNKQIHLRNASFFALAFVILGYVVKFYPSTLAGFDSTVQSAIR
                     GNLPSGASQFWTSITVLGNTVIILAITLILAAIFYYYKKWKIEAYFLLASFAAMGVAS
                     TALKYVYQRPRPSIEWLIDTIGYSFPSWHTASTMMIVGAVVIIVHQRMKKGIARLVLK
                     AVLILLAVLVAVSRIYIGVHYPTDIIGGWLLAVTLLQMMYPFYDQKRFEWRFQSKQK"
     gene            complement(1496..2053)
                     /locus_tag="AC1PNG_001911"
     CDS             complement(1496..2053)
                     /locus_tag="AC1PNG_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIP6702380.1"
                     /translation="MTNTRKMTIIAILSAVSFLLMYLKFPLIPTASFLEVDFSLVPIL
                     FGLLVLDMKSSFAILLVRTFLKLILNNQGPSTIIGLPMNIAAMSVFILAVAYFWKNEQ
                     TLKNYLKAAAVATIGSTAVMLVLNYVYAVPVYAAFANFDIKEILGLTNYLLMMVLPFN
                     LLQGIVLSAIFYICYKAAQPILKKV"
     regulatory      complement(2113..2327)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(2377..2922)
                     /locus_tag="AC1PNG_001912"
     CDS             complement(2377..2922)
                     /locus_tag="AC1PNG_001912"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0141098 - tRNA
                     (cytidine(34)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HIP6702381.1"
                     /translation="MNIETLEYIEETAKNHIVLFEPQIPQNTGNIARTCAATNSPLHI
                     IKPMGFPIDDRKMKRAGLDYWDKLDVRFYDNLAEFMDYASKQGRVHLVSKFADKTYSD
                     EDYNDGNIHYFLFGREDKGLPEDFMREHPEKAIRIPMNDEHVRSLNVSNTACMIVYEA
                     LRQQQFAGLDLVHTYDGDKLK"
BASE COUNT         1087 a          686 c          614 g          963 t
ORIGIN      
        1 aatctttatc aagctcagac ccaactaaat acaacacgat ctgctgctga aaacgctaaa
       61 gctactagtt ttgaaattca aaaaggagtt atcatctttg gcgattctgt tactgttcgc
      121 gctagttctg ccatacagac cgcgctacca gatgcacaga tcgatggaac cgtcagtcga
      181 aatctctctg aaattgctaa attgataaat ctttataagc aaaataatag cttgaaagag
      241 actgtagttg tagccctagg tacaaatact acggataatt atcaggaact actagaccag
      301 ctagtcaaag attttcctaa aggacgtcgc cttatttttg tcactcctta cgatggaaac
      361 ttcactccga gcgaatccat ttcctatcaa actggtcaat acgagaaaga attggctgaa
      421 aaatatgatt acatttccat tgcagactgg tatcaagttg caaaggataa tccaccaatt
      481 tggtacaata ccgaccttgt tcatttcaat cttgaaagta atggaggcga agtcttcgcc
      541 caaacgataa aaaatgctgt tgaagccgtt gctgatggtc caatcaaaaa ctagatttat
      601 acatcattat cctctgagaa tttctcggag gatttttgca ctattttcct ataagtccag
      661 cgcgaactct aagaaatggc gttgtgcctg agctcagcct caataaatct tgtgaaataa
      721 gccacttcta caaaggacgg caaagcgaag taacgataat agaaagatga caagttaaaa
      781 ctcctacctt ttacagtagg agttggtgtc aaattgacta tttttgctta ctttggaaac
      841 gccattcaaa gcgtttctga tcgtaaaatg gatacatcat ttgtaaaaga gtgacggcaa
      901 gtagccaacc accgataata tctgtcgggt agtgaacccc aatatagata cgggatacag
      961 ctaccaggac agcaagtaga atcaacacgg cttttagcac aagtctagct atgcctttct
     1021 tcatcctctg gtgaacaata ataacaactg ctcctacaat catcatagtt gatgcagtat
     1081 gccagctagg aaatgaataa ccgatggtat caatcaacca ttcaatactt ggacgcggac
     1141 gttggtagac atatttcagg gctgtggatg ccacacccat cgccgcaaaa ctagcaagta
     1201 ggaaataagc ttctatcttc cattttttat agtagtaaaa gatagcagct agaatcaagg
     1261 taatagctaa aataataacc gtatttccta aaacagtaat agaggtccaa aattgactgg
     1321 ctccacttgg taaatttcca cgaatggcag actggacagt cgagtcaaaa cctgctaggg
     1381 tactaggata aaattttact acatagccca atatcacaaa ggccagagca aaaaaagatg
     1441 cgtttcgtaa atggatttgt ttattcttca tacagtatag tttaccttat aaaggttaaa
     1501 ctttctttaa aataggttga gctgctttgt aacagatata aaatattgct gaaagcacaa
     1561 tcccttgtaa aagattaaaa ggaagtacca tcatcaaaag atagttagtc agccccaaga
     1621 tttctttaat atcaaaattt gcaaaggctg cataaacagg caccgcataa acgtaattta
     1681 ataccagcat cactgctgtc gaaccaattg tcgcaacagc ggctgctttt aaataatttt
     1741 ttaacgtttg ttcatttttc cagaaataag ctactgctaa aatgaaaact gacatagctg
     1801 caatattcat cggtaagcca ataattgtcg atggtccctg attatttaaa atcagtttga
     1861 gaaaagttcg taccaaaagg atagcaaaac tagacttcat atctagaaca agcagaccaa
     1921 ataaaatcgg tacgagagaa aaatccactt ccaggaaact ggctgttgga atcagcggaa
     1981 atttcaaata catgagcaga aaggatactg ctgagagaat ggcgataatc gtcattttgc
     2041 gtgtgtttgt cataaggttc ctccaatttt tgaaaattga agaagcgtgg aagagcaggc
     2101 aaaaagcctc cacttcgtct tctcccatcc agactttact gtcggttgtg gaattgcacc
     2161 acatcagctt tcgctcgcag actaccaacg gtctattttg ttctaatact gcgttagctc
     2221 atctcgacat acattcagta tggtcttcga ttcgctgcct agtcttaaaa caaattacca
     2281 cgttttacac tgccggtcgg gaatttcacc ctgccctgaa gactatttca tcataccaaa
     2341 aaagccttgc atttgcaaga gcttggtaag ttttcactat tttaacttgt ccccatcata
     2401 ggtgtgaacc aaatctagac cagcgaactg ttgctgccga agagcttcat atacaatcat
     2461 acacgctgta ttagagacat taagactacg cacatgttca tcattcattg gaatacggat
     2521 agccttctct ggatgttcac gcataaaatc ttctggcaag cccttatctt cacgaccaaa
     2581 taagaaataa tgaatgttcc catcattata gtcttcatcc gaataggttt tatcagcaaa
     2641 tttagatact aagtgaacac gtccttgttt gctggcataa tccataaatt ctgctaaatt
     2701 gtcatagaag cgaacatcta acttatccca ataatctagt cctgcacgtt tcatcttacg
     2761 gtcatcaatt ggaaatccca taggcttaat gatatggaga ggactattgg ttgcagcaca
     2821 agttcgagca atattgcctg tattctgtgg aatctgtggt tcaaataaga caatgtggtt
     2881 tttggctgtt tcttctatat actctaatgt ttcaatattc ataaggaccc ctctggtgac
     2941 atttcactaa acacgaatac ccttcgaaaa tcaaatccaa accatgtcag atgcatttta
     3001 ccgtatacca gtacggtctg ttgcttgtta tctagtttct ttttttgatt ttcattgagt
     3061 aaaaagactc gactgtctcc tatggcaaga aagacaaaaa aatagccaca ctgcccggag
     3121 tcaagctcag caaacagcat ggtgaatgat gattcattca cttaactcac aacaggtttg
     3181 agttaaatca atgaaatcgt gatactattg tagcttaaag aaggctgaaa atcaagagtt
     3241 ttgccctatt tttttcaaat tttcatagaa aatcatgggg gctgggcaaa aagaccaacc
     3301 tagcttcgca gagttcgtgt caacatctga gcgcagtgat tgattggctt
//
LOCUS       DBNFEG010000184         3206 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.184, whole genome shotgun sequence.
ACCESSION   DBNFEG010000184 DBNFEG010000000
VERSION     DBNFEG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.184"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            63..356
                     /locus_tag="AC1PNG_001913"
     CDS             63..356
                     /locus_tag="AC1PNG_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HIP6702382.1"
                     /translation="MQKTLEKLKRSELFELLVEQAKEIESYQAKLVDLEKRIEYREAV
                     LGEIGSIAEASLAVTRIFEEAQLAANIYLEQVQKVTHKQSDNCEIGRTDEDGD"
     gene            346..948
                     /gene="lepB"
                     /locus_tag="AC1PNG_001914"
     CDS             346..948
                     /gene="lepB"
                     /locus_tag="AC1PNG_001914"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIP6702383.1"
                     /translation="MATNKNKDYVTISDLPSISIMSEELDKIRYKKRYLETLKNTIFT
                     LVTVVALAVLIAMLWLPVLHIYGSSMSPTLKAGDMVATVKTNTLSTGDMVAFYYNNKV
                     LVKRVVATSGQWVNIDEQGNVYANGKTLDEPYVKDKAYGQTDIKLPYQVPEGQYFVMG
                     DHRSVSIDSRNTAIGSIGEEQLVGKLTFRIWPFHKMGVIK"
     gene            967..1353
                     /locus_tag="AC1PNG_001915"
     CDS             967..1353
                     /locus_tag="AC1PNG_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702384.1"
                     /translation="MKILWERLFKFVQQCFREKKTAKITMALSIIMAFGISYLLILPA
                     ITLETDSQESVLQSVYNQPTTVEQPVQEEQAESQLAQVQSAGSLFAEHENITVQVNYE
                     EHTFEQPVRLQVLPSEDSTKYIGLVR"
     gene            complement(1354..1743)
                     /locus_tag="AC1PNG_001916"
                     /pseudo
     CDS             complement(1354..1743)
                     /locus_tag="AC1PNG_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1711..2538
                     /locus_tag="AC1PNG_001917"
     CDS             1711..2538
                     /locus_tag="AC1PNG_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIP6702385.1"
                     /translation="MASQIRHDLSLLVFFLITGLLLLLYPTVSDYWNSHYQSEAVATY
                     SEKLLDISKEEKQKILENVSAYNSTLEKGTIPNLNIDSVALKKYDSILNITDTGIMGY
                     VEIPKLQTMLPIYHGTDDAVLQVAVGHLAGTSLPIGGQGSHAAISGHRGLPSAKLFSD
                     LDQLVIGDTFMIQVLDNTLTYEIDKIITVTPDDVSPLMIDPNEDFVTLVTCTPYGVNS
                     HRLLVRGHRIENQLLARQVVSEGSIVKPILVSPFIALCLLGLLWSLFFVKKEVNLFM"
     gene            2669..2845
                     /locus_tag="AC1PNG_001918"
                     /pseudo
     CDS             2669..2845
                     /locus_tag="AC1PNG_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104139.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            2945..>3206
                     /locus_tag="AC1PNG_001919"
     CDS             2945..>3206
                     /locus_tag="AC1PNG_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIP6702386.1"
                     /translation="MNIKEVSDVTGLSADTIRYYERIGLIPKIARKSSGVRNFSESDV
                     AILEFIRCFRSAGMSIERLIEYMGLVQAGDSTVEARIDLLKEE"
BASE COUNT         1077 a          501 c          682 g          946 t
ORIGIN      
        1 aagcatatat tttgtagcga aagcaatata agcaaaaagg agagaggaag aagcaatgtt
       61 aaatgcaaaa gaccttagaa aaactgaagc gttctgaatt atttgaactc ttggttgagc
      121 aggcaaagga aatagagtct tatcaagcaa aattagtaga tttggaaaaa agaattgagt
      181 atcgcgaagc ggttttggga gaaattggtt cgatagcgga ggcgtctcta gcagttacta
      241 ggatttttga agaagcacaa ttggctgcaa atatttatct tgaacaagtt caaaaagtta
      301 cacataaaca atctgataat tgtgaaatag ggagaacaga tgaggatggc gactaataaa
      361 aataaggatt atgttaccat atcagattta cccagtatat caataatgtc agaggagctg
      421 gataaaatac gatataaaaa gcggtattta gagacattaa aaaatactat tttcacgctt
      481 gttactgtcg ttgcacttgc agtattaata gccatgttat ggttgccagt tctacatatt
      541 tatggaagtt ccatgtctcc tactctgaaa gcaggtgata tggtggcaac tgtgaagaca
      601 aatacacttt caactggaga tatggttgca ttctactaca ataataaggt tttggtaaaa
      661 agagtagtag caacttctgg tcagtgggta aatatagatg agcaggggaa tgtttatgca
      721 aatggaaaaa cattagacga accctatgtt aaagataagg cctatggtca aacggatatt
      781 aaactaccgt accaggttcc agaaggtcaa tattttgtaa tgggagatca tcgcagtgta
      841 tctattgatt ctagaaatac agctattggg tcgattggtg aggagcaact ggttggaaag
      901 ttaacctttc ggatatggcc atttcataaa atgggagtaa tcaaataaag gaaggagata
      961 taaaacatga aaatattatg ggaaagattg tttaaatttg ttcagcaatg ctttcgagag
     1021 aaaaagactg caaaaataac tatggcgttg tcaataatta tggcttttgg aatatcatat
     1081 ttgctgattt taccagccat tactttagag acagacagtc aggagagtgt tctgcaatca
     1141 gtctataatc agccaacaac agtagaacag ccagttcaag aagaacaggc ggagtcacag
     1201 ttagcacagg ttcaatctgc tggaagctta ttcgctgaac atgaaaatat aactgtacaa
     1261 gttaactatg aggagcatac atttgaacag ccagttcggt tacaggtttt accctcagaa
     1321 gatagtacaa aatatattgg acttgttcgg taactacatc atctcatggt tgatgatacc
     1381 acaaatcaac tcagttctta attcaaaccg ttttctacga ttacgatagg gctgtgccat
     1441 gattttaaag gttttaaatt tggcattaaa gtgttcaact tcaatacgga ttttagctat
     1501 ctctcgattc aaccgtctat cctcttcggt taagaggtga tgtttagaat gcttagcagg
     1561 aataaaggta ttttcgtgaa atttcaagat gcctaaatac cctaaatcta cgaaagcaag
     1621 ggtattttgg ggcaaactat agccaatact ctccttaaaa agggtaaaat catgcgtatg
     1681 accatctgaa aaagccaatt gacaagcacg atggctagtc aaatcaggca tgatttgagt
     1741 ctgctagtct ttttcttaat aactggccta ttattgttat tatatccaac agtaagtgat
     1801 tattggaact cacattatca atcagaagca gtagctactt attctgagaa attattggat
     1861 atttcaaaag aagaaaaaca aaaaatttta gaaaatgtca gtgcatataa tagcacgctc
     1921 gaaaagggga caatacctaa tttaaatata gacagtgtag ctttaaaaaa gtatgactca
     1981 attttaaata tcacagatac tggtattatg ggctatgtag aaattcctaa attgcagaca
     2041 atgttaccta tttatcacgg aacggatgat gccgtattgc aggtagcagt aggtcacctt
     2101 gctgggactt cccttcccat tggcggtcag ggaagtcacg ctgctatttc aggacatcgg
     2161 ggattgcctt ctgcaaaact gttttctgat ttagatcagc tggttatagg ggacacattt
     2221 atgatacaag ttttagataa cactctgaca tacgaaattg ataaaattat tacggttact
     2281 ccagatgatg tatctccttt gatgatagat ccgaatgagg atttcgtaac ccttgtgact
     2341 tgcacacctt atggtgttaa tagccaccga ttattagtta gaggtcatcg aattgaaaat
     2401 caactacttg ctaggcaagt agtctcagaa ggatcaattg taaaacctat actggtttct
     2461 ccttttattg ctttatgttt attaggacta ctttggagtt tattttttgt aaaaaaagaa
     2521 gtaaatttgt ttatgtgact tagatagtat cgcaaagaat gtcaaactat tcgactttgt
     2581 tctttagctt ttaagcttag gaaagggaga tttttccaac tcccaatcat ccaaaaggtg
     2641 aaatagaaga taaaccaaat gacgatatgt caatgtcctc tatacgctag aaaaaggtgg
     2701 caagcgagct gtcggattca aattgtcaga cggcattgac attcctaagg aatttgaagg
     2761 taaatttaag tttgcccgcc aacgctccaa actagctggc gaaattcgtg gcaccttctt
     2821 tgttgtcaag ggcgagtatt tataggatgg caaatctgag gagactcaga ttttttaaat
     2881 aatgacttga cctggagtgg acttcaaggt gtataataag cgaaaacaaa gaaaggagcc
     2941 tggaatgaat atcaaagaag ttagtgatgt aacaggtcta tcagcagaca ccattcgcta
     3001 ctatgagcgg ataggactta ttcccaaaat tgcaagaaaa tcttctggag taaggaactt
     3061 ttcggaaagt gatgtagcca ttttggaatt tatccgctgt tttaggagtg ctggtatgtc
     3121 tattgagcgc ttgattgagt atatgggctt ggtgcaggca ggagatagta cagtggaagc
     3181 tcggattgat ttactaaaag aggaac
//
LOCUS       DBNFEG010000185         3168 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.185, whole genome shotgun sequence.
ACCESSION   DBNFEG010000185 DBNFEG010000000
VERSION     DBNFEG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.185"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            39..980
                     /gene="metA"
                     /locus_tag="AC1PNG_001920"
     CDS             39..980
                     /gene="metA"
                     /locus_tag="AC1PNG_001920"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase MetA"
                     /protein_id="HIP6702387.1"
                     /translation="MPIKIEKSLPAVEILRKENIFVMDSDRASHQDIRPIKILILNLM
                     PQKIVTETQLLRLLANTPLQLEVEFLYMASHESKNTHSDHLKQFYKTFEQVRDSYFDG
                     LIVTGAPVENLPFEEVDYWQELTQVFDWSKTHVYSTLHICWGAQAGLHARYGVPKHSM
                     SRKLSGVYCQEVTNPTSPLMRGFDDEFRSPHSRYTEVREADIAHLSGLTILSQGPEVG
                     LSILASKDLREIYSFGHLEYDRDTLAKEYQRDCIAGKNPHLPENYFKEDDPTSKPSLS
                     WNLPAAQFFTNWVNYAVYQETPYDWHIFEQSAQSASL"
     gene            990..1664
                     /locus_tag="AC1PNG_001921"
     CDS             990..1664
                     /locus_tag="AC1PNG_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HIP6702388.1"
                     /translation="MNYSQQFRQGHLVLPAAILFHYQELFPSADDFLIWQFFLYQNSS
                     AIESLAPSEIAQATGKTVAQVNQAIENLQDAGLLEFKTISIAGEIEMIFDALPAFEKL
                     DVLLTPKQAVEIVQPENDLKTLVGDFERELGRFLSPFEIEDLQKTIEDDKTSIDLVRA
                     ALKEAVFNNKTNWKYIQAILRNWRREGITTVAQVEAKNAEREIQTPKNVTVSSDFLDA
                     MDLWKD"
     gene            1666..2349
                     /locus_tag="AC1PNG_001922"
     CDS             1666..2349
                     /locus_tag="AC1PNG_001922"
                     /EC_number="2.1.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0160105 - tRNA
                     (adenine(22)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase"
                     /protein_id="HIP6702389.1"
                     /translation="METKLSKRLETVASFVPQGARLVDVGSDHAYLPLFLVEQGRIDF
                     AIAGEVVQGPYQSALQNVEQAGQTEKILVRLANGLGAFEKSDQMTAVTIAGMGGRLIA
                     EILEAGKEKLATIERLILQPNNREDDVRRWLEKNGFRLVAEKILEENGKLYEVLVAES
                     GQMRLSEVEHRFGPYLLEEASPVFVKKWRRELEKLSFALEQVPSERQGDRFAISQKIQ
                     QIQEVVHVS"
     gene            2339..3133
                     /locus_tag="AC1PNG_001923"
     CDS             2339..3133
                     /locus_tag="AC1PNG_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIP6702390.1"
                     /translation="MLASQVIERYQAFCPPSLAMEGDVKGLQIGTLNKEIQRVMVALD
                     IRETTVAEAIDKKVDLIIVKHAPIFRPINDLVADNPQTKMYLDLIKHDIAVYVSHTDI
                     DVVEGGLNDWFCELLEIQDTTYLIETAEGQGLGRVGTIAEQTLEELALKVKSVFGLDA
                     VRLVTYGHQAKQYVSRVAICGGSGGSYYHEALAKGADVYITGDIYYHTGQDMLTQGLL
                     AIDPGHHIEALFIKKIAELLEIWKAKEGWDVEILPSTVSTNPFRHL"
BASE COUNT          945 a          576 c          743 g          904 t
ORIGIN      
        1 tgtcttataa taaatataag aaaattggga ggttgcttat gccaattaaa attgaaaaat
       61 cattaccagc agttgaaatt ttgagaaaag agaatatttt tgtgatggat agtgaccgag
      121 ccagtcatca ggacattcgt cctatcaaaa tccttattct caatctcatg ccacagaaga
      181 tagtgacgga gactcagttg ctccgtcttt tggctaacac tcctttacag ctagaggttg
      241 aatttctcta catggctagt cacgagtcca aaaatacaca ctcggaccat ttgaagcagt
      301 tttataagac ttttgagcag gtcagggata gctattttga tgggctcatc gtgacaggag
      361 caccagttga aaatttacct tttgaggaag ttgattactg gcaagaactt actcaagttt
      421 ttgattggtc aaaaacacat gtttattcaa cattgcacat ttgttggggt gcacaggcag
      481 gtttgcatgc acgctatggt gtacccaaac acagtatgtc tcgaaaacta tcaggggtat
      541 attgtcaaga agtgactaat ccaacgagcc ctcttatgcg tggatttgac gatgaatttc
      601 gttcaccaca ttcccgttat acggaagtaa gagaagcaga tattgcccat ctgagtgggt
      661 tgacgatttt atcacaaggt ccagaagttg gtctatccat tctagccagc aaggatttaa
      721 gagaaattta tagttttgga catttagagt atgataggga tacactagct aaggaatatc
      781 aacgtgattg tatagctgga aaaaatcccc atttaccgga aaattatttc aaagaagatg
      841 accctacaag caaaccatct ctctcttgga atttaccagc agcacaattc tttaccaact
      901 gggtgaatta tgctgtctat caagaaactc catacgactg gcatatcttt gaacaatcag
      961 cccagtcagc tagtctttaa agaggaagta tgaactattc acagcaattt cgtcagggac
     1021 atttggtcct gccagcagca atattattcc actatcaaga gctttttccg tcggcagatg
     1081 attttttgat ttggcagttt ttcttatatc aaaattcttc agctatcgaa tcattagcgc
     1141 ctagtgagat tgctcaagct actggtaaaa cagttgctca ggtcaatcaa gctattgaaa
     1201 atctccaaga tgctggactc ttagaattta agacaatcag tattgctggt gaaattgaaa
     1261 tgatattcga tgcgcttcct gcttttgaaa aattggatgt tctgttaact ccaaaacagg
     1321 cagttgagat tgtgcaacca gagaatgatt tgaagacctt ggtgggtgat tttgaacgtg
     1381 aattgggccg ctttttatcg ccgtttgaaa ttgaagattt gcagaagact attgaggatg
     1441 acaaaacttc gattgatttg gttcgtgcag ccttgaaaga agctgttttt aacaacaaaa
     1501 caaattggaa atacatccaa gctattctac gaaactggcg ccgtgaggga attactacag
     1561 ttgcgcaagt agaagcgaaa aatgcagaaa gagaaatcca gactcctaag aatgtgactg
     1621 tttcaagtga ctttcttgat gcaatggatc tctggaaaga ttgatatgga aacaaaatta
     1681 tctaaaaggc tggaaacagt cgcaagtttt gtcccacaag gggcacgtct agttgatgta
     1741 gggagtgacc atgcttatct ccctcttttt ttggtagagc aggggcgtat tgattttgcc
     1801 attgcggggg aagtcgttca aggtccctat cagtcagcgc tccagaatgt agagcaggcg
     1861 ggacaaacag agaaaattct agtccgtttg gcaaatggct taggcgcgtt tgagaagtct
     1921 gaccagatga ctgccgtgac catagcaggt atggggggac gcttgattgc ggaaatctta
     1981 gaagcaggaa aagaaaagct tgcgaccatt gaacgtttga tactccagcc aaataatcgt
     2041 gaagatgatg tgcgtcgatg gttggaaaaa aatggtttta gattggttgc tgaaaagatt
     2101 ctggaagaaa atggcaagtt atatgaagtc ctcgttgcag aatcaggaca gatgagattg
     2161 tcagaagttg aacatcgttt tggtccctat ctactagagg aggcctcacc agttttcgtc
     2221 aaaaagtgga gaagagaact tgaaaaatta agttttgctt tggaacaagt tccatctgaa
     2281 cgccaaggtg accgctttgc catttcccaa aagatccaac aaattcagga ggtagtccat
     2341 gttagctagt caggttattg agcgctatca agccttttgt cccccttctt tggctatgga
     2401 aggcgatgtc aaagggctgc aaatcggtac ccttaataaa gagattcaac gtgtcatggt
     2461 ggctctggat attcgtgaga caacagtggc agaggcgatt gataaaaagg tagatttgat
     2521 tatagtcaag cacgctccga tttttcgtcc aataaatgat ttggtggctg acaatccgca
     2581 aactaaaatg tatcttgatt tgattaagca tgatattgca gtctatgtta gtcatacaga
     2641 tattgatgtg gttgagggtg gtctcaacga ctggttctgt gaactattag aaattcaaga
     2701 tactacctac ttgattgaaa ctgccgaggg gcaggggctt ggccgtgtgg ggacaatagc
     2761 agagcagact ttggaagaat tggctttgaa agtcaagtct gtctttggtc tagatgctgt
     2821 ccgtctagtg acctatgggc accaagccaa gcagtatgtc agccgtgtag ccatctgtgg
     2881 aggcagtggt gggtcttact accatgaggc tcttgctaag ggggcagatg tttacatcac
     2941 gggcgatatt tactaccata ctgggcagga catgctgact cagggcttgc tggctattga
     3001 tccaggccat catatcgaag cattatttat caagaaaatt gctgaattgt tggaaatatg
     3061 gaaggcaaaa gaaggttggg atgtcgaaat tctaccttca actgtttcaa ccaatccgtt
     3121 tagacatttg tgataaatta atcagcactt gattgtggaa agaccaag
//
LOCUS       DBNFEG010000186         3162 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.186, whole genome shotgun sequence.
ACCESSION   DBNFEG010000186 DBNFEG010000000
VERSION     DBNFEG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.186"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            66..259
                     /gene="ssrS"
                     /locus_tag="AC1PNG_001924"
     ncRNA           66..259
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC1PNG_001924"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            268..340
                     /locus_tag="AC1PNG_001925"
     tRNA            268..340
                     /locus_tag="AC1PNG_001925"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:301..303,aa:Lys,seq:ctt)
     gene            complement(445..1002)
                     /gene="nrdG"
                     /locus_tag="AC1PNG_001926"
     CDS             complement(445..1002)
                     /gene="nrdG"
                     /locus_tag="AC1PNG_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIP6702391.1"
                     /translation="MNNPKPQEWKSEELSQGRIMDYKAFNFVDGEGVRCSLYVSGCLF
                     HCEGCYNVATWSFKAGIPYTKELEDRILADLGQPYVQGLTLLGGEPFLNTGTVLPLVK
                     RIRKELPEKDIWSWTGYTWEEMMLETPDKLELLSYLDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLQEKRVVLWDKLKK"
     gene            complement(1189..1683)
                     /locus_tag="AC1PNG_001927"
     CDS             complement(1189..1683)
                     /locus_tag="AC1PNG_001927"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6702392.1"
                     /translation="MELRRPTIADKETILEMLAEFEAAGSAMDGGFISKDVDFEEWIF
                     RNKDYEAGLNLPDGFVPSIQYVSFDQTGRALGFLSLRLRLNDFLLNKGGHIGYSIRPT
                     ERGKGYAKDQLRLGLQEAATKNISKVLVTCSTDNQASRRTIVACGGVLEDVRDGVERY
                     WIED"
     gene            complement(1685..3031)
                     /locus_tag="AC1PNG_001928"
     CDS             complement(1685..3031)
                     /locus_tag="AC1PNG_001928"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIP6702393.1"
                     /translation="MDYTDISILHTNDMHSYMENFPKKAQLIANIRARNEKEGVPTFV
                     FDSGDLFSGNIFFNMYRGVKEIELMNQIGCQAMTLGNHEFDHGDELISRLDDYAQFPI
                     VSSNLRYQDVEVADELVPLEDVLEFDMNGKNLYVLGLTTLETQEVASPSENVIFEDHK
                     QALRRLVDQIVSQNPSAHLVLLSHLGYDEDVHLAKDFPELNVILGGHTHTILKQPSQI
                     GGVSICQAGQYGRFVGHLSLRCFADGRHEVQAYDLIEVEKLTQEDRAVKAIIDQMKSE
                     RDAAFSKPIAILPQALDGERDSIRQGLSTLAPVICQALFEQASQLGLQADGAVINGFG
                     IRASLSAGPIFYSDLVKVLPFSKRVLLVRIKGRDLLSSLQTGLHPQMWGIVQSGEDLL
                     VNGRAVESDKDYQIVTNSFVWSGKDNYDDFHKAEIVQDLGLDIDIISEFFKRQYGG"
BASE COUNT          841 a          783 c          633 g          905 t
ORIGIN      
        1 cttacatttt tgccattttt ctcttgattt ttcaaaaaaa agtggtatta ttaaatcata
       61 aataagaact gctgtggtat acgaattcat acctatgtgt tgaccgacta tttttgtatt
      121 actagggaga caaagatcct ctaacagtat gcaagctggc acacccagaa gcaactaagt
      181 tagaaacgcg tcacccacct gcttttagcg tgcgggatca atacaatcca tggataaccg
      241 gtaccaatac agcattttta ttttttacct tccttagctc agctggcaga gcagcggact
      301 cttaatccgt gggtcgtagg ttcgatccct acagggagga ttggttgata tgaggaaagt
      361 cttgatttat caaggctttt tctcgttttt tgagtgaatt tggtcaaaat ttggtcaaaa
      421 agaacccttt cacattgtga agggctattt ttttaactta tcccacagga caacacgttt
      481 ttcctgtaat gatttctgta catcgatgat gcgttggttg gaagagcctc gaaattgcag
      541 cataagattg cgcttggtac ggtcataacg gccgtcaact agaatatcta aataggatag
      601 taactctaac ttatccggag tttctaacat catttcttcc catgtgtagc ctgtccaaga
      661 ccaaatgtcc ttttctggca gttctttccg aattcgcttt acgagtggga ggaccgtgcc
      721 tgtgttcaga aaaggctcgc cacccaagag agttaagccc tgcacatagg gctggcccaa
      781 gtctgctaaa atgcggtctt caagctcctt ggtataggga atccctgctt taaaagacca
      841 ggttgcaaca ttgtagcagc cctcacagtg aaacagacag ccactaacat atagagagca
      901 acggactcct tctccatcca caaagttaaa ggctttgtag tccattattc gcccttggct
      961 aagttcctcg ctcttccatt cttgtggttt aggattattc atttcaaata cctgagcata
     1021 agcaaatgaa atctgatcgt ttggatgaat tccgttttac cagtacacag actgttttta
     1081 tccataaatg gtacttccaa tgctttagct cggattgatc ccagcaagct tagtgcggaa
     1141 ccaatctcaa ttcctttcct gttttgattc agcagtttgt cactcccatt aatcctcaat
     1201 ccagtatctt tctactccgt ctcgaacatc ttccaaaacg ccaccacaag ccacaatcgt
     1261 gcgacggctg gcttgattgt ctgtcgagca agtcacaagg accttggaaa tatttttggt
     1321 tgcggcttcc tgtaaaccaa gtcgcagctg gtccttggca tagccctttc ctcgctcggt
     1381 cggacgaatg gaatatccga tatgaccgcc tttattgagc aggaaatcat tgagccgtag
     1441 gcgtagactg agaaaaccaa gggctcgacc agtctggtca aatgacacat attgaatgga
     1501 aggaacaaaa ccgtctggca gattgagacc tgcctcatag tctttgtttc taaaaatcca
     1561 ttcttcaaag tccacatctt tggagataaa gcctccgtcc atagctgagc ctgccgcttc
     1621 aaactctgcc agcatttcca aaatcgtttc cttatccgct atagtcggtc ttcttagttc
     1681 cattctagcc tccatattgt ctcttgaaaa attcactgat gatgtctata tccagtccca
     1741 agtcctgcac aatttctgcc ttgtggaaat catcatagtt gtccttaccg ctccaaacaa
     1801 aagaattggt cacaatctga tagtccttat ctgactcgac tgctctgcca ttgaccaaaa
     1861 gatcctcacc gctttggaca atgccccaca tctgagggtg gaggcctgtc tggagactgg
     1921 agagtaagtc tctgcccttg atacgaacta agagtactcg cttggaaaag ggcaagacct
     1981 tgaccaagtc cgaataaaag attggtcccg ccgacaagga agctcgaata ccgaagccgt
     2041 tgatgaccgc tccatccgcc tgcaaaccta gttgactcgc ctgttcaaat aaagcctgac
     2101 aaataacagg ggccagagtt gataaccctt gacgaatgct gtctcgctct ccgtctagtg
     2161 cctgaggtaa aatcgctatc ggtttggaaa aagctgcgtc acgttccgat ttcatctggt
     2221 cgataatagc cttgaccgcc ctgtcttctt gggtcagttt ctccacctca atcaagtcat
     2281 aagcctgaac ctcgtgccgt ccatctgcaa aacaccgcag ggatagatga ccgacaaagc
     2341 gaccatactg accagcttgg cagatactaa ctccgccaat ctgactaggt tgttttagaa
     2401 tggtatgggt atgtccgccc aaaatcacgt taagctcagg gaaatccttg gccaaatgca
     2461 catcctcatc gtatcccaga tgactgagca agaccagatg agccgatgga ttctgactca
     2521 caatctgatc caccaatcgc cgcagagctt gtttatggtc ttcaaaaatc acattctccg
     2581 acggtgacgc aacctcctgg gtttccaagg tagtcaaacc gaggacatag aggtttttcc
     2641 cattcatatc aaattcaagg acatcctcaa gcggcaccaa ctcatctgcc acctccacat
     2701 cctgatagcg gagattggag gacacaattg gaaactgggc atagtcatcc aagcgactaa
     2761 tcaactcatc tccgtggtca aactcatgat tgcccaaggt catagcctga caaccaattt
     2821 gattcattag ctcgatttcc ttgactcctc ggtacatatt gaaaaagata ttacccgaaa
     2881 agagatcgcc gctgtcaaag acaaaggtcg gaactccctc tttttcattc ctagcccgaa
     2941 tgttcgcaat cagttgcgcc ttctttggaa aattctccat gtaggaatgc atatcattgg
     3001 tatggagaat gctaatatct gtatagtcca tactattttt tcttattttt tgcttgtttg
     3061 agcaattcct gcacacgcgc ttttttatcc tgcttcacat tagaattctt ctcgtgatag
     3121 cgtgtcacca gggccttccc cttatcgtct aattgatatt tt
//
LOCUS       DBNFEG010000187         3160 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.187, whole genome shotgun sequence.
ACCESSION   DBNFEG010000187 DBNFEG010000000
VERSION     DBNFEG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.187"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..840
                     /locus_tag="AC1PNG_001929"
     CDS             <1..840
                     /locus_tag="AC1PNG_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIP6702394.1"
                     /translation="MNILNIYNELDSHNFDLNVDHYGAEQCDKGYSFGPTIRDNFVLH
                     FITKGKGKILVDGKTSYLAAGDLFILPKDVSIFYQADDIDPWTYIWVGFSGSRAKDLL
                     NQSQLLENYYLHSNLESPILGYMHQINHVQMHPIPSITELVLIGYLNQLLAALIEEFP
                     SETLIKPETQTKHYVQQAIKMIHNHYAHPIKVSEIADFLALSRSYLYKIFKQETGYSI
                     KDYILQVKMNRSCQLLEDASRSITEIAYSVGYQDPLTFSAAFKNYFHMSPTDFRNTQK
                     NKD"
     gene            complement(872..2032)
                     /locus_tag="AC1PNG_001930"
     CDS             complement(872..2032)
                     /locus_tag="AC1PNG_001930"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIP6702395.1"
                     /translation="MTQYNFIDSPNRLSQHAEKWRKVETDKELLPLWIADMDFEPLPE
                     IRQVIRDYADHHVFGYPYASDSLYQSIIDWEDRQHGYKINRESILLIEGVVPALSVAI
                     QSLTEEGDAVLINTPVYPPFARTVKLNNRQLVTNSLVDVDGVFRIDFDQLEKDIVENQ
                     VKLYIFCNPHNPGGRVWTREEVLAIGRLCQKHGVILVSDEIHQDLTLYGHKHYSFNTV
                     DESFKEFSIILSSATKTFNIAGTKNSFAIIENPSIRKTFAKRQLINNQHEIPTIGLLT
                     TEAAFTYGDEWLKELKVILERNIDFVEEYLITHTRIKVMKPQGTYLIWLDFSAYELEH
                     AELFDILHCKAKLILNDGLTFGKEGKYHARLNVAVPFNMIEEACQRLGKVFG"
     gene            complement(2036..3127)
                     /locus_tag="AC1PNG_001931"
     CDS             complement(2036..3127)
                     /locus_tag="AC1PNG_001931"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HIP6702396.1"
                     /translation="MTDYKIDTILAHTGINSDERTGALISPIHLSTTYQHPEFGQSTG
                     YDYTRTKNPTRASLETTLAAIEKADYALATSSGMAALVLLFNGFPVGSQVVAARDLYG
                     GSFRWFNEQESIGRFQFTYANTEEELIAAITEETDYVYLETPTNPLMVEFDIAKVSAI
                     AHAKGAKVIVDNTFYSPIYQNPLVLGADVVLHSATKYLSGHNDVLAGALMTNDQALYD
                     KLFYDQNTSGPTLSPLDSYLLMRGLKTLSLRMERATQNAQKIVAYLEKSPAVKQVYYT
                     GKGGMISVKVVDESRIPHILNTLKVFTFAESLGGVESLITYPATQTHADIPAETRHSY
                     GLTDDLLRLSIGIEDAEDLIVDLKTALEG"
BASE COUNT          934 a          714 c          596 g          916 t
ORIGIN      
        1 atgaatattc tgaatatcta taacgagctt gatagtcaca actttgacct aaatgtagac
       61 cattacggtg ccgaacaatg tgacaagggc tattccttcg gtcctactat ccgtgataat
      121 tttgtcctgc attttattac aaaaggaaaa gggaaaattc ttgttgacgg aaaaacaagc
      181 taccttgctg caggcgattt gtttattctt ccaaaggatg tttccatttt ctatcaagct
      241 gatgatatag acccgtggac ctatatatgg gttggtttta gtggttcacg agcaaaagac
      301 ctacttaatc aaagtcaact gctagaaaac tactacctcc actccaatct agagtcacca
      361 attctaggct acatgcatca gattaaccat gttcagatgc atcccatccc ctctatcaca
      421 gaattggtct taattggcta cctcaaccaa ctcttagcag cactcatcga ggaatttccc
      481 agtgaaacat taattaagcc tgaaacacaa accaagcact atgttcagca agccatcaag
      541 atgatccata accactatgc ccatccaatt aaagtttctg aaatagcaga ttttctagct
      601 ttaagccgaa gctatctcta taaaattttt aagcaggaaa ctggctattc tatcaaagat
      661 tatattttac aagtaaaaat gaatcgttct tgtcaactat tagaagatgc ttctcggagt
      721 atcactgaaa tcgcctattc tgtcggatac caagaccctc tcaccttcag tgcggccttc
      781 aaaaactatt tccatatgag cccaacagat tttagaaata cacaaaaaaa taaggattag
      841 gtgattttac tcacacctag tccttatttc tttatccaaa aaccttacca agtcgctgac
      901 aagcttcttc aatcatgttg aatggtactg ctacattcag acgtgcatga tacttacctt
      961 ccttaccaaa ggtcaaacca tcattcaaaa tcaacttcgc tttacaatga agaatatcaa
     1021 agagctccgc atgctctaac tcataagcag aaaaatcaag ccagatgagg taggttccct
     1081 ggggcttcat gaccttgatc cttgtatggg ttatcaaata ctcctcaaca aagtctatat
     1141 ttctctccaa aataaccttc aactccttca accactcatc tccataagtg aaagcagctt
     1201 ctgtggttaa caagccaatc gttggaatct cgtgctgatt atttatcaat tgacgcttag
     1261 cgaaagtctt gcggatgctt ggattttcaa tgattgcaaa gctatttttt gtaccagcga
     1321 tattgaaggt cttggtcgca gatgataaga tgattgaaaa ctctttaaaa ctttcatcca
     1381 ccgtgttaaa ggaataatgc ttgtgaccat acaaggtcaa atcttgatgt atttcatctg
     1441 agacaaggat aacaccatgt ttctgacaaa gacgtccaat agccaacact tcctctcttg
     1501 tccaaactct accacctggg ttgtggggat tacagaaaat atagagtttc acttgattct
     1561 ctacaatgtc cttttcaagc tggtcaaaat caatcctaaa aacaccatcc acatcaacta
     1621 gtgaattggt gactagctga cgattgttta attttacagt ccgcgcaaag ggtggataga
     1681 caggtgtatt aattaggact gcatcccctt cttctgtcaa agattgaata gccacagata
     1741 aggcaggaac aactccttcg atgagcagga tggactctct attaatcttg tagccatgct
     1801 gtctatcttc ccagtcaata atagactgat agaggctgtc actagcatag ggataaccaa
     1861 aaacgtgatg atctgcgtag tcacggataa cttggcgaat ttctggtaga ggctcaaagt
     1921 ccatatctgc tatccacaag ggtaagagtt ctttatccgt ctctactttt ctccattttt
     1981 ccgcatgctg ggatagacga ttcggactat ctataaaatt atactgtgtc atatcttatc
     2041 cctccaaggc tgtttttaaa tctacaatca agtcttccgc atcctctata ccaatagaca
     2101 gacgaagcaa atcatctgtc aaaccgtaag aatgacgggt ttcagctgga atgtctgcgt
     2161 gggtctgagt ggctggatag gtaatcagac tctctactcc acccagactt tcagcaaagg
     2221 taaagacttt cagggtgttg agaatatggg gaatccgact ttcatctacc accttaaccg
     2281 aaatcatccc accttttcct gtgtagtaaa cttgcttgac tgctggactc ttctccaaat
     2341 aggcaacaat tttctgagca ttttgtgtcg cccgctccat tcgaagcgag agggttttga
     2401 gtccacgcat gagtaagtaa gaatccagtg gagatagggt tggacccgat gtattttggt
     2461 cataaaagag cttatcatat agggcttgat cattggtcat caaagcgcca gctagtacat
     2521 cattatgccc agataggtac ttggttgctg aatgtaggac aacatccgct cctagaacaa
     2581 gtggattttg gtagataggg ctgtaaaaag tgttatcgac aatcacctta gcacctttgg
     2641 catgggcgat agccgaaacc ttagcgatgt caaactccac catgagtggg ttagtcggtg
     2701 tctccagata aacatagtct gtctcttcgg tgatggctgc aatcagctcc tcctcggtgt
     2761 ttgcataggt aaattgaaat ctaccgatac tctcttgctc attgaaccag cggaaggaac
     2821 caccgtacaa atcacgcgcc gctaccactt gactccctac aggaaaacca ttaaaaagta
     2881 agaccagagc agccattccc gagctcgttg ctagggcata gtcggccttt tcaatagctg
     2941 ccaaagtcgt ctctagactg gcacgtgtcg gattttttgt tctcgtgtag tcataacctg
     3001 tcgattgacc aaactcaggg tgttgatagg tcgtcgaaag gtggataggg gaaatcaagg
     3061 cacctgttct ctcatcgcta ttgatgcccg tatgcgctaa aatcgtatca atcttataat
     3121 ctgtcataac atctactcca atctaactta caatatagtc
//
LOCUS       DBNFEG010000188         3082 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.189, whole genome shotgun sequence.
ACCESSION   DBNFEG010000188 DBNFEG010000000
VERSION     DBNFEG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.189"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1022
                     /gene="adhP"
                     /locus_tag="AC1PNG_001932"
     CDS             <1..1022
                     /gene="adhP"
                     /locus_tag="AC1PNG_001932"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HIP6702397.1"
                     /translation="AVVVNPESTGVVVVDKELRSLEAGEALVQIEYCGVCHTDLHVAN
                     GDFGKVPGRVLGHEGIGIVTEIAPGVTSLKVGDRVSVAWFFQGCGMCEYCTTGRETLC
                     RTVKNAGYSVDGGMAEQCIVTADYAVKVPEGLDPAQASSITCAGVTCYKAIKEAHLEP
                     GQWIAIYGAGGLGNLAVQYAKKVFNAHVIAVDINNNKLELAKEVGADVTINGLEVEDV
                     PGYIKEITGGGVHSTVVTAVSKVAFNQAIDSVRAGGYVVAVGLPSEYMDLSIVKTVLD
                     GIKVVGSLVGTRKDLEEAFHFGAMGLVVPVVQKRPVKDAEAVFNEMVAGTIQGRMVLD
                     FCHSH"
     gene            complement(1103..2386)
                     /gene="hisS"
                     /locus_tag="AC1PNG_001933"
     CDS             complement(1103..2386)
                     /gene="hisS"
                     /locus_tag="AC1PNG_001933"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIP6702398.1"
                     /translation="MKLQKPKGTQDLLPQDSAKWQYVENFTRSIFKQYNYAEIRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGERHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PAKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSNNPATDVETIAMAYHFFEELGIKD
                     IRLHLNSLGNPESRAAYRQALIDYLTPLKDQLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDYLDEESTAHFEAVKTMLDSLGIAYVIDTNMVRGLDYYNHTIFEFMTEVG
                     GNDLTICAGGRYDGLVTYFGGPETPAFGFGMGIERLILVLEKQGIELPLDTQLDVYIA
                     VLGQEANGGALDLVQAIRKQGFRAERDYLDRKLKAQFKSADVFGAKAIITLGGSEIES
                     GQVVVKNNQTRSQVETSLEALKTDFASILEELEKQ"
     gene            2662..2847
                     /gene="rpmF"
                     /locus_tag="AC1PNG_001934"
     CDS             2662..2847
                     /gene="rpmF"
                     /locus_tag="AC1PNG_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIP6702399.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVAAPTVKFDETTGDYSRSHRVSL
                     KGYYKGRRKIAKAASAE"
     gene            2863..3012
                     /gene="rpmG"
                     /locus_tag="AC1PNG_001935"
     CDS             2863..3012
                     /gene="rpmG"
                     /locus_tag="AC1PNG_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIP6702400.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVI
                     FTEVK"
BASE COUNT          852 a          688 c          678 g          864 t
ORIGIN      
        1 aagcagtcgt tgtaaatcca gaatctactg gtgttgtcgt tgtcgataag gaacttcgtt
       61 cactcgaagc tggtgaggct ttggtccaaa ttgagtactg tggtgtttgt cacactgacc
      121 tccacgttgc caatggtgat tttggtaagg tccctggtcg cgttcttggt cacgaaggaa
      181 tcggtattgt tacagaaatc gcacctggtg ttaccagtct aaaagttggt gaccgtgtca
      241 gcgttgcttg gttcttccaa ggctgtggta tgtgtgaata ttgtacaacc ggtcgcgaaa
      301 ctctctgccg taccgtaaaa aatgccggct actctgttga cggtggtatg gctgagcaat
      361 gtattgtaac agcggactat gcagtaaaag ttccagaagg tcttgatcca gctcaagcaa
      421 gttctatcac ttgtgctggc gttacttgct acaaagcgat taaagaagct cacttggaac
      481 caggtcaatg gattgctatc tatggtgctg gtggacttgg aaaccttgct gtacaatacg
      541 ctaaaaaagt cttcaacgct catgttatcg ccgtagacat caataataat aaattggaac
      601 tcgccaaaga agttggtgca gatgtgacga tcaacggact tgaagtagaa gatgttcctg
      661 gatatattaa agaaattact ggtggcggtg tacactctac tgttgtaaca gctgtttcca
      721 aagtagcctt caaccaagct attgacagtg ttcgtgctgg tggctatgtg gtggctgtcg
      781 gacttccatc tgaatacatg gacctcagta tcgtgaagac tgtgctagac ggtatcaaag
      841 tggtcggctc actagttggt actcgtaaag atttagaaga ggccttccac ttcggtgcta
      901 tgggactagt tgttccagtc gttcaaaaac gccctgtcaa agatgccgaa gcagtcttca
      961 atgaaatggt tgccggtact atccaaggtc gtatggtctt agacttctgc cactcacact
     1021 aatgcgttct ccactatttt caagaagtga ttatccaatc gcttcttttt tgctttctaa
     1081 aataaaaaga agcagcttta gactactgct tctccaattc ttctaaaata cttgcaaaat
     1141 ctgttttaag tgcttccaag cttgtttcaa cttgacttct tgtctggtta tttttgacca
     1201 caacctgtcc agattcgatt tcgctaccac ccagggtaat gattgccttg gctccaaaga
     1261 catctgctga cttaaactgg gccttgagct tgcggtccag atagtcacgt tctgcacgga
     1321 aaccttgctt gcgaatggct tgaacaagat caagcgctcc gccattggcc tcctgaccca
     1381 aaaccgcgat gtaaacatct aactgggtgt cgagagggag ctcgatgcct tgcttttcaa
     1441 ggactaggat gaggcgctca atgcccatgc caaagccaaa agctggcgtt tctggtccac
     1501 caaagtaggt caccaagcca tcataacggc caccggcaca gatggtcagg tcattgccac
     1561 ctacctcggt cataaactcg aaaatagtat ggttatagta gtccaaacca cgcaccatgt
     1621 tggtgtcaat cacataagca atgcctaagc tgtccaacat agtcttaacc gcttcaaagt
     1681 gagcagtact ttcttcatct agatagtcca agatggacgg tgcattttcc acagcaacct
     1741 tgtcttcttt ttccttggaa tccaacacac gcaaaggatt ttcttctaga cggcgctggc
     1801 tatccttgga aagctggtcc ttgagcggcg tcagatagtc aatcaaagcc tggcgataag
     1861 cagcacggct ctcgggattg cccagactgt tgaggtggag gcgaatgtcc ttgatcccca
     1921 actcttcaaa gaagtgatag gccatagcaa tggtttctac gtctgtcgct gggttattag
     1981 aaccaaaaca ctccacacca atctggtgga actgacggag acgacctgcc tgcggacgct
     2041 catagcggaa cattggaccc atgtaataga acttagctgg cttttgcact tctggagcaa
     2101 agagcttatt ttctacataa gaacgaacaa cgggtgccgt cccttctgga cggagggtga
     2161 tatgacgctc tcccttgtca tagaaatcgt acatctcctt ggtcacaatg tctgtcgtat
     2221 ccccaaccga acggctgatc acctcgtaat gctcgaagat cggtgtgcga atctcagcgt
     2281 aattgtactg tttgaaaatg ctgcgggtaa agttttccac gtactgccat ttggcagaat
     2341 cctgcggtag taaatcctgt gttccctttg gtttttgtag tttcataata aagtgtatag
     2401 agccaactta tatcgctcga agttgtgctt cttcctttcc aaattaagtc ataagggctc
     2461 aaactctacc agtcgagcct tgcagtcctc cttattttac cataaatgct ctgctatttt
     2521 ccaaaaaagt gaaaaaaatc tgtcaagtaa ctttacttta caaaaaactt ttgttatact
     2581 gttaaagttg gcgaaagtca tacgcactat tttttgattt ataaggggct gagccctgac
     2641 atttaatgga ggaagaaaga aatggcagta cctgcacgtc gcacttcaaa agcgaagaaa
     2701 aacaaacgtc gtactcacta taaagtagca gctccaactg tgaaatttga tgaaactact
     2761 ggagattact cacgttcaca ccgtgtatct ttgaaaggat actacaaagg acgacgtaaa
     2821 attgccaagg ctgcttcagc tgaataatag aagggagata ccatgcgcgt aaatattaca
     2881 cttgaacaca aagaatctgg tgagcgtttg taccttactt caaaaaacaa acgtaacact
     2941 ccagaccgtc ttcaattgaa aaaatactca ccaaaattgc gtaagcacgt aatttttact
     3001 gaggtaaaat aagacaacat acagaaagcc tatgaaatca acgtttcaaa ggctttttct
     3061 tttgttttta acaagataac tg
//
LOCUS       DBNFEG010000189         3070 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.190, whole genome shotgun sequence.
ACCESSION   DBNFEG010000189 DBNFEG010000000
VERSION     DBNFEG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.190"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            184..>3070
                     /locus_tag="AC1PNG_001936"
     CDS             184..>3070
                     /locus_tag="AC1PNG_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 98 domain-containing
                     protein"
                     /protein_id="HIP6702401.1"
                     /translation="MKNNKRKFTLLTIATLASAFLFQNHVFASEVSLSDLNWTTATHG
                     DATSSKTVQKNKPFTAGNENRENKISLKMEDGSIREFEKGIGTVAANPSTISYDISNL
                     GATKFKSFVGIDRTAVPTDNRYSKISKFEVLVDGAVIYTSIDNYPDGIKYDTSAIWLE
                     LDIPSNAQIIEFKSYSGEHTWGDEVVLGNPTFEIPDSSEIQDDAVDVVDQTSPNELPV
                     RENSVYLSDLDWRSASHGDATASKTIQKNKPFTLGNNGSNQKISLKMSDGSIKEFEKG
                     LGTIAAGPSTIQYDISGAGVSRFTTFVGLDRSAGHADSRYANIEKFEIEVDGTVIYST
                     LNEYPDGFNYDTPAIKVDVEIPEGARSIRLNSYSGQQTWGDELVLAGAYFVASGQFRN
                     PNDFEPAPKRREISNTSPLLMMPLYANGSEYSKGNYSFWGEDTLVGKWENIDPELKPY
                     TVIQVHPDDLPHRAGVAQDFYEKILEQAQNYVNPTTGQNEPIPVILTVYTAGNQSHYT
                     AAHWITMEWIDRMYEKYSCLQGIFSTENYWVWAGNVESNAAEYLKLSARHGGYFIWSE
                     QNNGASIEKALGTQGRPIFKETVEKYWQNFIFMFKNTPAHEGNDAPTVSYMTGLWLAD
                     YAYQWGGLMDTWKWYETGKWKLFESSGIGKTQGNRQWLTQPEAMLGAEAINIYLNGGS
                     VYNFEHPAYTYGVRNEESPLYSTVIENFFKYVINNPAPSKEDVLAATKILINGNFSSK
                     RDGHFYVDVNTATHQSPLYTTGRYGVIPAVPSSISSEVLNSKLPDHIQVVSLNDGSMS
                     SAANRRNLLNGLYPEEYTGNIFGDEIDNRAFIYNYEYNRDNDQTGSFELSGKEFDVTL
                     KSHSFTIVENIATGLNIKQNNFRINKDSLWEGASNSTQARALPQLSKSDAINWVFNTY
                     IHNTPSSEQRETVYVLKNVSKQPTVEILNSSDSNFVTPSVEYNSESKT"
BASE COUNT         1072 a          484 c          600 g          914 t
ORIGIN      
        1 gaaataacgg aataattggt tctgcattcc tagtccaata atagagaaga aatatgatga
       61 aaatagatag ttcataatac tttcaatttt atagtttagt ttgttttcca atatcagaaa
      121 aaatggacta ggaaatgttg ggaagcaaaa gttctctttt atcaagaata agaggtaaaa
      181 caagtgaaaa ataacaagag aaaatttaca cttttaacga ttgcaacatt ggcttcagca
      241 ttcttgtttc aaaatcatgt gtttgcatct gaggtttcat tgtctgattt aaattggact
      301 accgcaactc acggtgatgc tacttcatcc aaaactgttc aaaagaataa accatttact
      361 gcagggaatg aaaaccgtga gaataaaatt tcattaaaaa tggaagatgg ctcaattaga
      421 gaatttgaga aaggaatcgg aactgttgcg gcaaatcctt ctacaattag ctatgacatt
      481 tctaacctag gagcaacgaa atttaaatcc tttgtaggaa ttgataggac agctgttcct
      541 acggataata gatattccaa aattagtaaa tttgaagtgc ttgtagatgg ggctgtaatt
      601 tatacatcaa ttgacaatta tccagatggg attaaatacg atacatcagc gatttggctt
      661 gaattagata ttccatctaa tgcacaaata attgaattta agagttactc aggagaacat
      721 acatggggtg atgaagttgt gttaggtaat ccaacttttg aaattcccga tagttcggaa
      781 attcaagatg atgctgtaga tgttgtagat cagacttctc caaatgaact acctgttaga
      841 gagaattctg tttatctttc agacttagat tggcgaagtg ctagtcacgg tgatgccaca
      901 gcttcaaaaa ctattcaaaa aaataaacca tttacgctag ggaataatgg aagtaatcag
      961 aaaatttctt taaaaatgtc tgatggatct attaaggagt ttgaaaaagg actaggtaca
     1021 atcgctgctg gaccatcgac aatacaatat gatatttctg gagcaggagt atcacggttt
     1081 accacatttg taggattaga taggagtgct ggtcatgcag atagtaggta tgctaatata
     1141 gaaaaatttg aaattgaagt tgatggtact gtaatttact cgactttgaa cgaatatcca
     1201 gatggtttta attatgatac tccagcaatt aaagttgatg ttgaaattcc agaaggtgct
     1261 cgttctattc gtttaaatag ttattctggt caacaaacct ggggcgatga gttagtttta
     1321 gcaggtgctt actttgtagc aagtggacaa ttccgtaatc ccaatgactt tgagcctgca
     1381 ccaaaacgta gagaaatttc taacacaagt ccattactta tgatgccatt atatgctaat
     1441 ggttcagaat acagtaaagg aaattattca ttctggggag aggatacttt agttggtaaa
     1501 tgggaaaata ttgatccaga attaaagcct tatacagtaa ttcaagttca cccagatgat
     1561 ttgccacata gagcaggagt agctcaggat ttttatgaga aaattctcga acaagctcaa
     1621 aactatgtga atccaaccac cggtcaaaat gaacctattc cagttatctt aactgtatac
     1681 acagcaggca accaatcaca ttatactgct gcacattgga ttactatgga atggattgat
     1741 agaatgtatg aaaagtattc ttgtcttcaa ggaatctttt caacagaaaa ttattgggtc
     1801 tgggctggaa atgttgaaag taatgcggct gagtatttaa aattgtcagc aagacacgga
     1861 ggatatttca tttggtctga gcaaaacaat ggtgcctcca ttgaaaaagc tttaggaact
     1921 cagggaagac cgatctttaa agagacagtc gaaaaatatt ggcaaaactt tattttcatg
     1981 ttcaagaata cccctgcaca tgaaggtaat gacgctccaa ctgtaagtta catgacaggc
     2041 ctatggcttg ctgactatgc ataccagtgg ggtggattga tggacacttg gaaatggtat
     2101 gaaacaggaa aatggaagtt gtttgaaagc tctggaattg gaaaaacaca agggaataga
     2161 caatggttaa ctcaaccaga agccatgtta ggagcagagg ctattaatat ctatttaaat
     2221 ggtggatcgg tatataattt tgagcatcct gcctatacct atggtgtaag aaatgaggaa
     2281 tcaccattat attcaactgt aattgaaaac ttcttcaaat atgtaataaa taatcctgca
     2341 ccttctaaag aagatgtact cgctgctaca aaaattttga taaatggaaa tttctcatct
     2401 aaacgagatg gtcatttcta tgttgatgtc aatacagcaa cacaccaaag tccactatat
     2461 acaactggtc gatatggtgt aatccctgct gtaccatcta gtatttcttc agaagtattg
     2521 aattcaaaat tacctgacca tattcaggtt gtcagtctaa atgacggcag tatgtcttcg
     2581 gcggctaaca gaagaaattt attaaacggc ctatatccag aagagtatac tggaaacatt
     2641 tttggtgatg agattgacaa tagagcattt atctataatt atgagtataa tcgcgacaat
     2701 gaccagactg gttcattcga acttagtggt aaagagtttg atgtaacttt gaaatctcac
     2761 tcgttcacga ttgtagagaa tattgcaaca ggattaaata tcaaacaaaa taacttccga
     2821 atcaataaag attcattatg ggaaggtgct tcaaattcaa cacaagctag agcattacct
     2881 caactatcaa aatctgatgc tataaattgg gtatttaata cttatatcca taatactcca
     2941 agctccgagc agcgcgagac agtatatgta ttgaagaatg taagtaaaca accaactgtt
     3001 gagatactga attctagtga ttctaatttt gtaactccaa gtgtagaata caattctgaa
     3061 tcaaaaactg
//
LOCUS       DBNFEG010000190         3069 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.191, whole genome shotgun sequence.
ACCESSION   DBNFEG010000190 DBNFEG010000000
VERSION     DBNFEG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.191"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..346
                     /locus_tag="AC1PNG_001937"
     CDS             <1..346
                     /locus_tag="AC1PNG_001937"
                     /inference="COORDINATES: protein motif:HMM:NF016432.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIP6702402.1"
                     /translation="LVNHQNYLRRREILFSVLPFSSFEKENFRLEDVLPDLTVDIEKI
                     VETKIMLELLSESLSRLNKSEMLLIESIYFQEKTLRQVAEELKTSPCRISRLRDKVLN
                     KLKNMLENSHKM"
     gene            682..930
                     /locus_tag="AC1PNG_001938"
     CDS             682..930
                     /locus_tag="AC1PNG_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HIP6702403.1"
                     /translation="MKTITHKELMKLGFAKTTARKIIRQAKNIAIQRFEVADKNSVNA
                     VKLSKSPFDNRRLDLAPLSIVEELLGFSLVEYEEKNHD"
     gene            923..2131
                     /locus_tag="AC1PNG_001939"
     CDS             923..2131
                     /locus_tag="AC1PNG_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIP6702404.1"
                     /translation="MTKNYKGVNRDKKGRIYIQTEFKANPITGKRQRYKSYLNRWGKP
                     FDTEKEAYNELCRVRAEHHDKFEYADYDISYKAFMNSVFLPYYKQTVQGLTYRTAMVH
                     FELFIEEFGDTKLRNITPRMCEQFRVKIIANYSPNYAKQLWCRFKQSLGYAERLEYIK
                     SFPCKSLDNPKGKRPETKFWTYEEFLKVINQFDLTDYEDHLRFVTVWLYYMTGVRVSE
                     GFSLIWSDFNAKKKTLHVQSTLEAIGKGQYLRKLQTKTEAGKRYISLDDETVRILSEW
                     RKIQVANSKDNYILSRFGEPMVKSTLSRMLKRYALKAGVPVITGKGLRHSHDSFMINV
                     LHLDVVALSHRSGRIDKATTLNTYSHYYQVDNTIGGQIQEVLEQSGVAKTKSDKTVGY
                     PTIHPTTIEG"
     gene            2318..3067
                     /locus_tag="AC1PNG_001940"
     CDS             2318..3067
                     /locus_tag="AC1PNG_001940"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIP6702405.1"
                     /translation="MKSYKNYIFDFYGTLVDIRTDENKLEVWNQLTQIYNAFGCSYRP
                     RQLKNAYHRFVEEAERFLAETVSYQYVEIDLETLFIRLLTDAPNENQSTNKPTDLETF
                     GQVVATIFRVLSREKLEAYENTLTSLQTLKDREARLFILSNAQRIFTQAEIEQTGCAD
                     LMEKIYISSDFKMKKPEPAFLQLVLEENNLDVKETVMVGNDLTSDIAIAHELGMDSIL
                     LNTFPYSQEEIDSYRRLGWNFEVVGDIAELL"
BASE COUNT         1119 a          458 c          598 g          894 t
ORIGIN      
        1 tctggtaaat catcaaaact atttacgaag acgtgaaata ttattttccg tacttccgtt
       61 ttcatcgttt gaaaaagaaa actttagatt agaagatgtt ttacctgatt tgactgtaga
      121 tattgagaaa attgtagaaa caaaaattat gttggagcta ctatcagaat cactttcgag
      181 actaaataaa agtgaaatgc ttttaattga gtctatatac tttcaggaaa aaacactacg
      241 tcaagttgct gaagagttaa aaactagccc atgcagaatc tctagattaa gagataaagt
      301 ccttaataag ttaaaaaaca tgcttgaaaa ttcgcataaa atgtgaaaca gaccgagaac
      361 tttttcactt tatatagtga aagagttttc tttatgtcct ttgaaaattg aatagaccaa
      421 ggtgggactt tatttatttg tcgagcagct gaataagata cgcgatgata tgagcgagaa
      481 actatctagt tcaaaaattg actggaaacc aatttgccat gacacaaata atgttaatga
      541 tacttctgaa cgttcaggct ctagtcgaaa aatggagcag gtgagattcc tatgttgatt
      601 tccaaatcaa cccaccaatg tcgtgaaact tagttcggag taacatgcat tcgaatcgtt
      661 tcggatagat agaggtattc tatgaagaca ataactcaca aagaattaat gaaattgggg
      721 tttgcaaaaa ctacagctag aaaaataata cgacaagcaa aaaatattgc aattcagaga
      781 tttgaagtag cagacaaaaa tagtgtaaat gcggtaaaat taagtaaatc cccattcgac
      841 aataggcgat tagacttagc accgttatct attgttgaag aattactagg tttctcgctc
      901 gtggaatacg aggagaagaa ccatgactaa aaattataaa ggtgtaaata gagataagaa
      961 aggtagaata tacatacaaa cagaatttaa agcaaatcct ataactggca aaagacaaag
     1021 atataaaagt tatttaaaca ggtggggtaa accgtttgat actgagaaag aagcgtataa
     1081 tgaattgtgc agagttcgtg cagagcatca tgataaattt gaatatgcag attatgatat
     1141 ttcttataag gcattcatga acagtgtatt cttaccatac tataaacaaa cagttcaagg
     1201 gctgacttat cgaactgcaa tggttcattt tgaattattt attgaggaat ttggtgatac
     1261 caaattgaga aacataacac caagaatgtg tgagcagttt cgagttaaga ttattgccaa
     1321 ttattcacct aactacgcaa aacagctgtg gtgtaggttt aaacaatcat taggttatgc
     1381 tgagcggtta gagtatataa aatcgtttcc atgtaaatca ttggataacc caaaagggaa
     1441 aagaccagaa acaaaattct ggacatacga agaattttta aaagtcataa accagtttga
     1501 cctaactgat tatgaagacc acttaagatt cgtaacagtt tggttatact atatgaccgg
     1561 agttcgagta agtgaaggat tctcattgat ttggtccgat ttcaatgcta agaaaaaaac
     1621 acttcatgtc caatcaacac tagaagcgat tggaaaagga caatatctaa gaaaattaca
     1681 gactaaaaca gaggcaggga agcgttatat ttcattggat gatgaaactg ttcggatatt
     1741 gtctgaatgg agaaagattc aagttgcgaa ttcaaaggat aattatattc tttcacgttt
     1801 tggtgaacca atggtaaaaa gtaccttatc cagaatgtta aaaagatatg cccttaaagc
     1861 aggtgttcca gtgattacag gaaaaggatt aagacactca catgattcgt tcatgataaa
     1921 tgtcttacat ttagatgttg tagcattatc acatcgatca ggaagaattg ataaagcaac
     1981 tacattaaac acctattcac attattatca agtagacaac acaattggtg gacaaattca
     2041 ggaagtgtta gaacaaagtg gcgtggcaaa aacaaaatca gataaaactg taggataccc
     2101 caccatacac cccaccacga tagaaggtta gttttgaaaa gtattgataa tacagggaaa
     2161 aattgaatga atgcaactgt tactcttaaa taatagagtt tccaatcaaa ataaaaacct
     2221 atcacttaat gtggtaggtt ttttcgttct attgttgcaa acgttttcgt tgtggggtat
     2281 aatataaaaa atgatgtcta aagattagga gaaagacatg aaaagttaca aaaactatat
     2341 cttcgatttt tatggtacat tggttgatat tcgtacagat gagaataaac tagaggtctg
     2401 gaatcagcta acacagattt acaatgcttt tggttgttcc tatcgaccgc gccagttgaa
     2461 aaatgcctat catcgttttg tagaagaggc agagagattt ctcgcggaaa cagtgagtta
     2521 tcagtatgtc gaaattgatt tagaaacact cttcattcgc ctattaacgg atgcaccaaa
     2581 tgaaaaccaa agtaccaata agccgactga tttagagact tttgggcaag tagtagccac
     2641 gattttccgc gtgctttcac gtgaaaagtt agaggcttat gaaaataccc taactagtct
     2701 acagacatta aaggatagag aagcgcgatt atttattctc tccaatgctc aacgaatttt
     2761 tactcaggca gaaattgaac agacaggttg tgctgatttg atggaaaaaa tttatatctc
     2821 ctctgacttt aagatgaaga agcctgaacc agcatttctt caactggttt tagaggaaaa
     2881 taatcttgat gttaaggaaa cggttatggt tggtaatgat ttgacatccg atatagctat
     2941 tgctcatgaa ttgggaatgg attccatttt gctgaatact ttcccgtata gtcaggaaga
     3001 aatcgactct tataggagac ttggttggaa ttttgaggtt gttggagata tagcggagct
     3061 attgtaaat
//
LOCUS       DBNFEG010000191         3049 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.192, whole genome shotgun sequence.
ACCESSION   DBNFEG010000191 DBNFEG010000000
VERSION     DBNFEG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.192"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            34..1056
                     /locus_tag="AC1PNG_001941"
     CDS             34..1056
                     /locus_tag="AC1PNG_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIP6702406.1"
                     /translation="MLEHSIDIQLKHPDDSFSLFGSNERHLRLMEQELGVVIHARTEK
                     VQVIGQEEHVEQARLVIQSLLVLVSRGLVINTPDVVTAISMAKNDEIEKFVALYEEEI
                     IKDNYGKPIRVKTLGQKLYVDSVKRHDIVFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IVLTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKEQTTRLMEREIIEIAP
                     LAYMRGRTLEDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDISQIDLPRKVKSG
                     LIDATEKLSKIPQIDFVHFSAKDVVRHPVVAQIINAYEQEALAADGRASFETIGEPSR
                     KYNGEE"
     gene            complement(1725..2873)
                     /locus_tag="AC1PNG_001942"
     CDS             complement(1725..2873)
                     /locus_tag="AC1PNG_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HIP6702407.1"
                     /translation="MTTSRFQLVGSLLRPANLGEFKRQIEAREDIKYPFYDDFDGYKE
                     TEASLIQKIVAEQKENGLDIVTDGEFGRSMWHLDFLWGFDGIERYIAEHGYPFKDHDG
                     QIFETRKDIGLRITAPLSAKNHHFIDIFKQVKELAGETVTKQTIFGPAHAFNELTIFN
                     GQVGPNQVYKTRDELKVGLIAAYKEFLDQYKEAGGQIVQFDDCLWELFDPANPVPFLP
                     QDDSEALAALADEFVAINNAVIDYGHEIGLTVWTHNCRGNYESRSAAGGTYEAIAEKF
                     LRDQKYDRFFLEWDDERAGDLKALESLRDKNVEVVLGLLSSKTSDLDDEERVYKLLEE
                     ASKIIPKERLYLSHQCGFASCDSGNELAIPQQWAKIKQGQEIAEKFWS"
BASE COUNT          918 a          609 c          638 g          884 t
ORIGIN      
        1 taatagagaa aagcacagga aaaggagcaa ctcttgctag aacattcaat tgatattcaa
       61 ctcaagcatc cagatgattc gtttagccta tttggttcga atgaacgcca tctgagattg
      121 atggaacagg aactaggtgt ggttattcat gcccgtacgg aaaaagtgca ggtgattgga
      181 caggaagaac atgtagaaca ggcacgtctt gttatccagt ctctccttgt cttggtgagt
      241 cgtggtttgg ttatcaatac accagatgtg gtcacagcta tttccatggc aaaaaatgat
      301 gagattgaga aattcgttgc cctctatgaa gaagagatta tcaaggacaa ctatggcaaa
      361 cccattcgcg taaagactct tggacaaaaa ctctacgtgg acagcgtaaa gcgtcatgac
      421 atcgtgtttg gaattggacc agctggtaca gggaagacct tcttggcagt tactctagct
      481 gtaacagccc tcaagcgggg acaggtcaag cggattgtcc tgacacgtcc tgcggtcgag
      541 gcaggcgaaa gtttaggctt tttaccaggg gacttaaaag aaaaagtaga cccatatttg
      601 cgtcctgtct atgatgccct ctaccagatt ttgggcaagg agcagaccac gcgcctaatg
      661 gagcgcgaga ttatcgagat tgcaccgttg gcctatatgc gggggcggac cttggaagat
      721 gcctttgtca tcttggatga ggcgcaaaat accaccatta tgcagatgaa gatgttcctg
      781 acccgtctgg gctttaattc caagatgatt gtcaatgggg atattagtca gattgacctg
      841 ccacgcaagg ttaagtcagg cctgattgat gcgacagaga aactaagcaa aattccgcag
      901 attgatttcg tacatttttc agccaaggat gtggttcgtc atccagttgt tgcccagatt
      961 atcaatgcct atgagcagga ggctctggct gcggatggtc gagcaagttt tgagacgatt
     1021 ggggagccta gtcggaaata caatggcgaa gagtagttgt ttcattggct gatatataaa
     1081 agaattgtga tgctagttac atttaatata caataaaaag cccaaatgga cgatactgta
     1141 agcgatgaaa caaacaggaa cttagtccaa ataagcgact tacagcatat cgtaaaatca
     1201 catcgtacag caaatatcaa ctatttgtca caagttatac caatcctatt gcccgacccc
     1261 cttaaagcat tgtcacaacg ttgctttgtc aacggtatca tataaatcta ttaaaatatt
     1321 gctagtactt caagttggat tagagattaa atcttaacga cattttcgaa ccagccctca
     1381 ttgtagcgga tagaggtcat aaaaactata actttatgga ttgttaccaa taaaaaggct
     1441 ggttctatct gattcggatt cgagatggga aaagcagcat gaaaccgagt tttactccct
     1501 ttcttttcgc atgttcggca ccgataagta tgaaaccctc gtggccaatt gtgataaacc
     1561 tgtagactat ataaaaagtc tccttttgtg ttgtccgaac ataaaatccc aagtattaca
     1621 tcggaaaaat acttgggatt agatatcata gattaactga tcagagttaa cttagagaca
     1681 ctgattgatg acagggcttt tatgcacgaa taacaagaag tggcttaaga ccaaaacttc
     1741 tctgcaattt cttgaccttg tttaattttt gcccactgtt gcgggatggc taattcatta
     1801 cctgaatcac atgaagcgaa tccacattga tgtgacaagt agagacgctc ttttggaata
     1861 atcttgcttg cttcctcgag taatttgtaa acacgttctt catcatccaa atcagaagtt
     1921 ttacttgaaa gtagaccaag aacgacttcg acattcttgt cacgcaagct ttccaaagct
     1981 tttaaatcac ctgcacgttc atcatcccac tcaaggaaga atcgatcgta tttttggtca
     2041 cgcaggaatt tttcggcaat agcttcgtaa gttccgccag ctgcagagcg gctttcatag
     2101 ttaccacggc agttgtgcgt ccagacggtc aagccaattt catgtccgta atcaataaca
     2161 gcattattga tagcaacaaa ctcgtcagcc aaagcggcca atgcttcaga atcgtcttgt
     2221 ggcaagaaag gtacagggtt agcagggtca aataattccc aaaggcagtc atcaaattga
     2281 acgatttgtc caccagcttc cttgtactgg tctaaaaatt ccttataggc agcgatgagc
     2341 ccgactttta attcatcacg agtcttgtag acctgatttg gtccaacttg tccattgaaa
     2401 attgtcaatt cattaaaggc atgggctgga ccgaaaatag tttgtttggt tacagtttct
     2461 cctgctaatt ctttcacttg tttgaaaata tcaatgaaat ggtgattttt tgcagaaagt
     2521 ggagcagtaa tacgcaatcc aatatcctta cgtgtttcaa aaatctgtcc atcatggtct
     2581 ttaaatggat aaccatgctc agcaatgtaa cgctcgatac cgtcgaagcc ccagagaaaa
     2641 tctaggtgcc acattgaacg accaaattct ccatctgtca caatatccaa accgttttct
     2701 ttttgttctg caacaatttt ttggatgagt gaagcttcag tttctttgta accatcgaag
     2761 tcatcataga atgggtattt gatgtcttca cgagcttcga tttgtcgttt aaattctcct
     2821 aagtttgctg gtcgaagaag tgaaccgact aattggaatc ttgaagttgt catatatgta
     2881 tctctctttc tttcgtttta gtgatgaacc tattctaact cataagctat catttgaaaa
     2941 atagttattt tcatggcaaa tccatagtta aaaactataa cttgaaaaaa tagaaggaaa
     3001 taactttcta tcatttgtca actatggtat aatcaaaagt gctgaaatc
//
LOCUS       DBNFEG010000192         3017 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.193, whole genome shotgun sequence.
ACCESSION   DBNFEG010000192 DBNFEG010000000
VERSION     DBNFEG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.193"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1790
                     /locus_tag="AC1PNG_001943"
     CDS             <1..1790
                     /locus_tag="AC1PNG_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III domain-containing protein"
                     /protein_id="HIP6702408.1"
                     /translation="LLNDIFIFEDNWDMEPCSVGYHLAPLDWTADCHGDEEWTFMLNR
                     QEYLWKLVLAYLVEKDSRYMEQVKRFIFSWIEQVTNWHPKRTSSRTLDTAIRCLSWLN
                     ILPFLIDWGGLSEAERSQLLVSIQKQLSYLHSHYRAKDTLSNWGIFQTGAMLLAHAYI
                     GDQVDLVELHHFAHQEIQEQIQTQILEDGTQFEQSFLYHVEVYKLLLGLAFSLPDYRK
                     QWTPILKKMADYVLQMTGPDGKSVALGDSDVHCTSDILQMTAIFFEDGTYLVDNSKLD
                     LGVLFLVGENGLRIFEHLTAQPNSSGGQHFSESGHSIVKEVDSYLCFKAGPVGSAHSH
                     SDQNSLCFYHKGQPIVIDSGRFSYRECEERYLLKSAWAHSTCILDDNAPEQIKGSWEY
                     DTYPQFISHSYKGVERCHWMQGIYQAQTSQGLSYWHKRQLLMVGGDLLLIVDSVDCSG
                     QHELISQLILDERVKIGEERLNDLRLYSQKDFEIQKTVRSPRYNQLVETNKLVKKESF
                     QDRLVTFTLLADEAIRVKQLPVYQTDYHLIESGLAFSCQGKGLNILLVVSDNDIYKGE
                     KLLQVAGYKLRGKCLVVDRDKRQVVRLKN"
     gene            1886..2887
                     /locus_tag="AC1PNG_001944"
     CDS             1886..2887
                     /locus_tag="AC1PNG_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIP6702409.1"
                     /translation="MSEKFTIKDIAELAQTSKTTVSFYLNGKYEKMSKETRERIERVI
                     RETDYKPSVVARSLNSKNTKLIGVLIGDITNTFSNQIVKGIEEEAHKSGYQVIIGNSN
                     YNQESEDKYIESMLMLGVDGFIIQPTSHFRKYSRIIEDKKKHMVFFDSQLYEHRTSWI
                     KTNNYDAVYDATQLCVENGYQKFILITADISRLSTRIERATGFMDALLDCKQSYQDLT
                     IADENTDIEEIKRFLQGVVNPQEKTLVFVPNCWALPLVFNAMKELKYDFPQVGLLGFD
                     NTEWTNFSSPTISTIVQPAFEEGCQATRMLINQIEGGNQVGRQQVLDCTVHWKDSTF"
BASE COUNT          947 a          522 c          680 g          868 t
ORIGIN      
        1 atttattaaa tgatattttt atctttgaag acaattggga tatggaacct tgttcagtcg
       61 ggtatcatct tgctcctctt gactggacag ccgactgcca tggggatgag gaatggacct
      121 ttatgctcaa tcggcaggaa tacttgtgga aattagtttt ggcctatctg gttgaaaaag
      181 attcgcgcta catggagcag gttaaacggt ttatctttag ttggatagaa caagtgacaa
      241 attggcatcc taaaagaacg agcagtcgca ccttagatac ggctatccgc tgtctaagct
      301 ggctcaatat tctcccattt ttaattgatt ggggcggact aagtgaagct gagcgtagcc
      361 aactgttagt atccattcaa aaacaattat cttatcttca cagtcattac cgtgccaaag
      421 atacgttgag caattgggga attttccaga ctggcgcaat gctcttggcc catgcttata
      481 tcggtgatca ggtggattta gtagagcttc accattttgc tcatcaggaa atccaagagc
      541 aaattcaaac tcagatattg gaagatggaa ctcagtttga gcagtctttt ctctaccatg
      601 tggaagttta taaactcttg ctaggactag ctttcagctt gccagattat cgaaaacagt
      661 ggacacctat tttgaaaaag atggcagact atgtcttaca gatgacggga ccagatggca
      721 aaagtgtggc tcttggcgat agcgatgttc attgtacttc tgacatctta cagatgactg
      781 cgattttctt tgaagatggc acctacctag tagataattc caagctagat ttaggcgttc
      841 tcttcttggt cggagaaaat ggcttgcgca tatttgaaca cttgaccgct caacccaact
      901 cgtcaggggg acagcatttt tctgaatcag gtcacagtat tgtcaaagaa gtggacagct
      961 atttatgttt caaggccggt cccgtgggaa gtgcccacag ccactctgac caaaatagct
     1021 tgtgctttta ccataaagga caaccaatcg taattgacag tggtcgtttc agctaccgtg
     1081 aatgcgagga gcgctacttg cttaaaagtg cttgggctca cagcacttgt atccttgatg
     1141 acaatgctcc agagcagata aaaggttcat gggaatatga cacgtatcct cagtttatta
     1201 gtcacagtta taagggggtt gaacgctgtc attggatgca gggcatttat caggctcaga
     1261 ccagccaagg cttgtcttac tggcataaac gacagctcct tatggttgga ggcgatttat
     1321 tgctgatagt ggatagcgtt gattgctcag gccagcatga gttgatcagt caattgattt
     1381 tagatgaaag agtcaagatt ggagaagagc ggttaaatga tcttcggcta tatagtcaaa
     1441 aggattttga aatccagaaa accgtgcgtt ctcctcgata caaccaacta gtagagacca
     1501 ataaactggt caaaaaagag agttttcaag atagattagt tacatttact ctattagcag
     1561 atgaagcaat tcgtgtcaag caattgccag tttaccaaac agactatcat ttgatagaaa
     1621 gtggtttggc ttttagctgt caaggaaagg gactgaatat tctgttagtg gtttctgata
     1681 atgatatata taagggagag aaattgcttc aagtggctgg ttataagttg agaggtaagt
     1741 gcttagtagt ggatagggat aagagacaag ttgtccgact caaaaattag aagggacaga
     1801 aaaaatacag tcatttctgt gcaaaataac tgataaagta cggatagtat gatagaataa
     1861 agtaactaaa ggaggtatcg gtaaggtgtc agagaagttt accatcaaag atattgcaga
     1921 actggctcag acttcaaaaa caacagtttc cttttactta aatggtaaat atgaaaagat
     1981 gtccaaagag acacgcgaaa gaattgagag agtcattcgc gaaacagact ataaacccag
     2041 cgttgttgcg cggagcctaa attccaaaaa tactaagttg attggagtat tgattggtga
     2101 tattacaaat acattttcca atcaaattgt caaaggtata gaagaagaag cgcataagag
     2161 tggctatcaa gttatcatcg gaaacagcaa ttacaatcaa gaaagcgaag ataagtatat
     2221 tgagagcatg ctgatgttgg gtgtagatgg ttttattatt cagccgacat cgcattttag
     2281 aaaatattcg cgcattattg aggataagaa aaagcatatg gttttctttg atagtcagct
     2341 ttacgaacac cgtaccagtt ggattaagac aaataattat gatgctgtct atgatgcaac
     2401 tcaactatgt gtagaaaatg gttatcaaaa atttattctt attacagctg acattagtcg
     2461 cttaagtaca cggattgaac gagctacagg gtttatggat gctctgttag attgtaaaca
     2521 aagttatcaa gatttaacaa ttgcagatga aaatacggac atagaggaaa ttaaacgttt
     2581 tcttcaaggg gtggtaaatc ctcaggaaaa gaccctagta ttcgttccga attgctgggc
     2641 gttaccctta gtttttaatg ctatgaaaga acttaaatat gactttcctc aggttggtct
     2701 tttggggttt gacaatactg aatggacaaa tttttcatcc cctactatct ctaccattgt
     2761 tcaacccgcc tttgaagaag ggtgtcaggc aacaaggatg ctgatcaatc agattgaggg
     2821 cggaaaccaa gtgggacgac aacaggtttt agattgcaca gttcattgga aagattctac
     2881 attctaagtt aagtcaaatt ttacttctta ggaagaggag cagaacgaag tagaatattc
     2941 tgattgagag ctcactgaaa ccctccttga atcgcctaat atatacaaaa aataggaatt
     3001 ttcccttaac tggtggg
//
LOCUS       DBNFEG010000193         2999 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.194, whole genome shotgun sequence.
ACCESSION   DBNFEG010000193 DBNFEG010000000
VERSION     DBNFEG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.194"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..329
                     /gene="tilS"
                     /locus_tag="AC1PNG_001945"
     CDS             <1..329
                     /gene="tilS"
                     /locus_tag="AC1PNG_001945"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIP6702410.1"
                     /translation="EGIFSYGSYIFSLNHPLEEADSILYFPTENPIVVRRRQAGDTIL
                     INGVNKKLRRWFIDNKISQKVRQNALIIEQNGEIYGITNLVSSDLSKSVKNDIIKATL
                     YIKMKE"
     gene            337..879
                     /gene="hpt"
                     /locus_tag="AC1PNG_001946"
     CDS             337..879
                     /gene="hpt"
                     /locus_tag="AC1PNG_001946"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIP6702411.1"
                     /translation="MLDKDIKKILVSEEEIVAKCKELGQILATDYADKNPILVGILKG
                     SIPFMAELMKHIDAHVETDYMVVSSYHGGTESCGTVKIIKDLDNSVAGRHIIFIEDII
                     DTGRTLKELKELFALRQAGSIKIATLLDKPEGRVVEIEPDYTCFTIPNEFVVGFGLDY
                     DENYRNLPYVGVLKEEVYTK"
     gene            901..2871
                     /gene="ftsH"
                     /locus_tag="AC1PNG_001947"
     CDS             901..2871
                     /gene="ftsH"
                     /locus_tag="AC1PNG_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIP6702412.1"
                     /translation="MNNQPNKGFIKNPFLVILVIATIVTAFQFFNAGQQVATQEISYS
                     QVVTELKNNNVSEITYQPNSSVIEITGKYKTEQEAKDELASSIKLFQVSSKVKYKNFK
                     SLILPSETNLSELQALANENGVKVTIKPESSNGLWLNIVFNLLPLIIAGVFFMMMMNQ
                     GGGARGAMNFGRNKAKALEQSNIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDA
                     KKAAPAIIFIDEIDAVGRQRGVGMGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATN
                     RSDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAADVDLKLVAQQTPGF
                     VGADLENVLNEAALVAARRNKTVIDASDIDEAEDRVIAGPSKKDRQVSAKEREIVAYH
                     EAGHTIVGLVLSNAREVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGG
                     RVAEEIIFNTQTTGASNDFEQATQMARAMVAEYGMSEKMGPMQYEGSHAMFGGQTTQK
                     HISEQTAYELDNEVRDLLNEARNKAADIIQSNRDTHKLIAEALLKYETLDSVQIKSLY
                     ETGKMPENIERDDEDVHPLSYEEVKEKINTKE"
BASE COUNT         1005 a          502 c          622 g          870 t
ORIGIN      
        1 cagagggtat tttttcttac ggttcatata ttttttctct caatcatcct ttggaagaag
       61 cggatagtat tttatatttt cctacagaaa atccaattgt agtgaggaga aggcaggctg
      121 gggataccat tttaataaac ggagtaaata aaaaactccg ccgttggttt attgataata
      181 aaatctcaca gaaagttcga caaaatgcgc ttattatcga gcagaatgga gaaatttatg
      241 gaattacgaa tcttgttagc agtgatttga gtaaatcagt aaaaaatgat ataatcaaag
      301 ctaccttata tataaaaatg aaagagtaga acaactatgt tggacaaaga tattaagaaa
      361 attcttgttt cagaagaaga aatcgttgca aaatgcaaag aactcggtca aattctagca
      421 acagactatg cagataagaa tcctattctt gtaggaattt taaaaggttc tatcccattt
      481 atggcagaat taatgaaaca tattgatgcc catgtagaaa cggattatat ggttgtttct
      541 agctaccatg gtggtactga gagttgtggt accgttaaga ttatcaagga tttggataat
      601 agtgttgcag gtcgacatat catctttata gaggatatta ttgacacagg ccgcacatta
      661 aaagaattga aagaactctt tgctttgcgt caagcaggtt ctattaaaat tgctactctt
      721 cttgataaac cagaaggtcg tgttgttgaa atcgagccag actatacttg ctttactatt
      781 ccaaatgaat ttgtagttgg atttggctta gattatgatg aaaattatcg taatcttcct
      841 tatgttggtg tactcaaaga ggaagtttac acaaaataga aaaaggtttc ctactatatt
      901 atgaacaatc aaccaaataa aggatttata aaaaatcctt ttctcgttat tctcgttatt
      961 gccactattg ttacagcctt tcaatttttt aacgctggtc agcaagtagc aactcaagaa
     1021 atcagttatt cacaagtagt aactgaattg aaaaataata atgtatctga aattacttat
     1081 caaccaaatt caagtgttat tgaaattact ggtaagtaca aaacagaaca ggaagctaaa
     1141 gatgagttag cttcatctat taagttattc caagtttcta gtaaagttaa gtataaaaac
     1201 tttaagtcac taattttacc gtcagaaacg aatttgtcag agctacaagc tcttgcaaat
     1261 gaaaatggag taaaggttac tattaaacct gaaagttcaa atggactttg gttaaatatc
     1321 gtctttaatt tgttaccgct tatcattgct ggtgtattct tcatgatgat gatgaaccag
     1381 ggtggtggag cacgtggtgc aatgaacttt ggacggaata aagctaaagc tttggaacaa
     1441 agtaacatca aggtacgttt ctcagatgta gcaggtgcag aggaagaaaa acaagaatta
     1501 gttgaagttg ttgaattttt gaaggatcca aaacgcttta ctaaacttgg agcacgtatt
     1561 ccagcaggtg tactactaga gggtcctcca ggaaccggta aaaccttgct tgccaaagca
     1621 gtagcgggtg aagcgggtgt accattcttc tccatttctg gttcagattt tgttgaaatg
     1681 tttgttggtg tcggtgccag ccgtgttcgt tcattatttg aagatgcaaa aaaagcagca
     1741 ccagccatta ttttcatcga tgaaatcgat gctgtcggtc gccaacgtgg tgtcggcatg
     1801 ggtggcggta acgatgaacg tgaacaaacc ctcaaccaac tcttaattga aatggatggt
     1861 tttgaaggga atgaagggat tattgtcatt gccgcaacca accgtagtga tgttttagac
     1921 cctgcccttc ttcgtccagg tcgttttgac cgtaaagtat tggttggtcg tccggatgtt
     1981 aaaggacgtg aagcaatcct taaagttcat gcgaagaata aaccactagc agcagatgta
     2041 gatttgaaat tagtagcaca acaaacacca ggatttgttg gtgcagattt agagaatgtt
     2101 cttaacgaag cagcccttgt agctgcccgt cgaaacaaga ctgtcattga tgcttcagat
     2161 attgacgaag cagaagatcg tgtcattgca ggtccttcta agaaagaccg tcaagtttca
     2221 gccaaagaac gcgaaatcgt tgcctaccat gaagcaggtc atacaattgt tggtctcgta
     2281 ctatcaaatg cacgtgaagt tcacaaagta acaattgttc cccgcggtcg cgcaggtgga
     2341 tacatgattg cccttcctaa agaagatcaa atgcttcttt ctaaagaaga tatgaaggag
     2401 caattggctg ggcttatggg tggtcgtgtt gcggaagaaa ttatttttaa tacacagacc
     2461 acaggtgcct caaatgactt tgaacaagct acacagatgg ctcgtgctat ggttgcagaa
     2521 tatggtatga gtgaaaaaat gggaccaatg cagtatgaag gcagtcatgc aatgtttggt
     2581 ggacaaacaa ctcaaaaaca tatttctgag caaactgcat atgagttaga taatgaagta
     2641 cgtgatttgt taaatgaagc tcgaaataaa gctgcagata ttattcaatc aaaccgtgat
     2701 acacacaaac tgattgcaga agcattgctt aaatacgaaa ccctagacag tgttcagatc
     2761 aaatctctct atgaaacagg caaaatgcca gagaatattg aacgtgatga tgaagatgtt
     2821 cacccccttt cttatgaaga ggtaaaagag aaaattaata cgaaagagta gtataaaaat
     2881 actcttggca agacattccc tcactctcaa ccactaaaat aaccacaaat ccttcgctaa
     2941 gtacataatc ttggcgaggg ctttttttac aaaaaagggc atttacaaga aaaacgaat
//
LOCUS       DBNFEG010000194         2936 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.195, whole genome shotgun sequence.
ACCESSION   DBNFEG010000194 DBNFEG010000000
VERSION     DBNFEG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.195"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            140..1288
                     /gene="cps2T"
                     /locus_tag="AC1PNG_001948"
                     /gene_synonym="wchF"
     CDS             140..1288
                     /gene="cps2T"
                     /locus_tag="AC1PNG_001948"
                     /gene_synonym="wchF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta 1-4 rhamnosyltransferase Cps2T"
                     /protein_id="HIP6702413.1"
                     /translation="MKHVFIIGSRGLPAKYGGFETFVQQLVSHQQSEKICYHVACLSD
                     TSHHQHSTYLGADCFTINPPKLGPARVIAYDMMAITYALKIIKEQQMERPIFYILGNT
                     IGGFIVPFAKQIHSVGGTLFVNPDGLEWKRAKWAKPVQRYLKFSEKMMVKYADLIIAD
                     NEGIEDYIQSEYSATNTRFIAYGTDLSPTKLTKESEQVRQYFTNWNIKEKEFYLIIGR
                     FVPENNYVTAIKEFMASNTRRDLVIVANHEGSAYFQKLKDETGFISDKRIKFVGTVYD
                     QELLKYLRQECRAYIHGHEVGGTNPSLLEALAQTNENLVLGVDFNRKVALEGARYWSK
                     ESGSLSQLINQIDENAEAVELGEIAKKHMKDEYTWEKIVQEYEELFLQ"
     gene            1285..2214
                     /locus_tag="AC1PNG_001949"
     CDS             1285..2214
                     /locus_tag="AC1PNG_001949"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIP6702414.1"
                     /translation="MKVNILMSTYNGQQFLAEQIRSIQEQTYTDWTLFIRDDGSSDRT
                     REIIKDFVEQDQRIHFIDVETDENLGVIKSFHRLIHYDTADFYFFSDQDDVWLPNKLE
                     VSLKKAQAYPTNLPLMVYMDLKVVNQNLEVMAESMVKSQSHHANTELVQELTENTVTG
                     GVAMINHHLAEMWQVTEDILMHDWYLALLASALGKLVFIDKPGELYRQHADNVLGART
                     LSKRFKKWIRPHILFAVYWDLIKNSQKQARHLLQMPLSQSNRELIEAFVTIMDKPMLE
                     RFRILRKYGLRKNKTFHTLVFTTLIVTKFAYKE"
     gene            2217..>2936
                     /locus_tag="AC1PNG_001950"
     CDS             2217..>2936
                     /locus_tag="AC1PNG_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6702415.1"
                     /translation="MNLLNKENQILLREMIKTDFKLRYQGSLIGHLWSILKPMLLFTI
                     MYLVFVRFLRFDDGTPHYAVGLLLGMVTWNFFTEATNMGMMSIVSRGDLLRKLNFSKE
                     IIVFSSVAGAAINYAINLMVVFVFAAINGVPFTWSILIIIPLFFELALLATGIAFILS
                     SLFVRFRDIGPIWEVFLQAGLYATPIIYSLTFILQRGQVTFAKLMMLNPVAQIIQDLR
                     HFIVYSGNTTVSELIENPVIVA"
BASE COUNT          943 a          474 c          592 g          927 t
ORIGIN      
        1 tcaaaatttg tttttatcta tactgtggag tcagaggcgt agaaaaagaa aagcctttat
       61 aaccaaacta ggatagaatt aggaaaaaaa actaaaaaat ggtataatga aaagaactgc
      121 aatttgatgg agaggattca tgaaacacgt ttttattatc ggtagtcgtg gactgcctgc
      181 aaaatatggt ggctttgaga cctttgtcca acaattggtc agtcaccagc aatctgagaa
      241 gatttgttat catgtggctt gtttatcgga tacaagtcat caccaacatt cgacctacct
      301 aggtgcagat tgcttcacca ttaatccacc aaaacttgga ccggctcgtg tgattgctta
      361 tgatatgatg gcgatcactt atgcacttaa aatcattaag gaacagcaga tggagcgacc
      421 aattttctat atcttgggaa ataccatagg tggcttcata gtaccttttg cgaaacagat
      481 tcactctgtt ggaggtacct tgtttgtcaa tccagatggt ttggaatgga aacgtgccaa
      541 gtgggctaag ccagtccaaa ggtatctaaa attttctgaa aaaatgatgg tcaaatatgc
      601 tgatttgatt attgctgata atgagggaat tgaggattat attcaatctg aatactcggc
      661 taccaataca cgctttattg cctatggtac agatttaagt ccgactaagt taacaaaaga
      721 atctgaacaa gttcgccaat attttacaaa ttggaacatt aaagagaaag aattttatct
      781 gattatcggt cgttttgtac ccgagaataa ctatgtgaca gcaattaagg agtttatggc
      841 aagcaacacg agacgtgatt tagtcatcgt tgcaaaccat gaaggctctg cctattttca
      901 aaaattgaaa gatgagacag gctttatatc cgataagcgg attaagttcg taggaacagt
      961 atatgaccaa gaactactta agtatctgcg ccaagaatgt cgagcttata ttcatggaca
     1021 tgaagttgga ggaaccaatc ctagtctcct agaagcgctg gcgcaaacca atgaaaattt
     1081 ggttttaggt gttgatttta accgcaaagt tgccctagaa ggtgctcggt attggagtaa
     1141 ggagtcaggt agcctgagtc aattaattaa ccaaattgat gagaatgctg aagcggtgga
     1201 attaggagaa attgcgaaaa aacacatgaa ggatgaatac acctgggaaa aaatcgtcca
     1261 agagtacgag gaattatttt tacaatgaaa gtaaacatac tcatgtcgac ctacaatggt
     1321 caacagttct tagctgagca aattcgctct attcaagaac agacttacac agactggaca
     1381 ctgttcattc gtgatgatgg ttccagtgat cgaactagag agattattaa agattttgtt
     1441 gaacaagatc agcgaattca cttcatagat gttgagacag acgaaaatct cggtgtcatc
     1501 aaaagttttc ataggctaat tcattatgat actgctgatt tctatttttt cagtgaccaa
     1561 gatgatgttt ggctaccaaa taaactggaa gttagcctga aaaaagcaca agcctatcca
     1621 acaaatttgc cattgatggt ttatatggac ctcaaagttg tcaatcaaaa tctagaagtt
     1681 atggcagaaa gcatggtaaa atctcaatct catcatgcca atacggaatt ggtgcaagag
     1741 ttgactgaga atactgtaac tggcggtgtc gccatgatta atcatcatct tgctgaaatg
     1801 tggcaagtaa ctgaggatat tttgatgcat gactggtatt tagctttgtt ggcctcggct
     1861 ttgggtaaac tggtttttat tgacaagcct ggcgaactct atcgccagca tgcggataat
     1921 gttttgggag ctagaaccct ttctaaacga tttaaaaaat ggattcgtcc tcacatttta
     1981 tttgcagttt attgggactt gattaaaaat agtcaaaaac aagcccggca tttattacaa
     2041 atgccccttt ctcaatcaaa tagagaatta atagaagcct ttgtaaccat tatggataaa
     2101 ccaatgcttg aacgattcag gattttgaga aaatatggtt taagaaaaaa caaaaccttt
     2161 catacattag tattcacaac actgattgtg acaaaatttg catataagga ataacaatga
     2221 atttattaaa taaagaaaat caaatactct taagagagat gattaaaaca gattttaaat
     2281 tacgttacca aggttctctc attgggcatc tttggtcgat tttgaaaccg atgttacttt
     2341 ttacaattat gtatttagta tttgttcgtt ttttgcgctt tgatgatgga actcctcatt
     2401 atgcagtagg gttattatta ggtatggtaa cctggaactt ttttacagaa gcaactaata
     2461 tggggatgat gtctattgtt tcacgcggag atttattgcg gaagttgaat ttttctaaag
     2521 aaattattgt attttcttca gttgcaggag ctgcaattaa ttacgccatt aacttaatgg
     2581 ttgtgtttgt atttgctgct attaacgggg ttccctttac ttggtccatt ctaattataa
     2641 tcccattgtt ttttgaattg gcattactag caacaggtat agctttcatt ttatcctctt
     2701 tatttgtaag atttagagat attggtccaa tttgggaagt gttcttacag gctggattgt
     2761 atgcaacacc aattatttat tcactaacat tcattttaca gcgtgggcaa gtaacatttg
     2821 ctaaattgat gatgttgaac cctgttgcac aaattattca agacttgaga cattttattg
     2881 tttattctgg gaatactaca gttagtgaat tgattgaaaa tcctgttatt gtggca
//
LOCUS       DBNFEG010000195         2901 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.196, whole genome shotgun sequence.
ACCESSION   DBNFEG010000195 DBNFEG010000000
VERSION     DBNFEG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.196"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            171..806
                     /locus_tag="AC1PNG_001951"
     CDS             171..806
                     /locus_tag="AC1PNG_001951"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702416.1"
                     /translation="MNHPKFTLRKLSIGLVSLGAGVALSGTLYPKNTVTVYAETPTLE
                     KTSETDSTSLIRSTAVVEDPEVAQANENKEGEVVDSSSTLDLPKSNAESASPEALTNE
                     NASESEVQVAENTSSSEEKATEKEERTVQYEEKEVDDYSTKVEKPTETVSSTVDKTSK
                     ELFTVSRTAEVDQEGVVTVTTQIVPKEIDKGAEIVVLVDTYKKWMKMLKNS"
     gene            1002..2864
                     /locus_tag="AC1PNG_001952"
     CDS             1002..2864
                     /locus_tag="AC1PNG_001952"
                     /inference="COORDINATES: protein motif:HMM:NF040483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum opacification factor"
                     /protein_id="HIP6702417.1"
                     /translation="MQGAIHEARRILESENSGKRKHIVLLSQGESTFSYDLSDDVKSK
                     PTYKTIEENKVVHTNPLLPWPFTFDVTVRRANLLDDAKSLISFLNKVGITRFDKALDD
                     VSTIAGITKLIGIIAKDPLDYINLVDVDSSQLEEKNFNYEKQIGEGYHFRSFYERKVE
                     PLTFRNQIVSKIKHNLTTELKKAELPTNTLSYIAEKLGLTNTSEKAIDLFANSLVDYI
                     FYGRKNIFNNHNLSAQAEAKIATKNGIHVYAVDVTKDKAGEEKNKNKFDEYLKKMSGD
                     DKKFLSVDENVAEKFKDVLTKVTLVDTLEDNVEIVKEPKDPKVSIKKNSSTFWLSWIT
                     SSKTTITWNLDKEDLIKAYQTNQPLSLTYQLQLKDKKKKDSQKLVSTEISHEINDKVG
                     KKVYSSLKVSLNHKTKKVSVPVPIEPQVPDKPIDMPKVENETPKGTPETPMQEKPNVP
                     EATLPMQPESPKETGKPGNNVEPESLKPIEDSTDDSANKPDSTIPKVTDTPDLSRPMK
                     PESTEEIDKAENSDTPDSSKTIKDSDNSAPISTIKDKVNAETVANQVELAMNQKSRLS
                     ENMEKVSSVNRSNEKSHTLPSTGQADSPIYTLTALSLLVGAQVLYRTKRKQESE"
BASE COUNT         1058 a          513 c          554 g          776 t
ORIGIN      
        1 accttatgaa tacacaaggt gcccattctt tttccaaaag ctctacgctt gttgaacaaa
       61 attatactaa agttatttac cattgacatc catacttcct tttttgaaaa tacctaaaga
      121 agggtgaaaa tacattctaa atttatagag aacaaggaga aggtatcata atgaatcatc
      181 ctaaattcac tttgcggaag ctatcaattg gacttgtttc gctaggagca ggggtagctt
      241 tatcgggaac actctatccc aaaaatactg tgacagtata tgcagaaaca cctactttag
      301 aaaaaacttc ggagacagat agcacttctc tgattaggtc tactgctgtc gttgaagacc
      361 ctgaagttgc ccaagctaat gaaaacaagg aaggagaggt ggttgactcc tcttctactt
      421 tggacttacc aaaatcgaat gcagaatctg cttctcctga ggcattgacg aatgaaaacg
      481 cttcagaatc agaagtgcaa gttgctgaaa acactagttc ttctgaagaa aaagcaacag
      541 aaaaagaaga acgaaccgtt cagtatgagg agaaggaagt agacgactat tctactaagg
      601 ttgaaaaacc aacggaaaca gtatctagta cagtggacaa aacttctaaa gagcttttta
      661 cagtttctcg aacagcagaa gtagatcaag agggagttgt cacagtaaca actcaaattg
      721 tgccgaaaga aattgacaag ggtgcagaaa tagttgtctt ggtggataca tataaaaaat
      781 ggatgaagat gctaaaaaac agctaaagat aacattataa agcttgtcga aaagatgact
      841 gacccaacca atgatcataa ttctcgtaac tctgtgcgtg taatcggttt caaccgcaag
      901 ttatctgaag ctcaagaagt taccatacca gtgctaatgt agaaactacg gttgagaagc
      961 ttttcactga tgctgaacaa aattacaatt ggggcgtaga tatgcaggga gcaatccatg
     1021 aagcacgtcg cattttagaa agcgagaact cggggaaacg caaacatatt gtacttctgt
     1081 cacaagggga gtctacattt agttatgatt tgtcggatga tgtaaaatca aaacctactt
     1141 ataagaccat tgaggaaaat aaggttgtac atacaaatcc actcttgcct tggccattta
     1201 catttgatgt gactgttcgc agggcaaact tgttggatga tgcaaagagt ctaataagtt
     1261 ttttgaacaa agttgggatt acacgtttcg ataaagcttt agatgatgta tccactatag
     1321 caggaataac aaagcttata ggaataattg caaaagatcc gttagactat attaatttgg
     1381 ttgatgtaga cagttcgcag ttagaagaaa aaaatttcaa ttacgagaaa caaattggtg
     1441 aaggttatca tttccgtagc ttttatgaac gtaaagttga gccgttgaca tttagaaatc
     1501 aaattgttag caagataaaa cataatttaa caactgagtt aaaaaaagct gagttaccta
     1561 caaacactct tagctatatc gctgaaaagc tcggtttaac gaatacttct gaaaaagcaa
     1621 ttgatttatt tgcgaattcc ctcgttgatt atattttcta cggtagaaaa aatattttta
     1681 acaaccacaa cctctctgct caagcagagg ccaagattgc tactaaaaat ggtattcatg
     1741 tctacgctgt tgatgtgacc aaagacaaag ctggcgaaga aaagaataaa aataaatttg
     1801 atgaatactt gaaaaaaatg tctggggatg ataagaagtt tttaagtgtt gatgagaatg
     1861 ttgcagaaaa atttaaagat gtactgacta aagtgacatt ggtagatact cttgaagata
     1921 atgttgaaat tgttaaagaa cccaaagacc ctaaagtaag tattaaaaag aattcttcaa
     1981 cgttctggct gtcatggatt actagctcga aaacaactat tacttggaac ttagataaag
     2041 aagacttaat caaggcttat cagactaatc aaccactttc tttaacttat cagttacaac
     2101 tgaaagataa aaagaagaaa gattcccaaa aacttgtaag tacagagatt agccatgaga
     2161 ttaatgataa agttgggaag aaagtatact catccctaaa agtgtctctt aaccataaaa
     2221 ctaagaaagt ttcggttcct gttcctattg agccacaagt tcctgacaag ccaattgata
     2281 tgccaaaagt agagaatgaa acgcctaagg ggactcctga aacacctatg caggaaaaac
     2341 caaatgttcc agaagcaact cttccaatgc aaccagagtc acctaaagag accggaaaac
     2401 caggcaataa tgttgagcca gaatcactta agcctatcga agactcaacc gatgattcag
     2461 caaataaacc agactcaaca attccaaaag taacggatac cccagatttg tctcgcccaa
     2521 tgaaaccaga gtcaactgaa gagattgata aagcagaaaa tagtgatact ccagattctt
     2581 ctaagactat taaagattca gataactctg ctccaatttc tactatcaaa gataaagtga
     2641 atgccgaaac ggtggcaaat caggtagagc ttgctatgaa tcagaaatca cgtctttctg
     2701 aaaatatgga gaaagtttct agcgtgaatc gttcaaacga gaaatcacat acccttccat
     2761 ctactggaca agctgattct ccaatctata cgttaactgc cctctctctt cttgtaggag
     2821 ctcaggtact ttatcgtaca aaacgtaagc aagaatcgga gtaaatgttg gtcgtgtaat
     2881 ttctacttgt atatacatat g
//
LOCUS       DBNFEG010000196         2885 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.197, whole genome shotgun sequence.
ACCESSION   DBNFEG010000196 DBNFEG010000000
VERSION     DBNFEG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.197"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            18..392
                     /locus_tag="AC1PNG_001953"
     CDS             18..392
                     /locus_tag="AC1PNG_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIP6702418.1"
                     /translation="MAWKFDNNIPIYIQISNTIKLQIVTNQLKSGDKLPTVRDLADIA
                     GVNPNTVQRALSDLETEGFVYSVRTTGRFVTDNQELITQTRLHLAQKELENFVNNMLE
                     LGFRHDELTSQLDDYLKGVTYE"
     gene            385..1092
                     /locus_tag="AC1PNG_001954"
     CDS             385..1092
                     /locus_tag="AC1PNG_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6702419.1"
                     /translation="MSNAYTLVELQQVSKSYGGAVALNNVNLKLTAGKIIGLLGPNGS
                     GKTTMIKLINGLLQPEYGQVLINGRTPSPETKAIVSYLPDTTYLDEHMKVLEAITFFE
                     DFYADFDKERALHLLQDLGIDLNSRMKHLSKGNKEKVQLILVMSRQAQLYVLDEPIGG
                     VDPAARDYILKTIVNNYSPTASVIISTHLISDVEQILDEVIFLHYGSVIRHGNVDDLR
                     IESGESIDELFRQDFKA"
     gene            1109..1891
                     /locus_tag="AC1PNG_001955"
     CDS             1109..1891
                     /locus_tag="AC1PNG_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6702420.1"
                     /translation="MFKKLLKYEFQSVGKWYLGIYIAAVVLSAVLGFWLQALALRTQA
                     GNTEPGGGEMVLLGTSFMTFGILIAALFLSTFVLVINRFRKNVYGRQGYLTMTLPVSS
                     YQIILSKLLASIVWYILAGITCLLSIGIILAVILAASEEAIMPEIQTFVQAIDWTVLF
                     SQIFYYLIEITTGILLIYFSISVGQLFKDHRFLFAILTYIGILIVTGVFSTFFVLSNL
                     GTAYSTPFSLYPSPVLALVNIILAFAYYFGTHFIMTKKLNLQ"
     gene            complement(1935..>2885)
                     /gene="add"
                     /locus_tag="AC1PNG_001956"
     CDS             complement(1935..>2885)
                     /gene="add"
                     /locus_tag="AC1PNG_001956"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002962799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HIP6702421.1"
                     /translation="GSLSLGVIRQLAQMAKITIPAEDEALRKLVSVHGKVDSLMAYLK
                     LFDFVRPLLQTATALELAAYDLVRQAASDKVIYIEVRFAPELSTDQDLTILEAVSAVL
                     VGLNRGQEDFGVLAKLLVCGLKQTNTNQTKELFSAIADLAPKGLVGFDFAGNEADYPT
                     EELRDIIQFTQSLGYPMTFHAGECGCVINVIQALELGIRRIGHGTALTRNPEAIQAFV
                     NSGATLEMCLTSNLQTGAADSIEYFPYRELVEAGANITINTDNRTVSNTTLNREYQLF
                     VEYFGTSKADFYRFNQNAIQASFASEGEKKVLLELLDQQY"
BASE COUNT          850 a          586 c          535 g          914 t
ORIGIN      
        1 ctagaaagga aacgattatg gcttggaaat ttgataataa catccccatc tatattcaaa
       61 tcagcaatac tattaaattg cagattgtga ccaatcaatt aaaatctgga gataaacttc
      121 ccactgttcg tgacttagcc gacattgctg gtgtcaatcc aaataccgtt cagcgcgctt
      181 tgtctgattt agaaacagaa ggatttgttt actcagtacg cacaacaggc cgttttgtaa
      241 cggataatca ggaattgatt acacaaactc gcctacattt agcccaaaag gaactggaga
      301 acttcgtgaa caatatgtta gaattgggct ttagacacga tgaactcact agccagttag
      361 atgattattt gaaaggagtt acttatgagt aacgcatata ctttggttga gctacagcaa
      421 gtttcaaaat cttatggtgg agctgtcgcc ctaaataatg tcaatttgaa gttgactgct
      481 ggaaaaatca taggactttt gggaccaaat ggttcgggta aaacaaccat gattaaattg
      541 atcaacggtc tgttgcagcc agaatatggt caagtattga tcaatggtcg cacaccatcg
      601 ccagaaacca aggcgattgt atcttatctc ccagatacca cttatcttga tgaacatatg
      661 aaagtgctcg aagcaattac attttttgaa gatttttacg ctgattttga taaggagcgt
      721 gctctccatc ttttacaaga tttgggcatt gatttaaaca gtcgtatgaa acatctctct
      781 aaggggaata aggaaaaagt ccagctcatt ctggtgatga gccgtcaagc acagctgtat
      841 gttttagatg agccaattgg tggtgttgac cctgccgccc gagactacat tttaaaaacc
      901 atcgtcaaca actactctcc gactgcttct gttattatct ctactcactt aatttcagat
      961 gttgagcaaa ttttggacga ggttattttc ctacactacg gaagtgttat tcgccatggg
     1021 aatgtagatg acttgcgaat tgaaagcgga gaatctattg acgaactctt ccgccaagat
     1081 tttaaggctt agttagaagg agattgctat gtttaaaaaa ttattgaaat acgaatttca
     1141 atccgtaggt aaatggtatt tgggaattta tattgcagct gtggtcctat cagcagtact
     1201 tggtttttgg ctacaagcgt tggccctccg tacacaggct ggaaatactg aacctggcgg
     1261 tggagagatg gtcttattgg gaacatcctt tatgactttt ggtattttga ttgccgcact
     1321 cttcttgtca acatttgttc tagtaatcaa ccgttttaga aaaaatgttt atggccgaca
     1381 aggttatctc actatgaccc tgccggtatc tagctatcag atcatcttaa gcaaactact
     1441 tgcctctatt gtttggtata tcttggcagg tattacttgc ttactttcaa ttggaatcat
     1501 ccttgcagtc attctagcag caagcgaaga agctattatg ccagaaatac aaacttttgt
     1561 tcaagctatc gactggacag ttctattcag tcagattttc tattatttaa ttgaaattac
     1621 cactggtatc cttttgatct acttctctat atctgtcggt caattattca aggatcatcg
     1681 cttcctcttt gcaatcttga cttatatcgg cattctcatc gttactggag tatttagcac
     1741 tttctttgtc ctaagtaacc taggaacagc ttattctaca ccattttctc tctatccaag
     1801 tccagtctta gcattagtca atattatcct agcttttgct tactactttg gaacccactt
     1861 cattatgaca aagaaattaa atctacaata aaaagctctt tactggtcta atcgaccggt
     1921 atagagcttt tttcttaata ctgttggtct aggagttcca acaaaacttt cttttctcct
     1981 tcgctggcaa aacttgcctg aatagcattt tgattgaagc gataaaaatc agccttgcta
     2041 gtcccaaaat attctacaaa taactgatat tctctattaa gtgtagtatt agaaacggta
     2101 cgattatctg tgttgattgt gatgtttgct cctgcctcaa ctagctcacg atatggaaaa
     2161 tattcaatcg aatcagctgc accagtttga agattcgatg ttagacacat ttcaagtgtt
     2221 gctccgctat tgacaaaagc ttggatagct tcaggattgc gagtaagtgc tgtgccatga
     2281 ccaatccttc taatcccaag ttcaagagct tgaatcacat tgattacaca tccacattct
     2341 ccggcatgga aagtcatggg ataacctaaa gactgagtaa attgaataat atcccgaagt
     2401 tcttccgtag gatagtctgc ttcatttcct gcgaaatcaa atcccacaag tccttttggt
     2461 gccaaatcag caatagccga aaagagttcc ttcgtttgat tcgtattcgt ttgctttaaa
     2521 ccacaaacca gaagtttagc aagcactcca aaatcttcct gtcccctgtt tagtccaact
     2581 aaaactgctg agactgcttc taaaatagtc aagtcttgat ccgtcgacag ttcaggtgca
     2641 aaacgaacct caatgtaaat gaccttatca cttgcagctt gtcgtacaag atcataggct
     2701 gccaattcta aagctgttgc agtttgtaag agtgggcgaa caaaatcaaa taactttaaa
     2761 taagccatta aactatcaac ttttccatga acactaacta attttcttaa tgcttcatct
     2821 tctgcaggaa tagtaatctt agccatctgt gccaattgtc gaatgactcc gagggacaat
     2881 gagcc
//
LOCUS       DBNFEG010000197         2847 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.198, whole genome shotgun sequence.
ACCESSION   DBNFEG010000197 DBNFEG010000000
VERSION     DBNFEG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.198"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..760
                     /locus_tag="AC1PNG_001957"
     CDS             <1..760
                     /locus_tag="AC1PNG_001957"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIP6702422.1"
                     /translation="YFDLKQLVSDFELLGIDVGVDINSFGFTVLKNKKIQMLGDHSIV
                     TFSTNFYKPSHILMKRLLDILGAVVGLIICGIVSILLIPIIRRDGGPAIFAQKRVGQN
                     GRIFTFYKFRSMFVDAEVRKKELMAQNQMQGGMFKMDNDPRITPIGHFIRKTSLDELP
                     QFYNVLIGDMSLVGTRPPTVDEFEKYTPSQKRRLSFKPGITGLWQVSGRSDITDFNEV
                     VRLDLTYIDNWTIWSDIKILLKTVKVVLLREGSK"
     gene            799..1968
                     /gene="cps2T"
                     /locus_tag="AC1PNG_001958"
                     /gene_synonym="wchF"
     CDS             799..1968
                     /gene="cps2T"
                     /locus_tag="AC1PNG_001958"
                     /gene_synonym="wchF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta 1-4 rhamnosyltransferase Cps2T"
                     /protein_id="HIP6702423.1"
                     /translation="MRTVYIIGSKGIPAKYGGFETFVEKLTEYQKDKSINYFVACTRE
                     NSAKSGITGEVFEHNGATCFNIDVPNIGSAKAILYDIMALKKSIEIAKDRNDTSPIFY
                     ILACRIGPFIYLFKKQIESIGGQLFVNPDGHEWLREKWSYPVRQYWKFSESLMLKYAD
                     LLICDSKNIEKYIHEDYRKYAPETSYIAYGTDLDKSRLSPTDSVVREWYKEKEISEND
                     YCLVVGRFVPENNYEVMIREFMKSYSRKDFVLITNVEHNSFYEKLKKETGFDKDKRIK
                     FVGTVYNQELLKYIRENAFAYFHGHEVGGTNPSLLEALSSTKLNLLLDVGFNREVGEE
                     GAKYWNKDNLHRVIDSCEQLSQEQINDMDSLSTKQVKERFSWDFIVDEYEKLFKG"
     gene            1972..>2847
                     /locus_tag="AC1PNG_001959"
     CDS             1972..>2847
                     /locus_tag="AC1PNG_001959"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIP6702424.1"
                     /translation="MKKILYLHAGAELYGADKVLLELIKGLDKNEFEAHVILPNDGVL
                     VPALREVGAQVEVINYPILRRKYFNPKGIFDYFISYHHYSKQIAQYAIENKVDIIHNN
                     TTAVLEGIYLKRKLKLPLLWHVHEIIVKPKFISDSINFLMGRFADKIVTVSQAVANHI
                     KQSPHIKDDQISVIYNGVDNKVYYQSDARSVRERFDIDEEALVIGMVGRVNAWKGQGD
                     FLEAVAPILEQNPKAIAFIAGSAFEGEEWRVVELEKKISQLKVSSQVRRIDYYANTTE
                     LYNMFDIFVLPSTNPD"
BASE COUNT          967 a          400 c          582 g          898 t
ORIGIN      
        1 atattttgac ttaaagcaat tagtttcaga ctttgagttg ttaggtattg atgtaggcgt
       61 tgatattaat tcattcggtt ttactgtgtt gaagaataaa aaaatccaaa tgctaggtga
      121 ccatagcatc gtcacttttt ccacaaattt ttataagcct agtcacatct tgatgaaacg
      181 acttttagat atacttggag cagtagtcgg gttaattatt tgtggtatag tttctatttt
      241 gttaattcca attattcgta gagatggtgg accagccatt tttgctcaga aacgagttgg
      301 acagaatgga cgcatattta cattctacaa gtttcgttcg atgtttgttg atgccgaggt
      361 acgtaagaaa gaattaatgg ctcaaaacca gatgcaaggt gggatgttca aaatggacaa
      421 cgatcctaga attactccaa ttggacactt catacgaaaa acaagtttag atgagttacc
      481 acaattttat aatgttctaa ttggagatat gagtctagtc ggtacccgtc cacctacagt
      541 tgatgaattt gaaaaatata ctcctagtca aaagagaaga ttgagtttta aaccagggat
      601 tacaggtctt tggcaagtga gcggaagaag tgatatcaca gattttaatg aagtcgttag
      661 gctggaccta acatacattg ataattggac catctggtca gacattaaga ttttattgaa
      721 gacagtgaaa gttgtattgt tgagagaggg aagtaagtaa gactccttta aaacaaagaa
      781 tagtaagtag taggggatat gagaacagtt tatattattg gttcaaaagg aataccagca
      841 aagtatggtg gtttcgagac tttcgtagaa aaattaactg agtatcagaa agataaatca
      901 attaattatt ttgttgcatg tacaagagaa aattcagcaa aatcaggtat tacaggagaa
      961 gtttttgaac ataatggagc aacatgtttt aatattgatg tgccaaatat tggttcagca
     1021 aaagccattc tttatgatat tatggctctc aagaaatcta ttgaaattgc caaagataga
     1081 aatgatacct ctccaatttt ctacattctt gcttgtcgga ttggtccttt catttatctt
     1141 tttaagaagc agattgaatc aattggaggt caacttttcg taaacccaga cggtcatgaa
     1201 tggctacgtg aaaagtggag ttatcccgtc cgacagtatt ggaaattttc tgagagtttg
     1261 atgttaaaat acgctgattt actaatttgt gatagcaaaa acattgaaaa atatattcat
     1321 gaagattatc gaaaatatgc tcctgaaaca tcttatattg cttatggaac agacttagat
     1381 aaatcacgcc tttctccgac agatagtgta gtacgtgagt ggtataagga gaaggaaatt
     1441 tcagaaaatg attactgttt ggttgttgga cgatttgtgc ctgaaaataa ctatgaagta
     1501 atgattcgag agtttatgaa atcatattca agaaaagatt ttgttttgat aacgaatgta
     1561 gagcataatt ccttttatga gaaattgaaa aaagaaacag ggttcgataa agataagcgt
     1621 ataaagtttg ttggaacagt ctataatcag gagctgttaa aatatattcg tgaaaatgca
     1681 tttgcttatt ttcatggtca cgaggttgga ggaacgaacc catctttact tgaagcactt
     1741 tcttctacta aactaaatct tcttctagat gtgggcttta atagagaagt aggggaagaa
     1801 ggagcgaaat actggaataa agataatctt cacagagtta ttgacagttg tgagcaatta
     1861 tcacaagaac aaattaatga tatggatagt ttatcaacaa aacaagtcaa agaaagattt
     1921 tcttgggatt ttattgttga tgagtatgag aagttgttta aaggataagt tatgaaaaag
     1981 attctatatc tccatgctgg agcagaatta tatggggcag ataaggttct cttggaactt
     2041 ataaaaggct tagataagaa tgaatttgaa gcgcatgtta tcctacctaa tgatggagtc
     2101 ctagtgccag cattaagaga agttggtgcg caagttgaag ttattaacta tccaattcta
     2161 cgtaggaaat attttaatcc aaaagggatt tttgactact tcatatcata tcatcactat
     2221 tctaaacaga ttgctcaata tgccatagaa aataaggttg acataattca caataatact
     2281 accgctgtct tagaaggcat ttatctgaag cgaaaactca aattaccttt gttgtggcat
     2341 gttcatgaga ttattgtcaa acctaaattc atctctgatt cgatcaattt tttaatgggg
     2401 cgttttgctg ataagattgt gacagtttca caggctgtgg caaaccatat aaaacaatca
     2461 cctcatatca aagatgacca aatcagtgta atctacaatg gggtagataa taaagtgtat
     2521 tatcagtccg atgctcggtc tgttcgagaa agatttgaca ttgacgaaga ggctcttgtc
     2581 attggtatgg tcggtcgagt caatgcgtgg aaaggacaag gagatttttt agaagcagtt
     2641 gctcctatac tcgaacagaa tccaaaagct atcgccttta tagcaggaag tgcttttgaa
     2701 ggagaagagt ggcgagtagt agaattagaa aagaagattt ctcaattaaa ggtctcttct
     2761 caagtcagac gaattgatta ttatgcaaat accactgaat tatataatat gtttgatatt
     2821 tttgtacttc caagtactaa tccagac
//
LOCUS       DBNFEG010000198         2839 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.199, whole genome shotgun sequence.
ACCESSION   DBNFEG010000198 DBNFEG010000000
VERSION     DBNFEG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.199"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(258..1085)
                     /locus_tag="AC1PNG_001960"
     CDS             complement(258..1085)
                     /locus_tag="AC1PNG_001960"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIP6702425.1"
                     /translation="MKLISWNIDSLNAALTAESPRALLSRAVIDTLVTEDADIIAIQE
                     TKLSDKGPTKKHLEILESYFPGYANTWRSSVEPARKGYAGTLFLYKNHLSPTITFPEI
                     GAPTTMDSEGRIITLEFDDFYVTQVYTPNAGDGLKRLADRQIWDVQYADYLAKLDSHK
                     PVLATGDYNVAHKEIDLANPASNRQSPGFTDEERQGFTNLLAKGFTDTFRHLHGDVLN
                     AYTWWAQRSRTSKINNTGWRIDYWLVSDRIADKVSKSDMIDSGARQDHTPIVMEISF"
     gene            1241..>2839
                     /locus_tag="AC1PNG_001961"
     CDS             1241..>2839
                     /locus_tag="AC1PNG_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702426.1"
                     /translation="MKRKLFVFAWLALVLEMLIYYWYQLPALNILSVDFWIFFLQSVG
                     LVWLILSMFSSKGAFQKVTKISGRQRQVDTYQIKTSQLPAYLKWLGRIWVLVVLGLLG
                     LGLINSRVFRAKDYAAVISVKDADFKADFPETDISKLALLDRASAEKIGDTYLGTIDK
                     VSQFGISDDYRQITIGQQPFRVSPLEYKNFWKWLSNHQDGIGYYVKVNQTTGKAELAK
                     LSKAMHYSDSEFLLNDTLRHLRLQYPTTIFGKPSFEVDDQGNPYYIATIYEPKFGLSS
                     NDPIGAIVLDAVTGESKKYSLEDIPEWVDRVYSANNVISRVDDHYTYQNGFWNTIFSQ
                     TGVKNTTDSYNYISIGSDIYLYTGITSATADSSNLGFILVNMRTREITNYKLASATET
                     AAQESAEGEVQEKGYKATAPSLVKLADTAYYLVSLKDDAGLVKSYALVDAEDYQQVTV
                     NNDIALLISQVTGTDASSLSGLTVTENGEVGEQTEVISGKVEALASQIIGGATIYYIE
                     SEGKIYKVKATEDSTDRLPFIKVGD"
BASE COUNT          829 a          533 c          627 g          850 t
ORIGIN      
        1 aaaatgacac cgttttcaaa ttgagcggat taaaacaagg tagagaggta gagtgataag
       61 aaataaaggc tcaaaaccct tgcaatagac ctacgggttt taagccttta atatctgtta
      121 tctgataatt gaaatcaggt tatactcttc gaaaatcaaa atcagacgtt gttgacttga
      181 tttgatgaac ttcagttcta tctgcatctg cgtcgcctag tctgattttg attttcattg
      241 agtattaata tccactgtta gaatgaaatc tccatcacaa ttggtgtatg gtcctgacga
      301 gcaccggagt caatcatatc tgacttactt actttgtcgg cgatgcggtc gctgacaagc
      361 cagtaatcaa ttctccagcc tgtgttgttg attttactgg tgcggctacg ttgtgcccac
      421 caagtatagg cgttgagaac atcaccgtgc aagtggcgga aggtatctgt aaaacctttt
      481 gctagaagat ttgtaaaacc ttgacgttct tcatctgtaa accctggaga ttgacggtta
      541 cttgcgggat tagcaaggtc aatttccttg tgagcaacgt tgtagtcacc tgttgcaaga
      601 acaggtttgt ggctatcaag ttttgctaag tagtcagcat actgtacatc ccagatttga
      661 cggtcagcaa gccgtttcaa gccatcacca gcatttggcg tataaacttg tgtgacatag
      721 aagtcatcaa attctagcgt gatgatacgc ccttcagaat ccatggttgt tggagcacca
      781 atctcaggga aggttatggt tggacttaga tggtttttgt agaggaagag tgtaccagca
      841 tagcctttgc gagctggttc tacagatgaa cgccaggtat tggcatatcc agggaagtag
      901 ctttccaaaa tttctagatg ctttttagtc ggtcccttat ctgacaattt tgtttcctga
      961 atggcgataa tatcagcgtc ttctgtcaca agtgtatcga tgactgcacg tgataatagg
     1021 gcgcgaggag actctgcagt caatgcagcg ttaagagaat caatattcca agaaatgagt
     1081 ttcataattt tccttttcta gtctgttact gttttacatt ataccaaaaa taaaagttgc
     1141 aggcgaatat ggaaacttga aaaaaatttt ataattcttg tattataaca ttaaaattgt
     1201 gatacaatag aactaataaa tctgtgtcaa ggaggttatt atgaaaagaa aattatttgt
     1261 ttttgcctgg ttagccctag ttttggaaat gctaatctac tactggtacc agttgcctgc
     1321 tttaaatatt ttgagtgtgg acttctggat atttttcctt caatctgtcg gattggtctg
     1381 gctcatactt tccatgttca gctccaaagg tgcttttcaa aaggtgacca agatttccgg
     1441 tcgccagaga caagtagata cctatcagat taaaacctcg caactgccag cttacttaaa
     1501 atggttggga cgaatttggg tattggttgt cctgggtttg ttggggctgg ggcttatcaa
     1561 ctcacgtgta tttcgtgcta aggattacgc tgctgtcata agcgttaagg atgcggattt
     1621 taaagcagat tttcctgaaa cagacatatc caaattggcc ctcctagacc gcgcctcggc
     1681 cgaaaaaatc ggtgatactt atctaggtac cattgacaag gtctcccagt ttggcatttc
     1741 ggatgattat cgccagatta ctatcggtca gcaaccgttc cgtgtatcgc ctttggaata
     1801 taagaatttt tggaaatggc tgagcaacca tcaagatggg attggttact acgtgaaggt
     1861 taatcagaca acagggaagg cagaacttgc caagttgagc aaagccatgc actattcaga
     1921 ttccgaattt ttgctgaatg ataccttacg tcatcttcgc ttgcaatacc cgacaactat
     1981 ttttgggaaa ccatccttcg aagtggatga ccaaggaaat ccatattata ttgcaacgat
     2041 ttacgagcca aaatttggac tttcatcaaa tgatccgatt ggtgctattg tattggatgc
     2101 agtaacaggg gaaagtaaga agtatagtct ggaagatatt ccagagtggg tagaccgtgt
     2161 ttattcggca aacaatgtta tcagccgtgt agatgaccac tatacttatc aaaatggttt
     2221 ctggaatacc attttcagtc aaactggtgt gaaaaataca acagacagct ataattatat
     2281 ttctatcggc tcagatattt atctctacac aggtatcacc tcagcaactg ctgattcatc
     2341 caaccttgga tttattcttg tcaatatgag aactcgtgaa attaccaact acaaattggc
     2401 ttcggcgacg gaaacagcag cgcaagagtc cgcagaaggt gaagtgcagg aaaaaggcta
     2461 taaagccact gctccaagcc tggttaagtt ggcagataca gcctactatt tagtatcctt
     2521 gaaagatgat gccggcttgg ttaaatcata cgctcttgtt gatgccgaag attatcaaca
     2581 ggttactgtt aacaacgata ttgcgctctt aatttctcaa gtcacaggaa cggatgcttc
     2641 tagcctttca ggattgacgg tgactgaaaa tggagaagtt ggtgaacaga ctgaggttat
     2701 cagtggaaaa gtagaagctt tagcgagtca aataattggc ggtgcgacaa tctactatat
     2761 tgagtctgag ggcaagattt acaaagtcaa agcaacggaa gatagtacag atcgattacc
     2821 atttatcaaa gtaggagat
//
LOCUS       DBNFEG010000199         2803 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.200, whole genome shotgun sequence.
ACCESSION   DBNFEG010000199 DBNFEG010000000
VERSION     DBNFEG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.200"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(27..1268)
                     /gene="dltB"
                     /locus_tag="AC1PNG_001962"
     CDS             complement(27..1268)
                     /gene="dltB"
                     /locus_tag="AC1PNG_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HIP6702427.1"
                     /translation="MMEFFKQLPQLEPYGNPLYFFYLVLALVPIFIGLFFKKRFPLYE
                     TGVSLAFIVLMFTGTKTMQLLSLLAYIIWQTMLIFFYKHYRQRANQSWVFYLIVCLAV
                     FPLTWVKLAPTFSQHGAIFGFLGISYLTFRSVGMVIEMRDGLLTEFSLGSFLRFLLFM
                     PTISSGPIDRYRRFTEDYKQIPERTELLNMLDQTMHYIMMGFLYKFILAYFIGHTLLE
                     PLKAVALDQGGWFNLPTIGVMYLYGFELFFDFAGYSMFAIGISNLMGIRCPINFDQPF
                     KSRDLKEFWNRWHISLSFWFRDFVFMRLVKTLLKHKVFKNRNTVSNVAYLLNMLLMGL
                     WHGVTWYYIAYGLFHALGLIINDAWIRKKKSINLARKKAGQEPLPDNRWTSFAGMFVT
                     FHTVMFSFLIFSGFLDKLWFK"
     gene            complement(1265..2800)
                     /gene="dltA"
                     /locus_tag="AC1PNG_001963"
     CDS             complement(1265..2800)
                     /gene="dltA"
                     /locus_tag="AC1PNG_001963"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HIP6702428.1"
                     /translation="MISSMLERVEHFATENPDYPVYHYLGQLYSYGDLKADSDSLAAH
                     IDSLDLPANSPVLVFGGQEYQMLAIFVTLTKAGHPYIPVDSHSALDRIEAILEIAEPS
                     LVFAVADFPLETSLPVLGLSEVEAIFEKKSTYQLMHPVSGDDTYYIIFTSGTTGKPKG
                     VQISHDNLLSFTNWMIRDKEFALPERPQMLAQPSYSFDLSVMYWAPTLALGGTLFALP
                     KELTLDFKTLFAAIQTLPFKIWTSTPSFVDMALLSREFDGEHLPDLTHFYFDGEELTV
                     KTAQKLRERFPKAVIVNAYGPTEATVALSAVAITDEMLVNYKRLPIGYAKPDSPTFIL
                     DEDGQVVPNGQQGEIVVSGPAVSKGYLNNPERTEAAFFQFDGLQAYHTGDLGVMTDEG
                     LLLYGGRMDFQIKYNGYRIELEEVAQHLNKSTYIEAAVAVPRYNADHKVQNLLAYVVP
                     KAGIMEQFDRKLELTKVIKQELKERMMDYMMPSKFIYRDSLPLTANGKIDIKGLIAEV
                     NAQ"
BASE COUNT          951 a          638 c          482 g          732 t
ORIGIN      
        1 cgatttatac aatcgttacc tattgtctac ttgaaccaca atttatctaa aaatcctgaa
       61 aatatcaaga aagagaacat gaccgtatgg aaggtcacaa acataccagc gaaactcgtc
      121 caacgattat ccggcaatgg ttcttgccca gctttttttc gagctaaatt aattgatttc
      181 ttcttacgaa tccaagcatc attgatgatc aaccccaaag catgaaagag accatatgca
      241 atataatacc atgttacacc atgccacaag cccatcaaga gcatatttaa cagataggca
      301 acattagata ccgtatttcg atttttgaaa accttatgtt tcaacaaagt cttaaccaga
      361 cgcatgaaga cgaaatcacg gaaccaaaaa gataagctga tatgccatcg attccaaaat
      421 tccttaagat cacgcgattt gaacggttga tcaaagttaa ttgggcagcg aatacccata
      481 agattggaaa tacctatggc aaacatggaa tagcctgcaa agtcaaagaa taattcaaaa
      541 ccataaagat acataactcc aatagtaggt aagttaaacc atccaccttg atccaaggct
      601 actgccttta aaggctccag taaagtatgc ccgataaaat aggctagaat aaacttatat
      661 aagaagccca tcataatata atgcatagtc tgatcgagca tatttaataa ctcagttcgc
      721 tcaggaattt gtttgtaatc ttccgtaaac cgtctataac gatcaatagg accactcgaa
      781 atagttggca taaatagcaa aaatcgcaaa aaacttccca aactaaattc cgttagtaaa
      841 ccgtcccgca tttcaatcac cattccgacc gaccgaaacg ttaaataaga aatacctaaa
      901 aaaccgaaaa ttgccccatg ctgactaaaa gtcggtgcca actttaccca agtcaacgga
      961 aaaacagcta aacacacgat taaatagaag acccagcttt gatttgcccg ttgacgataa
     1021 tgcttgtaga aaaaaatcaa cattgtctgc caaataatat aggccaataa agacagcaat
     1081 tgcattgtct ttgtacctgt aaacatcaag acaatgaagg ctaaactaac acctgtttca
     1141 tacaagggga aacgtttttt aaaaaagagt cctataaaaa ttggtactag cgccaagact
     1201 agatagaaaa agtacaatgg atttccataa ggctctaact gaggtagctg tttgaaaaac
     1261 tccatcattg agcattcacc tcagcgatca atcccttaat atcaatcttc ccatttgcag
     1321 tcaaaggaag actgtctcga taaataaact tcgaaggcat catataatcc atcatccgtt
     1381 cttttaactc ctgcttgatg accttggtta actccaactt tcgatcgaac tgctccataa
     1441 tccctgcttt agggacaaca taagctaaca gattttgcac tttatggtca gcattataac
     1501 gtggaacagc taccgcagcc tcaatatagg ttgatttatt taagtgttgg gcaacttctt
     1561 ccaactcgat acgataacca ttatatttaa tttggaaatc catacgccca ccatagagta
     1621 atagaccttc atctgtcatt acacccaaat ccccagtatg ataggcctgt aagccgtcaa
     1681 actggaaaaa tgccgcctcg gttctctcag gattattcaa atatccttta gagacagcag
     1741 gacctgatac aacaatttct ccctgttgac cattcggtac tacttgacca tcctcatcca
     1801 aaataaaagt tggcgagtct ggtttggcat agccaatcgg caaacgttta taattcacca
     1861 gcatctcatc agtgatggca actgctgata aggcgactgt agcttcggtc ggtccataag
     1921 cgttaacaat cacagctttt ggaaaacgtt cgcggagctt ctgagctgtt tttacagtta
     1981 actcctctcc gtcaaaatag aaatgcgtca aatctggtaa atgttcccca tcaaactcac
     2041 gagatagcaa agccatatct acaaatgatg gtgtcgaagt ccaaatctta aatggcaaag
     2101 tttgaatagc tgcaaataag gtcttaaaat ccaaagtcaa ctctttcggt agggcaaata
     2161 aggtcccacc aagggcaagc gtcggagccc aatacataac cgacaagtca aacgaatagg
     2221 aaggttgagc caacatttgc ggtcgctctg gtagcgcaaa ctccttatcc cttatcatcc
     2281 agttagtgaa gctgagcaaa ttgtcatgcg aaatttgcac cccttttggt ttacccgttg
     2341 tcccagaagt gaagataata tagtaggtat catctccact tacaggatgc atcaactgat
     2401 aagtcgactt cttctcaaaa atggcttcaa cttcagacaa accaagcact ggcaaacttg
     2461 tctctagcgg aaagtctgct accgcaaaca ccaaactcgg ctctgctatt tccagaatag
     2521 cttcaatccg atccaaagca gaatgactat ctacagggat atacggatgg ccagccttgg
     2581 ttaaggtaac aaagattgcc aacatctggt actcctgtcc gccaaaaaca aggacaggag
     2641 agtttgctgg taaatccaaa ctatcaatat gggctgctaa agaatctgaa tctgccttta
     2701 aatccccata actatacaac tgtcctagat aatggtaaac agggtagtca ggattttctg
     2761 tcgcaaaatg ctctacccgc tctaacatac tgctaatcat ttt
//
LOCUS       DBNFEG010000200         2783 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.201, whole genome shotgun sequence.
ACCESSION   DBNFEG010000200 DBNFEG010000000
VERSION     DBNFEG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.201"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..600
                     /locus_tag="AC1PNG_001964"
     CDS             <1..600
                     /locus_tag="AC1PNG_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702429.1"
                     /translation="DFVGKNFVDELPDDDSKIMIHFHTMILEIGSIIAALKIIKIVNN
                     EWHDRVVKSSVRYDIIRNVTYESLFYRVVFGITKIFDIREKNGIFKILSKLRHSTKDS
                     SLLSILNTIQDGIDKEQKNIDEIKLLRDKLLAHLDKEMVFSTERLDIGILYYYFEAIE
                     IKSIYTACIELYNTLYGDNQQQVELPKREIILKRFFLEE"
     gene            1396..2676
                     /locus_tag="AC1PNG_001965"
     CDS             1396..2676
                     /locus_tag="AC1PNG_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIP6702430.1"
                     /translation="MTREFSFETLQLHAGQEVDSASKSRAVPIYQTTSYVFEDAQEAE
                     DLFALRKSGNIYTRITNPTVSTFENRVAALEGGIGALATSSGMAAITYTILALAHAGD
                     HIVAATTLYGGTFNLFKETLPRYGITTSFVDIDDFEAVEAAIKDNTKLVFIETLGNPV
                     INIPDLEKLAEIAHNHHIPLVSDNTFATPYLINVFSHGVDISVHSATKFIGGHGTTIG
                     GVIVDSGKFDWAASGKFPQFVEEDPSYHNISYTRDIGAAAFIVAVRVQLLRDTGAALS
                     PFNAFLLLQGLETLSLRVERHVSNAEKIVDFLLSHPQVESVNYPSLPDSPYKALADKY
                     LPKGVGSIFSFQVKGGAEEARKVIDSLEIFSNLANVSDAKSLVVHPATTTHAQLAPED
                     LLAAGVTPNQIRLSVGLENINDLIEDLQLALDKL"
BASE COUNT          855 a          461 c          516 g          951 t
ORIGIN      
        1 gattttgtag gaaagaattt tgttgatgaa ttaccagatg atgactctaa aataatgatt
       61 cattttcata ctatgatact tgaaattgga tcaataattg ctgcacttaa aataataaaa
      121 attgtaaaca atgagtggca tgatagagta gtgaagtcat ctgttagata tgatataata
      181 cgaaatgtga catacgaatc tttgttttat agagttgtct tcggtataac taaaattttt
      241 gatatcagag aaaaaaatgg tatttttaag attctatcta agttgagaca tagtaccaaa
      301 gatagctctc ttttatcaat tttgaatact attcaagatg gtattgataa agaacaaaaa
      361 aatattgatg agattaaatt gcttcgcgac aaactcttgg ctcacttaga taaagagatg
      421 gtcttttcaa cagaaagatt agacattggc attctatact attattttga ggcgatagaa
      481 attaagtcca tatatacagc ttgtatcgag ttatataata ctttatatgg agataatcag
      541 caacaagtcg aacttcctaa aagagaaata attttgaaaa gattttttct tgaagaataa
      601 ctcgtattga ttagtttttt gtcttattat accgataaat ttataaaata cttgacctat
      661 cattttgaat ttgattaata aattatccat ctataagata aagtaggtca aattagtttg
      721 acctactgat gactagtcta ttttatggtg ttaattggtg tcgattttat ccaaatcaga
      781 ggatttcagt ccattttcat ggaagtttca ttttcgtaaa tgttgatata acgggatttg
      841 taaggggtga gaattcttcg ttttcaatag cgaaattctc taaaaatcag ggaggttatt
      901 cgatgttaga aataaaacga ttctcctttc tagaaaatcg gtaactgcaa tgttgacaac
      961 gataataagt ccgatcagct tcatagagca ttgcaaacat agctccctct atgccattga
     1021 ttgcatgacc aatcataccg gctttacgag aatatctttt ttgtgttgga acgtaagttt
     1081 gcagttttat tgacttacat tttggacatt tgagcgtttg tttagtgtcc atattattag
     1141 gatggcatct gcgatagatg ctataagaga ttattagtac tatgaggata atcggatcga
     1201 tatattctat taaggtattc ataatcttat tgtaaatctt gtccttattt tattcaagga
     1261 aaatgaatat tattttttct aaaataactg atcctatggc tatagttgtt aactatacat
     1321 atctctaatg tttaagtatt agtcaagctg gtaaaaatga ggtagaatac agaaaaagac
     1381 caaaggagta cctttatgac tagagaattt tcatttgaaa cccttcaact ccatgctgga
     1441 caagaagttg attctgcttc aaaatcacgt gcagtaccca tctatcaaac cacttcctat
     1501 gtttttgaag atgctcaaga agcagaagat ttatttgcct tgcgtaaatc agggaatatc
     1561 tatactcgta tcactaatcc gacggtctct acttttgaaa accgtgtagc agctttagaa
     1621 ggtggtattg gtgccttggc cacttcttca ggaatggcag ccatcacata cactattcta
     1681 gcattagcgc atgctggtga tcacattgtg gcggcaacga cactctatgg tgggactttt
     1741 aacctattca aagaaaccct tcctcgctat ggtattacaa caagctttgt agatattgat
     1801 gactttgaag cggtagaagc tgcgattaaa gataatacaa aactggtatt tattgaaacc
     1861 ttgggaaatc ctgttatcaa tattcctgat cttgaaaaat tggcagaaat tgcacacaac
     1921 caccacatcc cgcttgtttc agataatacc ttcgcaactc catatttgat taatgtattt
     1981 tctcatggtg ttgatatctc agttcattct gcaaccaagt ttatcggcgg gcatggtacg
     2041 accattggcg gtgtgattgt cgatagcggc aagtttgatt gggcggcatc tggtaagttt
     2101 ccgcaatttg ttgaagaaga tccaagctat cataacatta gttatactcg tgatattggt
     2161 gctgcagcat ttattgtagc agttcgtgta caattgcttc gcgatacagg tgctgccctt
     2221 tctccattca acgccttctt gcttttgcaa gggttagaaa ccttgtctct ccgtgtagag
     2281 cgtcatgtat ctaatgctga aaaaattgtt gactttttac ttagtcatcc tcaggtagag
     2341 tcggtcaatt atccatcact tccagatagt ccttataagg ctttggcgga taagtacctg
     2401 cctaagggag ttggctctat tttctctttc caagtaaaag gcggagcaga agaagcccgt
     2461 aaggttattg atagtttgga aatcttttct aatctagcaa acgtttccga tgccaaatca
     2521 ctagtagttc atccagctac aaccacgcat gctcagttag ctccggaaga tttacttgct
     2581 gctggggtca caccaaatca aatccgccta tctgttggtc ttgaaaatat caatgatttg
     2641 attgaagatt tgcaacttgc tcttgataaa ttataggttg tgaatcgaat agcatgactt
     2701 cattctttat gaataaataa aaggaaattt ggcgtgtgtg ccattttcct tttttatatc
     2761 tgttctagtt cttccagtcc att
//
LOCUS       DBNFEG010000201         2681 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.202, whole genome shotgun sequence.
ACCESSION   DBNFEG010000201 DBNFEG010000000
VERSION     DBNFEG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.202"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..741)
                     /gene="recX"
                     /locus_tag="AC1PNG_001966"
     CDS             complement(<1..741)
                     /gene="recX"
                     /locus_tag="AC1PNG_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HIP6702431.1"
                     /translation="MRITKIEKKKRLYLLEVDGQDSLYITEDTIVRFMLSKGKEITEQ
                     EFRELRDFAQFSYGKNLALYYLSFKQRTKKEVSDYLKKYEIEENNIVKIVMVLEEEKW
                     LDDGNYVDSYVRQNALNGDKGPAMIRQKLMQKGIPKPLIDKRLAEEDFSELAGKIGEK
                     LVGRYQRKLPLRALQDKVVQGLMGKGFSYETAKQSLSQLELVADEENEDDLIAKELDK
                     QYRKYSRKYEGYELKQRLIQALARKGYDF"
     gene            779..2131
                     /gene="rlmD"
                     /locus_tag="AC1PNG_001967"
     CDS             779..2131
                     /gene="rlmD"
                     /locus_tag="AC1PNG_001967"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIP6702432.1"
                     /translation="MNLQVNQRIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEEIFC
                     QITSVKRNFVEAKLLKINKESKFRVKPACDIYQACGGCQIMHLRYDKQLDFKQDLLQQ
                     ALKKFKPAGYENYTIYPTIGMDKPSHYRAKLQFQTRSFKDQVKAGLYAENSHRLIGIT
                     NCYVQDAETQEIINGIAKLLTKHRIPIYNERKQHGIRTVMVRKARKTGQVQVIFITSC
                     QVNLIKLIDELTSQFPSIITIALNWNRQKTSDIYGEKTEILWGEQAIDEAVLDYEFSL
                     SPRAFYQLNPQQTEILYGEAVKALDISGDEDLIDAYCGVGTIGFAFAKKVKSVRGMDI
                     IPEAIEDAKANAKRMGLSNTHYEAGKAEEIIPKWYQEGYRADALVVDPPRTGLDEKLL
                     KTILDYKPAKMVYVSCNVSTLARDLVELSKAYKVEYIQSVDMFPHTARTEAVVKLSKK
                     "
     gene            2415..2618
                     /locus_tag="AC1PNG_001968"
                     /pseudo
     CDS             2415..2618
                     /locus_tag="AC1PNG_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          879 a          597 c          444 g          761 t
ORIGIN      
        1 aaagtcataa ccctttcgag ctaaagcctg tatcaacctt tgcttcaatt cgtagccttc
       61 atatttacga ctatattttc tatactgctt atccaattcc ttggcaatta agtcatcttc
      121 attttcctca tcagccacaa gttccaactg actcaggctt tgtttggccg tctcataaga
      181 aaaacccttc cccatcaatc cctgtacaac tttatcttgc aaagctcgaa gaggcaattt
      241 tctttggtat ctacccacca atttctcccc aatcttccca gcaagctctg aaaaatcttc
      301 ctctgccagc cttttatcaa tcagcggttt aggaattcct ttctgcatca acttctgacg
      361 aatcatagct ggtcccttat ctccatttag agcattctga cggacatagc tatcaacata
      421 attcccatcg tccagccatt tttcctcttc taataccatc acaatcttga caatattgtt
      481 ttcttcaatt tcatattttt ttagataatc actaacttct tttttggtac gttgcttgaa
      541 ggaaaggtaa taaagtgcta gatttttccc ataagaaaac tgagcaaaat cgcgaagctc
      601 acgaaactcc tgctccgtga tttcctttcc cttactaagc atgaagcgaa caattgtgtc
      661 ttccgtaata tataaactat cctgaccatc cacttcaagt aaataaagac gtttcttttt
      721 ttcaattttt gtaattctca ttctttcatt atacaggatt tttcggtaaa ataataatat
      781 gaatctacaa gtaaaccaac gaatcccctt aaaaatcaaa cgaatgggca tcaacggaga
      841 aggcatcgga ttttatcaaa aaacgctagt ctttgtccct ggtgccctta agggagaaga
      901 aattttttgt caaattacct cagtcaaacg aaatttcgtt gaagcaaaat tgttaaaaat
      961 caacaaggaa tcaaaattcc gtgtcaagcc ggcctgtgac atctatcaag cctgtggtgg
     1021 ttgtcaaatc atgcacctcc gctatgataa acaattagac ttcaaacaag atctcttgca
     1081 gcaggcccta aaaaagttca aaccagctgg ctacgaaaac tacactattt atccaacaat
     1141 cggtatggat aagcctagcc actatcgtgc caaattacag ttccagactc gatctttcaa
     1201 agatcaagtc aaggccggtc tctatgcgga aaattcccat cgcttgattg gaattacgaa
     1261 ttgttacgtc caagatgccg aaacacaaga gattatcaac ggaatcgcaa agctactcac
     1321 caagcaccgt attcctatct ataatgagcg caagcaacac ggaatccgca cggttatggt
     1381 ccgcaaggct cgaaaaacag gtcaagttca agtgatcttt atcacctctt gtcaggttaa
     1441 tcttattaaa ttaattgatg aactcaccag tcaatttcca agtataatca ctattgctct
     1501 aaactggaat agacagaaaa cgagtgatat ttatggtgaa aaaacagaaa ttttatgggg
     1561 agaacaagct atcgatgaag ctgttttaga ttacgaattc tcactatctc cacgggcatt
     1621 ttaccaatta aatccccaac aaacagaaat tctctatggc gaggccgtca aggctttgga
     1681 tatttcagga gatgaagact tgattgatgc ctactgtggc gtgggcacaa tcggcttcgc
     1741 ctttgctaaa aaggtcaaat ctgttcgagg tatggacatt attccagaag ccattgagga
     1801 tgctaaagca aacgctaagc gaatgggact atccaatacc cactatgagg caggtaaagc
     1861 agaagaaatc attccaaagt ggtaccaaga aggctacagg gccgatgcct tggtagtcga
     1921 tcctccacga actggactcg atgaaaagct cctcaaaacc attttggact acaaaccagc
     1981 taaaatggtt tatgtatcct gcaacgtctc cactcttgca agggaccttg ttgagctaag
     2041 caaagcctac aaggtagaat acattcaatc tgtcgacatg tttcctcaca cagcacggac
     2101 cgaggcggtt gttaaactaa gcaaaaaata aaaggcctat aagactatga cgggtaaacc
     2161 cactatggtc atatagagcc tttttgagtg tagaaaaaag tcccctacga cctacactga
     2221 tacccattta aaatcaaaaa tagccaccta ccactcaaag tagatatgga gcattttgat
     2281 tttgttattt tttgagaaaa aaacagctac ttgaaatcca caatcctcaa ctcaaactta
     2341 taaattaagg ttttacatgt tataagtaaa ttagacttgc tatttttgat ttttattgag
     2401 tatgaaaaac aacaaaactc acaatttcca acaccaaatc agaaacaact aacaatctca
     2461 tcaaagtcat taaaagaaat gcctttggat tcaggaaaat gaaaactaga aaaacggatt
     2521 ttgatgcttt gaacagtgaa aagctgagga gagctctttt cagctcatct cctaaaaaca
     2581 agaaagagag aacgaatttc gtcctctcta ggtgttagct tttcatctac ccactacagt
     2641 tgacaaagag ccaaaaaaaa agaacattgc tactctctta c
//
LOCUS       DBNFEG010000202         2643 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.203, whole genome shotgun sequence.
ACCESSION   DBNFEG010000202 DBNFEG010000000
VERSION     DBNFEG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.203"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..1232)
                     /locus_tag="AC1PNG_001969"
     CDS             complement(<1..1232)
                     /locus_tag="AC1PNG_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HIP6702433.1"
                     /translation="MKLTIFDKVQNKTSQFAIDELTYYLKAIFDFELGRTTVEEDANI
                     LLSKEYFDNNDTIGIEMKNGNAILSGNSDISILIAVYRLLSEFGAVFTRPGRKHELIP
                     SLSISDWNEKELSIHETASYKHRGVCIEGADSYQNIKEFLDWLPKINMNSFFIQFENP
                     YSFLKRWYEHEFNPYADKEDFNTEIAQQMSDLLDEELSKRGLIHHRVGHGWTGEVLGY
                     SSKYGWESGLILPEEKKPLVAELNGKRELFNTAPILTSLCFSNPDVGERMCELIVNYA
                     KERKDVDYLHVWLSDARNNICECEKCKEEIPSDQYVRILNQLDRKLSEEGLNTKICFL
                     LYHELLFAPKHEVLENPERFTMMFAPITRTFEMSYADVDYENNVPLPNEYTRNNIVLP
                     NSLEENLSYLFSWQKVFNG"
     gene            complement(1237..2564)
                     /locus_tag="AC1PNG_001970"
                     /pseudo
     CDS             complement(1237..2564)
                     /locus_tag="AC1PNG_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
BASE COUNT          888 a          534 c          403 g          818 t
ORIGIN      
        1 ccattaaaca ctttttgcca actaaataaa tatgagagat tttcttcaag tgagttagga
       61 agaacaatat tatttcttgt atactcattt ggaagtggga cattgttttc atagtctaca
      121 tcagcataac tcatttcgaa agttctggta ataggcgcaa acatcattgt gaaacgttca
      181 ggattctcta aaacttcatg ctttggtgca aaaagcaatt catgataaag taaaaaacaa
      241 atctttgtat taagcccctc ttcacttaat ttccgatcta attgattcaa aattcgtaca
      301 tactgatctg agggtatttc ttctttacat ttttcacatt cacaaatatt gttccgtgca
      361 tcggataacc aaacatgtaa gtagtctaca tctttccttt cctttgcata gtttacaatt
      421 agttcacaca ttctttcacc aacatctgga tttgagaaac atagactagt tagtattggc
      481 gctgtattaa ataactcacg ttttccattt aattcagcga ctaggggttt cttttcttca
      541 ggtagtatta acccagattc ccatccgtac ttagatgaat atccaagtac ttcaccagtc
      601 caaccatgtc caacacgatg atgtattaaa cctcgttttg ataattcttc atctaaaagg
      661 tcgctcattt gttgtgcaat ttccgtattg aaatcttctt tatccgcata cggattaaat
      721 tcatgttcat accagcgctt taaaaatgaa tacggattct caaactgaat aaaaaagcta
      781 ttcatattaa tttttggtaa ccaatccaaa aattctttta tattctggta actatctgca
      841 ccttctatac atactcctcg atgtttataa ctggcagtct cgtgaataga gagctctttc
      901 tcattccagt cagatatcga taacgaggga attagttcat gttttctacc tggtctagtg
      961 aatacagctc caaattcact aagtaagcga tataccgcaa taagaataga gatatcagaa
     1021 ttacctgata atatcgcatt tccattcttc atctcaatcc caatcgtatc attattgtca
     1081 aaatattctt ttgagagtag aatatttgca tcttcctcaa cagttgtcct tcctaactca
     1141 aaatcaaaaa tggctttcaa gtaatatgta agttcatcta tagcaaattg agaagtttta
     1201 ttctgtactt tatcaaaaat cgttaatttc atatgatcac tctcctgatg cttcaagctc
     1261 taaattctta ttatcggcca ctttgaaaaa tggataataa ataatgaatg agactcctat
     1321 taaaataatt tgtaaaattg ctccttgaac actaccgcca gtagcaagga acccaccaag
     1381 aattggaggc attgtccaag gaagaacaac ccctgttgtc atcggaacta atccagttgc
     1441 catagcgaaa taggtaatta ttgcatttac tgttggcgtg agaataaaag gaatcatcat
     1501 aataggattc aaaacggttg gaaaactgaa cagaatagca gtattaatat taaagagaga
     1561 aggaataaag gagattcgtc ctaaactctt aaatgtacga cttttactaa agacaagtaa
     1621 gcaaatcgct aatcctactg tagcgccacc accgcccata aatacgaaca aatctttgaa
     1681 tggcaaagta ataatatgtg gtaatttttc tcccgcagct gctgcaactt gatttgcttc
     1741 tgtgaactgt aaccaaaccg gatcaacaaa agcatttaac acttgtccac cattaacgcc
     1801 agtaaaccag aaaagtgaat ttacaagcac aacaagcacc attccaccta gacttcctgc
     1861 aataagtcct agtggtttac ctacaattaa tcctaaaacc gctccaagtc caccttcaac
     1921 accggcagct tctaatccct ggaagacgca cgcccgaaaa tcaatgttgt taatgctgga
     1981 agcaatgcag caaacgactt acccacaacg tcagggacat tttttggcat tttaattgta
     2041 atacctcttt gaataaagaa gcgataaatt tcagctgaaa ctaatccaat agcaatagca
     2101 acaaataatg cacttgcgtt caaacttcca aatggtattg ctccagcaaa tacttgatta
     2161 atttgttcac cagattcgtt gaaaactatt gttgagaatc ttggtgcaag taaaataaaa
     2221 gaagataagg caataacacc tgcagaaggt ccatctgtct catatgattc ggataatcga
     2281 tatgcaatac cgaatgtagc tatgagagag atgatcgcca ttgttgattg atttaaccga
     2341 tttaaaattc ccaacaaacc aatttgctct aaaaagccta ctactgaaaa tcccccagaa
     2401 gcctgatccg gccaactgat tagcaacagg aagaacgagc caactaatac aagaggcata
     2461 gccatcataa aaccgtcacg aactgctttc aaatgacgtt gatttcctac cttagttcca
     2521 aaaggagcaa gtttatgttc aagaaaatgc attaatctat tcattttctt atcctccatt
     2581 aattttattt tgtaaaataa agatatcatg taagcgctta attgtcaagt ttttatttat
     2641 att
//
LOCUS       DBNFEG010000203         2640 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.204, whole genome shotgun sequence.
ACCESSION   DBNFEG010000203 DBNFEG010000000
VERSION     DBNFEG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.204"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            14..1306
                     /locus_tag="AC1PNG_001971"
     CDS             14..1306
                     /locus_tag="AC1PNG_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIP6702434.1"
                     /translation="MKELENYYGRLFTKYQLTAKEREIAEKVPSITKKNNCFRCGTTF
                     KEENKLPNDAYYCRACLLLGRVRSDEKLYHFPQKDFPITKCLKWKGQLTDWQQRISDG
                     LVANVENNRATLVHAVTGAGKTEMIYHTVASVIDKGGAVCLASPRIDVCIELYKRLQN
                     DFSVPISLLHGESEPYFRTPLVVATTHQLLKFYQAFDLVLIDEVDAFPYADNPMLYQA
                     ADNAVKEAGVQVFLTATSTDELDKKVRTGKLSRLSLPRRFHGNPLVVPQKVWFSKFDD
                     TLKKNRLVPKLKKAIEEQRKSGFPLLIFVPEISKGQEFTKIMKKTFPEETIGFVSSQT
                     ENRLEIVEGFRKREITVLISTTILERGVTFPCVDVFVVQANHYLYTASSLVQIAGRVG
                     RSIERPTGLLQFYHEGSTGAIEKAIAEIKQMNKEAGYV"
     gene            1299..1964
                     /locus_tag="AC1PNG_001972"
     CDS             1299..1964
                     /locus_tag="AC1PNG_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIP6702435.1"
                     /translation="MSNCLLCGQAMKNKTRFSDLIFFSKEKSGICEECFSTFEEIAEQ
                     HCPHCYKNGESESCKDCQYWQNQGKPVVHTALYQYNQAMAHYFSRYKFQGDYVLRNIF
                     AKKLRIALSQFPDYTIVPIPISQKRLSERGFNQVEGLLDATNIPYQSLLGKYESQKQS
                     SKNRAERLEAKQMFYLLDEKEVPEKILLFDDIYTTGATIQLAVELFMKIGRKEIKTFS
                     LTR"
     gene            2041..2583
                     /gene="hpf"
                     /locus_tag="AC1PNG_001973"
     CDS             2041..2583
                     /gene="hpf"
                     /locus_tag="AC1PNG_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIP6702436.1"
                     /translation="MIKFSIRGENLEVTEALRTYVEEKVAKIEKYFNEEQELNAKVNL
                     KVYRDKRAKVEVTIPVGAVTLRAEDISQEMYGSIDLVVDKIERQIRRNKTKIERKHRQ
                     KVATGQVFTDELVEQTGEEVKVVRTKQVDLKPMDMEEAVLQLELLGHDFFIYTDANDG
                     TTNVLYRREDGDLGLLEVRQ"
BASE COUNT          941 a          456 c          537 g          706 t
ORIGIN      
        1 gaataaataa gcgatgaaag aattagaaaa ttattatgga agattattta ccaaatacca
       61 attgacagca aaagaaagag aaatagcaga aaaagtgcca agtattacaa aaaagaataa
      121 ctgctttcgc tgtggaacaa cttttaaaga agaaaacaaa ttgccaaacg atgcttatta
      181 ctgtcgagcc tgcttgcttc taggcagagt acggtcagac gaaaaactct atcattttcc
      241 tcagaaagat tttccaatca ctaagtgttt aaagtggaaa ggtcaactaa ctgattggca
      301 acaaagaatt tcagatggac tagttgcaaa cgtggaaaat aatcgtgcga cattggttca
      361 tgcagtaaca ggagcaggta agacagaaat gatctaccac accgttgcct cagtgattga
      421 taaaggcgga gcggtttgcc tagccagtcc tcgaattgat gtttgtatcg aactctataa
      481 acgtctgcaa aatgactttt cagttccaat tagtttacta catggagagt ctgaacccta
      541 tttccgaacc ccattagttg tagcaaccac acatcagtta ttaaaatttt atcaggcctt
      601 tgatttggtt ttgattgatg aagtagacgc ctttccctat gcagataatc ccatgctcta
      661 tcaagcagca gacaatgcgg tcaaggaagc cggtgttcaa gtttttctga cagcgacttc
      721 aacagatgaa ttggataaaa aagtcagaac aggtaaatta agtcgtctta gtttgccaag
      781 gcgctttcat ggcaacccac ttgttgtccc gcaaaaagtc tggtttagta aattcgatga
      841 taccctaaag aaaaatagac tagtcccaaa gttgaaaaaa gcgattgaag aacagagaaa
      901 gtcgggcttt cccttactca tttttgtccc agaaatctcc aaaggtcaag aatttaccaa
      961 gataatgaaa aaaacattcc cagaagaaac aattggcttt gtatccagtc aaacagaaaa
     1021 tcgccttgaa atagttgaag ggtttcgcaa gagagaaatc acagtcttaa tctcgactac
     1081 tattcttgaa cgtggggtga ccttcccatg tgtagacgtc tttgttgttc aagctaatca
     1141 ttacctctac acagcgtcaa gtcttgttca gattgcaggc cgggtcggaa ggagtataga
     1201 acgtccgact ggtttacttc agttttatca tgagggaagt acaggagcca ttgaaaaggc
     1261 aatcgctgaa attaaacaga tgaacaagga ggctggttat gtctaattgt ctattgtgcg
     1321 gtcaagctat gaaaaataaa acaagatttt cagatctcat cttctttagt aaagaaaaat
     1381 cgggcatctg tgaagaatgt ttttcgactt ttgaggaaat agcagagcaa cactgtccac
     1441 attgttataa aaatggagaa tcggaaagtt gcaaggattg tcagtactgg cagaatcaag
     1501 ggaaaccagt tgtccacaca gctttgtacc aatataatca agcgatggca cattatttta
     1561 gtcgctacaa atttcaagga gactatgttt taagaaatat ttttgctaaa aagcttagaa
     1621 tagcgttaag ccaatttcct gactacacaa ttgttcccat accaattagt caaaaacgac
     1681 tatcagaacg cggttttaat caagtggaag gacttttgga tgctacaaat attccttatc
     1741 agtcccttct tggaaagtat gaaagccaaa aacaatcatc taaaaatcgt gcagagagac
     1801 tagaagcaaa acagatgttc tatctattag atgaaaaaga agtgccagaa aaaatattat
     1861 tattcgacga tatatataca acaggagcca caatccaact tgctgtagaa cttttcatga
     1921 aaattggtag gaaagaaatc aaaacatttt cactaacacg ataagaaaac ggttgcaata
     1981 ttcctataat gtggtataat aatagtgaag aaaggcgaaa gccaagaaag aggtactaat
     2041 atgattaaat tcagtattcg tggagaaaac cttgaagtta cagaagcact tcgcacctat
     2101 gtagaagaga aagtggctaa gattgagaaa tatttcaatg aagaacaaga gttgaatgct
     2161 aaagtaaatc taaaagttta ccgtgacaag cgcgcaaaag tagaagtgac aattccagtt
     2221 ggagcagtta cacttcgcgc agaagatatt tcacaagaaa tgtatggctc tatcgatctt
     2281 gtagtagata agattgaacg ccaaattcgt cgtaacaaaa ccaagattga acgaaaacat
     2341 cgtcaaaaag tggcaactgg tcaagtcttt acagatgaac ttgttgagca aacaggtgag
     2401 gaagtaaaag tggttcgtac taagcaagta gacttgaaac caatggatat ggaagaagca
     2461 gtcctccaat tggagttgct cggacacgat ttctttatct atacagatgc taatgacggt
     2521 acaacaaatg tattgtatag acgcgaagat ggagatttgg gtcttctaga agtacgtcaa
     2581 taaagataat aaaacagttg cagtttttgc agctgtttta ttattttcaa aaaaccggtt
//
LOCUS       DBNFEG010000204         2632 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.205, whole genome shotgun sequence.
ACCESSION   DBNFEG010000204 DBNFEG010000000
VERSION     DBNFEG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.205"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            13..723
                     /locus_tag="AC1PNG_001974"
     CDS             13..723
                     /locus_tag="AC1PNG_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIP6702437.1"
                     /translation="MKVPKYQLIQNDLRQQIVSGKFENGDKFYTESELTKLFNVSSIT
                     VIRAVNELVKDGYLVRQQGKGTFVSRSRKGRLVEFSDIEIFPMDKDKVTVLSCEKGNK
                     PDILEKLNLDKNEFYYKIVRVRAAEDTPYIFHNSYIPQRYIQNPDAPLEHYQSIYQRF
                     KLDYNIHMSEEPFVETNEIVSPCPKEVATHLKLKATEPAVLQNKTTTNSTSGEVMEYT
                     ETYKHWKYYKFEITANHR"
     gene            complement(863..1594)
                     /locus_tag="AC1PNG_001975"
     CDS             complement(863..1594)
                     /locus_tag="AC1PNG_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIP6702438.1"
                     /translation="MVSQTVEKPLYLQLVDELEVAIRERMAPNDKLFSERELTQVYGV
                     SRITVRLALQELEKRGLVYKKHGKGTYVSEISDTAVDLSQAYSFTEQMKKIGKVPRTS
                     ILSFELVKASDYIAQHLQLSQGEEVFEVERLRLADEVPMMLERTYVPASLFPGLAAQR
                     MKSTPLYEIFSEDYHQVIRLAEEEFYASIALDNEAKILGIPSNSPVLHLVRKTYNDKN
                     RIIEFTFSIARADQFRYKITHQRGY"
     gene            complement(1694..2011)
                     /locus_tag="AC1PNG_001976"
     CDS             complement(1694..2011)
                     /locus_tag="AC1PNG_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HIP6702439.1"
                     /translation="MAFEQTNGRYASFGVVTSLPGEVIDSFWYVIDHYLKGVIPLKSV
                     IRFSIKNRRGKITLVFSQEGYKNVLAVDLSSRFDPFYPSTILVMDKQGKETITLPDEV
                     TLL"
     gene            complement(2069..2602)
                     /gene="ntdP"
                     /locus_tag="AC1PNG_001977"
     CDS             complement(2069..2602)
                     /gene="ntdP"
                     /locus_tag="AC1PNG_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017110 - nucleoside diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside tri-diphosphate phosphatase"
                     /protein_id="HIP6702440.1"
                     /translation="MKLPKEGDFITIQSYKHDGEIHRTWRDTMVLKTTENAIIGVNNH
                     TLVTENDGRRWVTREPAIVYFHKKYWFNIIAMIRENGVSYYCNLASPYVLDNEALKYI
                     DYDLDVKVFADGEKRLLDVDEYERHRKAMKYSDDIDFILKENVKILVDWINNQRGPFS
                     PAYVNIWYKRYLELRSR"
BASE COUNT          873 a          594 c          403 g          762 t
ORIGIN      
        1 aaggaaataa gtatgaaagt accgaagtac caacttatac aaaacgactt acgccaacag
       61 attgtttctg gcaaatttga aaatggcgac aaattttaca cagaatctga attaacaaaa
      121 ctatttaatg ttagctccat taccgttatc cgtgcagtaa acgaactagt taaagatggt
      181 tacctcgttc gccaacaagg taaaggaacc tttgtatctc gttcacgcaa aggacgtctt
      241 gtagaatttt ctgatattga gatttttcct atggataagg ataaggttac tgttctctcg
      301 tgcgaaaaag gcaataaacc cgatatcctt gaaaaactaa atcttgacaa gaatgagttt
      361 tactataaaa ttgtgcgtgt ccgcgcagca gaagacactc catacatctt ccacaattca
      421 tacatccctc aacgatatat ccagaatccc gatgctccac tcgagcacta tcaatcaatc
      481 tatcaacgct tcaaactgga ctacaatatc cacatgtccg aggaaccatt tgtagaaacg
      541 aatgaaattg tcagcccatg tccaaaagag gttgccaccc atttgaaatt aaaagcgact
      601 gaaccagctg tactacaaaa caaaacaact actaacagta ctagcggtga agtgatggaa
      661 tatactgaaa cttacaaaca ctggaagtat tacaaatttg aaattacagc caaccatcgc
      721 taaaaaaacg cagactaacg tccgcgtttt tttcataccc aataacaatc aaaagtagcc
      781 taggaaacga aggcgaagat agaactggat ttcatcaagg ctcttgacaa cgaatagttt
      841 tgattttcga agagtattga ttctaatatc cccgttgatg ggtaatcttg taacgaaact
      901 ggtccgctct agcaatacta aacgtaaact cgataattct attcttatca ttatacgttt
      961 ttcttaccaa atgaagcact ggactattgc ttggaattcc gagtatctta gcttcattat
     1021 ccaaagcaat acttgcatag aactcttctt ctgccaaacg aatcacctga tgataatcct
     1081 ctgaaaaaat ttcatacaag ggagttgact tcattcgctg agcagctaat ccaggaaata
     1141 gcgaggcagg aacatatgtc ctttccaaca tcatcggaac ctcatctgca agtctcaaac
     1201 gctcaacttc aaacacttcc tcaccctgac taagttgtag atgctgcgca atatagtccg
     1261 acgccttaac caattcaaag gataaaatag atgtcctcgg tactttccca atttttttca
     1321 tttgctcagt aaaactatag gcctgagaca aatcaactgc agtatctgaa atctcagata
     1381 cataggttcc cttaccatgc ttcttataga ctaagccacg tttttccaat tcttgcaaag
     1441 ccaaacgaac tgtaatacga ctgacaccat aaacctgtgt caactcacgc tctgaaaata
     1501 acttgtcatt cggagccatc ctctcacgaa ttgcaacttc caattcatca accaactgca
     1561 aatagagagg tttttcaaca gtttgactaa ccatagactc ctcctttcta acccacataa
     1621 gcttacaaat ctattttaca ccaaatttca tatattaaca atatcttagg tgataaaata
     1681 ccagctcttc aagctaaagc aaggtcacct catctggcaa agtaatagtc tcttttccct
     1741 gcttgtccat taccaagatt gttgagggat aaaaaggatc aaaacgacta cttagatcta
     1801 cagccagtac atttttataa ccttcctgcg aaaagaccag cgtaatctta ccacgacgat
     1861 ttttgataga aaaacggatt acacttttta atggaataac cccttttaag tagtgatcaa
     1921 taacatacca gaaactatca atcacttccc caggaagcga ggtcacaact ccaaaacttg
     1981 catagcgccc atttgtttgt tcaaaggcca taagcccctc ctttacaata agaaaaaagc
     2041 ctgtaaaaca ggattttctt ttctaacttt atctacttct cagttctaaa tagcgtttgt
     2101 accagatatt aacgtaagca ggagagaatg gtccgcgctg gttattaatc caatcaacca
     2161 gtatcttgac attttccttc aaaataaaat caatatcatc cgaatacttc atcgccttac
     2221 gatgacgctc atattcatcc acatccagca gtctcttttc accatcagca aagaccttaa
     2281 catctaggtc ataatcaatg tatttgagtg cctcattgtc gagaacatat ggactagcaa
     2341 ggttacaata ataggaaact ccgttttcac gaatcattgc aatgatattg aaccagtatt
     2401 ttttatgaaa ataaacgatt gctggttctc ttgttaccca acgcctacca tcattttcag
     2461 tcactaaggt atgattattg actccaataa tagcattttc cgttgttttt aataccatgg
     2521 tgtcacgcca agtgcgatga atttcaccgt catgcttata actttgaatt gtaataaagt
     2581 cgccttcctt tggtaatttc actttgttac caactttcta caattacaat tt
//
LOCUS       DBNFEG010000205         2578 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.206, whole genome shotgun sequence.
ACCESSION   DBNFEG010000205 DBNFEG010000000
VERSION     DBNFEG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.206"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..518
                     /locus_tag="AC1PNG_001978"
     CDS             <1..518
                     /locus_tag="AC1PNG_001978"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIP6702441.1"
                     /translation="HENKLQKNVKFLGNLDNPWEEVTDRDICVFPSSMETFGLVYVEA
                     ILRGLPVILSDNLGHLTAFEIFSAGQIYKLGDIEELVTKLEQVIESFRNFSDRARLDV
                     ERLRTLYTPEVAYKEILNSINHLEYTGHQNTNLSHIKELLTLNMHPTRLERYARLISN
                     MATKIRHKLRL"
     gene            622..1338
                     /locus_tag="AC1PNG_001979"
     CDS             622..1338
                     /locus_tag="AC1PNG_001979"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIP6702442.1"
                     /translation="MKVLMIIPAYNEEESILQTVQGIIDYRNSVNFQLDYVVINDGST
                     DSTKEILIQNKLNAVHLVQNLGIGGAVQTGYKYALDNDYDVAVQFDGDGQHDVRSLNS
                     LIQPILVGQADMVIGSRFVGETLSEFQTSFMRRFGIGVISNMIKLTTGNRIWDTTSGY
                     RLGNRKVIAQFAKRYPIKYPEPESTVHLLKQNFQVVEAPANMFERAGGVSSITPFKSI
                     RYMVEVCSSILIASLMKEGE"
     gene            1338..1694
                     /locus_tag="AC1PNG_001980"
     CDS             1338..1694
                     /locus_tag="AC1PNG_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HIP6702443.1"
                     /translation="MTIQALAMFLASLGFLYFIFRNINKNKILFEHAFMWIVIGFGLI
                     VFALFDVIPIKLAYLFGFGLTSNFLLSVAIFVLLVIGFLHSMALSQQKQQIKNLIQEV
                     SMAKKRISELEEHHAE"
     gene            1684..2454
                     /locus_tag="AC1PNG_001981"
     CDS             1684..2454
                     /locus_tag="AC1PNG_001981"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIP6702444.1"
                     /translation="MQNNHTFVICAYGDSKYLEECIHSLKQQTLQSQLILYTSTPSEY
                     IEQICQKNAVVMHTAQGGGIGKDWNLALSFVTTPYATIAHQDDIYLPDYCEKIMSQMT
                     EETLIAYSDYQEVKEGVAISLTSNLKIKQLMLRTMAMFPKWKFWRNRVLAFGNPISCP
                     AVTYNLKKLNDFKFNEEMKVSLDWFAWYQIAQKNGSFTFVDESLMYHRIHEESETTNS
                     IENNIRTKEDYEMYLLFWPKFIAKFLLSYYVKSQETNN"
BASE COUNT          865 a          381 c          496 g          836 t
ORIGIN      
        1 tccatgaaaa taaattacag aaaaatgtta aatttttagg taacttagat aatccttggg
       61 aagaagtgac tgatagagat atctgtgttt tcccctcatc tatggaaact tttggcttag
      121 tctatgttga agcaatctta agagggcttc cagtaattct atcagataat ttaggacatt
      181 tgacggcatt tgaaatattc tcagcaggtc aaatttataa attgggcgat atagaagagt
      241 tggtgactaa gttagagcaa gtgattgaat cctttagaaa tttttcagat agggctcggc
      301 ttgatgtgga acgtttacgt actctatata caccggaagt tgcctataaa gaaatactga
      361 acagcatcaa tcacttagaa tatactggac accaaaatac aaatttaagt cacataaaag
      421 agttactgac tttaaatatg catccaacaa gattggagcg ttatgcaaga ttgatcagca
      481 atatggcgac aaaaatcaga cataaacttc gactttaatg aagaatagga tagctatgct
      541 aaaagatgta tattatagtt tcttcaaatg agtaagtaga atctaaaaag ttatatagaa
      601 ttctagtttt agagagaaaa aatgaaagta ttaatgatta tccctgccta caatgaagaa
      661 gaaagtatcc ttcaaactgt tcagggaatt atagattata gaaatagtgt gaatttccaa
      721 ttggattatg ttgtcattaa tgatggttca acagatagca cgaaagaaat tttgattcaa
      781 aataaattaa atgctgttca cctagtacaa aatttaggca ttggtggagc tgttcagacg
      841 ggatataaat atgccttgga caatgactat gatgtagcag ttcaatttga tggtgatggt
      901 cagcatgacg ttcgttcatt aaatagctta attcagccaa tcttagttgg tcaggctgat
      961 atggtgattg gctcacgttt tgttggtgag accttgtcag aatttcagac tagttttatg
     1021 agacgatttg ggataggtgt tatttcaaat atgattaaat taacaactgg aaaccgaatt
     1081 tgggatacta catcaggtta tcgtttggga aatagaaaag tgattgcaca atttgcaaaa
     1141 cgatacccaa ttaaatatcc ggaaccggaa tcaactgttc atttactaaa gcaaaatttc
     1201 caagtagtag aagcaccggc caatatgttt gaaagagcgg gaggggtttc ttcgattact
     1261 ccatttaagt ccatccgcta catggtagaa gtttgctcat ctattttgat tgcttcgctt
     1321 atgaaggagg gagaataatg accatacaag cattagctat gtttcttgct tcactaggtt
     1381 ttttgtattt tatttttagg aatattaata aaaataagat cttattcgaa catgccttta
     1441 tgtggattgt tattggcttc ggactgattg tttttgcgct ctttgatgtt attcctatta
     1501 aattggctta cttattcggg tttggtctga cttccaattt cttgttatct gtggcaattt
     1561 ttgttctctt ggtgattgga tttttgcata gtatggcttt atctcaacaa aagcagcaga
     1621 ttaaaaattt aattcaagaa gtatcaatgg ctaaaaaaag aatctctgag ttggaggagc
     1681 atcatgcaga ataaccatac atttgttatc tgtgcttatg gagatagtaa gtatctagaa
     1741 gaatgcatcc attcgttaaa gcagcaaaca cttcaatcac aactcatcct atacactagt
     1801 actccgagtg aatatataga acaaatttgc caaaagaatg cagtcgtaat gcataccgca
     1861 caaggaggag gtattggaaa agattggaat ttagcactct cttttgtaac aaccccttat
     1921 gctacaattg ctcatcaaga tgatatttat ttaccagact attgtgaaaa aataatgtct
     1981 cagatgacag aggaaacgtt gattgcttat tcagattacc aggaagttaa ggaaggtgtg
     2041 gctatttcct taacttcaaa tttgaaaatc aagcaactca tgttacggac aatggccatg
     2101 tttcccaaat ggaagttttg gcggaatagg gttttagcct ttggtaatcc aatttcttgt
     2161 ccagctgtga cctataattt gaagaaactg aatgatttca agtttaatga agaaatgaaa
     2221 gtttctcttg attggtttgc ttggtatcag attgcacaaa aaaatgggag ctttaccttt
     2281 gtagatgaga gtttgatgta tcatcgtatc catgaagaat cggagacgac aaatagcatt
     2341 gaaaacaata ttcgtacaaa agaagattat gaaatgtatt tgttattctg gccaaagttt
     2401 attgccaagt ttctcttgag ttactatgtt aagagtcaag agacgaataa ctgaaatatt
     2461 ttagctatcg atcattgcgt tcccaccaat tcctactcaa aatatttcaa aagcatttca
     2521 gaagttttac aaactaacta atattgtcat gtaaatgtaa taaaaaattt caaaagat
//
LOCUS       DBNFEG010000206         2563 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.207, whole genome shotgun sequence.
ACCESSION   DBNFEG010000206 DBNFEG010000000
VERSION     DBNFEG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.207"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            178..1767
                     /locus_tag="AC1PNG_001982"
     CDS             178..1767
                     /locus_tag="AC1PNG_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIP6702445.1"
                     /translation="MSKQKVVTNLLLSTAVLGGFLICHVSSVSAETGNLQEIPTESAT
                     VSSENQTATAVDGISPTDNQLPTSEEGDEQAPEEKLVSSDSTREESATQVATDPDAPS
                     QSLSVSGAEISTADSNIQQAEEKLIQESNYHRFDTHQGEQIRKAVSIEKISADNDEIK
                     WKVTFDRKNWTFSGEGSGYYFILLKGLQLTKIIDSQSEEDIFNKFPKDVNSEANSGGQ
                     PYRFFSREKNANNDERSFESQWGWSVGRVGGELEKWKEESSSVYFLQVQRDSVAKEKA
                     TYELTAQVTNREMQTFPVLAVVKNFKERIFFSRDEITSAAGLRLKVSESAKPNNEGIT
                     IIGDSSPESDRTPEPRGDRTSDIVVPKADVPKVEQPKPKDPEVQLPAPKAPESVPPKV
                     MPPAPKVPDVKTPKEAPLPPKTPEVEVPKVMPPTPKVPDVKTPKVEYPDTDAPVIESP
                     KAPETERPEVLPPTPKVPEVEVPKVIPPTSKVPEAELPKVEQPKPKVPEVHLPAPKSP
                     ETEPPKVMSPAPKVPDVKTPK"
     gene            2041..2187
                     /locus_tag="AC1PNG_001983"
     CDS             2041..2187
                     /locus_tag="AC1PNG_001983"
                     /inference="COORDINATES: protein motif:HMM:NF012948.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIP6702446.1"
                     /translation="MAPKVGIQNETLPNTGETSNTLVWLGGALATLASSLYLFKQHRQ
                     REEE"
BASE COUNT          879 a          497 c          565 g          622 t
ORIGIN      
        1 aaagagcttt tatgtaagag atagtttttc gctaatcaag ttaatttttt gagggaatgc
       61 tggctgtatt ttgaaaaaaa cgaacgatga gtagcttaaa aatgccttag atgggagtgc
      121 taacctgtaa atacaggttc ttagtaaatt gaagtaaata gagtaaagga gcacattatg
      181 agcaaacaaa aagttgtgac aaatctattg cttagtacag ctgtactagg tggttttcta
      241 atatgtcacg tatcatctgt aagcgcagaa acaggcaatc ttcaagaaat acctactgaa
      301 tctgctacgg ttagctcaga aaatcaaact gcgaccgcag tcgatggaat ttcacctacg
      361 gataaccaat tacctacgtc agaagaaggt gatgaacagg ctccagaaga aaaactagtt
      421 tctagcgatt ctacgagaga agaaagtgct acgcaggtag cgacggatcc ggatgcgcca
      481 tctcaatcat taagtgtgtc aggggcggaa atttcaacgg cagattcaaa tattcaacaa
      541 gccgaagaga aactgattca agaatcgaat tatcatagat ttgacactca tcaaggtgaa
      601 cagattagga aggcagtatc tatagaaaaa atatcggcag ataatgacga aattaagtgg
      661 aaagtaactt ttgatagaaa aaattggacc tttagtggag aggggagtgg ctattacttt
      721 attcttctaa aaggcttaca attaaccaaa attattgata gccagtctga agaggatatt
      781 ttcaataagt tccctaagga tgtcaatagt gaggcaaatt ctggcgggca gccgtatcgc
      841 ttttttagta gagaaaaaaa tgctaataat gatgagcgta gttttgaaag tcagtggggc
      901 tggtctgttg gacgtgtagg aggagagcta gaaaaatgga aggaagaaag ttccagcgtc
      961 tattttctac aagtacagcg agattccgtt gctaaagaaa aagcgacata tgaactgacg
     1021 gcacaggtga caaatcgaga aatgcagact ttcccggttt tagctgtggt gaagaatttt
     1081 aaagagcgaa tatttttctc cagagatgag attacttctg ctgctggttt gagattaaag
     1141 gtatcggaaa gtgctaagcc aaataatgaa gggattacta ttatcggaga ttcatctcca
     1201 gaaagcgaca ggactcctga accgcgggga gataggactt cggatatagt tgtaccaaag
     1261 gcagatgttc caaaagttga gcaaccaaaa ccaaaggacc ctgaagttca actgcctgct
     1321 cctaaggctc cggaatctgt gccaccaaaa gtaatgcctc cagcacccaa agttccagac
     1381 gttaagacgc ccaaagaggc gcctctgcca ccaaaaactc cggaagttga ggttccaaaa
     1441 gtaatgcctc cgacaccgaa ggttccagat gttaagacgc caaaagttga gtaccctgat
     1501 acagatgctc ctgtgattga gtcaccaaaa gctcctgaaa ctgaacgacc agaagtctta
     1561 ccaccgacgc caaaagtccc agaagttgag gttccgaaag taattccacc gacctcaaaa
     1621 gttccagaag ctgagctacc gaaagttgag cagccaaaac cgaaggttcc agaagttcac
     1681 cttcctgctc ctaagtctcc ggaaactgag ccacccaaag taatgtcccc agcacccaaa
     1741 gttccagacg ttaagacgcc aaaataggcg cctctgccac caaaaactcc gggcgttgag
     1801 gttccgaaag taattccacc gaccccaaaa gccccagaag ttgaggttcc gaaagtaaca
     1861 ccaccaacct caaaaattcc agacgttaag acgccaaaat ctccggaagt tgcgcaccca
     1921 gaagttgaga aacccgccgg aatccaacca gaaaagggtc aacctgaggt taataaaccc
     1981 aatattattc attcggaggt gcttcaatcc gaagttaaga aaacggaatc tggagtttca
     2041 atggctccaa aggtaggaat acaaaatgag actttaccaa atacgggtga aacgagcaac
     2101 accctagtat ggctaggcgg tgctctagct acccttgcat caagtttgta tcttttcaaa
     2161 caacacaggc aaagagaaga ggaatgattt cacaccaata ttatggtttt tggaacgcca
     2221 ataaggctta tctaccatgg actaagaaaa tttagaacag ttgtaaatag aaaacgttcc
     2281 aaaatctgaa ctgcacccca aaagttagag gttaactcta atttttgagg tgcttttctt
     2341 gtaaggtcaa gacacaaaga caagattgaa atttatcgct tgcaatgata tgaaggagtt
     2401 ggttctatat tttgttttat caatactagt ttgaatgtta ttcgagaatt tgtaggacat
     2461 tctgaggatg gtaaggagat agtgaaaatc tatcttcatg taatacaaaa aagaaaacat
     2521 aagattgaac aggctatgct agatttagca gaaataatta ttt
//
LOCUS       DBNFEG010000207         2429 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.208, whole genome shotgun sequence.
ACCESSION   DBNFEG010000207 DBNFEG010000000
VERSION     DBNFEG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.208"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(102..440)
                     /locus_tag="AC1PNG_001984"
     CDS             complement(102..440)
                     /locus_tag="AC1PNG_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HIP6702447.1"
                     /translation="MQVIKRSGEIVEFDPDKIYQAIIKAAQTVYVIDETWRKNLAQVT
                     KKVVLDLEEAHTERPTISMVQSQVEHRLLDAGYISIAEHYISYRLQRDLERNGYGDKI
                     IVHLRFEQVR"
     gene            607..2397
                     /gene="pepF"
                     /locus_tag="AC1PNG_001985"
     CDS             607..2397
                     /gene="pepF"
                     /locus_tag="AC1PNG_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HIP6702448.1"
                     /translation="MTNAMPKRQEIDVQLTWDTDILFPTPDNYKENLAAYAKQVTAFE
                     SNYKGKLTDKDTIVSALTEYEKIVILDSRLSHYAFLPLEVDKMNTELASLANEYDLVS
                     AKARPQRDFLYSELGQLDEAFLLELKDEQPQWAAFFDAILRDKPHQLHPLQEELLSNF
                     APTFGQPYNNYGVTKFEDMTFENFEANGQTLDNSYVLFENDYELSHDTEIRRNSAAGF
                     YSTLKKYKNTTAATYLSHIKNEQIEARLRGFDNTIDFLLHSQNVSRDLFDRQIDVIMK
                     ELAPHMRRYVKLVAKAHGLDKITHADLKISLPSEYNQRITPEESKQFLIDCLGILGED
                     YVKMIEQSFDERWIDFAQNEGKATGGFCATLYDGPSYILLSWTGLMNEVLVLAHELGH
                     AGHFQLAKKQSVLSYDPSLYFIEAPSTANEVLTCNTLLKNNQDPGFQAYLISELISRT
                     YFHNMVTHLLEAAFQREVYTRLDNEEYLNGDILCQIKLDVIKEFWGEDFEIGDDAGLI
                     WMRQPHYYIGLYPYTYSAGLTIGTAMAKQLEEHPEEVVEKWLETLSLGASLSAQDLAK
                     HAGVDVSTDQPLKETIAYVGSLVDKLETLI"
BASE COUNT          728 a          525 c          457 g          719 t
ORIGIN      
        1 tcagaatatc acattttttt caaaaagttt ttgaaatgct attttcttta aacaatgtaa
       61 agatatataa aagcaaaaac ctagatttct ctaggtttct cttaacgtac ttgttcaaaa
      121 cgcagatgta caataatctt atcgccatag ccgttacgtt ctaaatcacg ttgcaagcga
      181 taggaaatgt aatgctcagc aatagaaata taccctgcat ctaaaagacg atgttcaact
      241 tgcgactgaa ccatgctaat cgttggtcgc tctgtatgag cttcttccaa gtcaagcact
      301 actttctttg tgacctgagc aagatttttc cgccaagttt catcaatgac atagactgtc
      361 tgtgcagctt taatgatggc ttggtagatt ttatctggat caaattcaac aatctctcca
      421 ctacgtttaa tgacttgcat aaaagctcct ttctggtttt cagtaaaccc tttcatactt
      481 acattatcta ggtttttgtg tattttgtca agtattcttt ttcataaatt ttgccttggc
      541 ttattttcgc tgaaagccct ttagtatgat ataatggacc tattaaaatg aaataaggag
      601 aaaatcatga caaatgcaat gcctaaacgt caagaaattg atgtgcaatt aacttgggat
      661 acggacattc tatttccgac ccctgataac tacaaagaaa atttggcagc ctacgctaag
      721 caagtgacag ctttcgaatc gaattacaaa ggaaagctaa ctgataagga taccatcgtt
      781 tccgccttaa cagaatacga aaaaatcgtt attcttgata gcagactttc ccactatgcc
      841 ttccttcctt tggaagtcga taagatgaat actgaattgg cgagcctggc caacgaatac
      901 gacctcgttt ctgccaaggc acgtcctcaa cgagatttcc tctattcaga acttgggcaa
      961 ttagatgaag cctttctact tgaattaaaa gatgaacaac cacaatgggc tgctttcttc
     1021 gatgctattc tccgtgacaa gccgcatcag cttcatccac tacaagaaga acttttatca
     1081 aattttgctc caacatttgg tcagccctat aacaactacg gtgtgaccaa gtttgaagat
     1141 atgacatttg aaaacttcga ggcaaatggt caaacgctcg acaatagcta tgtgctcttt
     1201 gaaaatgatt atgagctcag ccacgatacc gaaatccgtc gcaattcagc tgctggtttc
     1261 tattcaacct tgaagaaata caagaataca actgcagcaa cttatttgtc tcatatcaag
     1321 aatgagcaaa tcgaagctcg cttgcgtgga tttgacaata cgattgactt cctcttgcat
     1381 agtcaaaatg tctctcgtga tctcttcgac cgtcagattg atgtcatcat gaaagaattg
     1441 gctcctcaca tgcgtcgcta tgtaaaattg gttgctaagg ctcatggatt ggacaagatc
     1501 acacatgctg acttgaaaat cagtctacca tctgaataca accaacgcat cacacctgaa
     1561 gaatccaaac aattcttgat tgactgcctg ggcattcttg gtgaagatta tgtgaaaatg
     1621 attgaacaat catttgatga gcgttggatt gactttgctc aaaacgaagg taaggctact
     1681 ggtggcttct gtgctactct ttacgatgga ccatcttaca tcctactttc atggacaggc
     1741 ttgatgaacg aggtcttggt attggctcac gaattgggac atgctggtca tttccaattg
     1801 gctaagaaac aaagtgtcct tagctacgat ccatccctct acttcatcga ggcgccttct
     1861 actgcaaatg aagtcttgac atgtaacacc cttttgaaaa acaaccaaga ccctggcttc
     1921 caggcttact tgattagcga gttgatcagc cgtacttact tccacaacat ggtcactcac
     1981 ttgcttgaag cagccttcca acgtgaagtc tacactcgtt tggacaatga agaatacctc
     2041 aacggtgata ttctctgcca aatcaagttg gatgtcatca aggaattctg gggtgaagac
     2101 tttgaaatcg gtgacgatgc aggtcttatc tggatgcgtc aaccacacta ctacattggt
     2161 ttgtatccat atacctactc agctggtttg accatcggta cggctatggc taagcaattg
     2221 gaagaacatc ctgaagaagt tgttgaaaaa tggttggaaa ccttgtcact cggtgcaagt
     2281 ctttctgccc aagaccttgc taagcacgca ggtgttgacg tttcaactga ccaaccactc
     2341 aaagaaacca ttgcctatgt tggttcattg gtggataaac tggaaacact aatctaagac
     2401 acataaaaga cttgaacaac ttgttcagg
//
LOCUS       DBNFEG010000208         2404 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.209, whole genome shotgun sequence.
ACCESSION   DBNFEG010000208 DBNFEG010000000
VERSION     DBNFEG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.209"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            214..1128
                     /gene="cas1"
                     /locus_tag="AC1PNG_001986"
     CDS             214..1128
                     /gene="cas1"
                     /locus_tag="AC1PNG_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HIP6702449.1"
                     /translation="MTWRIVHIHQSEKMRLKLDNLVIKKQGDEFTIPLSDISIIVAEG
                     GDTVVTLRLLSALSKYNIALIVCDNHHLPTGIYHSQNGHFRAYKKLQAQLSWAPLQKD
                     KLWQIIIYFKITNQQDVLAMQEKSLSTIQLLSDYREHIELGDRTNREGHAAKVYFNEL
                     FGKQFVRLTQQETDAVNAGLNYGYTIFRAQMARIIAGYGLNALIGIFHKNEYNQFNLA
                     DDLMEPFRQIVDVWVYMNLREADFLTYQHRLELTNLLNAKIRYGKENCSVTVAMDKFV
                     KGFIRCIEDKDTNQFFCPVVSSLEMEKL"
     gene            1125..1448
                     /gene="cas2"
                     /locus_tag="AC1PNG_001987"
     CDS             1125..1448
                     /gene="cas2"
                     /locus_tag="AC1PNG_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HIP6702450.1"
                     /translation="MRYDAMRLLCFFDLPMETTQEKRQYRLFRKELIANGFEMLQFSV
                     YYRTCPNRSFATKFYKKLQQSHLPAGNVRLLAVTEKQFSEMVLIVGGKTRQEEAISDR
                     KLVII"
     gene            1445..>2404
                     /gene="csn2-St"
                     /locus_tag="AC1PNG_001988"
     CDS             1445..>2404
                     /gene="csn2-St"
                     /locus_tag="AC1PNG_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csn2-St"
                     /protein_id="HIP6702451.1"
                     /translation="MKWLITHHVLNNIPIHVGQFTQIVGQNYELKYYIWQILIWYFGG
                     KKYSEEDLILFNQEEPVISQDSEAIKRNAFKIISIAEVGDILEQISYKKGTIGFSYLS
                     LKMQNIEIIEELEILNYHLHKIAQKVNSSISLINDEIEYEVGTTDLLPEQILTKQLTP
                     HFKKSRDEIAIEFISNEKKLCFLLQMLNAIMQEQTKPILLVLKNLDDYLTYDSFVRIA
                     QYLEELSNKYPYFNTILFPSQEGYLYLTEATLETVNIVSDRIEHYPAFTFLYTRYQQS
                     YPSTSPLGEKEFLNSLRKISSYLFSSDINRVVSLADIDLVTLKI"
BASE COUNT          840 a          359 c          469 g          736 t
ORIGIN      
        1 ttataaaaca agagggagaa caccctcaat taaaatttaa aaaataatta aaaaaggggt
       61 tgtatttttt aagtaaaaag tgtaaacttt aactgtaagg gaagccttac actgttactt
      121 aaatcttgca gagcctacaa agataaggct ttatgccgaa atcaagcacc ccgtttcgta
      181 cggggtgctt ttcgttataa acaggagaac accatgactt ggagaattgt acatattcat
      241 caaagtgaaa aaatgcgatt aaagctcgac aacttagtca taaagaaaca aggggatgag
      301 tttacgattc cattaagtga tatttctata attgttgcag aaggagggga tacagttgtt
      361 actcttcgcc tattaagtgc acttagtaag tacaatattg ctttaatagt ctgtgataac
      421 caccacttac ctacaggtat ttatcactct caaaatggac attttagagc ttataaaaag
      481 ttgcaagctc agctatcatg ggcgccgcta caaaaggata aattatggca aattatcata
      541 tattttaaaa ttacgaacca gcaagatgtt ttggcaatgc aggaaaaatc tttgtctacc
      601 attcagttat tatctgatta tagggaacat atcgaacttg gggataggac gaatagggag
      661 gggcatgccg ctaaggtgta tttcaatgag ctgtttggca aacagtttgt tcgactaaca
      721 caacaagaga ctgatgcagt gaatgcgggg ttgaattatg gctacacaat ttttagagct
      781 caaatggcac gaattatagc aggatatggt ttaaacgctt taataggaat ttttcataaa
      841 aatgaataca accaattcaa tttagcagat gatttaatgg agccgtttcg acaaattgtt
      901 gatgtatggg tctatatgaa tttgagggaa gctgactttt taacatacca acatcgtttg
      961 gaattaacaa atctattgaa tgccaagatt agatatggaa aagaaaattg cagtgtcacc
     1021 gtcgcaatgg ataagtttgt caaagggttt attcgttgta tcgaagataa agacacaaat
     1081 caatttttct gtccagtagt ttcaagtcta gaaatggaga aattatgagg tatgatgcta
     1141 tgcgcctgtt atgctttttt gacttgccaa tggaaacgac tcaagagaaa cgtcagtatc
     1201 gcttgtttcg aaaagagttg attgctaatg gctttgaaat gttacagttt tctgtctatt
     1261 atcgtacgtg tccgaatcgg agttttgcaa cgaagtttta taaaaagtta caacaaagtc
     1321 atctaccagc tggtaatgtg agattattag cggtgacgga gaaacaattt tctgagatgg
     1381 tcctaattgt aggtggtaag acaagacagg aagaagcgat aagtgatagg aagttggtaa
     1441 tcatatgaag tggctaataa cacaccatgt attaaataat attccaattc atgttggtca
     1501 atttactcaa attgtcggtc aaaactacga attaaaatat tatatttggc aaatattgat
     1561 atggtatttt ggtggaaaaa agtacagtga ggaagattta atacttttta atcaagagga
     1621 gcctgtgatt tcacaggaca gtgaagcgat aaaaagaaat gcttttaaaa tcatttcaat
     1681 agcagaggtt ggagatatcc tagagcagat tagctataaa aagggaacga ttggattttc
     1741 atatttgtca ttaaaaatgc agaacataga gattattgaa gagcttgaaa tattaaatta
     1801 tcatttacat aagattgctc aaaaagtaaa ttcaagcatt tcgttgataa atgatgaaat
     1861 cgagtatgag gttggaacga ccgatttact tccagagcaa attttaacaa agcaacttac
     1921 tccgcatttc aaaaagtcaa gagatgagat cgctattgaa tttatttcaa atgaaaagaa
     1981 attgtgtttt ctattgcaaa tgttgaatgc aataatgcaa gaacaaacaa aaccaatttt
     2041 actggtactt aaaaatttag atgattacct aacttacgat tcttttgtga gaatagcaca
     2101 gtatttagag gaacttagta ataagtatcc ctatttcaac acaatcttgt ttccttctca
     2161 agagggctat ttgtacttga ccgaggcaac gcttgagact gttaatattg tgtctgatag
     2221 aattgaacac taccctgctt tcacgtttct ctatactcgt tatcagcagt catatccctc
     2281 aactagccca ctaggtgaaa aagaattttt aaactctttg agaaaaatca gttcctattt
     2341 attctcttca gacattaata gagtcgtgag cttagcagac atagacctag taactctaaa
     2401 gata
//
LOCUS       DBNFEG010000209         2358 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.211, whole genome shotgun sequence.
ACCESSION   DBNFEG010000209 DBNFEG010000000
VERSION     DBNFEG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.211"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..1398)
                     /locus_tag="AC1PNG_001989"
     CDS             complement(<1..1398)
                     /locus_tag="AC1PNG_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIP6702452.1"
                     /translation="MNQNLSHYQFSPESQYLDRKSARKKPSELLKHLIAFANADGGQL
                     VIGIEDEKQENIITGFKDGRAYPIDDFKKIDREMRETPLDLSFEEIPVINHKGEEDFI
                     LVISVELSSNRVIAAPNDEVYLRQGDETVKLSYEQRTQLSYDKGQRFFEDEVVPDASL
                     EDIDDSLVQDFKNRFDISERSVEEILKARRFLINGKLTKAAILLFGKYPSAFFPQARV
                     RFQRFDGTDMGTGSSFNVIKEVTFDDALPILIIKVRDFIRTQLREFQYLDDSGQFKIL
                     PEYPEFAWFEGVVNAVTHRDYSVYGDHIRVLMFDDRLEIHSPGKLPNIVTVDNIKHER
                     FSRNPRIARTLTEFGWVREMNEGVKRIYSEMESAFLHEPKFSEPGNKVVLTLENNIVS
                     RHLRTRDSLEKQFTDFGDLNADEQLLVHYMYNSGEKMTTAKAIELTGRSRSFVVKMLH
                     HLRDLEIITWFGSSKN"
     gene            complement(1443..>2358)
                     /locus_tag="AC1PNG_001990"
     CDS             complement(1443..>2358)
                     /locus_tag="AC1PNG_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIP6702453.1"
                     /translation="KLTSSQTEKKSSEMLKKALALLANFFEDYVDFPKLAEFESHENA
                     SPEEVANQIRKNWGLGDSPISSVLRLLETNGFHIAIINSKSEKVDAFGSFVELKMETD
                     SERYYCILIDQDNNNFFRQQFSLAHELGHWALHARTVYPQELSNIEYRQMEQEANRFA
                     SSFLLPASAFSEDLKGHEEDLAYYLKLKSKWNVSIASMIYRAKDLGLLNSEQYTRLQK
                     RMSARGWRREEPEDASIPVSRPILAKQAYKLLTDAGLFEKQSIVQQFNLKYGYPLPIE
                     IFSELMDIPKETLLQERNNIVHLKSRTL"
BASE COUNT          709 a          510 c          394 g          745 t
ORIGIN      
        1 atttttactt gatccaaacc aagtgatgat ttctaaatca cgaagatgat gcaacatttt
       61 aacaacaaac gagcgacttc ttcccgtcag ctcaatggct tttgcggtgg tcatcttctc
      121 accagaattg tacatatagt gaaccagcaa ttgttcatca gcattgaggt ctccaaaatc
      181 agtaaactgt ttctccaaac tatctcgagt ccgtaggtgt cggctgacaa tattattttc
      241 aagcgtcagg acaaccttat tcccaggttc tgagaattta ggttcatgga gaaaagcaga
      301 ttccatctca gaatagatac gctttactcc ttcattcatc tcacgtaccc aaccgaactc
      361 tgtcaaggtt ctggcaatgc gagggtttcg tgaaaaacgt tcatgcttga tattgtcaac
      421 ggtgacgata ttgggtaatt tccctgggct atgaatctct agtcgatcat caaacataag
      481 cacacgaatg tgatcgccat agacggaata atcacggtgc gttacagcat tgacaacacc
      541 ttcaaaccaa gcaaattcag gatactcagg taaaattttg aattgcccac tgtcatccaa
      601 atactgaaat tcccgaagct gggtgcgaat aaaatctcta accttgataa ttaagatcgg
      661 tagcgcatca tcaaaggtca cttccttgat aacattaaaa ctacttccag ttcccatatc
      721 tgtaccatca aatcgctgga atcggacacg cgcttgaggg aagaatgctg acggatattt
      781 gccaaagagt aaaattgctg ccttggttaa tttcccattg attagaaatc gacgtgcttt
      841 aaggatttct tccacagaac gctctgatat atcaaaacga tttttaaaat cctgaaccaa
      901 gctatcatca atatcttcca gactggcgtc tggcaccact tcatcttcaa agaaacgttg
      961 tcccttgtca tagctgagct gagtgcgttg ctcgtagctc aacttgactg tttcatcacc
     1021 ctgacgaaga taaacctcat catttggcgc ggcaatcact cggtttgacg acaattcaac
     1081 tgagataact aaaatgaaat cttcttcacc cttatgattg attacaggga tttcctcaaa
     1141 agataaatcc aaaggagtct cacgcatctc acgatcaatc tttttgaaat cgtctatggg
     1201 ataagccctg ccgtccttga aaccagtgat aatattttcc tgtttctcgt cctcaatacc
     1261 aataactagt tgaccgccat ctgcattggc aaaagcaatc aagtgtttga gaagttcaga
     1321 cggtttcttt ctggctgatt ttcgatctag gtattggctt tctggagaaa attgatagtg
     1381 agataagttt tgattcatat agttctcctt aaatatataa aaaatactta acaattcatg
     1441 atttacaaag tcctagattt taaatgtaca atattatttc tctcttgtaa caacgtctcc
     1501 tttggaatgt ccataagctc tgaaaatatc tctattggaa gaggatagcc gtacttgaga
     1561 ttaaattgct ggactatgga ttgtttttca aataagcccg catccgtcag taatttatat
     1621 gcctgtttag caaggatcgg tcgtgatact ggaatacttg catcctctgg ctcttctctt
     1681 cgccatcctc tggcactcat tcttttttgt agacgagtat attgttccga gtttaacaaa
     1741 cccaaatcct ttgcacgata aatcatactg gctatagata cattccactt agacttcaat
     1801 ttcaaataat atgctaaatc ttcttcatgt ccttttaaat cttcagaaaa agcagaggct
     1861 ggtaataaaa aactcgaagc aaatctatta gcttcttgct ccatttgtct atattcaata
     1921 tttgaaagct cctgtggata tactgttctt gcatgcaaag cccaatgccc taactcatga
     1981 gccaagctaa attgttgcct gaaaaaatta ttattatctt ggtcaattaa aatacaataa
     2041 tatctctcag aatctgtttc cattttcaat tcaacaaaac ttccaaaagc atccactttc
     2101 tcagactttg aattaattat agcaatatgg aatccattcg tttctagtaa cctaagaaca
     2161 ctggaaattg ggctatcacc taatccccaa ttctttctta tctgatttgc aacttcctct
     2221 ggtgaagcat tttcatgact ttcaaattct gctaatttcg ggaaatcaac ataatcttca
     2281 aaaaaattcg ccaagagagc cagtgctttt tttagcatct cactagactt cttctcagtt
     2341 tgtgaggatg ttaactta
//
LOCUS       DBNFEG010000210         2356 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.212, whole genome shotgun sequence.
ACCESSION   DBNFEG010000210 DBNFEG010000000
VERSION     DBNFEG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.212"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(91..2268)
                     /gene="yzpA"
                     /locus_tag="AC1PNG_001991"
     CDS             complement(91..2268)
                     /gene="yzpA"
                     /locus_tag="AC1PNG_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing zinc-binding protein
                     YzpA"
                     /protein_id="HIP6702454.1"
                     /translation="MNSKIFSLRKSKMGLVSVAIAFLWIGTGMNMETAMAEETDATAL
                     ETQLESKESSLTNTVSENAEAEEVTDEVPSEEKKSEEMEDMEEELPFIENHLEVAPIQ
                     AGDQTISGNTTPGGYVAITIDGEAITSIENILEADDKGDFSYRLSKPLAHSQTVEISA
                     LPKQFWTLEADSEERKVVVRTNRHPEAYEIPAKRLEKTSNGMHQVFIEPVFEHTSKVI
                     GHTSVKGSVYLSINGSFVSDKTLIDPKDGRFEVTFSESLAGSKFKADDRLVLSFVSED
                     GQPVITNTIVKPLVKEKVSSQMTVKPLSSATSVLEGTTFPLGRVHLYNADTSEFIMEA
                     IADETGHYKIALPALQSEDKYYRLTHNQQEDLVSVHLDTVDGSSILLDKSVMASLATY
                     LQDADMDEATDEDPIIVPKLHNKKDYIVGRTIHLNAYVRMVSSIKGKQYPPVQVDELG
                     FFGFQIQDLQLPFEKGERIRFEIIDPVTNNIIASKEEVVGQYLEDEDVMDLPFQVEKV
                     TTDHGYISGKTAPDVMIELVSTQNGEEIIGKTSTDSTGRFEFDLGSRVLKNGETLSFR
                     AFDKEGEQVAWEVVTVQKGNGHRINKPDKKDEKEEQPSKEITKNIEQSDTLEQTTLPP
                     VRQTLTDKKVEQNAEPSKEETVSIFDDSKKDMPTKQEKMARTVRDKGTKGNVSVHDSG
                     ENTQVQSLPKTGEKTSLVANIMLSIILFLFALFIGKKKITESE"
BASE COUNT          656 a          513 c          363 g          824 t
ORIGIN      
        1 aaagaaaaat tttaactctt ttaatttaaa gctacacaat gcctctcatc tgtcgcaaac
       61 gaatttttga gagagctttg tcatcgcctt ttactcactt tctgttatct ttttcttgcc
      121 aatgaataaa gcaaataaga ataatatgat cgataacatt atattggcaa ccaagctcgt
      181 tttttcaccg gtcttaggca ggctttgaac ttgggtattc tcaccactat catgcacaga
      241 tacgtttcct tttgtccctt tgtctcttac agtacgagcc attttctctt gcttagtagg
      301 catatctttt ttcgaatcat caaaaataga aaccgtttct tcttttgatg gctctgcatt
      361 ttgctctact tttttgtcag tcagcgtttg gcgtacaggt ggcaaagtag tttgctcaag
      421 tgtgtctgat tgttcgatat ttttggtaat ttctttgcta ggttgctctt ccttctcgtc
      481 ctttttatct ggtttattaa tgcggtgacc atttcctttt tgaaccgtta cgacttccca
      541 agctacctgt tcaccttctt tatcaaacgc tctaaacgat aaggtttctc cattttttaa
      601 aacacgacta cccaaatcaa attcgaatcg acctgttgaa tcagttgaag ttttaccaat
      661 tatctcctcg ccattctgag ttgatactag ttcaatcatt acatctggag ctgttttgcc
      721 actgatgtaa ccatgatctg tagtgacttt ttctacttga aatggtaaat ccatcacgtc
      781 ttcatcttcc aaatattgcc ctacaacttc ttccttacta gcgatgatat tatttgtaac
      841 cgggtcaatg atttcaaagc gaattctctc acctttttca aaaggcaatt gtaaatcttg
      901 aatttggaaa ccaaaaaatc ctaattcgtc cacttgaact ggaggatact gttttccttt
      961 gattgacgaa accatgcgaa cgtaggcatt aagatgtatc gttcgaccga caatataatc
     1021 ttttttatta tgcagtttag gtacaatgat aggatcttca tctgtcgctt catccatatc
     1081 tgcatcttgt aaataagttg ctaaagatgc cataacagat ttatccaata atatacttga
     1141 accatcaact gtatcaagat ggacagatac caagtcctct tgttgattgt gagtcaatct
     1201 gtagtactta tcctcggatt gtagggcagg caaagcaatc ttatagtgac ctgtctcatc
     1261 agcaatagct tccatgataa actctgaagt atcagcattg tatagatgca cacgacctaa
     1321 aggaaatgta gttccttcca aaactgaagt cgcacttgat aaaggtttta ctgtcatttg
     1381 tgaagatact ttttctttta ccaatggttt tacaatagta ttagtaatca ccggctgacc
     1441 atcttctgat acaaaagaca aaaccaatct atcatctgct ttaaattttg aacctgctaa
     1501 actctcggaa aaagtaacct caaaccgtcc atctttagga tcaattagtg tcttatcaga
     1561 gacaaaacta ccatttattg atagatagac tgaacctttg acagaggtat gaccaataac
     1621 tttgctcgta tgttcaaaaa ctggttcgat gaacacttga tgcattccat tagatgtttt
     1681 ttctaatctt tttgctggaa tttcataagc ctctggatgt cgattagtcc tcactactac
     1741 cttacgctct tcagaatctg cctctagagt ccaaaactgt ttcggcaaag cactaatttc
     1801 cacagtttgg ctgtgagcaa gtggtttact caaccgataa gaaaaatctc ccttatcatc
     1861 tgcttccaaa atattctcaa tagaagtaat tgcttcccca tctatcgtaa tcgctacata
     1921 tccaccagga gtggtattcc ctgagatggt ttgatctcca gcttgaatcg gagcaacctc
     1981 tagatggttt tcaataaatg gaagttcttc ctccatatcc tccatctctt ccgatttttt
     2041 ttcttcactc ggtacctcat cagtcacttc ctcagcttca gcattttccg aaactgtatt
     2101 ggtaagcgat gattccttgc tttctaattg agtttcaaga gctgtagcgt cagtctcctc
     2161 agccattgcg gtttccatat tcatccctgt accaatccat agaaaagcaa ttgccaccga
     2221 aactaaaccc atcttacttt ttctcaacga aaaaatcttg ctattcatct tttctcctta
     2281 tatctgtatt aataattaac gacacatatt gtcctagttt tattataaaa aaaacatctc
     2341 atagaacatt gttttt
//
LOCUS       DBNFEG010000211         2353 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.213, whole genome shotgun sequence.
ACCESSION   DBNFEG010000211 DBNFEG010000000
VERSION     DBNFEG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.213"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(48..2066)
                     /gene="recG"
                     /locus_tag="AC1PNG_001992"
     CDS             complement(48..2066)
                     /gene="recG"
                     /locus_tag="AC1PNG_001992"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIP6702455.1"
                     /translation="MKRLCDELSVLPGIGPKSAEKFLKINIQNINDLLTYYPFRYEDF
                     ESKSIYDLQDGEKAVVVGEVVSPANVQYYGYKRNRLRFSMKQGEVVLAVSFFNQPYLA
                     DKIVLGQDIAVWGKWDKAKASLTGMKILAQVSDELQPVYHVAQGISQVNLVKAIKTAI
                     DQGYLHLLEENLPSVLRERYRLMNRREAVFAMHFPTNLEEYRQALRRMKFEELFYFQL
                     QLQMLKANNRDISNGLKIAYDADRLAMQIRQLPFVLTDAQSGALAEILSDMKSYGHMN
                     RLLQGDVGSGKTVVAGLAMFAAVTAGMQAAIMVPTEILAEQHFESLRQLFPELSIALL
                     TGGMKAAERRTALEAISSGQVDIIVGTHALIQESVTYHKLGLVVTDEQHRFGVKQRRL
                     FREKGDNPDVLMMTATPIPRTLAITAFGDMDVSIINQLPAGRKPIITRWVKHQQLPTV
                     LDWLERELEVGAQVYFISPLIEESEALDLKNAVDLQSDLQAHFGEQVTVDLLHGKMKN
                     DEKDAIMQAFKERKTNILVSTTVIEVGVNVPNATVMVIMDADRFGLSQLHQLRGRVGR
                     GHKQSYAVLVANPKTESGKERMKIMTETTDGFILAEADLKMRGSGEIFGTRQSGLPEF
                     QVANIIEDYPILEEARRVASQIVSVENWQEDPNWSILLANLKDREELD"
     gene            complement(2185..2256)
                     /locus_tag="AC1PNG_001993"
     tRNA            complement(2185..2256)
                     /locus_tag="AC1PNG_001993"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2221..2223),aa:Glu,seq:ttc)
     gene            complement(2279..2351)
                     /locus_tag="AC1PNG_001994"
     tRNA            complement(2279..2351)
                     /locus_tag="AC1PNG_001994"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2316..2318),aa:Asn,seq:gtt)
BASE COUNT          744 a          608 c          362 g          639 t
ORIGIN      
        1 caatatgtca aaagaccaac acaggtgtgt tggccttttt tatatgacta gtccaattcc
       61 tctcgatctt tcaaattagc aagcaaaatc gaccaattag ggtcctcctg ccaattttcc
      121 acgctaacaa tctgactagc aactcgtctt gcttcctcta aaattggata gtcctcgata
      181 atatttgcaa cctgaaattc tggcaaacct gattgacgag tcccaaatat ttctccagac
      241 ccacgcattt ttaagtctgc ttcagccaaa ataaaaccat ctgtcgtctc tgtcataatt
      301 ttcatgcgtt ccttaccaga ctctgtttta ggattagcaa ctaacactgc ataagactgc
      361 ttatgaccac gtcctacacg ccctctcaac tgatgtaatt ggcttaaacc aaaacgatct
      421 gcatccataa taaccatcac cgttgcattg ggcacattga ctccgacttc aataacagtg
      481 gtagaaacca aaatattcgt ttttcgctcc ttaaaagcct gcatgatggc atctttctcg
      541 tcattcttca tcttaccgtg taacaaatcc actgtaactt gctctccaaa atgcgcctgt
      601 aaatcagatt gcagatctac agcattcttt aaatctagag cctcagattc ttcaattagc
      661 ggagagataa aataaacctg agcacccact tctaactccc gctccaacca atctaagacc
      721 gtgggcagct gttgatgttt gacccagcga gtgataattg gcttccgacc agcaggtaac
      781 tgattaataa tcgatacatc catatctcca aaagctgtaa tggctaatgt ccgaggaata
      841 ggtgtagcag tcatcataag cacatccgga ttatcaccct tctcacgaaa cagacggcgc
      901 tgtttaacac caaaacgatg ttgctcatcc gtcacaacca aaccaagctt atggtacgtc
      961 acactttcct gtataagagc atgagttcct acaatgatgt ctacctgacc actagaaatc
     1021 gcctctaaag cagtacgacg ctccgcagcc ttcattccac ctgtcaaaag agcaatagaa
     1081 agttctggaa aaagctggcg caaactctca aagtgttgct cagccaaaat ttctgtcggc
     1141 accataattg cagcctgcat ccccgcagtc acagcagcaa acatagccaa tccagcaact
     1201 actgtcttcc ctgaaccaac atccccttgt aacaaacgat tcatgtgccc ataggacttc
     1261 atatctgata gtatttcagc caaagcccca gactgagcat ctgttaagac aaacggcaat
     1321 tgtcttattt gcatagccaa tctatcagca tcataggcaa tcttcaaacc attcgaaata
     1381 tcacgattgt tggctttcag catttgcaac tgtaattgaa aataaaataa ttcctcaaac
     1441 ttcatacgtc gcaaagcttg tctatactct tctaaatttg taggaaaatg catcgcaaaa
     1501 accgcctctc ggcgattcat taaccgatag cgctctcgta aaactgatgg caagttctcc
     1561 tctaataaat gcaaatatcc ctgatcaata gctgttttaa tagccttaac caaatttact
     1621 tgcgaaatcc cttgcgccac atgatagaca ggttggagct catctgatac ttgagccaaa
     1681 attttcattc ctgtcaaact tgccttagcc ttatcccact ttccccaaac tgcaatatcc
     1741 tgccccaaaa caatcttatc tgctaaataa ggctgattaa aaaacgaaac tgctaagaca
     1801 acctcaccct gtttcattga aaaacggagt ctatttcttt tgtaaccata atactgtaca
     1861 ttagctggag ataccacctc cccaactacc actgccttct ccccatcctg caaatcataa
     1921 atagacttac tctcaaaatc ttcatatcta aatggatagt aagtcagcaa atcattaata
     1981 ttctgaatat taatttttaa gaatttttca gccgattttg gaccaatccc tggcaaaacc
     2041 gataattcat cacataatct tttcatgtct ctattatatc aacaaacaat gaatagacaa
     2101 agtaacaaga aaagaaaaaa gagccaaatt aaagcccata cacctcaaaa aacaaaaaaa
     2161 agagacctaa gcagcctcca aatatagtcc gtacgggatt cgaacccgtg ttaccgccgt
     2221 gaaaaggcgg tgtcttaacc ccttgaccaa cggaccatat actaaagatt tctctttact
     2281 ccgccaacag ggctcgaacc tgtgacatca tgattaacag tcatgcgctc taccaactga
     2341 gctatggcgg aat
//
LOCUS       DBNFEG010000212         2312 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.214, whole genome shotgun sequence.
ACCESSION   DBNFEG010000212 DBNFEG010000000
VERSION     DBNFEG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.214"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1607
                     /locus_tag="AC1PNG_001995"
     CDS             <1..1607
                     /locus_tag="AC1PNG_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIP6702456.1"
                     /translation="EKVKAIYTKTKIHMPTLEKDNHLIDIDPFTIFGLFNKQIKEENR
                     IKILTAIAELFNLQTEIPSSFESIPVLMNQNATFYYFIGDRDEKDIDDLWELFIAALT
                     YSKEPTAEKKEKFSHYFDLAINKKGNGNSKITMALYWIAPDFFLNLDSRNKWYVYESG
                     EIPTDVIKKLPKMKSKISADDYFYISDSMHHYLNSAESKLKDFKELSSMAWKYSKQIN
                     QEEKERSAGAISPLADETDTGNEIGSEEVVEDSYTKNHFLSEVYMTEEEYDQLTSILQ
                     MKKNIILQGAPGVGKTFVAERLAFSLMGKPDLERVMTIQFHQSYSYEDFIMGFRPSTS
                     GFELRRGAFYTFCKKAESDKSHDYFFIIDEINRGNLSKIFGELFMLIENDKRGKSLQL
                     LYSDEQFSIPENLYIIGMMNTADRSLALLDYALRRRFAFFDIKPGFSTAGFKDYQTNL
                     KNEKFDRLISCIEKLNQAISTEEVLGDSFCIGHSYFCGLSAEVLDQGLLEAIVEYELI
                     PLLKEYWFDEPTKIHDWSSKLRSAIQ"
     gene            1604..>2312
                     /locus_tag="AC1PNG_001996"
     CDS             1604..>2312
                     /locus_tag="AC1PNG_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methylcytosine restriction system specificity
                     protein McrC"
                     /protein_id="HIP6702457.1"
                     /translation="MIPVQNIYYMLSYAFKILHKQEYKRIATEEFKNAADLLAEIMII
                     SLSIQVKRGLGREYRSQTESLSALKGKINISESLTPPNWRRKQLVCQYDDFSLDSRMN
                     RIIKASIEILLKADISRDRKKKLRKLLVFFGEVSKINLHSINWNLQYNRNNQSYQLLM
                     SICYLVVNGLIHTEREGNKKLMNFLDERRESLLYEKFILGYYKKHYPQIQVTASQIPW
                     ALDDGFGEMLPIMQSDIY"
BASE COUNT          801 a          399 c          464 g          648 t
ORIGIN      
        1 ttgagaaagt aaaagctatc tataccaaaa caaagataca tatgcccacg ttagaaaagg
       61 acaatcacct gatagatatt gatcctttta cgatatttgg actcttcaac aagcaaataa
      121 aagaggagaa tcgcattaaa attctgacag ctatagcaga attattcaac ctccaaacgg
      181 aaatcccatc ctcttttgaa agtataccag tactgatgaa tcagaatgcg actttctatt
      241 attttatcgg agatcgtgat gagaaagata ttgatgactt atgggaactt ttcattgctg
      301 ctttgaccta tagcaaggag cctacggctg agaagaaaga aaaattttca cactattttg
      361 atttggctat aaacaagaaa ggaaatggca acagcaagat tacgatggcc ttgtattgga
      421 ttgctcctga ctttttcttg aacttagata gcagaaataa gtggtatgta tacgaatctg
      481 gggagattcc tactgatgtc atcaaaaaac taccaaagat gaagtcaaaa atttctgccg
      541 atgattattt ttatatctcg gactctatgc atcattattt aaacagtgca gagagcaaac
      601 tgaaagattt taaagaatta tcatccatgg cttggaaata ctctaaacag atcaatcaag
      661 aagagaagga aagaagtgct ggagcaattt ctccactggc tgatgaaact gatactggaa
      721 acgagatagg tagtgaagag gtagtagaag atagctatac gaaaaatcac ttcctatcag
      781 aagtctatat gacagaagaa gagtatgatc aattaacctc catactccaa atgaaaaaga
      841 atatcatctt acaaggagcg ccaggagttg gaaagacgtt tgttgcagaa cgcctagcat
      901 tttcattaat gggtaaacca gatttagaaa gggtcatgac aattcagttt caccaaagct
      961 attcctacga ggacttcatc atgggcttta gaccttcgac aagtggcttt gagctgagaa
     1021 gaggggcatt ctatactttc tgcaaaaaag cagagtctga caagagtcat gattatttct
     1081 ttattatcga tgaaataaac aggggaaatt tgagcaaaat ttttggtgaa ttgtttatgc
     1141 tgatagaaaa tgacaaacga ggcaagtctt tacagctcct ctactcggat gagcaattct
     1201 caattccaga aaatctatat atcatcggca tgatgaatac cgcagatagg agtttagccc
     1261 tacttgatta tgcactgaga cgacgctttg ccttctttga catcaagcca ggtttttcaa
     1321 ctgcgggatt caaagattat caaacaaatc tgaaaaacga aaaattcgat agactcattt
     1381 cctgtataga aaagctaaat caggccattt caacagagga agtactggga gatagcttct
     1441 gtattgggca tagttacttc tgcggtttat cggcagaagt actggaccaa ggtttgctag
     1501 aagccattgt tgaatatgaa ctgattcccc tcttgaaaga atattggttt gatgaaccga
     1561 caaaaattca tgattggagt agtaagttaa ggagtgccat ccagtgatac ctgtccaaaa
     1621 tatctactat atgctatcct acgcattcaa aatccttcat aagcaggaat ataaacggat
     1681 cgctactgaa gaatttaaaa atgctgcaga tttactggcg gaaataatga taatcagcct
     1741 ctcgatacag gtgaaaagag gacttggtcg agagtatcgt tctcaaaccg agtcactctc
     1801 ggcacttaaa ggtaaaataa atatctctga atctttaaca cctccgaatt ggcgtagaaa
     1861 gcaacttgtt tgccaatacg atgatttttc tcttgacagt agaatgaatc gcattatcaa
     1921 agcaagcatt gagatactgc taaaagctga tataagcagg gacaggaaaa agaaattgag
     1981 aaaactctta gttttctttg gagaagtctc aaaaattaat ctgcactcaa tcaattggaa
     2041 tttacaatat aatcgaaaca atcagtcgta tcaattacta atgtcaatct gctatttggt
     2101 ggtgaatgga ttaattcata cagagaggga ggggaataag aaattgatga attttcttga
     2161 tgagcggagg gaatccttgc tatatgagaa gttcattctt ggatattata aaaagcatta
     2221 tccccaaata caggttaccg cctctcagat accttgggca ctagatgatg gttttggaga
     2281 aatgctcccc atcatgcaga gtgatattta cc
//
LOCUS       DBNFEG010000213         2190 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.215, whole genome shotgun sequence.
ACCESSION   DBNFEG010000213 DBNFEG010000000
VERSION     DBNFEG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.215"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            26..763
                     /locus_tag="AC1PNG_001997"
                     /pseudo
     CDS             26..763
                     /locus_tag="AC1PNG_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713635.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(814..2163)
                     /gene="gorA"
                     /locus_tag="AC1PNG_001998"
     CDS             complement(814..2163)
                     /gene="gorA"
                     /locus_tag="AC1PNG_001998"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HIP6702458.1"
                     /translation="MKVFDIIAIGGGSGGIATMNRAAMYGAKAAVIEGSYLGGTCVNL
                     GCVPKKIMWYGSQVAEAIQHYGPEYGFTSQEVTFDFATLRKNREAYIERSRASYGNTF
                     NNNGVEVIQGFARFVDNQTVEVNGELIRAKHIVIATGAKPVVPAIPGSELGIVSDDVF
                     AWEELPSSVAVIGAGYIAVEMAGLLHGLGVQTDLFVRGNRPLRSFDTYIVDALMEEMQ
                     RTNLPLHTGKTPVSLEKTDDGQIQINFADGSNHIAQKVLWAIGRKPNIDKLNLKATSV
                     QLTSSGHISVNEYQETAVPGIYALGDVTGEKELTPVAIKAGRLLAERLFNNKPTAKMD
                     YTTIPTVVFSHPAIGTVGLTEDEAITQYGQDNVKVYTSAFTSMYTALENNRQMAKFKL
                     VTVGENEKVVGLHGIGYGVDEMIQGFSVAIKMGATKEEFDAVVAIHPTGSEEFVTMR"
BASE COUNT          632 a          464 c          461 g          633 t
ORIGIN      
        1 ataaaaaaag aaagtgagaa tttctatgac agaattactt gatatttacc gtactcttaa
       61 atctaaaact tgggtagact tgactcacca aatcaatgaa aagagcccga atttccctgc
      121 gctaccagct ctcgaaaaga aagccctatt tactcataag gatggcttct ttgtcgaaca
      181 atttacggtt gtggggcagt acggaaccca tcttgatcct ccgattcatt ttgtagaagg
      241 ggctcgttat cttgatgaga ttgatttgaa agatttactc ctgccactct acgttattga
      301 taaatcagct gctgttatag aaaataatga ctatgagata actaagcagg atattttaga
      361 ctttgaggct gaatatggtc cgatagcgcc aaaatctttt gttgcctttc gttcggattt
      421 gtcaaagcgc tggcccagtc aagatgccat tcgtaatcta gacgaagatg gagttcaacg
      481 tacaccagga tggagtcatg aagctctaga atatttgatt gaagaacgtc aggtgaaagc
      541 agttggtcat gaaacattgg ataccgattt aggagtatct gcagccaaac acggaggaag
      601 tttatcagaa gaatactatc tcctatcaaa agatatttat cagttggaag tattggcaaa
      661 cttggattag gtaccgccaa caggtgcatt gatttcaatc gcttttcctc attgggagaa
      721 ggcttcgggt tcaccagtgc gagcaattgc catattacca taaaaaaaag tccagacagt
      781 gctgaccttc atgtctggac tttttcaatt gagttaacgc atagtaacaa attcttcaga
      841 acctgtcgga tggatggcga caacggcatc aaattcttct tttgtagctc ccattttgat
      901 agcaactgag aatccttgaa tcatttcatc gacaccatag ccaatgccgt gtagtccaac
      961 gactttttca ttttctccca cagttactaa tttaaactta gccatttggc gattgttttc
     1021 tagggcagtg tacatagaag taaaggctga agtatagact tttacattgt cctgaccgta
     1081 ttgggtaatc gcttcgtcct cagttagacc aacggttccg atagcggggt gagaaaagac
     1141 gacagttggg atggttgtgt aatccatttt tgcggttggt ttattgttaa ataaacgttc
     1201 tgccaaaagg cgacctgcct tgattgcaac gggtgttaat tctttttcac cagttacatc
     1261 accgagggca tagatgcctg gtacagctgt ttcttggtat tcattgacag aaatatgtcc
     1321 tgaagaagta agttgaacac tagttgcttt caggttgagt ttgtcaatat ttggtttacg
     1381 tccaattgcc caaaggacct tctgagcaat atggttacta ccgtcagcaa agttaatctg
     1441 gatttgtcca tcatcagttt tttcaagact gacaggagtt ttcccggtat ggagtggaag
     1501 gttggtacgt tgcatttctt ccatcaaggc atcaacaatg taggtatcaa agctacgaag
     1561 agggcgatta ccacgaacaa ataggtcagt ttggacaccg agtccatgta ggagaccggc
     1621 catttccaca gcgatataac cagcaccaat cacagccaca gaagagggta attcttccca
     1681 tgcaaagaca tcgtcagata caatccctag ctcactacca ggaatagcag ggactaccgg
     1741 tttggcgcca gttgcgatga caatgtgttt tgcacgaatc agttcaccat tgacttcaac
     1801 cgtttgatta tccacaaaac gtgcgaaacc ctggataact tctactccgt tattgttaaa
     1861 agtattaccg taggatgcgc gtgaacgctc aatataggct tcgcgatttt tacgcaaggt
     1921 ggcaaaatca aaggtcactt cttggctagt aaatccatat tcaggtccat aatgctgaat
     1981 ggcttctgct acctgagagc cgtaccacat aatttttttg ggtacacagc caaggttaac
     2041 acaagtccct cctagatagc ttccctcaat aactgctgcc ttggcaccgt acatagcagc
     2101 tcggttcatg gtagcgatgc ctccgctccc accaccgata gcgataatat caaacacttt
     2161 cataaatttc ctccaataaa atcattaact
//
LOCUS       DBNFEG010000214         2184 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.216, whole genome shotgun sequence.
ACCESSION   DBNFEG010000214 DBNFEG010000000
VERSION     DBNFEG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.216"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            59..>2184
                     /locus_tag="AC1PNG_001999"
     CDS             59..>2184
                     /locus_tag="AC1PNG_001999"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004557 - alpha-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HIP6702459.1"
                     /translation="MNVIEIYNEKQIFHLKTREFSYIIQVLETGDLVHRYFGKKIEKF
                     SDGNKITYLDRSFSPSPITGDRTYSLDVLPLEYSSNGLGDFRTSALDVRNEFGVTLDL
                     KYKEYRLYKGKKELRGLPASFGNQEEVESLEIDLYDQLTDVTVTLQYSVFEEASYLAR
                     SATIQTGKYPCKLEKVLSATLDFPHQDFIVHSLAGRYAYEKEWTQTPLTKGQYSIGSI
                     RGASSHSRTPFLALASPDASEDKGDVYAAHLVYSGNFTAFVETTAMETSRLGLGLESH
                     YFSWQLDKDDRFQTPEVLLSYTDKGFTGMTQNSHHFITKHLIRSSFVNKPRPILINNW
                     EATYFEFTEEKILQLAQVASRAGIELFVLDDGWFGKRNNDESSLGDWKVNLDKLPNGL
                     NGLADRINELGMKFGLWFEPEMISIDSDLYREHPDWAIRTEGRLPIYSREQLVLDLTK
                     QEVCDYIIDSVSSILESANISYVKWDMNRNITNIPEGLANDQRFEFHHRYMLGLYRVL
                     DHLTKRFPDILFESCAGGGGRNDLGIMYYMPQAWASDDTDAIERLSIQEGTSLIYPPS
                     SIGAHVSAVPNHQVGRITPLATRGNVAMMGGAFGYELDLTKLSEKELDEISQQIETYH
                     SIRETIQFGQLYRLKKTSNTWAANYVGQDKNQVVFTFVKILAKPEAPLLYVRLKGLDP
                     AALYECPQLGETFYGDELMNIGLTMP"
BASE COUNT          671 a          420 c          476 g          617 t
ORIGIN      
        1 ccattaaata aagcgttttc attttgtaaa ataatagcat aaagaagaga tgggagagat
       61 gaatgtgatt gagatttata atgaaaaaca gatttttcac ctgaaaacaa gagaattctc
      121 ttatatcatc caagtgttgg aaacaggcga tctggttcat cgttattttg ggaaaaaaat
      181 cgaaaaattt agcgatggaa acaaaataac atatttagat cgatcgtttt ctccgagtcc
      241 aattactggg gataggacat actcattgga tgtcctccca ctcgaatatt ccagcaatgg
      301 tctgggagat tttagaacct cagcattaga tgttcggaac gaatttggag tcactcttga
      361 cctaaaatac aaagagtatc gattatacaa ggggaaaaaa gaactgaggg gattaccagc
      421 aagttttgga aatcaagaag aggtagagag tcttgaaatt gatctttacg atcagttgac
      481 agatgttacc gtaacacttc agtattctgt ttttgaagaa gcttcctatc tcgcacggtc
      541 agctacaatt caaactggca aatatccatg caaactagag aaggtcttgt ctgcaacgct
      601 tgattttcca catcaagatt tcattgttca cagcttggca ggacgttatg cttatgaaaa
      661 agagtggaca cagaccccat tgacaaaagg tcagtattcg attggtagca ttcgaggtgc
      721 ttcaagtcac tcaagaacac ccttcttagc actagcctca cccgatgcaa gtgaggacaa
      781 gggcgatgtt tatgcagccc accttgtcta tagcggcaac tttacagcat ttgtcgaaac
      841 gacagccatg gaaaccagtc gattgggact aggattggaa agtcactatt tctcatggca
      901 attggataag gatgaccggt ttcaaactcc agaagttctc ttatcataca ctgataaagg
      961 atttactggt atgacacaaa atagtcatca ctttatcaca aaacacctca ttcgatcatc
     1021 gtttgtaaac aaaccaaggc ccattttaat caacaactgg gaagcaacct attttgaatt
     1081 tacagaagaa aagattttac agttagctca ggttgcaagt cgagctggta ttgaattgtt
     1141 tgttctggat gatggttggt ttggtaaacg aaacaatgat gaaagttcat tgggcgattg
     1201 gaaagtcaat ttggataagt tgccaaatgg tttgaatggt ttagcagacc gaataaatga
     1261 gcttggcatg aaatttggac tgtggtttga accagagatg atttctattg atagcgatct
     1321 ttatcgtgag catcctgatt gggcaattcg cacagaagga cgcttaccaa tctacagccg
     1381 agaacaatta gttttggatt tgaccaagca ggaagtctgt gactacatca ttgatagcgt
     1441 ctcttccata ttagaatcag ccaatatttc ttatgtaaaa tgggatatga accgtaatat
     1501 caccaatatc cctgaaggtt tagcaaatga ccagcgcttt gagttccacc atcgttacat
     1561 gctaggtctc taccgtgtat tagaccacct cacaaagcgg ttcccggata ttcttttcga
     1621 atcctgtgca ggcggtggag gtcgtaatga tttgggaatc atgtattaca tgccacaagc
     1681 ttgggcaagt gatgatacag atgcgattga gcgcttatct attcaagaag gtactagttt
     1741 gatttatccc ccttcctcaa ttggtgcaca tgtttctgcc gttccgaacc atcaggttgg
     1801 tcggatcaca cctcttgcta cacgaggcaa tgtggcaatg atgggaggag catttggtta
     1861 cgagctggat ttaacgaaac tttcggaaaa ggaattggat gaaatcagtc agcaaatcga
     1921 aacctatcac tccattcgtg aaactatcca attcggtcag ctctaccgtc tgaaaaagac
     1981 gagcaatacc tgggctgcca attatgttgg tcaagataag aatcaagtgg tctttacatt
     2041 tgtaaaaatt cttgccaagc cagaagcgcc tttgctttat gttcggttga aggggctaga
     2101 cccagctgcc ctatatgaat gccctcaatt aggggaaact ttctacgggg atgaattgat
     2161 gaacattgga ctcaccatgc ccca
//
LOCUS       DBNFEG010000215         2174 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.217, whole genome shotgun sequence.
ACCESSION   DBNFEG010000215 DBNFEG010000000
VERSION     DBNFEG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.217"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(23..751)
                     /locus_tag="AC1PNG_002000"
     CDS             complement(23..751)
                     /locus_tag="AC1PNG_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIP6702460.1"
                     /translation="MKKDFFVIGDVHGKFELLLDILKKWNEERQQLIFLGDLIDRGEN
                     SKACLELVWQLVREKDAICLTGNHERMFLAWLRDPLERYDHYRRNGGDTTINSLLGRP
                     LDAPVDGLVDANAVMEQYEKLIDFVKSCPYHLETEGYIFVHAGVDLTLPDWRKTSNHD
                     KVWLRTPFHEAENTTGKMIVFGHTPVYNLYQTKLRISQIWTSSDGKMGIDGGAVYGGV
                     LHGLVLNQQGLVRDYAVGCMESTQ"
     gene            complement(817..2166)
                     /locus_tag="AC1PNG_002001"
     CDS             complement(817..2166)
                     /locus_tag="AC1PNG_002001"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIP6702461.1"
                     /translation="MTHITFDYSKVLGQFVGAQEVEYMQPQVTLADQMLRQGTGPGSD
                     FIGWLDLPENYDKEEFARIKEAAAKIQNESEVLVVIGIGGSYLGAKAAIDFLSNAFVN
                     LQTREERKAPQILYAGNSISSSYLADLVDYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKAKGAVKVEADANGWETFVVPDDVGGRFSVLTAVGLL
                     PIAAAGADIDALMEGANAARTTFTSDKIAENQAYQYAAVRNILYRKGYVTEILANYEP
                     SLQYFSEWWKQLAGESEGKDQKGINPTSANFSTDLHSLGQFIQEGNRNIFETVVRVDK
                     PRKNILIPDMAEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPQQDEF
                     TLGYTFYFFELAIAISGYLNAINPFDQPGVEAYKKNMFALLGKPGFEELGAELNARL"
BASE COUNT          595 a          509 c          430 g          640 t
ORIGIN      
        1 ttttgtaaat ttttacagta agctactggg tcgactccat acagcccact gcgtaatctc
       61 taaccaatcc ctgttgattc aagactagac catgcagaac tccgccgtaa acagctcctc
      121 cgtcaatccc cattttccca tctgaactgg tccagatttg gctaatgcgg agcttggtct
      181 ggtagaggtt ataaacaggg gtatgcccaa agacaatcat tttccctgtg gtattttcgg
      241 cctcatggaa aggggtacgt aaccaaacct tatcatgatt actagttttt cgccagtctg
      301 gcaaagtcaa gtccacacca gcatggacaa agatatatcc ttccgtttcc agatgatagg
      361 gacaagattt gacaaaatca atcaacttct catattgttc catcactgca ttagcatcca
      421 ccaaaccatc tacaggagca tccaagggac gacctaatag cgagttgatg gtcgtatctc
      481 caccattcct ccggtaatgg tcatagcgtt ccaagggatc ccgcaaccaa gccaaaaaca
      541 ttcgttcatg attcccagtc agacagatag catctttttc tcggactaat tgccagacca
      601 actccaaaca agcttttgaa ttttctccac ggtcaattaa atccccaaga aaaatcagct
      661 gttgtcgttc ttcattccat ttcttcaaaa tatcgagtag cagttcaaac tttccatgga
      721 catcgccgat tacaaaaaaa tcttttttca tagactctat tctaccatat ttaataagca
      781 aagaaaaact gaccgtttcc gatcagtttc attctattac aaacgtgcgt tcaattctgc
      841 accgagttct tcaaatcctg gttttccaag aagagcgaac atgtttttct tgtaggcttc
      901 tacacctggt tggtcaaatg ggttgatcgc attgaggtaa ccagagatag caatcgccaa
      961 ctcgaagaag tagaaggtgt aaccaagtgt gaactcatct tgttgcggaa gggtcacaaa
     1021 catgtttggt acgccaccat ctgtgtgagc aagaagcacg ccgtccgttg cttttttgtt
     1081 tacaaagtca acatccttac cttgcaagta gccaagaccg tcaaggtcct ctgccatgtc
     1141 aggaatgagg atattttttc ttggtttgtc aacacgaacc actgtctcaa agatattgcg
     1201 gtttccttct tggatgaatt gcccaagaga gtgcaagtct gttgagaagt tagcagatgt
     1261 tgggttgata cctttttggt ccttaccttc tgactcacca gccaattgtt tccaccattc
     1321 gctgaagtat tgaagtgatg gctcgtagtt agccaagatt tctgttacat agccttttct
     1381 gtaaaggatg ttacggacag ctgcatattg gtatgcttgg ttttcagcaa tcttatcaga
     1441 tgtaaatgtc gtgcgggctg cgtttgcacc ttccataagg gcatcaatgt ctgcaccagc
     1501 tgccgcgatt ggcaaaagac caactgcagt caagactgag aaacggccac cgacatcatc
     1561 tggaacaaca aaggtttccc aaccatttgc gtccgcttca accttaacag caccttttgc
     1621 cttgtcagtt gtcgcataga tacgcttgtt agcttcttct tgaccgtatt tcttgacaag
     1681 taattctttg aagacgcgga aggcaatagc aggttcagtt gttgtacctg attttgaaat
     1741 cacgttaact gagaaatcct tatcagcaac ataatccacc aagtcagcaa gatagcttga
     1801 tgagatagag tttccagcgt aaaggatttg tggagctttg cgctcttcac gtgtttgcaa
     1861 gtttacaaag gcattgctca agaaatcaat agctgcttta gcaccaaggt aagaaccacc
     1921 gataccgata actaccaaaa cttcactttc attttggatt ttagctgcag cttccttgat
     1981 acgagcaaac tcttctttgt cgtagttttc tggcaaatcc aaccagccga taaagtcgct
     2041 accaggacct gttccttgac ggagcatttg gtctgcaagg gtaacttgtg gttgcatgta
     2101 ttctacttct tgagctccaa cgaattggcc caagactttt gaataatcaa atgtaatatg
     2161 tgtcatgttt ttcc
//
LOCUS       DBNFEG010000216         2137 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.218, whole genome shotgun sequence.
ACCESSION   DBNFEG010000216 DBNFEG010000000
VERSION     DBNFEG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.218"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            97..777
                     /gene="radC"
                     /locus_tag="AC1PNG_002002"
     CDS             97..777
                     /gene="radC"
                     /locus_tag="AC1PNG_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIP6702462.1"
                     /translation="MYQIEFKEEAILPRERLVEVGAERLSNQELLAIFIRTGTKKEPV
                     SILSNKLLNRLESLAALRELSIEELQSLTGIGRVKAIEIKAMIELGKRINQSELLLNE
                     RILGSEKLGRKMIHEIGHKKQEHLVALYLNTQNQIISQKTIFIGSVNRSIAEPREILH
                     YAVKCMATSIIIVHNHPSGSVQPSRNDLLFTENLKESCEKLGLVLLDHLIVGNKDYYS
                     FREESELF"
     gene            complement(774..1466)
                     /locus_tag="AC1PNG_002003"
     CDS             complement(774..1466)
                     /locus_tag="AC1PNG_002003"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIP6702463.1"
                     /translation="MKKPVIGISGNEYKTGDHTEPLLSYTQTCLVQAIEDAGGLPLIL
                     PVTEPDLAKYYIHLIDKLILTGGQNVQPSYYREERTIDSDNYLPKRDEFELAIIRAAQ
                     ENQKPIFGICRGLQLYNVAQGGSLHQSISEHWQDIDGQEVSQTIQLTQNSPLYDIYES
                     DPSVNSFHRQAIKDLAPDLEIIALSDNQQIIEAVHSAYPTKFLGVQWHPELLYGKREI
                     EKELFHYIVNKL"
     gene            complement(1470..2108)
                     /locus_tag="AC1PNG_002004"
     CDS             complement(1470..2108)
                     /locus_tag="AC1PNG_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIP6702464.1"
                     /translation="MKNDKKSDIPRATAKRLSLYYRIFKRFYAGNIEKASSKEIAEAI
                     GIDSATVRRDFSYFGELGRRGFGYNVKELMDFFADILNDTSITNVMLVGVGNMGRALL
                     HYRFHERNKMKIVMAFEADDNPAVGTTDENIPIHAISEIKERISEANSQTAILTVPSV
                     KAQEVTDILVEAGVKGILSFSPVNLSVPKDVVVQYVDLTSELQTLLYFMRKG"
BASE COUNT          634 a          371 c          466 g          666 t
ORIGIN      
        1 gataaagaag tggtatagga ctgaggagat aatctttggt tttatatggc ttcttttttt
       61 gcaagatttt acgaatagat aggtgaggag gaaatgatgt atcaaattga attcaaagaa
      121 gaggccattc tacctagaga acgtttggta gaagttggag ctgagcgtct cagtaatcag
      181 gaactactag caatttttat tagaactggg acaaaaaaag aacctgtttc tattctctcc
      241 aataaattat tgaatcgttt ggagagttta gcggctctaa gagaactatc aattgaagaa
      301 ttgcaaagtt tgactggaat tgggcgcgtc aaggcgattg aaattaaggc tatgattgaa
      361 cttggaaaac gtatcaatca atcagagctg ctcttaaatg agagaatttt gggaagtgag
      421 aagttgggac gtaagatgat tcatgaaatc gggcataaga agcaggagca cctagttgca
      481 ctttatctca atacacagaa ccagattatc agtcaaaaaa cgatttttat tgggagtgtt
      541 aatcgtagca ttgcagaacc acgtgaaatt ttgcattacg cagtaaaatg tatggcaaca
      601 tcgattatca tcgttcacaa ccacccgtcg ggttcagtac aacctagtag gaatgatttg
      661 ctatttacgg agaatttaaa agagtcctgc gaaaagctag gactggtatt attagatcat
      721 ttaattgttg gaaataagga ttactattct tttagagaag aaagtgagtt attttaaagt
      781 ttattgacaa tatagtggaa gagttctttt tcaatctcac gtttaccgta gagtagttca
      841 ggatgccatt gaacccctaa aaatttagtt gggtaggctg aatgaactgc ttcaataatc
      901 tgttgattat ctgatagtgc aatgatttct aagtcagggg ctaagtcttt gatagcctga
      961 cggtgaaatg agttcacact cggatcggac tcatatatgt cgtaaagtgg actattttga
     1021 gtcagttgaa tcgtttgact aacttcttgt ccatctatgt cttgccaatg ttcagaaatt
     1081 gattgatgaa ggctaccacc ttgagctaca ttgtagagct gtagacctcg gcagataccg
     1141 aagataggtt tttgattttc ttgggcagcc cttatgatgg ccaattcaaa ctcgtcacgc
     1201 tttggtagat aattatcgct gtcaatagtt ctttcttcgc gatagtaact tggctggaca
     1261 ttttgtcctc ctgttaggat gagtttgtca ataagatgga tataatattt tgccaaatct
     1321 ggctctgtaa caggaaggat gagtgggagc ccgccagcat cttcaatagc ttgtacgagg
     1381 caagtttggg tataagaaag caggggttct gtatgatccc ctgttttata ttcatttcca
     1441 gaaataccaa taactggttt tttcattgct tatccttttc gcataaagta gagtagtgtc
     1501 tgtaattcac tagtcaaatc aacatactgg acaacgacat cttttgggac agacaagtta
     1561 actggtgaaa agctcagaat tcccttgaca ccagcttcaa caagaatatc ggtcacttct
     1621 tgtgccttga cgctgggtac cgttagaatg gctgtctgag agtttgcttc gctgatacgt
     1681 tccttgatct ctgagattgc atggattggg atattttcat ctgtcgttcc aacagctgga
     1741 ttatcatctg cttcaaaagc catgactatt ttcattttat ttcgttcgtg gaagcgatag
     1801 tgtagcaagg cccgccccat attcccaaca ccaacaagca taacattggt gatggaggta
     1861 tcatttagaa tatctgcaaa gaaatccatc aactctttga cattgtagcc aaagccacga
     1921 cgcccaagct ctccaaaata ggaaaaatct cggcggacgg tagccgaatc gattccgata
     1981 gcttcagcaa tttcttttga actagctttt tcaatatttc cagcatagaa acgtttaaaa
     2041 atgcgatagt agagggatag acgtttggca gtagcgcgtg gaatatcaga ttttttatcg
     2101 tttttcactt tagactcctt tatttcgata gaatacg
//
LOCUS       DBNFEG010000217         2083 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.219, whole genome shotgun sequence.
ACCESSION   DBNFEG010000217 DBNFEG010000000
VERSION     DBNFEG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.219"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..332
                     /locus_tag="AC1PNG_002005"
     CDS             <1..332
                     /locus_tag="AC1PNG_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HIP6702465.1"
                     /translation="GDFVTAVEEADAGDFVYFDPPYVPITATSSFTSYTHEGFSDQDQ
                     RRLRDTFIDLDRRGVNVMLSNSSSPVVEELYAGFNISRVDVSRTNGASNSSRGKIQEI
                     IVRNYGE"
     gene            322..1137
                     /locus_tag="AC1PNG_002006"
     CDS             322..1137
                     /locus_tag="AC1PNG_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-methyltransferase"
                     /protein_id="HIP6702466.1"
                     /translation="MANNEYKYGGVLMNKPYYHKNKAILVHADTFEFLNKMKPESMDM
                     IFADPPYFLSNGGISNSGGQVVSVDKGDWDKVDSLEEKHEFNRKWIRLAKNVLKPNGT
                     IWISGSFHNIYSVGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKDD
                     KKARHYYNYELMKELNDGKQMKDVWVGGLTKKSEKWAGKHPTQKPEYLLERIILASIQ
                     EGDYILDPFVGSGTTGVVAKRLGRKFIGIDAERDYLKIARTRLDKEDDKKNFS"
     gene            1115..2041
                     /locus_tag="AC1PNG_002007"
     CDS             1115..2041
                     /locus_tag="AC1PNG_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HIP6702467.1"
                     /translation="MTKRTFHSWFETFTDTVADWNYYVDFTKVFNNMNDLYLRTNLNI
                     LNTLVGSKQIREDFIAICDKYPDVLTVIPILLAIRLESKGRGKNRKPIALPIREYGDD
                     AIISYDFDFYSPNYAIEDYADLLENTGIFELLQSHLVKNLQDYVYGVEVGLDSNGRKN
                     RMGKIMEALVERVLQNAGLEEKTDYYKQIRTGEIESLFHIDLSSITNNGKNHKKFDFA
                     IHVNGQLYLTEVNFYSKSGSKPNETTRSYENLAEKLTEISNVHFVWITDGEGWQRSKS
                     NLEEAFERIPRLYNLHDIRNKNIKELLEMEMD"
BASE COUNT          737 a          281 c          427 g          638 t
ORIGIN      
        1 aaggtgattt tgttacggca gttgaagaag ctgatgcggg agattttgta tactttgatc
       61 ctccgtatgt tcctataacg gctactagct cttttacttc ttatacgcat gaaggttttt
      121 ctgatcagga tcagcgtcga ttaagagata ctttcataga tttagaccga agaggagtta
      181 atgtaatgct ttctaattct tcaagcccag tagttgaaga attgtacgct ggttttaata
      241 tttcccgagt agatgtatcg agaacgaatg gagcaagtaa ttccagtcgt ggaaaaattc
      301 aagaaattat tgtaagaaat tatggcgaat aacgaatata agtatggagg ggtcttaatg
      361 aacaaaccat actatcataa aaacaaggct attttggtac acgcagatac atttgaattt
      421 ttgaataaaa tgaaaccaga gagcatggat atgatttttg cagatcctcc atatttttta
      481 agtaatggag gaatttctaa ctctggaggt caagttgttt ctgtagataa gggggattgg
      541 gataaagttg attctttaga agaaaaacat gaatttaata ggaaatggat tcgcttagcc
      601 aaaaatgtgt tgaaaccaaa tgggaccatt tggatttcag ggagctttca caatatttat
      661 tcagtgggta tggccttaga acaagaaggt tttaaaatac taaataatat tacttggcag
      721 aagacaaatc ctgcaccaaa tctttcctgt cgttacttta ctcattcaac ggaaactatt
      781 ttatgggctc gaaaagatga taaaaaagct cgtcattatt ataattacga acttatgaaa
      841 gaactcaatg atggcaaaca aatgaaagat gtttgggttg gaggactaac aaaaaaatct
      901 gaaaaatggg caggtaagca tccgactcag aaaccagaat atctcttaga acgtattatt
      961 ttagcttcaa ttcaagaagg agattacata cttgatccat ttgtaggaag cgggacgaca
     1021 ggagtggttg ccaaacgttt gggaagaaaa tttattggga ttgatgctga acgggattat
     1081 ttaaaaatcg cgagaactag attggataag gaagatgaca aaaagaactt ttcatagttg
     1141 gtttgaaact tttacagata cggttgctga ttggaactac tatgttgact ttacaaaagt
     1201 ttttaataat atgaatgacc tgtatttgag aactaatttg aatatcttga atacgttggt
     1261 agggagtaaa caaattcgag aagattttat cgcaatatgt gacaagtatc cagatgtgtt
     1321 aactgtcata ccaattcttt tggctatacg tttggaatct aaaggccgag gaaaaaatcg
     1381 taaaccaatt gctttaccta ttcgcgagta tggggacgat gctataataa gctacgattt
     1441 tgatttttat tctcctaact atgctataga ggattatgcc gatttactag agaatacagg
     1501 tatttttgaa ctactgcaat cacatctagt taaaaactta caggactatg tttacggtgt
     1561 agaagtcggg ttagattcaa atggtcgtaa aaatagaatg ggtaagataa tggaagcttt
     1621 ggttgaaaga gtacttcaga atgcaggact agaagaaaaa acggactact ataaacaaat
     1681 acgaactggg gaaattgaat cactatttca tattgatcta agttctataa cgaataatgg
     1741 taaaaatcat aagaaattcg attttgcaat tcacgtaaat ggtcaattgt atttaacgga
     1801 agtaaatttc tattctaaat cagggtcaaa accaaatgaa accacacgtt catatgaaaa
     1861 tttagcagaa aaacttactg aaatttcgaa tgttcatttt gtttggataa cagatggtga
     1921 aggttggcaa aggtctaaat ctaatttaga ggaggcattt gaaagaatac caagattgta
     1981 caatctccat gatattcgga ataaaaatat taaagagtta ttagaaatgg agatggacta
     2041 atgaatttgc aagcttatat aaatttatct tctgaagaac gct
//
LOCUS       DBNFEG010000218         2029 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.220, whole genome shotgun sequence.
ACCESSION   DBNFEG010000218 DBNFEG010000000
VERSION     DBNFEG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.220"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(22..1227)
                     /locus_tag="AC1PNG_002008"
     CDS             complement(22..1227)
                     /locus_tag="AC1PNG_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIP6702468.1"
                     /translation="MAIKNQKKYKGVYCDKNGKIFYQADLGVDPVTGKRVQKKARKNQ
                     YGKPFTTMKEAYDELVRIRYEFNNNLSIENYNITFESYMNTIYLRAYKQKVQAVTYKT
                     ALPHHKLFIQYFGSKPLKGITARDCESFRLHIIEHYSENYAKNLWSRFKACMGYAERL
                     GYISSMPCKSLDNPRGKHPDTPFWTYIEFQNFIKSFDLQDYEELQRFTTIWLYYMTGV
                     RVSEGLSLWWEDVDFEHKFLKVHTTLEKNENGNWYRKDQTKTPAGERLIELDDVTISV
                     LENWRKNQVANQDTDFIISRFGEPFCKSTICRIIKRKAKEVGVPVITGKGLRHSHASY
                     LINVLQKDILYVARRMGHADKSTTLNTYSHWFNALDKTVSEEITQNVISAGLDSILCQ
                     NSCQTLSKG"
     gene            complement(1230..1478)
                     /locus_tag="AC1PNG_002009"
     CDS             complement(1230..1478)
                     /locus_tag="AC1PNG_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HIP6702469.1"
                     /translation="MKTVTYKNLMNIGFPEHTARNIIREAKRIAVKKFEEARKVDHNA
                     VQLSKSPFDNRRLGIAPKNIVEELIGISLSEDNLESEN"
     gene            complement(1748..1972)
                     /locus_tag="AC1PNG_002010"
     CDS             complement(1748..1972)
                     /locus_tag="AC1PNG_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6702470.1"
                     /translation="MYQRIRDLREDHDLTQKFVANLLSFSHTNYAKIERGEVALTADV
                     LVQLSKLYNVSTDYLLGLTDCPDRIKHKIK"
BASE COUNT          597 a          399 c          304 g          729 t
ORIGIN      
        1 gttggcggta taataggctt tttagccttt actcagagtt tggcaggaat tttggcataa
       61 aatggaatct agtcctgcac taattacatt ttgggtgatt tcttctgata ctgttttatc
      121 taatgcatta aaccagtgac tgtaggtatt caaagttgta gacttatcag catgtcccat
      181 tcgtctggcc acatataaaa tgtccttctg caaaacattt atgagatagg aagcatgact
      241 gtgccgcaat ccttttcctg ttatcactgg tactccaact tctttagctt ttctcttaat
      301 aattcgacag atcgttgatt tacaaaacgg ttcaccaaat cgtgaaataa tgaaatctgt
      361 atcttgattg gcaacttgat tttttcgcca attttccaat actgatattg tcacatcatc
      421 cagttcaatg aggcgttctc cagcaggtgt tttggtttgg tctttacgat accaatttcc
      481 attttcattc ttctctaagg ttgtatgaac tttcaagaat ttatgttcaa aatcgacatc
      541 ttcccaccat aacgataaac cttcacttac acgaacacct gtcatataat ataaccaaat
      601 agtagtgaat ctttgtaact cttcgtaatc ttgtaaatca aatgatttaa taaagttctg
      661 aaactcaata taagtccaaa atggagtatc agggtgcttg cctctagggt tatctaacga
      721 cttgcatggc atagatgaaa tatatcctaa tctctcagca tatcccatac aagccttaaa
      781 tcgtgaccat aaattctttg cataattttc agaataatgc tcaatgatgt gcaatctaaa
      841 agattcacaa tctctagctg ttatgccttt taaaggttta gagccaaaat actgaataaa
      901 taacttatga tgtggcaaag cagttttata agttacagct tgtacctttt gcttataagc
      961 tcttagataa atggtattca tataactttc aaatgttata ttataatttt caatactaag
     1021 gttattatta aactcatacc taattcgaac cagttcatca tacgcttctt tcattgtagt
     1081 aaatggctta ccatactgat tctttctagc tttcttttga actcgtttcc ctgttactgg
     1141 atcaacacca agatcagcct gataaaatat cttgccattc ttatcacaat aaacaccttt
     1201 atactttttt tgatttttta tagccatctt caattctcac tttctaaatt gtcttcagag
     1261 agtgaaattc cgattaactc ttctactata tttttaggag caataccaag tcttctattg
     1321 tcaaagggcg atttacttaa ttgtaccgca ttatggtcaa ctttgcgagc ttcttcaaac
     1381 ttttttactg ctattctttt tgcttctcta atgatgtttc ttgccgtatg ttcaggaaaa
     1441 ccaatattca tcaagttttt atatgttact gttttcatac ctccactcct gtcaaaaaaa
     1501 ttatttcctc agtaggtatg attggttaaa tcactcatgg gcatctcacc cgctgtcctt
     1561 ttacgttggc agcctgaacg ttcagaagta tcattgtcct tatttgtggt catggcagat
     1621 agagaaccac tctattttat agctaataat tatcgctcct atcacggcgt atctcactaa
     1681 attgctcgac aaataagaaa gtcccacctc ggtttattca attgtcaatg aacaaatcaa
     1741 ggagagacta ttttatttta tgttttatcc tatctggaca atctgttaac cctaataggt
     1801 aatctgtact aacgttatag agtttagata gctgcacaag aacatctgcc gttaaggcaa
     1861 cctctcctct ctcaattttt gcataatttg tatgagaaaa ggaaagtaaa tttgcaacaa
     1921 acttttgagt gagatcgtga tcttccctca aatctcttat ccgttggtac atctagcttt
     1981 ctcctaagaa aagtatatgt cactttcaga aaaaagctgg gaaattgtc
//
LOCUS       DBNFEG010000219         1961 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.221, whole genome shotgun sequence.
ACCESSION   DBNFEG010000219 DBNFEG010000000
VERSION     DBNFEG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.221"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            160..1704
                     /locus_tag="AC1PNG_002011"
     CDS             160..1704
                     /locus_tag="AC1PNG_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HIP6702471.1"
                     /translation="MSLQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGNFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLDLLQELEEVLG
                     IASYPMNWPIGMGKSFEGLYDLHNKRLELYRGDERFASLDEGDKLFGSNPFYAQVLDD
                     IELLAEAGNEFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTTGD
                     VIDPLNKDFSGFVFKIQANMDPRHRDRIAFVRVVSGEFERGMAVNLPRTGKGAKLSNV
                     TQFMAESRENVENAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
BASE COUNT          552 a          424 c          485 g          500 t
ORIGIN      
        1 tctgtagacc tattcttcaa tagtttgaaa aattccctta tttccgatat aataagagag
       61 ttgtctagta aagaataagg atgaatccag tgtaaatcga ataggataag agctcagctc
      121 tgctatgaat caaaatacaa agaatgaaaa ggaaacatta tgtcactaca agaagaaatc
      181 aagaaacgcc gcacctttgc gattatctct cacccggacg cggggaagac cactattact
      241 gagcagttgc tctactttgg tggtgaaatt cgtgaagcag gtacggtcaa ggggaaaaag
      301 actggtaact ttgccaaatc tgactggatg gatattgaga agcaacgtgg tatctctgtt
      361 acctcatctg tcatgcagtt tgactatgca ggcaagcggg tcaatatcct agatacacca
      421 gggcacgagg acttctcaga agacacttac cgtaccttga tggcggtgga tgcggctgtc
      481 atggtggtgg actctgccaa gggtatcgag gcccagacca aaaagctctt tgaggttgtt
      541 aagcaccgca acattcctgt tttcactttt atcaacaagt tggaccgtga cggtcgtgag
      601 ccacttgatt tattgcagga gttggaagaa gtcttgggca ttgccagcta tccaatgaac
      661 tggccgattg gtatggggaa atccttcgag ggactctatg acctccataa caaacgttta
      721 gaactctatc gaggtgatga gcgttttgcc agcttggacg aaggcgacaa gctctttggt
      781 tctaatccct tttatgcaca ggttttggat gatattgaac ttctggcgga agctgggaat
      841 gaattttcag aacaggcgat tttggacggc gatttgacac ctgttttctt cggctcagcc
      901 ctgacaaact ttggtgtgca gaccttcctt gacaccttct tggaatttgc tccagagcca
      961 catggtcaca agacaacgac aggggatgtc attgacccgc tcaataaaga tttttcaggc
     1021 tttgtcttca aaatccaagc caacatggac cctcgtcacc gcgaccgtat cgcctttgtc
     1081 cgtgtggttt cgggcgaatt tgaacgcggt atggcggtta acctgcctcg tacaggtaag
     1141 ggagccaagt tgtccaacgt gacgcagttc atggccgagt cccgtgagaa tgtggaaaat
     1201 gcagtagcgg gtgacattat cggggtttat gatacaggga cctatcaggt aggagatacc
     1261 ttgaccgtcg gaaagaacaa atttgagttc gaaccgctac cgaccttcac ccctgaaatt
     1321 ttcatgaaag tttctgctaa aaacgtcatg aaacaaaaat ccttccacaa gggaatcgag
     1381 caactggtac aggaaggggc tatccagctc tataagaact accagactgg cgagtatatg
     1441 ctgggtgccg ttggtcagct ccagtttgaa gttttcaaac accgcatgga aggcgagtac
     1501 aatgccgaag tagtcatgac tccaatgggt aaaaagacag tccgctggat caagccagaa
     1561 gacctggatg aacgcatgtc atcaagccgc aacatcctag ccaaagaccg ctttgaccaa
     1621 ccagttttcc tttttgaaaa cgacttcgcc ctccgctggt tcgcggacaa atatccggat
     1681 gtggagttgg aagagaagat gtagcggaaa gacagaaatc agtaatcaac aaagttgatt
     1741 tgatttcgtc gtctttccct cgcaagggcg tccaaagaac tcgagcaatg cgaagagtga
     1801 tttgctgacg cctactgcgt cgaggttcag tgaacctttt ctagtgggag atgtgaactg
     1861 acggtagtca gttcacattg ataaagataa ctataaaagg atatgataga atacatgaag
     1921 ctgggcaaga cctctatgat gtcttgccca gcttcatttt t
//
LOCUS       DBNFEG010000220         1931 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.222, whole genome shotgun sequence.
ACCESSION   DBNFEG010000220 DBNFEG010000000
VERSION     DBNFEG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.222"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(89..241)
                     /locus_tag="AC1PNG_002012"
     CDS             complement(89..241)
                     /locus_tag="AC1PNG_002012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702472.1"
                     /translation="MSKWYLERKDAEFKTKTKLEHDGMVSVAPHNPFEDLTVEELRAI
                     IAEDAG"
     gene            complement(277..480)
                     /locus_tag="AC1PNG_002013"
     CDS             complement(277..480)
                     /locus_tag="AC1PNG_002013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702473.1"
                     /translation="MMKNKGGRPTKMTQGTVKKLEEAFLRGLSDEEACLYANQPCMII
                     ARKTHSLLTEKNYLSNVLKHELN"
     gene            complement(530..1078)
                     /locus_tag="AC1PNG_002014"
     CDS             complement(530..1078)
                     /locus_tag="AC1PNG_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702474.1"
                     /translation="MTLNTFSDTANTFTFNYTFKDHDTAQVAGHALMGYMTGTFEQPG
                     IEVYYDNDKVGGDYNRLAVEYVADTELTETFRRICDSFQGYYNDPEAETDVEDQYRLE
                     RVEQLKQSETFDSLLEKVVIYELELLDYAERLLSDDPIPTDTEMAYMTLNLIGGKGVG
                     LFKSLDEDNEYSGLAYYNAEAE"
     gene            complement(1156..1614)
                     /locus_tag="AC1PNG_002015"
     CDS             complement(1156..1614)
                     /locus_tag="AC1PNG_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702475.1"
                     /translation="MKEQEFFEQAEKELEELNQHRADFMTMDFKELNNADYINFLNIG
                     NRIFSEDTTLNVYELYKHPDTRAKCFATIAKIAYHVNNMFQTEERMRTMIDSLELHFQ
                     NMVKKLVHQTDSDKLAELLLEIKKDNPNMTAEQESQFIRDIAVSGLLAMQ"
     gene            complement(1627..>1931)
                     /locus_tag="AC1PNG_002016"
     CDS             complement(1627..>1931)
                     /locus_tag="AC1PNG_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HIP6702476.1"
                     /translation="IYDYYQQRIGSLDGYQYEKLKDYLDIDKLEPELVKRAIDRGADN
                     SKRNFGYINKILKNWAQNGIRTIAQQDEEQRQFVDSKANKQKSNDWVPDPNYPPPY"
BASE COUNT          452 a          438 c          340 g          701 t
ORIGIN      
        1 taatcacgcg cttccatacg ttcgtctatt cgtcgtttta gaaattgccg aagtacttca
       61 cctagataac cctctcaacc aatagtattt atcccgcatc ttcagcaatg attgctcgta
      121 actcttcaac cgtcaaatct tcaaatggat tatgaggcgc aacggatacc ataccatcat
      181 gttcaagttt tgtcttagtc ttaaattcgg catctttccg ttctaggtac cattttgaca
      241 agtccacatt cccgtcttca atcgcttttg atatatttag tttagctcgt gttttaacac
      301 gttgcttaag tagttctttt cggtcagtaa actgtgggtt tttcttgcaa taatcataca
      361 gggttggttt gcatacaaac aagcttcttc atcgcttagc cctcttagaa atgcttcctc
      421 taatttcttt accgttcctt gtgtcatttt tgtaggtcta ccgcctttat ttttcatcat
      481 gttattcctc tctaatttta gcacaaaaag aggcggttgc ctctctctgc tattctgcct
      541 cagcgttgta ataagcaagc ccgctgtatt cgttatcttc gtccagtgac ttgaatagcc
      601 ctacaccttt accgccaatt aagtttagtg tcatataagc catttccgta tctgtcggaa
      661 tagggtcatc acttagcaaa cgttccgcat agtccagcaa ctccagctca taaatcacca
      721 ccttttcaag caagctatca aaagtttctg actgcttgag ttgttccacg cgctccaggc
      781 ggtactggtc ttcaacgtcg gtttctgctt caggatcgtt gtagtagcct tggaaactgt
      841 cacaaatccg cctgaaggtt tcagtaagtt cagtatctgc cacatattcc accgccaaac
      901 ggttgtaatc tccgcccact ttatcattat cataatacac ttcaatccca ggttgttcaa
      961 atgttcctgt catgtagccc ataagcgcgt gccctgcaac ttgtgcggtg tcgtggtctt
     1021 tgaatgtgta gttaaatgta aatgtgtttg ctgtgtctga aaatgtgttt aatgtcatgt
     1081 ctttattctc cctagttgtt tgataaattt ggaaaagttt ccttatttcg gtatcatcgg
     1141 tcacggtgtc gcctcctact gcattgctaa tagtccacta actgcaatat ctcgtataaa
     1201 ctggctttcc tgttctgctg tcatattcgg attgtctttc ttgatttcca gtagtagctc
     1261 agccagcttg tcactatctg tctgatgtac cagtttctta accatgtttt gaaaatgcag
     1321 ttcaagacta tcaatcatgg tacgcatacg ttcttctgtc tgaaacatgt tgttaacgtg
     1381 atatgcaatt ttggcaatgg tcgcaaagca ttttgctctc gtgtctggat gcttatatag
     1441 ttcgtacacg ttcaaagtgg tatcttcgga aaaaatccgg tttccaatgt tcaaaaagtt
     1501 tatatagtct gcattattga gttctttaaa atccatagtc atgaagtcgg ctctgtgctg
     1561 gtttaattcc tctaactcct tttctgcttg ctcaaaaaat tcttgttctt tcatttgcgc
     1621 ctctttctag tatggtggtg gatagttcgg gtctggcacc caatcgtttg atttttgttt
     1681 atttgcttta ctatcgacaa actgtctctg ctcctcgtct tgctgagcta ttgtccgaat
     1741 gccattctgt gcccaatttt ttagaatctt attgatgtag ccaaagttcc tttttgagtt
     1801 gtcagctcct ctatcaattg ccctcttgac aagttcaggt tcaagcttat caatatctaa
     1861 gtaatctttt agtttctcat actgataacc gtccaaggaa ccaattcttt gttgataata
     1921 atcataaata t
//
LOCUS       DBNFEG010000221         1858 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.223, whole genome shotgun sequence.
ACCESSION   DBNFEG010000221 DBNFEG010000000
VERSION     DBNFEG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.223"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..531)
                     /locus_tag="AC1PNG_002017"
     CDS             complement(<1..531)
                     /locus_tag="AC1PNG_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIP6702477.1"
                     /translation="MKERIKDFISVTLGSIVMAIGFNSFFLENNIVSGGVGGLAIALN
                     ALLGWSPSDFVLYCNIPLLIICWFFLGKSVFIKTVYGAIIYPLCIKLTAGLPNLTENP
                     LLAAIFGGIILGFGLGLVFLGNSSTGGTGILIQFIHKYTPLSLGLTMAIIDGIIVGLG
                     FVAFDTDTVMYSIIALM"
     misc_binding    633..839
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            886..>1858
                     /gene="trpS"
                     /locus_tag="AC1PNG_002018"
     CDS             886..>1858
                     /gene="trpS"
                     /locus_tag="AC1PNG_002018"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIP6702478.1"
                     /translation="MSKPIILTGDRPTGKLHIGHYVGSLKNRVLLQDAGQHELFVFLA
                     DQQALTDHAKEPQKIVESVGNVALDYLAAGLDPAKTTIFIQSQIPELAELTMYYMNLV
                     SVARLERNPTVKTEIAQKGFGEGIPAGFLVYPVSQAADITAFKANFVPVGNDQKPMIE
                     QTREIVRSFNHAYQTDILVEPEGIYPENEAAGRLPGLDGNAKMSKSLGNGIYLADDMD
                     TLKKKVMSMYTDPNHIRVEDPGKIEGNMVFHYLDVFGREEDKAEIEAMKEHYQRGGLG
                     DVKTKRYLLDILERELGPIRERRIEFAQDMGQVYAMLEAGSQKARQVA"
BASE COUNT          593 a          338 c          425 g          502 t
ORIGIN      
        1 cattagggca ataatagagt acataacagt atcagtatcg aaggcaacaa atccaagacc
       61 aacgataata ccatcaataa tagccattgt cagacctaat gataaagggg tgtacttgtg
      121 aataaattga ataagaatac ctgttccacc agtagaagaa tttccaagga agacaagccc
      181 aagtccaaaa cctaggataa ttccaccgaa aatagcagct aggagaggat tttctgtcaa
      241 gttgggtaaa cctgctgtca acttgatgca aagagggtag ataatagctc catagacagt
      301 tttgataaat acagattttc ctaagaaaaa ccaacagatg attaataaag ggatgttaca
      361 gtagaggaca aaatcagacg gactccaccc cagaagagca ttgagtgcga ttgctaaacc
      421 tccgacacca ccagatacga tattattctc tagaaaaaaa ctgttaaatc caatggccat
      481 gacaattgat ccgagtgtga cagagataaa gtcctttata cgttctttca tatattctcc
      541 ttggttagtg ttataaaagc aatttctatt atccctaatt tagacagtat cgtcaaggct
      601 ttgtcagctt gactttctaa ttttttgcgg ttataatagg aatcaatcag aagagtagta
      661 agtggataat ttttagagag tttgtggttg gtgtgaacaa acaagagtcg cttatgaatg
      721 gactgagtga atagaatctg aataaataag gattcggcta gcaggcctta tgtgcaaaaa
      781 gatgtaagct tatttaatgc ttataagtga ggtggcaccg tgtccttgac gccctcgcac
      841 agttttctgt gcgtgggctt ttattatgta aaaggagaac attttatgag taaaccgatt
      901 atcctaacag gagaccgtcc aacaggcaaa ttacatattg gacattatgt tggttcgttg
      961 aaaaatcgtg ttttattaca ggatgcaggt caacatgaac tttttgtctt tttagcagac
     1021 caacaagcgt tgacagatca tgcaaaggaa ccgcaaaaga ttgttgaatc cgttggaaac
     1081 gtagcactag attatctagc ggcaggactt gatccagcta agacaacgat tttcatccag
     1141 agtcaaattc ctgaattggc agagttgacc atgtactaca tgaacttggt atctgttgca
     1201 cgtctagaac gcaatccgac tgtaaaaacc gagattgctc agaagggatt tggcgaagga
     1261 attccagctg gtttcttggt ctatcctgtg tcacaagcgg cggatattac agccttcaag
     1321 gcaaactttg ttccagtagg aaatgaccag aaacctatga ttgagcagac tcgtgaaatc
     1381 gttcgtagct ttaaccatgc ttaccagact gatattttgg tagagccgga gggcatttat
     1441 cctgaaaatg aggcagctgg tcgcttgcca ggtttggacg gcaatgccaa gatgtctaag
     1501 tcacttggaa atggtattta tctggcagat gacatggata cactcaagaa gaaagtcatg
     1561 tccatgtaca ctgatcccaa ccacatccgt gttgaagacc caggtaagat tgaaggaaac
     1621 atggttttcc actatcttga cgtttttggt cgcgaagaag ataaggctga gattgaggct
     1681 atgaaggagc actatcaacg tggtggtttg ggcgatgtga agaccaaacg ttatttgctg
     1741 gacattttag agagagaatt aggaccaatc cgtgagcgtc gtatcgaatt tgcacaggat
     1801 atgggacagg tctatgcaat gttggaggca ggtagccaaa aagctcgcca agttgctg
//
LOCUS       DBNFEG010000222         1819 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.224, whole genome shotgun sequence.
ACCESSION   DBNFEG010000222 DBNFEG010000000
VERSION     DBNFEG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.224"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(86..1291)
                     /gene="rpsA"
                     /locus_tag="AC1PNG_002019"
     CDS             complement(86..1291)
                     /gene="rpsA"
                     /locus_tag="AC1PNG_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIP6702479.1"
                     /translation="MNEFEDLLNSVSEVTPGDVVTAEVLTVDAGQANVAISGTGVEGV
                     LTLRELTNDRDADINDLVKVGETLELLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKVTRAVKGGLAVEFEGLRGFIPASMIDSRFTRNTERFVGQEFDAKIKE
                     VDPAENRFILSRRDVVEAEAAAARAEVFGKLAVGEVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSAVTVGEEVEVKVLAIDEVEGRVSLSLKATQPGPWDGVEQKL
                     AAGDVIEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDALSVGQEVTVKVL
                     EVNAADERVSLSIKALEERPAAVEGEEKAEKRAPRPRRQKREEKRDYELPETQSGFSM
                     ADLFGDIEL"
     gene            complement(1415..1486)
                     /locus_tag="AC1PNG_002020"
     tRNA            complement(1415..1486)
                     /locus_tag="AC1PNG_002020"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1452..1454),aa:Gln,seq:ttg)
BASE COUNT          551 a          435 c          314 g          519 t
ORIGIN      
        1 catctcaaga accggaatct tacgtgatat ccattacact aagggtggaa aggaaaaact
       61 tgcacaaggc aagtttttat atgaattaca attcaatgtc accgaaaagg tcagccattg
      121 agaatccaga ttgagtttct ggcaattcgt aatcacgttt ttcttcacgt ttttgacgac
      181 gtggacgtgg agcacgtttt tcagcttttt cttcaccttc aactgctgct ggacgctctt
      241 ccaaagcttt gatagaaagt gatacacgct catcagctgc gttcacttca agaactttaa
      301 cagttacttc ttgacctact gacaaagcgt cttttggatt ttcaacacgt ttgtgtgaaa
      361 tttgtgaaat gtgtaccaaa ccatcgatac ctggcaatac ttcaacgaaa gcaccgaagt
      421 cagtcaagcg ttttacttta ccttcgatta catcaccagc tgccaatttt tgctcaacgc
      481 catcccatgg accaggctgt gtagctttca atgacaatga tacgcgacct tcaacttcat
      541 cgattgcaag aactttaact tcaacttctt caccaactgt tacagctgat ttaggagata
      601 cgttacgctc gtgagacaat tcagtcaagt gtaccaaacc atctacacca ccaaggtcga
      661 tgaatgcacc gaagcttgtg atacgtgcaa ctttaccagt tacaacttca ccaactgcca
      721 atttaccgaa tacttccgca cgtgcagctg cagcttctgc ttcaacaacg tcacgacgtg
      781 acaagatgaa acggttctca gcaggatcaa cttctttgat tttagcatca aattcttgac
      841 ctacaaaacg ttcagtgtta cgagtgaaac ggctgtcgat cattgaagct gggatgaaac
      901 cacgaagacc ttcaaattca actgcaagac cacctttaac ggcacgagtt actttaactg
      961 taacaacttc ttcttcacga ccaacaagct tgtcccatgc tttacgtgct tccaaacgtt
     1021 ttttagatac aagataagta acagtgtctg tatctttacc aacaacttgg cgaagtacta
     1081 acaattcaag tgtttcacca acttttacaa ggtcgttgat atcagcatca cggtcgttag
     1141 tcaactcacg aagagtcaat acgccttcaa caccagtacc tgagatagct acgtttgctt
     1201 gaccagcatc aactgtcaat acttctgcag ttaccacatc accaggtgta acttcgctta
     1261 cactgttcaa caaatcttca aattcgttca ttataaaaaa acctccgaca atcaggtctt
     1321 tcgacccaac ataaaatata ttttatttaa ggcacacgca aggacaacaa caattagaca
     1381 tctttgacga actagactat atctagtacc tttactgggg tagctggatt cgaaccaacg
     1441 catgagggag tcaaagtccc ttgccttacc gcttggcgat accccaaaaa atagatacaa
     1501 ttcatttatt tactaatcac aagaaattga actcgagatt aatttcttag gaaaatagat
     1561 agccttcctt gagtgttaac attcagcgtc agtctcctaa ttttctacag aaatttctaa
     1621 taaaccagaa catctctcgt atcttagttg aacacggact aaaatcctag taaaaaagat
     1681 aaacttcctt gtgtgtttag cacactacgt cagtttccta ttttcatacg gatttcttaa
     1741 cgtccattgt atcctgttca atggagagag agggattcga acccccgaac ccgaaggagc
     1801 ggatttacag tccgccgcg
//
LOCUS       DBNFEG010000223         1812 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.225, whole genome shotgun sequence.
ACCESSION   DBNFEG010000223 DBNFEG010000000
VERSION     DBNFEG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.225"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..585
                     /locus_tag="AC1PNG_002021"
     CDS             <1..585
                     /locus_tag="AC1PNG_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HIP6702480.1"
                     /translation="YWFHHGHLGLTKADMLYPRKGKRVKKQASPNFKPAGKSIEERPD
                     VINLRLENGHYQIDTVLLTRGKNHCLLVLTDRRSRHQIIRLIPNKTAESVNQALEGIL
                     REHRILSITVDNGTEFSRLAEVLPEEYIYYAHPYSSWERGTNENHNRLIRRWLPKGTK
                     KTTPKEVAFIEHWINNYPKKCLDYKSPSEFLMGG"
     gene            complement(944..1812)
                     /locus_tag="AC1PNG_002022"
                     /pseudo
     CDS             complement(944..1812)
                     /locus_tag="AC1PNG_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730292.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          561 a          298 c          364 g          589 t
ORIGIN      
        1 tattggtttc atcatggcca tttaggattg acgaaagcag atatgcttta ccctagaaaa
       61 gggaaaaggg tcaagaagca agctagtcct aactttaaac cagcaggaaa atctatcgaa
      121 gaacgtcctg acgttattaa tcttcgcttg gaaaacggtc attatcaaat tgatacagtt
      181 ctactaacca gagggaaaaa tcattgcctc ttagtcttaa ccgaccgacg aagcaggcac
      241 caaatcatac gattaattcc aaacaaaacc gccgaatcgg tcaatcaggc actggaggga
      301 atcttgaggg aacatcgtat cctgtctata actgtagata atggtacaga atttagtcgt
      361 ctggctgaag tgttacctga ggagtatatc tactatgcgc atccctactc ttcatgggag
      421 aggggaacga atgaaaatca caatcgattg attcggagat ggttacctaa aggaaccaag
      481 aaaacgactc caaaagaagt cgcttttatc gaacattgga tcaacaacta ccctaaaaaa
      541 tgcttggact acaagtcgcc gagtgaattt cttatgggtg gctaatttca acttgaaatt
      601 tggggtctaa gctacttata acatttgaaa ccttaattta taagtttgag ttgaggattg
      661 tggatttcaa gcagctgttt tcttctaaaa aaataacaaa atcgaaacgg tccatatcca
      721 ctttgagtgg tagttggcta tttttgattt taaatgagta tcacacccca tcgtcccatt
      781 gactgttccc caatctcgcc gctttgagaa gaaaggcaga aatgatattc tgatgaaaat
      841 tcgtctcgga aaagtggaag tcacattctt tcactctatc aaccaagaaa cattagaaac
      901 aatcttagat aaggtactat tttatgacca tccgactcag tgattaggtc aagtctactt
      961 ggtttgcggg aagtagtggt gtgatttggt taagaggatt atgctcactt agaatttcta
     1021 cctgcttgct ttgagagaca tgagtgtaga tttgtgttac attgatgtta ctgtggccca
     1081 gtaaatgttg tatttgtcga atatcgacgc cattatcaag caacatagtt gcaaaactat
     1141 gtcgaaacat atgtggagtg atatgttttt tcagttgtag cgagtgagaa atatttttca
     1201 gacttaagcg cacactttgt tctttaagat gatcatcgtt tttcacactg gtaaatagat
     1261 aactagaatg actacaatga tgcgctatat agtcttcaag taacgaaagt gtttcatcgt
     1321 ttccaatata gagtagtcgc actttctttc ctttttcgag tatttgaatg gttctatttt
     1381 ccaaattgat attatcaagt tttaaattac ataattcaga gatgcgtaga ccagttgata
     1441 aaagtagcga gataaggagt agatttcgca tagctttccg cttagaatgc ttatttttag
     1501 aattttctat ttgttggtgg gcgaaacaat atattttttg taaatctttc aaggaaatcg
     1561 ttttaggaag ctttttctct tccttgtagt ggagtcgaag ctgatggaag ggattttttg
     1621 aaattagttt ttcttcttcc agaaatttat agtatgattt aagactagca atcttacgtt
     1681 ttaatgtgct agtctttttt atatgttggt tgagaaaagt aagataggac ctaatatcat
     1741 tggtcctaaa gctataatac taatataaat tggatttgta tgcttggata gtatgagaac
     1801 taagtcgtct tt
//
LOCUS       DBNFEG010000224         1811 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.226, whole genome shotgun sequence.
ACCESSION   DBNFEG010000224 DBNFEG010000000
VERSION     DBNFEG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.226"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            207..1031
                     /locus_tag="AC1PNG_002023"
     CDS             207..1031
                     /locus_tag="AC1PNG_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6702481.1"
                     /translation="MITVQHIHKQFGDKVALDNISFDIQEGQIFGFLGPSGSGKTTMI
                     KIITGQLPADSGKSYLLGKESTLLSPEDFEAMGLVSDSSGFFEKMTLENNLLFYAKFF
                     DVSPKEVEVILQKVGLLDSKKTIAEKLSTGMKQRMLLARALINSPKVLFLDEPTSGLD
                     PTTSKRIHELLLELKGKGTTIFLTTHDMAEATFLCDQLVLLNQGNLVEKGSPQDLIHK
                     YNVTKKVRVTFEDGSDTELLFSELTPEKLAQATTIHSCEPTLEDIFIQQTGATLNV"
     gene            1024..1758
                     /locus_tag="AC1PNG_002024"
     CDS             1024..1758
                     /locus_tag="AC1PNG_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6702482.1"
                     /translation="MYKRFLALLWLRTQVLVTNKNILLPVLMPYFMLLLFKFVMKTDD
                     TKAMDLMGICFSMAIGMSVGSPISAMISEEKEKNNLSTLLLTGVRPSEYVLSILCYPV
                     VISFVNLILFPAITQADVSGYMLSYSVIMLLTSLASILINLLIGATSQSQSKSQVTSM
                     MITMAVSMGPSLSLMNETVGQILKYSFLGAYIEFFKNPHFELLNQEFYYLLGWIVLIS
                     LALILYLGNWMKYFKNSLSGIKEKIQ"
BASE COUNT          577 a          299 c          382 g          553 t
ORIGIN      
        1 atgcagacga tttgcgaata agtaagtgag agggaaaatt cccctcaagg agatacacac
       61 ttgaacaaaa taaatcattt tcaaaatgat ttacgaataa acaagtgtag gaaaaatgaa
      121 aggagaagaa ggatgattat agcaccagct ttatggattt tatcatggtg gaaaaaataa
      181 gtttgtttta atttagggga gtagagatga taacagttca acatatccat aaacagtttg
      241 gagacaaggt tgctttagac aacattagct ttgatattca agaaggacaa atctttggct
      301 tcttgggtcc ctctggatcg ggaaagacaa ccatgattaa gattattacc ggccagctac
      361 cagcagatag cggaaaatcc tatctactag gtaaagaatc gaccctattg tcaccagagg
      421 actttgaagc aatgggcttg gtcagtgatt cgagtggttt cttcgagaag atgaccttgg
      481 aaaataattt gttgttctat gcaaaattct ttgatgtcag tccgaaagaa gtagaggtta
      541 ttctgcaaaa agtagggtta ctggatagta aaaagacaat tgcagaaaaa ctatctacag
      601 gtatgaaaca gcgaatgttg cttgccagag ccctgatcaa ttcaccaaaa gtattgtttc
      661 ttgatgagcc gacaagtggt ctagatccaa caacttcaaa acggattcat gaactattat
      721 tggagctaaa aggtaagggg acgacgatct tcttgaccac gcatgatatg gcagaagcga
      781 cattcttatg tgatcaattg gttctattga atcaaggaaa tttggttgaa aagggaagtc
      841 cgcaggattt aattcataag tacaatgtca ctaaaaaagt gagggttact tttgaggatg
      901 gtagtgatac tgagctgttg tttagtgagt tgacacctga gaagttagct caggcaacca
      961 ctattcactc ttgtgagccg actttggaag acatttttat tcaacagaca ggagcaacct
     1021 taaatgtata aacgattttt agccttgctt tggcttcgca cacaagtctt ggtgaccaat
     1081 aaaaacatcc tattgccagt attgatgcct tacttcatgt tattgctgtt taagtttgtc
     1141 atgaaaacag atgataccaa ggcaatggat ttgatgggaa tttgtttttc aatggctatt
     1201 ggtatgtcag taggaagtcc tatatctgca atgatttctg aagaaaagga aaagaataat
     1261 ctgtctacct tgttactgac aggtgttcgc ccaagtgaat atgtgctgtc tattttgtgt
     1321 tatcctgttg tcattagctt tgtcaacctc attctatttc cagctatcac acaggccgat
     1381 gtatcaggat acatgcttag ctactcagtc atcatgctct tgacatcatt ggcttctatt
     1441 ctaatcaacc ttttaatcgg ggcaacttcc caatctcaat ccaagtcaca ggtgacctcg
     1501 atgatgatca caatggcagt atctatggga cctagcctgt ctttgatgaa tgagacagtg
     1561 gggcagattt tgaaatatag ttttctagga gcttatattg aatttttcaa aaatccccat
     1621 tttgaacttt tgaatcaaga attctattac ctattggggt ggattgtcct gattagtttg
     1681 gccttgattc tctacttggg aaattggatg aaatatttta agaactccct atcaggaata
     1741 aaagaaaaaa tccaatagtc tttataatat gagtaaatta gaaaggagaa atttatggta
     1801 tcagcattca t
//
LOCUS       DBNFEG010000225         1773 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.227, whole genome shotgun sequence.
ACCESSION   DBNFEG010000225 DBNFEG010000000
VERSION     DBNFEG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.227"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1060
                     /gene="dnaN"
                     /locus_tag="AC1PNG_002025"
     CDS             <1..1060
                     /gene="dnaN"
                     /locus_tag="AC1PNG_002025"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIP6702483.1"
                     /translation="AIPILSTVKITVTSEGITLTGSNGQISIEHFISIQDENAGLLIS
                     SPGSILLEAGFFINVVSSMPDLVLDFNEIEQKQIVLTSGKSEITLKGKEAEQYPRLQE
                     VPTSKPLVLETKVLKQTINETAFAASTQESRPILTGVHFVLTENKNLKTVATDSHRMS
                     QRKLVLDTSGDDFNVVIPSRSLREFTAVFTDDIETVEVFFSNNQILFRSEHISFYTRL
                     LEGTYPDTDRLIPTEFKTTAIFDTANLRHSMERARLLSNATQNGTVKLEIANNVVSAH
                     VNSPEVGRVNEELDTVEVSGEDLVISFNPTYLIEALKATTSEQVKISFISSVRPFTLI
                     PNNEGEDFIQLVTPVRTN"
     gene            1304..>1773
                     /locus_tag="AC1PNG_002026"
     CDS             1304..>1773
                     /locus_tag="AC1PNG_002026"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIP6702484.1"
                     /translation="MTLYILANPNAGSHTAEHIIFQIKESYPQLAVNIFMTVGPEDEK
                     SQIEAILKEFVSSEDQLMILGGDGTLSKALRFWPASLPFAYYPTGSGNDFAKAMNITS
                     LYRSVDAILEGKTSRIYVLNSSYGTVVNSMDFGFAAQVINGSTNSILKKNSEQG"
BASE COUNT          572 a          314 c          331 g          556 t
ORIGIN      
        1 tgctattcca attctttcaa cagtaaaaat tacagtaact agtgaaggaa tcactttaac
       61 tggttcaaat ggacaaatct cgattgaaca ttttatttct attcaagatg aaaatgcagg
      121 acttttgatc agttctccag gctccattct cttagaagct ggtttcttta ttaatgtcgt
      181 atccagcatg ccggatttag tccttgactt caatgaaatt gaacaaaagc aaatcgtttt
      241 gacaagtggt aagtctgaaa tcacattaaa gggaaaagaa gcagaacagt atcctcgttt
      301 acaggaagtt ccaacttcaa aaccattggt gttagaaacc aaagtattaa aacaaacaat
      361 taatgaaaca gcatttgcag cttctacaca agaaagtcgt cctattctta cgggtgttca
      421 ttttgtttta acagaaaata aaaatctaaa aactgttgca acagattcac accgtatgag
      481 ccaacggaaa ttggtccttg atacctctgg tgatgatttt aatgttgtca ttccaagtcg
      541 ttctctccgt gaatttactg cagtttttac agatgatatt gaaacagtag aagtcttctt
      601 ttcaaataat caaatccttt ttagaagcga gcatattagc ttctatacac gcttattaga
      661 aggtacctac cctgataccg accgcttaat tccaactgag tttaaaacaa ctgcaatttt
      721 tgatactgca aatcttcgtc actcgatgga gcgtgctcgt cttctttcaa atgcaaccca
      781 aaatggtaca gtaaaactag aaattgctaa taatgttgta tcggctcatg taaattctcc
      841 agaagttgga cgtgtgaatg aggaattaga tactgtagaa gtatcaggtg aagatttagt
      901 aatcagcttt aacccaactt acttgataga agcattgaaa gccacaacta gtgaacaagt
      961 gaaaattagc tttatctctt ctgtccgtcc atttacattg attccaaata atgaagggga
     1021 agattttatt caattggtta caccagttcg taccaactaa atagtattaa ggaggctggg
     1081 caaaaagacc aacctcgctt ctcagggttc gtgtcaacat ctcagcgcag tggttgattg
     1141 gcagatttgt tcgtgtttta cactccaaat ctgacctaat caactgtgcg ggggcgggaa
     1201 gacgaactct ttttttgacc agtcgagttc tttcccgctc cctttatttt atactaaaaa
     1261 atagcaccta gcttattttt atatatttag tgatggggaa taaatgacgt tatatatatt
     1321 agctaatcct aatgctggta gccatactgc tgaacatatc atattccaaa taaaagaaag
     1381 ttatccacag cttgcagtta acatttttat gacagttggt cctgaggatg aaaaaagtca
     1441 aatagaggct attttaaagg agtttgtcag tagtgaggac caattaatga ttttaggtgg
     1501 agacggcaca ctgtctaaag ctttgcgttt ttggccagct agtctaccgt ttgcttatta
     1561 tccaacagga tctggaaatg attttgctaa ggcaatgaat ataacatcgc tctatagaag
     1621 tgtagatgcc attttagagg gaaaaacaag tcggatatat gttttaaata gttcatacgg
     1681 aacggttgta aacagtatgg attttggctt tgcagctcaa gttatcaatg gttcaacgaa
     1741 ttcaattttg aaaaaaaatt ctgaacaagg taa
//
LOCUS       DBNFEG010000226         1727 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.228, whole genome shotgun sequence.
ACCESSION   DBNFEG010000226 DBNFEG010000000
VERSION     DBNFEG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.228"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            169..1686
                     /gene="malQ"
                     /locus_tag="AC1PNG_002027"
     CDS             169..1686
                     /gene="malQ"
                     /locus_tag="AC1PNG_002027"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIP6702485.1"
                     /translation="MKVRQSGVLMHISSLPGKYGIGSMGQSAYEFVDFLVRTRQRYWQ
                     ILPLGTTSYGDSPYQSFSAFAGNTHFIDFDILIDKGWLAAEDLHGVDFGQNPTEVDYA
                     AIFYARRPLLEKVVVNMKAQGLPEDYWMFLGENDFWIHTFAEYMAIKEHFDLKPWTEW
                     EDQGARLRYHDTLEYYRHLLADRIDYHRITQYLFFSQWKALKAYANAKGVEFVGDMPI
                     YIAADSADMWANPHFFKTDEEGKPSVVAGCPPDAFSEIGQLWGNPIYDWEAMKHDGFT
                     WWVARLRESFKIYDMVRIDHFIGFASFWEIPAGEETAVNGYRVEAPGFELFDTIKREL
                     GDLNIIAEDLGSVTDKVIQLRDTTGFPGMKVLQFAFDPEGDSIEMPHNHPNNVVAYTG
                     THDNDTILGWYENETTKESRAFLDKYSNRREGETVSHALFRLLFGSPAFMAVATMQDL
                     LGLDGSARMNLPNTLGGNWTWRMTADQLTPEIEAELLDLTETYRRVNVHLVDEKS"
BASE COUNT          495 a          301 c          417 g          514 t
ORIGIN      
        1 tttaaaaaaa tataagaatt tttttgcaca tatttaatga aaagaaatat ggaatggcat
       61 gtgaactaac agaattaaat ggaaattcct tgatttgtta ccgttttcag tatataataa
      121 aaaagagaaa acgtttgcgt tgtgcaagcg gattagatag gagaacttat gaaagtaaga
      181 cagagtggtg tgctgatgca catttcatca ttacctggga aatatggaat tggttccatg
      241 ggacaatcgg cttatgaatt tgttgatttt ttggtaagaa ctaggcaacg ctattggcaa
      301 atcttacctt tgggaacaac cagttatgga gattctcctt accagtcatt ttcagctttt
      361 gcagggaata ctcattttat tgatttcgat atattgattg ataaaggctg gctagcagca
      421 gaggatttac acggtgttga ctttgggcag aatcctacag aggtagatta cgcagccatt
      481 ttttatgctc gccgtccatt actggagaag gtagttgtca acatgaaggc acaaggcttg
      541 ccagaagatt actggatgtt cttaggggag aatgactttt ggattcatac ctttgcggag
      601 tatatggcta taaaagagca ttttgacctc aaaccttgga cagagtggga agatcaaggg
      661 gcgcgcttac gttaccacga taccttggaa tattatcgtc acttgttggc agatcgaatt
      721 gattatcacc gcattacaca atatttattc ttttcacaat ggaaggcctt aaaggcttat
      781 gccaatgcca aaggtgttga gtttgttggg gatatgccga tttatatcgc ggctgattct
      841 gccgatatgt gggctaatcc acatttcttt aaaacagatg aagaaggcaa gccgagtgtg
      901 gttgcaggat gcccaccaga tgccttttcg gaaattggtc agctttgggg caatccgatt
      961 tatgattggg aagcgatgaa gcatgatgga tttacttggt gggttgctcg tttacgtgaa
     1021 tcctttaaga tatacgatat ggtacgtatc gaccacttta tcggctttgc ctccttctgg
     1081 gaaattccag cgggtgaaga aacagctgta aatggctatc gtgtcgaagc accaggtttt
     1141 gagttatttg atacaatcaa acgtgaactc ggagacttaa atatcatagc tgaggatttg
     1201 ggctctgtga cagacaaggt catccaatta cgtgatacga caggttttcc tggtatgaaa
     1261 gtcctgcagt ttgcttttga tccagaagga gacagcattg aaatgccaca taaccatcct
     1321 aataatgtgg tggcatatac gggaacccat gacaatgata cgattcttgg ctggtacgaa
     1381 aatgaaacaa cgaaagaatc gcgtgcattc ttagataagt acagcaatcg tcgtgagggc
     1441 gaaacggtca gtcatgctct tttccgcctg ctctttggtt cacctgcctt tatggcggtg
     1501 gctactatgc aggacctact tggtttggat ggttcagctc gcatgaactt gccaaatacg
     1561 cttggtggta actggacttg gagaatgacg gcggatcaat tgacaccaga gattgaagcg
     1621 gaattgctag accttacaga aacttatcgt cgggttaatg tgcatttggt tgatgaaaag
     1681 tcataataaa atgaactcct ctaagtataa cttagaggag tctttgt
//
LOCUS       DBNFEG010000227         1704 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.229, whole genome shotgun sequence.
ACCESSION   DBNFEG010000227 DBNFEG010000000
VERSION     DBNFEG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.229"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(124..1401)
                     /locus_tag="AC1PNG_002028"
     CDS             complement(124..1401)
                     /locus_tag="AC1PNG_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HIP6702486.1"
                     /translation="MSTKANLLFDVHEKPAPLQGILLSFQHVFAMFGATILVPLILGM
                     PVSVALFASGVGTLIYQVATQFKVPVYLGSSFAYISAMALAIKEMGGDVSAAQTGILF
                     VGLIYVLIAALVKVIGTKWIDSLLPPIVIGPMIIVIGLGLANSAVTSAGFVADGDWKN
                     VVVAIATFLIAAFVNTKGKGFAKIVPFLIAIIGGYVIAIFLGLVNFTPVLEAAWFELP
                     GFYLPFETGAFKSYNFYFGPEMIAILPIAVVTVAEHIGDHTVLSQICGRQFLKDPGLS
                     RTLIGDGVATAVSAFIGGPANTTYGENTGVIGMTRIASVSVIRNAALIAIAFSFLGKF
                     TALISTIPSAVLGGMSILLYGVIASNGLKVLIESRVDFGQVRNLIIASSMLVLGLGGA
                     VLNIGAITLSGTALSAIVGIILNLILPKAEKAE"
     gene            complement(1516..>1704)
                     /locus_tag="AC1PNG_002029"
     CDS             complement(1516..>1704)
                     /locus_tag="AC1PNG_002029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702487.1"
                     /translation="CTGRWLWHEKSSFKVSACGKRVGRRNGVIISSSASCSAIFLKNK
                     NLQNSLPKVRAYVTIPKA"
BASE COUNT          533 a          400 c          361 g          410 t
ORIGIN      
        1 aatatgacag gagaattaaa caactaaacc tttcacactc ctcgaaaact aattatttca
       61 ccccaatctc ctctacacca tcaaaaaaag ccagccgggg ctgacttgct gttattttca
      121 attttactca gccttttctg cttttggcaa gataagattg aggataatac ctacaatggc
      181 tgagagggct gtacctgaaa gggtgatggc accgatgttg agaactgctc caccaaggcc
      241 caataccagc attgagcttg caataatcag gttgcgaact tgaccaaagt caacacggct
      301 ttcaatcaaa actttcaaac cgtttgaggc gataacaccg taaagtaaga tagacatacc
      361 accaagaacg gcacttggaa tagttgagat aagggctgta aatttaccca agaatgagaa
      421 ggcaatggca atcaaggctg cattgcggat aacggatact gatgcgatgc gagtcatacc
      481 gataacccct gtattttcac cgtaggtcgt attggctggt ccaccgataa aggcagatac
      541 agcagtcgct acaccatctc caatcaaggt acggctgagg cctggatctt tcaagaactg
      601 gcggccgcag atttggctga gaacggtgtg gtcaccgata tgctcagcta ctgttacaac
      661 agcaattggt aagatagcaa tcatttctgg accgaagtag aaattgtacg acttgaaggc
      721 acctgtttca aatggtaggt agaaacctgg taattcaaac caagccgctt ccagaacggg
      781 tgtaaagtta accaggccaa ggaagatggc aatcacataa ccaccgataa tggcaatcaa
      841 gaaaggaaca atcttggcga agcctttccc tttggtattg acaaaagccg caatcaagaa
      901 ggtcgcaatc gccacgacga cgtttttcca gtcgccgtct gcgacgaagc ctgctgaagt
      961 cacggcagag ttggcaagac ccagaccgat gacgataatc attggaccga tgacaatcgg
     1021 tggcaagagg ctgtcaatcc acttagtacc gataactttg acaagtgcag cgatcaacac
     1081 gtaaatcaag ccgacgaaga gaataccagt ctgagctgcc gacacatcac cacccatttc
     1141 cttgatagca agcgccatag cagaaatgta ggcaaaggat gaacccaggt aaactggaac
     1201 tttaaactgg gttgccactt gataaatcaa ggtcccaaca cctgatgcga agagggcaac
     1261 cgatacaggc ataccgagaa tcagtggcac caaaatcgtc gcaccaaaca tagcgaacac
     1321 gtgctggaaa ctcaacaaaa tcccttgaag cggtgctggt ttctcatgga catcaaacaa
     1381 aagattggct tttgtactca taaatctccc attctgggta cacaaaaaga cccagacttt
     1441 ctaaatatac atagtcatag gcccttaatg aattatcctg ttgtctttct cacctcacgg
     1501 gatgagttta aaaacctacg cttttggtat tgtaacataa gcccttactt ttggcaagga
     1561 attttgtaaa tttttatttt ttaggaaaat ggcagagcag gaagctgaac tgcttattat
     1621 tactccattt cttcttccaa ctctttttcc acacgcgcta actttaaacg aagatttttc
     1681 atgccatagc catcttcctg tgca
//
LOCUS       DBNFEG010000228         1700 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.230, whole genome shotgun sequence.
ACCESSION   DBNFEG010000228 DBNFEG010000000
VERSION     DBNFEG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.230"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..684
                     /locus_tag="AC1PNG_002030"
     CDS             <1..684
                     /locus_tag="AC1PNG_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HIP6702488.1"
                     /translation="KSTADALTIGYNKPSYIFEDDALYYSEQSKYRKENPKRLDIVLK
                     TKYHLSRLILEKQDDATIKNQINQYGVVLPWTVFRLMTFGNIASFLVALQPEYRNKVA
                     AYISLPLDKDDNIPAKILLSWCNALRYLRNICSHNGRLYGRLHHTLPAFHHSDRELLV
                     TDSENDSKTLFIYFIAMRHLILSMSIETKNFWNKKLQKLLEESNQEQVDLRHYGFPKN
                     WMDLLAIKI"
     gene            738..980
                     /locus_tag="AC1PNG_002031"
     CDS             738..980
                     /locus_tag="AC1PNG_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702489.1"
                     /translation="MNLISIKEFVELTINNNPDINPKELEETLRAVLEEKEGGARCMN
                     CGSPIWVAGSALVGSYMCFTCLTGEADGSDDFEVLG"
     gene            1321..1680
                     /locus_tag="AC1PNG_002032"
     CDS             1321..1680
                     /locus_tag="AC1PNG_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1252 family conjugative relaxosome accessory
                     protein"
                     /protein_id="HIP6702490.1"
                     /translation="MGQEIKLIRKQFRITRQEEKQIKEMMREQNLDSFSEFLRQNLLK
                     KNYQDRIFESWFSLWQSQKFEQISRDVHEVLVVARENHQVTQEHVSILLTCVQELIAE
                     VNQVQPLSRGFREKYMR"
BASE COUNT          561 a          272 c          360 g          507 t
ORIGIN      
        1 aagagtacag ctgatgctct gacgattgga tacaataaac cttcttatat ttttgaagat
       61 gatgctctct attattcaga acaatctaaa taccgaaaag aaaatcctaa gaggctggat
      121 attgttttga aaacaaaata tcatttgtca cgattgattt tggaaaaaca agatgatgcc
      181 acaatcaaga atcagattaa ccaatatggg gtcgtgttac cttggaccgt ttttcgttta
      241 atgacatttg gaaatatagc gtccttcctc gttgccctac aaccagaata tcgaaataag
      301 gttgctgcct atattagtct tcccttggac aaggatgata acattccagc taaaatctta
      361 ttgtcttggt gtaacgctct tcgttatctt aggaatattt gctcacacaa tggcagatta
      421 tatggtcgct tacaccatac actgccagct tttcatcatt ctgacagaga gttgctagtg
      481 acagactcag agaatgatag caagacacta tttatctatt ttatagcaat gagacatctt
      541 atcttgtcaa tgtccataga aactaagaat ttttggaata aaaaattaca aaagcttctg
      601 gaagaaagta atcaagaaca agtggatttg agacactatg gttttccaaa aaattggatg
      661 gatttattgg caataaagat atgataagtg cttagatgga cacggactaa aaaatagatt
      721 agaacggagg taaagtattg aatcttattt caatcaaaga atttgtcgaa ttgactatca
      781 ataataatcc agacatcaat cccaaagagc ttgaagaaac cttgcgtgca gtacttgaag
      841 aaaaagaagg aggggcaagg tgtatgaact gtggatctcc tatttgggta gcagggagtg
      901 cactggtagg gagctacatg tgcttcacgt gtctaacagg tgaagccgat ggtagtgatg
      961 attttgaagt tttaggttaa gctatttcat ttcagaaatt tttcgagagc caaatggctc
     1021 tttttctttt cctgtgataa ttttttcagc tctctcttgt tacactctat ctagttttag
     1081 aaaatcaacc cttgactttt tcttgggggt ttgggggcga agccaccaag ttatcttatc
     1141 gtcagctatc aaaactcaaa ggttttggtc agctggcgat atgattttgg ggatattgtg
     1201 gacacaatat ctgagctcgc aaagaccgaa caagaagaga gtgaggtctt taggaagcgt
     1261 actgacaatg tgaggcagcc ttacattgta agacaagtat catgttgaaa ggatagtagg
     1321 atgggacaag aaattaagtt aatccgtaag caatttagga tcacgagaca agaagaaaaa
     1381 cagataaaag aaatgatgag ggaacaaaat ctggatagtt tctcagaatt tcttcgtcaa
     1441 aatttgttga aaaagaatta tcaggataga atttttgaaa gttggttttc cctttggcaa
     1501 tctcaaaagt ttgaacaaat tagtcgagat gtgcatgaag ttctagttgt cgcaagagaa
     1561 aatcaccaag tgactcaaga acacgtgtca atcttattga cctgcgttca agaattgatt
     1621 gcggaagtca atcaagtaca gccactcagt cgtgggtttc gtgaaaaata catgagatag
     1681 gaggctacta tggtttatcg
//
LOCUS       DBNFEG010000229         1695 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.231, whole genome shotgun sequence.
ACCESSION   DBNFEG010000229 DBNFEG010000000
VERSION     DBNFEG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.231"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>1695
                     /locus_tag="AC1PNG_002033"
     CDS             <1..>1695
                     /locus_tag="AC1PNG_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HIP6702491.1"
                     /translation="KVNEQYFYYPKDKDANVISISEAKKLTKNQKKPTSQAKKGVAGV
                     DYPTDDGFLFENEGQILSKTDEGLVLEHNGHSHFIFYKDLKESKWSYLVPKEYIENSQ
                     SSSHSNAKQAKLSQTFDDGYVFDPKDIVAEDANGYTVRHGDHYHYIWKSSLQYGQGNT
                     GETIGKKGQITVLDNHSLHTPTTRNNFVPLKSTTEKGHTSTQSNSKKAFPGIDYPTSD
                     GFIFDGSGVQGQTALGLLIGHGTHTHLLPYSHLIGSPWESYIPTQYLETARAEYHSST
                     STQVDVEIPLSPQEGVKPNSSVEEEREAKKSYLAENMHVSKEKIKVIETDAGPAFVYP
                     HGDHSHTILIEKVEVGKPIEDPHGDPHAHDKIGMATLKQLGFDDEIIEDILHATADTP
                     FPSNETDSEKMREWLKTVKYLNIGQRKDPLKRAGLDLMPNVEVLGIGFTPIDDVKPIL
                     QFKKLKQLWMTSTGVKDYQFLKEIPTLEGIDLSQNGVSDLGFLEEFPNLKVVSAAGND
                     IEDITILAKLKALESLNLDHNKVTDLSPLADLSQLTAVSLDNNRITDLSALQNKKK"
BASE COUNT          583 a          291 c          339 g          482 t
ORIGIN      
        1 taaaagtaaa tgagcagtac ttctactatc ccaaggataa agatgctaat gtaatttcta
       61 tttctgaagc taagaaactc accaaaaatc aaaagaaacc tacttctcaa gctaaaaaag
      121 gggtagctgg tgttgattat cctacggatg atggatttct ttttgaaaat gaaggtcaaa
      181 ttctttcaaa aacggatgag ggactggtct tagaacataa tggtcattca cattttattt
      241 tctataagga tttaaaagaa agcaaatggt cctatctggt acccaaagag tacattgaga
      301 atagccaatc cagctcgcat tccaacgcaa agcaagcgaa gctatcacaa acttttgatg
      361 atggttatgt atttgatccg aaggatatag tggctgaaga tgcgaatggc tatacggtac
      421 gtcatggaga tcattatcat tatatttgga aatcaagttt gcaatacgga caaggaaata
      481 ctggggagac cattggaaaa aagggacaga ttactgttct tgataatcat tcgttgcata
      541 ctccgaccac aagaaataat ttcgtcccac tcaagtctac cacagaaaaa gggcatactt
      601 ccactcagtc taattcaaaa aaagctttcc caggtattga ttatccaaca agcgacggtt
      661 tcatttttga tggtagtgga gttcaaggtc aaacagcgtt agggttactc attggccatg
      721 gtacgcatac tcatttactt ccgtacagtc atttgatagg aagtccgtgg gagtcttata
      781 ttcctactca gtatttagag actgctagag cagaatatca ttcgtcaact tccactcaag
      841 ttgatgtaga aattccgttg tcaccacaag aaggtgtgaa acctaactca agtgtagaag
      901 aagaacgaga agctaaaaag agctacctag ccgaaaacat gcatgtttca aaagaaaaaa
      961 tcaaggtgat agaaacagat gcaggacctg ctttcgttta ccctcatggt gaccattctc
     1021 atacaatctt gattgagaaa gttgaagttg gaaaaccaat cgaagaccct cacggtgacc
     1081 cacatgctca tgataaaatt ggaatggcta ctttaaaaca attaggtttt gatgatgaga
     1141 ttattgaaga tattttgcat gctacggccg atactccttt cccatctaat gaaacagatt
     1201 ccgagaagat gagagagtgg ttaaaaacgg taaaatatct aaatattggt cagcgaaaag
     1261 atccgctaaa gagagccggt ctggacttga tgccaaatgt tgaagtgtta gggattggtt
     1321 ttacgccaat tgatgatgta aaacctatct tgcaatttaa aaaattgaaa caattatgga
     1381 tgactagcac aggcgttaag gattatcagt ttttgaaaga aattccaaca ttagagggaa
     1441 ttgacttatc acaaaatggt gtttctgatt tgggcttttt ggaagaattt ccaaacttaa
     1501 aagtagtttc tgctgcagga aatgacattg aagatatcac tattctagct aaattaaaag
     1561 ctttggaatc cttaaattta gatcataata aagtgacgga tttatcccca ttagcagatt
     1621 tgtcacaatt gacagctgtt agtttagata ataatcgtat cacagattta tctgcgcttc
     1681 aaaacaagaa gaagt
//
LOCUS       DBNFEG010000230         1675 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.232, whole genome shotgun sequence.
ACCESSION   DBNFEG010000230 DBNFEG010000000
VERSION     DBNFEG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.232"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..928
                     /locus_tag="AC1PNG_002034"
     CDS             <1..928
                     /locus_tag="AC1PNG_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HIP6702492.1"
                     /translation="SALSLELGEMTETIKQVTYIFPKFAEKRLTWEDTLGTANLTISE
                     KVGWQFGSPPHVLLAGSTKSGKTVLLENLVAQYLNIGAEIKLLDPKNGELSWLVGKKL
                     EDRLGYKVVYNSPFQIAGALREAVREMNIRFQVMADNPDTYISKGKVLSWADVEGNYP
                     LVIVLDEGIAFKTEAETTKEGKQAYQEAMSNLGSLLVKSRQASIEVIVGLQRASSDFI
                     PTYMRQNFGVALLLGSTTSDLDSCRMMFSSQDIDYKTCDIGTGYIQIDGVIPQPRYIE
                     TPFKSDELDFEEYFDKACDCYWSIRNNDGLSD"
     gene            1205..1624
                     /locus_tag="AC1PNG_002035"
     CDS             1205..1624
                     /locus_tag="AC1PNG_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702493.1"
                     /translation="MTYNMTDTTLKIGQLDLDMTQFEVPKKIVILSAKVNNRYNEVNG
                     EKIKTEEVTKITCTALDADKVKVLTEMGISTDDLKAINLEIVGNVDKVATLAQNESLL
                     NVPIELVKPKVRLAWNMARSNWAGVKLVCEDIKILGA"
BASE COUNT          520 a          283 c          401 g          471 t
ORIGIN      
        1 tagtgcactc tctctagagt tgggagagat gacagagact atcaagcaag tgacctatat
       61 ttttccgaag tttgctgaga agagattgac ttgggaggat actctaggaa ctgcaaattt
      121 gacaatctct gagaaagtgg gttggcagtt tggctcaccg cctcacgtcc ttttggctgg
      181 ttcgaccaag tcggggaaaa cggtgctcct agaaaacctt gtagcccaat acttgaatat
      241 cggagctgaa ataaagttgc tcgacccaaa gaacggggaa ctttcttggc ttgtcggtaa
      301 aaagttagag gataggttag gctataaagt tgtttataat agcccttttc aaattgcagg
      361 agctttgaga gaagctgtca gggaaatgaa tattcgcttt caggttatgg cggataaccc
      421 agatacctat atttctaaag gaaaagtttt atcatgggcg gatgtcgagg gcaattatcc
      481 tcttgttatc gtgcttgatg aggggattgc tttcaaaaca gaggcagaaa cgactaaaga
      541 agggaaacaa gcctatcaag aggcaatgtc gaaccttgga agtctattag taaaatctcg
      601 ccaagcctct atcgaagtta tagtaggtct gcagagagcc tcaagcgact tcatacctac
      661 atatatgagg cagaattttg gggttgctct gttattgggt tcaacgacct cagacttgga
      721 ttcttgtcgc atgatgttct caagtcaaga tattgattac aagacttgtg atattgggac
      781 aggctacatt caaatagacg gagtgattcc acaacctaga tatattgaaa caccttttaa
      841 gtctgatgaa ctagactttg aggaatattt tgataaggct tgcgactgct actggagtat
      901 tagaaataat gacggtcttt cggactagcg aatagagacg attgcccagg gggcagaatc
      961 gagcggagcg ttcctctttt cgctaggaga aagagccaac cgactgcttt tgcaggaggt
     1021 cagaaaaata cccccattac taataggggg tgcacacata catgaaatgt actctaaacc
     1081 cttgatacgt aaggggttac ggcttgttgg ttataccgaa gtcattgaaa cccttgtggg
     1141 agaagggatt gagtcgcttt tcgtgtagga tacgactttt tgaatagaaa atggagtaat
     1201 tattatgact tacaatatga cagatactac ccttaagatt gggcaacttg atttggatat
     1261 gacacagttt gaagtgccga aaaaaattgt tattctatct gctaaagtaa ataataggta
     1321 taacgaggtt aatggcgaga aaatcaaaac tgaagaagtt acgaaaatca cttgtactgc
     1381 ccttgatgcc gataaagtga aagttctaac tgaaatggga atttctactg atgacttgaa
     1441 agctatcaac ctagaaattg taggtaatgt agataaggtt gctacgctcg ctcaaaatga
     1501 atcacttttg aatgttccga ttgagttggt taagccgaaa gttcgtttag cttggaacat
     1561 ggcacgaagt aactgggcag gtgttaaatt agtatgtgag gacattaaaa ttctaggagc
     1621 ataaccctat gaatacagtc agaattgatt attttgcagt aaccgttaaa aatat
//
LOCUS       DBNFEG010000231         1650 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.233, whole genome shotgun sequence.
ACCESSION   DBNFEG010000231 DBNFEG010000000
VERSION     DBNFEG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.233"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1083
                     /locus_tag="AC1PNG_002036"
     CDS             <1..1083
                     /locus_tag="AC1PNG_002036"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIP6702494.1"
                     /translation="EVVSSILEDFKTRENQSYILIIDGVENIIPQCTRYRVLNKAEQL
                     RELGYTVRVINNSLVQLQDAQFASHIIIYRAPFNDMLKEICRAAHIKNRPVYFDIDDL
                     VFDTKFTDELEFTQGLSKREKKGYDTSVLAYKKMLSLCDYAITSTSKLKDELEQYKNK
                     VILNRNVMSKELVERSLQVKKHSNDNKVKIGYFSGSITHNENFDLISQALLHLLQKYP
                     QVELHIVGYLDIPKPFQKFKKQIVSHEYVDWRKLPILISQVDINLAPLVTTTFNEAKS
                     EIKWIEAAAVKVVTVASNLGAFEEMIQDGVTGVLADDNEWESKLERLILEQDLREQIA
                     ENAFEFVMNHCTTANRINDFLKEELV"
     gene            1083..>1650
                     /locus_tag="AC1PNG_002037"
     CDS             1083..>1650
                     /locus_tag="AC1PNG_002037"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIP6702495.1"
                     /translation="MKRILFISPTTSMDNGAEKSIYYLMKHLIQLGHTVINVTHAIGG
                     EPQDKHEQLYKMIGVKNYYLPTVKWWWPDAPGGEILNREEATSYYRQNVQDIAEIIDQ
                     NEIDLVISNTVNVFQGAIAASCQKVPHFWLIHEFPKNEFAYYADKIDFIEEYSSELFS
                     VTGELNNFLTPLFNKKVHSFISYTEQETK"
BASE COUNT          587 a          235 c          321 g          507 t
ORIGIN      
        1 gaagtggtgt cttctatatt agaagatttt aaaactagag aaaatcaatc ttatattttg
       61 attattgatg gtgtcgaaaa tattattcca caatgtacac gttatcgtgt gttaaataag
      121 gctgagcaat tgcgagagct tggttataca gttcgagtga taaataattc tcttgtacaa
      181 cttcaggatg cacagtttgc tagtcatatt attatttatc gtgctccatt caatgatatg
      241 ctgaaagaaa tatgtagggc agcacacata aagaacagac ctgtttattt tgatattgat
      301 gatttagttt ttgatacaaa atttacagat gaacttgaat ttacccaagg tttgagcaag
      361 agagagaaaa aaggctatga tacaagtgtt ttagcttaca aaaaaatgct ttcactctgc
      421 gactatgcta tcacatcgac ttcgaaattg aaagacgagc ttgaacaata taaaaacaag
      481 gttatcttga atcgcaacgt catgtctaaa gaattagttg agcgtagttt acaagttaag
      541 aaacattcga acgacaataa agttaaaata ggatatttct cagggtcaat tacacacaac
      601 gaaaattttg atttaattag tcaagcattg ctacatctac ttcaaaagta cccgcaagtg
      661 gaattacata tagtaggata tttagacatt ccgaaaccat tccaaaaatt caaaaaacag
      721 attgtgagtc atgagtatgt tgattggaga aagcttccaa ttttaatttc acaagttgat
      781 attaacttgg cgccgcttgt aaccacaacc tttaatgaag caaagtcgga aattaaatgg
      841 atagaagcgg cagcagttaa agttgtgaca gttgcaagta atcttggagc atttgaagag
      901 atgattcaag atggagtgac tggagttcta gcagatgata acgaatggga aagtaaattg
      961 gagagattga ttcttgaaca agacttgaga gaacaaatag cagaaaatgc ttttgagttt
     1021 gtaatgaatc attgcactac agcaaataga attaatgatt ttttgaaaga agagttagtc
     1081 taatgaaacg tattttgttt atttctccta cgactagtat ggataatggt gcggagaagt
     1141 ctatttatta tctaatgaag caccttattc aactgggaca cacagttata aatgttactc
     1201 atgcaatagg aggggaacca caagataagc atgagcaact ttataaaatg ataggggtta
     1261 agaattacta tttgccaaca gtcaagtggt ggtggccgga tgcaccaggt ggagaaatcc
     1321 ttaatagaga agaggctaca agctactatc gccagaatgt tcaagacata gctgaaatta
     1381 ttgatcaaaa tgagatagac ttagttattt caaatactgt taatgttttt caaggagcaa
     1441 ttgctgcctc atgtcaaaag gttccccatt tctggctaat tcatgaattt cctaaaaatg
     1501 agtttgctta ttatgctgat aaaattgact tcatagaaga gtattcttca gaattattta
     1561 gtgtgactgg agagttaaat aattttttaa caccactatt taataagaaa gtccattcgt
     1621 ttatttctta tactgagcaa gaaacaaaag
//
LOCUS       DBNFEG010000232         1631 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.234, whole genome shotgun sequence.
ACCESSION   DBNFEG010000232 DBNFEG010000000
VERSION     DBNFEG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.234"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            180..1568
                     /locus_tag="AC1PNG_002038"
     CDS             180..1568
                     /locus_tag="AC1PNG_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIP6702496.1"
                     /translation="MSLFRKKQAVGRHSQMRRHLGLVDLIFLGIGSMVGTGIFTVTGL
                     AAAQYAGPALIISIVIAAISVGLTALFYAEFASRIPTNGGAYGYLYSVFGEFPAWIAG
                     WLTIMEFLTAVSSVASGWGAYLKGLLANFGIAMPTALNGTFNPAAGTYVDLLPVLVLI
                     FVVGVVLLNSKAALRFNSALVVLKFSALALFILVGLFFIKPENWSNFSPFGFGAIYGG
                     QAGIMAGASLMFFAFLGFESISLAIDEVKKPEKNVPKGIVLSLSIVTILYIVVTLVLT
                     GMVHYTKLNVADAVAFALREVGLDWAASYISIVAILTLITVCISMTYALSRMVYSISR
                     DGLLPKSLSRLTQTSKVPKNATILVGIFAAICAGIFPLASIASFLNICTLAYLIMLAL
                     GIIRLRQVEGLPKDGQFKTPLVPLLPILSIIICLSFMFQYSLDTWLAFGVSVVIGILI
                     YFCYGYRHSEAK"
BASE COUNT          390 a          289 c          365 g          587 t
ORIGIN      
        1 ttaaggttaa tccagaataa tcataagaaa gagtgaggta gaaaagcctt gctcttttgc
       61 ttgtcgtcac ccatgcgaaa tatttcaact tatggtataa tggggcgttg tggttctttg
      121 acaggaatcc gtagattatt ctgtcgagta tccatattct tattctgaag gagtattttg
      181 tgagtttatt tagaaaaaaa caagcagtcg gtcgccatag tcaaatgcgt cggcatttag
      241 gcttagttga tttgatattt ttaggtatcg gttcgatggt tggaacaggt atctttacgg
      301 taactggttt ggcagcagcc cagtatgcag ggccagctct gattatctca attgttattg
      361 cagcaatttc agtcggacta acagccttgt tttacgcaga atttgcctcg cgtataccga
      421 ctaacggtgg tgcctatggt tatctctact cggtttttgg tgaatttcct gcctggattg
      481 ctggctggct gacgatcatg gaattcttga ctgctgtatc gagtgttgca tctggttggg
      541 gagcttattt gaaaggactc ctggctaatt ttggtatcgc tatgccgaca gctttgaatg
      601 gaacttttaa ccccgctgca ggtacctatg tagacctctt accagttctc gtcttgattt
      661 ttgtggtagg agtggtcttg cttaattcta aggcagcact tcgtttcaat tcagccttgg
      721 tagttttgaa attttcagcg ttggctttat ttattttagt agggttattc tttatcaaac
      781 cagaaaactg gtcgaacttc tctccttttg gttttggtgc catttatggt ggacaggcag
      841 gaattatggc aggagcgtct ctcatgttct ttgccttcct gggatttgag tctatttcat
      901 tagcaattga tgaggtgaaa aaacctgaga agaatgtacc aaagggaatc gtgttgtctc
      961 tatccattgt aacaattctt tatattgtag tgacattagt cttgactggt atggttcact
     1021 atactaagtt gaatgtggca gatgcggtag cctttgcact tcgagaagtt ggattggatt
     1081 gggcagcgag ttatatttct attgttgcaa tcctaacatt gattacagta tgtatttcga
     1141 tgacctacgc cttgtcgaga atggtctatt ccatcagtcg tgatggtttg ctaccgaaat
     1201 cattgagccg attgacacaa acaagcaagg ttcctaaaaa tgcgaccatt cttgtgggga
     1261 tttttgcagc catttgtgca ggtattttcc cactagcaag tattgcttcc ttcctcaata
     1321 tctgtacctt agcttatttg attatgctgg cgttgggtat tattcgtctg cgtcaagtcg
     1381 aaggtttgcc aaaagatgga caatttaaaa cacctcttgt tcccttgtta cctatcctct
     1441 cgattatcat ttgtctttca tttatgttcc agtatagttt agatacttgg ttagcattcg
     1501 gagtatccgt ggttattgga attttgattt acttctgtta tggctatcgt cattcggaag
     1561 caaaataata gatttccctt ctttatttga agggattttt tgtacaattt ttttatagaa
     1621 ataatggaaa a
//
LOCUS       DBNFEG010000233         1614 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.235, whole genome shotgun sequence.
ACCESSION   DBNFEG010000233 DBNFEG010000000
VERSION     DBNFEG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.235"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            20..1048
                     /gene="queA"
                     /locus_tag="AC1PNG_002039"
     CDS             20..1048
                     /gene="queA"
                     /locus_tag="AC1PNG_002039"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIP6702497.1"
                     /translation="MNTADFDFDLPEELIAQVPLEKRDSSKLLILDREKQSMVDSHFD
                     HIIDQLNPGDALVMNNTRVLPARLYGYKPETMGHVELLLLKNTQGNQWEVLAKPAKRL
                     KVGTTVAFGDGRLTATIVEELEHGGRIVEFSYEGIFLEVLESLGEMPLPPYIHEKLED
                     RERYQTVYAKENGSAAAPTAGLHFTQELLEKIEAKGVKLVYLTLHVGLGTFRPVSVDN
                     VDEHEMHSEFYNLSAEAAQTLNQVKDSSGRIVAVGTTSIRTLETIGNKFDGRLEADSG
                     WTNIFIKPGYTFRIVDAFSTNFHLPKSTLVMLVSAFAGREFTLDAYKHAVEERYRFFS
                     FGDAMFIQ"
     gene            1097..1570
                     /locus_tag="AC1PNG_002040"
     CDS             1097..1570
                     /locus_tag="AC1PNG_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIP6702498.1"
                     /translation="MNKIESRHQLILSLIMEKKIHTQQELQELLEINGVSVTQSTLSR
                     DIKMLNLVKVNEDDSSHYVINPIAPTRWEKRLRLYMEDALVMLKPIQHQVVLKTLPGL
                     ANSFGSILDAMEIPQIVATVCGDDVCLIICEDVEGAQACFEHLKQFTPPFFFSKL"
BASE COUNT          463 a          372 c          323 g          456 t
ORIGIN      
        1 tttagtctaa ggagtgaata tgaatactgc agattttgat tttgatttac ccgaagagct
       61 tatcgctcaa gttccccttg aaaagcgcga cagctctaaa ctattgatcc tcgaccgcga
      121 aaaacagagc atggttgaca gtcactttga ccatatcatc gaccagttga atcccggtga
      181 tgccctggtc atgaacaata cacgtgttct gcctgcccgt ctctatggct acaagcctga
      241 aactatggga catgttgagc ttttgctttt gaaaaatacc caaggtaatc agtgggaagt
      301 tcttgccaaa ccagcaaaac gtctgaaggt cggtaccaca gtcgcttttg gtgacggacg
      361 attaactgct actatcgtag aagaattaga acacggtggt agaattgttg aattctccta
      421 tgaaggtatc ttccttgaag tcttggaaag tctaggcgaa atgccacttc caccttacat
      481 tcatgagaaa ttagaagacc gcgaacgcta tcaaacagtt tatgccaagg aaaacggttc
      541 tgccgcagct ccaactgctg gactacattt cacccaagag ttactcgaaa aaatcgaagc
      601 taaaggagtg aaactagtct atctgactct tcacgttggg cttgggacct tccgaccagt
      661 ctctgttgat aacgttgatg agcatgaaat gcactcggaa ttctataacc tatcagcaga
      721 agctgctcaa accctcaatc aggtcaaaga tagcagtggt cgtatcgtcg cagtcggcac
      781 gacatccatc cgcaccctcg aaaccatcgg taataaattt gatggtcgtt tggaagctga
      841 ttctggttgg acaaatatct tcatcaaacc tggctacacc ttccgaatcg ttgatgcctt
      901 ctcaaccaat ttccacctgc caaaatctac tttggttatg ctagtttctg cctttgcagg
      961 tagagaattt accttagatg cctataaaca tgctgttgaa gaacgctacc gcttctttag
     1021 ttttggggat gcaatgttca ttcaataggc tgttatcacc aaattattct gaattctgat
     1081 tttattggag ggccctatga acaagattga aagccgccat cagctgatcc tgtctctaat
     1141 catggagaag aaaatacata cccaacagga attgcaagaa ttactagaga tcaacggagt
     1201 ctccgtcacc caatctaccc tctcccgtga catcaagatg ctcaatcttg tcaaagtcaa
     1261 cgaagatgat tcttctcact atgtcatcaa ccccattgct cctactcgct gggaaaaacg
     1321 actgcgactc tatatggaag atgctttggt catgctaaaa cctatccaac atcaagtggt
     1381 gctcaagacc ctacctggat tggccaactc attcggttct attttggatg ccatggaaat
     1441 tcctcaaatt gtagcgaccg tctgcggtga cgatgtctgt ctcatcattt gtgaagatgt
     1501 cgagggggca caagcttgct tcgagcactt gaaacagttt actccaccat ttttctttag
     1561 taaattataa aaatttatgt tcacaaacaa atacttaatt ttaaagataa tttt
//
LOCUS       DBNFEG010000234         1608 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.236, whole genome shotgun sequence.
ACCESSION   DBNFEG010000234 DBNFEG010000000
VERSION     DBNFEG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.236"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(135..>1608)
                     /locus_tag="AC1PNG_002041"
     CDS             complement(135..>1608)
                     /locus_tag="AC1PNG_002041"
                     /inference="COORDINATES: protein motif:HMM:NF039499.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HIP6702499.1"
                     /translation="YLMFSKLSDHDFKKGVFRTKFSQIVTPLDKESDWIHSKGPEYLW
                     IGLALQYGSRTEQMERMMLALTNLSKELDLEKILPLPAMSLILSLDIEEKKIFVESLN
                     SAFDLSIFSPLSIVLPDGEEVLSKNFYNRSHSFDQRLDVLVKVLDEISDQYSQLSTDV
                     RYFLLYYKMLQGKIKFVESQSHMVDGLTKYPYLDISDPEMKIIRSQIRSMEVALSMSE
                     GMNYLYSKRFWNNISQLTDCEVYSIVMDRSNAIDLNEIKGTVYSALKYYRDMLQNIEP
                     FNEKLYVLTSILTYSYKRLLELMNHDLQYTISGRSIVRSCIENYVMTKYLIAEEANHK
                     NIWEEYQYYGIGGYKLVSERFIENQPELTNPHINFEYLNLLVSEFQNKEFIDMDTRYF
                     GNGNIRQKFKQVGEDDLYKFYYDYDSQFEHGLWGAIRESSVLKCTSAGHQFHGIPDID
                     DVQELPDISHDMVMILTKHLSVINEVFPIPSERRDGDRLC"
BASE COUNT          530 a          301 c          197 g          580 t
ORIGIN      
        1 ttctagtaat ttttgttttc taaaccaaaa aattctaatt ttatctaaat gataagctac
       61 atctcctaag tctaagtcgc ctctaactct cctcagcaac tctaaatact cattttgtga
      121 tttcctaagt ttatctagca taatctatca ccatctcttc gctcacttgg aataggaaat
      181 acttcgttaa tgacagataa atgctttgtt aatatcatta ccatgtcatg actaatgtca
      241 ggtaattcct gtacatcatc tatatcagga attccatgaa attgatgccc agctgatgta
      301 catttcaaaa cagaactctc tctaatggct ccccaaagtc catgttcaaa ttgagaatca
      361 taatcatagt aaaatttata taaatcgtct tcccctactt gcttaaattt ttgtcttata
      421 ttgccatttc caaaatatct agtgtccata tctataaatt ctttattttg aaattccgac
      481 actagcaaat ttaaatattc aaaattaata tgaggatttg ttaactctgg ttgattctct
      541 ataaagcgtt cggaaactaa tttataacca ccaataccat aatattggta ttcctcccaa
      601 atgtttttat ggtttgcttc ctcagcaatt aaatattttg tcataacata gttctcaatg
      661 catgacctta ctatagaacg ccctgaaatc gtatattgca aatcatgatt catcaactct
      721 agtaaccgct tataagaata ggttaggata ctagttaaaa cataaagctt ctcgttaaaa
      781 ggctctatat tttgaagcat gtctctgtaa tattttaatg ctgaatatac tgtacctttt
      841 atctcattca aatctatagc attacttcta tccataacaa ttgaatatac ctcacaatca
      901 gttagttgac taatattatt ccaaaatctt ttactataaa gataattcat accttcagac
      961 attgataaag caacctccat tgagcgaatc tgggaacgaa taattttcat ttctggatct
     1021 gatatatcta agtaagggta tttagttaat ccgtctacca tgtgtgactg cgattctaca
     1081 aatttaattt ttccttgtag catcttgtaa tacagtaaaa aatatcttac atccgttgac
     1141 aactgactat attggtcaga tatttcatct aatactttaa caagtacatc tagtctttga
     1201 tcaaaagaat gactcctgtt gtaaaaattt ttactcagaa cttcttcgcc atccggcaag
     1261 actattgaaa gtggagaaaa tattgataag tcaaaagctg aatttaatga ttcaacaaaa
     1321 attttcttct cttcaatatc taaactaagt attaaactca ttgcaggtaa aggcaagatt
     1381 ttttctaaat ctaattcttt agataaatta gtcagagcta acatcattct ttccatttgc
     1441 tctgttcggc ttccgtattg cagagctaat ccaatccaaa gatattctgg accttttgaa
     1501 tgtatccaat cagattcttt atctaatgga gttactattt gactgaattt agttctaaag
     1561 accccttttt taaagtcatg atcacttaac ttactaaaca taagatac
//
LOCUS       DBNFEG010000235         1521 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.237, whole genome shotgun sequence.
ACCESSION   DBNFEG010000235 DBNFEG010000000
VERSION     DBNFEG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.237"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            37..1518
                     /gene="guaB"
                     /locus_tag="AC1PNG_002042"
     CDS             37..1518
                     /gene="guaB"
                     /locus_tag="AC1PNG_002042"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIP6702500.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPHDIDLKTQLAPNLTL
                     NLPIISAAMDTVTDSKMAIAMARAGGLGVIHKNMSIAEQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEAEKLMATYRISGVPIVETLENRKLVGIITNRDMRFISDYSQPISTNMT
                     SDALVTAPVGTDLATAEAILHKHRIEKLPLVDENGRLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIKEIREHFPTR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAGVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGHAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKQGSKDRYFQASVNEANKLVPEGIEGRVAYKGSVADMIFQMVGGLRSGMGY
                     VGAGNLTELHENAQFIEMSGAGLKESHPHDVQITNEAPNYSVQ"
BASE COUNT          431 a          294 c          367 g          429 t
ORIGIN      
        1 atcatgctac atattaaata agaaaagagg aagaaaatgt caaactggga cactaaattt
       61 ttgaaaaaag gctttacttt cgacgatgta ttacttattc cggctgaaag tcatgtatta
      121 ccacatgata ttgatttaaa aacacaatta gcaccaaatt tgacccttaa tcttccgatt
      181 atttctgctg ctatggatac agtaacagat agcaaaatgg ctatcgctat ggcgcgtgcg
      241 ggtggtttgg gcgtcatcca taagaacatg tctatcgcgg agcaggctga tgaggtgcgc
      301 aaggtaaaac gttctgaaaa tggagtcatt atcgatccat tcttcttaac accagaacac
      361 accattgctg aagctgagaa attgatggct acttatcgta tttccggtgt gccaatcgtt
      421 gaaacattag aaaatcgtaa gttggtcggt attatcacca atcgcgatat gcgtttcatt
      481 tcagattact cacagccaat ttcgaccaac atgaccagcg atgctttggt aacagcgcca
      541 gttggaacag atcttgcgac tgcggaggct attcttcaca agcatcgtat tgaaaaattg
      601 ccattggttg atgaaaatgg tcgtttgtca ggcttgatta ctatcaaaga tattgagaaa
      661 gtgattgagt tcccaaatgc tgcgaaggat gaatttggtc gtctcttggt tgcgggtgct
      721 gttggtgtta catctgatac ttttgaacgt gcagaagctc tctttgaagc aggtgcagat
      781 gcgattgtca tcgatacagc tcatggtcat tcagctggtg ttttgcgtaa gatcaaagaa
      841 atccgcgaac acttcccaac tcgtaccttg attgccggca acattgcgac tgcagagggt
      901 gcacgtgccc tttatgaagc aggggttgat gtcgttaaag ttggtattgg tccaggttcc
      961 atctgtacga ctcgcgtaat cgcaggtgta ggagttcctc aggtgacggc tatttacgat
     1021 gcggcaggtg ttgcgcgtga atatggtaaa acgattatcg ctgatggtgg tatcaaatat
     1081 tctggagata ttgtcaaggc acttgcagcg ggtggtcatg ccgttatgct tggttctatg
     1141 tttgcaggaa cggatgaagc accaggtgag acagaaatct tccaaggtcg taagttcaag
     1201 acctatcgcg gtatgggctc tatcgctgcc atgaaacaag gttcaaaaga ccgttacttc
     1261 caagcatctg tcaatgaagc caacaaactt gttccagaag gaattgaagg acgtgtagcc
     1321 tataaaggtt cagttgcaga tatgattttc caaatggttg gtggacttcg ctcaggtatg
     1381 ggctatgtag gtgctggtaa cttgactgaa ttgcatgaaa atgcacaatt catcgaaatg
     1441 tcaggtgctg gtttgaaaga aagccatcca cacgatgttc aaatcacaaa cgaagctcca
     1501 aactattcag ttcagtaaaa c
//
LOCUS       DBNFEG010000236         1513 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.238, whole genome shotgun sequence.
ACCESSION   DBNFEG010000236 DBNFEG010000000
VERSION     DBNFEG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.238"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..241)
                     /locus_tag="AC1PNG_002043"
     CDS             complement(<1..241)
                     /locus_tag="AC1PNG_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702501.1"
                     /translation="MRFCRILFFHKKSTIFRLFFIILLGSLIFSNTTYQYYLGHYQLV
                     PNVFTWLLILCQDITTGVYLIPIIYTAILFFCIEVF"
     gene            complement(238..1002)
                     /locus_tag="AC1PNG_002044"
     CDS             complement(238..1002)
                     /locus_tag="AC1PNG_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702502.1"
                     /translation="MKFLLMNVRRTVFSKKMLLSFLLAFSCILIGNMVAFNGIYKLEG
                     LSLFFAGSTIRGFSPISLVAAVISAIPIADRVIEDSKNHFLRLQLQRTSRIKYIWTLL
                     VTAGISGFLSLFLPYFLLLVANLCLTPYKEIYIGDYQGVFKSIFDSNQLVYSILITIW
                     YGIFGSVFAVFGLASSLAFRQKIVGVFFPCLYMILGGLFFALLDLSFLEPVGIISWGY
                     QFQLNFLLVFLHLLCIFTICLGMILYHFQFRVEDSI"
     gene            complement(1087..1497)
                     /locus_tag="AC1PNG_002045"
     CDS             complement(1087..1497)
                     /locus_tag="AC1PNG_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702503.1"
                     /translation="MKTRLSIKKPIIVAIALLGLVSPTVFSRGVLDYSMVLPRIGSTT
                     TGSIVKVTHSRGVNRNDSTGGGKTLNTSIRNYHSGSDITPTYSLPSGARVLMSYHGSG
                     SHYVNETTTLGLATQFGNTVRVQTTGSWSPDEEQ"
BASE COUNT          572 a          296 c          240 g          405 t
ORIGIN      
        1 taaaaacttc tatacaaaaa aataaaatag cagtgtagat aatcggtatc aggtagacac
       61 ctgttgttat atcttggcat agaatcagta gccaggtaaa aacatttgga acaagttgat
      121 aatgacctaa ataatattga taggtggtat ttgaaaaaat caaactccca agtaagatga
      181 taaaaaataa tctgaagata gtagattttt tatgaaaaaa tagaattcta caaaatctca
      241 tatactatcc tccactcgga actgaaaatg atataaaatc attccaagac agatggtaaa
      301 aatacacagt agatgcaaaa atacaagtag aaaatttaat tgaaactggt aaccccaaga
      361 aataatccca acaggctcta aaaaagacaa atctagcaag gcaaaaaaca acccgcccaa
      421 aatcatatag aggcaaggga aaaatacccc aacaattttt tgacgaaaag caagagaact
      481 agctaggcca aatactgcaa aaacgctacc aaaaatccca taccatatcg ttattaagat
      541 agaataaact aactgattac tatcaaaaat agacttaaaa acaccttgat agtcaccaat
      601 atatatttcc ttatatggcg tcagacatag atttgcaacc aaaagcagga aatagggtaa
      661 aaaaagggac aaaaatcctg aaattcctgc cgtcacaaga agagtccaaa tatattttat
      721 acgggatgtt ctttgtaatt gcaggcgcag aaagtgattc ttactatcct caataacccg
      781 atcagctatc ggaatcgcac tgataactgc tgccacaagg cttatcggcg aaaaacccct
      841 gatagtcgat cctgcaaaaa acagggacaa tccttcaagt ttgtatatgc cattaaaagc
      901 aaccatattc cctatcaaaa tgcaggaaaa tgccaagaga aaagacaaaa gcatcttttt
      961 agagaaaaca gttcttctaa cattcattag taaaaatttc atttttacct cctagtaaaa
     1021 agagctaggt gaataaattc cactagctca atagtctcaa taaacaactt cctagctcat
     1081 tactaatcac tgttcttcat ctggactcca actgccagtc gtttgtactc taactgtatt
     1141 tccgaattga gtagctaatc ccaaagtagt cgtttcattg acataatgac taccacttcc
     1201 atgataagac atgagaaccc tagcaccaga tggaagagaa taggtcggag ttatatcact
     1261 accgctgtgg taatttcgta ttgaggtatt taaagtcttt ccaccacctg tagaatcatt
     1321 gcgattcact cctcgcgaat gagtcacctt gactatgcta ccggttgttg tagatcctat
     1381 acgtggtaag accatggaat aatctagtac tcctcttgaa aaaaccgtcg gagaaactaa
     1441 tcccaataat gctattgcta cgataattgg cttttttata gataatctcg ttttcatgtg
     1501 aatcttctcc ttt
//
LOCUS       DBNFEG010000237         1489 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.239, whole genome shotgun sequence.
ACCESSION   DBNFEG010000237 DBNFEG010000000
VERSION     DBNFEG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.239"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            120..>1489
                     /locus_tag="AC1PNG_002046"
     CDS             120..>1489
                     /locus_tag="AC1PNG_002046"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIP6702504.1"
                     /translation="MHYISKNIWCKIRTDGRGKKENEEFMKIISFTMVNNESEIIESF
                     IRYNYNFIDEMVIIDNGCTDNTMQIIFNLIKEGYKISVYDESLEAYNQYRLDNKYLTK
                     IIAEKNPDLIIPLDADEFLTADSNPRKLLEQLDLEKIHYVNWQWFVMTKKDDINESFI
                     PRRMQYCFEKPVWHHSDGKPVTKCIISAKYYKKMNLKLSMGHHTVFGNPNVRIEHHND
                     LKFAHYRAISQEQLIYKTICYTIRDIATMENNIETAQRTNQMALIESGVDMWETAREA
                     SYSGYDCNVIHAPIDLSFCKENIVIKYNELSRETVAERVMKTGREMAVRAYNVERKQK
                     EKKFLKPIIFVLDGFKGDEYIHPNPSNHLTILTEMYNVRGLLTDNHQIKFLKVNYRLI
                     ITPDFAKFLPHEFIVVPDTLDIEQVKSQYVGTGVDLSKIISLKEYRKEIGFIGNLYAL
                     LGFVPN"
BASE COUNT          551 a          187 c          278 g          473 t
ORIGIN      
        1 tagaagataa cagaagggag ccttcaaaaa cttcattttt aattggattg tagaaaaact
       61 gttaaatcaa tatttagatt tttaggagtt cagtttttgg ggggagagct taataatcta
      121 tgcactatat ttcgaaaaat atatggtgta aaatcagaac tgatggtcgt ggcaaaaaag
      181 agaatgagga atttatgaaa attatttctt ttacaatggt taataacgaa agtgagataa
      241 tagagtcatt tatacggtat aattataact ttattgacga gatggtcatt attgataatg
      301 gttgtacaga taacacgatg caaattattt ttaatttgat taaagaggga tataaaatat
      361 ccgtatatga tgagtcttta gaggcatata atcagtatcg acttgataat aaatatctaa
      421 cgaaaataat tgctgaaaaa aatccagatt tgataatacc tttggatgcg gatgaatttt
      481 taacagccga ttcaaatcca cggaaacttt tggaacaact ggacttagaa aagatacatt
      541 atgtgaattg gcaatggttt gttatgacta aaaaagatga tattaatgaa tcgtttatac
      601 cacgtagaat gcaatattgt tttgaaaaac ctgtttggca tcattctgat ggtaaaccag
      661 ttactaaatg tataatttcc gctaagtatt acaaaaaaat gaatttaaag ctatcgatgg
      721 gacatcacac tgtttttggt aacccaaatg taaggataga acatcataat gatttgaaat
      781 ttgcacatta tcgagctatt agccaagagc aattaattta taaaacaatt tgttacacta
      841 ttcgcgatat tgctactatg gagaacaata tcgaaacagc tcaaagaaca aatcagatgg
      901 cgctcattga atctggcgtg gatatgtggg aaacggcgag agaagcctct tattcaggtt
      961 atgattgtaa tgttatacat gcaccaattg atttaagttt ttgtaaagaa aatattgtaa
     1021 taaaatataa cgaactatcc agagaaacag tagcagaacg cgtgatgaaa acgggaagag
     1081 aaatggctgt tcgtgcatat aatgtggagc gaaaacaaaa agaaaagaaa tttctaaaac
     1141 ctattatatt tgtattagat gggtttaaag gagatgagta tattcatccc aatccatcaa
     1201 atcatttgac gatcttaacc gaaatgtata acgtcagagg cttacttacc gataatcacc
     1261 aaattaaatt tctcaaagtt aattatagat taattataac tccagatttt gctaagtttt
     1321 taccgcatga atttattgtt gtaccagata ccttggatat agagcaagtt aaaagccagt
     1381 atgttggtac aggtgtagac ttgtcaaaga ttatttcttt aaaagagtat cgaaaagaga
     1441 taggctttat tggtaatttg tatgcgcttt taggatttgt tccgaatat
//
LOCUS       DBNFEG010000238         1469 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.240, whole genome shotgun sequence.
ACCESSION   DBNFEG010000238 DBNFEG010000000
VERSION     DBNFEG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.240"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            27..503
                     /gene="pezA"
                     /locus_tag="AC1PNG_002047"
     CDS             27..503
                     /gene="pezA"
                     /locus_tag="AC1PNG_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PezA"
                     /protein_id="HIP6702505.1"
                     /translation="MIGDNIKSLRKTHDLTQPEFAKIVGISRNSLSRYENGTSSLSTE
                     LIDRICQKFNVSYVDIVGEEKMLTPVEDYQLTLKIEVIKERGAAILSELYRYQDSQGI
                     TFDDETSPWILMSDDLSDLINTKIYLVNTFDEVERYNGYLDGIERMLELANHQVVA"
     gene            503..1297
                     /gene="pezT"
                     /locus_tag="AC1PNG_002048"
     CDS             503..1297
                     /gene="pezT"
                     /locus_tag="AC1PNG_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin PezT"
                     /protein_id="HIP6702506.1"
                     /translation="MRLEEFSKDDFDLALKRTIRSLTRGKTTSVTPKAILLGGQSGAG
                     KTTIHRIKQKEFQGNIIIIDGDSYRSLHPNYLTLQEEYGKDSVDYTKGFAGKMVEQLV
                     DELSKQGYHLLIEGTLRTTEVPRKTARLLKARGYQVSLALIATKPELSYLSTLIRYEE
                     VYVVNPNQARATPKEHHDGIVNHLVDNLRELENDHLFDYIQIYQRDRSCVYDSETDDG
                     SAAEVLQECLFGKWSKVEEEMMKMGQELLMEFLEMKKGRIYENKKN"
     gene            1278..>1469
                     /locus_tag="AC1PNG_002049"
     CDS             1278..>1469
                     /locus_tag="AC1PNG_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIP6702507.1"
                     /translation="MKIKKINVQNYRLLKDFSLELKSELSLIVGKNNCGKTSVLSVLE
                     KIINKSSSLTWEDINLYHRK"
BASE COUNT          517 a          215 c          317 g          420 t
ORIGIN      
        1 aagtaactaa taggaggaca ttagcaatga ttggagataa tattaaatcg ttacgtaaaa
       61 cccatgattt aacacaacca gaattcgcaa agattgttgg aatttctcga aatagtttga
      121 gccgttatga aaatgggaca agctcgttat caacagagct catagaccgt atctgtcaga
      181 aatttaatgt atcatatgtt gatattgtag gagaagaaaa aatgttaaca ccagtggaag
      241 attatcaatt aaccttaaaa attgaagtta tcaaagagcg tggcgcagcg attttgtccg
      301 aactatatcg ctatcaagat agtcaaggaa ttacttttga tgatgagact agcccttgga
      361 ttttgatgag tgatgatttg tctgatctta tcaataccaa gatttacctt gtcaatactt
      421 ttgatgaagt ggagcgttac aatggttatt tagatggtat tgagcgaatg ttggagcttg
      481 ctaatcatca ggtggtggct taatgcggtt agaagaattt agcaaagatg actttgactt
      541 agccttaaaa cgaactattc gttccttaac tcgtggtaaa acgacatcag ttaccccaaa
      601 agccatctta cttggtgggc aaagtggagc aggaaaaaca acgatacacc gtatcaagca
      661 gaaagagttc caaggtaata ttatcattat tgacggtgat agctaccgct ctttacaccc
      721 gaattatcta actttacaag aagagtacgg gaaggatagc gtggattata ccaaaggttt
      781 tgcaggaaaa atggtggaac agcttgtgga tgaactcagt aaacaaggtt atcatttgtt
      841 aatcgaagga actttgagaa cgacagaagt cccacgaaaa acagcacggc tattgaaagc
      901 gagaggttat caagtctcat tagctttgat agcaaccaag cctgaattgt cttatctcag
      961 caccttaatc cgctacgaag aagtctatgt ggttaatcca aaccaagcta gagcaactcc
     1021 taaagaacat catgatggaa ttgtcaatca cttggttgat aacttacgag agctagaaaa
     1081 cgaccatctc tttgattata ttcaaattta tcaaagagat agaagttgtg tttatgattc
     1141 ggaaactgat gacggttcag cagcagaagt gctacaagaa tgcctcttcg gaaaatggag
     1201 caaggtggag gaagagatga tgaagatggg acaggaattg ttgatggagt ttttggaaat
     1261 gaaaaaaggt agaatttatg aaaataaaaa aaattaatgt tcaaaattat cgtttattga
     1321 aggatttttc tttagagttg aagagtgaat tgtcattaat tgtgggaaaa aataattgcg
     1381 gtaaaacctc agttttatct gtattggaaa agataatcaa taaaagttct agtttaacct
     1441 gggaagatat aaatttgtat catagaaaa
//
LOCUS       DBNFEG010000239         1465 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.241, whole genome shotgun sequence.
ACCESSION   DBNFEG010000239 DBNFEG010000000
VERSION     DBNFEG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.241"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            70..738
                     /locus_tag="AC1PNG_002050"
     CDS             70..738
                     /locus_tag="AC1PNG_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HIP6702508.1"
                     /translation="MTYSQDYLDDLLVRMAYHSSGIEGNTISLPETVSIILESTLPGK
                     HKSIREFYEIENHKQAFQALLIALDNGEELSLGLVKDIHALLMDRLQHDRGQFKSVQN
                     AIIGATFKTASPEETPFLMQQWVDNANYRLGLPLEIAELLEVLADVHIQFERIHPFSD
                     GNGRTGRLILMFQSMLKLGAPVLISKEDRALYMELLAEQDVTGLSQLLKRSLEFEQER
                     KKQF"
     gene            837..>1465
                     /locus_tag="AC1PNG_002051"
     CDS             837..>1465
                     /locus_tag="AC1PNG_002051"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIP6702509.1"
                     /translation="MGEVADFSIKTNSLSRDKLSSYFYEVQNIHYGDILTKYDAILDV
                     CNKELPSIIGSTISDFADALLSEGDIVFADAAEDSTVGKAIEVRNFKGKNVVSGLHTI
                     VARPKVSYAPYYLGYLINSTAYHNQILPLMQGTKVSSISKANLKSTTVVFPTLPEQEA
                     IGSFFSDLDQLITLHQRKLDDVKELKKALLQKMFPKGNGNDFPELRFPE"
BASE COUNT          441 a          262 c          330 g          432 t
ORIGIN      
        1 cagttggatc atctgaaact gttgaaaaaa gcattgttgc agcagatgtt tatatagaaa
       61 gaggtcagta tgacatattc gcaggattat ttagatgacc ttctggtacg aatggcctat
      121 cattccagcg ggattgaggg gaataccatt tctctgcctg agacggtttc gattattttg
      181 gaaagtacgc tacctggcaa gcacaagagt atccgtgaat tttacgagat tgaaaatcac
      241 aaacaagcct tccaagctct gctaattgcc ttagataatg gggaagagct gagtttggga
      301 ctggttaagg atattcatgc cttgttgatg gaccgcctcc agcatgatcg ggggcagttt
      361 aagtcggtac aaaacgctat tattggtgcg accttcaaga ctgcatcgcc agaggaaacg
      421 ccttttctca tgcaacagtg ggtggataat gccaactatc gcttgggttt gcctttggaa
      481 atagctgagc tgttagaggt gctggcagat gtgcatattc agttcgagcg tattcaccca
      541 tttagtgatg ggaacggccg gacaggacgg ctgattctga tgtttcaaag tatgttaaag
      601 ctgggagcgc ccgtactgat tagtaaagaa gatcgagctc tttatatgga gttgttagcg
      661 gaacaagatg tgactggatt atcccaatta ctcaaacgct ccttggagtt tgaacaagaa
      721 cgaaaaaaac agttttaagt cgagttttca gaaagcgact ttcaatcgga actattattt
      781 ttgcctgaaa atacaatgaa aaccttactt tcacaactac ttggaaacag cgtaagttgg
      841 gggaggtggc ggacttttcc ataaaaacaa actcactttc tagagataag ttatcatcgt
      901 atttttatga agttcagaac attcattatg gggatatttt aacaaaatat gatgctattt
      961 tagatgtatg taacaaagaa cttccatcaa ttattggaag tacgatttca gactttgcag
     1021 atgctttact tagtgaagga gatattgttt tcgcggatgc agcagaagac tcgactgttg
     1081 ggaaagctat tgaagttcga aattttaagg gtaagaatgt tgtttctggt ttacatacga
     1141 tagttgctag gccgaaagtt tcttatgccc cctactattt aggttatcta attaattcaa
     1201 ctgcatacca taatcaaatt ttacctttaa tgcaggggac aaaagtgagc tcaattagta
     1261 aggctaattt aaaatccacg acagtagtgt tccccaccct ccctgaacaa gaagccatcg
     1321 gcagcttctt ctccgaccta gatcagctta ttactcttca tcagcgaaaa ttagatgatg
     1381 ttaaagaatt gaagaaggct ttgttacaga aaatgtttcc gaaagggaat ggaaacgatt
     1441 ttcctgagct aagattccca gaatt
//
LOCUS       DBNFEG010000240         1460 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.242, whole genome shotgun sequence.
ACCESSION   DBNFEG010000240 DBNFEG010000000
VERSION     DBNFEG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.242"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..526)
                     /locus_tag="AC1PNG_002052"
     CDS             complement(<1..526)
                     /locus_tag="AC1PNG_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIP6702510.1"
                     /translation="MTQIKWRDCLLICFGAILYALVLNMLIIPHEFGEGGITGVTLLL
                     FYTTGIETSLSSFVLNGILIVLAYRFLSKLTVFYTFLAVATMSLTMHYTTFLQIKWLP
                     LIPSALLAGLVTGLSMALVIYGNGSTAGSDIIALLCNKYFKWKISTVILIFDVFVVTP
                     LAFKIGLVKTFWTLV"
     gene            complement(604..987)
                     /locus_tag="AC1PNG_002053"
     CDS             complement(604..987)
                     /locus_tag="AC1PNG_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIP6702511.1"
                     /translation="MGLFSGLLGNASQMDNDKIEQELEHVLLDNEQVEMAFSLVRDLI
                     VFTEYRLILVDKQGMTGKKVSYKSIPYRSISRFTVETSGHFDLDAELKIWISSAVEPA
                     EILQFKSDKSVIAIQKALASAVLGK"
     gene            complement(1075..>1460)
                     /locus_tag="AC1PNG_002054"
     CDS             complement(1075..>1460)
                     /locus_tag="AC1PNG_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine triad protein"
                     /protein_id="HIP6702512.1"
                     /translation="IEENEKLVNLDVEGNQIKTLEIEGKQESVVRLNVADNQLKNLEG
                     INDYKALEDLNASKNDIETLAITEPNKTLKTIDVSEDHIPKEELNLNDQKIPSAIAEH
                     FPAVEGGSIENNQPKEVDKEAKVGE"
BASE COUNT          470 a          324 c          213 g          453 t
ORIGIN      
        1 ttaccaatgt ccaaaatgtt tttacaagcc caattttaaa agccaaagga gttaccacaa
       61 atacatcaaa aatgagaata acggtggaaa tcttccattt gaaatattta ttacacaaca
      121 atgcaataat atcacttcct gccgtggacc cgtttccata aatgactaga gccattgata
      181 aacctgtcac caatcccgcc aaaagagcag aaggaatcag gggcaaccat tttatttgca
      241 gaaaagtcgt ataatgcatg gttaaggaca tagtagcaac cgctaagaaa gtatagaaaa
      301 ccgttaactt actcaaaaag cgataggcta atacaatcaa aatcccattc aaaacaaaac
      361 tggacagact tgtttcaatc cctgtcgtat aaaacaaaag tagggtaaca ccagtaatcc
      421 caccttcgcc aaattcatgt ggaatgatga gcatattcaa aacaagggca tataggattg
      481 ccccaaaaca tatcaataag cagtctctcc attttatctg cgtcatattc tctcctcaca
      541 aacagtccac cttagtatat caaaaaaagc agaaagcgga tagccacttt ctgtactttt
      601 ttcctatttt cctaagacag ctgaagccag tgctttttga atggcaatga cactcttatc
      661 gctcttaaac tgaagaattt cagcaggttc aacagccgat gaaatccata ttttcagctc
      721 agcatctaag tcaaaatgac cagaggtttc taccgtaaaa cgtgaaatcg agcggtaagg
      781 aatcgattta taagatacct ttttcccagt cattccttgc ttatcgacca aaatcaatcg
      841 atattctgta aaaacaatca gatcacgaac cagactaaat gccatctcaa cctgttcatt
      901 atccagtaga acatgctcca attcctgttc aatcttatca ttatccattt gcgaggcatt
      961 acctagtaaa cctgaaaata gtcccataat atgactcctt tggtattttt ctatagtata
     1021 acacaaaaag ctactagttg actatcttag gtaggtcttt accaaacatg agaattactc
     1081 gcctaccttt gcttccttat caacttcttt aggttgattg ttctcgatac tgccaccttc
     1141 aactgctgga aaatgttctg caatcgcaga tgggatcttc tgatcattta gatttaattc
     1201 ttccttcgga atgtgatctt cactcacatc aattgttttc aaagtcttat ttggctccgt
     1261 gatggcaagt gtttcaatat cattttttga agcattcagg tcttctaagg ctttgtaatc
     1321 attgattcct tctaaattct tcaattgatt atctgcaaca tttaatcgaa caaccgattc
     1381 ttgtttaccc tctatttcca aagtcttaat ttgattcccc tctacatcca gatttactaa
     1441 tttttcattt tcttctatcc
//
LOCUS       DBNFEG010000241         1459 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.243, whole genome shotgun sequence.
ACCESSION   DBNFEG010000241 DBNFEG010000000
VERSION     DBNFEG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.243"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..642)
                     /locus_tag="AC1PNG_002055"
     CDS             complement(<1..642)
                     /locus_tag="AC1PNG_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702513.1"
                     /translation="MDVFPSFFIFLFNKLNIFVDLKNIFCYTCVMKTSTLGKLLELPN
                     FHHFYTVPIRENTESSGNYIIVFEQDKQPVFVDLKNETGHTREVRFASHFDSAGLLVG
                     LGAIPINYWPTCQILGVQKNNNRRGLSLSSLMDGYGRLPIFKEMAERYRARGQSTIYD
                     PYRYSYTIDEKKVIKDNQLTLIPKKEYEKIKDFHPFLQTKHGNVYAVPCFYQDR"
     gene            complement(670..1458)
                     /locus_tag="AC1PNG_002056"
     CDS             complement(670..1458)
                     /locus_tag="AC1PNG_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702514.1"
                     /translation="MAVLKYSKARLLVLLIATGLSCIGIYWLGKEQNRLLNEQCHALN
                     IRIINDLGAKIDAIGGPQNPRIIGFFQQDDTTAISQRIGTASEEELKIAKPDNLFQKE
                     WIVLYPQTRSSPFENTSAYAVMKTSIKADWLHVTTSSETELDIFYEKADESLLTLEDL
                     VQDKESFHATLKTILVSAKNEAEIQVQKDILEMFESDDWSAIPFAYTEKSLILEKAVI
                     SVSAFVDSLNPYYFSEQTLADLRLSEESRQALEDSVDKTIITYP"
BASE COUNT          450 a          294 c          265 g          450 t
ORIGIN      
        1 tctgtcttga tagaaacaag gaactgcata aacatttcca tgctttgttt gcaaaaaagg
       61 atgaaaatct tttatctttt cgtattcttt ctttggaata agggttaatt gattatcctt
      121 aataaccttc ttttcatcaa tagtatatga ataacggtag gggtcataaa tggttgattg
      181 tccacgcgct ctataacgct ctgccatttc cttaaaaata ggtagacgtc cataaccatc
      241 catcagacta gatagactaa gtcctcgtcg attattattt ttctgcacac caagaatctg
      301 gcaggtaggc caataattga taggaatggc tcctaagccg accagaagac cggcgctgtc
      361 aaaatgactg gcaaagcgta cctcacgagt gtgaccagtc tcatttttca aatcaacaaa
      421 tacaggttgc ttgtcctgtt caaaaacaat tatataattt ccagaagact ccgtattttc
      481 tctaattgga acggtgtaga agtggtgaaa atttggtaat tctagtaact ttccaagtgt
      541 tgatgttttc ataacacaag tataacaaaa aatatttttc aaatccacaa aaatgtttaa
      601 tttgttaaac aaaaatataa agaagctggg aaagacatcc atgacgactt tcccactccc
      661 accgtttatt caaggatagg tgataattgt cttatctacc gaatcttcta aagcctggcg
      721 cgattcttct gataaacgca aatctgcaag ggtttgttct gaaaaatagt agggatttag
      781 actatctaca aatgcagaaa ctgaaatgac agctttctcc aatatcagag atttttcagt
      841 gtaagcaaag ggaatagcag accaatcatc actctcaaac atttctaaaa tatctttttg
      901 aacttgaatt tcagcctcat ttttagctga aactaggatt gtttttagtg tagcatgaaa
      961 actttccttg tcttgcacca agtcttccaa ggtcagcaag ctttcatctg ccttctcata
     1021 aaaaatatct aattccgtct ctgacgaagt agtcacatgg agccagtcag ccttgatact
     1081 ggttttcatt actgcatagg cagaagtatt ttcaaaggga ctgctgcgcg tttgaggata
     1141 gagaacaatc cactctttct ggaatagatt gtcaggttta gctattttca gttcctcctc
     1201 agaagcggta ccaatccgct ggctgatagc agtagtgtca tcttgctgaa agaatccaat
     1261 gatacgcgga ttttgcggac cgccaatggc atcaattttt gctcccagat cattaataat
     1321 gcgtatgttc aaggcatgac actgctcatt taatagtcta ttttgctctt ttccgagcca
     1381 gtagattccg atacaagata gaccagtggc aataagcaag acaagtaacc gcgctttcga
     1441 gtatttcaac acagccatc
//
LOCUS       DBNFEG010000242         1442 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.244, whole genome shotgun sequence.
ACCESSION   DBNFEG010000242 DBNFEG010000000
VERSION     DBNFEG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.244"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1334
                     /locus_tag="AC1PNG_002057"
     CDS             <1..1334
                     /locus_tag="AC1PNG_002057"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent nuclease"
                     /protein_id="HIP6702515.1"
                     /translation="DNATKNLLQSAITEADEALTKAYSGDGEDDGVFTSIFERVKKFG
                     GNDSESELEIHSSLSEKDILSNNTTLYYRHDDSLLPETYNGLGYLNLYGMIFEIETLM
                     ADIKNNPADINLVYIEEPESHTHPQLQYVFIKNIKGLLKEHDDELKASGYTSGIQTLI
                     TTHSSHIVSDCNFDDLIYFKHDNGTAISKAFNSLKEEYEDDKLGYRFVKQYLTLNSSE
                     LFFADKVICIEGDTERILLPTMMQKVDIATPIEQGSNIMPLLSQNISIIETGAHSQVF
                     RKFFDFLGIKVLIITDIDPVNKNEKNRFIACSAVNATSTSNTSLKSFFNISGDGIFST
                     VTQKSFAEKITPDNRIRIAYQIPEDNNGYQPASFEDSFIGLNKEFIIKQKDGLIEYEA
                     LKNFDDDEIEDFYTFARNKVNKKSAFASSLLYFEDEENTWKVPKYISEGLL"
BASE COUNT          537 a          188 c          259 g          458 t
ORIGIN      
        1 ttgacaatgc tacaaaaaat ttacttcaat ctgcaattac agaggcagat gaagctttaa
       61 ccaaggcata tagtggtgat ggtgaagacg atggggtttt cacctcgatc tttgaaagag
      121 taaaaaaatt tggtggaaat gatagtgaat cagagttgga aattcattca tctctttctg
      181 agaaagacat tttgagtaat aatacaacac tatactatag gcatgacgat agtttattac
      241 cagaaacata caatggtttg ggatatttaa atctatatgg gatgattttt gaaatagaga
      301 cgttgatggc agatatcaaa aataatcctg ctgatattaa tttagtttat attgaggagc
      361 cagagtcgca tacacatcca caattgcaat atgtttttat caagaatatt aaaggtttgc
      421 tgaaggaaca tgatgatgaa ctaaaagcaa gtggatatac tagtggtatt caaactttaa
      481 ttacaactca ttcatcacac atagtgtctg attgcaactt tgatgattta atttatttta
      541 agcatgataa tggcacagct atatcaaagg cttttaattc attgaaagaa gaatatgaag
      601 atgataaact tgggtacaga tttgttaagc agtatttgac actcaatagt agtgaactat
      661 tttttgctga taaagtcatt tgtattgaag gtgacacgga aagaatttta ctgccaacaa
      721 tgatgcaaaa agtggatata gcaactccaa tagaacaagg ttcaaatata atgccattac
      781 tttctcaaaa catttcaatc attgaaacag gtgctcattc acaagtgttt aggaaatttt
      841 ttgacttttt agggataaaa gtattgataa ttactgatat tgatcctgta aataaaaatg
      901 aaaagaatcg ttttatcgct tgttcagcag taaatgctac atctacgtca aatacttctc
      961 ttaaaagttt cttcaatatt tcaggagatg gaattttttc aacagtaact caaaaaagtt
     1021 ttgctgaaaa aataacacca gataatagaa tccgtattgc atatcaaatt cccgaagata
     1081 acaatggtta tcagcctgct agttttgaag attcctttat cggtttaaat aaagaattca
     1141 ttattaaaca aaaagatggt cttattgaat atgaagcttt aaaaaacttt gatgacgatg
     1201 aaatagaaga tttctataca tttgctagga ataaagtgaa caaaaagtct gcctttgcat
     1261 caagtttatt atactttgaa gatgaagaaa atacttggaa agtacctaaa tatatttcgg
     1321 agggattatt atagttacga gaacagtaga gcaagaagta gaaaaagtat ttgaagcaat
     1381 aaaacaaggt caaaatttta ttcttgaagg cggtgctgga agtggtaaaa cttattcgct
     1441 aa
//
LOCUS       DBNFEG010000243         1441 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.245, whole genome shotgun sequence.
ACCESSION   DBNFEG010000243 DBNFEG010000000
VERSION     DBNFEG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.245"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(128..1420)
                     /gene="purB"
                     /locus_tag="AC1PNG_002058"
     CDS             complement(128..1420)
                     /gene="purB"
                     /locus_tag="AC1PNG_002058"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIP6702516.1"
                     /translation="MIERYTRPEMGNIWSEENKYKAWLEVEILADEAWAELGEIPKED
                     VALIREKATFDIDRILEIEEETRHDVVAFTRAVSESLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAREHKYTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNMERFDVAAKGVEAGKISGAVGNFANIPPFVEEYVCGKLGIRPQEISTQVLPRDL
                     HAEYFSALALIATSIERMATEIRGLQKSEQREVEEYFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVVRGHLVTAFENVALWHERDISHSSAERIITPDTTILINYMLNRFGNIVKNL
                     TVFPENMKRNMESTFGLIYSQRVMLSLIEKGMTREEAYDLVQPKTAQSWDNQVDFKPL
                     LEADERVTAKLSQEEIDELFNPDYYAKRVDDIFERLGL"
BASE COUNT          365 a          366 c          307 g          403 t
ORIGIN      
        1 tttgcaaaaa ataatttatt tttacttacg aatgattgta tagcggagaa agcttgtttt
       61 ccccttcaac tatttattcg caagtaaata aaaaaattct ctggaaaatc caaagaattt
      121 tgatttttta taatccaagt cgttcaaaga tgtcatcaac ccgcttagca tagtagtctg
      181 ggttgaagag ttcatcgatt tcttcctggc taagtttggc agtcacacgc tcatccgctt
      241 cgagaagggg cttgaagtcc acttgattat cccatgactg cgcagttttt ggttgcacca
      301 aatcataggc ttcctcacgg gtcatgcctt tttcaatcaa actgagcatg acacgctggc
      361 tgtaaatcaa gccaaaggtt gactccatat tgcgtttcat attttcaggg aagaccgtca
      421 agttcttaac gatattgcca aaacggttga gcatataatt gatgaggata gtcgtatccg
      481 gcgtgataat ccgctccgct gacgagtggg aaatatcacg ttcgtgccag agagccacat
      541 tctcaaaagc cgtcaccaag tgaccacgca ccacacgagc cagaccggtc atattttcag
      601 agccgatcgg gttgcgttta tggggcatag cagagctgcc tttttggcct ttggcgaaat
      661 actcttcgac ttcacgttgt tcagattttt gtagtccacg gatttctgtc gccatacgct
      721 caatagacgt tgcaatcaag gctagggctg agaaatattc tgcgtggagg tcacgaggta
      781 ggacctgggt agaaatttcc tgcggacgaa tgcccaattt accacaaaca tactcttcca
      841 caaatggagg aatgttggca aagtttccaa ccgctcctga aattttacca gcctcaacgc
      901 ccttagccgc cacatcaaaa cgctccatat tgcgtttcat ttcgctatac caagtcgcca
      961 acttcagacc gaaggtcgtt ggctccgcat ggacaccgtg ggtccgcccc atcataatgg
     1021 tgtacttgtg ctcacgcgcc ttgtcggcga taatgttggt aaagttttca aggtcacgac
     1081 ggatgatgtc gttggcctgc ttatagaggt agccgtaagc cgtatccacc acgtcggtcg
     1141 aagtcagacc gtagtggacc cacttgcgtt cctcaccaag actttccgaa accgcacgcg
     1201 taaaagccac cacatcgtga cgggtttcct cttcaatctc taaaatgcgg tcgatgtcaa
     1261 aagtcgcctt ctcacgaatc aaggccacat cttctttggg aatctcaccc aactcagccc
     1321 aagcctcatc agcgaggatt tccacctcca accaagcctt gtacttgttc tcttcactcc
     1381 aaatattgcc catttcaggg cgtgtgtagc gttctatcat gttgtgtttc ctttttccct
     1441 a
//
LOCUS       DBNFEG010000244         1420 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.246, whole genome shotgun sequence.
ACCESSION   DBNFEG010000244 DBNFEG010000000
VERSION     DBNFEG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.246"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            307..1188
                     /locus_tag="AC1PNG_002059"
     CDS             307..1188
                     /locus_tag="AC1PNG_002059"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIP6702517.1"
                     /translation="MPLVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKQAP
                     VLIQTSMGAAKYMGGYKVARNLIANLIESMNITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLAKAKEVVELAHAKGISVEAEVGTIGGEEDGIVGAGELAPIEDAV
                     AMVATGIDFLAAGIGNIHGPYPANWEGLNLDHLRKLTEAVPGFPIVLHGGSGIPDAQI
                     QEAIKLGVAKVNVNTECQIAFANATRKFAAAYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSANKA"
BASE COUNT          462 a          264 c          314 g          380 t
ORIGIN      
        1 attggcagac gcgcaggact aaggatcctg tgaccgcttt aggtcgtgtg ggttcaagtc
       61 ccactctccg catagtggac tagctaaggc tagtcctttt gctttgtcta gataagtgca
      121 gagcacgata tatgctagtt cataaattac aagaatgata cgaaacaggc attattatgg
      181 ctttcatgaa aagatatgtg aaagttttct caaaactaga cggtttcaac tagcattttc
      241 aaaataaaaa tggtagaata gaagagtatg aggtgcaacc tcaaaaaata ttaggaggct
      301 atcgaaatgc cattagtttc agcagaaaaa tttgtccaag cagcgcgtga caacggttat
      361 gcagttggtg gatttaacac aaacaacctt gagtggactc aagctatctt gcgtgcagca
      421 gaagcaaaac aagctccagt tcttatccaa acttctatgg gtgcagctaa gtacatgggt
      481 ggttacaaag tagctcgtaa cttgattgca aacttgattg aatcaatgaa cattacagtt
      541 ccagttgcta ttcaccttga ccacggtcac tacgaagatg cacttgagtg tatcgaagtt
      601 ggttacactt ctatcatgtt tgacggttca caccttccag tagaagaaaa ccttgcaaaa
      661 gcaaaagaag ttgtagaatt ggcgcacgct aaaggtatct cagttgaagc tgaagttggt
      721 actatcggtg gtgaagaaga tggtatcgta ggtgctggtg aattggctcc aattgaagac
      781 gcagtagcaa tggttgcaac tggtattgac ttcttggcag caggtatcgg taacatccac
      841 ggtccatacc cagcaaactg ggaaggtctt aaccttgacc acttgcgtaa attgactgaa
      901 gctgtaccag gtttcccaat cgtattgcac ggtggttcag gtattccaga tgcacaaatc
      961 caagaagcaa tcaaattggg tgttgctaaa gttaacgtaa acaccgagtg ccaaatcgca
     1021 tttgcaaacg caactcgtaa gtttgcagca gcttatgaag caaacgaagc agaatacgat
     1081 aagaaaaaac tctttgaccc acgtaaattc ttggcagacg gcgtgaaagc tatccaagca
     1141 tctgttgaag agcgtatcga cgtattcggt tcagcgaaca aagcttaatg taactgcgaa
     1201 gaaatctcag atttcaagca aacaaagcat aagtttctta gattaaacca aaaaggattt
     1261 tctttcgaga atccttttta ttataaatag ttgattaagt ttgatctagt tctttgccat
     1321 gaagaaatct acgatagaag tgagcagaga acagttaaaa tataaaacgc tccaaagttt
     1381 tcatgactgt aataacttag agaacgaaga attatggatg
//
LOCUS       DBNFEG010000245         1407 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.247, whole genome shotgun sequence.
ACCESSION   DBNFEG010000245 DBNFEG010000000
VERSION     DBNFEG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.247"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(24..443)
                     /locus_tag="AC1PNG_002060"
     CDS             complement(24..443)
                     /locus_tag="AC1PNG_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIP6702518.1"
                     /translation="MELKDIMHILEDMKVGVFATLDEYGNPHARHAHITAANEEGIFF
                     MTSPETHFYDQLMGDQRVAMTAISEEGYLIQVVRVEGTARPVENDYLKTVFADNPYYQ
                     HIYKDESSDTMQVFQIYAGHGFYHSLPQGHKYIFSIG"
     gene            complement(994..>1407)
                     /locus_tag="AC1PNG_002061"
     CDS             complement(994..>1407)
                     /locus_tag="AC1PNG_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702519.1"
                     /translation="SDMFPKNMNDAELLRFIIYGYFSQAMDVFLTSYTFNAFPFFSSL
                     TTYLNYNVLLLNIVHYSKQENLEHFLKMSKLTLPIWFQYNQKGVELSISEISERAKNI
                     NFSDRIREIYNALDDFNTQEQLITEIENCVHLLIK"
BASE COUNT          473 a          265 c          211 g          458 t
ORIGIN      
        1 gcacgaactt ctgaatcctc accttaaccg atagaaaaaa tatatttgtg cccttgaggc
       61 aaactatgat agaaaccatg acctgcataa atctgaaata cttgcattgt atcactagac
      121 tcatctttat agatatgttg ataataagga ttatccgcaa aaactgtctt tagatagtcg
      181 ttctccacag gtctggcagt cccttctaca cgcactacct ggataaggta cccttcctca
      241 gaaatagccg tcatcgcaac tctttgatcc cccatcaatt gatcatagaa atgcgtttct
      301 gggcttgtca taaagaaaat cccttcttcg tttgctgcag tgatatgagc atgacgagca
      361 tgtggatttc catactcatc cagagtagca aaaactccaa ccttcatatc ttccaaaata
      421 tgcataatat cttttagttc catggtatca ccccttgaaa atacctattt tctaaagtat
      481 atcaaaaaca agcgaaaatg ttaagtggaa tattgataga aatggtgaaa gtgtttcatg
      541 aataactccc tactgacgga accatttaag aaggcagcta tgtttgcttc attttcaacc
      601 ttcaaaggtt cctcaaccct tttgatttcc aattggtatg gatttgacta ctgaaccgat
      661 tatgacaaca atcaaaatcc gtcgttacta taaaaactac ctaattataa aaaagcgact
      721 cttgagtgct ttgaaacgtt gatatttcgc ggtatattaa cgtttactaa actggcttgc
      781 tttacgtgct ttcttaagac ctggttttgg gtgacagaag taaaatgcct ataaaagatt
      841 attaaatcaa gctttcaaag tccattttca tcacatttaa ccacttataa ctcaatcata
      901 caaacaatgg attaccatta gattaccaaa aatctgtttt cggagcttat gtgattgagg
      961 aacaacatta ctgacaatct ctatcttcac aatttacttt attaataaat gtacacaatt
     1021 ctcaatctca gtaatcaact gctcttgggt gttgaaatca tcgagagcat tataaatctc
     1081 tcttatacga tctgaaaagt ttatattttt tgctctttca cttatttcag aaatacttag
     1141 ctcaactcct ttttgattgt attgaaacca aattggtaaa gtcaattttg acatttttaa
     1201 gaaatgctct aaattttctt gtttagaata atgcacgata tttaacaaaa gaacattata
     1261 atttaaataa gtagttaagc tagaaaaaaa tgggaaagcg ttgaaagtgt aagaagtcag
     1321 aaaaacatcc atagcttgac tgaaatatcc gtagattata aatcgtaaaa gttctgcatc
     1381 attcatattt ttagggaaca tatctga
//
LOCUS       DBNFEG010000246         1396 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.248, whole genome shotgun sequence.
ACCESSION   DBNFEG010000246 DBNFEG010000000
VERSION     DBNFEG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.248"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..604
                     /locus_tag="AC1PNG_002062"
     CDS             <1..604
                     /locus_tag="AC1PNG_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HIP6702520.1"
                     /translation="GYMKISKVIFRDVVYLKKLGLNGYISCQELRAGFPHNFPNFVMG
                     QMLWNRNLEYDELKALYFSSLYGSEWKSVVTYLEKLSDYSSCDYFNSIGDRQNAELSM
                     KYNISSKLAFNFINILEENVAKNYGIKRDAWRQLGYHREYVIKLANALYLMASGDYLD
                     GQIQWRDFLDYIRNHESDFQPYLDVYRIIEVAKNYAGFKL"
     gene            724..>1396
                     /locus_tag="AC1PNG_002063"
     CDS             724..>1396
                     /locus_tag="AC1PNG_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIP6702521.1"
                     /translation="MNKKIFLNSAIILLSVTCFTGSVDALDNSRVIAIPSEIVNSENA
                     YNITLKAKTDGVESDLGGSIVSENGVLSGSNGTKINLKITTNPGYRFVGYQSFLENGN
                     STDGLLPIENDSFEISDKTGNVTVIAIFESVPVDESIYFSDDFSVENINNYVISENLV
                     GKIGISDGKMNLHAPGISSIKPILTKPIEDNATSGYEIGFTLQQIGEVKPWNTFRIVF
                     KEDHDG"
BASE COUNT          489 a          178 c          262 g          467 t
ORIGIN      
        1 aggatatatg aagatcagta aagtaatctt tagagatgtc gtatatctta aaaaattggg
       61 tttaaatggt tatattagtt gccaagagtt acgcgcaggt ttccctcata acttccctaa
      121 ctttgtgatg ggtcaaatgt tgtggaacag aaatttagag tacgatgaat tgaaagcgct
      181 ctatttcagt tccttatatg gttctgaatg gaaaagtgtt gtaacgtact tagaaaaatt
      241 atcagattac tcttcatgtg attattttaa ttcaataggt gatcgccaaa atgctgaatt
      301 atcaatgaaa tacaatatct caagtaaatt agcatttaat tttataaata tcttggaaga
      361 gaatgtggct aaaaattacg gaattaaacg tgatgcttgg cgacaattag gttatcaccg
      421 agaatatgtt atcaaattgg ccaacgccct ttatctaatg gcctctggag actatctcga
      481 tggccaaatc caatggagag actttttaga ttatattaga aatcatgaat cagattttca
      541 accatatctt gatgtttacc gtatcattga agttgctaaa aattatgctg gattcaaatt
      601 gtagtcctat ggattagatt agaaaaatag ccaattcact atttattttt aagtgaattg
      661 gcttgatgtc tattattaga aggataaata aaaatatttg atgttatttt ttgagagggt
      721 tacatgaata aaaaaatttt tttaaactca gcgattatat tactctctgt tacttgtttt
      781 actggttctg ttgatgcttt agataatagt agagtgatag cgataccaag cgaaatcgta
      841 aacagcgaaa acgcatacaa cattacttta aaagcgaaaa ctgatggagt tgaaagtgat
      901 cttggaggaa gtattgtttc tgaaaatggt gtcttatcag gttctaatgg cactaaaata
      961 aatttgaaga taaccacaaa tcctggttat cgatttgtag gttatcaatc atttttagaa
     1021 aatggaaata gtacggatgg attgctacct atcgaaaatg atagttttga aatttcagat
     1081 aaaacaggca atgtgaccgt aatagcaata tttgagtcag ttccagttga tgaatcaata
     1141 tattttagtg atgatttttc agttgaaaat attaataatt atgtgatatc tgaaaacctg
     1201 gtaggaaaaa ttggaattag tgatgggaaa atgaatcttc acgctccagg tatctcttcg
     1261 atcaaaccaa ttttaacaaa accaatcgaa gataatgcaa cttctggata tgaaattggg
     1321 tttacattac aacaaattgg tgaagtaaag ccatggaata cttttagaat agtatttaaa
     1381 gaagatcatg atgggt
//
LOCUS       DBNFEG010000247         1378 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.249, whole genome shotgun sequence.
ACCESSION   DBNFEG010000247 DBNFEG010000000
VERSION     DBNFEG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.249"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            82..672
                     /locus_tag="AC1PNG_002064"
     CDS             82..672
                     /locus_tag="AC1PNG_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HIP6702522.1"
                     /translation="MSKKEILLEFIENHNGIITYKDCKALKIPTIYLTRLEKEGIIYR
                     VEKGIFLTQNGDYDEYYFFQYRYPKAVFSYISALYLQQFTDEIPQYFDVTIPRGYRFN
                     TPPANLNIHSVSKEYSELGITLVKTPMGNEVRVYDFERIICDFVIHREKIDTELFVKT
                     LQHYGNYSKKNLTKLYEYATKMNTLDKVKQTLEVLV"
     gene            669..>1378
                     /locus_tag="AC1PNG_002065"
     CDS             669..>1378
                     /locus_tag="AC1PNG_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HIP6702523.1"
                     /translation="MNKAKLTALCHKISKNTGLTFNSVMTYYFLEVILKKLSQSTYSN
                     HYIFKGGFLLSNVIGVESRSTVDIDFLFHQMTLSEDTVKQQLKEILADSEEGISFVIQ
                     SITAIKESDDYGGYRATISCQLENIKQVIHLDIATGDVVTPQPITYDYKAIFDEENFP
                     IIAYTIETILAEKLQTIYSRNFLNSRSKDFYDVYILSKLKKEDIDFIQLKNACQRTFS
                     YRETELDFVKIIELLERF"
BASE COUNT          494 a          247 c          225 g          412 t
ORIGIN      
        1 attgttgtaa aagaaaacgt tgcttttgca ctacaaatat tgtaaaattg tagtgcaaaa
       61 gcaacattaa aaggagtaag catgtcaaaa aaagagattc tacttgaatt tatagaaaac
      121 cacaatggca ttatcaccta taaagactgt aaagccttaa agattcctac aatatatttg
      181 acaagactag aaaaagaagg gattatttac cgcgtcgaaa aaggaatttt cctaacccag
      241 aatggagact acgacgaata ctacttcttt cagtaccgtt atcccaaggc tgttttctct
      301 tatatctcag cgctttacct gcagcaattt acagatgaaa ttcctcaata ttttgacgta
      361 accattccta gaggctaccg ttttaatact cctccagcaa atctgaacat tcattccgtt
      421 tctaaggaat atagcgaact aggaattaca cttgtaaaaa cccctatggg aaacgaagta
      481 agagtgtatg attttgaacg cattatctgt gattttgtca ttcatcgaga aaaaatagat
      541 actgagcttt ttgtcaaaac acttcaacat tacggaaact attctaaaaa aaatcttaca
      601 aaactctatg aatatgcgac aaaaatgaac accttggaca aggtcaaaca aactctggag
      661 gtcctagtat gaataaagct aagctaacag cactctgtca taaaatatca aagaatactg
      721 gtttaacctt taattccgtc atgacctatt acttcctaga agtcattttg aaaaaattaa
      781 gtcagagtac ctactcaaat cactatatct tcaaaggggg gtttctgctg tcaaatgtta
      841 tcggagttga atctcgtagc acggttgata ttgatttttt gttccatcaa atgacactct
      901 cagaagatac tgtgaagcag caactcaagg aaattttagc agattccgaa gaaggtatat
      961 cttttgtgat tcaatcaatc acagctatta aagaaagtga tgactatggt ggctatcgag
     1021 caacaatttc gtgccaactt gagaatatta aacaagttat ccatttggat attgcgacag
     1081 gtgatgttgt aactcctcag ccgattacat acgactataa agctattttt gatgaagaga
     1141 attttccaat cattgcttat acaattgaaa caattctagc tgaaaaactt caaaccatct
     1201 attcacgtaa cttcttgaac agtagaagca aagattttta cgatgtttat attctttcaa
     1261 aactgaaaaa agaagacatt gacttcattc agttgaaaaa tgcctgtcag agaacatttt
     1321 catatcgcga aacagaactc gattttgtaa agattattga actactcgag aggttcaa
//
LOCUS       DBNFEG010000248         1357 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.250, whole genome shotgun sequence.
ACCESSION   DBNFEG010000248 DBNFEG010000000
VERSION     DBNFEG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.250"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..778
                     /locus_tag="AC1PNG_002066"
     CDS             <1..778
                     /locus_tag="AC1PNG_002066"
                     /inference="COORDINATES: protein motif:HMM:NF012738.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6702524.1"
                     /translation="NYGRIKMKSNKEQKYFWLTFSLIVALMIWWFLSNYDRTSRSFPN
                     IILVWNALLIMIDKGFIFEDIFSSLTSVFFGFTSGFLLSVPNALFMSWYSPYRNIVYP
                     WIQYIRNIPPLAYVPLVVISAGVGLLPQVIVIAIATFLTMTVTIYQGIINIDPNLIKA
                     ARILGASDFEIFKRILVPASIPFIMTAVRLGTSISLTTIIAAESTGATSGMGMRIRSL
                     NNSFDSSGMLLYIILIGVIGLLLNKLVDLIERKLTGWQQK"
     gene            763..>1357
                     /locus_tag="AC1PNG_002067"
     CDS             763..>1357
                     /locus_tag="AC1PNG_002067"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6702525.1"
                     /translation="MATEVKKIKVKIEEVSKSFQSIHGEVVALNQINLEIFENEIVTV
                     VGPSGCGKSTLLNLVAGLEPVTSGKIYCDDNEIVGVGVERGVVFQQYALFPWMSVQQN
                     VMFGPEMQGKSKLEASEIANKYIKMVGLQEFSNHYPKELSGGMKQRVAIARAYAVNPT
                     ILLMDEPFGALDAQTRIQLQDELIEMLEHERKTCLFIT"
BASE COUNT          430 a          192 c          274 g          461 t
ORIGIN      
        1 taactatggg aggataaaga tgaagagcaa caaagaacaa aaatattttt ggttgacatt
       61 ctcattgatt gttgcattga tgatttggtg gtttttatcg aattatgatc gaacctctcg
      121 tagttttcca aatatcattt tggtttggaa tgctttatta atcatgatag acaaagggtt
      181 tatctttgaa gatatatttt caagtctaac ttcagttttc ttcgggttca cgtcgggctt
      241 tctattgtcg gtgcctaatg cattgtttat gtcctggtat tctccatatc gtaacattgt
      301 atatccttgg atacaatata tcagaaatat cccaccactt gcgtatgttc ctttagtagt
      361 aatttcggct ggggtcgggc tgttacctca agttattgta atagcgatag ctacttttct
      421 gactatgact gtaacaattt atcaaggcat tattaatatt gatccaaatt taattaaagc
      481 agcaagaatt ttaggtgcat cagattttga aatatttaaa agaattttgg ttccagcatc
      541 cattccattt attatgacag ctgtcagatt aggtacatcg atttctttaa ctacaatcat
      601 tgcagctgaa tctactggtg ctacatcagg tatgggaatg agaatacggt ctttaaataa
      661 ctcttttgat tcctcaggaa tgcttttgta tatcatattg attggagtaa ttggtttact
      721 gttaaataag ttagttgatt tgatagaaag gaagttaact ggatggcaac agaagtaaaa
      781 aaaattaagg tcaaaattga agaagtttca aaatcatttc agtcaattca tggtgaggtt
      841 gttgcactaa atcaaataaa tttagaaatc tttgagaatg aaattgttac agttgttggt
      901 ccatcaggtt gtggtaaatc tactctacta aacttggttg caggtttgga acctgtaact
      961 tccgggaaaa tttattgtga tgataatgaa attgttggcg ttggagttga gcgtggtgtt
     1021 gtttttcaac aatatgcact atttccttgg atgagtgtac aacagaatgt aatgttcgga
     1081 cctgaaatgc aaggaaaatc aaagttagaa gctagcgaaa tagctaacaa gtatattaaa
     1141 atggttggat tacaagaatt ttccaatcat tatccgaaag aattatcggg aggaatgaag
     1201 caaagagttg caatagcaag agcgtatgct gttaatccga cgattctatt aatggatgaa
     1261 ccatttggag ccttagatgc ccaaacgagg attcaactgc aagatgaatt gattgaaatg
     1321 ctggaacatg aacgaaaaac atgtttgttt atcacac
//
LOCUS       DBNFEG010000249         1315 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.251, whole genome shotgun sequence.
ACCESSION   DBNFEG010000249 DBNFEG010000000
VERSION     DBNFEG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.251"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            244..588
                     /locus_tag="AC1PNG_002068"
     CDS             244..588
                     /locus_tag="AC1PNG_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SSU0592/SSU0593 family protein"
                     /protein_id="HIP6702526.1"
                     /translation="MKRIVTKRSYKVGLCAMALALLMSTAGGVTAGAQDYHVESSGEL
                     KPMSEGVFMKLESSSNFIENEGIVVKSISNQNTNPNNLNESWYWKFWKKVKLFFKRFR
                     EGFGIQRLFGFK"
     gene            698..1042
                     /locus_tag="AC1PNG_002069"
     CDS             698..1042
                     /locus_tag="AC1PNG_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702527.1"
                     /translation="MKQANSKLAALTLITTLAVTSVIGTTVHAEEKVTADQTVAGKHK
                     PITDEQLQKAIGDRNPNNIKIQAKFENTNFKNQGITFIGKAEIPSKSSENSSWFWNLW
                     TRFTTFVKGIFS"
BASE COUNT          446 a          176 c          238 g          455 t
ORIGIN      
        1 aatagagggc tttaaatttt ttagggatat tctttttatt acttcgctta ttcgagacaa
       61 ggttctcttg aacggagcgt atcctatttc ctgattttta tattaatatt tttatttttt
      121 ggaatttaat ggttttttat ctaatctttc tctaaagttg gaatttacgt tatttttttg
      181 tgctatactg gcactacatt gtctagaaaa gggcgaatat tgtataaagg agacatttca
      241 aaaatgaaac gtattgtaac aaaacgtagt tataaagttg gtctgtgtgc tatggctctt
      301 gctctgttga tgagcactgc aggtggcgta actgcaggtg cacaagacta tcatgtcgaa
      361 tcatcaggcg aattaaaacc aatgagcgaa ggagtgttta tgaaattgga atcttcatct
      421 aatttcattg aaaatgaagg aatcgtggtg aaatctatta gtaatcaaaa tactaatcca
      481 aataatttga acgagtcttg gtattggaaa ttttggaaga aagtcaaact gtttttcaaa
      541 agatttcgtg aaggttttgg tatacaaaga ttatttggat ttaaatagtc aataattaaa
      601 gtgttattca aatgttcata atcttgtaat atttagcgca attaatttat aaaccttcat
      661 attaaaaaaa ctaatacttt gtaaaaggag acatttcatg aaacaagcta attcaaaact
      721 agcagcttta acactcatta caactcttgc agttacctct gttattggga caacggtgca
      781 tgctgaggag aaagtaactg ctgatcagac tgtagcagga aaacacaaac cgattacgga
      841 tgagcagctt caaaaagcga ttggagatag aaatccaaat aatattaaga tacaagctaa
      901 atttgaaaat accaatttca aaaatcaagg aataactttt attggaaaag ctgaaattcc
      961 atctaaatct tctgaaaact cttcatggtt ttggaatctt tggactcgtt ttacaacttt
     1021 tgtcaaaggt atttttagct aagatttcta tttttcgttg acttcatatg gatagagaac
     1081 agtggttcat gtgagccttt agtcaaatga aaagcgtata aaatattgga gagatctagg
     1141 catctaaact agagattgtg caagaaatta tgtaatagac ctcacatttt ttgcgaatga
     1201 gatgttgaca cgaattccat atttcttgag tctcggttgt aatttttctt agataaatta
     1261 aagagtgaaa agtttaggat gtaattgttc taaacttttt atattgcatc gataa
//
LOCUS       DBNFEG010000250         1264 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.252, whole genome shotgun sequence.
ACCESSION   DBNFEG010000250 DBNFEG010000000
VERSION     DBNFEG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.252"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>1264
                     /locus_tag="AC1PNG_002070"
     CDS             <1..>1264
                     /locus_tag="AC1PNG_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1250 family conjugative relaxase"
                     /protein_id="HIP6702528.1"
                     /translation="RQSSHKFELKQRLYFLMQQSKSFDDFLEKAVHLHVHIDFSQKHS
                     RFMMTDRAMTKPIRGRQLSKRDLYDEDFFRTHFAKQEIESRLEFLLNCVNSLEELLTK
                     AKELNLTIDLKQKNVVFTLEENGKQISLSHKKISDKKLYDVNFFQDYFKNKEVGDSEG
                     LENLQEQYHAFQEERDKDKVSTEEIEEAFETFKEKRDAVHEFEVEIAGHQIEKLVDEG
                     VYIKVSFGVKQSGLIFIPNYQLDILEEENQTKYKVYIRETTSYFVYNQEHSDKNQYIK
                     GRTLIRQLTNDSRVIPYRRPTVERLQEKITEINLLIELTETDKKYQDVKDELVAEIAV
                     LDIKLNQTNEKIATLNKMAEVLINLKSEDPNSRKLARYDFSKLNLTESITVEQVTEEI
                     RVLQEELGHYLDEYEGLARKLEIFVKILN"
BASE COUNT          485 a          186 c          254 g          339 t
ORIGIN      
        1 aggcagtcta gtcataaatt tgaactaaag caacgtcttt attttttgat gcagcagtca
       61 aagtcctttg atgatttttt agaaaaagcg gtgcacttac atgttcatat tgattttagt
      121 cagaagcata gtcgattcat gatgacggat agagcaatga caaaaccaat tcgaggacgc
      181 caactcagca aacgagattt atatgatgaa gactttttcc gtacacattt tgctaagcaa
      241 gagattgaaa gtcgtttaga atttttgttg aactgtgtca attctttaga agagttacta
      301 acaaaagcaa aagaattgaa tctaaccatt gacttgaaac aaaaaaatgt tgtatttact
      361 cttgaagaaa atggcaagca gattagtctg agtcataaaa aaataagtga taagaaatta
      421 tatgatgtca atttttttca agattatttt aaaaataagg aagtcggtga ttcagaagga
      481 ttagagaatt tacaggaaca gtaccatgct tttcaagaag aacgagataa ggacaaggta
      541 tccactgaag agattgagga agcctttgag acatttaagg aaaaacgaga tgccgttcac
      601 gaatttgaag tggaaattgc aggacaccaa atagagaagt tagttgatga gggcgtttat
      661 atcaaggtgt cttttggtgt aaagcagagc ggtcttattt tcattcccaa ctatcaatta
      721 gatatacttg aagaagagaa tcagacaaaa tataaagtct atatccgtga gacaacctca
      781 tactttgtct ataaccaaga acattcggat aagaatcagt atatcaaagg acgaaccttg
      841 attagacagc taaccaacga tagtcgagta ataccataca gacgaccgac tgttgaaaga
      901 cttcaagaaa agattactga gattaacctc ttgattgagc taactgagac agacaaaaaa
      961 taccaggacg ttaaagacga actggtcgca gaaatagcag tgctagatat caaactaaat
     1021 caaaccaatg aaaaaatcgc caccttgaat aagatggcgg aagtgcttat caacttaaag
     1081 agtgaagatc cgaatagtcg aaaattagca aggtatgatt tttcaaaact aaatttgaca
     1141 gaatcaatta cagtagaaca agtaaccgag gaaataagag tgttgcaaga agaactagga
     1201 cattatcttg atgagtacga aggattagct aggaagttgg aaatatttgt caagattctc
     1261 aata
//
LOCUS       DBNFEG010000251         1205 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.253, whole genome shotgun sequence.
ACCESSION   DBNFEG010000251 DBNFEG010000000
VERSION     DBNFEG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.253"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..336)
                     /locus_tag="AC1PNG_002071"
     CDS             complement(<1..336)
                     /locus_tag="AC1PNG_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIP6702529.1"
                     /translation="MNILFTLNDAFVPQVAACMGSIMRTLNEDDTCHFYLFSDGISQQ
                     NKEKLHQFVTDGGNKLTVVELENLESYFDFEVDTNGWASVVLARLLVDKLLPKEVDRI
                     IYLDGDTLVL"
     gene            complement(359..>1205)
                     /locus_tag="AC1PNG_002072"
     CDS             complement(359..>1205)
                     /locus_tag="AC1PNG_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HIP6702530.1"
                     /translation="VSLLPMMIQQAGSLSYDVVNYLAVVAFFVFYVNLLVDKVLTTRK
                     FINLILLALLLYLTKANNILLLALLFFVDFEFEGILSKFNLILKWIQKRRLPILILGL
                     GLVLLVSYLFLHNRGGVVHFVKVMINSLFINNRNDHLNGILTVGIFGYFGWFVTQLPL
                     WLIFIDIFIFTLLLFNDGNLKISKTEGLVSLFVLPVQVAIIVAGMYFAWTPTAIGPNA
                     MISQGAQGRYFTPFLVYLFPACLMLKEQVSVSVKQKYLIRLLTGTVVINFIMYLFLIV
                     DYYWL"
BASE COUNT          450 a          231 c          165 g          359 t
ORIGIN      
        1 taaaactaaa gtatctccat ctaaataaat gattctatca acttctttgg gtagtaattt
       61 atctacaagt aatcttgcaa gtacaaccga agcccaacca tttgtgtcaa cttcaaaatc
      121 aaaatatgat tccaagtttt ccaattcaac gactgtcaat ttatttcctc cgtcggtaac
      181 aaattgatga agtttttctt tattctgttg acttatacca tctgagaaaa gataaaaatg
      241 gcatgtatcg tcttcgttta atgttcgcat gattgagccc atacaagctg caacttgcgg
      301 gacaaaagca tcattcaatg taaataatat attcacgttt ccctcttcca ctatcttctt
      361 acaaccaata ataatccact atcaaaaata gatacatgat aaagttaatg actactgtgc
      421 cagtgagcaa ccgtatcaaa tacttctgtt ttaccgatac acttacctgc tcttttaaca
      481 ttaaacatgc cggaaataga tagactaaaa acggagtaaa gtatcttcct tgtgcccctt
      541 gagaaatcat tgcatttggt ccaatagctg tcggtgtcca tgcaaaatac atgcccgcta
      601 caatgatagc tacctgaaca gggagtacaa ataacgacac aagaccttct gttttactta
      661 tctttaaatt tccatcatta aataataata gcgtaaaaat gaaaatatct atgaaaatta
      721 accagagagg tagttgagtg acaaaccagc caaaataacc aaaaatacca actgttaaaa
      781 taccattaag atgatcgttt ctgttattga taaataaact gtttatcatc actttcacaa
      841 agtgcacgac accacctcta ttatgtagga atagatagct aacaagtaaa actagtccta
      901 atcccaaaat aagaattggt aatcttctct tttgaatcca ttttaaaatc aaattaaact
      961 tagataagat gccttcaaat tcaaaatcaa caaaaaacaa tagtgctaaa agtaaaatat
     1021 tatttgcttt tgttaaatat agtagcaagg ctagtaatat taagttaata aatttcctgg
     1081 tggtcagtac cttgtctacc aataagttaa cataaaaaac aaaaaatgca acaacagcca
     1141 gatagttcac cacatcataa gacagtgagc cagcctgctg aatcatcata ggtaaaagtg
     1201 agaca
//
LOCUS       DBNFEG010000252         1162 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.254, whole genome shotgun sequence.
ACCESSION   DBNFEG010000252 DBNFEG010000000
VERSION     DBNFEG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.254"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            154..1137
                     /gene="guaC"
                     /locus_tag="AC1PNG_002073"
     CDS             154..1137
                     /gene="guaC"
                     /locus_tag="AC1PNG_002073"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HIP6702531.1"
                     /translation="MFNETPVFDYEDIQLIPNKCIINSRSEADTTVTLGKYSFKLPVV
                     PANMQTIIDEDVAEMLAKDGYFYIMHRFDEAGRIPFIKRMHEQGLIASISVGVKEYEY
                     EFVTSLKADAPEFITIDIAHGHAESVIKMIQHIKKELPETFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHIESPGKTIEVDGEKFKEYYGSASEYQKGAYKNVEGKKIL
                     LPAKGHLKDTLVEMEQDLQSSISYAGGRDITSLKHVDYVIVKNSIWNGDSI"
BASE COUNT          366 a          210 c          258 g          328 t
ORIGIN      
        1 gccttaaagg tgatatatcc gaatattgtc tagataggtt tcttttttat tgacaaaaca
       61 caaacaataa gctatactat atcttgtaat aaggaaaaaa tacgaaaaac aaaccgtttt
      121 tctgttccaa cgttaagaaa aagaggtaga agcatgttca acgaaactcc agtttttgac
      181 tacgaagata ttcagctcat tccaaataaa tgtatcatca atagccgttc agaggcagat
      241 acaacagtga ctcttgggaa atatagcttt aaattaccag ttgttccagc taatatgcag
      301 actattattg atgaagatgt ggcagaaatg cttgccaaag atggttattt ctacatcatg
      361 catcgtttcg atgaagctgg tcgtattcca tttatcaaac gcatgcacga gcaaggtttg
      421 attgcttcta tctctgttgg cgtaaaagaa tatgaatatg agtttgtgac tagcttgaaa
      481 gctgatgccc ctgagtttat caccattgat attgctcatg gccatgcaga aagtgttatc
      541 aagatgatcc aacatatcaa gaaagaactg cctgagacgt tcgtcattgc aggtaatgtc
      601 ggtactccag aagcagttcg tgaactagaa aatgctggtg cagatgcgac aaaagttggt
      661 attggtccag gtaaagtatg tattacaaag gttaagactg gttttggtac aggtggttgg
      721 cagttggcag cccttcgttg gtgtgcaaaa gctgcccgta aaccaattat tgcggacgga
      781 ggtatccgta cccacggaga tattgccaaa tccatccgct tcggtgcaag catggtcatg
      841 attggttcgc tctttgccgg tcacattgaa agcccaggta aaacaattga agttgacggt
      901 gagaaattca aagagtacta cggttctgca tctgaatacc aaaaaggtgc ctataaaaac
      961 gtagaaggta agaaaatctt actccctgcc aaaggacatt tgaaagatac tctggttgaa
     1021 atggagcaag atttgcagtc atctatttct tatgcaggag gacgtgacat taccagcttg
     1081 aagcatgtgg actatgttat cgtgaaaaac tctatctgga atggtgactc tatctagtca
     1141 aagaggactt gcaatgcagg tc
//
LOCUS       DBNFEG010000253         1161 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.255, whole genome shotgun sequence.
ACCESSION   DBNFEG010000253 DBNFEG010000000
VERSION     DBNFEG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.255"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            79..558
                     /gene="rlmH"
                     /locus_tag="AC1PNG_002074"
     CDS             79..558
                     /gene="rlmH"
                     /locus_tag="AC1PNG_002074"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIP6702532.1"
                     /translation="MKIKLITVGKLKEKYLKEGIAEYSKRLGRFTKLDMIELPDEKTP
                     DKASQAENEQILKKEADRIMSKIGERDFVIALAIEGKQFPSEEFSQKLSDIAVNGYSD
                     ITFIIGGSLGLDSCIKKRANLLMSFGQLTLPHQLMKLVLIEQIYRAFMIQQGSPYHK"
     gene            583..>1161
                     /locus_tag="AC1PNG_002075"
     CDS             583..>1161
                     /locus_tag="AC1PNG_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HIP6702533.1"
                     /translation="MNQKEYRVFEGLRIACSLTFISGYLNAFTFVTQGGRFAGVQSGN
                     VISLAYFLAKGDLAQVVNFSIPILFFVFGQFFTYLARRYFEKQTWSWHFGSSVMMLVL
                     ILLTIILSPIMPASFTIASLAFVASIQVETFRRLRGAPYANVMMTGNVKNAAYLWFKG
                     VIEKDSELRKTGRNILLTIIGFMLGVIISTHLS"
BASE COUNT          370 a          169 c          229 g          393 t
ORIGIN      
        1 tgtgaaaaac tgtagccata tcctaatttt ctaaaaatat tttctgtcgt atgcttgtgg
       61 atatgttaca attattttat gaaaataaaa ttgattaccg ttggaaaatt gaaagaaaag
      121 tacctcaaag aaggtattgc agaatatagt aaacgattgg gacgatttac taagttggat
      181 atgattgagc ttcctgatga aaaaacacca gataaagcca gtcaggcaga gaatgaacaa
      241 atattaaaaa aagaagccga tagaattatg tctaaaattg gagagcgaga ttttgtcatt
      301 gccttagcga tagaagggaa acaatttcca tcggaagaat ttagtcaaaa gctatctgat
      361 attgcagtaa atgggtattc agatataact tttatcatcg gtggtagttt gggtctcgat
      421 tcttgtatta aaaaaagagc taatttgttg atgagttttg gacagttgac acttccacat
      481 caattaatga aattagttct catcgagcag atttatcgtg catttatgat tcagcaggga
      541 agcccatatc ataagtagtt tcacgtgaaa caaggagtga aaatgaatca aaaagagtat
      601 cgtgtttttg agggattgag aattgcttgt tcattaacgt ttatcagtgg ttatttaaat
      661 gcctttactt ttgtgactca gggtggtcgc tttgctggcg tacaatctgg aaatgttatt
      721 tccctagctt attttttagc taaaggtgac cttgcgcagg tagttaattt ttccattccc
      781 attttatttt ttgtattcgg acaatttttt acctacttag caagaaggta ttttgaaaaa
      841 caaacatggt cttggcactt tggtagtagt gtaatgatgt tagttcttat tttactaact
      901 atcattctct cacctataat gcctgcgtct tttacaattg ctagtctagc cttcgtagcc
      961 tctattcaag tagaaacatt tagaaggtta cgaggtgctc cgtatgccaa tgtgatgatg
     1021 acagggaatg tcaaaaatgc tgcttatctc tggtttaaag gagttattga aaaagattca
     1081 gaacttagaa aaacaggtag aaatatctta ttaactatta tagggtttat gctaggtgtc
     1141 atcatatcta ctcacctatc c
//
LOCUS       DBNFEG010000254         1143 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.256, whole genome shotgun sequence.
ACCESSION   DBNFEG010000254 DBNFEG010000000
VERSION     DBNFEG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.256"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(28..870)
                     /locus_tag="AC1PNG_002076"
     CDS             complement(28..870)
                     /locus_tag="AC1PNG_002076"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIP6702534.1"
                     /translation="MTRYTFSNGVTVPKIGFGTWQIPDGEVAYGAVSYALEAGYRHID
                     TAQIYGNEASVGRAIADSGLAREDIFLTTKVWNDKIGYEDTLASVEESMQKLQVDYLD
                     LLLIHWPNPKASRETIGWKKRNAQVWKALEELYRAGKVKVIGVSNFMEHHLEALLETA
                     EIIPMANQIMLAPGTPQSELVAYCKAKGILLEAYSPFGTGSLFQSQEAADLAKEAGCS
                     VAQLALAWSLDKGFLPLPKSTSPENIKANLEIDGLAISPTAVAKLDKLEGVKGRLDPD
                     QAEF"
     gene            complement(986..>1143)
                     /locus_tag="AC1PNG_002077"
     CDS             complement(986..>1143)
                     /locus_tag="AC1PNG_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018167057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HIP6702535.1"
                     /translation="VTMFTIALELKVLYDLPFFLTWLTTAAGELLSLLVGAVVIDKLS
                     KKINFEK"
BASE COUNT          352 a          268 c          183 g          340 t
ORIGIN      
        1 acatatccaa ccttatattc atatttttta aaattcagcc tgatccgggt caagtcgacc
       61 ttttactcct tctagtttat ccaactttgc aactgctgtc ggactaatag ccaagccatc
      121 aatttccaaa ttcgccttaa tattttctgg actagttgat ttcgggagag ggagaaagcc
      181 tttatctaaa gaccaagcta gtgccagctg agcaacacta caaccagctt ctttagccaa
      241 atcggctgcc tcttggcttt ggaaaaggct tcctgtacca aatgggctat aggcctccaa
      301 gagaatccct ttggctttgc agtaagcaac taattctgac tgaggagttc ctggagctaa
      361 cataatctga ttagccatcg ggataatctc agccgtttcc agcaaagctt ctaggtgatg
      421 ttccataaag ttagaaactc caattacttt cacttttcca gcacgataga gctcttctaa
      481 tgccttccaa acctgggcat tgcgtttttt ccatccaatt gtctcacgac tagcctttgg
      541 atttggccaa tggatcagta acaaatccag ataatccacc tgaagttttt gcatggattc
      601 ttctactgaa gcaagggtat cttcatagcc aatcttatca ttccaaacct tagttgttaa
      661 aaaaatgtcc tcacgagcta ggccactatc agctatagcc cgaccaacac tagcttcatt
      721 gccataaatt tgagcagtat caatatgacg ataaccagct tccaatgcat aagaaaccgc
      781 accgtacgcc acttcaccat cgggtatctg ccaagttcca aaaccaattt taggaacagt
      841 tactccattt gaaaacgtat atcttgtcat aaaagacctc ctatttcttc tatccccagt
      901 ataaactaaa ttgtcaatca atacaaaata atgcgaccta aaaataaaag acacccgaaa
      961 gtgtctgctc acatttattg tatacttact tttcaaaatt tattttcttg gatagtttat
     1021 cgataacaac agctccaact aatagagaaa gcaattctcc tgctgctgtt gtcaaccaag
     1081 ttagaaagaa tggtaaatca tataagactt ttaattctaa agcaatggta aacatagtta
     1141 ccg
//
LOCUS       DBNFEG010000255         1107 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.257, whole genome shotgun sequence.
ACCESSION   DBNFEG010000255 DBNFEG010000000
VERSION     DBNFEG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.257"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..719)
                     /locus_tag="AC1PNG_002078"
     CDS             complement(<1..719)
                     /locus_tag="AC1PNG_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702536.1"
                     /translation="MGGIGMRESSIIKNSLFWEWRCLTTSSQFYIYLILSGMYYLLLA
                     LLNFFEVGFGEYLLKGPGLDGIETISLLSYLLFQAQNIFLLQQLFSGLSTTNFNLTIL
                     RIGSIKDWFLVKLVSLIFLSLLLGLFQTVPLFFIGNMDYLFFLHFFVVNSTNFILFSS
                     LLFLLFHFDFSDAVSFCITYSLLVFIIFSGEILGENTNYFVTILARVAEYEKNSNLSL
                     FIAIFFEIILSAAILSFIKIR"
BASE COUNT          476 a          176 c          165 g          290 t
ORIGIN      
        1 tatctgatct ttataaaaga aagtatcgcc gcacttagta taatctcaaa gaatatagcg
       61 atgaaaagtg acaaatttga atttttctca tactcagcta cccgtgccaa tatcgtaacg
      121 aaataatttg tattttctcc taaaatttct cctgaaaaaa tgataaaaac gagtaaagaa
      181 taagtaatgc aaaacgagac agcatccgaa aaatcaaaat ggaacaataa aaagagcaat
      241 gaactgaaca aaataaaatt agtgctatta actacgaaaa aatgcaagaa aaacaaatag
      301 tccatattac ctataaaaaa cagaggaact gtttgaaaca accccaataa aagtgataga
      361 aatatcaagg acacaagctt gacaagaaac caatctttta tactcccaat tcttagtatc
      421 gttaagttga agtttgttgt tgataaacca gaaaatagct gttgcaacaa aaaaatattc
      481 tgtgcttgaa acaataggta agaaagtaga ctaatggttt ctatgccatc caacccagga
      541 ccttttagaa gatactcacc gaagccgact tcaaaaaaat tcaatagcgc taacaataag
      601 taatacatcc ctgataaaat tagataaata taaaactgag agctagtagt caaacaacgc
      661 cattcccaga ataaagaatt ttttataatg gaactctccc tcattcctat tcctcccact
      721 ctgtatttct tattttatat aggtttaaca aaatgaaaaa aagcaagaga attagccaat
      781 acaataactc cttgtggtaa cttactgatt ttaaaatata gtgatggata ggaaagactt
      841 gactaatggt tgcattgatc ggataaaacc agaaaacaga ttgtattaac aaaaatgaga
      901 aaagattagc catgactgta cttccttttt gaaaaagaag aaaattaaac aaggctattt
      961 ccgagaggaa aataagataa cataaaaaac taaacaaggg caaaagtata ctttgaaaat
     1021 tagaaacgta agatatttca taaacttctg atagaagtgg ttgatgtata gaagaaattg
     1081 aaaaagaaaa cgaatttacc aacccca
//
LOCUS       DBNFEG010000256         1105 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.258, whole genome shotgun sequence.
ACCESSION   DBNFEG010000256 DBNFEG010000000
VERSION     DBNFEG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.258"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(41..730)
                     /locus_tag="AC1PNG_002079"
     CDS             complement(41..730)
                     /locus_tag="AC1PNG_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIP6702537.1"
                     /translation="MGKVIIGISGNEQEFPTKSGRTYVTVASELADGVRQAGGVPMVI
                     PMGTPDLAKDYINMIDKLILSGGQHVDPSLYGQERLIDSNDYLLERDAFELALIEEAL
                     RQGKPIFAVCRGMQLLNVALGGSLEQEVAHHWQEGIAGTSHRLQVKPKSRIGQLFTQG
                     SPINSFHQQRIKELAPGLVATGLDPRDGTIEAYESRGNQALFGIQWHSEFLYNDCKQH
                     RDLFQYLVDIF"
BASE COUNT          352 a          245 c          180 g          328 t
ORIGIN      
        1 gcaattataa gcattgataa taaaaagagg gatacccctc ttaaaatata tctactaaat
       61 actgaaacag gtctcgatgt tgcttacaat cattatacaa aaattctgaa tgccattgga
      121 taccaaacaa agcttggttc cctctacttt cataagcttc tatcgtacca tctctaggat
      181 ccaaacctgt cgccaccagt cctggagcca attctttgat tcgttgctga tggaaggaat
      241 taatagggct cccttgggta aacagttgac cgattcgact tttaggtttc acttgtaaac
      301 gatgggatgt ccccgcaata ccctcttgcc aatgatgagc cacttcctgc tctaaactac
      361 cacctagagc aacattgagc aactgcatac cacggcaaac ggcaaaaata ggttttcctt
      421 gccgaagggc ctcttcaatc aaagctaact caaatgcatc ccgttccaag aggtaatcat
      481 tactatcaat caagcgctct tgcccgtata gactggggtc tacatgctga ccgcctgata
      541 aaatcaactt atcaatcatg ttgatatagt ccttggctaa gtcaggagtt cccataggaa
      601 taaccattgg tacaccacct gcctgacgca caccatctgc caattcactc gcaacggtta
      661 cataagttct accagattta gttggaaatt cttgctcgtt tcctgaaatt ccaatgataa
      721 cttttcccat aaaaagcccc tttctttcat tttttcaatt gtagcatggc tacctagccc
      781 tgtctaatat agaagtttta tggcttgata aaaaataact atcagaacta cctataaaca
      841 attgctcata aacaagagtt tgcctttagc agtttcatag ataaaatcta taccacgctt
      901 aagaaaaaac tatactggag ccagcataaa aaagtcaagc ttgataaaca ggcttttttc
      961 aagcttttat atagaagaaa tatcaaaaaa gtagctctct ttcaatctgt tatagtcaaa
     1021 agttataact ccctctaatt ttttacaatt atacaagagt cgttttttct gttaggatag
     1081 gggcatagaa atattttgga ggaaa
//
LOCUS       DBNFEG010000257         1076 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.259, whole genome shotgun sequence.
ACCESSION   DBNFEG010000257 DBNFEG010000000
VERSION     DBNFEG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.259"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(35..>1076)
                     /locus_tag="AC1PNG_002080"
     CDS             complement(35..>1076)
                     /locus_tag="AC1PNG_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIP6702538.1"
                     /translation="PSTNVVVRGNRIEKAGGDSLTVRDTDGALIEYNISADARYQNTG
                     YNAGIWPFQASNTVIQYNESFRTHGVQDGQGLDLDHISNNSVMQYNYSHDNEGGFMLI
                     MNWYEQTSPTVRYNISQNDKDKIFELARGGAQGTAIYNNTIYSDSKLTGRAGVIDMPS
                     TSGGTGVKDIFLFNNIFYFVDGEKMLVEASDAGKYKDNIHFYNNAYVGVEVPNDPRAI
                     TEGIELNGIGTGPIDNKSMIANTGKYLTGQLDGYKIPFNSVLANSGVSKEDAITYFYE
                     KLGIQQQIDFIENGSLNMSPIEAFSLARQTNSIDAIARSYPKIDGVNYDIDFFGESIL
                     TDNLSIGAAQVK"
BASE COUNT          339 a          216 c          149 g          372 t
ORIGIN      
        1 atttgactta atgaaaaata actagctgtt tcactcattt aacttgagca gcacctatag
       61 ataaattgtc tgttaaaatc gattctccaa agaagtcaat atcatagtta actccatcaa
      121 tttttggata tgagcgagcg attgcatcta tactattcgt ttgacgagct aaagaaaagg
      181 cttctattgg ggacatattt aacgacccat tttcaataaa atcaatttgt tgctgaatcc
      241 ctaatttttc atagaaataa gtaattgcat cttcttttga tacgccacta tttgccaata
      301 cactgttgaa aggaatttta tacccgtcta attgaccagt taaatatttc ccggtatttg
      361 cgatcattga tttattatca attggtcccg taccaatacc atttaattct atcccttcag
      421 tgattgctct aggatcatta ggcacctcga caccaacata ggcattatta taaaaatgaa
      481 tattatcttt atatttacct gcatcacttg cttcaacaag cattttctca ccatctacaa
      541 agtaaaaaat attattaaat aaaaagatat cttttactcc tgttccacct gacgtacttg
      601 gcatatcaat tacacctgct cgtccagtta atttcgaatc agagtagatt gtattattgt
      661 aaattgcagt tccttgtgct cctcctctag ccaattcaaa aattttgtct ttatcattct
      721 ggctgatatt ataacgaact gtaggagatg tttgttcgta ccagttcatg attaacataa
      781 acccaccttc attatcatga ctgtagttat attgcatcac tgaattatta gaaatatgat
      841 ctaaatctaa accctgacca tcttgaacac catgcgttct aaatgattca ttgtattgaa
      901 taactgtatt cgaagcttgg aaaggccata tacctgcatt gtacccagtg ttttggtatc
      961 tagcatcagc agaaatattg tattcaatca atgcaccatc agtatctcta acagttagag
     1021 aatcaccacc tgccttctca atacgatttc ctctaacaac tacatttgta gatgga
//
LOCUS       DBNFEG010000258         1070 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.260, whole genome shotgun sequence.
ACCESSION   DBNFEG010000258 DBNFEG010000000
VERSION     DBNFEG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.260"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(96..806)
                     /locus_tag="AC1PNG_002081"
     CDS             complement(96..806)
                     /locus_tag="AC1PNG_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5986 family protein"
                     /protein_id="HIP6702539.1"
                     /translation="MKYSYDIEREVIIKCLEAMSFTTRHLEQDYKVAISETSQNGIYQ
                     ATWARRSNMLKDLFDNSVVWKVLHIKRGIWQFDPILNIETGELIAFFSKNNFRTIRNR
                     YFKKGTSTHYSLSLLLKNERLYPEQEVEQLSLFDADSSEVKRREADLVRMLGEYCEHV
                     TKIRFLIVDYVGQEAVSASLEDYTPQFLSVSSVDISHLLPSSAGIIRTEAIRDEQVND
                     LVDEPQLVTLKPIVEGIL"
BASE COUNT          360 a          225 c          138 g          347 t
ORIGIN      
        1 tttagaaact ccaacgtttt ccgccaattg gctaatggaa taatgtctaa aatatctcga
       61 ttctcgaagt ctatctccat taaattcttg agccattata atattccttc tactattggt
      121 tttaatgtaa cgagttgagg ctcatctact aaatcattca cctgttcatc acgtatggcc
      181 tcagttctaa ttatcccagc tgaacttgga agtaagtgcg aaatatccac tgaactaact
      241 gataaaaatt gtggagtgta gtcttctaac gaagcactca cagcttcttg acctacataa
      301 tcaacaatca aaaatctaat cttagtcaca tgttcacaat actcaccaag catcctaacc
      361 aaatcagctt ctcttctttt cacttcagag ctatccgcat caaaaagaga aagctgttct
      421 acttcttgtt cagggtaaag tctctcattt ttcaagagta aacttaagga ataatgagta
      481 gaggttcctt ttttaaaata tcgattacga attgtcctaa aattattttt ggagaagaag
      541 gctattagtt cacctgtctc aatattcagt atagggtcaa attgccaaat ccccctctta
      601 atatgcaaaa ctttccaaac aacactatta tcaaataagt ctttaagcat attcgatctt
      661 cgtgcccaag tagcctgata aattccattt tgactagtct ctgagatagc aactttataa
      721 tcctgctcca gatgcctagt tgtaaaactc atcgcttcca aacacttgat gattacttct
      781 ctttcaatat cataactata tttcatttat tcacctcaat tcgtttgtta tttaacttca
      841 ttataacaga ccaaggagac aaaaacaaga aaaactacca aaaaagtcaa ccaaagtgtg
      901 tagaattaaa taatcccatc tcctcagcga tgctaatatc tttctcctta cacatactca
      961 catctaagta gattaaatag ctagttacta aatgctataa taaaaaaatt aggacagcaa
     1021 gctatttgct atcctaataa atattaccaa tagttgttca atccgagatt
//
LOCUS       DBNFEG010000259         1029 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.261, whole genome shotgun sequence.
ACCESSION   DBNFEG010000259 DBNFEG010000000
VERSION     DBNFEG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.261"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            176..646
                     /locus_tag="AC1PNG_002082"
     CDS             176..646
                     /locus_tag="AC1PNG_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIP6702540.1"
                     /translation="MEFEKVYASVKGIVNKARKEFYIKLWDRDDWEQEGMMTLFELLE
                     AQPWLVDEQVQLYCYFKVKFRNRIKDRIRKQESQKRKFDRMPHEDIHELSHAIQSPGL
                     INDELLMLRGALRDYRKNLSNDQLDKYEKLISGQCFNGRREMIRDLQIHLKDFR"
BASE COUNT          402 a          148 c          198 g          281 t
ORIGIN      
        1 tagaactata ccaaatcact cccttctgaa gcagaatgca atgtttgcca tagagtatca
       61 atagcaaata tacttaaaaa aagaaaaatc aaataaagta tgctagacaa aaatgtcctg
      121 tagaaagaca gcacttttcg ataaacttaa accatcaaca aaaggagatt aagtaatgga
      181 attcgaaaaa gtgtacgcaa gcgtcaaagg tattgtaaat aaggctcgaa aagagtttta
      241 cattaaacta tgggatcgag atgattggga acaagaagga atgatgacct tgtttgaatt
      301 gttggaagct caaccgtggc tagttgatga acaagttcaa ttatattgtt attttaaagt
      361 caagttcaga aatcgaatca aggatcgtat ccgcaaacag gaaagtcaaa aacgcaagtt
      421 tgaccgtatg ccacatgaag atattcacga attatctcac gcaatacaat caccgggatt
      481 aataaacgat gaactattaa tgctaagagg tgccttgaga gattatcgaa aaaatctgag
      541 taatgatcaa cttgataaat acgaaaaatt aattagcgga caatgtttta atggtcgccg
      601 tgaaatgata cgtgatttac aaattcattt gaaagacttt cgctaaagct agtcactaac
      661 atcatgttaa tcgaattaag aaattgggca gaagcaccat aaaaagttct gttcagtttc
      721 tttttttata ggttatagca aagagaagaa ggaatagggg gaaaaaaaca aatttcaagt
      781 taaaattagc catcctatct tatgaagagt aggggtgtga ctaaatgtga tactcagcca
      841 atagtaacct aagtaattga aagactgatt cagcggtatt atttagaatt aaccttatgc
      901 tttagtatct acatagaact ttcagtgtaa aaaaatccca aaaatcggtt gacaatgtat
      961 ataagtcgtg atagaataaa tgagttgtct cttgagggac tggttaacag aggaacgaaa
     1021 aaaagtttc
//
LOCUS       DBNFEG010000260          993 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.262, whole genome shotgun sequence.
ACCESSION   DBNFEG010000260 DBNFEG010000000
VERSION     DBNFEG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.262"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(213..977)
                     /locus_tag="AC1PNG_002083"
     CDS             complement(213..977)
                     /locus_tag="AC1PNG_002083"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIP6702541.1"
                     /translation="MKRLLRRIRPTKPLKELTWIDLIIITTILCGNAIYTSTMQWIAS
                     LSATETVETGVLSFSPADNWWALANQGKLFLFALVYLLIRNYDFKQLKVKLEWRVLLW
                     GPLIFIGAGLISDLAFTAFSYIPGLSGGYNFLGYLPYYDWNIMTVLNRFLAVDYSTVI
                     YSLFNGFYEEFFFLGLLLSTDKKKRSLVLLFSTIVRISFHTYQGMVSALVIGVAFGLF
                     YYYMYTRKNDNLLPYFLGHALADMVGTSFFSLFIAG"
BASE COUNT          353 a          188 c          178 g          274 t
ORIGIN      
        1 cattttaaca taaataaacc attaattcaa aaaaatatgg gataaaaaat atgataaagc
       61 aatcgaaatt tgactgaaca gaataaatat atcagcacaa taaaacgggt gatgataccg
      121 agttgataga aactacgtat catcatccgt ttgtcagctg tagggagaaa gaaattttat
      181 ccgattagct tgatgtccgt tcttctcatt gtttaccctg caataaacaa tgagaagaaa
      241 cttgtaccaa ccatatccgc aagagcatgt cctaagaaat atggcaataa attgtcattt
      301 ttccttgtgt acatgtagta atagaaaagg ccgaaggcta caccaataac aagagcagat
      361 accattcctt gataggtatg gaaagatata cggacaattg ttgaaaagag taagaccagc
      421 gaacgctttt tcttatctgt actcaacaat aagcctaaga aaaagaactc ttcatagaag
      481 ccattaaaca aggaatagat aactgtgctg tagtcaacgg ctaggaaacg gttgagcact
      541 gtcattatat tccagtcata gtaaggcaag tatcctagga aattataccc accagacaga
      601 cctggaatat atgaaaaagc tgtaaatgcc aaatcgctaa tcagaccagc accgatgaaa
      661 atcagtggtc cccagagcaa aactctccac tctaatttta cttttagctg cttgaaatca
      721 taattccgaa tcaataaata caccagggca aacaggaata attttccttg attggctaga
      781 gcccaccaat tatcggctgg actaaacgac agaactcctg tttcaacggt ttctgttgcg
      841 gaaagtgatg ctatccattg catggttgag gtataaatag cattaccaca taaaatcgtt
      901 gtgataataa tcaaatctat ccaagtgagt tcttttaggg gctttgttgg acgaatgcgt
      961 ctcagtaatc ttttcacttt ttcctcctta tat
//
LOCUS       DBNFEG010000261          954 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.263, whole genome shotgun sequence.
ACCESSION   DBNFEG010000261 DBNFEG010000000
VERSION     DBNFEG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.263"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            138..>954
                     /locus_tag="AC1PNG_002084"
     CDS             138..>954
                     /locus_tag="AC1PNG_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HIP6702542.1"
                     /translation="MEKHRVRQHIVPQAYLKKFAQKNVNNSGANISVYQKANNRQFNC
                     AVKNVAFEKNYYDVTNKENTKYWEEHFATHIEPLYGNELTNIISSIMLSNSNENVLNP
                     SQKFSLAMMIVFQIIRVPKFLKGRFQNGEVIFDETIRELIQLMPMEFISKNIEVLEDL
                     KRERKNLIKDITIELIAQHDMLDRLAKYIENRTWLFYVNNSEVPFVTSDNPALMYNFT
                     TNSINREDVGIGRRDTIIYYPLTSRVLLELLPQNDNCKHFDNFIMEIGNEKLKF"
BASE COUNT          371 a          119 c          175 g          289 t
ORIGIN      
        1 ataataggct gaaatattca ttcgctgaac agaatctttt gatttatcca ctggtaaaag
       61 acggaattta gttggaatgg gctaatcaag taaaaaacag aatttcatta taaaactttt
      121 taaggaagat taagtggatg gaaaagcata gagttagaca acacatagtt cctcaagcat
      181 atttaaaaaa atttgcacaa aagaatgtca acaatagcgg agcaaatata tctgtctatc
      241 aaaaagcaaa taatagacag tttaattgtg cggtcaaaaa tgttgctttc gaaaaaaatt
      301 actatgacgt aaccaataaa gaaaatacaa aatattggga agagcatttt gcaacacaca
      361 ttgagccctt atacggcaat gagctaacaa atataatttc tagcattatg ctttctaatt
      421 cgaacgagaa cgtgttaaat ccaagtcaga agttttcttt agcaatgatg attgtatttc
      481 aaataatcag agtaccaaaa tttttgaaag gaagatttca aaacggagaa gtgatttttg
      541 atgaaacaat tagggagttg atacaactaa tgcctatgga gtttatttct aaaaatatag
      601 aggtattaga ggatttaaaa agagagcgta agaatttaat aaaagatatt acgatagagt
      661 tgattgctca gcatgatatg ttggatagat tagcaaaata tattgagaat agaacatggt
      721 tattttacgt caataattcc gaagttccat ttgtgactag cgataatcct gcgctaatgt
      781 ataactttac taccaattca attaaccgag aagatgttgg gattggaagg agggatacaa
      841 ttatttatta tcctttgaca agtagggttt tgcttgagct attgcctcag aatgacaatt
      901 gtaaacattt cgacaatttc ataatggaaa ttggaaatga aaaattaaag ttta
//
LOCUS       DBNFEG010000262          948 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.264, whole genome shotgun sequence.
ACCESSION   DBNFEG010000262 DBNFEG010000000
VERSION     DBNFEG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.264"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            184..342
                     /locus_tag="AC1PNG_002085"
                     /pseudo
     CDS             184..342
                     /locus_tag="AC1PNG_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167702.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative cross-wall-targeting lipoprotein signal
                     domain-containing proteiin"
     gene            533..>948
                     /locus_tag="AC1PNG_002086"
     CDS             533..>948
                     /locus_tag="AC1PNG_002086"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIP6702543.1"
                     /translation="MSLINPIPDTNWLKNNKDNLRISDEAYLELVQIYDLIDSIDWEY
                     SRINDDATLIDKINQFATNRKKTTVSVVYIVKNEENFILKSLASIIDFADEIIIVDTG
                     STDGTLECITELSREKIFLYTFEWCDDFSKARNYANS"
BASE COUNT          330 a          132 c          173 g          313 t
ORIGIN      
        1 caagccttct tttttatgat ggtattgaac ttgggctacg agctgtacaa aaaagagagt
       61 ttctcctaga acggaaagtt cttcgtcaaa actcctattt ttgtcgtgct cgcttaacgc
      121 cctcgtatct aataaagagt gacagtttgt tgctacttat caaaagaaaa gaaggacttt
      181 tttatgacca agcaatgtca ttatcacttt ttagtcaatc aggaaaaagc agaaaagtat
      241 gtcttccgta aaagcaagaa gtaccgaacc ctgtgttcgg tagctcttgg aagtatggtg
      301 acggctgttg tcgcttgggg tggccaagta gcacaagctg actaagttgt aacactttcg
      361 ctagataata ccgttcagag attctacaat tgtgattatg aactagcata agtctgctat
      421 ttaaagttac acttaagaac aaaaatgact attttaagaa caaactacct tttgatatag
      481 tttttgttgt ataatatctt caatgaaaat aatttaaggc tggaagacac gtatgagttt
      541 aataaatcct attccagata ctaactggtt aaagaataat aaagataatt taaggatttc
      601 ggatgaagcc tatcttgaat tagtacaaat ttatgattta atagactcca tagattggga
      661 gtattcaaga ataaatgatg acgcaacttt gatagataaa ataaatcaat ttgcgactaa
      721 tagaaaaaaa acaactgtat ccgtagtata tattgtaaaa aatgaagaga actttatttt
      781 aaaaagttta gcaagtatta ttgattttgc tgatgaaatt attattgttg atacaggttc
      841 tactgatggt actttagaat gtataactga gttatcacgt gaaaaaattt ttttgtacac
      901 atttgaatgg tgtgatgatt tttcaaaggc tagaaattat gctaattc
//
LOCUS       DBNFEG010000263          935 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.265, whole genome shotgun sequence.
ACCESSION   DBNFEG010000263 DBNFEG010000000
VERSION     DBNFEG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.265"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..881)
                     /locus_tag="AC1PNG_002087"
     CDS             complement(<1..881)
                     /locus_tag="AC1PNG_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6702544.1"
                     /translation="MVKNNNIAVKVEHVSKSFKLPTESSQSLRTTLVNLFKGIKGYVE
                     YNVLQDISFEVEKGDFFGIVGRNGSGKSTLLKILSQIYVPERGTVAVDGKLVSFIELG
                     VGFNPELTGKENVYLNGAMLGFTAEEIDAMYDDIVEFAELSEFMNQKLKNYSSGMQVR
                     LAFSVAIKAQGDILILDEVLAVGDEAFQRKCNDYFLERKKSGKTTILVTHDMGAVKKY
                     CNKALLIEKGYVKALGEPDDVANQYSFDNVLASISEVTENEEPLHQSDIVKDLQINLV
                     SKNQIEPDESIEIEFRYT"
BASE COUNT          290 a          210 c          116 g          319 t
ORIGIN      
        1 gtatatctaa actcgatttc aattgattca tctggttcaa tttgattttt tgagaccagg
       61 ttaatctgca aatcttttac aatatcactc tgatgcagag gttcttcgtt ttctgtaact
      121 tcgcttattg aggcaagcac attatcaaaa ctgtattgat tagctacatc atcaggttct
      181 cctaatgcct tgacgtatcc tttctctata agaagagctt tattacaata ctttttcaca
      241 gctcccatat catgggtgac aagaatggtt gtttttccac tttttttacg ctctaggaag
      301 taatcattac atttacgctg aaaggcctca tcaccgactg ctagaacctc atcaagtatc
      361 agaatatctc cttgagcttt aatggcaact gaaaaggcta aacgtacctg cataccacta
      421 gaatagtttt tcaacttttg attcatgaat tcactcaatt ccgcaaattc aacaatatca
      481 tcatacattg catcaatttc ttctgcagta aatcctaaca tcgcaccgtt taggtataca
      541 ttttccttac cagttagttc cggattaaac ccaacaccaa gttcaataaa agaaactaat
      601 ttaccatcca cagctacagt ccctctttca ggaacatata tttgagataa aatttttaac
      661 agtgtagact taccagaacc attgcgccca acaattccaa aaaaatctcc tttttcaact
      721 tcgaaactga tatcctgcaa aacattatac tctacgtagc ctttaatccc tttaaataaa
      781 ttgactaaag tagttcttag actctgacta ctctccgttg gaagcttaaa actcttgcta
      841 acatgttcta ctttcacagc aatattatta ttttttacca ttacaaaatc tccgcaaact
      901 ttttggcatt ttttctaaac acagttatac ccaat
//
LOCUS       DBNFEG010000264          931 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.266, whole genome shotgun sequence.
ACCESSION   DBNFEG010000264 DBNFEG010000000
VERSION     DBNFEG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.266"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..379)
                     /locus_tag="AC1PNG_002088"
     CDS             complement(<1..379)
                     /locus_tag="AC1PNG_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HIP6702545.1"
                     /translation="MKIEKLFLRLAIPFLLISIFVMPVTKVPDEATHAFMSWNILFDS
                     PTSRDTEAMNELRAADFAYSEPEIHAVDSTEYSSLFTKIRDFSKDKLSIDFSLKSLMS
                     TPQLIGLVVGKLIYPSYGMMVTIG"
     gene            complement(376..>931)
                     /locus_tag="AC1PNG_002089"
     CDS             complement(376..>931)
                     /locus_tag="AC1PNG_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIP6702546.1"
                     /translation="FFERGVIGTGVQRDFNILFMPVFSMNILLILFRPMITQLAIYRR
                     AGDYNQFKQYQKRIVKMVVGLAVLVLVGGIVLGIPALNILYGTNLNKYWLSFIITMLG
                     GIASTFATICDNMLTVLRKQKYLVISFAISCLLSILISNPLVEYYGILGAAIAFVSSM
                     WTWFLISFVIYLKLQKHLNWEVQE"
BASE COUNT          345 a          188 c          124 g          274 t
ORIGIN      
        1 gccctatagt gaccatcata ccatatgacg gataaatcaa tttacctaca actagaccaa
       61 ttagctgagg tgtactcatt aacgatttca gagaaaaatc gatagataat ttgtcctttg
      121 aaaaatcacg aattttggta aaaaggcttg aatattctgt agaatcaact gcatgaatct
      181 ccggttctga atacgcaaaa tctgctgccc gtaattcatt cattgcttca gtatctctac
      241 ttgtcggaga atcaaataat atattccacg acataaaagc atgtgttgct tcatcaggaa
      301 cctttgtcac aggcataaca aatattgaaa tcaacaaaaa ggggattgct aaccttaaaa
      361 ataatttttc tatcttcatt cttgtacttc ccaattcaaa tgtttttgca attttagata
      421 tattacgaaa gaaattaaaa accaagtcca catcgaacta acaaaagcaa tagctgcccc
      481 caatattcca taatattcta ccagtggatt agaaatcaat atggataata aacaagaaat
      541 tgcaaatgaa attacgagat acttttgctt cctcaaaaca gtaagcatgt tatcacaaat
      601 ggttgcaaag gtactcgcaa ttcccccaag cattgttata ataaagctga gccagtattt
      661 attaagattc gttccgtaga gaatatttaa tgctggaatc cccaatacta ttcccccaac
      721 caaaactaac acagccaatc cgacaaccat tttaacgata cgcttctgat attgtttaaa
      781 ttgattatag tccccagctc ttctatatat agccaactga gttatcatgg gtctaaacaa
      841 aatcaacaaa atattcattg aaaaaacagg cataaataaa atattaaaat ctctttgaac
      901 acctgttcct ataacacctc tctcaaaaaa a
//
LOCUS       DBNFEG010000265          930 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.267, whole genome shotgun sequence.
ACCESSION   DBNFEG010000265 DBNFEG010000000
VERSION     DBNFEG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.267"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            379..888
                     /locus_tag="AC1PNG_002090"
     CDS             379..888
                     /locus_tag="AC1PNG_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702547.1"
                     /translation="MLKYIRALPLVIVAGAVVYFTIFICIDNGDWTYVVTRALGLKEE
                     HANNVSLIVIGTQSLFLTSDWFYKRIRAFLNRRLEKAKGLNRQIYLGYKAGIEFLMAL
                     PKRTILFTFYLLLVVAESVGLIEKAKDYAAVIIFIIAVDRVTKIWPEEKRRMIEFSQK
                     IKKRIKSVG"
BASE COUNT          272 a          146 c          213 g          299 t
ORIGIN      
        1 gattggaagt cttcttcttt ttggggtaag taaaaaatgc taagggtgta cagttgtcgt
       61 acctgaaatt ctagcccctg cttgaccgta cttggtataa catattacaa tatatacaag
      121 aacctgtgtt cacagttcat cgctttcatt ggagtgtttg aaggtggaga taaggaaagg
      181 agattcctga caaattaggt ggtaggcgaa aactgccaca ggagcttttt actcgtcgaa
      241 gcaataaagt gaaactaaat ggtgatagag tcaagtattc cttcaaaaaa ttgccttgat
      301 gagcaaaaac gatttcttgc ataagaacac tttgtttatg aatataggtt ctaagatttg
      361 accaagagag gttattatat gttgaaatat atccgtgccc ttccccttgt tatagtagcg
      421 ggagcggttg tttactttac tatttttatc tgtattgata atggtgattg gacctatgtt
      481 gtgactagag cgctagggct taaggaagaa catgccaata atgtttctct gattgtaatt
      541 ggtacccaat cactttttct aacttcggat tggttttaca agcggattcg tgcctttctc
      601 aatcgtcgtt tggaaaaggc caaagggctt aatcggcaga tttatctggg ctataaggca
      661 gggattgagt ttttgatggc gttacccaaa cgaactattt tattcacatt ctatctactt
      721 ttagtagttg ctgaaagtgt tggtctgatt gaaaaagcca aggactatgc tgcggtaatc
      781 atcttcataa ttgctgttga ccgcgtgacc aaaatctggc cggaggaaaa gcggagaatg
      841 attgagttta gccagaagat taagaaacgg attaagagtg tgggataagt caagattaaa
      901 tattttaaga agacagctat gtcttctttt
//
LOCUS       DBNFEG010000266          923 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.268, whole genome shotgun sequence.
ACCESSION   DBNFEG010000266 DBNFEG010000000
VERSION     DBNFEG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.268"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>923
                     /locus_tag="AC1PNG_002091"
     CDS             <1..>923
                     /locus_tag="AC1PNG_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HIP6702548.1"
                     /translation="NNETIIENKSNSSDFSEIFTKGEEIPNLLSRLQRDNTNKYPVSF
                     KMNREGYFLYESPSNRKKAELDRILNGSEEQLSRELAYSNSGTDFYQYLSSEYKYLKV
                     DIKEKLLSLVIQLSLKYTSSRIMDYIIQEYFNDKIPRYARNKPYSKEITTSIIDFWNT
                     LLDDKKLDFSEKLYFLEKHYIFSFKELGEIFDNLSIDKDSIKTLKRRDFYLLTYLSSK
                     NLWNNFSEWEDNVWYVIDEMSDHEFISFWILQGIIFNGMDYYAFDYKPADKKYTIWIK
                     KYSFDRINEIEDYEDTVIKKIQSRLDLLEIKG"
BASE COUNT          351 a          114 c          156 g          302 t
ORIGIN      
        1 aataatgaaa cgatcattga aaataaaagc aattcgtcag attttagtga gatatttact
       61 aaaggagagg aaattcccaa ccttctgtct cgcctacaac gtgataacac aaacaaatat
      121 ccagtaagtt ttaagatgaa tagagaggga tattttcttt atgaatcgcc ttcaaatagg
      181 aaaaaggcag aactagatag gatattaaat ggatcagaag aacaattgtc tagggaatta
      241 gcttactcaa atagtggtac agatttttat cagtatcttt catctgaata caagtatcta
      301 aaagttgata taaaagagaa gttactatca ttagttattc aactttcact taagtacacc
      361 tctagtcgaa taatggatta tattattcaa gaatatttta atgataagat tccaagatat
      421 gctcgaaata aaccatatag caaagagatt actaccagta ttattgattt ttggaatact
      481 ttattggatg ataagaaatt agatttttca gaaaaactat attttttgga aaaacattat
      541 attttttcat tcaaggagct aggagaaata tttgataatc tatccattga taaggactcg
      601 atcaaaacac taaaacgtag ggatttctat ttgctaactt acttatccag caaaaatctc
      661 tggaataatt tttccgaatg ggaagacaat gtttggtatg ttattgatga aatgagtgat
      721 cacgagttta tttctttctg gatattacaa gggattatat ttaatggcat ggattattat
      781 gcttttgact ataagccagc tgataagaaa tatactatct ggataaaaaa atatagtttt
      841 gataggataa atgaaattga agattatgaa gacactgtta taaaaaagat tcaatcacgg
      901 cttgatttgc tagaaatcaa ggg
//
LOCUS       DBNFEG010000267          917 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.269, whole genome shotgun sequence.
ACCESSION   DBNFEG010000267 DBNFEG010000000
VERSION     DBNFEG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.269"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>917
                     /locus_tag="AC1PNG_002092"
     CDS             <1..>917
                     /locus_tag="AC1PNG_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIP6702549.1"
                     /translation="NILKTLFRHQKSSRTRISKSLKITPATITNMISYLVAENKVIET
                     GDEVRDYIGSGRSRRLITVNSQYRHYIGIEINANGIYLAVTDTIGNLIKNSNIKITEY
                     DSKKINEIIIQLVTETLEKFSYLEFGALGIAVPGHFDSKSGHIISNNVKWIYFDLAVI
                     KESISIPIFLENNINCMAIGAYLFHPESSPEQFLFIHIGPGLFCSFFDSEHILQNKNF
                     YIGEIGHTVVDLNGPSCECGKRGCLQTYISDTWLINNARFLFENVQGSIIKTLVEKPE
                     DITLDVVYNAYRLGDGFIIEKIESGIDFL"
BASE COUNT          333 a          122 c          149 g          313 t
ORIGIN      
        1 aaatatatta aaaactttat ttagacatca aaaaagctct agaacaagaa tatctaaatc
       61 tttaaaaatt actccggcaa ctattacgaa tatgattagt tatcttgtag ctgaaaataa
      121 agttattgag actggcgatg aggttcgaga ttatattgga tcaggtcgta gtagacgact
      181 tattacagta aattctcaat atagacatta tattggaata gagattaatg ccaatggaat
      241 ctatttagca gtcacagaca ctattggaaa cctaatcaaa aacagcaata taaaaattac
      301 agaatatgat tccaaaaaaa taaatgaaat aataatccaa ttagtaaccg aaactctaga
      361 aaagttttcc tatcttgaat ttggagctct cggtattgct gtaccaggcc actttgattc
      421 aaaatcagga catattattt caaataatgt aaagtggatt tattttgatc ttgccgtaat
      481 taaagaaagt atttcaattc ctatttttct agagaacaat attaattgca tggcaattgg
      541 tgcatatctt tttcatcccg aaagttcgcc agagcagttt ttatttattc atattggacc
      601 tggattgttc tgttcatttt ttgatagcga acatatttta caaaacaaaa acttttatat
      661 tggagagatt ggtcatacag tagttgattt aaacggacct tcttgtgaat gtggaaaacg
      721 cggatgcctt caaacttata tttctgatac ttggttaatt aataatgcaa gatttttatt
      781 tgaaaatgtt caaggttcta tcataaaaac tttggttgaa aaaccagaag atattacatt
      841 agatgttgta tacaatgcat acagactagg agatggtttt attattgaaa aaattgaatc
      901 agggattgat tttttag
//
LOCUS       DBNFEG010000268          913 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.270, whole genome shotgun sequence.
ACCESSION   DBNFEG010000268 DBNFEG010000000
VERSION     DBNFEG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.270"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(181..885)
                     /locus_tag="AC1PNG_002093"
     CDS             complement(181..885)
                     /locus_tag="AC1PNG_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HIP6702550.1"
                     /translation="MKIHVVNNQVEGATVALDILREKLNGGAKVLGLATGSSPLEFYR
                     LIRESDLDFSNVTSVNLDEYVGLGEESDQSYIHFMKENLFNDKPFKKSYLPNGLATDV
                     VAETERYNKILAEHPVDFQILGIGRNGHIGFNEPGAPFDGQTHLVELAPSTIEANARF
                     FDNPEDVPKQAISMGIANIMAAKTIVLMAYGQEKADAIKATVEGAVTEDVPASVLQNH
                     DNVILILDQAAASKLV"
BASE COUNT          266 a          199 c          164 g          284 t
ORIGIN      
        1 ataacaactg atatggatta tcagaatagc aaaaggcatg cccttaaaaa tataagttac
       61 atgccttttc acaatttttg tcaaattcat aatggaaaaa gaacctagat tcattcagaa
      121 attatactta ttgtactgtt acaaccaact ttatgtctaa gaataatcgt ttacaacata
      181 ctacactaat ttactcgctg ctgcttggtc gaggatgagg atgacattgt catggttttg
      241 gaggacgctt gctgggacat cttctgtcac tgccccttcc acagtcgcct taatggcgtc
      301 agctttttcc tgaccatagg ccatgagaac aatggtcttg gctgccataa tgttggcgat
      361 gcccatggag atagcttgtt ttggtacatc ttctggattg tcaaagaagc gggcgttggc
      421 ttcaatggtt gatggcgcca attcaaccag atgagtctga ccatcaaatg gagcacctgg
      481 ttcgttgaaa ccgatatggc cgttgcgacc gatacccaaa atttggaaat caaccggatg
      541 ttcagccaaa attttattgt aacgttctgt ttcagcaact acatctgtcg ccaaaccatt
      601 cggtaaataa gatttcttaa acggtttgtc attgaaaaga ttttccttca taaaatgaat
      661 ataggattgg tcgctctcct cacctagacc aacatactca tccaagttta cagatgtcac
      721 attcgaaaag tctaaatctg actcacgaat caatctgtaa aattctaacg gactggagcc
      781 tgttgccaaa cctaatacct tggcacctcc gttcaatttt tctctcaaaa tgtctaatgc
      841 tacagttgct ccttcaactt gattgtttac tacatgaatt ttcataacac acctctttgg
      901 tctataccat tta
//
LOCUS       DBNFEG010000269          909 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.271, whole genome shotgun sequence.
ACCESSION   DBNFEG010000269 DBNFEG010000000
VERSION     DBNFEG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.271"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            38..838
                     /locus_tag="AC1PNG_002094"
     CDS             38..838
                     /locus_tag="AC1PNG_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6702551.1"
                     /translation="MSSIQAENIQVSYDNRIIIDELSTSIPKGNITTIIGANGCGKST
                     LLKALTRIIPVQEGAIYLDGQAISQLPTKEVAKKLALLPQVLEATEGISVYELVSYGR
                     FPHQNGLGHLTDQDREKINWALEVTQTAPYARLPVDDLSGGQRQRVWIAMALAQDTDT
                     IFLDEPTTYLDLNHQLEVLELLKELNQSRQKTIVMVLHDVNLSARFSDYMIAMKEGDI
                     RYHGSVSNMMTTEILRDIFSIEPQLMQVAGQDYPILLTYDLKNNKEKK"
BASE COUNT          277 a          173 c          189 g          270 t
ORIGIN      
        1 aattattaat gaatcatatt ctggaaagga ggagtttgtg tcttcgattc aagcggaaaa
       61 tatccaagtc tcttacgaca atcggattat tattgatgaa ttatcaacaa gtattccaaa
      121 aggaaatata actacgatca ttggtgccaa tggctgtgga aaatcgacct tgcttaaggc
      181 gttgacaagg attattcctg ttcaagaagg ggctatctat ctagatggac aagccatttc
      241 acaactgcca accaaagaag ttgcaaaaaa attggccctg ctccctcagg tcttagaagc
      301 gacagaaggg atttccgttt atgaactggt ctcctatggt cgctttccgc atcaaaatgg
      361 gctggggcat ctcactgatc aggatagaga gaaaatcaac tgggccttgg aagtcaccca
      421 gaccgctccc tacgctaggt taccagttga tgatttatca ggtggtcaga ggcaacgtgt
      481 ttggattgcg atggcacttg cccaagatac ggataccatt ttcttagatg aaccaacaac
      541 ctatcttgat ctcaatcatc aattggaagt cttagaactt ttaaaagaac tgaatcaaag
      601 ccgacagaaa accattgtga tggtccttca cgatgtcaat ttatcagctc gattttcaga
      661 ttatatgatt gccatgaaag agggagatat tcgctaccat gggtctgtgt ccaatatgat
      721 gacaactgag atactgaggg acatttttag cattgaacct caattgatgc aggttgctgg
      781 tcaagattac cctattttgc taacttacga tttaaaaaat aataaggaga aaaaatgaaa
      841 aagtttttag ctatttttag tttatttgtt gggctgattt tcttaacagc ttgttctacc
      901 tcttcctct
//
LOCUS       DBNFEG010000270          906 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.272, whole genome shotgun sequence.
ACCESSION   DBNFEG010000270 DBNFEG010000000
VERSION     DBNFEG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.272"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            3..218
                     /locus_tag="AC1PNG_002095"
     CDS             3..218
                     /locus_tag="AC1PNG_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018167072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HIP6702552.1"
                     /translation="MAEFTFEIEEKLLVLSENDKGWTKELNRVSFNGAPAKYDIRTWS
                     PDHTKMGKGITLSNEEFQVLLDAFANK"
     gene            complement(230..769)
                     /locus_tag="AC1PNG_002096"
     CDS             complement(230..769)
                     /locus_tag="AC1PNG_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIP6702553.1"
                     /translation="MKTTSIKSLVYIAIGTALISTLSQISLSIGPVPFTLQTLAIGLV
                     ACLYKPKEAITSVSLYLVLGAIGLPVFSGFSGGFAALTGPTSGFLWGFLLYTIITSAL
                     TKSDSSPVTIFLACLLGTSICFLLGCFVFKLVLGASWTDSIGWTVLPFILPDLAKITL
                     VMICHRLLQPVIKKEAYFA"
BASE COUNT          331 a          145 c          197 g          233 t
ORIGIN      
        1 aaatggcaga atttacattt gaaattgaag aaaaattatt ggtcttatct gaaaatgata
       61 aaggatggac caaggagctt aatcgagtat cttttaatgg cgcaccagcc aagtatgaca
      121 tccgtacctg gagtcctgac cataccaaga tggggaaagg aatcacactt agtaatgagg
      181 aatttcaagt tctactcgat gcgtttgcca ataaataaac aaaggaagcc taagcaaaat
      241 aggcttcttt tttgataaca ggttgcaata aacgatgaca aatcatgact agtgtaattt
      301 tagctaaatc aggaagaatg aaaggcagaa cagtccatcc gatagagtca gtccaactgg
      361 cacctaaaac tagcttaaat acaaaacagc cgagaaggaa acagatactt gttccaagta
      421 ggcaggctag aaaaattgtg actggtgagg agtctgattt agtgagggca gaagtgatga
      481 tagtgtagag tagaaacccc catagaaagc ccgaggtcgg tcctgtgagt gcagcaaagc
      541 caccagaaaa accagaaaaa acaggtaaac cgatagcacc aagaactaga tataagctga
      601 cacttgtaat tgcctccttg ggtttgtata gacaagctac taagccgata gctagggttt
      661 gtaatgtaaa tggtacaggt ccgatagaca gactgatttg tgaaagggtg gaaattaagg
      721 ctgttccaat agcgatataa acaagtgatt tgatagaagt tgttttcatt tgttatcctc
      781 ttaaaatttt atggttaaca aaaattatag tgagatttga taagaaagtc aatgctctct
      841 catcggctgt aacaactcta taggtgattt ttataacagc taataagaaa cttatatagt
      901 aaaaaa
//
LOCUS       DBNFEG010000271          906 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.273, whole genome shotgun sequence.
ACCESSION   DBNFEG010000271 DBNFEG010000000
VERSION     DBNFEG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.273"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..905)
                     /locus_tag="AC1PNG_002097"
     CDS             complement(<1..905)
                     /locus_tag="AC1PNG_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HIP6702554.1"
                     /translation="MRNFTTRLFILSGKYKLLFYILELTKNIAKFFWRIPKYIKRTLL
                     ALQRDKQRRNNYSDIKNRYLIYTIYEHQSSLQDYKVIFLEALAKISRDVLIVVNGKLP
                     QADINRLAQYGKVLERENEGYDVAAFRHGIIHTGKEALQQYNQLILVNDTNIGPFRDL
                     EEVFSEFNSDQLDFWGISMGEEQLDFTGYNPYGKIPKHLQSYFVVIENSLLRYEGFYD
                     YWEKLSDTDSRNKAIGKHETVFAKYFYDRGFKYDALIKDTKDSALYIHPLKLLKQGCP
                     LVKYSAFRNYDREQYFWHGLERESE"
BASE COUNT          305 a          176 c          121 g          304 t
ORIGIN      
        1 atctcggatt ctctttctaa accatgccaa aaatattgtt ctctgtcata atttctaaag
       61 gccgaatact taactaatgg acaaccttgc tttaataatt taagcggatg aatatacaat
      121 gcactatctt tcgtgtcttt aatcaatgca tcatacttaa agcctcgatc ataaaaatat
      181 tttgcaaaca cagtttcatg ttttccaatt gccttattac gagaatccgt atcagagagc
      241 ttttcccaat aatcataaaa tccttcatac ctaagcaagc tattttcaat aacaacaaaa
      301 tatgattgta agtgtttagg aattttacca taagggttat aaccagtaaa gtctagttgc
      361 tcctcaccca tggaaattcc ccaaaagtct agctgatcgg aattaaactc agagaaaacc
      421 tcttccaaat ctctaaaagg accaatatta gtatcattaa ccaagatgag ctgattatac
      481 tgttgtaacg cctcttttcc agtatgtatt atcccatgtc ggaaagccgc aacgtcatac
      541 ccctcatttt ctctttccaa aaccttacca tattgtgcta aacgatttat atcagcctgt
      601 ggaagtttac catttacaac aattaaaaca tctcgagaaa ttttagccaa tgcctctaaa
      661 aaaattactt tatagtcctg taaagacgat tgatgttcat aaattgtgta aatcaagtaa
      721 cgatttttga tatctgaata attatttcta cgttgcttat cacgttgcaa agctagtaga
      781 gtccgcttta tatattttgg aattctccag aaaaactttg caatattttt ggtaagctct
      841 aatatataaa acaatagctt gtatttacct gataagataa ataatcgcgt tgtgaaattt
      901 ctaatc
//
LOCUS       DBNFEG010000272          903 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.274, whole genome shotgun sequence.
ACCESSION   DBNFEG010000272 DBNFEG010000000
VERSION     DBNFEG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.274"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..899)
                     /locus_tag="AC1PNG_002098"
     CDS             complement(<1..899)
                     /locus_tag="AC1PNG_002098"
                     /inference="COORDINATES: protein motif:HMM:NF016903.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HIP6702555.1"
                     /translation="MIIPGTSFIPPIRVSQTTETVRSSTSVLLHVHIESVSIFEEYIE
                     ELCKISDRCQLLITLPETDFSNKCSIVERYLSTYKLRAQIAKLTDELHFFEIVNNYMG
                     DAKYLAHVTVKQTKEIKYSVEDIIDRHQLRKMFFTSFDAVISNFESQSNLAVVIPDLT
                     TNQRYDRQSLREGNPELIRQLNILYESLVRTKKVDFYKVPYIIGEEVSWYWIKTEDYK
                     KIEEKFRNIDFSKEDRLLLVPILFIYSAWDLSNDYAVVENTENVSPILEKISFSSERE
                     LRLIIEEKEFLQIGLRRTLKIISV"
BASE COUNT          289 a          173 c          117 g          324 t
ORIGIN      
        1 actgatatta tctttagtgt ccttctcagt ccaatttgta aaaattcctt ttcttcaata
       61 attaaacgca attctctttc gctagaaaaa ctaatctttt ccaatattgg actcacattt
      121 tcagtattct cgaccactgc ataatcgttt gataaatccc acgcgctata aataaataat
      181 attggaacta gcaataatct atcttctttt gaaaaatcaa tgtttctaaa tttctcctca
      241 atctttttat agtcttctgt ttttatccaa taccaactta cttcttctcc aattatataa
      301 ggcactttat aaaaatcaac tttcttagtt ctcacaagtg attcataaag tatgttcaat
      361 tgacgaataa gttctggatt gccctctcga agactttgtc tatcatatct ttgattagtt
      421 gttaagtctg gaataactac agccaaatta gattgtgatt caaaattaga aatcactgca
      481 tcaaatgatg taaaaaacat tttcctcaac tgatgcctgt caataatatc ctctacagaa
      541 tattttattt cctttgtttg tttgacagtt acatgagcaa gatatttggc atctcccata
      601 tagttgttta ctatttcaaa aaaatgcaat tcatctgtga gttttgcaat ttgggctcgc
      661 aatttgtaag tagacaaata tctctcaact atactacact tatttgaaaa atcagtttct
      721 ggcaatgtta ttagtaactg acacctatca gatattttgc ataactcttc aatatactct
      781 tcaaaaatag acactgattc tatgtgaaca tgaagaagta ccgacgtcga agaacgaacg
      841 gtctccgtcg tttgcgaaac ccgtattgga ggaataaaac tcgtacccgg aataattatc
      901 tgc
//
LOCUS       DBNFEG010000273          876 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.275, whole genome shotgun sequence.
ACCESSION   DBNFEG010000273 DBNFEG010000000
VERSION     DBNFEG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.275"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>876
                     /locus_tag="AC1PNG_002099"
     CDS             <1..>876
                     /locus_tag="AC1PNG_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HIP6702556.1"
                     /translation="TGIKKKINENKIADKGKTFEELVEEAKIAVGKGGPLIIDVVNSI
                     PELLAFYDEIKTCSFEEVSNKSKVNKESLLAYKFNGLSSRYEAGTDRDRILKRLDLIY
                     ELLELYKTGKHNEFLRITKFNITSSEDKISLSKVMTKISVDDITIGKVIELAEESGLI
                     SKDDLFTNFIESRGHYLWSRLKIMPFQEYVNSIAYLREYVSVITQHKVKGSEYDNVLV
                     LLDNGKWNQYNFDSLFGKGSVNENVQKRTKKLFYVAITRAMKNLIVYMPSNDSQIIQK
                     AQDYFEPIDIIDVLS"
BASE COUNT          337 a           86 c          169 g          284 t
ORIGIN      
        1 ttacaggtat aaagaaaaaa atcaatgaaa ataagatagc tgataaagga aaaacgtttg
       61 aagaactggt agaagaggct aaaatagctg tcggaaaggg aggtccatta ataattgatg
      121 tggttaattc catcccagaa cttttggctt tttacgatga aattaagacg tgctcgtttg
      181 aagaagttag taataagagt aaagttaata aagaatcgct gttagcttat aaatttaatg
      241 gattaagtag tagatatgaa gcgggcacag atagggatag gattcttaaa aggttagatt
      301 taatctatga gctattagag ctgtataaaa caggtaagca taatgaattc ttacgtatta
      361 ctaaatttaa tataacttca tcagaagata aaattagtct atctaaagtt atgacaaaaa
      421 tctctgttga tgatattaca attggaaaag tcatagaact tgcagaggaa agtggattaa
      481 tttcaaagga tgacttattt actaatttta ttgaaagtag gggacattat ttatggtcac
      541 gacttaagat aatgccattt caagaatatg taaatagtat tgcttatctc agagaatatg
      601 tatctgttat aacgcagcat aaggttaaag gtagtgaata tgataatgtt ttagttttat
      661 tagataatgg aaaatggaat caatataatt ttgatagcct atttggtaaa ggttcagtta
      721 atgagaatgt ccaaaaaaga actaagaagt tattctatgt tgcaattact agagcaatga
      781 aaaatttaat tgtttacatg ccgagtaatg atagtcagat tattcaaaaa gcacaagatt
      841 attttgaacc aattgatatt attgatgttt tgtctt
//
LOCUS       DBNFEG010000274          857 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.276, whole genome shotgun sequence.
ACCESSION   DBNFEG010000274 DBNFEG010000000
VERSION     DBNFEG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.276"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            152..>857
                     /locus_tag="AC1PNG_002100"
     CDS             152..>857
                     /locus_tag="AC1PNG_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HIP6702557.1"
                     /translation="MKTLYHYTTVETLLLILNYKTLRFKSLLYVDDPLEPSTSDFGNL
                     GGFKYVSCWTDTPNSIPQWTMYSDNMTGVCIGISFDKETDVFLTEKFSLSESSEPIDM
                     VNALHPLKSGLLVTNNKYVPSLEQIRYTDDVSLITPRVVSSDDKFTTINLASNGIYKT
                     TDWSFQNEQRFSFQIFPLPIDLVLELMNANKGDLTGIINSFISVKPKEYFDLDLNPTI
                     FSNMTITFGKRCSAEDK"
BASE COUNT          288 a          114 c          142 g          313 t
ORIGIN      
        1 tcgaccccgg ccgccggtat agtttaaaag acaaggtttc taagccttgt ctttttgttt
       61 tgaggttata aaattttcag ttgagttact aaaaattact taatgttttg ttgtataata
      121 tgttatataa gcaagaggag gagccgttaa tatgaagaca ctttatcatt atacaacagt
      181 tgaaacattg cttctgatat taaattataa aacattgaga ttcaaaagtt tactttatgt
      241 tgacgatcca ttagaacctt caacttcaga ctttggtaat ctaggtggat ttaagtatgt
      301 gtcttgttgg actgatactc ctaatagtat tccacaatgg acaatgtatt ctgataatat
      361 gacaggagtg tgtataggaa ttagttttga caaagaaact gatgtctttt taacggaaaa
      421 attttcttta tctgaatctt ctgaaccaat tgatatggta aatgcattac atcctttaaa
      481 atcaggctta ttagttacaa ataataaata tgtgccttct ttggaacaaa taaggtatac
      541 tgatgatgtt tcattgatca ctcctcgagt agtttcttca gatgataaat tcacaactat
      601 taatttagct tcaaatggta tctataagac aactgattgg agttttcaaa atgagcaaag
      661 attttctttt caaatttttc cattaccaat agatttagtt ttggaattaa tgaatgcaaa
      721 taaaggagat ttgactggaa taattaatag ttttatttct gttaaaccta aagaatactt
      781 tgatttagat ttaaatccga ccatattcag taatatgaca attacgtttg gtaaaagatg
      841 ctctgctgaa gataagt
//
LOCUS       DBNFEG010000275          852 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.277, whole genome shotgun sequence.
ACCESSION   DBNFEG010000275 DBNFEG010000000
VERSION     DBNFEG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.277"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            41..850
                     /locus_tag="AC1PNG_002101"
     CDS             41..850
                     /locus_tag="AC1PNG_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIP6702558.1"
                     /translation="MKKYIMTGLVLLATIGLSACSGNATQEDTSLKDVQEKGKLIVAL
                     NPEFPPFEFRTLVDGKDTIVGADIELAKAIGQELGVEVEFSAMSFDNVLNNVQSGQSD
                     IAISGISATEERAKVYDFSIPYYTSTNKVIINKEDLAQYTSLDSLAEANIGAQKGSIQ
                     EQIIKEQLPSSTVIALASNGELINQLKSQKVEAVIFEEPIAKAYVAKNDDLVILDTEI
                     KSSYSDAYAIALPKGSTALKEKVDSVITKLVESGQMDQFVQEAYELSIANN"
BASE COUNT          297 a          133 c          173 g          249 t
ORIGIN      
        1 tataagtatg cacaggatta ttttaaagga gaatatgatg atgaaaaaat atattatgac
       61 aggcttggtt ttactggcaa caataggtct aagtgcttgt agtggaaatg ctactcaaga
      121 agatacttcc ttgaaggatg ttcaggaaaa gggaaaacta attgttgcat taaatccaga
      181 atttccacca tttgagttta gaacactggt tgatggaaaa gatacaatcg ttggagctga
      241 cattgagctt gcaaaagcca ttggtcaaga attgggtgta gaagtcgaat tttcagcgat
      301 gagttttgat aatgttttga acaatgtcca gtcagggcaa tcggatattg ccatttcagg
      361 aatttcagcc actgaagagc gtgcaaaagt gtatgatttt tccattcctt actacacctc
      421 aaccaataaa gttattataa ataaagagga tcttgcacaa tatacatcgc ttgattctct
      481 agctgaggca aatataggag cacaaaaagg ttctattcaa gaacaaatta ttaaagaaca
      541 gcttccatcc tccacggtta ttgctctagc ttcgaacgga gagctgatta atcaattaaa
      601 atcacagaag gtagaagcag ttatttttga agagccgatt gcgaaagcct atgttgccaa
      661 aaatgatgat ttagttattt tagatactga gataaaaagt agctattcag acgcctatgc
      721 tattgccttg ccaaaaggaa gtacagcttt aaaggaaaaa gttgactctg tcattacaaa
      781 actagtggaa tctggacaga tggatcaatt tgttcaagaa gcctatgaat tatccattgc
      841 taacaattaa at
//
LOCUS       DBNFEG010000276          849 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.278, whole genome shotgun sequence.
ACCESSION   DBNFEG010000276 DBNFEG010000000
VERSION     DBNFEG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.278"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..>849)
                     /locus_tag="AC1PNG_002102"
     CDS             complement(<1..>849)
                     /locus_tag="AC1PNG_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HIP6702559.1"
                     /translation="HKIIERKSLTVSSVMNGLSGLAGIAVYIMTDLNALLLDGAFSLI
                     AFISSLVAVYISKNSHRKTETFPQGLYFLEPLYAIMKSLATLLLLVFAVLETSATAFA
                     YFVHGIGHQMTTGPVLPYTITMFFVCMGLYLYNRHMSTKVNHLSTIILAEAKGNLVDG
                     LISGAIGIAILLLYLVPINSWFGFLHYTGDFFLTLALAIISFKEPWNLLVASFKELAN
                     GTIHFPEIHDSIYQLLEPYLKEDSNDVEIHIFKQGMKIRVKINLHNIHHEIIQKLLED
                     KPQMLY"
BASE COUNT          271 a          134 c          202 g          242 t
ORIGIN      
        1 gatagagcat ctgaggctta tcttctaaga gtttttggat aatttcgtga tggatattgt
       61 ggagattgat tttaacgcga atcttcatgc cttgtttgaa aatatggatt tcgacatcat
      121 tgctatcttc ctttaagtaa ggctccaata gctgatagat agaatcatgt atctcaggga
      181 aatggattgt tccgttggca agttccttga aagatgccac taataagttc caaggttcct
      241 taaatgaaat gattgctagt gctagtgtca aaaagaagtc accagtataa tgcaggaagc
      301 cgaaccaaga gttgatagga acaagataga gcagaagaat tgcaattcca attgcacctg
      361 aaatgagtcc gtctacaagg ttgccttttg cttctgctaa tatgatagta gacagatgat
      421 tgactttggt actcatatgg cggttgtata ggtaaagccc catacagaca aaaaacatgg
      481 ttatggtata agggagaacg ggacctgtag tcatttgatg accgattcca tgaacaaagt
      541 aagcaaaggc tgtcgcgcta gtttcaagta cagcaaatac aaggagtagt agagttgcga
      601 gagatttcat gatagcgtag agaggttcta gaaaatacag tccttgagga aaagtctcgg
      661 ttttccgatg gctatttttt gatatgtaaa ctgcaactaa ggacgaaata aatgctatta
      721 gagaaaaggc tccgtctagg aggagggcgt ttaggtcagt catgatataa acggcaatac
      781 ctgctagtcc agataaaccg ttcataacag aggaaacagt tagagatttg cgttcgataa
      841 ttttgtggt
//
LOCUS       DBNFEG010000277          828 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.279, whole genome shotgun sequence.
ACCESSION   DBNFEG010000277 DBNFEG010000000
VERSION     DBNFEG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.279"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>828
                     /gene="coaA"
                     /locus_tag="AC1PNG_002103"
     CDS             <1..>828
                     /gene="coaA"
                     /locus_tag="AC1PNG_002103"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009568566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HIP6702560.1"
                     /translation="FLNFEQIDRATWQQLHRKTTIPLSQSELNSIKSFNDRIQLHEVS
                     DIYLPLVNLIHIYRKARKDLNFTKSLFLQKTIKPQPFIIGVSGSVAVGKSTTSRLLQI
                     LIARTFKHAKVELVTTDGFLHPNAVLEERQLLNKKGFPESYDMEKLIDFLDKIKNGYD
                     CQIPVYSHEIYDIVPNKTQEIKSPDFLIVEGINVFQNPQNQRLYVSDYFDLSIYVDAD
                     VEHIETWYLERFQKLLTLAKNDPNNYYHRFTQMTYPEILSIAQNTWKNINLANLEKFI
                     "
BASE COUNT          279 a          169 c          130 g          250 t
ORIGIN      
        1 tttttaaatt ttgaacaaat tgaccgagcc acttggcaac aactacatcg taagactacc
       61 attccactta gtcaaagcga actaaattct attaaaagtt ttaacgaccg catccaatta
      121 catgaggttt ctgacatcta tcttcctttg gttaatctta tccatatcta tcggaaagca
      181 cgcaaagacc taaattttac gaagagttta tttctacaga agactatcaa gccacaacca
      241 tttatcattg gcgtttctgg aagtgttgct gtcggcaaat caacaactag tagattgctg
      301 caaattctta tcgctcggac atttaaacac gccaaggttg aattagtaac aacagatggt
      361 tttctccatc caaatgcggt cttagaagaa cggcaactgt taaataaaaa aggttttcct
      421 gaatcctatg acatggagaa attgattgat ttccttgata agataaaaaa cggctacgac
      481 tgccaaattc ctgtctattc acatgaaatc tatgatattg tccccaacaa aactcaggaa
      541 attaaatctc cagatttctt gattgttgaa ggaatcaatg tatttcaaaa tccacaaaac
      601 cagcgcctct atgtcagtga ctattttgac ctatctatat acgttgacgc tgatgtggag
      661 catattgaaa cctggtactt ggagcgattc caaaaattat tgacattggc aaaaaatgac
      721 cctaacaact attaccatcg ttttacccag atgacgtacc ctgagattct atctattgcc
      781 cagaatactt ggaaaaatat taatctagct aacttagaaa aattcatt
//
LOCUS       DBNFEG010000278          807 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.280, whole genome shotgun sequence.
ACCESSION   DBNFEG010000278 DBNFEG010000000
VERSION     DBNFEG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.280"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>807
                     /locus_tag="AC1PNG_002104"
     CDS             <1..>807
                     /locus_tag="AC1PNG_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIP6702561.1"
                     /translation="AFLKKLSSIDAPLQSGEKYAETSHILGSEEHKVRLVVKGNSVTV
                     SDNGIPLLSYNSPNNWNDAQASIVFSPVSNRSVSLDDIIVRRTQTLRPFRVVSRLDGQ
                     EVSGVLPGSIKGTTSQVFVGDSLNLEVIEKSGYQFIGFKDSAGNFLDLTTFVVPNEES
                     ELVLYADFESVDVVIREPKTFYIDSLGGNDSNNGETENSAWKSFEQLRKKSFVAGDRI
                     LIKRGSRFVGESAFLTFKGSGSQNTPILISSYGEGELPLLEGQGKIENVIK"
BASE COUNT          262 a          112 c          174 g          259 t
ORIGIN      
        1 gcttttttaa aaaaactttc tagtatagat gcaccacttc aatctggcga aaaatatgct
       61 gaaacaagtc atattttagg aagtgaagag cataaagtac gacttgtagt caaaggtaat
      121 tcagtaacag ttagtgataa tggaatacca ttattaagtt acaacagccc aaacaattgg
      181 aatgatgccc aagcatctat tgttttttca ccagtttcaa atcggagtgt ttctctagat
      241 gatattattg ttcgaagaac tcaaacctta agacctttcc gggtcgtttc tcgattagat
      301 ggtcaggagg tatctggagt tctgcctggt tcaattaaag gaaccacaag tcaagttttt
      361 gttggagatt cacttaattt agaggtcatt gaaaaatctg ggtaccaatt tattggtttt
      421 aaagatagtg ctggtaattt tcttgattta actactttcg ttgttccaaa tgaggagtca
      481 gagttagtgc tgtatgccga ttttgagtca gtggatgtag tgattcgtga accaaagact
      541 ttctacattg atagtctagg aggaaatgat tctaataacg gggaaaccga aaatagtgca
      601 tggaaatcat ttgaacaatt aaggaaaaag tcgtttgtag ctggagatag aatattaata
      661 aaacgtggaa gtcggtttgt tggagaaagt gcttttctta catttaaagg ttcgggtagt
      721 caaaatactc caattctaat ttcatcctat ggtgaaggtg agttaccttt attagaaggg
      781 caagggaaaa ttgaaaatgt aattaag
//
LOCUS       DBNFEG010000279          787 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.281, whole genome shotgun sequence.
ACCESSION   DBNFEG010000279 DBNFEG010000000
VERSION     DBNFEG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.281"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            21..782
                     /locus_tag="AC1PNG_002105"
     CDS             21..782
                     /locus_tag="AC1PNG_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIP6702562.1"
                     /translation="MIKKERHERILDMLNIEGVITVKDMMKELNISDMTARRDLDALA
                     EAGFLTRIHGGAQRLYKDEEPHEKTHIEKKVLQTKEKKLIAQKANSIIKDGETVFIGP
                     GTTLEHLAVELKTRNIRVITNSLPVFLILNKSRTVDLLLIGGEYREITGAFVGSMATT
                     NLKTLRFSKAFVSANAVSDNAIATYSDVEGEIQQLALDNAVEKILLVDSTKFNRYDFF
                     NFYTLEQIDTIITDNQISQDQLNEFGQLTTIIQAN"
BASE COUNT          294 a          128 c          140 g          225 t
ORIGIN      
        1 tacagaaagg aaacatagtt atgataaaga aagaaaggca cgaacgtatc ttagatatgt
       61 taaatattga aggtgttatt actgtcaaag atatgatgaa agagctaaat atctctgata
      121 tgactgctag aagagattta gacgcacttg ctgaagcagg attcctgact cgtatacatg
      181 gaggagcaca acgactctat aaagatgaag aaccgcacga aaaaactcat attgaaaaaa
      241 aggtacttca aactaaagag aagaaactta ttgcacaaaa agccaattcg atcatcaaag
      301 atggtgaaac tgtttttatt ggtcctggaa caacgttaga acatctagca gttgagttaa
      361 aaactcgtaa tattcgtgta attacaaaca gtcttcctgt tttccttata ctgaataaga
      421 gtaggactgt tgacttatta ttgattggcg gagagtacag agaaattaca ggtgctttcg
      481 ttggttcaat ggctacaacc aacttaaaaa ccttacgttt ttctaaagca tttgtaagtg
      541 ctaacgccgt atcagataat gctatcgcaa catacagtga tgttgaagga gaaattcaac
      601 aattagcact ggacaatgct gttgaaaaaa tcttgttggt tgatagtacc aaattcaatc
      661 gttatgattt ctttaatttc tatactctag aacaaattga taccatcatc accgataatc
      721 aaatatccca agaccaatta aatgaatttg gccaactaac aactattatt caagctaatt
      781 aaactaa
//
LOCUS       DBNFEG010000280          780 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.282, whole genome shotgun sequence.
ACCESSION   DBNFEG010000280 DBNFEG010000000
VERSION     DBNFEG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.282"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            21..548
                     /locus_tag="AC1PNG_002106"
                     /pseudo
     CDS             21..548
                     /locus_tag="AC1PNG_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636114.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          230 a          142 c          182 g          226 t
ORIGIN      
        1 agaattggtg gaggataaaa atggaacaat tcaatcaaga acagttatat ggagtggtta
       61 ttcaaaccat gacagctgct agtcaacttc ctatggttaa agtcaatcgg gaagaatttt
      121 tgaagactcg ttttaaagat agtccatatc tagataaaat tattgcggat ggaccaacag
      181 cggtcttcac tacaaaagct ttgcgtaagg aagctgaaaa aatcattaat gatatgacta
      241 cgaaaacatc tttgatttct ttcgcagctg gtttaccggg gaatccattt acagcggtgg
      301 cggcaggtac tgctgatata gctcagtatt ttggttttgc cttgaatttg tcgcaacaaa
      361 tcgcttatct ttttggtgaa gatgaattat ttgttggtga agggaaggaa atttcagaag
      421 aggttcaaat ccgcattatt gcctacattg gtgtcatgtt tggagcaggt ggttcagcgg
      481 ttcttctcaa taaagtatcc aagacagtcg gagctaatat cggtaaaaaa gtagctatgc
      541 aggctttgta ggctgggcaa aaagaccaac ctcgcttctc agggttcgtg tcaacatctc
      601 agcaattcgt gttttacact ccaaatctgg cctctatgac tgatgtgaac agagttcgct
      661 tcatctccaa cctcaaactg tctcccagac agtttgagct gtgcgggggt gggagtgaaa
      721 cagtctgggg atagactgtt tcagctcaac aactagaaat aaagacttgt tgacgaactc
//
LOCUS       DBNFEG010000281          773 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.283, whole genome shotgun sequence.
ACCESSION   DBNFEG010000281 DBNFEG010000000
VERSION     DBNFEG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.283"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(60..311)
                     /locus_tag="AC1PNG_002107"
     CDS             complement(60..311)
                     /locus_tag="AC1PNG_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702563.1"
                     /translation="MKIYKLLFYITYPALVFSIYLLLDMTSEYGTPLIETVFVAGGFL
                     VIPLLLWSLDIISVESVKEESVDNEKNNKASVTMGQTEA"
     gene            complement(367..690)
                     /locus_tag="AC1PNG_002108"
     CDS             complement(367..690)
                     /locus_tag="AC1PNG_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702564.1"
                     /translation="MKTSKDIFLSEGMKMPNINGIKRVQSFNSDVSVNFLLDDESRDF
                     LKENLPLTGVVIYEPTLKKLAENIIILNRQKHRMSDESRISLMNKEIYQGYREASFYT
                     SIIEA"
BASE COUNT          241 a          143 c          116 g          273 t
ORIGIN      
        1 aagacttaga aataatactg ctataaataa ctggtatatg taagttaggt ctaactgttt
       61 taagcctctg tttgtcccat cgtaacactc gccttattat tcttctcatt atcaacactc
      121 tcttccttca cgctttcaac tgatataatg tccaacgacc ataacaaaag tgggataact
      181 aagaaccccc cagcaacaaa aaccgtttct attaatggag ttccgtattc gcttgtcata
      241 tctaatagca agtaaataga aaagactaac gctggataag ttatataaaa caatagtttg
      301 tagattttca tgaaatttgt cctttcttaa aacaaaaggg ggaggggtta gctcctcctt
      361 atatcactaa gcttcaatta ttgaggtgta aaaagaagct tccctgtaac cttggtaaat
      421 ctctttgttc atcaagctaa tcctagattc atcagacata cggtgttttt gacgattaag
      481 aataatgatg ttttctgcta acttcttcaa ggttggctca tagattacta ctccagtaag
      541 tggcaaattt tcctttaaaa aatctctgga ttcatcatct aagagaaagt ttaccgatac
      601 atctgaatta aagctttgta cccgcttaat cccattgata ttcggcattt tcattccttc
      661 gctaaggaaa atatctttag atgttttcat ctatttattt ctccattttt taatttgtta
      721 taatcaatat ttttaatgcg gtaagtattg attggtgaag ctctagaatt cat
//
LOCUS       DBNFEG010000282          756 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.284, whole genome shotgun sequence.
ACCESSION   DBNFEG010000282 DBNFEG010000000
VERSION     DBNFEG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.284"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>756
                     /locus_tag="AC1PNG_002109"
     CDS             <1..>756
                     /locus_tag="AC1PNG_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP6702565.1"
                     /translation="LHDIITGINSGIFKEEGLEVELIEFADGPTIIASLESGSIDIGN
                     IGPGAHKLAIEGRAEVVAFSQLGNADELIARSDRGIDSIKDLRGKKIGTASGTSAETI
                     LKLALLDANLSEKDVEIIDMDASAIVTAMLSGSIDVAATWSPNTTLIKRELKEKVVVL
                     ADNSRYRDQSPAIASYVTFPGYSEKNNEKISKFLKGLYRAMDYRAEHMKEVSKWVATQ
                     IGSDITAVQDQLQDGEWLTSRQMIQLINTGILQ"
BASE COUNT          250 a          108 c          171 g          227 t
ORIGIN      
        1 gtcttcatga cattattact ggaataaatt caggtatctt taaagaggag gggcttgaag
       61 tcgaattgat tgaatttgct gatggtccaa ctatcattgc atctttagag tcaggttcaa
      121 ttgatattgg taatattggt ccgggtgccc ataaattggc gattgaaggt agagcagaag
      181 ttgttgcatt tagtcaactt gggaatgctg acgaattgat tgctcggtcg gatagaggaa
      241 ttgattcaat aaaggatttg aggggaaaga aaattggaac agcatctggg acatcagcag
      301 aaacaattct aaagcttgct ttgttagatg caaatttgtc tgaaaaagat gtagaaatta
      361 ttgatatgga tgcctccgct attgttacag cgatgttgtc gggatcaata gatgtagctg
      421 caacttggtc acctaataca actttaataa agcgagaatt aaaggaaaaa gttgttgtat
      481 tagctgataa ttcacgttat agagaccaat ctccagctat tgcttcatat gtaacttttc
      541 cggggtattc tgaaaaaaac aatgaaaaaa tttccaagtt tttaaaaggg ttatatcgtg
      601 caatggatta tcgagctgaa catatgaagg aagtttcaaa atgggttgct acccaaatcg
      661 gttcagatat cacagctgtt caagatcaac tacaagatgg agagtggtta acaagtagac
      721 aaatgattca attgattaac actgggatac ttcaac
//
LOCUS       DBNFEG010000283          743 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.285, whole genome shotgun sequence.
ACCESSION   DBNFEG010000283 DBNFEG010000000
VERSION     DBNFEG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.285"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..580
                     /locus_tag="AC1PNG_002110"
     CDS             <1..580
                     /locus_tag="AC1PNG_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HIP6702566.1"
                     /translation="IRHDFASIISSLEPAIQEKNIDEVSIIYKEVLIKMNRNLQKNEY
                     SAFNLKNIEDLAIRNMLAQSILEAEQYDIQFNCYVSGHISQVNVPMLEMIQILSIMLT
                     NSIESAIISKKPLIEVALYRDKSIVTIVIKNSRNPISLDKRTIWEVGVSSKGEGRGIG
                     LYNLRRLVNRHDNLTLETQIDYDSFTHILKIM"
BASE COUNT          270 a           94 c          140 g          239 t
ORIGIN      
        1 gattcgccat gatttcgcta gcattatatc aagtttagag cctgcgattc aggaaaaaaa
       61 tattgatgaa gtaagcatta tctataaaga agttctcatt aagatgaata gaaatctaca
      121 aaagaacgaa tactcagcat ttaatttgaa aaatattgaa gacttagcta ttcgtaatat
      181 gctggctcaa tcaattttgg aagcagaaca atatgatata caattcaatt gttacgtgtc
      241 tggacatata tctcaagtca atgtaccaat gttagagatg attcaaatat tatctattat
      301 gttgactaat tcaattgaat ctgctattat ttcaaaaaaa cctttaattg aagtggcttt
      361 atatagagat aagagcattg ttacaatagt tattaagaat agtcgaaatc ccatctcttt
      421 agataagaga actatctggg aggtaggggt ttcaagtaaa ggtgagggga ggggaattgg
      481 gttatataac ttgagaagac tggtaaatag gcatgataat ttgactctag aaacacaaat
      541 agattatgat agttttactc acatcttgaa aataatgtaa gctggaggta tgttatgaat
      601 attattgttc tggatgacaa tattcatcat cagttgcgac tagaatctgc tctttatgat
      661 gtagctcggt ctttacatat tcatatcaat attgaatgtg ctagaacaat acaggcatta
      721 agagagtata tgaatcaaga aga
//
LOCUS       DBNFEG010000284          743 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.286, whole genome shotgun sequence.
ACCESSION   DBNFEG010000284 DBNFEG010000000
VERSION     DBNFEG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.286"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..406)
                     /locus_tag="AC1PNG_002111"
     CDS             complement(<1..406)
                     /locus_tag="AC1PNG_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702567.1"
                     /translation="MTYTLEWLETFDGEIDILNVDMSCLANTIEKYVSQYKYVYQTNM
                     ENYPEIVLAVPNSSIPHLLGLSRDHHVNLPTNNAGSIFEGLKDDWTLQRLNKADNGWF
                     NENKFKIVGTLLLYQMLHVMECKFYTTDGILNR"
BASE COUNT          228 a          158 c          111 g          246 t
ORIGIN      
        1 ttctattcaa aataccatcc gttgtataaa atttacattc catcacatgt aacatttgat
       61 aaagtagtaa tgtgccaacg attttgaact tattttcgtt aaaccatcca ttatcagcct
      121 tatttagacg ctgcaatgtc caatcatcct ttaatccttc aaaaatactc cctgcattat
      181 tagttggtaa attgacatga tgatctcttg ataatcctaa tagatgggga atagaggaat
      241 ttgggacggc gagaacaatt tcaggatagt tctccatatt tgtctgataa acgtatttgt
      301 actgagaaac atatttctca atagtattgg ccaaacaaga catatcgaca ttgagaatat
      361 caatctctcc atcaaaggtc tcgagccatt ccaaagtata tgtcatttta tttttctcct
      421 aaaaaagaga aggaagcagg tgcttccttc tcttccacgt attgagtttc acctcacgct
      481 agatatgccc tatctattcc tcaagggtag gcagtcctgc tattcttttt ctcaacaaac
      541 agtttgaact gagtgcggag cccacaggcg ggtgcagacc atatccacta gcccacaagt
      601 aatttataat ctttatttac ccttattata cgggataact ccttatttgt caacacttac
      661 gttgactctc actagtccct acgcatgaat aaaatacatg taactacttt ctagatacat
      721 gcattttctt caaaaatatt ttc
//
LOCUS       DBNFEG010000285          736 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.287, whole genome shotgun sequence.
ACCESSION   DBNFEG010000285 DBNFEG010000000
VERSION     DBNFEG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.287"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..>736)
                     /gene="tilS"
                     /locus_tag="AC1PNG_002112"
     CDS             complement(<1..>736)
                     /gene="tilS"
                     /locus_tag="AC1PNG_002112"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIP6702568.1"
                     /translation="GLDSMNLFHLLYECKQVLGIELGIAHINHGQREESVIEEKYLRQ
                     LAEESNVPFYLSYFEGVFSEEAARKWRYGFFATIMEKEGYTALVTAHHADDQAETVFM
                     RLIRGGRLRHLSAIQPTQPFATGELIRPLLSFKKADFEKLFHFEDCSNADFKYFRNRV
                     RNDYLPKLKQENPKIEFTLNNFAVDTNNLFQALRDLTQDLSVTDVMSFQQQTPAVQSY
                     LLEEYLEKFPDLQLSRPQFNEVLHILR"
BASE COUNT          227 a          139 c          125 g          245 t
ORIGIN      
        1 aacgcaagat gtgtaatact tcattaaatt gtggacgaga gagctgtaaa tcaggaaatt
       61 tttccaaata ttcctctaat agataacttt gtaccgcagg tgtctgttgt tgaaaactca
      121 tcacatctgt aacggataaa tcctgtgtca aatctcttag cgcttgaaat agattatttg
      181 tatcaactgc aaaattattg agagtaaatt cgattttagg attttcctgt tttaactttg
      241 gtaaatagtc atttctgact ctatttcgaa aatatttgaa atctgcattg ctgcaatctt
      301 caaaatgaaa tagcttttca aaatctgcct ttttaaaaga taaaagtggt cgaataagtt
      361 ctcctgttgc aaaaggctga gtcggttgaa ttgctgataa gtgacgtaaa cgccctcctc
      421 taatcaatcg cataaagaca gtttctgctt gatcatctgc atggtgggct gtcactagtg
      481 cagtgtagcc ttccttctcc atgattgtag caaaaaatcc ataacgccat tttcgcgctg
      541 cttcttctga aaaaacacct tcaaaatagg acagataaaa gggaacatta ctttcttctg
      601 ctaactgtct aagatatttt tcctcaatga cagattcttc tctctgtcca tgattaatat
      661 gggcaattcc aagttcaatc cctaacactt gtttgcattc gtataaaaga tggaataggt
      721 tcatagaatc taaccc
//
LOCUS       DBNFEG010000286          733 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.288, whole genome shotgun sequence.
ACCESSION   DBNFEG010000286 DBNFEG010000000
VERSION     DBNFEG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.288"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(105..178)
                     /locus_tag="AC1PNG_002113"
     tRNA            complement(105..178)
                     /locus_tag="AC1PNG_002113"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(142..144),aa:Arg,seq:acg)
     gene            complement(197..280)
                     /locus_tag="AC1PNG_002114"
     tRNA            complement(197..280)
                     /locus_tag="AC1PNG_002114"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(244..246),aa:Leu,seq:taa)
     gene            complement(288..359)
                     /locus_tag="AC1PNG_002115"
     tRNA            complement(288..359)
                     /locus_tag="AC1PNG_002115"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Gly,seq:gcc)
     gene            complement(372..444)
                     /locus_tag="AC1PNG_002116"
     tRNA            complement(372..444)
                     /locus_tag="AC1PNG_002116"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(409..411),aa:Thr,seq:tgt)
     gene            complement(459..540)
                     /locus_tag="AC1PNG_002117"
     tRNA            complement(459..540)
                     /locus_tag="AC1PNG_002117"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(504..506),aa:Leu,seq:tag)
     gene            complement(544..616)
                     /locus_tag="AC1PNG_002118"
     tRNA            complement(544..616)
                     /locus_tag="AC1PNG_002118"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(581..583),aa:Lys,seq:ttt)
     gene            complement(662..733)
                     /locus_tag="AC1PNG_002119"
     tRNA            complement(662..733)
                     /locus_tag="AC1PNG_002119"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(699..701),aa:Asp,seq:gtc)
BASE COUNT          189 a          216 c          154 g          174 t
ORIGIN      
        1 gaacctgcga caccttggtc ccaaaccaag tactctacca agctgagcta cttcccgata
       61 ctattaaaaa taaagctccc ctaacgtctc cattaagcga gtgatgcacc ctagaggatt
      121 cgaacctcta accgcctgat tcgtagtcag gtactctatc cagttgagct aagggtgcta
      181 actattataa tcttcatgcc gaggaccgga atcgaaccgg tacgatgttg ccatcgcagg
      241 attttaagtc ctgtgcgtct gccagttccg ccaccccggc ctctcaaagc gaacgacggg
      301 attcgaaccc gcgaccccca ccttggcaag gtggtgttct accactgaac tacgttcgca
      361 atctttccta atgccggcta catgacttga acacgcgacc ctctgattac aaatcagatg
      421 ctctaccaac tgagctaagc cggcctattt ctattctatg cgggttaagg gacttgaacc
      481 cccacgcccg aaagcgccag atcctaaatc tggtgcgtct gccaattccg ccaaacccgc
      541 atatgactcg tgctgggctc gaaccagcga cccattgatt aaaagtcaat tgctctacca
      601 actgagctaa cgagtcttta cttaattata ttacttgaaa catatgattg attcgtttca
      661 acggtcccga cgggaatcga acccgcgatc ttcgccgtga cagggcgacg tgataaccgc
      721 tacactacgg gac
//
LOCUS       DBNFEG010000287          731 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.289, whole genome shotgun sequence.
ACCESSION   DBNFEG010000287 DBNFEG010000000
VERSION     DBNFEG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.289"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(88..720)
                     /locus_tag="AC1PNG_002120"
     CDS             complement(88..720)
                     /locus_tag="AC1PNG_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HIP6702569.1"
                     /translation="MKEFKTIREDGIVEEEIKKSRFICFMKRVTSEEEARDFINKIKK
                     EHYKATHNCSAFVLGENMEIKRSSDDGEPSGTAGVPMLTVLENHELTNVATVVTRYFG
                     GIKLGAGGLIRAYAGVVANAVKEIGVVEVKEQEGISISMSYTQYQEFANWRAEQGLEE
                     FDAQFTTEVSTMIFVDKEFLEQTLASLTDFYHGKVSAEKANSRIVEVPVY"
BASE COUNT          202 a          170 c          138 g          221 t
ORIGIN      
        1 gtccagaggt cggggaatag tcggggagtt gtaactataa acagctatat aataagtaaa
       61 aaaaaactat ctcgccgata gcttttttta atagacaggt acctcaacga ttcgagagtt
      121 agctttttcc gcggatactt ttccgtggta aaagtcagtg agacttgcta aggtttgttc
      181 aaggaattct ttatctacaa aaatcatggt tgaaacttca gtagtaaact gggcatcaaa
      241 ttcttccaga ccttgctcag ctcgccaatt ggcaaactct tggtattggg tataggacat
      301 gctaatggaa atcccttcct gctccttaac ctccacaacc ccgatttcct tgactgcatt
      361 agccacgact ccagcgtaag cacgaataag tccgcctgcc ccgagtttga tacctccaaa
      421 atagcgggta accaccgttg cgacattggt cagttcgtga ttttctaaaa ccgtcagcat
      481 tggcacacca gcagtaccag acggctcccc atcatctgag gaacgcttga tttccatatt
      541 ttctccaagg acaaaagcag agcagttatg agtagccttg taatgttctt tcttgatttt
      601 gttgataaag tcgcgagctt cctcctcgct ggtcactcgt ttcataaagc agataaatcg
      661 tgacttttta atttcttcct caacaatacc atcttctcta attgttttaa attctttcat
      721 aaccaaattg t
//
LOCUS       DBNFEG010000288          726 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.290, whole genome shotgun sequence.
ACCESSION   DBNFEG010000288 DBNFEG010000000
VERSION     DBNFEG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.290"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(25..333)
                     /locus_tag="AC1PNG_002121"
     CDS             complement(25..333)
                     /locus_tag="AC1PNG_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702570.1"
                     /translation="MEFFGELLVEFLTGLADFDEKKHPPFGIRYWLGWLGVLIHVLLL
                     ALLTCVTVFFFKFFLVGKGLINVVVAVVFLLFALFWLWKSGKTILKMWQATIYYLAIH
                     "
     gene            complement(335..673)
                     /locus_tag="AC1PNG_002122"
     CDS             complement(335..673)
                     /locus_tag="AC1PNG_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP6702571.1"
                     /translation="MVAVPIWRSFLILLSFSSFFLVFYEDSQYKPFGFRYWLGLVACI
                     WLNFVTLASYFIAFTGGSIMVYNQYKQPLVVIFLFCLFVASMLTLLSFKAIKMMIRRT
                     KYYRQVRKVG"
BASE COUNT          287 a          163 c          119 g          157 t
ORIGIN      
        1 acaccagcag gtaaactggt gcctctaatg aatagctaaa taataaatcg ttgcttgcca
       61 cattttcaag attgtttttc cgcttttcca aagccaaaat agggcaaata gcaggaaaac
      121 aactgccact actacgttaa tcaagccttt tccaaccaag aaaaacttga aaaagaagac
      181 agtcacacaa gtgagcaagg caaggagtaa gacatgaatc agaactccca accagcccag
      241 ccaatagcga attccaaagg gagggtgttt cttttcatcg aagtctgcta gaccagtcaa
      301 aaactcaact agcaactcac caaaaaattc catactagcc tacctttctt acctgtcgat
      361 aatatttggt tcgtcgaatc atcatcttga tagccttaaa ggaaagtaag gttagcatac
      421 tagccacaaa tagacaaaaa aggaatatca caaccaatgg ctgtttatac tgattataga
      481 ccataattga accaccagta aaggcgataa aataggatgc caaagtcaca aaattgagcc
      541 agatacaagc cactagcccc agccaataac gaaatccgaa aggcttatac tgactgtctt
      601 cataaaatac aagaaaaaat gatgaaaaag aaagcaatat caaaaaagaa cgccaaatcg
      661 gaaccgctac cataccaaca cctccaagtc cattctagca tatttacgaa taggtgcaaa
      721 gaaaaa
//
LOCUS       DBNFEG010000289          722 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.291, whole genome shotgun sequence.
ACCESSION   DBNFEG010000289 DBNFEG010000000
VERSION     DBNFEG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.291"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..559)
                     /locus_tag="AC1PNG_002123"
     CDS             complement(<1..559)
                     /locus_tag="AC1PNG_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009526175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HIP6702572.1"
                     /translation="MNALLFDERPIVANPVLAREIGLNEAIVLQQINFWLEINKQNGK
                     NYHDGKYWTYNSIRAWHESDFSYLSYETVKRTFSKLEKAGYLFTGNYNKDPRDKTKWY
                     SLNYDKIFTLEEEVKQKRNNVASKVLEKADENDVTNALGQNDPMEKFKLIQCNNSERT
                     NGLVQTEPMQSVNMSQPLPNNTSNNT"
BASE COUNT          202 a          142 c          113 g          265 t
ORIGIN      
        1 ttgtattatt tgaagtatta tttggtaatg gttggctcat attgacactt tgcattggtt
       61 cagtttgaac caatccatta gttctttctg aattattgca ttggatcaat ttgaactttt
      121 ccattgggtc attttgaccc aatgcattgg taacatcatt ctcatctgcc ttttctagaa
      181 ctttacttgc tacgttattt ctcttttgct ttacctcctc ctctaatgtg aaaattttgt
      241 catagttcaa tgaataccat tttgttttat cccttggatc tttattgtaa ttccccgtaa
      301 ataaatagcc ggctttttct agttttgaaa atgtccgttt tacagtttca taacttaggt
      361 aagaaaaatc ggactcatgc caagcccgaa tagaattata agtccagtat tttccatcat
      421 ggtagttctt cccattctgt ttatttattt ctagccaaaa gttaatttgt tgaaggacga
      481 tagcctcgtt caatccaatt tctctggcca agacaggatt tgcgactatt ggtcgctcat
      541 caaacaacag tgcgttcata cacacctcct acgtcattct tcaaaaagaa aagcaaccag
      601 aattttctga ttgctcaata atctaaaata ttataaaacc ccaccacgtt taatcgtggt
      661 ggggtttgtg gtggggaatc aaatgattcc aaaccaattt aaatcaccat aaaggcgtta
      721 aa
//
LOCUS       DBNFEG010000290          716 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.292, whole genome shotgun sequence.
ACCESSION   DBNFEG010000290 DBNFEG010000000
VERSION     DBNFEG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.292"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            110..298
                     /gene="rpmB"
                     /locus_tag="AC1PNG_002124"
     CDS             110..298
                     /gene="rpmB"
                     /locus_tag="AC1PNG_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIP6702573.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
BASE COUNT          274 a          107 c          143 g          192 t
ORIGIN      
        1 aaataaaaat gaaaataatt cttgccaagt ccaatctttt ctgttagaat agtatggtat
       61 gtggtgctag cacatccgat atatttgatc aaatgaggag gaagatacaa tggctaaagt
      121 atgttatttc actggtcgta agactgtatc aggtaacaac cgttcacacg ctatgaacca
      181 aactaaacgc gcagttaaac caaaccttca aaaagtaact gttttgattg atggtaaacc
      241 taaaaaagtt tgggcttcag cacgtgcact aaaatcaggc aaagttgaac gcgtataata
      301 acaaagtcgt tagaaaaatc tagcgacttt tgtttttgga aaaaatagcc tagatataaa
      361 gtcggtgagg agcaaattcg gccactacat aataaacatg aaagaatatc agctatatag
      421 ctcttcacat tgctattgaa aagttaacaa agaaggatcg ttgtcaaaaa taaactgtgt
      481 attgatgtgg aagatttttc caagttcgaa catttaaact tggattgaca ttaggagttg
      541 tacgagagag catccgtacg ggttctacaa gaaaggaatc acgaactgga agaatagaga
      601 gtgtttaaaa aaatccaaaa aatctcaaaa aaccggttga caattgccaa agtagatgat
      661 agaataattg agttgtctct tgaagggcta gttaatagag gaacaaaaaa agtttc
//
LOCUS       DBNFEG010000291          709 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.293, whole genome shotgun sequence.
ACCESSION   DBNFEG010000291 DBNFEG010000000
VERSION     DBNFEG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.293"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            330..689
                     /locus_tag="AC1PNG_002125"
     CDS             330..689
                     /locus_tag="AC1PNG_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1252 family conjugative relaxosome accessory
                     protein"
                     /protein_id="HIP6702574.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVDNYRHFSPFVRDKILM
                     TDYKQLAAKEWFSLWQSQKFEQISRDVHEVLVVARENHQVTQEHVSILLTCVQELIAE
                     VSQVQPLSREFREKYMG"
BASE COUNT          217 a          120 c          163 g          209 t
ORIGIN      
        1 atagtttccg agagctaccc agctctcttt tttcttactc tcatttttca tactgtgata
       61 acttttccaa atcatccttg ttatcctaat atcattccta agaaaatcaa agtttgattt
      121 tctattgggg gtttgggggc gaagccacca agttatctta tcgttggctg tcaaaactgg
      181 aaggttttgg tcggctggcg ataagatttt tgggatattg tggacacaat atctgagctc
      241 gcaaagaccg aacaaggact ttaagaaatt tcttgtatga cacacggggt cggggtttac
      301 ccgagaaagg atgaaagtga gggaaagaga tgtctgaaca gtaccgtgac attagaaaag
      361 aggtaaatct aaccgcagat gagctaaaac agattgaaaa aatgatggag gttgataatt
      421 atcgtcactt ttcaccattc gtcagggata aaattttgat gactgattat aaacagttag
      481 ctgccaaaga atggttttcc ctttggcagt ctcaaaagtt tgaacagata agtcgagatg
      541 tgcatgaagt tctagttgtc gcaagagaaa atcaccaagt gactcaagaa cacgtgtcaa
      601 tcttattgac ctgcgttcaa gaattgattg cggaagtcag tcaagtacaa ccactcagtc
      661 gtgagtttcg tgaaaaatat atgggctagg agggtgtaat ggtttatcg
//
LOCUS       DBNFEG010000292          700 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.294, whole genome shotgun sequence.
ACCESSION   DBNFEG010000292 DBNFEG010000000
VERSION     DBNFEG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.294"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..534)
                     /locus_tag="AC1PNG_002126"
     CDS             complement(<1..534)
                     /locus_tag="AC1PNG_002126"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIP6702575.1"
                     /translation="MEKVSIIVPIFNTEKYLRECLDSIISQSYTNLEILLIDDGSSDS
                     STDICLEYAEQDGRIKLFRLPNGGVSNARNYGIKNSTANYIMFVDSDDIVDGNIVESL
                     YTCLKENDSDLSGGLLATFDGNYQESELQKCQIDLEEIKEVRDLGNENFPNHYMSGIF
                     NSPCCKLYKNIYINKGFD"
BASE COUNT          224 a          145 c           84 g          247 t
ORIGIN      
        1 gtcaaaacct ttgtttatat atatattctt ataaagtttg caacaagggc tattaaagat
       61 accgctcata taatgatttg gaaaattttc atttcctaag tctcgcacct cttttatctc
      121 ttccaaatca atttgacact tttgcagctc agattcttga taatttccat caaaagtagc
      181 aagtaaccct cccgacaaat cactatcatt ctcttttaaa caggtgtata aggactcaac
      241 aatgttgccg tcaacaatat catcagaatc tacaaacata atataatttg ctgtgctatt
      301 tttgataccg taattccttg cgtttgaaac accaccattt ggtaaccgga aaagttttat
      361 tctaccatct tgctctgcgt attccaaaca tatatccgtt gatgaatctg aagaaccgtc
      421 atctatcaaa agaatctcta gattagtata cgattgggaa ataatgctat ctaaacactc
      481 tcttaagtac ttttccgtat taaaaatagg tacaataatg ctgacttttt ccatcctcta
      541 attcctttta tgccgtaact tgctttgcta tcataaaaat catcatgtaa aaaatactat
      601 cttctatctt gccacctata gaacatatgc tctaccaaat taatagcaac tcaaaactct
      661 attcctgcca atttatgttt gcagttgttt gttaatagtt
//
LOCUS       DBNFEG010000293          690 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.295, whole genome shotgun sequence.
ACCESSION   DBNFEG010000293 DBNFEG010000000
VERSION     DBNFEG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.295"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            3..>690
                     /locus_tag="AC1PNG_002127"
     CDS             3..>690
                     /locus_tag="AC1PNG_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIP6702576.1"
                     /translation="MKSRIINDNLWVSTIHEFIWHIIGNFQKEIKECLVELINDDSQK
                     LFVSPKDVIEEESISLDYFDDVRVDYAEWYSMSITEQNKVQISHDHILIVAEKMFSKY
                     KKLCDVLADTANYIFVDEYQDTSPLIVKILLEHLQKSTKKNVVGFFGDSMQAIYDSGV
                     GDLNSYNLAKIQKTQNRRNPKKVIDLANKVRNDGLVQTPSNDDTAPNMDDGSVIAGTV
                     KFVYGNDINQL"
BASE COUNT          255 a           88 c          126 g          221 t
ORIGIN      
        1 aaattaaatc acgaataatt aatgataatt tatgggtatc aacaattcat gagtttatct
       61 ggcatatcat tggaaatttc caaaaagaga ttaaagaatg tcttgttgag ttaataaatg
      121 atgattcaca aaagttattt gtttcaccaa aggatgtaat tgaagaagaa agtatttcac
      181 ttgattactt tgatgatgtc cgtgttgact atgctgaatg gtactctatg agtataacag
      241 agcaaaataa agtccaaata agtcatgacc atattctaat agttgccgaa aaaatgtttt
      301 caaaatataa gaagttatgt gatgttttag cggatactgc aaactatatt tttgttgacg
      361 aatatcaaga tacaagtcca ctaattgtga aaatattatt agagcatctt caaaagagta
      421 ctaagaaaaa tgttgtaggc ttttttggag attctatgca agctatttat gattcagggg
      481 taggagactt aaattcttat aatttggcaa agatacaaaa aactcaaaat cgtcgtaatc
      541 ctaaaaaagt tattgattta gccaataaag ttagaaatga tggtttagtt caaactccat
      601 ccaacgatga tacggctccc aatatggacg atgggagtgt cattgctgga acagtgaaat
      661 ttgtctacgg gaacgatatt aatcaacttg
//
LOCUS       DBNFEG010000294          653 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.296, whole genome shotgun sequence.
ACCESSION   DBNFEG010000294 DBNFEG010000000
VERSION     DBNFEG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.296"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>653
                     /locus_tag="AC1PNG_002128"
     CDS             <1..>653
                     /locus_tag="AC1PNG_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GBS Bsp-like repeat-containing protein"
                     /protein_id="HIP6702577.1"
                     /translation="PTGTITIENRNDAQGTFDVRVTNVSSPKDIASVILPTWSQTDDL
                     RWYEATRQSDGSYKLTVNKKDHKYRTGTYTVHLYYKDSSGGLTGAGGTTTHLSEVKPT
                     GTITIENRNDAQGTFDVRVTNVSSPKDIASVLLPTWSQSDDIRWYEATRQSDGSYKLT
                     VNKKDHKYRTGTYTVHLYYKDSNGGLTGAGGTTTHLSEAKPTGTITIENRNDAQGTF"
BASE COUNT          208 a          129 c          139 g          177 t
ORIGIN      
        1 gccaacaggt acaatcacaa ttgaaaatcg taatgatgcc caaggtacct ttgatgttcg
       61 tgttactaat gtttcatcac cgaaagatat agcaagtgtt atcttaccca cttggtcaca
      121 gacggatgat ttacgttggt atgaagctac tcgtcagtca gatggcagct ataagttaac
      181 tgttaataag aaggaccata agtatcgtac aggtacctac acggttcact tatattataa
      241 ggactcaagt ggtggcctaa caggtgcagg tggtacaacg actcatttat cagaagtgaa
      301 gccgacaggt accatcacaa ttgaaaatcg taacgatgcc caaggtacct ttgatgttcg
      361 tgttactaat gtttcatcac cgaaagatat agcaagtgtt ctcttaccta cctggtccca
      421 gtcagacgat atacgttggt atgaagctac tcgtcagtca gatggcagct ataagttaac
      481 tgttaataag aaggaccata agtatcgtac aggtacctac acggttcact tatattataa
      541 ggattcaaat ggtggtttga caggtgcagg tggaacaaca actcatttat cagaagcgaa
      601 gccaacaggt acaatcacaa ttgaaaatcg taatgatgcc caaggtacct ttg
//
LOCUS       DBNFEG010000295          646 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.297, whole genome shotgun sequence.
ACCESSION   DBNFEG010000295 DBNFEG010000000
VERSION     DBNFEG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.297"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            83..>646
                     /locus_tag="AC1PNG_002129"
     CDS             83..>646
                     /locus_tag="AC1PNG_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
                     /protein_id="HIP6702578.1"
                     /translation="MWNPKKLTVLKWFDILILTIILFGDGIINSTLQYIALQNQTTTL
                     QENLTFSTMDNYKALALQLVWLTIAIFYLLLRNFDFSIWKKHIFITPWVPLQAVALFI
                     FSALCLDIYNLVSYQFLASNTPSMFQLFPNIDLSLILYSLLNGFYEEIFFLNLCLLVN
                     PKYAKWAFLYSLIIRCSFHTYQGLISAL"
BASE COUNT          208 a          120 c           81 g          237 t
ORIGIN      
        1 ataaaaaaca gacgaaaccg agagcatata tagatttgtt tgataaactg aactaaacaa
       61 aaatgaaagg aactctccca ttatgtggaa tccaaaaaaa ttaaccgtat taaaatggtt
      121 tgatattctc attctaacaa ttattttatt tggagatgga atcatcaact ctactctcca
      181 atacatagcc ttacaaaatc aaactacaac tcttcaagaa aatttgacat tttcaacaat
      241 ggataattat aaggctttgg ctctacagtt agtttggctc acaatagcca tcttctatct
      301 attactccga aattttgatt tctcaatttg gaaaaaacat atttttatta ctccttgggt
      361 tccattacaa gctgttgcct tattcatttt ttctgcatta tgcttggata tctataatct
      421 agtatcctat cagtttctag cttccaacac tccatccatg tttcaactat ttcctaacat
      481 tgatttatca ctgatcttgt actcattgtt gaacggattt tatgaagaaa ttttcttcct
      541 taacttgtgc ttactggtta accctaaata tgcaaaatgg gcatttttat attcgttaat
      601 tattcgatgt agtttccata cctatcaagg tttaattagt gcactc
//
LOCUS       DBNFEG010000296          643 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.298, whole genome shotgun sequence.
ACCESSION   DBNFEG010000296 DBNFEG010000000
VERSION     DBNFEG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.298"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
BASE COUNT          204 a           73 c          111 g          255 t
ORIGIN      
        1 attgagtatt atctgtttag ttctttctct ctttcatttt gtgtttataa tgttttaggt
       61 gattgtggga cgttttcagt gaaactgcta atggaacggt tttactccta gccttatctg
      121 agtaataata ggataaaact catataccca cataagagtg atagaggttt gtgaatagta
      181 tttagttaga aacatttgtt atcaaataca ggttctacgt tatatttaaa gaacttctct
      241 atcattcgtt aaatatttct tctccgattt atcgctagat aggagcagtg tatgcgtttc
      301 taaaaatcta aagttggagg atgttcaatt ttaaataagt tacaatctgc aaattactac
      361 taaaatcaat ttatccctta tacagggtaa tgtaataaat tgttttttct ttgtttttcg
      421 tatgtttttt caaaaaaata tatgaaacaa atgaggggaa tctagtgatg cactagctat
      481 gaaaaaaata tttatattaa tggaattgtt tgagaaatca gaaggatgta gtttttcgat
      541 ttgcatacat attttaactt aaacatatgt gtgcaaaaag ttctgatttt tttgtgtttt
      601 ttaagaaatt ggcattgttg acaaactaag gattggtttg gta
//
LOCUS       DBNFEG010000297          637 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.299, whole genome shotgun sequence.
ACCESSION   DBNFEG010000297 DBNFEG010000000
VERSION     DBNFEG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.299"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..624)
                     /locus_tag="AC1PNG_002130"
     CDS             complement(<1..624)
                     /locus_tag="AC1PNG_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIP6702579.1"
                     /translation="MISNEQYDYLRAHPTFERLTRDSFDQLAKHIRFRKIPKGQIFFY
                     AEDPRDYLFVLYKGYARIEQYDETDSFTYLDYIRQGGAFPFGDMFQDKPYHYTAIAIT
                     DLEYFIVPMVLFETLSKINPSQMVYICQKLSKVLRFQELRLRNAMRSKASDRVVQALA
                     LLYWDMCQRDQFATLPFEIHIQEISRLAATTRETVSHVLKQLKQDGKI"
BASE COUNT          205 a          143 c          112 g          177 t
ORIGIN      
        1 aattttacca tcctgcttca gttgcttcaa aacatggcta accgtttccc gtgtcgtagc
       61 tgctaaacgc gaaatctctt gaatatgaat ttcaaatggc aaggtagcaa actgatcgcg
      121 ctggcacata tcccaataaa gaagagccaa ggcttgaacg actcgatctg atgccttgga
      181 acgcatcgca tttcgcaagc gcaactcttg aaaacgaagc actttggata atttttgaca
      241 gatataaacc atttgactcg ggtttatctt cgacaaggtt tcaaacaaga ccatcggtac
      301 aataaaatac tctaggtccg ttatggcaat ggcggtataa tgataaggtt tgtcctgaaa
      361 catatctcca aaaggaaagg caccaccctg ccgaatatag tccaaatacg taaaactatc
      421 cgtctcatca tattgctcaa tccgagcata acccttatac aatacaaata gataatctct
      481 aggatcctct gcgtaaaaaa agatttgccc cttaggaatc tttcgaaaac gaatatgctt
      541 agctaattga tcaaagctat cacgtgtcaa acgttcaaaa gttggatgtg cccttagata
      601 atcatattgt tcgttgctta tcatcaaaac tcctttc
//
LOCUS       DBNFEG010000298          631 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.300, whole genome shotgun sequence.
ACCESSION   DBNFEG010000298 DBNFEG010000000
VERSION     DBNFEG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.300"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            29..466
                     /locus_tag="AC1PNG_002131"
     CDS             29..466
                     /locus_tag="AC1PNG_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIP6702580.1"
                     /translation="MSYSFQNPSQDIIFDYLKNAKTIAVVGLSSREETAAYRVSKLMQ
                     EAGYKIIPVNPKAAGGTILGELVYSSLAEIDQPIDIVDVFRRSEFLPEVAQEFIQSNA
                     KIFWAQLGLESQEAEKLLRQAGRNDIVMNKCIKIEYLEMKEQY"
BASE COUNT          210 a          116 c          134 g          171 t
ORIGIN      
        1 catttaatat acattaaagg aggtctctat gtcttattcc ttccaaaatc ctagtcagga
       61 cattatcttt gactatctga aaaatgctaa gaccattgca gtggtcggtt tgtctagtcg
      121 agaagagacg gcagcctacc gcgtttccaa gctcatgcag gaagcgggct ataaaattat
      181 tccagttaat cccaaggctg ctggtgggac tattttaggc gagctggtct atagtagtct
      241 agcagagatt gaccaaccta ttgatattgt agatgttttc cgcagaagtg aatttttacc
      301 agaggtagca caggaattta tccagtcgaa cgccaaaatt ttctgggctc aactcggttt
      361 agaaagccaa gaagcagaaa aactattgcg tcaggcaggt cgtaacgata ttgtcatgaa
      421 taaatgtatc aagattgaat atcttgaaat gaaagaacaa tactaataag tcgggtatat
      481 ttatccgact tttttcatcg aaaaagcagt tcaatgaacc attgaactgc cggatgactc
      541 taaggaggag cacttagaat tggaacaaat tactacacat ggcgtaaaaa atagaaagga
      601 agaaatctgc tccaatacca agcaggctaa a
//
LOCUS       DBNFEG010000299          608 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.301, whole genome shotgun sequence.
ACCESSION   DBNFEG010000299 DBNFEG010000000
VERSION     DBNFEG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.301"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>608
                     /locus_tag="AC1PNG_002132"
     CDS             <1..>608
                     /locus_tag="AC1PNG_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702581.1"
                     /translation="NGIDKAERILIDYFKNEVRKKVHWIKKSSKVLLDRSHLIDVFFE
                     EHFKGNYICSVPLGLIIVDGAVNDFTKNKDNKGLFAEGLELNVWDSFVGSNKSLKEIV
                     SIFTSSRRKTNKDEIRLPYRNGILHGRDINFGNEYVSCKCVSLLFAVADWIKQKTNEV
                     ERKDSFVKSQTNESIVDLINRYQELKKFEDEIANWKRRDIIL"
BASE COUNT          237 a           55 c          124 g          192 t
ORIGIN      
        1 aaaatggtat tgataaagca gaaagaatac tgatagatta cttcaaaaat gaagtaagaa
       61 aaaaagtgca ttggattaaa aaaagttcca aagttctttt agataggtct catttgatag
      121 atgtattttt tgaggaacat tttaaaggca attatatttg tagcgtgcca cttggtttaa
      181 tcattgttga cggtgcagtt aatgatttta caaaaaataa agataacaaa ggtttgtttg
      241 cagaaggttt agaactaaat gtatgggatt catttgtggg tagtaataag tcgttaaaag
      301 aaatagtatc aatttttaca tctagtcgac gaaaaacgaa taaagatgaa attagattac
      361 catacagaaa tggtatatta catggaaggg atataaattt tggtaatgag tatgtatctt
      421 gcaagtgtgt ttcattactt tttgctgtcg ctgattggat aaagcaaaaa acaaatgagg
      481 ttgagagaaa agatagtttt gttaaatcac aaacaaacga atctattgtg gatttgatta
      541 atagatatca ggaactaaag aagtttgagg atgaaatagc aaattggaaa agacgggata
      601 taattctc
//
LOCUS       DBNFEG010000300          607 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.302, whole genome shotgun sequence.
ACCESSION   DBNFEG010000300 DBNFEG010000000
VERSION     DBNFEG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.302"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>607
                     /locus_tag="AC1PNG_002133"
     CDS             <1..>607
                     /locus_tag="AC1PNG_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6702582.1"
                     /translation="SKKFKNCDLFDNLHFECSEGEIVGITGINGSGKSVLFKLIAGLM
                     QPNSGTIKVYGEDIVGKIPSGLGALIEEPGFIPNYSGFENLEFLSTIQNIIGKEEILA
                     TLHRVGLFEDKDKKVRNYSLGMKKKLGIAQAIMEEPRILLLDEPTNALDSQSVQRVYD
                     LLKELSKEKGVTVLLASHNDSDIRHLCDKVYLLENKQCKLLK"
BASE COUNT          207 a           74 c          139 g          187 t
ORIGIN      
        1 agcaaaaaat ttaagaattg tgatttattt gataatttac actttgagtg ttcggaaggg
       61 gaaatagtag ggataactgg tataaatggt tcggggaaga gtgttctgtt caaattgata
      121 gcgggcctta tgcaaccgaa ttctgggact atcaaggtgt atggagaaga tattgtcgga
      181 aagattccat ctggtttagg agcattaatt gaagaaccag gctttattcc caattatagt
      241 ggatttgaaa atttagaatt tttatcaact atccaaaata tcattggtaa ggaagaaata
      301 cttgcaacct tacatcgtgt aggactcttt gaagataagg ataaaaaggt taggaattac
      361 tctctcggta tgaagaaaaa attaggaatt gcacaggcta ttatggaaga gcctagaatc
      421 cttctattgg atgagccgac aaatgctttg gatagtcaga gcgttcagag ggtgtatgac
      481 ttgctaaaag aactttcaaa agaaaagggt gttacggttt tattggccag tcataatgat
      541 agtgatatta gacatctatg tgataaagtt tatcttctcg aaaataagca atgtaagtta
      601 ttgaaat
//
LOCUS       DBNFEG010000301          607 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.303, whole genome shotgun sequence.
ACCESSION   DBNFEG010000301 DBNFEG010000000
VERSION     DBNFEG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.303"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            1..607
                     /locus_tag="AC1PNG_002134"
                     /pseudo
     CDS             1..607
                     /locus_tag="AC1PNG_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935295.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          183 a          141 c          123 g          160 t
ORIGIN      
        1 tttgggattg atgtgagtaa agtaagttca gaagtagcca ttctagtcaa cggcgagaag
       61 gttcataact acaccatgtc caatgatgcc attggctttt ctcggctact tggcgatttg
      121 agaaccgtcc ataagccaga aatcatcttt gaagcaacag gcgtctattc tcgccgtctc
      181 caaacttttc tagaagataa tggctatgct tatacacggc ttaatccctt agaagctaag
      241 aagcaactgg atagcttgcg tgtgcggaaa acagatcaaa ttgacgccga aaaactggct
      301 caatctcaat ttgtgctgaa tcgtaaaccc acttatgtcc aagaagaagt ctaccaaaac
      361 ttgcgggatc tcagccgttt ctatcagaat ctgaccgagg acattgttcg agccaaaaac
      421 cgtctgcaca aggtcttaca agtcactttt cctgaattgg aaaatatctt atcaacacca
      481 tctggcgaac aatactgaaa cctggtcata gcttttcctt gcaaggactt cgtgcttgat
      541 ttaagcaagg acgaactctc agaaagcatc cgtcagtcca cttcaaaacg catatctgac
      601 aagcgtg
//
LOCUS       DBNFEG010000302          585 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.304, whole genome shotgun sequence.
ACCESSION   DBNFEG010000302 DBNFEG010000000
VERSION     DBNFEG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.304"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..>585)
                     /locus_tag="AC1PNG_002135"
     CDS             complement(<1..>585)
                     /locus_tag="AC1PNG_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase Wzy"
                     /protein_id="HIP6702583.1"
                     /translation="GATMFTGAVEGIGFSQGFNGGLTHKNFFGITILMGFVLTYLAYK
                     YGSYKRTDRFILGLELFLILISNTRSVYLILLLFLFLVNLDKIKIEQRQWSTLKYISM
                     LFCAIFLYYFFGFLITHSDSYAHRVNGLINFFEYYRNDWFHLMFGAADLAYGDLTLDY
                     AIRVRRVLGWNGTLEMPLLSIMLKNGFIGLVGYGI"
BASE COUNT          229 a          124 c           72 g          160 t
ORIGIN      
        1 aatcccatac cctaccagac cgataaaacc attttttaac atgatactca gtaagggcat
       61 ttcaagcgtt ccattccaac ctaaaacgcg tctaaccctt atagcatagt ctaaagttaa
      121 atccccatat gccaaatccg ctgcaccaaa cattagatgg aaccaatcat ttctataata
      181 ctcaaaaaaa ttaataagac cattaacgcg atgagcgtaa gaatcactat gtgttattaa
      241 aaaaccaaag aaatagtata aaaaaatagc acaaaatagc atggaaatat atttaagcgt
      301 actccattgt ctttgttcta ttttgatttt gtcaagatta acaagaaata gaaaaagcaa
      361 tagtattaaa taaactgagc gtgtgtttga aataagaatc aaaaacaatt ctaatcctaa
      421 aataaaacga tccgttcttt tataggaacc atacttatac gccaagtaag ttaatacgaa
      481 ccccattaaa atagttattc caaaaaagtt cttatgcgtc aatcctccat taaaaccctg
      541 actaaaaccg ataccttcta ctgcccccgt gaacatcgtt gcccc
//
LOCUS       DBNFEG010000303          584 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.305, whole genome shotgun sequence.
ACCESSION   DBNFEG010000303 DBNFEG010000000
VERSION     DBNFEG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.305"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            48..221
                     /locus_tag="AC1PNG_002136"
     CDS             48..221
                     /locus_tag="AC1PNG_002136"
                     /inference="COORDINATES: protein motif:HMM:NF025153.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HIP6702584.1"
                     /translation="MPQGLVTPEETYFFNQENIVAIVFRGYVNIDEQSFSSQCDTLIS
                     ESGYEKGKVLSYN"
     gene            297..>584
                     /locus_tag="AC1PNG_002137"
     CDS             297..>584
                     /locus_tag="AC1PNG_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009853642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702585.1"
                     /translation="MSKHKKTVWNASLEEGTGLVTDRYIQTSMEDIEKNGYGKKIIGF
                     LTVNLFMYLITFIGRYVDSQIILTLFKSTEVLVSIPFFLGLLVTLLYFISLK"
BASE COUNT          193 a           70 c          117 g          204 t
ORIGIN      
        1 gttcttcagg atggaaaaga agtttatttt gattatgggc agttcgaatc ccacagggat
       61 tggttactcc agaggaaact tactttttca atcaagaaaa cattgtagct atagtatttc
      121 gtggttatgt caacattgat gagcaatctt tttcaagtca gtgtgataca ttgatttcag
      181 aatctggtta tgaaaaagga aaagttctat catataatta gtaatttgaa tcctcagtaa
      241 attgtttgct ggggattatt tattgtataa taggaaagta tgataatctg ataaggatga
      301 gtaaacataa aaaaacagta tggaatgcct ctttagaaga gggcacgggg ttggtaacgg
      361 atcggtacat tcagacttca atggaggaca tagagaaaaa tggttatgga aagaaaataa
      421 taggtttttt gactgttaat ctcttcatgt atttaataac atttataggt agatatgttg
      481 attcacaaat catattaact ctatttaaaa gtacggaggt attagtaagt attcctttct
      541 ttctagggct acttgttaca cttttgtatt tcatttcctt gaaa
//
LOCUS       DBNFEG010000304          581 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.306, whole genome shotgun sequence.
ACCESSION   DBNFEG010000304 DBNFEG010000000
VERSION     DBNFEG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.306"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..214)
                     /locus_tag="AC1PNG_002138"
     CDS             complement(<1..214)
                     /locus_tag="AC1PNG_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HIP6702586.1"
                     /translation="MKFSKRHYRAQVDSKDCGVAALAMVLEYYNSHYSLAELREMLGT
                     TENGTTAFSIVSVANELGFDTEAIYAD"
BASE COUNT          212 a          119 c           70 g          180 t
ORIGIN      
        1 tatctgcata aatagcttca gtatcgaacc ctaattcatt tgccacagaa actatactaa
       61 atgcagtagt cccattttca gttgtaccta acatttctct aagttctgct aaagaataat
      121 gtgaattata gtattctaac accatggcca aagcagctac tccacaatct tttgaatcaa
      181 cttgagcacg ataatgacgt ttcgaaaatt tcattcactc aatatactcc ttactagtat
      241 tagtgtttag ccttgatata taataaaatg actaatgaag cataataaag ttatatgcta
      301 tctataatta aaatattgga gcaacaacta gggatcaaac acaaaaatgg gtgagatttt
      361 cacccataaa aatcatatag caaaacttac tcaaaaaaaa ctcccctata ttattatcaa
      421 tttttcttac atacacgcct catataggca caagaacttc ctggatcaga agtacctaag
      481 ccagctaaaa ttatatcatg agcacaagtt acaatcgccc atttacaatt agcactttcc
      541 gatgcacctc ctcttatttc attaagaact gttttagaaa t
//
LOCUS       DBNFEG010000305          574 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.307, whole genome shotgun sequence.
ACCESSION   DBNFEG010000305 DBNFEG010000000
VERSION     DBNFEG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.307"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..289)
                     /locus_tag="AC1PNG_002139"
     CDS             complement(<1..289)
                     /locus_tag="AC1PNG_002139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HIP6702587.1"
                     /translation="MLLLALSYIFLSKYEFQKSACIFLICLYRGSDALSDVFQGLFQQ
                     NARLDIAGKSLFLRNSIVILTFGFGLFITNNLLLSLIYLVISSYLFVFFFDV"
     gene            complement(301..>574)
                     /locus_tag="AC1PNG_002140"
     CDS             complement(301..>574)
                     /locus_tag="AC1PNG_002140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HIP6702588.1"
                     /translation="QGEDSVKSRIVSPKTIFIWNLLGSISSAAISIFLLLLVTRLLTE
                     LEADIFSFAYAVANLFVIIASFQVRDYQATDVSKKFSLASILQQGL"
BASE COUNT          230 a           97 c           79 g          168 t
ORIGIN      
        1 taacatcaaa aaagaaaaca aataaatatg agctaattac tagatagatt aatgagagta
       61 gcaggttatt ggttataaac aaaccaaatc caaatgtcaa aatcacaata gagtttctca
      121 aaaaaagtga tttacctgca atatctagcc gagcattttg ttggaacaat ccttgaaaaa
      181 catccgatag agcatcactt ccacgataca aacatattag gaaaatacaa gcagattttt
      241 gaaattcata tttagacaga aaaatatagc ttaaggcaag taatagcata atagtgattg
      301 ttataagcct tgttgcaaga tactggctaa cgaaaacttt ttcgaaacat cagtagcttg
      361 ataatctctt acctgaaaac tagcaatgat tacaaaaaga tttgctactg cataagcaaa
      421 actaaaaata tcagcctcta attccgtcaa caaacgtgtt actagtaaca ataaaaatat
      481 tgatattgct gctgaagaaa tacttcctaa taaattccaa ataaatattg tttttggtga
      541 tactattctg ctcttcacac tatcttcccc ttgt
//
LOCUS       DBNFEG010000306          567 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.308, whole genome shotgun sequence.
ACCESSION   DBNFEG010000306 DBNFEG010000000
VERSION     DBNFEG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.308"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(120..207)
                     /locus_tag="AC1PNG_002141"
     tRNA            complement(120..207)
                     /locus_tag="AC1PNG_002141"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(171..173),aa:Ser,seq:gga)
     gene            complement(210..557)
                     /gene="ssrA"
                     /locus_tag="AC1PNG_002142"
     tmRNA           complement(210..557)
                     /gene="ssrA"
                     /locus_tag="AC1PNG_002142"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          169 a          135 c          100 g          163 t
ORIGIN      
        1 tatcatattt ttgaagaaaa acaagaattt taccataaaa cctgaacttt cacttgtttt
       61 tcagttgtat cattcacaga caaaaaaaga acgactatca gtcgttcttc aataacatac
      121 ggaagacatg ggattcgaac ccacgcacgc tgttacacgc ctaccgcgtt tccaacacgg
      181 cctcttgagc ctcttgagta atcttccata tatggagctg gtgggagtcg aacccacgtc
      241 caaacacctg ctaacttatt cgtctacaac cataggttat gtcttgcttt aacttgaact
      301 cgatacataa ctcaaaccta gaccaagcga gtcagtaaat ctcttttgga actgcctgac
      361 tcaatcccaa cgtagcttgc tattttaaga ccaatcagca aacacaagca attcgcgact
      421 ggtcacgcag gcaggttatt aagctgctaa tgcgtaagtg tttgtatttt ttgcagttat
      481 atttaactgg catttttaca tctgccgatg agttgcagaa caagcctcat aatgcctgtc
      541 gaatccgtaa caaccccgta ggatttg
//
LOCUS       DBNFEG010000307          563 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.309, whole genome shotgun sequence.
ACCESSION   DBNFEG010000307 DBNFEG010000000
VERSION     DBNFEG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.309"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..537)
                     /locus_tag="AC1PNG_002143"
     CDS             complement(<1..537)
                     /locus_tag="AC1PNG_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIP6702589.1"
                     /translation="MKLIKQLIHILISIGLIGSFLLYTHLIQNELGSTKNDGTVEIIA
                     EQPNQVIDSIQINGRYIHSSEIIENQWGINDADLIPNFSSLGEENSLVIKSSSSVRTL
                     SFEYFVSENPTIVKIKVGGQLVESIDTSTGDKYKNLAFIELPYTLRITKDNQFWYLHL
                     IVLALGLTCFILNGATWRI"
BASE COUNT          179 a           82 c           95 g          207 t
ORIGIN      
        1 gattctccat gttgctccat ttaagataaa gcatgttaga cctaaagcaa ggactattaa
       61 gtggaggtac caaaattgat tatctttagt tatccgtaaa gtatagggaa gctcaataaa
      121 ggctaaattt ttatatttat ctccagttga tgtgtcaatg gattcaacca attgtccacc
      181 gactttaatt tttacaatag tcggattttc actaacaaag tattcaaatg ataaggttct
      241 tacagaagag gaagatttaa ttacgagtga attttcctca cctagagaag agaaattagg
      301 gattaaatca gcatcattga ttccccattg gttttctata atttctgatg aatgaatata
      361 gcgtccattt atttggatgc tgtcgattac ttggtttggt tgttctgcta taatttctac
      421 agttccatca tttttcgtag aacctaattc gttttgtatt agatgtgtat acagtaaaaa
      481 acttccaatc aatccgatgg agattagtat gtgaataagc tgtttgatta atttcatatt
      541 tattaacaat tctaagtttt ata
//
LOCUS       DBNFEG010000308          518 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.310, whole genome shotgun sequence.
ACCESSION   DBNFEG010000308 DBNFEG010000000
VERSION     DBNFEG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.310"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>518
                     /locus_tag="AC1PNG_002144"
     CDS             <1..>518
                     /locus_tag="AC1PNG_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISSsu6 family transposase"
                     /protein_id="HIP6702590.1"
                     /translation="AMTHIAHRLSISTSSVMRKLNEFKFETDWNTLPEVMSWDEYSFK
                     KGKMSFIAQDFNSLNVITILDGRTQATIRNHFLRYPRKVRNQVKVITMDMFSPYYQLA
                     KQLFPNAKIVLDRFHIVQHLSRAMNRIRIQIMNQFDRTSQEYRALKRYWKLIQQDSRK
                     LSDKRFYRPMFR"
BASE COUNT          172 a          108 c           92 g          146 t
ORIGIN      
        1 gcaatgacac atattgcaca caggctatct atctcaacct catcagttat gagaaagctt
       61 aatgagttca agtttgaaac agattggaat accttacctg aggtgatgag ctgggatgaa
      121 tatagcttca agaaggggaa aatgagcttt atcgctcaag atttcaactc cctaaatgtc
      181 attactattc tggacggaag aacacaagca accatccgaa accactttct acgctatcct
      241 agaaaggtta gaaatcaagt caaagtcatt accatggata tgtttagtcc ctattatcaa
      301 cttgctaaac agctttttcc gaatgcaaaa attgtactcg accgctttca tattgttcaa
      361 caccttagtc gtgctatgaa ccgtattcgt attcaaatta tgaatcaatt cgacagaaca
      421 tcgcaggaat acagagcctt gaaacgctac tggaaattga ttcaacagga tagtcgtaaa
      481 ctcagcgata aacgatttta tcgccctatg tttcgaat
//
LOCUS       DBNFEG010000309          515 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.311, whole genome shotgun sequence.
ACCESSION   DBNFEG010000309 DBNFEG010000000
VERSION     DBNFEG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.311"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..>515)
                     /locus_tag="AC1PNG_002145"
     CDS             complement(<1..>515)
                     /locus_tag="AC1PNG_002145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIP6702591.1"
                     /translation="SVIIPAYNVADYLEECVYSVLNQTYEEFEILLVDDGSTDGITSN
                     ICDKLAAKDDRVKVFHQTNGGQSIARNTGIKNASGDYILFLDGDDFWTDVHFLDEINE
                     ELHSHEEVVDAVIYPFSYWYGNADIRVRDFPQKLPRHEIITDSVLLVETGALIAPVWN
                     KCVRRELFADS"
BASE COUNT          172 a          111 c           71 g          161 t
ORIGIN      
        1 agaatctgca aatagttctc tgcggacaca cttgttccaa acaggagcaa tcaatgcgcc
       61 tgtttctaca agtagaacag aatcagtaat aatttcatgt ctaggtaatt tttgagggaa
      121 atcacgaact cttatatcag cattaccata ccagtatgaa aatggataaa ttactgcatc
      181 taccacttcc tcgtgagagt gtagttcttc attgatttca tctaaaaaat gcacatcagt
      241 ccaaaaatca tcaccatcta gaaataaaat ataatcacca ctagcatttt ttattcctgt
      301 atttctcgca attgattgcc caccattagt ttgatgaaat accttcaccc tatcatcttt
      361 agctgctaat ttatcgcata tattggaggt tataccatct gtagaaccgt catctaccaa
      421 caatatttca aattcttcat aagtctgatt cagtacacta tacacacatt cttccagata
      481 atcagcaaca ttgtacgctg ggataataac ggaaa
//
LOCUS       DBNFEG010000310          505 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.312, whole genome shotgun sequence.
ACCESSION   DBNFEG010000310 DBNFEG010000000
VERSION     DBNFEG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.312"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..392
                     /locus_tag="AC1PNG_002146"
     CDS             <1..392
                     /locus_tag="AC1PNG_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702592.1"
                     /translation="DEKIKTVNHDQIKQYYLENNLNFARQDLINGKISTWNDGLIIST
                     ESVEINEETVRLVTERFPDLELQLENIEVGQQFSWKQNGIYYTFSCLSREDKGIIPLS
                     EITDAVAMQCAEELVDNWLNKETDSLK"
BASE COUNT          189 a           60 c           86 g          170 t
ORIGIN      
        1 ttgatgaaaa aataaaaacc gtaaatcatg atcagattaa acaatactat ttagaaaata
       61 atctaaattt tgctcgtcaa gacctaataa atggaaaaat ctctacttgg aatgatggat
      121 taattatttc tacagaatct gttgaaataa atgaagaaac tgttagattg gtcactgaga
      181 gatttcctga tttagaattg caattagaga atatcgaggt gggccaacag ttttcatgga
      241 agcaaaatgg tatttattat actttctctt gtctttctag ggaagataag ggaattattc
      301 cactttctga aattactgat gcagttgcta tgcaatgtgc cgaggaactt gttgataatt
      361 ggttaaataa agaaactgac tctttaaaat agtccataat tattgacaat ctaaagttaa
      421 caaaaatttt atgttaattt caaaggaatt gggaatattc gtactgaaaa actgatttca
      481 attataatgt aagtgtctta gttaa
//
LOCUS       DBNFEG010000311          460 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.313, whole genome shotgun sequence.
ACCESSION   DBNFEG010000311 DBNFEG010000000
VERSION     DBNFEG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.313"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(110..>460)
                     /locus_tag="AC1PNG_002147"
     CDS             complement(110..>460)
                     /locus_tag="AC1PNG_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001540 - amyloid-beta binding [Evidence
                     IEA];
                     GO_function: GO:0004930 - G protein-coupled receptor
                     activity [Evidence IEA];
                     GO_process: GO:0097190 - apoptotic signaling pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HIP6702593.1"
                     /translation="LQKELEDLDDEALNVLMMVEMRQPLVALILAIFFGEFGVDRFYV
                     GNKELGFAKLIAFAVSFVTLFILIGFLLFLGLYLWKFIDCFLIMRACKEANFERLMLQ
                     IHQYKAFQHSNQTF"
BASE COUNT          175 a          109 c           63 g          113 t
ORIGIN      
        1 tgtcacttct gtgacaaagc tcgttccata ttacaagtac tttacagcct actaaaaaca
       61 gaaaaggaag acactaccaa gcctcccctt attcccaaaa atatcttact taaaatgtct
      121 gattgctatg ctgaaaagcc ttatattgat ggatttgtag catcaaacgc tcaaagttcg
      181 cttccttaca tgctcgcatg atcaagaagc aatcaataaa cttccacaaa taaagtccca
      241 aaaataacaa gaacccaatc aagataaata gcgtaacaaa agatactgca aaagcaatca
      301 gcttggcaaa tcccaattct ttattcccga cataaaaacg gtcgacacca aactcaccga
      361 agaaaatagc caaaataaga gcgacaagag gttgacgcat ctcaaccatc ataagaacgt
      421 ttaaagcctc atcatctaaa tcttctagtt ctttttgcag
//
LOCUS       DBNFEG010000312          449 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.314, whole genome shotgun sequence.
ACCESSION   DBNFEG010000312 DBNFEG010000000
VERSION     DBNFEG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.314"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            139..>449
                     /locus_tag="AC1PNG_002148"
     CDS             139..>449
                     /locus_tag="AC1PNG_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702594.1"
                     /translation="MRKKLIATIAFCVVCLFLYSFVPQSFGTPTIFQTPYKTSVKSEF
                     EALFHKHGIDVSENDFWATSLNGRTPQDILNEKAKLLYIKFNLLEKEARKYGFNKEKL
                     D"
BASE COUNT          178 a           56 c           60 g          155 t
ORIGIN      
        1 taagaatttt taaagtgaaa atcaaaatta cagtctgatt ctcaataaat tgtaatataa
       61 atttacataa taaatatttc agatatgata ttaataataa ttgagataaa taaattattt
      121 ggataaggag gtaacaatat gagaaaaaaa ttgatagcca cgattgcatt ttgcgtagtt
      181 tgtctatttc tctattcatt tgttcctcaa tctttcggta ctccaactat ttttcagaca
      241 ccttataaaa cctctgtgaa atccgaattt gaagcattat ttcataaaca tggtattgat
      301 gtctcagaaa atgatttttg ggctacatca ttaaatggtc gaacacctca agacattcta
      361 aatgaaaaag caaaattact atatataaaa ttcaatttat tagaaaaaga agcccggaaa
      421 tatggtttta ataaagaaaa gttagattt
//
LOCUS       DBNFEG010000313          447 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.315, whole genome shotgun sequence.
ACCESSION   DBNFEG010000313 DBNFEG010000000
VERSION     DBNFEG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.315"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..376
                     /locus_tag="AC1PNG_002149"
     CDS             <1..376
                     /locus_tag="AC1PNG_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIP6702595.1"
                     /translation="LEYCSAKRIPIGIITNGPVKHQLKKLSQLDVLKYFDKQKIIISQ
                     ATGFQKPQIEIFNLASKNFNFLPNQTLYISDNFKNDIEGSLNAGWKSIWFNHRKRKLP
                     IDTKIHEVQTAKDLKELVYRLL"
BASE COUNT          167 a           76 c           60 g          144 t
ORIGIN      
        1 tcttgaatac tgttcggcaa aaaggattcc cattggaatc ataacgaatg gtccagttaa
       61 acatcaatta aaaaaattat cacaattaga cgttctaaaa tactttgata aacagaaaat
      121 tatcattagc caagcaactg gtttccaaaa acctcaaatt gaaattttta atctcgcttc
      181 taagaatttc aattttctcc ccaaccaaac actttatatt agtgataact ttaaaaatga
      241 tattgaagga agcttaaatg ctggttggaa atccatttgg tttaatcaca ggaaacgcaa
      301 actacctatt gatactaaaa ttcatgaagt tcaaactgct aaagacttaa aagaactagt
      361 ctaccgactg ctttaagttc aatataatat tttttgacca gtcaatagac tggttttgtt
      421 tattctaggg aaatatctcc ttgaaat
//
LOCUS       DBNFEG010000314          442 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.316, whole genome shotgun sequence.
ACCESSION   DBNFEG010000314 DBNFEG010000000
VERSION     DBNFEG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.316"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..>442)
                     /locus_tag="AC1PNG_002150"
     CDS             complement(<1..>442)
                     /locus_tag="AC1PNG_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HIP6702596.1"
                     /translation="IYSDIVFVSNSHVSKDIVQYLQSERLIDFFIQRDNIGYDFAAWK
                     EGLNQVTFYQYDSVTLMNDTCFGPLWDLEDYYSQFDSDVDVDFWGMTNHLETKIDSVV
                     VPEHLQSYFMVFKKQILQSQAFVGFWSSVSELTDIQDVIKLYESQ"
BASE COUNT          152 a           91 c           57 g          142 t
ORIGIN      
        1 attggctctc atataatttt ataacatctt gaatatctgt taactcactt accgatgacc
       61 aaaaaccaac aaatgcttga ctctgcaaga tttgtttttt aaataccata aaatatgact
      121 gtaaatgttc cgggacaact acactatcta tttttgtctc taagtgattg gtcatccccc
      181 aaaaatctac atcaacatca ctgtcaaatt gactataata atcctctagg tcccagaggg
      241 ggccgaaaca tgtatcattc atcagagtta ccgaatcata ttgataaaat gttacttgat
      301 tcagcccttc tttccatgcc gcaaaatcat aaccaatatt atctctttgg ataaaaaaat
      361 caatcaatcg ttccgactgt aaatattgta ctatgtcctt tgaaacatgg ctgttagaaa
      421 caaaaactat gtctgaataa at
//
LOCUS       DBNFEG010000315          442 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.317, whole genome shotgun sequence.
ACCESSION   DBNFEG010000315 DBNFEG010000000
VERSION     DBNFEG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.317"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..333
                     /locus_tag="AC1PNG_002151"
     CDS             <1..333
                     /locus_tag="AC1PNG_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HIP6702597.1"
                     /translation="VFLTDSELHQLNERIAKSHCQNFSVYARKVLLNPNMSFVTINTD
                     TYEQLVFELRRIGNNINQIARAINQSHLISQEQLQELSKGVSELITGVEKEFQVEVKR
                     LKEFHGSH"
BASE COUNT          157 a           64 c           87 g          134 t
ORIGIN      
        1 gtatttttaa cagattcaga gttacatcaa ttgaatgaac gaattgctaa gagtcattgt
       61 caaaatttct cagtctatgc tagaaaagtt ctgcttaatc ccaatatgtc ctttgtcacg
      121 attaacactg atacctatga gcagttagtg tttgaattga gacggattgg aaataacatt
      181 aatcaaattg cgcgtgcgat caatcaaagc catctgattt cccaggaaca gttacaagaa
      241 ttaagtaagg gagttagtga attaattact ggagtagaga aagaatttca agtcgaagtg
      301 aaaagattaa aggagtttca tggtagtcac taaacacttt gcgacacatg gaaaaaaata
      361 tcgtaggcgt ttaattaagt atattctcaa tcctgataaa acggacaatt tgaaattggt
      421 atctgatttt ggcatgagca at
//
LOCUS       DBNFEG010000316          434 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.318, whole genome shotgun sequence.
ACCESSION   DBNFEG010000316 DBNFEG010000000
VERSION     DBNFEG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.318"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>434
                     /locus_tag="AC1PNG_002152"
     CDS             <1..>434
                     /locus_tag="AC1PNG_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIP6702598.1"
                     /translation="KAMEIFKFVSDKGYADLPILSASQELGMIRRDEIGIDIKYDKEA
                     VANYKRVLPGQFVIHLRSFQGGFAWSEIEGLTSPAYTILDFKEENSSKFWRNVLTSPN
                     FIKKLETVTYGIRDGRSISYSDFSTLNFVIPTLPEQEAIGSF"
BASE COUNT          141 a           70 c           95 g          128 t
ORIGIN      
        1 aaggcaatgg agatttttaa gtttgtgtct gacaaagggt atgcagattt accaattttg
       61 tcagcttctc aagaacttgg gatgattaga cgagatgaaa taggaattga catcaaatat
      121 gacaaggaag cggtagcgaa ttataagcgt gttttaccgg gtcaatttgt aattcatctt
      181 cgatcatttc aaggaggatt tgcatggtct gagatagagg gactgacatc acctgcctat
      241 acaatattgg attttaagga agaaaactcg tcaaaatttt ggcgaaatgt gttaactagt
      301 ccgaatttca taaaaaaact tgaaacagtt acatatggaa taagggatgg acggagcata
      361 agttattctg atttttcgac attaaatttt gtcatcccca cgctccccga acaagaagcc
      421 atcggcagct tctt
//
LOCUS       DBNFEG010000317          434 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.319, whole genome shotgun sequence.
ACCESSION   DBNFEG010000317 DBNFEG010000000
VERSION     DBNFEG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.319"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            104..418
                     /locus_tag="AC1PNG_002153"
     CDS             104..418
                     /locus_tag="AC1PNG_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SSU0592/SSU0593 family protein"
                     /protein_id="HIP6702599.1"
                     /translation="MKQLVTKRSYKIGLSAMALVLLMTTAGGATAHASTGTYSVETRG
                     DAEVYRLLETPAGSFTQGNVVLEPVNNSTTKPSETKSWFDSFWSSVKTFWEAICRGVG
                     WC"
BASE COUNT          125 a           64 c          102 g          143 t
ORIGIN      
        1 agttttttat ctaacctttt tctaaagttg gaatttgagt tgtctgtttg tgctatacta
       61 tcatttgtag agacgaatat cgtataaagg agatatttca acaatgaaac aacttgtaac
      121 aaaacgtagt tataaaattg gtttgagtgc tatggcttta gtcttgctaa tgaccactgc
      181 aggtggtgcc actgcacatg caagtacggg tacctatagt gtcgaaactc gaggtgatgc
      241 cgaagtgtat aggctgttgg aaactccggc ggggtctttt acacagggga acgtagtttt
      301 ggaacctgtg aataattcta caactaaacc atctgaaacc aaatcatggt ttgattcttt
      361 ttggagtagt gtaaaaacat tttgggaagc aatctgtcgt ggagttggat ggtgttaagt
      421 agaatgttta ctat
//
LOCUS       DBNFEG010000318          418 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.320, whole genome shotgun sequence.
ACCESSION   DBNFEG010000318 DBNFEG010000000
VERSION     DBNFEG010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.320"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            26..370
                     /locus_tag="AC1PNG_002154"
     CDS             26..370
                     /locus_tag="AC1PNG_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SSU0592/SSU0593 family protein"
                     /protein_id="HIP6702600.1"
                     /translation="MKHIVTKRNYKVGACAMALALLMTTAGGAIVNAEQRLNSPVGFE
                     QRDEVLLELKESTQPFGQGVQHLEPINSPATQMAPSVQKSSWFSSFWGGVKTFWNALC
                     CSVGLTGLKVSK"
BASE COUNT          131 a           71 c           85 g          131 t
ORIGIN      
        1 atctatataa aggagatgtt tcatcatgaa acatattgta acaaaacgta attataaagt
       61 tggcgcatgc gctatggcac ttgcattgtt gatgactact gcaggtggtg caattgtaaa
      121 tgcagagcaa cgacttaatt caccagttgg ttttgagcag cgtgacgaag tacttctaga
      181 gttgaaagaa tcaacccaac cttttggtca aggtgttcaa catttagagc ctatcaactc
      241 acctgcgact caaatggccc cttctgtaca aaaatcatca tggtttagtt cattttgggg
      301 tggtgtaaaa acattctgga atgcattatg ttgttctgtg ggactaactg ggctcaaagt
      361 tagtaaataa catagagtta taatcttgta atatttagtc aattaaattt gcaaatcc
//
LOCUS       DBNFEG010000319          413 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.321, whole genome shotgun sequence.
ACCESSION   DBNFEG010000319 DBNFEG010000000
VERSION     DBNFEG010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.321"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..>413)
                     /locus_tag="AC1PNG_002155"
     CDS             complement(<1..>413)
                     /locus_tag="AC1PNG_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent nuclease"
                     /protein_id="HIP6702601.1"
                     /translation="NIKKVAYTPDSELEPILGINLQIWIQYSEEDSYQNIQPLMMDLN
                     PDNNYIILDFSYIVPISRLHDLNTEISNFSDDFSKFESFMKKSMSKFFEMQINSRGYN
                     PDIQKLTEEKSDLLEMKDIHKLIKIRGIRANREVS"
BASE COUNT          133 a           66 c           56 g          158 t
ORIGIN      
        1 ttgatacttc tcgattagct ctaatccctc ttattttaat aagtttatga atatctttca
       61 tttcaagtaa atcagacttt tcttctgtca atttctgtat atcaggatta tatccccgtg
      121 agttaatctg catctcaaaa aatttactca tgctcttctt cataaatgac tcaaattttg
      181 aaaaatcatc tgaaaaattg cttatttcgg tatttaagtc atgaagtctc gaaataggga
      241 ctatatacga aaaatcaaga ataatataat tattatcagg atttaaatcc atcataagtg
      301 gctggatgtt ttgataagaa tcttcttctg agtactgaat ccaaatttga aggtttattc
      361 ctaaaatagg ttctaattcc gagtcagggg tatacgcaac cttttttata ttt
//
LOCUS       DBNFEG010000320          385 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.322, whole genome shotgun sequence.
ACCESSION   DBNFEG010000320 DBNFEG010000000
VERSION     DBNFEG010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.322"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>385
                     /locus_tag="AC1PNG_002156"
     CDS             <1..>385
                     /locus_tag="AC1PNG_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HIP6702602.1"
                     /translation="ERVEMYNVNFTNNDKLYESRNDRQEKHQQNIHAHHLIQSFSPED
                     NLTPEEINRIGYETMMELTGGRFRFIVATHTDKNHVHNHILINAIDRNSDKKLIWNYT
                     LERNLRMISDRISKVAGAKIIEKRFS"
BASE COUNT          140 a           77 c           66 g          102 t
ORIGIN      
        1 aagaaagggt agaaatgtac aatgtcaact ttaccaataa cgacaagttg tacgaatcta
       61 gaaatgaccg acaagaaaaa catcaacaaa atatccatgc ccatcacctc attcaatcat
      121 tttctcctga agataatctg acacctgaag aaattaaccg cattggttat gagaccatga
      181 tggaattaac agggggccgt tttcgtttca tcgtggcaac tcatacagac aaaaatcatg
      241 ttcataatca catcctaatc aacgccattg accgtaattc agataaaaag ttaatatgga
      301 actatacctt ggaacgaaac cttcgtatga tttcagatcg tatttctaaa gtggctggag
      361 cgaaaattat tgagaagcgt ttctc
//
LOCUS       DBNFEG010000321          385 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.323, whole genome shotgun sequence.
ACCESSION   DBNFEG010000321 DBNFEG010000000
VERSION     DBNFEG010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.323"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>385
                     /locus_tag="AC1PNG_002157"
     CDS             <1..>385
                     /locus_tag="AC1PNG_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HIP6702603.1"
                     /translation="EMVEMYNVNFTNNDKLYESRNDRQEKHQQNIHAHHLIQSFSPED
                     NLTPEEINRIGYETMMELTGGLFKFIVATHTDKDHTHNHILVNAIDSNSDKKLIWNYA
                     LERNLRMISDRISKMAGAKIIEKRYS"
BASE COUNT          147 a           73 c           65 g          100 t
ORIGIN      
        1 aagaaatggt agaaatgtac aatgtcaact ttaccaataa cgacaagttg tacgaatcaa
       61 gaaatgaccg acaagaaaaa catcaacaaa atattcatgc ccatcacctc attcaatcat
      121 tttctcctga agataatctg acacctgaag aaattaaccg cattggttac gagactatga
      181 tggaattgac aggaggtctc tttaagttta tcgtagcgac tcatacagac aaagatcata
      241 cccacaacca tatcttagtc aatgccatag acagtaattc agataaaaaa ttgatatgga
      301 attatgcctt agaacgaaat ttacgtatga tttcagaccg tatttctaaa atggcggggg
      361 caaaaattat cgagaagcgt tactc
//
LOCUS       DBNFEG010000322          373 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.324, whole genome shotgun sequence.
ACCESSION   DBNFEG010000322 DBNFEG010000000
VERSION     DBNFEG010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.324"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>373
                     /locus_tag="AC1PNG_002158"
     CDS             <1..>373
                     /locus_tag="AC1PNG_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIP6702604.1"
                     /translation="VVFGIDVSKVSSEVAILVNGEKVHNYTMSNDAIGFSRLLGDLKT
                     VHKPEIIFEATGVYSRRLQSFLDEHGYAYTRLNPLEAKKQLDSLRVRKTDQIDAEKLA
                     QSQFVLNRKTTYVQEEIYQNLR"
BASE COUNT          117 a           81 c           79 g           96 t
ORIGIN      
        1 gtagtatttg ggattgacgt gagtaaagta agttcagaag tagccattct agtcaacggc
       61 gagaaggttc ataactacac catgtccaat gatgccattg gcttttctcg gctacttggc
      121 gatttgaaaa ccgtccacaa gccagaaatc atctttgaag caacaggcgt ctattctcgt
      181 cgtcttcaat cttttctgga tgaacatggc tacgcttata cacgactcaa tcccttagaa
      241 gccaagaagc aactggatag cttgcgtgtg agaaaaacag atcaaattga cgccgaaaaa
      301 ctagctcaat ctcagtttgt actgaatcgt aaaacgacgt atgtccaaga agaaatctac
      361 caaaacttgc ggg
//
LOCUS       DBNFEG010000323          361 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.325, whole genome shotgun sequence.
ACCESSION   DBNFEG010000323 DBNFEG010000000
VERSION     DBNFEG010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.325"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            30..>361
                     /locus_tag="AC1PNG_002159"
     CDS             30..>361
                     /locus_tag="AC1PNG_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HIP6702605.1"
                     /translation="MEQLNLITNFLRIKDKNIVITDEYDMGTHLELHGHLDYTAPKCP
                     SCKGQMTKYDFQKASKIPYLETAGYPLLIRLRKRRFKCKDCGKIAVAETPLVKKNHQI
                     SVAVNQKIA"
BASE COUNT          130 a           86 c           57 g           88 t
ORIGIN      
        1 cgactaaaca actcattaga aagactcata tggaacaact aaatcttatc acaaattttc
       61 tcagaattaa agataaaaat atcgttatca ctgatgaata tgatatggga actcacttag
      121 aactccacgg tcacttggat tacacagccc ctaaatgccc ttcctgcaag ggacaaatga
      181 caaaatacga tttccaaaaa gcctccaaaa ttccctacct agaaacagct ggctatccct
      241 tacttatccg acttagaaag cgtcgcttca agtgtaaaga ttgcggaaaa atagcggttg
      301 ctgaaactcc tcttgtcaag aagaaccacc aaatctctgt cgcggttaac cagaaaatcg
      361 c
//
LOCUS       DBNFEG010000324          359 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.327, whole genome shotgun sequence.
ACCESSION   DBNFEG010000324 DBNFEG010000000
VERSION     DBNFEG010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.327"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            106..>359
                     /locus_tag="AC1PNG_002160"
     CDS             106..>359
                     /locus_tag="AC1PNG_002160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIP6702606.1"
                     /translation="MVTISACSGSNEIIGTWQAQVADGTELIIEIDKKQIAVYGETSD
                     YVHNGFGFENNMSYYTIEVNEWNIYTIIFPAKDKSIAVLI"
BASE COUNT          123 a           42 c           77 g          117 t
ORIGIN      
        1 tgggctatat agtgtattaa atagtctatg gggacagtta gaaaacttat ttaattaagg
       61 aaaagttatg aaaaattagt cactattatt ttatttttca tagggatggt gactatatct
      121 gcttgtagtg gttctaatga aatcatcgga acatggcaag cacaggtagc agatgggaca
      181 gagctgataa ttgaaattga caaaaaacag attgcggtat atggtgagac ctcggactat
      241 gttcacaatg gatttggttt tgaaaataac atgagttatt atactataga ggttaatgag
      301 tggaacatct atacgattat ttttcctgca aaagataaat caattgccgt cttgattca
//
LOCUS       DBNFEG010000325          358 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.328, whole genome shotgun sequence.
ACCESSION   DBNFEG010000325 DBNFEG010000000
VERSION     DBNFEG010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.328"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..239
                     /locus_tag="AC1PNG_002161"
     CDS             <1..239
                     /locus_tag="AC1PNG_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HIP6702607.1"
                     /translation="NRLKKHMFWIGLISLVLMLVDNVLVCYFNIECSRTLNQIEQTIC
                     IILSILVFLGVLNNKPDVKVEDIMKNGMSSKNQK"
BASE COUNT          120 a           44 c           63 g          131 t
ORIGIN      
        1 agaatcgttt gaaaaaacat atgttttgga ttggactaat ttcgttggta ttgatgctcg
       61 ttgataatgt cctggtttgt tatttcaata ttgaatgtag tcgtacttta aaccaaatcg
      121 agcagactat ttgtattatc ctcagtattc ttgtattttt aggagtgcta aataataaac
      181 cggatgtgaa ggtagaagac atcatgaaaa acggtatgtc atctaaaaat caaaaataat
      241 gtttgtttag aaactactga tgaggtatct agttgtactt acagttcagt atctctagct
      301 aagatggatt tttcaaaata tagtcagtat ctcctaaaaa actattttgt atataaat
//
LOCUS       DBNFEG010000326          349 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.329, whole genome shotgun sequence.
ACCESSION   DBNFEG010000326 DBNFEG010000000
VERSION     DBNFEG010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.329"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
BASE COUNT          106 a           74 c           69 g          100 t
ORIGIN      
        1 agaaaaagag ttcgtcaaca agtctttatt tctagttgtt gagctgaaac agtctatccc
       61 cagactgttt cactcccacc cccgcatagt tgattaaatc agattcggag tatgaaatac
      121 gaattgctga aatttgtttt ggaactccta tctccaacct ttaacagtcc actgaactgt
      181 taaagccaat caatcactgc gctaagatgt tgatatgaac tttgaggagt ggatctgggc
      241 tttgcccagc ctctcttctt ttggagaagc gatagagaaa ggacttgtta tgcaaacaat
      301 attcggcaaa atagtagcca atcagtcagc agagtcggtc aaaacctat
//
LOCUS       DBNFEG010000327          342 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.330, whole genome shotgun sequence.
ACCESSION   DBNFEG010000327 DBNFEG010000000
VERSION     DBNFEG010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.330"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
BASE COUNT          127 a           77 c           37 g          101 t
ORIGIN      
        1 aaattcgagt atcttcattc cccaaacaat aaaatatact taacacaaat atgattttct
       61 tcttcgattt tatcaatcat tagtaaacat tttacatgtc attattcata tattatccaa
      121 aattggctat actacattca cgaaagtaaa ccatacaaaa tgggagcggg aagtgatacg
      181 actgactccc tcactaccaa tttgccacct caaacaatct ccagaatatt cgaaaactca
      241 accactccgc cgagatgtta ataggaactc aaacattaac atttttaccc tgtgtttact
      301 actctaaata aaaaataccc tattgcaacg tgcaacaagg ta
//
LOCUS       DBNFEG010000328          338 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.331, whole genome shotgun sequence.
ACCESSION   DBNFEG010000328 DBNFEG010000000
VERSION     DBNFEG010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.331"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>338
                     /locus_tag="AC1PNG_002162"
     CDS             <1..>338
                     /locus_tag="AC1PNG_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702608.1"
                     /translation="HINLEELKNNSNLLLKEHSKVKKEKQEYDKLISEQISQIVTLKK
                     PLLELILPHKNFHPFELGHREIRYYAAPIPIENTVYIDFISNTYGKSKSSFFSDFAYV
                     VSVRVTQTNK"
BASE COUNT          137 a           51 c           48 g          102 t
ORIGIN      
        1 tcatattaat ttggaggaat taaaaaataa tagtaatttg ttgctaaaag aacactccaa
       61 agtaaaaaaa gaaaaacaag aatatgataa attgataagc gagcagattt ctcaaattgt
      121 tacgttaaaa aaaccattgc ttgaacttat tttaccccac aaaaactttc atccattcga
      181 actaggtcat cgagaaataa gatactatgc ggcgccaatt cctatcgaaa acacggtata
      241 tatagatttt atttctaata catatggtaa aagtaaaagc tcattctttt ctgattttgc
      301 ttatgtcgta tcggtaagag taactcagac aaataaaa
//
LOCUS       DBNFEG010000329          335 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.332, whole genome shotgun sequence.
ACCESSION   DBNFEG010000329 DBNFEG010000000
VERSION     DBNFEG010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.332"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..218
                     /locus_tag="AC1PNG_002163"
     CDS             <1..218
                     /locus_tag="AC1PNG_002163"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702609.1"
                     /translation="ITYQEKTPSEYTKMLIDLYDLTTPRMANIEISKVRADEKTFAYI
                     LTYSLYDETNISIKRVFSFVMEDKTDE"
BASE COUNT          118 a           40 c           74 g          103 t
ORIGIN      
        1 agattaccta ccaagagaag actccttcgg aatacacaaa gatgctgata gatttatatg
       61 atttaactac cccaagaatg gcaaatattg aaattagtaa agtaagggct gacgaaaaaa
      121 catttgcata tatattaaca tattcattat atgatgagac taatatttca attaaacgtg
      181 tcttttcttt tgtgatggag gataaaacag atgagtaggg ctgatatcaa tcgtcgggtg
      241 gaggtatctg ccgatattgc agacagtgtt tataagtttg aagagtattt tgtaaagaat
      301 agcgatatat tatgggatgg gtagtgtagt aaggc
//
LOCUS       DBNFEG010000330          334 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.333, whole genome shotgun sequence.
ACCESSION   DBNFEG010000330 DBNFEG010000000
VERSION     DBNFEG010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.333"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            106..>334
                     /locus_tag="AC1PNG_002164"
     CDS             106..>334
                     /locus_tag="AC1PNG_002164"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIP6702610.1"
                     /translation="MEQVIISFKKKSDGELLRYSYQNRTGICIPFSDILFIQTSVKPH
                     RLIIYTVDFIKEFYGTLNDILDLDSKNYFEKV"
BASE COUNT          117 a           43 c           51 g          123 t
ORIGIN      
        1 tattccgacc tgatgcccct agtatttcaa tatcatcttt cggcattaga ttttattatt
       61 aaagatctta aagaaacaga atttcgtaat cggttaaagc agtgtttgga acaagtaata
      121 atttcattta agaaaaaatc tgatggggaa ttgttaagat atagttacca gaacagaact
      181 ggtatctgca tacctttttc cgatatactt tttattcaaa cctcagttaa accgcatcga
      241 ttaattattt atacagtaga ttttattaaa gaattttatg gtactttgaa tgatattcta
      301 gatttagata gtaagaatta ttttgaaaaa gtag
//
LOCUS       DBNFEG010000331          332 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.334, whole genome shotgun sequence.
ACCESSION   DBNFEG010000331 DBNFEG010000000
VERSION     DBNFEG010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.334"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..303
                     /locus_tag="AC1PNG_002165"
     CDS             <1..303
                     /locus_tag="AC1PNG_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6702611.1"
                     /translation="FTFLDLERHFDVYNDNSMDLKTFGKGEKFFRVKCKLPSINQAKL
                     KMAVSVRNSNKQPLLFAKTSDTPAIFISRKFSTDNIAEEDAATGFIQRNSQWELLD"
BASE COUNT          117 a           47 c           70 g           98 t
ORIGIN      
        1 tttacatttt tagatttaga acgtcatttt gatgtatata acgacaattc tatggattta
       61 aaaacatttg gtaaaggcga gaagtttttc cgagtgaaat gtaagctacc ttcaattaat
      121 caggcaaaat taaaaatggc ggtgtcagta cgaaatagta ataagcagcc gttattattt
      181 gccaagacat ctgacacacc agctattttt attagtagga agtttagtac tgataatatt
      241 gcagaggaag acgcagcaac cgggtttatt cagagaaata gccaatggga gctgctagat
      301 taataaaata taacggaggc agaatgtcgc gt
//
LOCUS       DBNFEG010000332          319 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.336, whole genome shotgun sequence.
ACCESSION   DBNFEG010000332 DBNFEG010000000
VERSION     DBNFEG010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.336"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..268
                     /locus_tag="AC1PNG_002166"
     CDS             <1..268
                     /locus_tag="AC1PNG_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HIP6702612.1"
                     /translation="DAITLDQLKSRSVSLKSKKSNKGIKVDFKDFPHLIIWSTTNQSP
                     FIALEPWSGLSTSLEESDILEEKQGVTFVEPSQTRIKSFDITIL"
BASE COUNT          121 a           42 c           59 g           97 t
ORIGIN      
        1 cgatgctatt accctagatc aactaaagtc acgtagtgtc agtttgaagt ctaaaaaaag
       61 taataaggga attaaggtgg attttaaaga ttttccacat ttaattattt ggtccaccac
      121 taatcaaagt ccatttatag ccttagagcc atggagtgga ttatcaacat cattagagga
      181 atcagatata ttggaagaga agcagggggt tacatttgta gaaccaagtc aaactagaat
      241 caaatcattt gatattacta ttttgtaaat taaagtaatc tgaatggaat ataaagatag
      301 ggactttaga aatttacaa
//
LOCUS       DBNFEG010000333          314 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.337, whole genome shotgun sequence.
ACCESSION   DBNFEG010000333 DBNFEG010000000
VERSION     DBNFEG010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.337"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
BASE COUNT          100 a           57 c           47 g          110 t
ORIGIN      
        1 tttaactgtt ctatcatata ttctatacta gttatctaac agataatttg aacacaagct
       61 atcctcatat caccaaagac tgagtggacc cctactagtc atgaccaccc gtgaaaacgg
      121 gtggtttatt ttgtgtaatt tagagaaaag agtgccaaaa aacgcataaa atcgtgttgc
      181 taagaacgtt gattttatgc gttttattat atgacttatc tcgatatttt tattgaaatt
      241 tatgtttatg cccaactata cttctcagaa tcaatgtcca tatctcatct cagcctagta
      301 tagattgaca gatt
//
LOCUS       DBNFEG010000334          306 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.338, whole genome shotgun sequence.
ACCESSION   DBNFEG010000334 DBNFEG010000000
VERSION     DBNFEG010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.338"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..>306)
                     /locus_tag="AC1PNG_002167"
     CDS             complement(<1..>306)
                     /locus_tag="AC1PNG_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HIP6702613.1"
                     /translation="NEQLTVQFSEFGGALSSIKDKDGVEYLWQGNPEYWSGQAPVLFP
                     ICGSLRNDWAIYRQAERPHFTGIIPRHGLVRKENFDYCKIDDSTVSFGFKANEKTLKE
                     "
BASE COUNT           94 a           70 c           38 g          104 t
ORIGIN      
        1 ttctttaagt gttttttcat tagccttaaa accaaaggac actgtgctgt catctatctt
       61 acaataatca aaattttcct ttctaactaa tccatgtcgt ggaataattc cagtaaaatg
      121 aggtctttct gcttgtctgt atattgccca atcattcctc aaacttccac aaatcggaaa
      181 taaaactggc gcttgtccac tccaatattc tggattacct tgccaaaggt attcaactcc
      241 atctttatct ttaatagaag ataaagcacc accaaactcc gaaaactgta ctgttaactg
      301 ttcatt
//
LOCUS       DBNFEG010000335          292 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.340, whole genome shotgun sequence.
ACCESSION   DBNFEG010000335 DBNFEG010000000
VERSION     DBNFEG010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.340"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>292
                     /locus_tag="AC1PNG_002168"
     CDS             <1..>292
                     /locus_tag="AC1PNG_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6702614.1"
                     /translation="SKQRTIFINKRAKQKTVKLTPKTVVKNHPQKSSVEKRRQRYLDK
                     VFSKNPNIQVLEYIDSKTPVHYKCCQCQFEWKARSDKIFIRPYCKNCKSISI"
BASE COUNT          125 a           45 c           43 g           79 t
ORIGIN      
        1 aatcaaagca acgaactatt tttattaaca aaagagctaa acaaaagaca gtaaaattaa
       61 cgccgaaaac agtagtaaaa aatcatcccc aaaaatcttc ggtagaaaaa aggaggcaga
      121 gatatttgga taaagttttc tcaaaaaatc caaatattca ggttttagaa tatattgatt
      181 caaaaactcc tgtccattat aaatgttgtc aatgccaatt tgaatggaag gcaagaagtg
      241 ataaaatatt cattcgtccc tattgtaaga attgcaaatc aatctccatt ga
//
LOCUS       DBNFEG010000336          287 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.denovo.341, whole genome shotgun sequence.
ACCESSION   DBNFEG010000336 DBNFEG010000000
VERSION     DBNFEG010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.denovo.341"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..>287)
                     /locus_tag="AC1PNG_002169"
     CDS             complement(<1..>287)
                     /locus_tag="AC1PNG_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIP6702615.1"
                     /translation="LFNLINLDFEIVKMKAFKNYVYVGNFIFAFMLLSITIWTLILQS
                     NLSVIKIFGPTVALAHFLLFLFLAQYMKSQQRIYEVNLEVIRREEENRNLN"
BASE COUNT          116 a           40 c           34 g           97 t
ORIGIN      
        1 attaagattt ctattctctt cttcacggcg aattacttct aaatttactt cgtaaattcg
       61 ttgctgtgat ttcatatatt gggctaaaaa aagaaataga agaaaatggg ctaatgcaac
      121 tgtaggtcca aatatcttga taactgataa attagattgt aatataagcg tccatattgt
      181 aatagacaaa agcataaaag caaatataaa attcccaaca taaacataat ttttaaatgc
      241 tttcatctta actatttcaa aatctaaatt gattaaatta aataaca
//
LOCUS       DBNFEG010000337         3111 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.guided.188, whole genome shotgun sequence.
ACCESSION   DBNFEG010000337 DBNFEG010000000
VERSION     DBNFEG010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.guided.188"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1371
                     /locus_tag="AC1PNG_002170"
     CDS             <1..1371
                     /locus_tag="AC1PNG_002170"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6702616.1"
                     /translation="TDVDGLMEMVGYGLMIVVYAGGMLAFIIPTMFFIDWKISLVALL
                     PMLFMTLCIFFIGRKQDKAIDANREAVAQLNNEVLEVIEGIRVTRAYSKKATQKAQFQ
                     ARTKQLAQGGDRITSLQSLYNPLATVCLGLSTIFVLLMGAQAVKAGQLTLGQVIALQL
                     YVGSLLEPFWTLADFILVYQTGKTSFEKLQELIETGDDLEADGSKEIAELSSISFKNY
                     SFSYPQAERASLQDINWTLKAGQTVGIVGKTGSGKTTLVRQFLRQYPIGQGNFFINHQ
                     SVLDFKRSSIEEKIGYVPQEHILFSRSVGENIALGKVASSSEEIEQAIATAAFSQDLK
                     RMTDGLDTMIGERGVSISGGQKQRISIARAFLREPDLLILDDSLSAVDARTERQIIQN
                     IQKERAGKTNVIVTHRLSAVNHADWVLVLDEGRIVEEGRPADLLAQRGWYYEQYQRQQ
                     SQEGGE"
     gene            1371..3110
                     /locus_tag="AC1PNG_002171"
     CDS             1371..3110
                     /locus_tag="AC1PNG_002171"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6702617.1"
                     /translation="MTILGFLLKQIRRVKWLFIVAVGFYLLASTMVRLAPLLIQQAID
                     GPITDLSKGLPFDEAVFLNQSAQYMGMIILGAIGFYLSMRLLMHCANRIAENLRNQAY
                     DVMQRLPISYFDDKPAGKIATRIVNDTETLRTQFYGTLVNAFNNIVRLLFTYGVLFYM
                     NRSLGWLMLLLIPLYIGIQFAYKKMTDKPMKDFYDARSDVNTQVNETMNGASLIQLFG
                     QEERIMKEFEVTADKMRRADNKIIWAQSLATWNLSGFLQNLVIAAILTVVGYQFLAGQ
                     YGVTAGKLFVYVNYIEGVFIALGALVQQFPNMLRLFETGKRLMALLEEEVEDDCDRIL
                     EVEQGQVVFEHVNFSYEENRPVLKDITIRAEKGETVALVGHTGSGKSSIMNLLYRFYD
                     PQEGRVLIDGKNIRDYSRESLRSHMGIVLQDPYLFTGTIASNVSMNEEEADRTRIMQA
                     LEKVGAGPMLSRLEKGIDEPVVEKGAAFSSGERQLIAFARTLYSDPKILILDEATSHI
                     DTETEEIIQHAMEVVKEGRTTFIIAHRLSTIQNADQILVLDQGRIIEHGKHEELVARG
                     GVYAQMHEIQAKV"
BASE COUNT          859 a          567 c          766 g          919 t
ORIGIN      
        1 acggatgtgg atggcctgat ggagatggtg ggttacggtc ttatgattgt cgtctatgca
       61 ggcggtatgt tggcttttat cattcccacg atgttcttta ttgattggaa aatttcgcta
      121 gtagctttac taccgatgct gttcatgaca ctatgtatat tttttatcgg gagaaaacaa
      181 gataaggcga ttgatgccaa tagagaagct gtagcccagc tcaataatga agttttggag
      241 gtcatcgaag gcattcgagt gactcgggct tatagtaaaa aagcaactca aaaggctcaa
      301 tttcaagctc gtaccaagca attggctcaa ggtggggatc gcatcacttc cttgcaatca
      361 ttgtataatc cattagccac agtctgtttg ggattgtcta ctattttcgt gcttctcatg
      421 ggggcacaag ccgtgaaagc agggcaactg accttgggac aagtaattgc ccttcaattg
      481 tatgtcggtt cgcttttgga gccgttttgg acactagcag atttcatcct tgtttatcag
      541 actggtaaga catctttcga aaaactgcaa gagctgattg agacgggtga tgatttggag
      601 gctgatggtt ctaaggaaat tgcagagctg tctagtattt ccttcaaaaa ttatagcttt
      661 agttatccac aggcggaaag agccagccta caggacatca attggacact gaaagcaggg
      721 caaactgtag gtatcgttgg gaaaactggt tcgggaaaaa cgaccttggt tcgccaattc
      781 ttacgccagt atccgattgg tcagggaaat tttttcatca atcatcaatc tgttttagac
      841 tttaaacgtt ctagtataga ggaaaaaatt ggctatgttc cccaagaaca cattttgttt
      901 tcaaggtctg ttggagaaaa tattgccctt gggaaagtag ctagtagctc tgaggagatt
      961 gaacaagcca ttgccacagc agcttttagt caagatttaa aacggatgac tgatggtctt
     1021 gataccatga ttggtgagcg tggtgtgtct atttctggcg gtcagaaaca acggatttcc
     1081 attgcccgtg ctttcctgcg agagccagat ttgctgattc tagatgattc tttgtcggca
     1141 gtggatgcta ggacagaacg ccagattatc caaaatattc aaaaagaacg tgcaggcaaa
     1201 accaatgtca tcgtgaccca tcgtctatct gctgtcaacc atgcagactg ggtactagtc
     1261 ttggatgagg ggcgtatcgt tgaggaaggg cgtccagctg atttgcttgc ccaaagaggt
     1321 tggtactatg aacaatacca acgacaacaa agtcaggaag gaggagagta atgactattt
     1381 taggtttttt attaaaacaa atccgtcgtg ttaagtggct atttatagta gctgtcggct
     1441 tctatctatt ggcttcgacc atggttcgat tggctcctct actgattcag caggcgattg
     1501 atgggccgat tacggatttg agcaagggtt tgccttttga tgaagctgtt ttcttaaacc
     1561 agtcagctca atacatggga atgattattc tgggagctat tggcttctac ttatctatgc
     1621 gtctactcat gcattgcgcc aatcgtattg ctgaaaatct acgtaatcaa gcttatgatg
     1681 ttatgcaacg gttgcctatt tcttattttg atgataagcc cgctggaaaa attgcaacta
     1741 gaattgtcaa tgatacagaa actctgcgga cacaatttta tgggacctta gttaatgcct
     1801 ttaacaatat tgttcgtttg ctctttacat acggtgtcct attttacatg aataggagtc
     1861 ttggttggct gatgttactc ttgataccac tctatatcgg tattcaattt gcttacaaga
     1921 aaatgacaga caagccgatg aaggattttt atgatgcaag gagtgacgtc aatacgcagg
     1981 tgaatgagac catgaatggt gcaagtttga ttcaactttt tggacaagaa gagcgcatca
     2041 tgaaagaatt tgaagtgact gctgacaaga tgcgtcgggc agataataag attatctggg
     2101 cccagtctct tgccacgtgg aatctgagtg gttttcttca aaatttggtc attgctgcga
     2161 ttttaacggt agtcggctat cagtttcttg cgggacagta tggagttaca gctgggaaac
     2221 tctttgttta tgtcaactat atcgaaggag tcttcattgc tttgggagcg ctggttcaac
     2281 agttcccaaa tatgcttcgc ttgtttgaaa caggtaagcg tctaatggcc cttctagaag
     2341 aagaggtgga ggacgactgt gatcgtattt tggaagttga acaagggcaa gtcgtgtttg
     2401 aacacgtgaa ctttagctat gaagaaaaca gaccagtttt gaaagatatt accattcggg
     2461 cagaaaaagg agaaacagtt gctctggttg ggcatactgg gtctggtaag tcttctatta
     2521 tgaacctcct ctatcgtttc tatgatccac aggagggacg tgtactgatt gatgggaaaa
     2581 acatccgtga ttattctcgt gagagcctgc gcagtcacat ggggattgtt ttgcaagatc
     2641 cctatctctt tacagggacg attgctagca atgtttccat gaatgaagag gaagcagata
     2701 gaacaagaat catgcaggcg ttagaaaaag ttggtgcagg gcctatgcta tctcgcttag
     2761 agaaagggat tgatgaaccg gttgttgaga agggggcggc cttttcaagt ggggaacgtc
     2821 agttgattgc ctttgcgcgg acactctact ctgatcctaa aattctgata ttggatgaag
     2881 caacttctca tattgatacg gagacggaag aaattatcca acatgctatg gaagtggtga
     2941 aagaaggtcg gacaaccttt atcattgccc accgactttc taccattcaa aatgcagacc
     3001 agattcttgt cttagatcaa ggccgaatta ttgaacatgg gaagcatgaa gaattggttg
     3061 cacgtggtgg agtatatgct caaatgcatg agattcaagc aaaggtgtaa t
//
LOCUS       DBNFEG010000338         2363 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_52
            SAMN40446058-rid29426023.guided.210, whole genome shotgun sequence.
ACCESSION   DBNFEG010000338 DBNFEG010000000
VERSION     DBNFEG010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446058
            Sequence Read Archive: SRR28339469
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171409.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:59:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,072
            CDSs (with protein)               :: 2,072
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 30 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 10 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446058-rid29426023.guided.210"
                     /strain="DA1_52"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            399..2318
                     /gene="tet(O)"
                     /locus_tag="AC1PNG_002172"
     CDS             399..2318
                     /gene="tet(O)"
                     /locus_tag="AC1PNG_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(O)"
                     /protein_id="HIP6702618.1"
                     /translation="MKIINLGILAHVDAGKTTLTESLLYTSGAIAEPGSVDKGTTRTD
                     TMNLERQRGITIQTAVTSFQWEDVKVNIIDTPGHMDFLAEVYRSLSVLDGAVLLVSAK
                     DGIQAQTRILFHALQTMKIPTIFFINKIDQEGIDLPMVYQEMKAKLSSEIIVKQKVGQ
                     HPHINVTDNDDMEQWDAVIMGNDELLEKYMSGKPFKMSELEQEENRRFQNGTLFPVYH
                     GSAKNNLGIRQLIEVIASKFYSSTPEGQSELCGQVFKIEYSEKRRRFVYVRIYSGTLH
                     LRDVIKISEKEKIKITEMCVPTNGELYSSDTACSGDIVILPNDVLQLNSILGNEMLLP
                     QRKFIENPLPMLQTTIAVKKSEQREILLGALTEISDGDPLLKYYVDTTTHEIILSFLG
                     NVQMEVICAILEEKYHVEAEIKEPTVIYMERPLRKAEYTIHIEVPPNPFWASVGLSIE
                     PLPIGSGVQYESRVSLGYLNQSFQNAVMEGVLYGCEQGLYGWKVTDCKICFEYGLYYS
                     PVSTPADFRLLSPIVLEQALKKAGTELLEPYLHFEIYAPQEYLSRAYHDAPRYCADIV
                     STQVKNDEVILKGEIPARCIQEYRNDLTYFTNGQGVCLTELKGYQPAIGKFICQPRRP
                     NSRIDKVRHMFHKLA"
BASE COUNT          765 a          387 c          543 g          668 t
ORIGIN      
        1 agattgtgga aaaggaaatt atttttgcat aaacagatga ttagtggcag ggggaaatcc
       61 tgccgctttt tctgctttag tttgtcagct tgacaaataa agggttaagg aatataatta
      121 gattcagtat tatacaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc
      181 aatttgatag tgggaacaaa aagtagcagt cccgtttcac ttttaatatg gggcttagtt
      241 ttttgtaccc agtttaagaa tacttttatc atgtaatttt atatgcccga aaacatataa
      301 gtgttttggg gctattggag ttatttaccc agtgatagga gtatttatca ctgggtattt
      361 ttatgccctt ttttgggtgt tgataggagg aaaatcacat gaaaataatt aacttaggca
      421 ttctggctca cgttgacgca ggaaagacaa cattaacgga gagtttattg tataccagtg
      481 gtgcaattgc agaaccaggg agcgtagata aaggcacaac aaggacagat acaatgaatt
      541 tggagcgtca aaggggaatc actatccaga cagcagtgac atcttttcag tgggaggatg
      601 taaaagtcaa cattatagat acgccaggcc atatggattt tttggcggaa gtataccgtt
      661 ctttatccgt attagacgga gcagtattat tagtttctgc aaaggatggc atacaggcac
      721 agacccgtat actgtttcat gcactacaga caatgaagat tccgacaatt tttttcatca
      781 ataaaattga ccaagagggg attgatttgc caatggtata tcaagaaatg aaagcaaagc
      841 tttcttcgga aattatagtg aagcaaaagg ttgggcagca tccccatata aatgtaacgg
      901 acaatgacga tatggaacag tgggatgcgg taattatggg aaacgatgaa ctattagaga
      961 aatatatgtc agggaaaccg tttaaaatgt cagaactgga acaggaagaa aacaggagat
     1021 tccaaaacgg aacgttattt cccgtttatc acggaagtgc taaaaacaat ctggggattc
     1081 ggcagcttat agaagtgatt gccagtaagt tttattcatc aacgcctgaa ggtcaatctg
     1141 aactatgcgg gcaggttttt aagattgaat attcagagaa aaggcggcgt tttgtttatg
     1201 tgcgtatata tagcggaaca ttgcatttga gggatgttat taaaatatct gaaaaagaga
     1261 aaataaaaat cacagagatg tgtgttccga caaacggtga attatattca tccgatacag
     1321 cctgctctgg tgatattgta attttaccaa atgatgtttt gcagctaaac agtattttgg
     1381 ggaacgaaat gctgttgccg cagagaaaat ttattgaaaa tcctctccct atgctccaaa
     1441 caacgattgc agtaaagaaa tctgaacagc gggaaatatt gcttggggca cttacagaaa
     1501 tttcagatgg cgaccctctt ttaaaatatt atgtggatac tacaacgcat gagattatac
     1561 tttctttttt ggggaatgtg cagatggaag tcatttgtgc catccttgag gaaaaatacc
     1621 atgtggaggc agaaataaaa gagcctactg ttatatatat ggaaagaccg cttagaaaag
     1681 cagaatatac catccacata gaagtcccgc caaatccttt ctgggcttct gtcgggttgt
     1741 ccatagagcc gctccctatt ggaagcggag tgcagtatga aagcagagtt tcacttggat
     1801 atttaaacca atcgttccaa aatgcggtta tggagggggt tctttatggc tgcgagcagg
     1861 ggctgtatgg atggaaagtg acagactgta aaatctgttt tgaatatgga ttgtattata
     1921 gtcctgtaag tacccccgca gactttcggc tgctttcccc tatcgtattg gagcaggctt
     1981 taaaaaaagc agggacagaa ctattagagc catatctcca ctttgaaatt tatgcaccgc
     2041 aggaatatct ctcacgggcg tatcatgatg ccccaaggta ttgtgcagat attgtaagta
     2101 ctcaggtaaa gaatgacgag gtcattctga aaggagaaat ccctgccaga tgtattcaag
     2161 aatacaggaa cgatttaact tatttcacaa atgggcaggg agtctgcttg acagagttaa
     2221 aaggatacca gccagctatt ggtaaattta tttgccaacc ccgccgcccg aatagccgta
     2281 tagataaggt tcggcatatg ttccacaagt tagcttaaca gcttgcaaaa gtcatataaa
     2341 atgagatttg aaaggattag aga
//
